sperm surface protein sp17, snap_masked-scaffold101_size371023-processed-gene-0.7 (gene) Tigriopus kingsejongensis
|
Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of sperm surface protein sp17 vs. L. salmonis genes
Match: EMLSAG00000001773 (supercontig:LSalAtl2s:LSalAtl2s1300:53330:57381:1 gene:EMLSAG00000001773 transcript:EMLSAT00000001773 description:"maker-LSalAtl2s1300-snap-gene-0.32") HSP 1 Score: 290.041 bits (741), Expect = 1.642e-80 Identity = 332/1248 (26.60%), Postives = 531/1248 (42.55%), Query Frame = 0
Query: 576 AATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMK----------PVEAEPVPEDDTFDIDLEDP------EVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDM-------------EDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDI-------DLTDPEVDRA---------ATKIQAGYKGMRERKKLR-ENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFP-EESPET---------GLTE----------DQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSM----------REKAEEQPDAMDIDLTDPEVE---------RAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEME-----------------------QAATKIQAGYRGMRTRRGMKPIPDVEVVV---------GEEITPRSESEG-DLSKMDPQNELVEGELDHAASAAEV--DAYGEPI-------NIDMTAPDMEQ------AATKIQAGFRGMQARK----GIQGRVAKAEDAFDIDLND-PEVEAAASKIQAGYMGMRTRRDLAARRPDI-----------------------ETVESNEEVIIGSYPEDDET------------------------------------ISAATKIQAGFKGMKARRDLSQLRQNRDLMDRDE--DSQTVISDIQETLIVATHVDALGAIPFDMHPSAVDGPE-DDEDEDKDKKGKNRQSSAGSEREDETFIAVGEESPSKVMRQDSVMRSSSLDASSSSLKPKNDLPPIMLYHYSRSYNSQKVVIYLRERGIQYSGYHVDLQRNEHLSNWFLDLNPKGEVPVMKFDNQVIVDSS-RIIHHLEAHVPGETY-PLLVPLSSETLLYQQYLYFAALLDNIPLNQVVQGVAFHPELSLAQGRSEPYDEPQVFEKIQQTAIRRGLVLRQTAES-NEKAASDLRKRASQFEKEILPNVKDRSAFEDTLDQVESILEKVEAQLSTTMHLEDECQGWLLGSSFSALDITLGVSLNRLAQLGLQPRFWLGDPARPHLLQFLEEI-QKRPHFQ 1619
AATK+Q Y ++R+ + K I+ DL + QA + T + ++ +E E G + DLN+ ATR+QA YRG +TR+ +K PV D +I E+ E+ ATK+QA Y G + R+ L + +E+ ++ ED + AATKIQA +RG +TR+++ ++E ++ PI+I D+T EV R T+++A Y+G + +K+ +NQ + L +AATK Q+ R ++ + + ++ I ++E N+ I + D D+E+ AT++QA Y+G KTRK L A +E+ E+ E ++E ++AA KIQ+ YRG + RKE+ + D + AATKIQA Y G +TR+ + + + + D D E+E ++ATK+QA Y+G + R+EL E+++ E++ ++ATK+Q+ YRG + R+ + E+V G +T R S D++ + Q + E L +A + + +PI N++ + ++ Q AAT IQA FRG RK G +K D I+ + ++ AAS IQA + G ++ D ++ ++ E E VI S EDD T AA KIQ+ F+G K R+ + R+ L D + + VI+DI GA+PF S+ P+ DD D D N +S G G+ P + I ++HY S S+KV++YL ERGI++SGYH+DL NE+ +WFL LN KGE+P M F+ IV ++ II LE+++P ++ P L+P +++ +LY +Y F + IPL+ V G+AFHPE+S+ + PY+EP F KI+ +L + A S ++ + + K + K LP ++ L + IL+ +E + S + W+LG FS DI LGV +NRL LG P+ L + R L QF + Q+RP F+
Sbjct: 41 AATKLQTNYYEFKTRELLVPK-------IEQDLFN----------QAPIEKQLTLQDNKQIISENEGGKLSNDLNE-----VATRLQANYRGFKTRKELKGQNIDKSNKEPVIGLLSESHDKDNITYEEGNKNISDELNEVATKLQANYCGFKIRKQLAYKNQGYSVNELVSEIQSGSQDNYIITSEEDKEINEAATKIQANFRGFKTRKQLAEKSEHGLFSQVPIEIPLILNYKDITISEVVRKTSSDDLEEDVTELEAHYRGYKTKKEFSLKNQ-------EDSLNEAATKTQS--RFYELNPNILE-----DGKETISDDVEEVNQTISEG--DNKTYSDDLEEVATRLQANYRGFKTRKELEAKTQEYSLNETREVQSRFHEVNQNISEGRNKTVSDYLNEAAIKIQSNYRGFKTRKEL------------------------EAKTQDDSLNEAATKIQANYLGFKTRRELIANIDYGLLIKTVTKTKLACYDKDQRISEMEGVNVPDDLNKSATKLQANYRGFKARKELATKPREDSLDETTSEIQSEFLELNHKFTEDKEKTIPDDFNESATKLQSNYRGFKIRKKC----NYEIVEKKDSNIKCDGVGLTNRKASNFEDIATRNYQPKNCELNLSTQDNANHRLDEFFVDPIEPSIVNDNVNYLSDELLQNFYKIRAATVIQAIFRGYLIRKKLAFGFGSLNSKIPDQKLINEQELLKINNAASVIQAYFKGKKSHDDYIKKQGSTNDFDSMTKYKQNANKATCAKDKSDSFEDAEFVIDSSNLEDDSTEYSERNFESKNSPDIKPLEDNINLVNNNYDERQTSEKDAAIKIQSHFRGFKERKTFKR-RKTFILQDSNHPLEESKVITDI-------------GAVPFSNTKSS---PQIDDVDGD-----LNAMNSVG-----------GKLYPYR----------------------------IRIFHYRESTESEKVLMYLCERGIEFSGYHMDLAENENYGDWFLKLNAKGEIPTMVFNETNIVSTAYNIIEFLESNIPVNSHPPPLIPCNTDPVLYPKYQEFIRKIGKIPLSIVSDGIAFHPEVSVLK----PYNEP-YFSKIRNDVRHLSTLLMEYASSVDDPSVQNALKSKADIHKSTLPRYTKIEEYKALLYSISLILDDIEKKFS--------LEPWVLGRKFSVADIYLGVLINRLILLG-NPKQILSN--RKKLPQFFRALKQRRPSFR 1145 HSP 2 Score: 160.614 bits (405), Expect = 9.752e-40 Identity = 201/821 (24.48%), Postives = 340/821 (41.41%), Query Frame = 0
Query: 280 VQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDP--------------EVEKAATKIQAGYKGMRVRREMRG---------PALALPSNSMDADDS---------SQEL--AATKIQAGYRGMRVRKGLKATQ---------AENEEG-----IDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSE-PMDI-------DLEDPEVERA---------ATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMR------ARRALRQHQEEIN--ADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVER----------------AATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSM-----------------------REKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGM--KP-----------VEAEPVPEDDTFDIDLEDP---EVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRR-------EMQGRNEIVEPAEDPIDIDLTDPEVD-------------RAATKIQAGYKGMRERKKLRE----------NQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNR---------NIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFP---EESPETGLTEDQAATKIQAGYRGMRARK 930
EAATK+Q + K R+ P+ + + +Q I + L D ++ + AT++QA Y+G + R+E++G P + L S S D D+ S EL ATK+QA Y G ++RK L +E + G I +D E+ AATKIQA ++G + RK+L + E S+ P++I D+ EV R T+++A Y+G + +K+ N+E+ NE AATK Q+ + + + + EE+N E + D D+E AT++QA Y+G + RKEL +E E ++ EV + AA KIQ+ Y+G ++RK + K +D + AA++IQA Y G +TR+ + ++ +E E ++ DLN K+AT++QA YRG + R+ + KP +++E + + F D E + +ATK+Q+ Y+G + R+ K + I D +A+ R + + + + + E DPI+ + + V+ RAAT IQA ++G RKKL +Q L + + + AA+ IQA ++G ++ DY+ ++ ++ ++ K N+ + ED ID S+ +E +T+ K ++ L + E +E AA KIQ+ +RG + RK
Sbjct: 38 FNEAATKLQTNYYEFKTRELLVPKIEQDLFNQAPIEK-QLTLQDNKQIISENEGGKLSNDLNEVATRLQANYRGFKTRKELKGQNIDKSNKEPVIGLLSESHDKDNITYEEGNKNISDELNEVATKLQANYCGFKIRKQLAYKNQGYSVNELVSEIQSGSQDNYIITSEEDKEINEAATKIQANFRGFKTRKQLAEKSEHGLFSQVPIEIPLILNYKDITISEVVRKTSSDDLEEDVTELEAHYRGYKTKKEFSLKNQEDSLNE-------------AATKTQSRFYELNPNILEDGKETISDDVEEVNQTISEGDNKTYSD-DLEEVATRLQANYRGFKTRKELEAKTQEYSLNETREVQSRFHEVNQNISEGRNKTVSDYLNEAAIKIQSNYRGFKTRKELEAKT-----------QDDSLNEAATKIQANYLGFKTRRELIANIDYGLLIKTVTKTKLACYDKDQRISEMEGVNVPDDLN-----KSATKLQANYRGFKARKELATKPREDSLDETTSEIQSEFLELNHKFTEDKEKTIPDDFNESATKLQSNYRGFKIRKKCNYEIVEKKDSNIKCDGVGLTNRKASNFEDIATRNYQPKNCELNLSTQDNANHRLDEFFVDPIEPSIVNDNVNYLSDELLQNFYKIRAATVIQAIFRGYLIRKKLAFGFGSLNSKIPDQKLINEQELLKINNAASVIQAYFKGKKSHDDYIKKQGSTNDFDSMTKYKQNANKATCAKDKSDSFEDAEFVIDSSN--LEDDSTEYSERNFESKNSPDIKPLEDNINLVNNNYDERQTSEKDAAIKIQSHFRGFKERK 825 HSP 3 Score: 155.221 bits (391), Expect = 3.946e-38 Identity = 214/836 (25.60%), Postives = 355/836 (42.46%), Query Frame = 0
Query: 368 AATKIQAGYRGMRVRKGL-----------------------KATQAENEEG-IDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPM-----------DIDLEDP------EVERAATKIQAGYKGMRARKQLKRDNEEEPANEVI---------DIDLT---DPEIEQAATKIQAGYKGMRARRALRQHQEE-INADEAIDIDL----------------EDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSR--KSMREKAAEHQDEIDI------------DLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDI--------DIDLNDPE---------VEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGL--------------RTSASIKAEDEIDIDMED---PDE-ERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPID----------IDL----------TDPE-VDRAATKIQAGYKGMRERKKLR----ENQALEIDLDDPDLA--QAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRN-IEDDIVDID---LSDPDVE-----KAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQ-------AATKIQAGYRGMRARKEIVPLNLSEMTRDGDP---FPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMRE-KAEEQPDAM----DIDLTDPEVER-------AATKIQAGYKGMRTRRELK 1026
AATK+Q Y + R+ L K +ENE G + DL++ AT++QA Y+G + RKEL+ + ++ EP+ +I E+ E+ ATK+QA Y G + RKQL N+ NE++ + +T D EI +AATKIQA ++G + R+ L + E + + I+I L D+E T+++A Y+G + +KE LK+ E + AATK Q+ + + + +E ++ +E++ DLE+ A+R+QA Y+G +TRK + K E + +++ N E + +AA +IQ+ YRG +TR+ E E +DD+ + AATKIQA Y G + RR L +T + +D+ +ME PD+ ++ATK+QA YRG + R+E+ + P ED +D ++L T P+ + +ATK+Q+ Y+G + RKK E + I D L +A+ R +Q + ++ +S + N LDEF + IE IV+ + LSD ++ +AAT IQA ++G RK L + P+ L +Q AA+ IQA ++G ++ + + S T D D + + D SD + + + T S R +++ PD +I+L + + AA KIQ+ ++G + R+ K
Sbjct: 41 AATKLQTNYYEFKTRELLVPKIEQDLFNQAPIEKQLTLQDNKQIISENEGGKLSNDLNE-----VATRLQANYRGFKTRKELKGQNIDKSNKEPVIGLLSESHDKDNITYEEGNKNISDELNEVATKLQANYCGFKIRKQLAYKNQGYSVNELVSEIQSGSQDNYIITSEEDKEINEAATKIQANFRGFKTRKQLAEKSEHGLFSQVPIEIPLILNYKDITISEVVRKTSSDDLEEDVTELEAHYRGYKTKKEF-----SLKNQE--------DSLNEAATKTQSRFYELNPNILEDGKETISDDVEEVNQTISEGDNKTYSDDLEE-----VATRLQANYRGFKTRKELEAKTQEYSLNETREVQSRFHEVNQNISEGRNKTVSDYLNEAAIKIQSNYRGFKTRK-----ELEAKTQDDSLN---------EAATKIQANYLGFKTRRELIANIDYGLLIKTVTKTKLACYDKDQRISEMEGVNVPDDLNKSATKLQANYRGFKARKELATK-----PREDSLDETTSEIQSEFLELNHKFTEDKEKTIPDDFNESATKLQSNYRGFKIRKKCNYEIVEKKDSNIKCDGVGLTNRKASNFEDIATRNYQPKNCELN----LSTQDNANHRLDEFFVDPIEPSIVNDNVNYLSDELLQNFYKIRAATVIQAIFRGYLIRKKLAFGFGSLNSKIPDQKLINEQELLKINNAASVIQAYFKGKKSHDDYIKKQGS--TNDFDSMTKYKQNANKATCAKDKSDSFEDAEFVIDSSNLEDDSTEYSERNFESKNSPDIKPLEDNINLVNNNYDERQTSEKDAAIKIQSHFRGFKERKTFK 828 HSP 4 Score: 146.747 bits (369), Expect = 1.944e-35 Identity = 202/809 (24.97%), Postives = 333/809 (41.16%), Query Frame = 0
Query: 95 DVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASP-------------------IEIDLADPEVEKAATKIQAGFKGMRTRKQM-RKAE-----------PVPEELADIDLDDP-------EMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKAR--KQAKPEAKDE-EDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSN-SMDADDSSQEL------------------AATKIQAGYRGMRVRKGLKA-TQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKE------------------LRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDT--EAIDIDLEDPEVERAATKIQAGY--KGMRSRKSMREKAAEHQDEIDIDLEDPEV------------------LMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDL-NDPEVEK---AATRIQAGYRGMRT------RQGM---------------KPVEAEPVP---EDDTFDID---LEDPEVERAATKIQAGYKGMRARRGLRTSASIK-AEDEIDIDMEDPDEER-----AATKIQAGYRGMRTRREMQGRNEIV 765
D+ AT++QA YRG K R+++K + D+S + I + ++ D D+ EE +I E EV ATK+QA Y G K RK L + I D E+ +AATKIQA F+G +TRKQ+ K+E P+ DI + + ++ + T+++A YRG +T++ + K ED ++ EAATK Q+ F + + K D+ E+ + I D ++E+ AT++QA Y+G + R+E+ N + + E+ AA KIQ+ YRG + RK L+A TQ D + AATKIQA Y G + R+E L + ++ SE +++ D ++ ++ATK+QA Y+G +ARK+L E+ +++ ++IQ+ + + + +E+ D D +ATK+Q+ Y+G + RK+ E KD+ + + L + + Y K S ++ A DE +D +P + + AA+ IQA ++G RK + D L N+ E+ K AA+ IQA ++G ++ +QG K A+ ED F ID LED E + ++ + S IK ED I++ + DE + AA KIQ+ +RG + R+ + R +
Sbjct: 96 DLNEVATRLQANYRGFKTRKELKGQNIDKSNKEPVIGLLSESHDK-------DNITYEEGNKNISDELNEV---ATKLQANYCGFKIRKQLAYKNQGYSVNELVSEIQSGSQDNYIITSEEDKEINEAATKIQANFRGFKTRKQLAEKSEHGLFSQVPIEIPLILNYKDITISEVVRKTSSDDLEEDVTELEAHYRGYKTKKEFSL--KNQEDSLN---------EAATKTQSRFYELNPNILEDGKETISDDVEEVNQTISEGDNKTYSDDLEEVATRLQANYRGFKTRKELEAKTQEYSLNETREVQSRFHEVNQNISEGRNKTVSDYLNEAAIKIQSNYRGFKTRKELEAKTQ------------DDSLNEAATKIQANYLGFKTRRELIANIDYGLLIKTVTKTKLACYDKDQRISEMEGVNVPD-DLNKSATKLQANYRGFKARKELATKPREDS-------------LDETTSEIQSEF--LELNHKFTEDKEKTIPD----------DFNESATKLQSNYRGFKIRKKCNYEIVEKKDSNIKCDGVGLTNRKASNFEDIATRNYQPKNCELNLSTQDNANHRLDEFFVDPIEPSIVNDNVNYLSDELLQNFYKIRAATVIQAIFRGYLIRKKLAFGFGSLNSKIPDQKLINEQELLKINNAASVIQAYFKGKKSHDDYIKKQGSTNDFDSMTKYKQNANKATCAKDKSDSFEDAEFVIDSSNLEDDSTEYSERNFES-----------KNSPDIKPLEDNINLVNNNYDERQTSEKDAAIKIQSHFRGFKERKTFKRRKTFI 834 HSP 5 Score: 124.02 bits (310), Expect = 1.688e-28 Identity = 139/569 (24.43%), Postives = 238/569 (41.83%), Query Frame = 0
Query: 815 DLAQAATKIQAGYRGHQARKDYVSRREV-VSNRKNIPKSLD-EFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTE---------------------DQAATKIQAGYRGMRARKEIVPLN----LSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE---------EQPDAMDI-DLTDPEVERA---------ATKIQAGYKGMRTRRELKIPYPEEAIHMA-----------DP-------------------------------EMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPD-MEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPED------------DETISAATKIQAGFKGMKARRDLSQLRQNRDLMDRDEDSQTVISDIQETLIVATHV---DALGAIPFDMHPSAV 1279
+ +AATK+Q Y + R+ V + E + N+ I K L + N+ I + LS+ D+ + AT++QA Y+G KTRK L+ + + P GL ++ ATK+QA Y G + RK++ N ++E+ + + I D E+ AATKIQA ++G +TRK + EK+E E P ++ D+T EV R T+++A Y+G +T++E + E++++ A +P ++E+ AT++QA YRG +TR+ ++ +E + E + + EG + T D + +AA KIQ+ +RG + RK ++ + D + AA+KIQA Y+G +TRR+L A +++ + + Y +D D+ +ATK+QA ++G KAR++L+ R++ S+IQ + H D IP D + SA
Sbjct: 37 NFNEAATKLQTNYYEFKTRELLVPKIEQDLFNQAPIEKQLTLQDNKQIISENEGGKLSN-DLNEVATRLQANYRGFKTRKELKGQNIDKSNKEPVIGLLSESHDKDNITYEEGNKNISDELNEVATKLQANYCGFKIRKQLAYKNQGYSVNELVSE---IQSGSQDNYIITSEEDKEINEAATKIQANFRGFKTRKQLAEKSEHGLFSQVPIEIPLILNYKDITISEVVRKTSSDDLEEDVTELEAHYRGYKTKKEFSLKNQEDSLNEAATKTQSRFYELNPNILEDGKETISDDVEEVNQTISEGDNKTYSDDLEEVATRLQANYRGFKTRKELE-------AKTQEYSLNETREVQSRFHEVNQNISEGR-------------------NKTVSDYLNEAAIKIQSNYRGFKTRKELEAKTQ-----------DDSLNEAATKIQANYLGFKTRRELIANIDYGLLIKTVTKTKLACYDKDQRISEMEGVNVPDDLNKSATKLQANYRGFKARKELAT-------KPREDSLDETTSEIQSEFLELNHKFTEDKEKTIPDDFNESAT 557
BLAST of sperm surface protein sp17 vs. L. salmonis genes
Match: EMLSAG00000003057 (supercontig:LSalAtl2s:LSalAtl2s172:803529:807762:-1 gene:EMLSAG00000003057 transcript:EMLSAT00000003057 description:"augustus_masked-LSalAtl2s172-processed-gene-7.0") HSP 1 Score: 168.703 bits (426), Expect = 4.129e-42 Identity = 162/578 (28.03%), Postives = 263/578 (45.50%), Query Frame = 0
Query: 159 DLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEI-DLADPEVEKAATKIQAGFKGMRTRKQMRK--AEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQ-HQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDID-------------LEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLM-AASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLR 718
DL D E+A AA KIQ+ +RG +TRK + + DL D E+ +AA KIQ+ F+G +TRK K EP+P +L+D + AA KIQ+ +RG ++R+ + D DL+D + EAA IQ+ F G AR KD + E++P DL D ++ ++A +IQ+ ++G + R+ + L + DD +AA KIQ+ +RG R+RK + + G +A +IQ ++G +ARK++R + + R+A KIQ+ YKG + RK L +N++ +AA IQ+ + + R+AL++ +Q I RA IQ+ +G ++RK L++ K+ + A+ I +E + E AA +IQ YKG ++RK D ++ E LM A +IQ+ Y+ + RK + E +Q KAA +IQ YR RT++ + ++ R+A IQ ++G +AR+ R
Sbjct: 9 DLNDKELAEAAVKIQSVFRGFQTRKDITKEDVESLPDLNDKELAEAAVKIQSVFRGFQTRKSAAKEDEEPLP------NLNDKGLADAAVKIQSVFRGFKSRKRV----NNINDHSLPDLNDENLSEAAIMIQSVFHGFHAR-------KDLNQADESLP----DLSDKDLAESAIRIQSVFRGFQTRKYITNNELP------NLDDKKLAMAALKIQSVFRGFRMRKKIDDKKTATLVG------------SAIRIQTVFRGYKARKKIRN----------------NLLLVRSAIKIQSHYKGYKVRKALINENKKR---------------MRAAILIQSCIRAYKVRKALKEDNQRRI----------------RAVILIQSCIRGYKSRKRLKEIKKT--TSAALMIQSCFKRFKKRKLALIEKQKEENAAIQIQKVYKGFKTRK---------------DKKEMETLMFAVIKIQSSYRSYKRRKEL-----EKDQ-------------KAALQIQRVYRCFRTKESTE--------------------DITRSAILIQGAFRGFKARKKAR 445 HSP 2 Score: 132.88 bits (333), Expect = 3.880e-31 Identity = 141/522 (27.01%), Postives = 228/522 (43.68%), Query Frame = 0
Query: 648 DLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEI--DLDDPDLAQAATKIQAGYRGHQARKDYVSRREV--VSNRK------NIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELK-IPYPEEAIHMADPEM----------------EQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARK 1142
DLND E+ +AA +IQ+ +RG +TR+ + + E +P DL D E+ AA KIQ+ ++G + R+ ++ K ++E ++ D AA KIQ+ +RG ++R+ + N+ P DL D + AA IQ+ + G RK L NQA E DL D DLA++A +IQ+ +RG Q RK Y++ E+ + ++K I F + D D + +A +IQ ++G K RK +R L ++A KIQ+ Y+G + RK ++ N M RAA IQ+ + + RK+++E + + RA IQ+ +G ++R+ LK I A M E AA +IQ Y+G +TR+ K ++E ++ I +S + E E D A A ++ + D+ ++A IQ FRG +ARK
Sbjct: 9 DLNDKELAEAAVKIQSVFRGFQTRKDITKEDVESLP-------DLNDKELAEAAVKIQSVFRGFQTRK-----SAAKEDEEPLPNLNDKGLADAAVKIQSVFRGFKSRKRVNNINDHSLP-------DLNDENLSEAAIMIQSVFHGFHARKDL--NQADESLPDLSDKDLAESAIRIQSVFRGFQTRK-YITNNELPNLDDKKLAMAALKIQSVFRGFRMRKKID----DKKTATLVGSAIRIQTVFRGYKARKKIR------------NNLLLVRSAIKIQSHYKGYKVRKALINENKKRM--------------------------RAAILIQSCIRAYKVRKALKEDNQRRI-------------RAVILIQSCIRGYKSRKRLKEIKKTTSAALMIQSCFKRFKKRKLALIEKQKEENAAIQIQKVYKGFKTRKDKK---EMETLMFAVIKIQSSYRSYKRRK-------ELEKDQKA-ALQIQRVYRCFRTKESTEDITRSAILIQGAFRGFKARK 442 HSP 3 Score: 128.642 bits (322), Expect = 6.628e-30 Identity = 139/538 (25.84%), Postives = 236/538 (43.87%), Query Frame = 0
Query: 90 DLNDPDVAVAATKIQAGYRGSKARRDMKSRRDDE----------SKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPE----VERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEV 613
DLND ++A AA KIQ+ +RG + R+ ++ D+E + ++KIQ + ++D DL D ++ AA IQ+ + G RK L A DL+D ++ ++A +IQ+ F+G +TRK + E +P +LDD ++ AA KIQ+ +RG + R+ K+ DK+ V +A +IQ FRG KARK+ + ++ + ++A KIQ+ YKG +VR+ ++ ++ + AA IQ+ R +VRK LK +N+ I RA IQ+ +G ++RK L+ E + AA IQ+ +K + RK + ++E E AA +IQ YKG + R+ ++ + + A KIQ+ Y+ + RKEL ++++ + + E + R+A IQ ++G ++RK R + + EI I + EV
Sbjct: 45 DLNDKELAEAAVKIQSVFRGFQTRKSA-AKEDEEPLPNLNDKGLADAAVKIQSVFRGFKSRKRVNNINDHSLP------DLNDENLSEAAIMIQSVFHGFHARKDLNQADESLPDLSDKDLAESAIRIQSVFRGFQTRKYITNNE-LP------NLDDKKLAMAALKIQSVFRGFRMRK--KIDDKKTATLVG----------SAIRIQTVFRGYKARKKIR--------------------NNLLLVRSAIKIQSHYKGYKVRK------------ALINENKKRMRAAILIQSCIRAYKVRKALK---EDNQRRI----------RAVILIQSCIRGYKSRKRLK----------------EIKKTTSAALMIQSCFKRFKKRKLALIEKQKE---------------ENAAIQIQKVYKGFKTRKDKKEME----------------TLMFAVIKIQSSYRSYKRRKELEKDQKAALQIQRVYRCFRTKESTEDITRSAILIQGAFRGFKARKKARLEREQILKEIRIVSKTKEV 464 HSP 4 Score: 110.538 bits (275), Expect = 2.259e-24 Identity = 119/439 (27.11%), Postives = 202/439 (46.01%), Query Frame = 0
Query: 810 DLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPD--VEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQ--AATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYP----------EDDETISAATKIQAGFKGMKARRDLSQL 1234
DL+D +LA+AA KIQ+ +RG Q RKD +++ +V S +P DL+D ++ +AA KIQ+ ++G +TRK+ EE + GL + AA KIQ+ +RG ++RK + +N + DL+D + AA IQ+ + G RK + + E PD D DL + +A +IQ+ ++G +TR+ + E ++ D ++ AA KIQ+ +RG R R K I D +VG I ++ G ++ +N L+ L +A + G + + + ++ AA IQ+ R + RK ++ ED N + A IQ+ G ++R+ L +I+ S +I + E + +AA +IQ +KG K R+D ++
Sbjct: 9 DLNDKELAEAAVKIQSVFRGFQTRKD-ITKEDVES----LP-----------------DLNDKELAEAAVKIQSVFRGFQTRKSAAKEDEEPLPNLNDKGLAD--AAVKIQSVFRGFKSRKRVNNINDHSLP-----------------DLNDENLSEAAIMIQSVFHGFHARKDLNQADESLPDLSDKDLAE-----SAIRIQSVFRGFQTRKYI---TNNELPNLDDKKLAMAALKIQSVFRGFRMR---KKIDDKKTATLVGSAIRIQTVFRGYKARKKIRNNLL---LVRSAIKIQSHYKGYKVRKALINENKKRMRAAILIQSCIRAYKVRKALK------ED------NQRRIRAVIL-IQSCIRGYKSRKRLK----EIKKTTSAALMIQSCFKRFKKRKLALIEKQKEENAAIQIQKVYKGFKTRKDKKEM 375 HSP 5 Score: 87.0409 bits (214), Expect = 3.698e-17 Identity = 77/291 (26.46%), Postives = 142/291 (48.80%), Query Frame = 0
Query: 959 DLSDPEVERAATKIQAGYKGMRTRKSM-REKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNE-EVIIGSYPEDDET------ISAATKIQAGFKGMKARRDLSQLRQNRDLM 1241
DL+D E+ AA KIQ+ ++G +TRK + +E E PD L D E+ AA KIQ+ ++G +TR+ E ++ D + AA KIQ+ +RG ++R+ + I D + ++ + SE + +++ + +++ E + D++ D+ ++A +IQ+ FRG Q RK I +L+D ++ AA KIQ+ + G R R+ + ++ + + + Y + + +A KIQ+ +KG K R+ L + +N+ M
Sbjct: 9 DLNDKELAEAAVKIQSVFRGFQTRKDITKEDVESLPD-----LNDKELAEAAVKIQSVFRGFQTRKSAAKEDEEPLPNLNDKGLADAAVKIQSVFRGFKSRKRVNNINDHSL---PDLNDENLSEAAI--------MIQSVFHGFHARKDLNQADESLP-DLSDKDLAESAIRIQSVFRGFQTRKYITNNELP-------NLDDKKLAMAALKIQSVFRGFRMRKKIDDKKTATLVGSAIRIQTVFRGYKARKKIRNNLLLVRSAIKIQSHYKGYKVRKAL--INENKKRM 273 HSP 6 Score: 84.7297 bits (208), Expect = 1.888e-16 Identity = 95/383 (24.80%), Postives = 172/383 (44.91%), Query Frame = 0
Query: 868 DLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTE-DQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAI-HMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINI-----DMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSY------PEDDE-TISAATKIQAGFKGMKARRDLSQLRQ 1236
DL+D ++ +AA KIQ+ ++G +TRK + +E E P+ E +AA KIQ+ +RG + RK S D +P P +L+D + AA KIQ+ ++G ++RK + D DL D + AA IQ+ + G R++L +E++ ++D ++ ++A +IQ+ +RG +TR+ + + +L +D + A +A ++ + + D + +A +IQ FRG +ARK I+ N+ + +A KIQ+ Y G + R+ L + I +Y ED++ I A IQ+ +G K+R+ L ++++
Sbjct: 9 DLNDKELAEAAVKIQSVFRGFQTRKDI---TKEDVESLPDLNDKELAEAAVKIQSVFRGFQTRK-------SAAKEDEEPLP----------NLNDKGLADAAVKIQSVFRGFKSRK----RVNNINDHSLPDLNDENLSEAAIMIQSVFHGFHARKDLN--QADESLPDLSDKDLAESAIRIQSVFRGFQTRKYI-------------------TNNELPNLDDKK--------LAMAALKIQSVFRGFRMRKKIDDKKTATLVGSAIRIQTVFRGYKARKKIR--------------NNLLLVRSAIKIQSHYKGYKVRKALINENKKRMRAAILIQSCIRAYKVRKALKEDNQRRIRAVILIQSCIRGYKSRKRLKEIKK 324 HSP 7 Score: 66.2402 bits (160), Expect = 6.949e-11 Identity = 53/161 (32.92%), Postives = 79/161 (49.07%), Query Frame = 0
Query: 421 ARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEE------LKDTEAIDIDLEDPEVER 575
A+KE+ + +D+E DLED +VE++A KIQA +KG + RK + D + E+E AA KIQ YKG +AR L++ E +M AA IQ+ YKG +ARK L ++ +E KD + I + D +E+
Sbjct: 641 AKKEM--SHKSSSDTEEELPDLEDSDVEKSAIKIQAVFKGFKTRKAIHNDKK---------------EMENAAIKIQCAYKGHKARENLQK---------------EKAEMVDAAIAIQSVYKGFKARKVLEESIKEKERVVQCKDAKKPSIVITDRSIEK 769 HSP 8 Score: 66.2402 bits (160), Expect = 7.435e-11 Identity = 42/118 (35.59%), Postives = 63/118 (53.39%), Query Frame = 0
Query: 525 DLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQ 642
DLED D+E++A KIQA +KG + RK + +K+E+ E AA KIQ YKG ++R++++++ AE D AA IQ+ YKG + RK + E E E+
Sbjct: 659 DLEDSDVEKSAIKIQAVFKGFKTRKAIHNDKKEM---------------ENAAIKIQCAYKGHKARENLQKEKAEMVD-------------AAIAIQSVYKGFKARKVLEESIKEKER 748 HSP 9 Score: 62.003 bits (149), Expect = 1.390e-9 Identity = 41/111 (36.94%), Postives = 60/111 (54.05%), Query Frame = 0
Query: 399 DLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRAL 509
DL+D +VE++A KIQA +KG + RK + ND + E+E AA KIQ YKG +AR+ L+++ E++D AA IQ+ YKG +AR+ L
Sbjct: 659 DLEDSDVEKSAIKIQAVFKGFKTRKAIH------NDKK---------EMENAAIKIQCAYKGHKARENLQKEKA-----EMVD----------AAIAIQSVYKGFKARKVL 739 HSP 10 Score: 61.2326 bits (147), Expect = 2.799e-9 Identity = 58/183 (31.69%), Postives = 87/183 (47.54%), Query Frame = 0
Query: 194 DLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEE------DSQENIPSIDID---------LDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDAD 361
DL D +VEK+A KIQA FKG +TRK I D EM AA KIQ Y+G + R ++ K+ + VD AA IQ+ ++G KARK + K++E D+++ PSI I +D+ ++ A K K + ++ E + P P++S+D D
Sbjct: 659 DLEDSDVEKSAIKIQAVFKGFKTRKA-------------IHNDKKEMENAAIKIQCAYKGHKARENLQ---KEKAEMVD----------AAIAIQSVYKGFKARKVLEESIKEKERVVQCKDAKK--PSIVITDRSIEKNDAIDEKQIRSALKKGGIPAKDITIKGEYKEP--VXPASSIDFD 811 HSP 11 Score: 56.225 bits (134), Expect = 8.041e-8 Identity = 42/132 (31.82%), Postives = 65/132 (49.24%), Query Frame = 0
Query: 589 SRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTS 720
++K M K++ +E DLED +V +A +IQA +KG +TRK+ I + E+E AA +IQ Y+G + R+ ++ +AE V AA IQ+ YKG +AR+ L S
Sbjct: 641 AKKEMSHKSSSDTEEELPDLEDSDVEKSAIKIQAVFKGFKTRKA--------------IHNDKKEMENAAIKIQCAYKGHKARENLQKEKAEMVD----------------AAIAIQSVYKGFKARKVLEES 742 HSP 12 Score: 56.225 bits (134), Expect = 8.317e-8 Identity = 41/125 (32.80%), Postives = 59/125 (47.20%), Query Frame = 0
Query: 868 DLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQ 992
DL D DVEK+A KIQA +KG KTRK + +E + AA KIQ Y+G +AR+ + ++ + E +D AA IQ+ YKG + RK + E +E+
Sbjct: 659 DLEDSDVEKSAIKIQAVFKGFKTRKAIHNDKKEM-----------ENAAIKIQCAYKGHKARENL----------------QKEKAEMVD----------AAIAIQSVYKGFKARKVLEESIKEK 746 HSP 13 Score: 56.225 bits (134), Expect = 9.125e-8 Identity = 59/212 (27.83%), Postives = 95/212 (44.81%), Query Frame = 0
Query: 1036 MADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPE-DDETIS-AATKIQAGFKGMKARRDLSQLRQNR-DLMDRD 1244
+ D E+ +AA KIQ+ +RG +TR+ D++K D V++ D+ ++ +AA KIQ+ FRG Q RK AK ++ +LND + AA KIQ+ + G ++R+ + + S P+ +DE +S AA IQ+ F G AR+DL+Q ++ DL D+D
Sbjct: 10 LNDKELAEAAVKIQSVFRGFQTRK------------------------DITKED------------------VESLP-----DLNDKELAEAAVKIQSVFRGFQTRKS----AAKEDEEPLPNLNDKGLADAAVKIQSVFRGFKSRKRV-------------NNINDHSLPDLNDENLSEAAIMIQSVFHGFHARKDLNQADESLPDLSDKD 157 HSP 14 Score: 55.0694 bits (131), Expect = 2.176e-7 Identity = 33/82 (40.24%), Postives = 45/82 (54.88%), Query Frame = 0
Query: 981 TRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMK 1062
+ KS + EE PD L D +VE++A KIQA +KG +TR+ AIH EME AA KIQ Y+G + R ++
Sbjct: 646 SHKSSSDTEEELPD-----LEDSDVEKSAIKIQAVFKGFKTRK---------AIHNDKKEMENAAIKIQCAYKGHKARENLQ 713
BLAST of sperm surface protein sp17 vs. L. salmonis genes
Match: EMLSAG00000008417 (supercontig:LSalAtl2s:LSalAtl2s5158:2614:5490:1 gene:EMLSAG00000008417 transcript:EMLSAT00000008417 description:"snap_masked-LSalAtl2s5158-processed-gene-0.4") HSP 1 Score: 89.7373 bits (221), Expect = 3.766e-18 Identity = 78/231 (33.77%), Postives = 115/231 (49.78%), Query Frame = 0
Query: 95 DVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPED---------------EVDIDLDDP-----EVQEAATKIQAGFRGMKARKQAKPEAK 305
D+ AATKIQA Y G K R++ +++ D+ + VQ++ +E + + +D E ++ DLE+ ATKIQA Y G K RK L A E L D AAT+IQ+ F + T+ EP P++L +A TK+QA YRG +TR+ ++ K+ ED +I DD ++ EAATK+QA FRG + RKQ ++K
Sbjct: 520 DLNKAATKIQANYCGFKTRKEFEAKAQDDLLNHAATE--VQSIFNESDAMISEDNERLVSD---DLEE-----VATKIQANYCGFKIRKEL-TAKTQEDSLND-----AATEIQSIFHKVNTKIPKDSEEPFPDDLE----------EAVTKLQAHYRGYKTRK--EITSKKKEDLNNAATNTQSEFHKLNQNISEDDKKSISEDLNEAATKLQANFRGFRTRKQLAAKSK 722 HSP 2 Score: 85.5001 bits (210), Expect = 7.041e-17 Identity = 74/251 (29.48%), Postives = 116/251 (46.22%), Query Frame = 0
Query: 404 EVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALR-QHQEEINADEAIDI-------------DLEDP---DMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKA 637
++ +AATKIQA Y G + RKE + +D + AAT++Q+ + A + DNE L ++E+ ATKIQA Y G + R+ L + QE+ D A +I D E+P D+E A TK+QA Y+G + RKE+ K+E ++ AAT Q+ + + S +K + I DL + AA+++QA ++G RTRK + K+
Sbjct: 520 DLNKAATKIQANYCGFKTRKEF-------------EAKAQDDLLNHAATEVQSIFNESDA--MISEDNER----------LVSDDLEEVATKIQANYCGFKIRKELTAKTQEDSLNDAATEIQSIFHKVNTKIPKDSEEPFPDDLEEAVTKLQAHYRGYKTRKEITSKKKE--------------DLNNAATNTQSEFHKLNQNISEDDKKS-----ISEDLNE-----AATKLQANFRGFRTRKQLAAKS 721 HSP 3 Score: 83.1889 bits (204), Expect = 3.654e-16 Identity = 102/409 (24.94%), Postives = 178/409 (43.52%), Query Frame = 0
Query: 95 DVAVAATKIQAGYRGSKARRDMKSRRD----------DESKQSIKIQMPV---QTMDDEDEGLELDDPEA---EEAELDIDLEDPEVANAATKI-QAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDP---EVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLE----------------DPEVERAATKIQAGYKGMRARKQL 467
D + AT IQA ++G K + D+ ++ DE+ ++K+ + Q + + + G + + E+ + D +E+ E +A Y G T+K + + + KAA++IQ F + ++ K E L D +N+AATKIQA Y G +TR+ E + D + AAT++Q+ F + +A ED++ L ++E+ ATKIQA Y G ++R+E+ +DS + AAT+IQ+ + + + I D ++P ++E A TK+QA Y+G + RKE+ ++E ++ + E ++ AATK+QA ++G R RKQL
Sbjct: 375 DRQIKATIIQAYFKGYKTKTDISNKVKKALQNKITEYDENNTNMKVNNEINYEQDIFNTNYGKACINKHSLLEEKTKDDKKIEETEEKTIQNDFNEAYYNGFNTKKEFKXKIEED------SLNKAASEIQLRFHNLD--QKCFKVEG-GNNLGD-------LNKAATKIQANYCGFKTRK-----------EFEAKAQDDLLNHAATEVQSIFN--------ESDAMISEDNER--------LVSDDLEEVATKIQANYCGFKIRKELTAKT---------QEDSLND-AATEIQSIFHKVNTK-------------IPKDSEEPFPDDLEEAVTKLQAHYRGYKTRKEITSKKKEDLNNAATNTQSEFHKLNQNISEDDKKSISEDLNEAATKLQANFRGFRTRKQL 717 HSP 4 Score: 80.4925 bits (197), Expect = 2.365e-15 Identity = 64/226 (28.32%), Postives = 102/226 (45.13%), Query Frame = 0
Query: 964 EVERAATKIQAGYKGMRTRKSMREKAEE---------------QPDAMDID----LTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPE-----------------------MEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGR 1147
++ +AATKIQA Y G +TRK KA++ + DAM + L ++E ATKIQA Y G + R+EL E++++ A E +E+A TK+QA YRG +TR+ EIT + + + + + + Q+E +L+ S + + E D+ +AATK+QA FRG + RK + +
Sbjct: 520 DLNKAATKIQANYCGFKTRKEFEAKAQDDLLNHAATEVQSIFNESDAMISEDNERLVSDDLEEVATKIQANYCGFKIRKELTAKTQEDSLNDAATEIQSIFHKVNTKIPKDSEEPFPDDLEEAVTKLQAHYRGYKTRK--------------EITSKKKEDLNNAATNTQSEF--HKLNQNISEDDKKSISE---------DLNEAATKLQANFRGFRTRKQLAAK 720 HSP 5 Score: 77.411 bits (189), Expect = 2.077e-14 Identity = 68/226 (30.09%), Postives = 106/226 (46.90%), Query Frame = 0
Query: 572 EVERAATKIQAGYKGMRSRKSMREKAAE----HQ--------DEIDIDL-EDPEVLMA------ASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRT-------SASIKAEDEI-----DIDMEDP-----DEERAATKIQAGYRGMRTRREMQGR 761
++ +AATKIQA Y G ++RK KA + H +E D + ED E L++ A++IQA Y G + RK + K E LND AAT IQ+ + + T+ + EP P+D +E A TK+QA Y+G + R+ + + +A+ + E +I +D D AATK+QA +RG RTR+++ +
Sbjct: 520 DLNKAATKIQANYCGFKTRKEFEAKAQDDLLNHAATEVQSIFNESDAMISEDNERLVSDDLEEVATKIQANYCGFKIRKELTAKTQEDS-------LND-----AATEIQSIFHKVNTK--IPKDSEEPFPDD-----------LEEAVTKLQAHYRGYKTRKEITSKKKEDLNNAATNTQSEFHKLNQNISEDDKKSISEDLNEAATKLQANFRGFRTRKQLAAK 720 HSP 6 Score: 76.2554 bits (186), Expect = 5.394e-14 Identity = 76/247 (30.77%), Postives = 124/247 (50.20%), Query Frame = 0
Query: 781 VDRAATKIQAGYKGMRERK-KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRRE--VVSNRKNIPKSLDEFNRNIEDDIVDID---LSDPDVEKAATKIQAGYKGMKTRKTLRALPEE--------------------FPEESPETGLTE-DQAATKIQAGYRGMRARKEIVP-----LNLSEMTRDGDPFPE------EGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKA 989
+++AA++IQ + + ++ K+ L DL +AATKIQA Y G + RK++ ++ + ++++ +S+ FN + D ++ D L D+E+ ATKIQA Y G K RK L A +E P++S E + ++A TK+QA YRG + RKEI LN + T F + E + ++I DL++ AATK+QA ++G RTRK + K+
Sbjct: 491 LNKAASEIQLRFHNLDQKCFKVEGGNNL------GDLNKAATKIQANYCGFKTRKEFEAKAQDDLLNHAATEVQSI--FNES--DAMISEDNERLVSDDLEEVATKIQANYCGFKIRKELTAKTQEDSLNDAATEIQSIFHKVNTKIPKDSEEPFPDDLEEAVTKLQAHYRGYKTRKEITSKKKEDLN-NAATNTQSEFHKLNQNISEDDKKSISEDLNE-----AATKLQANFRGFRTRKQLAAKS 721 HSP 7 Score: 75.0998 bits (183), Expect = 1.330e-13 Identity = 101/396 (25.51%), Postives = 169/396 (42.68%), Query Frame = 0
Query: 878 ATKIQAGYKGMKTR-----KTLRALPEEFPE-ESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAA-----EVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKG----------------IQGRVAKAEDAFDIDLNDP---EVEAAASKIQAGYMGMRTRRDLAARRP-DIETVESNEEV----IIGSYPEDDE-TIS-----AATKIQAGFKGMKARRDLS 1232
AT IQA +KG KT+ K +AL + E + T + + Q + + I +L E D EE E + I D + +A Y G T+K + K + D+++ +AA++IQ + + + K E ++ D + +AATKIQA Y G +TR+ + ++++ DL L+HAA+ E DA N + + D+E+ ATKIQA + G + RK IQ K D +P ++E A +K+QA Y G +TR+++ +++ D+ +N + + + EDD+ +IS AATK+QA F+G + R+ L+
Sbjct: 380 ATIIQAYFKGYKTKTDISNKVKKALQNKITEYDENNTNMKVNNEINYEQDIFNTNYGKACINKHSLLEEKTKDDKKIEETEEKTIQNDFN-----------EAYYNGFNTKKEFKXKI--EEDSLN---------KAASEIQLRFHNL----DQKCFKVEGGNNLGD--LNKAATKIQANYCGFKTRKEFE----------------AKAQDDL-------------LNHAATEVQSIFNESDAMISEDNERLVSDDLEEVATKIQANYCGFKIRKELTAKTQEDSLNDAATEIQSIFHKVNTKIPKDSEEPFPDDLEEAVTKLQAHYRGYKTRKEITSKKKEDLNNAATNTQSEFHKLNQNISEDDKKSISEDLNEAATKLQANFRGFRTRKQLA 718 HSP 8 Score: 73.1738 bits (178), Expect = 4.495e-13 Identity = 63/198 (31.82%), Postives = 89/198 (44.95%), Query Frame = 0
Query: 1 MTQKPLFIRLNLPTGLEESLEGLAKEVLRSQPSNILHFSAEYFENQGLPANQDIFDYIALSSDVNPNKYDNAPPLEDGTEVENLSGDDIDLNDPD-----------VAVAATKIQAGYRG--SKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHL 185
M+ +PLFIRL LP+GLE+ LEGLAKEVLR +PS+I FSA +FE+ L + +Y P+ N LED G+ D+ P AAT+IQ+G++G S+ R+ + S + E +SI I AA+ IQA ++G TR+H
Sbjct: 1 MSDRPLFIRLTLPSGLEDILEGLAKEVLRHKPSDIYEFSALHFEDL-LRRRDGLCEY--------PHSSTNECTLEDN------CGNLSDIERPKTPNPENNHIRPTENAATQIQSGFKGMSSRGRKVIDSEKRQE--KSIGINC-----------------------------------AASLIQAVFKGYLTRQHF 146 HSP 9 Score: 63.929 bits (154), Expect = 3.510e-10 Identity = 54/185 (29.19%), Postives = 85/185 (45.95%), Query Frame = 0
Query: 84 LSGDDIDLNDPDVAVAATKIQAGYRGSKARRDM--KSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQ 266
+S D+ L D+ ATKIQA Y G K R+++ K++ D + + +IQ ++ + P+ E DLE+ A TK+QA YRG KTRK + ++ AAT Q+ F + + + E+L N+AATK+QA +RG +TR+ + K
Sbjct: 568 ISEDNERLVSDDLEEVATKIQANYCGFKIRKELTAKTQEDSLNDAATEIQSIFHKVNTK-------IPKDSEEPFPDDLEE-----AVTKLQAHYRGYKTRKEITSKKK-------EDLNNAATNTQSEFHKLNQNISEDDKKSISEDL----------NEAATKLQANFRGFRTRKQLAAKSKH 723
BLAST of sperm surface protein sp17 vs. L. salmonis genes
Match: EMLSAG00000004336 (supercontig:LSalAtl2s:LSalAtl2s2288:206:7966:1 gene:EMLSAG00000004336 transcript:EMLSAT00000004336 description:"maker-LSalAtl2s2288-snap-gene-0.4") HSP 1 Score: 79.7221 bits (195), Expect = 6.775e-15 Identity = 116/416 (27.88%), Postives = 179/416 (43.03%), Query Frame = 0
Query: 171 KIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMR-----KAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDN---EEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRAR------RALRQHQE-----------EINADEAIDIDLEDP-DMERAATKIQAGYKGMRARKE--LRQNKEEL 558
KIQ Y+G ++K D E AATKIQA F G + R + R +A+ V E + DL D TKIQA ++ + +K ++ ++++D + AATKIQA FRG++ARK + I + + E A K + KG EM + L S D + LAATKIQA +RG +VRK ++ +A ++ AA+KIQ ++G +AR E+ + N+ AA++I+ + G +RK+++ N EEE LTD KIQ +KG + + + Q + E+N + I I E+ D AA IQ+ KG RKE ++ +KE++
Sbjct: 14 KIQVPYKGYISKKGE--------DKNWEESNIAATKIQAHFMGGKARNEFRHLYKREAKLVNESKNNEDLTD------LTKIQAQFKDKLAIKDVK--------QLKVNINDETI--AATKIQAQFRGIQARKDVQK----------------IKVRNKEETNGAIKAISQLKG-----EMESVEVKLLKTSKD----DEILAATKIQAQFRGTQVRKDIEGIKASKQKDT----------IAASKIQEQFRGEKARNEVNVLKASENNK------------IHAASQIKVQFTG--SRKEVQEINMRKEEET--------LTDK-------KIQELFKGTNTQKEEEFMKTIAQQETNTATKCPSQIFEMNEQKEIAIIKENKQDEILAAMIIQSLIKGNLVRKEAKIKMHKEKI 341 HSP 2 Score: 78.9518 bits (193), Expect = 1.085e-14 Identity = 98/336 (29.17%), Postives = 156/336 (46.43%), Query Frame = 0
Query: 703 KIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEER---AATKIQAGYRGMRTRREMQG--RNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIP---KSLDEFNRNIEDDIVDIDL---SDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMRE---KAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRE 1024
KIQ YKG +++G ED + E AATKIQA + G + R E + + E E + DLTD TKIQA +K ++ +++ + L+++++D +A ATKIQA +RG QARKD ++ V N++ K++ + +E V++ L S D AATKIQA ++G + RK + + +++ AA+KIQ +RG +AR E+ L SE + AA++I+ + G +RK ++E + EE+ LTD KIQ +KG T++E
Sbjct: 14 KIQVPYKGYISKKG-----------------EDKNWEESNIAATKIQAHFMGGKARNEFRHLYKREAKLVNESKNNEDLTD------LTKIQAQFK---DKLAIKDVKQLKVNINDETIA--ATKIQAQFRGIQARKDV--QKIKVRNKEETNGAIKAISQLKGEMES--VEVKLLKTSKDDEILAATKIQAQFRGTQVRKDIEGIKASKQKDTI--------AASKIQEQFRGEKARNEVNVLKASENNKI-----------------------HAASQIKVQFTG--SRKEVQEINMRKEEET------LTDK-------KIQELFKGTNTQKE 271 HSP 3 Score: 78.5666 bits (192), Expect = 1.351e-14 Identity = 101/332 (30.42%), Postives = 154/332 (46.39%), Query Frame = 0
Query: 411 KIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQL----KRDNE---EEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQ----NKEE----------LK-DTEAIDIDL----EDPEVERAATKIQAGYKGMRSRKSMRE-KAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARR 715
KIQ YKG ++K ++ EE N AATKIQA + G +AR + KR+ + E NE DLTD TKIQA +K A + ++Q + IN DE I AATKIQA ++G++ARK++++ NKEE LK + E++++ L +D E+ AATKIQA ++G + RK + KA++ +D I AAS+IQ ++G + R + A +E I AA++I+ + G R K V+ +I++ E KIQ +KG ++
Sbjct: 14 KIQVPYKGYISKKGEDKNWEESN---------------IAATKIQAHFMGGKARNEFRHLYKREAKLVNESKNNE----DLTD------LTKIQAQFKDKLAIKDVKQLKVNIN-DETI-----------AATKIQAQFRGIQARKDVQKIKVRNKEETNGAIKAISQLKGEMESVEVKLLKTSKDDEI-LAATKIQAQFRGTQVRKDIEGIKASKQKDTI-----------AASKIQEQFRGEKARNEVNVLKA-SENNKI----------HAASQIKVQFTGSR-----KEVQ----------EINMRKEEETLTDKKIQELFKGTNTQK 270 HSP 4 Score: 67.781 bits (164), Expect = 2.810e-11 Identity = 66/216 (30.56%), Postives = 105/216 (48.61%), Query Frame = 0
Query: 98 VAATKIQAGYRGSKARRDMK------SRRDDESKQS------IKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMK-VHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQA 300
+AATKIQA + G KAR + + ++ +ESK + KIQ + + L + D + +L +++ D + AATKIQA +RG++ RK ++ +I + + E A K + KG + E V +L DD + AATKIQA +RG Q R+ ++ + + +D + AA+KIQ FRG KAR +
Sbjct: 37 IAATKIQAHFMGGKARNEFRHLYKREAKLVNESKNNEDLTDLTKIQAQFK------DKLAIKDVK----QLKVNINDETI--AATKIQAQFRGIQARKDVQ-----KIKVRNKEETNGAIKAISQLKG--------EMESVEVKLLKTSKDDEIL--AATKIQAQFRGTQVRKDIEGIKASKQKDTI-----------AASKIQEQFRGEKARNEV 214 HSP 5 Score: 67.0106 bits (162), Expect = 4.694e-11 Identity = 99/399 (24.81%), Postives = 164/399 (41.10%), Query Frame = 0
Query: 537 KIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPL 935
KIQ YKG ++K +N E E AATKIQA + G ++R R ++ + ++ E L ++IQA +K +K A + + +++ND + AAT+IQA +RG++ R+ ++ ++ R + K + +G S +K + DE AATKIQA +RG + R++++G +D I AA+KIQ ++G + R ++ +A E + AA++I+ + G SR+EV E N E++ L+D KIQ +KG T+K EEF + + E ATK + M +KEI +
Sbjct: 14 KIQVPYKGYISKKGEDKNWE---------------ESNIAATKIQAHFMGGKARNEFRHLYKREAKLVN-ESKNNEDLTDLTKIQAQFK---------DKLAIKDVKQLKVNINDETI--AATKIQAQFRGIQARKDVQKIKV-------------------RNKEETNGAIKAISQLKGEMESVEVKL-----LKTSKDDEILAATKIQAQFRGTQVRKDIEGIK--ASKQKDTI-----------AASKIQEQFRGEKARNEVNVLKASE-----NNKIHAASQIKVQFTG--------SRKEV-----------QEINMRKEEET----LTDK-------KIQELFKGTNTQK-----EEEFMKTIAQ---QETNTATKCPSQIFEMNEQKEIAII 305 HSP 6 Score: 63.929 bits (154), Expect = 4.627e-10 Identity = 81/309 (26.21%), Postives = 130/309 (42.07%), Query Frame = 0
Query: 971 KIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEA-----------------------------------IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDME-QAATKIQAGFRGMQARKGIQG-RVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDL------------AARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRD 1230
KIQ YKG ++K + EE AATKIQA + G + R E + Y EA +++ D + AATKIQA +RG++ R+ ++ I ++ + E+ G + + ++GE++ + EV T+ D E AATKIQA FRG Q RK I+G + +K +D AASKIQ + G + R ++ AA + ++ S +EV + +++ET++ KIQ FKG +++
Sbjct: 14 KIQVPYKGYISKKGEDKNWEES-------------NIAATKIQAHFMGGKARNEFRHLYKREAKLVNESKNNEDLTDLTKIQAQFKDKLAIKDVKQLKVNINDETI--AATKIQAQFRGIQARKDVQKI---------KVRNKEETNGAIKAISQ----LKGEME----SVEVKLLK-------TSKDDEILAATKIQAQFRGTQVRKDIEGIKASKQKDTI-----------AASKIQEQFRGEKARNEVNVLKASENNKIHAASQIKVQFTGSRKEVQEINMRKEEETLTDK-KIQELFKGTNTQKE 271 HSP 7 Score: 62.7734 bits (151), Expect = 8.551e-10 Identity = 82/309 (26.54%), Postives = 145/309 (46.93%), Query Frame = 0
Query: 89 IDLNDPDVAVAATKIQAGYRGSKARRD---MKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQ--AATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQ---AKPEAKDEEDSQENIPSIDIDLDDPE---VEK--------AATKIQAGYKGMRVRRE----MRGPALALPSNSMDADDSSQELAATKIQA 374
+ +N D +AATKIQA +RG +AR+D +K R +E+ +IK + + E E +E+ +L +D E+ AATKIQA +RG + RK + I + + AA+KIQ F+G + R ++ L E N+ AA++I+ + G +R+ ++ + + E+E L D ++QE F+G +K+ K A+ E ++ PS ++++ + + K AA IQ+ KG VR+E M + +N + ++S+ L + + A
Sbjct: 97 LKVNINDETIAATKIQAQFRGIQARKDVQKIKVRNKEETNGAIK---AISQLKGEMESVEV--------KLLKTSKDDEIL-AATKIQAQFRGTQVRKDIEG-----IKASKQKDTIAASKIQEQFRGEKARNEVNV------------LKASENNKIHAASQIKVQFTG--SRKEVQEINMRKEEET---LTDKKIQEL-------FKGTNTQKEEEFMKTIAQQETNTATKCPSQIFEMNEQKEIAIIKENKQDEILAAMIIQSLIKGNLVRKEAKIKMHKEKITFSTNQNEVENSNGLLTSCDVNA 364 HSP 8 Score: 62.003 bits (149), Expect = 1.839e-9 Identity = 74/245 (30.20%), Postives = 106/245 (43.27%), Query Frame = 0
Query: 1011 KIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQ----------GRVAKAEDAFDIDLNDPEVE----------AAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQLR 1235
KIQ YKG +++ + E I AATKIQA + G + R + + E + E + +E DL+K+ Q A D +NI+ D AATKIQA FRG+QARK +Q KA ++ EV+ AA+KIQA + G + R+D+ E I S +D TI AA+KIQ F+G KAR +++ L+
Sbjct: 14 KIQVPYKGYISKKGEDKNWEESNI---------AATKIQAHFMGGKARNEFRHLYKREAKLVNE-SKNNEDLTDLTKIQAQ---------FKDKLAIKDVKQLKVNIN----DETIAATKIQAQFRGIQARKDVQKIKVRNKEETNGAIKAISQLKGEMESVEVKLLKTSKDDEILAATKIQAQFRGTQVRKDI--------------EGIKASKQKD--TI-AASKIQEQFRGEKARNEVNVLK 218
BLAST of sperm surface protein sp17 vs. L. salmonis genes
Match: EMLSAG00000001051 (supercontig:LSalAtl2s:LSalAtl2s1172:54317:57440:-1 gene:EMLSAG00000001051 transcript:EMLSAT00000001051 description:"maker-LSalAtl2s1172-snap-gene-0.47") HSP 1 Score: 76.6406 bits (187), Expect = 2.526e-14 Identity = 83/273 (30.40%), Postives = 117/273 (42.86%), Query Frame = 0
Query: 1350 MLYHYSRSYNSQKVVIYLRERGIQYSGYH-VDLQRNEHLSNWFLDLNPKGEVPVMKF----DNQ-VIVDSSRIIHHLEAHVPGETYPLLVPLSSETLLYQQYLYFAALLDNIPLNQVVQGVAFHPEL-SLAQGRSEPYDEPQVFEKIQQTAIRRGLVLRQTAESNEKAASDLRKRASQFEKEILPNVKDRSAFEDTLDQVESILEKVEAQLSTTMHLEDECQGWLLGSSFSALDITLGVSLNRLAQLGLQPRFWLGDPARPHLLQFLEEIQKR 1615
+LY++S S S+KV I + E+ I + VD+ E +WFLDLNPKGEVPV + DN+ VI +S+ I+ +++ + L + E Q L F LD+ N + + EL SL Q SE FEKEI + +VE +L+++EA LS ED +L G F +DI+L L RL QLGL FW GD R ++L + I KR
Sbjct: 329 ILYYHSISSPSRKVRITMFEKNISSIEFKSVDIIDGEQFEDWFLDLNPKGEVPVFVYYDEEDNKTVITESTEIMKFIDSKYNSKNR--LFTYTDEEKDLQGMLDF---LDHSSYNALRDKASKLIELNSLFQNVSE------------------------------------------FEKEI-------------MFKVEVLLDRIEAILSN----EDRIGPYLFGPVFRGVDISLSALLFRLHQLGLDEYFW-GDGNRANILMYYGLIMKR 536
BLAST of sperm surface protein sp17 vs. L. salmonis genes
Match: EMLSAG00000003736 (supercontig:LSalAtl2s:LSalAtl2s2008:13405:23287:1 gene:EMLSAG00000003736 transcript:EMLSAT00000003736 description:"maker-LSalAtl2s2008-snap-gene-0.5") HSP 1 Score: 74.3294 bits (181), Expect = 2.457e-13 Identity = 62/153 (40.52%), Postives = 83/153 (54.25%), Query Frame = 0
Query: 268 EDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAG-YRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGM 419
EDE D+D E+ EAA +IQ+ FRG+K +K +DS DLD EV +AA KIQ+ YKG++ R+ P LP SMDA D ++ AA +IQ YRG + RK LK EN + I D + ++ RAA IQ Y+G
Sbjct: 783 EDESFADMDAEELAEAAMQIQSVFRGIKDKK---------DDSH--------DLDPVEVIRAALKIQSVYKGVKSRKLNNKPT-PLPQESMDAGDVAE--AALRIQYPVYRGFKARKELK---KENTQ-IKKDEEQYQLSRAAVTIQTIYRGF 911 HSP 2 Score: 63.5438 bits (153), Expect = 4.896e-10 Identity = 49/148 (33.11%), Postives = 80/148 (54.05%), Query Frame = 0
Query: 151 AEEAELDIDLEDPEVANAATKIQAGYRGMKTRK---HLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAG-YRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGM 294
+E E D++ E+A AA +IQ+ +RG+K +K H DL EV +AA KIQ+ +KG+++RK K P+P+E +D ++ +AA +IQ YRG + R+ +K + Q + D + ++ AA IQ +RG
Sbjct: 781 TQEDESFADMDAEELAEAAMQIQSVFRGIKDKKDDSH---------DLDPVEVIRAALKIQSVYKGVKSRKLNNKPTPLPQE----SMDAGDVAEAALRIQYPVYRGFKARKELKKENTQ----IKKDEEQYQLSRAAVTIQTIYRGF 911 HSP 3 Score: 58.9214 bits (141), Expect = 1.289e-8 Identity = 50/149 (33.56%), Postives = 80/149 (53.69%), Query Frame = 0
Query: 399 DLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPA-NEVIDIDLTDPEIEQAATKIQAG-YKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGM 545
D+D E+ AA +IQ+ ++G++ +K+ DS DL+ EV RAA KIQ+ YKG+++RK +N+ P E +D ++ +AA +IQ Y+G +AR+ L++ +I DE E + RAA IQ Y+G
Sbjct: 789 DMDAEELAEAAMQIQSVFRGIKDKKD---------DSH----DLDPVEVIRAALKIQSVYKGVKSRKL---NNKPTPLPQESMDAG----DVAEAALRIQYPVYRGFKARKELKKENTQIKKDE------EQYQLSRAAVTIQTIYRGF 911 HSP 4 Score: 53.9138 bits (128), Expect = 4.259e-7 Identity = 48/155 (30.97%), Postives = 80/155 (51.61%), Query Frame = 0
Query: 600 HQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ-GMKPVEAEPVPEDDTFDIDLEDPEVERAATKIQAG-YKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGM 752
+DE D++ E+ AA +IQ+ ++G++ +K D DL+ EV +AA +IQ+ Y+G+++R+ KP P+P++ ++ +V AA +IQ Y+G +AR+ L+ K +I D E RAA IQ YRG
Sbjct: 782 QEDESFADMDAEELAEAAMQIQSVFRGIKDKK------------DDSHDLDPVEVIRAALKIQSVYKGVKSRKLNNKPT---PLPQE-----SMDAGDVAEAALRIQYPVYRGFKARKELK-----KENTQIKKDEEQYQLSRAAVTIQTIYRGF 911
BLAST of sperm surface protein sp17 vs. SwissProt
Match: gi|60391785|sp|P62288.1|ASPM_FELCA (RecName: Full=Abnormal spindle-like microcephaly-associated protein homolog) HSP 1 Score: 179.489 bits (454), Expect = 1.180e-43 Identity = 268/1239 (21.63%), Postives = 492/1239 (39.71%), Query Frame = 0
Query: 83 NLSGDDIDLNDPDVAVAATKIQAGYRGSKAR---RDMKS----------RRD-----DESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVA-----------NAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPM--------DIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALR----------QHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELR-QNKEELKDTEAIDIDLEDPE---VERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTF-DIDLEDPE------VERAATKIQAGYKGMRARR---GLRTSAS-IKAEDEIDIDMEDPDEER-AATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNR-KNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQ--------------------AATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSK---MDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDL 1231
+L G N AA ++QA +RG KAR R +++ RRD + K +IK+Q V+ M + + + A + I L +A +A +Q+ YRGM+ R+ + + KIQ+ ++ +RK+ + + +L I Q A Q R +KV + E+ V +++E+ K+QA RG RKQ + + K Q + E+ KAA IQ Y+ + + R L + A T +QA YRG +VR+ +K ++ I AA KIQ ++G RK+ + + + D+ ++ + + A +Q+ Y+G + R Q++R+ QAA +IQ+ ++ + ++ R QH +A + +E ++ AA +Q+ +KG R+++R Q+K + + ++ + +++AA IQ Y+ R + R+ + + I I IQ+ Y+ MR RK ++ E KAA IQ+ YR + ++ A V + +I + + + +++ A KIQA ++G+R RR + T+A+ IKA ++ + R AA IQ YR QG+ ++ A+ LT + +A T +QA ++G+R R+ LR+ Q AA +IQ+ YR + R++ N+ K + +++ + R +++ V + + +A IQAG++GMK R+ LR + AAT IQ +R ++ R+ + L + + + L +++A +Q+ Y+G RK M+ E+ RAAT IQA ++ R + Y +A+ A ++Q +A +QA +R M+ RR +K + V++ +S G + + + + V + + A+ + + + ++A IQ+ +R + A+K +Q + AA+ IQA Y RT A + ++ + + + + + + +A IQA +KGMKAR+ L
Sbjct: 1525 HLKGKTERANYLQKRAAAIQLQAAFRGMKARNLHRQIRAACVFQSYWRMRRDRFRFLNLKKITIKLQAQVR-MHQQLQKYKKIKKAA--LIIQIHLRASVLAKRALASYQKTRSAVIVLQSAYRGMQARRKF------------IHILTSIIKIQSYYRAYISRKKFLRLKHATVKLQSIVKMKQTRKQYLHLRAATLFIQQWYRSIKVAALKREEYV-------QMRESCIKLQAFVRGHLVRKQMRSQRKAAVSLQSYFRMRKMRQHYLEMYKAAVVIQNYYRAYKAQVSQRKNFLQVKR------------AVTCVQAAYRGYKVRQLIK------QQSI-----------AALKIQTAFRGYSKRKKYQYVLQSTIKIQTWYRTYRTVRDVRMQFLKTKAAVISLQSAYRGWKVRTQIRRE-------------------LQAAVRIQSAFRMAQTQKQFRLFKTAALVIQQHLRAWSAGK--KQRMEYTELRNAALMLQSTWKGKIVRRQIRKQHKCAVIIQSYYRMHVQQKKWDIMKKAARLIQMYYRAYRIGRRQRQLYLKTKAAIVI-------------IQSAYRSMRVRKKIK------------------EYNKAAVAIQSTYRAYKAKKNYATYRASAVLIQRWYRNIKIANRQRKEYLNLKKTAVKIQAVFRGIRVRRRIQHMHTAATFIKAMFKMHQAKVRYHKMRTAAVLIQVRYRAY-----CQGK---IQRAK-----YLT---ILKAVTVLQASFRGVRVRQTLRKMQ------------NAAIRIQSCYRRY--------RQQTYFNKLKKVTQTVQQRYRAVKERNVQFQRYN-KLRHSAICIQAGFRGMKARRHLRMM---------------HLAATLIQRRFRTLKMRRRFLSLRKTALWVQRKYRATVCAKHHLQQFL---RLQKAVITLQSSYRGWVVRKKMQ-----------------EMHRAATVIQAAFRMHRAH----VRY--QAVRQASVVIQQRHQANRAAKLQRQRYLRQRHSALILQAAFRSMKARRHLKMMHSSAVLI------QSRFRGLVVRKRFVSLKKAAVFVQRRYRATTCARRHLHQFLKV-------QKAVITIQSSYRRLMAKKKVQA-----------------MHRAAALIQATYKMHRTYVTFQAWKHASILIQQHYRIYRAAKLQRENYVRQRHSALVIQAAYKGMKARQLL 2552 HSP 2 Score: 178.718 bits (452), Expect = 1.892e-43 Identity = 268/1244 (21.54%), Postives = 467/1244 (37.54%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLEL--------DDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRK----------QMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDI----DLDDPEVEKAATKIQAGYKGMRVRREMR---GPALALPSNS-MDADDSSQEL---AATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAI----DIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTF------DIDLEDP-EVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPE-------VDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRRE--VVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMT------------RDGDPFPEEGEPEAIDIDLSDPEV---------ERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERA--------------ATKIQAGYKGMRTRRELKIPYPEEAIHMADPEME---------QAATK-IQAGYRGMRTRRGM------KPIPDVE-----VVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETIS---AATKIQAGFKGMKARRDLSQL 1234
+ K+QA RG R+ M+S+R K ++ +Q + LE+ + A +A++ +V A T +QA YRG K R+ ++ S AA KIQ F+G RK +++ + D+ + + A +Q+ YRG + R Q E+ +AA +IQ+ FR + +KQ + Q+++ + ++ E+ AA +Q+ +KG VRR++R A+ + S M ++ AA IQ YR R+ + + + + I I IQ+ Y+ MR RK+++ E +AA IQ+ Y+ +A+K +A IQ Y+ ++ A RQ +E +N +++ A KIQA ++G+R R+ ++ +A+ + ++ AA IQ Y+ K R K +L A + +QA ++G+R R+++R +++ AA RIQ+ YR R + ++ + ++ + ++ +A IQAG++GM+ARR LR AAT IQ +R ++ RR + + + + +A +Q+ Y+G RKK++E + +AAT IQA +R H+A Y + R+ VV +++ + R +A +QA ++ MK R+ L+ + +A IQ+ +RG+ RK V L + + R F + + I I S + RAA IQA YK RT + + A + + RA A IQA YKGM+ R+ L+ + I + M Q ATK IQ YR + R+ + K V+ +++ ++I + ++ L K +++ + L A V ++ T QA IQ+ +RG + RKGIQ + AA+ IQ+ Y R + D A++ + ++ + E ++ + IQA F+GMK R+ L L
Sbjct: 1732 SCIKLQAFVRGHLVRKQMRSQR----KAAVSLQSYFRMRKMRQHYLEMYKAAVVIQNYYRAYKAQVSQRKNFLQVKRAVTCVQAAYRGYKVRQLIKQQSI------------AALKIQTAFRGYSKRKKYQYVLQSTIKIQTWYRTYRTVRDVRMQFLKTKAAVISLQSAYRGWKVR-------TQIRREL----------QAAVRIQSAFRMAQTQKQFRLFKTAALVIQQHLRAWSAGKKQRMEYTELRNAALMLQSTWKGKIVRRQIRKQHKCAVIIQSYYRMHVQQKKWDIMKKAARLIQMYYRAYRIGRRQRQLYLKTKAAIVI-------------IQSAYRSMRVRKKIK-------------------EYNKAAVAIQSTYRAYKAKKNY-------------------ATYRASAVLIQRWYRNIKI--ANRQRKEYLN-------------LKKTAVKIQAVFRGIRVRRRIQHMHTAATFIKAMFKMHQAKVRYHKMRTAAVLIQVRYRAYCQGKIQRAKYL-------------TILKAVTVLQASFRGVRVRQTLR------------------KMQNAAIRIQSCYRRYRQQTYFNKLKKVTQTVQQRYRAVKERNVQFQRYNKLRHSAICIQAGFRGMKARRHLRMM------------------HLAATLIQRRFRTLKMRRRFLSLRKTALWVQRKYRATVCAKHHLQQFLRLQKAVITLQSSYRGWVVRKKMQE------------MHRAATVIQAAFRMHRAHVRYQAVRQASVVIQQRHQANRAAKLQRQ----------RYLRQRHSALILQAAFRSMKARRHLKMM---------------HSSAVLIQSRFRGLVVRKRFVSLKKAAVFVQRRYRATTCARRHLHQFL-KVQKAVITIQSSYRRLMAKKKVQAMHRAAALIQATYKMHRTYVTF----QAWKHASILIQQHYRIYRAAKLQRENYVRQRHSALVIQAAYKGMKARQLLREKHRAAIIIQSTYRMYRQYLFYRKIQWATKVIQKIYRA-KKRKALQHDALRKVAACVQADFQDMIIRKQIQEQHQAATVLQKHLKASKVRKHYLHFRAKVVFVQRRYRALSAVRT-----QAVICIQSSYRGFKVRKGIQ-----------------RMHLAATLIQSLYRMHRAKLDYRAKKTAVVLIQYYYRSYVRVKTERKNFLALQKSVRIIQAAFRGMKVRQKLKNL 2762 HSP 3 Score: 126.716 bits (317), Expect = 1.250e-27 Identity = 270/1276 (21.16%), Postives = 479/1276 (37.54%), Query Frame = 0
Query: 97 AVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDD-EDEGLELDDPEAEEAELDIDLEDPEV-------ANAATKIQAGYRGMKTRK--------------HLRPASP-IEIDLADPEVEKAATKIQAGFKGMRTRKQMRK---AEPVPEELADIDLDDPE---MNQAATKIQAGYRGMQT-RRGMKVHDKQPEDEVDIDL---------DDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDI----DLDDPEVEKAATKIQAGYKGMRVRR--------------------------EMRGPALALP----SNSMDADDSSQEL----AATKIQAGYRGMRVRKGL---------------------------KATQAENEEGIDIDLDDPEVER------AATKIQAGYKGMRARKELRQHQEEPN----DSEPMDIDLEDPEVERAATKIQAGYKG-MRARKQLKRDNEEEPANEVIDIDLTD---------PEIEQAATKIQAGYKGMRAR---RALRQH----QEEINADEAIDIDLEDPDMER-AATKIQAGYKGMRARKELRQNKEELKDTEA----IDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMA----------ASRIQAGYKGMRTRKSMR--EKAAEAEQGDIDIDL-------NDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDID---LEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMED-------PDEERAATKIQAGYRGMRTRRE-MQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPE----------EFPEESPETGLTEDQ-AATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVE 1195
++AA KIQ +RG R+ + + +IKIQ +T D ++ +A L +V AA +IQ+ +R +T+K HLR S + + E+ AA +Q+ +KG R+Q+RK + + + + + M +AA IQ YR + RR +++ K V I E +AA IQ+ +R KA+K Q +I I + ++K A KIQA ++G+RVRR +MR A+ + + ++ L A T +QA +RG+RVR+ L K TQ + + + + +R +A IQAG++GM+AR+ LR + + + + A +Q Y+ + A+ L++ + A VI + + E+ +AAT IQA ++ RA +A+RQ Q+ A+ A + + +R +A +QA ++ M+AR+ L+ ++ + + +++AA +Q Y+ + + + Q + LMA A+ IQA YK RT + + + A+ Q I N +A IQA Y+GM+ RQ ++ + T+ + L +++ A IQ Y+ + R+ L+ A K + D +D ++ +AAT +Q + + R+ + R ++V + + A IQ+ Y+G + RK ++ + AAT IQ+ YR H+A+ DY +++ V + +S ++ + ++K+ IQA ++GMK R+ L+ L E F ET Q +A +IQ +R +L +++ + +G RAA IQ + K+ E E Q AA +IQ+ + RR + ++AA +Q +R + R+ V+ ++ G LS PQ E L +S + A ++ + KIQA +R +ARK + +V+AA KIQA Y + R++ A ++ ++
Sbjct: 1826 SIAALKIQTAFRGYSKRKKYQY----VLQSTIKIQTWYRTYRTVRDVRMQFLKTKAAVISLQSAYRGWKVRTQIRRELQAAVRIQSAFRMAQTQKQFRLFKTAALVIQQHLRAWSAGKKQRMEYTELRNAALMLQSTWKGKIVRRQIRKQHKCAVIIQSYYRMHVQQKKWDIMKKAARLIQMYYRAYRIGRRQRQLYLKTKAAIVIIQSAYRSMRVRKKIKEYNKAAVAIQSTYRAYKAKKNYATYRASAVLIQRWYRNIKIANRQRKEYLNLKKTAVKIQAVFRGIRVRRRIQHMHTAATFIKAMFKMHQAKVRYHKMRTAAVLIQVRYRAYCQGKIQRAKYLTILKAVTVLQASFRGVRVRQTLRKMQNAAIRIQSCYRRYRQQTYFNKLKKVTQTVQQRYRAVKERNVQFQRYNKLRHSAICIQAGFRGMKARRHLRMMHLAATLIQRRFRTLKMRRRFLSLRKTALWVQRKYRATVCAKHHLQQFLRLQKA--VITLQSSYRGWVVRKKMQEMHRAATVIQAAFRMHRAHVRYQAVRQASVVIQQRHQANRAAKLQRQRYLRQRHSALILQAAFRSMKARRHLKMMHSSAVLIQSRFRGLVVRKRFVSLKKAAVFVQRRYRATTCARRHLHQFLKVQKAVITIQSSYRRLMAKKKVQAMHRAAALIQATYKMHRTYVTFQAWKHASILIQQHYRIYRAAKLQRENYVRQRHSALVIQAAYKGMKARQLLREKHRAAIIIQSTYRMYRQYLFYRKIQWATKVIQKIYRA-KKRKALQHDALRKVAACVQADFQDMIIRKQIQEQHQAATVLQKHLKASKVRKHYLHFRAKVVFVQRRYRALSAVRTQ---AVICIQSSYRGFKVRKGIQR------------MHLAATLIQSLYRMHRAKLDYRAKKTAVVLIQYYYRSYVRVKTERKNFLA--------LQKSVRIIQAAFRGMKVRQKLKNLSEAKMAAIEKRSAFCRHRTETPYEAVQSSALRIQKWHRA----------SLVACSQEAEC-HSQG---------------RAAVTIQKAFC-----KTATEILETQKH-------------AALRIQSFLQMAVYRRRF-------------VQQKRAAVTLQQYFRTWQARKQFLLYRKAASVL------QNHHRGFLS-AKPQR---EAYLHVRSSVIIIQARTRGFIQKRKFQKIKDSTIKIQAAWRSYKARKYL-----------------CKVKAAC-KIQAWYRSWKARKEYLAILKAVKVIQ 2986 HSP 4 Score: 113.62 bits (283), Expect = 1.287e-23 Identity = 184/912 (20.18%), Postives = 353/912 (38.71%), Query Frame = 0
Query: 408 AATKIQAGYKGMRARKELRQHQEEPNDSEPMDID---LEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDL-----------TDPEIEQAATKIQAGYKGMRARRALRQHQEEI----NADEAIDIDLEDPDMERAATKIQAGYKGMRARKE---LRQN----KEELKDTEAIDIDLED-PEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDP-----------EVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTF---DIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLR--ENQALEID-------------LDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGE-------PEAI-------DIDLSDPE------VERAATKIQAGYKGMRTRKSMRE--KAEEQPDAM----DIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADP-EMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDLSQL 1234
AA ++QA ++GM+AR RQ + + +++ K+QA +R +QL++ + + A +I I L + + A +Q+ Y+GM+ARR I + A + ++ A K+Q+ K + RK+ LR ++ + + + E+ ++ + K+QA +G RK MR Q + + L+ E+ AA IQ Y+ + + S R+ N +V++A T +QA YRG + RQ +K + F + V ++ KIQ Y+ R R D+ M+ + A +Q+ YRG + R Q R E+ +AA +IQ+ ++ + +K+ R + AL I ++ +L AA +Q+ ++G + RR++ K + +++ DI ++KAA IQ Y+ + + R L L A IQ+ YR MR RK+I N + + + + A+ +I +++ + +++ A KIQA ++G+R R+ ++ A AM + ++ AA IQ Y+ Y + I A + +A T +QA +RG+R R+ ++ + + + + + R + +K+ + V+ + E + + N + +A IQAGFRGM+AR+ ++ + AA+ IQ + ++ RR + R V+ + + + + A +Q+ ++G R+ + ++
Sbjct: 1541 AAIQLQAAFRGMKARNLHRQIRAACVFQSYWRMRRDRFRFLNLKKITIKLQAQ---VRMHQQLQKYKKIKKAALIIQIHLRASVLAKRALASYQKTRSAVIVLQSAYRGMQARRKFIHILTSIIKIQSYYRAYISRKKFLRLKHATVKLQSIVKMKQTRKQYLHLRAATLFIQQWYRSIKVAALKREEYVQMRESCIKLQAFVRGHLVRKQMRS-----QRKAAVSLQSYFRMRKMRQHYLEMYKAAVVIQNYYRAYKAQVSQRK--------------NFLQVKRAVTCVQAAYRGYKVRQLIKQQSIAALKIQTAFRGYSKRKKYQYVLQSTIKIQTWYRTYRTVR--------------DVRMQFLKTKAAVISLQSAYRGWKVR--TQIRREL------------------QAAVRIQSAFRMAQTQKQFRLFKTAALVIQQHLRAWSAGKKQRMEYTELRNAALMLQSTWKGK------IVRRQIRKQHKCAVIIQSYYRMHVQQKKWDI------MKKAARLIQMYYRAYRIGRRQRQL-----------YLKTKAAIVIIQSAYRSMRVRKKIKEYNKAAVAIQSTYRAYKAKKNYATYRASAVLIQRWYRNIKIANRQRKEYLNLKKTAVKIQAVFRGIRVRRRIQHMHTAATFIKAMFKMHQAKVRYHKMRTAAVLIQVRYRA----------YCQGKIQRAKYLTILKAVTVLQASFRGVRVRQTLRKMQNAAIRI-QSCYRRYRQQTYFNKLKKVTQTVQQRYR---AVKERNVQFQRYN------KLRHSAICIQAGFRGMKARRHLRM-----------------MHLAATLIQRRFRTLKMRRRFLSLRKTALWVQRKYRATVCAKHHLQQFLRLQKAVITLQSSYRGWVVRKKMQEM 2336 HSP 5 Score: 110.153 bits (274), Expect = 1.186e-22 Identity = 191/945 (20.21%), Postives = 355/945 (37.57%), Query Frame = 0
Query: 406 ERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNE----------------EEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDI---DLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVER-----------AATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKS-MREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEE-----RAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEI-----VPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE-----EQPDAMDIDLTDPEV-ERAATKIQAGYKGMRT-RRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRS-ESEGDLSKMDPQNELVEGE--------------LDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQA------------------RKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDL-----AARRPDIETVESNEEVIIGSYPEDDETI---------------------SAATKIQAGFKGMKARRDLSQLRQNRDLMDR 1243
E++A IQ+ Y R KELR++ I L V IQ ++ ++A+K KR E E AN + + AA ++QA ++GM+AR RQ + + ++++ K+QA +R ++L++ K+ K I I L + + A +Q+ Y+GM++R+ +L + +IQ+ Y+ +RK +R K A + I + + K ++A + +Q + ++ + + E ++ + K+QA +G R+ +R+ + M + +AA IQ YR + ++ R + +V RA T +QA Y+G + R+ +++ AA KIQ +RG+ RK Y + V+ + I + + D+ + + A +Q+ Y+G K R +R E QAA +IQ+ +R + +K+ L + + R G+ + ++ E+ AA +Q+ +KG R+ +R++ + + M + ++ ++AA IQ Y+ R RR+ ++ +A A IQ+ YR MR R+ +K V + T R+ +++ + + L++ L+ +A ++ A I + M AAT I+A F+ QA R QG++ +A+ + A + +QA + G+R R+ L AA R ++ + +T+ +A IQAGF+GMKARR L + L+ R
Sbjct: 1466 EKSAVVIQSWY---RMHKELRKY-----------IHLRSCVVI-----IQTRFRCLQAQKSYKRRREAILTIQKFYRAHLKGKTERANYL--------QKRAAAIQLQAAFRGMKARNLHRQIRAACVFQSYWRMRRDRFRFLNLKKITIKLQA---QVRMHQQLQKYKKIKKAALIIQIHLRASVLAKRALASYQKTRSAVIVLQSAYRGMQARRKF-----------------IHILTSIIKIQSYYRAYISRKKFLRLKHATVKLQSI---VKMKQTRKQYLHLRAA--TLFIQQWYRSIKVAALKRE-------EYVQMRESCIKLQAFVRGHLVRKQMRSQRKAAVSLQSYFRMRKMRQHYLEMYKAAVVIQNYYRAYKA--QVSQRKNFL--------------QVKRAVTCVQAAYRGYKVRQLIKQQSI------------AALKIQTAFRGYSKRKKY---QYVLQSTIKIQTWYRTYR-----TVRDVRMQFLKTKAAVISLQSAYRGWKVRTQIR---------------RELQAAVRIQSAFRMAQTQKQFRLFKTAALVIQQHLRAWSA----GKKQRMEY----TELRNAALMLQSTWKGKIVRRQIRKQHKCAVIIQSYYRMHVQQKKWDIMKKAARLIQMYYRAYRIGRRQRQLYLKTKA----------AIVIIQSAYRSMRVRKKIKEYNKAAVAIQS--TYRAYKAKKNYATYRASAVLIQRWYRNIKIANRQRKEYLNLKKTAVKIQAVFRGIRVRRRIQHMHTAATFIKAMFKMHQAKVRYHKMRTAAVLIQVRYRAYCQGKIQRAKYL--------TILKAVTVLQASFRGVRVRQTLRKMQNAAIRIQSCYRRYRQQTYFNKLKKVTQTVQQRYRAVKERNVQFQRYNKLRHSAICIQAGFRGMKARRHLRMMHLAATLIQR 2272
BLAST of sperm surface protein sp17 vs. SwissProt
Match: gi|313104075|sp|P62286.2|ASPM_CANFA (RecName: Full=Abnormal spindle-like microcephaly-associated protein homolog) HSP 1 Score: 169.088 bits (427), Expect = 1.454e-40 Identity = 280/1257 (22.28%), Postives = 471/1257 (37.47%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLEL--------DDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRK----------QMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDI----DLDDPEVEKAATKIQAGYKGMRVRREMR---GPALALPSNS-MDADDSSQEL---AATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAI----DIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ-----GMKPV------EAEPVPEDDTFDIDLEDP-EVERAATKIQAGYKGMRARRGL---------------------------RTSASIKAEDEIDIDMEDPDE-----ERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATK---------------IQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDME--QAATKIQAGFRGMQARKG----------IQGR-----------VAKAEDAF---DIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETIS---AATKIQAGFKGMKARRDLSQL 1234
+ K+QA RG R+ ++ +R +I +Q + LE+ + A +A+++ +V A T +QA YRG K R+ ++ S AA KIQ F+G R RK ++++ + D+ + A +Q +RG + R KQ E ++AA +IQ+ FR KA+KQ K Q+++ + ++ E+ +AA ++Q+ +KG RVRR+++ A+ + SN M ++ AA IQ YR + G K Q + + AA +Q+ Y+ M+ RK+++ E RAA IQ+ Y+ + +K + T+ +A IQ Y+G + + HQ + E ++++ A KIQA Y+G+R R+ R +A+ + ++ AA IQ Y+ R K R K ++ A + +QA ++G R R+++R +++ AAT IQ+ YR R RQ +K V V E +T L+ ++ + IQAG++GM+ARR L +T+ I+ + + + + ++A KIQ+ YRG R++MQ EV RAAT IQA ++ R K + + + + A A K +QA +RG +AR + KN+ S +V +++A +Q Y R T+ A L +A IQ+ YR + A+K+ L EM R + + A+ IQ Y+ R K RE + Q R+A IQA YKGM+ R+ L+ E +AA IQ+ YR R + I V+ E+ + + + L L +AA+A + + I + P E QAAT IQ F+ + RK +Q R V + ++ + + AA++IQ+ Y R + D A++ + +++ I E ++ ++ + IQA F+GMK R L L
Sbjct: 1723 SCIKLQAFVRGHLVRKQIRLQRQA----AISLQSYFRMRKKRQYYLEIYKATLVIQNYYRAYKAQVNQRKNFLQVKRAVTCLQAAYRGYKVRQLIKQQSI------------AALKIQTAFRGYRERKKYQYVLQSTIKIQRWYRTCRTVRDVRTQFLKTRAAVISLQCAFRGWKVR-------KQIRRE----------RQAAVRIQSAFRMAKAQKQFKLLKTAALVIQQHLRAWTAGKRQRMEYIELREAALRLQSTWKGKRVRRQVQKQHKCAVIIQSNYRMYVQQKKWKIMKKAARLIQMYYRAYSI--GRKQRQLY-----------LKTKAAAVVLQSAYRSMKVRKKIK-------------------ECNRAAVTIQSTYRAYKTKK-----------------NYTN--CRASAIIIQRWYRGTK----IANHQRK-----------EYLNLKKTAVKIQAIYRGIRVRRHNRHLHMAATVIQAMFKMHQAKMRYHKMRTAAVIIQVRYRAYREGKIQRAKYL-------------TIMKAITVLQASFRGTRVRQTLR------------------KMQSAATLIQSYYR--RHRQQAYFTKLKKVTRTIQQRYRAVKERNT---QLQRYNKLRHSIICIQAGFRGMKARRHLKSMHLAATLIQRRFRTLRMRRRFLHLRKTAIWIQRKYRATVCAKHYFQHFVRVQKAVIKIQSSYRGWMVRKKMQ--------------------EVHRAATAIQAAFRMHRANVKYQALKHASVVIQQQFRASRAAKLQRQCYLQQRHSALILQAAFRGMKAR----------GHLKNMHSSATLIQSTFRSLVVRKRF--ISLKRATVFVQRKY-----RATICA------RHHLHQFLKLKKAVITIQSSYRRLVAKKK-----LQEMHRAAVLIQATYRMHRTYVTFQTW--KHASILIQQHYRTYRAAKLQRENSVPQ-------------RRSALIIQAVYKGMKARQLLR-------------EKHRAAIIIQSTYRMYRQYLFYQKIQWATKVIQEKY-----------RANKKKALQHSALRNAAAACTEADFQDMI---LRKPTQEQHQAATIIQKHFKASKVRKHYLHLRANVIFVQRRYRALTAVRTQAVVCTQSSYRSCKVQEDMQHRHLAATRIQSFYRMHRAKLDYQAKKTAVAVIQNYFRSYIRVKVEREKFLAVQKSVRIIQAAFRGMKVRERLKSL 2754 HSP 2 Score: 142.895 bits (359), Expect = 1.299e-32 Identity = 273/1291 (21.15%), Postives = 479/1291 (37.10%), Query Frame = 0
Query: 97 AVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDD-EDEGLELDDPEAEEAELDIDLEDPEVAN-------AATKIQAGYRGMKTRK--------------HLRPASPIEID-LADPEVEKAATKIQAGFKGMRTRKQMR---KAEPVPEELADIDLDDPE---MNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVER--------AATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMR-------ARRALRQHQEEINADEAIDIDLEDPD-MERAATKIQAGYKGMRARKELRQNKEELKDT------EAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVER--------AATKIQAGYKGMRAR---RGLRTSASI--KAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLR-------ALPEEFPEESPETGLTEDQAATK-IQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDP--EVERAATKIQAGYKGMRTRKSM------------REKAEEQPDAMDIDLTDPEV------------ERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPE------------------MEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEG-----ELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDL-----NDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGS------YPEDDETIS--------------------AATKIQAGFKGMKARRDLSQLR 1235
++AA KIQ +RG + R+ + + +IKIQ +T D + A L +V AA +IQ+ +R K +K HLR + + + E+ +AA ++Q+ +KG R R+Q++ K + + + + + M +AA IQ YR R KQ + + + + AA +Q+ +R MK RK+ K + Q + + +A IQ Y+G ++ R L L A KIQA YRG+RVR+ + + AAT IQA +K +A+ + + + + +++R A T +QA ++G R R+ L++ ++ AAT IQ+ Y+ R ++ R Q+ A + + L+ + + + IQAG++GM+AR+ L+ L T + + + + A IQ Y+ A+H + + ++ A +IQ+ Y+G RK M+ EV +AAT IQA +R R + ++ V F +++R +A +QA ++GM+AR + + +SA++ + + +RA +Q YR + R+ + + ++ +A IQ+ Y+ + +KKL+E + +AA IQA YR H + YV+ K+ + + R + + S P ++A IQA YKGMK R+ LR + + + Q ATK IQ YR + + L S + R+ E + + D+ L P E +AAT IQ +K + RK R +A + T AAT+IQ+ Y+ R K+ Y + +A + ++++ IQA +RGM+ R +K + +V +V + +S + + Q V G + H AS E P E A Q F M RK + A + + + + + AA +Q + +TRR R ++++ + + Y + +I + KIQA ++ KAR+ L Q++
Sbjct: 1817 SIAALKIQTAFRGYRERKKYQY----VLQSTIKIQRWYRTCRTVRDVRTQFLKTRAAVISLQCAFRGWKVRKQIRRERQAAVRIQSAFRMAKAQKQFKLLKTAALVIQQHLRAWTAGKRQRMEYIELREAALRLQSTWKGKRVRRQVQKQHKCAVIIQSNYRMYVQQKKWKIMKKAARLIQMYYRAYSIGR------KQRQLYL-------KTKAAAVVLQSAYRSMKVRKKIKECNRAAVTIQSTYRAYKTKKNYTNCRASAIIIQRWYRGTKIANHQRKEYLNLKKT------------AVKIQAIYRGIRVRR-----------------HNRHLHMAATVIQAMFKMHQAKMRYHKMRTAAVIIQVRYRAYREGKIQRAKYLTIMKAITVLQASFRGTRVRQTLRK-------------------MQSAATLIQSYYRRHRQQAYFTKLKKVTRTIQQRYRAVKERNTQLQRYNKLRHSIICIQAGFRGMKARRHLKS--MHLAATLIQRRFRTLRMRRRFLHLRKTAIWIQRKYRA--------TVCAKHYFQHFVRVQK-----AVIKIQSSYRGWMVRKKMQ------------------EVHRAATAIQAAFRMHRANVKYQALKHASVVIQQQFRAS-RAAKLQRQCYLQQRHSALILQAAFRGMKARGHLKNMHSSATLIQSTFRSLVVRKRFISLKRATVFVQRKYRA-----TICARHHLHQFL-----------KLKKAVITIQSSYRRLVAKKKLQE------------MHRAAVLIQATYRMH---RTYVT----FQTWKHASILIQQHYRTYRAAKLQRENSVPQ-RRSALIIQAVYKGMKARQLLREKHRAAIIIQSTYRMYRQYLFYQKIQWATKVIQEKYRANKKK----ALQHSAL-RNAAAACTEADFQ--DMILRKPTQEQHQAATIIQKHFKASKVRKHYLHLRANVIFVQRRYRALTAVRTQAVVCTQSSYRSCKVQEDMQHRHLAATRIQSFYRMHRA----KLDYQAKKTAVAVIQNYFRSYIRVKVEREKFLAVQKSVRIIQAAFRGMKVRERLKSLSEVNMVASAK-----QSAFYSCRTEAQCAAVHGSALRTQKWHGASLVTCSQEAE-------YPQRE-ALVATQRAFCEMVTRKLETQKCAALRIQYFLQMAVCRRRFVQQKRAAVTLQQYFRTWQTRRQFLLYRKAAVVLQNHHRAFLSTKHQRQVYLQIRSSIIMIQARTRGFIQKRRFQKIKDSTIKIQAVWRSYKARKYLHQVK 2948 HSP 3 Score: 114.775 bits (286), Expect = 4.893e-24 Identity = 230/1194 (19.26%), Postives = 433/1194 (36.26%), Query Frame = 0
Query: 172 IQAGYRGMKTRK---HLRPASPIEIDLADPEV------EKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDID-------------LDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQA---GYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKR-DNEEEPANEVIDIDLTDP---EIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELR---------QNKEELKDTEAIDIDLEDP----------------------EVERAATKIQAGYKGMRSRKSMREK---AAEHQDEIDIDLEDPE---VLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDID--LNDPEVEK-------AATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDI-------DLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDR-----------AATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRR--EVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAG---YRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRREL----KIPYPEEAIHMADPE----------MEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDLSQLRQNRDLMDRDEDSQTV 1250
IQ+ +R K RK ++ ++ + V EK+A IQ+ + R K++RK ++ IQ +R +Q K++ ++ E + I + + + AA ++Q FR MKAR + +++ Q ++K TK+QA ++ ++ R+++ AL IQ +R K + A+ + + + +Q+ Y+GM+ARK+ + + KIQ+ Y+ +RK+ R N ++ + T A+ IQ Y+ + AL++HQ M + K+QA +G RK++R Q+ ++ +++ +V+RA T +QA Y+G + R+ ++++ A + Q E + VL + +IQ Y+ RT + +R + + I + +V K AA RIQ+ +R + ++ K ++ + +E E+ AA ++Q+ +KG R RR ++ + + A IQ+ YR Q + +I++ A I + + R AA +Q+ Y+ M+ RKK++E +AA IQ+ YR ++ +K+Y + R ++ R + R +++K A KIQA Y+G++ R+ R L T QA K+ Y MR I+ + EG+ + + +A T +QA ++G R R+++R +++ AAT IQ+ Y+ R + K+ + + A E + + IQAG+RGM+ RR +K + ++ + + + + + A+ + + + ++A KIQ+ +RG RK +Q EV AA+ IQA + R A + ++ + + + +A +QA F+GMKAR L + + L+ S V
Sbjct: 1419 IQSMFRRWKRRKMQLQIKATRVLQRAFREWHVRKRAKEEKSAIVIQSWY---RMHKELRKY--------------VQIRSCVVIIQTRFRCLQ---AQKLYKRKKEAILTIQKYYKAYLKGKMERTNYLQKRAAAIRLQTAFRRMKARNLYR-QSRAACVLQSYWRMRQDRFRFLNLKKITTKLQAQVRKHQQLQKYRKIKKAALV-------------------IQIHFRAYVSAKKVLASYQKTRSAVLV-------------LQSAYRGMQARKKF-------------------IHILTSIIKIQSCYRAYISRKRFLRLKNATVKLQSIVKMRQTRKRYLHWRAASLFIQRWYRSAKL-AALKRHQY--------------VQMRESCIKLQAFVRGHLVRKQIRLQRQAAISLQSYFRMRKKRQYYLEIYKATLVIQNYYRAYKAQVNQRKNFLQVKRAVTCLQAAYRGYKVRQLIKQQSIAALKIQTAFRGYRERKKYQYVLQSTIKIQRWYRTCRTVRDVRTQFLKTRAAVISLQCAFRGWKVRKQIRRERQAAVRIQSAFRMAKAQKQFKLLKTAALVIQQHLRAWTAGKRQRMEYIELREAALRLQSTWKGKRVRRQVQ------------------KQHKCAVIIQSNYRMY----VQQKKWKIMKKAARLIQMYYRAYSIGRKQRQLYLKTKAAAVVLQSAYRSMKVRKKIKECN------------RAAVTIQSTYRAYKTKKNYTNCRASAIIIQRWYRGTKIANHQRK----------EYLNLKKTAVKIQAIYRGIRVRRHNRHL---------HMAATVIQAMFKMHQAKMRYHKMRTAAVIIQVRYRAY--------REGKIQRAKY----LTIMKAITVLQASFRGTRVRQTLR-----------------KMQSAATLIQSYYRRHRQQAYFTKLKKVTRTIQQRYRAVKERNTQLQRYNKLRHSIICIQAGFRGMKARRHLKSMHLAATLIQRR---FRTLRMRRRFLHLRKTAIWIQRKYRATVCAKHYFQHFVRV-------QKAVIKIQSSYRGWMVRKKMQ-----------------EVHRAATAIQAAFRMHRANVKYQALKHASVVIQQQFRASRAAKLQRQCYLQQRHSALILQAAFRGMKARGHLKNMHSSATLIQSTFRSLVV 2416
BLAST of sperm surface protein sp17 vs. SwissProt
Match: gi|60391780|sp|P62283.1|ASPM_AOTVO (RecName: Full=Abnormal spindle-like microcephaly-associated protein homolog) HSP 1 Score: 168.318 bits (425), Expect = 2.516e-40 Identity = 267/1265 (21.11%), Postives = 460/1265 (36.36%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLEL--------DDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRK----------QMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDI----DLDDPEVEKAATKIQAGYKGMRVRREM-RGPALALPSNSMDADDSSQ------ELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVI------DIDLTDPE------IEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTF------DIDLEDP-EVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPE------VDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRRE--VVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEE-------------------------FPEESPETGLTED----------QAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAAS---------------AAEVDAYGEPINI-----DMTAPDME-------------QAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETIS---AATKIQAGFKGMKARRDLSQLRQ 1236
+ K+QA RG R+ M+ +R K I +Q + L++ + + +A+++ +V AAT +QA YRG K R+ ++ S AA KIQ+ F+G R R ++++ + L+DI + A +Q+ YRG + R KQ E +AA KIQ+ FR KA+KQ + Q+++ + ++ E+ + +Q+ +KG +RR++ R A+ S D Q + A IQ YR + E + L A +Q+ Y+GM+ RK ++ + +AA IQ+ Y+ + +K+ A+ +I I +T+ + +++ A KIQA Y+G+R RR + QH M RAAT I+A +K ++R + + +A IQ Y+ K RE ++L A + +QA ++G+R R+++R +++ AAT IQ+ YR R + ++ + +I + ++ + IQA ++GM+ARR L+T + I AAT IQ +R + RR + + L + AA KIQ+ Y+ RKK+RE + +AAT IQA +R H+ Y + ++ VV ++ + R +A +QA ++GMKTR+ L+++ F + + +AA IQ+ YR + +K+ L EM R I + A+ IQ Y+ R K RE +Q +A IQA Y+GM+ R+ L+ E +AA IQ+ YR R + + ++ E+ + L NEL + E AS + + + + I + AP + QA IQ+ +RG + R+ IQ + AA++IQ+ Y R D ++ + +++ + + E ++ + IQA F+GMK R+ L + Q
Sbjct: 1727 SCIKLQAFVRGYLVRKQMRLQR----KAVISLQSYFRMRKARQYYLKMYKAVIIIQNYYHSYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIKQQSI------------AAVKIQSAFRGYRKRVKYQSVLQSIIKIQRWYRAYKTLSDIRTHFLKTKAAVISLQSAYRGWKVR-------KQIRRE----------HQAAMKIQSAFRMAKAQKQFRLFKTAALVIQQHLRAWTAGRKQRMEYIELRHSVLMLQSMWKGKTLRRQLQRQHKCAVIIQSYDRMHVQQKKWKIMKKAVLLIQKYYRAYSI----------GREQHHLYLKTKA---AVVTLQSAYRGMKVRKRIK-------------------DCNKAAITIQSKYRAYKTKKKY----AACRASAIIIQRWYRGIKITNHQYKEYLNLKKTAIKIQAVYRGIRVRRHI-QH------------------MHRAATFIKAMFKMHQSR-------------------IRYHTMRKATIVIQVRYRAYYQGKMQRE-------------NYLKILKAVNVLQANFRGVRVRRTLR------------------KLQIAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYRAVKERNIQFQRYNKLRHSVIHIQAIFRGMKARRHLKT---------MHI---------AATLIQRRFRTLMMRRRFLSLKKTAIWVQRKYRAHLCTKHHLQFLRLQNAAIKIQSSYRRWMIRKKIRE------------MHRAATFIQATFRMHRVHMRYQALKQASVVIQQQYQANRAAKLQRQ----------HYLRQRHSAVILQAAFRGMKTRRHLKSMHSSAILIQSRFRSLLVRRRFISLKKAAIFIQRKYRATICAKHKLHQFLQLRKAAITIQSSYRRLMVKKK-----LQEMHRAAVLIQATFRMHRTYITFQTW--KHASILIQQHYRTYRASKLQRENYTKQWH-------------SAVIIQAAYRGMKARQLLR-------------EKHKAAIIIQSTYRMYRQCCLYQKLQWATKIIQEKYRANKKKHKALQ----HNELKKAETCLQASFQDMNIKLIQEQDQTSIIIQKHCKAFKIKKHYLHLRAPVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRRDIQN-----------------MHLAATRIQSFYRMHRATVDYQTKKTAVVLIQNYYRLYVRVKTERKSFLAVQKSVRTIQAAFRGMKVRQKLKNVSQ 2759 HSP 2 Score: 165.236 bits (417), Expect = 2.070e-39 Identity = 244/1141 (21.38%), Postives = 443/1141 (38.83%), Query Frame = 0
Query: 167 NAATKIQAGYRGMKTRK---HLRPASPIEID------LADPE---VEKAATKIQAGFKGMRTRKQ----------MRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDD----PEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALR----------QHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKEL-RQNKEE--LKDTEAIDIDLEDPEV-ERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFD-IDLEDPE------VERAATKIQAGYKGMRARRGL----RTSASIKAEDEIDIDMEDPDEERAAT-KIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNR-KNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQ--------------------AATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDD---ETISAATKIQAGFKGMKARRDL 1231
+A +Q+ YRGM+ RK H+ S I+I ++ E ++ A K+Q+ K +TRKQ +++ + A + +M ++ K+QA RG R+ M++ K A +Q+ FR KAR+ K Q S ++ +V+KAAT +QA Y+G +VR+ ++ Q +AA KIQ+ +RG R R + V ++ KIQ Y+ + ++R H + + A +Q+ Y+G + RKQ++R++ QAA KIQ+ ++ +A++ R QH A +E ++ + +Q+ +KG R++L RQ+K ++ + + + + ++ ++A IQ Y+ Q + + + V + Q+ Y+GM+ RK ++ D N KAA IQ+ YR +T++ A + + I + + + +++ A KIQA Y+G+R RR + R + IKA ++ R AT IQ YR + +MQ N + ++ +A +QA ++G+R R+ LR+ Q AAT IQ+ YR + R++ N+ K I K++ + R +++ + + + + IQA ++GMK R+ L+ T AAT IQ +R + R+ + L + + L ++ AA KIQ+ Y+ RK +R E+ RAAT IQA ++ R + +A+ A ++Q +A +QA +RGM+TRR +K + +++ +S + + + + A+ + + + +AA IQ+ +R + +K +Q E+ AA IQA + RT + ++ + S + + + +A IQA ++GMKAR+ L
Sbjct: 1630 SAVIVLQSAYRGMQARKMYIHI-LTSVIKIQSYYRAYVSKKEFLSLKNATVKLQSIVKMKQTRKQYLHLRATVLFIQRCYRSKKLAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRLQRK-----------------AVISLQSYFRMRKARQYYLKMYKAVIIIQNYYHSYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIK----------------QQSIAAVKIQSAFRGYRKR-----------------VKYQSVLQSIIKIQRWYRAYKTLSDIRTH---------------FLKTKAAVISLQSAYRGWKVRKQIRREH-------------------QAAMKIQSAFRMAKAQKQFRLFKTAALVIQQHLRAWTAGR--KQRMEYIELRHSVLMLQSMWKGKTLRRQLQRQHKCAVIIQSYDRMHVQQKKWKIMKKAVLLIQKYYRAY--------SIGREQHHLYLKTKAAVVTL-----QSAYRGMKVRKRIK-------------DCN-----KAAITIQSKYRAYKTKKKYAACRASAIIIQRWYRGIKITNHQYKEYLNLKKTAIKIQAVYRGIRVRRHIQHMHRAATFIKAMFKMHQSRIRYHTMRKATIVIQVRYRAY-YQGKMQRENYL---------------KILKAVNVLQANFRGVRVRRTLRKLQI------------AATLIQSNYRRY--------RQQTYFNKLKKITKTVQQRYRAVKERNIQFQRYN-KLRHSVIHIQAIFRGMKARRHLK---------------TMHIAATLIQRRFRTLMMRRRFLSLKKTAIWVQRKYRAHLCTKH----HLQFLRLQNAAIKIQSSYRRWMIRKKIR-----------------EMHRAATFIQATFRMHR------VHMRYQALKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSAILIQSRFRSLLVRRRFIS---LKKAAIFIQRKYRATICAKHKLHQFL-------QLRKAAITIQSSYRRLMVKKKLQ-----------------EMHRAAVLIQATFRMHRTYITFQTWKHASILIQQHYRTYRASKLQRENYTKQWHSAVIIQAAYRGMKARQLL 2546 HSP 3 Score: 142.895 bits (359), Expect = 1.487e-32 Identity = 261/1226 (21.29%), Postives = 482/1226 (39.31%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDE----------------GLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTR---KQMRKAEPVPEELADIDL-------DDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSI---DIDLDDP-EVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRK---GLKATQAENEEGIDIDLDDPE------VERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQ---LKRDN----EEEPANEVIDIDLTDPEIEQ--AATKIQAGYKGMRARRALR-QHQEEI---NADEAIDIDLEDPDMERAATKIQAGYKG-MRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMRE--KAA--------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVER--AATKI-QAGYKGMRAR-RGLRTSASIKAEDEIDIDMEDPD------EERAATKIQAGYRGMRTRRE-MQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVE---RAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLT-----DPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARK---GIQGRVAKAEDAFDIDLNDPE---VEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
A +Q+ YRG K R+ +K D +K +I IQ + + + G+++ + + +E ++L+ A KIQA YRG++ R+H++ + +AAT I+A FK ++R MRKA V + + ++ +A +QA +RG++ RR ++ ++Q AAT IQ+ +R + + K + Q+ ++ +I ++ + IQA ++GM+ RR ++ + +AAT IQ +R + +R+ LK T + L ++ AA KIQ+ Y+ RK++R E+ RAAT IQA ++ R + LK+ + ++ AN + + Q +A +QA ++GM+ RR L+ H I + ++ + +++AA IQ Y+ + A+ +L Q ++ +AA IQ+ Y+ + +K ++E +AA H+ I ++ IQ Y+ R K RE N + +A IQA YRGM+ RQ ++ + T+ + + ++ ATKI Q Y+ + + + L+ + KAE + +D + +++ + IQ + + ++ + R +V + + A IQ+ Y+G + R+ D ++ AAT+IQ+ YR H+A DY +++ V +N + + + S V+K+ IQA ++GMK R+ L+ + +E + +Q+A YR +A+ E V R +E + + E RAA IQ ++ M TRK +K + + + +RAA +Q ++ +TR++ + +AA +Q YR + + + + + I ++ +G + K Q S ++ A + ++ AA KIQA +R +A K I V + F L + A+A IQ + + L+A+ + ++I + AA IQA F+G K R+ LRQ
Sbjct: 2019 AVVTLQSAYRGMKVRKRIK----DCNKAAITIQSKYRAYKTKKKYAACRASAIIIQRWYRGIKITNHQYKEY---LNLKKT-----AIKIQAVYRGIRVRRHIQ------------HMHRAATFIKAMFKMHQSRIRYHTMRKATIVIQVRYRAYYQGKMQRENYLKILKAVNVLQANFRGVRVRRTLR-----------------KLQIAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYRAVKERNIQFQRYNKLRHSVIHIQAIFRGMKARRHLK----------------TMHIAATLIQRRFRTLMMRRRFLSLKKTAIWVQRKYRAHLCTKHHLQFLRLQNAAIKIQSSYRRWMIRKKIR-------------------EMHRAATFIQATFRMHRVHMRYQALKQASVVIQQQYQANRAAKLQ-RQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSAILIQSRFRSLLVRRRFISLKKAAIFIQRKYRATICAKHKLHQ----------------FLQLRKAAITIQSSYRRLMVKKKLQEMHRAAVLIQATFRMHRTYITFQTWKHASIL----IQQHYRTYRASKLQRE--------------NYTKQWHSAVIIQAAYRGMKARQLLREKHKAAIIIQSTYRMYRQCCLYQKLQWATKIIQEKYRANKKKHKALQHNELKKAETCLQASFQDMNIKLIQEQDQTSIIIQKHCKAFKIKKHYLHLRAPVVSIQRRYRKLTAVRTQ---AVICIQSYYRGFKVRR------------DIQNMHLAATRIQSFYRMHRATVDYQTKKTAVVLIQNYYR--------LYVRVKTERKSFLAVQKSVRTIQAAFRGMKVRQKLKNVSQE------KMAAIVNQSAL---CCYRS-KAQYEAV--------RSEGVIIQEWYKASCLACSQEAEYHSQSRAAVTIQKAFRRMITRKVETQKCAALRIQFFLQMAVYRRRFVQQKRAAVTLQHYFRMWQTRKQFLL-------------YRKAAVVLQNHYRAFLSAKHQRQV--YLRIRSSVIIIQARIKGFIQKRKFQKI--------RNSTIKIQAMWRRYRAKKSLCKVK-AACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFLNLRASAIIIQRKWRAI-----LSAK------IAHEHFLMIQRH-------RAACLIQAHFRGYKGRQVF--LRQ 3048 HSP 4 Score: 98.5969 bits (244), Expect = 3.812e-19 Identity = 222/1124 (19.75%), Postives = 411/1124 (36.57%), Query Frame = 0
Query: 168 AATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKI-QAGFKGMRTRK---QMRKAEPVPEELADIDLDD-PEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEK--------AATKIQAGYKGMR---VRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKG-MRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTE--------AIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRT--SASIKAEDEI-------DIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLD---EFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVII---GSYPEDDETISAATKIQAGFKGMKARRDLSQL--------RQNRDLMDR 1243
AA IQ +R RK D E+ K+++ I QA F+ + RK Q++ + + L + ++A IQ+ YR KQ + V V+ IQ FR QA+ K ++S I ++E+ AA ++QA ++ ++ + R++R + M D R + ++K + QA + + ++++AA IQ ++ + ARK L +Q+ + A +Q+ Y+GM+ARK I + KIQ+ Y+ +++ E ++ A K+Q+ K + RK+ + + + A E ++ + K+QA +G RK MR Q + I L+ + A + Y M + + + + ++ N +V+KAAT +QA YRG + RQ +K AA KIQ+ ++G R R ++ + IK + DI + A +Q+ YRG + R++++ ++ AA KIQ+ ++ + +K+ R + + + A A G + R +Y+ R V +++ K + R + ++ V++ KI + +K RA E L A +Q+ YRGM+ RK I N + +T + + A +A IQ Y+G++ + + +++ ++ A KIQA Y+G+R RR ++ M +AAT I+A ++ ++R + +V+ ++ R+ +G KM +N L + +A +QA FRG++ R+ ++ +++ AA+ IQ+ Y R + + +TV+ + + ++ + IQA F+GMKARR L + R+ R LM R
Sbjct: 1392 AAVTIQRHWRAYLRRKQ---------DQQRYEMLKSSSLIIQAMFRRWKRRKMQLQVKATITLQRAFREWHLRKRAKEEKSAIVIQSWYRM----------HKQLQKYV-------YVRSCVVIIQKRFRCF----QAQKLYKRRKESILTIQKYYRAYMKGKIERTNYLQKRAAAIQLQAAFRRLKAHNLHRKIRAACVIQSYWRMRQDRV-------------RFLNLKKIIIKLQAHVRKHQQLQ-KYKKMKKAAVTIQTHFQAYIFARKVLASYQKTRS----------------AVIVLQSAYRGMQARKMY-------------------IHILTSVIKIQSYYRAYVSKK-------------------EFLSLKNATVKLQSIVKMKQTRKQYLHLRATVLFIQRCYRSKKLAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRL-----QRKAVISLQSYFRMRKA---RQYYLKMYKAVIIIQNYYHSYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIKQQSI--------------------AAVKIQSAFRGYRKRVKYQSVLQSIIKIQRWYRAYKTLSDIRTHFLKTKAAVISLQSAYRGWKVRKQIRREHQ--------------------AAMKIQSAFRMAKAQKQFRLFKTAALVIQQHLRAWTA--------GRKQRMEYIELRHSVLMLQSMWKGKTLRRQLQRQHKCAVIIQSYDRMHVQQKKWKIMKK-AVLLIQKYYRAYS--IGREQHHLYLKTKAAVVTLQSAYRGMKVRKRIKDCNKAAIT-------IQSKYRAYKTKKKYAACRASAIIIQRWYRGIKITN------HQYKEYLNL-------KKTAIKIQAVYRGIRVRRHIQ-------------HMHRAATFIKAMFKMHQSRIRYHTMRKATIVI--QVRYRAYYQG---KMQRENYL----------------------------KILKAVNVLQANFRGVRVRRTLR-----------------KLQIAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYRAVKERNIQFQRYNKLRHSVIHIQAIFRGMKARRHLKTMHIAATLIQRRFRTLMMR 2275
BLAST of sperm surface protein sp17 vs. SwissProt
Match: gi|60391791|sp|P62294.1|ASPM_PONPY (RecName: Full=Abnormal spindle-like microcephaly-associated protein homolog) HSP 1 Score: 162.54 bits (410), Expect = 1.604e-38 Identity = 274/1274 (21.51%), Postives = 465/1274 (36.50%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLEL--------DDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADID--------LDDPEMNQAATK-----IQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDI----DLDDPEVEKAATKIQAGYKGMRVRREM-RGPALALPSNS---MDADDSSQEL---AATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKS---MREKA--------AEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTF------DIDLEDP-EVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPE------VDRAATKIQAGYKGMRERKKLREN-------QAL----EIDLDDPDLAQAATKIQAGYRGHQA----RKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEG-----------------EPEAIDIDLSD---------PEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERA--------------ATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARK----------GIQGRVAKAEDA--------------FDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETIS---AATKIQAGFKGMKARRDLSQL 1234
+ K+QA RG + R+ M+ +R K I +Q + L++ + A +A+++ +V AAT +QA YRG K R+ ++ S AA KIQ+ F+G R K + V + + I L D + TK +Q+ YRG + R KQ E +AA KIQ+ FR KARKQ + Q+N + ++ E+ A +Q+ +KG +RR++ R A+ S M ++ AA IQ YR + G + + + + A +Q+ Y+GM+ RK ++ + +AA IQ+ Y+ + +K+ +A IQ Y+G++ QH+E +N +++ A KIQ+ Y+G+R R+ ++ + RAAT I+A +K +SR S MR+ A A +Q ++ + + +L A +QA ++G+R R+++R +++ AAT IQ+ YR R + ++ + +I + ++ + IQA ++G +ARR L+ + I AAT IQ +R + RR + + L V A KIQ+ Y+ RK++RE QA+ + + L QA+ IQ Y+ ++A R+ Y+ +R +A +QA ++GMKTR+ L+++ +AT IQ+ +R + R+ + L + F + AI I S E++RAA IQA ++ RT + + A + RA A IQA YKGM+ R+ L+ E +AA IQ+ YR R + + ++ E+ + + K NEL + V A + +NI D QAA IQ + + RK IQ R K F + + + AA+ IQ+ Y R + D ++ I +++ + + E ++ + IQ F+GMK R+ L L
Sbjct: 1725 SCIKLQAFVRGYRVRKQMRLQR----KAVISLQSYFRMRKARQYYLKMYKAIIVIQNYYHAYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIKQQSI------------AALKIQSAFRGYNKRI---KYQSVLQSIIKIQRWYRAYKTLHDTRTHFLKTKAAVISLQSTYRGWKVR-------KQIRRE----------HQAALKIQSAFRMAKARKQFRLFKTAALVIQQNFRAWTAGRKQRMEYIELRHAVLMLQSMWKGKTLRRQLQRQHKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAYSI-------------GREQNHLYLKTKAAVVTLQSAYRGMKVRKRIK-------------------DCNKAAVTIQSKYRAYKTKKKY-------------------ATYRASAIIIQRWYRGIKITN--HQHKEYLN-------------LKKTAIKIQSVYRGIRVRRHIQ-------------------HMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRFRAYYQGKMQHE-KYLTILKAVKILQASFRGVRVRRTLR------------------KMQIAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYRAMKERNIQFQRYNKLRHSVIYIQAIFRGKKARRHLKM---------MHI---------AATLIQRRFRTLMMRRRFLSLKKTAIWIQRKYRAHLYTKHHLQFLRVQNAVIKIQSSYRRWMIRKRMREMHRAATFIQAIFRMRRLHMRYQALKQASVVIQQQYQANRAAKLQRQHYLRQRH-----------------------------------SAVILQAAFRGMKTRRHLKSM---------------HSSATLIQSRFRSLLVRRRFISL------KKATIFVQRKYRATICAKHKLHQFLHLRKSAITIQSSYRRLMVKKKLQEMQRAAVLIQATFRMHRTYITF----QTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVVIQAAYKGMKARQLLR-------------EKHKAAIIIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQ----KAFQHNELKKETC--------VQAGFQDMNIKKQIQDQHQAAIIIQKNCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRKDIQNMHRAATLIQSFYRMHRAKVDYQTKKTAIVVIQNYYRLYVRVKTERKSFLAVQKSVRTIQPAFRGMKVRQKLKNL 2755 HSP 2 Score: 160.999 bits (406), Expect = 4.158e-38 Identity = 247/1139 (21.69%), Postives = 446/1139 (39.16%), Query Frame = 0
Query: 167 NAATKIQAGYRGMKTRK---HLRPASPIEID------LADPE---VEKAATKIQAGFKGMRTRKQ---MRKAEPVPEEL-------ADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDD----PEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQH-------QEEINADEA-IDIDLEDPDMERAATKIQAGYKGMRARKEL-RQNKEELKDTEAIDIDLEDPE---VERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFD-IDLEDPE------VERAATKIQAGYKGMRARRGL----RTSASIKAEDEIDID-MEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNR-KNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQ--------------------AATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDL 1231
+A +Q+ YRGM+ RK H+ S I+I ++ E ++ A K+Q+ K +TRKQ +R A ++ A + +M ++ K+QA RG + R+ M++ K A +Q+ FR KAR+ K Q + ++ +V+KAAT +QA Y+G +VR+ ++ Q +AA KIQ+ +RG R + V ++ KIQ Y+ + + R H + + A +Q+ Y+G + RKQ++R++ QAA KIQ+ ++ +AR+ R Q+ A A +E ++ A +Q+ +KG R++L RQ+K + + ++ + +++AA IQ Y+ Q+ + + + V + Q+ Y+GM+ RK ++ D N KAA IQ+ YR +T++ A + + I + + + +++ A KIQ+ Y+G+R RR + R + IKA ++ + +AA IQ +R QG+ + E + I +A +QA ++G+R R+ LR+ Q AAT IQ+ YR + R++ N+ K I K++ + R +++ + + + + IQA ++G K R+ L+ + AAT IQ +R + R+ + L + + L V+ A KIQ+ Y+ RK MR E+ RAAT IQA ++ R L + Y +A+ A ++Q +A +QA +RGM+TRR +K + ++ +S + V+ + + A+ + ++ + ++A IQ+ +R + +K +Q E++ AA IQA + RT + ++ + + + + I +A IQA +KGMKAR+ L
Sbjct: 1628 SAVIVLQSAYRGMQARKMYIHI-LTSVIKIQSYYRAYVSKKEFLSLKNATIKLQSIVKVKQTRKQYLHLRAAALFIQQCYRSKKIAAQKREEYMQMRESCIKLQAFVRGYRVRKQMRLQRK-----------------AVISLQSYFRMRKARQYYLKMYKAIIVIQNYYHAYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIK----------------QQSIAALKIQSAFRGYNKR-----------------IKYQSVLQSIIKIQRWYRAYKTLHDTRTH---------------FLKTKAAVISLQSTYRGWKVRKQIRREH-------------------QAALKIQSAFRMAKARKQFRLFKTAALVIQQNFRAWTAGRKQRMEYIELRHAVLMLQSMWKGKTLRRQLQRQHKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAY--------SIGREQNHLYLKTKAAVVTL-----QSAYRGMKVRKRIK-------------DCN-----KAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEYLNLKKTAIKIQSVYRGIRVRRHIQHMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRFRAY-----YQGKMQ----HEKYLTI-------LKAVKILQASFRGVRVRRTLRKMQI------------AATLIQSNYRRY--------RQQTYFNKLKKITKTVQQRYRAMKERNIQFQRYN-KLRHSVIYIQAIFRGKKARRHLKMM---------------HIAATLIQRRFRTLMMRRRFLSLKKTAIWIQRKYRAHLYTKH----HLQFLRVQNAVIKIQSSYRRWMIRKRMR-----------------EMHRAATFIQAIFRM----RRLHMRY--QALKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFIS-LKKATIFVQRK--YRATICAKHKLHQFLH-------LRKSAITIQSSYRRLMVKKKLQ-----------------EMQRAAVLIQATFRMHRTYITFQTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVVIQAAYKGMKARQLL 2544 HSP 3 Score: 135.576 bits (340), Expect = 2.572e-30 Identity = 254/1226 (20.72%), Postives = 471/1226 (38.42%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDE----------------GLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTR---KQMRKAEPVPEELADIDLDDPEMN-------QAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSI---DIDLDDP-EVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRK---GLKATQAENEEGIDIDLDDPE------VERAATKIQAGYKGMRARKELRQHQEEPNDSEPM----DIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEA----IDIDLEDPDMERAATKIQAGYKG-MRARKELRQNKEELKDTEAID-------IDLEDPEVERAATKIQAGYKGMRSRKSMRE---------------KAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDID------LEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRRE-MQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLT-----DPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARK---GIQGRVAKAEDAFDIDLNDPE---VEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
A +Q+ YRG K R+ +K D +K ++ IQ + + + G+++ + + +E ++L+ A KIQ+ YRG++ R+H++ + +AAT I+A FK ++R MRKA V + + +A +QA +RG++ RR ++ ++Q AAT IQ+ +R + + K + Q+ ++ +I ++ + IQA ++G + RR ++ +AAT IQ +R + +R+ LK T + L V+ A KIQ+ Y+ RK +R+ + + + + +++A+ IQ Y+ RA K ++ + + VI +QA ++GM+ RR L+ ++ + + +++A +Q Y+ + A+ +L Q K I + + E++RAA IQA ++ R+ + + +AA+ Q E I V+ IQA YKGM+ R+ +REK KAA IQ+ YR R + ++ + + + + E+++ T +QAG++ M +I + D+ +AA IQ + + R+ + R +V + + A IQ+ Y+G + RK D ++ +AAT IQ+ YR H+A+ DY +++ + +N + + + S V+K+ IQ ++GMK R+ L+ L EE + +Q+A YR + + E V SE + + G + + + RAA IQ + M TRK +K + + + +RAA +Q ++ +TR++ + +AA +Q YR + + + + + I ++ S+G + K Q E+ + S ++ A ++ AA KIQA +R +A K I V + F L V A+A IQ + + L+A+ + ++I + A IQA ++G K R+ LRQ
Sbjct: 2017 AVVTLQSAYRGMKVRKRIK----DCNKAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEY---LNLKKT-----AIKIQSVYRGIRVRRHIQ------------HMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRFRAYYQGKMQHEKYLTILKAVKILQASFRGVRVRRTLR-----------------KMQIAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYRAMKERNIQFQRYNKLRHSVIYIQAIFRGKKARRHLKM----------------MHIAATLIQRRFRTLMMRRRFLSLKKTAIWIQRKYRAHLYTKHHLQFLRVQNAVIKIQSSYRRWMIRKRMREMHRAATFIQAIFRMRRLHMRYQALKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVI---------------LQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLHQFLHLRKSAITIQSSYRRLMVKKKLQEMQRAAVLIQATFRMHRTYITFQTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVV-----IQAAYKGMKARQLLREK------------------HKAAIIIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKAFQHNELKKE-TCVQAGFQDM------------------NIKKQIQDQHQAAIIIQKNCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQ---AVICIQSYYRGFKVRK------------DIQNMHRAATLIQSFYRMHRAKVDYQTKKTAIVVIQNYYR--------LYVRVKTERKSFLAVQKSVRTIQPAFRGMKVRQKLKNLSEE------KMAAIVNQSAL---CCYRS-KTQYEAVQ---SEGVMIQEWYKASGLACSQEAEYHSQ--SRAAVTIQKAFCRMATRKLETQKCAALRIQFFLQMAVYRRRFVQQKRAAITLQHYFRTWQTRKQFLL-------------YRKAAVVLQNHYRAFLSAKHQRQV--YLQIRSSVIIIQARSKGFIQKRKFQ------EIKN--STIKIQAIWRRYRAKKYLCKVK-AACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFLNVRASAIIIQRKWRAI-----LSAK------IAHEHFLMIKRH-------RATCLIQAHYRGYKGRQVF--LRQ 3046
BLAST of sperm surface protein sp17 vs. SwissProt
Match: gi|60391792|sp|P62295.1|ASPM_SAGLB (RecName: Full=Abnormal spindle-like microcephaly-associated protein homolog) HSP 1 Score: 160.999 bits (406), Expect = 3.235e-38 Identity = 275/1242 (22.14%), Postives = 469/1242 (37.76%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLEL--------DDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGM--RTRKQ--------MRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDI----DLDDPEVEKAATKIQAGYKGMRVRREM-RGPALALPSNSMDADDSSQ------ELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVID-----IDLTDPE------IEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMR-------TRQGMKPVE--AEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPE------VDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMT------------RDGDPFPEEGEPEAIDIDLSD---------PEVERAATKIQAGYKGMRTRKSMREKAEE----QPDAMDIDLTDPEVE------RAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEME---------QAATK-IQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG----EP-INID-----MTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETIS---AATKIQAGFKGMKARRDL 1231
+ K+QA RG R+ M+ +R K I +Q + L++ + + +A+++ +V AAT +QA YRG K R+ ++ S AA KIQ+ F+G R R Q +++ + L+DI + A +Q+ YRG + R KQ E +AA KIQ+ FR KA+KQ + Q+++ + + E+ +Q+ +KG +RR + R A+ S Q + AA IQ YR + E + L+ A +Q+ Y+GM+ RK + + ++AA IQ+ Y RA K K+ + +I I +T+ + +++ KIQA Y+G+R RR + QH M RAAT I+A +K ++R + + +AA IQ Y+ K RE ++L A + +QA ++G+R R+++R ++ I AAT IQ+ YR R ++ K V+ V E + + ++ + IQA ++GM+ARR L+T AAT IQ +R + RR + + L + AA KIQ+ Y+ RK++RE + +AAT IQA +R H+ + Y + + + + + NR + +A +QA ++GMKTR+ L+++ +A +IQ+ +R + R+ + L + + F + + AI I S EV RAA IQA ++ RT + + Q + + E R+A IQA Y+GM+ R+ L+ + I + M Q ATK IQ YR ++ K + E+ E S + ++ K+ + + A ++ + EP ++I +TA QA IQ+ +RG + R+ IQ + AA++IQ+ Y R + D ++ I +++ + + E ++ + IQA F+GMK R+ L
Sbjct: 324 SCIKLQAFVRGYLVRKQMRLQR----KAVISLQSYYRMRKARQCYLKMYKVVIIIQNYYHSYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIKQQSI------------AAVKIQSAFRGYSKRVRYQSVLQSIIKIQRWYRAYKTLSDIRTHFLKTKAAVISLQSAYRGWKVR-------KQIRRE----------HQAAMKIQSAFRMAKAQKQFRLFKTAALVIQQHLRAWSTGRKQHMQYIELRHPVLMLQSMWKGKTLRRRLQRQHKCAVIIQSYYRMHVQQKKWKTMKKAALLIQKYYRAYSI----------GREQHHLYLETKA---AVVTLQSAYRGMKVRKRI-------------------KDCKKAAVTIQSKY---RAYKTKKKYAAYRSSAIIIQRWYRGIKITNHQYKEYHSLKKTVIKIQAVYRGIRIRRHI-QH------------------MHRAATFIKAMFKMHQSR-------------------IRYHTMRKAAIVIQVRYRAYYQGKMQRE-------------NYLKILRAVNVLQANFRGVRLRRTLR---------NLQI---------AATVIQSNYRRYREQTYFNKLKKITKTVQQRYRAVKERNI--LFQRYNKLRHSVIYIQAVFRGMKARRHLKTM------------------HLAATLIQRRFRTLMMRRRFLSLKKTAIWIQRKYRAHLCTKHHLQFLLLQNAAIKIQSSYRRWMIRKRMRE------------MHRAATFIQATFRMHRVHRRY----QALKQASAMIQQQYQANRAAKLQ----RQHYLRQRHSAVILQAAFRGMKTRRHLKSMHS---------------SAVRIQSRFRSLLVRRRFISLKKAAIFIQRKYRATICAKHKLHQFLKLRKA-AITIQSSYRRLMVKKKLQEVHRAAVLIQATFRMHRTYLTFQTWKHASILIQQHYRTYRASKLQRENYTKQWRSAVIIQAAYRGMKARQLLREKHKAAVIIQSTYRMYRQYCLYQNLQWATKIIQEKYRA--NKKKHKALQHNELKKAETCVQASFQDMNIKKLIQEQHQTSIIIQKHCKAFKIKKHYLHLREPVVSIQRRYRKLTAVH-TQAVICIQSYYRGFKVRRDIQN-----------------MHLAATRIQSFYRMHRAKVDYQTKKTAIVVIQNYYRLYVRVKTERRNFLAVQKSVRTIQAAFRGMKVRQKL 1352 HSP 2 Score: 156.762 bits (395), Expect = 6.610e-37 Identity = 260/1148 (22.65%), Postives = 450/1148 (39.20%), Query Frame = 0
Query: 167 NAATKIQAGYRGMKTRK-HLR-PASPIEID------LADPE---VEKAATKIQAGFKGMRTRKQMR--KAEPV-------PEELADIDLDDP-EMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDP----EVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGM--RVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQH-------QEEINA------DEAIDIDLEDPDMERAATKIQAGYKGMRARKEL-RQNKEELKDTEAIDIDLEDPE---VERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFD-IDLEDPE------VERAATKIQAGYKGMRARRGL----RTSASIKAEDEIDID-MEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNR-KNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQ--------------------AATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPD---MEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDD---ETISAATKIQAGFKGMKARRDL 1231
+A +Q+ YRGM+ RK +++ P S I+I ++ E ++ A K+Q+ K +TRKQ +A V ++LA ++ +M ++ K+QA RG R+ M++ K A +Q+ +R KAR+ K Q S ++ +V+KAAT +QA Y+G +VR+ ++ Q +AA KIQ+ +RG RVR V ++ KIQ Y RA K L DI + + A +Q+ Y+G + RKQ++R++ QAA KIQ+ ++ +A++ R Q+ + A I+L P + +Q+ +KG R+ L RQ+K + + ++ + +++AA IQ Y+ + +++ + LE ++ +Q+ Y+GM+ RK + + +KAA IQ+ YR +T++ + + + I + + + +++ KIQA Y+G+R RR + R + IKA ++ + +AA IQ YR QG+ + E+ + I RA +QA ++G+R R+ LR +L AAT IQ+ YR + R + N+ K I K++ + R +++ + + + + IQA ++GMK R+ L+ T AAT IQ +R + R+ + L + + + L ++ AA KIQ+ Y+ RK MR E+ RAAT IQA ++ R R +A+ A ++Q +A +QA +RGM+TRR +K + V I R S + + + L AA + Y I + +AA IQ+ +R + +K +Q EV AA IQA + RT + ++ + S + + + +A IQA ++GMKAR+ L
Sbjct: 227 SAVILLQSAYRGMQARKMYIQIPTSVIKIQPYYRAYVSKKEFLSLKNATIKLQSIVKMKQTRKQYLHLRATAVFIQQHYRSKKLAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRLQRK-----------------AVISLQSYYRMRKARQCYLKMYKVVIIIQNYYHSYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIK----------------QQSIAAVKIQSAFRGYSKRVR-------------------YQSVLQSIIKIQRWY---RAYKTL------------SDIRTHFLKTKAAVISLQSAYRGWKVRKQIRREH-------------------QAAMKIQSAFRMAKAQKQFRLFKTAALVIQQHLRAWSTGRKQHMQYIELRHPVLM-----LQSMWKGKTLRRRLQRQHKCAVIIQSYYRMHVQQKKWKTMKKAALLIQKYYRAY----------SIGREQHHLYLETKAAVVT---LQSAYRGMKVRKRI------------------KDCKKAAVTIQSKYRAYKTKKKYAAYRSSAIIIQRWYRGIKITNHQYKEYHSLKKTVIKIQAVYRGIRIRRHIQHMHRAATFIKAMFKMHQSRIRYHTMRKAAIVIQVRYRAY-----YQGKMQ----RENYLKI-------LRAVNVLQANFRGVRLRRTLR------------NLQIAATVIQSNYRRY--------REQTYFNKLKKITKTVQQRYRAVKERNILFQRYN-KLRHSVIYIQAVFRGMKARRHLK---------------TMHLAATLIQRRFRTLMMRRRFLSLKKTAIWIQRKYRAHLCTKHHLQFLL----LQNAAIKIQSSYRRWMIRKRMR-----------------EMHRAATFIQATFRMHRVHRRY------QALKQASAMIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSAV----RIQSRFRS------LLVRRRFI--SLKKAAIFIQ-RKYRATICAKHKLHQFLKLRKAAITIQSSYRRLMVKKKLQ-----------------EVHRAAVLIQATFRMHRTYLTFQTWKHASILIQQHYRTYRASKLQRENYTKQWRSAVIIQAAYRGMKARQLL 1143 HSP 3 Score: 135.191 bits (339), Expect = 2.402e-30 Identity = 201/901 (22.31%), Postives = 357/901 (39.62%), Query Frame = 0
Query: 75 LEDGTEVENLS----GDDIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTR---KQMRKAEPVPEELADIDL-------DDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSI---DIDLDDP-EVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRK---GLKATQAENEEGIDIDLDDPE------VERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQ---LKRDN----EEEPANEVIDIDLTDPEIEQ--AATKIQAGYKGMRARRALR-QHQEEINAD---EAIDIDLEDPDMERAATKIQAGYKG-MRARKELRQNKEELKDTEAID-------IDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVE--RAATKI-QAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNE---IVEPAEDPIDI-----DLTDPEVD-------------RAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRRE---VVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEE 900
LE V L G + D AA IQ+ YR K ++ + R +I IQ G+++ + + +E + KIQA YRG++ R+H+ + +AAT I+A FK ++R MRKA V + + ++ +A +QA +RG++ RR ++ +Q AAT IQ+ +R + + K + Q+ ++ +I ++ + IQA ++GM+ RR ++ + LAAT IQ +R + +R+ LK T + L ++ AA KIQ+ Y+ RK +R E+ RAAT IQA ++ R ++ LK+ + ++ AN + + Q +A +QA ++GM+ RR L+ H + ++ + +++AA IQ Y+ + A+ +L Q + K I + + EV RAA IQA ++ R+ + + + +L IQ Y+ R K RE N + ++A IQA YRGM+ RQ ++ V T+ + + + + ATKI Q Y+ A + ++ + ++A T +QA ++ M ++ +Q +++ I++ I L +P V +A IQ+ Y+G + R+ D+ + LA AT+IQ+ YR H+A+ DY +++ V+ N + + RN V+K+ IQA ++GMK R+ L+ + +E
Sbjct: 611 LETKAAVVTLQSAYRGMKVRKRIKDCKKAAVTIQSKYRAYKTKKKYAAYRSS----AIIIQ-------RWYRGIKITNHQYKEYH--------SLKKTVIKIQAVYRGIRIRRHI------------QHMHRAATFIKAMFKMHQSRIRYHTMRKAAIVIQVRYRAYYQGKMQRENYLKILRAVNVLQANFRGVRLRRTLR-----------------NLQIAATVIQSNYRRYREQTYFNKLKKITKTVQQRYRAVKERNILFQRYNKLRHSVIYIQAVFRGMKARRHLK----------------TMHLAATLIQRRFRTLMMRRRFLSLKKTAIWIQRKYRAHLCTKHHLQFLLLQNAAIKIQSSYRRWMIRKRMR-------------------EMHRAATFIQATFRMHRVHRRYQALKQASAMIQQQYQANRAAKLQ-RQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSAVRIQSRFRSLLVRRRFISLKKAAIFIQRKYRATICAKHKLHQFLKLRKAAITIQSSYRRLMVKKKLQEVHRAAVLIQATFRMHRTYLTFQ------------TWKHASIL-----IQQHYRTYRASKLQRE--------------NYTKQWRSAVIIQAAYRGMKARQLLREKHKAAVIIQSTYRMYRQYCLYQNLQWATKIIQEKYR---------------ANKKKHKALQHNELKKAETCVQASFQDMNIKKLIQEQHQTSIIIQKHCKAFKIKKHYLHLREPVVSIQRRYRKLTAVHTQAVICIQSYYRGFKVRR----------DIQNMHLA--ATRIQSFYRMHRAKVDYQTKKTAIVVIQNYYRLYVRVKTERRNF-----------LAVQKSVRTIQAAFRGMKVRQKLKNVSQE 1358 HSP 4 Score: 113.235 bits (282), Expect = 1.082e-23 Identity = 174/714 (24.37%), Postives = 271/714 (37.96%), Query Frame = 0
Query: 103 IQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDP-----EAEEAELDI--DLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELR--QH-----QEEPNDSEPMDIDLEDPEVE-RAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQH--------QEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELR-QNKEEL---KDTEAIDI-----DLEDPEVE-------------RAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREK--AAEAEQGDIDIDL-------NDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPED------DTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRR 756
IQA +RG KARR +K+ + IQ +T+ L L A L L+ + NAA KIQ+ YR RK +R E+ +AAT IQA F+ R + + QA+ IQ Y Q R K+ + + + +A +QA FRGMK R+ K Q S+ + ++KAA IQ Y+ + L L AA IQ+ YR + V+K L EV RAA IQA ++ R + +H Q+ + E+ + R+A IQA Y+GM+AR QL R+ + AA IQ+ Y+ R + L Q+ QE+ A++ L+ ++++A T +QA ++ M +K ++ Q++ + K +A I L +P V +A IQ+ Y+G + R+ D+++ +AA+RIQ+ Y+ R + + K A Q + + N V+K+ IQA +RGM+ RQ +K V E + + + V IQ YK AS A + + E + RAA IQ +R M TR+
Sbjct: 839 IQAVFRGMKARRHLKTMHLAATL----IQRRFRTLMMRRRFLSLKKTAIWIQRKYRAHLCTKHHLQFLLLQNAAIKIQSSYRRWMIRKRMR------------EMHRAATFIQATFRMHR-----------------VHRRYQALKQASAMIQQQY---QANRAAKLQRQHYLRQ----------RHSAVILQAAFRGMKTRRHLKSMHSSAVRIQSRFRSLLVRRRFISLKKAAIFIQRKYRATICAKHKLHQFLKLRK------------AAITIQSSYRRLMVKKKL-----------------QEVHRAAVLIQATFRMHRTYLTFQTWKHASILIQQHYRTYRASKLQRENYTKQWRSAVIIQAAYRGMKAR-QLLREKHK------------------AAVIIQSTYRMYR-QYCLYQNLQWATKIIQEKYRANKKKHKALQHNELKKAETCVQASFQDMNIKKLIQEQHQTSIIIQKHCKAFKIKKHYLHLREPVVSIQRRYRKLTAVHTQAVICIQSYYRGFKVRR---------------DIQNMH--LAATRIQSFYRMHRAKVDYQTKKTAIVVIQNYYRLYVRVKTERRNFLAVQKSVRTIQAAFRGMKVRQKLKNVSQEKIAAIVNRSALYCYGSKAQCEAVGSEGVIIQEWYK-----------ASCFACSQ---EAEYRSQSRAAVTIQKAFRRMITRK 1426
BLAST of sperm surface protein sp17 vs. SwissProt
Match: gi|215273935|sp|Q8IZT6.2|ASPM_HUMAN (RecName: Full=Abnormal spindle-like microcephaly-associated protein; AltName: Full=Abnormal spindle protein homolog; Short=Asp homolog) HSP 1 Score: 160.999 bits (406), Expect = 4.923e-38 Identity = 248/1139 (21.77%), Postives = 444/1139 (38.98%), Query Frame = 0
Query: 167 NAATKIQAGYRGMKTRK---HLRPASPIEID------LADPE---VEKAATKIQAGFKGMRTRKQ---MRKAEPVPEEL-------ADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDD----PEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQH-------QEEINADEA-IDIDLEDPDMERAATKIQAGYKGMRARKEL-RQNKEELKDTEAIDIDLEDPE---VERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFD-IDLEDPE------VERAATKIQAGYKGMRARRGL----RTSASIKAEDEIDID-MEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNR-KNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQ--------------------AATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDL 1231
+A +Q+ YRGM+ RK H+ S I+I ++ E ++ A K+Q+ K +TRKQ +R A ++ A + +M ++ K+QA RG R+ M++ K A +Q+ FR KAR+ K Q + ++ +V+KAAT +QA Y+G +VR+ ++ Q +AA KIQ+ +RG R + V ++ KIQ Y+ + + R H + + A +Q+ Y+G + RKQ++R++ QAA KIQ+ ++ +A++ R Q+ A A +E ++ A +Q+ +KG R++L RQ+K + + ++ + +++AA IQ Y+ Q+ + + + V + Q+ Y+GM+ RK ++ D N KAA IQ+ YR +T++ A + + I + + + +++ A KIQ+ Y+G+R RR + R + IKA ++ + +AA IQ +R R QG+ + E + I +A +QA ++G+R R+ LR+ Q AAT IQ+ YR + R++ N+ K I K++ + +++ + + + + IQA ++G K R+ L+ + AAT IQ +R + R+ + L + + L +V+ A KIQ+ Y+ RK MR E+ RAAT IQ+ ++ R L + Y +A+ A ++Q +A +QA +RGM+TRR +K + ++ +S + V+ + A A+ Y + +AA IQ+ +R + +K +Q E++ AA IQA + RT + ++ + + + + I +A IQA +KGMKAR+ L
Sbjct: 1634 SAVIVLQSAYRGMQARKMYIHI-LTSVIKIQSYYRAYVSKKEFLSLKNATIKLQSTVKMKQTRKQYLHLRAAALFIQQCYRSKKIAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRLQRK-----------------AVISLQSYFRMRKARQYYLKMYKAIIVIQNYYHAYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIK----------------QQSIAALKIQSAFRGYNKR-----------------VKYQSVLQSIIKIQRWYRAYKTLHDTRTH---------------FLKTKAAVISLQSAYRGWKVRKQIRREH-------------------QAALKIQSAFRMAKAQKQFRLFKTAALVIQQNFRAWTAGRKQCMEYIELRHAVLVLQSMWKGKTLRRQLQRQHKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAY--------SIGREQNHLYLKTKAAVVTL-----QSAYRGMKVRKRIK-------------DCN-----KAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEYLNLKKTAIKIQSVYRGIRVRRHIQHMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQ-----VRCRAYYQGKMQ----REKYLTI-------LKAVKVLQASFRGVRVRRTLRKMQT------------AATLIQSNYRRY--------RQQTYFNKLKKITKTVQQRYWAMKERNIQFQRYN-KLRHSVIYIQAIFRGKKARRHLKMM---------------HIAATLIQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKH----HLQFLQVQNAVIKIQSSYRRWMIRKRMR-----------------EMHRAATFIQSTFRMHR----LHMRY--QALKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFIS-LKKATIFVQRKYR-ATICAKHKLYQ--------FLHLRKAAITIQSSYRRLMVKKKLQ-----------------EMQRAAVLIQATFRMYRTYITFQTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVVIQAAYKGMKARQLL 2550 HSP 2 Score: 143.28 bits (360), Expect = 1.226e-32 Identity = 279/1341 (20.81%), Postives = 490/1341 (36.54%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLEL--------DDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADID--------LDDPEMNQAATK-----IQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDI----DLDDPEVEKAATKIQAGYKGMRVRRE--------------------------MRGPALALP----SNSMDADDSSQEL----AATKIQAGYRGMRVRK----------------------------------------GLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVE-----RAATKIQ----AGYKGMRARKQ----LKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMR-------ARRALRQHQEEINADEAIDIDLEDPD-MERAATKIQAGYKGMRARKEL-------------------RQNKEELKDTEAIDI------------DLEDPEVERAATKIQAGYKGMRSRKSMRE--KAAEHQDEI----DIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDL---EDPEVERAATKIQAGYKGMRARRG-------LRTSA-SIKAE-DEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNE----IVEPAEDPIDIDLTDPEVDR---AATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDY--------VSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEK-------AATKIQAGYKGMKTRKT---LRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPD--VEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDL-----NDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDLSQLR 1235
+ K+QA RG R+ M+ +R K I +Q + L++ + A +A+++ +V AAT +QA YRG K R+ ++ S AA KIQ+ F+G R K + V + + I L D + TK +Q+ YRG + R KQ E +AA KIQ+ FR KA+KQ + Q+N + ++ E+ A +Q+ +KG +RR+ M+ AL + + S+ + + L A +Q+ YRGM+VRK G+K T +++E +++ ++ A KIQ+ Y+G+R R+ + QH + + +AA IQ A Y+G R++ LK + + + + T +++ AAT IQ+ Y+ R ++ + Q+ A + +I + + + + IQA ++G +AR+ L R+ LK T AI I L+ +V+ A KIQ+ Y+ RK MRE +AA + + + A+ IQ Y+ R K R+ +A +QA +RGM+TR+ +K + + F L +++A +Q Y+ + LR +A +I++ + + + + +RAA IQA +R RT Q I + L R +A IQA YKGM+ R+ LRE +A+ IQ+ YR ++ Y + + + +N+K + + + N ++ V D +++K AA IQ K K RK LRA + QA IQ+ YRG + RK+I + RAAT IQ+ Y+ R + D E ++ A + Y + R + E +A ++++ IQA +RGM+ R+ +K + + + +V + S+ + + +++ + A +A +AA IQ F M RK + A F + + + + AA +Q + +TR+ R ++++ + + + + S+ IQA KG +R +++
Sbjct: 1731 SCIKLQAFVRGYLVRKQMRLQR----KAVISLQSYFRMRKARQYYLKMYKAIIVIQNYYHAYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIKQQSI------------AALKIQSAFRGYNKRV---KYQSVLQSIIKIQRWYRAYKTLHDTRTHFLKTKAAVISLQSAYRGWKVR-------KQIRRE----------HQAALKIQSAFRMAKAQKQFRLFKTAALVIQQNFRAWTAGRKQCMEYIELRHAVLVLQSMWKGKTLRRQLQRQHKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAYSIGREQNHLYLKTKAAVVTLQSAYRGMKVRKRIKDCNKAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEYLNL-------KKTAIKIQSVYRGIRVRRHI-QHMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRCRAYYQGKMQREKYLTILKAVKVLQASFRGVRVRRTLRKMQTAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYWAMKERNIQFQRYNKLRHSVIYIQAIFRGKKARRHLKMMHIAATLIQRRFRTLMMRRRFLSLKKT-AILIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKRMREMHRAATFIQSTFRMHRLHMRYQALKQASVVIQQQYQANRAAKLQRQHYLRQRH--------------SAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLYQFLHLRKAAITIQSSYRRLMVKKKLQEMQRAAVLIQATFRMYRTYITFQTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVVIQAAYKGMKARQLLREKH------------KASIVIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKK--KQKVFQHNELKKETCVQAGFQDMNIKKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRKDI------------------------------QNMHRAATLIQSFYRMHRAK------------------VDYETKKTAIVVIQNYYRLYVRVK-----TERKNFLA---VQKSVRTIQAAFRGMKVRQKLKNVSEEKMAAIVNQSALCCYRSKTQYEAVQSEGVMIQEWYKASGLACSQEA---------EYHSQSRAAVTIQKAFCRMVTRKLETQKCAALRIQFFLQMAVYRRRFVQQKRAAITLQHYFRTWQTRKQFLLYRKAAVVLQNHYRAFLSAKHQRQVYLQIRSSVIIIQARSKGFIQKRKFQEIK 2933 HSP 3 Score: 128.642 bits (322), Expect = 2.820e-28 Identity = 225/1018 (22.10%), Postives = 377/1018 (37.03%), Query Frame = 0
Query: 103 IQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDP-----EAEEAELDI--DLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVER--------AATKIQAGYKGMRARKQLKRDNEEEPANEVIDI------DLTDPEIEQAATK-IQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMER-------AATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMR--EKAAE--------HQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMK--------------------------PVEAEPVPEDDTFDI-------DLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDM-----EDPDEERAATKIQAGYRGMRTRRE----------MQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRE--NQALEIDLDDPDL--------AQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIV-----PLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERA-ATKIQAGYK 1017
IQA +RG KARR +K + IQ +T+ L L A L L+ +V NA KIQ+ YR RK +R E+ +AAT IQ+ F+ R + + + QA+ IQ Y Q R K+ + + + +A +QA FRGMK R+ K Q S+ + ++KA +Q Y+ + L L AA IQ+ YR + V+K L+ E++RAA IQA ++ R + + + +++R +A IQA YKGM+AR+ L+ E+ A+ VI + Q ATK IQ Y+ + ++ + QH E+ + + +D ++++ AA IQ K + RK + + + L + A IQ+ Y+G + RK ++ +AA H+ ++D + + +++ IQ Y+ K+ R+ N V+K+ IQA +RGM+ RQ +K V++E V + + + E RAA IQ + M R+ L T + + M ++RAA +Q +R +TR++ +Q A+ + L ++ + IQA KG +++K +E N ++I +AA KIQA YR +A K+Y++ + V + + E R + +V +A IQ ++ + LP + E + +AA IQA YRG + R+ + L + + R E G+ E I E +++ +QA +G RK E++ + T A +IQ YK
Sbjct: 2246 IQAIFRGKKARRHLKMMHIAATL----IQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKRMR------------EMHRAATFIQSTFRMHR-----------------LHMRYQALKQASVVIQQQY---QANRAAKLQRQHYLRQ----------RHSAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLYQFLHLRK------------AAITIQSSYRRLMVKKKLQ-----------------EMQRAAVLIQATFRMYRTYITFQTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVVIQAAYKGMKARQLLR---EKHKASIVIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKVFQH-NELKKETCVQAGFQDMNIKKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQ-AVICIQSYYRGFKVRKDIQNMHRAATLIQSFYRMHRAKVDYETKKTAIVV----IQNYYRLYVRVKTERK--------------NFLAVQKSVRTIQAAFRGMKVRQKLKNVSEEKMAAIVNQSALCCYRSKTQYEAVQSEGVMIQEWYKASGLACSQEAEYHSQSRAAVTIQKAFCRMVTRK-LETQKCAALRIQFFLQMAVYRRRFVQQKRAAITLQHYFRTWQTRKQFLLYRKAAVVLQNHYRAFLSAKHQRQVYL---QIRSSVIIIQARSKGFIQKRKFQEIKNSTIKIQAMWRRYRAKKYLCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFL------------NVRASAIIIQRKWRAI--------LPAKIAHEHF-LMIKRHRAACLIQAHYRGYKGRQVFLRQKSAALIIQKYIRA----REAGKHERIKY----IEFKKSTVILQALVRGWLVRKRF---LEQRAKIRLLHFTAAAYYHLNAVRIQRAYK 3129
BLAST of sperm surface protein sp17 vs. SwissProt
Match: gi|60391790|sp|P62293.1|ASPM_PANTR (RecName: Full=Abnormal spindle-like microcephaly-associated protein homolog) HSP 1 Score: 159.073 bits (401), Expect = 1.928e-37 Identity = 247/1142 (21.63%), Postives = 448/1142 (39.23%), Query Frame = 0
Query: 164 EVANAATKIQAGYRGMKTRK---HLRPASPIEID------LADPE---VEKAATKIQAGFKGMRTRKQ---MRKAEPVPEEL-------ADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDD----PEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQH-------QEEINADEA-IDIDLEDPDMERAATKIQAGYKGMRARKEL-RQNKEELKDTEAIDIDLEDPE---VERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFD-IDLEDPE------VERAATKIQAGYKGMRARRGL----RTSASIKAEDEIDID-MEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNR-KNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQ--------------------AATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDL 1231
++ +A +Q+ YRGM+ RK H+ S I+I ++ E ++ A K+Q+ K +TRKQ +R A ++ A + +M ++ K+QA RG R+ M++ K A +Q+ FR KA++ K Q + ++ +V+KAAT +QA Y+G +VR+ ++ Q +AA KIQ+ +RG R + V ++ KIQ Y+ + + R H + + A +Q+ Y+G + RKQ++R++ QAA KIQ+ ++ +A++ R Q+ A A +E ++ A +Q+ +KG R++L RQ+K + + ++ + +++AA IQ Y+ Q+ + + + V + Q+ Y+GM+ RK ++ D N KAA IQ+ YR +T++ A + + I + + + +++ A KIQ+ Y+G+R RR + R + IKA ++ + +AA IQ +R R QG+ + E + I +A +QA ++G+R R+ LR+ Q AAT IQ+ YR + R++ N+ K I K++ + R +++ + + + + IQA ++G K R+ L+ + AAT IQ +R + R+ + L + + L +V+ A KIQ+ Y+ RK MR E+ RAAT IQA ++ R L + Y +A+ A ++Q +A +QA +RGM+TRR +K + ++ +S + V+ + + A+ + ++ + +AA IQ+ +R + +K +Q E++ AA IQA + RT + ++ + + + I +A IQA +KGMKAR+ L
Sbjct: 1631 KIRSAVIVLQSAYRGMQARKMYIHI-LTSVIKIQSYYRAYVSKKEFLSLKNATIKLQSIVKMKQTRKQYLHLRAAALFIQQCYRSKKIAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRLRRK-----------------AVISLQSYFRMRKAQQYYLKMYKAIIVIQNYYHAYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIK----------------QQSIAALKIQSAFRGYNKR-----------------VKYQSVLQSIIKIQRWYRAYKTLHDTRTH---------------FLKTKAAVISLQSAYRGWKVRKQIRREH-------------------QAALKIQSAFRMAKAQKQFRLFKTAALVIQQNFRAWTAGRKQRMEYIELRHAVLMLQSMWKGKTLRRQLQRQHKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAY--------SIGREQNHLYLKTKAAVVTL-----QSAYRGMKVRKRIK-------------DCN-----KAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEYLNLKKTAIKIQSVYRGIRVRRHIQHMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQ-----VRCRAYYQGKMQ----REKYLTI-------LKAVKVLQASFRGVRVRRTLRKMQI------------AATLIQSNYRRY--------RQQTYFNKLKKITKTVQQRYRAMKERNIQFQRYN-KLRHSVIYIQAIFRGKKARRHLKMM---------------HIAATLIQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKH----HLQFLQVQNAVIKIQSSYRRWMIRKRMR-----------------EMHRAATFIQATFRMHR----LHMRY--QALKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFIS-LKKATIFVQRK--YRATICAKHKLHQFLH-------LRKAAITIQSSYRRLMVKKKLQ-----------------EMQRAAVLIQATFRMHRTYITFQTWKHASILIQQHYRTYRAAKLRRENYIRQWHSAVVIQAAYKGMKARQLL 2550 HSP 2 Score: 139.043 bits (349), Expect = 1.892e-31 Identity = 261/1226 (21.29%), Postives = 472/1226 (38.50%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDE----------------GLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTR---KQMRKAEPVPEELADIDLDDP-------EMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSI---DIDLDDP-EVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRK---GLKAT------QAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMR--ARKQ-LKRDN----EEEPANEVIDIDLTDPEIEQ--AATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKE-------ELKDTEAIDIDLED-PEVERAATKIQAGYKGMRSRKSMRE--KAA--------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVER--AATKI-QAGYKGMRARRGLRTSASIKAEDEIDIDMEDPD-------EERAATKIQAGYRGMRTRRE-MQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLT-----DPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARK---GIQGRVAKAEDAFDIDLNDPE---VEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
A +Q+ YRG K R+ +K D +K ++ IQ + + + G+++ + + +E ++L+ A KIQ+ YRG++ R+H++ + +AAT I+A FK ++R MRKA V + + +A +QA +RG++ RR ++ ++Q AAT IQ+ +R + + K + Q+ ++ +I ++ + IQA ++G + RR ++ +AAT IQ +R + +R+ LK T + L +V+ A KIQ+ Y+ RK +R E+ RAAT IQA ++ R R Q LK+ + ++ AN + + Q +A +QA ++GM+ RR L+ M +AT IQ+ ++ + R+ K+ + + T L + +AA IQ+ Y+ + +K ++E +AA H+ I ++ IQ Y+ R K RE N +A IQA Y+GM+ RQ ++ + T+ + + ++ ATKI Q Y+ + ++ +K E + +D + + +AA IQ + + R+ + R +V + + A IQ+ Y+G + RK D ++ +AAT IQ+ YR H+A+ DY +++ V +N + ++ + V+K+ IQA ++GMK R+ L+ + EE + +Q+A YR + + E V SE + + G + + + RAA IQ + M TRK +K + + + +RAA +Q ++ +TR++ + +AA +Q YR + + + + + I ++ S+G + K Q E+ + A A + +AA KIQA +R +A K I V + F L V A+A IQ + + L+A+ + ++I + AA IQA ++G K R+ LRQ
Sbjct: 2023 AVVTLQSAYRGMKVRKRIK----DCNKAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEY---LNLKKT-----AIKIQSVYRGIRVRRHIQ------------HMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRCRAYYQGKMQREKYLTILKAVKVLQASFRGVRVRRTLR-----------------KMQIAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYRAMKERNIQFQRYNKLRHSVIYIQAIFRGKKARRHLKM----------------MHIAATLIQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKRMR-------------------EMHRAATFIQATFRMHRLHMRYQALKQASVVIQQQYQANRAAKLQ-RQHYLRQRHSAVILQAAFRGMKTRRHLK-------------------SMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLHQFLHLRKAAITIQSSYRRLMVKKKLQEMQRAAVLIQATFRMHRTYITFQTWKHASIL----IQQHYRTYRAAKLRRE--------------NYIRQWHSAVVIQAAYKGMKARQLLREKHKASIIIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKAFQHNELKKETCVQAGFQDMNIKKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQ---AVICIQSYYRGFKVRK------------DIQNMHRAATLIQSFYRMHRAKVDYETKKTAVVVIQNYYRLYVRVKTERKNFLA--------VQKSVRTIQAAFRGMKVRQKLKNVSEE------KMAAIVNQSAL---CCYRS-KTQYEAVQ---SEGVMIQEWYKASGLACSQEAECHSQ--SRAAVTIQKAFCRMVTRKLETQKCAALRIQFFLQMAVYRRRFVQQKRAAITLQHYFRTWQTRKQFLL-------------YRKAAVVLQNHYRAFLSAKHQRQV--YLQIRSSVIIIQARSKGFIQKRKFQ-EIKNSTIKIQAMWRRYRAKKYLCKV--------KAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFLNVRASAIIIQRKWRAI-----LSAK------IAHEHFLMIKRH-------RAACLIQAHYRGYKGRQVF--LRQ 3052
BLAST of sperm surface protein sp17 vs. SwissProt
Match: gi|60391793|sp|P62296.1|ASPM_SAIBB (RecName: Full=Abnormal spindle-like microcephaly-associated protein homolog) HSP 1 Score: 158.688 bits (400), Expect = 2.132e-37 Identity = 246/1146 (21.47%), Postives = 441/1146 (38.48%), Query Frame = 0
Query: 167 NAATKIQAGYRGMKTRK---HLRPASPIEID------LADPE---VEKAATKIQAGFKGMRTRKQ---MRKAEPVPEEL-------ADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDD----PEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALR----------QHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKEL-RQNKEELKDTEAIDIDLEDPE---VERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTF-DIDLEDPE------VERAATKIQAGYKGMRARRGL----RTSASIKAEDEIDIDMEDPDEERAAT-KIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNR-KNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQ--------------------AATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDD---ETISAATKIQAGFKGMKARRDLSQLRQ 1236
+A +Q+ YRGM+ RK H+ S I+I ++ E ++ A K+Q+ + +TRKQ +R ++ A + +M ++ K+QA RG R+ M++ K A +Q+ FR K+R+ K Q S ++ +V+KAAT +QA Y+G +VR+ ++ Q +AA KIQ+ +RG R + V ++ KIQ Y+ + +R H + + A +Q+ Y+G + RKQ++R++ QAA KIQ+ ++ +A++ R QH A +E ++ + +Q+ +KG R++L RQ+K + + ++ + +++AA IQ Y+ Q + + + V + Q+ Y+GM+ RK ++ D N KAA IQ+ YR +T++ A + + I + + + +++ A KIQA Y+G+R RR + R + IKA ++ R AT IQ YR QG+ + ED + + +A +QA ++G+R R+ LR+ Q AAT IQ+ YR + R++ N+ K I K++ + R +++ + + + + IQA ++GMK R+ L+ T AAT IQ +R + R+ + L + + L ++ AA KIQ+ Y+ RK MR E+ RAAT IQA ++ R + +A+ A ++Q +A +QA +RGM TRR +K + +++ +S + ++ + A N+ + +AA IQ+ +R + +K +Q E+ AA IQA + +T + ++ + S + + + +A IQA ++GMKAR+ L + R+
Sbjct: 1625 SAVIVLQSAYRGMQARKMYIHI-LTSVIKIQSYYRAYVSKKEFLSLKNATIKLQSIVRMKQTRKQYLHLRATALFIQQCYHSKKLAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRLQRK-----------------AVISLQSYFRMRKSRQYYLKMYKAVIIIQNYYHSYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIK----------------QQSVAAVKIQSAFRGYSKR-----------------VKYQSVLQSIIKIQRWYRAYKTLHGIRTH---------------FLKTKAAVISLQSAYRGWKVRKQIRREH-------------------QAAMKIQSAFRMAKAQKQFRLFKTAALVIQQHLRAWTAGR--KQRMEYIELRHSVLMLQSMWKGKALRRQLQRQHKCAVIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAY--------SIGREQHCLYLKTKAAVVTL-----QSAYRGMKVRKRIK-------------DCN-----KAAITIQSKYRAYKTKKKYAAYRASAIIIQRWYRSIKITNHQYKEYLNLKKTAIKIQAVYRGIRVRRHIQCMHRAATFIKAMFKMHQSRIRYHTMRKATIVIQVRYRAY-----YQGKMQ----REDYL-------KFLKAVNVLQANFRGVRVRRTLRKLQI------------AATVIQSNYRRY--------RQQTYFNKLKKITKTVQQRYRAVKERNIQFQRYN-KLRHSVIHIQAIFRGMKVRRHLK---------------TMHIAATLIQRRFRTLMMRRRFLSLKKTAIWIQRKYRAHLCTKH----HLQFLRLQNAAIKIQSSYRRWVVRKKMR-----------------EMHRAATFIQATFRMHR------VHMRYQALKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMETRRRLKSMHSSAILIQSRFRSLLVRRRFIS-LKKAAIFIQRKYRATICAKH--------NLHQFL-QLRKAAVTIQSSYRRLMVKKKLQ-----------------EMHRAAVLIQATFRMHKTYITFQTWKHASILIQQHYRTYRASKLQRENYTKQWHSALIIQAAYRGMKARQLLREKRK 2546 HSP 2 Score: 141.354 bits (355), Expect = 4.296e-32 Identity = 233/1071 (21.76%), Postives = 416/1071 (38.84%), Query Frame = 0
Query: 92 NDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDP--------EAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMR----KAEPVPEELADID------LDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDP----EVEKAATKIQAGYKGMRVRREMR---GPALALPSNSMDADDSSQELAATK-----IQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVE----RAAT-----KIQAGYKGMRAR----KQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMR-------ARRALRQHQEEINADEAIDIDLEDPD-MERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRK---SMREKAAEHQDEIDIDLEDPEVLM------AASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDID----LEDPEVER---AATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRRE----------MQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEK---AATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREK------AEEQPDAMDI-----DLTDPEVE-------------RAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTR 1058
N V AAT +QA YRG K R+ +K +S ++KIQ + + + A + I + A +Q+ YRG K RK +R +AA KIQ+ F+ + +KQ R A + + L ++ E+ + +Q+ ++G RR ++ K A IQ+ +R +K+ K K Q+ + I + + + A +Q+ Y+GM+VR+ ++ A+ + S A + ++ AA + IQ YR +++ T + +E +++ ++ A KIQA Y+G+R R+ ++ + M + + R AT + +A Y+G R K LK N + + + T +++ AAT IQ+ Y+ R ++ + Q+ A + +I + + + + IQA ++GM+ R+ L+ + AAT IQ ++ + R+ S+++ A Q + L L AA +IQ+ Y+ RK MR E+ +AAT IQA +R R + ++ V + + L+ R +A +QA ++GM RR L++ S +A IQ+ +R + RR +Q + A+ + L ++ +AA IQ+ Y+ + +KKL+E + +AA IQA +R H K Y++ K+ + + R L + K +A IQA Y+GMK R+ LR + +AA IQ+ YR R+ + NL T+ + + L E+++A +QA ++ M +K ++E+ ++ +A I L P V +A IQ+ Y+G + RR+++ M AAT+IQ+ YR R +
Sbjct: 1788 NFLQVKKAATCLQAAYRGYKVRQLIK----QQSVAAVKIQSAFRGYSKRVKYQSVLQSIIKIQRWYRAYKTLHGIRTHFLKTKAAVISLQSAYRGWKVRKQIRRE------------HQAAMKIQSAFRMAKAQKQFRLFKTAALVIQQHLRAWTAGRKQRMEYIELRHSVLMLQSMWKGKALRRQLQRQHK-----------------CAVIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAYSIGREQHCLYLKTKAAVVTLQSAYRGMKVRKRIKDCNKAAITIQSK-YRAYKTKKKYAAYRASAIIIQRWYRSIKI------TNHQYKEYLNL-------KKTAIKIQAVYRGIRVRRHIQCMHRAATFIKAM-FKMHQSRIRYHTMRKATIVIQVRYRAYYQGKMQREDYLKFLKAVNVLQANFRGVRVRRTLRKLQIAATVIQSNYRRYRQQTYFNKLKKITKTVQQRYRAVKERNIQFQRYNKLRHSVIHIQAIFRGMKVRRHLK-------------------TMHIAATLIQRRFRTLMMRRRFLSLKKTAIWIQRKYRAHLCTKHHLQFLRLQNAAIKIQSSYRRWVVRKKMR------------------EMHRAATFIQATFRMHRVHMRYQALKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMETRRRLKSMHS------------------SAILIQSRFRSLLVRRRFISLKKAAIFIQRKYRATICAKHNLHQFL---QLRKAAVTIQSSYRRLMVKKKLQE------------MHRAAVLIQATFRMH---KTYIT----FQTWKHASILIQQHYRTYRAS----KLQRENYTKQWHSALIIQAAYRGMKARQLLRE---------------KRKAAIIIQSTYRMY--RQYCLYQNLQWATKIIQEKYRANKKK--HKALQHNELKKAEACVQASFQDMNIKKLIQEQHQTSLTIQKHCNAFKIKKQYLHLRAPVVSIQRRYRKLTAVHTQAVICIQSYYRGFKVRRDIQ-------------NMHLAATRIQSLYRMHRAK 2697 HSP 3 Score: 141.354 bits (355), Expect = 4.332e-32 Identity = 258/1240 (20.81%), Postives = 462/1240 (37.26%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDE--GLELDDPEAEEAELDIDLEDPE------VANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTR---KQMRKAEPVPEELADIDL-------DDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSI---DIDLDDP-EVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRK---GLKATQAENEEGIDIDLDDPE------VERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRAR---KQLKRDN----EEEPANEVIDIDLTDPEIEQ--AATKIQAGYKGMRARRALR-QHQEEI---NADEAIDIDLEDPDMERAATKIQAGYKG-MRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMRE--------KAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDP---EVERAATKIQAGYKGMRAR-RGLRTSASIKAEDEIDIDMEDPD-------EERAATKIQAGYRGMRTRRE-MQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFN--RNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEE----------------------------FPEESPETGL----------TEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDL---------SDPEVERAATKIQAGYKGMRTRKSM---REKAEEQPDAMDIDLTDP-------EVERAATKIQAGYKGMRTRREL-KIPYPEEAIHMADPEME--------QAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARK----------GIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVE 1195
A +Q+ YRG K R+ +K D +K +I IQ + + + + I + + + + A KIQA YRG++ R+H++ + +AAT I+A FK ++R MRKA V + D + +A +QA +RG++ RR ++ ++Q AAT IQ+ +R + + K + Q+ ++ +I ++ + IQA ++GM+VRR ++ + +AAT IQ +R + +R+ LK T + L ++ AA KIQ+ Y+ RK++R E+ RAAT IQA ++ R + LK+ + ++ AN + + Q +A +QA ++GM RR L+ H I + ++ + +++AA IQ Y+ + A+ L Q ++ +AA IQ+ Y+ + +K ++E +A + I + + A+ IQ Y+ R K RE N + +A IQA YRGM+ RQ ++ + T+ + + ++ A IQ Y+ + + + L+ + KAE + +D + + + + IQ + +++ + R +V + + A IQ+ Y+G + R+ D ++ AAT+IQ+ YR H+A+ DY +++ + +N + RN S V+K+ IQA ++ MK R+ L+ L +E +E +T ++ +AA IQ +R M RK E + A+ I + +RAA +Q ++ +TRK R+ A + L+ ++ + IQA KG +R+ KI I +AA KIQA YR R + I ++ + E L+ M +++ + SA + I QAA IQA FRG + R+ IQ + E + E++ + +QA G R+ + +R I +
Sbjct: 2014 AVVTLQSAYRGMKVRKRIK----DCNKAAITIQSKYRAYKTKKKYAAYRASAIIIQRWYRSIKITNHQYKEYLNLKKTAIKIQAVYRGIRVRRHIQC------------MHRAATFIKAMFKMHQSRIRYHTMRKATIVIQVRYRAYYQGKMQREDYLKFLKAVNVLQANFRGVRVRRTLR-----------------KLQIAATVIQSNYRRYRQQTYFNKLKKITKTVQQRYRAVKERNIQFQRYNKLRHSVIHIQAIFRGMKVRRHLK----------------TMHIAATLIQRRFRTLMMRRRFLSLKKTAIWIQRKYRAHLCTKHHLQFLRLQNAAIKIQSSYRRWVVRKKMR-------------------EMHRAATFIQATFRMHRVHMRYQALKQASVVIQQQYQANRAAKLQ-RQHYLRQRHSAVILQAAFRGMETRRRLKSMHSSAILIQSRFRSLLVRRRFISLKKAAIFIQRKYRATICAKHNLHQ----------------FLQLRKAAVTIQSSYRRLMVKKKLQEMHRAAVLIQATFRMHKTYITFQTWK--HASILIQQHYRTYRASKLQRE--------------NYTKQWHSALIIQAAYRGMKARQLLREKRKAAIIIQSTYRMYRQYCLYQNLQWATKIIQEKYRANKKKHKALQHNELKKAEACVQASFQDMNIKKLIQEQHQTSLTIQKHCNAFKIKKQYLHLRAPVVSIQRRYRKLTAVHTQ---AVICIQSYYRGFKVRR------------DIQNMHLAATRIQSLYRMHRAKVDYQTKKTAIVVIQNYYRLYVRVKTERN----------SFLAVQKSVRTIQAAFRSMKVRQKLKNLSQEKMAAIVSPSAVYCYRIEAQSEAVGSEGVIIQEWYKTSCLAHSQEAEYHSQSRAAVTIQKAFRRMITRK-----------------LETQKCAALRIQFFLQMAVYRRRFVQQKRAAVTLQHYFRTWQTRKQFLLYRKAAVVLQNHYRAFLSAKHQRQVYLQIRSSVIIIQARTKGFIQKRKFQKIKNSTIKIQAVWRRYRDKKSLCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQGSFYXKLERTRFLN-MRASAIIIQRKWRAILSAKIAHEHFLMI-------QRHQAACLIQAHFRGYKGRQVFLRQKSAALNIQKYIRAREAGRRERIKYIELKKSTVTLQALVRGWLVRKRILEQRAKIRLLH 3102
BLAST of sperm surface protein sp17 vs. SwissProt
Match: gi|60391788|sp|P62291.1|ASPM_MACFA (RecName: Full=Abnormal spindle-like microcephaly-associated protein homolog) HSP 1 Score: 158.688 bits (400), Expect = 2.225e-37 Identity = 262/1243 (21.08%), Postives = 462/1243 (37.17%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLEL--------DDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADID--------LDDPEMNQAATK-----IQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDI----DLDDPEVEKAATKIQAGYKGMRVRREM-RGPALALPSNS---MDADDSSQEL---AATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKS---MREKA--------AEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTF------DIDLEDP-EVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDP------EVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRRE--VVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLS---DPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDID---------LTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDL-SKMDPQNELVEGE----------LDHAASAAE--VDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARK----------GIQGRVAKAEDA--------------FDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDL 1231
+ K+QA RG R+ M+ +R K I +Q + L++ + A +A+++ V AAT +QA YRG K R+ ++ S AA KIQ+ F+G R K + V + + I L D TK +Q+ YRG + R KQ E +AA KIQ+ FR KA+KQ + Q+N + ++ E+ A +Q+ +KG +RR++ R A+ S M ++ AA IQ Y+ + E + L A +Q+ Y+GM+ RK ++ + +AA IQ+ Y+ + +K+ +A IQ Y+G++ HQE +N +++ A KIQ+ Y+G+R R+ ++ + RAAT I+A +K +SR S MR+ A A +Q ++ + + +L A +QA ++G+ R ++R +++ AAT IQ+ YR + + ++ + +I + ++ + IQA ++G +ARR L+ AAT IQ +R + RR + + L +V A KIQ+ Y+ RKK+RE + +AAT IQA +R H+ Y + ++ VV ++ + R ++A +QA ++G+KTR+ L+++ +AT IQ+ +R + R+ + L + F + I ++ +AA IQ+ Y+ + +K ++ E Q A+ I +T ++A+ IQ Y+ R + K Y + +A IQ Y+GM+ R+ ++ +++ T R + K+ ++++ + L H E V A + +NI + QAA IQ + + RK IQ R K F + + + AA+ IQ+ Y R + D ++ I +++ + + E + + IQA F+GMK R+ L
Sbjct: 1731 SCIKLQAFVRGYLVRKQMRLQR----KAVISLQSYFRMRKARQYYLKMCKAIIVIQNYYHAYKAQVNQRKNFLRVKKAATCLQAAYRGYKVRQLIKQQSI------------AALKIQSAFRGYNKRV---KYQSVLQSIIKIQRWYRAYKTLHDTRTRFLKTKAAVVSLQSAYRGWKVR-------KQIRRE----------HQAALKIQSAFRMAKAQKQFRLFKTAALVIQQNFRAWTAGRKQRMEYIELRHAVLILQSMWKGKTLRRQLQRQXKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYKAYSI----------GREQHHLYLKTKA---AVVTLQSAYRGMKVRKRIK-------------------DCNKAAVTIQSKYRAYKTKKKY-------------------ATYRASAIIIQRWYRGIKITHX--XHQEYLN-------------LKKTAIKIQSVYRGIRVRRHIQ-------------------HMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRFRAYYQGKMHRE-KYLTILKAVKILQASFRGVXVRWTLR------------------KMQIAATLIQSNYRRYKQQTYFNKLKKITKTIQQRYRAVKERNIQFKRYNKLRHSVIYIQAIFRGKKARRHLKMM------------------HVAATLIQRRFRTLMMRRRFLSLKKTAVWIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKKMRE------------MHRAATFIQATFRMHRVHMRYQALKQASVVIQQQYXANRAAKLQRQ----------HYLRQRRSAVILQAAFRGVKTRRHLKSM---------------HSSATLIQSRFRSLLVRRRFISL------KKATIFVQRKYRATICAKHKLHQFLQLRKAAITIQSSYRRLMVKKKLQ---EMQRAAVLIQATFRMHRTCVTFQTWKQASILIQQHYRTYRAAKLQKENYIRQW---------HSAVVIQTAYKGMKARQHLREKHKAAIIIQS--TYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKALQHNELKKETCVQASFQDMNIQKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRRDIQNMHRAATLIQSFYRMHRAKVDYQTKKTAIVVIQNYYRLYVRVKTERKSFLPVQKSVRTIQAAFRGMKVRQKL 2758 HSP 2 Score: 149.828 bits (377), Expect = 1.183e-34 Identity = 245/1138 (21.53%), Postives = 433/1138 (38.05%), Query Frame = 0
Query: 167 NAATKIQAGYRGMKTRK---HLRPASPIEID------LADPE---VEKAATKIQAGFKGMRTRKQ---MRKAEPVPEELA---DIDLDDPE----MNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPE----VEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQH-------QEEINADEA-IDIDLEDPDMERAATKIQAGYKGMRARKEL-RQNKEELKDTEAIDIDLEDPE---VERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFD-IDL------EDPEVERAATKIQAGYKGMRARRGL----RTSASIKAEDEIDID-MEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQ--------------------AATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDL 1231
+A +Q+ YRGM+ RK H+ S I+I ++ E ++ K+Q+ K +TRKQ +R A ++ I E M ++ K+QA RG R+ M++ K A +Q+ FR KAR+ K Q + ++ + V+KAAT +QA Y+G +VR+ ++ Q +AA KIQ+ +RG R + V ++ KIQ Y RA K L D + + A +Q+ Y+G + RKQ++R++ QAA KIQ+ ++ +A++ R Q+ A A +E ++ A +Q+ +KG R++L RQ K + + ++ + +++AA IQ YK Q + + + V + Q+ Y+GM+ RK ++ D N KAA IQ+ YR +T++ A + + I + E +++ A KIQ+ Y+G+R RR + R + IKA ++ + +AA IQ +R QG+ E + I +A +QA ++G+ R LR+ Q AAT IQ+ YR ++ + + + K I K++ + R +++ + + + + IQA ++G K R+ L+ + AAT IQ +R + R+ + L + + L +V+ A KIQ+ Y+ RK MR E+ RAAT IQA ++ R + +A+ A ++Q +A +QA +RG++TRR +K + ++ +S + V+ + + A+ + + + +AA IQ+ +R + +K +Q E++ AA IQA + RT + ++ + + + + I +A IQ +KGMKAR+ L
Sbjct: 1634 SAVIVLQSAYRGMQARKVYIHI-LTSVIKIQSYYRAYVSKKEFLSLKNTTIKLQSIVKMKQTRKQYLHLRAAALFIQQCYRSKKITTQKREEYMQMRESCIKLQAFVRGYLVRKQMRLQRK-----------------AVISLQSYFRMRKARQYYLKMCKAIIVIQNYYHAYKAQVNQRKNFLRVKKAATCLQAAYRGYKVRQLIK----------------QQSIAALKIQSAFRGYNKR-----------------VKYQSVLQSIIKIQRWY---RAYKTLH------------DTRTRFLKTKAAVVSLQSAYRGWKVRKQIRREH-------------------QAALKIQSAFRMAKAQKQFRLFKTAALVIQQNFRAWTAGRKQRMEYIELRHAVLILQSMWKGKTLRRQLQRQXKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYKAY--------SIGREQHHLYLKTKAAVVTL-----QSAYRGMKVRKRIK-------------DCN-----KAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITHXXHQEYLNLKKTAIKIQSVYRGIRVRRHIQHMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRFRAY-----YQGKMH----REKYLTI-------LKAVKILQASFRGVXVRWTLRKMQI------------AATLIQSNYRRYKQQTYF-------NKLKKITKTIQQRYRAVKERNIQFKRYN-KLRHSVIYIQAIFRGKKARRHLKMM---------------HVAATLIQRRFRTLMMRRRFLSLKKTAVWIQRKYRAHLCTKH----HLQFLQVQNAVIKIQSSYRRWMIRKKMR-----------------EMHRAATFIQATFRMHR------VHMRYQALKQASVVIQQQYXANRAAKLQRQHYLRQRRSAVILQAAFRGVKTRRHLKSMHSSATLIQSRFRSLLVRRRFIS-LKKATIFVQRK--YRATICAKHKLHQFL-------QLRKAAITIQSSYRRLMVKKKLQ-----------------EMQRAAVLIQATFRMHRTCVTFQTWKQASILIQQHYRTYRAAKLQKENYIRQWHSAVVIQTAYKGMKARQHL 2550 HSP 3 Score: 142.51 bits (358), Expect = 1.790e-32 Identity = 264/1287 (20.51%), Postives = 480/1287 (37.30%), Query Frame = 0
Query: 92 NDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDP--------EAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMR----KAEPVPEELADID------LDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEV----EKAATKIQAGYKGMRVRREMR--GPALALPSNSMDADDSSQELA-----ATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVE-----RAATKIQ----AGYKGMRARKQ----LKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMR-------ARRALRQHQEEINADEAIDIDLEDPD-MERAATKIQAGYKGMRARKEL-------------------RQNKEELKDTEAIDI------------DLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEK-----------AATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDL---EDPEVERAATKIQAGYKG-MRARRGLRTSASIK--------AEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNE----IVEPAEDPIDIDLTDPEVDR---AATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDY--------VSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEK-------AATKIQAGYKGMKTRKT---LRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGM--RTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPD--VEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHA--ASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDL-----NDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDLSQLR 1235
N V AAT +QA YRG K R+ +K +S ++KIQ + + + + A + D + A +Q+ YRG K RK +R +AA KIQ+ F+ + +KQ R A + + ++ E+ A +Q+ ++G RR ++ K A IQ+ +R +K+ K K Q+ + I + + + A +Q+ Y+GM+VR+ ++ A + A + ++ A A IQ YRG+++ T ++E +++ ++ A KIQ+ Y+G+R R+ + QH + + +AA IQ A Y+G R++ LK + + + + T +++ AAT IQ+ Y+ + ++ + Q+ A + +I + + + + IQA ++G +AR+ L R+ LK T A+ I L+ +V+ A KIQ+ Y+ RK MRE H+ AA+ IQA ++ R MR +A + I K +A +QA +RG++TR+ +K + + F L +++A +Q Y+ + A+ L ++ + + + + + +RAA IQA +R RT Q + I + L R +A IQ YKGM+ R+ LRE +AA IQ+ YR ++ Y + + + +N+K K+L N ++ V D +++K AA IQ K K RK LRA + QA IQ+ YRG + R++I + RAAT IQ+ Y+ R + D + ++ A + Y + R + E K P ++++ IQA +RGM+ R+ +K + + + +V + S+ + + +++ + A + E + + + +AA IQ FR M RK + A F + + + + AA +Q + +TR+ R ++++ + + + + S+ IQA KG +R +++
Sbjct: 1797 NFLRVKKAATCLQAAYRGYKVRQLIK----QQSIAALKIQSAFRGYNKRVKYQSVLQSIIKIQRWYRAYKTLHDTRTRFLKTKAAVVSLQSAYRGWKVRKQIRRE------------HQAALKIQSAFRMAKAQKQFRLFKTAALVIQQNFRAWTAGRKQRMEYIELRHAVLILQSMWKGKTLRRQLQRQXK-----------------CAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYKAYSIGREQHHLYLKTKAAVVTLQSAYRGMKVRKRIKDCNKAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKI------THXXHQEYLNL-------KKTAIKIQSVYRGIRVRRHI-QHMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRFRAYYQGKMHREKYLTILKAVKILQASFRGVXVRWTLRKMQIAATLIQSNYRRYKQQTYFNKLKKITKTIQQRYRAVKERNIQFKRYNKLRHSVIYIQAIFRGKKARRHLKMMHVAATLIQRRFRTLMMRRRFLSLKKT-AVWIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKKMRE---MHR--------------AATFIQATFRMHRV--HMRYQALKQASVVIQQQYXANRAAKLQRQHYLRQRRSAVILQAAFRGVKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLHQFLQLRKAAITIQSSYRRLMVKKKLQEMQRAAVLIQATFRMHRTCVTFQTWKQASILIQQHYRTYRAAKLQKENYIRQWHSAVVIQTAYKGMKARQHLREKH------------KAAIIIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKK-QKALQH-NELKKETCVQASFQDMNIQKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRRDI------------------------------QNMHRAATLIQSFYRMHRAK------------------VDYQTKKTAIVVIQNYYRLYVRVKTERKSFLP----------VQKSVRTIQAAFRGMKVRQKLKIVSEEKMAAIVNQSALCCYRSKTQYEAVQSEGVMIQEWYKASDLACSQEAECHSQS-----------RAAVTIQNAFRRMVTRKLETQKCAALRIQFFLQMAVYRRRFVQQKRAAITLQHYFRTWQTRKQFLLYRKAAVVLQNHYRAFLSAKHQRQVYLQIRSSVIIIQARSKGFIQKRKFQEIK 2933 HSP 4 Score: 102.834 bits (255), Expect = 2.298e-20 Identity = 181/821 (22.05%), Postives = 316/821 (38.49%), Query Frame = 0
Query: 99 AATKIQAGYRGSKARRDMKSRRDDES-----KQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRT---RKQMRKAEPVPEE------LADIDLDDP-EMNQAATKIQAGYRGMQTRRGMKVHDK-----QPEDEVDIDLDDPEVQEAATKI-QAGFRGMKARKQA--KPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATK------IQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEP---NDSEPMDIDLEDPE-----VERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPE-------IEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEV---ERAATKIQAGYKGMRSRKSM---REKA------------AEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVE--------KAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDP---EVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDI-------DLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQA----ATKIQAGYRGHQARKD 835
+A +QA +RG K RR +KS + +S+ ++ ++ ++ A+ + + ++ AA IQ+ YR + +K L+ E+++AA IQA F+ RT + ++A + ++ A + ++ +A IQ Y+GM+ R+ ++ K Q + + + ATKI Q +R K +++A E K E Q + ++I E +AA IQ K ++R+ L L + + ++L A + IQ+ YRG +VR+ D + RAAT IQ+ Y+ RA+ + + + + + + ++ V+++ IQA ++GM+ R++LK +EE+ A V L ++ IQ YK +D A + E RAA IQ ++ M RK Q L+ + + + +RAA +Q ++ ++RK R+ A A+HQ ++ + + ++ IQA KG + K E + I I KAA +IQA YR R + + F LE V +A IQ ++ + SA I E + I RAA IQA YRG + R+ + + I + E ++ +QA +G RK++ E Q +I L A A +IQ Y+ + A K+
Sbjct: 2387 SAVILQAAFRGVKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLH-QFLQLRKAAITIQSSYRRLMVKKKLQ------------EMQRAAVLIQATFRMHRTCVTFQTWKQASILIQQHYRTYRAAKLQKENYIRQWHSAVVIQTAYKGMKARQHLREKHKAAIIIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKALQHNELKKETCVQASFQDMNIQKQIQEQHQAAIIIQKHCKAFKIRKHY----LHLRATVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRR-----------------DIQNMHRAATLIQSFYRMHRAKVDYQTKKTAIVVIQNYYRLYVRVKTERKSFLPVQKSVRTIQAAFRGMKVRQKLKIVSEEKMAAIVNQSALCCYRSKTQYEAVQSEGVMIQEWYKA---------------SDLACSQEAECHSQSRAAVTIQNAFRRMVTRKLETQKCAALRIQFFLQMAVYRRRFVQQKRAAITLQHYFRTWQTRKQFLLYRKAAVVLQNHYRAFLSAKHQRQVYLQIRSSVII-----IQARSKGF----IQKRKFQEIKNSTIKIQAMWRRYRAKKYLCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTWFLNVRASAIIIQRKWRAI-------LSAKIAHEHFLMIK-----RHRAACLIQAHYRGYKERQVFLRQKSAALIIQKYIRAREAGKRERIKYIEFKKSTVILQALVRGWLVRKRILE-QKTKIRLLHFTAAAYYHLNALRIQRAYKLYLAVKN 3136
BLAST of sperm surface protein sp17 vs. SwissProt
Match: gi|60391786|sp|P62289.1|ASPM_GORGO (RecName: Full=Abnormal spindle-like microcephaly-associated protein homolog) HSP 1 Score: 158.303 bits (399), Expect = 3.169e-37 Identity = 244/1139 (21.42%), Postives = 443/1139 (38.89%), Query Frame = 0
Query: 167 NAATKIQAGYRGMKTRK---HLRPASPIEI------DLADPE---VEKAATKIQAGFKGMRTRKQ---MRKAEPVPEEL-------ADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDD----PEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQH-------QEEINADEA-IDIDLEDPDMERAATKIQAGYKGMRARKEL-RQNKEELKDTEAIDIDLEDPE---VERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFD-IDLEDPE------VERAATKIQAGYKGMRARRGL----RTSASIKAEDEIDID-MEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNR-KNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQ--------------------AATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETI---SAATKIQAGFKGMKARRDL 1231
+A +Q+ YRGM+ RK H+ S I+I ++ E ++ A K+Q+ K +TRKQ +R A ++ A + +M ++ K+QA RG R+ M++ K A +Q+ FR KAR+ K Q + ++ +V+KAAT +QA Y+G +VR+ ++ Q +AA KIQ+ +RG R + V ++ KIQ Y+ + + R H + + A +Q+ Y+G + RKQ++R++ QA KIQ+ ++ +A++ R Q+ A A +E ++ A +Q+ ++G R++L RQ+K + + ++ + +++AA IQ Y+ Q+ + + + V + Q+ Y+GM+ RK ++ D N KAA IQ+ YR +T++ A + + I + + + +++ A KIQ+ Y+G+R RR + R + IKA ++ + +AA IQ +R QG+ + E + I +A +QA ++G+R R+ LR+ Q AAT IQ+ YR + R++ N+ K I K++ + R +++ + + + + IQA ++G K R+ L+ + AAT IQ +R + R+ + L + + L +V+ A KIQ+ Y+ RK MR E+ RAAT IQA ++ R L + Y + + A ++Q +A +QA +RGM+TRR +K + ++ +S + V+ + + A+ + ++ + +AA IQ+ +R + +K +Q E++ AA IQA + RT + ++ + + + + I +A IQA +KGMKAR L
Sbjct: 1633 SAVIVLQSAYRGMQARKMYVHI-LTSVIKIQSYYRAHVSKKEFLSLKNATIKLQSIVKMKQTRKQYLHLRAAALFIQQCYRSKKIAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRLQRK-----------------AVISLQSYFRMRKARQYYLKMYKAIIVIQNYYHAYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIK----------------QQSIAALKIQSAFRGYNKR-----------------VKYQSVLQSIIKIQRWYRAYKTLHDTRTH---------------FLKTKAALISLQSAYRGWKVRKQIRREH-------------------QAVLKIQSAFRMAKAQKQFRLFKTAALVIQQNFKAWTAGRKQRMEYIELRHAVLMLQSMWRGKTLRRQLQRQHKCAVIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAY--------SIGREQNHLYLKTKAAVVTL-----QSAYRGMKVRKRIK-------------DCN-----KAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEYLNLKKTAIKIQSVYRGIRVRRHIQHMHRAATFIKAVFKMHQSRISYHTMRKAAIVIQVRFRAY-----YQGKTQ----REKYLTI-------LKAVKILQASFRGVRVRRTLRKMQI------------AATLIQSNYRRY--------RKQTYFNKLKKITKTVQQRYRAMKERNIQFQRYN-KLRHSVIYIQAIFRGKKARRHLKMM---------------HIAATLIQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKH----HLQFLQVQNAVIKIQSSYRRWMIRKRMR-----------------EMHRAATFIQATFRMHR----LHMRY--QTLKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFIS-LKKATIFVQRK--YRATICAKHKLHQFLH-------LRKAAITIQSSYRRLMVKKKLQ-----------------EMQRAAILIQATFRMHRTYTTFQTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVVIQAAYKGMKARHLL 2549 HSP 2 Score: 138.272 bits (347), Expect = 3.773e-31 Identity = 237/1086 (21.82%), Postives = 411/1086 (37.85%), Query Frame = 0
Query: 92 NDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDP--------EAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMR----KAEPVPEELADID------LDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEV----EKAATKIQAGYKGMRVRREMR--GPALALPSNSMDADDSSQELA-----ATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDL-------TDPE----IEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAI----------DIDLEDP-EVERAATKIQAGYKGMRSRKSM--------------------REKAAEHQDEIDI------------DLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDID----LEDPEVER---AATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRREM----------QGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLR-------ALPEEFPEESPETGLTEDQAATK-IQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVER-----------AATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTR 1058
N V AAT +QA YRG K R+ +K +S ++KIQ + + + + A + D + A +Q+ YRG K RK +R +A KIQ+ F+ + +KQ R A + + ++ E+ A +Q+ +RG RR ++ K A IQ+ +R +K+ K K Q+ + I + + + A +Q+ Y+GM+VR+ ++ A + A + ++ A A IQ YRG+++ T +++E +++ ++ A KIQ+ Y+G+R R+ + QH + RAAT I+A +K ++R + A VI + T E I +A +QA ++G+R RR LR+ M+ AAT IQ+ Y+ R RK+ NK + K T+ + +I + ++ + IQA ++G ++R+ + R + + I I L+ +V A +IQ+ Y+ RK MR E+ +AAT IQA +R R + ++ V + + L+ R +A +QA ++GM+ RR L++ S +AT IQ+ +R + RR Q + A+ + L + +AA IQ+ Y+ + +KKL+E Q +AA IQA +R H+ + + + + ++ E+ I +A IQA YKGMK R LR + + + Q ATK IQ YR + +++ N E+ ++ + + ++I E +AA IQ K + RK + + T ++R A IQ+ Y+G + R++++ M QAAT IQ+ YR R +
Sbjct: 1796 NFLQVKKAATCLQAAYRGYKVRQLIK----QQSIAALKIQSAFRGYNKRVKYQSVLQSIIKIQRWYRAYKTLHDTRTHFLKTKAALISLQSAYRGWKVRKQIRRE------------HQAVLKIQSAFRMAKAQKQFRLFKTAALVIQQNFKAWTAGRKQRMEYIELRHAVLMLQSMWRGKTLRRQLQRQHK-----------------CAVIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAYSIGREQNHLYLKTKAAVVTLQSAYRGMKVRKRIKDCNKAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKI------TNHQHKEYLNL-------KKTAIKIQSVYRGIRVRRHI-QH------------------MHRAATFIKAVFKMHQSRISY---HTMRKAAIVIQVRFRAYYQGKTQREKYLTILKAVKILQASFRGVRVRRTLRK-------------------MQIAATLIQSNYR--RYRKQTYFNKLK-KITKTVQQRYRAMKERNIQFQRYNKLRHSVIYIQAIFRGKKARRHLKMMHIAATLIQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKRMR------------------EMHRAATFIQATFRMHRLHMRYQTLKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMKTRRHLKSMHS------------------SATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLHQFL---HLRKAAITIQSSYRRLMVKKKLQEMQ------------RAAILIQATFRMHRTYTTFQTWKHASILIQQHYRTYRAAKLQRENYIRQWH--------SAVVIQAAYKGMKARHLLREKHKASIIIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKAFQHN--ELKKETCV---QAGFQDMNIKKQIQEQHQAAIIIQKHCKAFKIRK----------HYLHLRATVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRKDIQ-------------NMHQAATLIQSFYRMHRAK 2704 HSP 3 Score: 137.887 bits (346), Expect = 4.816e-31 Identity = 257/1200 (21.42%), Postives = 454/1200 (37.83%), Query Frame = 0
Query: 168 AATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMR----KAEPVPEELADIDLDDPE------MNQAATKIQAGYRGMQTRRGMK-VHDKQPEDEVDIDLDDPEV-----QEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMR-------VRKGLKATQ----AENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQ---LKRD----NEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAI----DIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSR---KSMREKAAEHQDEID--------IDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLND---------PEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDTFDIDLEDPEVER--------AATKIQAGYKGMRARRGLR--TSASI----------------KAEDEIDIDME------------DPDEERAATKIQAGYRGMRTRREMQGRNE---IVEPA-------EDPIDIDLTDPEVDR-----------AATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRRE---VVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLT-----DPEVERAATKIQAGYKGMRTRRELKIPYPEEAIHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARK---GIQGRVAKAEDAFDIDLNDPE---VEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
A +Q+ YRGMK RK ++ + KAA IQ+ ++ +T+K+ A + I + + + + + A KIQ+ YRG++ RR ++ +H + + + ++AA IQ FR A + K + + I KA +QA ++G+RVRR +R ++AAT IQ+ YR R ++K K Q A E I + ++ + IQA ++G +AR+ L+ M I AAT IQ ++ + R++ LK+ + A+ L +++ A KIQ+ Y+ R+ +R+ +A + + +++A+ IQ Y+ RA K RQ+ + + I +QA ++GM++R KSM A Q I L+ + +Q Y+ K + + I I + E+++AA IQA +R RT + + + + +++R +A IQA YKGM+AR LR ASI K + I E +E + T +QAG++ M ++++Q +++ I++ + + + T + R A IQ+ Y+G + RK D ++ QAAT IQ+ YR H+A+ DY +++ V+ N + + +N V+K+ IQA ++GM+ R+ L+ + EE + +Q+A YR + + E V SE + + G + + + RAA IQ + M TRK +K + + + +RAA +Q ++ +TR++ + +AA +Q YR + + + + + I ++ S+G + K Q E+ + A A + +AA KIQA +R +A K I V + F L V A+A IQ + + L+A+ + ++I + AA IQA ++G K R+ LRQ
Sbjct: 2022 AVVTLQSAYRGMKVRKRIK------------DCNKAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEYLNLKKTAIKIQSVYRGIRVRRHIQHMHRAATFIKAVFKMHQSRISYHTMRKAAIVIQVRFR-------AYYQGKTQREKYLTIL------------KAVKILQASFRGVRVRRTLR----------------KMQIAATLIQSNYRRYRKQTYFNKLKKITKTVQQRYRAMKERNIQFQRYN-KLRHSVIYIQAIFRGKKARRHLKM----------MHI---------AATLIQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKRMREMHRAATFIQATFRMHRLHMRYQTLKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVI---------------LQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIF-----VQRKYRATICAKHKLHQFLHLRKAAITIQSSYRRLMVKKKLQEMQRAAILIQATFRMHRTYTTFQTWKHASILIQQHYR-TYRAAKLQRENYIRQWHSAVVIQAAYKGMKARHLLREKHKASIIIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKAFQHNELKKETCVQAGFQDMNIKKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRK------------DIQNMHQAATLIQSFYRMHRAKVDYETKKTAIVVIQNYYRLYVRVKTERKNF-----------LAVQKSVRTIQAAFRGMEVRQKLKNVSEE------KVAAIVNQSAL---CCYRS-KTQYEAVQ---SEGVMIQEWYKASGLACSQEAEYHSQ--SRAAVTIQKAFCRMATRKLETQKYAALRIQFFLQMAVYRRRFVQQKRAAITLQHYFRTWQTRKQFLL-------------YRKAAVVLQNHYRAFLSAKHQRQV--YLQIRSSVIIIQARSKGFIQKRKFQ-EIKNSTIKIQAMWRRYRAKKYLCKV--------KAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFLNVRASAIIIQRKWRAI-----LSAK------IAHEHFLMIKRH-------RAACLIQAHYRGYKGRQVF--LRQ 3051 HSP 4 Score: 126.331 bits (316), Expect = 1.767e-27 Identity = 212/981 (21.61%), Postives = 352/981 (35.88%), Query Frame = 0
Query: 103 IQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDP-----EAEEAELDI--DLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVER--------AATKIQAGYKGMRARKQLKRDNEEEPANEVIDID-------LTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMER-------AATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRK---SMREKAA-------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPED------DTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDIDMEDPDEERAATKIQAGYRGMRTRR-EMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSL------DEFNRNIEDDIVDIDLSDP---------DVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTR 1022
IQA +RG KARR +K + IQ +T+ L L A L L+ +V NA KIQ+ YR RK +R E+ +AAT IQA F+ R + + + QA+ IQ Y Q R K+ + + + +A +QA FRGMK R+ K Q S+ + ++KA +Q Y+ + L L AA IQ+ YR + V+K L+ E++RAA IQA ++ R + + + +++R +A IQA YKGM+AR L+ E+ A+ +I +++ A IQ Y+ + ++ QH E+ + + +D ++++ AA IQ K + RK + + + L + A IQ+ Y+G + RK +M + A H+ ++D + + +++ IQ Y+ K+ R+ N V+K+ IQA +RGM RQ +K V E V + + V+ IQ YK GL S + E + RAA IQ + M TR+ E Q AA +IQ + R++ + + +AA +Q +R Q RK ++ R+ +N ++ + I ++ I +++ + KIQA ++ + +K L + +AA KIQA YR RA KE + + + G + + +++ S A IQ ++ + + K E + RAA IQA Y+G + R
Sbjct: 2245 IQAIFRGKKARRHLKMMHIAATL----IQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKRMR------------EMHRAATFIQATFRMHR-----------------LHMRYQTLKQASVVIQQQY---QANRAAKLQRQHYLRQ----------RHSAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLHQFLHLRK------------AAITIQSSYRRLMVKKKLQ-----------------EMQRAAILIQATFRMHRTYTTFQTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVVIQAAYKGMKARHLLR---EKHKASIIIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKAFQH-NELKKETCVQAGFQDMNIKKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQ-AVICIQSYYRGFKVRKDIQNMHQAATLIQSFYRMHRAKVDYETKKTAIVV----IQNYYRLYVRVKTERK--------------NFLAVQKSVRTIQAAFRGMEVRQKLKNVSEEKVAAIVNQSALCCYRSKTQYEAVQSEGVMIQEWYKA----SGLACSQ----------EAEYHSQSRAAVTIQKAFCRMATRKLETQ----------------------KYAALRIQFFLQMAVYRRRFVQQK------------RAAITLQHYFRTWQTRKQFLLYRKAAVVLQNHYRAFLSAKHQRQVYLQIRSSVIIIQARSKGFIQKRKFQEIKNSTIKIQAMWRRYRAKKY----------------LCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFLNVRAS-------AIIIQRKWRAILSAKIAHE-----------HFLMIKRHRAACLIQAHYRGYKGR 3045
BLAST of sperm surface protein sp17 vs. nr
Match: gi|1325261155|ref|XP_023330573.1| (kinesin-related protein 4-like isoform X1 [Eurytemora affinis] >gi|1325261161|ref|XP_023330578.1| kinesin-related protein 4-like isoform X1 [Eurytemora affinis]) HSP 1 Score: 789.645 bits (2038), Expect = 0.000e+0 Identity = 672/2070 (32.46%), Postives = 1023/2070 (49.42%), Query Frame = 0
Query: 88 DIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRD-------DESKQSIKIQMPVQTMDDEDE--------GLELDDPEAEE--AELDIDLEDPEVANAATKIQAGYRGMKTRKHLR-----------------------------PASPIE--IDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMK-VHDKQ-----------------------PEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEE---DSQENI------------PSIDIDLDDPEVEKAATKIQAGYKGMRVRREMR---------------------GPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAE--------------------NEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPN-------------------DSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKR--DNEE--EPANEV--------IDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEE-------------------INA---DEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEE---LKDTE---------------AIDIDLEDPEVERAATKIQAGYKGMRSR---------KSMREKAAEHQD-------EIDIDLEDPEVLMAASRIQAGYKGMRTRK---SMREK---AAEAEQGD-------------IDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPV----------------EAEPVPEDDT--FDIDLEDPEVERAATKIQAGYKGMRARRGLR----TSASIK---------AEDEIDIDMEDPDEERAATKIQAGYRGMRTRRE-------------MQGRNEIV-------EPAEDP-IDIDLTDPEVDRAATKIQAGYKGMRERKKLRE-----------------------NQ-----------------------ALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDI------VDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEE-----FPEESPETGLTED--------------QAATKIQAGYRGMRARKEIVPL-------NLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAM----------------------DIDLTDPEVERAATKIQAGYKGMRTRRELK----------IPYPEEAIHM-----ADPE---------------------MEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSK------------MDPQNELVE--GELDHAASAAEVDAYGEP-------INIDMTAPDMEQAATKI----QAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEV----EAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQLRQNRDLMDRDEDSQTVISDIQETLIVATHVDALGAIPFDMHPSAVDGPEDD-EDEDKDKKGKNRQSSAGSEREDETFIAVGEESPSKVMRQDSVMRSSSLDASSSSLKPKNDLPP---IMLYHYSRSYNSQKVVIYLRERGIQYSGYHVDLQRNEHLSNWFLDLNPKGEVPVMKFDNQVI-VDSSRIIHHLEAHVPGETYPLLVPLSSETLLYQQYLYFAALLDNIPLNQVVQG---VAFHPELSLAQGRSEPYDEPQVFEKIQQTAIRRGLVLRQTAESNEKAASDLRKRASQFEKEILPNVKDRSAFEDTLDQVESILEKVEAQLSTTMHLEDECQGWLLGSSFSALDITLGVSLNRLAQLGLQPRFWLGDPARPHLLQFLEEIQKRPHFQCNELGLGQARVDLSPLDSALEVCEAGNGNPATLMRTQLTEGNSDSVDVWQELKNGIPDENVSQVDDEEPEEEVTWKSLW 1698
DIDL D DV AATKIQAG+RG KAR++++ ++ DE + + + + ++ D D+ LE D E +E+DID DP+V AATKIQAG+RG+K R+ ++ P S E ID D +VE+A+T I + + + K EP PE DID DP++ QAATKIQAG+RG++ R+ +K + D+ P+ E+DIDL DP+V++AATKIQAGFRG+KAR++ K + KDE+ D E+I P IDIDL DP+VE+AATKIQAG++G++ R+E++ + P +D D + E AATKIQAG+RG + RK ++ + E E +DIDL DP+VE+AATKIQAG++G++AR+E+++ ++E D+ +DIDL DPEV+ AA KIQAG++G++AR+++K+ D E +P +DIDLTDP++E AATKIQAG++G++AR+ +++ ++E +N D IDIDL DPD+E+AATKIQAG++G++AR+E+++ K+E + TE IDIDL DPEVE+AATKIQAG++G+++R K + E+ E D +IDIDL DP+ AA++IQAG++G + R+ M+E+ A AEQ D +DIDL DP+VE+AAT+IQAG+RG++ RQ +K + E EP+PE + DIDL DPEVE+AATKIQAG++G++AR+ ++ T+ S + E+++DID+ DPD E AATKIQAG+RG++ R+E ++ N +V EPA DP IDIDLTDP+V++AATKIQAG++G++ R+++++ NQ ++IDL DP++ QAATKIQAG+RG +ARK+ V K+ + + N+ + DIDL+DPD+E AATKIQAG++G+K R+ ++ + +E E+ ED QAATKIQAG+RG++AR+E+ + ++E + E EP IDIDL+DP+VE+AATKIQAG++G++ RK +++ EE + +IDLTDP+VE+AATKIQAG++G++ R+E+K PY E+ I +PE +E AATKIQAG++GM+ R+ EV +E + EG++S + L E GE + + + + D Y P I+ + AP++ + + + F +Q R + E F D+N+ E E AA+ IQAG+ GM+ R+ L ++S+ EV + E+ +TIS K G AR +GAIPF++H S PED+ E + DK NR SS E I + + R+ S++++ D L ++ P I++Y +S + +YL E+ +++ V +R HL+ FL++NP+G +P M FD ++ S +I H +E H+P YP L+P ++ LYQ+YLYF++ D+I + + G + +L++ + SE DE + Q + +++ L K +K L + ++ D L++V+ +L EA+ + + WLLGS+F+A+DI LGV +N L ++GL W G + +L F + + R Q G+ LDS+ V E+G P + T++ D + L N E V + E+ TW +LW
Sbjct: 415 DIDLTDSDVEKAATKIQAGFRGIKARQEVQKMKEESNYLLLDEQENTGETESLLEPEVDVDQIYANESMQSLETDLALKTEPNSEIDIDFTDPDVEQAATKIQAGFRGIKARQEVKKMQDERKAVHHVDIERATTEETDALIQSEPESNPEADIDFTDLDVEQASTMIN----NIGETEYILKNEPEPETEIDIDDTDPDVEQAATKIQAGFRGIKARQEVKKMKDESKLVFPPEEVNIVEFESILENKPAPDTEIDIDLTDPDVEQAATKIQAGFRGIKARQEVK-KMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVQKMKDDKVIVTQTEEVDESKPTAYPMTEPDIDIDLTDPNTEHAATKIQAGFRGSKARKEVQKMKDEIKSVFPDEEVYDIQTEPLHIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKEFATERDTITEPESANSENRDNNVIDIDLTDPEVQEAAIKIQAGFRGIKARQEVKKIKDTNESVQPEETEMENETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKIRDESKLVIPPDEENTVESESILVNEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDDKVINEQTEEVDDSKLIENPDIDIDLTDPKTEHAATKIQAGFRGSKARQEVQKMKEEIKSALPAEQVDKVETEPLLIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQKVKKMKDEKEIIQITKQEQISEQEPIPEQELADIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDTNESAQPEEIEMETETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKMKDECKSIIPLEEENIVVIESILENEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDKKESLQITEQALIAEQEPITNQDLINAQEPITELEPTDTEKEDKDVIDIDLTDPEIEQAATKIQAGFRGIKARKE-------VQKMKDSNELIQPINQEETELETETEAKADIDLTDPDMELAATKIQAGFRGIKARQEVKKMQDEKEFVMITEQESAAPEIEDKNDVDIDFSDPDLEQAATKIQAGFRGLKARQEVKKMKGEEEFVQITEQEQADLKNELESEPN-IDIDLTDPDVEQAATKIQAGFRGIKARKEVKKIKEESSTFLPSKQGDESKVDPLNELNYDPEINIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDESNPIISPYQEQIIKTESLLNVNPEPTPEVDSDLDCEAVVDNDNQELEDAATKIQAGFKGMKARK--------EVKQRKEDAKAKDDEGNMSNATEDVSAVGEEETEEGTVLGEPLGEKNDSYPSQD-DGYISPLLSESTDIHTAVLAPELSENMASLPSEEEHSFDTIQ-------RSSSVESGFSNDVNNDEKLSKEEKAATLIQAGFRGMKGRKQLK------NILKSSTEVPVN---EEHDTISLREK-----SGKVAR--------------------------------------VGAIPFELHHSF--NPEDETEIREGDKDSFNRLSS-------EHTIDLN------INRESSIVKT---DEKKFELDNNDEFDPNTSIIVYMDESQPSSNVLELYLFEKKVKFVKTQVSRKRKNHLTPEFLEINPEGTLPTMLFDKNIMKAGSMKIAHFIEEHLPITEYPALIPCTNPPPLYQKYLYFSSQFDDIDMTALEIGSKLLTLENQLTV-KDISEEIDE-----------------MVQLSGADDILEPHLSKVIETTKKTWLSGTE---SYHDLLNKVKQVLNNCEAEFAES------TSSWLLGSTFTAVDIQLGVLINHLEKIGLFEIIWDG---KEYLQCFWKSFKARQSVQKILFKSGEN------LDSSENVNESGAVRPGSGDMTRVD-------DEFLTLDNSTEKEEV-----QSRREDRTWYNLW 2337 HSP 2 Score: 722.62 bits (1864), Expect = 0.000e+0 Identity = 546/1567 (34.84%), Postives = 831/1567 (53.03%), Query Frame = 0
Query: 79 TEVENLSGDDIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRP-----------------------ASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDD------------PEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENI---------------------PSIDIDLDDPEVEKAATKIQ-AGYKGMRVRREMRGPALALPSNSMDADDSSQEL--AATKIQAGYRGMRVRKGLKATQAEN------------------------EEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEE------------------PNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEE-----------------PANEV-IDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINA---DEAI---------------DIDLEDPDMERAATKIQAGYKGMRARKELRQNKEEL-------------------KDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMRE--------------KAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMR--------------EKAAEAEQ---------GDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDT-----------------FDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDE---------------IDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPA-----------------EDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLR--------------------------ENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEF---------------------PEESPETG--LTE---DQAATKIQAGYRGMRARKEIV---------------------PLNLSEMTRDGDPF----PEEGEPE---AIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEE----QP-------------DAMDIDLTDPEVERAATKIQAGYKGMRTRRELK----------IPYPEEA-----------IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEP-INIDMTAPDMEQAATKIQAGFRGMQARKGI--------------QGRVAKAEDA--------FDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVES-------NEEVIIGSYPE---------DDETI---------SAATKIQAGFKGMKARRDLSQLRQN 1237
T V+ L DIDL D +V AATKIQAG+RG KAR+++K +++ + +T + E +EL + +++ +DIDL DP V AATKIQAG+RG+K R+ ++ A+ ++IDL D +VEKAATKIQAGF+G++ R++++K + EE + LD+ PE++ + ++T +K +P E+DID DP+V++AATKIQAGFRG+KAR++ K + +DE + ++ P DID D +VE+A+T I G ++ E P +D DD+ ++ AATKIQAG+RG++ R+ +K + E+ + IDIDL DP+VE+AATKIQAG++G++AR+E+++ ++E P +DIDL DP+VE+AATKIQAG++G++AR+++++ +++ P E IDIDLTDP E AATKIQAG++G +AR+ +++ ++EI + DE + DIDL DPD+E+AATKIQAG++G++AR+E+++ K+E +D IDIDL DPEV+ AA KIQAG++G+++R+ +++ E ++++DIDL DP++ +AA++IQAG++G++ R+ ++ E E+E +IDIDL DP+VE+AAT+IQAG+RG++ RQ +K ++ E V D T DIDL DPEVE+AATKIQAG++G++AR+ ++ K +E IDID+ DP E AATKIQAG+RG + R+E+Q E ++ A E +DIDLTDP+V++AATKIQAG++G++ R+K++ E ++IDL DP++ QAATKIQAG+RG +AR++ ++ +N P+ ++ E VDIDL+DPD+E AATKIQAG++G+K R+ ++ + +E P PE LT+ +QAATKIQAG+RG++AR+E+ P+ ++ +P P + E E IDIDL+DPE+E+AATKIQAG++G++ RK +++ + QP DIDLTDP++E AATKIQAG++G++ R+E+K I E A I +DP++EQAATKIQAG+RG++ R+ +K + E V +IT + ++ D +NEL EP I+ID+T PD+EQAATKIQAGFRG++ARK + QG +K + +IDL DP+VE AA+KIQAG+ G++ R+++ + + + S E ++ PE D E + AATKIQAGFKGMKAR+++ Q +++
Sbjct: 293 TFVKELDKIDIDLEDIEVQQAATKIQAGFRGHKARKELK--------KNVNVD---ETTEQEQNTIELLN---DDSNMDIDLMDPNVEQAATKIQAGFRGIKARQEVKKLKEQREIVEDVESFQNEDLLMEKAAEVDIDLTDSDVEKAATKIQAGFRGIKARQEVQK---MKEESNYLLLDEQENTGETESLLEPEVDVDQIYANESMQSLETDLALKT---EPNSEIDIDFTDPDVEQAATKIQAGFRGIKARQEVK-KMQDERKAVHHVDIERATTEETDALIQSEPESNPEADIDFTDLDVEQASTMINNIGETEYILKNEPE------PETEIDIDDTDPDVEQAATKIQAGFRGIKARQEVKKMKDESKLVFPPEEVNIVEFESILENKPAPDTEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVQKMKDDKVIVTQTEEVDESKPTAYPMTEPDIDIDLTDPNTEHAATKIQAGFRGSKARKEVQKMKDEIKSVFPDEEVYDIQTEPLHIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKEFATERDTITEPESANSENRDNNVIDIDLTDPEVQEAAIKIQAGFRGIKARQEVKKIKDTNESVQPEETEMENETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKIRDESKLVIPPDEENTVESESILVNEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDDKVINEQTEEVDDSKLIENPDIDIDLTDPKTEHAATKIQAGFRGSKARQEVQKMKEEIKSALPAEQVDKVETEPLLIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQKVKKMKDEKEIIQITKQEQISEQEPIPEQELADIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKD--TNESAQPEEIEMETETEEK--VDIDLTDPDMELAATKIQAGFRGIKARQEVKKMKDECKSIIPLEEENIVVIESILENEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDKKESLQITEQALIAEQEPITNQDLINAQEPITELEPTDTEKEDKDVIDIDLTDPEIEQAATKIQAGFRGIKARKEVQKMKDSNELIQPINQEETELETETEAKADIDLTDPDMELAATKIQAGFRGIKARQEVKKMQDEKEFVMITEQESAAPEIEDKNDVDIDFSDPDLEQAATKIQAGFRGLKARQEVKKMKGEEEFV--QITEQEQA-------DLKNEL----------------ESEPNIDIDLTDPDVEQAATKIQAGFRGIKARKEVKKIKEESSTFLPSKQGDESKVDPLNELNYDPEINIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDESNPIISPYQEQIIKTESLLNVNPEPTPEVDSDLDCEAVVDNDNQELEDAATKIQAGFKGMKARKEVKQRKED 1803 HSP 3 Score: 68.9366 bits (167), Expect = 1.337e-7 Identity = 29/43 (67.44%), Postives = 37/43 (86.05%), Query Frame = 0
Query: 4 KPLFIRLNLPTGLEESLEGLAKEVLRSQPSNILHFSAEYFENQ 46
+PLFIRL LP GLE++LEGLA+EVLR QP NIL F+A++F+ Q
Sbjct: 7 RPLFIRLTLPAGLEDALEGLAREVLRHQPENILEFAADHFDLQ 49
BLAST of sperm surface protein sp17 vs. nr
Match: gi|1325261168|ref|XP_023330585.1| (kinesin-related protein 4-like isoform X2 [Eurytemora affinis]) HSP 1 Score: 789.26 bits (2037), Expect = 0.000e+0 Identity = 672/2069 (32.48%), Postives = 1023/2069 (49.44%), Query Frame = 0
Query: 88 DIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRD-------DESKQSIKIQMPVQTMDDEDE--------GLELDDPEAEE--AELDIDLEDPEVANAATKIQAGYRGMKTRKHLR-----------------------------PASPIE--IDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMK-VHDKQ-----------------------PEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEE---DSQENI------------PSIDIDLDDPEVEKAATKIQAGYKGMRVRREMR---------------------GPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAE--------------------NEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPN-------------------DSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKR--DNEE--EPANEV--------IDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEE-------------------INA---DEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEE---LKDTE---------------AIDIDLEDPEVERAATKIQAGYKGMRSR---------KSMREKAAEHQD-------EIDIDLEDPEVLMAASRIQAGYKGMRTRK---SMREK---AAEAEQGD-------------IDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPV----------------EAEPVPEDDT--FDIDLEDPEVERAATKIQAGYKGMRARRGLR----TSASIK---------AEDEIDIDMEDPDEERAATKIQAGYRGMRTRRE-------------MQGRNEIV-------EPAEDP-IDIDLTDPEVDRAATKIQAGYKGMRERKKLRE-----------------------NQ-----------------------ALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDI------VDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEE-----FPEESPETGLTED--------------QAATKIQAGYRGMRARKEIVPL-------NLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAM----------------------DIDLTDPEVERAATKIQAGYKGMRTRRELK----------IPYPEEAIHM-----ADPE---------------------MEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSK------------MDPQNELVE--GELDHAASAAEVDAYGEPINIDMT------APDMEQAATKI----QAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEV----EAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQLRQNRDLMDRDEDSQTVISDIQETLIVATHVDALGAIPFDMHPSAVDGPEDD-EDEDKDKKGKNRQSSAGSEREDETFIAVGEESPSKVMRQDSVMRSSSLDASSSSLKPKNDLPP---IMLYHYSRSYNSQKVVIYLRERGIQYSGYHVDLQRNEHLSNWFLDLNPKGEVPVMKFDNQVI-VDSSRIIHHLEAHVPGETYPLLVPLSSETLLYQQYLYFAALLDNIPLNQVVQG---VAFHPELSLAQGRSEPYDEPQVFEKIQQTAIRRGLVLRQTAESNEKAASDLRKRASQFEKEILPNVKDRSAFEDTLDQVESILEKVEAQLSTTMHLEDECQGWLLGSSFSALDITLGVSLNRLAQLGLQPRFWLGDPARPHLLQFLEEIQKRPHFQCNELGLGQARVDLSPLDSALEVCEAGNGNPATLMRTQLTEGNSDSVDVWQELKNGIPDENVSQVDDEEPEEEVTWKSLW 1698
DIDL D DV AATKIQAG+RG KAR++++ ++ DE + + + + ++ D D+ LE D E +E+DID DP+V AATKIQAG+RG+K R+ ++ P S E ID D +VE+A+T I + + + K EP PE DID DP++ QAATKIQAG+RG++ R+ +K + D+ P+ E+DIDL DP+V++AATKIQAGFRG+KAR++ K + KDE+ D E+I P IDIDL DP+VE+AATKIQAG++G++ R+E++ + P +D D + E AATKIQAG+RG + RK ++ + E E +DIDL DP+VE+AATKIQAG++G++AR+E+++ ++E D+ +DIDL DPEV+ AA KIQAG++G++AR+++K+ D E +P +DIDLTDP++E AATKIQAG++G++AR+ +++ ++E +N D IDIDL DPD+E+AATKIQAG++G++AR+E+++ K+E + TE IDIDL DPEVE+AATKIQAG++G+++R K + E+ E D +IDIDL DP+ AA++IQAG++G + R+ M+E+ A AEQ D +DIDL DP+VE+AAT+IQAG+RG++ RQ +K + E EP+PE + DIDL DPEVE+AATKIQAG++G++AR+ ++ T+ S + E+++DID+ DPD E AATKIQAG+RG++ R+E ++ N +V EPA DP IDIDLTDP+V++AATKIQAG++G++ R+++++ NQ ++IDL DP++ QAATKIQAG+RG +ARK+ V K+ + + N+ + DIDL+DPD+E AATKIQAG++G+K R+ ++ + +E E+ ED QAATKIQAG+RG++AR+E+ + ++E + E EP IDIDL+DP+VE+AATKIQAG++G++ RK +++ EE + +IDLTDP+VE+AATKIQAG++G++ R+E+K PY E+ I +PE +E AATKIQAG++GM+ R+ EV +E + EG++S + L E GE + + + + D Y P+ + T AP++ + + + F +Q R + E F D+N+ E E AA+ IQAG+ GM+ R+ L ++S+ EV + E+ +TIS K G AR +GAIPF++H S PED+ E + DK NR SS E I + + R+ S++++ D L ++ P I++Y +S + +YL E+ +++ V +R HL+ FL++NP+G +P M FD ++ S +I H +E H+P YP L+P ++ LYQ+YLYF++ D+I + + G + +L++ + SE DE + Q + +++ L K +K L + ++ D L++V+ +L EA+ + + WLLGS+F+A+DI LGV +N L ++GL W G + +L F + + R Q G+ LDS+ V E+G P + T++ D + L N E V + E+ TW +LW
Sbjct: 415 DIDLTDSDVEKAATKIQAGFRGIKARQEVQKMKEESNYLLLDEQENTGETESLLEPEVDVDQIYANESMQSLETDLALKTEPNSEIDIDFTDPDVEQAATKIQAGFRGIKARQEVKKMQDERKAVHHVDIERATTEETDALIQSEPESNPEADIDFTDLDVEQASTMIN----NIGETEYILKNEPEPETEIDIDDTDPDVEQAATKIQAGFRGIKARQEVKKMKDESKLVFPPEEVNIVEFESILENKPAPDTEIDIDLTDPDVEQAATKIQAGFRGIKARQEVK-KMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVQKMKDDKVIVTQTEEVDESKPTAYPMTEPDIDIDLTDPNTEHAATKIQAGFRGSKARKEVQKMKDEIKSVFPDEEVYDIQTEPLHIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKEFATERDTITEPESANSENRDNNVIDIDLTDPEVQEAAIKIQAGFRGIKARQEVKKIKDTNESVQPEETEMENETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKIRDESKLVIPPDEENTVESESILVNEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDDKVINEQTEEVDDSKLIENPDIDIDLTDPKTEHAATKIQAGFRGSKARQEVQKMKEEIKSALPAEQVDKVETEPLLIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQKVKKMKDEKEIIQITKQEQISEQEPIPEQELADIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDTNESAQPEEIEMETETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKMKDECKSIIPLEEENIVVIESILENEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDKKESLQITEQALIAEQEPITNQDLINAQEPITELEPTDTEKEDKDVIDIDLTDPEIEQAATKIQAGFRGIKARKE-------VQKMKDSNELIQPINQEETELETETEAKADIDLTDPDMELAATKIQAGFRGIKARQEVKKMQDEKEFVMITEQESAAPEIEDKNDVDIDFSDPDLEQAATKIQAGFRGLKARQEVKKMKGEEEFVQITEQEQADLKNELESEPN-IDIDLTDPDVEQAATKIQAGFRGIKARKEVKKIKEESSTFLPSKQGDESKVDPLNELNYDPEINIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDESNPIISPYQEQIIKTESLLNVNPEPTPEVDSDLDCEAVVDNDNQELEDAATKIQAGFKGMKARK--------EVKQRKEDAKAKDDEGNMSNATEDVSAVGEEETEEGTVLGEPLGEKNDSYPSQD-DGYISPLLSESTDIHTVLAPELSENMASLPSEEEHSFDTIQ-------RSSSVESGFSNDVNNDEKLSKEEKAATLIQAGFRGMKGRKQLK------NILKSSTEVPVN---EEHDTISLREK-----SGKVAR--------------------------------------VGAIPFELHHSF--NPEDETEIREGDKDSFNRLSS-------EHTIDLN------INRESSIVKT---DEKKFELDNNDEFDPNTSIIVYMDESQPSSNVLELYLFEKKVKFVKTQVSRKRKNHLTPEFLEINPEGTLPTMLFDKNIMKAGSMKIAHFIEEHLPITEYPALIPCTNPPPLYQKYLYFSSQFDDIDMTALEIGSKLLTLENQLTV-KDISEEIDE-----------------MVQLSGADDILEPHLSKVIETTKKTWLSGTE---SYHDLLNKVKQVLNNCEAEFAES------TSSWLLGSTFTAVDIQLGVLINHLEKIGLFEIIWDG---KEYLQCFWKSFKARQSVQKILFKSGEN------LDSSENVNESGAVRPGSGDMTRVD-------DEFLTLDNSTEKEEV-----QSRREDRTWYNLW 2336 HSP 2 Score: 723.005 bits (1865), Expect = 0.000e+0 Identity = 546/1567 (34.84%), Postives = 831/1567 (53.03%), Query Frame = 0
Query: 79 TEVENLSGDDIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRP-----------------------ASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDD------------PEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENI---------------------PSIDIDLDDPEVEKAATKIQ-AGYKGMRVRREMRGPALALPSNSMDADDSSQEL--AATKIQAGYRGMRVRKGLKATQAEN------------------------EEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEE------------------PNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEE-----------------PANEV-IDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINA---DEAI---------------DIDLEDPDMERAATKIQAGYKGMRARKELRQNKEEL-------------------KDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMRE--------------KAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMR--------------EKAAEAEQ---------GDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDT-----------------FDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDE---------------IDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPA-----------------EDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLR--------------------------ENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEF---------------------PEESPETG--LTE---DQAATKIQAGYRGMRARKEIV---------------------PLNLSEMTRDGDPF----PEEGEPE---AIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEE----QP-------------DAMDIDLTDPEVERAATKIQAGYKGMRTRRELK----------IPYPEEA-----------IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEP-INIDMTAPDMEQAATKIQAGFRGMQARKGI--------------QGRVAKAEDA--------FDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVES-------NEEVIIGSYPE---------DDETI---------SAATKIQAGFKGMKARRDLSQLRQN 1237
T V+ L DIDL D +V AATKIQAG+RG KAR+++K +++ + +T + E +EL + +++ +DIDL DP V AATKIQAG+RG+K R+ ++ A+ ++IDL D +VEKAATKIQAGF+G++ R++++K + EE + LD+ PE++ + ++T +K +P E+DID DP+V++AATKIQAGFRG+KAR++ K + +DE + ++ P DID D +VE+A+T I G ++ E P +D DD+ ++ AATKIQAG+RG++ R+ +K + E+ + IDIDL DP+VE+AATKIQAG++G++AR+E+++ ++E P +DIDL DP+VE+AATKIQAG++G++AR+++++ +++ P E IDIDLTDP E AATKIQAG++G +AR+ +++ ++EI + DE + DIDL DPD+E+AATKIQAG++G++AR+E+++ K+E +D IDIDL DPEV+ AA KIQAG++G+++R+ +++ E ++++DIDL DP++ +AA++IQAG++G++ R+ ++ E E+E +IDIDL DP+VE+AAT+IQAG+RG++ RQ +K ++ E V D T DIDL DPEVE+AATKIQAG++G++AR+ ++ K +E IDID+ DP E AATKIQAG+RG + R+E+Q E ++ A E +DIDLTDP+V++AATKIQAG++G++ R+K++ E ++IDL DP++ QAATKIQAG+RG +AR++ ++ +N P+ ++ E VDIDL+DPD+E AATKIQAG++G+K R+ ++ + +E P PE LT+ +QAATKIQAG+RG++AR+E+ P+ ++ +P P + E E IDIDL+DPE+E+AATKIQAG++G++ RK +++ + QP DIDLTDP++E AATKIQAG++G++ R+E+K I E A I +DP++EQAATKIQAG+RG++ R+ +K + E V +IT + ++ D +NEL EP I+ID+T PD+EQAATKIQAGFRG++ARK + QG +K + +IDL DP+VE AA+KIQAG+ G++ R+++ + + + S E ++ PE D E + AATKIQAGFKGMKAR+++ Q +++
Sbjct: 293 TFVKELDKIDIDLEDIEVQQAATKIQAGFRGHKARKELK--------KNVNVD---ETTEQEQNTIELLN---DDSNMDIDLMDPNVEQAATKIQAGFRGIKARQEVKKLKEQREIVEDVESFQNEDLLMEKAAEVDIDLTDSDVEKAATKIQAGFRGIKARQEVQK---MKEESNYLLLDEQENTGETESLLEPEVDVDQIYANESMQSLETDLALKT---EPNSEIDIDFTDPDVEQAATKIQAGFRGIKARQEVK-KMQDERKAVHHVDIERATTEETDALIQSEPESNPEADIDFTDLDVEQASTMINNIGETEYILKNEPE------PETEIDIDDTDPDVEQAATKIQAGFRGIKARQEVKKMKDESKLVFPPEEVNIVEFESILENKPAPDTEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVQKMKDDKVIVTQTEEVDESKPTAYPMTEPDIDIDLTDPNTEHAATKIQAGFRGSKARKEVQKMKDEIKSVFPDEEVYDIQTEPLHIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKEFATERDTITEPESANSENRDNNVIDIDLTDPEVQEAAIKIQAGFRGIKARQEVKKIKDTNESVQPEETEMENETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKIRDESKLVIPPDEENTVESESILVNEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDDKVINEQTEEVDDSKLIENPDIDIDLTDPKTEHAATKIQAGFRGSKARQEVQKMKEEIKSALPAEQVDKVETEPLLIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQKVKKMKDEKEIIQITKQEQISEQEPIPEQELADIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKD--TNESAQPEEIEMETETEEK--VDIDLTDPDMELAATKIQAGFRGIKARQEVKKMKDECKSIIPLEEENIVVIESILENEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDKKESLQITEQALIAEQEPITNQDLINAQEPITELEPTDTEKEDKDVIDIDLTDPEIEQAATKIQAGFRGIKARKEVQKMKDSNELIQPINQEETELETETEAKADIDLTDPDMELAATKIQAGFRGIKARQEVKKMQDEKEFVMITEQESAAPEIEDKNDVDIDFSDPDLEQAATKIQAGFRGLKARQEVKKMKGEEEFV--QITEQEQA-------DLKNEL----------------ESEPNIDIDLTDPDVEQAATKIQAGFRGIKARKEVKKIKEESSTFLPSKQGDESKVDPLNELNYDPEINIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDESNPIISPYQEQIIKTESLLNVNPEPTPEVDSDLDCEAVVDNDNQELEDAATKIQAGFKGMKARKEVKQRKED 1803 HSP 3 Score: 68.9366 bits (167), Expect = 1.337e-7 Identity = 29/43 (67.44%), Postives = 37/43 (86.05%), Query Frame = 0
Query: 4 KPLFIRLNLPTGLEESLEGLAKEVLRSQPSNILHFSAEYFENQ 46
+PLFIRL LP GLE++LEGLA+EVLR QP NIL F+A++F+ Q
Sbjct: 7 RPLFIRLTLPAGLEDALEGLAREVLRHQPENILEFAADHFDLQ 49
BLAST of sperm surface protein sp17 vs. nr
Match: gi|1325261174|ref|XP_023330594.1| (kinesin-related protein 4-like isoform X3 [Eurytemora affinis]) HSP 1 Score: 786.563 bits (2030), Expect = 0.000e+0 Identity = 668/2053 (32.54%), Postives = 1008/2053 (49.10%), Query Frame = 0
Query: 88 DIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLR-----------------------------PASPIE--IDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMK-----------------------VHDK-QPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEE---DSQENI------------PSIDIDLDDPEVEKAATKIQAGYKGMRVRREMR---------------------GPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAE--------------------NEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPN-------------------DSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKR--DNEE--EPANEV--------IDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEE-------------------INA---DEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEE---LKDTE---------------AIDIDLEDPEVERAATKIQAGYKGMRSR---------KSMREKAAEHQD-------EIDIDLEDPEVLMAASRIQAGYKGMRTRK---SMREK---AAEAEQGD-------------IDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPV----------------EAEPVPEDDT--FDIDLEDPEVERAATKIQAGYKGMRARRGLR----TSASIK---------AEDEIDIDMEDPDEERAATKIQAGYRGMRTRRE-------------MQGRNEIV-------EPAEDP-IDIDLTDPEVDRAATKIQAGYKGMRERKKLRE-----------------------NQ-----------------------ALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDI------VDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEE-----FPEESPETGLTED--------------QAATKIQAGYRGMRARKEIVPL-------NLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQPDAM----------------------DIDLTDPEVERAATKIQAGYKGMRTRRELK----------IPYPEEAIHM-----ADPE---------------------MEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSK------------MDPQNELVE--GELDHAASAAEVDAYGEP-------INIDMTAPDMEQAATKI----QAGFRGMQARKGIQGRVAKAEDAFDIDLNDPEV----EAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQLRQNRDLMDRDEDSQTVISDIQETLIVATHVDALGAIPFDMHPSAVDGPEDD-EDEDKDKKGKNRQSSAGSEREDETFIAVGEESPSKVMRQDSVMRSSSLDASSSSLKPKNDLPP---IMLYHYSRSYNSQKVVIYLRERGIQYSGYHVDLQRNEHLSNWFLDLNPKGEVPVMKFDNQVI-VDSSRIIHHLEAHVPGETYPLLVPLSSETLLYQQYLYFAALLDNIPLNQVVQG---VAFHPELSLAQGRSEPYDEPQVFEKIQQTAIRRGLVLRQTAESNEKAASDLRKRASQFEKEILPNVKDRSAFEDTLDQVESILEKVEAQLSTTMHLEDECQGWLLGSSFSALDITLGVSLNRLAQLGLQPRFWLGDPARPHLLQFLEEIQKRPHFQCNELGLGQARVDLSPLDSALEVCEAGNGNPATLMRTQLTEGNSDSVDVWQELKNGIPDENVSQVDDEEPEEEVTWKSLW 1698
DIDL D DV AATKIQAG+RG KAR++ VQ M +E L LD+ E DP+V AATKIQAG+RG+K R+ ++ P S E ID D +VE+A+T I + + + K EP PE DID DP++ QAATKIQAG+RG++ R+ +K + +K P+ E+DIDL DP+V++AATKIQAGFRG+KAR++ K + KDE+ D E+I P IDIDL DP+VE+AATKIQAG++G++ R+E++ + P +D D + E AATKIQAG+RG + RK ++ + E E +DIDL DP+VE+AATKIQAG++G++AR+E+++ ++E D+ +DIDL DPEV+ AA KIQAG++G++AR+++K+ D E +P +DIDLTDP++E AATKIQAG++G++AR+ +++ ++E +N D IDIDL DPD+E+AATKIQAG++G++AR+E+++ K+E + TE IDIDL DPEVE+AATKIQAG++G+++R K + E+ E D +IDIDL DP+ AA++IQAG++G + R+ M+E+ A AEQ D +DIDL DP+VE+AAT+IQAG+RG++ RQ +K + E EP+PE + DIDL DPEVE+AATKIQAG++G++AR+ ++ T+ S + E+++DID+ DPD E AATKIQAG+RG++ R+E ++ N +V EPA DP IDIDLTDP+V++AATKIQAG++G++ R+++++ NQ ++IDL DP++ QAATKIQAG+RG +ARK+ V K+ + + N+ + DIDL+DPD+E AATKIQAG++G+K R+ ++ + +E E+ ED QAATKIQAG+RG++AR+E+ + ++E + E EP IDIDL+DP+VE+AATKIQAG++G++ RK +++ EE + +IDLTDP+VE+AATKIQAG++G++ R+E+K PY E+ I +PE +E AATKIQAG++GM+ R+ EV +E + EG++S + L E GE + + + + D Y P I+ + AP++ + + + F +Q R + E F D+N+ E E AA+ IQAG+ GM+ R+ L ++S+ EV + E+ +TIS K G AR +GAIPF++H S PED+ E + DK NR SS E I + + R+ S++++ D L ++ P I++Y +S + +YL E+ +++ V +R HL+ FL++NP+G +P M FD ++ S +I H +E H+P YP L+P ++ LYQ+YLYF++ D+I + + G + +L++ + SE DE + Q + +++ L K +K L + ++ D L++V+ +L EA+ + + WLLGS+F+A+DI LGV +N L ++GL W G + +L F + + R Q G+ LDS+ V E+G P + T++ D + L N E V + E+ TW +LW
Sbjct: 415 DIDLTDSDVEKAATKIQAGFRGIKARQE------------------VQKMKEESNYLLLDEQENT---------DPDVEQAATKIQAGFRGIKARQEVKKMQDERKAVHHVDIERATTEETDALIQSEPESNPEADIDFTDLDVEQASTMIN----NIGETEYILKNEPEPETEIDIDDTDPDVEQAATKIQAGFRGIKARQEVKKMKDESKLVFPPEEVNIVEFESILENKPAPDTEIDIDLTDPDVEQAATKIQAGFRGIKARQEVK-KMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVQKMKDDKVIVTQTEEVDESKPTAYPMTEPDIDIDLTDPNTEHAATKIQAGFRGSKARKEVQKMKDEIKSVFPDEEVYDIQTEPLHIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKEFATERDTITEPESANSENRDNNVIDIDLTDPEVQEAAIKIQAGFRGIKARQEVKKIKDTNESVQPEETEMENETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKIRDESKLVIPPDEENTVESESILVNEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDDKVINEQTEEVDDSKLIENPDIDIDLTDPKTEHAATKIQAGFRGSKARQEVQKMKEEIKSALPAEQVDKVETEPLLIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQKVKKMKDEKEIIQITKQEQISEQEPIPEQELADIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDTNESAQPEEIEMETETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKMKDECKSIIPLEEENIVVIESILENEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDKKESLQITEQALIAEQEPITNQDLINAQEPITELEPTDTEKEDKDVIDIDLTDPEIEQAATKIQAGFRGIKARKE-------VQKMKDSNELIQPINQEETELETETEAKADIDLTDPDMELAATKIQAGFRGIKARQEVKKMQDEKEFVMITEQESAAPEIEDKNDVDIDFSDPDLEQAATKIQAGFRGLKARQEVKKMKGEEEFVQITEQEQADLKNELESEPN-IDIDLTDPDVEQAATKIQAGFRGIKARKEVKKIKEESSTFLPSKQGDESKVDPLNELNYDPEINIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDESNPIISPYQEQIIKTESLLNVNPEPTPEVDSDLDCEAVVDNDNQELEDAATKIQAGFKGMKARK--------EVKQRKEDAKAKDDEGNMSNATEDVSAVGEEETEEGTVLGEPLGEKNDSYPSQD-DGYISPLLSESTDIHTAVLAPELSENMASLPSEEEHSFDTIQ-------RSSSVESGFSNDVNNDEKLSKEEKAATLIQAGFRGMKGRKQLK------NILKSSTEVPVN---EEHDTISLREK-----SGKVAR--------------------------------------VGAIPFELHHSF--NPEDETEIREGDKDSFNRLSS-------EHTIDLN------INRESSIVKT---DEKKFELDNNDEFDPNTSIIVYMDESQPSSNVLELYLFEKKVKFVKTQVSRKRKNHLTPEFLEINPEGTLPTMLFDKNIMKAGSMKIAHFIEEHLPITEYPALIPCTNPPPLYQKYLYFSSQFDDIDMTALEIGSKLLTLENQLTV-KDISEEIDE-----------------MVQLSGADDILEPHLSKVIETTKKTWLSGTE---SYHDLLNKVKQVLNNCEAEFAES------TSSWLLGSTFTAVDIQLGVLINHLEKIGLFEIIWDG---KEYLQCFWKSFKARQSVQKILFKSGEN------LDSSENVNESGAVRPGSGDMTRVD-------DEFLTLDNSTEKEEV-----QSRREDRTWYNLW 2293 HSP 2 Score: 708.368 bits (1827), Expect = 0.000e+0 Identity = 538/1522 (35.35%), Postives = 808/1522 (53.09%), Query Frame = 0
Query: 133 PVQTMDDEDEGL-ELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPA--------------------SPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEP---VPEEL---------------ADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLD-----DPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENI---------------------PSIDIDLDDPEVEKAATKIQ-AGYKGMRVRREMRGPALALPSNSMDADDSSQEL--AATKIQAGYRGMRVRKGLKATQAEN------------------------EEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEE------------------PNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEE-----------------PANEV-IDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINA---DEAI---------------DIDLEDPDMERAATKIQAGYKGMRARKELRQNKEEL-------------------KDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMRE--------------KAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMR--------------EKAAEAEQ---------GDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVEAEPVPEDDT-----------------FDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDE---------------IDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPA-----------------EDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLR--------------------------ENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEF---------------------PEESPETG--LTE---DQAATKIQAGYRGMRARKEIV---------------------PLNLSEMTRDGDPF----PEEGEPE---AIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEE----QP-------------DAMDIDLTDPEVERAATKIQAGYKGMRTRRELK----------IPYPEEA-----------IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEP-INIDMTAPDMEQAATKIQAGFRGMQARKGI--------------QGRVAKAEDA--------FDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVES-------NEEVIIGSYPE---------DDETI---------SAATKIQAGFKGMKARRDLSQLRQN 1237
P QT D D + ELD ++DIDLED EV AATKIQAG+RG K RK L+ S ++IDL DP VE+AATKIQAGF+G++ R++++K + + E++ DIDL D ++ +AATKIQAG+RG++ R+ ++ K E+ + LD DP+V++AATKIQAGFRG+KAR++ K + +DE + ++ P DID D +VE+A+T I G ++ E P +D DD+ ++ AATKIQAG+RG++ R+ +K + E+ + IDIDL DP+VE+AATKIQAG++G++AR+E+++ ++E P +DIDL DP+VE+AATKIQAG++G++AR+++++ +++ P E IDIDLTDP E AATKIQAG++G +AR+ +++ ++EI + DE + DIDL DPD+E+AATKIQAG++G++AR+E+++ K+E +D IDIDL DPEV+ AA KIQAG++G+++R+ +++ E ++++DIDL DP++ +AA++IQAG++G++ R+ ++ E E+E +IDIDL DP+VE+AAT+IQAG+RG++ RQ +K ++ E V D T DIDL DPEVE+AATKIQAG++G++AR+ ++ K +E IDID+ DP E AATKIQAG+RG + R+E+Q E ++ A E +DIDLTDP+V++AATKIQAG++G++ R+K++ E ++IDL DP++ QAATKIQAG+RG +AR++ ++ +N P+ ++ E VDIDL+DPD+E AATKIQAG++G+K R+ ++ + +E P PE LT+ +QAATKIQAG+RG++AR+E+ P+ ++ +P P + E E IDIDL+DPE+E+AATKIQAG++G++ RK +++ + QP DIDLTDP++E AATKIQAG++G++ R+E+K I E A I +DP++EQAATKIQAG+RG++ R+ +K + E V +IT + ++ D +NEL EP I+ID+T PD+EQAATKIQAGFRG++ARK + QG +K + +IDL DP+VE AA+KIQAG+ G++ R+++ + + + S E ++ PE D E + AATKIQAGFKGMKAR+++ Q +++
Sbjct: 284 PTQTTDQPDTFVKELD-------KIDIDLEDIEVQQAATKIQAGFRGHKARKELKKNVNVDETTEQEQNTIELLNDDSNMDIDLMDPNVEQAATKIQAGFRGIKARQEVKKLKEQREIVEDVESFQNEDLLMEKAAEVDIDLTDSDVEKAATKIQAGFRGIKARQEVQ---KMKEESNYLLLDEQENTDPDVEQAATKIQAGFRGIKARQEVK-KMQDERKAVHHVDIERATTEETDALIQSEPESNPEADIDFTDLDVEQASTMINNIGETEYILKNEPE------PETEIDIDDTDPDVEQAATKIQAGFRGIKARQEVKKMKDESKLVFPPEEVNIVEFESILENKPAPDTEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVQKMKDDKVIVTQTEEVDESKPTAYPMTEPDIDIDLTDPNTEHAATKIQAGFRGSKARKEVQKMKDEIKSVFPDEEVYDIQTEPLHIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKEFATERDTITEPESANSENRDNNVIDIDLTDPEVQEAAIKIQAGFRGIKARQEVKKIKDTNESVQPEETEMENETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKIRDESKLVIPPDEENTVESESILVNEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDDKVINEQTEEVDDSKLIENPDIDIDLTDPKTEHAATKIQAGFRGSKARQEVQKMKEEIKSALPAEQVDKVETEPLLIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQKVKKMKDEKEIIQITKQEQISEQEPIPEQELADIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKD--TNESAQPEEIEMETETEEK--VDIDLTDPDMELAATKIQAGFRGIKARQEVKKMKDECKSIIPLEEENIVVIESILENEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDKKESLQITEQALIAEQEPITNQDLINAQEPITELEPTDTEKEDKDVIDIDLTDPEIEQAATKIQAGFRGIKARKEVQKMKDSNELIQPINQEETELETETEAKADIDLTDPDMELAATKIQAGFRGIKARQEVKKMQDEKEFVMITEQESAAPEIEDKNDVDIDFSDPDLEQAATKIQAGFRGLKARQEVKKMKGEEEFV--QITEQEQA-------DLKNEL----------------ESEPNIDIDLTDPDVEQAATKIQAGFRGIKARKEVKKIKEESSTFLPSKQGDESKVDPLNELNYDPEINIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDESNPIISPYQEQIIKTESLLNVNPEPTPEVDSDLDCEAVVDNDNQELEDAATKIQAGFKGMKARKEVKQRKED 1759 HSP 3 Score: 551.206 bits (1419), Expect = 3.883e-160 Identity = 429/1236 (34.71%), Postives = 652/1236 (52.75%), Query Frame = 0
Query: 358 MDADDSSQELAATKIQAGYRGMRVRKGLK-------ATQAE--------NEEGIDIDLDDPEVERAATKIQAGYKGMRARKE---LRQHQEEPNDSEP-------------MDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAI------------------------DIDLEDPDME------------------------------------RAATKIQAGYKGMRARKELRQNKEELK-----------------------DTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEH--------------------QDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMRE-------------------KAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTR---QGMK-------------PVEAEP--VPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRT------------------SASIKAEDE--IDIDMEDPDEERAATKIQAGYRGMRTRRE---MQGRNEIVEP--------AEDPIDIDLTDPEVDRAATKIQAGYKGMRER---KKLRENQAL--------------------------EIDLDDPDLAQAATKIQAGYRGHQARKDYVSRRE---VVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLRALPE-----EFPEESPETGLTED-------------QAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPE----EGEPEAI-----DIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQ-----------------PDA----MDIDLTDPEVERAATKIQAGYKGMRTRREL-KIPYPEEA-----------------IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEP-INIDMTAPDMEQAATKIQAGFRGMQARKGI--------------QGRVAKAE---------------------------DAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIGSYPE----------DDETISAATKIQAGFKGMKARRDLSQLRQNRDLM 1241
+D +D + AATKIQAG+RG + RK LK T+ E ++ +DIDL DP VE+AATKIQAG++G++AR+E L++ +E D E +DIDL D +VE+AATKIQAG++G++AR+++++ EE + + + TDP++EQAATKIQAG++G++AR+ +++ Q+E A + DID D D+E +AATKIQAG++G++AR+E+++ K+E K DTE IDIDL DP+VE+AATKIQAG++G+++R+ +++ E + EIDIDL DP+V AA++IQAG++G++ R+ +++ A + DIDIDL DP E AAT+IQAG+RG + R Q MK ++ EP +PE + DIDL DP+VE+AATKIQAG++G++AR+ ++ SA+ + D IDID+ DP+ + AA KIQAG+RG++ R+E ++ NE V+P E+ +DIDLTDP+++ AATKIQAG++G++ R KK+R+ L +IDL DPD+ QAATKIQAG+RG +AR++ ++ +V ++I +S N E +I DIDL+DP+VE+AATKIQAG++G+K R+ ++ + + E EE ++ L E+ AATKIQAG+RG +AR+E+ + E + P + E EP I DIDL+DP+VE+AATKIQAG++G++ R+ +++ +E+ P+ +DIDLTDPEVE+AATKIQAG++G++ R+E+ K+ E+ I + DP+ME AATKIQAG+RG++ R+ +K + D + I P E +N +V S E + +P I+ID+T PD+EQAATKIQAGFRG++AR+ + Q +A+ E D DIDL DPE+E AA+KIQAG+ G++ R+++ + E ++ + E D + AATKIQAGF+G+KAR+++ +++ ++ +
Sbjct: 303 IDLEDIEVQQAATKIQAGFRGHKARKELKKNVNVDETTEQEQNTIELLNDDSNMDIDLMDPNVEQAATKIQAGFRGIKARQEVKKLKEQREIVEDVESFQNEDLLMEKAAEVDIDLTDSDVEKAATKIQAGFRGIKARQEVQKMKEESNYLLLDEQENTDPDVEQAATKIQAGFRGIKARQEVKKMQDERKAVHHVDIERATTEETDALIQSEPESNPEADIDFTDLDVEQASTMINNIGETEYILKNEPEPETEIDIDDTDPDVEQAATKIQAGFRGIKARQEVKKMKDESKLVFPPEEVNIVEFESILENKPAPDTE-IDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVQKMKDDKVIVTQTEEVDESKPTAYPMTEPDIDIDLTDPNTEHAATKIQAGFRGSKARKEVQKMKDEIKSVFPDEEVYDIQTEPLHIPEPEV-DIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKEFATERDTITEPESANSENRDNNVIDIDLTDPEVQEAAIKIQAGFRGIKARQEVKKIKDTNESVQPEETEMENETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKIRDESKLVIPPDEENTVESESILVNEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDEKVIVDQTEDIDESKLTANPLTEPEI-DIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDDKVINEQTEEVDDSKLIENPDIDIDLTDPKTEHAATKIQAGFRGSKARQEV--QKMKEEIKSALPAEQVDKVETEPLLIPEPEVDIDLTDPDVEQAATKIQAGFRGIKARQKVKKMKDEKEIIQITKQEQISEQEPIPEQELADIDIDLTDPEVEQAATKIQAGFRGIKARQEVKKMKDTNESAQPEEIEMETETEEKVDIDLTDPDMELAATKIQAGFRGIKARQEVKKMKD----ECKSIIPLEE----------ENIVV------IESILENEPAPDPEIDIDLTDPDVEQAATKIQAGFRGIKARQEVKKMKDKKESLQITEQALIAEQEPITNQDLINAQEPITELEPTDTEKEDKDVIDIDLTDPEIEQAATKIQAGFRGIKARKEVQKMKDSNELIQPINQEETELETETEAKADIDLTDPDMELAATKIQAGFRGIKARQEVKKMQDEKEFV 1513
BLAST of sperm surface protein sp17 vs. nr
Match: gi|1205889575|gb|OWF40450.1| (Sperm surface protein Sp17 [Mizuhopecten yessoensis]) HSP 1 Score: 518.079 bits (1333), Expect = 8.810e-154 Identity = 378/994 (38.03%), Postives = 544/994 (54.73%), Query Frame = 0
Query: 9 RLNLPTGLEESLEGLAKEVLRSQPSNILHFSAEYFENQGLPANQDIFDYIALSSDVNPNKYDNAPPLEDGTEVENLSGDDIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEA--EEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPA------------SPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPE--------ELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQP-------------EDEVDIDLDDPEVQEAATKIQAGFRGMKARK-----QAKPE----------AKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQH------QEEPNDSEP-------------MDIDLEDPEVERAATKIQAGYKGMRAR-----KQLKRDN-----------EEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARR----ALRQHQEEIN-------------ADEAIDIDLEDPDMERAATKIQAGYKGMRARK-ELRQNKEE--------------LKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAA------------------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEA---------------EQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ--GMKPVEAEPVP-----------EDDTFDIDLEDPEVERAATKIQAGYKGMRAR--RGLRTSASIKA-------EDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQ-------GRNEIVEPA---------------------EDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLR 802
+L +P G + LEGLA+EVLR+QP NI F A+YF+ ++ D + + Y+N + +D DP AA KIQ YR A++++ R++++ + IQ V+ D + E+ + EA + AE+DI+LEDPEV AA KIQAG++G K RK ++ + ++IDL DPEV KAATKIQAGF+G +TRK+++ +E P+ E DIDL+DPE+ +AATKIQAG++G +TR+ M + KQP D DID +DP+V +AATKIQA F+G + RK Q+ PE + D+ ++ IDIDL DPEVEKAATKIQAGYKG++ RRE++ S QE +T I VR ++ E EE IDIDL DPEVE+AA KIQ+ +KG+RARK + EP+++ P +DIDL DPEVE+AA KIQA +KG+++R K +N ++ P E IDIDL DPE+ +AA KIQ+ +KG+++R+ Q IN ++ IDIDL DP++ +AA KIQ+ +KG+++RK + +Q+ + + E +DIDL DPEVE+AA KIQ+ +KG++SRK +K++ ++EIDIDL DPEV AA +IQA +KG+++RK +K++ + + +IDIDLNDPEVEKAA +IQA ++G+++R+ KPV P E + DIDL DP+VE+AA KIQA +KG+RAR + + +A +A E+EIDID+ DP+ +AA KIQAG+RG +TR+E++ + E +P+ E+P+DIDL DPEV +AATKIQAG+KG + RK ++
Sbjct: 9 KLRVPKGFQNILEGLAREVLRNQPENIYEFGAKYFDQLLRVRDETGHDPAVHGARMEDRFYNN----------DAFRSPGVDSGDPQQQDAALKIQTEYRRHSAKKEVDDMREEDA--ATTIQAGVRGHQDRQKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEM--NGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELK---------------SIQEPKSTSIP-------VRDSMENVPTE-EEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 965 HSP 2 Score: 471.855 bits (1213), Expect = 4.028e-137 Identity = 337/901 (37.40%), Postives = 485/901 (53.83%), Query Frame = 0
Query: 158 IDLEDPEVANAATKIQAGYRGMKTRKHLRPASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEEL----ADIDLDDPEMNQAATKIQAGYRGMQTRRGMK-------VHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIP--SIDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPAL------------ALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQA--ENEEG-IDI------------------DLDDPEVERAATKIQAGYKGMRARKELRQHQEE--------------PNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQ-----LKRDNEEEPAN--------------EVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEIN----------------ADEAIDIDLEDPDMERAATKIQAGYKGMRARK----ELRQNKEELKDT-------------EAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEH-----------------QDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAA-----------------EAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ-GMK------------PVEAEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLR-----------TSASIKAE----DEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGR-------NEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLREN------------------------------------QALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRRE 841
+D DP+ +AA KIQ YR +K E+D D E AAT IQAG +G + R+++ + + L DI+L+DPE+ AA KIQAG++G + R+ +K ++ ++EVDIDL+DPEV +AATKIQAGFRG K RK+ K + EN IDIDL+DPEV KAATKIQAG+KG + R+EM G ++ +D +D AATKIQA ++G +VRK ++ Q+ E + G DI DL DPEVE+AATKIQAGYKG++ R+EL+ QE P + E +DIDL DPEVE+AA KIQ+ +KG+RARK E EP+N E IDIDL DPE+E+AA KIQA +KG+++RR + N +E IDIDL DP++ +AA KIQ+ +KG+++RK + Q+ + + + IDIDL DPEV +AA KIQ+ +KG++SRK +++ + ++E+DIDL DPEV AA +IQ+ +KG+++RK +K++ + +IDIDLNDPEVEKAA +IQA ++G+++R+ G K P +P ++ DIDL DPEVE+AA KIQA +KG+++R+ + +S + A+ +EIDID+ DPD E+AA KIQA ++G+R R+ + + E E+ IDIDL DPE +AA KIQAG++G + RK+++EN + ++IDL+DP++A+AATKIQAG++G++ RKD +E
Sbjct: 79 VDSGDPQQQDAALKIQTEYRRHSAKK--------EVD--DMREEDAATTIQAGVRGHQDRQKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMKGDKE 969 HSP 3 Score: 464.537 bits (1194), Expect = 1.239e-134 Identity = 345/923 (37.38%), Postives = 507/923 (54.93%), Query Frame = 0
Query: 398 IDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLK--RDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQE-----EINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELK------DTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSM-----------REKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMR--EKAAEAEQGDIDI------------------DLNDPEVEKAATRIQAGYRGMRTRQGMKPVE------------AEPVP-EDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSA------------------SIKAE--DEIDIDMEDPDEERAATKIQAGYRGMRTRR-------------EMQGRNEIVE--PAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--KLRENQA----------------------LEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRK--TLRALPEEF---------------PEESPETGLTE---DQAATKIQAGYRGMRARK----EIVPLNLSEMTRDGDPFPEEGEP--EAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE---EQPDA-------------MDIDLTDPEVERAATKIQAGYKGMRTRR--ELKIPYPEEA------------IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMD-PQNELVEGELDHAASAAEVDAYGE-PINIDMTAPDMEQAATKIQAGFRGMQARKGIQG 1146
+D DP+ + AA KIQ Y+ A+KE+ +EE AAT IQAG +G + R+++ ++ E + +DI+L DPE+E AA KIQAG+KG +AR+ +++ +E E +DIDLEDP++ +AATKIQAG++G + RKEL+ +++ K + E IDIDL DPEV +AATKIQAG+KG ++RK M R + D DID DP+V AA++IQA +KG + RK +R + A E ++G DI DL+DPEVEKAAT+IQAGY+G++TR+ +K ++ E VP E++ DIDL DPEVE+AA KIQ+ +KG+RAR+ SA S+K E +EIDID+ DP+ E+AA KIQA ++G+++RR M+ V+ P E+ IDIDL DPEV +AA KIQ+ +KG++ RK K + +Q+ ++IDL+DP++A+AA KIQ+ ++G ++RK +++ S NI S ++ VDIDL+DP+VEKAA KIQ+ +KG+K+RK ++ P +E + L + ++AA KIQA ++G+++RK + P S + + P P P E IDIDL+DPEVE+AA KIQA +KG+++RK + K +QP + +DIDLTDP+VE+AA KIQA +KG+R R+ + P+EA I + DPE QAA KIQAG+RG +TR+ E+ ESEGD ++ + P E E A+ + E P++ID+ P++ +AATKIQAGF+G + RK ++G
Sbjct: 79 VDSGDPQQQDAALKIQTEYRRHSAKKEVDDMREE-----------------DAATTIQAGVRGHQDRQKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSP---SLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRK--------------EVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMKG 966 HSP 4 Score: 458.373 bits (1178), Expect = 2.336e-132 Identity = 338/919 (36.78%), Postives = 499/919 (54.30%), Query Frame = 0
Query: 273 IDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRR---EMRGPALALPSNSMDAD--DSSQELAATKIQAGYRGMRVRKGLKATQ-------AENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPN----DSEP--MDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANE------------VIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEA-IDI------------------DLEDPDMERAATKIQAGYKGMRARKELRQNKEE------LKDT--------EAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAA---------------------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAE-----------------QGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ--------------GMKPVEAEPV--PEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKA-----------------EDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVE---------------PAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK---------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLR---ALPEEFPEES-------------PETGLTEDQAATKIQAGYRGMRARKEIVPLNL------------SEMTRDGDPF-------PEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMR 986
+D DP+ Q+AA KIQ +R A+K+ +DD E AAT IQAG +G + R+ E++ L S +D + D E AA KIQAG++G + RK +K ++ E +E +DIDL+DPEV +AATKIQAG++G + RKEL+ ++ P ++EP +DIDL DPEV +AATKIQAG+KG + RK++ N ++P ++ DID DP++ +AATKIQA +KG + R+ +R++Q D DI DL DP++E+AATKIQAGYKG++ R+EL+ +E ++D+ E IDIDL DPEVE+AA KIQ+ +KG+R+RKS AA E Q+EIDIDL DPEV AA +IQA +KG+++R+ +K++ + +IDIDLNDPEV KAA +IQ+ ++G+++R+ + P + PV P +D DIDL DPEV +AA KIQ+ +KG+++R+ ++T S ++ E+E+DID+ DP+ E+AA KIQ+ ++G+++R+ ++ P ++ IDIDL DPEV++AA KIQA +KG++ RK + + ++IDL+DP++ +AA KIQA ++G ++RK + + V+ K P S ++ E + +DIDL+DPDVEKAA KIQA +KG++ RKT A P+E +++ PET QAA KIQAG+RG + RKE V NL SE T+ + E+ + E +DIDL+DPEV +AATKIQAG+KG + RK M+
Sbjct: 79 VDSGDPQQQDAALKIQTEYRRHSAKKE---------------------VDDMREEDAATTIQAGVRGHQDRQKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEM---NGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRK--FGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETA----QAALKIQAGFRGYKTRKE-VKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 965 HSP 5 Score: 434.491 bits (1116), Expect = 5.931e-124 Identity = 328/937 (35.01%), Postives = 479/937 (51.12%), Query Frame = 0
Query: 482 IDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEAIDIDLEDPDMERAATKIQAGYKGMRARK---ELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREK-------AAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMRE-------KAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ---GMKPVE-----AEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDID--------------------MEDPDEERAATKIQAGYRGMRTRREMQGRNEI-------------VEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--------------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTR-----KTLRALP---EEFPEESPE-TGLTEDQ------------AATKIQAGYRGMRARK-------EIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE------------------EQPDAMDIDLTDPEVERAATKIQAGYKGMRTRR-------------------ELKIPYPEEA-IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG---EPINIDMTAPDMEQAATKIQAGFRGMQARKG----------IQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLA-----------ARRPDIET-------------VESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDL 1231
+D DP+ + AA KIQ Y+ A++ + +EE AAT IQAG +G + R+ E+++ + LK E +DI+LEDPEVE AA KIQAG+KG ++RK ++E E ++E+DIDLEDPEV AA++IQAG++G +TRK ++ + AE IDIDLNDPEV KAAT+IQAG++G +TR+ G +P+ + + DT DID DP+V +AATKIQA +KG + R+ +R + S D D + DP+ E+AATKIQAGY+G++TRRE++ E V E+ IDIDLTDPEV++AA KIQ+ +KG+R RK K + + ++IDL+DP++ +AA KIQA ++G ++R+ + +N N+ ++ ++ +DIDL+DP+V KAA KIQ+ +KG+K+R KT ++ P P SP T ED+ AA KIQ+ ++G+++RK + P N+ E +DIDL+DPEVE+AA KIQ+ +KG+++RK +K+ + +DIDL DPEVE+AA KIQA +KG+++R+ + IP EE I + DPE+E+AA KIQA ++G+++R+ K P + P L S+ VDA E I+ID+T PD+E+AA KIQA F+G++ARK + + E+ DIDLNDPE AA KIQAG+ G +TR+++ +P ET E +E + D E AATKIQAGFKG K R+D+
Sbjct: 79 VDSGDPQQQDAALKIQTEYRRHSAKKEVDDMREE-----------------DAATTIQAGVRGHQDRQKVDEIKEGEARLKSAE-VDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPV---PVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKP---------VAP---------------------LKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDM 964 HSP 6 Score: 352.829 bits (904), Expect = 3.421e-96 Identity = 282/841 (33.53%), Postives = 434/841 (51.61%), Query Frame = 0
Query: 566 IDLEDPEVERAATKIQAGYKGMRSRKSMREKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKS---MREKAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVE----AEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSA-SIKAEDE------IDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAE--------DPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQ-ALEID-------------------------LDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKT---------LRALPEEFPEESPETGLTEDQ--------------AATKIQAGYRGMRAR----KEIVPLN----LSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQP-------------------DAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEA----------------------IHMADPEMEQAATKIQAGYRGMRTRR-GMKPIPDVEV------VVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG------------------------EPINIDMTAPDMEQAATKIQAGFRGMQARK-----------------GIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRR-----DLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQ 1233
+D DP+ + AA KIQ Y+ ++K + D+ ED AA+ IQAG +G + R+ ++E A + ++DI+L DPEVE AA +IQAG++G + R+ +K + P + + DIDLEDPEV +AATKIQAG++G + R+ L++S + K+E E IDID+ DP+ +AATKIQAG++G +TR+EM G+ + + D DID DP+V +AATKIQA +KG + RK++R+NQ A E D L DP++ +AATKIQAGY+G + R++ S +E S + S++ N E++I+DIDL+DP+VEKAA KIQ+ +KG++ RK+ + + P P + TE Q AA KIQA ++G+++R K+ P N + +T D P E E IDIDL+DPEV +AA KIQ+ +KG+++RK + K + D +DIDL DPEV +AA KIQ+ +KG+++R+ +K ++ I + DPE+E+AA KIQ+ ++G+++R+ G K + V +TP + E D+ DP+ E ++ + +G E I+ID+ P++E+AA KIQA F+G+++RK + + ++ E+ DIDL DP+VE AA KIQA + G+R R+ + A + + +T EE+ I D ET AA KIQAGF+G K R+++ +
Sbjct: 79 VDSGDPQQQDAALKIQTEYRRHSAKKEVD----------DMREED-----AATTIQAGVRGHQDRQKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSME--NVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVE---EEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEE-IDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDL--NDPETAQAALKIQAGFRGYKTRKEVKE 895 HSP 7 Score: 149.443 bits (376), Expect = 3.328e-32 Identity = 120/364 (32.97%), Postives = 173/364 (47.53%), Query Frame = 0
Query: 915 AATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMRE----KAEEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEA-------------IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAE-----------DAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIG--------------SYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
AA KIQ YR A+KE+ D E AAT IQAG +G + R+ + E +A + +DI+L DPEVE AA KIQAG+KG + R+E+K +EA I + DPE+ +AATKIQAG+RG +TR+ +K E P+SE+E +A E I+ID+ P++ +AATKIQAGF+G + RK + G+ ++ D DID NDP+V AA+KIQA + G + R+++ + ET + ++ D E AATKIQAG+KG+K RR+L +++
Sbjct: 89 AALKIQTEYRRHSAKKEV----------------------------DDMREEDAATTIQAGVRGHQDRQKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVK--ESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELK---------SSEDAPKSETE--------------------------NAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQE 387
BLAST of sperm surface protein sp17 vs. nr
Match: gi|1207967344|ref|XP_021374267.1| (titin-like isoform X6 [Mizuhopecten yessoensis]) HSP 1 Score: 518.464 bits (1334), Expect = 2.177e-153 Identity = 379/1028 (36.87%), Postives = 550/1028 (53.50%), Query Frame = 0
Query: 9 RLNLPTGLEESLEGLAKEVLRSQPSNILHFSAEYFENQGLPANQDIFDYIALSSDVNPNKYDNAPPLEDGTEVENLSGDDIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEA--EEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASP-----------------------IEIDL-ADPEVEKAATKIQAGFKGMRTRKQMRKAEPV-------PEELADIDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDE--------VDIDLDDPEVQEAATKIQAGFRGMKARKQ--AKPEAKDEEDSQENIPSI---DIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSMDAD----------------------DSSQELAATKIQAGYRGMRVRKGLKATQ------------AEN----EEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQH------QEEPNDSEP-------------MDIDLEDPEVERAATKIQAGYKGMRAR-----KQLKRDN-----------EEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARR----ALRQHQEEIN-------------ADEAIDIDLEDPDMERAATKIQAGYKGMRARK-ELRQNKEE--------------LKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAA------------------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEA---------------EQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ--GMKPVEAEPVP-----------EDDTFDIDLEDPEVERAATKIQAGYKGMRAR--RGLRTSASIKA-------EDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQ-------GRNEIVEPA---------------------EDPIDIDLTDPEVDRAATKIQAGYKGMRERKKLR 802
+L +P G + LEGLA+EVLR+QP NI F A+YF+ ++ D + + Y+N + +D DP AA KIQ YR A++++ R++++ + IQ V+ D + E+ + EA + AE+DI+LEDPEV AA KIQAG++G K RK ++ + E DL DP ++AA KIQAGF+G + RK+++ + +E DIDL+DPE+ +AATKIQAG+RG +TR+ +K + P+ E +DIDL+DPEV +AATKIQAGF+G K RK+ K D++ + DID +DP+V KAATKIQA +KG +VR+E+R A ++ D D E AATKIQAGY+G++ R+ LK+ Q EN EE IDIDL DPEVE+AA KIQ+ +KG+RARK + EP+++ P +DIDL DPEVE+AA KIQA +KG+++R K +N ++ P E IDIDL DPE+ +AA KIQ+ +KG+++R+ Q IN ++ IDIDL DP++ +AA KIQ+ +KG+++RK + +Q+ + + E +DIDL DPEVE+AA KIQ+ +KG++SRK +K++ ++EIDIDL DPEV AA +IQA +KG+++RK +K++ + + +IDIDLNDPEVEKAA +IQA ++G+++R+ KPV P E + DIDL DP+VE+AA KIQA +KG+RAR + + +A +A E+EIDID+ DP+ +AA KIQAG+RG +TR+E++ + E +P+ E+P+DIDL DPEV +AATKIQAG+KG + RK ++
Sbjct: 9 KLRVPKGFQNILEGLAREVLRNQPENIYEFGAKYFDQLLRVRDETGHDPAVHGARMEDRFYNN----------DAFRSPGVDSGDPQQQDAALKIQTEYRRHSAKKEVDDMREEDA--ATTIQAGVRGHQDRQKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKSKSGEATEEADQGETTVTEKESEEAEADLELDPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1024 HSP 2 Score: 486.108 bits (1250), Expect = 8.676e-142 Identity = 358/974 (36.76%), Postives = 529/974 (54.31%), Query Frame = 0
Query: 233 IDLDDPEMNQAATKIQAGYRGMQTRRGMKVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEEDSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMR---------------GPALALPSNSMDAD-----DSSQELAATKIQAGYRGMRVRKGLKATQ-------AENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPN----DSEP--MDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANE------------VIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEA-IDI------------------DLEDPDMERAATKIQAGYKGMRARKELRQNKEE------LKDT--------EAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAA---------------------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAE-----------------QGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ--------------GMKPVEAEPV--PEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKA-----------------EDEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVE---------------PAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK---------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLR---ALPEEFPEES-------------PETGLTEDQAATKIQAGYRGMRARKEIVPLNL------------SEMTRDGDPF-------PEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMR 986
+D DP+ AA KIQ YR ++ ++DD ++AAT IQAG RG + R++ E K+ E ++ +DI+L+DPEVE AA KIQAG+KG + R+E++ G S +A+ D + + AA KIQAG+RG + RK +KA + E +E +DIDL+DPEV +AATKIQAG++G + RKEL+ ++ P ++EP +DIDL DPEV +AATKIQAG+KG + RK++ N ++P ++ DID DP++ +AATKIQA +KG + R+ +R++Q D DI DL DP++E+AATKIQAGYKG++ R+EL+ +E ++D+ E IDIDL DPEVE+AA KIQ+ +KG+R+RKS AA E Q+EIDIDL DPEV AA +IQA +KG+++R+ +K++ + +IDIDLNDPEV KAA +IQ+ ++G+++R+ + P + PV P +D DIDL DPEV +AA KIQ+ +KG+++R+ ++T S ++ E+E+DID+ DP+ E+AA KIQ+ ++G+++R+ ++ P ++ IDIDL DPEV++AA KIQA +KG++ RK + + ++IDL+DP++ +AA KIQA ++G ++RK + + V+ K P S ++ E + +DIDL+DPDVEKAA KIQA +KG++ RKT A P+E +++ PET QAA KIQAG+RG + RKE V NL SE T+ + E+ + E +DIDL+DPEV +AATKIQAG+KG + RK M+
Sbjct: 79 VDSGDPQQQDAALKIQTEYRRHSAKK---------------EVDDMREEDAATTIQAGVRGHQDRQKVD-EIKEGEARLKSA-EVDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKSKSGEATEEADQGETTVTEKESEEAEADLELDPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEM---NGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRK--FGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETA----QAALKIQAGFRGYKTRKE-VKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1024 HSP 3 Score: 483.411 bits (1243), Expect = 6.933e-141 Identity = 368/988 (37.25%), Postives = 537/988 (54.35%), Query Frame = 0
Query: 352 ALPSNSMDADDSSQELAATKIQAGYRGMRVRKGLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARK---ELRQHQEEPNDSEPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRD------------------NEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQE-----EINADEAIDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEELK------DTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSM-----------REKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMR--EKAAEAEQGDIDI------------------DLNDPEVEKAATRIQAGYRGMRTRQGMKPVE------------AEPVP-EDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSA------------------SIKAE--DEIDIDMEDPDEERAATKIQAGYRGMRTRR-------------EMQGRNEIVE--PAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--KLRENQA----------------------LEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRK--TLRALPEEF---------------PEESPETGLTE---DQAATKIQAGYRGMRARK----EIVPLNLSEMTRDGDPFPEEGEP--EAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE---EQPDA-------------MDIDLTDPEVERAATKIQAGYKGMRTRR--ELKIPYPEEA------------IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMD-PQNELVEGELDHAASAAEVDAYGE-PINIDMTAPDMEQAATKIQAGFRGMQARKGIQG 1146
A S +D+ D Q+ AA KIQ YR +K ++DD E AAT IQAG +G + R+ E+++ + +E +DI+LEDPEVE AA KIQAG+KG +ARK++K E+E D++L DP ++AA KIQAG++G +AR+ ++ +E E +DIDLEDP++ +AATKIQAG++G + RKEL+ +++ K + E IDIDL DPEV +AATKIQAG+KG ++RK M R + D DID DP+V AA++IQA +KG + RK +R + A E ++G DI DL+DPEVEKAAT+IQAGY+G++TR+ +K ++ E VP E++ DIDL DPEVE+AA KIQ+ +KG+RAR+ SA S+K E +EIDID+ DP+ E+AA KIQA ++G+++RR M+ V+ P E+ IDIDL DPEV +AA KIQ+ +KG++ RK K + +Q+ ++IDL+DP++A+AA KIQ+ ++G ++RK +++ S NI S ++ VDIDL+DP+VEKAA KIQ+ +KG+K+RK ++ P +E + L + ++AA KIQA ++G+++RK + P S + + P P P E IDIDL+DPEVE+AA KIQA +KG+++RK + K +QP + +DIDLTDP+VE+AA KIQA +KG+R R+ + P+EA I + DPE QAA KIQAG+RG +TR+ E+ ESEGD ++ + P E E A+ + E P++ID+ P++ +AATKIQAGF+G + RK ++G
Sbjct: 73 AFRSPGVDSGDPQQQDAALKIQTEYRRHSAKK---------------EVDDMREEDAATTIQAGVRGHQDRQKVDEIKEGEARLKSAE-VDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKSKSGEATEEADQGETTVTEKESEEAEADLEL-DPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSP---SLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRK--------------EVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMKG 1025 HSP 4 Score: 452.981 bits (1164), Expect = 5.107e-130 Identity = 348/999 (34.83%), Postives = 509/999 (50.95%), Query Frame = 0
Query: 440 IDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANEVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEA------IDIDLEDPDMERAATKIQAGYKGMRARKELRQNKEEL----------------KDTEAIDIDLE-DPEVERAATKIQAGYKGMRSRKSMREK-------AAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMRE-------KAAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ---GMKPVE-----AEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDID--------------------MEDPDEERAATKIQAGYRGMRTRREMQG-------------RNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--------------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTR-----KTLRALP---EEFPEESPE-TGLTEDQ------------AATKIQAGYRGMRARK-------EIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE------------------EQPDAMDIDLTDPEVERAATKIQAGYKGMRTRR-------------------ELKIPYPEEA-IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG---EPINIDMTAPDMEQAATKIQAGFRGMQARKG----------IQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLA-----------ARRPDIET-------------VESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDL 1231
+D DP+ + AA KIQ Y+ A+K++ EE+ AAT IQAG +G + R Q +EI EA +DI+LEDP++E AA KIQAG+KG +ARKE++++K + K++E + DLE DP + AA KIQAG++G ++RK ++ K E ++E+DIDLEDPEV AA++IQAG++G +TRK ++ + AE IDIDLNDPEV KAAT+IQAG++G +TR+ G +P+ + + DT DID DP+V +AATKIQA +KG + R+ +R + S D D + DP+ E+AATKIQAGY+G++TRRE++ E V E+ IDIDLTDPEV++AA KIQ+ +KG+R RK K + + ++IDL+DP++ +AA KIQA ++G ++R+ + +N N+ ++ ++ +DIDL+DP+V KAA KIQ+ +KG+K+R KT ++ P P SP T ED+ AA KIQ+ ++G+++RK + P N+ E +DIDL+DPEVE+AA KIQ+ +KG+++RK +K+ + +DIDL DPEVE+AA KIQA +KG+++R+ + IP EE I + DPE+E+AA KIQA ++G+++R+ K P + P L S+ VDA E I+ID+T PD+E+AA KIQA F+G++ARK + + E+ DIDLNDPE AA KIQAG+ G +TR+++ +P ET E +E + D E AATKIQAGFKG K R+D+
Sbjct: 79 VDSGDPQQQDAALKIQTEYRRHSAKKEVDDMREED-----------------AATTIQAGVRGHQDR----QKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKSKSGEATEEADQGETTVTEKESEEAEADLELDPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPV---PVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKP---------VAP---------------------LKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDM 1023 HSP 5 Score: 377.481 bits (968), Expect = 3.909e-104 Identity = 303/903 (33.55%), Postives = 462/903 (51.16%), Query Frame = 0
Query: 524 IDLEDPDMERAATKIQAGYKGMRARKELRQNKEELKDTEAIDIDLEDPEVERAATKIQAGYKGMRSRKSMRE----KAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAA-------------------EAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMKPVE----AEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSA-SIKAEDE------IDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAE--------DPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQ-ALEID-------------------------LDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKT---------LRALPEEFPEESPETGLTEDQ--------------AATKIQAGYRGMRAR----KEIVPLN----LSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQP-------------------DAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEA----------------------IHMADPEMEQAATKIQAGYRGMRTRR-GMKPIPDVEV------VVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG------------------------EPINIDMTAPDMEQAATKIQAGFRGMQARK-----------------GIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRR-----DLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQ 1233
+D DP + AA KIQ Y+ A+KE+ +EE AAT IQAG +G + R+ + E +A E+DI+LEDPEV AA +IQAG+KG + RK ++E + E+E+ + D++L DP ++AA +IQAG+RG + R+ +K + P + + DIDLEDPEV +AATKIQAG++G + R+ L++S + K+E E IDID+ DP+ +AATKIQAG++G +TR+EM G+ + + D DID DP+V +AATKIQA +KG + RK++R+NQ A E D L DP++ +AATKIQAGY+G + R++ S +E S + S++ N E++I+DIDL+DP+VEKAA KIQ+ +KG++ RK+ + + P P + TE Q AA KIQA ++G+++R K+ P N + +T D P E E IDIDL+DPEV +AA KIQ+ +KG+++RK + K + D +DIDL DPEV +AA KIQ+ +KG+++R+ +K ++ I + DPE+E+AA KIQ+ ++G+++R+ G K + V +TP + E D+ DP+ E ++ + +G E I+ID+ P++E+AA KIQA F+G+++RK + + ++ E+ DIDL DP+VE AA KIQA + G+R R+ + A + + +T EE+ I D ET AA KIQAGF+G K R+++ +
Sbjct: 79 VDSGDPQQQDAALKIQTEYRRHSAKKEVDDMREE-----------------DAATTIQAGVRGHQDRQKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKSKSGEATEEADQGETTVTEKESEEAEADLEL-DPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKSSEDAPKSETENAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSME--NVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVE---EEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEE-IDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDL--NDPETAQAALKIQAGFRGYKTRKEVKE 954 HSP 6 Score: 172.555 bits (436), Expect = 2.328e-39 Identity = 135/425 (31.76%), Postives = 206/425 (48.47%), Query Frame = 0
Query: 867 IDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMRE------KAEEQPDAMDIDLT-------------DPEVERAATKIQAGYKGMRTRRELKIPYPEEA-----------IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAE-----------DAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIG--------------SYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
+D DP + AA KIQ Y+ +K + + E+ AAT IQAG RG + R+++ + ++G+ + E +DI+L DPEVE AA KIQAG+KG + RK ++E +A E+ D + +T DP + AA KIQAG++G + R+E+K + I + DPE+ +AATKIQAG+RG +TR+ +K E P+SE+E +A E I+ID+ P++ +AATKIQAGF+G + RK + G+ ++ D DID NDP+V AA+KIQA + G + R+++ + ET + ++ D E AATKIQAG+KG+K RR+L +++
Sbjct: 79 VDSGDPQQQDAALKIQTEYRRHSAKKEV-------------DDMREEDAATTIQAGVRGHQDRQKV------DEIKEGEARLKSAE---VDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKSKSGEATEEADQGETTVTEKESEEAEADLELDPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELK---------SSEDAPKSETE--------------------------NAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQE 446
BLAST of sperm surface protein sp17 vs. nr
Match: gi|1207967346|ref|XP_021374268.1| (enolase-phosphatase E1-like isoform X7 [Mizuhopecten yessoensis]) HSP 1 Score: 389.808 bits (1000), Expect = 5.889e-109 Identity = 282/734 (38.42%), Postives = 389/734 (53.00%), Query Frame = 0
Query: 151 AEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPI----------------EIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEE---LADI------------------DLDDPEMNQAATKIQAGYRGMQTRRGMK-----------VHDKQ---PEDE--VDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAK----DEEDSQENIPS-----------IDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSM---------------------DADDSSQELAATKIQAGYRGMRVRKGLKATQAEN-------------------EEGIDIDLDDPEVERAATKIQAGYKGMRARK-ELRQHQEEPNDS--------------EPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPAN------------------EVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQE--------------EINADEAIDIDLEDPDMERAATKIQAGYKGMRARK--------ELRQ----NKEELKDTEA--IDIDLEDPEVERAATKIQAGYKGMRSRKSMR---------EKAAEHQ-DEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMR------------EKAAE------------------AEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMK 675
AE ++DIDL DPEVA AATKIQAG++G KTRK + P+ +ID DP+V KAATKIQA FKG + RK++RK + PE DI DL DPE+ +AATKIQAGY+G++TRR +K V D P +E +DIDL DPEV++AA KIQ+ F+G++ARK A + E S P IDIDL+DPEVEKAA KIQA +KG++ RR G + P+N++ D +D AA KIQ+ ++G++ RK K +++ E+ IDIDL+DPEV +AA KIQ+ +KG+++RK + +Q + P + E +DIDL DPEVE+AA KIQ+ +KG+++RK + + P N E IDIDL DPE+E+AA KIQA +KG+++R+ + I ++E IDIDL DP++E+AA KIQA +KG+++RK L+Q N + K +E IDIDL DP+VE+AA KIQA +KG+R+RK+ EK E Q +EIDIDL DPE AA +IQAG++G +TRK ++ EK +E ++ +DIDLNDPEV KAAT+IQAG++G + R+ MK
Sbjct: 481 AEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRR--FGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKS--SPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1210 HSP 2 Score: 378.252 bits (970), Expect = 4.378e-105 Identity = 272/738 (36.86%), Postives = 400/738 (54.20%), Query Frame = 0
Query: 436 EPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANE------------VIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEA-IDI------------------DLEDPDMERAATKIQAGYKGMRARKELRQNKEE------LKDT--------EAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAA---------------------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAE-----------------QGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ--------------GMKPVEAEPV--PEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKA-----------------EDEIDIDMEDPDEERAATKIQAGYRGMRTRR---------------EMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK---------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLR---ALPEEFPEES-------------PETGLTEDQAATKIQAGYRGMRARKEIVPLNL------------SEMTRDGDPF-------PEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMR 986
E +DIDL DPEV +AATKIQAG+KG + RK++ N ++P ++ DID DP++ +AATKIQA +KG + R+ +R++Q D DI DL DP++E+AATKIQAGYKG++ R+EL+ +E ++D+ E IDIDL DPEVE+AA KIQ+ +KG+R+RKS AA E Q+EIDIDL DPEV AA +IQA +KG+++R+ +K++ + +IDIDLNDPEV KAA +IQ+ ++G+++R+ + P + PV P +D DIDL DPEV +AA KIQ+ +KG+++R+ ++T S ++ E+E+DID+ DP+ E+AA KIQ+ ++G+++R+ + P ++ IDIDL DPEV++AA KIQA +KG++ RK + + ++IDL+DP++ +AA KIQA ++G ++RK + + V+ K P S ++ E + +DIDL+DPDVEKAA KIQA +KG++ RKT A P+E +++ PET QAA KIQAG+RG + RKE V NL SE T+ + E+ + E +DIDL+DPEV +AATKIQAG+KG + RK M+
Sbjct: 484 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEM---NGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRK--FGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETA----QAALKIQAGFRGYKTRKE-VKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1210 HSP 3 Score: 371.703 bits (953), Expect = 8.615e-103 Identity = 280/746 (37.53%), Postives = 405/746 (54.29%), Query Frame = 0
Query: 562 EAIDIDLEDPEVERAATKIQAGYKGMRSRKSM-----------REKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMR--EKAAEAEQGDIDI------------------DLNDPEVEKAATRIQAGYRGMRTRQGMKPVEA------------EPVP-EDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSA------------------SIKAE--DEIDIDMEDPDEERAATKIQAGYRGMRTRR-------------EMQGRNEIVE--PAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--KLRENQA----------------------LEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRK--TLRALPEEF---------------PEESPETGLTE---DQAATKIQAGYRGMRARK----EIVPLNLSEMTRDGDPFPEEGEP--EAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE---EQP-------------DAMDIDLTDPEVERAATKIQAGYKGMRTRR------------ELKIPYPEEAIH--MADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMD-PQNELVEGELDHAASAAEVDAYGE-PINIDMTAPDMEQAATKIQAGFRGMQARKGIQG 1146
E IDIDL DPEV +AATKIQAG+KG ++RK M R + D DID DP+V AA++IQA +KG + RK +R + A E ++G DI DL+DPEVEKAAT+IQAGY+G++TR+ +K ++ E VP E++ DIDL DPEVE+AA KIQ+ +KG+RAR+ SA S+K E +EIDID+ DP+ E+AA KIQA ++G+++RR M+ V+ P E+ IDIDL DPEV +AA KIQ+ +KG++ RK K + +Q+ ++IDL+DP++A+AA KIQ+ ++G ++RK +++ S NI S ++ VDIDL+DP+VEKAA KIQ+ +KG+K+RK ++ P +E + L + ++AA KIQA ++G+++RK + P ++++ P P P E IDIDL+DPEVE+AA KIQA +KG+++RK + K +QP + +DIDLTDP+VE+AA KIQA +KG+R R+ E K EE I + DPE QAA KIQAG+RG +TR+ E+ ESEGD ++ + P E E A+ + E P++ID+ P++ +AATKIQAGF+G + RK ++G
Sbjct: 484 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPT---PPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRK--------------EVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMKG 1211 HSP 4 Score: 342.813 bits (878), Expect = 3.219e-93 Identity = 261/762 (34.25%), Postives = 379/762 (49.74%), Query Frame = 0
Query: 640 AEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ---GMKPVE-----AEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDID--------------------MEDPDEERAATKIQAGYRGMRTRREMQGRNEI-------------VEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--------------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTR-----KTLRALP---EEFPEESPE-TGLTEDQ------------AATKIQAGYRGMRARK-------EIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKA------------------EEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRR-------------------ELKIPYPEEA-IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG---EPINIDMTAPDMEQAATKIQAGFRGMQARKG----------IQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLA-----------ARRPDIET-------------VESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDL 1231
AE IDIDLNDPEV KAAT+IQAG++G +TR+ G +P+ + + DT DID DP+V +AATKIQA +KG + R+ +R + S D D + DP+ E+AATKIQAGY+G++TRRE++ E V E+ IDIDLTDPEV++AA KIQ+ +KG+R RK K + + ++IDL+DP++ +AA KIQA ++G ++R+ + +N N+ ++ ++ +DIDL+DP+V KAA KIQ+ +KG+K+R KT ++ P P SP T ED+ AA KIQ+ ++G+++RK + P N+ E +DIDL+DPEVE+AA KIQ+ +KG+++RK +K+ + +DIDL DPEVE+AA KIQA +KG+++R+ + IP EE I + DPE+E+AA KIQA ++G+++R+ K P + P L S+ VDA E I+ID+T PD+E+AA KIQA F+G++ARK + + E+ DIDLNDPE AA KIQAG+ G +TR+++ +P ET E +E + D E AATKIQAGFKG K R+D+
Sbjct: 481 AEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPV---PVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKP---------VAP---------------------LKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDM 1209 HSP 5 Score: 270.781 bits (691), Expect = 6.683e-70 Identity = 217/666 (32.58%), Postives = 335/666 (50.30%), Query Frame = 0
Query: 727 DEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAE--------DPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQ-ALEID-------------------------LDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKT---------LRALPEEFPEESPETGLTEDQ--------------AATKIQAGYRGMRAR----KEIVPLN----LSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQP-------------------DAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEA----------------------IHMADPEMEQAATKIQAGYRGMRTRR-GMKPIPDVEV------VVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG------------------------EPINIDMTAPDMEQAATKIQAGFRGMQARK-----------------GIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRR-----DLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQ 1233
++IDID+ DP+ +AATKIQAG++G +TR+EM G+ + + D DID DP+V +AATKIQA +KG + RK++R+NQ A E D L DP++ +AATKIQAGY+G + R++ S +E S + S++ N E++I+DIDL+DP+VEKAA KIQ+ +KG++ RK+ + + P P + TE Q AA KIQA ++G+++R K+ P N + +T D P E E IDIDL+DPEV +AA KIQ+ +KG+++RK + K + D +DIDL DPEV +AA KIQ+ +KG+++R+ +K ++ I + DPE+E+AA KIQ+ ++G+++R+ G K + V +TP + E D+ DP+ E ++ + +G E I+ID+ P++E+AA KIQA F+G+++RK + + ++ E+ DIDL DP+VE AA KIQA + G+R R+ + A + + +T EE+ I D ET AA KIQAGF+G K R+++ +
Sbjct: 484 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSME--NVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVE---EEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEE-IDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLN--DPETAQAALKIQAGFRGYKTRKEVKE 1140 HSP 6 Score: 85.8853 bits (211), Expect = 7.624e-13 Identity = 53/153 (34.64%), Postives = 82/153 (53.59%), Query Frame = 0
Query: 1109 DAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAE-----------DAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIG--------------SYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
+A E I+ID+ P++ +AATKIQAGF+G + RK + G+ ++ D DID NDP+V AA+KIQA + G + R+++ + ET + ++ D E AATKIQAG+KG+K RR+L +++
Sbjct: 480 NAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQE 632 HSP 7 Score: 77.7962 bits (190), Expect = 2.012e-10 Identity = 53/136 (38.97%), Postives = 76/136 (55.88%), Query Frame = 0
Query: 88 DIDLNDPDVAVAATKIQAGYRGSKARRDMK---SRRDDESKQSIKIQMPVQTMDDEDEGLELD-DPEAEEA---------------------------------ELDIDLEDPEVANAATKIQAGYRGMKTRKHLR 186
DI+L DP+V AA KIQAG++G KAR+++K S+ + ++++ + + V + E+ +L+ DP A+EA E+DIDLEDPEVA AATKIQAG+RG KTRK L+
Sbjct: 147 DINLEDPEVEAAAVKIQAGFKGYKARKEVKESKSKSGEATEEADQGETTVTEKESEEAEADLELDPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELK 282
BLAST of sperm surface protein sp17 vs. nr
Match: gi|1207967342|ref|XP_021374266.1| (titin-like isoform X5 [Mizuhopecten yessoensis]) HSP 1 Score: 391.734 bits (1005), Expect = 2.344e-108 Identity = 280/734 (38.15%), Postives = 388/734 (52.86%), Query Frame = 0
Query: 151 AEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPI----------------EIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEE---LADI------------------DLDDPEMNQAATKIQAGYRGMQTRRGMK-----------VHDKQ---PEDE--VDIDLDDPEVQEAATKIQAGFRGMKARK----QAKPEAKDEEDSQENIPS-----------IDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSM---------------------DADDSSQELAATKIQAGYRGMRVRKGLKATQAEN-------------------EEGIDIDLDDPEVERAATKIQAGYKGMRARK-ELRQHQEEPNDS--------------EPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPAN------------------EVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQE--------------EINADEAIDIDLEDPDMERAATKIQAGYKGMRARK--------ELRQ----NKEELKDTEA--IDIDLEDPEVERAATKIQAGYKGMRSRKSMR---------EKAAEHQ-DEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREK------------------------------AAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMK 675
AE ++DIDL DPEVA AATKIQAG++G KTRK + P+ +ID DP+V KAATKIQA FKG + RK++RK + PE DI DL DPE+ +AATKIQAGY+G++TRR +K V D P +E +DIDL DPEV++AA KIQ+ F+G++ARK A + + E S P IDIDL+DPEVEKAA KIQA +KG++ RR G + P+N++ D +D AA KIQ+ ++G++ RK K +++ E+ IDIDL+DPEV +AA KIQ+ +KG+++RK + +Q + P + E +DIDL DPEVE+AA KIQ+ +KG+++RK + + P N E IDIDL DPE+E+AA KIQA +KG+++R+ + I ++E IDIDL DP++E+AA KIQA +KG+++RK L+Q N + K +E IDIDL DP+VE+AA KIQA +KG+R+RK+ EK E Q +EIDIDL DPE AA +IQAG++G +TRK ++E + ++ +DIDLNDPEV KAAT+IQAG++G + R+ MK
Sbjct: 422 AEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRR--FGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKS--SPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1151 HSP 2 Score: 343.969 bits (881), Expect = 1.063e-92 Identity = 261/762 (34.25%), Postives = 379/762 (49.74%), Query Frame = 0
Query: 640 AEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ---GMKPVE-----AEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDID--------------------MEDPDEERAATKIQAGYRGMRTRREMQG-------------RNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--------------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTR-----KTLRALP---EEFPEESPE-TGLTEDQ------------AATKIQAGYRGMRARK-------EIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE------------------EQPDAMDIDLTDPEVERAATKIQAGYKGMRTRR-------------------ELKIPYPEEA-IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG---EPINIDMTAPDMEQAATKIQAGFRGMQARKG----------IQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLA-----------ARRPDIET-------------VESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDL 1231
AE IDIDLNDPEV KAAT+IQAG++G +TR+ G +P+ + + DT DID DP+V +AATKIQA +KG + R+ +R + S D D + DP+ E+AATKIQAGY+G++TRRE++ E V E+ IDIDLTDPEV++AA KIQ+ +KG+R RK K + + ++IDL+DP++ +AA KIQA ++G ++R+ + +N N+ ++ ++ +DIDL+DP+V KAA KIQ+ +KG+K+R KT ++ P P SP T ED+ AA KIQ+ ++G+++RK + P N+ E +DIDL+DPEVE+AA KIQ+ +KG+++RK +K+ + +DIDL DPEVE+AA KIQA +KG+++R+ + IP EE I + DPE+E+AA KIQA ++G+++R+ K P + P L S+ VDA E I+ID+T PD+E+AA KIQA F+G++ARK + + E+ DIDLNDPE AA KIQAG+ G +TR+++ +P ET E +E + D E AATKIQAGFKG K R+D+
Sbjct: 422 AEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPV---PVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKP---------VAP---------------------LKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDM 1150 HSP 3 Score: 272.322 bits (695), Expect = 4.986e-70 Identity = 217/666 (32.58%), Postives = 335/666 (50.30%), Query Frame = 0
Query: 727 DEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAE--------DPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQ-ALEID-------------------------LDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKT---------LRALPEEFPEESPETGLTEDQ--------------AATKIQAGYRGMRAR----KEIVPLN----LSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQP-------------------DAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEA----------------------IHMADPEMEQAATKIQAGYRGMRTRR-GMKPIPDVEV------VVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG------------------------EPINIDMTAPDMEQAATKIQAGFRGMQARK-----------------GIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRR-----DLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQ 1233
++IDID+ DP+ +AATKIQAG++G +TR+EM G+ + + D DID DP+V +AATKIQA +KG + RK++R+NQ A E D L DP++ +AATKIQAGY+G + R++ S +E S + S++ N E++I+DIDL+DP+VEKAA KIQ+ +KG++ RK+ + + P P + TE Q AA KIQA ++G+++R K+ P N + +T D P E E IDIDL+DPEV +AA KIQ+ +KG+++RK + K + D +DIDL DPEV +AA KIQ+ +KG+++R+ +K ++ I + DPE+E+AA KIQ+ ++G+++R+ G K + V +TP + E D+ DP+ E ++ + +G E I+ID+ P++E+AA KIQA F+G+++RK + + ++ E+ DIDL DP+VE AA KIQA + G+R R+ + A + + +T EE+ I D ET AA KIQAGF+G K R+++ +
Sbjct: 425 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSME--NVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVE---EEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEE-IDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLN--DPETAQAALKIQAGFRGYKTRKEVKE 1081 HSP 4 Score: 127.872 bits (320), Expect = 1.220e-25 Identity = 84/227 (37.00%), Postives = 126/227 (55.51%), Query Frame = 0
Query: 9 RLNLPTGLEESLEGLAKEVLRSQPSNILHFSAEYFENQGLPANQDIFDYIALSSDVNPNKYDNAPPLEDGTEVENLSGDDIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEA--EEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPA------------SPIEIDLADPEVEKAATKIQAGFKGMRTRKQMR 221
+L +P G + LEGLA+EVLR+QP NI F A+YF+ ++ D + + Y+N + +D DP AA KIQ YR A++++ R++++ + IQ V+ D + E+ + EA + AE+DI+LEDPEV AA KIQAG++G K RK ++ + ++IDL DPEV KAATKIQAGF+G +TRK+++
Sbjct: 9 KLRVPKGFQNILEGLAREVLRNQPENIYEFGAKYFDQLLRVRDETGHDPAVHGARMEDRFYNN----------DAFRSPGVDSGDPQQQDAALKIQTEYRRHSAKKEVDDMREEDA--ATTIQAGVRGHQDRQKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELK 223 HSP 5 Score: 107.071 bits (266), Expect = 2.745e-19 Identity = 69/162 (42.59%), Postives = 98/162 (60.49%), Query Frame = 0
Query: 193 IDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEELADIDLDDPEMNQAATKIQAGYRGMQTRRGM----KVHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKARKQAKPEAKDEE--DSQENIPSIDIDLDDPEVEKAATKIQAGYKGMRVRREMRG 348
+D DP+ + AA KIQ ++ +K+ +DD AAT IQAG RG Q R+ + + + EVDI+L+DPEV+ AA KIQAGF+G KARK+ K E+K+ + +E +DIDL+DPEV KAATKIQAG++G + R+E++G
Sbjct: 79 VDSGDPQQQDAALKIQTEYRRHSAKKE---------------VDDMREEDAATTIQAGVRGHQDRQKVDEIKEGEARLKSAEVDINLEDPEVEAAAVKIQAGFKGYKARKEVK-ESKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELKG 224 HSP 6 Score: 85.8853 bits (211), Expect = 8.600e-13 Identity = 53/153 (34.64%), Postives = 82/153 (53.59%), Query Frame = 0
Query: 1109 DAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAE-----------DAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIG--------------SYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
+A E I+ID+ P++ +AATKIQAGF+G + RK + G+ ++ D DID NDP+V AA+KIQA + G + R+++ + ET + ++ D E AATKIQAG+KG+K RR+L +++
Sbjct: 421 NAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQE 573
BLAST of sperm surface protein sp17 vs. nr
Match: gi|1207967336|ref|XP_021374262.1| (titin-like isoform X2 [Mizuhopecten yessoensis]) HSP 1 Score: 391.349 bits (1004), Expect = 4.530e-108 Identity = 280/734 (38.15%), Postives = 388/734 (52.86%), Query Frame = 0
Query: 151 AEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPI----------------EIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEE---LADI------------------DLDDPEMNQAATKIQAGYRGMQTRRGMK-----------VHDKQ---PEDE--VDIDLDDPEVQEAATKIQAGFRGMKARK----QAKPEAKDEEDSQENIPS-----------IDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSM---------------------DADDSSQELAATKIQAGYRGMRVRKGLKATQAEN-------------------EEGIDIDLDDPEVERAATKIQAGYKGMRARK-ELRQHQEEPNDS--------------EPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPAN------------------EVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQE--------------EINADEAIDIDLEDPDMERAATKIQAGYKGMRARK--------ELRQ----NKEELKDTEA--IDIDLEDPEVERAATKIQAGYKGMRSRKSMR---------EKAAEHQ-DEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREK------------------------------AAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMK 675
AE ++DIDL DPEVA AATKIQAG++G KTRK + P+ +ID DP+V KAATKIQA FKG + RK++RK + PE DI DL DPE+ +AATKIQAGY+G++TRR +K V D P +E +DIDL DPEV++AA KIQ+ F+G++ARK A + + E S P IDIDL+DPEVEKAA KIQA +KG++ RR G + P+N++ D +D AA KIQ+ ++G++ RK K +++ E+ IDIDL+DPEV +AA KIQ+ +KG+++RK + +Q + P + E +DIDL DPEVE+AA KIQ+ +KG+++RK + + P N E IDIDL DPE+E+AA KIQA +KG+++R+ + I ++E IDIDL DP++E+AA KIQA +KG+++RK L+Q N + K +E IDIDL DP+VE+AA KIQA +KG+R+RK+ EK E Q +EIDIDL DPE AA +IQAG++G +TRK ++E + ++ +DIDLNDPEV KAAT+IQAG++G + R+ MK
Sbjct: 466 AEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRR--FGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKS--SPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1195 HSP 2 Score: 379.793 bits (974), Expect = 3.307e-104 Identity = 272/738 (36.86%), Postives = 400/738 (54.20%), Query Frame = 0
Query: 436 EPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANE------------VIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEA-IDI------------------DLEDPDMERAATKIQAGYKGMRARKELRQNKEE------LKDT--------EAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAA---------------------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAE-----------------QGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ--------------GMKPVEAEPV--PEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKA-----------------EDEIDIDMEDPDEERAATKIQAGYRGMRTRR---------------EMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK---------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLR---ALPEEFPEES-------------PETGLTEDQAATKIQAGYRGMRARKEIVPLNL------------SEMTRDGDPF-------PEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMR 986
E +DIDL DPEV +AATKIQAG+KG + RK++ N ++P ++ DID DP++ +AATKIQA +KG + R+ +R++Q D DI DL DP++E+AATKIQAGYKG++ R+EL+ +E ++D+ E IDIDL DPEVE+AA KIQ+ +KG+R+RKS AA E Q+EIDIDL DPEV AA +IQA +KG+++R+ +K++ + +IDIDLNDPEV KAA +IQ+ ++G+++R+ + P + PV P +D DIDL DPEV +AA KIQ+ +KG+++R+ ++T S ++ E+E+DID+ DP+ E+AA KIQ+ ++G+++R+ + P ++ IDIDL DPEV++AA KIQA +KG++ RK + + ++IDL+DP++ +AA KIQA ++G ++RK + + V+ K P S ++ E + +DIDL+DPDVEKAA KIQA +KG++ RKT A P+E +++ PET QAA KIQAG+RG + RKE V NL SE T+ + E+ + E +DIDL+DPEV +AATKIQAG+KG + RK M+
Sbjct: 469 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEM---NGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRK--FGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETA----QAALKIQAGFRGYKTRKE-VKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1195 HSP 3 Score: 372.859 bits (956), Expect = 6.512e-102 Identity = 281/746 (37.67%), Postives = 404/746 (54.16%), Query Frame = 0
Query: 562 EAIDIDLEDPEVERAATKIQAGYKGMRSRKSM-----------REKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMR--EKAAEAEQGDIDI------------------DLNDPEVEKAATRIQAGYRGMRTRQGMKPVE------------AEPVP-EDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSA------------------SIKAE--DEIDIDMEDPDEERAATKIQAGYRGMRTRR-------------EMQGRNEIVE--PAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--KLRENQA----------------------LEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRK--TLRALPEEF---------------PEESPETGLTE---DQAATKIQAGYRGMRARK----EIVPLNLSEMTRDGDPFPEEGEP--EAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE---EQP-------------DAMDIDLTDPEVERAATKIQAGYKGMRTRR------------ELKIPYPEEAIH--MADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMD-PQNELVEGELDHAASAAEVDAYGE-PINIDMTAPDMEQAATKIQAGFRGMQARKGIQG 1146
E IDIDL DPEV +AATKIQAG+KG ++RK M R + D DID DP+V AA++IQA +KG + RK +R + A E ++G DI DL+DPEVEKAAT+IQAGY+G++TR+ +K ++ E VP E++ DIDL DPEVE+AA KIQ+ +KG+RAR+ SA S+K E +EIDID+ DP+ E+AA KIQA ++G+++RR M+ V+ P E+ IDIDL DPEV +AA KIQ+ +KG++ RK K + +Q+ ++IDL+DP++A+AA KIQ+ ++G ++RK +++ S NI S ++ VDIDL+DP+VEKAA KIQ+ +KG+K+RK ++ P +E + L + ++AA KIQA ++G+++RK + P S + + P P P E IDIDL+DPEVE+AA KIQA +KG+++RK + K +QP + +DIDLTDP+VE+AA KIQA +KG+R R+ E K EE I + DPE QAA KIQAG+RG +TR+ E+ ESEGD ++ + P E E A+ + E P++ID+ P++ +AATKIQAGF+G + RK ++G
Sbjct: 469 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSP---SLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRK--------------EVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMKG 1196 HSP 4 Score: 343.969 bits (881), Expect = 1.705e-92 Identity = 261/762 (34.25%), Postives = 379/762 (49.74%), Query Frame = 0
Query: 640 AEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ---GMKPVE-----AEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDID--------------------MEDPDEERAATKIQAGYRGMRTRREMQG-------------RNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--------------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTR-----KTLRALP---EEFPEESPE-TGLTEDQ------------AATKIQAGYRGMRARK-------EIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE------------------EQPDAMDIDLTDPEVERAATKIQAGYKGMRTRR-------------------ELKIPYPEEA-IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG---EPINIDMTAPDMEQAATKIQAGFRGMQARKG----------IQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLA-----------ARRPDIET-------------VESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDL 1231
AE IDIDLNDPEV KAAT+IQAG++G +TR+ G +P+ + + DT DID DP+V +AATKIQA +KG + R+ +R + S D D + DP+ E+AATKIQAGY+G++TRRE++ E V E+ IDIDLTDPEV++AA KIQ+ +KG+R RK K + + ++IDL+DP++ +AA KIQA ++G ++R+ + +N N+ ++ ++ +DIDL+DP+V KAA KIQ+ +KG+K+R KT ++ P P SP T ED+ AA KIQ+ ++G+++RK + P N+ E +DIDL+DPEVE+AA KIQ+ +KG+++RK +K+ + +DIDL DPEVE+AA KIQA +KG+++R+ + IP EE I + DPE+E+AA KIQA ++G+++R+ K P + P L S+ VDA E I+ID+T PD+E+AA KIQA F+G++ARK + + E+ DIDLNDPE AA KIQAG+ G +TR+++ +P ET E +E + D E AATKIQAGFKG K R+D+
Sbjct: 466 AEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPV---PVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKP---------VAP---------------------LKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDM 1194 HSP 5 Score: 271.937 bits (694), Expect = 6.895e-70 Identity = 217/666 (32.58%), Postives = 335/666 (50.30%), Query Frame = 0
Query: 727 DEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAE--------DPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQ-ALEID-------------------------LDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKT---------LRALPEEFPEESPETGLTEDQ--------------AATKIQAGYRGMRAR----KEIVPLN----LSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQP-------------------DAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEA----------------------IHMADPEMEQAATKIQAGYRGMRTRR-GMKPIPDVEV------VVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG------------------------EPINIDMTAPDMEQAATKIQAGFRGMQARK-----------------GIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRR-----DLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQ 1233
++IDID+ DP+ +AATKIQAG++G +TR+EM G+ + + D DID DP+V +AATKIQA +KG + RK++R+NQ A E D L DP++ +AATKIQAGY+G + R++ S +E S + S++ N E++I+DIDL+DP+VEKAA KIQ+ +KG++ RK+ + + P P + TE Q AA KIQA ++G+++R K+ P N + +T D P E E IDIDL+DPEV +AA KIQ+ +KG+++RK + K + D +DIDL DPEV +AA KIQ+ +KG+++R+ +K ++ I + DPE+E+AA KIQ+ ++G+++R+ G K + V +TP + E D+ DP+ E ++ + +G E I+ID+ P++E+AA KIQA F+G+++RK + + ++ E+ DIDL DP+VE AA KIQA + G+R R+ + A + + +T EE+ I D ET AA KIQAGF+G K R+++ +
Sbjct: 469 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSME--NVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVE---EEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEE-IDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLN--DPETAQAALKIQAGFRGYKTRKEVKE 1125 HSP 6 Score: 266.929 bits (681), Expect = 2.470e-68 Identity = 200/540 (37.04%), Postives = 277/540 (51.30%), Query Frame = 0
Query: 59 ALSSDVNPNKYDNAPPLEDGTEVENLSGDDIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEGLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLR----------------PASP--------IEIDLADPEVEKAATKIQAGFKGMRTRKQMRK--AEPVPEEL---------------ADIDLDDPEMNQAATKIQAGYRGMQTRR-GMK-----------------VHDKQPEDEVDIDLDDPEVQEAATKIQAGFRGMKAR----KQAKPEAKDEEDSQE---NIPS---IDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALA---LPSNSMDADDSSQEL--------------AATKIQAGYRGMRVRK-----------GLKATQAENEEGIDIDLDDPEVERAATKIQAGYKGMRARKELRQHQEEPNDS-----------------------------EPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNE 472
A S+ V + PP + E DIDLNDP+V AA KIQA ++G +KSRR K S P +++ D E E+DIDL DPEVA AA KIQ+ ++G+K+RK + P+ P I+IDL DPEV KAA KIQ+ FKG+++RK K + P + DIDL+DPE+ +AA KIQ+ ++G+++R+ G K ++E+DIDL+DPEV++AA KIQA F+G+K+R K++ P ++ + NIPS IDIDL+DPEVEKAA KIQA +KG++ R+ + +A P +S D S E+ AA KIQA ++G+R RK K T+A+ EE IDIDL+DPE +AA KIQAG++G + RKE++++ E D EP+DIDL DPEV +AATKIQAG+KG + RK +K D E
Sbjct: 672 AASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKG------LKSRRFGAKKSS-----PTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEE-IDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMKGDKE 1199 HSP 7 Score: 107.071 bits (266), Expect = 2.777e-19 Identity = 78/226 (34.51%), Postives = 117/226 (51.77%), Query Frame = 0
Query: 867 IDLSDPDVEKAATKIQAGYKGMKTRKTLRALPEEFPEESPETGLTEDQAATKIQAGYRGMRARKEIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMRE------KAEEQPDAMDIDLT-------------DPEVERAATKIQAGYKGMRTRRELKIPYPEEA-----------IHMADPEMEQAATKIQAGYRGMRTRRGMK 1062
+D DP + AA KIQ Y+ +K + + EE AAT IQAG RG + R+++ + ++G+ + E +DI+L DPEVE AA KIQAG+KG + RK ++E +A E+ D + +T DP + AA KIQAG++G + R+E+K + I + DPE+ +AATKIQAG+RG +TR+ +K
Sbjct: 64 VDSGDPQQQDAALKIQTEYRRHSAKKEVDDMREE-------------DAATTIQAGVRGHQDRQKV------DEIKEGEARLKSAE---VDINLEDPEVEAAAVKIQAGFKGYKARKEVKESKSKSGEATEEADQGETTVTEKESEEAEADLELDPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELK 267 HSP 8 Score: 105.145 bits (261), Expect = 1.138e-18 Identity = 64/147 (43.54%), Postives = 91/147 (61.90%), Query Frame = 0
Query: 88 DIDLNDPDVAVAATKIQAGYRGSKARRDMKSRRDDESKQSIKIQMPVQTMDDEDEG-LELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRP------------ASPIEIDLADPEVEKAATKIQAGFKGMRTRKQMR 221
DI+L DP+V AA KIQAG++G KAR+++K + SK + ++ D+G + + E+EEAE D++L DP AA KIQAG+RG K RK ++ ++IDL DPEV KAATKIQAGF+G +TRK+++
Sbjct: 132 DINLEDPEVEAAAVKIQAGFKGYKARKEVKESK---SKSG-------EATEEADQGETTVTEKESEEAEADLEL-DPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELK 267 HSP 9 Score: 85.5001 bits (210), Expect = 9.435e-13 Identity = 53/153 (34.64%), Postives = 82/153 (53.59%), Query Frame = 0
Query: 1109 DAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAE-----------DAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIG--------------SYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
+A E I+ID+ P++ +AATKIQAGF+G + RK + G+ ++ D DID NDP+V AA+KIQA + G + R+++ + ET + ++ D E AATKIQAG+KG+K RR+L +++
Sbjct: 465 NAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQE 617
BLAST of sperm surface protein sp17 vs. nr
Match: gi|1207967334|ref|XP_021374261.1| (titin-like isoform X1 [Mizuhopecten yessoensis]) HSP 1 Score: 390.963 bits (1003), Expect = 7.986e-108 Identity = 280/734 (38.15%), Postives = 388/734 (52.86%), Query Frame = 0
Query: 151 AEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPI----------------EIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEE---LADI------------------DLDDPEMNQAATKIQAGYRGMQTRRGMK-----------VHDKQ---PEDE--VDIDLDDPEVQEAATKIQAGFRGMKARK----QAKPEAKDEEDSQENIPS-----------IDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSM---------------------DADDSSQELAATKIQAGYRGMRVRKGLKATQAEN-------------------EEGIDIDLDDPEVERAATKIQAGYKGMRARK-ELRQHQEEPNDS--------------EPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPAN------------------EVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQE--------------EINADEAIDIDLEDPDMERAATKIQAGYKGMRARK--------ELRQ----NKEELKDTEA--IDIDLEDPEVERAATKIQAGYKGMRSRKSMR---------EKAAEHQ-DEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREK------------------------------AAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMK 675
AE ++DIDL DPEVA AATKIQAG++G KTRK + P+ +ID DP+V KAATKIQA FKG + RK++RK + PE DI DL DPE+ +AATKIQAGY+G++TRR +K V D P +E +DIDL DPEV++AA KIQ+ F+G++ARK A + + E S P IDIDL+DPEVEKAA KIQA +KG++ RR G + P+N++ D +D AA KIQ+ ++G++ RK K +++ E+ IDIDL+DPEV +AA KIQ+ +KG+++RK + +Q + P + E +DIDL DPEVE+AA KIQ+ +KG+++RK + + P N E IDIDL DPE+E+AA KIQA +KG+++R+ + I ++E IDIDL DP++E+AA KIQA +KG+++RK L+Q N + K +E IDIDL DP+VE+AA KIQA +KG+R+RK+ EK E Q +EIDIDL DPE AA +IQAG++G +TRK ++E + ++ +DIDLNDPEV KAAT+IQAG++G + R+ MK
Sbjct: 481 AEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRR--FGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKS--SPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1210 HSP 2 Score: 379.407 bits (973), Expect = 5.528e-104 Identity = 272/738 (36.86%), Postives = 400/738 (54.20%), Query Frame = 0
Query: 436 EPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANE------------VIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEA-IDI------------------DLEDPDMERAATKIQAGYKGMRARKELRQNKEE------LKDT--------EAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAA---------------------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAE-----------------QGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ--------------GMKPVEAEPV--PEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKA-----------------EDEIDIDMEDPDEERAATKIQAGYRGMRTRR---------------EMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK---------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLR---ALPEEFPEES-------------PETGLTEDQAATKIQAGYRGMRARKEIVPLNL------------SEMTRDGDPF-------PEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMR 986
E +DIDL DPEV +AATKIQAG+KG + RK++ N ++P ++ DID DP++ +AATKIQA +KG + R+ +R++Q D DI DL DP++E+AATKIQAGYKG++ R+EL+ +E ++D+ E IDIDL DPEVE+AA KIQ+ +KG+R+RKS AA E Q+EIDIDL DPEV AA +IQA +KG+++R+ +K++ + +IDIDLNDPEV KAA +IQ+ ++G+++R+ + P + PV P +D DIDL DPEV +AA KIQ+ +KG+++R+ ++T S ++ E+E+DID+ DP+ E+AA KIQ+ ++G+++R+ + P ++ IDIDL DPEV++AA KIQA +KG++ RK + + ++IDL+DP++ +AA KIQA ++G ++RK + + V+ K P S ++ E + +DIDL+DPDVEKAA KIQA +KG++ RKT A P+E +++ PET QAA KIQAG+RG + RKE V NL SE T+ + E+ + E +DIDL+DPEV +AATKIQAG+KG + RK M+
Sbjct: 484 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEM---NGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRK--FGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETA----QAALKIQAGFRGYKTRKE-VKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1210 HSP 3 Score: 372.474 bits (955), Expect = 1.012e-101 Identity = 281/746 (37.67%), Postives = 404/746 (54.16%), Query Frame = 0
Query: 562 EAIDIDLEDPEVERAATKIQAGYKGMRSRKSM-----------REKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMR--EKAAEAEQGDIDI------------------DLNDPEVEKAATRIQAGYRGMRTRQGMKPVE------------AEPVP-EDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSA------------------SIKAE--DEIDIDMEDPDEERAATKIQAGYRGMRTRR-------------EMQGRNEIVE--PAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--KLRENQA----------------------LEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRK--TLRALPEEF---------------PEESPETGLTE---DQAATKIQAGYRGMRARK----EIVPLNLSEMTRDGDPFPEEGEP--EAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE---EQP-------------DAMDIDLTDPEVERAATKIQAGYKGMRTRR------------ELKIPYPEEAIH--MADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMD-PQNELVEGELDHAASAAEVDAYGE-PINIDMTAPDMEQAATKIQAGFRGMQARKGIQG 1146
E IDIDL DPEV +AATKIQAG+KG ++RK M R + D DID DP+V AA++IQA +KG + RK +R + A E ++G DI DL+DPEVEKAAT+IQAGY+G++TR+ +K ++ E VP E++ DIDL DPEVE+AA KIQ+ +KG+RAR+ SA S+K E +EIDID+ DP+ E+AA KIQA ++G+++RR M+ V+ P E+ IDIDL DPEV +AA KIQ+ +KG++ RK K + +Q+ ++IDL+DP++A+AA KIQ+ ++G ++RK +++ S NI S ++ VDIDL+DP+VEKAA KIQ+ +KG+K+RK ++ P +E + L + ++AA KIQA ++G+++RK + P S + + P P P E IDIDL+DPEVE+AA KIQA +KG+++RK + K +QP + +DIDLTDP+VE+AA KIQA +KG+R R+ E K EE I + DPE QAA KIQAG+RG +TR+ E+ ESEGD ++ + P E E A+ + E P++ID+ P++ +AATKIQAGF+G + RK ++G
Sbjct: 484 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSP---SLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRK--------------EVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMKG 1211 HSP 4 Score: 343.584 bits (880), Expect = 2.441e-92 Identity = 261/762 (34.25%), Postives = 379/762 (49.74%), Query Frame = 0
Query: 640 AEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ---GMKPVE-----AEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDID--------------------MEDPDEERAATKIQAGYRGMRTRREMQG-------------RNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--------------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTR-----KTLRALP---EEFPEESPE-TGLTEDQ------------AATKIQAGYRGMRARK-------EIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKA------------------EEQPDAMDIDLTDPEVERAATKIQAGYKGMRTRR-------------------ELKIPYPEEA-IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG---EPINIDMTAPDMEQAATKIQAGFRGMQARKG----------IQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLA-----------ARRPDIET-------------VESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDL 1231
AE IDIDLNDPEV KAAT+IQAG++G +TR+ G +P+ + + DT DID DP+V +AATKIQA +KG + R+ +R + S D D + DP+ E+AATKIQAGY+G++TRRE++ E V E+ IDIDLTDPEV++AA KIQ+ +KG+R RK K + + ++IDL+DP++ +AA KIQA ++G ++R+ + +N N+ ++ ++ +DIDL+DP+V KAA KIQ+ +KG+K+R KT ++ P P SP T ED+ AA KIQ+ ++G+++RK + P N+ E +DIDL+DPEVE+AA KIQ+ +KG+++RK +K+ + +DIDL DPEVE+AA KIQA +KG+++R+ + IP EE I + DPE+E+AA KIQA ++G+++R+ K P + P L S+ VDA E I+ID+T PD+E+AA KIQA F+G++ARK + + E+ DIDLNDPE AA KIQAG+ G +TR+++ +P ET E +E + D E AATKIQAGFKG K R+D+
Sbjct: 481 AEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPV---PVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKP---------VAP---------------------LKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDM 1209 HSP 5 Score: 271.937 bits (694), Expect = 8.425e-70 Identity = 217/666 (32.58%), Postives = 335/666 (50.30%), Query Frame = 0
Query: 727 DEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAE--------DPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQ-ALEID-------------------------LDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKT---------LRALPEEFPEESPETGLTEDQ--------------AATKIQAGYRGMRAR----KEIVPLN----LSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQP-------------------DAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEA----------------------IHMADPEMEQAATKIQAGYRGMRTRR-GMKPIPDVEV------VVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG------------------------EPINIDMTAPDMEQAATKIQAGFRGMQARK-----------------GIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRR-----DLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQ 1233
++IDID+ DP+ +AATKIQAG++G +TR+EM G+ + + D DID DP+V +AATKIQA +KG + RK++R+NQ A E D L DP++ +AATKIQAGY+G + R++ S +E S + S++ N E++I+DIDL+DP+VEKAA KIQ+ +KG++ RK+ + + P P + TE Q AA KIQA ++G+++R K+ P N + +T D P E E IDIDL+DPEV +AA KIQ+ +KG+++RK + K + D +DIDL DPEV +AA KIQ+ +KG+++R+ +K ++ I + DPE+E+AA KIQ+ ++G+++R+ G K + V +TP + E D+ DP+ E ++ + +G E I+ID+ P++E+AA KIQA F+G+++RK + + ++ E+ DIDL DP+VE AA KIQA + G+R R+ + A + + +T EE+ I D ET AA KIQAGF+G K R+++ +
Sbjct: 484 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSME--NVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVE---EEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEE-IDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLN--DPETAQAALKIQAGFRGYKTRKEVKE 1140 HSP 6 Score: 85.5001 bits (210), Expect = 9.540e-13 Identity = 53/153 (34.64%), Postives = 82/153 (53.59%), Query Frame = 0
Query: 1109 DAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAE-----------DAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIG--------------SYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
+A E I+ID+ P++ +AATKIQAGF+G + RK + G+ ++ D DID NDP+V AA+KIQA + G + R+++ + ET + ++ D E AATKIQAG+KG+K RR+L +++
Sbjct: 480 NAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQE 632 HSP 7 Score: 78.1814 bits (191), Expect = 1.986e-10 Identity = 53/136 (38.97%), Postives = 76/136 (55.88%), Query Frame = 0
Query: 88 DIDLNDPDVAVAATKIQAGYRGSKARRDMK---SRRDDESKQSIKIQMPVQTMDDEDEGLELD-DPEAEEA---------------------------------ELDIDLEDPEVANAATKIQAGYRGMKTRKHLR 186
DI+L DP+V AA KIQAG++G KAR+++K S+ + ++++ + + V + E+ +L+ DP A+EA E+DIDLEDPEVA AATKIQAG+RG KTRK L+
Sbjct: 147 DINLEDPEVEAAAVKIQAGFKGYKARKEVKESKSKSGEATEEADQGETTVTEKESEEAEADLELDPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELK 282
BLAST of sperm surface protein sp17 vs. nr
Match: gi|1207967338|ref|XP_021374264.1| (titin-like isoform X3 [Mizuhopecten yessoensis]) HSP 1 Score: 390.193 bits (1001), Expect = 1.191e-107 Identity = 280/734 (38.15%), Postives = 388/734 (52.86%), Query Frame = 0
Query: 151 AEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLRPASPI----------------EIDLADPEVEKAATKIQAGFKGMRTRKQMRKAEPVPEE---LADI------------------DLDDPEMNQAATKIQAGYRGMQTRRGMK-----------VHDKQ---PEDE--VDIDLDDPEVQEAATKIQAGFRGMKARK----QAKPEAKDEEDSQENIPS-----------IDIDLDDPEVEKAATKIQAGYKGMRVRREMRGPALALPSNSM---------------------DADDSSQELAATKIQAGYRGMRVRKGLKATQAEN-------------------EEGIDIDLDDPEVERAATKIQAGYKGMRARK-ELRQHQEEPNDS--------------EPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPAN------------------EVIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQE--------------EINADEAIDIDLEDPDMERAATKIQAGYKGMRARK--------ELRQ----NKEELKDTEA--IDIDLEDPEVERAATKIQAGYKGMRSRKSMR---------EKAAEHQ-DEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREK------------------------------AAEAEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQGMK 675
AE ++DIDL DPEVA AATKIQAG++G KTRK + P+ +ID DP+V KAATKIQA FKG + RK++RK + PE DI DL DPE+ +AATKIQAGY+G++TRR +K V D P +E +DIDL DPEV++AA KIQ+ F+G++ARK A + + E S P IDIDL+DPEVEKAA KIQA +KG++ RR G + P+N++ D +D AA KIQ+ ++G++ RK K +++ E+ IDIDL+DPEV +AA KIQ+ +KG+++RK + +Q + P + E +DIDL DPEVE+AA KIQ+ +KG+++RK + + P N E IDIDL DPE+E+AA KIQA +KG+++R+ + I ++E IDIDL DP++E+AA KIQA +KG+++RK L+Q N + K +E IDIDL DP+VE+AA KIQA +KG+R+RK+ EK E Q +EIDIDL DPE AA +IQAG++G +TRK ++E + ++ +DIDLNDPEV KAAT+IQAG++G + R+ MK
Sbjct: 457 AEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRR--FGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKS--SPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1186 HSP 2 Score: 378.637 bits (971), Expect = 7.850e-104 Identity = 272/738 (36.86%), Postives = 400/738 (54.20%), Query Frame = 0
Query: 436 EPMDIDLEDPEVERAATKIQAGYKGMRARKQLKRDNEEEPANE------------VIDIDLTDPEIEQAATKIQAGYKGMRARRALRQHQEEINADEA-IDI------------------DLEDPDMERAATKIQAGYKGMRARKELRQNKEE------LKDT--------EAIDIDLEDPEVERAATKIQAGYKGMRSRKSMREKAA---------------------EHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMREKAAEAE-----------------QGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ--------------GMKPVEAEPV--PEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKA-----------------EDEIDIDMEDPDEERAATKIQAGYRGMRTRR---------------EMQGRNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK---------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKTLR---ALPEEFPEES-------------PETGLTEDQAATKIQAGYRGMRARKEIVPLNL------------SEMTRDGDPF-------PEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMR 986
E +DIDL DPEV +AATKIQAG+KG + RK++ N ++P ++ DID DP++ +AATKIQA +KG + R+ +R++Q D DI DL DP++E+AATKIQAGYKG++ R+EL+ +E ++D+ E IDIDL DPEVE+AA KIQ+ +KG+R+RKS AA E Q+EIDIDL DPEV AA +IQA +KG+++R+ +K++ + +IDIDLNDPEV KAA +IQ+ ++G+++R+ + P + PV P +D DIDL DPEV +AA KIQ+ +KG+++R+ ++T S ++ E+E+DID+ DP+ E+AA KIQ+ ++G+++R+ + P ++ IDIDL DPEV++AA KIQA +KG++ RK + + ++IDL+DP++ +AA KIQA ++G ++RK + + V+ K P S ++ E + +DIDL+DPDVEKAA KIQA +KG++ RKT A P+E +++ PET QAA KIQAG+RG + RKE V NL SE T+ + E+ + E +DIDL+DPEV +AATKIQAG+KG + RK M+
Sbjct: 460 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEM---NGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRK--FGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETA----QAALKIQAGFRGYKTRKE-VKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMK 1186 HSP 3 Score: 371.703 bits (953), Expect = 1.336e-101 Identity = 281/746 (37.67%), Postives = 404/746 (54.16%), Query Frame = 0
Query: 562 EAIDIDLEDPEVERAATKIQAGYKGMRSRKSM-----------REKAAEHQDEIDIDLEDPEVLMAASRIQAGYKGMRTRKSMR--EKAAEAEQGDIDI------------------DLNDPEVEKAATRIQAGYRGMRTRQGMKPVE------------AEPVP-EDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSA------------------SIKAE--DEIDIDMEDPDEERAATKIQAGYRGMRTRR-------------EMQGRNEIVE--PAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--KLRENQA----------------------LEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRK--TLRALPEEF---------------PEESPETGLTE---DQAATKIQAGYRGMRARK----EIVPLNLSEMTRDGDPFPEEGEP--EAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE---EQP-------------DAMDIDLTDPEVERAATKIQAGYKGMRTRR------------ELKIPYPEEAIH--MADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMD-PQNELVEGELDHAASAAEVDAYGE-PINIDMTAPDMEQAATKIQAGFRGMQARKGIQG 1146
E IDIDL DPEV +AATKIQAG+KG ++RK M R + D DID DP+V AA++IQA +KG + RK +R + A E ++G DI DL+DPEVEKAAT+IQAGY+G++TR+ +K ++ E VP E++ DIDL DPEVE+AA KIQ+ +KG+RAR+ SA S+K E +EIDID+ DP+ E+AA KIQA ++G+++RR M+ V+ P E+ IDIDL DPEV +AA KIQ+ +KG++ RK K + +Q+ ++IDL+DP++A+AA KIQ+ ++G ++RK +++ S NI S ++ VDIDL+DP+VEKAA KIQ+ +KG+K+RK ++ P +E + L + ++AA KIQA ++G+++RK + P S + + P P P E IDIDL+DPEVE+AA KIQA +KG+++RK + K +QP + +DIDLTDP+VE+AA KIQA +KG+R R+ E K EE I + DPE QAA KIQAG+RG +TR+ E+ ESEGD ++ + P E E A+ + E P++ID+ P++ +AATKIQAGF+G + RK ++G
Sbjct: 460 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSP---SLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRK--------------EVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDMKG 1187 HSP 4 Score: 342.813 bits (878), Expect = 3.627e-92 Identity = 261/762 (34.25%), Postives = 379/762 (49.74%), Query Frame = 0
Query: 640 AEQGDIDIDLNDPEVEKAATRIQAGYRGMRTRQ---GMKPVE-----AEPVPEDDTFDIDLEDPEVERAATKIQAGYKGMRARRGLRTSASIKAEDEIDID--------------------MEDPDEERAATKIQAGYRGMRTRREMQG-------------RNEIVEPAEDPIDIDLTDPEVDRAATKIQAGYKGMRERK--------------------------KLRENQALEIDLDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTR-----KTLRALP---EEFPEESPE-TGLTEDQ------------AATKIQAGYRGMRARK-------EIVPLNLSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAE------------------EQPDAMDIDLTDPEVERAATKIQAGYKGMRTRR-------------------ELKIPYPEEA-IHMADPEMEQAATKIQAGYRGMRTRRGMKPIPDVEVVVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG---EPINIDMTAPDMEQAATKIQAGFRGMQARKG----------IQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLA-----------ARRPDIET-------------VESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDL 1231
AE IDIDLNDPEV KAAT+IQAG++G +TR+ G +P+ + + DT DID DP+V +AATKIQA +KG + R+ +R + S D D + DP+ E+AATKIQAGY+G++TRRE++ E V E+ IDIDLTDPEV++AA KIQ+ +KG+R RK K + + ++IDL+DP++ +AA KIQA ++G ++R+ + +N N+ ++ ++ +DIDL+DP+V KAA KIQ+ +KG+K+R KT ++ P P SP T ED+ AA KIQ+ ++G+++RK + P N+ E +DIDL+DPEVE+AA KIQ+ +KG+++RK +K+ + +DIDL DPEVE+AA KIQA +KG+++R+ + IP EE I + DPE+E+AA KIQA ++G+++R+ K P + P L S+ VDA E I+ID+T PD+E+AA KIQA F+G++ARK + + E+ DIDLNDPE AA KIQAG+ G +TR+++ +P ET E +E + D E AATKIQAGFKG K R+D+
Sbjct: 457 AEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSMENVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVEEEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRKIKTKQSTQSPPSNIEPSPPPPV---PVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKP---------VAP---------------------LKQPPSSNHVDAKSSEVEEIDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLNDPETAQAALKIQAGFRGYKTRKEVKENLESEGDKTEGEKPSEETKTTEEAKPEEEAKTEKTKEEPVDIDLNDPEVAKAATKIQAGFKGYKVRKDM 1185 HSP 5 Score: 271.166 bits (692), Expect = 1.206e-69 Identity = 217/666 (32.58%), Postives = 335/666 (50.30%), Query Frame = 0
Query: 727 DEIDIDMEDPDEERAATKIQAGYRGMRTRREMQGRNEIVEPAE--------DPIDIDLTDPEVDRAATKIQAGYKGMRERKKLRENQ-ALEID-------------------------LDDPDLAQAATKIQAGYRGHQARKDYVSRREVVSNRKNIPKSLDEFNRNIEDDIVDIDLSDPDVEKAATKIQAGYKGMKTRKT---------LRALPEEFPEESPETGLTEDQ--------------AATKIQAGYRGMRAR----KEIVPLN----LSEMTRDGDPFPEEGEPEAIDIDLSDPEVERAATKIQAGYKGMRTRKSMREKAEEQP-------------------DAMDIDLTDPEVERAATKIQAGYKGMRTRRELKIPYPEEA----------------------IHMADPEMEQAATKIQAGYRGMRTRR-GMKPIPDVEV------VVGEEITPRSESEGDLSKMDPQNELVEGELDHAASAAEVDAYG------------------------EPINIDMTAPDMEQAATKIQAGFRGMQARK-----------------GIQGRVAKAEDAFDIDLNDPEVEAAASKIQAGYMGMRTRR-----DLAARRPDIETVESNEEVIIGSYPEDDETISAATKIQAGFKGMKARRDLSQ 1233
++IDID+ DP+ +AATKIQAG++G +TR+EM G+ + + D DID DP+V +AATKIQA +KG + RK++R+NQ A E D L DP++ +AATKIQAGY+G + R++ S +E S + S++ N E++I+DIDL+DP+VEKAA KIQ+ +KG++ RK+ + + P P + TE Q AA KIQA ++G+++R K+ P N + +T D P E E IDIDL+DPEV +AA KIQ+ +KG+++RK + K + D +DIDL DPEV +AA KIQ+ +KG+++R+ +K ++ I + DPE+E+AA KIQ+ ++G+++R+ G K + V +TP + E D+ DP+ E ++ + +G E I+ID+ P++E+AA KIQA F+G+++RK + + ++ E+ DIDL DP+VE AA KIQA + G+R R+ + A + + +T EE+ I D ET AA KIQAGF+G K R+++ +
Sbjct: 460 EKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQEPKSTSIPVRDSME--NVPTEEEIIDIDLTDPEVEKAAVKIQSSFKGLRARKSKTASAASTSVESEPSNTTPPCPLSVKTEQQEEIDIDLNDPEVEKAAVKIQATFKGLKSRRFGAKKSSPTNNLNNMKPVTPPVDKVPVE---EEIDIDLNDPEVAKAALKIQSTFKGLKSRKFGKNKTSQSSPPINNVNPTPSPPVTAPVEDEIDIDLNDPEVAKAAVKIQSTFKGLKSRK-IKTKQSTQSPPSNIEPSPPPPVPVPTEEEVDIDLNDPEVEKAALKIQSTFKGLKSRKFGAKKSSPTNIENVSPPVPPPPVTPPVKEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGSKKSSPSLVNKIPTPPPTVNIPSEEEIDIDLNDPEVEKAAVKIQATFKGLKSRKFGKSKPVAPLKQPPSSNHVDAKSSEVEE-IDIDLTDPDVEKAAVKIQASFKGLRARKTKNQQEAAPKEAEKKTEAQEEEIDIDLN--DPETAQAALKIQAGFRGYKTRKEVKE 1116 HSP 6 Score: 85.5001 bits (210), Expect = 1.025e-12 Identity = 53/153 (34.64%), Postives = 82/153 (53.59%), Query Frame = 0
Query: 1109 DAYGEPINIDMTAPDMEQAATKIQAGFRGMQARKGIQGRVAKAE-----------DAFDIDLNDPEVEAAASKIQAGYMGMRTRRDLAARRPDIETVESNEEVIIG--------------SYPEDDETISAATKIQAGFKGMKARRDLSQLRQ 1236
+A E I+ID+ P++ +AATKIQAGF+G + RK + G+ ++ D DID NDP+V AA+KIQA + G + R+++ + ET + ++ D E AATKIQAG+KG+K RR+L +++
Sbjct: 456 NAEPEKIDIDLNDPEVAKAATKIQAGFKGYKTRKEMNGKQPLSDKDSRTSGLDKCDTDDIDFNDPDVAKAATKIQANFKGHQVRKEIRKNQSAPETDRGHTDIHCSDDKHVMDDDNNEIDIDLSDPEVEKAATKIQAGYKGLKTRRELKSIQE 608 HSP 7 Score: 77.7962 bits (190), Expect = 2.591e-10 Identity = 51/112 (45.54%), Postives = 70/112 (62.50%), Query Frame = 0
Query: 88 DIDLNDPDVAVAATKIQAGYRGSKARRDMKSRR--------DDESKQSIKIQMPVQTMDDEDE-----GLELDDPEAEEAELDIDLEDPEVANAATKIQAGYRGMKTRKHLR 186
DI+L DP+V AA KIQAG++G KAR+++K + + + +IKIQ + E + + PE ++ E+DIDLEDPEVA AATKIQAG+RG KTRK L+
Sbjct: 147 DINLEDPEVEAAAVKIQAGFKGYKARKEVKESKAEADLELDPNADEAAIKIQAGFRGHKARKEVKAKKEAQEESPEEKKEEVDIDLEDPEVAKAATKIQAGFRGHKTRKELK 258 The following BLAST results are available for this feature:
BLAST of sperm surface protein sp17 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 6
BLAST of sperm surface protein sp17 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
Pagesback to top
BLAST of sperm surface protein sp17 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold101_size371023:74253..81370+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold101_size371023-processed-gene-0.7 ID=snap_masked-scaffold101_size371023-processed-gene-0.7|Name=sperm surface protein sp17|organism=Tigriopus kingsejongensis|type=gene|length=7118bp|location=Sequence derived from alignment at scaffold101_size371023:74253..81370+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'sperm surface protein sp17' has the following synonyms
Add to Basket
|