hypothetical protein DAPPUDRAFT_22132, snap_masked-scaffold103_size370364-processed-gene-2.2 (gene) Tigriopus kingsejongensis
|
Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Match: EMLSAG00000012326 (supercontig:LSalAtl2s:LSalAtl2s8:1477583:1479313:1 gene:EMLSAG00000012326 transcript:EMLSAT00000012326 description:"augustus_masked-LSalAtl2s8-processed-gene-15.1") HSP 1 Score: 407.912 bits (1047), Expect = 7.720e-129 Identity = 212/494 (42.91%), Postives = 315/494 (63.77%), Query Frame = 0
Query: 34 MKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSED-SDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGN---ATLSQDQVQTFIDHAAQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH-----------------KGM--FIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
MK +F +E +L ++L++Y+ +L+ KL++I +Y++E + +K D SD+++ E+I NPIHAYKL+KRFSVDWKK+E ++ D+W E + + +K+L ++ P+++DL+GSAQ+L+R+ DVY+LD+R+L DCLF+GKH FN+G +SRSLEWFE+AW+LAG + N TLS+D+V+ F+++A+Q HD R +L NV SP +++D+ F +LCRGEE R +A +KC+ + + +PYY LHPV +E +H P + ++++V+S+ EM I+++A P LMRSQVQ + +++S RTSKT WLQDSF P++ M+ +V MTGL ++T KE++ELLQ+ N PH KG+ +IGDRIATWM Y+++V +GGRTVFPRLG VE G+AVFW+NL KSGEAD TLHGACP++ G+KW S
Sbjct: 1 MKRIFFLEKELTNLLNEYSIRLKDKLKKIEDYLNEVETSLSDKSELDTSDDDYFERISENPIHAYKLVKRFSVDWKKLEPEVIEDDWPELELKIHKKKLSTIIPKEQDLHGSAQALLRISDVYDLDVRNLSEGIIENRETSAKLSAKDCLFMGKHSFNSGELSRSLEWFEQAWLLAGXKPNLXATTLSRDEVRNFLEYASQEHDLR-------------------------------RDQLRMNVPSPR----SEEDM--FKSLCRGEEHREPQVLANMKCYTTTKTHPYYLLHPVMVEEVNHDPHIVVYYDVISSNEMKVIKELAEPYLMRSQVQSETY--SEVSKTRTSKTGWLQDSFHPIVSKMSFKVNSMTGLNSDTWKEDAELLQIANYINGGHYQPHHDYVIKGVQPDKMIYGNKGIDYYIGDRIATWMVYMNNVISGGRTVFPRLGLGVEPIPGAAVFWYNLKKSGEADDRTLHGACPVLLGSKWVS 455 HSP 2 Score: 265.388 bits (677), Expect = 7.352e-78 Identity = 175/504 (34.72%), Postives = 254/504 (50.40%), Query Frame = 0
Query: 931 MDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFE-------EVVDMEDNSNE--TQPVVPALYSVQRLASTYQHLEADL-----------------------EEDLYEHFATL----DVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGN---QTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLFPTSYAANPNVFQQRRHANEEKSKATRIRAQAKMASHENWLPELSREDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGKR-----------------------TLLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
M ++F LE+EL +++ + L++KLK I YL + E E+ +D+ E ++ + A V+R + ++ LE ++ E+DL+ L DVY++ V LS+G + ++ + A+L A+DCL++GKH FN LS SLEWF+QA +LA + N TL+ + +L+ AS HD + R Q +M +P E+D+ K+LCRGEE R P+ LA + CY + + PYY LHP VE + P I+V+ ++IS NEM VI+++A L R+QV + VSK R S + W+ DS HPI +++ +V+ MTGL NSD KEDAE LQ+ NYINGG Y HHD+ G + +N+V GGRTVFP +G G+ P G+AVFWYNLKKS DD T H ACP+ G+KW
Sbjct: 1 MKRIFFLEKELTNLLNEYSIRLKDKLKKIEDYLNEVETSLSDKSELDTSDDDYFERISENPIHAYKLVKRFSVDWKKLEPEVIEDDWPELELKIHKKKLSTIIPKEQDLHGSAQALLRISDVYDLDVRNLSEGIIENRE----TSAKLSAKDCLFMGKHSFNSGELSRSLEWFEQAWLLAGXKPNLXATTLSRDEVRNFLEYASQEHDLR---------------------------------------RDQLRMN-----VPSPRSEEDMFKSLCRGEEHREPQVLANMKCYTTTKTHPYYLLHPVMVEEVNHDPHIVVYYDVISSNEMKVIKELAEPYLMRSQVQSETYSEVSKTRTSKTGWLQDSFHPIVSKMSFKVNSMTGL--NSDTW-KEDAELLQIANYINGGHYQPHHDYVIKGVQPDKMIYGNKGIDYYIGDRIATWMVYMNNVISGGRTVFPRLGLGVEPIPGAAVFWYNLKKSGEADDRTLHGACPVLLGSKW 453 HSP 3 Score: 169.859 bits (429), Expect = 3.629e-45 Identity = 103/338 (30.47%), Postives = 176/338 (52.07%), Query Frame = 0
Query: 536 MREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDL---DLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGN---RTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
M+ IF LE+ L + + +L+D L++++ YL E + S ++ D NPI ++ VKR + +KK+E + +D + + + ++ S ++P+E+DL G+A+ LLRI DVY++ V +LS+G I + A L+A+DCL++GKH +N LS SLEWF QA +LA + N TL + +L+ ++ HD L+++ Q R + R E+ D+F ++CRG E R P L+ + CYT+ + YYLL P +E + P I+V+ DV++ N+M +++++A+P L
Sbjct: 1 MKRIFFLEKELTNLLNEYSIRLKDKLKKIEDYLNEVETSLSDKSELDTSDDDYFERISENPIHAYKLVKRFSVDWKKLEPEVIEDDWPELELKIHK-KKLSTIIPKEQDLHGSAQALLRISDVYDLDVRNLSEGIIENRETSAKLSAKDCLFMGKHSFNSGELSRSLEWFEQAWLLAGXKPNLXATTLSRDEVRNFLEYASQEHD--------LRRD-------------QLRMNVPSPRSEE-----------------------DMFKSLCRGEEHREPQVLANMKCYTTTKTHPYYLLHPVMVEEVNHDPHIVVYYDVISSNEMKVIKELAEPYL 293 HSP 4 Score: 83.9593 bits (206), Expect = 8.138e-17 Identity = 34/55 (61.82%), Postives = 42/55 (76.36%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
+NNV GGRTVFP +G G+EP G+AVFWYNL K+ D TLHGACP++ G+KW
Sbjct: 399 MNNVISGGRTVFPRLGLGVEPIPGAAVFWYNLKKSGEADDRTLHGACPVLLGSKW 453
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Match: EMLSAG00000009563 (supercontig:LSalAtl2s:LSalAtl2s615:626046:628492:-1 gene:EMLSAG00000009563 transcript:EMLSAT00000009563 description:"maker-LSalAtl2s615-augustus-gene-5.47") HSP 1 Score: 222.631 bits (566), Expect = 1.804e-62 Identity = 151/514 (29.38%), Postives = 253/514 (49.22%), Query Frame = 0
Query: 27 VYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFS--VRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAA--QSHDERVLKGER-------------GPGLFPQPVYVE-----PPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDI-----PRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTK 477
+++S D++ ++ ++ ++KY K+ ES+++R+ Y+ +YN + S D EE + NP++++ L+K+ + D+ + DL + +FS + R + +P DEDL G+A +LIRLQ+VY+++ RD+ DC +G+ +N G R+ W EA +E T+S+ + ++ +A Q H R LK G L+ + +E P L Q+ +D L E+ E N +++ R+ LCRGE + LKC +P+ + P++ E + KP + IFHNV+S E+ I+++A P R+ VQ + G + ++ R SKT+W++ + ++K + +RV +TGL T SE LQ+ N PH + G+RIATW+FY S V GG TVFP L ++ GSAVFW NL+++GE ++ T H A P++ GTK
Sbjct: 10 IFTSNEDLRQXIYTKSVVLQXVEKYIKEEESRIQRLKGYVEQYNKYL---GSADGHEE---DFVSNPLNSFILIKKLTSDFNDVY-DLIKETSNLNKFSENITDSRESNKWPTDEDLKGAASALIRLQEVYKINTRDMRNGRLNGVDYGPQLTAHDCFELGRQTYNEGDHYRTEIWMTEALSRYDEEDEKTVSKGDIYEYLGFSAYVQGHLRRALKFTNLLLKELPNHPRAIGNKLYYENAIMESGGSGPSLDGSQRKGDDGLDELKEDAVLKEGTGSNAEELLYPETVRYQRLCRGESNEKKKFDRDLKCFLDFGKHPFMKIAPIKAEKLYSKPDIYIFHNVISDNEIETIKNMANPRFKRATVQNAKTGELETANYRISKTAWIKSEENDVVKGIYQRVHDVTGLNMET----SEELQVSNYGIGGHYDPHFDFARREEKDAFSSLGTGNRIATWLFYESEVEQGGATVFPVLDLTLWPQKGSAVFWFNLYRNGEGNEDTRHAAXPVLKGTK 512 HSP 2 Score: 137.117 bits (344), Expect = 1.064e-33 Identity = 106/397 (26.70%), Postives = 179/397 (45.09%), Query Frame = 0
Query: 1015 DVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFH--------------DEQLNNPWGHADQLF-----PTSYAANPNVFQQRRHANE--EKSKATRIRAQAKMASHENWL-PELSREDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGV--SKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGKRTLLNDVGGGGR----------------TVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTK 1371
+VY+I ++ G++ D G +L A DC +G+ +NE + W +A + E +T++ +YL ++ E N+P ++L+ S + P++ +R ++ ++ K + + ++ E L PE R + LCRGE + L C+L P+ ++ P K E + +P I +F N+IS NE+ I++MA +RA V G + + RIS + WI + + + + RV +TG L+ E +E LQV NY GG Y H D R ++ + +G G R TVFP++ + P+ GSAVFW+NL ++ ++ T HAA P+ GTK
Sbjct: 131 EVYKINTRDMRNGRLNGVDY----GPQLTAHDCFELGRQTYNEGDHYRTEIWMTEALSRYDEEDEKTVSKGDIYEYLGFSAYVQGHLRRALKFTNLLLKELPNHPRAIGNKLYYENAIMESGGSGPSLDGSQRKGDDGLDELKEDAVLKEGTGSNAEELLYPETVR----YQRLCRGESNEKKKFDRDLKCFLDFGKHPFMKIAPIKAEKLYSKPDIYIFHNVISDNEIETIKNMANPRFKRATVQNAKTGELETANYRISKTAWIKSEENDVVKGIYQRVHDVTG-------LNMETSEELQVSNYGIGGHYDPHFDFARREEKDAFSSLGTGNRIATWLFYESEVEQGGATVFPVLDLTLWPQKGSAVFWFNLYRNGEGNEDTRHAAXPVLKGTK 512 HSP 3 Score: 98.2117 bits (243), Expect = 3.367e-21 Identity = 78/358 (21.79%), Postives = 167/358 (46.65%), Query Frame = 0
Query: 529 VFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQ-DDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRS--------------PWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKS-YVSKTG-----EIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKP 865
+F+S ++R+ + +L+ +E +I++ E ++ + Y++++++ A G + + NP++SF +K+L + V + + + + F++++ D R S P +EDL+GAA L+R+Q+VY+I D+ G++ + LTA DC +G+ YNE + W +A ++E +T+ +YL +A R+ P + +++ + +Q++ +D L++ K V K G E +L P + +CRG + L C+ + + P + E + +P I +F +V++ N+++ ++++A P
Sbjct: 10 IFTSNEDLRQXIYTKSVVLQXVEKYIKEEESRIQRLKGYVEQYNKYLGS----ADGHEEDFVSNPLNSFILIKKLTSDFNDVYDLIKETSNLNKFSENIT-DSRESNKWPTDEDLKGAASALIRLQEVYKINTRDMRNGRLNGVDYGPQLTAHDCFELGRQTYNEGDHYRTEIWMTEALSRYDEEDEKTVSKGDIYEYLGFSAYVQGHLRRALKFTNLLLKELPNHPRAIGNKLYYENAIMESGGSGPSLDGSQRKGDDGLDELKEDAVLKEGTGSNAEELL--YPETVRYQRLCRGESNEKKKFDRDLKCFLDFGKHPFMKIAPIKAEKLYSKPDIYIFHNVISDNEIETIKNMANP 360 HSP 4 Score: 56.6102 bits (135), Expect = 3.707e-8 Identity = 24/48 (50.00%), Postives = 33/48 (68.75%), Query Frame = 0
Query: 873 GGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTK 920
GG TVFP++ + P+ GSAVFW+NL +N + DT H A P++ GTK
Sbjct: 465 GGATVFPVLDLTLWPQKGSAVFWFNLYRNGEGNEDTRHAAXPVLKGTK 512
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Match: EMLSAG00000011700 (supercontig:LSalAtl2s:LSalAtl2s812:79332:86643:-1 gene:EMLSAG00000011700 transcript:EMLSAT00000011700 description:"maker-LSalAtl2s812-augustus-gene-0.6") HSP 1 Score: 192.2 bits (487), Expect = 3.098e-49 Identity = 140/503 (27.83%), Postives = 236/503 (46.92%), Query Frame = 0
Query: 37 VFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDS-------DEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRR-KRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKP-LLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTK-ISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKT------NTMKEESELLQMINLP--------HKGMFI--------------------GDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
VF IE +L +L + K+L+ LE I Y + L R+E + + E+I+GNPI+ Y++++R V+WK E+ L+ + + ++R K+ P +DL +A++L R+ +Y+++ + D L++G+ N +L W A A N S ++ + A + + ++ K + G + Y+ + + ED P LV+ N D F ALCRGE+L S + L C+Y ++G L P+++E+ H +P L+ FH++ + E + + +RS + G+ K ISD+R S W+ D ++ +++++E + GL++ KEE E+LQ+ N H MFI GDR++T+M Y+S V GG T FPRLG + GSAVFWHN+ +SG +D +HGACP++ G+KW
Sbjct: 43 VFKIERELFKVLQERQKQLQDSLELIKEYTRDVESLY---RNEGCWPLESCIESKIFEQIVGNPIYNYQVLRRLIVNWKNTEDSLKKIDIKQIMTDIKRLKKQNGGLPDGKDLQTAAKALNRIHKIYDINTTEFSNGNILGHQTSANLGLRDTLYLGRFAANTDYPGVALNWLNTAQSQANLHPN---SSEEALKQVKLALRQVNRKLSKKPKSMG--DKDPYILGAIPDKT---ED-----------PNLVISNTDR-RNFEALCRGEKLISSFEESKLYCYYDNRGVFGLKLSPLKVEINHREPHLILTFHDLFTQSETHDLIES-----VRSDLTSAGVGTDKTISDMRISSHVWIPDGMLSIVDKISEKIENVIGLRSMAQLDPEAKKEEYEMLQVANYGLGGHYDCHHDSMFIYKEPQFIPKSVEETQSPYITGDRMSTFMIYLSDVQKGGNTAFPRLGVATSPRKGSAVFWHNIKRSGRSDMHMIHGACPVVMGSKW 517 HSP 2 Score: 139.813 bits (351), Expect = 2.803e-33 Identity = 137/516 (26.55%), Postives = 213/516 (41.28%), Query Frame = 0
Query: 927 SSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDME---------DNSNETQPVVPALYSVQ---RLASTYQHLEADLEE------------------------DLYEHFATLD----VYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNN-PWGHADQLFPTSYAANPNVFQQRRHANEEKSKATRIRAQAKMASHENWLPELSREDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPR-ILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDL---LDKEDAEHLQVGNYINGGLYHIHHDHCRAGKR-------------------------TLLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
+S + +VF +EREL +++ + + L++ L+ I Y +D E + E ++ Q V +Y+ Q RL +++ E L++ DL L+ +Y+I E S G + N G R D LY+G+ N +L W A+ A N + A K + LA + +L+ P D+ P A P+ + N S R +ALCRGE+ + ++L+CY ++ +L P KVEI H P IL F ++ +Q+E + + + R L A VG +S RIS+ WI D I ++++++ ++ GL++ + L KE+ E LQV NY GG Y HHD K L+DV GG T FP +G SP GSAVFW+N+K+S D H ACP+ G+KW
Sbjct: 36 NSESLYRVFKIERELFKVLQERQKQLQDSLELIKEYTRDVESLYRNEGCWPLESCIESKIFEQIVGNPIYNYQVLRRLIVNWKNTEDSLKKIDIKQIMTDIKRLKKQNGGLPDGKDLQTAAKALNRIHKIYDINTTEFSNGNILGHQTSANLGLR----DTLYLGRFAANTDYPGVALNWLNTAQSQANLHPNSS---EEALKQVKLALRQVNRKLSKKPKSMGDK-DPYILGAIPD---KTEDPNLVISNTDRRN---------------------FEALCRGEKLISSFEESKLYCYYDNRGVFGLKLSPLKVEINHREPHLILTFHDLFTQSETHDLIESVRSDLTSAGVGTD--KTISDMRISSHVWIPDGMLSIVDKISEKIENVIGLRSMAQLDPEAKKEEYEMLQVANYGLGGHYDCHHDSMFIYKEPQFIPKSVEETQSPYITGDRMSTFMIYLSDVQKGGNTAFPRLGVATSPRKGSAVFWHNIKRSGRSDMHMIHGACPVVMGSKW 517 HSP 3 Score: 98.9821 bits (245), Expect = 7.648e-21 Identity = 88/358 (24.58%), Postives = 162/358 (45.25%), Query Frame = 0
Query: 535 NMREIFALERNLLRTMESHIQKLEDNLREVDTYLKE---FHQATSHIPDMAVGPSDL---DLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILA------EQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVP-YYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQ-ILVFRDVVTPNDM-DLLRDIAKPLLNNVGGGGRTV 877
++ +F +ER L + ++ ++L+D+L + Y ++ ++ P + S + +GNPI ++ ++RL + +K E+SL + +KR ++++ LP+ +DLQ AAK L RI +Y+I + S G I + A+L +D LY+G+ N +L W + A+ A +E + + + Q L+ + + P+ L +P +DPNLV +++ FE A+CRG + + SKLYCY ++ L P ++EI H +P IL F D+ T ++ DL+ + L + G +T+
Sbjct: 39 SLYRVFKIERELFKVLQERQKQLQDSLELIKEYTRDVESLYRNEGCWPLESCIESKIFEQIVGNPIYNYQVLRRLIVNWKNTEDSLKKIDIKQIMTDIKRLKKQNGGLPDGKDLQTAAKALNRIHKIYDINTTEFSNGNILGHQTSANLGLRDTLYLGRFAANTDYPGVALNWLNTAQSQANLHPNSSEEALKQVKLALRQVNRKLSKKPKSMGDKDPYILG----AIPDKTEDPNLVISNT--DRRNFE----------------------------ALCRGEKLISSFEESKLYCYYDNRGVFGLKLSPLKVEINHREPHLILTFHDLFTQSETHDLIESVRSDLTSAGVGTDKTI 362 HSP 4 Score: 64.6994 bits (156), Expect = 2.064e-10 Identity = 25/55 (45.45%), Postives = 36/55 (65.45%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
L++V GG T FP +G P GSAVFW+N+ ++ D +HGACP++ G+KW
Sbjct: 463 LSDVQKGGNTAFPRLGVATSPRKGSAVFWHNIKRSGRSDMHMIHGACPVVMGSKW 517
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Match: EMLSAG00000012413 (supercontig:LSalAtl2s:LSalAtl2s909:41472:46417:1 gene:EMLSAG00000012413 transcript:EMLSAT00000012413 description:"augustus_masked-LSalAtl2s909-processed-gene-0.4") HSP 1 Score: 169.859 bits (429), Expect = 1.419e-44 Identity = 137/502 (27.29%), Postives = 228/502 (45.42%), Query Frame = 0
Query: 25 AEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLR---SVFPRDEDLNGSAQSLIRLQDVYELDIRDL----------------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVLKGERGPGLFPQPVYVEP-------PLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINLPHKGMF----------------IGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
E++SSA +++++ E + ++ Y LE L + + M + D +E NPI A ++KR ++DW KI T+ +RL S FP+++++ G+ + LQ+ Y+LD D D ++GK +N G R+++W A ++ + + + ++ + ++HDE L+ P YV P ++ L+++ R + V ++ ++ +FN LCRGE LRS S LK Y H +PY L P ++EV + +P L + + + E R+ A L RS G+ +++ RTSK +WL + + K +TKR+E TGL T+ +E Q+ N G++ +GDR+AT M Y+S V AGG T FP LG V G+AVFW+N+ K+G D + HG CP++ G+KW
Sbjct: 26 GELFSSARAIESLYKKEIQINKDIELYINNLEEVLLSLKSTMKSI--------TPDVRKEIHP---NNPISATMVLKRCALDWIKIT--------VLTKLIFENERLLEELSQFPKEKEMYGAMNGIFLLQETYDLDPLDFSNGIIQNKYMTPITVDIQLKIDDMEYLGKLSYNRGYYDRAVDWIMAA-LMKNNKNKDSSNYIRLNKTLSTLMRAHDE-FLEKRGSPAGSDWRTYVTPFNERLAKKKKYKRALKKNNGNRRYKMVPILGRRLNPREKWEQFNRLCRGENLRSPSDEVQLKSFYLHHNDPYLKLGPFKVEVNNIEPSLIVIKDFIFDREGESYRNYAEDKLSRS-FSLSNDGNRQVTLTRTSKQTWLFEKNLTISKIITKRIELATGLVAETLYG-NEPYQVANYGLGGVYNSHMDSGGTIMPEFNELGDRVATVMAYLSDVGAGGGTTFPSLGXKVTPEKGAAVFWYNIDKNGMQDYNMFHGGCPVLTGSKW 504 HSP 2 Score: 134.806 bits (338), Expect = 5.755e-33 Identity = 121/495 (24.44%), Postives = 207/495 (41.82%), Query Frame = 0
Query: 922 GEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDMEDNSN-----------------ETQPVVPALYSVQRLASTYQHL--EADLEEDLYEHFATLDVYEIGVPELSQGKMGLKDAI-LNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQL---NNPWGHADQLFPTSYAANPNVFQQRRHANEEKSKATRIRAQA----KMASHENWLPELSREDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRA----QVGARWLGGVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHC--------RAGKRT-----LLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
GE++SS+ ++ ++ E ++ + ++ LEE L + + +K V E + N + + ++ +RL E ++ + F + Y++ + S G + K + +L D Y+GK +N +++W A ++ + + N K L HDE L +P G + + T + ++ + A ++ + R + ++ E W + LCRGE R+P QL + H NDPY +L P KVE+ + P ++V ++ I E ++ A L R+ G R V+ R S TW+ + + I + + R+ TGL + + E QV NY GG+Y+ H D G R L+DVG GG T FP +G ++PE G+AVFWYN+ K+ M D H CP+ G+KW
Sbjct: 26 GELFSSARAIESLYKKEIQINKDIELYINNLEEVLLSLKSTMKSITPDVRKEIHPNNPISATMVLKRCALDWIKITVLTKLIFENERLLEELSQFPKEKEMYGAMNGIFLLQETYDLDPLDFSNGIIQNKYMTPITVDIQLKIDDMEYLGKLSYNRGYYDRAVDWIMAA-LMKNNKNKDSSNYIRLNKTLSTLMRAHDEFLEKRGSPAGSDWRTYVTPFNERLAKKKKYKRALKKNNGNRRYKMVPILGRRLNPREKW--------EQFNRLCRGENLRSPSDEVQLKSFYLHHNDPYLKLGPFKVEVNNIEPSLIVIKDFIFDREGESYRNYAEDKLSRSFSLSNDGNR---QVTLTRTSKQTWLFEKNLTISKIITKRIELATGLVAETLYGN----EPYQVANYGLGGVYNSHMDSGGTIMPEFNELGDRVATVMAYLSDVGAGGGTTFPSLGXKVTPEKGAAVFWYNIDKNGMQDYNMFHGGCPVLTGSKW 504 HSP 3 Score: 84.7297 bits (208), Expect = 7.154e-17 Identity = 78/346 (22.54%), Postives = 150/346 (43.35%), Query Frame = 0
Query: 527 GEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQ-----LVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFR---SPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAK 864
GE+FSSA + ++ E + + +E +I LE+ L + + +K S PD+ ++ NPIS+ +KR + + K+ +++ R P+E+++ GA G+ +Q+ Y++ D S G I + L D Y+GK YN +++W A ++ + + + K L HDE SP G + P+ + + ++K + ++ N+ V G L + FN +CRG R+P+ +L + H ND Y L P ++E+ +++P ++V +D + + + R+ A+
Sbjct: 26 GELFSSARAIESLYKKEIQINKDIELYINNLEEVLLSLKSTMK------SITPDVR---KEIHPNNPISATMVLKRCALDWIKITVLTKLIFENE------RLLEELSQFPKEKEMYGAMNGIFLLQETYDLDPLDFSNGIIQNKYMTPITVDIQLKIDDMEYLGKLSYNRGYYDRAVDWI-MAALMKNNKNKDSSNYIRLNKTLSTLMRAHDEFLEKRGSPAGSDWRTYVTPFNER---LAKKKKYKRALKKNNGNRRYKMVPILGRR-LNPREKWEQFNRLCRGENLRSPSDEVQLKSFYLHHNDPYLKLGPFKVEVNNIEPSLIVIKDFIFDREGESYRNYAE 351 HSP 4 Score: 73.559 bits (179), Expect = 1.934e-13 Identity = 28/55 (50.91%), Postives = 39/55 (70.91%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
L++VG GG T FP +G + PE G+AVFWYN++KN D + HG CP++ G+KW
Sbjct: 450 LSDVGAGGGTTFPSLGXKVTPEKGAAVFWYNIDKNGMQDYNMFHGGCPVLTGSKW 504
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Match: EMLSAG00000005013 (supercontig:LSalAtl2s:LSalAtl2s260:584077:587279:-1 gene:EMLSAG00000005013 transcript:EMLSAT00000005013 description:"maker-LSalAtl2s260-snap-gene-6.18") HSP 1 Score: 137.887 bits (346), Expect = 2.529e-34 Identity = 114/438 (26.03%), Postives = 202/438 (46.12%), Query Frame = 0
Query: 8 VTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVLKGERGPGLFPQPVYV---EPPLVQRQKLQEDL-----------HQRLIENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQD-------SFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINLPHKGMF 412
+ ++++ + S ++ E++SS+ + + + + L + + Y ++L ++ ++N++S Y S+ + I NPI+A+ L+KR S +W KI + + +E +E F+ L S P D+ G+A L LQ+ Y LDI DL D +GK FN V R+ EW + A ++ ++ + Q++ I + HD+ +L+ P YV EP L + K + L +L+ + E+ +FNALCRGE++R L C Y H +PY L P ++E + P + ++H+ L E+ + + PLL RSQ GK+ S ++S RTSK +WL D + + L ++ R+ + T L + K+ E Q+ N G++
Sbjct: 2 ILLIILIYFCEYSTPSKIELFSSSDSIGRLANVNSRLNNKIQIYLEELNQQISILDNFLSTYYDEYNLTESQ------ADTYISNPINAFTLIKRTSHEWLKIHDSIY-NETSENMFT-EISELLSGLPNSGDIEGAANGLFLLQETYNLDI-DLFAKENNAALNGNDMSLMGKLAFNKQVYDRAYEWLISSLKKAIEDNDSVETISQIKKDIKTVIKYHDQ-ILETRGSPRGSTWRTYVNPLEPKLASKNKYKNALKANNGSMSYKMEYKLLTELKDYEIT-------DQFNALCRGEDIRDPIDDKDLSCSYLHFNDPYLKLGPFKLENLNSYPFIGVYHDFLYDNEIESFKSHSRPLLERSQHSGKK-NSNEVSFKRTSKQTWLVDAETQNNNTVNTLANKISNRISYATKLAVKS-KKGGEDYQIANYGMGGVY 420 HSP 2 Score: 99.3673 bits (246), Expect = 1.042e-21 Identity = 79/352 (22.44%), Postives = 159/352 (45.17%), Query Frame = 0
Query: 528 EVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFR---SPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEII-LKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLLNNVGGGGR 875
E+FSS+ ++ + + L ++ ++++L + +D +L ++ ++ +D + NPI++F +KR + K+ +S+ + ++ + LP D++GAA GL +Q+ Y + + ++ + A+L D +GK +N+++ + EW + A ++ + ++ +K + ++HD+ SP G + P +P L + K N + N + SY + + LK D FNA+CRG + R P L C H ND Y L P ++E + P I V+ D + N+++ + ++PLL G+
Sbjct: 20 ELFSSSDSIGRLANVNSRLNNKIQIYLEELNQQISILDNFLSTYYDEY----NLTESQADTYISNPINAFTLIKRTSHEWLKIHDSIYNETSENMFTEISELLSG---LPNSGDIEGAANGLFLLQETYNLDIDLFAKE------NNAALNGNDMSLMGKLAFNKQVYDRAYEWLISSLKKAIEDNDSVETISQIKKDIKTVIKYHDQILETRGSPRGSTWRTYVNPL--EPKLASKNKYKNALKAN---NGSMSYKMEYKLLTELKDYEITDQFNALCRGEDIRDPIDDKDLSCSYLHFNDPYLKLGPFKLENLNSYPFIGVYHDFLYDNEIESFKSHSRPLLERSQHSGK 353 HSP 3 Score: 97.0561 bits (240), Expect = 6.878e-21 Identity = 76/290 (26.21%), Postives = 130/290 (44.83%), Query Frame = 0
Query: 1038 SGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQL---NNPWGHADQLFPTSYAANPNVFQQRRHANEEKSKATRIRAQAKMASHENWLPELSRED--DILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQ-VGARWLGGVSKDRISTSTWISDSH-------HPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGK 1314
+ A L+ D +GK FN+++ + EW + A + + +S +K + +HD+ L +P G + + P + + ++ N K+ + + + L EL + D ALCRGE+ R P L C H NDPY +L P K+E + P I V+ + + NE+ + +R L+R+Q G + VS R S TW+ D+ + + ++++R+S+ T L S K+ E Q+ NY GG+Y H D A +
Sbjct: 152 NNAALNGNDMSLMGKLAFNKQVYDRAYEWLISSLKKAIEDNDSVETISQIKKDIKTVIKYHDQILETRGSPRGSTWRTYVN--PLEPKLASKNKYKNALKANNGSMSYKMEYK----LLTELKDYEITDQFNALCRGEDIRDPIDDKDLSCSYLHFNDPYLKLGPFKLENLNSYPFIGVYHDFLYDNEIESFKSHSRPLLERSQHSGKKNSNEVSFKRTSKQTWLVDAETQNNNTVNTLANKISNRISYATKLAVKS----KKGGEDYQIANYGMGGVYTPHMDSFGANR 431
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Match: EMLSAG00000012938 (supercontig:LSalAtl2s:LSalAtl2s988:173248:175333:-1 gene:EMLSAG00000012938 transcript:EMLSAT00000012938 description:"snap_masked-LSalAtl2s988-processed-gene-1.22") HSP 1 Score: 135.961 bits (341), Expect = 1.368e-33 Identity = 120/482 (24.90%), Postives = 209/482 (43.36%), Query Frame = 0
Query: 22 RAQAEVYSSASDMKTVFGIET----DLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRD--------EDLNGSAQSLIRLQDVYELDIRDL-----------------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATL-----SQD--QVQTFIDHAAQSHDERVLK-GERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRV-EWMTGLKTNTMKEESELLQMINLPHKGMF-------------------------IGDRIATWMFYISHVPAGGRTV 434
R Q +Y+S +M F E D+ ++DK +E++ Y++ Y ++ +DSDE F+ NP++AY L++ +V W +E +Q + E KR+R + R EDL+G++ +L+RL + Y +I D IG N ++ +E A ++G T S D ++ + A HD+++ + G P V + L +++K + + +++I N S ++D + ++ LC GEELRS + L C Y H+G PYY P +IE +P + I H+ +S E + + A P L RS ++G + G+ + D R S+ SW+ + P + +T ++ +++ L +++ SEL Q+ N G + GDR+AT M Y+S V GG T
Sbjct: 34 RLQKTIYTSMYEMSKFFEREKQYIEDIQTVIDKKLVDMETQ-GSFGAYLASYEDIV--GLQDDSDEAFL----NNPLNAYNLIRHVAVGWNVVESSIQ--RYKEKHPGQLPKRVRKLLSRSKREHLPGAEDLDGASTALVRLHEYYNFNITSFVEEGVIETKDGERFETTGELSVWDIFKIGVKGTNMMILGSGIEIMSHGLAKAKRDGLVTTPPFVDSHDFKTLKNIVKTAKTVHDQKLDRWGPLTPTHSTNYVPYDKRLAKKKKFNKVVSEKMILNNPS---IIDTIQETKQYRKLCNGEELRSPDVLKDLTCRYEHRGLPYYRYGPHKIETVSLRPHIVIIHDFISDEITKEFINSASPNLKRSAMRGFKNGTNAVDDRRVSEQSWINEEV-PAARKLTDQINDYLNLLVDSSI--HSELYQVANYGTAGHYSSHYDQVLMGSSPNDLERRNIFNIKTGDRMATIMGYLSDVEEGGYTT 500 HSP 2 Score: 95.9005 bits (237), Expect = 1.786e-20 Identity = 101/446 (22.65%), Postives = 176/446 (39.46%), Query Frame = 0
Query: 924 VYSSSGGMDQVFGLERELLQ----IMDSQAEALEEKLKHINAYLKDFEEVVDMEDNSNETQPVVP--ALYSVQRLASTYQHLEADLE-----------------------------EDL------------YEHFATLDVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTL-------NVSVAQKYLDLASNFHDEQLNNPWGHADQLFPTSYAANPNVFQQRRHANEEKSKATRIRAQAKMASHENWLPELSREDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLG--GVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAG 1313
+Y+S M + F E++ ++ ++D + +E + AYL +E++V ++D+S+E P A ++ +A + +E+ ++ EDL Y +F E GV E G+ + L D IG N +L + +E A+R+G T + + + A HD++L+ WG T+Y +++ N+ S+ KM + + + +E + LC GEE R+P+ L L C H+ PYYR P K+E RP I++ + IS + A L+R+ + G V R+S +WI++ P R+L D+++ L +S + +E QV NY G Y H+D G
Sbjct: 39 IYTSMYEMSKFFEREKQYIEDIQTVIDKKLVDMETQ-GSFGAYLASYEDIVGLQDDSDEAFLNNPLNAYNLIRHVAVGWNVVESSIQRYKEKHPGQLPKRVRKLLSRSKREHLPGAEDLDGASTALVRLHEYYNFNITSFVEEGVIETKDGER------FETTGELSVWDIFKIGVKGTNMMILGSGIEIMSHGLAKAKRDGLVTTPPFVDSHDFKTLKNIVKTAKTVHDQKLDR-WGPLTPTHSTNYVPYDKRLAKKKKFNKVVSE--------KMILNNPSIIDTIQETKQYRKLCNGEELRSPDVLKDLTCRYEHRGLPYYRYGPHKIETVSLRPHIVIIHDFISDEITKEFINSASPNLKRSAMRGFKNGTNAVDDRRVSEQSWINEE-VPAARKLTDQINDYLNLLVDSSI----HSELYQVANYGTAGHYSSHYDQVLMG 463 HSP 3 Score: 78.9518 bits (193), Expect = 3.807e-15 Identity = 83/363 (22.87%), Postives = 144/363 (39.67%), Query Frame = 0
Query: 524 RVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLD---LGNPISSFHTVKRLGIFYKKVEESLDQ-------DLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVY--------EIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTL-------DVTTAQKYLDLTAEFHDEQFR--SPWGLKQEVFPVPYYKD-------PNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVT 852
R+Q +++S M + F E+ + +++ I K L +++T F + D+ D D L NP+++++ ++ + + + VE S+ + L K + R +R LP EDL GA+ L+R+ + Y E GV + G+ + + L+ D IG N +L +E A+++G T D T + + HD++ P VPY K +V ++ LN DT+ +TK Y +C G E R+P L L C H+ YY P +IE L+P I++ D ++
Sbjct: 34 RLQKTIYTSMYEMSKFFEREKQYIEDIQTVIDK---KLVDMETQ-GSFGAYLASYEDIVGLQDDSDEAFLNNPLNAYNLIRHVAVGWNVVESSIQRYKEKHPGQLPKRVRKLLSRSKREH--LPGAEDLDGASTALVRLHEYYNFNITSFVEEGVIETKDGE--RFETTGELSVWDIFKIGVKGTNMMILGSGIEIMSHGLAKAKRDGLVTTPPFVDSHDFKTLKNIVKTAKTVHDQKLDRWGPLTPTHSTNYVPYDKRLAKKKKFNKVVSEKMILNNPSIIDTIQETKQY------------------RKLCNGEELRSPDVLKDLTCRYEHRGLPYYRYGPHKIETVSLRPHIVIIHDFIS 370
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Match: EMLSAG00000004188 (supercontig:LSalAtl2s:LSalAtl2s221:321106:325044:-1 gene:EMLSAG00000004188 transcript:EMLSAT00000004188 description:"maker-LSalAtl2s221-augustus-gene-3.12") HSP 1 Score: 113.235 bits (282), Expect = 1.321e-25 Identity = 70/223 (31.39%), Postives = 98/223 (43.95%), Query Frame = 0
Query: 1166 LCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGKR----------------TLLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
LC G +FRTP+ A L C+ + +P+ +L P K EI + P + FR ++ E + I + L G + D +TW + + + +L RV + L NS +++ GNY GG Y H D T+L GG TVFP GA PE G+ + W NLK PD+LT HAACP+ FG KW
Sbjct: 458 LCSGVKFRTPKEDADLRCFYADSGNPWLKLGPIKTEILNYDPYVAAFRELLFDEECDNITEYLSPKLDFPP-GRMKSNSLKNDWTMKNTWPVEDDNKVLEKLTLRVEAVMDLLANSK---NNESDKFMCGNYGIGGHYGTHPDFSAYSHEEFKVPGSKVNRISTVMTILQSPSAGGATVFPYTGAYSYPEKGTGIGWXNLKDDGYPDELTRHAACPVLFGQKW 676 HSP 2 Score: 103.99 bits (258), Expect = 1.233e-22 Identity = 65/224 (29.02%), Postives = 100/224 (44.64%), Query Frame = 0
Query: 274 NALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINLPHKGMFIG-------------------DRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
N LC G + R+ A L+C Y+ GNP+ L P++ E+ ++ P + F +L EE I + P L + S + + T K +W + + +++ +T RVE + L N+ ES+ N G + +RI+T M + AGG TVFP GA G+ + W NL G D+ T H ACP++FG KW
Sbjct: 456 NLLCSGVKFRTPKEDADLRCFYADSGNPWLKLGPIKTEILNYDPYVAAFRELLFDEECDNITEYLSPKL---DFPPGRMKSNSLKNDWTMKNTWPVEDDNKVLEKLTLRVEAVMDLLANSKNNESDKFMCGNYGIGGHYGTHPDFSAYSHEEFKVPGSKVNRISTVMTILQSPSAGGATVFPYTGAYSYPEKGTGIGWXNLKDDGYPDELTRHAACPVLFGQKW 676 HSP 3 Score: 73.1738 bits (178), Expect = 4.296e-13 Identity = 80/375 (21.33%), Postives = 170/375 (45.33%), Query Frame = 0
Query: 528 EVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESL---DQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDL-SQGKI-GQL---------VSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQA-RILAEQEGNR-----TLDVTTAQKYLDLT-------AEFHDEQFRSPWGL----KQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNK---TKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLLN 868
++F+S N+++++ ++ +++ E Q LE + + Y+K H+ ++ + LG+PI+S+H +K + + ++ + +++ ++ +++ F K +R ++ LP+ D+QGA+ G+ R+ +YE+ L +G++ G+L S SL A D I ++ +L + + A + L E++ N +VT +D+ E H+ + + + PV K +++ + N+ D L K T K+ E +K N +C G +FRTP + L C+ + + + L P + EI + P + FR+++ + D + + P L+
Sbjct: 162 DLFTSLSNLKDLWINDQEVVQDFEDVAQNLESMAKHLRQYVKS-HKKNGLDSEVNIS----FLGHPINSYHLIKHVSMGWRDIVDNVLNVEKIVREKFDKFHERGEKQ---LPDYYDIQGASFGIARLISLYELNATSLVREGRVVGELGDWQTIYSEPSHVSLNAWDVYSIAAEAFSNRLFNAGYDMLDLALKKLMEEKINPPNDQVVFNVTATHLEMDVVRSSLKKVIEMHNVFLKKRGNISSSHRTHDHPVGLKKPKKKSKKKISKNRFAIMDNLIKDLETYENALKSEETYVKTPIQT---NLLCSGVKFRTPKEDADLRCFYADSGNPWLKLGPIKTEILNYDPYVAAFRELLFDEECDNITEYLSPKLD 525 HSP 4 Score: 60.8474 bits (146), Expect = 2.180e-9 Identity = 26/56 (46.43%), Postives = 33/56 (58.93%), Query Frame = 0
Query: 866 LLNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
+L + GG TVFP GA PE G+ + W NL + PD T H ACP++FG KW
Sbjct: 621 ILQSPSAGGATVFPYTGAYSYPEKGTGIGWXNLKDDGYPDELTRHAACPVLFGQKW 676
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Match: EMLSAG00000004404 (supercontig:LSalAtl2s:LSalAtl2s231:1078605:1080545:-1 gene:EMLSAG00000004404 transcript:EMLSAT00000004404 description:"snap_masked-LSalAtl2s231-processed-gene-10.3") HSP 1 Score: 95.5153 bits (236), Expect = 3.978e-20 Identity = 71/260 (27.31%), Postives = 106/260 (40.77%), Query Frame = 0
Query: 1173 RTPETLAQLH--CYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGVSKD-----RISTSTWISDSHHPIGRRLAD-------------------------------RVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHD-----------------HCRAGKR-----TLLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
R E + + H CYL H ++P+Y+L P K+E+ + P I++FR+ +S E D+A L+R+Q G+ ++ R S W+ +S I + L + R++ G T ++ D AE Q+ NY GG Y +H D + G R L DV GG TVFP + + + G AVFW NL D T H CP+ G+KW
Sbjct: 305 RKKEKVLESHERCYLIHNDNPFYKLLPFKMELLSESPFIVIFRDFMSDVETKHFTDIAGRRLRRSQTGSLTEVEGKEEKGELIRTSKQIWLXESTFNISQFLTEEEIQMITKGTGRPHFPGNYKDHLVILNEIGFRITKRIGRATKLNVEDAWSAEAYQIANYGLGGQYDVHFDARGHFEYPPVDEITRFGYENYGDRLATFMVYLRDVPMGGATVFPKINIYSNAQRGDAVFWINLLSDGTLDTRTLHGGCPVLVGSKW 564 HSP 2 Score: 80.8777 bits (198), Expect = 1.270e-15 Identity = 34/65 (52.31%), Postives = 42/65 (64.62%), Query Frame = 0
Query: 414 GDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
GDR+AT+M Y+ VP GG TVFP++ A G AVFW NL G D TLHG CP++ G+KW
Sbjct: 500 GDRLATFMVYLRDVPMGGATVFPKINIYSNAQRGDAVFWINLLSDGTLDTRTLHGGCPVLVGSKW 564 HSP 3 Score: 58.5362 bits (140), Expect = 1.097e-8 Identity = 27/55 (49.09%), Postives = 33/55 (60.00%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
L +V GG TVFP I + G AVFW NL + T D TLHG CP++ G+KW
Sbjct: 510 LRDVPMGGATVFPKINIYSNAQRGDAVFWINLLSDGTLDTRTLHGGCPVLVGSKW 564
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Match: EMLSAG00000004330 (supercontig:LSalAtl2s:LSalAtl2s227:607006:610705:1 gene:EMLSAG00000004330 transcript:EMLSAT00000004330 description:"maker-LSalAtl2s227-augustus-gene-6.6") HSP 1 Score: 91.2781 bits (225), Expect = 4.615e-19 Identity = 120/494 (24.29%), Postives = 204/494 (41.30%), Query Frame = 0
Query: 924 VYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDMEDNSNETQPVVPALYSVQRLASTYQHLEADLEEDLYEHF-ATLDVYEIGVPELSQGKMGLKDAILN----SGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLFPTSYAANPNVFQQRRHANEEKSKATRIRAQA------------KMASHENWLPELSR-EDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEI-AHDRPRILV--FRNIISQNEMNVIQDMARHTLQRAQVG-ARWLGGVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGKRTLLND---------------------VGGGGRTVFPLVG--AGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
V+ +S G+ +F E L++ + + E L E+ +L++ E N + + P L ++Q S+ H++ + LD Y+ + + QG + +D L+ RL QD L++ K F+ +S+ RV + + + + S +K + + N H ++ G +++P Y + Q+++ + K T K+ E + E + ++ + +CR EE PE+ ++ C L H NDPY RL P K EI + D PR+++ ++I E N+ + R L + W K++ + HPI RL+ ++ H T LK D+ + QV NY GGL +H D + T++ND V GG T F G + P GSA W+NL K+ T+H CP+ GTKW
Sbjct: 25 VFENSKGLKDIFNFEISLVESLRNTREILSER----RNFLQNLE---------NSSHILSPNLLNLQ--MSSVNHIDPFHYDGASRGLLVLLDTYKFDLRGMLQGIV--RDQSLSIELLGKERLTLQDLLHLSKKAFDYNWYDSSIALL---RVXLKAHSDPSTD-SYREKLVQI-HNIHLKKNLKRVGKNFKVYP--YMIDSQTLQKKKKQPKVIGKTTFYNRNVSHIPPPAQLDVQKLKESELAMIEFTDLAEEEFRRICR-EEISPPES-SERKCILLHHNDPYLRLGPFKQEIISEDPPRMILHWLKDIKFNQEYNL--QILRGELNKFDPSFPDWY----KEKEPEDIYGFQIVHPILYRLSLKIEHATRLKVT----DRWSSSAYQVTNYGLGGLCELHIDPHGMLRGTMVNDDRVSLFKTGDMILTLMGWLDDVEAGGSTAFIYPGREGILKPRKGSAAIWFNLHKNGKKSFSTSHGGCPIIGGTKW 482 HSP 2 Score: 67.0106 bits (162), Expect = 2.376e-11 Identity = 95/417 (22.78%), Postives = 156/417 (37.41%), Query Frame = 0
Query: 142 NGSAQSLIRLQDVYELDIRDL-----------------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVLKGER--GPGLFPQPVYVEPPLVQRQKLQ------EDLHQRLIENVTSP-----------ELVVDNQDDIPR--FNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDS----------FDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-----------PH---KGMFI----------GDRIATWMFYISHVPAGGRTVF--PRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
+G+++ L+ L D Y+ D+R + D L + K F+ W++ + L A S ++ + Q H+ + K + G P ++ +Q++K Q + R + ++ P EL + D+ F +CR EE+ S + KC H +PY L P + E+ P I H ++DI +Q Q +++ S W ++ P++ ++ ++E T LK T + S Q+ N PH +G + GD I T M ++ V AGG T F P ++ GSA W NLHK+G+ ST HG CPI+ GTKW
Sbjct: 91 DGASRGLLVLLDTYKFDLRGMLQGIVRDQSLSIELLGKERLTLQDLLHLSKKAFD-------YNWYDSSIALLRVXLKAH-SDPSTDSYREKLVQIHNIHLKKNLKRVGKNFKVYPYMIDSQTLQKKKKQPKVIGKTTFYNRNVSHIPPPAQLDVQKLKESELAMIEFTDLAEEEFRRICR-EEI-SPPESSERKCILLHHNDPYLRLGPFKQEIISEDPPRMILH---------WLKDIK-----FNQEYNLQILRGELNKFDPSFPDWYKEKEPEDIYGFQIVHPILYRLSLKIEHATRLKV-TDRWSSSAYQVTNYGLGGLCELHIDPHGMLRGTMVNDDRVSLFKTGDMILTLMGWLDDVEAGGSTAFIYPGREGILKPRKGSAAIWFNLHKNGKKSFSTSHGGCPIIGGTKW 482 HSP 3 Score: 53.9138 bits (128), Expect = 2.589e-7 Identity = 26/57 (45.61%), Postives = 33/57 (57.89%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIG--AGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
L++V GG T F G ++P GSA W+NL+KN T HG CPI+ GTKW
Sbjct: 426 LDDVEAGGSTAFIYPGREGILKPRKGSAAIWFNLHKNGKKSFSTSHGGCPIIGGTKW 482
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Match: EMLSAG00000010983 (supercontig:LSalAtl2s:LSalAtl2s73:252044:320772:-1 gene:EMLSAG00000010983 transcript:EMLSAT00000010983 description:"maker-LSalAtl2s73-augustus-gene-3.4") HSP 1 Score: 84.7297 bits (208), Expect = 4.746e-17 Identity = 84/351 (23.93%), Postives = 152/351 (43.30%), Query Frame = 0
Query: 1017 YEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCF---NEKLLSNSLE------WFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPW-GHADQLFPTSYAANPNVFQQRRHANEE-KSKATRIRAQAKMASHENWLPELSREDDILKALCRGEE----FRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGVSKDRISTSTWISDSHHPIGRRLA---DRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGKRTLLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNL 1349
YE+ +++ G++G + + ARL A++ I K + + S+ +E W + L+ + Q + + + L + HD+ PW G + +P N F QR + N + SK R + + N R+ ++CRGEE R + + CY+ H ++P+ L PAK+EI ++ P ++ Q+ + S DR ++ W+ +S +P +++ ++V+H++ K +D ++E Q+G Y GG+Y H D L++V GGG F +G P+ GS+VFW+NL
Sbjct: 156 YELDPYDMAMGRLGTR----QTNARLSAEELYEIVKILYYGSDRTYYSSGIEYALAITWLEGVIRLSFEDDLQDVFIEEIKTLLLEIQDEHDKHFQEPWTGQSLLQYP-----NEEFFVQRFNGNRDMTSKEMRKLEKETVNKFSNQRSPWGRD---FFSICRGEEQKWAKRASSSSSDQRCYVKHFSNPFLLLQPAKIEILNENPTLV-------------------------QIHDK-----SYDRTQSNAWLQNSEYPNLPKISKYFEKVTHLSISKLFND-GSMVESETYQIGVYSPGGVYLPHMDTFNTDNE--LSNVDGGG-XAFTELGKVAYPKMGSSVFWFNL 460 HSP 2 Score: 78.9518 bits (193), Expect = 3.791e-15 Identity = 104/465 (22.37%), Postives = 183/465 (39.35%), Query Frame = 0
Query: 46 DILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEW-----AETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------------DCLFIG--KHCFNNGV-ISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIP---RFNALCRGEEL----RSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKE-----ESELLQM-------INLPHKGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGE 460
++L+KY K N + G ++++ED + +E ++ NP+H + L R + I ++L + ET+ + +++ + +DL G +LIR+Q YELD D+ L+ G + +++G+ + ++ W E L+ ++ + ++++T + HD+ + G L P E VQR D+ + + + + + P F ++CRGEE R+ S + +C+ H NP+ L P +IE+ + P L H+ K D RT +WLQ+S P + ++K E +T L + + ESE Q+ + LPH F D +S+V GG F LG GS+VFW NL G+
Sbjct: 54 NLLNKYLNK---------NTLESLIGTQSQEKNED-EAYGIEGLVANPLHVFSLFYRILILLPIISKELNEIKKEPGIPQETKDFLDSPQIKDI--SQDDLVGVTFALIRIQQAYELDPYDMAMGRLGTRQTNARLSAEELYEIVKILYYGSDRTYYSSGIEYALAITWLEGVIRLSFEDDLQDVFIEEIKTLLLEIQDEHDKHFQEPWTGQSLLQYPN--EEFFVQRFNGNRDMTSKEMRKLEKETVNKFSNQRSPWGRDFFSICRGEEQKWAKRASSSSSDQRCYVKHFSNPFLLLQPAKIEILNENPTLVQIHD--------KSYD------------------------RTQSNAWLQNSEYPNLPKISKYFEKVTHLSISKLFNDGSMVESETYQIGVYSPGGVYLPHMDTFNTDN------ELSNVDGGG-XAFTELGKVAYPKMGSSVFWFNLFSDGK 465 HSP 3 Score: 63.1586 bits (152), Expect = 3.026e-10 Identity = 70/286 (24.48%), Postives = 123/286 (43.01%), Query Frame = 0
Query: 580 DMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPE--EEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCY---NEKLLSHSLE------WFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNK-TKSYVSKTGEIILKGAPSADVFNAMCRGTE----FRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRD 849
D A G L + NP+ F R+ I + + L++ ++ +D S + + ++DL G L+RIQ YE+ D++ G++G + A L+A++ I K Y + S +E W L+ ++ + + + + L + HD+ F+ PW Q + P + QR N+ + K K V+K + +P F ++CRG E R + S CY H ++ + LLQPA+IEI + P ++ D
Sbjct: 79 DEAYGIEGL-VANPLHVFSLFYRILILLPIISKELNEIKKEPGIPQETKDFLDSPQIKDISQDDLVGVTFALIRIQQAYELDPYDMAMGRLGTRQTNARLSAEELYEIVKILYYGSDRTYYSSGIEYALAITWLEGVIRLSFEDDLQDVFIEEIKTLLLEIQDEHDKHFQEPWT-GQSLLQYP---NEEFFVQRFNGNRDMTSKEMRKLEKETVNKFSN---QRSPWGRDFFSICRGEEQKWAKRASSSSSDQRCYVKHFSNPFLLLQPAKIEILNENPTLVQIHD 356
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Match: gi|82082587|sp|Q5ZLK5.1|P4HA2_CHICK (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor) HSP 1 Score: 223.787 bits (569), Expect = 2.204e-61 Identity = 159/521 (30.52%), Postives = 259/521 (49.71%), Query Frame = 0
Query: 11 CLILFGSK-CSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDE--RVLK-GERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLI-ENVTSPELVVDNQ------DDIPR---FNALCRGEELR-SGSYVATLKCHYSHQGN--PYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINLPHKGMFI-----------------GDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
CL+ F S W A+AE ++S M + E DLV L +Y + E+KL +I ++ + + L + S+ E + +P++AYKL+KR + DW ++E + D F R FP +ED G+A++L+RLQD Y+LD L DC +GK +N+G ++ W E+A + + T+S+ ++ ++ +A + R ++ R L L +KL E ++ + V + E VV + D +P + ALCRGE ++ + L C Y H GN P+ + P + E P + +++V+S EE+ KI+ +A P L R+ V+ + G ++ R SK+SWL++ DP++ + +R++ +TGL T +ELLQ+ N G + G+R+AT++ Y+S V AGG TVFP GA++ G+AVFW+NL +SGE D T H ACP++ G KW S
Sbjct: 6 CLVFFTSAFLIWHAEAEFFTSIGQMTDLIYAEKDLVQSLKEYIRAEETKLSQIKSWAEKMDVLTSKSTSDP------EGYLAHPVNAYKLVKRLNTDWLELENLVLQD--TTNGFITNLTIQRQFFPTEEDETGAAKALMRLQDTYKLDPETLSRGNLPGTKYRSSLTVSDCFGMGKTAYNDGDYYHTVLWMEQALKQHDEGEDTTVSKVEILDYLSYAVFQFGDLHRAMELTRRLISLDSTHERAGSNLRYFEKLLEKEREKPSNKTVATTEPVVQSGAYERPLDYLPERDIYEALCRGEGVKMTPRRQKRLFCRY-HDGNRNPHLLIAPFKEEDEWDSPHIVRYYDVMSDEEIEKIKQLAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVAKVNQRMQQITGLTVKT----AELLQVANYGMGGQYEPHFDFSRRPFDSTLKSEGNRLATFLNYMSDVEAGGATVFPDFGAAIWPKKGTAVFWYNLFRSGEGDYRTRHAACPVLVGCKWVS 513 HSP 2 Score: 150.214 bits (378), Expect = 1.360e-36 Identity = 150/525 (28.57%), Postives = 226/525 (43.05%), Query Frame = 0
Query: 913 CPIMFGTKW------GEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDMEDNSNETQP------VVPALYSVQRLASTYQHLEADLEEDLYEHFAT-------------------------LDVYEIGVPELSQGKM-GLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLFP---TSYAANPNV------FQQRRHANEEKSKATRIRAQAKMASHENWLPELSREDDILKALCRGEEFR-TPETLAQLHC-YLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLG--GVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCR---------AGKR--TLLN---DVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
C + F + + E ++S G M + E++L+Q + A E KL I K + E +D+ + + + P V A V+RL + + LE + +D F T D Y++ LS+G + G K + L DC +GK +N+ +++ W +QA + + T++ YL A F L+ +L T A N+ ++ R K+ AT + ++E L L E DI +ALCRGE + TP +L C Y +P+ + P K E D P I+ + +++S E+ I+ +A+ L RA V G V+ R+S S+W+ + P+ ++ R+ +TGL + AE LQV NY GG Y H D R G R T LN DV GG TVFP GA I P+ G+AVFWYNL +S D T HAACP+ G KW
Sbjct: 6 CLVFFTSAFLIWHAEAEFFTSIGQMTDLIYAEKDLVQSLKEYIRAEETKLSQI----KSWAEKMDVLTSKSTSDPEGYLAHPVNAYKLVKRLNTDWLELENLVLQDTTNGFITNLTIQRQFFPTEEDETGAAKALMRLQDTYKLDPETLSRGNLPGTK-----YRSSLTVSDCFGMGKTAYNDGDYYHTVLWMEQALKQHDEGEDTTVSKVEILDYLSYAV-FQFGDLHRAMELTRRLISLDSTHERAGSNLRYFEKLLEKEREKPSNKTVAT-TEPVVQSGAYERPLDYLP-ERDIYEALCRGEGVKMTPRRQKRLFCRYHDGNRNPHLLIAPFKEEDEWDSPHIVRYYDVMSDEEIEKIKQLAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVAKVNQRMQQITGLTVKT-------AELLQVANYGMGGQYEPHFDFSRRPFDSTLKSEGNRLATFLNYMSDVEAGGATVFPDFGAAIWPKKGTAVFWYNLFRSGEGDYRTRHAACPVLVGCKW 511 HSP 3 Score: 120.168 bits (300), Expect = 8.458e-27 Identity = 90/358 (25.14%), Postives = 170/358 (47.49%), Query Frame = 0
Query: 525 VQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNK-----TKSYVSKTGEIILKGA--------PSADVFNAMCRGTEFR-TPAYLSKLYC-YTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
+ E F+S G M ++ E++L+++++ +I+ E L ++ ++ ++ TS + + L +P++++ VKRL + ++E + QD + F ++ R+ P EED GAAK L+R+QD Y++ LS+G + ++SLT DC +GK YN+ H++ W QA ++ + T+ YL A F L + + + + +R N + FE L K + V+ T ++ GA P D++ A+CRG + TP +L+C Y + + L+ P + E P I+ + DV++ +++ ++ +AKP L
Sbjct: 19 AEAEFFTSIGQMTDLIYAEKDLVQSLKEYIRAEETKLSQIKSWAEKMDVLTSK----STSDPEGYLAHPVNAYKLVKRLNTDWLELENLVLQDTTNGFITNLTIQRQ---FFPTEEDETGAAKALMRLQDTYKLDPETLSRGNLPGTKYRSSLTVSDCFGMGKTAYNDGDYYHTVLWMEQALKQHDEGEDTTVSKVEILDYLSY-AVFQFGDLHRAMELTRRLISL------DSTHERAGSNLRYFEKLLEKEREKPSNKTVATTEPVVQSGAYERPLDYLPERDIYEALCRGEGVKMTPRRQKRLFCRYHDGNRNPHLLIAPFKEEDEWDSPHIVRYYDVMSDEEIEKIKQLAKPKL 362 HSP 4 Score: 67.0106 bits (162), Expect = 6.470e-10 Identity = 28/55 (50.91%), Postives = 37/55 (67.27%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
+++V GG TVFP GA I P+ G+AVFWYNL ++ D T H ACP++ G KW
Sbjct: 457 MSDVEAGGATVFPDFGAAIWPKKGTAVFWYNLFRSGEGDYRTRHAACPVLVGCKW 511
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Match: gi|20455169|sp|O15460.1|P4HA2_HUMAN (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor) HSP 1 Score: 223.402 bits (568), Expect = 2.623e-61 Identity = 166/528 (31.44%), Postives = 264/528 (50.00%), Query Frame = 0
Query: 6 LWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAW--ILAGQEGNATLSQDQVQTFIDHAA-QSHD-ERVLK-GERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELV---------VDNQDDIPRFNALCRGEELR-SGSYVATLKCHYSHQGN--PYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
LWV+ L+ + S QAE ++S M + E +LV L +Y E+KL +I ++ ++ L + ++ E + +P++AYKL+KR + DW +E+ + D A F R FP DED G+A++L+RLQD Y LD + DC +G+ +N G ++ W E+ + AG+E AT ++ QV ++ +A Q D R L+ R L P L ++L E+ ++ + N T EL VD + + +LCRGE ++ + L C Y H GN P + P + E P + +++V+S EE+ +I++IA P L R+ V+ + G ++ R SK+SWL++ DP++ + +R++ +TGL T +ELLQ+ N PH K + G+R+AT++ Y+S V AGG TVFP LGA++ G+AVFW+NL +SGE D T H ACP++ G KW S
Sbjct: 3 LWVSALLMAWFGVLSC-VQAEFFTSIGHMTDLIYAEKELVQSLKEYILVEEAKLSKIKSWANKMEALTSKSAAD------AEGYLAHPVNAYKLVKRLNTDWPALEDLVLQDSAA--GFIANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKYQAMLSVDDCFGMGRSAYNEGDYYHTVLWMEQVLKQLDAGEE--ATTTKSQVLDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVKLTPRRQKRLFCRY-HHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKT----AELLQVANYGVGGQYEPHFDFSRNDERDTFKHLGTGNRVATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVS 514 HSP 2 Score: 160.614 bits (405), Expect = 4.410e-40 Identity = 150/509 (29.47%), Postives = 226/509 (44.40%), Query Frame = 0
Query: 922 GEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDMEDNSNETQPVVP--ALYSVQRLASTYQHLEADLEED----------LYEHFATLDVYEIGVPELSQGKMGLKDAI-LNSG-------------ARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQ--KYLDLASNFHDEQLNNPWGHADQLF---PTSYAANPNVFQQRRHANEEKSKATRIRAQAKMASHE-------NWLPELSREDDILKALCRGEEFR-TPETLAQLHCYLSHQN-DPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLG--GVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGKR-------------TLLN---DVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
E ++S G M + E+EL+Q + E KL I ++ E + E P A V+RL + + LE + +D + F D EIG ++ M L+D L+ G A L DC +G+ +NE +++ W +Q VL + + + + +Q YL A F L+ +L P+ A N+ + EE+ K + +A++A+ E ++LPE D+ ++LCRGE + TP +L C H N P + P K E D P I+ + +++S E+ I+++A+ L RA V G V+ R+S S+W+ + P+ R+ R+ H+TGL + AE LQV NY GG Y H D R +R T LN DV GG TVFP +GA I P+ G+AVFWYNL +S D T HAACP+ G KW
Sbjct: 21 AEFFTSIGHMTDLIYAEKELVQSLKEYILVEEAKLSKIKSWANKMEALTSKSAADAEGYLAHPVNAYKLVKRLNTDWPALEDLVLQDSAAGFIANLSVQRQFFPTDEDEIGA---AKALMRLQDTYRLDPGTISRGELPGTKYQAMLSVDDCFGMGRSAYNEGDYYHTVLWMEQ--VLKQLDAGEEATTTKSQVLDYLSYAV-FQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPE----RDVYESLCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKT-------AELLQVANYGVGGQYEPHFDFSRNDERDTFKHLGTGNRVATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKW 512 HSP 3 Score: 117.857 bits (294), Expect = 5.115e-26 Identity = 101/375 (26.93%), Postives = 178/375 (47.47%), Query Frame = 0
Query: 509 MKSRVFELIL--LGCLLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQ--KYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDP-------NLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGA---PSADVFNAMCRGTEFR-TPAYLSKLYCYTSHQNDLYYLL-QPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
MK V L++ G L VQ E F+S G+M ++ E+ L+++++ +I E L ++ ++ + TS A G L +P++++ VKRL + +E+ + QD F ++ R+ P +ED GAAK L+R+QD Y + +S+G++ QA L+ DC +G+ YNE H++ W Q +L + + T +Q YL A F L + + + DP NL + L ++R + N+T++ ++ I + P DV+ ++CRG + TP +L+C H N LL P + E P I+ + DV++ +++ +++IAKP L
Sbjct: 1 MKLWVSALLMAWFGVLSCVQAEFFTSIGHMTDLIYAEKELVQSLKEYILVEEAKLSKIKSWANKMEALTSKSAADAEGY----LAHPVNAYKLVKRLNTDWPALEDLVLQDSAAGFIANLSVQRQ---FFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKYQAMLSVDDCFGMGRSAYNEGDYYHTVLWMEQ--VLKQLDAGEEATTTKSQVLDYLSY-AVFQLGDLHRALELTRRLLSL----DPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKL 361 HSP 4 Score: 67.3958 bits (163), Expect = 5.182e-10 Identity = 28/55 (50.91%), Postives = 38/55 (69.09%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
+++V GG TVFP +GA I P+ G+AVFWYNL ++ D T H ACP++ G KW
Sbjct: 458 MSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKW 512
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Match: gi|90111077|sp|P54001.2|P4HA1_RAT (RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; Flags: Precursor) HSP 1 Score: 219.164 bits (557), Expect = 7.131e-60 Identity = 157/528 (29.73%), Postives = 244/528 (46.21%), Query Frame = 0
Query: 5 LLWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAA-QSHDERVLKGERGPGLFPQP---------VYVEPPLVQRQKLQEDLHQRLIENVTSPE---LVVDNQDDIPRFNALCRGEELR-SGSYVATLKCHYSHQGN--PYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
++W + + +CS A ++S M + E DLV L Y K E KLE+I + + + L + E +G+P++A+KLMKR + +W ++E + D F R FP DED G+A++L RLQD Y LD + DC +GK + + W E+A + + +T+ + V ++ +A Q D L P VY E + + + + + T+P+ + VD + ++ LCRGE ++ + L C Y H GN P + L P + E KP + FH+++S E+ ++D+A P L R+ V + G + R SK++WL DP++ + R++ +TGL +T +E LQ+ N PH + + G+RIATW+FY+S V AGG TVFP +GASV G+AVFW+NL SGE D ST H ACP++ G KW S
Sbjct: 1 MIWGVLMMGILLPQCS--AHPGFFTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDP------EGFVGHPVNAFKLMKRLNTEWSELENLILKD--MSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTTPKKKGIAVDYLPERQKYEMLCRGEGIKMTPRRQKRLFCRY-HDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLSRATVHDPETGKLTTAQYRVSKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEE----LQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVS 513 HSP 2 Score: 160.999 bits (406), Expect = 3.288e-40 Identity = 142/506 (28.06%), Postives = 220/506 (43.48%), Query Frame = 0
Query: 925 YSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDM--EDNSNETQPVVPALYSVQRLASTYQHLEADLEEDLYEHFAT-------------------------LDVYEIGVPELSQGKM-GLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLF--PTSYAANPN------VFQQRRHANEEKS--KATRIRAQAKMASHENWLPELSREDDILKALCRGEEFR-TPETLAQLHC-YLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGVS--KDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCR-----------AGKRT-----LLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
++S G M + E++L+ + +A E+KL+ I + + + + +D V A ++RL + + LE + +D+ + F + D Y + +S+G + G+K L A+DC +GK + E ++ W +QA + E T++ YL A + L P AN N + + + AN+ S ++ + K ++LPE + + LCRGE + TP +L C Y +P + L PAK E D+PRI+ F +IIS E+ +++D+A+ L RA V G ++ + R+S S W+S P+ R+ R+ +TGL D AE LQV NY GG Y H D R G R ++DV GG TVFP VGA + P+ G+AVFWYNL S D T HAACP+ G KW
Sbjct: 22 FTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVKHKSF-----LTAEDCFELGKVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTTPKKKGIAVDYLPERQK----YEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLSRATVHDPETGKLTTAQYRVSKSAWLSGYEDPVVSRINMRIQDLTGL-------DVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKW 511 HSP 3 Score: 126.716 bits (317), Expect = 7.118e-29 Identity = 91/369 (24.66%), Postives = 184/369 (49.86%), Query Frame = 0
Query: 513 VFELILLGCLL---RVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPN--------LVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFR-TPAYLSKLYC-YTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLLN 868
++ ++++G LL F+S G M ++ E++L+ +++ +I+ ED L ++ + ++ + TS A + +G+P+++F +KRL + ++E + +D+ D F ++ R+ P +ED GAAK L R+QD Y + +S+G + + ++ LTA+DC +GK Y E H+ W QA + E+ T+D + YL + ++ K+ + P ++ N ++ + K N+ D ++ K+ K G I + P + +CRG + TP +L+C Y + ++L PA+ E +P+I+ F D+++ ++++++D+AKP L+
Sbjct: 2 IWGVLMMGILLPQCSAHPGFFTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTS----TATKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQ---YFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQ-KTTPKKKG-IAVDYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLS 361 HSP 4 Score: 67.781 bits (164), Expect = 4.278e-10 Identity = 27/55 (49.09%), Postives = 37/55 (67.27%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
+++V GG TVFP +GA + P+ G+AVFWYNL + D T H ACP++ G KW
Sbjct: 457 MSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKW 511
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Match: gi|2498741|sp|Q60716.1|P4HA2_MOUSE (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor) HSP 1 Score: 219.164 bits (557), Expect = 7.697e-60 Identity = 165/531 (31.07%), Postives = 261/531 (49.15%), Query Frame = 0
Query: 20 SWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAW--ILAGQEGNATLSQDQVQTFIDHAA--------------------QSHD---------ERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELR-SGSYVATLKCHYSHQGN--PYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
SW QAE ++S M + E DLV L +Y E+KL +I ++ S+ L ++ E + +P++AYKL+KR + DW + + + D A F R FP DED +G+A++L+RLQD Y+LD + DC +G+ +N G ++ W E+ + AG+E AT+++ V ++ +A SH+ ER+L+ ERG L Q + L QE+L++R + + PE V + +LCRGE ++ + L C Y H GN P + P + E P + +++V+S EE+ +I++IA P L R+ V+ + G ++ R SK+SWL++ DP++ + +R++ +TGL T +ELLQ+ N PH K + G+R+AT++ Y+S V AGG TVFP LGA++ G+AVFW+NL +SGE D T H ACP++ G KW S
Sbjct: 19 SW-VQAEFFTSIGHMTDLIYAEKDLVQSLKEYILVEEAKLAKIKSWASKMEALTSRSAADP------EGYLAHPVNAYKLVKRLNTDWPALGDLVLQD--ASAGFVANLSVQRQFFPTDEDESGAARALMRLQDTYKLDPDTISRGELPGTKYQAMLSVDDCFGLGRSAYNEGDYYHTVLWMEQVLKQLDAGEE--ATVTKSLVLDYLSYAVFQLGDLHRAVELTRRLLSLDPSHERAGGNLRYFERLLEEERGKSLSNQ---TDAGLAT----QENLYERPTDYL--PERDV--------YESLCRGEGVKLTPRRQKKLFCRY-HHGNRVPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKT----AELLQVANYGMGGQYEPHFDFSRSDDEDAFKRLGTGNRVATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVS 516 HSP 2 Score: 159.458 bits (402), Expect = 1.028e-39 Identity = 149/504 (29.56%), Postives = 221/504 (43.85%), Query Frame = 0
Query: 922 GEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDMEDNSNETQPVVP--ALYSVQRLASTYQHLEADLEEDLYEHF-ATLDVYEIGVPE------LSQGKMGLKDA------ILNSG--------ARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLF---PTSYAANPNVFQQRRHANEEKSKATRIRAQAKMASHEN-------WLPELSREDDILKALCRGEEFR-TPETLAQLHCYLSHQND-PYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLG--GVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRA-----------GKR--TLLN---DVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
E ++S G M + E++L+Q + E KL I ++ E + E P A V+RL + + L + +D F A L V P ++ M L+D ++ G A L DC +G+ +NE +++ W +Q + T+ S+ YL A F L+ +L P+ A N+ R EE+ K+ + A +A+ EN +LPE D+ ++LCRGE + TP +L C H N P + P K E D P I+ + +++S E+ I+++A+ L RA V G V+ R+S S+W+ + P+ R+ R+ H+TGL + AE LQV NY GG Y H D R+ G R T LN DV GG TVFP +GA I P+ G+AVFWYNL +S D T HAACP+ G KW
Sbjct: 23 AEFFTSIGHMTDLIYAEKDLVQSLKEYILVEEAKLAKIKSWASKMEALTSRSAADPEGYLAHPVNAYKLVKRLNTDWPALGDLVLQDASAGFVANLSVQRQFFPTDEDESGAARALMRLQDTYKLDPDTISRGELPGTKYQAMLSVDDCFGLGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATVTKSLVLDYLSYAV-FQLGDLHRAVELTRRLLSLDPSHERAGGNLRYFERLLEEERGKSLSNQTDAGLATQENLYERPTDYLPE----RDVYESLCRGEGVKLTPRRQKKLFCRYHHGNRVPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKT-------AELLQVANYGMGGQYEPHFDFSRSDDEDAFKRLGTGNRVATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKW 514 HSP 3 Score: 116.316 bits (290), Expect = 1.658e-25 Identity = 93/361 (25.76%), Postives = 170/361 (47.09%), Query Frame = 0
Query: 519 LGCLLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDP-------NLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGA---PSADVFNAMCRGTEFR-TPAYLSKLYCYTSHQNDLYYLL-QPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
G L VQ E F+S G+M ++ E++L+++++ +I E L ++ ++ + TS A P L +P++++ VKRL + + + + QD F ++ R+ P +ED GAA+ L+R+QD Y++ +S+G++ QA L+ DC +G+ YNE H++ W Q + T+ + YL A F L + + + DP NL + L ++R + N+T + ++ + + P DV+ ++CRG + TP KL+C H N + LL P + E P I+ + DV++ +++ +++IAKP L
Sbjct: 15 FGVLSWVQAEFFTSIGHMTDLIYAEKDLVQSLKEYILVEEAKLAKIKSWASKMEALTSR---SAADPEGY-LAHPVNAYKLVKRLNTDWPALGDLVLQDASAGFVANLSVQRQ---FFPTDEDESGAARALMRLQDTYKLDPDTISRGELPGTKYQAMLSVDDCFGLGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATVTKSLVLDYLSY-AVFQLGDLHRAVELTRRLLSL----DPSHERAGGNLRYFERLLEEERGKSLSNQTDAGLATQENLYERPTDYLPERDVYESLCRGEGVKLTPRRQKKLFCRYHHGNRVPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKL 363 HSP 4 Score: 67.3958 bits (163), Expect = 5.622e-10 Identity = 28/55 (50.91%), Postives = 38/55 (69.09%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
+++V GG TVFP +GA I P+ G+AVFWYNL ++ D T H ACP++ G KW
Sbjct: 460 MSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKW 514
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Match: gi|32171589|sp|Q20065.1|P4HA2_CAEEL (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor) HSP 1 Score: 215.312 bits (547), Expect = 1.631e-58 Identity = 148/505 (29.31%), Postives = 245/505 (48.51%), Query Frame = 0
Query: 23 AQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRS--VFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHA--AQSHDERVLK-GERGPGLFPQPVYVEPPLVQRQKLQEDLHQRL-----IENVTSPELVVDNQDDIPRFNALCRGE-ELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
A A+++++ +D++ + G E D+ I+D+Y + ++L+ + Y EY + +E EF + NPI+AY L+KR + +WKK+E + ++ + ++ R+RS FP +EDL+G+A +L+RLQD Y LD DL D +G+ +N L W + A + E T+ + ++ ++ ++ Q + R L +R + P P K ED+ Q + + + + D + + ALCRGE + L+C Y + P+ L P+++E+ PL +F NV+ E+ I+++A P L R+ VQ + G + + R SK++WL+ DP+I + +R+E T L T SE LQ+ N PH K + G+RIAT +FY+S GG TVF LG +V + A+FW+NL + GE D T H ACP++ G KW S
Sbjct: 14 AHADLFTAIADLQHMLGAEKDVTTIIDQYIEAERARLDDLRRYAHEY--VHRNAHAESVGPEF----VTNPINAYLLIKRLTTEWKKVENIMLNNKASTFLKNITDNRVRSEVKFPGEEDLSGAATALLRLQDTYSLDTLDLSNGIIGGEKVSNKLSGHDTFEVGRSAYNQKDYYHCLMWMQVALVKIENENPPTIEEWEILEYLAYSLYQQGNVRRALSLTKRLAKIAPN----HPRAKGNVKWYEDMLQGKDMVGDLPPIVNKRVEYDGIVERDAYEALCRGEIPPVEPKWKNKLRC-YLKRDKPFLKLAPIKVEILRFDPLAVLFKNVIHDSEIEVIKELASPKLKRATVQNSKTGELEHATYRISKSAWLKGDLDPVIDRVNRRIEDFTNLNQAT----SEELQVANYGLGGHYDPHFDFARKEEKNAFKTLNTGNRIATVLFYMSQPERGGATVFNHLGTAVFPSKNDALFWYNLRRDGEGDLRTRHAACPVLLGVKWVS 503 HSP 2 Score: 135.191 bits (339), Expect = 1.273e-31 Identity = 101/360 (28.06%), Postives = 174/360 (48.33%), Query Frame = 0
Query: 517 ILLGCLLR--VQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIV-LPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILK-----GAPSADVFNAMCRG-TEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
+LL CLL ++F++ +++ + E+++ ++ +I+ L ++ Y E+ +H +VGP + NPI+++ +KRL +KKVE + + F K++ +R RS V P EEDL GAA LLR+QD Y + DLS G IG L+ D +G+ YN+K H L W A + E E T++ +YL + + R L + + + PN R N K +ED L + K V I+ K G D + A+CRG P + +KL CY ++ + L P ++EI P ++F++V+ +++++++++A P L
Sbjct: 4 VLLVCLLAGLAHADLFTAIADLQHMLGAEKDVTTIIDQYIEAERARLDDLRRYAHEYVHRNAHAE--SVGPEFVT--NPINAYLLIKRLTTEWKKVENIMLNNKASTFLKNITDNRVRSEVKFPGEEDLSGAATALLRLQDTYSLDTLDLSNGIIGGEKVSNKLSGHDTFEVGRSAYNQKDYYHCLMWMQVALVKIENENPPTIEEWEILEYLAYSL-YQQGNVRRALSLTKRLAKIA----PN--HPRAKGNVKWYEDML-QGKDMVGDLPPIVNKRVEYDGIVERDAYEALCRGEIPPVEPKWKNKLRCYLK-RDKPFLKLAPIKVEILRFDPLAVLFKNVIHDSEIEVIKELASPKL 350 HSP 3 Score: 129.413 bits (324), Expect = 9.018e-30 Identity = 129/534 (24.16%), Postives = 213/534 (39.89%), Query Frame = 0
Query: 915 IMFGTKWGEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDF------EEVVDMEDNSNETQPVVPALYSVQRLASTYQHLEADL-------------------------EEDLYEHFATL----DVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAERE---------------------GNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLFPTSYAAN-PNVFQQRRHANEEKSKATRIRAQAKMASHENWLPELSREDDILKALCRGE-EFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGV--SKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGKRTLLNDVGGGGR----------------TVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
++ G +++++ + + G E+++ I+D EA +L + Y ++ E V E +N + A ++RL + ++ +E + EEDL L D Y + +LS G +G + +L D +G+ +N+K + L W Q A V E E GN +S+ ++ +A N + N W + D L + P + +R ++ + E D +ALCRGE P+ +L CYL ++ P+ +L P KVEI P ++F+N+I +E+ VI+++A L+RA V G + + RIS S W+ P+ R+ R+ T L ++ +E LQV NY GG Y H D R ++ + G R TVF +G + P A+FWYNL++ D T HAACP+ G KW
Sbjct: 9 LLAGLAHADLFTAIADLQHMLGAEKDVTTIIDQYIEAERARLDDLRRYAHEYVHRNAHAESVGPEFVTNP----INAYLLIKRLTTEWKKVENIMLNNKASTFLKNITDNRVRSEVKFPGEEDLSGAATALLRLQDTYSLDTLDLSNGIIGGEKV----SNKLSGHDTFEVGRSAYNQKDYYHCLMWMQVALVKIENENPPTIEEWEILEYLAYSLYQQGNVRRALSLTKRLAKIAPNHPRAKGNVKW-YEDMLQGKDMVGDLPPIVNKR-------------------VEYDGIV-----ERDAYEALCRGEIPPVEPKWKNKLRCYLK-RDKPFLKLAPIKVEILRFDPLAVLFKNVIHDSEIEVIKELASPKLKRATVQNSKTGELEHATYRISKSAWLKGDLDPVIDRVNRRIEDFTNL-------NQATSEELQVANYGLGGHYDPHFDFARKEEKNAFKTLNTGNRIATVLFYMSQPERGGATVFNHLGTAVFPSKNDALFWYNLRRDGEGDLRTRHAACPVLLGVKW 501
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Match: gi|20455506|sp|Q60715.2|P4HA1_MOUSE (RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; Flags: Precursor) HSP 1 Score: 213.001 bits (541), Expect = 9.550e-58 Identity = 155/528 (29.36%), Postives = 242/528 (45.83%), Query Frame = 0
Query: 5 LLWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAA-QSHDERVLKGERGPGLFPQP---------VYVEPPLVQRQKLQEDLHQRLIENVTSPE---LVVDNQDDIPRFNALCRGEELR-SGSYVATLKCHYSHQGN--PYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
++WV + + + A ++S M + E DLV L Y K E KLE+I + + + L + E +G+P++A+KLMKR + +W ++E + D F R FP DED G+A++L RLQD Y LD + DC +GK + + W E+A + +T+ + V ++ +A Q D L P VY E + + + + + T+P+ + VD + ++ LCRGE ++ + L C Y H GN P + L P + E KP + FH+++S E+ ++D+A P L R+ + G+ + R SK++WL DP++ + R++ +TGL +T +E LQ+ N PH + + G+RIATW+FY+S V AGG TVFP +GASV G+AVFW+NL SGE D ST H ACP++ G KW S
Sbjct: 1 MIWVVLMMAILLPQS--LAHPGFFTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDP------EGFVGHPVNAFKLMKRLNTEWSELENLILKD--MSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVQHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALTQLEEGELSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTAPKKKGIAVDYLPERQKYEMLCRGEGIKMTPRRQKRLFCRY-HDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPVTGALETVHYRISKSAWLSGYEDPVVSRINMRIQDLTGLDVSTAEE----LQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVS 513 HSP 2 Score: 161.384 bits (407), Expect = 2.378e-40 Identity = 141/505 (27.92%), Postives = 218/505 (43.17%), Query Frame = 0
Query: 925 YSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDM--EDNSNETQPVVPALYSVQRLASTYQHLEADLEEDLYEHFAT-------------------------LDVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLF--PTSYAANPN------VFQQRRHANEEKS--KATRIRAQAKMASHENWLPELSREDDILKALCRGEEFR-TPETLAQLHC-YLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGVSK--DRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCR-----------AGKRT-----LLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
++S G M + E++L+ + +A E+KL+ I + + + + +D V A ++RL + + LE + +D+ + F + D Y + +S+G + + + L A+DC +GK + E ++ W +QA E T++ YL A + L P AN N + + + AN+ S ++ + A K ++LPE + + LCRGE + TP +L C Y +P + L PAK E D+PRI+ F +IIS E+ +++D+A+ L+RA + G + RIS S W+S P+ R+ R+ +TGL D AE LQV NY GG Y H D R G R ++DV GG TVFP VGA + P+ G+AVFWYNL S D T HAACP+ G KW
Sbjct: 22 FTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNL----PGVQHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALTQLEEGELSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTAPKKKGIAVDYLPERQK----YEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPVTGALETVHYRISKSAWLSGYEDPVVSRINMRIQDLTGL-------DVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFRELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKW 511 HSP 3 Score: 123.25 bits (308), Expect = 8.558e-28 Identity = 93/364 (25.55%), Postives = 178/364 (48.90%), Query Frame = 0
Query: 530 FSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPN--------LVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFR-TPAYLSKLYC-YTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL------NNVGGGGRTV 877
F+S G M ++ E++L+ +++ +I+ ED L ++ + ++ + TS A + +G+P+++F +KRL + ++E + +D+ D F ++ R+ P +ED GAAK L R+QD Y + +S+G + + ++ LTA+DC +GK Y E H+ W QA E+ T+D + YL + ++ K+ + P ++ N ++ + K N+ D ++ K+ K G I + P + +CRG + TP +L+C Y + ++L PA+ E +P+I+ F D+++ ++++++D+AKP L N V G TV
Sbjct: 22 FTSIGQMTDLIHNEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTS----TATKDPEGFVGHPVNAFKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQ---YFPNDEDQVGAAKALFRLQDTYNLDTNTISKGNLPGVQHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALTQLEEGELSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQ-KTAPKKKG-IAVDYLPERQKYEMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPVTGALETV 376 HSP 4 Score: 67.781 bits (164), Expect = 3.925e-10 Identity = 27/55 (49.09%), Postives = 37/55 (67.27%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
+++V GG TVFP +GA + P+ G+AVFWYNL + D T H ACP++ G KW
Sbjct: 457 MSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKW 511
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Match: gi|20455505|sp|Q10576.2|P4HA1_CAEEL (RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; AltName: Full=Protein dumpy-18; Flags: Precursor) HSP 1 Score: 211.075 bits (536), Expect = 6.930e-57 Identity = 148/506 (29.25%), Postives = 244/506 (48.22%), Query Frame = 0
Query: 23 AQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQV----------QTFIDHAAQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDLHQRL--IENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
A A++++S +DM+ + E ++ ILDKY E +L ++ EY+ K++E S E + K I NPI+A+ L+KR DWK+IE + ++ S+ +P +DL+G+A L+RLQD Y LD +DL DC I + +N ++ W EEA G E T+ + + Q + HA + +E P + E L Q + D+ + L I+N P+ V+ N + + ALCR E S ++ L C+Y + P+ P+++E+ PL +F +V+S +E+A I+++A P L R+ V G + R SK++WL++ +++ + KR+ +MT L+ T +E LQ+ N PH + + G+RIAT +FY+S GG TVF +++ T A+FW+NL+K G+ + T H ACP++ G KW S
Sbjct: 14 AVADLFTSIADMQNLLETERNIPKILDKYIHDEEERLVQLKKLSEEYS-----KKNEISIENGL-KDITNPINAFLLIKRKIFDWKEIESKMNANKAGNVVSSITDDSYGVRYPTADDLSGAAIGLLRLQDTYRLDTKDLADGKIYADQGNYTFSAKDCFEIARAAYNEHDFYHTVMWMEEAQRRLGDEVEPTVEVEDILEYLAFALYKQNNLKHALKLTEELYKMNPTHPRAKGNVKWYEDLLEQEGVRRSDMRKNLPPIQN-RRPDSVLGNTERT-MYEALCRNEVPVSQKDISRLYCYYK-RDRPFLVYAPIKVEIKRFNPLAVLFKDVISDDEVAAIQELAKPKLARATVHDSVTGKLVTATYRISKSAWLKEWEGDVVETVNKRIGYMTNLEMETAEE----LQIANYGIGGHYDPHFDHAKKEESKSFESLGTGNRIATVLFYMSQPSHGGGTVFTEAKSTILPTKNDALFWYNLYKQGDGNPDTRHAACPVLVGIKWVS 506 HSP 2 Score: 135.576 bits (340), Expect = 1.230e-31 Identity = 125/507 (24.65%), Postives = 218/507 (43.00%), Query Frame = 0
Query: 917 FGTKWGEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEV--VDMEDNSNETQPVVPALYSVQRLASTYQHLEADLEEDLYEHFA---TLDVYEIGVP---ELSQGKMGLKDAILNSGARLD-------------------AQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLF---PTSYAANPNVFQQRRHANEEKSKATRIRAQAKMASHENWLPEL---SREDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGV--SKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGKRTLLNDVGGGGR----------------TVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
G ++++S M + ER + +I+D EE+L + +++ + + +E+ + + A ++R ++ +E+ + + + T D Y + P +LS +GL L RLD A+DC I + +NE +++ W ++A+ E T+ V +YL A + L + ++L+ PT A NV +E + + +R + +N P+ + E + +ALCR E + + +++L+CY ++ P+ P KVEI P ++F+++IS +E+ IQ++A+ L RA V G + + RIS S W+ + + + R+ +MT L+ E AE LQ+ NY GG Y H DH + + +G G R TVF + I P A+FWYNL K + T HAACP+ G KW
Sbjct: 11 IGYAVADLFTSIADMQNLLETERNIPKILDKYIHDEEERLVQLKKLSEEYSKKNEISIENGLKDITNPINAFLLIKRKIFDWKEIESKMNANKAGNVVSSITDDSYGVRYPTADDLSGAAIGL--LRLQDTYRLDTKDLADGKIYADQGNYTFSAKDCFEIARAAYNEHDFYHTVMWMEEAQRRLGDEVEPTVEVEDILEYLAFAL-YKQNNLKHALKLTEELYKMNPTHPRAKGNVKWYEDLLEQEGVRRSDMRKN--LPPIQNRRPDSVLGNTERTMYEALCRNEVPVSQKDISRLYCYYK-RDRPFLVYAPIKVEIKRFNPLAVLFKDVISDDEVAAIQELAKPKLARATVHDSVTGKLVTATYRISKSAWLKEWEGDVVETVNKRIGYMTNLEM-------ETAEELQIANYGIGGHYDPHFDHAKKEESKSFESLGTGNRIATVLFYMSQPSHGGGTVFTEAKSTILPTKNDALFWYNLYKQGDGNPDTRHAACPVLVGIKW 504 HSP 3 Score: 132.109 bits (331), Expect = 1.684e-30 Identity = 93/363 (25.62%), Postives = 178/363 (49.04%), Query Frame = 0
Query: 516 LILLGCLLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKT---KSYVSKTGEIILKGAPSA-------DVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLL-QPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
L++L + ++F+S +M+ + ERN+ + ++ +I E+ L + LK+ + S ++++ D+ NPI++F +KR +K++E ++ + + S+ D + P +DL GAA GLLR+QD Y + DL+ GKI + +A+DC I + YNE H++ W +A+ E T++V +YL A + + L +E++ + N R N K +ED L + +S + K I P + ++ A+CR + +S+LYCY ++ D +L+ P ++EI P ++F+DV++ +++ ++++AKP L
Sbjct: 5 LLVLATIGYAVADLFTSIADMQNLLETERNIPKILDKYIHDEEERLVQ----LKKLSEEYSKKNEISIENGLKDITNPINAFLLIKRKIFDWKEIESKMNANKAGNVVSSITDDSY-GVRYPTADDLSGAAIGLLRLQDTYRLDTKDLADGKIYADQGNYTFSAKDCFEIARAAYNEHDFYHTVMWMEEAQRRLGDEVEPTVEVEDILEYLAF-ALYKQNNLKHALKLTEELYKM------NPTHPRAKGNVKWYEDLLEQEGVRRSDMRKNLPPIQNRRPDSVLGNTERTMYEALCRNEVPVSQKDISRLYCY--YKRDRPFLVYAPIKVEIKRFNPLAVLFKDVISDDEVAAIQELAKPKL 353
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Match: gi|2507090|sp|P13674.2|P4HA1_HUMAN (RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; Flags: Precursor >gi|75061858|sp|Q5RAG8.1|P4HA1_PONAB RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; Flags: Precursor) HSP 1 Score: 209.92 bits (533), Expect = 1.056e-56 Identity = 152/510 (29.80%), Postives = 235/510 (46.08%), Query Frame = 0
Query: 23 AQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAA-QSHDERVLKGERGPGLFPQP---------VYVEPPLVQRQKLQEDLHQRLIENVTSPE---LVVDNQDDIPRFNALCRGEELR-SGSYVATLKCHYSHQGN--PYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
A ++S M + E DLV L Y K E KLE+I + + + L + E +G+P++A+KLMKR + +W ++E + D F R FP DED G+A++L+RLQD Y LD + DC +GK + + W E+A + +T+ + V ++ +A Q D L P Y E + + + + + + T+P+ + VD + ++ LCRGE ++ + L C Y H GN P + L P + E KP + FH+++S E+ ++D+A P L R+ + G + R SK++WL +P++ + R++ +TGL +T +E LQ+ N PH K + G+RIATW+FY+S V AGG TVFP +GASV G+AVFW+NL SGE D ST H ACP++ G KW S
Sbjct: 17 AHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDP------EGFVGHPVNAFKLMKRLNTEWSELENLVLKD--MSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRY-HDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEE----LQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVS 513 HSP 2 Score: 161.384 bits (407), Expect = 2.148e-40 Identity = 146/507 (28.80%), Postives = 222/507 (43.79%), Query Frame = 0
Query: 925 YSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDM--EDNSNETQPVVPALYSVQRLASTYQHLEADLEEDLYEHFAT-------------------------LDVYEIGVPELSQGKM-GLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQA-RVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLF--PTSYAANPN------VFQQRRHANEEKS--KATRIRAQAKMASHENWLPELSREDDILKALCRGEEFR-TPETLAQLHC-YLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGVSK--DRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCR-----------AGKRT-----LLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
++S G M + E++L+ + +A E+KL+ I + + + + +D V A ++RL + + LE + +D+ + F + D Y + +S+G + G+K L A+DC +GK + E ++ W +QA R L E E + VSV YL A + L P AN N + + + N+ S ++ + K ++LPE + + LCRGE + TP +L C Y +P + L PAK E D+PRI+ F +IIS E+ +++D+A+ L+RA + G + RIS S W+S +P+ R+ R+ +TGL D AE LQV NY GG Y H D R G R ++DV GG TVFP VGA + P+ G+AVFWYNL S D T HAACP+ G KW
Sbjct: 22 FTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSF-----LTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLD-YLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQK----YEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGL-------DVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKW 511 HSP 3 Score: 126.716 bits (317), Expect = 6.443e-29 Identity = 95/369 (25.75%), Postives = 188/369 (50.95%), Query Frame = 0
Query: 513 VFELILLGCLL---RVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQA-RILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPN--------LVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFR-TPAYLSKLYC-YTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
++ ++++G LL F+S G M ++ E++L+ +++ +I+ ED L ++ + ++ + TS A + +G+P+++F +KRL + ++E + +D+ D F ++ R+ P +ED GAAK LLR+QD Y + +S+G + + ++ LTA+DC +GK Y E H+ W QA R L E E + T+D + YL + ++ K+ + P ++ N ++ + K +N+ +D ++ K+ K G + + P + +CRG + TP KL+C Y + ++L PA+ E +P+I+ F D+++ ++++++D+AKP L
Sbjct: 2 IWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTS----TATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQ---YFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEIS-TIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQ-KTTPKKKG-VAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRL 360 HSP 4 Score: 67.781 bits (164), Expect = 4.097e-10 Identity = 27/55 (49.09%), Postives = 37/55 (67.27%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
+++V GG TVFP +GA + P+ G+AVFWYNL + D T H ACP++ G KW
Sbjct: 457 MSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKW 511
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Match: gi|129365|sp|P16924.1|P4HA1_CHICK (RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1) HSP 1 Score: 207.994 bits (528), Expect = 3.047e-56 Identity = 162/513 (31.58%), Postives = 242/513 (47.17%), Query Frame = 0
Query: 25 AEVYSSASDMKTVFGIETDLV-DILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQT--FIDHAA-QSHD--------ERVLK----GERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIP---RFNALCRGEELR-SGSYVATLKC-HYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
+ ++S M + E DLV L Y K ESKLE+I + + + L D+ + E +G+P +A+KLMKR + +W ++E + D F R FP DED G+ ++L+RLQD Y LD L DC +GK + + W E+A + EG + S D+V ++ +A Q D +R+L+ +R G Y+ + K D E+ T E V +D +P ++ LCRGE L+ + L C +Y NP Y L PV+ E KP + F +++S EE+ ++++A P L R+ V + G + R SK++WL P++ + R++ +TGL +T +E LQ+ N PH K + G+RIATW+FY+S V AGG TVFP +GASV G+AVFW+NL SGE D ST H ACP++ G KW S
Sbjct: 2 TDFFTSIGHMTDLINTEKDLVISKLKDYIKAEESKLEQIKKWAEKLDKLT------DTATKDPEGFLGHPANAFKLMKRLNTEWGELESLVLKD--MSDGFISNMTIQRQFFPNDEDQTGARKALLRLQDTYNLDTDTLSRGNLPGVKHKSFLTAEDCFELGKIRYTEADYYHTELWMEQA-LKQLDEGEVS-SADKVYILDYLSYAVYQQGDLSKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTD-----AEDQTDKETEVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSRATVHDPETGKLTTAHYRVSKSAWLSGYESPVVSRINTRIQDLTGLDVSTAEE----LQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVS 495 HSP 2 Score: 160.999 bits (406), Expect = 2.490e-40 Identity = 144/509 (28.29%), Postives = 224/509 (44.01%), Query Frame = 0
Query: 923 EVYSSSGGMDQVFGLEREL-LQIMDSQAEALEEKLKHINAYLKDFEEVVDMEDNSNETQPVVPA--LYSVQRLASTYQHLEADLEEDLYEHFAT-------------------------LDVYEIGVPELSQGKM-GLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQA-RVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLF---PTSYAANPN------VFQQRRHANEEKSKATRIRAQAKMASHENWLPELSREDDILKALCRGEEFR-TPETLAQLHC-YLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGVSKD--RISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCR-----------AGKRT-----LLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
+ ++S G M + E++L + + +A E KL+ I + + +++ D E PA ++RL + + LE+ + +D+ + F + D Y + LS+G + G+K L A+DC +GK + E ++ W +QA + L E E + V + YL A + L+ +L P AN N + + + AN+ + A + +++LPE + + LCRGE + TP +L C Y +P Y L P K E D+PRI+ F +IIS E+ ++++A+ L RA V G ++ R+S S W+S P+ R+ R+ +TGL ++ AE LQV NY GG Y H D R G R ++DV GG TVFP VGA + P+ G+AVFWYNL S D T HAACP+ G KW
Sbjct: 3 DFFTSIGHMTDLINTEKDLVISKLKDYIKAEESKLEQIKKWAEKLDKLTDTATKDPEGFLGHPANAFKLMKRLNTEWGELESLVLKDMSDGFISNMTIQRQFFPNDEDQTGARKALLRLQDTYNLDTDTLSRGNLPGVKHKSF-----LTAEDCFELGKIRYTEADYYHTELWMEQALKQLDEGEVSSADKVYILD-YLSYAV-YQQGDLSKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTDAEDQTDKETEVKKKDYLPERRK----YEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSRATVHDPETGKLTTAHYRVSKSAWLSGYESPVVSRINTRIQDLTGLDVST-------AEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKW 493 HSP 3 Score: 111.694 bits (278), Expect = 4.482e-24 Identity = 93/357 (26.05%), Postives = 166/357 (46.50%), Query Frame = 0
Query: 528 EVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYL---KEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKS----------YVSKTGEIILKG-APSADVFNAMCRGTEFR-TPAYLSKLYC-YTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLLN 868
+ F+S G+M ++ E++L+ I KL+D ++ ++ L K++ + + D A + LG+P ++F +KRL + ++E + +D+ D F ++ R+ P +ED GA K LLR+QD Y + LS+G + + ++ LTA+DC +GK Y E H+ W QA ++ + D YL A + L + + + DP QR N K FE + K K K E+ K P + +CRG + TP +L+C Y + Y+L P + E +P+I+ F D+++ +++ ++++AKP L+
Sbjct: 3 DFFTSIGHMTDLINTEKDLV------ISKLKDYIKAEESKLEQIKKWAEKLDKLTDTATKDPEGFLGHPANAFKLMKRLNTEWGELESLVLKDMSDGFISNMTIQRQ---FFPNDEDQTGARKALLRLQDTYNLDTDTLSRGNLPGVKHKSFLTAEDCFELGKIRYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSY-AVYQQGDLSKAMMLTKRLLEL----DPE--HQRANGNMKYFEYIMAKEKEANKSSTDAEDQTDKETEVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLS 343 HSP 4 Score: 68.1662 bits (165), Expect = 3.344e-10 Identity = 27/55 (49.09%), Postives = 37/55 (67.27%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
+++V GG TVFP +GA + P+ G+AVFWYNL + D T H ACP++ G KW
Sbjct: 439 MSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKW 493
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Match: gi|122144801|sp|Q1RMU3.1|P4HA1_BOVIN (RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; Flags: Precursor) HSP 1 Score: 206.838 bits (525), Expect = 1.318e-55 Identity = 149/510 (29.22%), Postives = 228/510 (44.71%), Query Frame = 0
Query: 23 AQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVL-------------KGERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELR-SGSYVATLKCHYSHQGN--PYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
A ++S M + E DLV L Y K E KLE+I + + + L + E +G+P++A+KLMKR + +W ++E + D F R FP DED G+A++L+RLQD Y LD + DC +GK + + W E+A + +T+ + V ++ +A + + +R G Y+ K D + VD + ++ LCRGE ++ + L C Y H GN P + L P + E KP + FH+++S E+ ++D+A P L R+ + G + R SK++WL +P++ + R++ +TGL +T +E LQ+ N PH K + G+RIATW+FY+S V AGG TVFP +GASV G+AVFW+NL SGE D ST H ACP++ G KW S
Sbjct: 17 AHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDP------EGFVGHPVNAFKLMKRLNTEWSELENLVLKD--MSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGDLPGVKHKSFLTVEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEVSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDANKSSSDDQSDQKTTLKKKGAAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRY-HDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEVVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEE----LQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVLAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVS 513 HSP 2 Score: 162.155 bits (409), Expect = 1.377e-40 Identity = 146/507 (28.80%), Postives = 224/507 (44.18%), Query Frame = 0
Query: 925 YSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDM--EDNSNETQPVVPALYSVQRLASTYQHLEADLEEDLYEHFAT-------------------------LDVYEIGVPELSQGKM-GLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQA-RVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLF--PTSYAANPN------VFQQRRHANEEKS--KATRIRAQAKMASHENWLPELSREDDILKALCRGEEFR-TPETLAQLHC-YLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGVSK--DRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCR-----------AGKRT-----LLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
++S G M + E++L+ + +A E+KL+ I + + + + +D V A ++RL + + LE + +D+ + F + D Y + +S+G + G+K L +DC +GK + E ++ W +QA R L E E + VSV YL A + L P AN N + + + AN+ S ++ + K + ++LPE + + LCRGE + TP +L C Y +P + L PAK E D+PRI+ F +IIS E+ V++D+A+ L+RA + G + RIS S W+S +P+ R+ R+ +TGL ++ AE LQV NY GG Y H D R G R ++DV GG TVFP VGA + P+ G+AVFWYNL S D T HAACP+ G KW
Sbjct: 22 FTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGDLPGVKHKSF-----LTVEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEVSTVDKVSVLD-YLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDANKSSSDDQSDQKTTLKKKGAAVDYLPERQK----YEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEVVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVST-------AEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVLAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKW 511 HSP 3 Score: 123.635 bits (309), Expect = 6.828e-28 Identity = 98/374 (26.20%), Postives = 181/374 (48.40%), Query Frame = 0
Query: 513 VFELILLGCLL---RVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQA-RILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGE--------IILKGA-----PSADVFNAMCRGTEFR-TPAYLSKLYC-YTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
++ ++++G LL F+S G M ++ E++L+ +++ +I+ ED L ++ + ++ + TS A + +G+P+++F +KRL + ++E + +D+ D F ++ R+ P +ED GAAK LLR+QD Y + +S+G + + ++ LT +DC +GK Y E H+ W QA R L E E + T+D + YL + ++ + DP QR N K FE + K K + + + KGA P + +CRG + TP KL+C Y + ++L PA+ E +P+I+ F D+++ ++++++D+AKP L
Sbjct: 2 IWYILVVGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTS----TATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQ---YFPNDEDQVGAAKALLRLQDTYNLDTDTISKGDLPGVKHKSFLTVEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEVS-TVDKVSVLDYLSYAVYQQGDLDKA-----LLLTKKLLELDPE--HQRANGNLKYFEYIMAKEKDANKSSSDDQSDQKTTLKKKGAAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEVVKDLAKPRL 360 HSP 4 Score: 66.6254 bits (161), Expect = 9.133e-10 Identity = 27/55 (49.09%), Postives = 37/55 (67.27%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
+++V GG TVFP +GA + P+ G+AVFWYNL + D T H ACP++ G KW
Sbjct: 457 MSDVLAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKW 511
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Match: gi|1325329337|ref|XP_023346532.1| (prolyl 4-hydroxylase subunit alpha-2-like [Eurytemora affinis]) HSP 1 Score: 389.808 bits (1000), Expect = 2.677e-118 Identity = 210/511 (41.10%), Postives = 284/511 (55.58%), Query Frame = 0
Query: 10 TCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQ-------------------------MINLPHKGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
T + LFG + + Q EV+SS SDM VF +E DLV +L +A+K ++KL KI GNPIH Y ++KR +VD+ +E+++ D+W E V R RL V P+++DL+G+AQ+LIRLQDVYEL+I L DCLFIGKHCFN G++SRS+EWFEEAW LAG+E N T++QD+V + +V RQ ++DD F ALCRGEELR+G + A L C H+G PY +L P Q+E+ H P + +FHN+ S E+ +I+D+ PLL RSQV G + G ++S RTSKT WL DS +P + + +R+E +TGLKTNT +++ELLQ MI LP+ +IGDR+ATWMFY++ V GGRTVFPRL A V+ G+AVFW+NL SG+AD TLHGACP+++GTKW
Sbjct: 6 TIIFLFGLQST---QGEVFSSVSDMSRVFQLERDLVGVLLDFAQKTQNKLN---------------------------KIAGNPIHTYNVVKRLAVDFVSLEKEISFDDWKENSLRVERLRLGGVVPKEKDLHGAAQALIRLQDVYELEISHLVRGRIGDRDTTAKLTAQDCLFIGKHCFNGGILSRSIEWFEEAWYLAGEEQNTTVTQDKVSRNYE-----------------------------VVDRQ---------------------GDEDDAANFAALCRGEELRTG-FSARLSCRLEHRGEPYLFLMPAQLELVHDNPEILMFHNIASRSEIQEIKDLGNPLLTRSQVLGGKQGFNEVSSTRTSKTGWLDDSTNPTVSRLGRRIELLTGLKTNTSNDDAELLQVANYMNGGHYSPHFDYVMKDKAPDHMIYLPYSNQYIGDRLATWMFYLNEVGEGGRTVFPRLKAGVKPEAGAAVFWYNLVGSGDADHRTLHGACPVLYGTKW 435 HSP 2 Score: 189.889 bits (481), Expect = 4.958e-48 Identity = 107/240 (44.58%), Postives = 137/240 (57.08%), Query Frame = 0
Query: 1159 EDDI--LKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQV--GARWLGGVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGKRT----------------------LLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
EDD ALCRGEE RT + A+L C L H+ +PY L PA++E+ HD P IL+F NI S++E+ I+D+ L R+QV G + VS R S + W+ DS +P RL R+ +TGLKTN+ +DAE LQV NY+NGG Y H D+ K LN+VG GGRTVFP + AG+ PEAG+AVFWYNL S D T H ACP+ +GTKW
Sbjct: 200 EDDAANFAALCRGEELRTGFS-ARLSCRLEHRGEPYLFLMPAQLELVHDNPEILMFHNIASRSEIQEIKDLGNPLLTRSQVLGGKQGFNEVSSTRTSKTGWLDDSTNPTVSRLGRRIELLTGLKTNTS---NDDAELLQVANYMNGGHYSPHFDYVMKDKAPDHMIYLPYSNQYIGDRLATWMFYLNEVGEGGRTVFPRLKAGVKPEAGAAVFWYNLVGSGDADHRTLHGACPVLYGTKW 435 HSP 3 Score: 181.415 bits (459), Expect = 3.350e-45 Identity = 146/469 (31.13%), Postives = 219/469 (46.70%), Query Frame = 0
Query: 514 FELILLGCLLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLE-----WF----HQARILAEQEGNRTLDVTTAQKYLDLTAEF----HDEQFRSPWGLKQE----------VFPVP-----YYKDPN----------------------LVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPAR-IEIAHLQPQILVFRDVVTPNDMDLL----RDIAKPL------LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
F +I L L QGEVFSS +M +F LER+L+ + QK ++ L ++ GNPI +++ VKRL + + +E+ + D + + V+R R +V P+E+DL GAA+ L+R+QDVYE+ + L +G+IG + A LTAQDCL+IGKHC+N +LS S+E W+ Q + + + +R +V Q D A F E+ R+ + + +F +P + +P L+ + + L K+ + ++ T++ SKTG + P+ R L+ L TS ND LLQ A + H P P+ M L + I L LN VG GGRTVFP + AG++PEAG+AVFWYNL + D TLHGACP+++GTKW
Sbjct: 5 FTIIFLFGLQSTQGEVFSSVSDMSRVFQLERDLVGVLLDFAQKTQNKLNKI-------------------------AGNPIHTYNVVKRLAVDFVSLEKEISFDDWKENSLRVERLRLGGVV-PKEKDLHGAAQALIRLQDVYELEISHLVRGRIGDRDTTAKLTAQDCLFIGKHCFNGGILSRSIEWFEEAWYLAGEEQNTTVTQDKVSRNYEVVDRQGDEDDAANFAALCRGEELRTGFSARLSCRLEHRGEPYLFLMPAQLELVHDNPEILMFHNIASRSEIQEIKDLGNPLLTRSQVLGGKQGFNEVSSTRT--SKTGWLDDSTNPTVSRLG--------RRIELLTGLKTNTS--NDDAELLQVANYMNGGHYSPHFDYVMKDKAPDHMIYLPYSNQYIGDRLATWMFYLNEVGEGGRTVFPRLKAGVKPEAGAAVFWYNLVGSGDADHRTLHGACPVLYGTKW 435 HSP 4 Score: 85.1149 bits (209), Expect = 3.573e-13 Identity = 64/183 (34.97%), Postives = 92/183 (50.27%), Query Frame = 0
Query: 915 IMFG--TKWGEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHI--------NAYLKDFEEVVDME-----DNSNETQPVVPALYSVQRLASTYQHLEADLEEDLYEHFATLDVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTL 1082
+FG + GEV+SS M +VF LER+L+ ++ A+ + KL I N + + V +E D+ E V L RL E DL DVYE+ + L +G++G +D + A+L AQDCL+IGKHCFN +LS S+EWF++A LA E N T+
Sbjct: 9 FLFGLQSTQGEVFSSVSDMSRVFQLERDLVGVLLDFAQKTQNKLNKIAGNPIHTYNVVKRLAVDFVSLEKEISFDDWKENSLRVERL----RLGGVVPK-EKDLHGAAQALIRLQDVYELEISHLVRGRIGDRD----TTAKLTAQDCLFIGKHCFNGGILSRSIEWFEEAWYLAGEEQNTTV 182
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Match: gi|1022772068|gb|KZS16263.1| (Uncharacterized protein APZ42_017879 [Daphnia magna]) HSP 1 Score: 377.096 bits (967), Expect = 6.941e-113 Identity = 193/488 (39.55%), Postives = 291/488 (59.63%), Query Frame = 0
Query: 25 AEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVLKGERGPGL--------FPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PHKGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
+++S+++D+ F +E +V++L + + E+KL I Y+ +Y ++EEK + S+EEFME++ GNPIHAY+LMKR DW+ +E++++ D W + R+ + FP++ED G+AQ+L+RLQD YEL++ L DCLF+GKH FN G +R++EWFEE++ILAGQE N TL QDQV FI HA HD+ + + ++ + F P+ +P VQR ++ + +++T +QDD FNALCRGE L + +A LKC Y + PY+ L P++IE +P + FH+VLS +E+ I+ +A PLL RS VQG+ ++S+VRTSKT+WL + PL+ +T+R+ +TGLKTN + +E+ELLQ+ N PH + + F ++ V GG T FPR G +V+ G A FW NL +SG+ D TLHGACP++ G KW S
Sbjct: 25 GDMFSASADLANTFRLEQQVVNVLGELVAQTEAKLGIIRQYLKDYESVVEEKAA--SEEEFMERVAGNPIHAYRLMKRLYFDWQTVEKEIKTDNWRNALRQLEHVRMGAKFPKEEDFFGAAQALVRLQDTYELNMTQLARGNLWGRQTRAELTAQDCLFMGKHAFNAGAYARAVEWFEESYILAGQENNRTLRQDQVMEFIQHAINMHDDTLQQTKKRHPMAVLSHTNSFRLPLKEKPAGVQRSEIIRSRKYNVGKHIT-------DQDDATNFNALCRGETLLNDKLLAQLKCWYDSRRQPYFLLMPIKIEQNSMEPAIYSFHDVLSDDEIETIKQLAKPLLARSMVQGRTGVGHEVSNVRTSKTAWLPEGLHPLLNRLTRRISLITGLKTNPISDEAELLQVANYGIGGHYAPHHDYLMKGKAD---FELNDVERGGSTAFPRAGVAVKPVRGGAAFWFNLKRSGKPDPLTLHGACPVLLGHKWVS 500 HSP 2 Score: 246.128 bits (627), Expect = 8.745e-67 Identity = 160/492 (32.52%), Postives = 247/492 (50.20%), Query Frame = 0
Query: 922 GEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDMEDNSNE------TQPVVPALYSVQRLASTYQHLEADL-----------------------EEDLYEHFATL----DVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLFPTSYAANPNVFQ---QRRHANEEKSKATRIRAQAKMASHENWLPELSREDDI--LKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGG--VSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGKRTL-LNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
G+++S+S + F LE++++ ++ E KL I YLKD+E VV+ + S E + A ++RL +Q +E ++ EED + L D YE+ + +L++G + + + A L AQDCL++GKH FN + ++EWF+++ +LA +E N+TL +++ A N HD+ L + P + ++ N F+ + + A ++S+ R R + + H ++ +DD ALCRGE + LAQL C+ + PY+ L P K+E P I F +++S +E+ I+ +A+ L R+ V R G VS R S + W+ + HP+ RL R+S +TGLKTN ++AE LQV NY GG Y HHD+ GK LNDV GG T FP G + P G A FW+NLK+S PD LT H ACP+ G KW
Sbjct: 25 GDMFSASADLANTFRLEQQVVNVLGELVAQTEAKLGIIRQYLKDYESVVEEKAASEEEFMERVAGNPIHAYRLMKRLYFDWQTVEKEIKTDNWRNALRQLEHVRMGAKFPKEEDFFGAAQALVRLQDTYELNMTQLARGNLWGR----QTRAELTAQDCLFMGKHAFNAGAYARAVEWFEESYILAGQENNRTLRQDQVMEFIQHAINMHDDTLQ----QTKKRHPMAVLSHTNSFRLPLKEKPAGVQRSEIIRSR-KYNVGKH------ITDQDDATNFNALCRGETLLNDKLLAQLKCWYDSRRQPYFLLMPIKIEQNSMEPAIYSFHDVLSDDEIETIKQLAKPLLARSMVQGRTGVGHEVSNVRTSKTAWLPEGLHPLLNRLTRRISLITGLKTNPI---SDEAELLQVANYGIGGHYAPHHDYLMKGKADFELNDVERGGSTAFPRAGVAVKPVRGGAAFWFNLKRSGKPDPLTLHGACPVLLGHKW 498 HSP 3 Score: 170.629 bits (431), Expect = 5.021e-41 Identity = 106/374 (28.34%), Postives = 186/374 (49.73%), Query Frame = 0
Query: 513 VFELILLGCLLRVQ---GEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQF-----RSPWGLKQEV--FPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLLNNVGGGGRT 876
F + + LLR Q G++FS++ ++ F LE+ ++ + + + E L + YLK++ + GNPI ++ +KRL ++ VE+ + D + + ++ R P+EED GAA+ L+R+QD YE+ + L++G + ++A LTAQDCL++GKH +N + ++EWF ++ ILA QE NRTL +++ HD+ R P + F +P + P VQ+ + + +++ N K + A FNA+CRG L++L C+ + Y+LL P +IE ++P I F DV++ ++++ ++ +AKPLL GRT
Sbjct: 8 TFLITITALLLRNQPVVGDMFSASADLANTFRLEQQVVNVLGELVAQTEAKLGIIRQYLKDYESVVEEKAASEEEFMERVAGNPIHAYRLMKRLYFDWQTVEKEIKTDNWRNALRQLEH-VRMGAKFPKEEDFFGAAQALVRLQDTYELNMTQLARGNLWGRQTRAELTAQDCLFMGKHAFNAGAYARAVEWFEESYILAGQENNRTLRQDQVMEFIQHAINMHDDTLQQTKKRHPMAVLSHTNSFRLPLKEKPAGVQRSEIIRSRKY----NVGKHITDQD---------DATNFNALCRGETLLNDKLLAQLKCWYDSRRQPYFLLMPIKIEQNSMEPAIYSFHDVLSDDEIETIKQLAKPLLARSMVQGRT 367 HSP 4 Score: 74.7146 bits (182), Expect = 8.468e-10 Identity = 31/64 (48.44%), Postives = 41/64 (64.06%), Query Frame = 0
Query: 858 LLRDIAKPLLNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
L++ A LN+V GG T FP G ++P G A FW+NL ++ PDP TLHGACP++ G KW
Sbjct: 435 LMKGKADFELNDVERGGSTAFPRAGVAVKPVRGGAAFWFNLKRSGKPDPLTLHGACPVLLGHKW 498
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Match: gi|321463241|gb|EFX74258.1| (hypothetical protein DAPPUDRAFT_22132, partial [Daphnia pulex]) HSP 1 Score: 376.711 bits (966), Expect = 8.249e-113 Identity = 197/516 (38.18%), Postives = 303/516 (58.72%), Query Frame = 0
Query: 12 LILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAET-----QFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHA--AQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMIN-----------------------LPHKGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
L++ G++ +++S+++D+ F +E +V++L + + E+KL I +Y+ +Y ++EEK S S+EEF+E++ GNPIHAY+LMKR DW+ +E++++ D W + R R+ + FP++ED G+AQ+L+RLQD YEL++ L DCLF+GKH FN +R++EWFEE++ILAG E N T+ QDQV FI HA Q+ + + F P+ P +VQR ++ + ++++ +QDD FNALCRGE L + +A LKC Y + Y+ L P++IE +P + FH+VLS EE+ I+++A PLL RS VQGK ++S+VRTSKT+WL + PL+ +++R+ +TGLKT+ +++E+ELLQ+ N + H+ + GDRIAT+MFY++ V GG T FPR G +V+ G A FW NL +SG+ D TLHGACP++ G KW S
Sbjct: 2 LVILGTRP---VSGDMFSASADLANTFRLEQKVVNVLGELVAQTEAKLSIIRHYLEDYQSVVEEKAS--SEEEFIERVAGNPIHAYRLMKRLYFDWQTVEKEIKADNWRSIFAFIITLILERVRMGAKFPKEEDFFGAAQALVRLQDTYELNMTQLARGNLWGRQTRAELTAQDCLFMGKHAFNARAYARAVEWFEESYILAGLENNKTIRQDQVMEFIQHAINMQTKQGKPMAVLSHTNSFELPLRERPAVVQRSEIIRSRKYNVGKHIS-------DQDDASNFNALCRGERLLNDKLLAELKCWYDTRHQFYFLLMPIKIEQHSFEPAIYTFHDVLSDEEIETIKELAKPLLARSMVQGKLGVGHEVSNVRTSKTAWLPEGLHPLLNRLSRRIGLITGLKTDPIRDEAELLQVANYGIGGHYSPHHDYLMKDKADFEYMHHRELQAGDRIATFMFYLNDVERGGSTAFPRAGVAVKPVKGGAAFWFNLKRSGKPDPLTLHGACPVLLGHKWVS 505 HSP 2 Score: 220.32 bits (560), Expect = 7.941e-58 Identity = 155/526 (29.47%), Postives = 256/526 (48.67%), Query Frame = 0
Query: 915 IMFGTK--WGEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDMEDNSNETQPV-------VPALYSVQRLASTYQHLEADLEED----LYEHFATL----------------------------DVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLFPTSYAANPNVFQ---QRRHANEEKSKATRIRAQAKMASHENWLPELSREDDI--LKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGG--VSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHC---------------RAGKRT-----LLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
++ GT+ G+++S+S + F LE++++ ++ E KL I YL+D++ VV+ E S+E + + + A ++RL +Q +E +++ D ++ TL D YE+ + +L++G + + + A L AQDCL++GKH FN + + ++EWF+++ +LA E N+T+ +++ A N +Q P + ++ N F+ + R A ++S+ R R + + H +S +DD ALCRGE + LA+L C+ ++ Y+ L P K+E P I F +++S E+ I+++A+ L R+ V + G VS R S + W+ + HP+ RL+ R+ +TGLKT+ +++AE LQV NY GG Y HHD+ +AG R LNDV GG T FP G + P G A FW+NLK+S PD LT H ACP+ G KW
Sbjct: 3 VILGTRPVSGDMFSASADLANTFRLEQKVVNVLGELVAQTEAKLSIIRHYLEDYQSVVE-EKASSEEEFIERVAGNPIHAYRLMKRLYFDWQTVEKEIKADNWRSIFAFIITLILERVRMGAKFPKEEDFFGAAQALVRLQDTYELNMTQLARGNLWGR----QTRAELTAQDCLFMGKHAFNARAYARAVEWFEESYILAGLENNKTIRQDQVMEFIQHAINMQTKQGK----------PMAVLSHTNSFELPLRERPAVVQRSEIIRSR-KYNVGKH------ISDQDDASNFNALCRGERLLNDKLLAELKCWYDTRHQFYFLLMPIKIEQHSFEPAIYTFHDVLSDEEIETIKELAKPLLARSMVQGKLGVGHEVSNVRTSKTAWLPEGLHPLLNRLSRRIGLITGLKTDPI---RDEAELLQVANYGIGGHYSPHHDYLMKDKADFEYMHHRELQAGDRIATFMFYLNDVERGGSTAFPRAGVAVKPVKGGAAFWFNLKRSGKPDPLTLHGACPVLLGHKW 503 HSP 3 Score: 161.384 bits (407), Expect = 6.945e-38 Identity = 97/357 (27.17%), Postives = 183/357 (51.26%), Query Frame = 0
Query: 516 LILLGCLLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDD---FAKSVKRDR-RRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQ-EVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
L++LG V G++FS++ ++ F LE+ ++ + + + E L + YL+++ + GNPI ++ +KRL ++ VE+ + D F ++ +R R P+EED GAA+ L+R+QD YE+ + L++G + ++A LTAQDCL++GKH +N + + ++EWF ++ ILA E N+T+ +++ +Q + L F +P + P +VQ+ + + +++ ++S + A FNA+CRG L++L C+ ++ Y+LL P +IE +P I F DV++ +++ ++++AKPLL
Sbjct: 2 LVILGTR-PVSGDMFSASADLANTFRLEQKVVNVLGELVAQTEAKLSIIRHYLEDYQSVVEEKASSEEEFIERVAGNPIHAYRLMKRLYFDWQTVEKEIKADNWRSIFAFIITLILERVRMGAKFPKEEDFFGAAQALVRLQDTYELNMTQLARGNLWGRQTRAELTAQDCLFMGKHAFNARAYARAVEWFEESYILAGLENNKTIRQDQVMEFIQHAINMQTKQGKPMAVLSHTNSFELPLRERPAVVQRSEIIRSRKY-----NVGKHISDQDD--------ASNFNALCRGERLLNDKLLAELKCWYDTRHQFYFLLMPIKIEQHSFEPAIYTFHDVLSDEEIETIKELAKPLL 344 HSP 4 Score: 74.7146 bits (182), Expect = 9.128e-10 Identity = 29/55 (52.73%), Postives = 37/55 (67.27%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
LN+V GG T FP G ++P G A FW+NL ++ PDP TLHGACP++ G KW
Sbjct: 449 LNDVERGGSTAFPRAGVAVKPVKGGAAFWFNLKRSGKPDPLTLHGACPVLLGHKW 503
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Match: gi|1022772651|gb|KZS16808.1| (Uncharacterized protein APZ42_017385 [Daphnia magna]) HSP 1 Score: 365.155 bits (936), Expect = 1.862e-108 Identity = 193/526 (36.69%), Postives = 303/526 (57.60%), Query Frame = 0
Query: 4 ELLWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRD-----------------LDCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVLKGERGPGL------FPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGS-TKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH-----KG-------------MFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGS 480
++++VT +I + +++S+++D+ ++F +E + ++L + + E+KLE I Y+ EY + + +D +E FME++ GNPIHAY+LMKR DW+ IE++++ D W T + R FP++ED G+ +L+RLQD YEL+I + LDCLF+GKH F+ G +R++EW EE++ILAG E N T+ QD + I A HDE + + ++ + F P+ +P ++QR ++ + S + ++DD +FNALCRGE L S + LKC Y PY +L P++IE +P + FH+VL+ E+ I+++A PLL R++V KQAGS T +S+VRTSKT+WL+D P++ +++R+ +TGLK N+ +E+E+LQ+ N PH KG + GDRIAT+MFY++ V GG T FPR G +V+ G A FW+NL ++G+ D TLHGACP++ G KW S
Sbjct: 8 QIVYVTMLII--------QVSGDMHSASADLASIFQMEQQVNNVLSELVSQTETKLEIIRQYLKEYEQSVINETMDDKNE-FMERLAGNPIHAYRLMKRLYFDWQLIEKEIKTDNWQSTLIKLEEVRNNEKFPKEEDFLGATSALVRLQDTYELNITELVRGNLRGRQTHAELTALDCLFMGKHAFDEGAYARAVEWLEESYILAGLENNQTIRQDHIMEVIQQAIDKHDEALHEAQQRMAVLSDTKSFRLPLKEKPAVLQRNEM-----------IRSRKY---DKDDAAKFNALCRGESLLSEEILGQLKCWYETHKEPYLFLMPIKIEQNSIRPAIYTFHDVLNDLEIETIKELATPLLARAKVLAKQAGSKTTVSNVRTSKTAWLEDELHPMLHRISQRIALVTGLKVNSSFDEAEILQVANYGIGGHYSPHHDYLMKGKTDEELGKMNARELATGDRIATFMFYLNDVERGGSTAFPRAGVAVKPVKGGAAFWYNLKRNGQGDPDTLHGACPVLLGHKWVS 510 HSP 2 Score: 217.238 bits (552), Expect = 8.932e-57 Identity = 160/515 (31.07%), Postives = 242/515 (46.99%), Query Frame = 0
Query: 922 GEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVDME--DNSNETQPV-----VPALYSVQRLASTYQHLEADL-----------------------EEDLYEHFATL----DVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLFPTSYAANPNVFQQRRHANEEKSKATRIRAQAKMASHENWLPELSRE---DDILK--ALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGG---VSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGK----------RTL------------LNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
G+++S+S + +F +E+++ ++ E KL+ I YLK++E+ V E D+ NE + A ++RL +Q +E ++ EED + L D YE+ + EL +G + + + A L A DCL++GKH F+E + ++EW +++ +LA E NQT+ + + A + HDE L+ Q+R A +K+ R+ + K A + SR+ DD K ALCRGE + E L QL C+ +PY L P K+E RP I F ++++ E+ I+++A L RA+V A+ G VS R S + W+ D HP+ R++ R++ +TGLK NS ++AE LQV NY GG Y HHD+ GK R L LNDV GG T FP G + P G A FWYNLK++ D T H ACP+ G KW
Sbjct: 21 GDMHSASADLASIFQMEQQVNNVLSELVSQTETKLEIIRQYLKEYEQSVINETMDDKNEFMERLAGNPIHAYRLMKRLYFDWQLIEKEIKTDNWQSTLIKLEEVRNNEKFPKEEDFLGATSALVRLQDTYELNITELVRGNLRGR----QTHAELTALDCLFMGKHAFDEGAYARAVEWLEESYILAGLENNQTIRQDHIMEVIQQAIDKHDEALHEA--------------------QQRMAVLSDTKSFRLPLKEKPAVLQRNEMIRSRKYDKDDAAKFNALCRGESLLSEEILGQLKCWYETHKEPYLFLMPIKIEQNSIRPAIYTFHDVLNDLEIETIKELATPLLARAKVLAKQAGSKTTVSNVRTSKTAWLEDELHPMLHRISQRIALVTGLKVNSSF---DEAEILQVANYGIGGHYSPHHDYLMKGKTDEELGKMNARELATGDRIATFMFYLNDVERGGSTAFPRAGVAVKPVKGGAAFWYNLKRNGQGDPDTLHGACPVLLGHKW 508 HSP 3 Score: 146.362 bits (368), Expect = 6.551e-33 Identity = 93/353 (26.35%), Postives = 175/353 (49.58%), Query Frame = 0
Query: 522 LLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQAT-SHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQD-LQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLD----VTTAQKYLDLTAE-FHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
+++V G++ S++ ++ IF +E+ + + + + E L + YLKE+ Q+ + D + GNPI ++ +KRL ++ +E+ + D Q K + R + P+EED GA L+R+QD YE+ + +L +G + + A LTA DCL++GKH ++E + ++EW ++ ILA E N+T+ + Q+ +D E H+ Q R + F +P + P ++Q+ + + ++++ A FNA+CRG + L +L C+ + Y L P +IE ++P I F DV+ +++ ++++A PLL
Sbjct: 16 IIQVSGDMHSASADLASIFQMEQQVNNVLSELVSQTETKLEIIRQYLKEYEQSVINETMDDKNEFMERLAGNPIHAYRLMKRLYFDWQLIEKEIKTDNWQSTLIKL--EEVRNNEKFPKEEDFLGATSALVRLQDTYELNITELVRGNLRGRQTHAELTALDCLFMGKHAFDEGAYARAVEWLEESYILAGLENNQTIRQDHIMEVIQQAIDKHDEALHEAQQRMAVLSDTKSFRLPLKEKPAVLQRNEMIRSRKYDK--------------------DDAAKFNALCRGESLLSEEILGQLKCWYETHKEPYLFLMPIKIEQNSIRPAIYTFHDVLNDLEIETIKELATPLL 346 HSP 4 Score: 78.9518 bits (193), Expect = 4.151e-11 Identity = 31/55 (56.36%), Postives = 37/55 (67.27%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
LN+V GG T FP G ++P G A FWYNL +N DPDTLHGACP++ G KW
Sbjct: 454 LNDVERGGSTAFPRAGVAVKPVKGGAAFWYNLKRNGQGDPDTLHGACPVLLGHKW 508
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Match: gi|954527334|gb|KRZ51941.1| (Prolyl 4-hydroxylase subunit alpha-2, partial [Trichinella nativa]) HSP 1 Score: 316.62 bits (810), Expect = 9.730e-86 Identity = 266/958 (27.77%), Postives = 439/958 (45.82%), Query Frame = 0
Query: 1 MANELLWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVF--PRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHA--AQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDL-----HQRLIENVTSPELVVDNQDD--IPR---FNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PHKGMFI---------------------------------GDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGSLVAYVNKRNDTKGDS--------GAIYKNGHVRLVKMKSRVFELILLGCLLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIV-LPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIIL----------KGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYL-LQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
M N + +TT +L G+ + ++ EV++S +++ + + D+ I+D Y ++ E +LE++ +EY + + + +E + +I NP+ ++ ++K+ + +W+ +E+ ++ + + + SV P +ED+ G A L+R+QDVY+LD D+ DCL I + +N +L W EAW +E T+ + V +I A Q + E + L Q P K +DL R I+ P DD IP + LCR E A L C+Y + PY L P+++EV H KP + F V+S EE+A I+ +A PLL R+ V G + + R SK++WL+D+ ++K ++ R++ MT L TM E +ELLQ+ N PH M G+RIAT +FYIS AGG TVF +VE + A FW N+ + GE D ST H ACP++ GTKW + NK +G G + K + L+ + + L+ C++R EVF++ ++ + E N++ +E +I E +LR + LK Q + A+ L NP+++F +K+L + VE + D D + +++ R S+ P EEDL GAA+ LLR+QDVY++ +LS G+I L A C +G+ YN+K H + W +A E E ++D +YL + + L + + + P+ R N K + D L K VS+ + L + P F A+CRG T S+LYCY ++ D +L L P ++E+ H +P+I++FR V++ N++ +L+ +A P L
Sbjct: 30 MLNMIWPLTTATLLLGAITPF-SRGEVFTSMANIDMLLQMGEDVSRIIDNYVEEDERRLEQLKKLSAEY----KSHKVQVHGQESTDSVIVNPVESFAIVKQLADNWRYVEQLMKTNSAEKLIQNFTHHTHNSVVRPPSEEDVIGMAVGLMRIQDVYKLDTHDMAEGKIRGVLDGRKLTAYDCLEIARVAYNKQDFYHTLLWATEAWDRVQKEDEPTIDEATVLEYIAFAMFKQGNIEWAI---HYTTLIKQVDPNHPRASGNLKYYQDLLDPEGKPRKIDPKKLPPPTNRRPDDLSIPERDVYEGLCRSEYPIPDKDRAKLYCYYK-RNRPYLKLAPIKVEVMHWKPKIVYFRGVISDEEIAVIKQLASPLLKRATVHNADTGQLETASYRISKSAWLKDTEHEVVKRISDRIDMMTDL---TM-ETAELLQIANYGIGGHYDPHFDMSTRGESDPYEEGTGNRIATVLFYTNDPYSFESLNAGNRIATVLFYISQPEAGGGTVFTSHKITVEPSKYDAAFWFNVLQGGEPDMSTRHAACPVLAGTKWVA-----NKWIHERGQEFRRPFFSPGCLLKTPTMALLHIIIFI-TLMDWWCVMRSSAEVFTAMADVENLIHTEDNVVDVIEQYI---ESDLRRLQR-LKSLAQEYRESKEKALKEGADRLYNPVNAFLFIKKLTEEWNDVELLMKSDHADIYLQNITAMRDSSLAKFPTEEDLTGAAEALLRLQDVYKLDTHELSSGRIKGAKQGLELDANGCFELGRVAYNQKDYYHVILWMQEALNRVEHENPPSVDQAEILEYLAY-GMYQQGNVKRALQLTKRLQRI----KPD--HPRAEGNVKWYLDLL--AKEGVSRVTDHDLPPIVNARPNDQALPERKDFEALCRGEYLLTEKQRSRLYCY--YKRDTPFLNLAPIKVEVMHWKPKIVIFRQVISANEIAVLKTLAYPRL 953 HSP 2 Score: 216.083 bits (549), Expect = 3.307e-53 Identity = 152/524 (29.01%), Postives = 258/524 (49.24%), Query Frame = 0
Query: 5 LLWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDE---WAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDLD-----------------CLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAA--QSHDERVLK-GERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPEL--VVD---NQDDIPR---FNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
LL + + L C R+ AEV+++ +D++ + E ++VD++++Y + +L+R+ + EY E+ E +D + NP++A+ +K+ + +W +E ++ D + + ++R L + FP +EDL G+A++L+RLQDVY+LD +L C +G+ +N + W +EA E ++ Q ++ ++ + Q + +R L+ +R + P E + L L + + VT +L +V+ N +P F ALCRGE L + + L C+Y + P+ L P+++EV H KP + IF V+S E+A ++ +A P L R+ V K+ G + + R SK++WL++ P++ + KR+ MT L T +E LQ+ N PH K + G+RIAT +FYIS AGG TVF ++ G A FW NL +GE D ST H ACP++ G KW
Sbjct: 596 LLHIIIFITLMDWWCVMRSSAEVFTAMADVENLIHTEDNVVDVIEQYIESDLRRLQRLKSLAQEYRESKEKALKEGADRLY------NPVNAFLFIKKLTEEWNDVELLMKSDHADIYLQNITAMRDSSL-AKFPTEEDLTGAAEALLRLQDVYKLDTHELSSGRIKGAKQGLELDANGCFELGRVAYNQKDYYHVILWMQEALNRVEHENPPSVDQAEILEYLAYGMYQQGNVKRALQLTKRLQRIKPDHPRAEGNVKWYLDL---LAKEGVSRVTDHDLPPIVNARPNDQALPERKDFEALCRGEYLLTEKQRSRLYCYYK-RDTPFLNLAPIKVEVMHWKPKIVIFRQVISANEIAVLKTLAYPRLRRATVHNKETGQLEHASYRISKSAWLKEHEHPVVDRIVKRIHDMTNLNMET----AEDLQIANYGLGGHYDPHFDHARKEEVNAFKSLNTGNRIATVLFYISQPEAGGATVFITHKLAIFPVEGDAAFWFNLKPNGEGDMSTRHAACPVLAGVKW 1104 HSP 3 Score: 181.415 bits (459), Expect = 2.186e-42 Identity = 165/611 (27.00%), Postives = 262/611 (42.88%), Query Frame = 0
Query: 872 GGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW--------------------------------------------------GEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVD--MEDNSNETQPVVPALYSVQRLASTYQHLE-------ADL------------------EEDLYEHFATL----DVYEIGVPELSQGKM-GLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDE-------QLNNPWGHADQLFPTSYAANPNV-FQQRRHANEEKSKATR--IRAQAKMASHENWLPELSREDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGV--SKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCR-----------AGKR--TLLNDVG---GGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
GG TVF +EP A FW+N+ + PD T H ACP++ GTKW EV+++ ++ + E ++ +++ E+ +L+ + + +++ E + +++ ++ V A +++L + +E AD+ EEDL L DVY++ ELS G++ G K G LDA C +G+ +N+K + + W Q+A E E +++ + +YL A + + QL ++ P A NV + A E S+ T + ++ LPE R+D +ALCRGE T + ++L+CY ++ P+ L P KVE+ H +P+I++FR +IS NE+ V++ +A L+RA V + G + + RIS S W+ + HP+ R+ R+ MT L + E AE LQ+ NY GG Y H DH R G R T+L + GG TVF I P G A FW+NLK + D T HAACP+ G KW
Sbjct: 516 AGGGTVFTSHKITVEPSKYDAAFWFNVLQGGEPDMSTRHAACPVLAGTKWVANKWIHERGQEFRRPFFSPGCLLKTPTMALLHIIIFITLMDWWCVMRSSAEVFTAMADVENLIHTEDNVVDVIEQYIESDLRRLQRLKSLAQEYRESKEKALKEGADRLYNPVNAFLFIKKLTEEWNDVELLMKSDHADIYLQNITAMRDSSLAKFPTEEDLTGAAEALLRLQDVYKLDTHELSSGRIKGAK-----QGLELDANGCFELGRVAYNQKDYYHVILWMQEALNRVEHENPPSVDQAEILEYL--AYGMYQQGNVKRALQLTK---RLQRIKPDHPRAEGNVKWYLDLLAKEGVSRVTDHDLPPIVNARPNDQALPE--RKD--FEALCRGEYLLTEKQRSRLYCYYK-RDTPFLNLAPIKVEVMHWKPKIVIFRQVISANEIAVLKTLAYPRLRRATVHNKETGQLEHASYRISKSAWLKEHEHPVVDRIVKRIHDMTNL-------NMETAEDLQIANYGLGGHYDPHFDHARKEEVNAFKSLNTGNRIATVLFYISQPEAGGATVFITHKLAIFPVEGDAAFWFNLKPNGEGDMSTRHAACPVLAGVKW 1104 HSP 4 Score: 153.68 bits (387), Expect = 1.018e-33 Identity = 145/531 (27.31%), Postives = 241/531 (45.39%), Query Frame = 0
Query: 922 GEVYSSSGGMDQVFGLERELLQIMDSQAEALE---EKLKHINAYLKDFEEVVDMEDNSNET--QPVVPALYSVQRLASTYQHLEADLE----EDLYEHFA-------------------------TLDVYEIGVPELSQGKM-GLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWG-HADQLFPTSYAANPNVFQQRRHANE---EKSKATRIRAQAKMASHENWLPE-LS-REDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGV--SKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHD--------------------------------HCRAGKR--TLLNDVG---GGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
GEV++S +D + + ++ +I+D+ E E E+LK ++A K + V +++++ PV + V++LA ++++E ++ E L ++F DVY++ ++++GK+ G+ D G +L A DCL I + +N++ ++L W +A ++E T++ + +Y+ A +Q N W H L +P ++ + + K +I + K+ N P+ LS E D+ + LCR E + A+L+CY +N PY +L P KVE+ H +P+I+ FR +IS E+ VI+ +A L+RA V G + + RIS S W+ D+ H + +R++DR+ MT L E AE LQ+ NY GG Y H D AG R T+L + GG TVF + P A FW+N+ + PD T HAACP+ GTKW
Sbjct: 53 GEVFTSMANIDMLLQMGEDVSRIIDNYVEEDERRLEQLKKLSAEYKSHKVQVHGQESTDSVIVNPV-ESFAIVKQLADNWRYVEQLMKTNSAEKLIQNFTHHTHNSVVRPPSEEDVIGMAVGLMRIQDVYKLDTHDMAEGKIRGVLD-----GRKLTAYDCLEIARVAYNKQDFYHTLLWATEAWDRVQKEDEPTIDEATVLEYIAFAMF---KQGNIEWAIHYTTLIKQVDPNHPRASGNLKYYQDLLDPEGKPRKIDPK-KLPPPTNRRPDDLSIPERDVYEGLCRSEYPIPDKDRAKLYCYYK-RNRPYLKLAPIKVEVMHWKPKIVYFRGVISDEEIAVIKQLASPLLKRATVHNADTGQLETASYRISKSAWLKDTEHEVVKRISDRIDMMTDLTM-------ETAELLQIANYGIGGHYDPHFDMSTRGESDPYEEGTGNRIATVLFYTNDPYSFESLNAGNRIATVLFYISQPEAGGGTVFTSHKITVEPSKYDAAFWFNVLQGGEPDMSTRHAACPVLAGTKW 565
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Match: gi|954527339|gb|KRZ51946.1| (Prolyl 4-hydroxylase subunit alpha-2, partial [Trichinella nativa]) HSP 1 Score: 316.62 bits (810), Expect = 1.078e-85 Identity = 266/959 (27.74%), Postives = 440/959 (45.88%), Query Frame = 0
Query: 1 MANELLWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVF--PRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHA--AQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDL-----HQRLIENVTSPELVVDNQDD--IPR---FNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PHKGMFI---------------------------------GDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGSLVAYVNKRNDTKGDS--------GAIYKNGHVRLVKMKSRVFELILLGCLLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIV-LPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIIL----------KGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYL-LQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLLN 868
M N + +TT +L G+ + ++ EV++S +++ + + D+ I+D Y ++ E +LE++ +EY + + + +E + +I NP+ ++ ++K+ + +W+ +E+ ++ + + + SV P +ED+ G A L+R+QDVY+LD D+ DCL I + +N +L W EAW +E T+ + V +I A Q + E + L Q P K +DL R I+ P DD IP + LCR E A L C+Y + PY L P+++EV H KP + F V+S EE+A I+ +A PLL R+ V G + + R SK++WL+D+ ++K ++ R++ MT L TM E +ELLQ+ N PH M G+RIAT +FYIS AGG TVF +VE + A FW N+ + GE D ST H ACP++ GTKW + NK +G G + K + L+ + + L+ C++R EVF++ ++ + E N++ +E +I E +LR + LK Q + A+ L NP+++F +K+L + VE + D D + +++ R S+ P EEDL GAA+ LLR+QDVY++ +LS G+I L A C +G+ YN+K H + W +A E E ++D +YL + + L + + + P+ R N K + D L K VS+ + L + P F A+CRG T S+LYCY ++ D +L L P ++E+ H +P+I++FR V++ N++ +L+ +A P L+
Sbjct: 30 MLNMIWPLTTATLLLGAITPF-SRGEVFTSMANIDMLLQMGEDVSRIIDNYVEEDERRLEQLKKLSAEY----KSHKVQVHGQESTDSVIVNPVESFAIVKQLADNWRYVEQLMKTNSAEKLIQNFTHHTHNSVVRPPSEEDVIGMAVGLMRIQDVYKLDTHDMAEGKIRGVLDGRKLTAYDCLEIARVAYNKQDFYHTLLWATEAWDRVQKEDEPTIDEATVLEYIAFAMFKQGNIEWAI---HYTTLIKQVDPNHPRASGNLKYYQDLLDPEGKPRKIDPKKLPPPTNRRPDDLSIPERDVYEGLCRSEYPIPDKDRAKLYCYYK-RNRPYLKLAPIKVEVMHWKPKIVYFRGVISDEEIAVIKQLASPLLKRATVHNADTGQLETASYRISKSAWLKDTEHEVVKRISDRIDMMTDL---TM-ETAELLQIANYGIGGHYDPHFDMSTRGESDPYEEGTGNRIATVLFYTNDPYSFESLNAGNRIATVLFYISQPEAGGGTVFTSHKITVEPSKYDAAFWFNVLQGGEPDMSTRHAACPVLAGTKWVA-----NKWIHERGQEFRRPFFSPGCLLKTPTMALLHIIIFI-TLMDWWCVMRSSAEVFTAMADVENLIHTEDNVVDVIEQYI---ESDLRRLQR-LKSLAQEYRESKEKALKEGADRLYNPVNAFLFIKKLTEEWNDVELLMKSDHADIYLQNITAMRDSSLAKFPTEEDLTGAAEALLRLQDVYKLDTHELSSGRIKGAKQGLELDANGCFELGRVAYNQKDYYHVILWMQEALNRVEHENPPSVDQAEILEYLAY-GMYQQGNVKRALQLTKRLQRI----KPD--HPRAEGNVKWYLDLL--AKEGVSRVTDHDLPPIVNARPNDQALPERKDFEALCRGEYLLTEKQRSRLYCY--YKRDTPFLNLAPIKVEVMHWKPKIVIFRQVISANEIAVLKTLAYPRLS 954 HSP 2 Score: 216.853 bits (551), Expect = 1.827e-53 Identity = 152/524 (29.01%), Postives = 258/524 (49.24%), Query Frame = 0
Query: 5 LLWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDE---WAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDLD-----------------CLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAA--QSHDERVLK-GERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPEL--VVD---NQDDIPR---FNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
LL + + L C R+ AEV+++ +D++ + E ++VD++++Y + +L+R+ + EY E+ E +D + NP++A+ +K+ + +W +E ++ D + + ++R L + FP +EDL G+A++L+RLQDVY+LD +L C +G+ +N + W +EA E ++ Q ++ ++ + Q + +R L+ +R + P E + L L + + VT +L +V+ N +P F ALCRGE L + + L C+Y + P+ L P+++EV H KP + IF V+S E+A ++ +A P L R+ VQ + G + + R SK++WL++ P++ + KR+ MT L T +E LQ+ N PH K + G+RIAT +FYIS AGG TVF ++ G A FW NL +GE D ST H ACP++ G KW
Sbjct: 596 LLHIIIFITLMDWWCVMRSSAEVFTAMADVENLIHTEDNVVDVIEQYIESDLRRLQRLKSLAQEYRESKEKALKEGADRLY------NPVNAFLFIKKLTEEWNDVELLMKSDHADIYLQNITAMRDSSL-AKFPTEEDLTGAAEALLRLQDVYKLDTHELSSGRIKGAKQGLELDANGCFELGRVAYNQKDYYHVILWMQEALNRVEHENPPSVDQAEILEYLAYGMYQQGNVKRALQLTKRLQRIKPDHPRAEGNVKWYLDL---LAKEGVSRVTDHDLPPIVNARPNDQALPERKDFEALCRGEYLLTEKQRSRLYCYYK-RDTPFLNLAPIKVEVMHWKPKIVIFRQVISANEIAVLKTLAYPRLSRATVQNSETGELETAKYRISKSAWLKEHEHPVVDRIVKRIHDMTNLNMET----AEDLQIANYGLGGHYDPHFDHARKEEVNAFKSLNTGNRIATVLFYISQPEAGGATVFITHKLAIFPVEGDAAFWFNLKPNGEGDMSTRHAACPVLAGVKW 1104 HSP 3 Score: 182.185 bits (461), Expect = 1.576e-42 Identity = 166/611 (27.17%), Postives = 261/611 (42.72%), Query Frame = 0
Query: 872 GGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW--------------------------------------------------GEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVD--MEDNSNETQPVVPALYSVQRLASTYQHLE-------ADL------------------EEDLYEHFATL----DVYEIGVPELSQGKM-GLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDE-------QLNNPWGHADQLFPTSYAANPNV-FQQRRHANEEKSKATR--IRAQAKMASHENWLPELSREDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGV--SKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCR-----------AGKR--TLLNDVG---GGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
GG TVF +EP A FW+N+ + PD T H ACP++ GTKW EV+++ ++ + E ++ +++ E+ +L+ + + +++ E + +++ ++ V A +++L + +E AD+ EEDL L DVY++ ELS G++ G K G LDA C +G+ +N+K + + W Q+A E E +++ + +YL A + + QL ++ P A NV + A E S+ T + ++ LPE R+D +ALCRGE T + ++L+CY ++ P+ L P KVE+ H +P+I++FR +IS NE+ V++ +A L RA V G + +K RIS S W+ + HP+ R+ R+ MT L + E AE LQ+ NY GG Y H DH R G R T+L + GG TVF I P G A FW+NLK + D T HAACP+ G KW
Sbjct: 516 AGGGTVFTSHKITVEPSKYDAAFWFNVLQGGEPDMSTRHAACPVLAGTKWVANKWIHERGQEFRRPFFSPGCLLKTPTMALLHIIIFITLMDWWCVMRSSAEVFTAMADVENLIHTEDNVVDVIEQYIESDLRRLQRLKSLAQEYRESKEKALKEGADRLYNPVNAFLFIKKLTEEWNDVELLMKSDHADIYLQNITAMRDSSLAKFPTEEDLTGAAEALLRLQDVYKLDTHELSSGRIKGAK-----QGLELDANGCFELGRVAYNQKDYYHVILWMQEALNRVEHENPPSVDQAEILEYL--AYGMYQQGNVKRALQLTK---RLQRIKPDHPRAEGNVKWYLDLLAKEGVSRVTDHDLPPIVNARPNDQALPE--RKD--FEALCRGEYLLTEKQRSRLYCYYK-RDTPFLNLAPIKVEVMHWKPKIVIFRQVISANEIAVLKTLAYPRLSRATVQNSETGELETAKYRISKSAWLKEHEHPVVDRIVKRIHDMTNL-------NMETAEDLQIANYGLGGHYDPHFDHARKEEVNAFKSLNTGNRIATVLFYISQPEAGGATVFITHKLAIFPVEGDAAFWFNLKPNGEGDMSTRHAACPVLAGVKW 1104 HSP 4 Score: 153.68 bits (387), Expect = 8.732e-34 Identity = 145/531 (27.31%), Postives = 241/531 (45.39%), Query Frame = 0
Query: 922 GEVYSSSGGMDQVFGLERELLQIMDSQAEALE---EKLKHINAYLKDFEEVVDMEDNSNET--QPVVPALYSVQRLASTYQHLEADLE----EDLYEHFA-------------------------TLDVYEIGVPELSQGKM-GLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWG-HADQLFPTSYAANPNVFQQRRHANE---EKSKATRIRAQAKMASHENWLPE-LS-REDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGV--SKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHD--------------------------------HCRAGKR--TLLNDVG---GGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
GEV++S +D + + ++ +I+D+ E E E+LK ++A K + V +++++ PV + V++LA ++++E ++ E L ++F DVY++ ++++GK+ G+ D G +L A DCL I + +N++ ++L W +A ++E T++ + +Y+ A +Q N W H L +P ++ + + K +I + K+ N P+ LS E D+ + LCR E + A+L+CY +N PY +L P KVE+ H +P+I+ FR +IS E+ VI+ +A L+RA V G + + RIS S W+ D+ H + +R++DR+ MT L E AE LQ+ NY GG Y H D AG R T+L + GG TVF + P A FW+N+ + PD T HAACP+ GTKW
Sbjct: 53 GEVFTSMANIDMLLQMGEDVSRIIDNYVEEDERRLEQLKKLSAEYKSHKVQVHGQESTDSVIVNPV-ESFAIVKQLADNWRYVEQLMKTNSAEKLIQNFTHHTHNSVVRPPSEEDVIGMAVGLMRIQDVYKLDTHDMAEGKIRGVLD-----GRKLTAYDCLEIARVAYNKQDFYHTLLWATEAWDRVQKEDEPTIDEATVLEYIAFAMF---KQGNIEWAIHYTTLIKQVDPNHPRASGNLKYYQDLLDPEGKPRKIDPK-KLPPPTNRRPDDLSIPERDVYEGLCRSEYPIPDKDRAKLYCYYK-RNRPYLKLAPIKVEVMHWKPKIVYFRGVISDEEIAVIKQLASPLLKRATVHNADTGQLETASYRISKSAWLKDTEHEVVKRISDRIDMMTDLTM-------ETAELLQIANYGIGGHYDPHFDMSTRGESDPYEEGTGNRIATVLFYTNDPYSFESLNAGNRIATVLFYISQPEAGGGTVFTSHKITVEPSKYDAAFWFNVLQGGEPDMSTRHAACPVLAGTKW 565
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Match: gi|954363752|gb|KRY30536.1| (Prolyl 4-hydroxylase subunit alpha-2 [Trichinella spiralis]) HSP 1 Score: 312.768 bits (800), Expect = 1.560e-84 Identity = 267/966 (27.64%), Postives = 437/966 (45.24%), Query Frame = 0
Query: 1 MANELLWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVF--PRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHA--AQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDL-----HQRLIENVTSPELVVDNQDD--IPR---FNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PHKGMFI---------------------------------GDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKWGSLVAYVNKRNDTKGD-------SGAIYKNGHVRLVKMKSRVFELILL---------GCLLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIV-LPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIIL----------KGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYL-LQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
M N + +TT +L G+ + ++ EV++S +++ + + D+ I+D Y ++ E +LE++ +EY + + + +E + +I NP+ ++ ++K+ + W+ +E+ ++ + + + SV P +ED+ G A L+R+QDVY+LD D+ DCL I + +N +L W EAW +E T+ + V +I A Q + E + L Q P K +DL R I+ P DD IP + LCR E S A L C+Y + PY L P+++EV H KP + F V+S EE+A I+ +A PLL R+ V G + + R SK++WL+D+ ++K ++ R++ MT L TM E +ELLQ+ N PH M G+RIAT +FYIS AGG TVF +VE + A FW N+ + GE D ST H ACP++ GTKW + NK +G S + L+ +K+ L+ C +R EVF++ ++ + E N++ +E +I E +LR + LK Q + A+ L NP+++F +K+L + VE + D D + +++ R S+ P EEDL GAA+ LLR+QDVY++ +LS G+I L A C +G+ YN+K H + W +A E E ++D +YL + + L + + + P+ R N K + D L K VS+ + L + P F A+CRG T S+LYCY ++ D +L L P ++E+ H +P+I++FR V++ N++ +L+ +A P L
Sbjct: 1 MLNMIWPLTTATLLLGAITPF-SRGEVFTSMANIDMLLQMGEDVSRIIDNYVEEDERRLEQLKKLSAEY----KSHKVQVHGQEGTDNVIVNPVESFAIVKQLADSWRYVEQLMKTNSAEKLIQNFTHHTHNSVVRPPSEEDVIGMAVGLMRIQDVYKLDTHDMAEGKIRGVLDGRKLTAYDCLEIARVAYNKQDFYHTLLWATEAWDRVQKEDEPTIDEATVLEYIAFAMFKQGNIEWAI---HYTTLIKQVDPNHPRASGNLKYYQDLLDPEGKPRKIDPKKLPPPTNRRPDDLSIPERDVYEGLCRSEYPISDKDRAKLYCYYK-RNRPYLKLAPIKVEVMHWKPKIVYFRGVISDEEIAVIKQLASPLLKRATVHNADTGQLETASYRISKSAWLKDTEHEVVKRISDRIDMMTDL---TM-ETAELLQIANYGIGGHYDPHFDMSTRGESDPYEEGTGNRIATVLFYTNDPYSFESLNAGNRIATVLFYISQPEAGGGTVFTSHKITVEPSKYDAAFWFNVLQGGEPDMSTRHAACPVLAGTKWVA-----NKWIHERGQEFRRPFFSPGCVTSATRLLLLLKAPTMALLHFIIFITLMDWWCAMRSSAEVFTAMADVENLIHTEDNVVDVIEQYI---ESDLRRLQR-LKSLAQEYRESKEKALKEGADRLYNPVNAFLFIKKLTEEWNDVELLMKSDHADIYLQNITAMRDSSLAKFPTEEDLTGAAEALLRLQDVYKLDTHELSSGRIKGAKQGLELDANGCFELGRVAYNQKDYYHVILWMQEALNRVEHENPPSVDQAEILEYLAY-GMYQQGNVKRALQLTKRLQRI----KPD--HPRAEGNVKWYLDLL--AKEGVSRVTDHDLPPIVNARPNDQALPERKDFEALCRGEYLLTEKQRSRLYCY--YKRDTPFLSLAPIKVEVMHWKPKIVIFRQVISANEIAVLKTLAYPRL 933 HSP 2 Score: 215.698 bits (548), Expect = 4.768e-53 Identity = 152/524 (29.01%), Postives = 258/524 (49.24%), Query Frame = 0
Query: 5 LLWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDE---WAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDLD-----------------CLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAA--QSHDERVLK-GERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPEL--VVD---NQDDIPR---FNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
LL + L C+ R+ AEV+++ +D++ + E ++VD++++Y + +L+R+ + EY E+ E +D + NP++A+ +K+ + +W +E ++ D + + ++R L + FP +EDL G+A++L+RLQDVY+LD +L C +G+ +N + W +EA E ++ Q ++ ++ + Q + +R L+ +R + P E + L L + + VT +L +V+ N +P F ALCRGE L + + L C+Y + P+ L P+++EV H KP + IF V+S E+A ++ +A P L R+ V K+ G + + R SK++WL++ P++ + KR+ MT L T +E LQ+ N PH K + G+RIAT +FYIS AGG TVF ++ G A FW NL +GE D ST H ACP++ G KW
Sbjct: 576 LLHFIIFITLMDWWCAMRSSAEVFTAMADVENLIHTEDNVVDVIEQYIESDLRRLQRLKSLAQEYRESKEKALKEGADRLY------NPVNAFLFIKKLTEEWNDVELLMKSDHADIYLQNITAMRDSSL-AKFPTEEDLTGAAEALLRLQDVYKLDTHELSSGRIKGAKQGLELDANGCFELGRVAYNQKDYYHVILWMQEALNRVEHENPPSVDQAEILEYLAYGMYQQGNVKRALQLTKRLQRIKPDHPRAEGNVKWYLDL---LAKEGVSRVTDHDLPPIVNARPNDQALPERKDFEALCRGEYLLTEKQRSRLYCYYK-RDTPFLSLAPIKVEVMHWKPKIVIFRQVISANEIAVLKTLAYPRLRRATVHNKETGQLEHASYRISKSAWLKEHEHPVVDRIVKRIHDMTNLNMET----AEDLQIANYGLGGHYDPHFDHARKEEVNAFKSLNTGNRIATVLFYISQPEAGGATVFITHKLAIFPVEGDAAFWFNLKPNGEGDMSTRHAACPVLAGVKW 1084 HSP 3 Score: 156.762 bits (395), Expect = 1.146e-34 Identity = 143/510 (28.04%), Postives = 234/510 (45.88%), Query Frame = 0
Query: 922 GEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDFEEVVD--MEDNSNETQPVVPALYSVQRLASTYQHLE-------ADL------------------EEDLYEHFATL----DVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDE-------QLNNPWGHADQLFPTSYAANPNV-FQQRRHANEEKSKATR--IRAQAKMASHENWLPELSREDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGV--SKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCR-----------AGKR--TLLNDVG---GGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
EV+++ ++ + E ++ +++ E+ +L+ + + +++ E + +++ ++ V A +++L + +E AD+ EEDL L DVY++ ELS G+ +K A G LDA C +G+ +N+K + + W Q+A E E +++ + +YL A + + QL ++ P A NV + A E S+ T + ++ LPE R+D +ALCRGE T + ++L+CY ++ P+ L P KVE+ H +P+I++FR +IS NE+ V++ +A L+RA V + G + + RIS S W+ + HP+ R+ R+ MT L + E AE LQ+ NY GG Y H DH R G R T+L + GG TVF I P G A FW+NLK + D T HAACP+ G KW
Sbjct: 596 AEVFTAMADVENLIHTEDNVVDVIEQYIESDLRRLQRLKSLAQEYRESKEKALKEGADRLYNPVNAFLFIKKLTEEWNDVELLMKSDHADIYLQNITAMRDSSLAKFPTEEDLTGAAEALLRLQDVYKLDTHELSSGR--IKGA--KQGLELDANGCFELGRVAYNQKDYYHVILWMQEALNRVEHENPPSVDQAEILEYL--AYGMYQQGNVKRALQLTK---RLQRIKPDHPRAEGNVKWYLDLLAKEGVSRVTDHDLPPIVNARPNDQALPE--RKD--FEALCRGEYLLTEKQRSRLYCYYK-RDTPFLSLAPIKVEVMHWKPKIVIFRQVISANEIAVLKTLAYPRLRRATVHNKETGQLEHASYRISKSAWLKEHEHPVVDRIVKRIHDMTNL-------NMETAEDLQIANYGLGGHYDPHFDHARKEEVNAFKSLNTGNRIATVLFYISQPEAGGATVFITHKLAIFPVEGDAAFWFNLKPNGEGDMSTRHAACPVLAGVKW 1084 HSP 4 Score: 156.377 bits (394), Expect = 1.196e-34 Identity = 146/530 (27.55%), Postives = 240/530 (45.28%), Query Frame = 0
Query: 922 GEVYSSSGGMDQVFGLERELLQIMDSQAEALE---EKLKHINAYLKDFE-EVVDMEDNSNETQPVVPALYSVQRLASTYQHLEADLE----EDLYEHFA-------------------------TLDVYEIGVPELSQGKM-GLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWG-HADQLFPTSYAANPNVFQQRRHANE---EKSKATRIRAQAKMASHENWLPE-LS-REDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGARWLGGV--SKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHD--------------------------------HCRAGKR--TLLNDVG---GGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
GEV++S +D + + ++ +I+D+ E E E+LK ++A K + +V E N V + V++LA +++++E ++ E L ++F DVY++ ++++GK+ G+ D G +L A DCL I + +N++ ++L W +A ++E T++ + +Y+ A +Q N W H L +P ++ + + K +I + K+ N P+ LS E D+ + LCR E + + A+L+CY +N PY +L P KVE+ H +P+I+ FR +IS E+ VI+ +A L+RA V G + + RIS S W+ D+ H + +R++DR+ MT L E AE LQ+ NY GG Y H D AG R T+L + GG TVF + P A FW+N+ + PD T HAACP+ GTKW
Sbjct: 24 GEVFTSMANIDMLLQMGEDVSRIIDNYVEEDERRLEQLKKLSAEYKSHKVQVHGQEGTDNVIVNPVESFAIVKQLADSWRYVEQLMKTNSAEKLIQNFTHHTHNSVVRPPSEEDVIGMAVGLMRIQDVYKLDTHDMAEGKIRGVLD-----GRKLTAYDCLEIARVAYNKQDFYHTLLWATEAWDRVQKEDEPTIDEATVLEYIAFAMF---KQGNIEWAIHYTTLIKQVDPNHPRASGNLKYYQDLLDPEGKPRKIDPK-KLPPPTNRRPDDLSIPERDVYEGLCRSEYPISDKDRAKLYCYYK-RNRPYLKLAPIKVEVMHWKPKIVYFRGVISDEEIAVIKQLASPLLKRATVHNADTGQLETASYRISKSAWLKDTEHEVVKRISDRIDMMTDLTM-------ETAELLQIANYGIGGHYDPHFDMSTRGESDPYEEGTGNRIATVLFYTNDPYSFESLNAGNRIATVLFYISQPEAGGGTVFTSHKITVEPSKYDAAFWFNVLQGGEPDMSTRHAACPVLAGTKW 536
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Match: gi|1238862986|ref|XP_022247737.1| (prolyl 4-hydroxylase subunit alpha-1-like [Limulus polyphemus]) HSP 1 Score: 288.115 bits (736), Expect = 2.161e-81 Identity = 172/499 (34.47%), Postives = 270/499 (54.11%), Query Frame = 0
Query: 9 TTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMSEYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRRKRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLIENVTSPELVVDNQD----DIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PHKG-MFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
T LI GS R QA++++S +++ V +E + +LD K +E KL+ + NY+ EY L E + + +++ E I NP+H + ++KR +V+W I + + ++W + + + + PRDEDL G+A +L+RLQ Y L+ D+ DCL++GKH FNNG ++EW EEA A E N T+S +++ F+D A + HD+ VL+G F Y + R +RL N+ S E + D D + ALCRGE LR+ L+C Y+ +G+PY L PV++E P + F++++++ E I++IA P+L RS+VQG++ ++S VRTSKT+WL+ P++ + +RV+++TGL T+ E +Q N PH ++IG ++ V GG TVFP++GA V GSAVFW+NL K+G D TLHGACP++ G KW
Sbjct: 6 VTILIFVGSVA--RMQADLFASTENLQHVLLLERQMAGVLDGVLKTMELKLDYLRNYLKEYYRLSHEIANTKNQDDYSE-FIENPLHVFHMIKRLAVEWSGIRQMMLENDWDRVEEIIHSEE--DLLPRDEDLQGAALALVRLQYTYNLNTTDMAQGRLSGINSISGLTARDCLYLGKHAFNNGYYGHAIEWLEEAISRANLERNQTVSVEEITLFLDIAIRVHDD-VLEG----SYFNNHAYETLGHLVRDTGDT---KRLRRNL-STERKLRRTDTSFFDYENYVALCRGERLRTAREEVHLRCRYTSRGHPYLILQPVKVEEQSLDPYIVQFYDLMTSREADTIQEIATPMLTRSRVQGQKNLEDEVSTVRTSKTAWLKPEDHPIVDRINRRVQYVTGLSTDVQNYHCETVQTTNYGVGGHYEPHYDFLYIGKTEEQ----LTDVTRGGATVFPKVGAGVWPLKGSAVFWYNLKKNGTEDNLTLHGACPVLHGRKW 486 HSP 2 Score: 217.238 bits (552), Expect = 6.127e-57 Identity = 153/488 (31.35%), Postives = 240/488 (49.18%), Query Frame = 0
Query: 922 GEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDF----EEVVDMEDNSNETQPV---VPALYSVQRLASTYQ------------------HLEADL---EEDLYEHFATL----DVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPW--GHADQLFPTSYAANPNVFQQRRHANEEKSKATRIRAQAKMASHENWLPELSREDDILKALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQV-GARWLGG-VSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHCRAGK-RTLLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
++++S+ + V LER++ ++D + +E KL ++ YLK++ E+ + ++ + ++ + + + ++RLA + H E DL +EDL L Y + +++QG++ + +NS + L A+DCLY+GKH FN +++EW ++A A E NQT++V +LD+A HD+ L + HA + + + RR+ + E+ R +EN++ ALCRGE RT L C + + PY L P KVE P I+ F ++++ E + IQ++A L R++V G + L VS R S + W+ HPI R+ RV ++TGL T+ + E +Q NY GG Y H+D GK L DV GG TVFP VGAG+ P GSAVFWYNLKK+ D+LT H ACP+ G KW
Sbjct: 20 ADLFASTENLQHVLLLERQMAGVLDGVLKTMELKLDYLRNYLKEYYRLSHEIANTKNQDDYSEFIENPLHVFHMIKRLAVEWSGIRQMMLENDWDRVEEIIHSEEDLLPRDEDLQGAALALVRLQYTYNLNTTDMAQGRL----SGINSISGLTARDCLYLGKHAFNNGYYGHAIEWLEEAISRANLERNQTVSVEEITLFLDIAIRVHDDVLEGSYFNNHAYETLGHLVRDTGDTKRLRRNLSTERKLR---RTDTSFFDYENYV-----------ALCRGERLRTAREEVHLRCRYTSRGHPYLILQPVKVEEQSLDPYIVQFYDLMTSREADTIQEIATPMLTRSRVQGQKNLEDEVSTVRTSKTAWLKPEDHPIVDRINRRVQYVTGLSTD---VQNYHCETVQTTNYGVGGHYEPHYDFLYIGKTEEQLTDVTRGGATVFPKVGAGVWPLKGSAVFWYNLKKNGTEDNLTLHGACPVLHGRKW 486 HSP 3 Score: 166.007 bits (419), Expect = 1.239e-39 Identity = 103/354 (29.10%), Postives = 182/354 (51.41%), Query Frame = 0
Query: 516 LILLGCLLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPSDLD--LGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
LI +G + R+Q ++F+S N++ + LER + ++ ++ +E L + YLKE+++ + I + D + NP+ FH +KRL + + + + + L++D+ + + +LP +EDLQGAA L+R+Q Y + D++QG++ + S + LTA+DCLY+GKH +N H++EW +A A E N+T+ V +LD+ HD+ + Y +LV R T + KR L+ T+ + +T + + A+CRG RT L C + + Y +LQP ++E L P I+ F D++T + D +++IA P+L
Sbjct: 9 LIFVGSVARMQADLFASTENLQHVLLLERQMAGVLDGVLKTMELKLDYLRNYLKEYYRLSHEIANTK-NQDDYSEFIENPLHVFHMIKRLAVEWSGIRQMM---LENDWDRVEEIIHSEEDLLPRDEDLQGAALALVRLQYTYNLNTTDMAQGRLSGINSISGLTARDCLYLGKHAFNNGYYGHAIEWLEEAISRANLERNQTVSVEEITLFLDIAIRVHDDVLEGSYFNNH-----AYETLGHLV--RDTGDTKRLRRNLS-TERKLRRTDTSFF----DYENYVALCRGERLRTAREEVHLRCRYTSRGHPYLILQPVKVEEQSLDPYIVQFYDLMTSREADTIQEIATPML 346 HSP 4 Score: 80.8777 bits (198), Expect = 1.006e-11 Identity = 35/55 (63.64%), Postives = 40/55 (72.73%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
L +V GG TVFP +GAG+ P GSAVFWYNL KN T D TLHGACP++ G KW
Sbjct: 432 LTDVTRGGATVFPKVGAGVWPLKGSAVFWYNLKKNGTEDNLTLHGACPVLHGRKW 486
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Match: gi|675372330|gb|KFM65232.1| (Prolyl 4-hydroxylase subunit alpha-2, partial [Stegodyphus mimosarum]) HSP 1 Score: 275.018 bits (702), Expect = 1.360e-76 Identity = 175/514 (34.05%), Postives = 265/514 (51.56%), Query Frame = 0
Query: 23 AQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMS----EYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRR--KRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHDERVLKGERGPGLFPQPVYVEPPLVQRQKLQEDLHQRLI----------ENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINL-------PH------------------KGMFIGDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
AQA+++SS + ++ + +E LV L Y + E KL ++ Y++ Y ++E+K SD E M + NP+ AY+L+KR +VDW I+ + D+W F VR+ + R + P+ +DL G+A +L+RLQD Y+L++ D+ D L++GK FN G + ++ W EEA A EGN T S ++ F A + HD LF P + L LH L ++ P++ V D+ + ALCRGE LRS + + L C + +PY L P++IE KP + +FH++L+ + +R IA P L RS VQ + GS +S RTS+ +W+ + PL+ + + VE +TGL T+ ++ +E++Q+ N PH K + GDR AT MFY+S V GG TVFP++GASV GSAVFW+NL +SG+ D TLHGACP+ G+KW
Sbjct: 14 AQADIFSSTAHLQNLLHLERHLVTTLHNYIDRTEMKLSQVKRYLNLFYDSYQDVLEDKVPVPSDGEDM---VANPLQAYQLVKRLAVDWNFIKSAMSTDDW----FPVRKLIRDYRQLMPKKDDLQGAALALVRLQDTYKLNMTDVARGDVLGVPQGTTLTARDLLYLGKQSFNAGYYAHAIRWLEEALSQAHLEGNRTASVAEINAFYQSAIEMHDR----------LFELP---DINLSDYDTFGYPLHGNLSLHRAAVATGHQSELGPDISV--HDEQANYMALCRGERLRSTAEESKLFCRTETRNHPYLLLRPLRIEERSLKPYVVVFHDLLTENQTEILRLIAEPKLARSMVQASK-GSEIVSVSRTSQNAWVGPTDHPLMANVYRLVEAVTGLSTDVEQDHAEIVQIANYGMGGHYTPHFDYLLVDKDPEELQYVHPKELDAGDRTATLMFYLSDVTRGGATVFPKVGASVWPKRGSAVFWYNLKQSGKEDALTLHGACPVAIGSKW 504 HSP 2 Score: 189.889 bits (481), Expect = 1.861e-47 Identity = 147/525 (28.00%), Postives = 243/525 (46.29%), Query Frame = 0
Query: 909 LHGACPIMFGTKWGEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKLKHINAYLKDF----EEVV-----------DMEDNSNETQPVVPAL----------------YSVQRLASTYQHL---EADLEEDLYEHFATLDVYEIGVPELSQGK-MGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLFPTSYAANPNVFQQRRHANEEKSKATRIRAQAKMASHENWL-PELSREDDILK--ALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGA-RWLGGVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHC-----------------RAGKRT-----LLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
L+ ++ T +++SS+ + + LER L+ + + + E KL + YL F ++V+ DM N + +V L + V++L Y+ L + DL+ D Y++ + ++++G +G+ G L A+D LY+GK FN ++++ W ++A A EGN+T +V+ + A HD P ++ + F H N +A A H++ L P++S D+ ALCRGE R+ ++L C +N PY L P ++E +P ++VF +++++N+ +++ +A L R+ V A + VS R S + W+ + HP+ + V +TGL T+ ++++ AE +Q+ NY GG Y H D+ AG RT L+DV GG TVFP VGA + P+ GSAVFWYNLK+S D LT H ACP+A G+KW
Sbjct: 3 LYVVATLLVTTAQADIFSSTAHLQNLLHLERHLVTTLHNYIDRTEMKLSQVKRYLNLFYDSYQDVLEDKVPVPSDGEDMVANPLQAYQLVKRLAVDWNFIKSAMSTDDWFPVRKLIRDYRQLMPKKDDLQGAALALVRLQDTYKLNMTDVARGDVLGVP-----QGTTLTARDLLYLGKQSFNAGYYAHAIRWLEEALSQAHLEGNRTASVAEINAFYQSAIEMHDRLFE---------LPDINLSDYDTFGYPLHGNLSLHRA------AVATGHQSELGPDISVHDEQANYMALCRGERLRSTAEESKLFCRTETRNHPYLLLRPLRIEERSLKPYVVVFHDLLTENQTEILRLIAEPKLARSMVQASKGSEIVSVSRTSQNAWVGPTDHPLMANVYRLVEAVTGLSTD---VEQDHAEIVQIANYGMGGHYTPHFDYLLVDKDPEELQYVHPKELDAGDRTATLMFYLSDVTRGGATVFPKVGASVWPKRGSAVFWYNLKQSGKEDALTLHGACPVAIGSKW 504 HSP 3 Score: 171.785 bits (434), Expect = 2.062e-41 Identity = 110/346 (31.79%), Postives = 183/346 (52.89%), Query Frame = 0
Query: 526 QGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHI-PDMAVGPSDLD--LGNPISSFHTVKRLGIFYKKVEESLDQDLQDDF-AKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVSKTGEIILKGAPSADVFNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
Q ++FSS +++ + LER+L+ T+ ++I + E L +V YL F+ + + D PSD + + NP+ ++ VKRL + + ++ ++ D D F + + RD R+ ++P+++DLQGAA L+R+QD Y++ + D+++G + + +LTA+D LY+GK +N +H++ W +A A EGNRT V + E HD F P + Y NL R + + S+ G I A+ + A+CRG R+ A SKL+C T +N Y LL+P RIE L+P ++VF D++T N ++LR IA+P L
Sbjct: 15 QADIFSSTAHLQNLLHLERHLVTTLHNYIDRTEMKLSQVKRYLNLFYDSYQDVLEDKVPVPSDGEDMVANPLQAYQLVKRLAVDWNFIKSAMSTD--DWFPVRKLIRDYRQ--LMPKKDDLQGAALALVRLQDTYKLNMTDVARGDVLGVPQGTTLTARDLLYLGKQSFNAGYYAHAIRWLEEALSQAHLEGNRTASVAEINAFYQSAIEMHDRLFELPDINLSDYDTFGYPLHGNLSLHRAAV-----------ATGHQSELGPDISVHDEQAN-YMALCRGERLRSTAEESKLFCRTETRNHPYLLLRPLRIEERSLKPYVVVFHDLLTENQTEILRLIAEPKL 344 HSP 4 Score: 74.3294 bits (181), Expect = 9.893e-10 Identity = 31/55 (56.36%), Postives = 40/55 (72.73%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
L++V GG TVFP +GA + P+ GSAVFWYNL ++ D TLHGACP+ G+KW
Sbjct: 450 LSDVTRGGATVFPKVGASVWPKRGSAVFWYNLKQSGKEDALTLHGACPVAIGSKW 504
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Match: gi|1009575424|ref|XP_015919452.1| (prolyl 4-hydroxylase subunit alpha-1 [Parasteatoda tepidariorum]) HSP 1 Score: 268.47 bits (685), Expect = 3.000e-74 Identity = 174/533 (32.65%), Postives = 268/533 (50.28%), Query Frame = 0
Query: 1 MANELLWVTTCLILFGSKCSWRAQAEVYSSASDMKTVFGIETDLVDILDKYAKKLESKLERINNYMS----EYNGLMEEKRSEDSDEEFMEKIIGNPIHAYKLMKRFSVDWKKIEEDLQGDEWAETQFSVRR--KRLRSVFPRDEDLNGSAQSLIRLQDVYELDIRDL-----------------DCLFIGKHCFNNGVISRSLEWFEEAWILAGQEGNATLSQDQVQTFIDHAAQSHD---ERVLKGERGPGLFPQPVYVEPPLVQ----RQKLQEDLHQRLIENVTSPELVVDNQDDIPRFNALCRGEELRSGSYVATLKCHYSHQGNPYYYLHPVQIEVAHHKPLLRIFHNVLSTEEMAKIRDIAGPLLMRSQVQGKQAGSTKISDVRTSKTSWLQDSFDPLIKAMTKRVEWMTGLKTNTMKEESELLQMINLPHKGMFI-------------------------GDRIATWMFYISHVPAGGRTVFPRLGASVEATGGSAVFWHNLHKSGEADKSTLHGACPIMFGTKW 478
M N + +IL + QA+++SS + ++ + +E LV L +Y ++ E+KL + Y++ Y ++EEK S E+ M +GNP+ A++L++R +VDW + +Q D+W VR+ + + P +DL G+A +LIRLQD Y+L++ ++ D L++GK F G + +++W EEA A QEGN T +V F A HD ER F P+ + V RQK + P++ V D+ + ALCRGE LR+ S + L C +Q +PY+ L P++IE P + FHN+L+ ++ ++ IA P L RS VQ + GS +S RTS+ +W+ PL+ + + VE +TGL T+ + +E++Q+ N G + GDR AT MFY+S V GG TVFP++GASV GSAVFW+NL K+G+ D TLHGACP+ G+KW
Sbjct: 1 MVNRMRQFIVTMILVST-----VQADIFSSTAHLQNLLHLERHLVTTLHEYVERTETKLNEVRRYLNLFYESYQEVLEEKVPVSSGEDEM---VGNPLQAFQLIRRLAVDWGFVRNAMQSDDWN----PVRKLTNDYKQLMPSRDDLQGAALALIRLQDTYKLNMTEVAKGEILGRAQGTILTARDLLYLGKESFTAGYYAHAIQWLEEALTKAHQEGNRTAGVSEVMAFYTSAVDMHDRLFERTDIDVADYDTFGHPLKGQHSRVAIAAGRQKPK-----------LGPDISV--HDEQANYMALCRGERLRTHSEESKLTCRLDNQRHPYFLLQPLRIEERGLNPYVVQFHNLLTESQVDLLKIIAEPKLARSMVQAGK-GSEVVSVSRTSQNAWVGPHDHPLMAKVYQLVETVTGLSTDVDNKHAEIVQIANYGMGGHYTPHFDYLLVDKEPEELQYVHPHELESGDRTATLMFYLSDVTRGGATVFPKIGASVWPEKGSAVFWYNLKKNGKEDPMTLHGACPVAVGSKW 507 HSP 2 Score: 192.971 bits (489), Expect = 1.570e-48 Identity = 145/519 (27.94%), Postives = 247/519 (47.59%), Query Frame = 0
Query: 915 IMFGTKWGEVYSSSGGMDQVFGLERELLQIMDSQAEALEEKL----KHINAYLKDFEEVVD--MEDNSNETQPV---VPALYSVQRLASTYQHLEADLEEDLYEHFATL-------------------------DVYEIGVPELSQGKMGLKDAILNSGARLDAQDCLYIGKHCFNEKLLSNSLEWFQQARVLAEREGNQTLNVSVAQKYLDLASNFHDEQLNNPWGHADQLFPTSY--AANPNVFQQRRHANEEKSKATRIRAQAKMASHENWLPELSREDDILK--ALCRGEEFRTPETLAQLHCYLSHQNDPYYRLHPAKVEIAHDRPRILVFRNIISQNEMNVIQDMARHTLQRAQVGA-RWLGGVSKDRISTSTWISDSHHPIGRRLADRVSHMTGLKTNSDLLDKEDAEHLQVGNYINGGLYHIHHDHC-----------------RAGKRT-----LLNDVGGGGRTVFPLVGAGISPEAGSAVFWYNLKKSLMPDDLTAHAACPLAFGTKW 1372
I+ T +++SS+ + + LER L+ + E E KL +++N + + ++EV++ + +S E + V + A ++RLA + + ++ D + L D Y++ + E+++G++ L A G L A+D LY+GK F +++++W ++A A +EGN+T VS + A + HD +LF + A+ + F + R + K+ P++S D+ ALCRGE RT ++L C L +Q PY+ L P ++E P ++ F N+++++++++++ +A L R+ V A + VS R S + W+ HP+ ++ V +TGL T+ +D + AE +Q+ NY GG Y H D+ +G RT L+DV GG TVFP +GA + PE GSAVFWYNLKK+ D +T H ACP+A G+KW
Sbjct: 13 ILVSTVQADIFSSTAHLQNLLHLERHLVTTLHEYVERTETKLNEVRRYLNLFYESYQEVLEEKVPVSSGEDEMVGNPLQAFQLIRRLAVDWGFVRNAMQSDDWNPVRKLTNDYKQLMPSRDDLQGAALALIRLQDTYKLNMTEVAKGEI-LGRA---QGTILTARDLLYLGKESFTAGYYAHAIQWLEEALTKAHQEGNRTAGVSEVMAFYTSAVDMHD-----------RLFERTDIDVADYDTFGHPLKGQHSRVAIAAGRQKPKLG------PDISVHDEQANYMALCRGERLRTHSEESKLTCRLDNQRHPYFLLQPLRIEERGLNPYVVQFHNLLTESQVDLLKIIAEPKLARSMVQAGKGSEVVSVSRTSQNAWVGPHDHPLMAKVYQLVETVTGLSTD---VDNKHAEIVQIANYGMGGHYTPHFDYLLVDKEPEELQYVHPHELESGDRTATLMFYLSDVTRGGATVFPKIGASVWPEKGSAVFWYNLKKNGKEDPMTLHGACPVAVGSKW 507 HSP 3 Score: 164.851 bits (416), Expect = 4.823e-39 Identity = 108/370 (29.19%), Postives = 183/370 (49.46%), Query Frame = 0
Query: 508 KMKSRVFELILLGCLLRVQGEVFSSAGNMREIFALERNLLRTMESHIQKLEDNLREVDTYLKEFHQATSHIPDMAVGPS---DLDLGNPISSFHTVKRLGIFYKKVEESLDQDLQDDFAKSVKRDRRRSIVLPEEEDLQGAAKGLLRIQDVYEIGVPDLSQGKIGQLVSQASLTAQDCLYIGKHCYNEKLLSHSLEWFHQARILAEQEGNRTLDVTTAQKYLDLTAEFHDEQFRSPWGLKQEVFPVPYYKDPNLVQQRKTLNQKRFEDTLNKTKSYVS-KTGEIILKGAPSADV------FNAMCRGTEFRTPAYLSKLYCYTSHQNDLYYLLQPARIEIAHLQPQILVFRDVVTPNDMDLLRDIAKPLL 867
+M+ + +IL+ VQ ++FSS +++ + LER+L+ T+ ++++ E L EV YL F+++ + + V S D +GNP+ +F ++RL + + V ++ D D+ K ++P +DLQGAA L+R+QD Y++ + ++++G+I LTA+D LY+GK + +H+++W +A A QEGNRT V+ + + HD F + ++ Y F L S V+ G K P V + A+CRG RT + SKL C +Q Y+LLQP RIE L P ++ F +++T + +DLL+ IA+P L
Sbjct: 4 RMRQFIVTMILVST---VQADIFSSTAHLQNLLHLERHLVTTLHEYVERTETKLNEVRRYLNLFYESYQEVLEEKVPVSSGEDEMVGNPLQAFQLIRRLAVDWGFVRNAMQSD---DWNPVRKLTNDYKQLMPSRDDLQGAALALIRLQDTYKLNMTEVAKGEILGRAQGTILTARDLLYLGKESFTAGYYAHAIQWLEEALTKAHQEGNRTAGVSEVMAFYTSAVDMHDRLFE-----RTDIDVADY---------------DTFGHPLKGQHSRVAIAAGRQKPKLGPDISVHDEQANYMALCRGERLRTHSEESKLTCRLDNQRHPYFLLQPLRIEERGLNPYVVQFHNLLTESQVDLLKIIAEPKL 347 HSP 4 Score: 83.5741 bits (205), Expect = 1.531e-12 Identity = 36/55 (65.45%), Postives = 41/55 (74.55%), Query Frame = 0
Query: 867 LNNVGGGGRTVFPLIGAGIEPEAGSAVFWYNLNKNLTPDPDTLHGACPIMFGTKW 921
L++V GG TVFP IGA + PE GSAVFWYNL KN DP TLHGACP+ G+KW
Sbjct: 453 LSDVTRGGATVFPKIGASVWPEKGSAVFWYNLKKNGKEDPMTLHGACPVAVGSKW 507 The following BLAST results are available for this feature:
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 11
Pagesback to top
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
Pagesback to top
BLAST of hypothetical protein DAPPUDRAFT_22132 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold103_size370364:231782..256830+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold103_size370364-processed-gene-2.2 ID=snap_masked-scaffold103_size370364-processed-gene-2.2|Name=hypothetical protein DAPPUDRAFT_22132|organism=Tigriopus kingsejongensis|type=gene|length=25049bp|location=Sequence derived from alignment at scaffold103_size370364:231782..256830+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'hypothetical protein DAPPUDRAFT_22132' has the following synonyms
Add to Basket
|