e3 ubiquitin-protein ligase nedd-4, snap_masked-scaffold186_size273091-processed-gene-0.4 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Match: EMLSAG00000007514 (supercontig:LSalAtl2s:LSalAtl2s431:6204:41118:1 gene:EMLSAG00000007514 transcript:EMLSAT00000007514 description:"maker-LSalAtl2s431-snap-gene-0.13") HSP 1 Score: 838.565 bits (2165), Expect = 0.000e+0 Identity = 409/552 (74.09%), Postives = 461/552 (83.51%), Query Frame = 0
Query: 340 SGSAPSLSSVG-GTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIS--TIGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHL-VQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYEMISGA-DLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLF 886
+G+ P +VG LP+ WTMQVAPNGRVF+INH++K+TTWVDPR+GRPS++PSQ N+PNR HEDDLGPLPEGWEERVHTD RIFFIDHNTRTTQWEDPRIS TI GPA+PYNRDYKRKY++LKSQLKKPSNVPNKFEIK+RR++ILEDSF+ + +Q PS KVDVLKTKLWIEFD EIGLDYGG+AREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSG+CNEDH+SYFKFIGR AGMA GKLLDGFFIRPFYKMMLGK + L DMESVDTE +NSL+WI+ENDP ELELTFQVDEE FGTT++RELKP G I VTNE+K++YI +VIQWRFVSR+++QM+ F AGFNELVPLS +KIFDEGELELLMCGIGSIDVKDWK NTVYKGDYHPNHIVIQW+WRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQL+TIE+WGNP+S+PRAHTCFNRLDLPPYE D K I G+ VS S +GL+ + + + N + L F + D LLF
Sbjct: 246 NGNNPPEGAVGPSALPAGWEERQDANGWTMQVAPNGRVFYINHADKETTWVDPRSGRPSALPSQANVPNRRHEDDLGPLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRISNPTIAGPAVPYNRDYKRKYEFLKSQLKKPSNVPNKFEIKIRRRYILEDSFQKIFMQFPSHKVDVLKTKLWIEFDGEIGLDYGGVAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGVCNEDHLSYFKFIGRXAGMAXXXGKLLDGFFIRPFYKMMLGKGMELSDMESVDTEXFNSLMWIRENDPQELELTFQVDEENFGTTVTRELKPGGKNIPVTNENKDEYIHQVIQWRFVSRIQKQMDNFLAGFNELVPLSFIKIFDEGELELLMCGIGSIDVKDWKQNTVYKGDYHPNHIVIQWYWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIERWGNPSSYPRAHTCFNRLDLPPYESYQQLRDRLIKAIEGSEGFAGGVSSSHPR--VILGLKQIHILKEIRKNCFVLVSFTLSQH-DKPKLLF 794 HSP 2 Score: 666.766 bits (1719), Expect = 0.000e+0 Identity = 365/799 (45.68%), Postives = 489/799 (61.20%), Query Frame = 0
Query: 809 CFNRLDLPPYEMISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDL----KDVATTGHLPDTFYTLRKRSPKSKVRGRLFLQ---IENKP--DSNPTNTPSSQPQTEPHATGASVAPLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQ-EKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNH----LKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDG 1593
+ R L ++I+G LAKKDIFGASDPY+ + L + +T K++LNP W F+FR P H L+ +VFDENR+TRDDFLG V + L ++ +Y LR R+ +SKV+G L L I P DS+ + + +E + V + P + PE WE P+ P +G ++ ER H P PT D+ +E N P V P S+ P QD +GW ++VAPN GRVF+INH +ETTWVDPR+ RPS LP ++ + P++ +D L LP+GWEE++ DGR FIDH + TQWEDPRI+ G + ++ +YK+KYE +L P +P K I +RR +I +DSF++I P+H LK +LW+EF+GE GLD+GG+AREWF+LLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + DHL+YF+FIG+ AGMA G LLDGFFIRPFYKMML + + L DMESVD E +NSL++I+++DP+ LEL+FQVDE+ G TV+ +L P G ++ VT NK +YI +VI WRFVSR+Q+QM F GF +PL+ + +FDEGELELL+CGI +ID++DW ++TVY+G YHPNH V+QW+WR VLSF+N+MR+RLLQFVTGTSRVPMNGF L GSN + F I+ +G+P S+PR+HTCFNRLDLP Y SY++LR RL+ A++ +E F G
Sbjct: 18 SWERTRLLRLKVIAGHQLAKKDIFGASDPYLRIDLMSVHQDGIVD-SVLTKTRKRTLNPKWDEEFIFRVKPGEHKLVLEVFDENRLTRDDFLGKVELGLTGXPRETGQXXPPAHKYYLLRPRTSRSKVKGHLQLYHAFISGSPGEDSSEESAAGAGAXSEEPVSFTEVE-----GSSDTPSGSSPEDWE---------------------IVPNPISQEGPPEEGEGVELS-------------ERSHPPPPPPPPPPPPPTNDEEDE-------------NGNNPPEGAVGP--SALPAGWEERQDA----NGWTMQVAPN-GRVFYINHADKETTWVDPRSGRPSALPSQA---NVPNRRHEDDLGPLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRISNPTIAGPAVPYNRDYKRKYEFLKSQLKKPSNVPNKFEIKIRRRYILEDSFQKI---FMQFPSHKVDVLKTKLWIEFDGEIGLDYGGVAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGVCNEDHLSYFKFIGRXAGMAXXXGKLLDGFFIRPFYKMMLGKGMELSDMESVDTEXFNSLMWIRENDPQELELTFQVDEENFGTTVTRELKPGGKNIPVTNENKDEYIHQVIQWRFVSRIQKQMDNFLAGFNELVPLSFIKIFDEGELELLMCGIGSIDVKDWKQNTVYKGDYHPNHIVIQWYWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIERWGNPSSYPRAHTCFNRLDLPPYESYQQLRDRLIKAIEGSEGFAG 750 HSP 3 Score: 258.455 bits (659), Expect = 4.324e-72 Identity = 156/310 (50.32%), Postives = 187/310 (60.32%), Query Frame = 0
Query: 5 RIVGYVPPPGSTDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPT-KQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSEG----WEIVGNSEQTVDTTVTGFEELPAPIRGGG--NEGSG-------------------------------DPTAGLV----LPAGWEERQDA---------NGRTYYVNHVARSTQWQHPALTDNTAATAAVNENN 263
RI+GYVPPPGS +RT+LLRLKVIAGH LAKKDIFGASDPYLRIDL++V + + DSVLTKT+KRTLNPKW+EEFIFRVKP HKLVLEVFDENRLTRDDFLG VEL L PRE+ P K YLLRPR++RSKVKG+LQLYHA+++ S D++ E G A SE E+ G+S+ ++ +E +P PI G EG G +P G V LPAGWEERQDA NGR +Y+NH + T W P +A + N N
Sbjct: 8 RILGYVPPPGSWERTRLLRLKVIAGHQLAKKDIFGASDPYLRIDLMSVHQDGIVDSVLTKTRKRTLNPKWDEEFIFRVKPGEHKLVLEVFDENRLTRDDFLGKVELGLTGXPRETGQXXPPAHKYYLLRPRTSRSKVKGHLQLYHAFIS---GSPGEDSSEESAAGAGAXSEEPVSFTEVEGSSDTPSGSSPEDWEIVPNPISQEGPPEEGEGVELSERSHPPPPPPPPPPPPPTNDEEDENGNNPPEGAVGPSALPAGWEERQDANGWTMQVAPNGRVFYINHADKETTWVDPRSGRPSALPSQANVPN 314
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Match: EMLSAG00000011039 (supercontig:LSalAtl2s:LSalAtl2s744:109377:113126:-1 gene:EMLSAG00000011039 transcript:EMLSAT00000011039 description:"maker-LSalAtl2s744-snap-gene-1.41") HSP 1 Score: 423.705 bits (1088), Expect = 3.569e-129 Identity = 227/484 (46.90%), Postives = 300/484 (61.98%), Query Frame = 0
Query: 366 WTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPR---ISTIGGPAIPYNR--DYKRKYDYLKSQLK---KPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAR--------------------EWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAE--LELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
W +V PNGRV+F+NH + T W DPRT QG + ED PLP GWE R +F+DHNTRTT ++DPR + GG Y Y+R + + SQ + + +++P+ ++ V RQ + EDSF L+++P+ + L+ +L+I F E GLDYGG AR EWF+LLS E+ NP Y LFEY+ +NY+LQINP S N DH+ YFKFIGR MA+YHGK + F PFYK ML K + +KD+ES+D EYYNSL+WIK+N+ E LE+ F VD E G ELKP G+++ VT E+K +YI+ V +WR +EEQ F GFNE+VPL L+ FDE ELEL++CG+ IDV DW+ +++Y+ Y N IQWFW+ V S ++E RSRLLQFV GT RVP+ GF EL GSNG Q + +EK G + PR+HTCFNRLDLPPY+
Sbjct: 390 WEKRVEPNGRVYFVNHKNRTTQWEDPRT--------QGQV----KED---PLPPGWEIRFMAGGVPYFVDHNTRTTTFQDPRPGATNAKGGAKGAYGVPVQYERSFRWKLSQFRYLCQSNSLPSHIKLSVTRQTLFEDSFHQLMRLPAFE---LRRRLYIIFRGEEGLDYGGXARXVDMLLLLPCSYVNLIYPLREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPAS-YVNPDHLHYFKFIGRFIAMALYHGKFIYSGFTMPFYKRMLNKKLIMKDIESIDPEYYNSLMWIKDNNIEECGLEMYFCVDFEVLGEIKPHELKPGGSELKVTEENKEEYIEIVCEWRMSRGIEEQTKSFLEGFNEVVPLEWLQYFDERELELMLCGMQVIDVDDWQRHSIYR-HYTKNSKQIQWFWQFVRSMDHEKRSRLLQFVCGTCRVPVGGFAELMGSNGAQRFCVEKVGKDSWLPRSHTCFNRLDLPPYK 853 HSP 2 Score: 402.519 bits (1033), Expect = 1.594e-121 Identity = 218/525 (41.52%), Postives = 310/525 (59.05%), Query Frame = 0
Query: 1099 PERTIP--QDTEG-LPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSF-------MTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAR--------------------EWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEP--LELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAF 1591
P+++ P +D +G L GW +V PN GRV+F+NH R T W DPRT + + +DP LP GWE + G F+DH + T ++DPR + KG + ++ +++ K +F + L LP + + V R +F+DSF ++ + + L+ RL++ F GE GLD+GG AR EWFFLLS E+ NP Y LFEY+ ++Y+LQINP S + +PDHL+YF+FIG+ MA+YHG + F PFYK ML++K+ + D+ES+D EYYNSL++IKD++ E LE+ F VD + LG +L P G+++ VTE NK +YI+ V WR ++EQ ++F GF +PL L FDE ELEL+LCG+ ID+ DW +H++YR Y N + +QWFW+ V S ++ R+RLLQFV GT RVP+ GF L GSN A+ FC++ G PRSHTCFNRLDLP Y SY +L +L A++ E F
Sbjct: 372 PDQSRPSEEDPQGPLMDGWEKRVEPN-GRVYFVNHKNRTTQWEDPRT--------QGQVKEDP---------LPPGWEIRFMAGGVPYFVDHNTRTTTFQDPRPGATNAKGGAKGAYGVPVQYERSFRWKLSQF-RYLCQSNSLPSHIKLSVTRQTLFEDSFHQLMRLPAFE---LRRRLYIIFRGEEGLDYGGXARXVDMLLLLPCSYVNLIYPLREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYV-NPDHLHYFKFIGRFIAMALYHGKFIYSGFTMPFYKRMLNKKLIMKDIESIDPEYYNSLMWIKDNNIEECGLEMYFCVDFEVLGEIKPHELKPGGSELKVTEENKEEYIEIVCEWRMSRGIEEQTKSFLEGFNEVVPLEWLQYFDERELELMLCGMQVIDVDDWQRHSIYRH-YTKNSKQIQWFWQFVRSMDHEKRSRLLQFVCGTCRVPVGGFAELMGSNGAQRFCVEKVGKDSWLPRSHTCFNRLDLPPYKSYDQLVEKLNFAIEETEGF 872
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Match: EMLSAG00000006171 (supercontig:LSalAtl2s:LSalAtl2s336:651240:657086:1 gene:EMLSAG00000006171 transcript:EMLSAT00000006171 description:"maker-LSalAtl2s336-augustus-gene-6.36") HSP 1 Score: 357.836 bits (917), Expect = 4.381e-105 Identity = 184/366 (50.27%), Postives = 245/366 (66.94%), Query Frame = 0
Query: 458 YNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAEL-ELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGS---NGPQLYTIEKWGNP-NSFPRAHTCFNRLDLPPY 818
+ +D K L+S L ++V RQ + EDS+R +V++ + V++ KL ++F NE GLDYGG+AREW YLLS EM NPYYGLF+YS+ D YTLQINP S + N DH+SYF F+GRI GMAV+HG +DG F PFYKMML K I L D+E+VD + + SL WI ND + + + TF V+ E FG T ELK NG I V +K +Y+K +Q+R+ +E+Q + GFNEL+P +LLK FDE ELEL++ GIG+ID+ DWK +T K V+QWFW +V S+++EMR+RLLQFVTG+SRVP+ GFK+L GS GP+ +T+ P +S P+A TCFNRLDLP Y
Sbjct: 549 FKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKM---RYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLK-QCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAY 909 HSP 2 Score: 325.865 bits (834), Expect = 5.549e-94 Identity = 165/359 (45.96%), Postives = 232/359 (64.62%), Query Frame = 0
Query: 1238 IPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEP-LELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEA---FCIKGFGSP-DSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAF 1591
+ V R +F+DS+ R I + ++ +L V+F E GLD+GG+AREW +LLS EM NPYYGLF+YS+ D YTLQINP S + +PDHL+YF F+G++ GMAV+HGH +DG F PFYKMML++ I L D+E+VD + + SL +I +D ++ +F V+ + G+T +L G D+ V E+NK++Y+ + +R+ +++Q + Q+GF IP N L FDE ELEL++ GI IDI DW HT + VVQWFW V S+S++MRARLLQFVTG+SRVP+ GF LQGS F + +P DS P++ TCFNRLDLP Y S++ + +L AV+ F
Sbjct: 576 LEVSRQEVFEDSY---RQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLK-QCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGF 929 HSP 3 Score: 110.153 bits (274), Expect = 2.128e-24 Identity = 94/391 (24.04%), Postives = 170/391 (43.48%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITR----DDFLGLVIVDLKDVATTGHLPDTFYTLRKRSPKSK----VRGRLFLQIENKPDSNPTNTPSSQPQTEPHATGASVAPLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIEL-GSPHTAIPTGNTRETPSNVVEPCQSSAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIAL 1201
++ +LAK+D+F DP+ V + G+ V+ K + +P W + +P+ + V+++ ++ + FLG V + + + DT Y +K V+G++ + + ++ + H TG+ A + + N + LPEGWEER++ +GR YYV+H R P+ G+ G + + S + + + G ++ L G+ +P N +++PS++ +P S P I Q + LP G+ +K +G++++I+ T +TTW DPR R S E+ +L LP GWE+++T R F++H TQ+ DPRI L
Sbjct: 12 ILCARNLAKRDLFRLPDPFARVDVD------ATGVTHVSEAAKNTTDPKWNTHYDLHLSPN-ESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQ---RIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSR---------------DGHGTGSLNAVVDTLGN-LTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQ-PDNLPPGYIMKTT-EHGQIYYIHEGTGQTTWHDPRLTRALT-----------SNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPL 363 HSP 4 Score: 102.834 bits (255), Expect = 4.374e-22 Identity = 75/245 (30.61%), Postives = 115/245 (46.94%), Query Frame = 0
Query: 210 GDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNENNASEL--SRRRVHISMDEQRPSLDIGRSRLGSDSV-IDELHSLNINRGE---SVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRI 448
G+ T LP GWEER+ +GR YYVNHV R+TQW P + T + + S + ++S + + G++S+ I H+L N + +V+ D PS+ S+ PRR + T P + LP + M+ +G++++I+ +TTW DPR R + + N LGPLP GWE+RV +R +F++HN TTQ+ DPRI
Sbjct: 149 GNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQ-----PRRHQI-------------TQP-----DNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELN---------LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Match: EMLSAG00000004991 (supercontig:LSalAtl2s:LSalAtl2s260:299952:311628:1 gene:EMLSAG00000004991 transcript:EMLSAT00000004991 description:"maker-LSalAtl2s260-augustus-gene-3.39") HSP 1 Score: 294.278 bits (752), Expect = 1.963e-80 Identity = 150/382 (39.27%), Postives = 222/382 (58.12%), Query Frame = 0
Query: 1210 TFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPL--ELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCI-KGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNA 1588
D + K++Y R ++ + + VRR+++F++SF R + + P K R ++ FEGE G D GGL REW+ ++S+E+FNP Y LF+ S D T I S +P+HL+YF+F+G+V A+Y LL+ +F R FYK +L + + DMES D +Y L ++ +H L +L+F + GVT DLIP G ++TVTE KLDYI V + +++Q+ AF GF IP + +F+E ELELLL G+ IDI D +T Y Y N + WFWR++ SF +A+ +QFVTG+S+VP+ GF L+G N + F I + S D P +HTCFN+LDLP Y +Y +LR+ L+ A+
Sbjct: 2575 VLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESF---RELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYE-SSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYH-KYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHEC 2951 HSP 2 Score: 286.189 bits (731), Expect = 5.961e-78 Identity = 147/364 (40.38%), Postives = 220/364 (60.44%), Query Frame = 0
Query: 461 DYKRKYDYLKSQLKKPSNVPNK--FEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAEL--ELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWG-NPNSFPRAHTCFNRLDLPPYE 819
D+ K Y +++L++ + + VRR+ + E+SFR L + + K + +I F+ E G D GGL REW+ ++S+E+FNP Y LF+ S D T I S N +H+ YFKF+GR+ A+Y KLL+ +F R FYK +LGK + DMES D +Y L ++ + ++L +LTF + ++FG T R+L PNG I VT E+K DYI+ V Q + + +Q++ F GF ++P L+ IF+E ELELL+ G+ +ID+ D K+NT Y Y N + I WFWR + SF+ +++ +QFVTG+S+VP+ GF L G NG Q + I + + + P AHTCFN+LDLP YE
Sbjct: 2577 DFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEX---KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYE-SSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYH-KYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE 2935
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Match: EMLSAG00000003493 (supercontig:LSalAtl2s:LSalAtl2s190:418567:426512:1 gene:EMLSAG00000003493 transcript:EMLSAT00000003493 description:"maker-LSalAtl2s190-snap-gene-4.16") HSP 1 Score: 187.963 bits (476), Expect = 1.989e-48 Identity = 118/374 (31.55%), Postives = 202/374 (54.01%), Query Frame = 0
Query: 1231 MLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEG-----ESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDR---KITLIDMESVDVEYYNSLVYIKDHD--PEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDA-IDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGS-PDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFD 1592
++ P + + +RR I++D+ E++ +++PN +K ++ VEF+ E+G+D GGL RE+ L K F+P G F + D L NP + D L ++ FIG++ G A+Y L++ F +L++ K+ + ++S+D E Y +L+Y+K++D + L L F + + +G T E+L G+ + VT N+ +YI + +++ +++ Q AF++G I L+ L +F+ EL+L++ G + ID+ D HT Y GGY +H V FW V S + + LL+FVT SR P+ GF L FC+ G+ P P + TC N L LP + K L+ +L+ A+++ FD
Sbjct: 651 LMGPSIDVTIRRKFIYEDALEKLS--PEAEPN-MKLKMRVEFKNIAGLREAGIDGGGLFREFLGELVKTSFDPNRGFFRLTK--DQRLYPNPSVHKIYEDFLPHYFFIGRMLGKALYENMLVEFPLASFFLHKLLEKSSVKVDIDHLQSLDPELYRNLLYLKNYDGCVQDLGLDFTIVNEEVGTTSVEELKRNGSSIPVTNVNRTEYIHLIANYKLNKQIRLQSNAFRQGLSDVINLDWLKMFNYKELQLVISGAETEIDVSDLRNHTNYTGGYAVDHPTVVIFWNVVESMTETQKRHLLKFVTSCSRPPLLGFKELDP-----PFCVHNAGAEPHRLPTASTCMNLLKLPNFEEEKILKDKLLYAIESGSGFD 1014 HSP 2 Score: 181.8 bits (460), Expect = 1.733e-46 Identity = 120/347 (34.58%), Postives = 184/347 (53.03%), Query Frame = 0
Query: 484 EIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDN-----EIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKT---ITLKDMESVDTEYYNSLLWIKENDPA--ELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGS-IDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
++ +RR+FI ED+ L P ++ + +K K+ +EF N E G+D GGL RE+ L K F+P G F T + L NP ED + ++ FIGR+ G A+Y L++ F +L K+ + + ++S+D E Y +LL++K D +L L F + E+ GTT ELK NG+ I VTN ++ +YI + ++ ++ Q N F+ G ++++ L LK+F+ EL+L++ G + IDV D + +T Y G Y +H + FW VV S + LL+FVT SR P+ GFKEL P P+ P A TC N L LP +E
Sbjct: 657 DVTIRRKFIYEDALEKLS--PEAEPN-MKLKMRVEFKNIAGLREAGIDGGGLFREFLGELVKTSFDPNRGFFR--LTKDQRLYPNPSVHKIYEDFLPHYFFIGRMLGKALYENMLVEFPLASFFLHKLLEKSSVKVDIDHLQSLDPELYRNLLYLKNYDGCVQDLGLDFTIVNEEVGTTSVEELKRNGSSIPVTNVNRTEYIHLIANYKLNKQIRLQSNAFRQGLSDVINLDWLKMFNYKELQLVISGAETEIDVSDLRNHTNYTGGYAVDHPTVVIFWNVVESMTETQKRHLLKFVTSCSRPPLLGFKEL----DPPFCVHNAGAEPHRLPTASTCMNLLKLPNFE 994
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Match: EMLSAG00000008825 (supercontig:LSalAtl2s:LSalAtl2s54:414186:494517:-1 gene:EMLSAG00000008825 transcript:EMLSAT00000008825 description:"maker-LSalAtl2s54-augustus-gene-5.10") HSP 1 Score: 162.54 bits (410), Expect = 2.394e-40 Identity = 116/377 (30.77%), Postives = 189/377 (50.13%), Query Frame = 0
Query: 1238 IPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEG-----ESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKIT-----LIDMESVDVEYYNSLVYIK--DHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDA-IDIQDWMKHTVYRGGYHPNHQVVQWFWRSV-LSFSNQMRARLLQFVTGTSRVPMNGFVALQ--------------------GSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSR 1580
I V R I +D + R + + LK + V+F E+G+D G+ +E+ K +F+P GL + + D L +P S + ++L FEF+G++ G AVY G +++ F F +L + + L ++ S+D E Y +L YIK D + L+L+F D+D LG V+ +LI G V V + NK+ YI V + S+++EQ+ AF RGF+ +P++ L +F E+ L+ G + +D++D +HT Y GG+H +H+V+ W W + F ++ R+ L+FVT S+ P+ GF L+ GS +K + P + TCFN L LP Y LR +
Sbjct: 615 ITVHRSRIVEDGY---RHLGKLSSRSLKGVIRVKFINTQGLDEAGIDQDGVFKEFLEETIKRVFDP--GLNLFCSTSDNRLYPSPTSHLTE-NYLQLFEFVGKMIGKAVYEGIVVEIPFAPFFISQILSKDHSAYYSYLDELPSMDPELYKNLTYIKYFDGNVTDLDLTFSYDQDVLGRVVTHELIEGGRSVLVNDCNKISYIHHVAQFVLHSQIKEQVAAFTRGFRAILPIDWLNLFSPPEVLRLISGDNLPLDLKDLRRHTHYYGGFHDSHKVIVWLWEILDRDFDDKERSSFLKFVTSCSKPPLLGFENLEPAFSIRCVEVAEDEDDGDTVGSVLRGFLALKKRDPVNRLPTASTCFNLLKLPNYQKKATLRDK 985 HSP 2 Score: 159.844 bits (403), Expect = 1.811e-39 Identity = 114/369 (30.89%), Postives = 189/369 (51.22%), Query Frame = 0
Query: 485 IKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDN-----EIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTIT-----LKDMESVDTEYYNSLLWIK--ENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGS-IDVKDWKTNTVYKGDYHPNHIVIQWFWRVV-LSFNNEMRSRLLQFVTGTSRVPMNGFKEL--------------------YGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
I V R I+ED +RHL ++ S LK + ++F N E G+D G+ +E+ K +F+P GL + +T + L +P S + E+++ F+F+G++ G AVY G +++ F F +L K + L ++ S+D E Y +L +IK + + +L+LTF D++ G ++ EL G + V + +K YI V Q+ S+++EQ+ F GF ++P+ L +F E+ L+ G +D+KD + +T Y G +H +H VI W W ++ F+++ RS L+FVT S+ P+ GF+ L GS ++K N P A TCFN L LP Y+
Sbjct: 615 ITVHRSRIVEDGYRHLGKLSSRS---LKGVIRVKFINTQGLDEAGIDQDGVFKEFLEETIKRVFDP--GLNLFCSTSDNRLYPSPTSHL-TENYLQLFEFVGKMIGKAVYEGIVVEIPFAPFFISQILSKDHSAYYSYLDELPSMDPELYKNLTYIKYFDGNVTDLDLTFSYDQDVLGRVVTHELIEGGRSVLVNDCNKISYIHHVAQFVLHSQIKEQVAAFTRGFRAILPIDWLNLFSPPEVLRLISGDNLPLDLKDLRRHTHYYGGFHDSHKVIVWLWEILDRDFDDKERSSFLKFVTSCSKPPLLGFENLEPAFSIRCVEVAEDEDDGDTVGSVLRGFLALKKRDPVNRLPTASTCFNLLKLPNYQ 977
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Match: EMLSAG00000007808 (supercontig:LSalAtl2s:LSalAtl2s458:200727:208701:-1 gene:EMLSAG00000007808 transcript:EMLSAT00000007808 description:"maker-LSalAtl2s458-snap-gene-2.45") HSP 1 Score: 131.339 bits (329), Expect = 2.873e-31 Identity = 103/398 (25.88%), Postives = 178/398 (44.72%), Query Frame = 0
Query: 1202 SATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDV-EYYNSLV-YIKDHDPEPLELSF--QVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
+A K MTFD + + F + +G L + I V R + S + + P L + F GE G+++GGL REW+ LL KE+ + + ++ NP+ +Y P + F+ Q+ G+ + H VD E+Y S + YI + E L+L F ++ +++ + S++L P G + VT NK +Y+ + +R R++ + F+ G ++ L FDE E++LL G ID D KH + +H++++WFW + S + + L+F+TG+SR+P NGF L F I+ P SH C N L++ ++S++ S + A+ E + G +
Sbjct: 327 AANKKCSMTFDEKLVEFFSNF-EYMGELIFLMQSI-INVDRHCLLYSSIKSLEQCWWIGP------LKISFSGEQGIEYGGLRREWYTLLWKELSDT-----KIFITNERGTGANPMFRLYLPFSFSS-SFLYQLKGINIGXRHF------------------------KVDYPEFYTSRIDYIVKNKVEDLDLYFTDEIYDEKGHLIESKNLKPSGKKIKVTNFNKFEYLHLLAKYRLSDRIKGPLTKFKTGLHYYVSETYLANFDESEMDLLFSGFSTIDFNDLKKH---HNCQNVSHKIMEWFWLVLEHLSEEQKLLFLKFITGSSRLPPNGFKDL-----CPVFTIRQLNEEGKLPSSHVCANVLNIHPHSSFEAFESSFITAI--TEGYVGFE 676 HSP 2 Score: 122.479 bits (306), Expect = 1.768e-28 Identity = 90/307 (29.32%), Postives = 146/307 (47.56%), Query Frame = 0
Query: 515 LWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLL-WIKENDPAELELTF--QVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPY 818
L I F E G++YGGL REW+ LL KE+ +D N N Y F F F + G I + + E+Y S + +I +N +L+L F ++ +E+ S+ LKP+G KI VTN +K +Y+ + ++R R++ + +FK G + V + L FDE E++LL G +ID D K + + +H +++WFW V+ + E + L+F+TG+SR+P NGFK+L ++TI + P +H C N L++ P+
Sbjct: 385 LKISFSGEQGIEYGGLRREWYTLLWKEL------------SDTKIFITNERGTGANPMFRLYLPFS-----------------FSSSFLYQLKGINIGXRHFKVDYPEFYTSRIDYIVKNKVEDLDLYFTDEIYDEKGHLIESKNLKPSGKKIKVTNFNKFEYLHLLAKYRLSDRIKGPLTKFKTGLHYYVSETYLANFDESEMDLLFSGFSTIDFNDLKK---HHNCQNVSHKIMEWFWLVLEHLSEEQKLLFLKFITGSSRLPPNGFKDL-----CPVFTIRQLNEEGKLPSSHVCANVLNIHPH 654
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Match: EMLSAG00000002245 (supercontig:LSalAtl2s:LSalAtl2s1426:7958:26313:1 gene:EMLSAG00000002245 transcript:EMLSAT00000002245 description:"maker-LSalAtl2s1426-augustus-gene-0.12") HSP 1 Score: 109.768 bits (273), Expect = 4.485e-24 Identity = 103/380 (27.11%), Postives = 180/380 (47.37%), Query Frame = 0
Query: 1234 PKVTI----PVRRDHIFQDSFERIRTIAQSDPNHLKA--RLW-VEFEGESGLDFGGL----AREWFFLLSKEMFNPYY----GLFEYSANDD-YTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIK-DHDPEPLELSFQVDEDRLGVTVSED---LIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDS-FPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFD 1592
P+VT+ P+ IF E++ I DP L+ R W V+ GE+ D GG+ E LSK++ N G+ + +N D Y L + S + + +F F+G + G+A+ + +K++L+ K T D+E VD+ Y SL Y+ +P+ E S + +G++ S L P + ++ NK +Y+ ++ RF + Q+ A ++G P L + +E L+CG + + K YR P+++V+ W W + F+N ++ L FV+G SR+P + F + ++ F I P++ P + TCF +L LP YTS + +R+RL+ A+++ D
Sbjct: 2973 PQVTVRRLFPIGSSSIFSQIAEQVFYI---DPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKV----DMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRD-MDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAF------DVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTID 3337 HSP 2 Score: 103.99 bits (258), Expect = 3.254e-22 Identity = 77/248 (31.05%), Postives = 125/248 (50.40%), Query Frame = 0
Query: 576 YFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKEN-DPAELELTFQVDEEQFGTTIS-RE--LKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNP-NSFPRAHTCFNRLDLPPY 818
+F F+G + G+A+ K + +K++L T KD+E VD Y SL ++ + +P E + + G + S RE L P KI ++ E+K++Y+K + RF + Q+ + G + L P +LK+ +E L+CG S + K Y+ D P++ VI W W ++ FNN+++ L FV+G SR+P + F + Q + I + P N P A TCF +L LPPY
Sbjct: 3077 HFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYR-DMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAF------DVSQRFQILRVERPENGLPTAQTCFFQLRLPPY 3316
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Match: EMLSAG00000009470 (supercontig:LSalAtl2s:LSalAtl2s60:50964:63500:-1 gene:EMLSAG00000009470 transcript:EMLSAT00000009470 description:"maker-LSalAtl2s60-augustus-gene-0.20") HSP 1 Score: 103.219 bits (256), Expect = 4.540e-22 Identity = 107/419 (25.54%), Postives = 181/419 (43.20%), Query Frame = 0
Query: 1241 RRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFE-----------YSANDDYTLQ--------INPLSGIYHPDHL-------------NYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITL--IDMESVDVEYYNSLVYIK---------DHDP-----EPLEL--SFQVD----EDRLGVTVSE------DLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRG-----GYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDS-----FPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAE 1589
R D++ + S + + +A S ++ L +++E E G G E++ L+SKE+ GL++ +N+D I+ G+Y P+ + N F F+G+ AV ++D F +PFY+ +L + TL D+ +VD +++ ++ + DP E LE S +D ED LG+ + DL G D+ V N Y+ V HW + + QM+A + GF+ P++ L +F ELE + CG + W T+ G++ +++ + S++ + + LQFVTG R+P+ GF AL N K F + D+ P TC N L LP Y+S K +R +L +A +
Sbjct: 1330 RDDNLLKQSEQVMNELAPS-----RSLLEIQYENEVGTGLGP-TLEFYTLVSKELQKADLGLWKGETVKISNEDVMDSNEDNERNDKSSCIEYIHSSVGLY-PNPMARNVKSSQRSKIKNKFTFLGKFIAKAVLDNRMIDMPFSQPFYQWLLREESTLGISDLRNVDPTIASTISSLEGIARKKRKLEQDPQISPDEKLEQIKSLTMDGCPIED-LGLDFTLPGYPNIDLRKGGKDIPVNIDNLHQYVGLVSHWLLIEGISFQMEALRDGFESVFPISTLQMFYPEELEQIFCGTGQGNFNKWDYKTLVESTKPDHGFNSESPALKYLLEVLESYNIDEQRQFLQFVTGCPRLPVGGFKAL---NPPLTIVKKSFDNADADPDDYLPSVMTCVNYLKLPDYSSKKIMREKLRIAAMEGQ 1737 HSP 2 Score: 97.0561 bits (240), Expect = 2.911e-20 Identity = 96/381 (25.20%), Postives = 164/381 (43.04%), Query Frame = 0
Query: 512 KTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGM-CNEDH--------ISY---------------------------FKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGK--TITLKDMESVDTEYYNSLLWIK---------ENDPA-----ELE----LTFQ-VDEEQFGTTISR------ELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNH------IVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNS-----FPRAHTCFNRLDLPPY 818
++ L I+++NE+G G E++ L+SKE+ GL++ T++I+ M NED+ I Y F F+G+ AV +++D F +PFY+ +L + T+ + D+ +VD +++ ++ E DP +LE LT E G + +L+ G I V ++ + Y+ V W + + QM + GF + P+S L++F ELE + CG G + W T+ + P+H +++ V+ S+N + + + LQFVTG R+P+ GFK L N P + + N ++ P TC N L LP Y
Sbjct: 1349 RSLLEIQYENEVGTGLGP-TLEFYTLVSKELQKADLGLWK-----GETVKISNEDVMDSNEDNERNDKSSCIEYIHSSVGLYPNPMARNVKSSQRSKIKNKFTFLGKFIAKAVLDNRMIDMPFSQPFYQWLLREESTLGISDLRNVDPTIASTISSLEGIARKKRKLEQDPQISPDEKLEQIKSLTMDGCPIEDLGLDFTLPGYPNIDLRKGGKDIPVNIDNLHQYVGLVSHWLLIEGISFQMEALRDGFESVFPISTLQMFYPEELEQIFCGTGQGNFNKWDYKTLVEST-KPDHGFNSESPALKYLLEVLESYNIDEQRQFLQFVTGCPRLPVGGFKAL---NPPLTIVKKSFDNADADPDDYLPSVMTCVNYLKLPDY 1719
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Match: EMLSAG00000012949 (supercontig:LSalAtl2s:LSalAtl2s98:1049432:1083512:-1 gene:EMLSAG00000012949 transcript:EMLSAT00000012949 description:"maker-LSalAtl2s98-augustus-gene-10.16") HSP 1 Score: 93.9745 bits (232), Expect = 3.186e-19 Identity = 75/288 (26.04%), Postives = 134/288 (46.53%), Query Frame = 0
Query: 507 KVDVLKTKLW-IEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLF-------EYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDP-----AELELTFQV-----DEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVP 776
K +L ++W ++F E D GG E + E+ N L + + T +NP + + + + F+F+G + G+AV G L P +K++ G + L D+ VD +Y LL I++ DP ++++ F + Q T SR VT E++N+Y+K IQ+R ++ + ++ + G + ++P+ LL +F ELE+++C G ID + +++WFW V+ F+ RS L+FV G +R+P
Sbjct: 4450 KCLLLPHRVWKVKFIGESVDDCGGGYSESIAEMCDELQNGTLSLLISTPNGRDEAGTSRDCFLLNP--ALNSSLYKNMFRFLGILMGIAVRTGSPLSLNLAEPMWKLLAGACLNLNDITEVDKDYVPGLLCIRDMDPDPKSFLDMDMPFSTPSCAGHDVQLSTRYSR----------VTLENRNEYVKLAIQYR-LNEFDFAVSAIREGMSRVIPVPLLSLFTGYELEVMVC--GRID---------------SSAPLVEWFWEVMEEFSTSERSLFLRFVWGRTRLP 4707 HSP 2 Score: 87.0409 bits (214), Expect = 4.139e-17 Identity = 82/284 (28.87%), Postives = 124/284 (43.66%), Query Frame = 0
Query: 1264 LKARLW-VEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSAN---------DDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVT-------VTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCG-IDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVP 1529
L R+W V+F GES D GG E + E+ N L + N D + L S +Y N F F+G + G+AV G L P +K++ + L D+ VD +Y L+ I+D DP+P +D D T S G DV VT N+ +Y+ I +R ++ + A + G R IP+ L +F ELE+++CG ID+ + +V+WFW + FS R+ L+FV G +R+P
Sbjct: 4454 LPHRVWKVKFIGESVDDCGGGYSESIAEMCDELQNGTLSLLISTPNGRDEAGTSRDCFLLNPALNSSLYK----NMFRFLGILMGIAVRTGSPLSLNLAEPMWKLLAGACLNLNDITEVDKDYVPGLLCIRDMDPDPKSF---LDMDMPFSTPS----CAGHDVQLSTRYSRVTLENRNEYVKLAIQYR-LNEFDFAVSAIREGMSRVIPVPLLSLFTGYELEVMVCGRIDS------------------SAPLVEWFWEVMEEFSTSERSLFLRFVWGRTRLP 4707
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Match: gi|32172436|sp|P46935.3|NEDD4_MOUSE (RecName: Full=E3 ubiquitin-protein ligase NEDD4; AltName: Full=HECT-type E3 ubiquitin transferase NEDD4; AltName: Full=Neural precursor cell expressed developmentally down-regulated protein 4; Short=NEDD-4) HSP 1 Score: 794.653 bits (2051), Expect = 0.000e+0 Identity = 425/828 (51.33%), Postives = 536/828 (64.73%), Query Frame = 0
Query: 17 DRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVT-VDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPT--KQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPA-LTDNTAATAAVNENNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTT-------TSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTE------------GLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRISTIG--GPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
D T+++R+KVIAG GLAKKDI GASDPY+R+ L + G + SV TKT K++LNPKW EE +FRV P H+++ EVFDENRLTRDDFLG V++ L LP E+ P K ++L PRS +S+VKG L+L Y+ + + +E EELE GW ++ + LP GWEERQD GRTYYVNH +R TQW+ P+ D T + A R IS D P R + ++ E + + G++V +P T T A G TS P S+ + SS GG+L + + E GLP W + GR ++++H+ K TTW P ++ + +DLGPLP GWEER HTD R+FFI+HN + TQWEDPR+ + GPA+PY+RDYKRKY++ + +LKK +++PNKFE+K+RR ILEDS+R ++ + + D+LK +LWIEFD E GLDYGG+AREWF+L+SKEMFNPYYGLFEYSATDNYTLQINP SG+CNEDH+SYFKFIGR+AGMAVYHGKLLDGFFIRPFYKMML K ITL DMESVD+EYY+SL WI ENDP EL+L F +DEE FG T ELK G++I VTN++K +YI VIQWRFV+R+++QM FK GF EL+P L+KIFDE ELELLMCG+G +DV DW+ +T YK Y NH VI WFW+ V ++E R RLLQFVTGTSRVPMNGF ELYGSNGPQ +T+E+WG P+ PRAHTCFNRLDLPPYE
Sbjct: 75 DDTRVVRVKVIAGIGLAKKDILGASDPYVRVTLYDPMSG--ILTSVQTKTIKKSLNPKWNEEILFRVLPQRHRILFEVFDENRLTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSEDENADQAEELE------PGWVVLDQPDAATHLPHP--------------------PEPSPLPPGWEERQDVLGRTYYVNHESRRTQWKRPSPDDDLTDEDNDDMQLQAQRAFTTRRQISEDVDGPD---NRESPENWEIVREDENTEYS-GQAVQSPPSGHIDVQTHLAEEFNTRLAVCGNPA---TSQPVTSS-NHSSRGGSLQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRLQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGV--KRADLLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSMNHQVIHWFWKAVWMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYE 864 HSP 2 Score: 664.455 bits (1713), Expect = 0.000e+0 Identity = 381/857 (44.46%), Postives = 503/857 (58.69%), Query Frame = 0
Query: 799 NPNSFPRAHTCFNRLDLPPY-------------EMISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVATTG---HLPDTF--YTLRKRSPKSKVRGRLFLQIENKPDSNPTNTPSSQPQTEPHATGASVAPLPRVANGIIPDAALPE---GWEERQSATGRTYYVDHGHREVHCTHPS---------------RACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTE-------AEPGGHPTIDD--PEEIE-----LGSPHTAIP-TGNTRETPSNVVEPCQSSAPPERTIP---QDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWV------DPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
NPNS H F PY ++I+G LAKKDI GASDPYV V+L +G ++ T +KKSLNP W LFR P H +LF+VFDENR+TRDDFLG V V L + T P TF + L RS KS+V+G L L++ P + + + Q E G V P A + GWEERQ GRTYYV+H R PS +A + DV+ S P + E V +TE + P GH + EE G+P T+ P T + + ++ C P T+P + GLP GW K + GR ++++HN++ TTW DPR+ P+ L G+++ D L LP GWEE+ DGR FI+H K+TQWEDPR+ A G + + +YK+KYE F ++L +P K + +RR +I +DS+ RI + ++D LKARLW+EF+GE GLD+GG+AREWFFL+SKEMFNPYYGLFEYSA D+YTLQINP SG+ + DHL+YF+FIG+VAGMAVYHG LLDGFFIRPFYKMML + ITL DMESVD EYY+SL +I ++DP L+L F +DE+ G T +L G+++ VT NK +YI VI WRFV+R+Q+QM AF+ GF IP + + +FDE ELELL+CG+ +D+ DW +HT Y+ GY NHQV+ WFW++V ++ R RLLQFVTGTSRVPMNGF L GSN ++F ++ +G+PD PR+HTCFNRLDLP Y S+ EL +L MA++N + FDGVD
Sbjct: 53 NPNSLEEEHVLFT---ADPYLELHNDDTRVVRVKVIAGIGLAKKDILGASDPYVRVTLYDPMSGILTSVQ--TKTIKKSLNPKWNEEILFRVLPQRHRILFEVFDENRLTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSEDENADQ--AEELEPGWVVLDQPDAATHLPHPPEPSPLPPGWEERQDVLGRTYYVNHESRRTQWKRPSPDDDLTDEDNDDMQLQAQRAFTTRRQISEDVDGPDNRES--PENWEIVREDENTEYSGQAVQSPPSGHIDVQTHLAEEFNTRLAVCGNPATSQPVTSSNHSSRGGSLQTCIFEEQP--TLPVLLPTSSGLPPGWEEK-QDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTDSND--------LGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRLQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRAD--LLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSMNHQVIHWFWKAVWMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD 887
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Match: gi|32469607|sp|Q62940.1|NEDD4_RAT (RecName: Full=E3 ubiquitin-protein ligase NEDD4; AltName: Full=HECT-type E3 ubiquitin transferase NEDD4) HSP 1 Score: 792.727 bits (2046), Expect = 0.000e+0 Identity = 436/853 (51.11%), Postives = 555/853 (65.06%), Query Frame = 0
Query: 2 AESRIVGYVPPPGSTDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVT-VDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPT--KQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPA----LTDNTAATAAVNENNASELSRRRVHISMD-----EQRPSLDIGRSRLGSDSVIDELHS-------LNINRGESVDTP----DGPSTSGTTTSA--AATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTE-GLPQEWTMQVAPNGRVFFINHSEKKTTWV------DPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRISTIG--GPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAT-DNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
AES I + + D T+++R+KVIAG GLAKKDI GASDPY+R+ L + G V SV TKT K++LNPKW EE +FRV P H+++ EVFDENRLTRDDFLG V++ L LP E+ P K ++L PRS +S+VKG L+L Y+ + + +E EELE GW ++ + P LP GWEERQD GRTYYVNH +R+TQW+ P+ LTD+ + + A +RR++ +D E + +I R + + S + + E +DT P+TS TS+ ++ GG+ S + TLP L T GLP W + GR ++++H+ K TTW DPR+ P+ + +G P +DLGPLP GWEER HTD R+FFI+HN + TQWEDPR+ + GPA PY+RDYKRKY++ + +LKK +++PNKFE+K+RR ILEDS+R ++ + + D LK +LWIEFD E GLDYGG+AREWF+L+SKEMFNPYYGLFEYSAT DNYTLQINP SG+CNEDH+SYFKFIGR+AGMAVYHGKLLDGFFIRPFYKMML K ITL DMESVD+EYY+SL WI ENDP EL+L F +DEE FG T ELK G+++ VTN++K +YI VIQWRFV+R+++QM FK GF EL+P L+KIFDE ELELLMCG+G +DV DW+ +T YK Y NH VI WFW+ VL ++E R RLLQFVTGTSRVPMNGF ELYGSNGPQ +T+E+WG P+ PRAHTCFNRLDLPPYE
Sbjct: 62 AESIISSF-----NNDDTRVVRVKVIAGIGLAKKDILGASDPYVRVTLYDPMSG--VLTSVQTKTIKKSLNPKWNEEILFRVLPQQHRILFEVFDENRLTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSDDENADQAEELE------PGWVVLDQPDAATHLQ--------------------HPPEPSPLPPGWEERQDVLGRTYYVNHESRTTQWKRPSPEDDLTDDENGDIQLQAHGAF-TTRRQISEDVDGPDNHESPENWEIVREDENTIYSGQAVQSPPSGHPDVQVRLAEELDTRLTMYGNPATSQPVTSSNHSSRGGS----------SQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHL--RGKTP--VDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRMQNVAITGPAEPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGV--KRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATEDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEVVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSLNHQVIHWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYE 864 HSP 2 Score: 664.455 bits (1713), Expect = 0.000e+0 Identity = 377/829 (45.48%), Postives = 497/829 (59.95%), Query Frame = 0
Query: 819 EMISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVATTG---HLPDTF--YTLRKRSPKSKVRGRLFLQIENKPDSNPTNTPSSQPQTEPHATGASVAPLPRVANGI---IPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVD--------------------------TEAEPGGHPTID-------DPEEIELGSPHTAIP-TGNTRETPSNVVEPCQSSAPPERTIP---QDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWV------DPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDD-YTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
++I+G LAKKDI GASDPYV V+L +G ++ T +KKSLNP W LFR P H +LF+VFDENR+TRDDFLG V V L + T P TF + L RS KS+V+G L L++ P + + + Q E G V P A + + LP GWEERQ GRTYYV+H R PS D GD++ Q+ T R ++ VD ++ P GHP + D G+P T+ P T + + + C P T+P + GLP GW K + GR ++++HN++ TTW DPR+ P+ L G++ P D L LP GWEE+ DGR FI+H K+TQWEDPR+ A G + +YK+KYE F ++L +P K + +RR +I +DS+ RI + ++D LKARLW+EF+GE GLD+GG+AREWFFL+SKEMFNPYYGLFEYSA +D YTLQINP SG+ + DHL+YF+FIG+VAGMAVYHG LLDGFFIRPFYKMML + ITL DMESVD EYY+SL +I ++DP L+L F +DE+ G T +L G++V VT NK +YI VI WRFV+R+Q+QM AF+ GF IP + + +FDE ELELL+CG+ +D+ DW +HT Y+ GY NHQV+ WFW++VL ++ R RLLQFVTGTSRVPMNGF L GSN ++F ++ +G+PD PR+HTCFNRLDLP Y S+ EL +L MA++N + FDGVD
Sbjct: 80 KVIAGIGLAKKDILGASDPYVRVTLYDPMSGVLTSVQ--TKTIKKSLNPKWNEEILFRVLPQQHRILFEVFDENRLTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSDDENADQ--AEELEPGWVVLDQPDAATHLQHPPEPSPLPPGWEERQDVLGRTYYVNHESRTTQWKRPSPEDDL---TDDENGDIQLQAHGAFTT---RRQISEDVDGPDNHESPENWEIVREDENTIYSGQAVQSPPSGHPDVQVRLAEELDTRLTMYGNPATSQPVTSSNHSSRGGSSQTCIFEEQP--TLPVLLPTSSGLPPGWEEK-QDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKT-----PVDSND-LGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRMQNVAITGPAEPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRAD--FLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATEDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEVVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSLNHQVIHWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD 887
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Match: gi|73921204|sp|Q96PU5.2|NED4L_HUMAN (RecName: Full=E3 ubiquitin-protein ligase NEDD4-like; AltName: Full=HECT-type E3 ubiquitin transferase NED4L; AltName: Full=NEDD4.2; AltName: Full=Nedd4-2) HSP 1 Score: 792.727 bits (2046), Expect = 0.000e+0 Identity = 446/979 (45.56%), Postives = 565/979 (57.71%), Query Frame = 0
Query: 19 TKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPT--KQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAAT----AAVNENNASELSRRRVHISMD-EQRPS---------------LDIGRSRLG-------------------SDSVIDELHSLNINRGESVDT-----PDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPL-----NTEGLPQEWTMQVAPNGRVFFINHSEKKTTW-----------------------VDPRTGRPSSV----------------------------------PSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIS-----------------------------------------------------TIGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYEMISGADLAKKDIFGASD 836
+++LR+KV++G LAKKDIFGASDPY+++ L D V TKT K+TLNPKW EEF FRV PS H+L+ EVFDENRLTRDDFLG V++ L++LP E P K +LLRPRS +S+VKG L+L AY+ + +E E + GWE+V ++ D+ EE LP GWEE+ D GRTYYVNH R+TQW P+L D ++ + +N+ A R R HIS D E PS ++I LG S+ + L + GE + P S + T A A G +P S P+G A S + GG P+P T GLP W + GR +++NH+ + TTW ++P+ RP S+ PS N P H+ LP GWE R+ + R FFIDHNT+TT WEDPR+ I GPA+PY+R++K+KYDY + +LKKP+++PN+FE+K+ R I E+S+R ++ + + DVLK +LWIEF++E GLDYGG+AREWF+LLSKEMFNPYYGLFEYSATDNYTLQINP SG+CNEDH+SYF FIGR+AG+AV+HGKLLDGFFIRPFYKMMLGK ITL DMESVD+EYYNSL WI ENDP EL+L F +DEE FG T +LKPNG++I VTNE+K +YI VIQWRFV+RV++QMN F GF EL+P+ L+KIFDE ELELLMCG+G +DV DW+ +++YK Y PNH VIQWFW+ VL + E R RLLQFVTGTSRVPMNGF ELYGSNGPQL+TIE+WG+P PRAHTCFNRLDLPPYE DL +K + +
Sbjct: 19 SRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDE-----ENSDQRDDMEHGWEVVDSN----DSASQHQEE----------------LPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLP-PPSAPAGRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSV--KRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFE--DLREKLLMAVEN 967 HSP 2 Score: 615.535 bits (1586), Expect = 0.000e+0 Identity = 324/682 (47.51%), Postives = 436/682 (63.93%), Query Frame = 0
Query: 918 DTFYTLRKRSPKSKVRG-RLFLQIENKPDSNPTNTPSSQPQTEPHATGASVAPLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQS---SAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
+ F +L +R P S++R + + + P + P+ + ++ G P P VA + LP GWEER+ A GRTYYV+H +R T P + DG +G S + P R +S T T+ P E SP ++T SN P S S P+ + Q LP GW +++APN GR FFI+HNT+ TTW DPR P + ++ S + L LP GWEE+I DGR +IDH K TQWEDPR+ A G + + +K+KY+ F K+L P +P + + + R++IF++S+ RI ++ + P+ LKARLW+EFE E GLD+GG+AREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + DHL+YF FIG+VAG+AV+HG LLDGFFIRPFYKMML ++ITL DMESVD EYYNSL +I ++DP L+L F +DE+ G T DL P G+++ VT NK +YID VI WRFV+RVQ+QM AF GF +P++ + +FDE ELELL+CG+ +D+ DW +H++Y+ GY PNH V+QWFW++VL + R RLLQFVTGTSRVPMNGF L GSN + F I+ +GSP+ PR+HTCFNRLDLP Y ++++LR +L+MAV+NA+ F+GVD
Sbjct: 324 EQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAGRARSSTVTGGEE--PTPSVAY-VHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQ----LAEDGASGSA-TNSNNHLIEPQIRRPRSLSSPTV-------TLSAPLEGAKDSPVRRA----VKDTLSNPQSPQPSPYNSPKPQHKVTQSF--LPPGWEMRIAPN-GRPFFIDHNTKTTTWEDPRLKFPVHMRSKT------SLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKR--PDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD 975 HSP 3 Score: 151.369 bits (381), Expect = 1.283e-35 Identity = 136/420 (32.38%), Postives = 193/420 (45.95%), Query Frame = 0
Query: 819 EMISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVAT---TGHLPDTF--YTLRKRSPKSKVRGRLFLQIENKPDSNPTNTPSSQPQTEPHATGASVAPLPRVANGIIPD---AALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDP-----EEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPER--TIPQDTEGLPSGWGLKVAPNNG-RVFFINHNTRETTWVDPRTNRPSPLPGRSEDF-----DDPSQEKDHLMD---LPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSN 1214
+++SG DLAKKDIFGASDPYV +SL + L T +KK+LNP W F FR NP H LLF+VFDENR+TRDDFLG V V L + T T P TF + LR RS KS+V+G L L++ P + + +S Q + G V A+ + LP GWEE+ GRTYYV+H +R PS +D N + ++ R R R HIS D E EP + +P EE+ + + +P + P + S R I D+ G ++ P++ R + E + P PS GR+ ++P+ ++ LP GWEE+ GR +++H + T W P + L+ S +SN
Sbjct: 25 KVVSGIDLAKKDIFGASDPYVKLSLYVADENRELAL-VQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSD-QRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPS-LMDVSSESDNNIRQINQEAAHRR----FRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPP----PSAPAGRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSN 433
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Match: gi|73921205|sp|Q8CFI0.2|NED4L_MOUSE (RecName: Full=E3 ubiquitin-protein ligase NEDD4-like; AltName: Full=HECT-type E3 ubiquitin transferase NED4L; AltName: Full=NEDD4.2; AltName: Full=Nedd4-2) HSP 1 Score: 788.489 bits (2035), Expect = 0.000e+0 Identity = 447/986 (45.33%), Postives = 567/986 (57.51%), Query Frame = 0
Query: 14 GSTDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPT--KQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAAT----AAVNENNASELSRRRVHISMD-EQRPSLDIGRSRLGSDSVIDELH--------------------------SLNINRGESVDTPDG------------PST---SGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPL-----NTEGLPQEWTMQVAPNGRVFFINHSEKKTTW-----------------------VDPRTGRPSSV----------------------------------PSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIS-----------------------------------------------------TIGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYEMISGADLAKKDIFGASD 836
G +++LR+KV++G LAKKDIFGASDPY+++ L D V TKT K+TLNPKW EEF FRV PS H+L+ EVFDENRLTRDDFLG V++ L++LP E P K +LLRPRS +S+VKG L+L AY+ + +E E E GWE+V ++ D+ EE LP GWEE+ D GRTYYVNH RSTQW P+L D ++ + +N+ A R R HIS D E S G +++ +E++ S ++R + TPD PS+ S + T A +P S P+ A S + GG P+P T GLP W + GR +++NH+ + TTW V+P+ RP S+ PS N P H+ LP GWE R+ + R FFIDHNT+TT WEDPR+ I GPA+PY+R++K+KYDY + +LKKP+++PN+FE+K+ R I E+S+R ++ + + DVLK +LWIEF++E GLDYGG+AREWF+LLSKEMFNPYYGLFEYSATDNYTLQINP SG+CNEDH+SYF FIGR+AG+AV+HGKLLDGFFIRPFYKMMLGK ITL DMESVD+EYYNSL WI ENDP EL+L F +DEE FG T +LKPNG++I VTNE+K +YI VIQWRFV+RV++QMN F GF EL+P+ L+KIFDE ELELLMCG+G +DV DW+ +++YK Y PNH VIQWFW+ VL + E R RLLQFVTGTSRVPMNGF ELYGSNGPQL+TIE+WG+P PRAHTCFNRLDLPPYE DL +K + +
Sbjct: 42 GGQGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDE-----ENSEQRDDMEHGWEVVDSN----DSASQHQEE----------------LPPPPLPPGWEEKVDNLGRTYYVNHNNRSTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEASEGGGEGPEPWETISEEMNMAGDSLSLALPPPPASPVSRTSPQELSEEVSRRLQI-TPDSNGEQFSSLIQREPSSRLRSCSVTDTVAEQAHLP-PPSTPTRRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLVEPQIRRPRSLSSPTVTLSAPLEGAKDSPIRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKASLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSV--KRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFE--DLREKLLMAVEN 996 HSP 2 Score: 618.231 bits (1593), Expect = 0.000e+0 Identity = 325/682 (47.65%), Postives = 437/682 (64.08%), Query Frame = 0
Query: 918 DTFYTLRKRSPKSKVRG-RLFLQIENKPDSNPTNTPSSQPQTEPHATGASVAPLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQS---SAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
+ F +L +R P S++R + + + P +TP+ + ++ G P P VA + LP GWEER+ A GRTYYV+H +R T P + DG +G S + P R +S T T+ P E SP ++T SN P S S P+ + Q LP GW +++APN GR FFI+HNT+ TTW DPR P + ++ S + L LP GWEE+I DGR +IDH K TQWEDPR+ A G + + +K+KY+ F K+L P +P + + + R++IF++S+ RI ++ + P+ LKARLW+EFE E GLD+GG+AREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + DHL+YF FIG+VAG+AV+HG LLDGFFIRPFYKMML ++ITL DMESVD EYYNSL +I ++DP L+L F +DE+ G T DL P G+++ VT NK +YID VI WRFV+RVQ+QM AF GF +P++ + +FDE ELELL+CG+ +D+ DW +H++Y+ GY PNH V+QWFW++VL + R RLLQFVTGTSRVPMNGF L GSN + F I+ +GSP+ PR+HTCFNRLDLP Y ++++LR +L+MAV+NA+ F+GVD
Sbjct: 353 EQFSSLIQREPSSRLRSCSVTDTVAEQAHLPPPSTPTRRARSSTVTGGEE--PTPSVAY-VHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQ----LAEDGASGSA-TNSNNHLVEPQIRRPRSLSSPTV-------TLSAPLEGAKDSPIRRA----VKDTLSNPQSPQPSPYNSPKPQHKVTQSF--LPPGWEMRIAPN-GRPFFIDHNTKTTTWEDPRLKFPVHMRSKA------SLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKR--PDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD 1004 HSP 3 Score: 151.369 bits (381), Expect = 1.415e-35 Identity = 139/419 (33.17%), Postives = 191/419 (45.58%), Query Frame = 0
Query: 819 EMISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVAT---TGHLPDTF--YTLRKRSPKSKVRGRLFLQIENKPDSNPTNTPSSQPQTEPHATGASVAPLPRVANGIIPD---AALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEP------GGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPER--TIPQDTEGLPSGWGLKVAPNNG-RVFFINHNTRETTWVDP-----RTNRPSPLPGRSEDFDDPSQEKDHLM-DLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSN 1214
+++SG DLAKKDIFGASDPYV +SL + L T +KK+LNP W F FR NP H LLF+VFDENR+TRDDFLG V V L + T T P TF + LR RS KS+V+G L L++ P + + +S+ Q + G V A+ + LP GWEE+ GRTYYV+H +R PS +D N + ++ R R R HIS D E E G P EE+ + ++ +P + P + S R I D+ G ++ P++ R + E + P R R S + G E PS H LP GWEE+ GR +++H + T W P + L+ S +SN
Sbjct: 53 KVVSGIDLAKKDIFGASDPYVKLSLYVADENRELAL-VQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSE-QRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRSTQWHRPS-LMDVSSESDNNIRQINQEAAHRR----FRSRRHISEDLEPEASEGGGEGPEPWETISEEMNMAGDSLSLALPPPPASPVSRTSPQELSEEVSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDTVAEQAHLPPPSTPTRRARSSTVTGGEE--PTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSN 462
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Match: gi|73921206|sp|Q5RBF2.1|NED4L_PONAB (RecName: Full=E3 ubiquitin-protein ligase NEDD4-like; AltName: Full=HECT-type E3 ubiquitin transferase NED4L) HSP 1 Score: 781.556 bits (2017), Expect = 0.000e+0 Identity = 442/980 (45.10%), Postives = 559/980 (57.04%), Query Frame = 0
Query: 13 PGSTDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPT--KQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAAT----AAVNENNASELSRRRVHISMD-EQRPS---------------LDIGRSRLG-------------------SDSVIDELHSLNINRGESVDT-----PDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTW-----------------------VDPRTGRPSSV----------------------------------PSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIS-----------------------------------------------------TIGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYEMISGADLAKKDIFGASD 836
P +++LR+KV++G LAKKDIFGASDPY+++ L D V TKT K+TLNPKW EEF FRV PS H+L+ EVFDENRLTRDDFLG V++ L++LP E P K +LLRPRS +S+VKG L+L AY+ + EE E E GWE+V ++ D+ EE LP GWEE+ D GRTYYVNH R+TQW P+L D ++ + +N+ A R R HIS D E PS ++I LG S+ + L + GE + P S + T A A G +P PS++ V T GLP W + GR +++NH+ + TTW ++P+ RP S+ PS N P H+ LP GWE R+ + R FFIDHNT+TT WEDPR+ I GPA+PY+R++K+KYDY + +LKKP+++PN+FE+K+ R I E+S+R ++ + + DVLK +LWIEF++E GLDYGG+AREWF+LLSKEMFNPYYGLFEYSATDNYTLQINP SG+CNEDH+SYF FIGR+AG+AV+HGKLLDGFFIRPFYKMMLGK ITL DMESVD+EYYNSL WI ENDP EL+L F +DEE FG T +L+PNG++I VTNE+K +YI VIQWRFV+RV++QMN F GF EL+P+ L+KIFDE ELELLMCG+G +DV DW+ +++YK Y PNH VIQWFW+ VL + E R RLLQFVTGTSRVPMNGF ELYGSNGPQL+TIE+WG+P PRAHTCFNRLDLPPYE DL +K + +
Sbjct: 17 PVCYGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEE-----ENSEQRDDMEHGWEVVDSN----DSASQHQEE----------------LPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLP---------PPSVAYVH-------TTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSV--KRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLEPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFE--DLREKLLMAVEN 951 HSP 2 Score: 654.44 bits (1687), Expect = 0.000e+0 Identity = 387/952 (40.65%), Postives = 512/952 (53.78%), Query Frame = 0
Query: 808 TCFNRLDLPPYEMISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVAT---TGHLPDTF--YTLRKRSPKSKVRGRLFLQIENKPDSNPTNTPSSQPQTEPHATGASVAPLPRVANGIIPD---AALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDP-----EEI-----------------------------ELGSPHTAIPTGN-------TRETPSNVVEPCQSS---------APPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTW-----------------------VDPRTNRP-----------SPLPGRSED--------------------FDDPS-QEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQ---------------------------------------------------TQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
C+ + +++SG DLAKKDIFGASDPYV +SL + L T +KK+LNP W F FR NP H LLF+VFDENR+TRDDFLG V V L + T T P TF + LR RS KS+V+G L L++ P + +S+ Q + G V A+ + LP GWEE+ GRTYYV+H +R PS +D N + ++ R R R HIS D E EP + +P EE+ EL P N + PS+ + C + PP T GLPSGW + GR +++NHN R TTW ++P+ RP +PL G + ++ P Q K LP GWE +I P+GR FIDH K TQWEDPR+ A G + + +K+KY+ F K+L P +P + + + R++IF++S+ RI ++ + P+ LKARLW+EFE E GLD+GG+AREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + DHL+YF FIG+VAG+AV+HG LLDGFFIRPFYKMML ++ITL DMESVD EYYNSL +I ++DP L+L F +DE+ G T DL P G+++ VT NK +YID VI WRFV+RVQ+QM AF GF +P++ + +FDE ELELL+CG+ +D+ DW +H++Y+ GY PNH V+QWFW++VL + R RLLQFVTGTSRVPMNGF L GSN + F I+ +GSP+ PR+HTCFNRLDLP Y ++++LR +L+MAV+NA+ F+GVD
Sbjct: 18 VCYGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELAL-VQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQEEENSE-QRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPS-LMDVSSESDNNIRQINQEAAHRR----FRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSVAYVHTTPGLPSGWEER-KDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKR--PDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLEPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD 959
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Match: gi|73921207|sp|Q9VVI3.2|NEDD4_DROME (RecName: Full=E3 ubiquitin-protein ligase Nedd-4; Short=DNedd4; AltName: Full=HECT-type E3 ubiquitin transferase NEDD4) HSP 1 Score: 753.436 bits (1944), Expect = 0.000e+0 Identity = 354/493 (71.81%), Postives = 406/493 (82.35%), Query Frame = 0
Query: 333 PRRTSVPSGSAPSLSSVGGTLPSPLNTEG----LPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
P S PS S P NT G LP W+MQVAPNGR FFI+H+ ++TTW+DPR GR S +P+Q R EDDLGPLPEGWEERVHTD R+F+IDHNTRTTQWEDPR+S I G A+PY+RDYK+KY+Y KS ++KP+NVPNKFEI++RR ILEDS+R + + +K D+LKTKLW+EF+ E GLDYGGLAREWFYLLSKEMFNPYYGLFEYSA DNYTLQIN SG+CNE+H+SYFKFIGRIAGMAVYHGKLLD FFIRPFYKMML K I LKDMESVDTEYYNSL+WIKENDP LELTF +DE+ FG ELKP GA I VTNE+K++YIK VI+WRFV+RV+EQM+ F GF ++PL+L+KIFDE ELELLMCGI +IDVKDW+ NT+YKGDYH NHI+IQWFWR VLSF+NEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQ++TIEKWG PN+FPRAHTCFNRLDLPPYE
Sbjct: 498 PSDISAPSTRRNSEEDNAAVPPMEQNTGGEEEPLPPRWSMQVAPNGRTFFIDHASRRTTWIDPRNGRASPMPNQ----TRRVEDDLGPLPEGWEERVHTDGRVFYIDHNTRTTQWEDPRLSNPNIAGQAVPYSRDYKQKYEYFKSHIRKPTNVPNKFEIRIRRTSILEDSYRIISSV--TKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFGSIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQWFWRAVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGTPNNFPRAHTCFNRLDLPPYE 984 HSP 2 Score: 597.045 bits (1538), Expect = 0.000e+0 Identity = 282/511 (55.19%), Postives = 370/511 (72.41%), Query Frame = 0
Query: 1085 TPSNVVEPCQSSAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
T N E + P E+ + E LP W ++VAPN GR FFI+H +R TTW+DPR R SP+P ++ + +D L LP+GWEE++ DGR +IDH + TQWEDPR++ G + + +YK+KYE F + P +P K I +RR I +DS+ I ++ ++D LK +LWVEFEGE+GLD+GGLAREWF+LLSKEMFNPYYGLFEYSA D+YTLQIN SG+ + +HL+YF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F +DED G +L P GA++ VT NK +YI VI WRFV+RV+EQM +F GF IPLN + +FDE ELELL+CGI ID++DW ++T+Y+G YH NH ++QWFWR+VLSFSN+MR+RLLQFVTGTSRVPMNGF L GSN + F I+ +G+P++FPR+HTCFNRLDLP Y Y +L+ +L+ A++ ++ F GVD
Sbjct: 506 TRRNSEEDNAAVPPMEQNTGGEEEPLPPRWSMQVAPN-GRTFFIDHASRRTTWIDPRNGRASPMPNQTR------RVEDDLGPLPEGWEERVHTDGRVFYIDHNTRTTQWEDPRLSNPNIAGQAVPYSRDYKQKYEYFKSHIRKPTNVPNKFEIRIRRTSILEDSYRIISSVTKTD--LLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFGSIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQWFWRAVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGTPNNFPRAHTCFNRLDLPPYEGYLQLKDKLIKAIEGSQGFAGVD 1007 HSP 3 Score: 258.84 bits (660), Expect = 1.062e-69 Identity = 153/301 (50.83%), Postives = 191/301 (63.46%), Query Frame = 0
Query: 22 LRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPR---SARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNENNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTS 319
LR+ V+ G LAKKDIFGASDPY+RIDL T++G+ DSVLTKTKK+TLNP W EEFIFRVKPS HKLV +VFDENRLTRDDFLGMVELTL NLP E EGR I + Y LRPR A+S++KG L++YHA++ E EP + GNS G WE V + + T + P P GG++ LPAGWEERQDANGRTYYVNH AR+TQW P + N+ ++ + ++ AS+ +RR HIS+D+ + GRS +DS+ N N TP +TS
Sbjct: 72 LRIVVLTGQSLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKKKTLNPTWNEEFIFRVKPSEHKLVFQVFDENRLTRDDFLGMVELTLVNLPTEQEGRTIGEQSYTLRPRRSVGAKSRIKGTLRIYHAFIRETREQSEPSS------GNSDGE--WEHV----EATNAGETSAQ--PHPFPTGGHDA---------LPAGWEERQDANGRTYYVNHTARTTQWDRPTVL-NSHSSQSTDDQLASDF-QRRFHISVDD----TESGRS---ADSISHNSIEDNNNAAGLAYTPKTAATS 340 HSP 4 Score: 142.124 bits (357), Expect = 9.686e-33 Identity = 108/290 (37.24%), Postives = 142/290 (48.97%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVATTGH---LPDTFYTLRKR---SPKSKVRGRL-----FLQIENKPDSNPTNTPSSQPQTEPHATGA---SVAPLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDT-----EAEPGGHPTIDDPEEIE--LGSPHTAIPTGNTRETPSN 1088
+++G LAKKDIFGASDPYV + L+ NG +T KK+LNP W F+FR P H L+FQVFDENR+TRDDFLG+V + L ++ T + + YTLR R KS+++G L F++ E + S P++ S AT A S P P G ALP GWEERQ A GRTYYV+H R P+ +QST + R HISVD A+ H +I+D +P TA + TP+N
Sbjct: 76 VLTGQSLAKKDIFGASDPYVRIDLN-TINGDINIDSVLTKTKKKTLNPTWNEEFIFRVKPSEHKLVFQVFDENRLTRDDFLGMVELTLVNLPTEQEGRTIGEQSYTLRPRRSVGAKSRIKGTLRIYHAFIR-ETREQSEPSSGNSDGEWEHVEATNAGETSAQPHPFPTGG---HDALPAGWEERQDANGRTYYVNHTARTTQWDRPTVLNSH-----------SSQSTDDQLASDFQRRFHISVDDTESGRSADSISHNSIEDNNNAAGLAYTPKTAATSSAPPNTPTN 349
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Match: gi|313104311|sp|P46934.4|NEDD4_HUMAN (RecName: Full=E3 ubiquitin-protein ligase NEDD4; AltName: Full=Cell proliferation-inducing gene 53 protein; AltName: Full=HECT-type E3 ubiquitin transferase NEDD4; AltName: Full=Neural precursor cell expressed developmentally down-regulated protein 4; Short=NEDD-4) HSP 1 Score: 688.337 bits (1775), Expect = 0.000e+0 Identity = 377/812 (46.43%), Postives = 482/812 (59.36%), Query Frame = 0
Query: 100 TRDDFLGMVELTLANLPRESEGRNIPT--KQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDT-TVEELEGNSAGSEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNENNASELSRRRVHIS---MDEQRPSLDIGRSRLGSDSVIDELHSLNINRG-------ESVDTPD--GPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVG----GTLPSPLNT-EGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRT------------------GRPSSVPSQGNMPNRPHEDDLGPLPEGW----------------------------------------------------EERVHTDARIFFIDHNTRTTQWEDPRISTIG--GPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
TRDDFLG V++ L LP E+ P K ++L PRS +S+VKG L+L Y+ + + SE+ + EELE GW ++ + ++ P+P LP GWEERQD GRTYYVNH +R TQW+ P DN EN +L +R + + E+ S+D S + + ++ ++ N+ ++D P + T + + P +S S SL + TLP L T GLP W + GR ++++H+ + TTW P +S G +P E + G LP+GW EER HTD RIF+I+HN + TQWEDPR+ + GPA+PY+RDYKRKY++ + +LKK +++PNKFE+K+RR +LEDS+R ++ + + D LK +LWIEFD E GLDYGG+AREWF+L+SKEMFNPYYGLFEYSATDNYTLQINP SG+CNEDH+SYFKFIGR+AGMAVYHGKLLDGFFIRPFYKMML K ITL DMESVD+EYYNSL WI ENDP EL+L F +DEE FG T ELK G++I VTN++K +YI VIQWRFV+R+++QM FK GF EL+P L+KIFDE ELELLMCG+G +DV DW+ +T YK Y NH VIQWFW+ VL ++E R RLLQFVTGTSRVPMNGF ELYGSNGPQ +T+E+WG P PRAHTCFNRLDLPPYE
Sbjct: 517 TRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELE------PGWVVLDQPDAACHLQQ---QQEPSP-----------------LPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDA----ENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGV--KRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYE 1296 HSP 2 Score: 575.474 bits (1482), Expect = 9.687e-179 Identity = 269/486 (55.35%), Postives = 352/486 (72.43%), Query Frame = 0
Query: 1110 LPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
LP GW ++ APN GR FFI+HNT+ TTW DPR P+ L G++ + L LP GWEE+ DGR +I+H K+TQWEDPR+ A G + + +YK+KYE F ++L +P K + +RR + +DS+ RI + ++D LKARLW+EF+GE GLD+GG+AREWFFL+SKEMFNPYYGLFEYSA D+YTLQINP SG+ + DHL+YF+FIG+VAGMAVYHG LLDGFFIRPFYKMML + ITL DMESVD EYYNSL +I ++DP L+L F +DE+ G T +L G+++ VT NK +YI VI WRFV+R+Q+QM AF+ GF IP + + +FDE ELELL+CG+ +D+ DW +HT Y+ GY NHQV+QWFW++VL ++ R RLLQFVTGTSRVPMNGF L GSN ++F ++ +G+P+ PR+HTCFNRLDLP Y S++EL +L MA++N + FDGVD
Sbjct: 842 LPKGWEVRHAPN-GRPFFIDHNTKTTTWEDPRLKIPAHLRGKTS-----LDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRAD--FLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD 1319 HSP 3 Score: 58.151 bits (139), Expect = 6.831e-7 Identity = 54/176 (30.68%), Postives = 72/176 (40.91%), Query Frame = 0
Query: 981 AALPEGWEERQSATGRTYYVDHGHREVHCTHPSRAC----HRIIPADGNTGDVEAQSTSRS----TRPFHRERVHISVDTEAE--PGGHPTIDD--PEEIELGSPHTAIPT---GNTRETPSNVVEPCQSSAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPR 1141
+ LP GWEE+Q GR+YYVDH R T P+ ++ + + G STS S T+P E+ + E P G P D + P IP G T SN + P LP GW + +GR+F+INHN + T W DPR
Sbjct: 767 SGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGP-----------------LPPGWEERTH-TDGRIFYINHNIKRTQWEDPR 924
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Match: gi|121734821|sp|Q0CCL1.1|RSP5_ASPTN (RecName: Full=Probable E3 ubiquitin-protein ligase hulA; AltName: Full=HECT ubiquitin ligase A; AltName: Full=HECT-type E3 ubiquitin transferase hulA) HSP 1 Score: 538.11 bits (1385), Expect = 8.317e-171 Identity = 322/831 (38.75%), Postives = 463/831 (55.72%), Query Frame = 0
Query: 22 LRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRV-KPSAHKLVLEVFDENRLTRDDFLGMVELTLAN-LPRESEGRNIPTKQYLLRPRSARSKVKGNLQL-YHAYVAEPNASEEPDTTVEELEGNSAGSEGWEIVG--------NSEQTVDTTVTGFEELP---------APIRGGGNEGSGDPTAGLV------LPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNENNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTG---RPSSVPSQGNMPNRPHE--DDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRISTIGGPAIP-YNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKEND-PAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
LR+ +IA GL K+D+F DP+ + TV GE+ T K+TLNP W E F RV + S + + + + FLG++ + + + + + G + T+ L+ + V G L + ++ PN ++ L+ +++ ++ + T + +P AP+ G G+ LPAGWE R+D GRTYYV+H R+T W P+ N A E N +L RR M + R G+ S N+ + TP P+G + + S+ T + T LP W + P GR +F++H+ + TTWVDPR R + GN + LGPLP GWE R+ AR++F+DHNT+TT W+DPR+ + +P Y RD++RK Y +SQ + + +KVRR I EDS+ +++ +S LK +L I+FD E GLDYGGL+RE+F+LLS EMFNP+Y LFEYSA DNYTLQINP+SG+ N +H++YFKFIGR+ G+A++H + LD FFI FYKMML K ++L+DME VD + + +L W +ND +ELTF VD+E+FG + +LKP G I VTNE+K++Y++ V +W+ V RVEEQ N F +GFNEL+P L+ +FDE ELELL+ GI IDV DWK +T Y+G Y VIQ FW++V +++ E +SRLLQF TGTSR+P+NGFK+L GS+GP+ +TIEK G+P + P++HTCFNRLDLPPY+
Sbjct: 11 LRVTIIAADGLYKRDVFRFPDPFA---VATVGGEQTH---TTSVIKKTLNPYWNEMFDLRVNEDSILAIQIFDQKKFKKKDQGFLGVINVRIGDVIDLQMGGDEMLTRD--LKKSNDNLVVHGKLIINLSTNLSTPNTNQANGLHRSNLQSSTSSGLVPQVTAPHASPGPSQLDPTASNPSLNPQRVPSTTRPSSTVAPVNGAAAPGASRTNLSSFEDSQGRLPAGWERREDNLGRTYYVDHNTRTTTWTRPSSNYNEATQRTQREANM-QLERRAHQSRM--------LPEDRTGASSP-------NLQENQQAQTP-------------------------PAGGSANAVSMMATGATTAGTGELPPGWEQRTTPEGRPYFVDHNTRTTTWVDPRRQQYIRMYGQNANGNNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQGVPQYKRDFRRKLIYFRSQ-PALRIMSGQCHVKVRRNNIFEDSYAEIMRQSASD---LKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGV-NPEHLNYFKFIGRVVGLAIFHRRFLDSFFIGAFYKMMLRKKVSLQDMEGVDEDLHRNLTWTLDNDIEGIIELTFAVDDEKFGERRTIDLKPGGRDIPVTNENKHEYVELVTEWKIVKRVEEQFNAFMSGFNELIPADLVNVFDERELELLIGGIADIDVDDWKKHTDYRG-YQEQDEVIQNFWKIVRTWDAEQKSRLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKSGDPAALPKSHTCFNRLDLPPYK 786 HSP 2 Score: 493.812 bits (1270), Expect = 3.740e-154 Identity = 307/835 (36.77%), Postives = 442/835 (52.93%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAV--TTVVKKSLNPYWLARFLFRANPD-LHNLLFQVFDENRITRDDFLGLVIVDLKDVATTGHLPDTFYT--LRKRSPKSKVRGRLFLQIE---NKPDSNPTN------------------------------------TPSSQPQTEPHAT--GASVAPLPRVANGIIPDAA-------------LPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPS---QEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPL-ELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAF 1591
+I+ L K+D+F DP+ +T+G E T+V+KK+LNPYW F R N D + + + + FLG++ V + DV D T L+K + V G+L + + + P++N N PS PQ P T ++VAP V P A+ LP GWE R+ GRTYYVDH R T PS + Q T R R H S + G + + E + +P P G + S + ++ E LP GW + P GR +F++HNTR TTWVDPR + + G++ + ++ + Q L LP GWE ++T R F+DH K T W+DPR+ S +G + ++++K F + + +++ + + VRR++IF+DS+ I + SD LK RL ++F+GE GLD+GGL+RE+FFLLS EMFNP+Y LFEYSA+D+YTLQINP SG+ +P+HLNYF+FIG+V G+A++H LD FFI FYKMML +K++L DME VD + + +L + D+D E + EL+F VD+++ G + DL P G D+ VT NK +Y++ V W+ V RV+EQ AF GF IP + + VFDE ELELL+ GI ID+ DW KHT YR GY +V+Q FW+ V ++ + ++RLLQF TGTSR+P+NGF LQGS+ F I+ G P + P+SHTCFNRLDLP Y +++ L +L +AV+ F
Sbjct: 15 IIAADGLYKRDVFRFPDPFAV---------ATVGGEQTHTTSVIKKTLNPYWNEMFDLRVNEDSILAIQIFDQKKFKKKDQGFLGVINVRIGDVIDLQMGGDEMLTRDLKKSNDNLVVHGKLIINLSTNLSTPNTNQANGLHRSNLQSSTSSGLVPQVTAPHASPGPSQLDPTASNPSLNPQRVPSTTRPSSTVAP---VNGAAAPGASRTNLSSFEDSQGRLPAGWERREDNLGRTYYVDHNTRTTTWTRPSSNYNEA-----------TQRTQREANMQLERRAHQSRMLPEDRTGASSPNLQENQQAQTP----PAGGSANAVSMMATGATTAGTGE---------LPPGWEQRTTPE-GRPYFVDHNTRTTTWVDPRRQQYIRMYGQNANGNNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQG-VPQYKRDFRRKLIYFRSQPAL-RIMSGQCHVKVRRNNIFEDSYAEIMRQSASD---LKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGV-NPEHLNYFKFIGRVVGLAIFHRRFLDSFFIGAFYKMMLRKKVSLQDMEGVDEDLHRNLTWTLDNDIEGIIELTFAVDDEKFGERRTIDLKPGGRDIPVTNENKHEYVELVTEWKIVKRVEEQFNAFMSGFNELIPADLVNVFDERELELLIGGIADIDVDDWKKHTDYR-GYQEQDEVIQNFWKIVRTWDAEQKSRLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKSGDPAALPKSHTCFNRLDLPPYKTHETLEHKLSIAVEETLGF 805
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Match: gi|3915818|sp|Q92462.2|PUB1_SCHPO (RecName: Full=E3 ubiquitin-protein ligase pub1; AltName: Full=HECT-type E3 ubiquitin transferase pub1) HSP 1 Score: 531.561 bits (1368), Expect = 7.410e-169 Identity = 325/839 (38.74%), Postives = 464/839 (55.30%), Query Frame = 0
Query: 18 RTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDF--LGMVELTLAN-LPRESEGRNIPTKQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLV----------------LPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNENNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTEG---LPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTG---RPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRISTIGGPAIP-YNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKEND-PAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYEMISGADLAKK 829
+++ +R+ ++A GL K+D+F DP+ ++TVDGE+ T K+TLNP W E F V ++ + ++VFD+ + + LG++ L + + L G + T+ L+ + + V G + + N S +T++ ++G+ ++ + + PA R G P A LP GWE R D GRTYYV+H NT +T + RP+L S G+ + ELHS + +SA T G P S S + + L S T G LP W + P GR +F++H+ + TTWVDPR R P+ + +P LGPLP GWE R+ AR++F+DHNT+TT W+DPR+ + +P Y RD++RK Y SQ +P + IKVRR I EDS+ +++ ++ LK +L I+FD E GLDYGGL+RE+F+LLS EMFNP+Y LFEYS+ DNYTLQINP+SG+ N +H++YFKFIGR+ G+A++H + +D FF+ FYKM+L K +TL+DMES+D EYY SL+WI +ND L+LTF V++ FG ++ +LKPNG I VT E+K +Y+ V WR R+EEQ N F GF+EL+P L+ +FDE ELELL+ GI ID++DWK +T Y+ Y N +I+WFW ++ ++NE +SRLLQF TGTSR+P+NGFK+L GS+GP+ +TIEK G PN P+AHTCFNRLDLPPY S DL K
Sbjct: 6 QSRRIRVTIVAADGLYKRDVFRFPDPFA---VLTVDGEQTH---TTTAIKKTLNPYWNETFEVNVTDNS-TIAIQVFDQKKFKKKGQGFLGVINLRVGDVLDLAIGGDEMLTRD--LKKSNENTVVHGKIII--------NLSTTAQSTLQVPSSAASGARTQRTSITNDPQSSQSSSVSRN-PASSRAGSPTRDNAPAASPASSEPRTFSSFEDQYGRLPPGWERRTDNLGRTYYVDH--------------NTRSTTWI--------------------RPNLS---SVAGAAAA--ELHS-------------------SASSANVTEGVQP------SSSNAARRTEASVLTSNATTAGSGELPPGWEQRYTPEGRPYFVDHNTRTTTWVDPRRQQYIRSYGGPNNATIQQQP-VSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNVPQYKRDFRRKLIYFLSQ-PALHPLPGQCHIKVRRNHIFEDSYAEIMRQSATD---LKKRLMIKFDGEDGLDYGGLSREYFFLLSHEMFNPFYCLFEYSSVDNYTLQINPHSGI-NPEHLNYFKFIGRVIGLAIFHRRFVDAFFVVSFYKMILQKKVTLQDMESMDAEYYRSLVWILDNDITGVLDLTFSVEDNCFGEVVTIDLKPNGRNIEVTEENKREYVDLVTVWRIQKRIEEQFNAFHEGFSELIPQELINVFDERELELLIGGISEIDMEDWKKHTDYRS-YSENDQIIKWFWELMDEWSNEKKSRLLQFTTGTSRIPVNGFKDLQGSDGPRKFTIEKAGEPNKLPKAHTCFNRLDLPPY--TSKKDLDHK 753 HSP 2 Score: 496.893 bits (1278), Expect = 7.482e-156 Identity = 298/813 (36.65%), Postives = 425/813 (52.28%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLK--DVATTGHLPDTFYT--LRKRSPKSKVRGRLFLQIENKPDS---------------------------------NPTNTPSSQPQTEPHATGASVAPLPRVANGIIPD-AALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKM--LPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHD-PEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAF 1591
+++ L K+D+F DP+ +++ + +T T +KK+LNPYW F D + QVFD+ + + L +++L+ DV D T L+K + + V G++ + + S NP ++ + P + + + PR + LP GWE R GRTYYVDH R P+ + SV A H S +A T + + SN ++S + LP GW + P GR +F++HNTR TTWVDPR + G + Q L LP GWE ++T R F+DH K T W+DPR+ S+ + + ++++K F L P + LP + I VRR+HIF+DS+ I + +D LK RL ++F+GE GLD+GGL+RE+FFLLS EMFNP+Y LFEYS+ D+YTLQINP SGI +P+HLNYF+FIG+V G+A++H +D FF+ FYKM+L +K+TL DMES+D EYY SLV+I D+D L+L+F V+++ G V+ DL P G ++ VTE NK +Y+D V WR R++EQ AF GF IP + VFDE ELELL+ GI ID++DW KHT YR Y N Q+++WFW + +SN+ ++RLLQF TGTSR+P+NGF LQGS+ F I+ G P+ P++HTCFNRLDLP YTS K+L +L +AV+ F
Sbjct: 14 IVAADGLYKRDVFRFPDPFAVLTVDGEQTHTT-------TAIKKTLNPYWNETFEVNVT-DNSTIAIQVFDQKKFKKKGQGFLGVINLRVGDVLDLAIGGDEMLTRDLKKSNENTVVHGKIIINLSTTAQSTLQVPSSAASGARTQRTSITNDPQSSQSSSVSRNPASSRAGSPTRDNAPAASPASSEPRTFSSFEDQYGRLPPGWERRTDNLGRTYYVDHNTRSTTWIRPNLS---------------------------------SVAGAAAAELH-----------SSASSANVTEGVQPSSSNAARRTEASVLTSNATTAGSGELPPGWEQRYTPE-GRPYFVDHNTRTTTWVDPRRQQYIRSYGGPNNATIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLP-SSLDQNVPQYKRDFRRKLIYF---LSQPALHPLPGQCHIKVRRNHIFEDSYAEIMRQSATD---LKKRLMIKFDGEDGLDYGGLSREYFFLLSHEMFNPFYCLFEYSSVDNYTLQINPHSGI-NPEHLNYFKFIGRVIGLAIFHRRFVDAFFVVSFYKMILQKKVTLQDMESMDAEYYRSLVWILDNDITGVLDLTFSVEDNCFGEVVTIDLKPNGRNIEVTEENKREYVDLVTVWRIQKRIEEQFNAFHEGFSELIPQELINVFDERELELLIGGISEIDMEDWKKHTDYRS-YSENDQIIKWFWELMDEWSNEKKSRLLQFTTGTSRIPVNGFKDLQGSDGPRKFTIEKAGEPNKLPKAHTCFNRLDLPPYTSKKDLDHKLSIAVEETIGF 764
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Match: gi|300681180|sp|A1D3C5.1|RSP5_NEOFI (RecName: Full=Probable E3 ubiquitin-protein ligase hulA; AltName: Full=HECT ubiquitin ligase A; AltName: Full=HECT-type E3 ubiquitin transferase RSP5) HSP 1 Score: 518.079 bits (1333), Expect = 3.430e-163 Identity = 281/611 (45.99%), Postives = 381/611 (62.36%), Query Frame = 0
Query: 218 LPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNENNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHE-------DDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRISTIGGPAIP-YNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKEND-PAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
LPAGWE R+D GRTYYV+H R+T W P+ N A + E N +L RR M + R G++S P+ P +S + A GSA ++S + + E LP W + P GR +F++H+ + TTWVDPR R + G N + LGPLP GWE R+ AR++F+DHNT+TT W+DPR+ + +P Y RD++RK Y +SQ + + +KVRR I EDS+ +++ +S LK +L I+FD E GLDYGGL+RE+F+LLS EMFNP+Y LFEYSA DNYTLQINP+SG+ N +H++YFKFIGR+ G+A++H + LD FFI FYKMML K ++L+DME VD + + +L W +ND LELTF VD+E+FG + +LKP G I VTNE+K +Y++ V +W+ V RVEEQ N F +GFNEL+P L+ +FDE ELELL+ GI IDV DWK +T Y+G Y + VIQ FW+VV S++ E +SRLLQF TGTSR+P+NGFK+L GS+GP+ +TIEK G+P + P++HTCFNRLDLPPY+
Sbjct: 232 LPAGWERREDNLGRTYYVDHNTRTTTWTRPSSNYNEHAQRSQREANM-QLERRAHQSRM--------LPEDRTGANS------------------PNLPESSQQAHTPPA------------GGSANAVSMMATGATTAGTGE-LPPGWEQRTTPEGRPYFVDHNTRTTTWVDPR--RQQYIRMYGQNANGTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQGVPQYKRDFRRKLIYFRSQ-PALRIMSGQCHVKVRRNNIFEDSYAEIMRQSASD---LKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGV-NPEHLNYFKFIGRVVGLAIFHRRFLDSFFIGAFYKMMLRKKVSLQDMEGVDEDLHRNLTWTLDNDIEGVLELTFSVDDEKFGERRTIDLKPGGRDIPVTNENKAEYVELVTEWKIVKRVEEQFNAFMSGFNELIPADLVNVFDERELELLIGGIADIDVDDWKKHTDYRG-YQESDEVIQNFWKVVRSWDAEQKSRLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKSGDPAALPKSHTCFNRLDLPPYK 794 HSP 2 Score: 491.886 bits (1265), Expect = 2.017e-153 Identity = 309/839 (36.83%), Postives = 442/839 (52.68%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAV--TTVVKKSLNPYWLARFLFRANPD-LHNLLFQVFDENRITRDDFLGLVIVDLKDVATTGHLPDTFYT--LRKRSPKSKVRGRLFLQIE---NKPDSNPTN-----------TPSSQPQTEP---HATGASVAPL-----------PRVANGIIP------------------------------DAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPS---QEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEP-LELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAF 1591
+I+ L K+D+F DP+ +T+G E T+V+KK+LNPYW F R N D + + + + FLG++ V + DV D T L+K + V G+L + + + P++N N + PQ P H + AP+ RV + P LP GWE R+ GRTYYVDH R T PS + AQ + R R H S + G + + PE + HT P G + S + ++ E LP GW + P GR +F++HNTR TTWVDPR + + G++ + + + Q L LP GWE ++T R F+DH K T W+DPR+ S +G + ++++K F + + +++ + + VRR++IF+DS+ I + SD LK RL ++F+GE GLD+GGL+RE+FFLLS EMFNP+Y LFEYSA+D+YTLQINP SG+ +P+HLNYF+FIG+V G+A++H LD FFI FYKMML +K++L DME VD + + +L + D+D E LEL+F VD+++ G + DL P G D+ VT NK +Y++ V W+ V RV+EQ AF GF IP + + VFDE ELELL+ GI ID+ DW KHT YR GY + +V+Q FW+ V S+ + ++RLLQF TGTSR+P+NGF LQGS+ F I+ G P + P+SHTCFNRLDLP Y SY+ L ++ +AV+ F
Sbjct: 15 IIAADGLYKRDVFRFPDPFAV---------ATVGGEQTHTTSVIKKTLNPYWNEMFDLRVNEDSILAIQIFDQKKFKKKDQGFLGVINVRIGDVIDLQMGGDEMLTRDLKKSNDNLVVHGKLIINLSTNLSTPNTNQANGLHRSHVQSSTSSGLVPQVAPSSSHPAASGAAPVDPSASNPSLNPQRVPSTTRPSSTAAPASAAGAAASNTHGSRTNLSSFEDSQGRLPAGWERREDNLGRTYYVDHNTRTTTWTRPSSNYNE-----------HAQRSQREANMQLERRAHQSRMLPEDRTGANSPNLPESSQ--QAHTP-PAGGSANAVSMMATGATTAGTGE---------LPPGWEQRTTP-EGRPYFVDHNTRTTTWVDPRRQQYIRMYGQNANGTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQG-VPQYKRDFRRKLIYFRSQPAL-RIMSGQCHVKVRRNNIFEDSYAEIMRQSASD---LKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGV-NPEHLNYFKFIGRVVGLAIFHRRFLDSFFIGAFYKMMLRKKVSLQDMEGVDEDLHRNLTWTLDNDIEGVLELTFSVDDEKFGERRTIDLKPGGRDIPVTNENKAEYVELVTEWKIVKRVEEQFNAFMSGFNELIPADLVNVFDERELELLIGGIADIDVDDWKKHTDYR-GYQESDEVIQNFWKVVRSWDAEQKSRLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKSGDPAALPKSHTCFNRLDLPPYKSYETLEHKMSIAVEETLGF 813
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Match: gi|1228006007|ref|XP_021934893.1| (E3 ubiquitin-protein ligase Nedd-4 isoform X3 [Zootermopsis nevadensis]) HSP 1 Score: 1072.77 bits (2773), Expect = 0.000e+0 Identity = 536/822 (65.21%), Postives = 630/822 (76.64%), Query Frame = 0
Query: 6 IVGYVPPPG--STDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSE-GWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNE---NNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
+ GY+ G D T LRL+VIAGH LAKKDIFGASDPY+RIDL T++G+E DS LTKTKK+TLNP+W EEFI RVKP+ HKLVL+VFDENRLTRDDFLGMVELTL NLP+E EGR++P+KQY+LRPRSARS+VKG+L++YHA+V EP + EE EE+ ++ WEIV N +++ E P+ E LP GWEERQDANGRTY+VNH+ARSTQW+ P + V E +A +RR HIS+D DI +R +D +++ + DT D PS S+P P+ V N+EGLPQ WTMQVAPNGRVFFI+H+E+ TTWVDPRTGR S +P+Q + PNR ED+LG LPEGWEERVHTD RIFFIDHNTRTTQWEDPR+S I GPA+PY+RDYKRKY+YLKSQL+KP+NVPNKFEIKVRRQ ILEDS+R + + +++++ KTKLW+EF+ E+GLDYGGLAREWF+LLSKEMFNPYYGLFEYSA DNYTLQINP+SG+CNE+H++YFKFIGRIAGMAVYHGKLLD FFIRPFYKMMLGK I LKDMESVD+EYYNSLLWIKENDP+ELELTF VDEE FG RELKP+GA I +TNE+K++YI VIQWRFVSRV++QMN F GFN LVPL+L+KIFDE ELELLMCGI +IDVKDWK NT+YKGDYHPNHI++QWFWRVVLSFNNEMR+RLLQFVTGTSRVPMNGFKELYGSNGPQL+TIEKWG P ++PRAHTCFNRLDLPPYE
Sbjct: 16 VYGYMRSVGVEDMDETSALRLRVIAGHQLAKKDIFGASDPYVRIDLNTINGDETVDSFLTKTKKKTLNPQWNEEFILRVKPAEHKLVLQVFDENRLTRDDFLGMVELTLLNLPKEQEGRSVPSKQYILRPRSARSRVKGHLEIYHAFVREP-SQEEVMRLAEEVSSTQPETDPDWEIVEN-----ESSPPNVESAAQPVFVQNQEQLTP------LPHGWEERQDANGRTYFVNHIARSTQWERPTFNNAGTCNDQVRERSLESAVTEFQRRFHISVD------DIEANRRENDPAVNQ----------ASDTTDSPSQLQHEAETP----------SMPEVRVPNTLGV--------NSEGLPQGWTMQVAPNGRVFFIDHNERATTWVDPRTGRASPMPNQNHPPNRRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQNILEDSYRIISSV--NRMELFKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKAIDLKDMESVDSEYYNSLLWIKENDPSELELTFCVDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNSFLDGFNGLVPLNLVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHIIVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGQPENYPRAHTCFNRLDLPPYE 789 HSP 2 Score: 744.577 bits (1921), Expect = 0.000e+0 Identity = 390/800 (48.75%), Postives = 512/800 (64.00%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLV---IVDLKDVATTGHLPDTFYTLRKRSPKSKVRGRL--FLQIENKPDSNPT-----NTPSSQPQTEP--HATGASVAPLPRVANGIIPD--------AALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPERTIPQ----DTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
+I+G LAKKDIFGASDPYV + L+ + T+ +T KK+LNP W F+ R P H L+ QVFDENR+TRDDFLG+V +++L +P Y LR RS +S+V+G L + +P S+QP+T+P +P P V + P LP GWEERQ A GRTY+V+H R P+ N G Q RER S TE + H ++DD E + +T ++PS + ++ + PE +P ++EGLP GW ++VAPN GRVFFI+HN R TTWVDPRT R SP+P ++ + + +D L LP+GWEE++ DGR FIDH + TQWEDPR++ G + + +YK+KYE +L P +P K I VRR +I +DS+ I ++ + + K +LWVEFEGE GLD+GGLAREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + +HLNYF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F VDE+ G +L P GA++ +T NK +YI VI WRFVSRVQ+QM +F GF +PLN + +FDE ELELL+CGI ID++DW ++T+Y+G YHPNH +VQWFWR VLSF+N+MRARLLQFVTGTSRVPMNGF L GSN + F I+ +G P+++PR+HTCFNRLDLP Y SY++LR +L+ A++ ++ F GVD
Sbjct: 38 VIAGHQLAKKDIFGASDPYVRIDLNTINGDETVD-SFLTKTKKKTLNPQWNEEFILRVKPAEHKLVLQVFDENRLTRDDFLGMVELTLLNLPKEQEGRSVPSKQYILRPRSARSRVKGHLEIYHAFVREPSQEEVMRLAEEVSSTQPETDPDWEIVENESSP-PNVESAAQPVFVQNQEQLTPLPHGWEERQDANGRTYFVNHIARSTQWERPTF---------NNAGTCNDQV---------RERSLESAVTEFQRRFHISVDDIEANRRENDPAVNQASDTTDSPSQLQHEAETPSMPEVRVPNTLGVNSEGLPQGWTMQVAPN-GRVFFIDHNERATTWVDPRTGRASPMPNQNHPPN--RRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQNILEDSYRIISSVNRME--LFKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKAIDLKDMESVDSEYYNSLLWIKENDPSELELTFCVDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNSFLDGFNGLVPLNLVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHIIVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGQPENYPRAHTCFNRLDLPPYESYQQLREKLIKAIEGSQGFAGVD 812
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Match: gi|1228006005|ref|XP_021934892.1| (E3 ubiquitin-protein ligase Nedd-4 isoform X2 [Zootermopsis nevadensis]) HSP 1 Score: 1071.61 bits (2770), Expect = 0.000e+0 Identity = 535/816 (65.56%), Postives = 626/816 (76.72%), Query Frame = 0
Query: 12 PPGSTDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSE-GWEIVGN--SEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNE---NNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
P D T LRL+VIAGH LAKKDIFGASDPY+RIDL T++G+E DS LTKTKK+TLNP+W EEFI RVKP+ HKLVL+VFDENRLTRDDFLGMVELTL NLP+E EGR++P+KQY+LRPRSARS+VKG+L++YHA+V EP + EE EE+ ++ WEIV N S V++ E+L LP GWEERQDANGRTY+VNH+ARSTQW+ P + V E +A +RR HIS+D DI +R +D +++ + DT D PS S+P P+ V N+EGLPQ WTMQVAPNGRVFFI+H+E+ TTWVDPRTGR S +P+Q + PNR ED+LG LPEGWEERVHTD RIFFIDHNTRTTQWEDPR+S I GPA+PY+RDYKRKY+YLKSQL+KP+NVPNKFEIKVRRQ ILEDS+R + + +++++ KTKLW+EF+ E+GLDYGGLAREWF+LLSKEMFNPYYGLFEYSA DNYTLQINP+SG+CNE+H++YFKFIGRIAGMAVYHGKLLD FFIRPFYKMMLGK I LKDMESVD+EYYNSLLWIKENDP+ELELTF VDEE FG RELKP+GA I +TNE+K++YI VIQWRFVSRV++QMN F GFN LVPL+L+KIFDE ELELLMCGI +IDVKDWK NT+YKGDYHPNHI++QWFWRVVLSFNNEMR+RLLQFVTGTSRVPMNGFKELYGSNGPQL+TIEKWG P ++PRAHTCFNRLDLPPYE
Sbjct: 44 PLEMMDETSALRLRVIAGHQLAKKDIFGASDPYVRIDLNTINGDETVDSFLTKTKKKTLNPQWNEEFILRVKPAEHKLVLQVFDENRLTRDDFLGMVELTLLNLPKEQEGRSVPSKQYILRPRSARSRVKGHLEIYHAFVREP-SQEEVMRLAEEVSSTQPETDPDWEIVENESSPPNVESAAQNQEQLTP------------------LPHGWEERQDANGRTYFVNHIARSTQWERPTFNNAGTCNDQVRERSLESAVTEFQRRFHISVD------DIEANRRENDPAVNQ----------ASDTTDSPSQLQHEAETP----------SMPEVRVPNTLGV--------NSEGLPQGWTMQVAPNGRVFFIDHNERATTWVDPRTGRASPMPNQNHPPNRRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQNILEDSYRIISSV--NRMELFKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKAIDLKDMESVDSEYYNSLLWIKENDPSELELTFCVDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNSFLDGFNGLVPLNLVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHIIVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGQPENYPRAHTCFNRLDLPPYE 804 HSP 2 Score: 744.962 bits (1922), Expect = 0.000e+0 Identity = 388/797 (48.68%), Postives = 513/797 (64.37%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLV---IVDLKDVATTGHLPDTFYTLRKRSPKSKVRGRL--FLQIENKPDSNPT-----NTPSSQPQTEP-------HATGASVAPLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPERTIPQ----DTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
+I+G LAKKDIFGASDPYV + L+ + T+ +T KK+LNP W F+ R P H L+ QVFDENR+TRDDFLG+V +++L +P Y LR RS +S+V+G L + +P S+QP+T+P ++ +V + + P LP GWEERQ A GRTY+V+H R P+ N G Q RER S TE + H ++DD E + +T ++PS + ++ + PE +P ++EGLP GW ++VAPN GRVFFI+HN R TTWVDPRT R SP+P ++ + + +D L LP+GWEE++ DGR FIDH + TQWEDPR++ G + + +YK+KYE +L P +P K I VRR +I +DS+ I ++ + + K +LWVEFEGE GLD+GGLAREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + +HLNYF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F VDE+ G +L P GA++ +T NK +YI VI WRFVSRVQ+QM +F GF +PLN + +FDE ELELL+CGI ID++DW ++T+Y+G YHPNH +VQWFWR VLSF+N+MRARLLQFVTGTSRVPMNGF L GSN + F I+ +G P+++PR+HTCFNRLDLP Y SY++LR +L+ A++ ++ F GVD
Sbjct: 58 VIAGHQLAKKDIFGASDPYVRIDLNTINGDETVD-SFLTKTKKKTLNPQWNEEFILRVKPAEHKLVLQVFDENRLTRDDFLGMVELTLLNLPKEQEGRSVPSKQYILRPRSARSRVKGHLEIYHAFVREPSQEEVMRLAEEVSSTQPETDPDWEIVENESSPPNVESAAQNQEQLTP---LPHGWEERQDANGRTYFVNHIARSTQWERPTF---------NNAGTCNDQV---------RERSLESAVTEFQRRFHISVDDIEANRRENDPAVNQASDTTDSPSQLQHEAETPSMPEVRVPNTLGVNSEGLPQGWTMQVAPN-GRVFFIDHNERATTWVDPRTGRASPMPNQNHPPN--RRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQNILEDSYRIISSVNRME--LFKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKAIDLKDMESVDSEYYNSLLWIKENDPSELELTFCVDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNSFLDGFNGLVPLNLVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHIIVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGQPENYPRAHTCFNRLDLPPYESYQQLREKLIKAIEGSQGFAGVD 827
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Match: gi|1339056813|ref|XP_023716889.1| (E3 ubiquitin-protein ligase Nedd-4 isoform X3 [Cryptotermes secundus]) HSP 1 Score: 1071.23 bits (2769), Expect = 0.000e+0 Identity = 543/831 (65.34%), Postives = 634/831 (76.29%), Query Frame = 0
Query: 1 MAESRIVGYVPPPG---STDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEE-LEGNSAGSEGWEIVGNSEQ-TVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNE---NNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPL--NTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
MA GY+P G + T LRL+VIAGH LAKKDIFGASDPY+RIDL T++G+ DSVLTKTKK+TLNP+W+EEFIFRVKP+ HKLVL+VFDENRLTRDDFLGMVEL L NLP+E EGR IP+KQY+LRPRSARS+VKG+L+LYHA++ EP E E+ L WEIV V++ E+LP LP GWEERQDANGRTYYVNH+ARSTQW+ P+ + V E +A +RR HIS+D DI +R S+S + H +N + D G T AA + T+ A +LS+ +P+ L N+EGLPQ WTMQVAPNGRVFFI+H+E+ TTWVDPRTGR S +P+Q + PNR ED+LG LPEGWEERVHTD RIFFIDHNTRTTQWEDPR+S I GPA+PY+RDYKRKY+YLKSQL+KP+NVPNKFEIKVRRQ ILEDS+R + + ++ ++LKTKLW+EF+ E+GLDYGGLAREWF+LLSKEMFNPYYGLFEYSA DNYTLQINP+SG+CNE+H++YFKFIGRIAGMAVYHGKLLD FFIRPFYKMMLGK I LKDMESVD+EYYNSLLWIKENDP+ELELTF +DEE FG RELKP+GA I +TNE+K++YI VIQWRFVSRV++QMN F GFN LVPL+ +KIFDE ELELLMCGI +IDVKDWK NT+YKGDYHPNHI +QWFWRVVLSFNNEMR+RLLQFVTGTSRVPMNGFKELYGSNGPQL+TIEKWG P ++PRAHTCFNRLDLPPYE
Sbjct: 11 MAGRNEYGYMPSVGIEHMDETTSALRLRVIAGHELAKKDIFGASDPYVRIDLNTINGDVTVDSVLTKTKKKTLNPEWDEEFIFRVKPTEHKLVLQVFDENRLTRDDFLGMVELNLLNLPKEQEGRTIPSKQYILRPRSARSRVKGHLELYHAFIREPLGGGEVMRPGEDVLRSRQESDPDWEIVDEPVMPNVESAAQNQEQLPP------------------LPHGWEERQDANGRTYYVNHIARSTQWERPSFNNGGTYNDQVRERSLESAVTEFQRRFHISVD------DIEANRRESNSAGHQEHIINGS------DEDYEFLGGETREAAD----ITDSTAQSQHEAETLSTPESRVPNSLDVNSEGLPQGWTMQVAPNGRVFFIDHNERATTWVDPRTGRASPMPNQNHPPNRRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQSILEDSYRIISSV--NRTELLKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFCLDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNAFLDGFNGLVPLNCVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHITVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGLPENYPRAHTCFNRLDLPPYE 805 HSP 2 Score: 741.11 bits (1912), Expect = 0.000e+0 Identity = 402/809 (49.69%), Postives = 513/809 (63.41%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVATTGH---LPDTFYTLRKRSPKSKVRGRLFLQ---IENKPDSNPTNTPS-----SQPQTEPHATGASVAPLPRVANGIIPDAALP---EGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGD-VEAQSTSRSTRPFHRERVHISVDT------EAEPGGHPTIDDPEEIELGSPHT-AIPTGNTRETPSNVVEPCQSS------APPERTIPQ----DTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQE-KDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
+I+G +LAKKDIFGASDPYV + L+ NG +T KK+LNP W F+FR P H L+ QVFDENR+TRDDFLG+V ++L ++ +P Y LR RS +S+V+G L L I P S+ +++P +P V + LP GWEERQ A GRTYYV+H R PS G D V +S + F R R HISVD E+ GH E I GS G TRE QS + PE +P ++EGLP GW ++VAPN GRVFFI+HN R TTWVDPRT R SP+P ++ P++ +D L LP+GWEE++ DGR FIDH + TQWEDPR++ G + + +YK+KYE +L P +P K I VRR I +DS+ I ++ +++ LK +LWVEFEGE GLD+GGLAREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + +HLNYF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F +DE+ G +L P GA++ +T NK +YI VI WRFVSRVQ+QM AF GF +PLN + +FDE ELELL+CGI ID++DW ++T+Y+G YHPNH VQWFWR VLSF+N+MRARLLQFVTGTSRVPMNGF L GSN + F I+ +G P+++PR+HTCFNRLDLP Y SY++LR +L+ A++ ++ F GVD
Sbjct: 39 VIAGHELAKKDIFGASDPYVRIDLN-TINGDVTVDSVLTKTKKKTLNPEWDEEFIFRVKPTEHKLVLQVFDENRLTRDDFLGMVELNLLNLPKEQEGRTIPSKQYILRPRSARSRVKGHLELYHAFIREPLGGGEVMRPGEDVLRSRQESDPDWEIVDEPVMPNVESAAQNQEQLPPLPHGWEERQDANGRTYYVNHIARSTQWERPS------FNNGGTYNDQVRERSLESAVTEFQR-RFHISVDDIEANRRESNSAGHQ-----EHIINGSDEDYEFLGGETREAADITDSTAQSQHEAETLSTPESRVPNSLDVNSEGLPQGWTMQVAPN-GRVFFIDHNERATTWVDPRTGRASPMPNQNHP---PNRRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQSILEDSYRIISSVNRTE--LLKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFCLDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNAFLDGFNGLVPLNCVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHITVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGLPENYPRAHTCFNRLDLPPYESYQQLRDKLVKAIEGSQGFAGVD 828
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Match: gi|1228006003|ref|XP_021934891.1| (E3 ubiquitin-protein ligase Nedd-4 isoform X1 [Zootermopsis nevadensis] >gi|646701548|gb|KDR11217.1| E3 ubiquitin-protein ligase Nedd-4 [Zootermopsis nevadensis]) HSP 1 Score: 1070.84 bits (2768), Expect = 0.000e+0 Identity = 534/814 (65.60%), Postives = 626/814 (76.90%), Query Frame = 0
Query: 12 PPGSTDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSE-GWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNE---NNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
P D T LRL+VIAGH LAKKDIFGASDPY+RIDL T++G+E DS LTKTKK+TLNP+W EEFI RVKP+ HKLVL+VFDENRLTRDDFLGMVELTL NLP+E EGR++P+KQY+LRPRSARS+VKG+L++YHA+V EP + EE EE+ ++ WEIV N +++ E P+ E LP GWEERQDANGRTY+VNH+ARSTQW+ P + V E +A +RR HIS+D DI +R +D +++ + DT D PS S+P P+ V N+EGLPQ WTMQVAPNGRVFFI+H+E+ TTWVDPRTGR S +P+Q + PNR ED+LG LPEGWEERVHTD RIFFIDHNTRTTQWEDPR+S I GPA+PY+RDYKRKY+YLKSQL+KP+NVPNKFEIKVRRQ ILEDS+R + + +++++ KTKLW+EF+ E+GLDYGGLAREWF+LLSKEMFNPYYGLFEYSA DNYTLQINP+SG+CNE+H++YFKFIGRIAGMAVYHGKLLD FFIRPFYKMMLGK I LKDMESVD+EYYNSLLWIKENDP+ELELTF VDEE FG RELKP+GA I +TNE+K++YI VIQWRFVSRV++QMN F GFN LVPL+L+KIFDE ELELLMCGI +IDVKDWK NT+YKGDYHPNHI++QWFWRVVLSFNNEMR+RLLQFVTGTSRVPMNGFKELYGSNGPQL+TIEKWG P ++PRAHTCFNRLDLPPYE
Sbjct: 44 PLEMMDETSALRLRVIAGHQLAKKDIFGASDPYVRIDLNTINGDETVDSFLTKTKKKTLNPQWNEEFILRVKPAEHKLVLQVFDENRLTRDDFLGMVELTLLNLPKEQEGRSVPSKQYILRPRSARSRVKGHLEIYHAFVREP-SQEEVMRLAEEVSSTQPETDPDWEIVEN-----ESSPPNVESAAQPVFVQNQEQLTP------LPHGWEERQDANGRTYFVNHIARSTQWERPTFNNAGTCNDQVRERSLESAVTEFQRRFHISVD------DIEANRRENDPAVNQ----------ASDTTDSPSQLQHEAETP----------SMPEVRVPNTLGV--------NSEGLPQGWTMQVAPNGRVFFIDHNERATTWVDPRTGRASPMPNQNHPPNRRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQNILEDSYRIISSV--NRMELFKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKAIDLKDMESVDSEYYNSLLWIKENDPSELELTFCVDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNSFLDGFNGLVPLNLVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHIIVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGQPENYPRAHTCFNRLDLPPYE 809 HSP 2 Score: 743.036 bits (1917), Expect = 0.000e+0 Identity = 388/799 (48.56%), Postives = 508/799 (63.58%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLV---IVDLKDVATTGHLPDTFYTLRKRSPKSKVRGRL--FLQIENKPDSNPT-----NTPSSQPQTEP---------HATGASVAPLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPERTIPQ----DTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
+I+G LAKKDIFGASDPYV + L+ + T+ +T KK+LNP W F+ R P H L+ QVFDENR+TRDDFLG+V +++L +P Y LR RS +S+V+G L + +P S+QP+T+P A P LP GWEERQ A GRTY+V+H R P+ N G Q RER S TE + H ++DD E + +T ++PS + ++ + PE +P ++EGLP GW ++VAPN GRVFFI+HN R TTWVDPRT R SP+P ++ + + +D L LP+GWEE++ DGR FIDH + TQWEDPR++ G + + +YK+KYE +L P +P K I VRR +I +DS+ I ++ + + K +LWVEFEGE GLD+GGLAREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + +HLNYF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F VDE+ G +L P GA++ +T NK +YI VI WRFVSRVQ+QM +F GF +PLN + +FDE ELELL+CGI ID++DW ++T+Y+G YHPNH +VQWFWR VLSF+N+MRARLLQFVTGTSRVPMNGF L GSN + F I+ +G P+++PR+HTCFNRLDLP Y SY++LR +L+ A++ ++ F GVD
Sbjct: 58 VIAGHQLAKKDIFGASDPYVRIDLNTINGDETVD-SFLTKTKKKTLNPQWNEEFILRVKPAEHKLVLQVFDENRLTRDDFLGMVELTLLNLPKEQEGRSVPSKQYILRPRSARSRVKGHLEIYHAFVREPSQEEVMRLAEEVSSTQPETDPDWEIVENESSPPNVESAAQPVFVQNQEQLTPLPHGWEERQDANGRTYFVNHIARSTQWERPTF---------NNAGTCNDQV---------RERSLESAVTEFQRRFHISVDDIEANRRENDPAVNQASDTTDSPSQLQHEAETPSMPEVRVPNTLGVNSEGLPQGWTMQVAPN-GRVFFIDHNERATTWVDPRTGRASPMPNQNHPPN--RRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQNILEDSYRIISSVNRME--LFKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKAIDLKDMESVDSEYYNSLLWIKENDPSELELTFCVDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNSFLDGFNGLVPLNLVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHIIVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGQPENYPRAHTCFNRLDLPPYESYQQLREKLIKAIEGSQGFAGVD 832
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Match: gi|1339056809|ref|XP_023716886.1| (E3 ubiquitin-protein ligase Nedd-4 isoform X1 [Cryptotermes secundus]) HSP 1 Score: 1068.14 bits (2761), Expect = 0.000e+0 Identity = 539/820 (65.73%), Postives = 629/820 (76.71%), Query Frame = 0
Query: 9 YVPPPGSTDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEE-LEGNSAGSEGWEIVGNSEQ-TVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNE---NNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPL--NTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
Y P + T LRL+VIAGH LAKKDIFGASDPY+RIDL T++G+ DSVLTKTKK+TLNP+W+EEFIFRVKP+ HKLVL+VFDENRLTRDDFLGMVEL L NLP+E EGR IP+KQY+LRPRSARS+VKG+L+LYHA++ EP E E+ L WEIV V++ E+LP LP GWEERQDANGRTYYVNH+ARSTQW+ P+ + V E +A +RR HIS+D DI +R S+S + H +N + D G T AA + T+ A +LS+ +P+ L N+EGLPQ WTMQVAPNGRVFFI+H+E+ TTWVDPRTGR S +P+Q + PNR ED+LG LPEGWEERVHTD RIFFIDHNTRTTQWEDPR+S I GPA+PY+RDYKRKY+YLKSQL+KP+NVPNKFEIKVRRQ ILEDS+R + + ++ ++LKTKLW+EF+ E+GLDYGGLAREWF+LLSKEMFNPYYGLFEYSA DNYTLQINP+SG+CNE+H++YFKFIGRIAGMAVYHGKLLD FFIRPFYKMMLGK I LKDMESVD+EYYNSLLWIKENDP+ELELTF +DEE FG RELKP+GA I +TNE+K++YI VIQWRFVSRV++QMN F GFN LVPL+ +KIFDE ELELLMCGI +IDVKDWK NT+YKGDYHPNHI +QWFWRVVLSFNNEMR+RLLQFVTGTSRVPMNGFKELYGSNGPQL+TIEKWG P ++PRAHTCFNRLDLPPYE
Sbjct: 42 YGPLETMDETTSALRLRVIAGHELAKKDIFGASDPYVRIDLNTINGDVTVDSVLTKTKKKTLNPEWDEEFIFRVKPTEHKLVLQVFDENRLTRDDFLGMVELNLLNLPKEQEGRTIPSKQYILRPRSARSRVKGHLELYHAFIREPLGGGEVMRPGEDVLRSRQESDPDWEIVDEPVMPNVESAAQNQEQLPP------------------LPHGWEERQDANGRTYYVNHIARSTQWERPSFNNGGTYNDQVRERSLESAVTEFQRRFHISVD------DIEANRRESNSAGHQEHIINGS------DEDYEFLGGETREAAD----ITDSTAQSQHEAETLSTPESRVPNSLDVNSEGLPQGWTMQVAPNGRVFFIDHNERATTWVDPRTGRASPMPNQNHPPNRRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQSILEDSYRIISSV--NRTELLKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFCLDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNAFLDGFNGLVPLNCVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHITVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGLPENYPRAHTCFNRLDLPPYE 825 HSP 2 Score: 740.725 bits (1911), Expect = 0.000e+0 Identity = 401/808 (49.63%), Postives = 512/808 (63.37%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVATTGH---LPDTFYTLRKRSPKSKVRGRLFLQ---IENKPDSNPTNTPS-----SQPQTEPHATGASVAPLPRVANGIIPDAALP---EGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGD-VEAQSTSRSTRPFHRERVHISVDT------EAEPGGHPTIDDPEEIELGSPHT-AIPTGNTRETPSNVVEPCQSS------APPERTIPQ----DTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
+I+G +LAKKDIFGASDPYV + L+ NG +T KK+LNP W F+FR P H L+ QVFDENR+TRDDFLG+V ++L ++ +P Y LR RS +S+V+G L L I P S+ +++P +P V + LP GWEERQ A GRTYYV+H R PS G D V +S + F R R HISVD E+ GH E I GS G TRE QS + PE +P ++EGLP GW ++VAPN GRVFFI+HN R TTWVDPRT R SP+P ++ + + +D L LP+GWEE++ DGR FIDH + TQWEDPR++ G + + +YK+KYE +L P +P K I VRR I +DS+ I ++ +++ LK +LWVEFEGE GLD+GGLAREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + +HLNYF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F +DE+ G +L P GA++ +T NK +YI VI WRFVSRVQ+QM AF GF +PLN + +FDE ELELL+CGI ID++DW ++T+Y+G YHPNH VQWFWR VLSF+N+MRARLLQFVTGTSRVPMNGF L GSN + F I+ +G P+++PR+HTCFNRLDLP Y SY++LR +L+ A++ ++ F GVD
Sbjct: 59 VIAGHELAKKDIFGASDPYVRIDLN-TINGDVTVDSVLTKTKKKTLNPEWDEEFIFRVKPTEHKLVLQVFDENRLTRDDFLGMVELNLLNLPKEQEGRTIPSKQYILRPRSARSRVKGHLELYHAFIREPLGGGEVMRPGEDVLRSRQESDPDWEIVDEPVMPNVESAAQNQEQLPPLPHGWEERQDANGRTYYVNHIARSTQWERPS------FNNGGTYNDQVRERSLESAVTEFQR-RFHISVDDIEANRRESNSAGHQ-----EHIINGSDEDYEFLGGETREAADITDSTAQSQHEAETLSTPESRVPNSLDVNSEGLPQGWTMQVAPN-GRVFFIDHNERATTWVDPRTGRASPMPNQNHPPN--RRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQSILEDSYRIISSVNRTE--LLKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFCLDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNAFLDGFNGLVPLNCVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHITVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGLPENYPRAHTCFNRLDLPPYESYQQLRDKLVKAIEGSQGFAGVD 848
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Match: gi|1330894860|gb|PNF24122.1| (E3 ubiquitin-protein ligase Nedd-4 [Cryptotermes secundus]) HSP 1 Score: 1065.06 bits (2753), Expect = 0.000e+0 Identity = 537/831 (64.62%), Postives = 625/831 (75.21%), Query Frame = 0
Query: 1 MAESRIVGYVPPPG---STDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEE-LEGNSAGSEGWEIVGNSEQ-TVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNE---NNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPL--NTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
MA GY+P G + T LRL+VIAGH LAKKDIFGASDPY+RIDL T++G+ DSVLTKTKK+TLNP+W+EEFIFRVKP+ HKLVL+VFDENRLTRDDFLGMVEL L NLP+E EGR IP+KQY+LRPRSARS+VKG+L+LYHA++ EP E E+ L WEIV V++ E+LP LP GWEERQDANGRTYYVNH+ARSTQW+ P+ + V E +A +RR HIS+D+ + NR ES SA + T+ A +LS+ +P+ L N+EGLPQ WTMQVAPNGRVFFI+H+E+ TTWVDPRTGR S +P+Q + PNR ED+LG LPEGWEERVHTD RIFFIDHNTRTTQWEDPR+S I GPA+PY+RDYKRKY+YLKSQL+KP+NVPNKFEIKVRRQ ILEDS+R + + ++ ++LKTKLW+EF+ E+GLDYGGLAREWF+LLSKEMFNPYYGLFEYSA DNYTLQINP+SG+CNE+H++YFKFIGRIAGMAVYHGKLLD FFIRPFYKMMLGK I LKDMESVD+EYYNSLLWIKENDP+ELELTF +DEE FG RELKP+GA I +TNE+K++YI VIQWRFVSRV++QMN F GFN LVPL+ +KIFDE ELELLMCGI +IDVKDWK NT+YKGDYHPNHI +QWFWRVVLSFNNEMR+RLLQFVTGTSRVPMNGFKELYGSNGPQL+TIEKWG P ++PRAHTCFNRLDLPPYE
Sbjct: 11 MAGRNEYGYMPSVGIEHMDETTSALRLRVIAGHELAKKDIFGASDPYVRIDLNTINGDVTVDSVLTKTKKKTLNPEWDEEFIFRVKPTEHKLVLQVFDENRLTRDDFLGMVELNLLNLPKEQEGRTIPSKQYILRPRSARSRVKGHLELYHAFIREPLGGGEVMRPGEDVLRSRQESDPDWEIVDEPVMPNVESAAQNQEQLPP------------------LPHGWEERQDANGRTYYVNHIARSTQWERPSFNNGGTYNDQVRERSLESAVTEFQRRFHISVDD-----------------------IEANRRES-------------NSAGHQAADITDSTAQSQHEAETLSTPESRVPNSLDVNSEGLPQGWTMQVAPNGRVFFIDHNERATTWVDPRTGRASPMPNQNHPPNRRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQSILEDSYRIISSV--NRTELLKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFCLDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNAFLDGFNGLVPLNCVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHITVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGLPENYPRAHTCFNRLDLPPYE 785 HSP 2 Score: 739.184 bits (1907), Expect = 0.000e+0 Identity = 390/794 (49.12%), Postives = 507/794 (63.85%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVATTGH---LPDTFYTLRKRSPKSKVRGRLFLQ---IENKPDSNPTNTPS-----SQPQTEPHATGASVAPLPRVANGIIPDAALP---EGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPERTIPQ----DTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
+I+G +LAKKDIFGASDPYV + L+ NG +T KK+LNP W F+FR P H L+ QVFDENR+TRDDFLG+V ++L ++ +P Y LR RS +S+V+G L L I P S+ +++P +P V + LP GWEERQ A GRTYYV+H R PS N G Q RER S TE + H ++DD E S + ++ + ++ + PE +P ++EGLP GW ++VAPN GRVFFI+HN R TTWVDPRT R SP+P ++ + + +D L LP+GWEE++ DGR FIDH + TQWEDPR++ G + + +YK+KYE +L P +P K I VRR I +DS+ I ++ +++ LK +LWVEFEGE GLD+GGLAREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + +HLNYF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F +DE+ G +L P GA++ +T NK +YI VI WRFVSRVQ+QM AF GF +PLN + +FDE ELELL+CGI ID++DW ++T+Y+G YHPNH VQWFWR VLSF+N+MRARLLQFVTGTSRVPMNGF L GSN + F I+ +G P+++PR+HTCFNRLDLP Y SY++LR +L+ A++ ++ F GVD
Sbjct: 39 VIAGHELAKKDIFGASDPYVRIDLN-TINGDVTVDSVLTKTKKKTLNPEWDEEFIFRVKPTEHKLVLQVFDENRLTRDDFLGMVELNLLNLPKEQEGRTIPSKQYILRPRSARSRVKGHLELYHAFIREPLGGGEVMRPGEDVLRSRQESDPDWEIVDEPVMPNVESAAQNQEQLPPLPHGWEERQDANGRTYYVNHIARSTQWERPSF---------NNGGTYNDQV---------RERSLESAVTEFQRRFHISVDDIEANRRESNSAGHQAADITDSTAQSQHEAETLSTPESRVPNSLDVNSEGLPQGWTMQVAPN-GRVFFIDHNERATTWVDPRTGRASPMPNQNHPPN--RRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQSILEDSYRIISSVNRTE--LLKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFCLDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNAFLDGFNGLVPLNCVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHITVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGLPENYPRAHTCFNRLDLPPYESYQQLRDKLVKAIEGSQGFAGVD 808
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Match: gi|1339056811|ref|XP_023716887.1| (E3 ubiquitin-protein ligase Nedd-4 isoform X2 [Cryptotermes secundus] >gi|1330894861|gb|PNF24123.1| E3 ubiquitin-protein ligase Nedd-4 [Cryptotermes secundus] >gi|1330894862|gb|PNF24124.1| E3 ubiquitin-protein ligase Nedd-4 [Cryptotermes secundus]) HSP 1 Score: 1061.6 bits (2744), Expect = 0.000e+0 Identity = 533/820 (65.00%), Postives = 620/820 (75.61%), Query Frame = 0
Query: 9 YVPPPGSTDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEE-LEGNSAGSEGWEIVGNSEQ-TVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNE---NNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPL--NTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
Y P + T LRL+VIAGH LAKKDIFGASDPY+RIDL T++G+ DSVLTKTKK+TLNP+W+EEFIFRVKP+ HKLVL+VFDENRLTRDDFLGMVEL L NLP+E EGR IP+KQY+LRPRSARS+VKG+L+LYHA++ EP E E+ L WEIV V++ E+LP LP GWEERQDANGRTYYVNH+ARSTQW+ P+ + V E +A +RR HIS+D+ + NR ES SA + T+ A +LS+ +P+ L N+EGLPQ WTMQVAPNGRVFFI+H+E+ TTWVDPRTGR S +P+Q + PNR ED+LG LPEGWEERVHTD RIFFIDHNTRTTQWEDPR+S I GPA+PY+RDYKRKY+YLKSQL+KP+NVPNKFEIKVRRQ ILEDS+R + + ++ ++LKTKLW+EF+ E+GLDYGGLAREWF+LLSKEMFNPYYGLFEYSA DNYTLQINP+SG+CNE+H++YFKFIGRIAGMAVYHGKLLD FFIRPFYKMMLGK I LKDMESVD+EYYNSLLWIKENDP+ELELTF +DEE FG RELKP+GA I +TNE+K++YI VIQWRFVSRV++QMN F GFN LVPL+ +KIFDE ELELLMCGI +IDVKDWK NT+YKGDYHPNHI +QWFWRVVLSFNNEMR+RLLQFVTGTSRVPMNGFKELYGSNGPQL+TIEKWG P ++PRAHTCFNRLDLPPYE
Sbjct: 42 YGPLETMDETTSALRLRVIAGHELAKKDIFGASDPYVRIDLNTINGDVTVDSVLTKTKKKTLNPEWDEEFIFRVKPTEHKLVLQVFDENRLTRDDFLGMVELNLLNLPKEQEGRTIPSKQYILRPRSARSRVKGHLELYHAFIREPLGGGEVMRPGEDVLRSRQESDPDWEIVDEPVMPNVESAAQNQEQLPP------------------LPHGWEERQDANGRTYYVNHIARSTQWERPSFNNGGTYNDQVRERSLESAVTEFQRRFHISVDD-----------------------IEANRRES-------------NSAGHQAADITDSTAQSQHEAETLSTPESRVPNSLDVNSEGLPQGWTMQVAPNGRVFFIDHNERATTWVDPRTGRASPMPNQNHPPNRRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQSILEDSYRIISSV--NRTELLKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFCLDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNAFLDGFNGLVPLNCVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHITVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGLPENYPRAHTCFNRLDLPPYE 805 HSP 2 Score: 738.799 bits (1906), Expect = 0.000e+0 Identity = 390/794 (49.12%), Postives = 507/794 (63.85%), Query Frame = 0
Query: 820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVATTGH---LPDTFYTLRKRSPKSKVRGRLFLQ---IENKPDSNPTNTPS-----SQPQTEPHATGASVAPLPRVANGIIPDAALP---EGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTAIPTGNTRETPSNVVEPCQSSAPPERTIPQ----DTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
+I+G +LAKKDIFGASDPYV + L+ NG +T KK+LNP W F+FR P H L+ QVFDENR+TRDDFLG+V ++L ++ +P Y LR RS +S+V+G L L I P S+ +++P +P V + LP GWEERQ A GRTYYV+H R PS N G Q RER S TE + H ++DD E S + ++ + ++ + PE +P ++EGLP GW ++VAPN GRVFFI+HN R TTWVDPRT R SP+P ++ + + +D L LP+GWEE++ DGR FIDH + TQWEDPR++ G + + +YK+KYE +L P +P K I VRR I +DS+ I ++ +++ LK +LWVEFEGE GLD+GGLAREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + +HLNYF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F +DE+ G +L P GA++ +T NK +YI VI WRFVSRVQ+QM AF GF +PLN + +FDE ELELL+CGI ID++DW ++T+Y+G YHPNH VQWFWR VLSF+N+MRARLLQFVTGTSRVPMNGF L GSN + F I+ +G P+++PR+HTCFNRLDLP Y SY++LR +L+ A++ ++ F GVD
Sbjct: 59 VIAGHELAKKDIFGASDPYVRIDLN-TINGDVTVDSVLTKTKKKTLNPEWDEEFIFRVKPTEHKLVLQVFDENRLTRDDFLGMVELNLLNLPKEQEGRTIPSKQYILRPRSARSRVKGHLELYHAFIREPLGGGEVMRPGEDVLRSRQESDPDWEIVDEPVMPNVESAAQNQEQLPPLPHGWEERQDANGRTYYVNHIARSTQWERPSF---------NNGGTYNDQV---------RERSLESAVTEFQRRFHISVDDIEANRRESNSAGHQAADITDSTAQSQHEAETLSTPESRVPNSLDVNSEGLPQGWTMQVAPN-GRVFFIDHNERATTWVDPRTGRASPMPNQNHPPN--RRPEDELGTLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRLSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVRRQSILEDSYRIISSVNRTE--LLKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGLCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFCLDEESFGHFSQRELKPDGANIPLTNENKDEYISLVIQWRFVSRVQDQMNAFLDGFNGLVPLNCVKIFDEHELELLMCGIQNIDVKDWKQNTLYKGDYHPNHITVQWFWRVVLSFNNEMRARLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGLPENYPRAHTCFNRLDLPPYESYQQLRDKLVKAIEGSQGFAGVD 828
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Match: gi|1061128652|ref|XP_017889148.1| (PREDICTED: E3 ubiquitin-protein ligase NEDD4 isoform X3 [Ceratina calcarata]) HSP 1 Score: 1058.13 bits (2735), Expect = 0.000e+0 Identity = 540/850 (63.53%), Postives = 637/850 (74.94%), Query Frame = 0
Query: 1 MAESRIVGYVPPPGS-----TDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPRS----ARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAG-SEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNE---NNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRR-------------TSVPSGSAPSLSSVGGTLPS-PLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHED--DLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
MAE+ GY P PG+ D T LRLKVIAGH LAKKDIFGASDPY+R+DL T+ G++ DS LTKTKK+T+NP WEEEFIFRVKP HKLVL+VFDENRLTRDDFLGMVELTL N+P+E EGR IP ++Y LRPRS RS+VKG L++YHAY+++ +TVE G++A S GWEIV Q V++ V E + D LP GWEERQDANGRTYYVNH+AR TQW+ P + T + E +NA+ +RR HIS DE R RS + V+ E + + R +++ G SG T+S +G +V +R P+GS S + P+ PL+ EGLP W MQ+APNGRVFFI+H+ + TTW+DPRTGRPSS+P+ P D LGPLPEGWEERVHTD RIFFIDHNTRTTQWEDPR+S I GPA+PY+RDYKRKY+YLKSQL+KP+NVPNKFEIKV R ILEDS+R + + ++V++LKTKLW+EF+ E+GLDYGGLAREWF+LLSKEMFNPYYGLFEYSATDNYTLQINP+SG+CNE+H++YFKFIGRIAGMAVYHGKLLD FFIRPFYKMML K I LKDMESVD+EYYNSLLWIKENDP+ELELTF VDEE FG T RELKP+GA + +TNE+K++YI VIQWRFVSRV+EQMN F GFN L+P +L+KIFDE ELELLMCGI IDV+DWK NT+YKGDYH NHIV+QWFWRVVLSF+NEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQL+TIEKWG P ++PRAHTCFNR+DLPPYE
Sbjct: 1 MAENHAYGYNPTPGADGEPREDATSKLRLKVIAGHHLAKKDIFGASDPYVRVDLNTISGDQTIDSALTKTKKKTVNPVWEEEFIFRVKPIEHKLVLQVFDENRLTRDDFLGMVELTLINVPKEQEGRTIPARRYCLRPRSNHSSQRSRVKGTLEVYHAYISD-------SSTVENDNGDTASDSGGWEIV----QPVNSPV---------------EQAADIMMNRPLPPGWEERQDANGRTYYVNHIARFTQWERPTESTTTPSGNMAMERMFDNAATEFQRRYHISADEDR-----HRSSIQDGEVLREENEDSDARDYEIESHRG-GFSGDTSSD--SGRSVDQRGYLSEYEDQSDETVDSPAGSRRSSEQIESPKPTIPLSDEGLPPGWGMQIAPNGRVFFIDHTARTTTWIDPRTGRPSSIPNHIAPATTPRSDLDQLGPLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRMSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVSRNNILEDSYRIISSV--NRVEILKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPFSGVCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLAKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFSVDEESFGHTSQRELKPDGANVPLTNENKDEYIALVIQWRFVSRVQEQMNAFLEGFNALIPPTLVKIFDEHELELLMCGIQHIDVRDWKQNTLYKGDYHANHIVVQWFWRVVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGTPENYPRAHTCFNRIDLPPYE 814 HSP 2 Score: 738.413 bits (1905), Expect = 0.000e+0 Identity = 401/822 (48.78%), Postives = 519/822 (63.14%), Query Frame = 0
Query: 819 EMISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVATTGH---LPDTFYTLRKRSPKSKVRGRL--FLQIENKPDSNPTNTPSSQPQTEPHATGASV-----APLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPE--------------EIELG----SPHTAIPTG-----------------NTRETPSNVVEPCQSSAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
++I+G LAKKDIFGASDPYV V L+ S TI A+T KK++NP W F+FR P H L+ QVFDENR+TRDDFLG+V + L +V +P Y LR RS S R R+ L++ + S+ + + T + G + +P+ + A+ I+ + LP GWEERQ A GRTYYV+H R P+ + GN +E + +T F R R HIS D E +I D E EIE S T+ +G T ++P+ + P+ TIP EGLP GWG+++APN GRVFFI+H R TTW+DPRT RPS +P P + D L LP+GWEE++ DGR FIDH + TQWEDPR++ G + + +YK+KYE +L P +P K I V R++I +DS+ I ++ + + LK +LWVEFEGE GLD+GGLAREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + +HLNYF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F VDE+ G T +L P GA+V +T NK +YI VI WRFVSRVQEQM AF GF IP + +FDE ELELL+CGI ID++DW ++T+Y+G YH NH VVQWFWR VLSFSN+MR+RLLQFVTGTSRVPMNGF L GSN + F I+ +G+P+++PR+HTCFNR+DLP Y SY++LR +L+ A++ ++ F GVD
Sbjct: 30 KVIAGHHLAKKDIFGASDPYVRVDLNTISGDQTID-SALTKTKKKTVNPVWEEEFIFRVKPIEHKLVLQVFDENRLTRDDFLGMVELTLINVPKEQEGRTIPARRYCLRPRSNHSSQRSRVKGTLEVYHAYISDSSTVENDNGDTASDSGGWEIVQPVNSPVEQAAD-IMMNRPLPPGWEERQDANGRTYYVNHIARFTQWERPTEST---TTPSGNMA-MERMFDNAATE-FQR-RYHISAD---EDRHRSSIQDGEVLREENEDSDARDYEIESHRGGFSGDTSSDSGRSVDQRGYLSEYEDQSDETVDSPAGSRRSSEQIESPKPTIPLSDEGLPPGWGMQIAPN-GRVFFIDHTARTTTWIDPRTGRPSSIPNHIAPATTPRSDLDQLGPLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRMSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVSRNNILEDSYRIISSVNRVEI--LKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPFSGVCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLAKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFSVDEESFGHTSQRELKPDGANVPLTNENKDEYIALVIQWRFVSRVQEQMNAFLEGFNALIPPTLVKIFDEHELELLMCGIQHIDVRDWKQNTLYKGDYHANHIVVQWFWRVVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGTPENYPRAHTCFNRIDLPPYESYQQLREKLVKAIEGSQGFAGVD 837
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Match: gi|936700063|ref|XP_014227999.1| (E3 ubiquitin-protein ligase NEDD4 isoform X3 [Trichogramma pretiosum]) HSP 1 Score: 1056.2 bits (2730), Expect = 0.000e+0 Identity = 530/837 (63.32%), Postives = 630/837 (75.27%), Query Frame = 0
Query: 1 MAESRIVGYVPPPGS-----TDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPR--SARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAGSEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNENN---ASELSRRRVHISMDE--QRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPSLSSVGGTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPN----RPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
MAE + GY P + + T LRL+V+AGH LA+KDIFGASDPY+RI+L V+G E D+VLTKTKK+TLNPKWEEEFIFRVKP+ HKLVL+VFDENRLTRDDFLGMVEL+L NLP+E +GR+IP KQY+LRPR S RSKVKG L+LYHAY+++ N + D+ E + + S GWE++ + P+P E S + +A LP GWEERQDANGRTYYVNHVAR TQW+ P T+N + N A+ +RR HIS DE R + + + D + ++ + S++T + P + A T P+ + S +N EGLP W++Q APNGRVFFI+H+E+ TTWVDPRTGRPSS+P +P+ R D +GPLPEGWEER+HTD RIFFIDHNTRTTQWEDPR+ I GPA+PY+RDYKRKY+YLKSQL+KP+NVPNKFEIKV R ILEDS+R + + ++V++LKTKLWIEF+ E+GLDYGGLAREWF+LLSKEMFNPYYGLFEYSA DNYTLQINP+SG+CNE+H++YFKFIGRIAGMAVYHGKLLD FFIRPFYKMML KTI LKDMESVD+EYYNSLLWIKENDP+ELELTF VDE+ FG T RELK NGA I VTNE+K++YI+ VIQWRFVSRV+EQMN F GFN LVPL+L+KIFDE ELELLMCGI IDVKDWK NT+YKGDYHPNHI +QWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQL+TIEKWG P+++PRAHTCFNR+DLPPY+
Sbjct: 1 MAEENVYGYNPSVAADGNDGEEATNRLRLRVVAGHNLARKDIFGASDPYVRIELNNVNGNETVDAVLTKTKKKTLNPKWEEEFIFRVKPNEHKLVLQVFDENRLTRDDFLGMVELSLQNLPKEQDGRSIPVKQYILRPRNNSQRSKVKGTLELYHAYISDSNGA--IDSGDNE---DPSDSGGWELL---------------DQPSPTLETPEEQSAELSALGPLPEGWEERQDANGRTYYVNHVARFTQWERPTATNNVPLGVTQEQRNLDTAATEFQRRYHISADETVDRTAERLANVHVTPDDDAARVENIETEQRVSINTLNNP------VNRADLENQTDDNTDSPTTDSRRPSVQRDDNNRQINGEGLPAGWSIQTAPNGRVFFIDHNERATTWVDPRTGRPSSIPCALAVPSTVQPRNDIDSVGPLPEGWEERLHTDGRIFFIDHNTRTTQWEDPRLQNPNIAGPAVPYSRDYKRKYEYLKSQLRKPTNVPNKFEIKVGRNNILEDSYRIISSV--NRVEILKTKLWIEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGVCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLSKTIDLKDMESVDSEYYNSLLWIKENDPSELELTFCVDEDSFGHTSQRELKTNGANIPVTNENKDEYIQLVIQWRFVSRVQEQMNAFLEGFNALVPLTLVKIFDENELELLMCGIQHIDVKDWKANTLYKGDYHPNHITVQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGTPDNYPRAHTCFNRIDLPPYD 809 HSP 2 Score: 731.48 bits (1887), Expect = 0.000e+0 Identity = 398/827 (48.13%), Postives = 519/827 (62.76%), Query Frame = 0
Query: 811 NRLDLPPYEMISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVATTG---HLPDTFYTLRKR--SPKSKVRGRLFL----------QIENKPDSNPTNTPSSQPQTEPHATGASVAPLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVD------TEAEPGGHPTIDDP----EEIELGSPHTAIPTGNT--------RETPSNVVEPCQSSAPPERTIPQDT-------EGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSE--DFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
NRL L +++G +LA+KDIFGASDPYV + L+ + T+ +T KK+LNP W F+FR P+ H L+ QVFDENR+TRDDFLG+V + L+++ +P Y LR R S +SKV+G L L I++ + +P+++ + +P T + + + P LPEGWEERQ A GRTYYV+H R P+ + + +++ +T F R R HIS D E H T DD E IE +I T N +T N P S P ++ +D EGLP+GW ++ APN GRVFFI+HN R TTWVDPRT RPS +P P + D + LP+GWEE++ DGR FIDH + TQWEDPR+ G + + +YK+KYE +L P +P K I V R++I +DS+ I ++ + + LK +LW+EFEGE GLD+GGLAREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + +HLNYF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F VDED G T +L GA++ VT NK +YI VI WRFVSRVQEQM AF GF +PL + +FDE ELELL+CGI ID++DW +T+Y+G YHPNH VQWFWR VLSF+N+MR+RLLQFVTGTSRVPMNGF L GSN + F I+ +G+PD++PR+HTCFNR+DLP Y SY +LR +L+ A++ ++ F GVD
Sbjct: 25 NRLRL---RVVAGHNLARKDIFGASDPYVRIELNNVNGNETVDA-VLTKTKKKTLNPKWEEEFIFRVKPNEHKLVLQVFDENRLTRDDFLGMVELSLQNLPKEQDGRSIPVKQYILRPRNNSQRSKVKGTLELYHAYISDSNGAIDSGDNEDPSDSGGWELLDQPSPTLETPEEQSAELSALGP---LPEGWEERQDANGRTYYVNHVARFTQWERPTATNNVPLGVTQEQRNLDTAATE-----FQR-RYHISADETVDRTAERLANVHVTPDDDAARVENIET-EQRVSINTLNNPVNRADLENQTDDNTDSPTTDSRRP--SVQRDDNNRQINGEGLPAGWSIQTAPN-GRVFFIDHNERATTWVDPRTGRPSSIPCALAVPSTVQPRNDIDSVGPLPEGWEERLHTDGRIFFIDHNTRTTQWEDPRLQNPNIAGPAVPYSRDYKRKYEYLKSQLRKPTNVPNKFEIKVGRNNILEDSYRIISSVNRVEI--LKTKLWIEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSAMDNYTLQINPFSGVCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLSKTIDLKDMESVDSEYYNSLLWIKENDPSELELTFCVDEDSFGHTSQRELKTNGANIPVTNENKDEYIQLVIQWRFVSRVQEQMNAFLEGFNALVPLTLVKIFDENELELLMCGIQHIDVKDWKANTLYKGDYHPNHITVQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGTPDNYPRAHTCFNRIDLPPYDSYPQLREKLIKAIEGSQGFAGVD 832
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Match: gi|1061128649|ref|XP_017889147.1| (PREDICTED: E3 ubiquitin-protein ligase NEDD4 isoform X2 [Ceratina calcarata]) HSP 1 Score: 1054.66 bits (2726), Expect = 0.000e+0 Identity = 532/825 (64.48%), Postives = 627/825 (76.00%), Query Frame = 0
Query: 17 DRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRTLNPKWEEEFIFRVKPSAHKLVLEVFDENRLTRDDFLGMVELTLANLPRESEGRNIPTKQYLLRPRSARSKVKGNLQLYHAYVAEPNASEEPDTTVEELEGNSAG-SEGWEIVGNSEQTVDTTVTGFEELPAPIRGGGNEGSGDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNE---NNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRR-------------TSVPSGSAPSLSSVGGTLPS-PLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHED--DLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRIST--IGGPAIPYNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAELELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWGNPNSFPRAHTCFNRLDLPPYE 819
D T LRLKVIAGH LAKKDIFGASDPY+R+DL T+ G++ DS LTKTKK+T+NP WEEEFIFRVKP HKLVL+VFDENRLTRDDFLGMVELTL N+P+E EGR IP ++Y LRPRS RS+VKG L++YHAY+++ +TVE G++A S GWEIV Q V++ V E + D LP GWEERQDANGRTYYVNH+AR TQW+ P + T + E +NA+ +RR HIS DE R RS + V+ E + + R +++ G SG T+S + G +V +R P+GS S + P+ PL+ EGLP W MQ+APNGRVFFI+H+ + TTW+DPRTGRPSS+P+ P D LGPLPEGWEERVHTD RIFFIDHNTRTTQWEDPR+S I GPA+PY+RDYKRKY+YLKSQL+KP+NVPNKFEIKV R ILEDS+R + + ++V++LKTKLW+EF+ E+GLDYGGLAREWF+LLSKEMFNPYYGLFEYSATDNYTLQINP+SG+CNE+H++YFKFIGRIAGMAVYHGKLLD FFIRPFYKMML K I LKDMESVD+EYYNSLLWIKENDP+ELELTF VDEE FG T RELKP+GA + +TNE+K++YI VIQWRFVSRV+EQMN F GFN L+P +L+KIFDE ELELLMCGI IDV+DWK NT+YKGDYH NHIV+QWFWRVVLSF+NEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQL+TIEKWG P ++PRAHTCFNR+DLPPYE
Sbjct: 57 DATSKLRLKVIAGHHLAKKDIFGASDPYVRVDLNTISGDQTIDSALTKTKKKTVNPVWEEEFIFRVKPIEHKLVLQVFDENRLTRDDFLGMVELTLINVPKEQEGRTIPARRYCLRPRSQRSRVKGTLEVYHAYISD-------SSTVENDNGDTASDSGGWEIV----QPVNSPV---------------EQAADIMMNRPLPPGWEERQDANGRTYYVNHIARFTQWERPTESTTTPSGNMAMERMFDNAATEFQRRYHISADEDR-----HRSSIQDGEVLREENEDSDARDYEIESHRG-GFSGDTSSDS--GRSVDQRGYLSEYEDQSDETVDSPAGSRRSSEQIESPKPTIPLSDEGLPPGWGMQIAPNGRVFFIDHTARTTTWIDPRTGRPSSIPNHIAPATTPRSDLDQLGPLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRMSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVSRNNILEDSYRIISSV--NRVEILKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPFSGVCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLAKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFSVDEESFGHTSQRELKPDGANVPLTNENKDEYIALVIQWRFVSRVQEQMNAFLEGFNALIPPTLVKIFDEHELELLMCGIQHIDVRDWKQNTLYKGDYHANHIVVQWFWRVVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGTPENYPRAHTCFNRIDLPPYE 845 HSP 2 Score: 742.265 bits (1915), Expect = 0.000e+0 Identity = 401/820 (48.90%), Postives = 521/820 (63.54%), Query Frame = 0
Query: 819 EMISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITRDDFLGLVIVDLKDVATTGH---LPDTFYTLRKRSPKSKVRGRLFLQIENKPDSNPTNTPSSQPQTEPHATGASV-----APLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPE--------------EIELG----SPHTAIPTG-----------------NTRETPSNVVEPCQSSAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIALSATKGSFMTFDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPLELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCIKGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAFDGVD 1595
++I+G LAKKDIFGASDPYV V L+ S TI A+T KK++NP W F+FR P H L+ QVFDENR+TRDDFLG+V + L +V +P Y LR RS +S+V+G L ++ + S+ + + T + G + +P+ + A+ I+ + LP GWEERQ A GRTYYV+H R P+ + GN +E + +T F R R HIS D E +I D E EIE S T+ +G T ++P+ + P+ TIP EGLP GWG+++APN GRVFFI+H R TTW+DPRT RPS +P P + D L LP+GWEE++ DGR FIDH + TQWEDPR++ G + + +YK+KYE +L P +P K I V R++I +DS+ I ++ + + LK +LWVEFEGE GLD+GGLAREWFFLLSKEMFNPYYGLFEYSA D+YTLQINP SG+ + +HLNYF+FIG++AGMAVYHG LLD FFIRPFYKMML + I L DMESVD EYYNSL++IK++DP LEL+F VDE+ G T +L P GA+V +T NK +YI VI WRFVSRVQEQM AF GF IP + +FDE ELELL+CGI ID++DW ++T+Y+G YH NH VVQWFWR VLSFSN+MR+RLLQFVTGTSRVPMNGF L GSN + F I+ +G+P+++PR+HTCFNR+DLP Y SY++LR +L+ A++ ++ F GVD
Sbjct: 65 KVIAGHHLAKKDIFGASDPYVRVDLNTISGDQTID-SALTKTKKKTVNPVWEEEFIFRVKPIEHKLVLQVFDENRLTRDDFLGMVELTLINVPKEQEGRTIPARRYCLRPRSQRSRVKGTL--EVYHAYISDSSTVENDNGDTASDSGGWEIVQPVNSPVEQAAD-IMMNRPLPPGWEERQDANGRTYYVNHIARFTQWERPTEST---TTPSGNMA-MERMFDNAATE-FQR-RYHISAD---EDRHRSSIQDGEVLREENEDSDARDYEIESHRGGFSGDTSSDSGRSVDQRGYLSEYEDQSDETVDSPAGSRRSSEQIESPKPTIPLSDEGLPPGWGMQIAPN-GRVFFIDHTARTTTWIDPRTGRPSSIPNHIAPATTPRSDLDQLGPLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRMSNPQIAGPAVPYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVSRNNILEDSYRIISSVNRVEI--LKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPFSGVCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLAKPIDLKDMESVDSEYYNSLLWIKENDPSELELTFSVDEESFGHTSQRELKPDGANVPLTNENKDEYIALVIQWRFVSRVQEQMNAFLEGFNALIPPTLVKIFDEHELELLMCGIQHIDVRDWKQNTLYKGDYHANHIVVQWFWRVVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIEKWGTPENYPRAHTCFNRIDLPPYESYQQLREKLVKAIEGSQGFAGVD 868 The following BLAST results are available for this feature:
BLAST of e3 ubiquitin-protein ligase nedd-4 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 22
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BLAST of e3 ubiquitin-protein ligase nedd-4 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of e3 ubiquitin-protein ligase nedd-4 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold186_size273091:10951..17763- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold186_size273091-processed-gene-0.4 ID=snap_masked-scaffold186_size273091-processed-gene-0.4|Name=e3 ubiquitin-protein ligase nedd-4|organism=Tigriopus kingsejongensis|type=gene|length=6813bp|location=Sequence derived from alignment at scaffold186_size273091:10951..17763- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'e3 ubiquitin-protein ligase nedd-4' has the following synonyms
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