prolyl 4-hydroxylase subunit alpha-1, snap_masked-scaffold359_size197282-processed-gene-0.4 (gene) Tigriopus kingsejongensis
|
Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Match: EMLSAG00000004330 (supercontig:LSalAtl2s:LSalAtl2s227:607006:610705:1 gene:EMLSAG00000004330 transcript:EMLSAT00000004330 description:"maker-LSalAtl2s227-augustus-gene-6.6") HSP 1 Score: 290.426 bits (742), Expect = 5.426e-86 Identity = 186/546 (34.07%), Postives = 271/546 (49.63%), Query Frame = 0
Query: 1044 KYEFGLFENFVVTEEVFKNEINLIQALKHFHRAAAETP---QNNEGQISQVSQLQHLARLKFELTEKLKPLEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDD-NLEKLKKQPKFVRQMGPYKRNITN--PNEYLQ--------------GDLREEAERRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCG 1569
+Y FG+FEN +++F EI+L+++L++ +E QN E +S +L L+ + P +DGA GLLVL +TY ++ L+G +V + + E L +QD+L L+ KA + WYD+++ +R A P D + + Q N +L G++++V PY++D L+K KKQPK + + Y RN+++ P L DL EE RR+CR E+ + + C LH+ +PYLR+ PFK E++ P RMILH WL K++K N N +I + + D F + +Y + +P+L LS KIE AT++K+ R+S++ YQVTNYGL GLCE HIDPHG L G V R LF +GD+ T M WLDDV+AGG T F PG E ++ P +GSAA W +L G + +SH GCPI+ G+KWILNKW++ FDQFH Y CG
Sbjct: 20 QYAFGVFENSKGLKDIFNFEISLVESLRNTREILSERRNFLQNLENSSHILSP--NLLNLQMSSVNHIDPFH---------YDGASRGLLVLLDTYKFDLRGMLQG-IVRDQSLSIELLGKERLTLQDLLHLSKKAFDYNWYDSSIALLRVXLK-------------AHSDPSTDSYREKLVQIHNIHLKKNLKRVGKNFKVYPYMIDSQTLQKKKKQPKVIGKTTFYNRNVSHIPPPAQLDVQKLKESELAMIEFTDLAEEEFRRICREEI---SPPESSERKCILLHHNDPYLRLGPFKQEIISEDPPRMILH---------WL------------------KDIKFNQEYNLQI----------LRGELNKFDPSFPDWYKEKEPEDIYGFQIVHPILYRLSLKIEHATRLKVTDRWSSSAYQVTNYGLGGLCELHIDPHGMLRGTMVNDDRVSLFKTGDMILTLMGWLDDVEAGGSTAFIYPGREGILKPRKGSAAIWFNLHKNGKKSFSTSHGGCPIIGGTKWILNKWMFYFDQFHGYPCG 500 HSP 2 Score: 278.485 bits (711), Expect = 6.744e-82 Identity = 183/552 (33.15%), Postives = 277/552 (50.18%), Query Frame = 0
Query: 9 TIVLLWHGGTGYDLDSG-YEFHLFENFGLTKDLFLRERMFQSTLTEIKAQLELRLKALEHKSTTLIELESQALTARKNSLNETTAIFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDA-NLERKKKQPKV------------------------LRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPP--PRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDL----FTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
T+VL+ G + S Y F +FEN KD+F E +L + L R L++ + L L + +S+N D YDGA ++VL DTY FD+ QG++ + + + + +L +DL ++ A WYD+SI L+ L P Y R LV+++N ++ K + G N+KV PY++D+ L++KKKQPKV L+E+++ + + + E+ R +C R PP +C LHH DPYLRLGPFK E++S +P ++ L F Q+ +L G+++++ + W Y++K+ E D Y + I +L+ LS K+E ATRL +T ++SS+ YQVTNYGL GLCE H+DPHG L G + R+ L K+GDM T+MGW+ D GG+TAF+YP +E ++ P KG+AA W++L G ++ T HGGCPI+ G+KWI+NKW+FYFDQ
Sbjct: 3 TVVLVLIGHILKETTSNQYAFGVFENSKGLKDIFNFEISLVESLRNTREILSERRNFLQNLENSSHILSPNLLNLQMSSVNHI-----DPFHYDGASRGLLVLLDTYKFDLRGMLQGIVRDQSLS----IELLGKERLTLQDLLHLSKKAFDYNWYDSSIALLR------VXLKAHSDPSTDSY---REKLVQIHNIHLKKNLKRVGKNFKVYPYMIDSQTLQKKKKQPKVIGKTTFYNRNVSHIPPPAQLDVQKLKESELAMIEFTDLAEEEF--RRIC-----REEISPPESSERKCILLHHNDPYLRLGPFKQEIISEDPPRMILHWLKDIKFNQEYNLQIL-----------------RGELNKFDP---------SFPDW-----YKEKEPE-----DIYGFQIVHPILYRLSLKIEHATRLKVTDRWSSSAYQVTNYGLGGLCELHIDPHGMLRGTMVNDDRVSLFKTGDMILTLMGWLDDVEAGGSTAFIYPGREGILKPRKGSAAIWFNLHKNGKKSFSTSHGGCPIIGGTKWILNKWMFYFDQ 493 HSP 3 Score: 249.98 bits (637), Expect = 5.405e-72 Identity = 170/550 (30.91%), Postives = 256/550 (46.55%), Query Frame = 0
Query: 536 SEIQTVKSMRELQNNLAQLSQALEEKELVTPPVKEAITVKKVQISNLMATFPEEQTIVDTAKGLLMVMDTYRIQIDALTK---RDWDFLHPLRGRVRLVNHQPMGADDTLTLAFEAFKIGWYDHAANLTRFTREHLSLASFSPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDG-NLKKKSSQPKFLKREDFFNRD-----PPEVLNQEYEKK------------EDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFN---------ATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWIAWLGLAMGGKYEFGLFENFVV 1055
S ++++++ RE+ + Q LE + P + +Q+S++ P ++GLL+++DTY+ + + + RD L G+ RL D L L+ +AF WYD + L R H + + + LV+ HN L++ VG+ FK+ PY++D L+KK QPK + + F+NR+ PP L+ + K+ E+ RRICR EI E + +C LHHN+P+LRL PFK E++ + P RMILH W++D+ FN L+ S W E D Y +I P +++LS KIE AT + +T+RWSS+ YQVTNYGLGGLCE HIDPHG+ G + L TGDM+ T M WL+DV+AGG TAF G E + P +GSAA W NL + ++ +SHGGCP++ G+K +++ W+ + G FE++ V
Sbjct: 41 SLVESLRNTREILSERRNFLQNLENSSHILSP-----NLLNLQMSSVNHIDPFHYD--GASRGLLVLLDTYKFDLRGMLQGIVRDQSLSIELLGKERLT------LQDLLHLSKKAFDYNWYDSSIALLR--------VXLKAHSDPSTDSYREKLVQIHNIHLKKNLKRVGKNFKVYPYMIDSQTLQKKKKQPKVIGKTTFYNRNVSHIPPPAQLDVQKLKESELAMIEFTDLAEEEFRRICREEISPPESSER----KCILLHHNDPYLRLGPFKQEIISEDPPRMILH----------------------------------------------------WLKDIKFNQEYNLQILRGELNKFDPSFPDWYKEKEPEDIYGFQIVHPILYRLSLKIEHATRLKVTDRWSSSAYQVTNYGLGGLCELHIDPHGMLRGTMVNDDRVSLFKTGDMILTLMGWLDDVEAGGSTAFIYPGREGILKPRKGSAAIWFNLHKNGKKSFSTSHGGCPIIGGTKW----ILNKWMFYFDQFHGYPCGLNEFEDYKV 509
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Match: EMLSAG00000012413 (supercontig:LSalAtl2s:LSalAtl2s909:41472:46417:1 gene:EMLSAG00000012413 transcript:EMLSAT00000012413 description:"augustus_masked-LSalAtl2s909-processed-gene-0.4") HSP 1 Score: 167.162 bits (422), Expect = 1.260e-43 Identity = 137/499 (27.45%), Postives = 233/499 (46.69%), Query Frame = 0
Query: 1097 LARLKFE---LTEKLKPLEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQ----DILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYR--VLPY--------------LVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAER--RVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVS--LSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKC 1568
L +L FE L E+L K E + GA+ G+ +L+ETY+L DF +NG+I ++ +P +++Q D+ +L + + +YD AV ++ +AL + KD + +++ + ++ + +L R +P G D+R V P+ ++ + K P R++ P RE+ E+ R+CRGE + + DE ++LH+ +PYL++ PFK+EV P +++ DF+ + E Y++ +LSRS +N N+++ + + QTW+ F+++ + +S ++++IE AT + + + YQV NYGL G+ H+D G + +P GD AT MA+L DV AGGGT F S G + V P +G+A FW ++ G ++ H GCP+L GSKWI NKWI + + C
Sbjct: 103 LTKLIFENERLLEELSQFPK-----EKEMYGAMNGIFLLQETYDLDPLDF------SNGIIQNKYMTPITVDIQLKIDDMEYLGKLSYNRGYYDRAVDWIM----AALMKNNKNKDSSNYIR--LNKTLSTLMRAHDEFLEKRGSPAGSDWRTYVTPFNERLAKKKKYKRALKKNNGNRRYKMVPILGRRLNP----------------REKWEQFNRLCRGENL-RSPSDEVQLKSFYLHHNDPYLKLGPFKVEVNNIEPSLIVIKDFIFDREGESYRNYAEDKLSRS---------FSLSNDGNRQVTLTRTSKQTWL----------FEKN-----------------LTISKIITKRIELATGLVAETLYGNEPYQVANYGLGGVYNSHMDSGGTI----MP----EFNELGDRVATVMAYLSDVGAGGGTTFPSLGXK--VTPEKGAAVFWYNIDKNGMQDYNMFHGGCPVLTGSKWITNKWIRQLSNWEKHSC 521 HSP 2 Score: 160.999 bits (406), Expect = 1.293e-41 Identity = 132/488 (27.05%), Postives = 230/488 (47.13%), Query Frame = 0
Query: 54 IKAQLELRLKALEHKSTTLIELESQALTARKNSLNETTAIFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYESMRRSL---VKVNNEYITKKMIMAGANYK--VLPY-------------VVDANLERKKKQPKVLRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
I A + L+ AL+ T++ L L E + FP + GA+ + +L++TYD D + G+I + P+ ++ K+D D++ + ++ + +YD ++ ++ + A + + S Y + ++L ++ ++E++ K+ AG++++ V P+ + N R+ K +L + + E + R +C G + RS + + + YLHH DPYL+LGPFKVE+ + P L+V +D +E + + L+R+ L N+G ++ + + Q WL + N TI SK++ ++++E AT L Y + YQV NYGL G+ +H+D G++ +P + GD AT+M ++ D GG T F P V P KGAA +WY++D G ++ HGGCP+L GSKWI NKWI
Sbjct: 84 ISATMVLKRCALDWIKITVL----TKLIFENERLLEELSQFPKEKEMYGAMNGIFLLQETYDLDPLDFSNGIIQNKYMT---PITVDIQLKID--DMEYLGKLSYNRGYYDRAVDWI-----MAALMKNNKNKDSSNYIRLNKTLSTLMRAHDEFLEKRGSPAGSDWRTYVTPFNERLAKKKKYKRALKKNNGNRRYKMVPILGRR------LNPREKWEQFNR-LCRGENLRSPSDEV-QLKSFYLHHNDPYLKLGPFKVEVNNIEPSLIVIKDFIFDREGESYRNYAEDKLSRSF--SLSNDGN-------RQVTLTRTSKQTWLFE----------------KNLTI-SKII---TKRIELATGLVAETLYGNEPYQVANYGLGGVYNSHMDSGGTI----MPE----FNELGDRVATVMAYLSDVGAGGGTTF--PSLGXKVTPEKGAAVFWYNIDKNGMQDYNMFHGGCPVLTGSKWITNKWI 510 HSP 3 Score: 135.191 bits (339), Expect = 4.121e-33 Identity = 136/541 (25.14%), Postives = 245/541 (45.29%), Query Frame = 0
Query: 523 IFYFDQSTEALIRSEIQTVKSMRELQNNLAQLSQALEEK-ELVTP----------PVKEAITVKK-----VQISNL-------------MATFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDW----DFLHPLRGRVRLVNHQPMGADDTLTLAFEAFKIGWYDHAAN--LTRFTREHLSLASFSPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFK--LLPYLVDGNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEKKE--DVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTV-QCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
+F ++ E+L + EIQ K + NNL ++ +L+ + +TP P+ + +K+ ++I+ L ++ FP+E+ + G+ ++ +TY +D L + ++ P+ ++L DD L ++ G+YD A + + + + + S I L + L+R H+E L ++ + G ++ + P+ KKK + + + P +L + +E + R+CRGE + + ++V+ + +LHHN+P+L+L PFK+EV P +++ + + + E + +Y+ LSR +S G R+ + +T Q W+ F+ + S+I ++K+IE AT ++ + + YQV NYGLGG+ SH+D G I P + GD +AT MA+L+DV AGG T F S G + V P +G+A FW N+ + ++ HGGCPVL GSK
Sbjct: 28 LFSSARAIESLYKKEIQINKDIELYINNLEEVLLSLKSTMKSITPDVRKEIHPNNPISATMVLKRCALDWIKITVLTKLIFENERLLEELSQFPKEKEMYGAMNGIFLLQETY--DLDPLDFSNGIIQNKYMTPITVDIQL------KIDDMEYLGKLSYNRGYYDRAVDWIMAALMKNNKNKDS---SNYIRLNKTLSTLMRAHDEFLEKRGSPAGSDWRTYVTPFNERLAKKKKYKRALKKNNGNRRYKMVP-ILGRRLNPREKWEQFNRLCRGENLRSP-SDEVQL-KSFYLHHNDPYLKLGPFKVEVNNIEPSLIVIKDFIFDREGESYRNYAEDKLSRSFSLSND-----------GNRQVTLTRTSKQTWL-----------------------FEKNLTISKI-------ITKRIELATGLVAETLYGNEPYQVANYGLGGVYNSHMD-----SGGTIMPE---FNELGDRVATVMAYLSDVGAGGGTTFPSLGXK--VTPEKGAAVFWYNIDKNGMQDYNMFHGGCPVLTGSK 503
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Match: EMLSAG00000004404 (supercontig:LSalAtl2s:LSalAtl2s231:1078605:1080545:-1 gene:EMLSAG00000004404 transcript:EMLSAT00000004404 description:"snap_masked-LSalAtl2s231-processed-gene-10.3") HSP 1 Score: 160.999 bits (406), Expect = 2.905e-41 Identity = 138/464 (29.74%), Postives = 220/464 (47.41%), Query Frame = 0
Query: 1121 DFDGAITGLLVLKETYNLPMKDFLR-GKVVTN-GLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRV--LPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAE-RRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYH-----GDDEFDQDDQLVPNPKL-YNYTD----TNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFAS-GDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKC 1568
D+ A +GL +L E Y L + +F + GK+ + LIP P + DI + + + W D + M+ +L +K+++ ++ ++D K +I + L R P G+ +R +P+ + +K K+ + Y+ I + E L+ ++ A + CR + +KV E + CY +HN NP+ ++ PFKME+L PF +I DFMS+ E + + RL RS+ + + + +K +I + Q W+ ++ ++E + P NY D N + ++++I RATK+ + +SA YQ+ NYGL G + H D G+ E V R + + GD ATFM +L DV GG T F P RG A FWI+L G + R+ H GCP+L GSKWI NKWI Q H Y C
Sbjct: 148 DYYEACSGLSLLIEAYQLNLTEFCKLGKIGEHMSLIP-----PGWSILTDI---GISSINRGWXDTGITMMQH----SLQAYSGKKEEYQIYKKELD--KSIIMHDK---LLQLRGPIGKRHRTFRVPFSKELRKKKKYKKILKTGEDIRYRPTIEDNYESLK--YKDSALFHKHCRKK---EKVL-ESHERCYLIHNDNPFYKLLPFKMELLSESPFIVIFRDFMSDVETKHFTDIAGRRLRRSQ-----TGSLTEVEGKEEKGELIRTSKQIWLXESTFNISQFLTEEEIQMITKGTGRPHFPGNYKDHLVILNEIGFRITKRIGRATKLNVEDAWSAEAYQIANYGLGGQYDVHFDARGHFEYPPVDEITRFGYENYGDRLATFMVYLRDVPMGGATVF--PKINIYSNAQRGDAVFWINLLSDGTLDTRTLHGGCPVLVGSKWITNKWIASNVQMHLYPC 581 HSP 2 Score: 145.976 bits (367), Expect = 2.460e-36 Identity = 85/265 (32.08%), Postives = 139/265 (52.45%), Query Frame = 0
Query: 278 RCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKV----EVKDIDD---------NY--NYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPP----SRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
RC +H+ +P+ +L PFK+E++S +P++V+F+D + E H + L R++ L +K ++ + Q WL + + + E++ I NY + I +++ F +++++ RAT+LN+ +S+ YQ+ NYGL G + H D G E PP +R G GD AT M +++D P+GGAT V+P + +G A +W +L G + RT+HGGCP+L GSKWI NKWI
Sbjct: 316 RCYLIHNDNPFYKLLPFKMELLSESPFIVIFRDFMSDVETKHFTDIAGRRLRRSQTGSLTEVEG-----KEEKGELIRTSKQIWLXESTFNISQFLTEEEIQMITKGTGRPHFPGNYKDHLVILNEIGFRITKRIGRATKLNVEDAWSAEAYQIANYGLGGQYDVHFDARGHFE---YPPVDEITRFGYENYGDRLATFMVYLRDVPMGGAT--VFPKINIYSNAQRGDAVFWINLLSDGTLDTRTLHGGCPVLVGSKWITNKWI 570 HSP 3 Score: 139.043 bits (349), Expect = 4.650e-34 Identity = 88/271 (32.47%), Postives = 139/271 (51.29%), Query Frame = 0
Query: 773 QRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASR-------IFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHST-GDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWIA 1035
+RC+ +H++NPF +L PFKME+L +SPF +I + +++ E + D + L R + S + ++ + ++ +I + Q W+ + FN + E I + G P+ I + +++K+I +AT + + + WS+ YQ+ NYGLGG + H D G +E + +RF + GD LATFM +L DV GG T F RG A FWINL +D + + HGGCPVL GSK + + WIA
Sbjct: 315 ERCYLIHNDNPFYKLLPFKMELLSESPFIVIFRDFMSDVETKHFTDIAGRRLRRSQTGSLTEVEGKE------EKGELIRTSKQIWLXESTFN--ISQFLTEEEIQMITKGTGRPHFPGNYKDHLVILNEIGFRITKRIGRATKLNVEDAWSAEAYQIANYGLGGQYDVHFDARGHFEYPPVDEITRFGYENYGDRLATFMVYLRDVPMGGATVFPKINIYSNA--QRGDAVFWINLLSDGTLDTRTLHGGCPVLVGSKW----ITNKWIA 571
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Match: EMLSAG00000005013 (supercontig:LSalAtl2s:LSalAtl2s260:584077:587279:-1 gene:EMLSAG00000005013 transcript:EMLSAT00000005013 description:"maker-LSalAtl2s260-snap-gene-6.18") HSP 1 Score: 107.842 bits (268), Expect = 1.839e-24 Identity = 94/353 (26.63%), Postives = 158/353 (44.76%), Query Frame = 0
Query: 1121 DFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDR-LKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEY-LQGDLRE----EAERRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHG 1467
D +GA GL +L+ETYNL + F + ++ LN D+ + A K+ YD A ++ A+ E + + QI + +K VIK + L TR +P G +R V+ KL + K+ + +++ EY L +L++ + +CRGE + + D+ SC +LH +PYL++ PFK+E L PF + HDF+ + E+ +S+P L RS+ + N + ++ + QTW+ D + Q N N + +S +I ATK+ + S+ DYQ+ NYG+ G+ H+D G
Sbjct: 126 DIEGAANGLFLLQETYNLDIDLFAK-------------ENNAALNGNDMSLMGKLAFNKQVYDRAYEWLISSLKKAI-----EDNDSVETISQIKKDIKTVIKY-HDQILETRGSPRGSTWRTY---VNPLEPKLASKNKYKNALKANNGSMSYKMEYKLLTELKDYEITDQFNALCRGEDIRDPI-DDKDLSCSYLHFNDPYLKLGPFKLENLNSYPFIGVYHDFLYDNEIESFKSHSRPLLERSQHSGKKN---------SNEVSFKRTSKQTWL----------VDAETQ--------NNNTVNTLANKISNRISYATKLAVKSKKGGEDYQIANYGMGGVYTPHMDSFG 428 HSP 2 Score: 100.138 bits (248), Expect = 6.086e-22 Identity = 83/361 (22.99%), Postives = 165/361 (45.71%), Query Frame = 0
Query: 87 LNETTAIFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEA-----QRPVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMG----VQSAREYYL----RAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGS 434
++E + P+ D +GA + +L++TY+ +DI+ FA E+ L+ D+ M +A K YD + ++L I++L +A +S+ + ++++K +++ + + G+ ++ ++ L K K L+ N +++ + ++Y + A+C G R + C+YLH DPYL+LGPFK+E +++ P++ V+ D E++ S P L R++ KN ++ ++ K Q WL V+ + ++N T+ +K+ S ++ AT+L + K YQ+ NYG+ G+ H+D G+
Sbjct: 114 ISELLSGLPNSGDIEGAANGLFLLQETYN----------LDIDLFAK------ENNAALNGNDMSLMGKLAFNKQVYDRAYEWL------ISSLKKAIEDNDSVETISQIKKDIKTVIKYHDQILETRGSPRGSTWRTYVNPLEPKLASKNKYKNALKANNGSMSYKMEYKLLTELKDYEITDQFNALCRGEDIRDPIDDKD-LSCSYLHFNDPYLKLGPFKLENLNSYPFIGVYHDFLYDNEIESFKSHSRPLLERSQHSGKKNSNEVSFKRTSK---------QTWL---------VDAETQNNNTVNTLANKI----SNRISYATKLAVKSKKGGEDYQIANYGMGGVYTPHMDSFGA 429 HSP 3 Score: 93.2041 bits (230), Expect = 1.014e-19 Identity = 91/401 (22.69%), Postives = 167/401 (41.65%), Query Frame = 0
Query: 577 VQISNLMATFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQPMGADDTLTLAFEAFKIGWYDHA-----ANLTRFTREHLSLASFSPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDGNLKKKSSQPKFLKREDFFNRDPPEV-LNQEYEKKE--DVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDV--MFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAW 967
+IS L++ P I A GL ++ +TY + ID K N+ + +D + AF YD A ++L + ++ S+ + S +++ K +++ H+++L + + G ++ ++ L K+ LK + E L E + E D +CRGE + + + + C +LH N+P+L+L PFK+E L PF + H+ L ++EI+ +S P L R + N + V+ KR + Q W+ D N T++ + + K+S +I AT + + + YQ+ NYG+GG+ H+D G + GS+++ T + T+ W
Sbjct: 112 TEISELLSGLPNSGDIEGAANGLFLLQETYNLDIDLFAKE---------------NNAALNGNDMSLMGKLAFNKQVYDRAYEWLISSLKKAIEDNDSVETIS-----QIKKDIKTVIKYHDQILETRGSPRGSTWRTYVNPLEPKLASKNKYKNALKANNGSMSYKMEYKLLTELKDYEITDQFNALCRGEDIRDPIDD--KDLSCSYLHFNDPYLKLGPFKLENLNSYPFIGVYHDFLYDNEIESFKSHSRPLLERSQHSGKKN-----SNEVSFKR-----TSKQTWLVDAETQNNNTVNTLAN-------------------------KISNRISYATKLAVKSKKGGEDYQIANYGMGGVYTPHMDSFGA-NRCPVISGSKWI--TNKWVKTYWQW 452
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Match: EMLSAG00000012326 (supercontig:LSalAtl2s:LSalAtl2s8:1477583:1479313:1 gene:EMLSAG00000012326 transcript:EMLSAT00000012326 description:"augustus_masked-LSalAtl2s8-processed-gene-15.1") HSP 1 Score: 105.531 bits (262), Expect = 1.136e-23 Identity = 121/445 (27.19%), Postives = 181/445 (40.67%), Query Frame = 0
Query: 91 TAIFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSAR--EYYLRAVCNGFSFRSYFEPP--PRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNI-THKYSSTQYQVTNYGLAGLCETHVDPHGS--LEGVELPPSRLGLRKS-----GDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
+ I P D G+ A++ + D YD DV ++G+I+ E+ KL KD M + S+++ + + + P +S+ E +R L EY + Q LR +Q+ M V S R E +++C G R EP +C PY L P VE V+ +P++VV+ D+ + EM + + P+L R+ + Q Y K R +KT WL D + I SK+ F K+ T LN T K + Q+ NY G H PH ++GV+ P + K GD AT M ++ + GG T V+P + V P GAA +WY+L G + RT+HG CP+L GSKW+ NKWI
Sbjct: 100 STIIPKEQDLHGSAQALLRISDVYDLDVRNLSEGIIE----------NRETSAKLSAKDCLFMGKHSFNSGELSRSLEWFEQAWLLAGXKPNLXATTLSRDE-VRNFL-----EYAS--------------------------QEHDLRRDQLR---MNVPSPRSEEDMFKSLCRGEEHR---EPQVLANMKCYTTTKTHPYYLLHPVMVEEVNHDPHIVVYYDVISSNEMKVIKELAEPYLMRS-------QVQSETYSEVSKTR-TSKT--GWLQDSFH----------------PIVSKMSF----KVNSMTGLNSDTWKEDAELLQIANYINGG----HYQPHHDYVIKGVQ-PDKMIYGNKGIDYYIGDRIATWMVYMNNVISGGRT--VFPRLGLGVEPIPGAAVFWYNLKKSGEADDRTLHGACPVLLGSKWVSNKWI 459 HSP 2 Score: 93.5893 bits (231), Expect = 7.878e-20 Identity = 82/304 (26.97%), Postives = 137/304 (45.07%), Query Frame = 0
Query: 1284 EEAERRVCRGELVGQKVKDEGPQ-----SCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQT-WIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLS-RFSATDYQVTNYGLSGLCEGHIDPHG--YLEGME----VPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPND 1574
E+ + +CRGE + PQ CY +PY + P +E + P ++ +D +S EM + E ++P L RS+ + E+ EV K ++T++T W+ D F +P++ +S K+ T + + + A Q+ NY ++G GH PH ++G++ + ++ + GD AT+M ++++V +GG T F G V P G+A FW +L+ G + R+ H CP+L GSKW+ NKWI Q CG D
Sbjct: 221 EDMFKSLCRGE------EHREPQVLANMKCYTTTKTHPYYLLHPVMVEEVNHDPHIVVYYDVISSNEMKVIKELAEPYLMRSQVQSETYSEVSK-----------TRTSKTGWL-------QDSF------------------HPIVSKMSFKVNSMTGLNSDTWKEDAELLQIANY-ING---GHYQPHHDYVIKGVQPDKMIYGNKGIDYYIGDRIATWMVYMNNVISGGRTVFPRLGLG--VEPIPGAAVFWYNLKKSGEADDRTLHGACPVLLGSKWVSNKWIREGGQMFTRPCGLESTD 476 HSP 3 Score: 77.411 bits (189), Expect = 1.066e-14 Identity = 100/453 (22.08%), Postives = 176/453 (38.85%), Query Frame = 0
Query: 576 KVQISNLMATFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQPMGADDTLTLAFEAFKIGWYDHAANLTRFTREHLSLASFSPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDGNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEKKEDVMRRICRGEIVGAEVKNQVRFQ-RCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTK--YQVTNYGLGGLCESHID--PHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
K+ L P+EQ + +A+ LL + D Y + + L++ + +L A D L + +F G + + +L F + L LA P+ + ++D VR N + +SQ L+R+ P +ED+ + +CRGE + QV +C+ +P+ L P +E + P ++ +++++ +E++ + + + P L R S ++ + V+ R + W++D F P + K+S K+ T + ++ W Q+ NY GG + H D G+ I + GD +AT+M ++N+V +GG T F G V P G+A FW NL + + HG CPVL GSK
Sbjct: 93 KIHKKKLSTIIPKEQDLHGSAQALLRISDVYDLDVRNLSE---GIIENRETSAKL------SAKDCLFMGKHSFNSG--ELSRSLEWFEQAWL-LAGXKPN--LXATTLSRDEVR--------------------------NFLEYASQEHDLRRDQLRMNVPSP------RSEEDMFKSLCRGE---EHREPQVLANMKCYTTTKTHPYYLLHPVMVEEVNHDPHIVVYYDVISSNEMKVIKELAEPYLMR------SQVQSETYSEVSKTR-----TSKTGWLQDS------------------------------FHPIVSKMSFKVNSMTGLN-SDTWKEDAELLQIANYINGGHYQPHHDYVIKGVQPDKMIYGNKGIDYYIGDRIATWMVYMNNVISGGRTVFPRLGL--GVEPIPGAAVFWYNLKKSGEADDRTLHGACPVLLGSK 452
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Match: EMLSAG00000004188 (supercontig:LSalAtl2s:LSalAtl2s221:321106:325044:-1 gene:EMLSAG00000004188 transcript:EMLSAT00000004188 description:"maker-LSalAtl2s221-augustus-gene-3.12") HSP 1 Score: 104.76 bits (260), Expect = 7.090e-23 Identity = 82/274 (29.93%), Postives = 124/274 (45.26%), Query Frame = 0
Query: 261 VCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLT----RTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYS-STQYQVTNYGLAGLCETHVDPHG-SLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
+C+G FR+ E RC Y +P+L+LGP K E+++ +PY+ F++L +E D++ +P L R + LKN+ + W VE DDN KVL L+ ++E L K + S ++ NYG+ G TH D S E ++P S++ + +T+M +Q GGAT V+P+ +P KG W +L G+ + T H CP+L G KWI NKWI Y Q
Sbjct: 458 LCSGVKFRTPKEDA-DLRCFYADSGNPWLKLGPIKTEILNYDPYVAAFRELLFDEECDNITEYLSPKLDFPPGRMKSNSLKNDWTMK---------------NTW---------PVE----DDN-------KVLEKLTLRVEAVMDLLANSKNNESDKFMCGNYGIGGHYGTHPDFSAYSHEEFKVPGSKV------NRISTVMTILQSPSAGGAT--VFPYTGAYSYPEKGTGIGWXNLKDDGYPDELTRHAACPVLFGQKWIGNKWIGYTPQ 687 HSP 2 Score: 88.1965 bits (217), Expect = 8.523e-18 Identity = 102/474 (21.52%), Postives = 181/474 (38.19%), Query Frame = 0
Query: 1121 DFDGAITGLLVLKETYNLPMKDFLR-GKVVTN-GLIPQEFQSPYH--LNVQDILFLAVKANEKRWYDAAV-----------------PFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRA---PFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATD-YQVTNYGLSGLCEGHIDPHGYL-EGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKC 1568
D GA G+ L Y L +R G+VV G + P H LN D+ +A +A R ++A P + VFN +T + E D ++ + V + + + ++ R P G + + ++ + Y+ + + Y++ ++ +C G + + K++ C++ + NP+L++ P K E+L P+ + + + E + EY P+L +++SN ++N TW D N +L L+ ++E + S+ + +D + NYG+ G H D Y E +VP S+ + +T M L AGG T F G +P +G+ W +L+ G+ + + H CP+L G KWI NKWI Q + C
Sbjct: 270 DIQGASFGIARLISLYELNATSLVREGRVVGELGDWQTIYSEPSHVSLNAWDVYSIAAEAFSNRLFNAGYDMLDLALKKLMEEKINPPNDQVVFN--VTATHLEMDVVRSSLKKVIEMHNVFLKKRGNISSSHRTHDHPVGLKKPKKKSKKKISKNRFAIMDNLIKDLETYENALKSEETYVKTPIQTNL---LCSG-VKFRTPKEDADLRCFYADSGNPWLKLGPIKTEILNYDPYVAAFRELLFDEECDNITEYLSPKLDFPPGRMKSNSLKNDWTMKN-----------TW-------------------------PVEDDNKVLEKLTLRVEAVMDLLANSKNNESDKFMCGNYGIGGHYGTHPDFSAYSHEEFKVPGSKV------NRISTVMTILQSPSAGGATVFPYTG--AYSYPEKGTGIGWXNLKDDGYPDELTRHAACPVLFGQKWIGNKWIGYTPQIYKKPC 693 HSP 3 Score: 73.1738 bits (178), Expect = 3.654e-13 Identity = 65/268 (24.25%), Postives = 103/268 (38.43%), Query Frame = 0
Query: 774 RCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWS-STKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWIA--WLG 1038
RC + NP+L+L P K E+L P+ E+L ++E + +Y +P L G SN ++K+ + P + D + + KL+ ++E +++ + + S K+ NYG+GG +H D PGS+ + ++T M L AGG T F G P +G+ W NL D + L+ H CPVL G K WI W+G
Sbjct: 474 RCFYADSGNPWLKLGPIKTEILNYDPYVAAFRELLFDEECDNITEYLSPKLDFPPGRMKSN-----------------------------------SLKNDWTMKNTWPVEDD-------NKVLEKLTLRVEAVMDLLANSKNNESDKFMCGNYGIGGHYGTHPDFSAYSHEEFKVPGSKV-----NRISTVMTILQSPSAGGATVFPYTGAYS--YPEKGTGIGWXNLKDDGYPDELTRHAACPVLFGQK---------WIGNKWIG 683
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Match: EMLSAG00000011700 (supercontig:LSalAtl2s:LSalAtl2s812:79332:86643:-1 gene:EMLSAG00000011700 transcript:EMLSAT00000011700 description:"maker-LSalAtl2s812-augustus-gene-0.6") HSP 1 Score: 104.76 bits (260), Expect = 1.369e-22 Identity = 111/435 (25.52%), Postives = 180/435 (41.38%), Query Frame = 0
Query: 94 FPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVV-FQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKS----GDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
PD D A A+ + YD I+ +F+NG L ++ L +D + A + ++ +L P + + + ++ +L +VN + K M + PY++ A + K + P ++ N R + A+C G S FE + C Y + L+L P KVE+ P+L++ F DLFTQ E H LIES + +L + G + K+ RI + W+ D + I D + IE+ + +L+ + K QV NYGL G + H D + + P + +S GD +T M ++ D GG TAF P V P KG+A +W+++ G + +HG CP++ GSKW+ NKWI
Sbjct: 146 LPDGKDLQTAAKALNRIHKIYD----------INTTEFSNGNILGHQTSANLGLRDTLYLGRFAANTDYPGVALNWLNTAQSQANLHPNSSEEALKQ---VKLALRQVNRKLSKKPKSMGDKD----PYILGA-IPDKTEDPNLVISNT---------DRRNF--EALCRGEKLISSFEES-KLYCYYDNRGVFGLKLSPLKVEINHREPHLILTFHDLFTQSE-THDLIESV-------RSDLTSAG-VGTDKTISDMRISSHV---WIPD--------GMLSIVDKISEKIENVIGLRSMAQLDPEAK-----KEEYEMLQVANYGLGGHYDCHHDSMFIYKEPQFIPKSVEETQSPYITGDRMSTFMIYLSDVQKGGNTAF--PRLGVATSPRKGSAVFWHNIKRSGRSDMHMIHGACPVVMGSKWVANKWI 523 HSP 2 Score: 97.0561 bits (240), Expect = 3.431e-20 Identity = 87/325 (26.77%), Postives = 140/325 (43.08%), Query Frame = 0
Query: 1253 KLKKQPKFVRQMGPY-----KRNITNPNEYLQGDLREEAERRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMI-LHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATD-------YQVTNYGLSGLCEGHIDP-HGYLEGMEVPASRRRL---FASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLF 1560
KL K+PK + PY +PN + R E +CRGE + +E CY+ + L+++P K+E+ R P ++ HD +++E L+E + L+ + V ++K + +RI ++ WI P+ L ++ +S KIE ++ +++ QV NYGL G + H D Y E +P S + +GD +TFM +L DV GG T F P P +GSA FW +++ G ++ H CP++ GSKW+ NKWI F
Sbjct: 243 KLSKKPKSMGDKDPYILGAIPDKTEDPNLVISNTDRRNFEA-LCRGEKLISSF-EESKLYCYYDNRGVFGLKLSPLKVEINHREPHLILTFHDLFTQSETHDLIESVRSDLTSA--GVGTDKTISD-------MRI---SSHVWI------------------PDGML-------SIVDKISEKIENVIGLRSMAQLDPEAKKEEYEMLQVANYGLGGHYDCHHDSMFIYKEPQFIPKSVEETQSPYITGDRMSTFMIYLSDVQKGGNTAF--PRLGVATSPRKGSAVFWHNIKRSGRSDMHMIHGACPVVMGSKWVANKWIREF 526 HSP 3 Score: 85.5001 bits (210), Expect = 1.161e-16 Identity = 85/327 (25.99%), Postives = 142/327 (43.43%), Query Frame = 0
Query: 719 KKKSSQPKFLKREDFFNRDPPEVLNQEYEKKED-------VMRR----ICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMI-LHEILNEDEIQWLM-----DYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPF-QGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGS----RFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
+K S +PK + +D P +L +K ED RR +CRGE + + + + C++ + L+L+P K+E+ + P ++ H++ + E L+ D ++ + ++ IS +++++ H WI D M + + E + L Q DP A + + M+ QV NYGLGG + H D I++ + P S + + TGD ++TFM +L+DV+ GG TAF G + P +GSA FW N+ R + HG CPV+ GSK
Sbjct: 242 RKLSKKPKSMGDKD------PYILGAIPDKTEDPNLVISNTDRRNFEALCRGEKLISSFEESKLY--CYYDNRGVFGLKLSPLKVEINHREPHLILTFHDLFTQSETHDLIESVRSDLTSAGVGTDKTIS--DMRISSH----------------VWIPDGMLSIVDKISEKIENVIGLRSMAQLDPEAKK--------------EEYEML----------QVANYGLGGHYDCHHDSMFIYKEPQFIPKSVEETQSPYITGDRMSTFMIYLSDVQKGGNTAFPRLGVATS--PRKGSAVFWHNIKRSGRSDMHMIHGACPVVMGSK 516
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Match: EMLSAG00000009563 (supercontig:LSalAtl2s:LSalAtl2s615:626046:628492:-1 gene:EMLSAG00000009563 transcript:EMLSAT00000009563 description:"maker-LSalAtl2s615-augustus-gene-5.47") HSP 1 Score: 102.449 bits (254), Expect = 1.925e-22 Identity = 76/285 (26.67%), Postives = 129/285 (45.26%), Query Frame = 0
Query: 1288 RRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYP 1572
+R+CRGE +K K + C+ ++P+++IAP K E L +P I H+ +S+ E+ + + PR R+ + E++ N + ISKT WI S + N ++ + +++ T + + + + + QV+NYG+ G H DPH E + L +G+ AT++ + +V+ GG T F P + +WP +GSA FW +L G + H P+LKG+K + N WI+ Q C P
Sbjct: 294 QRLCRGE-SNEKKKFDRDLKCFLDFGKHPFMKIAPIKAEKLYSKPDIYIFHNVISDNEIETIKNMANPRFKRATVQNAKTGELETANYR------ISKTA--WIKS-------------------------EENDVVKGIYQRVHDVTGLNMET---SEELQVSNYGIGG----HYDPHFDFARREEKDAFSSL-GTGNRIATWLFYESEVEQGGATVF--PVLDLTLWPQKGSAVFWFNLYRNGEGNEDTRHAAXPVLKGTKXVSNFWIHERGQEFTRPCALTP 534 HSP 2 Score: 88.9669 bits (219), Expect = 3.324e-18 Identity = 66/249 (26.51%), Postives = 120/249 (48.19%), Query Frame = 0
Query: 277 FRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPP--SRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
+C + P++++ P K E + + P + +F ++ + E++ + + P R ++ ++N + E ++ R ++KT W+ + E+ V+ G+ Q++ T LN+ +S + QV+NYG+ G H DPH E S LG +G+ AT + + + GGAT V+P ++ +WP KG+A +W++L G N T H P+LKG+K + N WI
Sbjct: 311 LKCFLDFGKHPFMKIAPIKAEKLYSKPDIYIFHNVISDNEIETIKNMANP---RFKRATVQN-AKTGELETANYR--ISKTA--WIK--------------------SEENDVVKGIYQRVHDVTGLNME---TSEELQVSNYGIGG----HYDPHFDFARREEKDAFSSLG---TGNRIATWLFYESEVEQGGAT--VFPVLDLTLWPQKGSAVFWFNLYRNGEGNEDTRHAAXPVLKGTKXVSNFWI 519 HSP 3 Score: 87.4261 bits (215), Expect = 1.180e-17 Identity = 138/561 (24.60%), Postives = 224/561 (39.93%), Query Frame = 0
Query: 528 QSTEALIRSE---IQTVKSMRELQNNLAQLSQALEEKELVTPPVKEAITVKKV------------QISNL------------MATFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQP-MGADDTLTLAFEAFKIG-------WYDHAANLTRFTREHLSLAS---------FSPHQRIHLER---FTKDLVR---THNELLRRK---KTIVGEGFKLLPYLVDGNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEKKEDV-MRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWI 1034
Q E I+ E IQ +K E N + EE + V+ P+ I +KK+ + SNL +P ++ + A L+ + + Y+I + GR+ V++ P + A D L + + G W A L+R+ E S FS + + HL R FT L++ H + K + + E P L DG+ +K LK ED ++ +E E V +R+CRGE E K R +C +PF+++AP K E L P I H +++++EI+ + + + P R + ++ K + RI + WI+ + D VK GI++ +H ++ NM +E + QV+NYG+GG H DPH + E L TG+ +AT++ + ++V+ GG T F + T+ P +GSA FW NL + + H PVL G+K V + WI
Sbjct: 29 QXVEKYIKEEESRIQRLKGYVEQYNKYLGSADGHEE-DFVSNPLNSFILIKKLTSDFNDVYDLIKETSNLNKFSENITDSRESNKWPTDEDLKGAASALIRLQEVYKINTRDMRN----------GRLNGVDYGPQLTAHDCFELGRQTYNEGDHYRTEIWMTEA--LSRYDEEDEKTVSKGDIYEYLGFSAYVQGHLRRALKFTNLLLKELPNHPRAIGNKLYYENAIMESGGSGPSL-DGSQRKGDDGLDELK-EDAVLKEGTGSNAEELLYPETVRYQRLCRGE--SNEKKKFDRDLKCFLDFGKHPFMKIAPIKAEKLYSKPDIYIFHNVISDNEIETIKNMANPRFKR------ATVQNAKTGELETANYRI---SKTAWIK----SEENDVVK---GIYQ----------------RVHDVT-----GLNMETSE-----ELQVSNYGIGG----HYDPHFDFARREEKDAFSSL-GTGNRIATWLFYESEVEQGGATVFPV--LDLTLWPQKGSAVFWFNLYRNGEGNEDTRHAAXPVLKGTK----XVSNFWI 519
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Match: EMLSAG00000012938 (supercontig:LSalAtl2s:LSalAtl2s988:173248:175333:-1 gene:EMLSAG00000012938 transcript:EMLSAT00000012938 description:"snap_masked-LSalAtl2s988-processed-gene-1.22") HSP 1 Score: 93.2041 bits (230), Expect = 1.276e-19 Identity = 109/406 (26.85%), Postives = 176/406 (43.35%), Query Frame = 0
Query: 94 FPDRIDYDGAVYAMVVLKDTYDFDVFQ-AAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFL-----KGVFEVIATLPEAQRPVMSKYE-SMRRSLVKVNNEYITKKMIMAG-------ANYKVLPYVVDANLERKKKQPKVLRE----NQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPPPRFR---CAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLR-----KSGDMFATIMGWIQDEPVGGATAFV 473
P D DGA A+V L + Y+F++ +G+I+ + +G+ R E+ +L D+ K+ M + I+ + K + + T P P + ++ +++VK +K+ G NY +PY D L +KKK KV+ E N ++ D +Q ++Y R +CNG RS P + C Y H PY R GP K+E VS P++V+ D + + + ++P+L R+ KN + RR+ + Q W+++ V +K+ I+D N ++S + S YQV NYG AG +H D L G P+ L R K+GD ATIMG++ D GG T +
Sbjct: 150 LPGAEDLDGASTALVRLHEYYNFNITSFVEEGVIETK---DGE--RFETTGELSVWDIFKIGVKGTNMMILGSGIEIMSHGLAKAKRDGLVTTP----PFVDSHDFKTLKNIVKTAKTVHDQKLDRWGPLTPTHSTNY--VPY--DKRLAKKKKFNKVVSEKMILNNPSIIDT-IQETKQY--RKLCNGEELRS----PDVLKDLTCRYEHRGLPYYRYGPHKIETVSLRPHIVIIHDFISDEITKEFINSASPNLKRSAMRGFKNGTN-----AVDDRRV---SEQSWINEEVPAARKL-TDQINDYLNLLVDSSI--------------------HSELYQVANYGTAGHYSSHYDQ--VLMGSS--PNDLERRNIFNIKTGDRMATIMGYLSDVEEGGYTTHI 502 HSP 2 Score: 87.0409 bits (214), Expect = 1.067e-17 Identity = 100/398 (25.13%), Postives = 163/398 (40.95%), Query Frame = 0
Query: 1121 DFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDR-----LKVVIKQSQNHY--LTTRRAPFGQDYRV--LPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAE-RRVCRGELVGQK--VKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLF--ASGDIFATFMAWLDDVDAGGGT 1504
D DGA T L+ L E YN + F+ V+ + F++ L+V DI + VK + + M + + A++D P +D LK ++K ++ + R P + +PY D L K KK K V + K + NP+ + ++E + R++C GE + +KD +C + H PY R P K+E + RP +I+HDF+S+ + + P L RS + N N + ++++ + Q+WI V P L+ +I + + S + YQV NYG +G H D + RR +F +GD AT M +L DV+ GG T
Sbjct: 155 DLDGASTALVRLHEYYNFNITSFVEEGVIETK-DGERFETTGELSVWDIFKIGVKGTNMMILGSGIEIM------SHGLAKAKRDGLVTTPPFVDSHDFKTLKNIVKTAKTVHDQKLDRWGPLTPTHSTNYVPY--DKRLAKKKKFNKVVSE----KMILNNPS--IIDTIQETKQYRKLCNGEELRSPDVLKD---LTCRYEHRGLPYYRYGPHKIETVSLRPHIVIIHDFISDEITKEFINSASPNLKRSAMRGFKN---GTNAVDDRRV-----SEQSWINEEV--------------------------PAARKLTDQINDYLNLLVDSSIHSELYQVANYGTAGHYSSHYD-QVLMGSSPNDLERRNIFNIKTGDRMATIMGYLSDVEEGGYT 499 HSP 3 Score: 75.0998 bits (183), Expect = 7.223e-14 Identity = 67/272 (24.63%), Postives = 118/272 (43.38%), Query Frame = 0
Query: 710 LPYLVDGNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEKKEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGA---EIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTA 978
+PY D L KK K + + N P +++ E K+ R++C GE + + + ++ C + H P+ R P K+E + P +I+H+ ++++ + ++ ++PNL R S ++ K+ A RR+ + Q WI + + P KL+ +I N+++ S YQV NYG G SH D + G+ ++ + F TGD +AT M +L+DV+ GG T
Sbjct: 279 VPY--DKRLAKKKKFNKVVSEKMILNN--PSIIDTIQETKQ--YRKLCNGEELRS--PDVLKDLTCRYEHRGLPYYRYGPHKIETVSLRPHIVIIHDFISDEITKEFINSASPNLKR------SAMRGFKNGTNAVDDRRV---SEQSWINEEV-------------------------------PAARKLTDQINDYLNLLVDSSIHSELYQVANYGTAGHYSSHYDQ--VLMGSSPNDLERRNIFNIKTGDRMATIMGYLSDVEEGGYTT 500
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Match: EMLSAG00000010988 (supercontig:LSalAtl2s:LSalAtl2s73:416831:418075:1 gene:EMLSAG00000010988 transcript:EMLSAT00000010988 description:"maker-LSalAtl2s73-augustus-gene-4.34") HSP 1 Score: 63.929 bits (154), Expect = 1.439e-12 Identity = 28/62 (45.16%), Postives = 39/62 (62.90%), Query Frame = 0
Query: 467 GGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
GG TAF +K LV+P KG++ +W++L G + T+HG CP + G KW+ NKWI F Q
Sbjct: 9 GGGTAFTKINK--LVYPKKGSSVFWFNLYSDGKEDTLTIHGACPTMYGIKWVSNKWIRSFAQ 68 HSP 2 Score: 63.5438 bits (153), Expect = 1.891e-12 Identity = 30/71 (42.25%), Postives = 41/71 (57.75%), Query Frame = 0
Query: 1492 MAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQ 1562
M +L DVD GGGT F ++V+P +GS+ FW +L G + + H CP + G KW+ NKWI F Q
Sbjct: 1 MLYLSDVD-GGGTAFTK--INKLVYPKKGSSVFWFNLYSDGKEDTLTIHGACPTMYGIKWVSNKWIRSFAQ 68 HSP 3 Score: 51.2174 bits (121), Expect = 4.946e-8 Identity = 25/59 (42.37%), Postives = 35/59 (59.32%), Query Frame = 0
Query: 965 MAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
M +L+DV GG TAF + V P +GS+ FW NL +D + + L+ HG CP + G K
Sbjct: 1 MLYLSDVDGGG-TAFTK--INKLVYPKKGSSVFWFNLYSDGKEDTLTIHGACPTMYGIK 56
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Match: gi|82082587|sp|Q5ZLK5.1|P4HA2_CHICK (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor) HSP 1 Score: 125.946 bits (315), Expect = 1.382e-28 Identity = 124/471 (26.33%), Postives = 204/471 (43.31%), Query Frame = 0
Query: 1111 LEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNS-------------ALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQP----KFVRQMGPYKRNITNPNEYL-QGDLREEAERRVCRGELVGQKVKDEGPQSC-YHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRR----RLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIY 1558
+++ F E D GA L+ L++TY L + RG L +++S L V D + A Y V +M + L + QF ++ + +I H RA G + R L++ EK + + V Q G Y+R P +YL + D+ E +CRGE V + + C YH N+NP+L IAPFK E P + +D MS+ E+ + + +KP+L+R+ + ++ K L R+ + +W+ ++DD +P++ +++++++ T + + + A QV NYG+ G E H D SRR L + G+ ATF+ ++ DV+AGG T F P F +WP +G+A FW +L G + R+ H CP+L G KW+ NKW +
Sbjct: 118 IQRQFFPTEEDETGAAKALMRLQDTYKLDPETLSRG-----NLPGTKYRS--SLTVSDCFGMGKTAYNDGDYYHTVLWMEQALKQHDEGEDTTVSKVEILDYLSYAVFQFGDLHRAMELTRRLISLDSTH----ERA--GSNLRYFEKLLEKEREKPSNKTVATTEPVVQSGAYER----PLDYLPERDIYEA----LCRGEGVKMTPRRQKRLFCRYHDGNRNPHLLIAPFKEEDEWDSPHIVRYYDVMSDEEIEKIKQLAKPKLARA-----TVRDPKTGVLTVASYRV---SKSSWL-----------EEDD--------------DPVVAKVNQRMQQITGLTVKT---AELLQVANYGMGGQYEPHFD-----------FSRRPFDSTLKSEGNRLATFLNYMSDVEAGGATVF--PDFGAAIWPKKGTAVFWYNLFRSGEGDYRTRHAACPVLVGCKWVSNKWFH 518 HSP 2 Score: 109.383 bits (272), Expect = 2.902e-23 Identity = 128/577 (22.18%), Postives = 229/577 (39.69%), Query Frame = 0
Query: 4 VILSSTIVLLWHGGTGYDLDSGYEFHLFENFGLTKDLFLRE-----------RMFQSTLTEIKAQLELRLKALEHKSTT----------------------LIELESQALTARKNSLNETTAI----FPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYE---SMRRSLVKVNNEY----ITKKMIM-------AGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSAREYY-----LRAVCNGFSFRSYFEPPPRFRCAYLH-HQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
++ ++ L+WH E F + G DL E R ++ L++IK+ E ++ L KST+ +ELE+ L N I FP D GA A++ L+DTY D ++G + K+ + L D M A Y ++ +++ + + E + +SK E + ++ + + + +T+++I AG+N + +++ ER+K K + + + + +Y A+C G + R C Y +++P+L + PFK E +P++V + D+ + +E++ + + P L R + + K+G L+ Y K + DD+ V+ ++Q++++ T L + ++ QV NYG+ G E H D P L+ G+ AT + ++ D GGAT V+P +WP KG A +WY+L G + RT H CP+L G KW+ NKW
Sbjct: 7 LVFFTSAFLIWHA----------EAEFFTSIGQMTDLIYAEKDLVQSLKEYIRAEETKLSQIKSWAE-KMDVLTSKSTSDPEGYLAHPVNAYKLVKRLNTDWLELENLVLQDTTNGFITNLTIQRQFFPTEEDETGAAKALMRLQDTYKLDPETLSRGNLPGTKYRSS----------LTVSDCFGMGKTAYNDGDYYHTVLWME---QALKQHDEGEDTTVSKVEILDYLSYAVFQFGDLHRAMELTRRLISLDSTHERAGSNLRYFEKLLEK--EREKPSNKTVATTEPVVQSGAYERPLDYLPERDIYEALCRGEGVKMTPRRQKRLFCRYHDGNRNPHLLIAPFKEEDEWDSPHIVRYYDVMSDEEIEKIKQLAKPKLAR---------ATVRDPKTGV------------LTVASYRVSKSSWLEEDDD-------PVVAKVNQRMQQITGLTVK---TAELLQVANYGMGGQYEPHFD-------FSRRPFDSTLKSEGNRLATFLNYMSDVEAGGAT--VFPDFGAAIWPKKGTAVFWYNLFRSGEGDYRTRHAACPVLVGCKWVSNKWF 517 HSP 3 Score: 103.99 bits (258), Expect = 1.660e-21 Identity = 115/459 (25.05%), Postives = 188/459 (40.96%), Query Frame = 0
Query: 586 FPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQPMGADDTL--------TLAFEAFKIGWYDHAANLTRFTREHLSLASFSP------HQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDGNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEK------KEDVMRRICRGEIVGAEVKNQVR-FQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
FP E+ AK L+ + DTY++ + L++ + L + R L G T T+ + + +D + T E L S++ H+ + L R L TH G + L++ +K S++ P V + YE+ + D+ +CRGE V + Q R F R H + NP L +APFK E SP + +++++++EI+ + + P L+R P LT VA R + K+ W+E+ D + P + K++++++Q T + + ++ QV NYG+GG E H D P L S G+ LATF+ +++DV+AGG T F G + P +G+A FW NL + + H CPVL G K
Sbjct: 123 FPTEEDETGAAKALMRLQDTYKLDPETLSRGN---LPGTKYRSSLTVSDCFGMGKTAYNDGDYYHTVLWMEQALKQHDEGEDTTVSKVEILDYLSYAVFQFGDLHRAMELTRRLISLDSTHER--------AGSNLRYFEKLLEKEREKPSNKT--------VATTEPVVQSGAYERPLDYLPERDIYEALCRGEGVKMTPRRQKRLFCRYH-DGNRNPHLLIAPFKEEDEWDSPHIVRYYDVMSDEEIEKIKQLAKPKLARATVRDPKTGVLT----VASYR---VSKS--SWLEE-----------DDD-------------------PVVAKVNQRMQQITGLTVK---TAELLQVANYGMGGQYEPHFD-------FSRRPFDSTLKSEGNRLATFLNYMSDVEAGGATVFPDFG--AAIWPKKGTAVFWYNLFRSGEGDYRTRHAACPVLVGCK 510
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Match: gi|2498741|sp|Q60716.1|P4HA2_MOUSE (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor) HSP 1 Score: 120.553 bits (301), Expect = 7.419e-27 Identity = 125/482 (25.93%), Postives = 207/482 (42.95%), Query Frame = 0
Query: 1111 LEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNS-------------ALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEK-LKKQPK--FVRQMGPYKRNITNPNEYL-QGDLREEAERRVCRGELVGQKVKDEGPQSC-YHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPND 1574
+++ F + D GA L+ L++TY L RG+ L ++Q+ L+V D L A + Y V +M +V L + Q ++ + ++ +H RA G + R L+++ K L Q Q Y+R P +YL + D+ E +CRGE V + + C YH N+ P L IAPFK E P + +D MS+ E+ + E +KP+L+R+ + ++ K L R+ + +W+ ++DD +P++ ++R+++ T + + + A QV NYG+ G E H D + + A +R +G+ ATF+ ++ DV+AGG T F P +WP +G+A FW +L G + R+ H CP+L G KW+ NKW + Q CGT D
Sbjct: 120 VQRQFFPTDEDESGAARALMRLQDTYKLDPDTISRGE-----LPGTKYQA--MLSVDDCFGLGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATVTKSLVLDYLSYAVFQLGDLHRAVELTRRLLSLDPSH----ERA--GGNLRYFERLLEEERGKSLSNQTDAGLATQENLYER----PTDYLPERDVYES----LCRGEGVKLTPRRQKKLFCRYHHGNRVPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARA-----TVRDPKTGVLTVASYRV---SKSSWL-----------EEDD--------------DPVVARVNRRMQHITGLTVKT---AELLQVANYGMGGQYEPHFD---FSRSDDEDAFKR--LGTGNRVATFLNYMSDVEAGGATVF--PDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGQEFLRPCGTTEVD 537 HSP 2 Score: 103.605 bits (257), Expect = 2.480e-21 Identity = 113/464 (24.35%), Postives = 191/464 (41.16%), Query Frame = 0
Query: 586 FPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQPM-GADDTLTLAFEAFKIGWYDHA----------------ANLTR-FTREHLSLASF---SPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVD---GNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEKKEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNN--PFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
FP ++ A+ L+ + DTY++ D ++ RG + +Q M DD L A+ G Y H A +T+ ++LS A F H+ + L R L +H G + L++ G + +E+ + R +Y + DV +CRGE V + Q + C + HH N P L +APFK E SP + +++++++EI+ + + + P L+R P LT VA R + K+ W+E+ DP +R+ +++++ T + + ++ QV NYG+GG E H D + ++ + L TG+ +ATF+ +++DV+AGG T F G + P +G+A FW NL + + H CPVL G K
Sbjct: 125 FPTDEDESGAARALMRLQDTYKLDPDTIS----------RGELPGTKYQAMLSVDDCFGLGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATVTKSLVLDYLSYAVFQLGDLHRAVELTRRLLSLDPSHER--------AGGNLRYFERLLEEERGKSLSNQTDAGLATQENLYER------PTDYLPERDVYESLCRGEGVKLTPRRQKKL-FCRY-HHGNRVPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLT----VASYR---VSKS--SWLEE----------------------DDDPVVARV--------NRRMQHITGLTVK---TAELLQVANYGMGGQYEPHFD----FSRSDDEDAFKRL-GTGNRVATFLNYMSDVEAGGATVFPDLG--AAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCK 513 HSP 3 Score: 95.9005 bits (237), Expect = 5.708e-19 Identity = 73/265 (27.55%), Postives = 121/265 (45.66%), Query Frame = 0
Query: 260 AVCNGFSFRSYFEPPPRFRCAYLH-HQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
++C G + + C Y H ++ P L + PFK E +P++V + D+ + +E++ + + P L R + + K+G L+ Y K + DD+ V+ ++++++ T L + ++ QV NYG+ G E H D S + E RLG +G+ AT + ++ D GGAT V+P +WP KG A +WY+L G + RT H CP+L G KW+ NKW
Sbjct: 294 SLCRGEGVKLTPRRQKKLFCRYHHGNRVPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLAR---------ATVRDPKTGV------------LTVASYRVSKSSWLEEDDD-------PVVARVNRRMQHITGLTVK---TAELLQVANYGMGGQYEPHFDFSRSDD--EDAFKRLG---TGNRVATFLNYMSDVEAGGAT--VFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWF 520
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Match: gi|20455169|sp|O15460.1|P4HA2_HUMAN (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor) HSP 1 Score: 119.783 bits (299), Expect = 1.469e-26 Identity = 125/480 (26.04%), Postives = 209/480 (43.54%), Query Frame = 0
Query: 1111 LEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHY----LTTRRAPF-------GQDYRVLPYLVDDNLEKL---KKQPKFVRQMGPYKRNITNPNEYL-QGDLREEAERRVCRGELVGQKVKDEGPQSC-YHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPND 1574
+++ F + D GA L+ L++TY L RG+ L ++Q+ L+V D + A + Y V +M +V E+ K Q +D L + Q + + LT R G + R L+++ EK + + + G Y+R P +YL + D+ E +CRGE V + + C YH N+ P L IAPFK E P + +D MS+ E+ + E +KP+L+R+ + ++ K L R+ + +W+ ++DD +P++ ++R+++ T + + + A QV NYG+ G E H D + E + +G+ ATF+ ++ DV+AGG T F P +WP +G+A FW +L G + R+ H CP+L G KW+ NKW + Q CG+ D
Sbjct: 118 VQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRGE-----LPGTKYQA--MLSVDDCFGMGRSAYNEGDYYHTVLWMEQVLKQ---LDAGEEATTTKSQV-LDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYER----PVDYLPERDVYES----LCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARA-----TVRDPKTGVLTVASYRV---SKSSWL-----------EEDD--------------DPVVARVNRRMQHITGLTVKT---AELLQVANYGVGGQYEPHFD---FSRNDERDTFKH--LGTGNRVATFLNYMSDVEAGGATVF--PDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGQEFLRPCGSTEVD 535 HSP 2 Score: 102.064 bits (253), Expect = 6.660e-21 Identity = 117/475 (24.63%), Postives = 191/475 (40.21%), Query Frame = 0
Query: 579 ISNLMAT---FPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQPM-GADDTLTLAFEAFKIGWYDH-----------------AANLTRFTREHLSLASF---SPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDGNLKK---KSSQPKFLKREDFFNRDPPEVLNQEYEKKEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNN--PFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLH-STGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
I+NL FP ++ + AK L+ + DTYR+ ++ RG + +Q M DD + A+ G Y H A ++LS A F H+ + L R L +H G + L++ +K ++ + E + R +Y + DV +CRGE V + Q R C + HH N P L +APFK E SP + +++++++EI+ + + + P L+R P LT VA R + K+ W+E+ DP +R+ +++++ T + + ++ QV NYG+GG E H D + F H TG+ +ATF+ +++DV+AGG T F G + P +G+A FW NL + + H CPVL G K
Sbjct: 113 IANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTIS----------RGELPGTKYQAMLSVDDCFGMGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATTTKSQVLDYLSYAVFQLGDLHRALELTRRLLSLDPSHER--------AGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPV------DYLPERDVYESLCRGEGVKLTPRRQKRL-FCRY-HHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLT----VASYR---VSKS--SWLEE----------------------DDDPVVARV--------NRRMQHITGLTVK---TAELLQVANYGVGGQYEPHFD------FSRNDERDTFKHLGTGNRVATFLNYMSDVEAGGATVFPDLG--AAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCK 511 HSP 3 Score: 95.1301 bits (235), Expect = 1.018e-18 Identity = 100/446 (22.42%), Postives = 187/446 (41.93%), Query Frame = 0
Query: 93 IFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYESMRRSLVKVNNEY----ITKKMIM-------AGANYKVLPYVVDANLER---KKKQPKVLRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPPPRFRCAYLH-HQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
FP D GA A++ L+DTY D ++G + K+ L D M A + Y ++ +++ V + + EA + + ++ ++ + + +T++++ AG N + +++ E+ + + ++ + + R+ Y ++C G + R C Y H ++ P L + PFK E +P++V + D+ + +E++ + + P L R + + K+G L+ Y K + DD+ V+ ++++++ T L + ++ QV NYG+ G E H D + E LG +G+ AT + ++ D GGAT V+P +WP KG A +WY+L G + RT H CP+L G KW+ NKW
Sbjct: 122 FFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKYQA----------MLSVDDCFGMGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATTTKSQVLDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPERDVY-ESLCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLAR---------ATVRDPKTGV------------LTVASYRVSKSSWLEEDDD-------PVVARVNRRMQHITGLTVK---TAELLQVANYGVGGQYEPHFDFSRNDERDTF--KHLG---TGNRVATFLNYMSDVEAGGAT--VFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWF 518
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Match: gi|81870817|sp|Q6W3F0.1|P4HA3_MOUSE (RecName: Full=Prolyl 4-hydroxylase subunit alpha-3; Short=4-PH alpha-3; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3; Flags: Precursor) HSP 1 Score: 118.627 bits (296), Expect = 3.424e-26 Identity = 128/494 (25.91%), Postives = 204/494 (41.30%), Query Frame = 0
Query: 1101 KFELTEKLKPL-------EKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRG--KVVTNGLIPQEFQSP---YHLNVQDILFLAVKANEKRWYDAAVPFMRE---VFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRG---ELVGQKVKDEGPQ-SCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQT-WIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPND 1574
E TE ++ L E+D E D +GA L+ L++ Y L +K RG + VT I + SP + L D + A + Y A+P++ E +F A E + A + +D L Q N + +++ V DN + K+ R + + + ++ R G L Q + P C + N +PYL + P + EV+ RP + HDF+S+ E + E ++P L RS V ++ E K++++ + +++ W+ V +PMLV+L +I T + + + A QV NYG+ G H +PH + P+S SG+ ATFM +L V+AGG T F F V + +A FW +L G + + H GCP+L G KW+ NKWI+ + Q C T P D
Sbjct: 104 SLEATENIRALKDGYEKVEQDLPAFE-DLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSSI-TDLYSPRQLFSLTADDCFQVGKVAYDTGDYYHAIPWLEEAVSLFRRAHGEWKTEDE--ASLEDALDYLAFACFQVGN---VSCALSLSREFLVYS---PDNKRMARNVLKYERLLAENGHQMAAETAIQRPNVPHLQTRDTYEGLCQTLGSQPTHYQIPSLYCSYETNSSPYLLLQPARKEVVHLRPLIALYHDFVSDEEAQKIRELAEPWLQRS---VVASGE--------KQLQVEYRISKSAWLKDTV-------------------------DPMLVTLDHRIAALTGLDIQPPY-AEYLQVVNYGIGG----HYEPH--FDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYGNFSVPV--VKNAALFWWNLHRSGEGDGDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSTNPED 542 HSP 2 Score: 110.923 bits (276), Expect = 9.508e-24 Identity = 120/478 (25.10%), Postives = 194/478 (40.59%), Query Frame = 0
Query: 77 SQALTARKNSLNETTAIFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALK--KMWYDTSIKFLKGVFEVIATLPEAQ---RPVMSKYESMRRSLVKVNNEYITKKMIMAG-------ANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSAREYYLR--------------AVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
++ + A K+ + P D +GA A++ L+D Y +V A+G+ ++ + SP +L AD + K+ YDT + I L EA R ++++ + ++ +Y+ G + + L Y D K+ VL+ ++ LA+ G Q A E ++ +C + P C+Y + PYL L P + E+V P + ++ D + +E + + P L R+ + + Q+ EY+ K WL D V +L L ++ T L+I Y+ QV NYG+ G E H D S P S L KSG+ AT M ++ GGATAF+Y + V V K AA +W++L G + T+H GCP+L G KW+ NKWI + Q
Sbjct: 108 TENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVF--QRVTGSSITDLYSPRQL----FSLTADDCFQVGKVAYDTG-----DYYHAIPWLEEAVSLFRRAHGEWKTEDEASLEDALDYLAFACFQVGNVSCALSLSREFLVYSPD----NKRMARNVLKYERL-LAENGHQMAAETAIQRPNVPHLQTRDTYEGLCQTLGSQPTHYQIPSLYCSYETNSSPYLLLQPARKEVVHLRPLIALYHDFVSDEEAQKIRELAEPWLQRSVVASGEKQLQV-EYRISKSA---------WLKDTV--------------------DPMLVTLDHRIAALTGLDIQPPYAE-YLQVVNYGIGGHYEPHFDHATS------PSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYGNFSVPV--VKNAALFWWNLHRSGEGDGDTLHAGCPVLVGDKWVANKWIHEYGQ 530 HSP 3 Score: 78.1814 bits (191), Expect = 2.431e-13 Identity = 66/260 (25.38%), Postives = 114/260 (43.85%), Query Frame = 0
Query: 775 CHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWI 1034
C + +++P+L L P + EV+ P + H+ ++++E Q + + + P L R ++ +L R++ W++D T+D P + L +I T + + ++ QV NYG+GG H +PH ++ A P + +G+ +ATFM +L+ V+AGG TAF + V+ + +A FW NL + + H GCPVL G K V + WI
Sbjct: 320 CSYETNSSPYLLLQPARKEVVHLRPLIALYHDFVSDEEAQKIRELAEPWLQRSV-VASGEKQLQVEYRISKS----------AWLKD-----TVD-------------------------PMLVTLDHRIAALTGLDIQPPYAEY-LQVVNYGIGG----HYEPH--FDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYGNFSVPVV--KNAALFWWNLHRSGEGDGDTLHAGCPVLVGDKW----VANKWI 525
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Match: gi|32171589|sp|Q20065.1|P4HA2_CAEEL (RecName: Full=Prolyl 4-hydroxylase subunit alpha-2; Short=4-PH alpha-2; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Flags: Precursor) HSP 1 Score: 117.857 bits (294), Expect = 6.713e-26 Identity = 114/451 (25.28%), Postives = 194/451 (43.02%), Query Frame = 0
Query: 1119 EVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCG 1569
E D GA T LL L++TY+L D ++NG+I E S L+ D + A ++ Y + +M+ +P +++ + L + LT R A ++ V E + + V + P I N G + +A +CRGE+ + K + CY L P+L++AP K+E+L P ++ + + ++E+ + E + P+L R+ + + K L++ RI + W+ D +P++ ++R+IE T L++ ++ + QV NYGL G H DPH E + + L +G+ AT + ++ + GG T F G V+P++ A FW +LR G +LR+ H CP+L G KW+ NKWI+ Q CG
Sbjct: 125 EEDLSGAATALLRLQDTYSLDTLD------LSNGIIGGEKVS-NKLSGHDTFEVGRSAYNQKDYYHCLMWMQVALVKIENENPPTIEEWEILEYLAYSLYQQGNVRRALSLTKRLAKIAPNHPRAKGNVK-WYEDMLQGKDMVGDLPP----IVNKRVEYDGIVERDAYEALCRGEIPPVEPKWKNKLRCY-LKRDKPFLKLAPIKVEILRFDPLAVLFKNVIHDSEIEVIKELASPKLKRA-----TVQNSKTGELEHATYRI---SKSAWLKG-------------------------DLDPVIDRVNRRIEDFTN---LNQATSEELQVANYGLGG----HYDPHFDFARKEEKNAFKTL-NTGNRIATVLFYMSQPERGGATVFNHLG--TAVFPSKNDALFWYNLRRDGEGDLRTRHAACPVLLGVKWVSNKWIHEKGQEFTRPCG 519 HSP 2 Score: 114.775 bits (286), Expect = 5.419e-25 Identity = 123/492 (25.00%), Postives = 201/492 (40.85%), Query Frame = 0
Query: 54 IKAQLELRLKALEHKSTTLIELESQALTARKNSLNE---TTAIFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYV-----VDANLERKKKQPKVLRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEP------PP-------RFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQH-WLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
I A L ++ E K I L ++A T KN + + FP D GA A++ L+DTY D + G+I EK +N KL D ++ A + Y + +++ + + P + ++E + EY+ + G + L + N R K K E+ + DM R +G R +E PP + RC YL P+L+L P KVE++ +P V+F+++ E++ + ++P L R + K+G+ + + WL D+D V+ +++++E T LN + +S + QV NYGL G H DPH E + +G+ AT++ ++ GGAT V+ H V+P+K A +WY+L G + RT H CP+L G KW+ NKWI
Sbjct: 79 INAYLLIKRLTTEWKKVENIMLNNKASTFLKNITDNRVRSEVKFPGEEDLSGAATALLRLQDTYSLDTLDLSNGIIGGEKVSN----------KLSGHDTFEVGRSAYNQKDYYHCLMWMQ---VALVKIENENPPTIEEWEIL---------EYLAYSLYQQGNVRRALSLTKRLAKIAPNHPRAKGNVK-WYEDMLQGKDMVGDLPPIVNKRVEYDGIVERDAYEALCRGEIPPVEPKWKNKLRC-YLKRDKPFLKLAPIKVEILRFDPLAVLFKNVIHDSEIEVIKELASPKLKR---------ATVQNSKTGELEHATYRISKSAWLKG-----------DLD---------PVIDRVNRRIEDFTNLN---QATSEELQVANYGLGG----HYDPHFDFARKE-EKNAFKTLNTGNRIATVLFYMSQPERGGAT--VFNHLGTAVFPSKNDALFWYNLRRDGEGDLRTRHAACPVLLGVKWVSNKWI 507 HSP 3 Score: 107.842 bits (268), Expect = 9.584e-23 Identity = 128/507 (25.25%), Postives = 213/507 (42.01%), Query Frame = 0
Query: 562 ELVTPPVKEAITVKKV-----QISNLM--------------------ATFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQPMGADDTLTLAFEAFKIGWYDHAANLTRFTREHLSLASFSP---HQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPY--LVDGNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEK----KEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWI 1034
E VT P+ + +K++ ++ N+M FP E+ + A LL + DTY +D L D + + G ++ N + DT + A+ Y H L + + + +P + LE L + N +RR ++ K+ P GN+K L+ +D PP ++N+ E + D +CRGEI E K + + RC +L + PFL+LAP K+E+L P ++ ++++ EI+ + + ++P L R TVQ + +AT + S+ W +GD P I +++++IE TN+ + +S + QV NYGLGG H DPH + E + L +TG+ +AT + +++ + GG T F+ G V P++ A FW NL D + + H CPVL G K V + WI
Sbjct: 73 EFVTNPINAYLLIKRLTTEWKKVENIMLNNKASTFLKNITDNRVRSEVKFPGEEDLSGAATALLRLQDTY--SLDTL-----DLSNGIIGGEKVSNK--LSGHDTFEVGRSAYNQKDYYHC--LMWMQVALVKIENENPPTIEEWEILEYLAYSLYQQGN--VRRALSLTKRLAKIAPNHPRAKGNVKWYED---MLQGKDMVGDLPP-IVNKRVEYDGIVERDAYEALCRGEIPPVEPKWKNKL-RC-YLKRDKPFLKLAPIKVEILRFDPLAVLFKNVIHDSEIEVIKELASPKLKR--------------------------ATVQNSKTGELEHATY---RISKSAW----LKGD------LDPVIDRVNRRIEDFTNL---NQATSEELQVANYGLGG----HYDPHFDFARKEEKNAFKTL-NTGNRIATVLFYMSQPERGGATVFNHLG--TAVFPSKNDALFWYNLRRDGEGDLRTRHAACPVLLGVKW----VSNKWI 507
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Match: gi|74738714|sp|Q7Z4N8.1|P4HA3_HUMAN (RecName: Full=Prolyl 4-hydroxylase subunit alpha-3; Short=4-PH alpha-3; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3; Flags: Precursor) HSP 1 Score: 115.161 bits (287), Expect = 5.120e-25 Identity = 113/467 (24.20%), Postives = 192/467 (41.11%), Query Frame = 0
Query: 77 SQALTARKNSLNETTAIFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKL------DYKDLKK----MADVALKKMWYDTSIKFLKGVF-----EVIATLPEAQRPVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
S+ + A K+ + P D +GA A++ L+D Y +V A+G+ ++ + SP +L D + K M D W + ++ +G + E A+L +A + Y R V + ++ + N ++ V+ + V+ E + ++ R+ Y +C + P C+Y + + YL L P + E++ PY+ ++ D + E + + P L R+ + + Q+ EY+ K WL D T++ K L L+ ++ T L++ Y+ QV NYG+ G E H D S P S L KSG+ AT M ++ GGATAF+Y + V V + AA +W++L G + T+H GCP+L G KW+ NKWI + Q
Sbjct: 110 SENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVF--QRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAY--FRAGNVSCALSLSREFLLYSPDNKRMARNVLKYERLLAESPNHVVAEAVIQRPNIPHLQTRDTY-EGLCQTLGSQPTLYQIPSLYCSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRSVVASGEKQLQV-EYRISKSA---------WLKD-------------------TVDPK-LVTLNHRIAALTGLDVRPPYAE-YLQVVNYGIGGHYEPHFDHATS------PSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYANLSVPV--VRNAALFWWNLHRSGEGDSDTLHAGCPVLVGDKWVANKWIHEYGQ 532 HSP 2 Score: 112.464 bits (280), Expect = 3.463e-24 Identity = 118/482 (24.48%), Postives = 200/482 (41.49%), Query Frame = 0
Query: 1104 LTEKLKPLEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRG--KVVTNGLIPQEFQSP---YHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSAL-TFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRG---ELVGQKVKDEGPQ-SCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQT-WIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPND 1574
L + + +E+D E D +GA L+ L++ Y L +K RG + VT I + SP + L D + A + Y A+P++ E + ++ + + A + +D L ++ N + R DN + K+ R + ++ + ++ R G L Q + P C + N N YL + P + EV+ P+ + HDF+S++E + E ++P L RS V ++ E K++++ + +++ W+ V +P LV+L+ +I T + + + A QV NYG+ G H +PH + P+S SG+ ATFM +L V+AGG T F V R +A FW +L G + + H GCP+L G KW+ NKWI+ + Q C + P D
Sbjct: 116 LKDGYEKVEQDLPAFE-DLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAI-TDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLS------REFLLYSPDNKRMARNVLKYERLLAESPNHVVAEAVIQRPNIPHLQTRDTYEGLCQTLGSQPTLYQIPSLYCSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRS---VVASGE--------KQLQVEYRISKSAWLKDTV-------------------------DPKLVTLNHRIAALTGLDVRPPY-AEYLQVVNYGIGG----HYEPH--FDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYANLSVPV--VRNAALFWWNLHRSGEGDSDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSSSPED 544 HSP 3 Score: 79.337 bits (194), Expect = 1.140e-13 Identity = 68/261 (26.05%), Postives = 115/261 (44.06%), Query Frame = 0
Query: 775 CHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIP-TRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWI 1034
C + ++N +L L P + EV+ P+ + H+ +++ E Q + + + P L R ++ +L R++ W++D T+D P + L+ +I T + + ++ QV NYG+GG H +PH ++ A P + +G+ +ATFM +L+ V+AGG TAF Y +P R +A FW NL ++ + H GCPVL G K V + WI
Sbjct: 322 CSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEAQKIRELAEPWLQRSV-VASGEKQLQVEYRISKS----------AWLKD-----TVD-------------------------PKLVTLNHRIAALTGLDVRPPYAEY-LQVVNYGIGG----HYEPH--FDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFI---YANLSVPVVRNAALFWWNLHRSGEGDSDTLHAGCPVLVGDKW----VANKWI 527
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Match: gi|81870816|sp|Q6W3E9.1|P4HA3_RAT (RecName: Full=Prolyl 4-hydroxylase subunit alpha-3; Short=4-PH alpha-3; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3; Flags: Precursor) HSP 1 Score: 114.775 bits (286), Expect = 7.192e-25 Identity = 123/479 (25.68%), Postives = 204/479 (42.59%), Query Frame = 0
Query: 1111 LEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRG--KVVTNGLIPQEFQSP---YHLNVQDILFLAVKANEKRWYDAAVPFMREVFNS-ALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMG------PYKRNITNPN-EYLQGDLREEAERRVCRGELVGQKVKDEGPQ-SCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQT-WIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPND 1574
+E+D E D +GA L+ L++ Y L +K +G + VT I + SP + L D + A + Y A+P++ E + ++ + + A + +D L Q N + +++ V DN + K+ R + + I PN +LQ + +C+ L Q + P C + N +PYL + P + EV+ RP + HDF+S+ E + E ++P L RS V ++ E K++++ + +++ W+ V +P+LV+L R+I T + + + A QV NYG+ G H +PH + P+S SG+ AT M +L V+AGG T F F V + +A FW +L G + + H GCP+L G KW+ NKWI+ + Q C T P D
Sbjct: 123 VEQDLPAFE-DLEGAARALMRLQDAYMLNVKGLAQGVFQRVTGSSI-TDLYSPRQLFSLTADDCFQVGKVAYDTGDYYHAIPWLEEAVSLFRRSYGEWKTEDEASLEDALDYLAFACYQVGN---VSCALSLSREFLVYS---PDNKRMARNVLKYERLLAENGHLMAAETAIQRPNVPHLQ---TRDTYEGLCQ-TLGSQPTHYQIPSLYCSYETNSSPYLLLQPARKEVIHLRPLVALYHDFVSDEEAQKIRELAEPWLQRS---VVASGE--------KQLQVEYRISKSAWLKDTV-------------------------DPVLVTLDRRIAALTGLDIQPPY-AEYLQVVNYGIGG----HYEPH--FDHATSPSSPLYKMKSGNRAATLMIYLSSVEAGGATAFIYGNFSVPV--VKNAALFWWNLHRSGEGDDDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCDTNPED 544 HSP 2 Score: 114.005 bits (284), Expect = 1.155e-24 Identity = 122/478 (25.52%), Postives = 193/478 (40.38%), Query Frame = 0
Query: 77 SQALTARKNSLNETTAIFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALK--KMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYESMRRSLVKVNNEYITKKMI--MAGANYKVLPYVVDANLERK---------------KKQPKVLREN-QVTLADMGVQSAREYYLR------AVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
++ + A K+ + P D +GA A++ L+D Y +V AQG+ ++ + SP +L AD + K+ YDT + I L EA Y K +E + + +A A Y+V +L R+ K ++L EN + A+ +Q +L+ +C + P C+Y + PYL L P + E++ P + ++ D + +E + + P L R+ + + Q+ EY+ K WL D V VL L +++ T L+I Y+ QV NYG+ G E H D S P S L KSG+ AT+M ++ GGATAF+Y + V V K AA +W++L G + T+H GCP+L G KW+ NKWI + Q
Sbjct: 110 TENIRAPKDGYEKVEQDLPAFEDLEGAARALMRLQDAYMLNVKGLAQGVF--QRVTGSSITDLYSPRQL----FSLTADDCFQVGKVAYDTG-----DYYHAIPWLEEAVSLFRRSYGEW-----KTEDEASLEDALDYLAFACYQVGNVSCALSLSREFLVYSPDNKRMARNVLKYERLLAENGHLMAAETAIQRPNVPHLQTRDTYEGLCQTLGSQPTHYQIPSLYCSYETNSSPYLLLQPARKEVIHLRPLVALYHDFVSDEEAQKIRELAEPWLQRSVVASGEKQLQV-EYRISKSA---------WLKDTV--------------------DPVLVTLDRRIAALTGLDIQPPYAE-YLQVVNYGIGGHYEPHFDHATS------PSSPLYKMKSGNRAATLMIYLSSVEAGGATAFIYGNFSVPV--VKNAALFWWNLHRSGEGDDDTLHAGCPVLVGDKWVANKWIHEYGQ 532 HSP 3 Score: 76.2554 bits (186), Expect = 1.161e-12 Identity = 63/260 (24.23%), Postives = 111/260 (42.69%), Query Frame = 0
Query: 775 CHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWI 1034
C + +++P+L L P + EV+ P + H+ ++++E Q + + + P L R ++ +L R++ W++D + P + L ++I T + + ++ QV NYG+GG H +PH ++ A P + +G+ AT M +L+ V+AGG TAF + V+ + +A FW NL + + H GCPVL G K V + WI
Sbjct: 322 CSYETNSSPYLLLQPARKEVIHLRPLVALYHDFVSDEEAQKIRELAEPWLQRSV-VASGEKQLQVEYRISKS----------AWLKDTV------------------------------DPVLVTLDRRIAALTGLDIQPPYAEY-LQVVNYGIGG----HYEPH--FDHATSPSSPLYKMKSGNRAATLMIYLSSVEAGGATAFIYGNFSVPVV--KNAALFWWNLHRSGEGDDDTLHAGCPVLVGDKW----VANKWI 527
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Match: gi|90111077|sp|P54001.2|P4HA1_RAT (RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; Flags: Precursor) HSP 1 Score: 112.849 bits (281), Expect = 2.157e-24 Identity = 80/280 (28.57%), Postives = 130/280 (46.43%), Query Frame = 0
Query: 1290 VCRGELVGQKVKDEGPQSC-YHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKC 1568
+CRGE + + + C YH N+NP +AP K E +P + HD +S+AE+ + + +KPRLSR+ + + + L + R+ + W L Y D P++ ++ +I+ T + + +A + QV NYG+ G E H D + P + R L +G+ AT++ ++ DV AGG T F P VWP +G+A FW +L G + + H CP+L G+KW+ NKW++ Q C
Sbjct: 292 LCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLSRA-----TVHDPETGKLTTAQYRV---SKSAW-----------------------LSGYED--PVVSRINMRIQDLTGLDV---STAEELQVANYGVGGQYEPHFD----FARKDEPDAFREL-GTGNRIATWLFYMSDVSAGGATVF--PEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPC 528 HSP 2 Score: 97.8265 bits (242), Expect = 1.525e-19 Identity = 111/478 (23.22%), Postives = 195/478 (40.79%), Query Frame = 0
Query: 62 LKALEHKSTTLIELESQALTARKNSLNETTAI----FPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLK----GVFEVIATLPEAQRPVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQ----SAREYYLRAVCNGFSFRSYFEPPPRFRCAYLH-HQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGK---KRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
K ++ +T ELE+ L + I FP+ D GA A+ L+DTY+ D ++G ++ + L E +L K AD ++W + ++ L+ + ++ L V + + + L+ + + A N Y++ + K + + T G+ R+ Y +C G + R C Y +++P L P K E P ++ F D+ + E++ + + P L+R +H+ ++GK + V+K+ WLS YED V+ ++ +++ T L+++ ++ + QV NYG+ G E H D E P +G+ AT + ++ D GGAT V+P VWP KG A +WY+L G + T H CP+L G+KW+ NKW+
Sbjct: 85 FKLMKRLNTEWSELENLILKDMSDGFISNLTIQRQYFPNDEDQVGAAKALFRLQDTYNLDTNTISKG--NLPGVKHKSFLTAEDCFELG-KVAYTEADYYHTELWMEQALMQLEEGEMSTVDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLVYFEYIMSKEKDANKSASGDQSDQKTTPKKKGIAVDYLPERQKY-EMLCRGEGIKMTPRRQKRLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLSR---------ATVHDPETGKLTTAQYRVSKSA--WLSG--YEDP------------------VVSRINMRIQDLTGLDVS---TAEELQVANYGVGGQYEPHFDFARKDE-----PDAFRELGTGNRIATWLFYMSDVSAGGAT--VFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWL 517 HSP 3 Score: 93.9745 bits (232), Expect = 2.462e-18 Identity = 78/269 (29.00%), Postives = 123/269 (45.72%), Query Frame = 0
Query: 756 ICRGEIVGAEVKNQVR-FQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
+CRGE + + Q R F R H + N F+ LAP K E P + H+I+++ EI+ + D + P LSR T HD GK ++ V +A L +D P + +++ +I+ T + ++ ++ + QV NYG+GG E H D + + P R L TG+ +AT++ +++DV AGG T F G +V P +G+A FW NL + + H CPVL G+K
Sbjct: 292 LCRGEGIKMTPRRQKRLFCRYHDGNRNPKFI-LAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLSR----------ATVHDPETGKLTTAQYR--------VSKSAWLSGYED---------------------PVVSRINMRIQDLTGLDVS---TAEELQVANYGVGGQYEPHFD----FARKDEPDAFREL-GTGNRIATWLFYMSDVSAGGATVFPEVG--ASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNK 510
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Match: gi|75053350|sp|Q75UG4.1|P4HA3_BOVIN (RecName: Full=Prolyl 4-hydroxylase subunit alpha-3; Short=4-PH alpha-3; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3; Flags: Precursor) HSP 1 Score: 112.079 bits (279), Expect = 5.313e-24 Identity = 113/468 (24.15%), Postives = 189/468 (40.38%), Query Frame = 0
Query: 77 SQALTARKNSLNETTAIFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKL------DYKDLKK----MADVALKKMWYDTSIKFLKGVF-----EVIATLPEAQRPVMSKYESMRRSLVKVNNEYITKKMIM-AGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
S+ + A K+ P D +GA A++ L+D Y +V A+G+ ++ + SP +L D + K M D W + ++ +G + E A+L +A + Y L +N ++++ ++ + N +V V+ + + + E + ++ R+ Y +C + P C+Y PYL L P + E++ PY+V++ D + E + + P L R+ + + + EY+ K WL D V VL L ++ T L++ Y+ QV NYG+ G E H D S P S L SG+ AT M ++ GGATAF+Y + V V K AA +W++L G + T+H CP+L G KW+ NKWI + Q
Sbjct: 110 SENIRALKDGYERVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVF--QRVTGSAVTDLYSPRRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFQAGNVLCALN---LSREFLLYSPDNKRVARNVLKYEKLLAESPNQAVAETVMQRPNVPHLQTRDTY-EGLCQTLGSQPTHYRIPSLYCSYETSSSPYLLLQPVRKEVIHLEPYVVLYHDFVSDAEAQTIRGLAEPWLQRSVVASGEKQLPV-EYRISKSA---------WLKDTV--------------------DPVLVTLDHRIAALTGLDVQPPYAE-YLQVVNYGIGGHYEPHFDHATS------PSSPLYRMNSGNRVATFMIYLSSVEAGGATAFIYGNFSVPV--VKNAALFWWNLHRSGEGDGDTLHAACPVLVGDKWVANKWIHEYGQ 532 HSP 2 Score: 107.071 bits (266), Expect = 1.784e-22 Identity = 121/479 (25.26%), Postives = 196/479 (40.92%), Query Frame = 0
Query: 1104 LTEKLKPLEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRG--KVVTNGLIPQEFQSP---YHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSAL-TFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPN-EYLQGDLREEAERRVCRGELVGQKVKDEGPQ-SCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPND 1574
L + + +E+D E D +GA L+ L++ Y L +K RG + VT + + SP + L D + A + Y A+P++ E + ++ + + A + +D L Q+ N + Y V N+ K +K + + PN +LQ + +C+ L Q P C + + +PYL + P + EV+ P+ ++ HDF+S+AE + ++P L RS V Q ISK+ W+ V +P+LV+L +I T + + + A QV NYG+ G H +PH + P+S SG+ ATFM +L V+AGG T F F V + +A FW +L G + + H CP+L G KW+ NKWI+ + Q C + P D
Sbjct: 116 LKDGYERVEQDLPAFE-DLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAV-TDLYSPRRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFQAGNVLCALNLSREFLLYSPDNKRVARNVLKYEKLLAESPNQAVAETVMQRPNVPHLQ---TRDTYEGLCQ-TLGSQPTHYRIPSLYCSYETSSSPYLLLQPVRKEVIHLEPYVVLYHDFVSDAEAQTIRGLAEPWLQRSV--------VASGEKQLPVEYRISKSA--WLKDTV-------------------------DPVLVTLDHRIAALTGLDVQPPY-AEYLQVVNYGIGG----HYEPH--FDHATSPSSPLYRMNSGNRVATFMIYLSSVEAGGATAFIYGNFSVPV--VKNAALFWWNLHRSGEGDGDTLHAACPVLVGDKWVANKWIHEYGQEFRRPCSSRPED 544 HSP 3 Score: 76.6406 bits (187), Expect = 7.028e-13 Identity = 65/260 (25.00%), Postives = 113/260 (43.46%), Query Frame = 0
Query: 775 CHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWI 1034
C + ++P+L L P + EV+ P+ ++ H+ +++ E Q + + P L R ++ +L R++ W++D T+D P + L +I T + + ++ QV NYG+GG H +PH ++ A P + ++G+ +ATFM +L+ V+AGG TAF + V+ + +A FW NL + + H CPVL G K V + WI
Sbjct: 322 CSYETSSSPYLLLQPVRKEVIHLEPYVVLYHDFVSDAEAQTIRGLAEPWLQRSV-VASGEKQLPVEYRISKS----------AWLKD-----TVD-------------------------PVLVTLDHRIAALTGLDVQPPYAE-YLQVVNYGIGG----HYEPH--FDHATSPSSPLYRMNSGNRVATFMIYLSSVEAGGATAFIYGNFSVPVV--KNAALFWWNLHRSGEGDGDTLHAACPVLVGDKW----VANKWI 527
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Match: gi|129365|sp|P16924.1|P4HA1_CHICK (RecName: Full=Prolyl 4-hydroxylase subunit alpha-1; Short=4-PH alpha-1; AltName: Full=Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1) HSP 1 Score: 111.309 bits (277), Expect = 6.368e-24 Identity = 121/483 (25.05%), Postives = 209/483 (43.27%), Query Frame = 0
Query: 62 LKALEHKSTTLIELESQALTARKNSLNETTAI----FPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLK-------GVFEVIATLPEA--QRPVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVV----DANLERKKKQPKVLRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPPPRFRCAYLH-HQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRI---VTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI 523
K ++ +T ELES L + I FP+ D GA A++ L+DTY+ D ++G ++ + L E +L K AD ++W + ++K L ++ L A Q+ +SK + + L++++ E+ A N K Y++ +AN + + +E +V D + R+Y + +C G + R C Y +++P LGP K E P +V F D+ + +E++ + + P L+R +H+ ++GK V+K+ WLS ES V+ ++ +++ T L+++ ++ + QV NYG+ G E H D G + P +G+ AT + ++ D GGAT V+P VWP KG A +WY+L G + T H CP+L G+KW+ NKW+
Sbjct: 69 FKLMKRLNTEWGELESLVLKDMSDGFISNMTIQRQFFPNDEDQTGARKALLRLQDTYNLDTDTLSRG--NLPGVKHKSFLTAEDCFELG-KIRYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSYAVYQQGDLSKAMMLTKRLLELDPEHQ-----RANGNMKYFEYIMAKEKEANKSSTDAEDQTDKETEVKKKDY-LPERRKYEM--LCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSR---------ATVHDPETGKLTTAHYRVSKSA--WLS--------------------GYESPVVSRINTRIQDLTGLDVS---TAEELQVANYGVGGQYEPHFDF-----GRKDEPDAFKELGTGNRIATWLFYMSDVSAGGAT--VFPEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWL 499 HSP 2 Score: 103.219 bits (256), Expect = 2.776e-21 Identity = 77/287 (26.83%), Postives = 129/287 (44.95%), Query Frame = 0
Query: 1287 ERR----VCRGELVGQKVKDEGPQSC-YHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKC 1568
ERR +CRGE + + + C Y+ N+NP + P K E +P + D +S+ E+ + E +KPRLSR+ + + + L R+ + W+ +P++ ++ +I+ T + + +A + QV NYG+ G E H D + P + + L +G+ AT++ ++ DV AGG T F P VWP +G+A FW +L P G + + H CP+L G+KW+ NKW++ Q C
Sbjct: 267 ERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSRA-----TVHDPETGKLTTAHYRV---SKSAWLSGY-------------------------ESPVVSRINTRIQDLTGLDV---STAEELQVANYGVGGQYEPHFD----FGRKDEPDAFKEL-GTGNRIATWLFYMSDVSAGGATVF--PEVGASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPC 510 HSP 3 Score: 100.138 bits (248), Expect = 2.539e-20 Identity = 109/460 (23.70%), Postives = 186/460 (40.43%), Query Frame = 0
Query: 579 ISNLMAT---FPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQP-MGADDTLTLAFEAFKIGWYDHAANLTRFTREHLSLASFSPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLP--YLVDGNLK--------KKSSQPKFLKREDFFNRDPPEVLNQEYEKKEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLT-KHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
ISN+ FP ++ K LL + DTY + D L+ RG + V H+ + A+D L + Y H + L S ++++ + V +L + + +L P +GN+K +K + ED +++ EV ++Y + +CRGE + + Q R ++ + NP L P K E P + +I++++EI+ + + + P LSR P KLT H RV+ W+ S SP + +++ +I+ T + ++ ++ + QV NYG+GG E H D G + P + TG+ +AT++ +++DV AGG T F G +V P +G+A FW NL + + H CPVL G+K
Sbjct: 95 ISNMTIQRQFFPNDEDQTGARKALLRLQDTYNLDTDTLS----------RGNLPGVKHKSFLTAEDCFELGKIRYTEADYYHTELWMEQALKQLDEGEVSSADKVYILDYLSYAVYQQGDL-SKAMMLTKRLLELDPEHQRANGNMKYFEYIMAKEKEANKSSTDAEDQTDKET-EVKKKDYLPERRKYEMLCRGEGLKMTPRRQKRLFCRYYDGNRNPRYILGPVKQEDEWDKPRIVRFLDIISDEEIETVKELAKPRLSRATVHDPETGKLTTAHYRVSK----------SAWL------------------------------SGYESPVVSRINTRIQDLTGLDVS---TAEELQVANYGVGGQYEPHFDF-----GRKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVG--ASVWPKKGTAVFWYNLFPSGEGDYSTRHAACPVLVGNK 492
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Match: gi|1325270113|ref|XP_023319637.1| (prolyl 4-hydroxylase subunit alpha-2-like [Eurytemora affinis]) HSP 1 Score: 274.633 bits (701), Expect = 1.205e-75 Identity = 158/453 (34.88%), Postives = 252/453 (55.63%), Query Frame = 0
Query: 99 DYDGAVYAMVVLKDTYDFDVFQAAQGLID---IEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQR---PVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSA-------REYYLRAVCNGFSFR-SYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQSTEALIRSE 537
D GA A++ + YD D + +GLID ++ +A+G + +S L +DLK +A ++ K +T IKF++ ++ + ++ V ++E ++ L+K+NN+++T K V Y+VD LE+KK QPK ++ + D+ + ++ E L+ N F+ S + P +C LH DPYL+LGPFK+E+ S P+++V +L T ++ ++L+ +TP L+R+R + + GK R IV K+VQ W+ + Y E D+ + N+T+ + LS ++E A LN+T ++SS YQVTNYGL GLCE+H+D E + P++ R GD ATIM W+ D P GG T+F P V V PTKG+AA+W+DL + R++ T HGGCP+ GSKWI+NKW+F ++Q + R E
Sbjct: 129 DMYGAAKALIFIVHAYDLDTEKFRKGLIDTTDVKYYAHGAKHQGDSA--LTSQDLKVLATMSQKLGLINTGIKFMRSAYKAQEESEDTEQIDVGVKDQFEKAKQDLIKLNNDFVTNKKTFINDLVVVNKYLVDEKLEKKKTQPKFIKSGAIDKVDISLATSFGGDFFRMEALLQGCRNEFNLLPSKIKWPLISKCRLLHQDDPYLKLGPFKMEVASNTPFIMVVHELLTDEDTEYLVNWATPKLSRSRNLDYSKGAYDKDSFKGKVRNIV-KSVQAWMEAVSYH----EPDDMYNPDNFTVNHPRVHKLSARMELAFNLNLTRQWSSNLYQVTNYGLGGLCESHMDAQSFFENMASDPAKYINR--GDYIATIMAWLTDTPAGGGTSFYKPPSSVTVHPTKGSAAFWWDLTHQNRRDQGTKHGGCPVAVGSKWILNKWVFSYNQWDKVPCRLE 572 HSP 2 Score: 262.307 bits (669), Expect = 1.679e-71 Identity = 170/519 (32.76%), Postives = 259/519 (49.90%), Query Frame = 0
Query: 1062 NEINLIQALKHFHRAAAETPQNNEGQISQVSQLQHLARLKFELTEKLKPLEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLI----PQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAE------KDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGE--LVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKC 1568
N NL++ F A + T + + + S +++ L R+ F+ TE LK + E D GA L+ + Y+L + F +G + T + + Q L QD+ LA + + + + FMR + + E KDQF K + + +L N ++T ++ V YLVD+ LEK K QPKF++ K +I+ + R EA + CR E L+ K+K C LH +PYL++ PFKMEV PF M++H+ +++ + +L+ ++ P+LSRSR S K++ + K +R I K+ Q W+ + YH + DD P+ N+T +P + LS ++E A + L ++S+ YQVTNYGL GLCE H+D + E M + + GD AT MAWL D AGGGT F P V PT+GSAAFW DL R+ + H GCP+ GSKWILNKW++ ++Q+ C
Sbjct: 78 NSFNLLKKAAVFIPALSNTLSSLKTKNS--TEMLKLLRILFQETEVLKTIT------EKDMYGAAKALIFIVHAYDLDTEKFRKGLIDTTDVKYYAHGAKHQGDSALTSQDLKVLATMSQKLGLINTGIKFMRSAYKAQEESEDTEQIDVGVKDQFEKAKQDLIKL-------NNDFVTNKKTFINDLVVVNKYLVDEKLEKKKTQPKFIKSGAIDKVDISLATSFGGDFFRMEALLQGCRNEFNLLPSKIKWPLISKCRLLHQDDPYLKLGPFKMEVASNTPFIMVVHELLTDEDTEYLVNWATPKLSRSRNLDYSKGAYDKDSFKGK-VRNIVKSVQAWMEAVSYH-----EPDDMYNPD----NFTVNHPRVHKLSARMELAFNLNLTRQWSSNLYQVTNYGLGGLCESHMDAQSFFENM--ASDPAKYINRGDYIATIMAWLTDTPAGGGTSFYKPPSSVTVHPTKGSAAFWWDLTHQNRRDQGTKHGGCPVAVGSKWILNKWVFSYNQWDKVPC 569 HSP 3 Score: 233.032 bits (593), Expect = 1.644e-61 Identity = 160/513 (31.19%), Postives = 257/513 (50.10%), Query Frame = 0
Query: 527 DQSTEALIRSEIQTVKSMRELQNNLAQLSQALEEKELVTPPVKEAITVKKV--QISNLMATFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQ---PMGADDTLTLAFEAFKIGWYDHAANLTRFT----REHLSLASFSPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDGNLKKKSSQPKFLKRE--DFFNRDPPEVLNQEYEKKEDVMRRICRGE--IVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTL 1026
++S E +R + + +++ + LS L L T E + + ++ Q + ++ T E+ + AK L+ ++ Y + + K D ++ HQ + + D LA + K+G + R E + E+ +DL++ +N+ + KKT + + + YLVD L+KK +QPKF+K D + ++ + E +++ CR E ++ +++K + +C LH ++P+L+L PFKMEV +PF M++HE+L +++ ++L++++TP LSR R + S K D GK R I+ K+VQ W+E V ++ D +P + P +HKLS ++E A N+ LT +WSS YQVTNYGLGGLCESH+D +E P + GD +AT MAWL D AGG T+F TV PT+GSAAFW +L RR+ + HGGCPV GSK L
Sbjct: 66 NKSVEEYVRHPLNSFNLLKKAAVFIPALSNTLSS--LKTKNSTEMLKLLRILFQETEVLKTITEKD-MYGAAKALIFIVHAYDLDTEKFRKGLID-TTDVKYYAHGAKHQGDSALTSQDLKVLATMSQKLGLINTGIKFMRSAYKAQEESEDTEQIDVGVKDQFEKAKQDLIKLNNDFVTNKKTFINDLVVVNKYLVDEKLEKKKTQPKFIKSGAIDKVDISLATSFGGDFFRMEALLQG-CRNEFNLLPSKIKWPL-ISKCRLLHQDDPYLKLGPFKMEVASNTPFIMVVHELLTDEDTEYLVNWATPKLSRSRNLDYSKGAYDK-DSFKGKVRNIV-KSVQAWMEAVSYHEPDDMY--------------NPDNFTVNHPRVHKLSARMELAFNLNLTRQWSSNLYQVTNYGLGGLCESHMDAQSFFENMASDPAKYI--NRGDYIATIMAWLTDTPAGGGTSFYKPPSSVTVHPTKGSAAFWWDLTHQNRRDQGTKHGGCPVAVGSKWIL 554
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Match: gi|1325278729|ref|XP_023324006.1| (prolyl 4-hydroxylase subunit alpha-2-like [Eurytemora affinis]) HSP 1 Score: 273.478 bits (698), Expect = 4.996e-75 Identity = 155/453 (34.22%), Postives = 250/453 (55.19%), Query Frame = 0
Query: 99 DYDGAVYAMVVLKDTYDFDVFQAAQGLID---IEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQRP---VMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSA-------REYYLRAVCNGFSFR-SYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQSTEALIRSE 537
D GA A++ + YD D + +GL+D ++ +A+G + +S L +DLK +A ++ K +T IKF++ ++ + ++ + ++E ++ L+K+NN+++T K V Y+VD LE+KK QPK ++ + D+ + ++ E L+ N F+ S + P +C LH DPYL+LGPFK+E+ S P+++V +L T ++ ++L+ +TP L+R+R + + GK R IV K+VQ W+ + Y E D+ + N+T+ + LS ++E A LN+T ++SS YQVTNYGL GLCE+H+D E + P L R GD ATIM W+ D P GG T+F P + V PTKG+AA+W+DL + R+ T HGGCP+ GSKWI+NKW++ ++Q + R E
Sbjct: 129 DMYGAAKALIFIVHAYDLDTEKFRKGLVDTTHVKHYAHGAKHQGDSA--LTSQDLKVLATMSQKLGLINTGIKFMRSAYKAQEESEDTEQKDDVIKDQFEKAKQDLIKLNNDFVTNKKTFINDLVVVNKYLVDEKLEKKKTQPKFIKSGAIDKVDISLATSFGGDFFRMEALLQGCRNEFNLLPSKIKWPLISKCRLLHQDDPYLKLGPFKMEVASNTPFIMVVHELLTDEDTEYLVNWATPKLSRSRNLDYSKGAYDKDSFKGKVRNIV-KSVQAWMEAVSYH----EPGDMYNPDNFTVNHPRVHKLSARMELAFNLNLTRQWSSNLYQVTNYGLGGLCESHLDAQSFFENLSSNPETLVNR--GDYVATIMAWLTDTPAGGGTSFFLPPSSLTVHPTKGSAAFWWDLTHQNLRDEGTKHGGCPVAVGSKWILNKWVYSYNQWDKVPCRLE 572 HSP 2 Score: 256.529 bits (654), Expect = 3.501e-69 Identity = 170/519 (32.76%), Postives = 258/519 (49.71%), Query Frame = 0
Query: 1062 NEINLIQALKHFHRAAAETPQNNEGQISQVSQLQHLARLKFELTEKLKPLEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLI----PQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAE------KDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGE--LVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKC 1568
N NL++ F A + T + + + S +++ L + F+ TE LK + E D GA L+ + Y+L + F +G V T + + Q L QD+ LA + + + + FMR + + E KDQF K + + +L N ++T ++ V YLVD+ LEK K QPKF++ K +I+ + R EA + CR E L+ K+K C LH +PYL++ PFKMEV PF M++H+ +++ + +L+ ++ P+LSRSR S K++ + K +R I K+ Q W+ + YH + D P+ N+T +P + LS ++E A + L ++S+ YQVTNYGL GLCE H+D + E + ++ L GD AT MAWL D AGGGT F P V PT+GSAAFW DL R+ + H GCP+ GSKWILNKW+Y ++Q+ C
Sbjct: 78 NSFNLLKKAAVFIPALSNTLSSLKTENS--TEMLKLLEILFQETEVLKTIT------EKDMYGAAKALIFIVHAYDLDTEKFRKGLVDTTHVKHYAHGAKHQGDSALTSQDLKVLATMSQKLGLINTGIKFMRSAYKAQEESEDTEQKDDVIKDQFEKAKQDLIKL-------NNDFVTNKKTFINDLVVVNKYLVDEKLEKKKTQPKFIKSGAIDKVDISLATSFGGDFFRMEALLQGCRNEFNLLPSKIKWPLISKCRLLHQDDPYLKLGPFKMEVASNTPFIMVVHELLTDEDTEYLVNWATPKLSRSRNLDYSKGAYDKDSFKGK-VRNIVKSVQAWMEAVSYH-----EPGDMYNPD----NFTVNHPRVHKLSARMELAFNLNLTRQWSSNLYQVTNYGLGGLCESHLDAQSFFENLS--SNPETLVNRGDYVATIMAWLTDTPAGGGTSFFLPPSSLTVHPTKGSAAFWWDLTHQNLRDEGTKHGGCPVAVGSKWILNKWVYSYNQWDKVPC 569 HSP 3 Score: 232.646 bits (592), Expect = 4.075e-61 Identity = 164/516 (31.78%), Postives = 265/516 (51.36%), Query Frame = 0
Query: 527 DQSTEALIRSEIQTVKSMRELQNNLAQLSQALEEKELVTPPVKEAITVKKV--QISNLMATFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQ---PMGADDTLTLAFEAFKIGWYDHAANLTRFTREHLSLASFSPHQ----RIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDGNLKKKSSQPKFLKRE--DFFNRDPPEVLNQEYEKKEDVMRRICRGE--IVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFS---PTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTL 1026
++S E +R + + +++ + LS L L T E + + ++ Q + ++ T E+ + AK L+ ++ Y + + K D H ++ HQ + + D LA + K+G + R + + + + + E+ +DL++ +N+ + KKT + + + YLVD L+KK +QPKF+K D + ++ + E +++ CR E ++ +++K + +C LH ++P+L+L PFKMEV +PF M++HE+L +++ ++L++++TP LSR R + S K D GK R I+ K+VQ W+E V ++ EP GD Y F+ P +HKLS ++E A N+ LT +WSS YQVTNYGLGGLCESH+D +E P + L + GD +AT MAWL D AGG T+F TV PT+GSAAFW +L R+ + HGGCPV GSK L
Sbjct: 66 NKSVEEYVRHPLNSFNLLKKAAVFIPALSNTLSS--LKTENSTEMLKLLEILFQETEVLKTITEKD-MYGAAKALIFIVHAYDLDTEKFRKGLVDTTH-VKHYAHGAKHQGDSALTSQDLKVLATMSQKLGLINTGIKFMRSAYKAQEESEDTEQKDDVIKDQFEKAKQDLIKLNNDFVTNKKTFINDLVVVNKYLVDEKLEKKKTQPKFIKSGAIDKVDISLATSFGGDFFRMEALLQG-CRNEFNLLPSKIKWPL-ISKCRLLHQDDPYLKLGPFKMEVASNTPFIMVVHELLTDEDTEYLVNWATPKLSRSRNLDYSKGAYDK-DSFKGKVRNIV-KSVQAWMEAVSYH---------------EP--GDMYNPDNFTVNHPRVHKLSARMELAFNLNLTRQWSSNLYQVTNYGLGGLCESHLDAQSFFENLSSNPET--LVNRGDYVATIMAWLTDTPAGGGTSFFLPPSSLTVHPTKGSAAFWWDLTHQNLRDEGTKHGGCPVAVGSKWIL 554
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Match: gi|1325318898|ref|XP_023341213.1| (prolyl 4-hydroxylase subunit alpha-2-like [Eurytemora affinis]) HSP 1 Score: 258.84 bits (660), Expect = 3.399e-71 Identity = 164/499 (32.87%), Postives = 263/499 (52.71%), Query Frame = 0
Query: 60 LRLKALEHKSTTLIELESQALTARKNSLNETTAIFPDRIDYD--GAVYAMVVLKDTYDFDVFQAAQGLID---IEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVI--ATLPEAQRP-VMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSA-------REYYLRAVCNGFSFRSYFEPP--PRF-RCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYN---YTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQSTEALIRSE 537
+ L L H +T+ ES A+ ++ + T + + D GA A++ + Y+ D + G +D I +A+G + +S +L + DL MA + K +T +K ++ F+ + +T P P + E +R L+++NN +IT K V Y+V+ +LE+KK QPK ++ V D + +A E + N F+ FE P P +C LHH DPYL+LGPFK+E+ S P+++V ++ T ++ ++ + +TP L+R+R + + G+ + KK R+V K+VQ W+ ++ Y D+ YN +T+ + +S ++E A LN+T+++SS YQVTNYGL GLCE H D EG+ + + G GD ATIM W+ P GG T+F V V PTKG+AA+W+D+ + +R+R T HGGCP+ GSKWI+NKW++ F+Q R E
Sbjct: 1 MMLNTLTHLNTS----ESTAMKTELVNIIQHTEVLKTVSEKDMYGAAKALIFIIHAYNLDTEKFRVGEVDTTGIRYYAHGAKHQGDS--ELIFLDLVFMAMESKKLGLINTGLKLMRSAFKALENSTEPLTLSPDRQADVEKTKRDLIQLNNNFITNKKTFINDLIVVNQYLVNESLEKKKTQPKFVKSGAVKKIDPRMATAFGGDFFQMEALFQGCRNEFNLLPKFEVPNWPLVSKCRLLHHDDPYLKLGPFKMEVASETPFIMVIHEILTDEDTEYFVNWATPKLSRSRNLDFQKYGKTDK---EKKFRVVAKSVQAWMPEISYHQP-------DNQYNPNNFTVNHPRVHKVSARMELAFNLNLTNQWSSNVYQVTNYGLGGLCEAHFDSQAFFEGINV--EKEGSVNRGDYIATIMAWLTSTPAGGGTSFFSSFSSVTVNPTKGSAAFWWDITHQNYRDRGTKHGGCPVAVGSKWILNKWVYSFNQWNRIPCRLE 481 HSP 2 Score: 248.44 bits (633), Expect = 1.245e-67 Identity = 165/497 (33.20%), Postives = 253/497 (50.91%), Query Frame = 0
Query: 1100 LKFELTEKLKPLEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLI----PQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNS------ALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLRE-EAERRVCRGELVGQKVKDEGPQ-----SCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPNDEFRGLF 1580
+K EL ++ E E D GA L+ + YNL + F G+V T G+ + Q L D++F+A+++ + + + MR F + LT SP Q +++ K + Q N+++T ++ V YLV+++LEK K QPKFV+ G K+ GD + EA + CR E K E P C LH+ +PYL++ PFKMEV PF M++H+ +++ + + + ++ P+LSRSR ++ K K + + KK R+++K+ Q W+ YH D+Q PN N+T +P + +S ++E A + L +++S+ YQVTNYGL GLCE H D + EG+ V + GD AT MAWL AGGGT F S V PT+GSAAFW D+ +R+ + H GCP+ GSKWILNKW+Y F+Q++ C +E + L+
Sbjct: 17 MKTELVNIIQHTEVLKTVSEKDMYGAAKALIFIIHAYNLDTEKFRVGEVDTTGIRYYAHGAKHQGDSELIFLDLVFMAMESKKLGLINTGLKLMRSAFKALENSTEPLTLSPDR-------QADVEKTKRDLIQLNNNFITNKKTFINDLIVVNQYLVNESLEKKKTQPKFVKS-GAVKKIDPRMATAFGGDFFQMEALFQGCRNEF-NLLPKFEVPNWPLVSKCRLLHHDDPYLKLGPFKMEVASETPFIMVIHEILTDEDTEYFVNWATPKLSRSRN-LDFQKYGKTD--KEKKFRVVAKSVQAWMPEISYH-----QPDNQYNPN----NFTVNHPRVHKVSARMELAFNLNLTNQWSSNVYQVTNYGLGGLCEAHFDSQAFFEGINV--EKEGSVNRGDYIATIMAWLTSTPAGGGTSFFSSFSSVTVNPTKGSAAFWWDITHQNYRDRGTKHGGCPVAVGSKWILNKWVYSFNQWNRIPCRLEKKNEDQSLY 490 HSP 3 Score: 234.572 bits (597), Expect = 6.068e-63 Identity = 149/466 (31.97%), Postives = 245/466 (52.58%), Query Frame = 0
Query: 574 VKKVQISNLMATFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQ---PMGADDTLTLAFEAFKIGWYDHAANLTRFTREHLSLAS----FSPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDGNLKKKSSQPKFLKREDFFNRDP--PEVLNQEYEKKEDVMRRICRGE---IVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRR-RIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTL 1026
V +Q + ++ T E+ + AK L+ ++ Y + + + D +R HQ + D + +A E+ K+G + L R + L ++ SP ++ +E+ +DL++ +N + KKT + + + YLV+ +L+KK +QPKF+K DP ++ + E + + CR E + EV N +C LHH++P+L+L PFKMEV ++PF M++HEIL +++ ++ ++++TP LSR R NL K+ + +++ R++ K+VQ W+ ++ ++ + Q +P + P +HK+S ++E A N+ LT +WSS YQVTNYGLGGLCE+H D +EG + + GD +AT MAWL AGG T+F S TV PT+GSAAFW ++ R+ + HGGCPV GSK L
Sbjct: 22 VNIIQHTEVLKTV-SEKDMYGAAKALIFIIHAYNLDTEKFRVGEVD-TTGIRYYAHGAKHQGDSELIFLDLVFMAMESKKLGLINTGLKLMRSAFKALENSTEPLTLSPDRQADVEKTKRDLIQLNNNFITNKKTFINDLIVVNQYLVNESLEKKKTQPKFVKSGAVKKIDPRMATAFGGDFFQMEALFQG-CRNEFNLLPKFEVPNWPLVSKCRLLHHDDPYLKLGPFKMEVASETPFIMVIHEILTDEDTEYFVNWATPKLSRSR-----NLDFQKYGKTDKEKKFRVVAKSVQAWMPEISYHQPDN--------------QYNPNNFTVNHPRVHKVSARMELAFNLNLTNQWSSNVYQVTNYGLGGLCEAHFDSQAFFEGINVEKEGSV--NRGDYIATIMAWLTSTPAGGGTSFFSSFSSVTVNPTKGSAAFWWDITHQNYRDRGTKHGGCPVAVGSKWIL 463
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Match: gi|1108474397|emb|CRK98253.1| (CLUMA_CG011615, isoform A [Clunio marinus]) HSP 1 Score: 233.802 bits (595), Expect = 4.773e-58 Identity = 308/1368 (22.51%), Postives = 546/1368 (39.91%), Query Frame = 0
Query: 257 YLRAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQSTEALIRSEIQTV--------KSMRELQNNLAQLSQALEEKELVTPPVKEAITVKKVQISNLMATFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQPMGADDTLTLAFEAFKIGWYDHAANLTRFTREHLSLASFSPHQRIHLERFTKD---LVRTHNELLRRKKTIV------------GEGFKLLPYLVDGNLKKKSSQPKFLKRED-------FFNRDPPEVLNQ-----------EYEKKEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQAT--NMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWIA--WLGLAMGGKYEFGLFENFVVTEEVFKNEINLIQALKHFHRAAAETPQNNEGQISQVSQLQHLARLKFELTEKLKP-LEKDFGTLEV---DFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIP-QEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRR--------------APFGQDYRVLPYLVDDNLE---KLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGELVGQKVKDEGPQSCYHLH--NQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNK 1555
Y+ A C G + R+ E + RC Y+ + P+ ++ PFKVE S +PY+VVF D+ + E++ + L R + +I + + K HW +D + V +S+++ T LN+ ++ +Q NYG+ G H H + P LG +G+ AT + ++ D GGAT ++P+ + V P KG+A +WY+L G T H CP+L GSKW+ NKWI ++Q E E++ K+ + + L ++ + + + + P+ + +K++ L+ T + + + D + + +T +F +RG VRL N + +D + K + D + + L+ H+ L + D L T N++ + +V G +L+ + + + + + +LK E N P +V+ K+ +++ +C G ++ C ++ +PF ++APFK+E P+ ++ +++++ DEI+ + L R ++P + + +D V+ ++ W+ + G ++SK++ T NM E W Q NYG+GG +H D P + F TG+ +AT + +L+DV+ GG T F V P +GSA FW NL+T E + H CPVL GSK W+A W+ +YE E N+ + L R + + N I + +L L EL + + K+ + + +F+GA+ GL+ L+ Y L +D + NG+I ++++ L+ D+L N K + ++ + N ALT K+Q K ++ L V+++Q ++Y + AP +DY L + NLE L +PK V + T+P G E E + G + + S H +++P+ +IAPFK+E P+ ++ +D +S+ E+ +K RL + + V++ D D + + D + V R I+R M L+ SA ++V NYGL G HI + +++ EV F +G+ +T + +L DV+ GG T F P V P +GS+ FW ++ G + + H CP++ GSKW+ K
Sbjct: 801 YIIAACTGTAKRTDKELS-QVRCRYVS-KSPFSKIAPFKVEEASLDPYIVVFYDVISNDEIEIFKSTAKEGLKRASVLNPDSTSRIDDVRVAK---------LHWQAD--------------------TQGGVFERVSKRVGDMTGLNMK---TAEIWQTQNYGIGG----HYGAHYDFTTPDAAPFHLG---TGNRIATTLFYLSDVEKGGAT--IFPYLRIKVDPRKGSALFWYNLSTSGDVEYYTRHAACPVLVGSKWVANKWIHEYEQ--EWTRPCELKPAPTDANYYKKNQEKAKEELTEMRR--DSYDYILNPLNAILLIKRLSFDFLL-----------TVELIKSIADQFAKNAENITLPYSEFEEAMRGLVRLQNIYKLNPEDLAKGIIDGKK--YRDELS-----ANDLLAFGEVMNHKEFDLPSISLDYLNLALTKNQMTKEVSDLVILEQLYSNYKSIGNTTELVRII--NRILEIAPERDYLKLERINLELSLLLNDKPKDVVENTSPPPLPPGSFNEAKENELISAVCTG--TAKRTDKELSQVHCRYVSK-SPFSKIAPFKVEEASLDPYIVVFYDVISNDEIEIFKSTAKEGLKRASVLNPDS------------------------------TSRIDDVRVAKLHWQADTQGG----------VFERVSKRVGDMTGLNMKTAEIW-----QTQNYGIGGHYGAHYD-------FTTPDAAPFHLGTGNRIATTLFYLSDVEKGGATIFPY--LRIKVDPRKGSALFWYNLSTSGDVEYYTRHVACPVLVGSK---------WVANKWI-----HEYE---------QEWTRPNQEKAKEELTEMRRDSYDYILNPLNAILLIKRLSFDFLLTVELIKSIADQFAKNAENITLPYSEFEGAMRGLVRLQNIYKLNPED------LANGIIDGKKYRD--ELSANDLLAFGEVMNHKEFDLPSISL--DYLNLALT-----KNQMTK---EVSDL-VILEQLYSNYKSIGNTTELVRIINRILEIAP-ERDYLKLERI---NLELSLLLNDKPKDVVEN-------TSPPPLPPGSFNEAKENELISAVCTGTAKRTDKELSQVHCRYVSKSPFSKIAPFKVEEASLDPYIVMFYDVISDDEIEIFKSTAKERLEQ---------------------------------ASVFNADGSLRVDS--ISKFAVMARMDDSEHEV-FKRLIKRHADMTGLNMKSAEKWKVQNYGLGG---HHIPHYDFVKRGEVVP-----FDNGNRISTMLFYLSDVEKGGSTVF--PYLRIKVDPRKGSSLFWFNMNTSGEEDYFTRHAACPVMIGSKWVATK 1945 HSP 2 Score: 154.836 bits (390), Expect = 8.452e-34 Identity = 203/816 (24.88%), Postives = 326/816 (39.95%), Query Frame = 0
Query: 774 RCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQAT--NMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWIAWLGLAMGGKYEFGLFENFVVTEEVF--KNEINLIQALKHFHRAAAETPQNNEGQISQVSQLQHLARLKFELTEKLKP-LEKDFGTLEV---DFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRR--------------APFGQDYRVLPYLVDDNLEK---LKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGELVGQKVKDEGPQSCYHLH--NQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQ 1562
RC ++ + PF ++APFK+E P+ ++ +++++ DEI+ + L R ++P + RVA +H W D QG + ++SK++ T NM E W Q NYG+GG +H D P + F TG+ +AT + +L+DV+ GG T F V P +GSA FW NL+T E + H CPVL GSK V + WI E T+ + KN+ + L R + + N I + +L L EL + + K+ + + +F+ A+ GL+ L+ Y L +D +G + +G ++++ L+ D+L N K + ++ + N ALT K+Q K ++ L V+++Q ++Y + AP +DY L + NLE L +PK V + T+P G E E + G + + S H +++P+ +IAPFK+E P+ ++ +D +S E+ +K L R+ V + D DD V KL+ DT + R +R M L+ +A +Q NYG+ G H D P + +G+ AT + +L DV+ GG T F P V P +GSA FW +L G E + H CP+L GSKW+ NKWI+ ++Q
Sbjct: 821 RCRYVSKS-PFSKIAPFKVEEASLDPYIVVFYDVISNDEIEIFKSTAKEGLKRASVLNPDSTSRIDDVRVAK-----LH-----WQADT---------------------QGGVF---------ERVSKRVGDMTGLNMKTAEIW-----QTQNYGIGGHYGAHYD-------FTTPDAAPFHLGTGNRIATTLFYLSDVEKGGATIFPY--LRIKVDPRKGSALFWYNLSTSGDVEYYTRHAACPVLVGSKW----VANKWIHEYEQEWTRPCEL----KPAPTDANYYKKNQEKAKEELTEMRRDSYDYILNPLNAILLIKRLSFDFLLTVELIKSIADQFAKNAENITLPYSEFEEAMRGLVRLQNIYKLNPEDLAKG--IIDG---KKYRD--ELSANDLLAFGEVMNHKEFDLPSISL--DYLNLALT-----KNQMTK---EVSDL-VILEQLYSNYKSIGNTTELVRIINRILEIAP-ERDYLKLERI---NLELSLLLNDKPKDVVEN-------TSPPPLPPGSFNEAKENELISAVCTGTAKRTDKELSQVHCRYVSKSPFSKIAPFKVEEASLDPYIVVFYDVISNDEIEIFKSTAKEGLKRAS---------------------------------VLNPDSTSRIDDVRVA--KLHWQADTQGGV--FERVSKRVGDMTGLNMKTAEIWQTQNYGIGGHYGAHYD-------FTTPDAAPFHLGTGNRIATTLFYLSDVEKGGATIF--PYLRIKVDPRKGSALFWYNLSTSGDVEYYTRHVACPVLVGSKWVANKWIHEYEQ 1498 HSP 3 Score: 114.775 bits (286), Expect = 1.321e-21 Identity = 112/441 (25.40%), Postives = 194/441 (43.99%), Query Frame = 0
Query: 99 DYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFE---VIATLPEAQ--RPVMSKYESMRRS--LVKVNNEYIT---KKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSAREYY-LRAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
D++GAV + L+ Y + AQG+I NG+ R E L +DL + + Y ++ +LK E + LPE ++ Y +++ +V + ++ + ++ + G + L ++ A+ E K K P+ N E+ +R C+G RS E + C Y + P+L+L P K+E + +P++ ++ D+ + E+D ++ S P L+R E ++ + R+ + QH D+N KV L++++ + LN+ +S +Q NYG+ G E H D + P GL G+ AT + ++ D GG+T V+P+ + V P KG+A +WY+L G T H CP+L GSKW+ NKWI + Q
Sbjct: 122 DFEGAVQGLARLQVMYSLNPEDLAQGII------NGEKYREE----LTAEDLLALGGELMNIKNYLIALPYLKLAEEKNKITNELPELVLLEAMLYNYNQTKKNEEMVDIIDKILNLDPRRDDLKGQKQR-LELIIKAS-EGKLKDPREQARNT---------GMTEFLIMRETCSGRVNRSA-EVISKLHCRY-DTKTPFLKLAPLKLEEANLDPFIAIYHDVMSDNEVDLFILNSRPTLSRASVFEKDASAKVADI------RVAKLSWQH-----------------DNN------DKVFKRLTERVGDMSGLNMK---TSEAWQTQNYGIGGHYEAHYD----FTTKDSAPFTGGL---GNRIATALFYLSDVERGGST--VFPYLRIKVDPKKGSAVFWYNLSTSGELEYYTRHAACPVLVGSKWVANKWIHEYGQ 498 HSP 4 Score: 113.62 bits (283), Expect = 2.798e-21 Identity = 120/466 (25.75%), Postives = 191/466 (40.99%), Query Frame = 0
Query: 1121 DFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVI---KQSQNH-----------YLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYP 1572
DF+GA+ GL L+ Y+L +D +G + NG +E L +D+L L + + Y A+P+++ EK++ P++ L+ ++ Q++ + L RR + L ++ + KLK + R G E+L +RE RV R V K+ C + + P+L++AP K+E PF I HD MS+ E+ + S+P LSR+ E + K +++ K+ +W + D +D++ R ER M L+ ++ +Q NYG+ G E H D S G+ AT + +L DV+ GG T F P V P +GSA FW +L G E + H CP+L GSKW+ NKWI+ + Q C P
Sbjct: 122 DFEGAVQGLARLQVMYSLNPEDLAQG--IINGEKYRE-----ELTAEDLLALGGELMNIKNYLIALPYLK---------LAEEKNKITNELPELVLLEAMLYNYNQTKKNEEMVDIIDKILNLDPRRDDLKGQKQRLELIIKASEGKLKDPREQARNTGM--------TEFLI--MRETCSGRVNRSAEVISKLH------CRY-DTKTPFLKLAPLKLEEANLDPFIAIYHDVMSDNEVDLFILNSRPTLSRA-SVFEKDASAKVADIRVAKL--------SW----------QHDNNDKV------------------FKRLTERVGDMSGLNMKTSEAWQTQNYGIGGHYEAHYD-------FTTKDSAPFTGGLGNRIATALFYLSDVERGGSTVF--PYLRIKVDPKKGSAVFWYNLSTSGELEYYTRHAACPVLVGSKWVANKWIHEYGQEWMRPCELKP 508 HSP 5 Score: 93.9745 bits (232), Expect = 2.823e-15 Identity = 84/288 (29.17%), Postives = 122/288 (42.36%), Query Frame = 0
Query: 752 VMRRICRGEIV-GAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWIAWLG 1038
+MR C G + AEV +++ C + PFL+LAP K+E PF I H++++++E+ + S P LSR S + +VA R + + Q D +F + V D G+ NM +E W Q NYG+GG E+H D + + P F G+ +AT + +L+DV+ GG T F V P +GSA FW NL+T E + H CPVL GSK V + WI G
Sbjct: 270 IMRETCSGRVNRSAEVISKLH---CRY-DTKTPFLKLAPLKLEEANLDPFIAIYHDVMSDNEVDLFILNSRPTLSR-----ASVFEKDASAKVADIR--VAKLSWQHDNNDKVFKRLTERVGDMSGL-------------------------------NMKTSEAW-----QTQNYGIGGHYEAHYD----FTTKDSAP---FTGGLGNRIATALFYLSDVERGGSTVFPY--LRIKVDPKKGSAVFWYNLSTSGELEYYTRHAACPVLVGSKW----VANKWIHEYG 497 HSP 6 Score: 91.2781 bits (225), Expect = 1.947e-14 Identity = 76/283 (26.86%), Postives = 114/283 (40.28%), Query Frame = 0
Query: 1290 VCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYP 1572
C G ++ E Q +++P+ +IAPFK+E P+ ++ +D +S E+ +K L R+ V + D DD V KL+ DT + R +R M L+ +A +Q NYG+ G H D P + +G+ AT + +L DV+ GG T F P V P +GSA FW +L G E + H CP+L GSKW+ NKWI+ ++Q C P
Sbjct: 805 ACTG--TAKRTDKELSQVRCRYVSKSPFSKIAPFKVEEASLDPYIVVFYDVISNDEIEIFKSTAKEGLKRAS---------------------------------VLNPDSTSRIDDVRVA--KLHWQADTQGGV--FERVSKRVGDMTGLNMKTAEIWQTQNYGIGGHYGAHYD-------FTTPDAAPFHLGTGNRIATTLFYLSDVEKGGATIF--PYLRIKVDPRKGSALFWYNLSTSGDVEYYTRHAACPVLVGSKWVANKWIHEYEQEWTRPCELKP 1039
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Match: gi|1321300399|ref|XP_023292094.1| (prolyl 4-hydroxylase subunit alpha-2-like [Lucilia cuprina]) HSP 1 Score: 196.052 bits (497), Expect = 5.585e-47 Identity = 213/883 (24.12%), Postives = 358/883 (40.54%), Query Frame = 0
Query: 738 PPEVLNQEYEKKEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQ-ATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWIAWLGLAMGGKYEFGLFENFVVTEEVFKN----------------EINLIQALKHFHR----------------------AAAETPQNNEGQISQVSQLQHLAR--------LKFELTEKL-KPLEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCR--------------GELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPH-GYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWI 1557
PP V N E R CRG K C + + FLRLAP KME++ P+ +I H+++++ EI+ L + + PNL R + K R ++ T + S+ W F +++K+I Q +M + S QV NYGLGG + H D + + + + GD ++T + +L+DV+ GG T F + + V P +GSA W NL + +A++ H CPV+ GSK V + WI ++T E+ N E+ ++ ++ F R A A P E +++ + L + ++++ E L K +EK E +F+GAI GLL L++TY L D +G + G+ + H DI A+ N R A +++E N T S + ++++L + + Y + Q Y L D+ E++ K +V +G K + ++ D + E V R +L+ + +E C ++ NP+LR+AP K+E + P ++ HD +S+ E+A +++ R+ R+ K+ S + + ++ + I +P+L + R+IE T + + ++S D+QV NYG+ G H PH Y + + + + G+ AT + +L DV+ GGGT F P +Q + P +GSAAFW +L G R+ R+ H CPI+ GSKW+LN+WI
Sbjct: 274 PPVVYNYE---------RGCRGHFSNVTSK-----MYCIYNTTASAFLRLAPIKMEIIHLEPYMVIFHDVISDQEIEHLKNLARPNLKRATVYNDE------------KARSLVVNT-----------------RTSKFSW--------------FQDNTDEITKRINQRIIDMTGFDIVGSEMLQVMNYGLGGHYDKHFDFFNVTQDTPV------VAKEGDRISTVLFYLSDVEQGGATVFPN--IQTAVFPRKGSAVMWYNLNNKVEGDAMTLHAACPVIVGSKW----VCNKWIRSKAQIFRRPCYIYTRVFLIITNEILINAEYYSSVHKMSALMNAELQILNNMETFIRKNENKLEFLKERLKDYEREHEKAVATVPVFFENPVNKYILTKRLTKDWEKIENLMQYQEGEDLLKKIEK-HSVKENEFEGAIDGLLRLQDTYRLNTTDIAQG--ILKGISYNVSFNSRH--CYDIGQRALATNLTRL---AHSWLKEALNKISTDS----------EIKLEKLDIELAVINAKYKLGDISGANQTYVDLINFYPDS-ERIAKL--YVEFLGKSKNKTS-----IKVDYSIDHEPPVIRFKKVNKSILHKYTCADLIRKTPSEERQLRCRYMTETNPFLRLAPIKLEEMNHDPLLVVFHDVISDQEIA-IVKNLTTRIRRA--KIFSGNKSTVSEVRTSQFTFIK---------------------------------VHKHPVLSVIDRRIEDMTNLNM--KYSE-DHQVANYGIGG----HYGPHRDYFSLLHMYPEKLSQYHMGNRIATVLFYLTDVEQGGGTAF--PYMKQHLLPKKGSAAFWYNLHASGVRDKRTLHGACPIVVGSKWVLNRWI 1016 HSP 2 Score: 191.815 bits (486), Expect = 1.110e-45 Identity = 200/839 (23.84%), Postives = 341/839 (40.64%), Query Frame = 0
Query: 276 RFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWI------------------------------FYFD-QSTEALIRSEIQTVKSM----RELQNNLAQLSQALE------EKELVT------PPVKEAITVKKV-----QISNLMATFPEEQTIVDTAK-------------GLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQPMGADDTLTLAFEA---FKIGWYDHAANLTRFTREHL--SLASFSPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGF-KLLPYLVDGN--------LKKKSSQPKFLKREDFFNRDPPEVLNQEYEKKEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPH-GIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWI 1034
+ C Y +LRL P K+E++ PY+V+F D+ + +E++HL + P+L R Y K R +V T S ++D E+ ++Q++ T +I S QV NYGL G + H D + + + K GD +T++ ++ D GGAT V+P+ + V+P KG+A WY+L+ K + T+H CP++ GSKW+ NKWI +Y AL+ +E+Q + +M R+ +N L L + L+ EK + T PV + I K++ +I NLM E + K GLL + DTYR+ + + G ++ +++ ++F + + IG A NLTR L +L S I LE+ +L + + + + + L+ + D KS +K + + +PP + ++ K ++ + +++ + ++ R RC ++ NPFLRLAP K+E + P ++ H+++++ EI + + +T R I N R + +HK P + + ++IE TN+ + S +QV NYG+GG H PH + + P + G+ +AT + +L DV+ GG TAF +Q ++P +GSAAFW NL R+ + HG CP++ GSK V++ WI
Sbjct: 294 KMYCIYNTTASAFLRLAPIKMEIIHLEPYMVIFHDVISDQEIEHLKNLARPNLKRATV-----------YNDEKARSLVVNTRTSKFS--WFQDNTDEITK---------------RINQRIIDMTGFDIV---GSEMLQVMNYGLGGHYDKHFDFFNVTQDTPV------VAKEGDRISTVLFYLSDVEQGGAT--VFPNIQTAVFPRKGSAVMWYNLNNKVEGDAMTLHAACPVIVGSKWVCNKWIRSKAQIFRRPCYIYTRVFLIITNEILINAEYYSSVHKMSALMNAELQILNNMETFIRKNENKLEFLKERLKDYEREHEKAVATVPVFFENPVNKYILTKRLTKDWEKIENLMQYQEGEDLLKKIEKHSVKENEFEGAIDGLLRLQDTYRLNTTDIAQ----------GILKGISYN---------VSFNSRHCYDIGQRALATNLTRLAHSWLKEALNKISTDSEIKLEKLDIELAVINAKYKLGDISGANQTYVDLINFYPDSERIAKLYVEFLGKSKNKTSIKVDYSIDHEPPVIRFKKVNK--SILHKYTCADLI-RKTPSEERQLRCRYMTETNPFLRLAPIKLEEMNHDPLLVVFHDVISDQEIAIVKNLTTR--IRRAKIFSGNKSTVSEVRTSQFTFIKVHK----------------------------------------HPVLSVIDRRIEDMTNLNMK---YSEDHQVANYGIGG----HYGPHRDYFSLLHMYPEKLSQYHMGNRIATVLFYLTDVEQGGGTAFPY--MKQHLLPKKGSAAFWYNLHASGVRDKRTLHGACPIVVGSKW----VLNRWI 1016 HSP 3 Score: 131.339 bits (329), Expect = 7.423e-27 Identity = 116/449 (25.84%), Postives = 203/449 (45.21%), Query Frame = 0
Query: 99 DYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDY------KDLKKMADV----ALKKMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYESMRRSLVKV--NNEYITKKMI-MAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPPP----RFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGS-LEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQS 529
+++GA+ ++ L+DTY + AQG++ + + S H D +L ++A AL K+ D+ IK K E+ + + +S L+ ++E I K + G + VD +++ +P V+R +V +++ + ++ P + RC Y+ +P+LRL P K+E ++ +P LVVF D+ + +E+ ++++ R K N+ + E ++ + I K +H VL + +++E T LN+ KYS +QV NYG+ G H PH + + P +L G+ AT++ ++ D GG TAF Y + +L P KG+AA+WY+L G R++RT+HG CPI+ GSKW++N+WI QS
Sbjct: 635 EFEGAIDGLLRLQDTYRLNTTDIAQGILKGISYN----VSFNSRHCYDIGQRALATNLTRLAHSWLKEALNKISTDSEIKLEKLDIELAVINAKYKLGDISGANQTYVDLINFYPDSERIAKLYVEFLGKSKNKTSIKVDYSIDH---EPPVIRFKKVN--------------KSILHKYTCADLIRKTPSEERQLRCRYMTETNPFLRLAPIKLEEMNHDPLLVVFHDVISDQEI--AIVKNLTTRIRRAKIFSGNKSTVSEVRTSQFTFI--KVHKH---------------------------PVLSVIDRRIEDMTNLNM--KYSE-DHQVANYGIGG----HYGPHRDYFSLLHMYPEKLSQYHMGNRIATVLFYLTDVEQGGGTAFPYMKQHLL--PKKGSAAFWYNLHASGVRDKRTLHGACPIVVGSKWVLNRWIREHGQS 1022 HSP 4 Score: 104.76 bits (260), Expect = 1.036e-18 Identity = 74/284 (26.06%), Postives = 117/284 (41.20%), Query Frame = 0
Query: 1289 RVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQ-TWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTY 1571
R CRG K C + + +LR+AP KME++ P+ +I HD +S+ E+ L ++P L R+ N+ + + + + ++T++ +W D D++ R +R M + QV NYGL G + H D + V A GD +T + +L DV+ GG T F P + V+P +GSA W +L + + H CP++ GSKW+ NKWI Q C Y
Sbjct: 282 RGCRGHFSNVTSK----MYCIYNTTASAFLRLAPIKMEIIHLEPYMVIFHDVISDQEIEHLKNLARPNLKRA---------TVYNDEKARSLVVNTRTSKFSWFQ----------DNTDEIT------------------KRINQRIIDMTGFDIVGSEMLQVMNYGLGGHYDKHFDFFNVTQDTPVVAKE------GDRISTVLFYLSDVEQGGATVF--PNIQTAVFPRKGSAVMWYNLNNKVEGDAMTLHAACPVIVGSKWVCNKWIRSKAQIFRRPCYIY 516
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Match: gi|1108475248|emb|CRK96752.1| (CLUMA_CG010007, isoform A [Clunio marinus]) HSP 1 Score: 193.356 bits (490), Expect = 3.483e-46 Identity = 204/859 (23.75%), Postives = 362/859 (42.14%), Query Frame = 0
Query: 255 EYYLRAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQSTEALI-----RSEIQTVKSMRELQN----------NLAQLSQALEEKEL---------------------VTPPVKEAITVK-------------KVQISNLMATFPEEQTIVD----TAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQPMGADDTLTLAFEAFKIGWYDHAANLTRFTREHLSL------ASFSPHQRIHLERF----------TKDLVRTH---NELLRRKKTIVGE-GFKLLPYLVDGNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEKKEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLE--PFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWIAWLG 1038
E C G + +S E +C Y+ + PY++L FK+E +++PY+V+F D+ T E+ HL+ S + R + SG +R T+ + +++Y +++++ +SQ++E T L++T +S Q+ NYG+ G H PH + E GL G+ AT + + D GG T V+P+ +V + KG+AA+WY+L G + RT H GCP+L GSKW+ NKWI EA++ RS + ++ EL+N +A +++L + ++ +T P+ + VK K +N++ + Q D +GL+ + Y+++ + DF + + ++ P+ D + EA KI + F +E+L L A P + +++E T D + H +ELL R + E ++ + + ++ + D++NR ++E + V ++CRG + + ++ +C ++ + F +LA FK+E P+ ++ H++L++DEI++L + PN+ R +S TLD K S + KL P + D I+++S+++E T + ++ +S QV NYG+GG H PH W+ G + + G+ +AT + +L DV GG T F + V +GSA FW N+ D + + + H CPVL G+K V + WI G
Sbjct: 282 EMIFSQTCRGKNTKSPKEMA-ELKCRYVSNT-PYVKLFRFKLEEANSDPYIVLFLDVVTDNEIFHLIENSKSNFARATTYD----------ASGHRRTSKTRIAK-------------------SSWHYDHQNEIVKKISQRIEHMTGLSMT---TSEGLQIQNYGIGG----HYVPHWDHQIQEAVQYDDGL---GNRVATTLIYFTDVEKGGYT--VFPYLKVRIPAVKGSAAFWYNLKENGQGDYRTRHAGCPVLIGSKWVANKWI------REAVLVSFLSRSNCEIFSAIDELENLAVSEKTILGEIANFTKSLNDSKINKKIAAWQNEYKLVEKDVLAYITNPLNAFLMVKRTTSDVELITKHYKKSSTNVLEKIKDLQPTADDLSGAVEGLMRLQYIYKLKSE-------DFANGIIDGIK--TRSPLAPHDLYVIGDEAAKI------EGSSYFAKEYLELTFKRVKAGLDPLKEVNVEHLLLRLYDVYSGTGDFEKGHSFLDELLARSPGVATEKNIEVAVHRSENRARQSLLTTELDPFSDYYNRSSKWTSDKE----KIVYSQVCRGNL--TKTNAEIAQLKCRYV-SKSAFSKLARFKIEEANLHPYIVVFHDVLSDDEIEFLKVSAKPNVHRAGTLS----------------------------------TTLDVKKTSSRVAKLSWLPDEKD--------EIINRISQRVEDMTGLTMS---TSEMLQVQNYGIGG----HYVPH--WD--HTVKGQKAFSTNGNRIATTLFYLTDVPKGGYTIFIY--LKVRVSAVKGSAVFWYNIRDDGQGDYRTRHAACPVLIGTKW----VANKWIKEYG 1010 HSP 2 Score: 176.407 bits (446), Expect = 7.873e-41 Identity = 243/1097 (22.15%), Postives = 445/1097 (40.57%), Query Frame = 0
Query: 564 VTPPVKEAITVKKVQISNLMAT--FPEE-QTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLR------GRVRLVNHQP-MGADDTLTLAFEAFKIGWYDHAANLTRFTREHLSLA------SFSPHQRIHLERF----------TKDL---VRTHNELLRRKKTIVGEGFKLLPYLVDGNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEKKEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIP-TRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVM--SVWIAWLGLAMGGKYE-FGLFENFVVTEEVFKNEI-NLIQALKHFHRAAAETPQNNEGQISQVSQL------------------------QHLARLKFELTEKLKPLEKDFGTLEVDFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLT------TRRAPFGQDYRVLPYLV--------DDNLE-------KLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPNDEFRGLFE 1581
+T P+ + +K+ + T +PEE + ++ K L D ++ L + +++ L+ G + V +P + D + EA+K+ D+ F E+L LA P + ++++ T D + T +E+++R T+ + F ++ + +L+K + +++ED F D + +K+E + + CRG+ + ++ +C ++ N P+++L FK+E P+ ++ +++ ++EI L++ S N +R S + T R+A H+ + + K+S++IE T + +T +S Q+ NYG+GG H D H I E + G G+ +AT + + DV+ GG T F Y + IP +GSAAFW NL + + + + H GCPVL GSK + +V +++L + + EN V+E+ EI N ++L NE ++ + L +H + + EK+K L+ D GA+ GL+ L+ Y L +DF G + +G+ + +P+ L V +E + + F +E LTF + + +D LK V + H L + F + + L L+ + N+E +Q ++ P+ + N + D + +VCRG L K E Q +++ + ++A FK+E P+ ++ HD +S+ E+ +L +KP + R+ + + +VKK + + K+ +W+ D+ D+++ +R +R M L+ ++ QV NYG+ G H PH ++ +G+ AT + +L DV GG T F + V +GSA FW ++R G + R+ H CP+L G+KW+ NKWI + Q C P EF+ +FE
Sbjct: 78 ITNPLNAFLMIKRTTSDTKLITKKYPEESKDFLENIKDLQPTADDLSGAVEGLLR--LQYIYKLKSVDFANGVIDGVKTRPALSPHDLFVIGDEAYKLKGKDY------FVEEYLVLAYDKVLKGLDPLKEVNVDALLLRMSDVYNRTGDFEFAIHTIDEVIKRNPTV--DIFPVVRRKMVADLEKYGNSI-LIQQEDPFKEDFVKDRTWTNKKEEMIFSQTCRGK--NTKSPKEMAELKCRYVS-NTPYVKLFRFKLEEANSDPYIVLFLDVVTDNEIFHLIENSKSNFARATTYDASGHRRTSKTRIAKSSWHYDHQ----------------------------------------NEIVKKISQRIEHMTGLSMT---TSEGLQIQNYGIGGHYVPHWD-HQIQEAVQYDDG------LGNRVATTLIYFTDVEKGGYTVF---PYLKVRIPAVKGSAAFWYNLKENGQGDYRTRHAGCPVLIGSKWVANKWIREAVLVSFLSRSNCEIFSAIDELENLAVSEKTILGEIANFTKSLNDSKINKKIAAWQNEYKLVEKDVLAYITNPLNAFLMVKRTTSDVELITKHYKKSSTNVLEKIKDLQPTAD----DLSGAVEGLMRLQYIYKLKSEDFANG--IIDGIKTRSPLAPHDL--------YVIGDEAAKIEGSSYFAKEYLE--LTFK--------RVKAGLDPLKEV---NVEHLLLRLYDVYSGTGDFEKGHSFLDELLARSPGVATEKNIEVAVHRSENRARQSLLTTELDPFS-DYYNRSSKWTSDKEKIVYSQVCRGNLT--KTNAEIAQLKCRYVSKSAFSKLARFKIEEANLHPYIVVFHDVLSDDEIEFLKVSAKPNVHRAG-TLSTTLDVKKTSSRVAKL--------SWLP----------DEKDEII------------------NRISQRVEDMTGLTMSTSEMLQVQNYGIGG----HYVPHW----DHTVKGQKAFSTNGNRIATTLFYLTDVPKGGYTIF--IYLKVRVSAVKGSAVFWYNIRDDGQGDYRTRHAACPVLIGTKWVANKWIKEYGQEFRRPC---PPGEFKEVFE 1027 HSP 3 Score: 101.679 bits (252), Expect = 8.384e-18 Identity = 74/270 (27.41%), Postives = 127/270 (47.04%), Query Frame = 0
Query: 261 VCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDPH--GSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
VC G ++ E + +C Y+ + + +L FK+E + +PY+VVF D+ + E++ L + + P++ R G + KK + WL D + +++ +SQ++E T L ++ +S QV NYG+ G H PH +++G + +G+ AT + ++ D P GG T F+Y +V V KG+A +WY++ G + RT H CP+L G+KW+ NKWI + Q
Sbjct: 788 VCRGNLTKTNAEIA-QLKCRYVS-KSAFSKLARFKIEEANLHPYIVVFHDVLSDDEIEFLKVSAKPNVHRA--------GTLSTTLDVKKTSSRVAKLS-WLPDE--------------------KDEIINRISQRVEDMTGLTMS---TSEMLQVQNYGIGG----HYVPHWDHTVKG------QKAFSTNGNRIATTLFYLTDVPKGGYTIFIYL--KVRVSAVKGSAVFWYNIRDDGQGDYRTRHAACPVLIGTKWVANKWIKEYGQ 1011
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Match: gi|1061484083|gb|ODN01274.1| (Prolyl 4-hydroxylase subunit alpha-1 [Orchesella cincta]) HSP 1 Score: 169.474 bits (428), Expect = 2.380e-41 Identity = 132/477 (27.67%), Postives = 210/477 (44.03%), Query Frame = 0
Query: 1121 DFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSA--------LTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKK-------QPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATD-YQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPNDEFRGLFE 1581
DF G L+ L++TY L + + + G + T + + SPY L+ +D LFL A K YD AV ++ + A L A K++ F R+ + + +R P+G+ ++ ++ L K++K QPK + + P + N E + +CRGE + + K + P C +L N + +K+E + P+ I HDFMSE EM + E+S RL RS + + + + I + Q W+ D P++ +S +I AT +++ A++ YQV NYG++G H D Y+ G + + L G+ ATFM +L+DV AGG T F G + V P +G+A FW +LR G + + H GC +L G+KWI NKWI QF C P++E F+
Sbjct: 27 DFIGGSMALVGLQDTYALNLSEIIHGDLSTENAVQK---SPYELSARDCLFLGKAAFNKGLYDRAVGWLEMALSRAKEEYEDETLLIPTATKEEILPFLETTRRVHDEVLE--------KRGPYGETWQTFEKPINGKLAKMRKYQKIKRYQPKIMVPLTPVEEN--------------ENFKALCRGENL-RSPKLDAPLRCRYLRRPNSVSILKAYKVEEMSLDPYIAIYHDFMSEYEMGYFKEHSTSRLKRSLHQGRDGRYIATD---------IRTSKQAWLNK-------------------------DDGPLIDKISARIGDATGLEVFPENEASEIYQVANYGMAGRYSPHTD---YITGDDHLHNPLEL-VRGNRIATFMVYLEDVPAGGSTVFPLIGVQ--VKPRKGAAVFWWNLRTDGRGDPWTRHAGCSVLYGNKWITNKWIRYHHQFRRIPCVKEPDNERTASFQ 437 HSP 2 Score: 135.961 bits (341), Expect = 4.878e-30 Identity = 118/446 (26.46%), Postives = 201/446 (45.07%), Query Frame = 0
Query: 94 FPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVF---------EVIATLPEAQRPVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTV-QHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQ-YQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
P D+ G A+V L+DTY ++ + G + E +SP++L +D + A K YD ++ +L+ E + + ++ E+ RR V++E + K+ G ++ ++ L + +K K+ R + + E + +A+C G + RS P RC YL + L +KVE +S +PY+ ++ D ++ EM + ST L R+ +H+ + G+ +T Q WL+ KD + ++ +S ++ AT L + + +++ YQV NYG+AG H D + G + + L L + G+ AT M +++D P GG+T V+P V V P KGAA +W++L G + T H GC +L G+KWI NKWI Y Q
Sbjct: 22 LPSFEDFIGGSMALVGLQDTYALNLSEIIHGDLSTENAVQ------KSPYELSARDCLFLGKAAFNKGLYDRAVGWLEMALSRAKEEYEDETLLIPTATKEEILPFLETTRR----VHDEVLEKRGPY-GETWQTFEKPINGKLAKMRKYQKIKRYQPKIMVPLTPVEENENF-KALCRGENLRSPKLDAP-LRCRYLRRPNSVSILKAYKVEEMSLDPYIAIYHDFMSEYEMGYFKEHSTSRLKRS----------LHQGRDGRYIATDIRTSKQAWLN-----------KD---------DGPLIDKISARIGDATGLEVFPENEASEIYQVANYGMAGRYSPHTD---YITGDDHLHNPLELVR-GNRIATFMVYLEDVPAGGST--VFPLIGVQVKPRKGAAVFWWNLRTDGRGDPWTRHAGCSVLYGNKWITNKWIRYHHQ 418 HSP 3 Score: 108.227 bits (269), Expect = 9.589e-21 Identity = 119/467 (25.48%), Postives = 189/467 (40.47%), Query Frame = 0
Query: 586 FPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQP--MGADDTLTLAFEAFKIGWYDHAAN-----LTRFTREHLSLASFSP-HQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDGNLKKKSSQPKFLKREDFFNRDPPEVLN--QEYEKKEDVMRRICRGEIVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNM-ILTERWSSTKYQVTNYGLGGLCESHIDPHGIWEGAEIPPGSRFLHST-----GDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSKMTLVAVMSVWIAW 1036
P + + + L+ + DTY + + + +H V P + A D L L AF G YD A L+R E+ P + + F + R H+E+L K+ GE ++ ++G L K +++ R P+++ E+ E+ + +CRGE + + + RC +L N L +K+E + P+ I H+ ++E E+ + ++ST L R +L + R I + Q W+ KD P I K+S +I AT + + E +S YQV NYG+ G H D G LH+ G+ +ATFM +L DV AGG T F G + V P +G+A FW NL TD R + + H GC VL G+K + + WI +
Sbjct: 22 LPSFEDFIGGSMALVGLQDTYALNLS-------EIIHGDLSTENAVQKSPYELSARDCLFLGKAAFNKGLYDRAVGWLEMALSRAKEEYEDETLLIPTATKEEILPFLETTRRVHDEVLE-KRGPYGETWQTFEKPINGKLAK-------MRKYQKIKRYQPKIMVPLTPVEENEN-FKALCRGENLRSPKLDAP--LRCRYLRRPNSVSILKAYKVEEMSLDPYIAIYHDFMSEYEMGYFKEHSTSRLKR-------SLHQGRDGRYIATD---IRTSKQAWLN-----------KDD-------------------GPLIDKISARIGDATGLEVFPENEASEIYQVANYGMAGRYSPHTD---------YITGDDHLHNPLELVRGNRIATFMVYLEDVPAGGSTVFPLIGVQ--VKPRKGAAVFWWNLRTDGRGDPWTRHAGCSVLYGNKW----ITNKWIRY 415
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Match: gi|1228404351|ref|XP_021961276.1| (prolyl 4-hydroxylase subunit alpha-2-like [Folsomia candida] >gi|1215259448|gb|OXA45773.1| Prolyl 4-hydroxylase subunit alpha-2 [Folsomia candida]) HSP 1 Score: 160.614 bits (405), Expect = 1.713e-37 Identity = 136/469 (29.00%), Postives = 216/469 (46.06%), Query Frame = 0
Query: 1121 DFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVV-----IKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLL-SRFSATDYQVTNYGLSGLCEGHIDPHGYLEG---MEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPNDEFRGLF 1580
DF G L+ L++TY L + + + G + + +PQ SPY L+ +D+LFL A K YD ++ ++ AL+ S E + F P R +++ K+ + L +R P G++++ VD +KL KQ K++ + YK + P L G E + +CRG + + K + P C ++ I PF++E P+ + ++DFM+E EM + + RL RS + + V + I + Q W+ Q+D ++ DT +SR+I AT +++ +A +QV NYG++G H D YL G + P RR G+ ATFM +L++V AGG T F G + V P +G+A FW +L+ G + + H GC +L GSKWI NKW+ QF C P D F
Sbjct: 137 DFIGGSMALVGLQDTYGLNLSEVIYGTLGSES-VPQ---SPYELSARDLLFLGKAAFNKGLYDRSISWLE----MALSRSEEESQSVSFFSPTATREEILPFLETTKRVHDDVLD-KRGPTGENWQTFLKPVD---QKLAKQKKYL-SVKKYKPKLYTP---LNGAEENENYKALCRG-IRLRSPKLDAPLRCRYVARPLTRSFIGPFQIEEHAIEPYIVTINDFMNEDEMKSFRDTASSRLKRSLHQGRDGQYVATD---------IRTSKQAWL-----------TQEDG--------SFVDT------ISRRISDATGLEIFPENLAAEIFQVANYGMAGRYTAHTD---YLTGEDHIRNPLEIRR----GNRIATFMVYLENVGAGGSTVFPYIGVQ--VKPKKGTAVFWWNLKRDGSGDPLTRHAGCSVLYGSKWITNKWVRYNAQFQRALCIPEPEDNRSASF 545 HSP 2 Score: 134.035 bits (336), Expect = 9.779e-29 Identity = 126/461 (27.33%), Postives = 202/461 (43.82%), Query Frame = 0
Query: 94 FPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFE--------VIATLPEAQRP-VMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQPKVLRENQVTLADMGVQSAREYYLRAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTV-QHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNI-THKYSSTQYQVTNYGLAGLCETHVDPHGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQSTEALIRSEIQTVKS 543
P D+ G A+V L+DTY ++ + G + E +SP++L +DL + A K YD SI +L+ V P A R ++ E+ +R V+++ + K+ G N++ VD L ++KK V + + E Y +A+C G RS P RC Y+ +GPF++E + PY+V D + EM ++ L R+ +H+ + G+ +T Q WL+ D ++ TI S+++ AT L I ++ +QV NYG+AG H D L G + + L +R+ G+ AT M ++++ GG+T V+P+ V V P KG A +W++L G + T H GC +L GSKWI NKW+ Y Q AL E + +S
Sbjct: 132 MPTFDDFIGGSMALVGLQDTYGLNLSEVIYGTLGSESVP-------QSPYELSARDLLFLGKAAFNKGLYDRSISWLEMALSRSEEESQSVSFFSPTATREEILPFLETTKR----VHDDVLDKRG-PTGENWQTFLKPVDQKLAKQKKYLSVKKYKPKLYTPLNGAEENENY-KALCRGIRLRSPKLDAP-LRCRYVARPLTRSFIGPFQIEEHAIEPYIVTINDFMNEDEMKSFRDTASSRLKRS----------LHQGRDGQYVATDIRTSKQAWLTQE------------DGSFVDTI--------SRRISDATGLEIFPENLAAEIFQVANYGMAGRYTAHTD---YLTGEDHIRNPLEIRR-GNRIATFMVYLENVGAGGST--VFPYIGVQVKPKKGTAVFWWNLKRDGSGDPLTRHAGCSVLYGSKWITNKWVRYNAQFQRALCIPEPEDNRS 542 HSP 3 Score: 97.4413 bits (241), Expect = 7.443e-17 Identity = 112/415 (26.99%), Postives = 174/415 (41.93%), Query Frame = 0
Query: 636 MGADDTLTLAFEAFKIGWYDHAAN-----LTRFTREHLSLASFSP-HQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDGNLKKKSSQPKFLKREDFFNRDPPEV---LNQEYEKKEDVMRRICRGEIVGAEVKNQVRFQ--------RCHWLHHNNPFLR--LAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGKRRRI-IHKTVQCWI--EDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNM-ILTERWSSTKYQVTNYGLGGLCESHID----PHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
+ A D L L AF G YD + + L+R E S++ FSP R + F + R H+++L K+ GE ++ VD L K Q K+L + + P++ LN E + + +CRG +R + RC ++ P R + PF++E P+ + +++ +NEDE++ D ++ S LK + H G+ I + Q W+ ED F + +S++I AT + I E ++ +QV NYG+ G +H D I EI G+R +ATFM +L +V AGG T F G + V P +G+A FW NL D + L+ H GC VL GSK
Sbjct: 176 LSARDLLFLGKAAFNKGLYDRSISWLEMALSRSEEESQSVSFFSPTATREEILPFLETTKRVHDDVLD-KRGPTGENWQTFLKPVDQKLAK---QKKYLSVKKY----KPKLYTPLNGAEENEN--YKALCRG----------IRLRSPKLDAPLRCRYVAR--PLTRSFIGPFQIEEHAIEPYIVTINDFMNEDEMKSFRDTAS-----------SRLKRSLHQGRDGQYVATDIRTSKQAWLTQEDGSF--------------------------------VDTISRRISDATGLEIFPENLAAEIFQVANYGMAGRYTAHTDYLTGEDHIRNPLEIRRGNR--------IATFMVYLENVGAGGSTVFPYIGVQ--VKPKKGTAVFWWNLKRDGSGDPLTRHAGCSVLYGSK 515
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Match: gi|1061483941|gb|ODN01138.1| (Prolyl 4-hydroxylase subunit alpha-2, partial [Orchesella cincta]) HSP 1 Score: 157.147 bits (396), Expect = 1.238e-36 Identity = 126/494 (25.51%), Postives = 206/494 (41.70%), Query Frame = 0
Query: 1121 DFDGAITGLLVLKETYNLPMKDFLRGKVVTNGLIP----QEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMRE---VFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVDDNLEKLKKQPKFVRQMGPYKRNITNPNE---YLQGDLREEAER--RVCRGELVGQKVKDEGPQSCYHLHNQ-------------------------NPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPNDEFR 1577
D +GA L+ L++TY L M +G V+ + + Q FQS L+ +D+LF+ A + Y A+ +++ + N + S + Q I+ V++ R+ P G ++ P ++ L K Y N + Y + EEAE R+CRGE + P C+ + Q +PYL I+P K+E P+ +I+HDF++ ++ +++ KP+L RSR + +H D+ D + + +P++ ++R++ T +K + A DYQ+ NYGL GL H D + + R GD AT M +L DVD GG T F G +WP +G+AAFW +L G + + H CP+L G+KW+ NKWI +QF+ CG +P D+ +
Sbjct: 54 DLNGAAQALVRLQDTYQLDMPSLAQGNVMPSQQLSAENEQAFQSTSQLSARDMLFMGRHALSQGLYHRAIDWLQHAELLANGEMNSSSVLSEISPSLQNAIEAHDDVLE---------RQGPRGVSWKTNPIPINPALANSAK----------YSLNTSTTFHVKLYQRQTEEEEAEHFLRLCRGENIRVNTCKVIPSFCHGIFQQMKRALILFQPPVLTAALKCRLKTDTSPYLLISPLKIEEQSHDPYVVIVHDFITSSQADEIIKLGKPKLFRSRHR--------------------------------HHQDEVMDVVSHKRVSQNAWIKDWEHPLMHKVTRRVYLVTGLKAIQPKEAEDYQIANYGLGGLYVPHTDHLMNNPDKSIYSPWERFV--GDRIATLMIYLSDVDGGGATVFTRAGV--TLWPEKGAAAFWWNLDKSGIGDDNTRHGACPVLHGNKWVSNKWIRFNEQFNTAPCGRHPLDKHK 492 HSP 2 Score: 144.436 bits (363), Expect = 1.937e-32 Identity = 122/469 (26.01%), Postives = 202/469 (43.07%), Query Frame = 0
Query: 94 FPDRIDYDGAVYAMVVLKDTYDFDVFQAAQG-LIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYESMRRSLVKVNNEYITKKMIMAGANYKVLPYVVDANLERKKKQP-KVLRENQVTLADMGVQSAR-EYYLRAVCNGFSFRS----------------------YFEPP---PRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQHWLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDP--HGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQSTEA 532
FP D +GA A+V L+DTY D+ AQG ++ ++ + +S +L +D+ M AL + Y +I +L+ E++A V+S+ ++ ++ +++ + ++ G ++K P ++ L K V L + E++LR +C G + R F+PP +C PYL + P K+E S +PY+V+ D T + D ++ P L R+R ++E V V H K+V ++ + + KV ++++ T L + YQ+ NYGL GL H D + + + P R GD AT+M ++ D GGAT F V +WP KGAAA+W++LD G + T HG CP+L G+KW+ NKWI + +Q A
Sbjct: 49 FPQADDLNGAAQALVRLQDTYQLDMPSLAQGNVMPSQQLSAENEQAFQSTSQLSARDMLFMGRHALSQGLYHRAIDWLQHA-ELLANGEMNSSSVLSEISPSLQNAIEAHDDVLERQG-PRGVSWKTNPIPINPALANSAKYSLNTSTTFHVKLYQRQTEEEEAEHFLR-LCRGENIRVNTCKVIPSFCHGIFQQMKRALILFQPPVLTAALKCRLKTDTSPYLLISPLKIEEQSHDPYVVIVHDFITSSQADEIIKLGKPKLFRSRHRHHQDE--------------VMDVVSH---------KRVSQNAWIKDWEHPLMHKV----TRRVYLVTGLKAIQPKEAEDYQIANYGLGGLYVPHTDHLMNNPDKSIYSPWERF----VGDRIATLMIYLSDVDGGGATVFT--RAGVTLWPEKGAAAFWWNLDKSGIGDDNTRHGACPVLHGNKWVSNKWIRFNEQFNTA 481 HSP 3 Score: 98.2117 bits (243), Expect = 2.560e-17 Identity = 113/487 (23.20%), Postives = 190/487 (39.01%), Query Frame = 0
Query: 585 TFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQP------MGADDTLTLAFEAFKIG-------WYDHAANLTRFTREHLS-LASFSPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVD---GNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEKKEDVMRRICRGEIVGAEV----------------KNQVRFQ--------RCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNL--SRERGISPSNLKLTKHDRVAGKRRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHID-----PHGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
FP+ + A+ L+ + DTY++ + +L + + + P + ++ N Q + A D L + A G W HA L S L+ SP ++ + H+++L R+ G +K P ++ N K S + R Q E++ + R+CRGE + + + FQ +C +P+L ++P K+E P+ +I+H+ + + ++ P L SR R + + H RV+ WI+D W+ P +HK+++++ T + + + YQ+ NYGLGGL H D P + + P RF+ GD +AT M +L+DV GG T F G T+ P +G+AAFW NL + + HG CPVL G+K
Sbjct: 48 NFPQADDLNGAAQALVRLQDTYQLDMPSLAQGN---VMPSQ-QLSAENEQAFQSTSQLSARDMLFMGRHALSQGLYHRAIDWLQHAELLANGEMNSSSVLSEISPS--------LQNAIEAHDDVLERQGP-RGVSWKTNPIPINPALANSAKYSLNTSTTFHVKLYQR-------QTEEEEAEHFLRLCRGENIRVNTCKVIPSFCHGIFQQMKRALILFQPPVLTAALKCRLKTDTSPYLLISPLKIEEQSHDPYVVIVHDFITSSQADEIIKLGKPKLFRSRHRHHQDEVMDVVSHKRVSQN----------AWIKD----------------WE--------------HPLMHKVTRRVYLVTGLKAIQPKEAEDYQIANYGLGGLYVPHTDHLMNNP----DKSIYSPWERFV---GDRIATLMIYLSDVDGGGATVFTRAGV--TLWPEKGAAAFWWNLDKSGIGDDNTRHGACPVLHGNK 465
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Match: gi|321461762|gb|EFX72791.1| (hypothetical protein DAPPUDRAFT_308081 [Daphnia pulex]) HSP 1 Score: 157.532 bits (397), Expect = 2.370e-36 Identity = 134/469 (28.57%), Postives = 211/469 (44.99%), Query Frame = 0
Query: 75 LESQALTARKNSLNET-----TAIFPDRIDYDGAVYAMVVLKDTYDFDVFQAAQGLIDIEKFANGQPLRMESPHKLDYKDLKKMADVALKKMWYDTSIKFLKGVFEVIATLPEAQRPVMSKYESMRRSLVKVNNEY---ITKKMIMAGANYKVLPYVVDANLERKKKQP-KVLRENQVTLADMGVQSA---REYYLRAVCNGFSFRSYFEPPPRFRCAYLHHQDPYLRLGPFKVEMVSTNPYLVVFQDLFTQKEMDHLLIESTPHLTRTRKPELKNEGQIHEYKSGKKRRIVTKTVQH-WLSDLVYEDKKVEVKDIDDNYNYTIESKVLFGLSQKLERATRLNITHKYSSTQYQVTNYGLAGLCETHVDP--HGSLEGVELPPSRLGLRKSGDMFATIMGWIQDEPVGGATAFVYPHKEVLVWPTKGAAAYWYDLDLKGFRNRRTVHGGCPILKGSKWIVNKWIFYFDQ 528
LE+++ ++R N+LN T++FP+ D GA A+V L+DTY +V A G + + GQ +S L +D MA A +YDT++++ + A + + + + + V+ + + ++ G N++ + VD + L E Q T QS E+Y R +C G RS RC + P L L P KVE S +P +VV DL T+++ + L P L + +H GK R + +T ++ WL + E+ L + ++E AT L + +S +Q+ NYG+ GL +TH D H + + P L + GD AT+M ++ D GGAT V+P V WP KG+AA+W++L G + T HG CP+L GSKW+ NKWI +DQ
Sbjct: 114 LEAESNSSR-NALNHVLVLRETSVFPNSEDLHGAAIALVRLQDTYQLNVTDLADGHYNGVDHSKGQVF--QSRRDLSGRDCLFMAQHAFADGYYDTALQWAEAALGQFARKGDNYGSELETFRNEVKHFVEFASRVHDDVLERQGPRGVNWQTNSHPVDRQFRSGTSEKYDSLAEQQFTPKLYQHQSEDEENEHYER-LCRGEKLRSA-NIEAGLRCRLVTRGHPALLLQPIKVEEQSLDPMIVVLHDLITERQTEILRQLGEPKLATS----------LHRGGEGKFVRSMIRTSKNAWLQEH--------------------ENASLPAIRHRMELATGLIYGPETASEYFQIANYGIGGLYKTHTDNVIHPDVRPEDQDPWNLYV---GDRIATLMVYLSDVEAGGAT--VFPRAGVTCWPRKGSAAFWWNLYKSGEPDLTTRHGACPVLHGSKWVSNKWIRQYDQ 542 HSP 2 Score: 147.132 bits (370), Expect = 5.951e-33 Identity = 121/474 (25.53%), Postives = 196/474 (41.35%), Query Frame = 0
Query: 1121 DFDGAITGLLVLKETYNLPMKDFLRGKVVTNGL---IPQEFQSPYHLNVQDILFLAVKANEKRWYDAAVPFMREVFNSALTFSPAEKDQFAKFQPQIDRLKVVIKQSQNHYLTTRRAPFGQDYRVLPYLVD-----------DNLEKLKKQPKFVRQMGPYKRNITNPNEYLQGDLREEAERRVCRGELVGQKVKDEGPQSCYHLHNQNPYLRIAPFKMEVLLRRPFRMILHDFMSEAEMAWLLEYSKPRLSRSREKVESNKEVKKNNLQNKKIRIISKTTQTWIGSRVYHGDDEFDQDDQLVPNPKLYNYTDTNPMLVSLSRKIERATKMKLLSRFSATDYQVTNYGLSGLCEGHIDPHGYLEGMEVPASRRRLFASGDIFATFMAWLDDVDAGGGTGFESPGFEQVVWPTRGSAAFWIDLRPLGHRELRSSHTGCPILKGSKWILNKWIYLFDQFHHYKCGTYPNDEFRGLF 1580
D GA L+ L++TY L + D G NG+ Q FQS L+ +D LF+A A +YD A+ + + F+ ++ + + L R+ P G +++ + VD D+L + + PK + + N E R+CRGE + + E C + +P L + P K+E P ++LHD ++E + L + +P+L+ S + K V+ +I + W+ N L ++ ++E AT + ++ +Q+ NYG+ GL + H D + + L+ GD AT M +L DV+AGG T F G WP +GSAAFW +L G +L + H CP+L GSKW+ NKWI +DQ KCG +P +++ L
Sbjct: 142 DLHGAAIALVRLQDTYQLNVTDLADGHY--NGVDHSKGQVFQSRRDLSGRDCLFMAQHAFADGYYDTALQWAEAALGQFARKGDNYGSELETFRNEVKHFVEFASRVHDDVLE-RQGPRGVNWQTNSHPVDRQFRSGTSEKYDSLAEQQFTPKLYQHQSEDEEN--------------EHYERLCRGEKL-RSANIEAGLRCRLVTRGHPALLLQPIKVEEQSLDPMIVVLHDLITERQTEILRQLGEPKLATSLHRGGEGKFVRS---------MIRTSKNAWLQEH-------------------------ENASLPAIRHRMELATGLIYGPETASEYFQIANYGIGGLYKTHTDNVIHPDVRPEDQDPWNLYV-GDRIATLMVYLSDVEAGGATVFPRAGV--TCWPRKGSAAFWWNLYKSGEPDLTTRHGACPVLHGSKWVSNKWIRQYDQMFVAKCGLHPLEKYDSLL 560 HSP 3 Score: 132.494 bits (332), Expect = 3.762e-28 Identity = 118/449 (26.28%), Postives = 202/449 (44.99%), Query Frame = 0
Query: 584 ATFPEEQTIVDTAKGLLMVMDTYRIQIDALTKRDWDFLHPLRGRVRLVNHQPMGADDTLTLAFEAFKIGWYDHA-----ANLTRFTREHLSLASFSPHQRIHLERFTKDLVRTHNELLRRKKTIVGEGFKLLPYLVDGNLKKKSSQPKFLKREDFFNRDPPEVLNQEYEKKEDVMRRICRGE-IVGAEVKNQVRFQRCHWLHHNNPFLRLAPFKMEVLLKSPFRMILHEILNEDEIQWLMDYSTPNLSRERGISPSNLKLTKHDRVAGK-RRRIIHKTVQCWIEDVMFNATLDAVKDSEGIWKLEPFQGDPYASRIFSPTIHKLSKKIEQATNMILTERWSSTKYQVTNYGLGGLCESHIDP--HGIWEGAEIPPGSRFLHSTGDMLATFMAWLNDVKAGGLTAFDSEGYEQTVIPTRGSAAFWINLATDLRREALSSHGGCPVLAGSK 1023
+ FP + + A L+ + DTY++ + L ++ + +G+V + + + D L +A AF G+YD A A L +F R+ + S R ++ F + R H+++L R+ G ++ + VD + +S+ E F P +Q +++ + R+CRGE + A ++ +R C + +P L L P K+E P ++LH+++ E + + L P L+ + H GK R +I + W+++ NA+L A++ ++E AT +I +S +Q+ NYG+GGL ++H D H + P + ++ GD +AT M +L+DV+AGG T F G T P +GSAAFW NL + + HG CPVL GSK
Sbjct: 135 SVFPNSEDLHGAAIALVRLQDTYQLNVTDLADGHYNGVDHSKGQV-FQSRRDLSGRDCLFMAQHAFADGYYDTALQWAEAALGQFARKGDNYGSELETFRNEVKHFVEFASRVHDDVLERQGP-RGVNWQTNSHPVDRQFRSGTSEKYDSLAEQQFT--PKLYQHQSEDEENEHYERLCRGEKLRSANIEAGLR---CRLVTRGHPALLLQPIKVEEQSLDPMIVVLHDLITERQTEILRQLGEPKLA-----------TSLHRGGEGKFVRSMIRTSKNAWLQE-HENASLPAIR-----------------------------HRMELATGLIYGPETASEYFQIANYGIGGLYKTHTDNVIHPDVRPEDQDPWNLYV---GDRIATLMVYLSDVEAGGATVFPRAGV--TCWPRKGSAAFWWNLYKSGEPDLTTRHGACPVLHGSK 530 The following BLAST results are available for this feature:
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 10
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 17
Pagesback to top
BLAST of prolyl 4-hydroxylase subunit alpha-1 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold359_size197282:60130..65542+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold359_size197282-processed-gene-0.4 ID=snap_masked-scaffold359_size197282-processed-gene-0.4|Name=prolyl 4-hydroxylase subunit alpha-1|organism=Tigriopus kingsejongensis|type=gene|length=5413bp|location=Sequence derived from alignment at scaffold359_size197282:60130..65542+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'prolyl 4-hydroxylase subunit alpha-1' has the following synonyms
Add to Basket
|