4-coumarate-- ligase-like, snap_masked-scaffold444_size168727-processed-gene-0.25 (gene) Tigriopus kingsejongensis

Overview
Name4-coumarate-- ligase-like
Unique Namesnap_masked-scaffold444_size168727-processed-gene-0.25
Typegene
OrganismTigriopus kingsejongensis (Tigriopus kingsejongensis)
Associated RNAi Experiments

Nothing found

Homology
BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 204.912 bits (520), Expect = 1.166e-58
Identity = 162/560 (28.93%), Postives = 268/560 (47.86%), Query Frame = 0
Query:   73 SVIGSNNILTSHDPLNNADNPVTAPDKHMVEVYFEAIQARIEKVGNARYCTDG----LTGEFNRIG-------DLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPD--LLTTCMF-HISGIGKLLMGPLNQEAI---FFKDSDLLK-DITLILKACHDYKCGKVFLGSHHCAQLANL---SEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNM-NFLNCYSMTELGFLVANSMDPHCLGSLAEGAQVKIQDPVTQTALGPLQTGEILVK-PPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDL-PEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRN 608
            S I  N ILTS +  +  D+ V   ++++   + ++++  + K  N  +  +       GE    G       D   +  + AK     G+++GD+V I I N  +    V  T +C  + S++  ++S  TL ++V++T+AK +ICT+          K     + I+ LD   H   + +  +ST +Q+         PE + +F L   A I W+SGTTG PKGI    QL    +   L   P  D  +L TC F H+SG        +N +     F    D+    +  I+          + + + H   +A      +I    +   V  + P GA     +   L  +F N+ N  N Y+MTE G L+  S D    G +    +V I+D  +   LGP  +GE++ + P  P  GY N+P+ET KF   DG+C +GD  HY++ G L+ +GR+KE++K   +HVYP+E+E +IQ +   + E G++ K +   Q +   +V +  G ++  E I++ V + +D+YK L   +  TN PLP+N  GK +RN
Sbjct:    7 SKINENKILTSQNDFD--DSVVLEYNQNITSYFIDSMKKYMTKNSNKEFLVNCGKKYFPGETTSEGPESVSILDFIEKVPKFAKYLYENGLRKGDVVHIIIGNRGDVYIPVHATLLCQGVVSISVDNISTETLLSEVQETQAKFVICTNRTY------YKVKDLSSIIINLDTQLHA--ILEIDMSTQTQI---------PEQI-EFDLQTLAVILWTSGTTGKPKGI----QLNMVSLWNLLFLSPKTDGNMLITCNFAHVSGFNYSCFWSINFDVCTFHFISPEDVTSLGVEKIIFTLSQIPFTTIVVSASHIVYIAEKIVNDQIEYSSNFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVGNMEVYIRDIHSGERLGPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSKTEKI-QTILGGIVVIKKGYRIDPEEIRQFVNDRIDDYKHLE-IIILTNEPLPRNPNGKLLRN 540          
BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 159.458 bits (402), Expect = 1.134e-42
Identity = 138/480 (28.75%), Postives = 215/480 (44.79%), Query Frame = 0
Query:  143 LAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKD-FSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLD--LPSPD-----LLTTCMFHISGIGKLLMGPLN--QEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFLNCYSMTEL--GFLVANSMDPHCLGSLAEGAQVKIQDPVTQTALGPLQTGEILV-KPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDL-PEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRN 608
            LAK     G+++ D+V + I N  +    V  T +C  + S++   +S  TL  ++ DT+A+I+ CT+       K +K  + G  I+ +D    ++EM   G +  S+++          P +D FSL D   I WSSGTTG PKG+ ++          S LD    +P      LL+    HISG    L    +      ++  SD +K   ++          K+F     C                                        PN      Y  TE   GFLV  S + H  G +    +  I+D  T   LGP + GE++  K      GY N+ EE  K  + DG+ HTGDF HY E+G  +   R+ E+IK + +HVYP+ELE L++ +  ++ EVGV    +     L  A++ +    ++  E I+K + + VD YK++ G +  T  PLP+NAMGK +R+
Sbjct:   86 LAKSLYEKGLRKSDVVHLIIGNRADIYLPVFATILCQGVISISVDSISHDTLMTELNDTQAEIVFCTNKT----FKKVK--NCGCTIINMD--TDIEEMIANGCT--SKVIV---------PKQDVFSLKDVFVILWSSGTTGQPKGVQMTV--------VSFLDSMFATPKVKGNMLLSANFGHISGFISSLFWTFHFSTSTFYYISSDDVKHFGVV----------KLFEVLKKCV---------------------------------------PNF-----YGSTESGGGFLV--STNQHSNGGVVARTETYIRDLNTGQHLGPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVVPVVEKKQTVLG-AIIVVKNDKKINVETIRKELNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRS 481          
BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 132.109 bits (331), Expect = 1.186e-32
Identity = 137/562 (24.38%), Postives = 248/562 (44.13%), Query Frame = 0
Query:  105 YFEAIQARI-EKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPD-----PEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPD---------------LLTTCMFHISGIGKLLMGPLNQEAIF----FKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIP--SGLDLSSVIIVMP----IGARVPDAMLKRLNVIFPNMNFLN-CYSMTELGFLV----------------ANSMDPHC--LGSLAEGAQVKI---QD--PVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLLA 611
            + E I  +I EK  +  +  D   G   R G++     ++A   T  G  +GD++ +   N +E+  L LG W CG I    + +L    L+ Q+ ++K KI++C        L  ++ T+   +++++ +    Q+          +++   D L D     P+ V      D  ++ ++S ++G  KGI        H  K+ +    SPD                     FH++G     +G +   +I+    F + + L+       A  D K   +   S +  Q+  LS+ P  S  DLSS+ ++M     + + +   +L R+    P++ ++   Y + E G +                 A+  D H   +G     + +KI   QD  PV     GP Q GEI VK P    GY NK E +A   D+D + HTGD G+YD +G++Y   ++  +I++    V P  LE+ +     + +  V G P+  + ++  A V L PG + TEE +   +   + +++++RGG+ F    +P++   K +R++LA
Sbjct:   61 FHEFILQKIGEKEPDRIWIKDLSIGRMERFGNVHSLVNKVASGLTKNGFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVCDDFNYVDGLDFLENTNMIEKLIIIGNECTAQD----------KVILMTDLLNDDGKKVPQKVIFDWAKDVIYMPFTSTSSGA-KGIL-------HTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYGISIYTLSEFSEQNFLE-------ALVDXKTASI---SCYAWQIRILSQSPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRI----PSIKYVREAYGLNECGLVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVD----GPDQQGEICVKSPQCMLGYLNKEESSA--FDNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQN-IPRDENWKVLRSVLA 583          
BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 119.013 bits (297), Expect = 1.817e-28
Identity = 121/464 (26.08%), Postives = 192/464 (41.38%), Query Frame = 0
Query:  172 VLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQL--LAGCDQLP------DPEPVKDFSLDDT-------AFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPDLLTTCMFHISGIGKLLMG--PLNQEAIFFKDSDLLKDI--TLILKACHDYKCGKVFLGSHHCAQLANLS-EIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFL-NCYSMTELGFLVANSMDPHCLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQD-LPEVAEVGVYGKPDPASQELAVAVV---RLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLL 610
            V+GTW  G+  S+  P  S  T++ Q                   L  +K+     +IVV+ +   + ++ + G+  +  L  + G   L       D E V+ F+ + T       A + +S+         C       H V  +        ++ T  FH  G  + L    P  +  I  +  +   D   T +  A    K   +        ++   S ++ S  DLSSV ++ PIG  +     K+    FP++  +   Y  TE   +     D H LG L    Q++I +P  +  LGP   G+I+VKP Y    Y    E        DGF + GD G+YD  G ++   R K+++K  N+HVYP E+E+ I      V ++GV+G+ D    E    V+    +  G  L     +   A     YK L+GGVR T+A +P+NA GK  R  L
Sbjct:  111 VVGTWFLGSSVSLFQPSSSRETIERQ-------------------LMILKEELNLEEIVVITETFAIPKISNIGVDRIISLTPVPGYMSLQEIFMNDDNEVVEIFNREGTTSLQSEIAGVLYSAENILNSIHYCSYVPFQIHHVIKNYEPSSLRLMILTNFFHPGGFTEGLRNILPQKRTGIILRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFDLSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTETLSISLGISDQH-LGRLFPKYQLRILNPNGE-PLGPNSVGDIVVKPGYGMRRYIGDHEYN---ITQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDKNGIERLSGVIVLHHIEKGLSLQNVLERTFEAYKFPRYKWLQGGVRVTSA-IPRNANGKIFRGRL 549          
BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 100.908 bits (250), Expect = 1.512e-22
Identity = 74/217 (34.10%), Postives = 105/217 (48.39%), Query Frame = 0
Query:  398 PDAMLKRLNVIFPNMNFLNCYSMTELGFLV----ANSMDPHCLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLL 610
            P  + K +N    ++ F   Y MTE   +      + +     G+L      KI D  T   LGP + GE+ V  P    GYY   + T K    +G+ HTGD  +Y++ G  Y+  R+KE+IK +   V P ELE+LI+  P V +V   G PD  + EL  A V +     + E+ I   V E V  +K+L  GV F +  LPKN  GK +R  L
Sbjct:  358 PTLIEKFMNKCDSDLRFQEGYGMTECSPVTHVQPKDGIIYGSCGNLIPNTIGKIIDIDTGKVLGPGENGELCVSGPQIMQGYYKNKKATDKIIQ-NGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYV-VRKNRSVMEQGIIDFVTEKVAPHKKLGAGVMFVDT-LPKNQTGKILRREL 571          

HSP 2 Score: 51.9878 bits (123), Expect = 3.897e-7
Identity = 46/193 (23.83%), Postives = 84/193 (43.52%), Query Frame = 0
Query:  105 YFEAIQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTS---TGAQ-IVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSY 293
            Y + I   +E          G+TG       +   +++       LG K+GD++ + +PN  EF    +G    G   +  +P      +  Q++++ AK ++     + +FL+ IKQ+    +G Q I+V+       + K      +    +  DQ    +P KD        + +SSGTTG PKG+ L++
Sbjct:   62 YADFIWENLEDYKEREALVCGMTGRTYTYEMVHSLSQKFGSALLRLGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMT----IGLFLQNIKQSCEIYSGIQKIIVIGMEETPSDCKSFMEMAIFDDGSFFDQRETIDPHKDI-----LALPYSSGTTGPPKGVALTH 245          
BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000006577 (supercontig:LSalAtl2s:LSalAtl2s360:157972:160449:-1 gene:EMLSAG00000006577 transcript:EMLSAT00000006577 description:"maker-LSalAtl2s360-snap-gene-1.18")

HSP 1 Score: 73.9442 bits (180), Expect = 3.993e-14
Identity = 101/478 (21.13%), Postives = 190/478 (39.75%), Query Frame = 0
Query:  135 DLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTS---------SKVEMFLKAIKQTSTGAQ--IVVLDDAAHVQEMKDAG-LSTLSQLLAGCDQLPDP--EPVKDFSL---DDTAFICWSSGTTGTPKGI---CLSYQLVKHRVKTSLLDLPSPDLLTTC---MFHISGIGKLLMGPL--NQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSE-IPSGLDLSSVIIVMPIGARV-PDAML-KRLNVIFPNMNFLNCYSMTELGFLVANSMDPHCLGSLAEGAQVKI--QDPVTQTALGPLQTGE----ILVKPPYP--FPGYYNKPE--ETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQK 574
            DL  +  + + +  S GV++GD V I +P  +E    +L     GA+ S+     S  +L +++ D  AK++I            + +++  KA+++TS+     I +    +H+  +     L T S+     D       EP  D      +D  F+ ++SG+TG PKG+      Y +        + D      +  C   +  I+G   +  GPL    +++ F+ +          +    Y   K +        L    E   S  DLSS+ ++  +G  + P+A      NV     + ++ Y  TE G  +   + P C  +    A        PV     G + +GE    I+   P+P      Y   E  E   F    G+  +GD    D+ G +++ GR+ +++    + +   ++E+ + +   VAE  V   P P   E     +  +    L ++ +++
Sbjct:   62 DLHMEVCKFSNVLKSKGVQKGDRVAIYMPMIIELVVAMLACARIGAVHSIVFGGYSSDSLASRIVDGGAKVLISADGVYRGNKLINLLDVAYKAMEETSSNYNHDIDIHILVSHIPRLNSGSPLETPSRWKTERDHWWHEVMEPASDVEWMNSEDPLFLLYTSGSTGKPKGVLHTTAGYMIYAATTFKYVFDYQDESDVYWCTADIGWITGHTYVTYGPLLNGSKSVLFEGTPFYPTNDRFWEIIQKYSVSKFYTAPTAIRALMKFGEDFVSKYDLSSLKVLGTVGEPINPEAWFWYHKNVGGSQCSIVDTYWQTETGGHIMTPL-PGCTPTKPGSACFPFFGVSPVILDESGKVLSGEAEGYIVFNKPWPGMMRSVYGNHERFEKTYFSRFPGYYMSGDGAKRDKDGYIWITGRIDDMLNCSGHLMSTAQVESALVEHRHVAEAAVVSTPHPIKGECLYCFITPMADISLNDKMVKE 538          
BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000009904 (supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-1 gene:EMLSAG00000009904 transcript:EMLSAT00000009904 description:"maker-LSalAtl2s645-snap-gene-2.24")

HSP 1 Score: 65.855 bits (159), Expect = 2.271e-11
Identity = 73/321 (22.74%), Postives = 145/321 (45.17%), Query Frame = 0
Query:  271 DDTAFICWSSGTTGTPKGICLSYQLVKHRVK--TSLLDLPSPD--LLTTCMFHISGIGKLLMGPLN--QEAIFFKDSDLLKDITLILKACHDYKCGKVFLG---------SHHCAQLANLSEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFLVANSMD-----PHCLGS----------LAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDG-FCHTGDFGHYDEKGMLYLDGRMK-EVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVV 559
            +D A I ++SGT+G+PKG+ ++++ ++ +V          S D  L    ++H+ G+   L+ PL+           D  K  + +  +  +     VF+G           +     N +EI   L  S+  +++   A +P+ +L++   I  ++  L  Y MTE+G  + NS++     P  +G+            EG+ +   + V  + L   ++GE+ ++ P  F  YY   + T +  D +G +  TGD    + +G+  + GR   ++IK     +  +E+E      P+++E+ V G  D    E+  A++
Sbjct:  147 NDGALILYTSGTSGSPKGVWITHRNIEAQVSGMIKFWAWTSDDHILHVLPLYHLHGLINCLLVPLSIGARVKMLPGFDAKKVWSYLTNSSMNIS---VFMGVPTIYSLLTKEYELDKRNKTEIHDMLR-STYRLMVCGSASLPEPLLRKWQTISGHI-LLERYGMTEIGMGLGNSLEEDKRLPGFVGTPFPNVEARIMSEEGSVLAYSNDVEFSYLSD-KSGELQIRGPSVFSFYYGNEKATKESFDPNGDWFLTGDTASMNSRGVFKILGRTSADIIKSGGFKLSALEIEREYLSYPDISEIAVVGLEDEIFGEIVAAII 461          
BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 64.6994 bits (156), Expect = 5.497e-11
Identity = 38/128 (29.69%), Postives = 66/128 (51.56%), Query Frame = 0
Query:  458 PLQT-GEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYK 584
            P+ T GE+ V+ P    GYY   + + K    D + H+GD G   EKG   + GR+ +++     ++YP E+E+++   P + EV + G PD    E   A + L  GA+  E  +++H    + ++K
Sbjct:  388 PVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEELCAWICLEEGAK--EFDLKEHCKGKISHFK 513          

HSP 2 Score: 64.3142 bits (155), Expect = 7.485e-11
Identity = 38/128 (29.69%), Postives = 65/128 (50.78%), Query Frame = 0
Query:  458 PLQT-GEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYK 584
            P+ T GE+ V+ P    GYY   + + K    D + H+GD G   EKG   + GR+ +++     ++YP E+E+ +   P + EV + G PD    E   A + L  GA+  E  +++H    + ++K
Sbjct:  160 PVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEELCAWICLEEGAK--EFDLKEHCKGKIAHFK 285          

HSP 3 Score: 61.2326 bits (147), Expect = 5.659e-10
Identity = 37/128 (28.91%), Postives = 64/128 (50.00%), Query Frame = 0
Query:  458 PLQT-GEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYK 584
            P+ T GE+ V+ P    GYY   + + K    D + H+GD     EKG   + GR+ +++     ++YP E+E+ +   P + EV + G PD    E   A + L  GA+  E  +++H    + ++K
Sbjct:  759 PVNTPGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEELCAWICLEEGAK--EFDLKEHCKGKIAHFK 884          
BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000004507 (supercontig:LSalAtl2s:LSalAtl2s235:163315:165883:-1 gene:EMLSAG00000004507 transcript:EMLSAT00000004507 description:"maker-LSalAtl2s235-augustus-gene-1.27")

HSP 1 Score: 51.6026 bits (122), Expect = 5.229e-7
Identity = 94/429 (21.91%), Postives = 171/429 (39.86%), Query Frame = 0
Query:  214 EMFLKAIKQTSTGAQIVVL---DDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLS---YQLVKHRVKTSLLDLPSPDLLTTC--MFHISGIGKLLMGPL--NQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSE-IPSGLDLSSVIIVMPIGARVPDAMLKRLN--VIFPNMNFLNCYSMTELGFLVA----NSMDPHCLGSLAEGAQVKIQDPVTQTALGPL-----QTGEILVKPPYP--FPGYYN--KPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVV---RLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLLATI 613
            E+  +AIK++S   + V++       HV+ MK      L   +   +    PE +   S +D  F  ++SG+TG PKG+  S   Y L        + D    D+      +  I+G   ++ GPL     ++ F+ +    D +   K   +    + +       QL    +      D SS+  +  +G  +        N  V     + ++ +  TE G +      + ++   + +L     + I +PV     G       + G + +K P+P      Y+  K  +   F    G+  TGD  +  ++G + + GR+ +VI    + +   E+E+++     + E  V G P     E   A V    L  G +  E+ IQK V +N+  +  +    +F    LPK   GK +R +L  I
Sbjct:  145 EVVDEAIKKSSQPIKHVLVAKRSGKGHVK-MKSGRDVYLEDAMLQVESECHPESM---SSEDPLFYLYTSGSTGAPKGLVHSTAGYLLYTSFTHKIIFDFCEHDIFGCVADIGWITGHSYIVYGPLMNGGTSVLFESTPTYPDPSRYWKLVEELGITQFYTSPTAIRQLMKYEDSYVLNSDKSSLRTLGSVGEPINIEAWNWYNDLVGRNRCDIVDTWWQTETGGICITPRPSELNAEIVPALGMRPFLGI-NPVLLDDKGNEIKENNKQGSLCLKTPWPGMARSIYDDHKRYQETYFSAFPGYYFTGDGAYRTKEGYISIVGRIDDVINVSGHRLGTAEVEDVLTKHDNIVEAAVVGXPHDIKGEAIYAFVVPKNLDEGVKKFEKGIQKLVKDNISGF-AVPEKFQFCE-DLPKTRSGKIMRRILRKI 566          
BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|75290242|sp|Q6ETN3.1|4CL3_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; Short=Os4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 185.267 bits (469), Expect = 4.628e-50
Identity = 160/530 (30.19%), Postives = 255/530 (48.11%), Query Frame = 0
Query:  111 ARIEKVGNARYC-TDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIII---CTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPDL------LTTCM---FHISGIGKLLMGPLNQEA--IFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIP--SGLDLSSVIIVM----PIGARVPDAMLKRLNVIFPNMNFLNCYSMTELG----FLVANSMDPH-----CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLL 610
            AR+ +VG AR C  DG TGE     ++E  +RR A     +GV +GD+V   + N  EF+   LG    GA  +  +P  +   +  Q E   A++I+   C   KV  F         G  +V +D A         G     ++LA  +   D     D   DD   + +SSGTTG PKG+ L+++ +   V   + D  +P+L      +  C+   FHI  +  +L+  L   +  +  +  DL   + L+ K  H+     + +       +  +++ P  +  DL+S+ +VM    P+G  + DA + ++    PN      Y MTE G      +A + +P        G++   A++KI DP T T+LG  Q+GEI ++      GY N PE T    D DG+ HTGD G  D+   +++  R+KE+IKY+   V P ELE L+   PE+ +  V    D  + E+ VA +    G+++TE+ I+K VA+ V  YK++   V FT++ +PKN  GK +R  L
Sbjct:   33 ARMAEVG-ARPCLIDGQTGESYTYAEVESASRRAAAGLRRMGVGKGDVVMSLLRNCPEFAFSFLGAARLGAATTTANPFYTPHEVHRQAEAAGARVIVTEACAVEKVREF-----AAERGVPVVTVDGAFD-------GCVEFREVLAAEELDAD----ADVHPDDVVALPYSSGTTGLPKGVMLTHRSLITSVAQQV-DGENPNLYFSKDDVILCLLPLFHIYSLNSVLLAGLRAGSTIVIMRKFDLGALVDLVRK--HN-----ITIAPFVPPIVVEIAKSPRVTAEDLASIRMVMSGAAPMGKDLQDAFMAKI----PNAVLGQGYGMTEAGPVLAMCLAFAKEPFKVKSGSCGTVVRNAELKIVDPDTGTSLGRNQSGEICIRGEQIMKGYLNDPEATKNTIDEDGWLHTGDIGFVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDDLAGEVPVAFIVRTEGSEITEDEIKKFVAKEVVFYKRIN-KVFFTDS-IPKNPSGKILRKDL 531          
BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|75311763|sp|Q9M0X9.1|4CLL7_ARATH (RecName: Full=4-coumarate--CoA ligase-like 7; AltName: Full=4-coumarate--CoA ligase isoform 6; Short=At4CL6)

HSP 1 Score: 177.563 bits (449), Expect = 2.687e-47
Identity = 150/535 (28.04%), Postives = 244/535 (45.61%), Query Frame = 0
Query:   97 PDKHMVEVYFEAIQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSL-----DDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPDLLTTC---------MFHISGIGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFLVANSMDPH-------CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLL 610
            P+  +V   F    +   K+  A    D  TG+      L+    RLA  F  LG+++ D+V I  PN  +F    L     G + +  +P  +V  +  Q++D+  KIII     V      IK       ++   D   +    +      S++L+  + +   EPV ++        DTA + +SSGTTGT KG+ L++    + +  SL+     DL+            MFH+ G+  +    L +       +    ++ L+LK    ++   +++       L+  S I    DLSS+  +    A +   +++      PN+  +  Y MTE   +V+   DP          G LA G + +I    T  +  P Q GEI V+ P    GY N P+ T +  D   + HTGD G+++E G LY+  R+KE+IKY+   V P ELE L+   P++ +  V   PD  + E+ +A V   P + +TE+ IQK +A+ V  YK+LR  V F +  +PK+A GK +R  L
Sbjct:   25 PNTSLVSFLFRNSSSYPSKLAIA----DSDTGDSLTFSQLKSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIIS----VNQLFDKIKGFDLPVVLLGSKDTVEIPPGSN------SKILSFDNVMELSEPVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTH---GNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNALVSMARF--ELELVLKNIEKFRVTHLWVVPPVFLALSKQS-IVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVE-DPRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLR-RVSFISL-VPKSAAGKILRREL 536          
BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|158564046|sp|Q84P21.2|4CLL5_ARATH (RecName: Full=4-coumarate--CoA ligase-like 5; AltName: Full=4-coumarate--CoA ligase isoform 9; Short=At4CL9; AltName: Full=Peroxisomal OPC-8:0-CoA ligase 1)

HSP 1 Score: 177.563 bits (449), Expect = 3.068e-47
Identity = 145/503 (28.83%), Postives = 236/503 (46.92%), Query Frame = 0
Query:  124 DGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKD-FSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPD-----LLTTCMFHISGIGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLAN-LSEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFL------VANSMDPHCLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLLATI 613
            D  TG+     +L      +A   + +G+++G +V +  PN + F  + L     GAI + T+P  +   +  Q++D+   +   TS  +     A K+       +VL D   V  + D     + +L+    + P    VK+    DDTA + +SSGTTG  KG+  S++ +   V+T +    S D     + T  MFHI G+     G L   +     S    ++  ++ A   Y+   + L       + N   +I +  DLSS+  V+  GA +   + +     +P +  L  Y +TE   +      V  S      G L+   + +I DPVT   LGP QTGE+ +K P    GY++  E T+   DS+G+  TGD  + DE G +++  R+KE+IKY+   V P ELE L+   PE+ +  V   PD    +  +A V    G+ L+E+ I + VA+ V  YK++R  V F ++ +PKN  GK +R  L  I
Sbjct:   51 DASTGQNLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLP-----IVLMDEERVDSVGD-----VRRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKF--EMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIR-KVAFVSS-IPKNPSGKILRKDLIKI 539          
BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|57012620|sp|Q9LU36.1|4CL4_ARATH (RecName: Full=4-coumarate--CoA ligase 4; Short=4CL 4; AltName: Full=4-coumarate--CoA ligase isoform 5; Short=At4CL5; AltName: Full=4-coumaroyl-CoA synthase 4)

HSP 1 Score: 173.326 bits (438), Expect = 1.300e-45
Identity = 146/507 (28.80%), Postives = 240/507 (47.34%), Query Frame = 0
Query:  124 DGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAH--VQEMKDAGLSTLSQLLAGCDQLPDPEPVK-DFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPDLLTTC---------MFHISGIGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFL---VANSMDPH-----CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLL 610
            DG TG      D++   RR+A     LG++ GD+V + +PN  EF+   L     GA+ +  +P  +   +  Q + + AK+II     V+   K     + G  IV LDD     V    D G  + ++L     Q  + E +K   S +DT  + +SSGTTG PKG+ ++++ +   +   + D  +P+L  T          MFHI  +  L++  +   A          ++ L+++    YK   V +            E     DLSSV I++   A +   +   + + FPN  F   Y MTE G +   +A + +P        G++   A++K+ D  T  +L   ++GEI V+      GY N PE TA+  D DG+ HTGD G  D+   +++  R+KE+IK++   V P ELE L+   P + +  V    D  + E+ VA V    G+QLTE+ ++ +V + V +YK+++  + F    +PK   GK +R  L
Sbjct:   67 DGATGRILTYADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKASAAKMIITKKCLVD---KLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELT----QADETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKV-DGENPNLNFTANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRF--ELNLVMELIQRYKVTVVPVAPPVVLAFIKSPETER-YDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVAKSLAFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIK--MVFFIEVIPKAVSGKILRKDL 560          
BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|148841518|sp|Q9S725.2|4CL2_ARATH (RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName: Full=4-coumarate--CoA ligase isoform 2; Short=At4CL2; AltName: Full=4-coumaroyl-CoA synthase 2)

HSP 1 Score: 171.4 bits (433), Expect = 5.209e-45
Identity = 153/512 (29.88%), Postives = 243/512 (47.46%), Query Frame = 0
Query:  119 ARYC-TDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPDL------LTTC---MFHISGIGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIV----MPIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFLVANSM----DPH-----CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVR 607
            A+ C  +G TGE     D+   +R+LA    +LGVK+ D+V I +PN  E     L     GAI +  +P  +   +  Q + + AK+I+  S  V+     IK       ++V  D+  + E         S+L    +   D  P K  S +D   + +SSGTTG PKG+ L+++ +   V   + D  +P+L      +  C   MFHI  +  +++  L   A          +ITL+L+     K     +       +A   E     DLSSV +V     P+G  + DA+  +    FPN      Y MTE G ++A S+    +P        G++   A++KI DP T  +L   + GEI ++      GY N P  TA   D DG+ HTGD G  D+   L++  R+KE+IKY+   V P ELE+L+   PE+ +V V    +  + E+ VA V     + ++E+ I++ V++ V  YK++   V FT++ +PK   GK +R
Sbjct:   51 AKPCLINGPTGEVYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVTQSRYVD----KIKNLQNDGVLIVTTDSDAIPE----NCLRFSELTQSEEPRVDSIPEK-ISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQV-DGENPNLYFNRDDVILCVLPMFHIYALNSIMLCSLRVGATILIMPKF--EITLLLEQIQRCKVTVAMVVPPIVLAIAKSPET-EKYDLSSVRMVKSGAAPLGKELEDAISAK----FPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRI-NKVFFTDS-IPKAPSGKILR 543          
BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|1168218|sp|P41636.1|4CL_PINTA (RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName: Full=4-coumaroyl-CoA synthase)

HSP 1 Score: 171.014 bits (432), Expect = 5.257e-45
Identity = 149/526 (28.33%), Postives = 249/526 (47.34%), Query Frame = 0
Query:  112 RIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDA-----AHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPDL------LTTC---MFHISGIGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVM----PIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFLVANSM----DPH-----CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLL 610
            R+ +  +     DG T       ++E  +R++A     LG+++G +V + +PN +EF+ + +G  V GAI +  +P      +  Q +   A+II+  ++ VE   K     S    ++ +DDA      H+  + +A  +                P      DD   + +SSGTTG PKG+ L+++ +   V   + D  +P+L      +  C   +FHI  +  +L+  L   A          ++T  L+    YK     +       +   S I S  D+SSV I+M    P+G  + DA+ +R    FP   F   Y MTE G ++A ++    +P        G++   AQ+KI D  T  +L   Q GEI ++ P    GY N PE TA   D +G+ HTGD  + D+   +++  R+KE+IKY+   V P ELE L+   P +A+  V  +    + E+ VA V  V  ++++E+ I++ VA+ V  YK++   V F +A +PK+  GK +R  L
Sbjct:   34 RVAEFADRPCLIDGATDRTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARIIVTLAAYVE---KLADLQSHDVLVITIDDAPKEGCQHISVLTEADETQC--------------PAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQV-DGENPNLYFHSDDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKF--NLTTCLELIQKYKVTVAPIVPPIVLDITK-SPIVSQYDVSSVRIIMSGAAPLGKELEDALRER----FPKAIFGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVEYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEEAGEVPVAFV--VKSSEISEQEIKEFVAKQVIFYKKIH-RVYFVDA-IPKSPSGKILRKDL 530          
BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|158564049|sp|Q84P25.2|4CLL2_ARATH (RecName: Full=4-coumarate--CoA ligase-like 2)

HSP 1 Score: 171.4 bits (433), Expect = 5.351e-45
Identity = 145/502 (28.88%), Postives = 231/502 (46.02%), Query Frame = 0
Query:  124 DGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKD-FSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKT--SLLDLPSPDLLTTCMFHISGIGKLLMG--PLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLAN-LSEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFLVANSMDPH------CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLLATI 613
            D +TG      +L     R+A    +LGV++G++V I  PN + F  + L     GAI +  +P  +   +  Q+ D++  +   T   V     A   ++    +V++DD     +     +  + +L    +  P    VK   + DDTA + +SSGTTGT KG+ LS++ +   V+   +   L    + T  M HI G G    G   L    +     D+ K    +L A   ++   + L       + N  +EI S  DLSS+  V+  GA +   + ++    +P +  L  Y +TE   + A+  +          G LA   + KI DP T   LG  QTGE+ ++ P    GY+   E TA   DS+G+  TGD  + D  G +++  R+KE+IK     V P ELE L+   PE+A+  V   PD  + +  +A +    G+ L+E  I   VA+ V  YK++R  V F  A +PKN  GK +R  L  +
Sbjct:   68 DAVTGRRLSFPELWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGDSRPVLAFTTCKLVSKLAAA---SNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRTICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAK----LLSAVETHRSSYLSLVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIR-KVTFL-ASIPKNPSGKILRRELTKL 560          
BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|12229631|sp|O24145.1|4CL1_TOBAC (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 166.777 bits (421), Expect = 2.097e-43
Identity = 151/519 (29.09%), Postives = 246/519 (47.40%), Query Frame = 0
Query:  118 NARYC-TDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTST--GAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSL------LDLPSPDLLTTCM--FHISGIGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIP--SGLDLSSVIIVM----PIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFLVANSM----DPH-----CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLL 610
            ++R C  +G   +     ++E   R++A     LG+++ D + I +PN  EF    +G    GAI +M +P  +   +  Q + + AKIII  S     F+  +K  ++    +++ +D A         G    S+L    D+   PE VK    DD   + +SSGTTG PKG+ L+++ +   V   +      L + S D+L   +  FHI  +  +L+  L   A          DI   L+    YK   V +G      +  +++ P     DLSSV  VM    P+G  + DA+  +    FPN      Y MTE G ++A  +    +P        G++   A++KI DP T  +L   Q GEI ++      GY N PE T +  D +G+ HTGD G  DE   L++  R+KE+IKY+   V P E+E L+ + P +++  V    D  + E+ VA V    G+ +TE+ ++  +++ V  YK+++  V F    +PK+  GK +R  L
Sbjct:   44 SSRPCLINGANDQIYTYAEVELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKIIITQSC----FVGKVKDYASENDVKVICIDSAPE-------GCLHFSELTQS-DEHEIPE-VK-IQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSILLCGLRVGAAILIMQKF--DIAPFLELIQKYK---VSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVMSGAAPLGKELEDAVRTK----FPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVK-RVFFVET-VPKSPSGKILRKDL 537          
BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|112800|sp|P14912.1|4CL1_PETCR (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 166.777 bits (421), Expect = 2.190e-43
Identity = 154/526 (29.28%), Postives = 247/526 (46.96%), Query Frame = 0
Query:  113 IEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIII---CTSSKVEMFLKAIKQTSTGAQIVVLDDAA----HVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSL------LDLPSPDLLTTCM--FHISGIGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIP--SGLDLSSVIIVM----PIGARVPDAMLKRLNVIFPNMNFLNCYSMTELG----FLVANSMDPH-----CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRN 608
            I KVG+     +G TGE      +E  +R++A     LG+++GD + + +PN  E+    LG    GAI +M +P  +   +  Q++ ++AK+II   C   KV+ +           QI+ +DDA     H  ++ +A  S + +++               + DD   + +SSGTTG PKG+ L+++ +   V   +      L + S D++   +  FHI  +  +L   L              DI   L+    YK   V +G      +  +++ P     DLSSV  VM    P+G  + DA+  +    FPN      Y MTE G      +A + +P+       G++   A++KI DP T  +L   Q GEI ++      GY N PE T    D +G+ HTGD G  D+   L++  R+KE+IKY+   V P ELE L+   P +++  V    D  + E+ VA V    G   TEE I++ V++ V  YK++   V F +A +PK+  GK +R 
Sbjct:   36 ISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKLIITQACYVDKVKDY-----AAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV--------------INSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCGLRAGVTILIMQKF--DIVPFLELIQKYK---VTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSGAAPLGKELEDAVRAK----FPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIF-RVFFVDA-IPKSPSGKILRK 531          
BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|75296548|sp|Q7XXL2.2|4CLL9_ORYSJ (RecName: Full=4-coumarate--CoA ligase-like 9)

HSP 1 Score: 166.392 bits (420), Expect = 2.958e-43
Identity = 149/539 (27.64%), Postives = 241/539 (44.71%), Query Frame = 0
Query:   93 PVTAPDKHMV-EVYFEAIQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQL-PDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPD-------LLTTCMFHISGIGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANL-SEIPSGLDLSSVII--VMPIGARVPDAMLKRLNVIFPNMNFLNCYSMTELG--FLVANSMDPH-------CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLL 610
            PV  PD   V E   +  +A  ++V        G +  +   G++     R A+   S+GV++G +V +++PN   +  + LG    GA+ S  +P      ++ QVED++AK+++      +      K    G  ++ + D   +      G  +   LLA  D+      PV      D   + +SSGTTG  KG+ LS++ +   + +S+  + +P+       L     FHI GI  +    L  +           D+   L+A  D++   +F        LA + S +    DLS + +  VM   A +   +L      FP +     Y +TE     L   + D H        +G +    +VK  DP T  +L     GE+ V+      GYY + EET +  D  G+ HTGD G+ D  G +++  R+KE+IKY+   V P ELE ++   P V +  V+G PD  + E+ VA V    GA+  EE I  +VAE V +YK++R  V      +PK+  GK +R  L
Sbjct:   26 PVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTY---GEVARDTARFARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSEAKLVVANEVAFD------KVKDAGVPVIGVGDRERMP-----GAISWDGLLAAADRTGAGVVPVDAAQQSDLCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAV-APETAGQVVTLGLMPFFHIYGITGICCATLRHKGTVVVMDRF--DLRTFLRALVDHRV--MFAPLVPPVMLAMVKSPVADEFDLSDLALKSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGFILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVGYIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVACVVRRHGAEEGEEEIVAYVAERVASYKRVR--VLHIVDAIPKSVSGKILRRQL 543          
BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1325285932|ref|XP_023327697.1| (4-coumarate--CoA ligase-like [Eurytemora affinis])

HSP 1 Score: 225.328 bits (573), Expect = 2.433e-62
Identity = 155/508 (30.51%), Postives = 251/508 (49.41%), Query Frame = 0
Query:  109 IQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQ---IVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPDLLTTCMFHISGIGKLLM----GPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFL-NCYSMTELGFLVANSMDPHCLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAV-AVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVR 607
            IQA + + G+  +  D  T    RI  +  ++ ++A   T LG K GD +  +    L+F   + G W+CG + S+ DP LS + L++Q++DT AKII+C       FL+A +   +  Q   I+VLD     Q + +   S  S      DQ PD   +  +   +   I W+SGTTG+PKG+ +S + +  +++ S L   +  L +   FH    G  L+    G  N+ +I F        +  I +    YK      G++   QLA  S   +  DLSSV ++MP G ++  +   R+   FPN+ F+   Y  TE+G  V+ S     +G+L EG Q  I+D  T   LG  + GEI+ K P     Y N+ +E+ +  D +GF H GD G+YDE G+L+   R+KE+IK  N  + P E E+ ++++P V E  V+   +  +    +   V L   + L  E I +   ++V+  +++ G +   N  +P N  GK +R
Sbjct:   32 IQAELRETGDRDWILDCTTENSVRISHILGRSIKVASGLTRLGFKPGDTLHTAYSTCLDFYWPIFGAWLCGGVVSVGDPGLSASNLKSQIQDTHAKIIVCEVESCSKFLEANQSLLSAEQASNILVLD-LEPWQPIPEGTSSFRSLYEDQGDQCPDRASLPGYIPKEPVVIHWTSGTTGSPKGVVMSQEYLHFQMRQSKLPEKTIGLASNTFFHA---GSFLLSLDGGIRNKFSIHFIQPKHTT-VESIFRFIEKYKPMFFMCGANDFVQLA--SSDLAKFDLSSVNVIMPAGNKIAKSAATRMKEKFPNLKFVYQFYGSTEVGG-VSGSFTVENMGALREGVQTYIRDQKTGEKLGVGEVGEIMAKTPTMMIHYLNRQDESRELLDEEGFAHMGDLGYYDENGILHYKERIKEMIKIGNYWIGPAEFEDNLENIPGVVEAVVWQSMNKETDNYYIHGAVTLQASSGLNPEKILELANKDVEVKRRIDGTIHIINN-MPHNPQGKKLR 530          
BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1325261085|ref|XP_023330441.1| (4-coumarate--CoA ligase 1-like [Eurytemora affinis])

HSP 1 Score: 219.55 bits (558), Expect = 3.225e-60
Identity = 158/535 (29.53%), Postives = 259/535 (48.41%), Query Frame = 0
Query:   97 PDKHMVEVYFEAIQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQ---TSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDL-------PSPDLLTTCMFHISGIG-KLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVMPIGARV-PDAMLKRLNVIFPNMNFLNCYSMTELGFLVANS---MDPHCLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDP--ASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLLATIM 614
            P++  +E     I   ++  G+  +  D  TG    I  +EP +RR+A    SLG   GD++Q    + L+F   V G W+CG   S+ DP+LS T ++ Q+E+TKAK+++ +   ++ + K I++        +  +LD  A  Q+    G+ +   L+   +    P  + DF+ D  + I WSSG++GTPKGI  S        + +LL+L       P   + +  MFHIS     L +G L     +           L LK     +   VF G     Q++N     +G +L+ V  + P+G  V P    K L+++      +  Y  TE+ F+  +    ++   LGSLA G ++ IQD  T   L P + G+I+VK       Y N+ +ET +F DSDGF  TGD G+YD  G ++   RM+EV+K  N    P E+ENL++  P + E  V+G+ DP   + ++ + +V+     + T+  I   V  N+   +++ G + F    +P N  GK +R  +   M
Sbjct:   20 PNQPYIERLLSEINQELDDTGDRDWLVDTETGSSRTISQIEPISRRVASALLSLGFGPGDVLQTGYSSCLDFYWPVFGAWLCGGTVSLGDPNLSETNIKKQLEETKAKVLVSSIDYLDKYYKVIQELKLQGKNVKYFILD--AGEQDTLPLGVESFQTLMRVQETQKMP-LLLDFTPDRESIIMWSSGSSGTPKGILHS--------QKNLLNLYFGHDLKPKTIISSALMFHISSFTFNLTLGILKGTICYIMKEKCFSGENL-LKVAEKIQPEHVFCGVSQYIQVSNTK--TNGYNLNGVKNITPLGGAVSPGCSEKVLSLLGSQATLVEMYGSTEVMFVSKHEVKDIEFGLLGSLAAGVEICIQDINTGERLDPGKEGKIMVKTNTMMMRYLNREKETNEFFDSDGFGFTGDIGYYDNFGKIFFSYRMREVLKVDNYWFGPAEIENLLEKEPTIDEACVWGEYDPNTGNDQVNLGIVQKQNQKEWTKAKICSLVDANLPCTRKITGKIYFVEK-IPHNPQGKKLRGEMKNFM 539          
BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1325319021|ref|XP_023341279.1| (4-coumarate--CoA ligase 1-like [Eurytemora affinis])

HSP 1 Score: 217.624 bits (553), Expect = 2.102e-59
Identity = 149/524 (28.44%), Postives = 260/524 (49.62%), Query Frame = 0
Query:  102 VEVYFEAIQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFL---KAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPD--LLTTCMFHISGIGKLLMGPLNQEAIFF--KDSDLLKDITL----ILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVMPIGARV-PDAMLKRLNVIFPNMNFLNCYSMTELGFLVANSMDPH----CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPD--PASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVR 607
            +E     +   IE+ G+  +  D  TG+ + I  +EP +RR+A    +LG   GD++Q    + L+F     G W+CG   S+ DP+LS T ++ Q++DTKAKII+C+   ++ +    + +++      + VLD+         A + +    L     +   E + ++  D    + WSSG+TG PKGI  S++ ++     SL    SP   L++  MFHI G        LN     F    S  +K+         K    ++   V++G     Q++    +    +LS V ++ P+G  V P+   K L+++      +  Y  TE+ FL A  ++ H     LG+L  GA++ IQD  T   LGP + G+++V+       Y N+ EE  +F +SDGF + GD G+YDE+G ++   RM+EV+K  N    P E+ENL++ L ++ E  V+G+ D    + ++++ +V      + TE  I +++  N+   +++ G +   +  +P N  GK +R
Sbjct:   25 IERLLAEVSQEIEETGDRDWIVDTETGQSHTISKIEPVSRRVAGALHNLGFGPGDVLQTGYSSCLDFYWPAFGAWLCGGAVSLADPNLSSTAIKQQIKDTKAKIIVCSKQYLDKYCAIKQELREEDVHFNLSVLDEE---DGNLPADVKSFQSFLNFDGDISHNEKL-EYIPDSVTMVLWSSGSTGNPKGILHSHRTLQ---TISLAKPSSPKSILVSAIMFHIGGF------TLNLSIALFGGTTSYFIKEKCFSGENWFKIAEKFQPEHVYIGVSQFIQVSKAKNVDR--NLSGVKMITPLGGAVSPECCEKVLSMVGDQAKIIEMYGSTEIVFL-AFKLNKHPKFGFLGTLGSGAEMYIQDIKTGEKLGPRKEGKMMVRTRSMMMNYLNREEENREFFNSDGFGYVGDVGYYDEEGNIFFCYRMREVLKVDNYWFGPEEIENLMEALDDINEACVWGEYDMNTGNDQVSLGIVLQANKEEWTETRINEYITSNLPITRRITGKIYLLDR-IPHNPQGKKLR 531          
BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|390364692|ref|XP_786981.3| (PREDICTED: 4-coumarate--CoA ligase 1 isoform X1 [Strongylocentrotus purpuratus])

HSP 1 Score: 213.772 bits (543), Expect = 3.857e-58
Identity = 160/518 (30.89%), Postives = 250/518 (48.26%), Query Frame = 0
Query:  109 IQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKT-----SLLDLPSPDLLTTCM--FHISGIGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVM----PIGARVPDAMLKRLNVIFPNMNFLNCYSMTELG---FLVANSMD-PHCLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQ-LTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLL 610
            I  + E+ G++    D  +G       +   +RR+A   +  G+K+GD++ I  PN  E+  ++ G    G I S  +P  +   L  Q+E   A++I+      E  L  +K+      + V  +A   +  K    S L+   + C       P    SL+DT  + +SSGTTG PKG+ L+ + +   ++       L D    D +   +  FH  G+  +++  L + A     S    ++   LK   DYK   ++L       LA    +    DLSSV +++    P+G  +  ++  RL +          Y +TE G    L  +S D P  +G L    + K+ D V+   LG  Q GE+L + P   PGY N PE TA+  D+DGF HTGD GHYD+ G+ Y+  R+KE+IKY+   V P ELE L+   P + +  V G P+  + EL  A +  VP  Q LT + + + VA+N   YK+LRGGV F  + +PK+A GK +R +L
Sbjct:   23 IYEKFEQFGDSTAVADAASGRSYTFAQVRAYSRRIASALSRQGIKKGDVIGIVSPNLPEYVLMLCGVVEMGGIVSGVNPLYTEDELLHQMETVDARLIVTVPPFAEKCLNTMKRFPRVQDVYVFGEAEGCKPFK----SLLADDGSAC-------PDVKVSLEDTFALPFSSGTTGLPKGVILTNKTIVSNLRQLESHPDLTDTRPGDTVLALLPYFHCYGLVVIMLHGLRKGARQVTMSRFEPEV--FLKTIQDYKVNHLYLVPPIMLFLAK-HPVVDKFDLSSVSLIISGAAPLGGELTASLKTRLGIKVIKQG----YGLTESGPVLTLSPSSTDVPSSVGKLLPNTEAKVVDTVSGELLGEGQDGELLFRGPQIMPGYLNNPEATARTLDADGFLHTGDIGHYDQDGLFYIVDRLKELIKYKGYQVAPAELETLLLTHPSIMDAAVIGVPNEEAGELPKAFI--VPKNQELTADQVAEFVADNAAPYKKLRGGVEFVKS-IPKSASGKILRRVL 519          
BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1325317676|ref|XP_023340582.1| (4-coumarate--CoA ligase-like 7 [Eurytemora affinis])

HSP 1 Score: 209.92 bits (533), Expect = 1.195e-56
Identity = 145/534 (27.15%), Postives = 256/534 (47.94%), Query Frame = 0
Query:   84 HDPLNNADNPVTAPDKHMVEVYFEAIQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAI---KQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPDLLTTCMFHISGIG-KLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVMPIGARV-PDAMLKRLNVIFPNMNFLNCYSMTELGFL--VANSMDPHC-LGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDP--ASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVR 607
            + PL     P   P+K  ++     ++  +++ G+  +  D  T     I  +EP +RR+A    S G +  D++Q+   + L+F   V G W+CG + SM DP+L    ++ Q+ DT+AK+++C+   ++ ++K +   ++     ++ VLD    V  +   G+     LL    +  D   +  +  +  A I WSSG+TG PKGI  S QL  + +       P   LL+  MFHI G    +L+        +F   +   +  +  K+   ++    F G     +L+N+      LD   + ++ P+G  V     L  LN++  +++ +  Y  +E+ F+   +N     C LG L  G Q+  +D  T   L P + G+I++K       Y N+ +ET +F  SDGF + GD G+YD  G +Y   RM+EV+K       P E+EN+++ + E+ E  V+G  DP   + ++ V +V      + T   I+K+V +N+   + + G +    + LP N  GK +R
Sbjct:   10 YSPLGPKAKP---PNKSFIDRVLSHVKHELKETGDRDWLVDTETELTISISQIEPTSRRVAAALYSHGFRADDVLQMGYSSCLDFYWPVFGAWLCGGVASMGDPNLEEMAIKQQILDTRAKVVVCSKQYLDKYVKVLLELRKEECNLKLFVLDAGKEV--ILPDGVEHFQNLLE-FKENNDFVGLHSYDPNSEALIIWSSGSTGVPKGIVFS-QLGLNNISLGKQYQPKKILLSLIMFHIGGFSVSVLLSIFEGAVCYFIKKECFSE-EVWFKSVDKFQLEHAFCGISQYTRLSNVKIDVKPLD--GLKVITPLGGSVTSQGTLNLLNLVGHHVSIIEMYGSSEVTFISSYSNKEFKSCVLGVLDPGVQMYFEDMETGVKLEPGKEGKIMIKNESMMLYYLNREKETKEFYSSDGFGYMGDVGYYDATGAIYYSYRMREVLKVSGCWFGPAEIENVLEKVNEIDEAFVWGDYDPNTGNDKVNVGIVLRKGIVEWTATNIKKYVEDNLPESRHITGKLYIVES-LPHNPAGKKLR 532          
BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1330905577|gb|PNF29545.1| (Luciferin 4-monooxygenase [Cryptotermes secundus] >gi|1330905578|gb|PNF29546.1| Luciferin 4-monooxygenase [Cryptotermes secundus] >gi|1330905579|gb|PNF29547.1| Luciferin 4-monooxygenase [Cryptotermes secundus] >gi|1330905580|gb|PNF29548.1| Luciferin 4-monooxygenase [Cryptotermes secundus] >gi|1330905581|gb|PNF29549.1| Luciferin 4-monooxygenase [Cryptotermes secundus] >gi|1330905582|gb|PNF29550.1| Luciferin 4-monooxygenase [Cryptotermes secundus])

HSP 1 Score: 207.608 bits (527), Expect = 6.727e-56
Identity = 168/549 (30.60%), Postives = 260/549 (47.36%), Query Frame = 0
Query:   81 LTSHDPLNNADNPVTAPDKHMVEVYFEAIQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLD-DAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQ----LVKHRVKTSL--LDLPSPDLLTTCMFHISGIGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVM----PIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFLV----ANSMDPHCLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLLATIM 614
            + S   L+  +  +T PD  +    ++++     + GNA    D +TGE     D+  ++  +A+     GV  GD+V I   N L+F   VL T+  GAI +  +P+ +   +   +  +K +II C+   +    +  +      +IV      +H    K     T  +     D+    +P+ +  ++  A I  SSGTTG PKG+ LS +    L+++  + S+  +D     L  T MFH  G   +L+       +   D     +  + L++  ++K   +FL       LA  +EI    DLSSV  +     P+   +   + KRL V       +  Y +TE    V     N       G+L  G   K+ D  T   LGP +TGE+  K P+   GYY  P+ TA   DSDGF  TGD G+YDE G  Y+  R KE+IKY+   V P ELE ++   P V + GV G PD A+ EL +A +   PGA LT+E I K+VA++V   K+LRGGV F  + +PK + GK +R  L  ++
Sbjct:    1 MASQHILSGPEISITVPDISVGRYLYQSLV----RHGNAVAMVDAVTGETRTFSDILTRSLSVAECLRVRGVAIGDVVAICSENSLDFIVPVLATYYIGAICAPMNPNYTAHEMLHVINISKPRIIFCSGKALACVDEVSRHVGFLKEIVTFGLPMSH----KHTPFVTFLR-----DKSCSFQPLDEHDMNLVAAILCSSGTTGLPKGVMLSQKNIISLLEYVSEPSINFIDNREVTLGLTPMFHAYGFMFMLLLATAGSKVVVMDR---FEEHIFLRSIQNHKIKHLFLVPPLVVFLAK-AEIVDKYDLSSVQRIYSGAAPLSEEIETLVKKRLGVDL----VIQAYGLTETTLAVIGPTENENKKGTAGTLRPGVTCKVVDLETGRTLGPHKTGELRFKGPFIMKGYYGDPQATAASFDSDGFLLTGDVGYYDEDGFFYIVDRAKELIKYKGYQVPPAELEAVLLTHPAVKDAGVVGIPDEAAGELPLAFIVKQPGATLTKEEIIKYVADHVSPQKRLRGGVLFVES-IPKTSSGKILRRELRIML 527          
BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|909562920|ref|XP_013136653.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Papilio polytes])

HSP 1 Score: 206.453 bits (524), Expect = 1.854e-55
Identity = 159/504 (31.55%), Postives = 252/504 (50.00%), Query Frame = 0
Query:  124 DGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTL---SQLLAGCDQLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLSYQLVKHRVKTSLLDLPSPDLLT-TCMFHISGIGKLLMGPLNQEAIFFKDSDLLK-DITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVM----PIGARVPDAMLKRLNVIFPNMN-FLNCYSMTELGFLVANSMDPH---CLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGKPVRNLLATIM 614
            +G T E    G++  +A  LA     +GVKRGD++ I   N  EF S V+G    G + +  +   +   L+  +  +K K +IC+    +   K  K       +++  +      +    L+ +   + +L    ++ + E ++    DD A I +SSGTTG PKG+ +++  V      ++ ++P    LT T  +H  G+   L+G +  +   +    L K ++   L+    YK  ++   S     L  L + P+  D+SSV+IV+    P+     DA+LKR    FPN    L  Y MTE    V+ + +P     +G +   + VKI  P T   LGP Q GE+ VK      GY  K ++     D +GF  TGD G+YDE   LY+  R+KE+IKY+   V P E+E ++     V +VGV GKP P++ E+ VA V   P +++TE  +Q+ VAE + N K LRGGVRF +A +PK+A GK +R  L  ++
Sbjct:   41 NGATDEKLTYGEIAQEAMNLAVSLVKIGVKRGDVIAICSENRREFWSSVIGVICAGGVVTTINTSYTKDELKHVMGISKPKFVICSPFSYKAQEKNFKNIEGIKNLIIFGEEIPKNTLSYNALAIVGGSNSMLKENVKIDEFEAIEVVGKDDLAMILYSSGTTGLPKGVMITHLNVVTACSPTM-EMPDIVALTITPWYHTMGLMSHLIGFVKGKMSIY----LPKFEVEQYLRTIEKYKVSQL---SVVPPVLVALCKSPTKHDVSSVMIVVSGAAPLHKETADAVLKR----FPNAQAVLQGYGMTEATLAVSINTNPDKKASVGQVTSNSVVKIVHPETGKVLGPNQEGEVRVKGVMLMKGYIGKKKQDD--FDEEGFFKTGDIGYYDEDKYLYIVDRLKELIKYKAYQVAPAEIEAVLLKHEGVRDVGVVGKPHPSAGEVPVAFVVTQPDSKVTEAELQQFVAERLSNTKHLRGGVRFIDA-IPKSATGKILRKDLKKMV 529          
BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|801398403|ref|XP_012059653.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Atta cephalotes])

HSP 1 Score: 204.142 bits (518), Expect = 9.921e-55
Identity = 156/510 (30.59%), Postives = 245/510 (48.04%), Query Frame = 0
Query:  107 EAIQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCDQLPDPEPVKDFSLDD---TAFICWSSGTTGTPKGICLSYQ--LVKHRVKTSLLDLPSPDLLT-TCMFHISG----IGKLLMGPLNQEAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFLVA--NSMDPHCLGSLAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTNAPLPKNAMGK 604
            E +  RI+  GN     D  T E     +L+ +  R A      G+K GD++ +   N L      L      AI +  + ++ + T    +  T  K+I C+   V++ L AIK+      IV+      V    D        +L  C+ + +    +   LDD   TA I  SSGTTG PKG+ LS    L+ H+   ++LD+ +  LL  + ++ I+G    +  ++ G L   AI + + +  ++IT   +    YK   +FL +    +      +     LSS+ I++  GA +   + + L  I P+++ L+ Y MTE+ F+    +S      G + E  Q+KI DP     LGP Q+GEI VK      GYY  PE T    D +G+ HTGD G+ DE G L++ GR+KE+IKYR   + P+E+EN++   P V E  + G P     E  +A V   PGA++TE+ +   VA N+ ++ +LR GV F +   P    GK
Sbjct:    9 EKLWIRIKSHGNKIAYLDACTEETITYAELQDKVVRCALWLQKQGIKSGDVISLCTGNHLNSIVPCLSAAYINAIFNPWNENMDLHTALHVLRLTTPKVIFCSEKAVDVILSAIKEEKCNPTIVIFGKHVDVISFFD--------ILRNCNDV-EVANFRYIELDDIKKTACILHSSGTTGMPKGVELSNYALLITHK---NILDVANMTLLWFSSLYWITGTILNLNMIMQGSL---AILYPEFN--EEITC--RLIEKYKVSFIFLSTSMINRFLKAGYVKKYF-LSSLKIILSSGAIIKPKVQEELRCILPHVDILHIYGMTEVSFITCQLSSHKNGSCGVIIENVQMKIIDPENGKILGPNQSGEIWVKSITMMNGYYRNPEATKHTIDEEGWLHTGDIGYIDEDGELFIIGRIKELIKYRGYQISPVEIENMLILHPAVLEAAIIGVPHALDDEHPLAYVIKRPGAKVTEQELIDFVARNMMDHYKLRAGVIFLDN-FPYTGSGK 497          
BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|746851087|ref|XP_011056052.1| (PREDICTED: 4-coumarate--CoA ligase 1-like isoform X2 [Acromyrmex echinatior])

HSP 1 Score: 202.986 bits (515), Expect = 3.655e-54
Identity = 149/503 (29.62%), Postives = 246/503 (48.91%), Query Frame = 0
Query:   99 KHMVEVYFEAIQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCD--QLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLS-YQLVKHRVKTSLLDLPSPDLLTTCMFHISGIGKLLMGPLNQ-EAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFLVANSMDPHCLGS---LAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTN 594
            K + E+ +  I++  +K+     CT+    E     +L+ +  R A      G+K GD++ I   N L      L T    AI +  + ++ + T    ++    K+I C+   V + L AIK+ +  A +VV     HV  +      + S +L  C+  ++ +   ++  ++  TA I  SSGTTG PKG+ LS Y ++   ++  +     P L  + ++ I+G+     G +   +AI + + D      LI K    YK   VFL ++   +L   S       LS +  VM  G  +   + + L  + P++  L CY MTE G ++      H  GS   L E  ++KI DP +   LG  Q+GE+ +K P    GYY  PE T    D++G+ H+GD G+ DE G L++  R+KE+IKY+  H+ P E+EN++   P V E  + G P     E  +A +   PG ++TE+ +   VA+N+++  +LRGGV F N
Sbjct:   25 KSLGELMWITIKSHEDKIAQLDACTE----ETVTYAELQNKVVRCALWLQKKGIKSGDVISICTGNHLNSIVPCLSTTYINAIFNPWNENMDLQTALYFLQLMMPKMIFCSEKSVNIILSAIKKQNYNATVVVF--GKHVDAV------SFSDILKNCNDVEVTNFRYIELDNIKKTACIMHSSGTTGIPKGVELSNYAMLFISLQYEINLTNVPSLWFSTIYWITGVIMSFSGIIQSTKAIIYPEFDEEMTCRLIQK----YKIETVFLSTNMMNRLHQ-SNCIQKYPLSFLKTVMFGGTSIRPKVQEELEHLLPHVQILQCYGMTETGGIITIQQPHHKNGSCGVLIENVKIKIVDPESGKVLGSNQSGEVWIKVPSLMNGYYRNPEATKNIIDNEGWLHSGDIGYVDEDGELFIIDRIKELIKYKGYHISPGEIENILLSHPAVLEAAIIGVPHILDDEHPLAYINKRPGVKVTEQELIDFVAKNMEDRCKLRGGVIFLN 510          
BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|746851085|ref|XP_011056051.1| (PREDICTED: 4-coumarate--CoA ligase 1-like isoform X1 [Acromyrmex echinatior])

HSP 1 Score: 202.986 bits (515), Expect = 3.766e-54
Identity = 149/503 (29.62%), Postives = 246/503 (48.91%), Query Frame = 0
Query:   99 KHMVEVYFEAIQARIEKVGNARYCTDGLTGEFNRIGDLEPQARRLAKLFTSLGVKRGDIVQISIPNDLEFSSLVLGTWVCGAIPSMTDPDLSVTTLQAQVEDTKAKIIICTSSKVEMFLKAIKQTSTGAQIVVLDDAAHVQEMKDAGLSTLSQLLAGCD--QLPDPEPVKDFSLDDTAFICWSSGTTGTPKGICLS-YQLVKHRVKTSLLDLPSPDLLTTCMFHISGIGKLLMGPLNQ-EAIFFKDSDLLKDITLILKACHDYKCGKVFLGSHHCAQLANLSEIPSGLDLSSVIIVMPIGARVPDAMLKRLNVIFPNMNFLNCYSMTELGFLVANSMDPHCLGS---LAEGAQVKIQDPVTQTALGPLQTGEILVKPPYPFPGYYNKPEETAKFCDSDGFCHTGDFGHYDEKGMLYLDGRMKEVIKYRNNHVYPMELENLIQDLPEVAEVGVYGKPDPASQELAVAVVRLVPGAQLTEEAIQKHVAENVDNYKQLRGGVRFTN 594
            K + E+ +  I++  +K+     CT+    E     +L+ +  R A      G+K GD++ I   N L      L T    AI +  + ++ + T    ++    K+I C+   V + L AIK+ +  A +VV     HV  +      + S +L  C+  ++ +   ++  ++  TA I  SSGTTG PKG+ LS Y ++   ++  +     P L  + ++ I+G+     G +   +AI + + D      LI K    YK   VFL ++   +L   S       LS +  VM  G  +   + + L  + P++  L CY MTE G ++      H  GS   L E  ++KI DP +   LG  Q+GE+ +K P    GYY  PE T    D++G+ H+GD G+ DE G L++  R+KE+IKY+  H+ P E+EN++   P V E  + G P     E  +A +   PG ++TE+ +   VA+N+++  +LRGGV F N
Sbjct:   27 KSLGELMWITIKSHEDKIAQLDACTE----ETVTYAELQNKVVRCALWLQKKGIKSGDVISICTGNHLNSIVPCLSTTYINAIFNPWNENMDLQTALYFLQLMMPKMIFCSEKSVNIILSAIKKQNYNATVVVF--GKHVDAV------SFSDILKNCNDVEVTNFRYIELDNIKKTACIMHSSGTTGIPKGVELSNYAMLFISLQYEINLTNVPSLWFSTIYWITGVIMSFSGIIQSTKAIIYPEFDEEMTCRLIQK----YKIETVFLSTNMMNRLHQ-SNCIQKYPLSFLKTVMFGGTSIRPKVQEELEHLLPHVQILQCYGMTETGGIITIQQPHHKNGSCGVLIENVKIKIVDPESGKVLGSNQSGEVWIKVPSLMNGYYRNPEATKNIIDNEGWLHSGDIGYVDEDGELFIIDRIKELIKYKGYHISPGEIENILLSHPAVLEAAIIGVPHILDDEHPLAYINKRPGVKVTEQELIDFVAKNMEDRCKLRGGVIFLN 512          
The following BLAST results are available for this feature:
BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides)
Total hits: 9
Match NameE-valueIdentityDescription
EMLSAG000000119031.166e-5828.93supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 ... [more]
EMLSAG000000104011.134e-4228.75supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 ... [more]
EMLSAG000000020811.186e-3224.38supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1... [more]
EMLSAG000000076961.817e-2826.08supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1... [more]
EMLSAG000000017091.512e-2234.10supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1... [more]
EMLSAG000000065773.993e-1421.13supercontig:LSalAtl2s:LSalAtl2s360:157972:160449:-... [more]
EMLSAG000000099042.271e-1122.74supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-... [more]
EMLSAG000000063095.497e-1129.69supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-... [more]
EMLSAG000000045075.229e-721.91supercontig:LSalAtl2s:LSalAtl2s235:163315:165883:-... [more]
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BLAST of 4-coumarate-- ligase-like vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
gi|75290242|sp|Q6ETN3.1|4CL3_ORYSJ4.628e-5030.19RecName: Full=Probable 4-coumarate--CoA ligase 3; ... [more]
gi|75311763|sp|Q9M0X9.1|4CLL7_ARATH2.687e-4728.04RecName: Full=4-coumarate--CoA ligase-like 7; AltN... [more]
gi|158564046|sp|Q84P21.2|4CLL5_ARATH3.068e-4728.83RecName: Full=4-coumarate--CoA ligase-like 5; AltN... [more]
gi|57012620|sp|Q9LU36.1|4CL4_ARATH1.300e-4528.80RecName: Full=4-coumarate--CoA ligase 4; Short=4CL... [more]
gi|148841518|sp|Q9S725.2|4CL2_ARATH5.209e-4529.88RecName: Full=4-coumarate--CoA ligase 2; Short=4CL... [more]
gi|1168218|sp|P41636.1|4CL_PINTA5.257e-4528.33RecName: Full=4-coumarate--CoA ligase; Short=4CL; ... [more]
gi|158564049|sp|Q84P25.2|4CLL2_ARATH5.351e-4528.88RecName: Full=4-coumarate--CoA ligase-like 2[more]
gi|12229631|sp|O24145.1|4CL1_TOBAC2.097e-4329.09RecName: Full=4-coumarate--CoA ligase 1; Short=4CL... [more]
gi|112800|sp|P14912.1|4CL1_PETCR2.190e-4329.28RecName: Full=4-coumarate--CoA ligase 1; Short=4CL... [more]
gi|75296548|sp|Q7XXL2.2|4CLL9_ORYSJ2.958e-4327.64RecName: Full=4-coumarate--CoA ligase-like 9[more]

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BLAST of 4-coumarate-- ligase-like vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR)
Total hits: 25
Match NameE-valueIdentityDescription
gi|1325285932|ref|XP_023327697.1|2.433e-6230.514-coumarate--CoA ligase-like [Eurytemora affinis][more]
gi|1325261085|ref|XP_023330441.1|3.225e-6029.534-coumarate--CoA ligase 1-like [Eurytemora affinis... [more]
gi|1325319021|ref|XP_023341279.1|2.102e-5928.444-coumarate--CoA ligase 1-like [Eurytemora affinis... [more]
gi|390364692|ref|XP_786981.3|3.857e-5830.89PREDICTED: 4-coumarate--CoA ligase 1 isoform X1 [S... [more]
gi|1325317676|ref|XP_023340582.1|1.195e-5627.154-coumarate--CoA ligase-like 7 [Eurytemora affinis... [more]
gi|1330905577|gb|PNF29545.1|6.727e-5630.60Luciferin 4-monooxygenase [Cryptotermes secundus] ... [more]
gi|909562920|ref|XP_013136653.1|1.854e-5531.55PREDICTED: 4-coumarate--CoA ligase 1-like [Papilio... [more]
gi|801398403|ref|XP_012059653.1|9.921e-5530.59PREDICTED: 4-coumarate--CoA ligase 1-like [Atta ce... [more]
gi|746851087|ref|XP_011056052.1|3.655e-5429.62PREDICTED: 4-coumarate--CoA ligase 1-like isoform ... [more]
gi|746851085|ref|XP_011056051.1|3.766e-5429.62PREDICTED: 4-coumarate--CoA ligase 1-like isoform ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
scaffold444_size168727supercontigscaffold444_size168727:161003..164796 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
maker2018-02-12 .496401
T. kinsejongensis vs L. Salmonis peptides2018-04-19
T. kingejongensis peptided Blastp vs. SwissProt2018-04-19
T. kingsejongensis proteins Blastp vs. NR2018-05-15
Properties
Property NameValue
Note-
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
snap_masked-scaffold444_size168727-processed-gene-0.25-mRNA-1snap_masked-scaffold444_size168727-processed-gene-0.25-mRNA-1Tigriopus kingsejongensismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at scaffold444_size168727:161003..164796-

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>snap_masked-scaffold444_size168727-processed-gene-0.25 ID=snap_masked-scaffold444_size168727-processed-gene-0.25|Name=4-coumarate-- ligase-like|organism=Tigriopus kingsejongensis|type=gene|length=3794bp|location=Sequence derived from alignment at scaffold444_size168727:161003..164796- (Tigriopus kingsejongensis)
ATGGGGGCGCGGGTTCCAATATTCGAAGTTCGAGACATCGAATTCCGCGA GACTACGAAGAAACTTAAGAAAGCCAATCATCTTCCAGACCGTGGGAAGT TGATCATTGGCCGGCTGAAATTGCCTAAGAAATGGGCTGCCTACGCTCGG CATCAAACTGTAATTGACGGCGTTTTGATAGTACAAACCCATGGTAAGGC CGTGATTAAGTAGATGCGTTCCATTACCAAACTTTTGCACTCGTACATAT GGCAAACCATTTCGGACGAACCAATAAACTGAAGTATCGTTCCCGATTCT GGTTGTAATGAAAAATCAACGTATTGGTAATGTATGCAATCGAGTCGGGA CAGCATCATCACCTACTAGTCTACGTACAATACTCTCCTTTTGTGTGACC TTGAGTGATCCGGTAGTCTTATGTTAATAAGTACCAAGAGCGCTCGGAAG TGTCGTTCCGACTTCGTTAGGAATACCACCAACATCAACACAACCCTCAT TTCTAGATTTAAGTTTTAGCAAGGCACTTGAGTTCGAGTTATTCGAATAA TGTCGCAATTATGTACTAGAATTAACGTACAATTGTGCTACAATAGCAGC CTAAGTCGGCGACAATGTTGAATGCTATTCTAAAGTATGCATATAAATAG GCCTAGATATCACACTATGGCCATCCTCCTAGCTATATACCTATGTACAT GTCGGTACTTTTTCCATCAAAGCACTCTTTGGTCTGAATTGATATACCTG ATGGACCGTTAAAACTGCTAGCTTCCATTAAGTACTCAAGTACTTAGCTT CCATTAGTAAATTATTGCAATATAGGTAATGTAAATTAGTAAATTTCTAG TCGCTCTAAGCTAGCAACTTTTGAGATCATTATTGCTCAGGGTATTCTGC GACCGATTTCGGTCAAATCTAACATCTCTATTATGAATATCAGATCACTT CGATGGGCTTCCGGTAGGTTCTTTTTCTTTAGTGCCCCTCTTTCACCTTG CCTAGTATTTAAAGCGGAGTCAGAAATACAAATGCATCCGTTCACTCTGC TAAATAAATGTTTAACGAGGTGTTTGATTAGTGGAAATAATAGCAAAAAT ATTCAAATAGTTGTTGCTTGAATTCCCCGCCCCGACAAGACGCTCGGAAA AAGCCATGTTCCAAACCTCATGTGTAGGTTGGGTTCTTGGAAATCCCAAG AGTCCCAACATTGAAATCTCCATGCACTGAATGTCTAAAATATGTCTTTC GCCAAGAGAATTGTACATATGAGACCATCCACACAAATACGCACATTCGT ACAAACACGACAAACAACTTAGCTGTAAATGGGAAAATCACAGTCCTTCA ACACCTTTGATCTACTGTAAACCTGGGACTGGTTCAGATGTAGACATTGG GACTTGAACTTGCAACTTTGTTCCCGTCTGTTGGATTTGAATCCGAGTTC TACAGTAATCCCGCCCGAACCTAGGACAATCGAGATTAACACATCGTGAG CAATCCCATCATTTTTTTGCCAGCCACACGATTTGGGGGAAAAACAGCTC GATGTGCCGTTCTATGGCTGGTATAAGTGGAGCACTTGCCGAACTAGACC ATGCGTAAGCACTTCAAGTTGGCTAGTTAACAATACAAATAACGTAGCCA TTGAATGTTGGTCAACTTCTTTTCCAAATATTCACCGTCTGAGAACAGAT TGGGAATTGCACGGAAAGGAGGTTAAGAAAAGTGTTGCAGACTCTTAAAG ATCTTTTGAAGCACGGCGGGCGGAGATTACGAAAGGGGCATTTGGATCGC ACTCCAGATAGCCTCGTATGTTGGTTTATCTAATTATGTACTATGCTCAT GTGCTTACGAGTAAAATAGCTGTACTGCACGTAATTTGACCAACACATGA TCTAAAAACTCAATGCTCGACCTCACACCTGCAAATCAATCTGACCAGAG AGGCCCAAACAGCACGTCAAGTTTGTACATATTGCCTCCGGCTGAATCTA ACTTTTGCATATTGAGGTGTATTTTATGCGTTTCGTTATCTCGTTTCTAC TGTGTTCGATTGCAATCATAAAAGAGGTGGACGAATCCGTGTCATCATCA CTCTCTGAGCATCCATTAAACATTCACGCACCAATCCAGAGGCAAATCTA AGCAAAATTATGTCGGTGATTGGGAGCAATAACATCCTCACCTCCCACGA CCCCCTGAACAACGCGGATAACCCTGTGACAGCCCCGGACAAGCACATGG TCGAGGTGTATTTCGAGGCTATTCAGGCCAGGATCGAGAAGGTGGGCAAC GCCAGGTACTGCACGGATGGGCTCACGGGCGAGTTCAACCGAATCGGCGA TTTGGAGCCCCAGGCCAGACGGTTGGCCAAGCTGTTCACCTCATTGGGAG TTAAACGGGGGGACATTGTCCAGATCTCCATCCCCAACGACTTGGAATTC TCCTCATTGGTCTTGGGAACCTGGGTGTGTGGAGCCATTCCATCGATGAC CGATCCAGACTTGTCGGTGACAACTTTGCAGGCCCAAGTCGAGGACACCA AGGCTAAGATTATCATCTGCACGTCATCCAAGGTTGAGATGTTCCTGAAG GCCATCAAGCAGACCAGTACTGGGGCCCAAATCGTGGTCTTAGATGACGC CGCGCACGTTCAGGAAATGAAGGATGCTGGCCTTTCCACCTTGAGTCAAC TCTTGGCTGGATGCGATCAGCTGCCTGACCCTGAACCAGTCAAGGATTTC AGTCTCGATGACACAGCCTTCATTTGTTGGTCGTCGGGCACGACCGGCAC TCCCAAGGGAATCTGCTTGTCCTACCAATTGGTGAAGCACCGAGTCAAGA CCTCCTTGCTCGACTTGCCCTCGCCTGACTTGCTCACCACGTGCATGTTT CACATCAGCGGCATTGGCAAGCTCCTCATGGGGCCGCTCAACCAAGAGGC GATTTTCTTCAAGGACTCAGACCTGCTCAAAGATATCACCCTGATCCTGA AGGCGTGCCACGACTACAAGTGTGGCAAGGTGTTCTTGGGATCGCATCAC TGCGCGCAATTGGCGAACTTGAGCGAGATCCCTTCGGGGCTCGACCTCAG CTCCGTGATTATCGTCATGCCCATCGGAGCCAGGGTCCCCGACGCCATGC TCAAGCGCCTCAATGTGATCTTCCCCAACATGAATTTTCTCAATTGCTAC TCAATGACCGAATTGGGTTTCCTAGTCGCCAACTCCATGGATCCGCATTG CTTGGGCAGTCTGGCCGAGGGTGCCCAAGTCAAAATCCAGGATCCAGTCA CCCAGACGGCTTTGGGACCGCTTCAGACGGGCGAGATCTTGGTCAAACCC CCGTATCCCTTTCCTGGGTACTATAACAAACCCGAGGAAACGGCCAAATT CTGCGACTCGGACGGGTTCTGCCACACTGGGGATTTCGGACACTACGATG AGAAAGGTATGCTCTACCTAGACGGTCGGATGAAAGAGGTCATCAAATAC CGGAACAACCACGTCTACCCGATGGAGTTGGAGAACCTGATCCAGGACCT CCCAGAGGTGGCTGAGGTGGGCGTGTATGGCAAACCCGATCCCGCCTCCC AGGAGTTGGCCGTAGCCGTGGTGCGTCTGGTACCTGGAGCCCAATTGACC GAGGAAGCCATCCAGAAGCATGTGGCCGAGAACGTGGATAACTACAAACA GCTTCGTGGTGGTGTTCGCTTCACCAATGCTCCTCTGCCCAAGAACGCGA TGGGTAAACCAGTGCGGAACCTTCTCGCCACCATAATGGGCTAA
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Synonyms
The feature '4-coumarate-- ligase-like' has the following synonyms
Synonym
Tk11374
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