glutamate synthase, snap_masked-scaffold455_size166772-processed-gene-0.25 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of glutamate synthase vs. L. salmonis genes
Match: EMLSAG00000004194 (supercontig:LSalAtl2s:LSalAtl2s221:469662:476711:-1 gene:EMLSAG00000004194 transcript:EMLSAT00000004194 description:"augustus_masked-LSalAtl2s221-processed-gene-4.2") HSP 1 Score: 945.266 bits (2442), Expect = 0.000e+0 Identity = 493/923 (53.41%), Postives = 628/923 (68.04%), Query Frame = 0
Query: 24 MPFDPDRPRYPEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTL--DLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIM-------------------------------VMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVTLGDMRKANKH---EIST--ERRPIIKSLALLNDH------SIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNALNLEDALDKICKEAEKAAREH--QFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLA-------FGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEAT-KYENKASYQKYVD 892
MPFD YP GLYDP+LEKDACGVGFIV+IDG SHD+ DA +LS RMEHRGACSADNDSGDGAGVL+AIP+++++K++ ++ +LP L YATGI+F + + K +FE A +L +L+V+ WR VPK+++ LG++A+ SEP + QVFV +E + E+ F ++LRK A+H + + KRFY+ SLS T +VYKGQFNPCQ++ YF DL+DP+F++H+ IVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGN+NLM+AREGVM + F LS L+P+VE D SDSG LDNV+EFLV G+RSLPEA+MTLVPEAWQND M KKNFY +AS EPWDGPALLTF+DGRYVGAILDRNGLRPSRYY+T +N M ASEVGV D DP +I++KGRL PG++LLVDT +K+I D +K IA SRPH + N+ + L +++A EIS+ RRPI N++ +I+ ++W+GDRR+P+FGY +ET++MLM+PMF TKKEALGSMGNDTPLACLS++QPLIYEYFKQLFAQVTNPPIDPFRE+IVMSLECPIGPEGN+L P + C RLWLKNPILS+ DLE LK+T++LGWK++VIDITY + ++L D + IC AE+A ++ Q +ILSDRN+G RIP+SA LASGAVHHHLI TRLRSKCAII+E+GE REVH +CV++GFG DAICPYMV EIA ML++E V ++ N+ AY Y A I K + S V L++ +V R G S G ++ ++ +KL GENE E+++ND A+ Q A+ ++ ASY + D
Sbjct: 1 MPFDSKSWTYPSAEGLYDPALEKDACGVGFIVNIDGRTSHDIFEDAAELSRRMEHRGACSADNDSGDGAGVLIAIPKDYYQKKLSEVNVQLPRL--YATGIIFCDESDESDVKKDFEKHAESLKGNLRVIHWREVPKDSNSLGSVARASEPKLLQVFVTSEEEGI--ENKCFDKYIYILRKTATHYIPACK-----DSRKRFYICSLSRTAIVYKGQFNPCQIWNYFTDLMDPEFESHLLIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNVNLMEAREGVMVSPDF-EDLSTLYPIVEPDMSDSGCLDNVVEFLVHVGDRSLPEAMMTLVPEAWQNDDQMPSNKKNFYHWASFYMEPWDGPALLTFTDGRYVGAILDRNGLRPSRYYITKNNRMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASEVGVIDIDPTQIIKKGRLQPGKILLVDTDVQKVIDDHELKSSIAXSRPHGDFLNKSIDLKIIKEAGSEYAAEISSLPRRRPINLHENDFNNNFDYGEENILEKLWRGDRRLPIFGYNIETLEMLMLPMFKTKKEALGSMGNDTPLACLSEMQPLIYEYFKQLFAQVTNPPIDPFREKIVMSLECPIGPEGNILNPGD--CTRLWLKNPILSVNDLEHLKRTSILGWKSRVIDITYLSDDQISLSDIVSNICNRAEEAVKKEGVQLLILSDRNVGVNRIPVSAALASGAVHHHLIATRLRSKCAIIVESGEAREVHHLCVLIGFGADAICPYMVLEIAAMLKDEGVEATKYENKNAYQKYVDATMETIKKCSIRGQFEIDFSKGTPVDVSEVELASNIVKRFVTGAMS-FGSISAEAHGTLAVAMNKLGGKSNTGEGGENE------------------ERYLNDIRRSAIKQVASGRFGVSASYLAHAD 892 HSP 2 Score: 780.015 bits (2013), Expect = 0.000e+0 Identity = 402/654 (61.47%), Postives = 474/654 (72.48%), Query Frame = 0
Query: 1481 GCTGRNFAAGMSGGIAYVLDHEGN--FKDKCNKEMVDLL--PIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGD--TLLPGLGGVIHP 2128
G GRNFAAGMSGGIAYV+ +GN F+ KC EMV+L+ ++DED FL + LQ+FV+ TGS VA IL + + F+KV+P EY+ A+ +++ S +PP N+ EE + DIED + + ++ LDKT+GF+KY R YRD E R KDW EIYDF VRK LK QAARCMDCGVPFCHS +HGCPLGN+IP+FNDLVFHD WKEAL LLQTNNFPEFTGRVCPAPCEG+CVLGIN P VTIKNIECAIID AFE GWIK PP K KT+AI+GSGP+GLAAADQLNKAGH VTVYERNNRIGGLLQYGIP+MKLSK++VQRRVDLM +EGI FK N + + + ++K DA+L G+T PRD+PIP R++ GIHFAMEFLQ QQ GD ++ I A D DVIVIGGGDTG DCI +SLRQGAKSIT+FEILP P + R DNPWPQFPR+FKVDYGH+EV VK G DPRRY+T S EFL DE +++GIRTV V W KDD GRWV V SEK+Y QL LL MGFLGP+ V+ +E DPR NI TK+ YA G+FAAGDCRRGQSLVVWAITEGRQAAR++DE+L+G + LP +GGVI P
Sbjct: 1210 GSGGRNFAAGMSGGIAYVV-KKGNETFETKCXXEMVELVEPKSMEDEDKSFLLKTLQDFVEATGSEVAAAILS--GNPFESFIKVYPMEYRXAIENKRRGSSVEKQKEATKPP-------------LNEHEEEIIKDIED------LRIEPKKSALDKTKGFMKYPRVYDNYRDPESRTKDWDEIYDFPKVRKSLKVQAARCMDCGVPFCHSNNHGCPLGNVIPRFNDLVFHDQWKEALKTLLQTNNFPEFTGRVCPAPCEGSCVLGINSPPVTIKNIECAIIDKAFENGWIKAQPPLKX--KTIAIIGSGPSGLAAADQLNKAGHNVTVYERNNRIGGLLQYGIPSMKLSKEIVQRRVDLMTEEGINFKVNSTINES-NILELKKEYDALLFCTGSTIPRDIPIPGRKSTGIHFAMEFLQKLQQKNLGD--EVKTIDAKDLDVIVIGGGDTGNDCIASSLRQGAKSITTFEILPQPTELRGEDNPWPQFPRIFKVDYGHQEVIVKNGN-DPRRYSTMSKEFLSDENGRISGIRTVCVSWEKDDKGRWVMSEVPNSEKLYPAQLVLLCMGFLGPDKKVLDYLNIELDPRGNIQTKD--YAVGN-GIFAAGDCRRGQSLVVWAITEGRQAARKLDEYLIGSGVSQLPIVGGVIVP 1832 HSP 3 Score: 427.557 bits (1098), Expect = 5.095e-122 Identity = 262/527 (49.72%), Postives = 319/527 (60.53%), Query Frame = 0
Query: 877 EATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVA-------ALLGADEFGFSTAPLIAMGSTDSP---KVDLLNFEKILTNASLLR-PGASILGGSVEQDFQLEARLDAEIIPICQDALDGKP-GTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLE 1391
EATKYENK +YQKYVDA +E+I+ C+IRGQ EI+F K +D+ EVE AS IVKRF TGAMSFGSIS EAH TLAVAMNKLGGKSNTGEGGEN ERYLN RSAIKQVASGRFGV+ASYLA+ADELQIKMAQGAKPGEGGELPG+KVT++IAKTR SVP ARISVKLVSEVGVGV+A+GVAKGK+EHILISGHDGGTGASSWTGIKGAGLPWELG++ETHQ L MNNLRSR + Q L+ G V+ +G I G D +++ + G + + L+ + + R + + + L+A A LG +F + T++P K LLNF +L +A +R P GGS+ QDFQL RLD E+I C ++ I +E I N QR FGATLS+ IA +Y++SGLP I+I L GS G++F + GI ++
Sbjct: 768 EATKYENKNAYQKYVDATMETIKKCSIRGQFEIDF--SKGTPVDVSEVELASNIVKRFVTGAMSFGSISAEAHGTLAVAMNKLGGKSNTGEGGENEERYLNDI----RRSAIKQVASGRFGVSASYLAHADELQIKMAQGAKPGEGGELPGYKVTVEIAKTRRSVP--------------------------------ARISVKLVSEVGVGVIAAGVAKGKAEHILISGHDGGTGASSWTGIKGAGLPWELGIAETHQTLVMNNLRSRVVLQTDGQLR---------------TGFDVMVAG----------ILGADEFGFSTAPLIVLGCTMMRKCHLNTCPVGIATQDPILRKKFEGQPEHVVNYFFLIAEEIREFMAKLGCTKFNDLIGHTEFLKVTENPIPSKTHLLNFNSLLHSAESMRIPKKH--GGSIPQDFQLNLRLDNEVIKACDTIINSDSLNPISLEYPIVNTQRTFGATLSYHIAMKYNESGLPRDNIINISLHGSGGRNFAAGMSGGIAYVVK 1229
BLAST of glutamate synthase vs. L. salmonis genes
Match: EMLSAG00000001514 (supercontig:LSalAtl2s:LSalAtl2s125:349817:362791:1 gene:EMLSAG00000001514 transcript:EMLSAT00000001514 description:"maker-LSalAtl2s125-augustus-gene-3.14") HSP 1 Score: 73.1738 bits (178), Expect = 6.620e-13 Identity = 71/308 (23.05%), Postives = 132/308 (42.86%), Query Frame = 0
Query: 1656 KDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAP--CEGACVLGINEP-AVTIKNIECAIIDNAFEEG--------WIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHL-VTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNR--EAEGIHFAMEFL-QTWQQNQHGDKMDLS----KITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSI 1944
KD+ +I + +++RC+ C C + CP + F + N+ A +L N G VCP C G C L +E A+ I ++ ++ F++ +I + P + K +A++G GPA ++ A L + G+ +T++E++ +GGL +P +L D+ N +A+ + +G P+ +PI + E+ G +FL + + +++G S ++ + DVIV+G GDT DC +++R GAK +
Sbjct: 64 KDFNDIKHTSLSERAALLESSRCLKCADAPCQKS---CPTQLDVKSFIGSISTKNYYGAAKAILSDNPLGLTCGMVCPTSDLCVGGCNLAASEEGAINIGGLQQFAVE-MFKKAKIPQVLPPYISKNRPDSYNSK-IALIGCGPASISCATFLGRLGYRDITIFEKDEFVGGLNSSELPAFRLPYDM--------------------------------NYEAIFVGIGNPEPKKIPIFEKLTESNGFFTXKDFLPKVAKASKNGMVSKCSGCVPQLPELFGDVIVLGAGDTAFDCATSAIRCGAKRV 334
BLAST of glutamate synthase vs. SwissProt
Match: gi|417073|sp|Q03460.1|GLSN_MEDSA (RecName: Full=Glutamate synthase [NADH], amyloplastic; AltName: Full=NADH-GOGAT; Flags: Precursor) HSP 1 Score: 1976.83 bits (5120), Expect = 0.000e+0 Identity = 1067/2133 (50.02%), Postives = 1402/2133 (65.73%), Query Frame = 0
Query: 28 PDRPRYPEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLGPGN--LQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVV-RKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFL-DLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFG---SQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWW-NQHVTLGDMRKANKHEISTERRPIIKSLALLNDHSIVARIWQGDRRMPM--FGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNALN-LEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVI----SSDI-TNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAF-----GENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERY--LNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLG--ADEFGFSTAPLIAMGSTDSPKVD--LLNFEKILTN---ASLLRPGASILGGSVE-----QDFQLEARLDAEIIPICQDALD-GKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVA-KKQEVILD--KTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSK-DVVQRRVDLMAQEGIIFKANVDVGKD--ITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKM-DLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGD 2116
PD+P GLYDP+ +KD+CGVGF+ ++G S + DA ++ RM HRGAC + ++GDGAG+LVA+P F+++ V +LPP G YA G+ FL + +ESK+ F +A +L KVL WR VP +N LG A+ +EP++EQVF+ + V E M++ + + S G+ FY+ SLSS T++YKGQ P QL +Y+ DL + F +++ ++H+RFSTNTFPSW+RA P RVL HNGEINTLRGN+N +KAREG++ K+ G + L + P+V+ ++SDSG D VLEFL+ +G +SLPEAVM ++PEAWQND MD +K FY + S EPWDGPAL++F+DG Y+GA LDRNGLRP R+YVT ++MASEVGV D P + +KGRL PG MLLVD ++ ++ D+ +K++ +L+RP+ W Q + L D+ + HE I L ND + + P+ FGY++E++++L++PM EALGSMGNDTPLA +S + L +EYFKQ+FAQVTNPPIDP RE+IV S+ C +GPEG+L E E C RL LK P+LS ++E +KK GW++KVIDITY LE+ALD+IC EA A E + ++LSDR + + +S+ LA GAVH HL+ T R++ A+++E+ E REVH C +VGFG DAICPY+ E L+ + I S D + + Y A G+ KV+AKMGIST+ SYK AQIFEA+GLS+EV+++CF GT SR+ G TF +LA+++L H+LAF ++ L +PG H+R GGE H+NDP ++A LQEA + + +Y++Y E + C +RG L+ +D + + I EVEPASEIVKRF TGAMS+GSIS EAH+ LA AMN +GGKSNTGEGGE P R L RSAIKQVASGRFGV++ YL NADELQIKMAQGAKPGEGGELPG KV DIA TR+S GVGLISPPPHHDIYSIEDLAQLI+DLK NP ARISVKLVSE GVGV+ASGV KG +EH+LISGHDGGTGAS WTGIK AGLPWELGL+ETHQ L N+LR R Q LK T R +V + + G S LI+ G P +G++ VL R + + F +VA + + GF T + +G +D +VD ++ L N + LLRP A + + + QD L+ LD ++I + AL+ G P + IE I N RA G LSHE+ KRY+ +GLP I IQ GSAGQSF FL GI + LEGD+NDY+GKGLSGG+V+++PPK S+ F ++NI+ GNV LYGAT G+A+ G+AAERFCVRNSGALA+VEGVGDHGCEYMTGG V++LG TGRNFAAGMSGGIAYVLD +G F+ +CN E+VDL + ++EDII L+ +Q+ T S++A +L ++ + FVKVFPREY+R L K S+ S +++E ++++ + V D E ++ +K A K+ + D K RGFV Y+RE YRD R DW E+ LK Q+ARCMDCG PFCH + GCPLGN IP+FN+LV+ + W+EAL++LL+TNNFPEFTGRVCPAPCEG+CVLGI E V+IKNIECAIID AFEEGW+ P PP KR+GK VAIVGSGP+GLAAADQLNK GH+VTV+ER +RIGGL+ YG+P MK K D+VQRRV+LMA+EGI F N ++G D +++ ++ NDA++L VGAT PRDLP+P RE G+HFAMEFL ++ + D + I+A K V+VIGGGDTG DCIGTS+R G ++ + E+LP PP TR+ NPWPQ+PR+F+VDYGH+E K+GK DPR Y + F+ DE + G+ V+V W KD+ G++ + +EGSE+I E L LLAMGFLGPE + + G+E+D RSN G ++T GVFAAGDCRRGQSLVVWAI+EGRQAA +VD +L +
Sbjct: 86 PDKP-----MGLYDPAFDKDSCGVGFVAELNGQSSRKTVTDALEMLVRMTHRGACGCEANTGDGAGILVALPHGFYQEVVD---FQLPPQGNYAVGMFFLPKSDSRRKESKNIFTKVAESLGHKVLGWRSVPTDNTGLGKSAQLTEPVIEQVFLTPSSDSKVDLEKQMYILRKLSMVSITSALNLQSDGI------TDFYICSLSSRTVIYKGQLTPAQLGEYYYADLGNERFTSYMALIHSRFSTNTFPSWDRAQPFRVLGHNGEINTLRGNVNWIKAREGLLKCKELGLSENDLKKFLPIVDANSSDSGCFDGVLEFLLHSG-KSLPEAVMMMIPEAWQNDKNMDPQRKAFYEYYSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTHSGRVIMASEVGVVDIPPEDVCRKGRLNPGMMLLVDFEKQIVVNDDALKEQYSLARPYGDWLEKQKIELKDIIDS-VHESDIVPPTISGVPPLSNDDVDMENMGIQGLLAPLKAFGYSVESLEILLLPMAKDGVEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMRCMVGPEGDLTETTEEQCHRLSLKGPLLSTKEMEAIKKMNYRGWRSKVIDITYSKERGTKGLEEALDRICTEAHNAISEGYTTLVLSDRAFSKKHVAVSSLLAVGAVHQHLVKTLERTRVALMVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPPKASGDFNSKDELVKKYFKASTYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLAQDALHLHELAFPSRIFSPGSAEAVALPNPGDYHWRKGGEVHLNDPLAIAKLQEAARTNSVDAYKQYSKTIHELNKACNLRGLLKF---KDAASKVPISEVEPASEIVKRFCTGAMSYGSISLEAHTALATAMNTIGGKSNTGEGGEQPSRMEPLADGSRNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARISVKLVSEAGVGVIASGVVKGHAEHVLISGHDGGTGASRWTGIKSAGLPWELGLAETHQTLVANDLRGRTTLQTDGQLK----TGR-------DVAIAALLGAEEYGFSTAPLIT-----LGCIMMRKCHKNTCP--VGIATQDPVL-------REKFAGEPEHVINFFFMVAEEMREIMSQLGFRTVNEM-VGRSDMLEVDKEVVKGNAKLENIDLSLLLRPAAELRPEAAQYCVQKQDHGLDMALDNKLISLSNAALEKGLP--VYIETPICNTNRAVGTMLSHEVTKRYNLAGLPADT-IHIQFTGSAGQSFGAFLCPGITLELEGDSNDYIGKGLSGGKVVVYPPKGSN--FDPKDNILIGNVALYGATRGEAYFNGMAAERFCVRNSGALAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGTFQSRCNLELVDLDKVEEEEDIITLRMLIQQHQRHTNSLLAKEVLVDFENLLPKFVKVFPREYKRVLASMK--SDAASKDAVERAAEDVDEQDDEAQAVEKDAFEELKKLATASLNEKPSEAPKRPSQVTDAVKHRGFVAYEREGVQYRDPNVRLNDWNEVMMETKPGPLLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALERLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMIPRPPVKRTGKRVAIVGSGPSGLAAADQLNKMGHIVTVFERADRIGGLMMYGVPNMKTDKVDIVQRRVNLMAEEGINFVVNANIGLDPLYSLERLREENDAIVLAVGATKPRDLPVPGRELSGVHFAMEFLHANTKSLLDSNLQDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTAVVNLELLPQPPPTRAPGNPWPQWPRIFRVDYGHQEAETKFGK-DPRTYEVLTKRFVGDENGVVKGLEVVRVCWEKDETGKFQFKEIEGSEEIIEADLVLLAMGFLGPEATIAEKLGVERDNRSNFKADYGRFSTSVDGVFAAGDCRRGQSLVVWAISEGRQAAAQVDSYLTNE 2165
BLAST of glutamate synthase vs. SwissProt
Match: gi|300680979|sp|Q0DG35.2|GLT2_ORYSJ (RecName: Full=Glutamate synthase 2 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 2; Short=NADH-GOGAT 2; Flags: Precursor) HSP 1 Score: 1975.29 bits (5116), Expect = 0.000e+0 Identity = 1067/2132 (50.05%), Postives = 1383/2132 (64.87%), Query Frame = 0
Query: 32 RYPEKA-GLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLGPGNLQESKDE--FEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFL-DLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFG---SQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWN-QHVTLGDMRKANKHEISTERRPIIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNA-LNLEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSD----ITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGEN-----ECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLN-QDPDYNMR-SAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVG---VVASGVAKGKSEHILISGHDGGTGASSWTGI---KGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVE-----QDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRAL-----LDQKAASEQTSSNSIEEPPKEI----NGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHS--TSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKD-VVQRRVDLMAQEGIIFKANVDVGKD--ITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKM-DLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGD 2116
+ PE + GLYDPS+E+D+CGVGFI + G S + DA ++ +RM HRGAC + ++GDGAG+LVA+P FF + G LPP G+YA G+ F+ + + K + F A L VL WRRVP +N LG A +EP++EQVFV + K D F +VLR+ + + GV G K FY+ SLSS T+VYKGQ P QL YF DL D F +++ ++H+RFSTNTFPSW+RA P RVL HNGEINTLRGN N MKAREG++ + G ++ +L P+V+ +SDSGA+DNVLE L+ +G RS PEAVM ++PEAWQND MD +K Y F S EPWDGPAL++F+DGRY+GA LDRNGLRP R+YVT ++MASEVGV D P + +KGRL PG MLLVD ++ D+ +KKE + RP+ W Q + L D+ ++ +E I +L + ++ I + FGYT E ++MLM+PM +EALGSMGNDTPLA +S + L +EYFKQ+FAQVTNPPIDP RE+IV S+EC IGPEG+L E ER C RL LK+P+L+ ++E +KK GW++KV+DITY N + L+ LDKIC +A +A E + ++LSDR R+ +S+ LA GAVH HL++ R++ +++E+ E REVH ++GFG DAICPY+ E L+ + I + T E Y A + G+ KV+AKMGIST+ SYK AQIFEA+GL++EVV +CF GT SR+ G TF +LA+++L H++AF D+ L +PG H+R GE H+NDP S+A LQEA + ++ +Y++Y E + CT+RG L+ + I +DEVEPA EIVKRF TGAMS+GSIS EAH++LA AMN LGGKSNTGEGGE P R + D N R SAIKQVASGRFGV+ YL NA E+QIKMAQGAKPGEGGELPG KV DIA TR+S GVGLISPPPHHDIYSIEDLAQLI+DLK NP ARISVKLVSE GVG+VASGV KG ++H+LISGHDGGTGAS WTGIK AGLPWELGL+ETHQ L N LR R + Q +K A + E G ++ G + H + G ++ + K AG P V+ + + V + Q+ GF V ++G + P + G+ +DL + LL+P A I G+V+ QD L+ LD ++I AL K + IE + N RA G LSHE+ KRY GLP I I+L GSAGQSF FL GI + LEGD+NDYVGKGLSGG+++++PP+NS F ++NI+ GNV LYGAT G+A+ G+AAERFCVRNSGA A+VEG+GDHGCEYMTGG +ILG TGRNFAAGMSGGIAYV D +G F +CN E+VDL +++++DI L+ +Q+ T S +A +IL N+ + F+KV+PR+Y+R L +EQ + +++ P E+ NG +V N++ S + + +AV K RGF+KY+RE YRD +R KDW E+ LK Q+ARCMDCG PFCH + GCPLGN IP+FN+LV + W EALD+LL+TNNFPEFTGRVCPAPCEG+CVLGI + V+IK+IECAIID FEEGW+ P PP +R+GK VAIVGSGPAGLAAADQLNK GH VTV+ER +RIGGL+ YG+P MK K+ +VQRRV+LMA+EG+ F N VG D +V+ +++ NDA++L GAT P+DLPIP RE GIHFAMEFL ++ + D + I+A + V+VIGGGDTG DCIGTS+R G ++ + E+LP PP R+ DNPWPQ+PR+F+VDYGH+E + K+GK DPR Y + F+ DE + + ++VEW K + GR+ + VEGSE+I E L LLAMGFLGPE V GLEQD RSN + G++AT GVFAAGDCRRGQSLVVWAITEGRQAA VD +L D
Sbjct: 89 KIPESSIGLYDPSMERDSCGVGFIAELSGEYSRKTVDDAIEMLDRMAHRGACGCEKNTGDGAGILVALPHNFFREVTKDAGFELPPPGEYAVGMFFMPTDDKRREKSKLLFREKAELLGHTVLGWRRVPTDNSGLGQSAVDTEPVIEQVFVTKSASS--KAD--FERQMYVLRRFSVMSIREVLGVKNG-GTKDFYMCSLSSRTIVYKGQLKPSQLKGYFFADLGDESFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNKNWMKAREGLLKCEGLGLTRDEMLKLLPIVDATSSDSGAIDNVLELLIQSG-RSAPEAVMMMIPEAWQNDVNMDPERKALYEFFSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTYSGRVIMASEVGVVDVPPQDVSRKGRLNPGMMLLVDFENHCVVNDDELKKEYSKVRPYGEWLKRQRIQLTDIIES-VNEAERIAPSISGALPITKENKADMGICGILTPLKAFGYTREALEMLMLPMAKDGQEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLSETTERQCHRLTLKSPLLNTNEMEAIKKMNYRGWRSKVLDITYPKKNGRMGLKQTLDKICAQAREAIHEGYTILVLSDRGFSSERVAVSSLLAVGAVHQHLVSHLERTRIGLLVESAEPREVHHFSTLIGFGADAICPYLAIEAIWRLQIDGRIPPNDGKPYTQEQLIEKYFYASNYGMMKVLAKMGISTLASYKGAQIFEALGLASEVVSKCFEGTPSRVEGATFEMLAQDALRLHEIAFPSRTLPPGSADANALPNPGDYHWRKNGEVHLNDPFSIAKLQEAARINSREAYKEYSRRIYELNKACTLRGMLKF---REIPNQISLDEVEPAKEIVKRFCTGAMSYGSISLEAHTSLAEAMNTLGGKSNTGEGGEQPCRMVPLPDGSKNPRISAIKQVASGRFGVSIYYLTNAVEVQIKMAQGAKPGEGGELPGHKVIGDIAVTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGIVASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANGLRGRAVLQTDGQMKTGRDVAVACLLGAEEFGFSTAPLITLGCIMMRKCHT----NTCPAGIATQDPVLRAKFAGKP--------EHVINYFFMLAEEVREIMAQL--GFRT-VNEMVGRSDM-LEIDPKVLEGNEKLENIDL---------SRLLKPAAEISPGAVQYCVEKQDHGLDMALDNKLIASSTAALR-KGVRVFIETPVRNINRAVGTMLSHEVTKRYHIHGLPSDT-IHIKLNGSAGQSFGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRNSR--FNPQDNIVIGNVALYGATKGEAYFNGMAAERFCVRNSGAQAVVEGIGDHGCEYMTGGTAVILGKTGRNFAAGMSGGIAYVYDVDGKFSSRCNYELVDLYAVVEEDDITTLRMMIQQHRLHTQSDLARDILLNFDTLLPKFIKVYPRDYKRVLDKLKEEKAAKEAEQKAREVVDKKPVEVIQAPNGISVKTEKVMNEEPSSRPSRVSNAV---------------KYRGFIKYEREGTSYRDPNERVKDWNEVAIELVPGPLLKTQSARCMDCGTPFCHQEGSGAGCPLGNKIPEFNELVHQNRWHEALDRLLETNNFPEFTGRVCPAPCEGSCVLGIIDNPVSIKSIECAIIDKGFEEGWMVPRPPLRRTGKRVAIVGSGPAGLAAADQLNKMGHFVTVFERADRIGGLMMYGVPNMKADKEGIVQRRVELMAKEGVQFIVNAHVGSDPLYSVEKLRSENDAIILACGATKPKDLPIPGRELAGIHFAMEFLHANTKSLLDSNLEDGNYISAQGRKVVVIGGGDTGTDCIGTSIRHGCTNLVNLELLPEPPRKRAPDNPWPQWPRIFRVDYGHQEATSKFGK-DPRSYKVLTKRFIGDENGNVKALEVIRVEWGKVN-GRFQFKEVEGSEEIIEADLVLLAMGFLGPEATVANKLGLEQDMRSNFKAQFGNFATNVEGVFAAGDCRRGQSLVVWAITEGRQAAAAVDNYLSKD 2164
BLAST of glutamate synthase vs. SwissProt
Match: gi|122222486|sp|Q0JKD0.1|GLT1_ORYSJ (RecName: Full=Glutamate synthase 1 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 1; Short=NADH-GOGAT 1; Flags: Precursor) HSP 1 Score: 1962.58 bits (5083), Expect = 0.000e+0 Identity = 1072/2152 (49.81%), Postives = 1395/2152 (64.82%), Query Frame = 0
Query: 27 DPDRP--RYPEKA-GLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFL--GPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLF-QYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFG---SQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWN-QHVTLGDMRKANKHEISTERRP--IIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITY-EVSNALNLEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSD-----ITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGEN-----ECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERY--LNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLG--ADEFGFSTAPLIAMGSTDSPKVD--LLNFEKILTNASL---LRPGASILGGSVE-----QDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCH--STSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSK-DVVQRRVDLMAQEGIIFKANVDVGKD--ITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKM-DLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFL------VGDTLLPGLGGVIHP 2128
+P RP PE + GLYDPS E+D+CGVGF+ + G + DA ++ RM HRGAC + ++GDGAG+LVA+P FF + G LP G+YA G++FL + SK EF+ +A +L +L WRRVP +N LG A ++EP++EQVF+ + + F ++LR+++ + + +G + FY+ SLSS T+VYKGQ PCQL Y+ DL +F +++ +VH+RFSTNTFPSW+RA P RVL HNGEINTL+GN N MKAREG++ ++ G Q S++ P+V+ +SDSGA D VLE L+ G RSLPEAVM ++PEAWQND M+ KK Y F S EPWDGPAL++F+DGRY+GA LDRNGLRP R+YVT +VM SEVGV D +L+KGRL PG MLLVD ++ DE +K + + + P+ W Q + L D+ ++ TER I SL N+ A + + FGYT+E ++ML++PM EALGSMGNDTPLA +S + L +EYFKQ+FAQVTNPPIDP RE+IV S+EC IGPEG+LLE E+ C RL L+ P++S+ ++E +KK GW++KV+DITY + S LE+ LD+IC EA A ++ + ++LSDR R+ +S+ LA GAVH HL+ R++ +++E+ E REVH C +VGFG DA+CPY+ E L+ + I + + E Y A + G+ KV+AKMGIST+ SYK AQIFEA+GLS+EV+ +CF+GT SRI G TF +LA ++L H+LAF D++ L +PG H+R GE H+NDP ++A LQEA + ++A+Y++Y E + C +RG L+ +D + I +DEVEPASEIVKRF TGAMS+GSIS EAH+ LA+AMNKLGGKSNTGEGGE P R L RSAIKQVASGRFGV++ YL NADELQIKMAQGAKPGEGGELPG KV DIA TRHS GVGLISPPPHHDIYSIEDLAQLI+DLK +NP ARISVKLVSE GVGVVASGV KG ++H+LISGHDGGTGAS WTGIK AGLPWELGL+ETHQ L N LR R + Q LK +V V + G S LI+ G P +G++ VL R + + F ++A L + GF T + +G +D +VD ++ + L N L L+P A I G+ + QD L+ LD ++I + + AL+ K + IE I N RA G LSHE+ KRY GLP G I ++L GSAGQS FL GI + LEGD+NDYVGKGLSGG+++++PP++S F E+NI+ GNV LYGAT G+A+ G+AAERFCVRNSGA A+VEG+GDHGCEYMTGG V+ILG TGRNFAAGMSGGIAYV D +G F +CN E+VDL + ++EDI LK +++ TGS+VA +IL N+ + FVKVFPR+Y+R L + KA + ++ + VT K + E + V+ + K RGF+ Y+RE YRD +R KDW+E+ L Q+ARCMDCG PFCH S+ GCPLGN IP+FN+LV + W+EALD+LL+TNNFPEFTGRVCPAPCEG+CVLGI E V+IK+IECAIID FEEGW+ P PP +R+GK VAI+GSGPAGLAAADQLNK GH VTV+ER +RIGGL+ YG+P MK K ++VQRRV+LMA+EGI F N +VG D +++ +++ NDAV+L GAT PRDL IP RE G+HFAMEFL ++ + D I+A K V+VIGGGDTG DCIGTS+R G SI + E+L PP R+ DNPWPQ+PR+F+VDYGH+E S K+G DPR Y + F+ DE + + V+V+W K D GR+ + +EGS + E L LLAMGFLGPE + + GLE+D RSN + G++AT G+FAAGDCRRGQSLVVWAITEGRQAA VD++L + + P G + P
Sbjct: 75 EPVRPLSTLPESSIGLYDPSRERDSCGVGFVAELSGDYKRATVNDALEMLERMAHRGACGCEKNTGDGAGILVALPHNFFREVTKDAGFELPQPGEYAVGMVFLPIDEKRRERSKAEFQKVAESLGHVILGWRRVPTDNSDLGESALQTEPVIEQVFLTKSS----SSEADFEQQLYILRRLSILSIRAALNLRRG-GKRDFYMCSLSSRTIVYKGQLKPCQLKGYYYADLGHENFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLKGNKNWMKAREGLLECEKLGLTKDQFSKILPIVDATSSDSGAFDGVLELLIRGG-RSLPEAVMMMIPEAWQNDVNMEPEKKALYEFLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVVMGSEVGVVDVPSKDVLRKGRLNPGMMLLVDFENHTVVDDEALKAQYSKAHPYGEWLKRQKIYLKDIVESVPE---TERVAPGISGSLTQKNEKKEHAGVNGIVTPLKAFGYTVEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLLETTEKQCNRLALEGPLVSIDEMEAIKKMNYRGWRSKVLDITYPKKSGRKGLEETLDRICTEARGAIKKGYTVLVLSDRGFSSDRVAVSSLLAVGAVHQHLVANLERTRVGLLVESAEPREVHHFCTLVGFGADAVCPYLAIEAIWCLQNDGKIPPNGDGKPYSKEELVKKYFYASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIRKCFDGTPSRIEGATFEMLARDALRLHELAFPSRAPPPGSADAKALPNPGDYHWRKNGEVHLNDPLAMAKLQEAARVNSRAAYKEYSRRIQELNKTCNLRGMLKF---KDTADMISVDEVEPASEIVKRFVTGAMSYGSISLEAHTALAMAMNKLGGKSNTGEGGEQPSRMEPLANGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAVTRHSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNPRARISVKLVSEAGVGVVASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANGLRGRAILQTDGQLKTGK-----------DVAVACLLGAEEFGFSTAPLIT-----LGCIMMRKCHTNTCP--VGIATQDPVL-------REKFAGEPEHVINFFFMLAEELREIMSQLGFRTITEM-VGRSDMLEVDPEVVKSNEKLENIDLSLILKPAAEIRPGAAQYCVEKQDHGLDMALDNKLIALSKAALE-KEVRVFIETPIQNTNRAVGTMLSHEVTKRYHMKGLPAGT-IHVKLTGSAGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRDS--TFIPEDNIVIGNVALYGATIGEAYFNGMAAERFCVRNSGAQAVVEGIGDHGCEYMTGGTVVILGKTGRNFAAGMSGGIAYVYDIDGKFSVRCNHELVDLYHVEEEEDITTLKMMIEQHRLNTGSVVARDILSNFDTLLPKFVKVFPRDYKRVLDNMKAEKAAAKLAKEPK--------ISNGVSVTTKKVQP-----EQSTNRPTRVSNAK-----KYRGFISYERESISYRDPNERVKDWKEVAIESVPGPLLNTQSARCMDCGTPFCHQESSGAGCPLGNKIPEFNELVHQNRWREALDRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKGFEEGWMVPRPPLQRTGKKVAIIGSGPAGLAAADQLNKMGHFVTVFERADRIGGLMMYGVPNMKTDKIEIVQRRVNLMAEEGITFVVNANVGSDPLYSIERLRSENDAVILACGATKPRDLGIPGRELSGVHFAMEFLHANTKSLLDSNLEDGRYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTSIVNLELLTKPPSKRAADNPWPQWPRIFRVDYGHQEASSKFGN-DPRTYEVLTKRFIGDENGNVKALEVVRVKWEKVD-GRFQFKEIEGSNETIEADLVLLAMGFLGPEATIAEKLGLEKDNRSNFKAQFGNFATSVDGIFAAGDCRRGQSLVVWAITEGRQAAAAVDKYLSRNEQDAAEDITPSGAGFVQP 2164
BLAST of glutamate synthase vs. SwissProt
Match: gi|114152810|sp|Q12680.2|GLT1_YEAST (RecName: Full=Glutamate synthase [NADH]; AltName: Full=NADH-GOGAT; Flags: Precursor) HSP 1 Score: 1880.14 bits (4869), Expect = 0.000e+0 Identity = 1030/2150 (47.91%), Postives = 1385/2150 (64.42%), Query Frame = 0
Query: 34 PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVG-KLGHRLPPLGQYATGILFLGPGN------LQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVV----RKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVTLGDMRKANKHEISTERRPIIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVL--GWKTKVIDITYEVSNAL-NLEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFE-IAEMLRE---ENVISSDITNEVA--YANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDP--GFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSE----VGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALD-GKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGV-GDHGCEYMTGGRVIILG-CTGRNFAAGMSGGIAYVLDHE-GNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEI--NGRVTPE-------------------IRVTNDK--EESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIIT-KNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLV-GDTLLPGLGGVIH 2127
P+K GLYDP E DACGVGF+ + G QSH ++ DA L M HRGA S+D + GDGAG+L+ IP EF ++E L +P +G+YA G +F L + + FE LA + +L VL WR VP ++ LG +A EP + Q +V K+P+ + F T ++LRK AS ++ + W FYV SL++TT+VYKGQ P Q++ Y+ DL + FK+H+ +VH+RFSTNTFPSW+RA P R LAHNGEINTLRGN N M++REGVM + F +L +L+P++E SDS ALDNVLE L + G SLPEAVM +VPEA+ D MD K +Y +A+C EPWDGPALLTF+DGRY GAILDRNGLRP RYY+T+D+ ++ ASEVGV + ++QKG+L PG + LVDT +++ + +K +I+ + ++W ++ + L D+ + + E ++ S+ ++ Q D R+ GYT E + L+ PM T KEALGSMGND PLACL++ L+Y+YF+QLFAQVTNPPIDP RE VMSLEC +GP+GNLLE + C RL LK+PIL + + LK W IDIT++ S L D +DKI K A +A + + +I++DR +G R+ IS+ +A +HHHLI + RS+ A+ILETGE RE+H CV++G+G D + PY+ E + M RE NV + + T E NY A+D GI KVM+KMGIST+ SYK AQIFEA+GL N +VD CF GT+SRI GVTF LA+++ H+ + + S+ + P G H+R GG KH+N+PT++A LQ+ + +N S+Q YV +E+IRDCT+RG LE++F + S +I +++VEP +EI +RFA+GAMS+GSIS EAHSTLA+AMN+LG KSN GEGGE+ ER Q+ MRSAIKQVAS RFGVT+ YL++ADE+QIK+AQGAKPGEGGELP KV+ DIAKTRHS P VGLISPPPHHDIYSIEDL QLIYDLKC NP A ISVKLVSEVGVG+VASGVAK K++HIL+SGHDGGTGA+ WT +K AGLPWELGL+ETHQ L +N+LR + Q L+ T ++V L +E V ++A G + H L S G + K G P + + + + + +++ GF + DE + L ++ ++ ++ ILT A ++RPG + +QD +L RLD ++I + LD G P + I+ I N RA G+TLS+ ++K++ + GLP+ + + ++GSAGQSF FL GI L GDANDYVGKGLSGG ++I PPK+S FKS+EN+I GN C YGATSG AFI G A ERF VRNSGA +VE + G++ EYMTGGR I+L N +G +GGIAY L + +F K NK+ V+L + D +I F+K +QE + T S +A IL N++ + FVKV P +Y++ LL +KA + + + + E K+ N V E I ++N +E V D+EDAV D + K E I +KTRGF+ +KR + +RD R DW+E + +K K Q ARCMDCG PFC S + GCPL NIIPKFN+L+F + WK ALD+LL+TNNFPEFTGRVCPAPCEGAC LGI E V IK++E IIDNAF+EGWIKP PP R+G TV ++GSGPAGLA AD LN+AGH VTVYER++R GGLL YGIP MKL K +VQRR+DL++ EGI F N ++GK I++D++KN ++AV+ +G+T PRDLPI RE + I FAM+ L++ + +++ + K VIV+GGGDTG DC+GTS+R GA S+ +FE+LP PP R+ DNPWPQ+PR+ +VDYGH EV +G+ DPR Y S EF+ ++ ++T IRTV+VEW K +G W + SE+I+E + LL+MGF+GPE +++ R I T + Y+ FA GDCRRGQSL+VWAI EGR+ A VD+FL+ G T LP GG++
Sbjct: 39 PDKRGLYDPDYEHDACGVGFVANKHGEQSHKIVTDARYLLVNMTHRGAVSSDGN-GDGAGILLGIPHEFMKREFKLDLDLDIPEMGKYAVGNVFFKKNEKNNKKNLIKCQKIFEDLAASFNLSVLGWRNVPVDSTILGDVALSREPTILQPLLVPLYDEKQPEF--NETKFRTQLYLLRKEASLQIGLEN--W-------FYVCSLNNTTIVYKGQLTPAQVYNYYPDLTNAHFKSHMALVHSRFSTNTFPSWDRAQPLRWLAHNGEINTLRGNKNWMRSREGVMNSATFKDELDKLYPIIEEGGSDSAALDNVLELLTINGTLSLPEAVMMMVPEAYHKD--MDSDLKAWYDWAACLMEPWDGPALLTFTDGRYCGAILDRNGLRPCRYYITSDDRVICASEVGVIPIENSLVVQKGKLKPGDLFLVDTQLGEMVDTKKLKSQISKRQDFKSWLSKVIKLDDLLSKTANLVPKE---------FISQDSLSLKV-QSDPRLLANGYTFEQVTFLLTPMALTGKEALGSMGNDAPLACLNENPVLLYDYFRQLFAQVTNPPIDPIREANVMSLECYVGPQGNLLEMHSSQCDRLLLKSPILHWNEFQALKNIEAAYPSWSVAEIDITFDKSEGLLGYTDTIDKITKLASEAIDDGKKILIITDRKMGANRVSISSLIAISCIHHHLIRNKQRSQVALILETGEAREIHHFCVLLGYGCDGVYPYLAMETLVRMNREGLLRNVNNDNDTLEEGQILENYKHAIDAGILKVMSKMGISTLASYKGAQIFEALGLDNSIVDLCFTGTSSRIRGVTFEYLAQDAFSLHERGYPSRQTISKSVNLPESGEYHFRDGGYKHVNEPTAIASLQDTVRNKNDVSWQLYVKKEMEAIRDCTLRGLLELDF--ENSVSIPLEQVEPWTEIARRFASGAMSYGSISMEAHSTLAIAMNRLGAKSNCGEGGEDAERSAVQENGDTMRSAIKQVASARFGVTSYYLSDADEIQIKIAQGAKPGEGGELPAHKVSKDIAKTRHSTPNVGLISPPPHHDIYSIEDLKQLIYDLKCANPRAGISVKLVSEVGVGIVASGVAKAKADHILVSGHDGGTGAARWTSVKYAGLPWELGLAETHQTLVLNDLRRNVVVQTDGQLR-TGFDIAVAVLLGAESFTLATVPLIAMGCVMLRRCH-LNSCAVGIATQDPYLRSKFKGQPEHVINFFYYLIQDLRQIMAKL----------GFRTI-------DEMVGHSEKLKKRDDVNAKAIN-IDLSPILTPAHVIRPGVPT-KFTKKQDHKLHTRLDNKLIDEAEVTLDRGLP--VNIDASIINTDRALGSTLSYRVSKKFGEDGLPKDTVV-VNIEGSAGQSFGAFLASGITFILNGDANDYVGKGLSGGIIVIKPPKDSK--FKSDENVIVGNTCFYGATSGTAFISGSAGERFGVRNSGATIVVERIKGNNAFEYMTGGRAIVLSQMESLNAFSGATGGIAYCLTSDYDDFVGKINKDTVELESLCDPVEIAFVKNLIQEHWNYTQSDLAARILGNFNHYLKDFVKVIPTDYKKVLLKEKAEAAKAKAKATSEYLKKFRSNQEVDDEVNTLLIANQKAKEQEKKKSITISNKATLKEPKVVDLEDAVPDSKQLEKNSERI-EKTRGFMIHKRRHETHRDPRTRVNDWKEFTN-PITKKDAKYQTARCMDCGTPFCLSDT-GCPLSNIIPKFNELLFKNQWKLALDKLLETNNFPEFTGRVCPAPCEGACTLGIIEDPVGIKSVERIIIDNAFKEGWIKPCPPSTRTGFTVGVIGSGPAGLACADMLNRAGHTVTVYERSDRCGGLLMYGIPNMKLDKAIVQRRIDLLSAEGIDFVTNTEIGKTISMDELKNKHNAVVYAIGSTIPRDLPIKGRELKNIDFAMQLLESNTKALLNKDLEIIREKIQGKKVIVVGGGDTGNDCLGTSVRHGAASVLNFELLPEPPVERAKDNPWPQWPRVMRVDYGHAEVKEHYGR-DPREYCILSKEFIGNDEGEVTAIRTVRVEWKKSQSGVWQMVEIPNSEEIFEADIILLSMGFVGPELINGNDNEVKKTRRGTIATLDDSSYSIDGGKTFACGDCRRGQSLIVWAIQEGRKCAASVDKFLMDGTTYLPSNGGIVQ 2132
BLAST of glutamate synthase vs. SwissProt
Match: gi|1707984|sp|P55037.1|GLTB_SYNY3 (RecName: Full=Ferredoxin-dependent glutamate synthase 1; AltName: Full=Fd-GOGAT) HSP 1 Score: 1279.23 bits (3309), Expect = 0.000e+0 Identity = 714/1584 (45.08%), Postives = 979/1584 (61.81%), Query Frame = 0
Query: 18 LLPNPVMPFDPDRPRY--PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLGPGNLQ--ESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVTLGDMRKANKHEISTERRPIIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNA-LNLEDALDKICKEAEKA-AREHQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAAL---ESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYL-NQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVG---VVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLG--ADEFGFSTAPLIAMGSTD--SPKVDLLNFEK--ILTNASLLRPGASILGG---SVEQDFQLEARLD-AEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASE 1578
L+PN P + P+ GLYDP E DACGVGFIV + G SHD++ Q+ +EHRGAC + ++GDGAG+L+ +P +F +K G G +P GQYA G ++ P L E++ +F + LKVL WR +P +N+ LG A SEP M+QV++ R P+ L +D F +V+RK+ H + + D +YV+SLS+ TLVYKG Q+ QY+ +L DPD ++ + +VH+RFSTNTFPSWER+HP R +AHNGEINT+RGN+N M+AR+ + + FG ++++ PV+ D SDS DN LE L +AG RSLP AVM ++PE W M + KK FY++ SC EPWDGPA + F++G+ +GA+LDRNGLRPSRYYVT D++++MASE GV +P ++ +KGRL PGRM LVD + +II DE +K+EI P+ W ++ S E+ P ++ + S+ +R FGYT E +++L+ PM EA+GSMG DTPLA LS L+Y YF+QLFAQVTNPPID RE I+ S E IG EGNLL+P +C+ + LK PIL+ DL LK +K+ +DI ++ + L+ ALD + EA++A ++ IILSDR + + I A LA +HHHLI R+K ++LE+GE REVH V++G+G AI PY+ FE + + E ++ ++ ++ A NY A +G+ KV +K+GIST+QSY+ AQIFEAVGL+ V+D F T+SRI G ++A+E+++RH+ AF D L G +R GE+H+ P ++ LLQ A + N Y++Y AAL ++ + T+RG L+ +D+ ++I ++EVEP I+KRF TGAMS+GSIS EAH +LA+AMN++GGKSNTGEGGE+PER+ D + SAIKQVASGRFGVT+ YL+ A E+QIKMAQGAKPGEGG+LPG KV IAK RHS PGVGLISPPPHHDIYSIEDLA+LI+DLK N ARI+VKLVSEVGVG +A+GVAK ++ +L+SG+DGGTGAS T IK AGLPWELGL+ETHQ L +NNLRSR + + +K A ++ E G +V+ G ++ H L+ + N R D F +A L + GF T + +G TD PK + +++ I + L +P G + QD L+ LD +++ +CQ A+ K + I N R G + +EI KR+ + GLPE + + +GSAGQSF F+ KG+ + LEGDANDY+GKGLSGG++I++PPK S +F + ENIIAGNVCLYGAT+G+ +I G+ ERFCVRNSG +VE VGDHGCEYMTGG+V++LG TGRNFAAGMSGG+AY+ D G+F +CN MV L + D E+I LKE +Q V+ T S +L +W ++ FVKV PR+Y+R L K A E
Sbjct: 10 LVPNFCTVTSPMNSSHLAPQVQGLYDPQNEHDACGVGFIVQMKGKVSHDIVEQGLQMLVNLEHRGACGCEPNTGDGAGILIQVPHKFIQKIAGAEGITIPAPGQYAVGNIYGSPDPLARAEARQKFNDIVAQEGLKVLGWRDIPTQNEPLGETAIASEPFMQQVYIAR--PEGLTDDLDFERKLYVIRKLT------HGAIRSPKIDTYWYVASLSARTLVYKGMLTTAQVGQYYPELHDPDMESALALVHSRFSTNTFPSWERSHPYRYIAHNGEINTMRGNVNWMQARQALFESSLFGEDMAKVQPVINIDGSDSTIFDNALELLYLAG-RSLPHAVMMMIPEPWSAHESMSQEKKAFYKYHSCLMEPWDGPASIAFTNGKMMGAVLDRNGLRPSRYYVTKDDLVIMASEAGVLPIEPERVAKKGRLQPGRMFLVDMEQGRIIADEEIKQEIVSQHPYGEWLAANLK------------SLEQLPSPGNVPGTDAESL-------RQRQMAFGYTFEELRILLAPMGRDGVEAIGSMGADTPLAVLSDKPKLLYNYFQQLFAQVTNPPIDSIREEIITSAETTIGGEGNLLDPRPESCRLIELKTPILTNEDLAKLKALDDDEFKSVTLDILFDPNQGEAGLKTALDNLFTEADQAISQGANLIILSDRQVSAEKAAIPALLAVSGLHHHLIRNGSRTKVGLVLESGEPREVHHFAVLLGYGCGAINPYLAFETLDGMIAEGLLV-NVDHKTACKNYIKAATKGVIKVASKIGISTIQSYRGAQIFEAVGLNQSVIDEYFCRTSSRIQGSDLGVIAQEAILRHQHAFAPRPGDLHTLDVGGEYQWRKDGEEHLFSPQTIHLLQRAVREGNYELYKQY--AALVNEQNQKFFTLRGLLDF---QDR-ESIPLEEVEPIEAIMKRFKTGAMSYGSISKEAHESLAIAMNRIGGKSNTGEGGEDPERFTWTNDQGDSKNSAIKQVASGRFGVTSLYLSQAKEIQIKMAQGAKPGEGGQLPGKKVYPWIAKVRHSTPGVGLISPPPHHDIYSIEDLAELIHDLKNANREARINVKLVSEVGVGTIAAGVAKAHADVVLVSGYDGGTGASPQTSIKHAGLPWELGLAETHQTLVLNNLRSRIVVETDGQMKTGRDVAIAALLGAEEFGFSTAPLVSLGCIMMRACH----------------------------LNTCPVGIATQNPELRAKFTGDPAHAVNFMTFIATELREVMAQLGFRTINEM-VGRTDILEPKKAVAHWKAKGIDLSTILHQPEVGDDVGRYCQIPQDHGLQHSLDITQLLDLCQPAI-AKGEKVTATLPITNINRVVGTIVGNEITKRHWE-GLPEDT-VHLHFQGSAGQSFGAFIPKGMTLELEGDANDYLGKGLSGGKIIVYPPKGS--SFIASENIIAGNVCLYGATAGEVYISGMVGERFCVRNSGVNTVVEAVGDHGCEYMTGGKVVVLGQTGRNFAAGMSGGVAYIFDETGDFATRCNSAMVGLEKLEDPEEIKDLKELIQNHVNYTDSAKGKAVLADWEASIPKFVKVMPRDYKRVLQAIKKALE 1524
BLAST of glutamate synthase vs. SwissProt
Match: gi|46395959|sp|Q9C102.1|GLT1_SCHPO (RecName: Full=Putative glutamate synthase [NADPH]; AltName: Full=NADPH-GOGAT) HSP 1 Score: 1187.56 bits (3071), Expect = 0.000e+0 Identity = 592/1127 (52.53%), Postives = 774/1127 (68.68%), Query Frame = 0
Query: 34 PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLGPGN--LQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRK---EPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQH-VTLGDMRKANKHEISTERRPIIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVL--GWKTKVIDITYEVSNAL-NLEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENEC-DSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQ 1149
P+ LYDP+ EKD+CGVGF HI G SH ++ DA L M HRGA AD GDGAGV+ +P F +KE G++G LP G+YA G +F P +E+ F +A L L +L WR VP +N LG A EP + Q VV K + + + +F +VLRK +SH + +K FY+ SL T+VYKGQ P Q++ YFLDL + ++ +H +VH+RFSTNTFPSW+RA P R+ AHNGEINTLRGN N M AREG+M + +FG + + L P++E SDS A DNV+E L +G SLPEAVM L+PEAWQND + + K FY +A+C EPWDGPAL TF+DGRY GA LDRNGLRP R+Y+T+D++M+ ASEVG +P +I+QKGRL PGRMLLVDT E +I+ D+ +K IA +W +Q + + + + S + PI+ + L +D +++A FGYTLE I M+M PM N KE LGSMGND +ACLS L+Y+YF+QLFAQVTNPPIDP RE IVMSL+C IGP GNLLE N+ C+RL + PIL++ + LK + WK IDIT+ S + A+++IC EA+ A E ++ I+LSDRN+ R+P+++ A GAVHH+L+ +LRS+ A++ E+G+ REVH +C ++G+G DA+CPY+ E L +N + IT E A N+ A++ GI KVM+KMGIST+QSYK AQIFEA+G+ NEV+++CF GTASRI GVTF +A ++ H+ + ++ S + D G +YR GGE+H+N P ++A LQ+A + +N+A+Y ++ E R CT+RG L+ +F D S+AI I++VEP +EIV+RF TGAMS+GSIS E+HS+LA+AMN+LGGKSNTGEGGE+P R MRSAIKQ+ASGRFGVT+ YL++ADELQIKMAQGAKPGEGGELPG KV+ IAKTRHS GVGLISPPPHHDIYSIEDL QLIYD+K NP AR+SVKLVSEVGVG+VASGVAK K++HIL+SGHDGGTGAS WTGIK AGLPWELG++ETHQ L +N+LR R + Q
Sbjct: 54 PKAQALYDPAYEKDSCGVGFTCHIKGQVSHKIVTDARLLLCNMTHRGATGADTRDGDGAGVMTGMPYTFMQKEFGQIGCTLPKSGEYAIGNVFFSPEADVCREAMTAFTQVAEKLGLAILAWRSVPCDNSILGPAALSREPTILQPCVVLKAAYDGEAEFDTDLFERQLYVLRKQSSHLIG---------KEKWFYICSLHRETIVYKGQLAPVQVYNYFLDLNNAEYVSHFALVHSRFSTNTFPSWDRAQPMRLAAHNGEINTLRGNKNWMHAREGLMKSSRFGEEFASLLPIIERGGSDSAAFDNVIELLCASGVVSLPEAVMLLIPEAWQNDKNISDEKAAFYEWAACQMEPWDGPALFTFADGRYCGANLDRNGLRPCRFYLTSDDMMICASEVGTVGIEPDRIVQKGRLYPGRMLLVDTKEGRIVDDKELKHNIASRYDFRSWLDQELIDMNSIVDSLIESTSVDLTPIVDDVPLADDKTMLA-----------FGYTLEQINMIMAPMANGGKETLGSMGNDAAIACLSDQPRLLYDYFRQLFAQVTNPPIDPIREAIVMSLQCYIGPSGNLLEINQSQCRRLRMPTPILTVEEFNALKNVDRIYPDWKVASIDITFFKSEGVAGYAAAIERICSEADTAVNEGYKAIVLSDRNVNSERVPLASIAACGAVHHYLVQNKLRSRVALVCESGDAREVHHMCTLLGYGADAVCPYLAMEALTKLVRQNAMKPGITEETAIKNFKHAINGGILKVMSKMGISTLQSYKGAQIFEALGIDNEVINKCFLGTASRIRGVTFEHIALDAFALHERGYPTDQSIRSLQIPDMGDFYYRDGGEQHVNHPKAIASLQDAVRNKNEAAYAEFSRTHYEQTRRCTLRGMLDFDF--DSSQAIPIEQVEPWTEIVRRFCTGAMSYGSISMESHSSLAIAMNRLGGKSNTGEGGEDPARSQRLANGDTMRSAIKQIASGRFGVTSWYLSDADELQIKMAQGAKPGEGGELPGNKVSESIAKTRHSTAGVGLISPPPHHDIYSIEDLKQLIYDMKSANPRARVSVKLVSEVGVGIVASGVAKAKADHILVSGHDGGTGASRWTGIKYAGLPWELGVAETHQTLVLNDLRGRVVIQ 1158 HSP 2 Score: 883.248 bits (2281), Expect = 0.000e+0 Identity = 459/844 (54.38%), Postives = 582/844 (68.96%), Query Frame = 0
Query: 1287 ASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALD-GKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGK--DITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKM-DLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGDTLLPGLGGVI 2126
A LRPGA+ +QD +L RLD ++I + L+ G P V+E EI N R GATLS++I+KRY + GLP I + + GSAGQSF FL G+ + LEGD NDYVGKGLSGG +II+PP+ S FK EEN+I GNVCLYGATSG AFI GVAAERF VRNSGA+A+VEGVGDHGCEYMTGGRV+ILG TGRNFAAGMSGGIAYV D + +F K N EMVD+ + D +I FL+ +Q+ TGS VAD IL ++ FVKV PREY +A+L+++AA ++ + ++ P + G IR ++ + +AD+ED + K+ LDK RGF+KY+R + YR+ R DW+E+ + L+ Q ARCMDCG PFC S +GCP+ N I +NDLVF WKEAL QLL TNNFPEFTGRVCPAPCEGAC LGI E V IK++E AIID A+EEGWI P PP +R+G+ VAI+GSGPAGLAAADQLN+AGH V +YER +R GGLLQYGIP MKL K VV+RR+ LM EGI NV+VGK D+++D++ DAV+L G+T PRDLPIPNR+++GIHFAMEFL ++ ++ D + I+A KDVIVIGGGDTG DC+GTS+R GAKS+ + E+LP PP R+ DNPWPQ+PR+F+VDYGH EV +G+ D R Y+ + F KDE + GI TV++EW K+ GRW+ + + SE+ + L +LA+GFLGPE +++D RSNI T Y T G++AAGDCRRGQSLVVW I EGRQ ARE+D G T LPG GG++
Sbjct: 1280 AFTLRPGAATYNVR-KQDHRLYTRLDNKLIDEAEVTLEEGIPS--VVECEIINTDRTLGATLSNKISKRYGEEGLPTD-SIRVNVFGSAGQSFGAFLAPGVTLQLEGDCNDYVGKGLSGGRLIIYPPRVS--PFKPEENMIIGNVCLYGATSGHAFISGVAAERFAVRNSGAIAVVEGVGDHGCEYMTGGRVVILGSTGRNFAAGMSGGIAYVYDMQMDFAGKINTEMVDISSVTDAAEIAFLRGLIQDHRHYTGSQVADRILSDFPRHLSRFVKVLPREY-KAVLEREAAKKE-EAKRLQYPKAFMPGN---PIRQQIEETNAQIADVEDTLG----ATVKKSAPLDKLRGFMKYQRRSEHYRNPLKRTNDWKEL-SVRLREDELRVQTARCMDCGTPFCQS-DYGCPISNKIFTWNDLVFKQQWKEALTQLLLTNNFPEFTGRVCPAPCEGACTLGIIESPVGIKSVERAIIDKAWEEGWIVPRPPAERTGRRVAIIGSGPAGLAAADQLNRAGHHVVIYERADRPGGLLQYGIPNMKLDKKVVERRIQLMIDEGIEVLTNVEVGKNGDVSLDELHKVYDAVVLASGSTVPRDLPIPNRDSKGIHFAMEFLHKNTKSLLDSELKDGNYISAKGKDVIVIGGGDTGNDCLGTSVRHGAKSVRNLELLPIPPRERAFDNPWPQYPRVFRVDYGHAEVQAHYGQ-DFREYSILTKSFEKDEDGNVKGINTVRIEWTKNSKGRWIMKEIRNSEEFFPADLVILALGFLGPEEQATAGMNVDRDARSNISTPTKSYETSVPGIYAAGDCRRGQSLVVWGIQEGRQCAREIDLKFQGKTFLPGDGGLV 2105 HSP 3 Score: 196.438 bits (498), Expect = 9.447e-49 Identity = 91/123 (73.98%), Postives = 108/123 (87.80%), Query Frame = 0
Query: 1146 DLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMG 1268
DL QLIYD+K NP AR+SVKLVSEVGVG+VASGVAK K++HIL+SGHDGGTGAS WTGIK AGLPWELG++ETHQ L +N+LR RVV+Q DGQ+RTG DV +A LLGA+E+GF+T PLIA+G
Sbjct: 1069 DLKQLIYDMKSANPRARVSVKLVSEVGVGIVASGVAKAKADHILVSGHDGGTGASRWTGIKYAGLPWELGVAETHQTLVLNDLRGRVVIQTDGQIRTGRDVAIACLLGAEEWGFATTPLIALG 1191
BLAST of glutamate synthase vs. SwissProt
Match: gi|300680981|sp|Q9LV03.2|GLUT1_ARATH (RecName: Full=Glutamate synthase 1 [NADH], chloroplastic; AltName: Full=NADH-dependent glutamate synthase 1; Short=NADH-GOGAT 1; Flags: Precursor) HSP 1 Score: 1129.39 bits (2920), Expect = 0.000e+0 Identity = 599/1169 (51.24%), Postives = 777/1169 (66.47%), Query Frame = 0
Query: 34 PEKA-GLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLGP--GNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRK--MASHR--LDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFL-DLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLF---PVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWN-QHVTLGDMRKANKHEISTER-RPIIKSL--ALLNDHSIVARIWQGDRR-MPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNALN-LEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEV-----AYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAF-----GENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERY--LNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVG 1172
PEK GLYDPS +KD+CGVGF+ + G + + D+ ++ RM HRGAC ++++GDGAG+LV +P +F+ + +LG LP G YA G+ FL +ESK+ F +A +L VL WR VP +N LG A ++EPI+ QVF+ K F ++LR+ M + R L+ HG K FY+ SLSS T+VYKGQ P QL Y+ DL F +++ +VH+RFSTNTFPSW+RA P RVL HNGEINTLRGN+N M+AREG++ + G +L P+V+ +SDSGA D VLE LV AG RSLPEAVM ++PEAWQND +D ++K FY + S EPWDGPAL++F+DGRY+GA LDRNGLRP R+Y+T ++MASEVGV D P +++KGRL PG MLLVD + ++ D+ +K++ +L+RP+ W Q + L D+ ++ ER P I + A +D S+ + G + FGYT+E ++ML++PM EALGSMGNDTPLA +S + L +EYFKQ+FAQVTNPPIDP RE+IV S+EC IGPEG+L E E C RL LK P+L + ++E +KK GW+TKV+DITY LE+ LD+IC EA +A +E + ++LSDR TR+ +S+ +A GAVHHHL+ T R++ +++E+ E REVH C +VGFG DAICPY+ E L+ + I E Y A + G+ KV+AKMGIST+ SYK AQIFEA+GLS+EV+ +CF GT SR+ G TF +LA + L H+LAF ++ L +PG H+R GE H+NDP ++A LQEA + + A+Y++Y E + +RG ++ +D I +DEVEPASEIVKRF TGAMS+GSIS EAH+TLA+AMNKLGGKSNTGEGGE P R L RS+IKQ+ASGRFGV++ YL NADELQIKMAQGAKPGEGGELPG KV DIA TR+S GVGLISPPPHHDIYSIEDLAQLI+DLK NP ARISVKLVSE GVGV+ASGV KG ++H+LI+GHDGGTGAS WTGIK AGLPWELGL+ETHQ L N+LR R + Q LK A ++ E G
Sbjct: 101 PEKPLGLYDPSYDKDSCGVGFVAELSGETTRKTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEAATELGFVLPSAGNYAVGMFFLPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLT----PTTKSKADFEQQMYILRRVSMVAIRAALNLQHGAMKD-----FYICSLSSRTIVYKGQLKPDQLKDYYYADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAG-RSLPEAVMMMIPEAWQNDKNIDPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKDIIESVPE---AERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVYRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHELAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGLMKF---KDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLADGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFG 1253 HSP 2 Score: 868.996 bits (2244), Expect = 0.000e+0 Identity = 450/841 (53.51%), Postives = 582/841 (69.20%), Query Frame = 0
Query: 1289 LLRPGASILGGSVE-----QDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTN---DKEESPVADIEDAVKDKDMVAKKQEVILD---KTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSK-DVVQRRVDLMAQEGIIFKANVDVGKD--ITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQN-QHGDKMDLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLV 2114
LLRP A I G+ + QD L+ LD E+I + + AL+ K + IE I N RA G LSHE+ KRY +GLP+ I I+ GSAGQS FL GI + LEGD+NDYVGKGLSGG+V+++PPK S +F +ENI+ GNV LYGATSG+A+ G+AAERF VRNSGA A+VEG+GDHGCEYMTGG V++LG TGRNFAAGMSGGIAYVLD +G F +CN E+VDL + D+ED + LK +Q+ T S +A +L ++ + F+KVFPR+Y+R L K E+ S +IE +E + E+ + + + A ++ + + A+ + +D K GF+ Y+RE YRD R DW E+ + L Q+ARCMDCG PFCH + GCPLGN IP+FN+LV+ + W+EAL++LL+TNNFPEFTGRVCPAPCEG+CVLGI E V+IK+IECAIID AFEEGW+ P PP KR+GK VAI+GSGPAGLAAADQLNK GHLVTVYER++RIGGL+ YG+P MK K DVVQRRVDLM +EGI F N ++GKD ++D +K NDA++L VG+T PRDLP+P R+ G+HFAMEFL ++ + D + I+A K V+VIGGGDTG DCIGTS+R G +I + E+LP PP TR+ NPWPQ+PR+F++DYGH+E + K+GK DPR Y + F+ D+ + G+ V+V W KD+ GR+ + +EGSE+I E L LAMGFLGPE + + GLE D RSN + G ++T GVFAAGDCRRGQSLVVWAI+EGRQAA +VD+FL
Sbjct: 1351 LLRPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALE-KSLPVYIETPICNVNRAVGTMLSHEVTKRYHLTGLPKDT-IHIKFTGSAGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKVVVYPPKGS--SFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGLGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFNTRCNLELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLADFENLLPKFIKVFPRDYKRVLSAMK--HEEVSKQAIERASEEADETEEKELEEKDAFAELKNMAAASSKEEMSGNGVAAEARPSKVDNAVKNGGFIAYEREGVKYRDPNVRLNDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMVPRPPLKRTGKKVAIIGSGPAGLAAADQLNKMGHLVTVYERSDRIGGLMMYGVPNMKTDKIDVVQRRVDLMTKEGINFVVNANIGKDPSYSLDGLKEENDAIVLAVGSTKPRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEATTKFGK-DPRTYEVLTKRFIGDDNGNVKGLELVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAADQVDKFLT 2184 HSP 3 Score: 197.593 bits (501), Expect = 4.724e-49 Identity = 94/123 (76.42%), Postives = 106/123 (86.18%), Query Frame = 0
Query: 1146 DLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMG 1268
DLAQLI+DLK NP ARISVKLVSE GVGV+ASGV KG ++H+LI+GHDGGTGAS WTGIK AGLPWELGL+ETHQ L N+LR R VLQ DGQ++TG DV VAALLGA+EFGFSTAPLI +G
Sbjct: 1141 DLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLG 1263
BLAST of glutamate synthase vs. SwissProt
Match: gi|12644435|sp|Q43155.3|GLTB_SPIOL (RecName: Full=Ferredoxin-dependent glutamate synthase, chloroplastic; AltName: Full=Fd-GOGAT; Short=SoFdGOGAT) HSP 1 Score: 1015.76 bits (2625), Expect = 0.000e+0 Identity = 631/1562 (40.40%), Postives = 890/1562 (56.98%), Query Frame = 0
Query: 49 CGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLGPGNLQESKDEF---EALALTLD------LKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGM--FVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDP-LMDETKK--NFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVTLGDMRKANKHEISTERRPIIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNAL--NLEDALDKICKEAEKAARE-HQFIILSDRN--IGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISS--------DITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPER----------YLNQDPDY-------NMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQM-RTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEII--PICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFP 1563
CGVGFI ++D S +++DA MEHRG C +DNDSGDG+GV+ AIP + F + GK + P + TG+ G + KD+ EA + LD ++V+ WR VP +G AK++ P ++QVFV ++KED ++ RK+ H W + Y SLS+ T++YKG L ++ DL + + + I H R+STNT P W A P R L HNGEINT++GN+N M++RE + + + + +++ P SDS LD+ E L+ +G R+ EA+M LVPEA++N P LM + + +FY + E WDGPALL FSDG+ VGA LDRNGL P+RY+ T DN++ +ASEVGV D K+ KGRL PG M+ VD ++ ++ +KK +A S P+ W ++ L ++ N S ALL + +I+ R FGY+ E +QM++ M + KE MG+D PLA +S+ ++Y+YFKQ FAQVTNP IDP RE +VMSLE IG GN+LE N ++ L +P+L+ +LE L +L K +++ I +++ + LE L+++C+ A++A R Q ++LSDR+ + TR I LA GAVH HLI LR +I+++T + HQ ++G+G AICPY+ E R N + +T E A N+ AV G+ K+++KMGIS + SY AQIFE GL +VVD F G+ S++GG+T LA E+L AF E+ ++ L + GF+ +R GGE H+N+P LL +A + +++++Y Y L + +R LE F D++ I + +VEPA+ IV+RF TG MS G+IS E H +A+AMN+LGGKSN+GEGGE+P R Y + P SAIKQVASGRFGVT ++L NAD+++IK+AQGAKPGEGG+LPG KV+ IA+ R+S PGV LISPPPHHDIYSIEDLAQLIYDL NP A++SVKLV+E G+G VASGVAKG ++ I +SGHDGGTGAS + IK AG PWELGLSETHQ L N LR R + ++ LKC + E G G +A +A G + ++ G +S A P G + N V + G + + GF+ L + D L+ K L+ IL +A L P S ++ LD +I+ P DA++ + + +I N RA ++ IAK+Y D+G Q+++ +GSAGQSF VFL G+ + L G++NDYVGKG++GGE+I+ P +N F+ E+ I GN CLYGAT G+ F+RG A ERF VRNS A A+VEG GDH CEYMTGG V+ILG GRN AAGM+GG+AY+LD + K NKE+V + + + LK ++ V+KTGS +ILK+W +F ++ P
Sbjct: 1 CGVGFIANLDNKGSFQIVKDALTALGCMEHRGGCGSDNDSGDGSGVMTAIPWDLF-NDWGK-DQGIGPFDRSHTGV-----GMVFLPKDDLLAEEAKKVVLDTFAQEGIEVIGWRSVPTNVSVVGRNAKETMPNIQQVFV-----RIIKEDSTDDIERELYICRKLIERAASSH--TWASE----LYFCSLSNQTIIYKGMLRSEVLGMFYYDLQNERYTSPFAIYHRRYSTNTSPRWPLAQPMRFLGHNGEINTIQGNLNWMRSREPSIQSPVWRGRENEIRPYGNPKASDSANLDSAAELLIRSG-RTPEEALMILVPEAYKNHPTLMIKYPEAVDFYDYYKGQMETWDGPALLLFSDGKTVGACLDRNGLAPARYWRTVDNVVYVASEVGVLPMDESKVTMKGRLGPGMMISVDLSSGQVYENTEVKKRVASSNPYGKWVKEN--LRSLKAVN-----------FLSRALLENDTIL-------RNQQAFGYSSEDVQMVIESMASQGKEPTFCMGDDIPLAVMSQKPHMLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILEVGPENASQVILPSPVLNEGELEALVNDPLL--KAQMLPIFFDIRKGVEGTLEKRLNRLCEAADEAVRNGSQMLVLSDRSEELEPTRPAIPILLAVGAVHQHLIQNGLRMYTSIVVDTAQCFSTHQFACLIGYGASAICPYLALETCRQWRLSNKTVNLMRTGKIPTVTIEQAQNNFCKAVKSGLLKILSKMGISLLSSYCGAQIFEIYGLGKDVVDIAFQGSVSKMGGLTLDELARETLSFWVKAFSEDT--AKRLENFGFIQFRPGGEYHVNNPEMSKLLHKAVRNKSESAYAVY-QQHLANRPVSVLRDLLE--FKSDRAP-ISVGKVEPATSIVERFCTGGMSLGAISRETHEAIAIAMNRLGGKSNSGEGGEDPIRWRPLTDVVDGYSSTLPHLKGLQNGDTATSAIKQVASGRFGVTPTFLVNADQIEIKIAQGAKPGEGGQLPGKKVSAYIARLRNSKPGVPLISPPPHHDIYSIEDLAQLIYDLHQINPKAKVSVKLVAEAGIGTVASGVAKGNADIIQVSGHDGGTGASPISSIKHAGGPWELGLSETHQTLISNGLRERVILRVDGGLKCGVDVMMAAAMGADEYGFGSLAM-IATGCVMARICHTNNCPVGVASQREELRARFPGVPG--------DLVNFFLYVAEEVRGILAQLGFEKLDDIIGRTDILKPRDISLM--------KTQHLDLSYILASAGL--PTMSSTAIRKQEVHTNGPVLDDQILSDPEIIDAIENEK-IVNKTVKIFNVDRAVCGRIAGVIAKKYGDTGF--AGQLNLTFEGSAGQSFAVFLTPGMNIRLVGESNDYVGKGMAGGELIVTPAENP--GFRPEDATIVGNTCLYGATGGQIFVRGKAGERFAVRNSLAEAVVEGTGDHCCEYMTGGCVVILGKVGRNVAAGMTGGLAYILDEDDTLIPKVNKEIVKIQRVTAPVGQMQLKNLIEAHVEKTGSSKGASILKDWDKYLPLFWQLVP 1491
BLAST of glutamate synthase vs. SwissProt
Match: gi|313104232|sp|P09831.3|GLTB_ECOLI (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=Glutamate synthase subunit alpha; Short=GLTS alpha chain; AltName: Full=NADPH-GOGAT; Flags: Precursor) HSP 1 Score: 1011.13 bits (2613), Expect = 0.000e+0 Identity = 638/1567 (40.71%), Postives = 866/1567 (55.26%), Query Frame = 0
Query: 39 LYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLG--PGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVT-LGDMRKANKHEISTERRPIIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNALNLEDALDKICKEAEKAAREHQ-FIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEF-GFSTAPLIA----------MGSTD--------SPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPI----IDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQ-RALLDQKAAS 1577
LYD SLE+D CG G I HI+G SH ++R A RM+HRGA AD +GDG G+L+ P FF + G RL YA G+LFL P ++ E L ++ WR VP LG IA S P +EQ+FV P + M F+ R+ RL + DK FYV SLS+ +YKG P L +++LDL D ++ IC+ H RFSTNT P W A P R LAHNGEINT+ GN +AR L P V SDS ++DN+LE L++AG + A+ LVP AWQN+P MD + F+ F S EPWDGPA + SDGR+ LDRNGLRP+RY +T D ++ ASEVG++D P ++++KGR+ PG ++++DT +I+ ++ P++ W ++V L E+ + L+D ++ + Q F Y+ E + ++ + +EA+GSMG+DTP A LS +IY+YF+Q FAQVTNPPIDP RE VMSL IG E N+ E RL K+PIL +D + L ++ +DIT++V+ LE + ++C +AEK R ++LSDRNI + R+P+ A +A GA+ L++ LR II+ET R+ H V++GFG AI PY+ +E L + + I+ D NY +++G+ K+M+KMGIST+ SY+ +++FEAVGL ++VV CF G SRIGG +F ++ L K A+ + + G L Y GGE H +P V LQ+A + + YQ+Y E T+R L I E+ A++I +VEPASE+ KRF T AMS G++S EAH LA AMN +GG SN+GEGGE+P RY N S IKQVASGRFGVT +YL NAD +QIK+AQGAKPGEGG+LPG KVT IAK R+SVPGV LISPPPHHDIYSIEDLAQLI+DLK NP A ISVKLVSE GVG +A+GVAK ++ I I+G+DGGTGAS + +K AG PWELGL ET Q L N LR + R+ V + GV ++ + + +S GTG G K ++ +NN + V Q D + + L + EF T L+A +G TD + K L K+L A PG ++ F L+A+++ + +D + +I N R+ GA+LS IA+ + D GL I G+AGQSF V+ G+++ L GDANDYVGKG++GG + I PP S AF+S E I GN CLYGAT G+ + G A ERF VRNSGA+ +VEG+GD+GCEYMTGG V ILG TG NF AGM+GG AYVLD G+F+ + N E+V++L + I +E L+ + E V TGS + IL NWS+ F V P+ +ALL ++ S
Sbjct: 2 LYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGAILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAK--NYAVGMLFLNKDPELAAAARRIVEEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFV--NAPAGWRPRDM-ERRLFIARRRIEKRL---------EADKDFYVCSLSNLVNIYKGLCMPTDLPRFYLDLADLRLESAICLFHQRFSTNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLI-PDLHDAAPFVNETGSDSSSMDNMLE-LLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWDGPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVGPGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRE---------LDDDTLASYQKQ-------FNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQQFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLTTMKEEHYRADTLDITFDVTKT-TLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLPVPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETLGRLVDTHAIAKDY--RTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDVVGLCFQGAVSRIGGASFEDFQQDLLNLSKRAW----LARKPISQGGLLKYVHGGEYHAYNPDVVRTLQQAVQSGEYSDYQEYAKLVNER-PATTLRDLLAITPGEN---AVNIADVEPASELFKRFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARY-----GTNKVSRIKQVASGRFGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHHDIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGTGASPLSSVKYAGCPWELGLVETQQALVANGLRHK---------------IRLQVDGGLKTGVDIIKAAILGAESFGF-------GTGPMVALGCK------------YLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARETRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAE-PHPGKALYCTENNPPFD-NGLLNAQLLQQAKPFVDERQSK-TFWFDIRNTDRSVGASLSGYIAQTHGDQGLAAD-PIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGS--AFRSHEASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCILGKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDALAIHEE---HLRGLITEHVQHTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRS 1477
BLAST of glutamate synthase vs. SwissProt
Match: gi|121446|sp|P23225.1|GLTB_MAIZE (RecName: Full=Ferredoxin-dependent glutamate synthase, chloroplastic; AltName: Full=Fd-GOGAT; Flags: Precursor) HSP 1 Score: 1009.98 bits (2610), Expect = 0.000e+0 Identity = 634/1610 (39.38%), Postives = 894/1610 (55.53%), Query Frame = 0
Query: 28 PDRPRYP--EKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFL--GPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFV-VRKEP---DVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMD---ETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVTLGDMRKANKHEISTERRPIIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNALN--LEDALDKICKEAEKAARE-HQFIILSDRNIGE--TRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISS--------DITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDY------------------NMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEII---PICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFP----------REYQRALLDQKAASEQTSS 1582
P P+ P + A L E+ ACGVGF+ ++ S D++RDA MEHRG C AD+DSGDGAG++ A+P + F+ K G L G++FL +++E+K E + + L+VL WR VP +G AK++ P ++Q+FV V KE D+ +E ++++ + R S W + Y SLSS T+VYKG L Q++LDL + +K+ I H RFSTNT P W A P R+L HNGEINT++GN+N M++RE + + + + ++ P + SDS LD+ E L+ +G RS EA+M LVPEA++N P + +FY + E WDGPALL FSDGR VGA LDRNGLRP+RY+ T+D+ + +ASEVGV D K++ KGRL PG M+ VD +++++ +KK +A + P+ W + L +P+ + + D+ V R Q FGY+ E +QM++ M + KE MG+D PLA LS+ L+Y+YFKQ FAQVTNP IDP RE +VMSLE IG GN+LE N ++ L +P+L+ +LE L + L K KV+ +++ L+ L+ + +C+EA+ A R Q ++LSDR+ TR I LA GA+H HLI LR +I+ +T + H ++G+G A+CPY+ E R N + +T E A N+ AV G+ K+++KMGIS + SY AQIFE GL EVVD F G+ S+IGG+T L E+L AF E+ ++ L + GF+ R GGE H N+P LL +A + + +Y Y L S +R LE+ D++ I I +VE A+ IV+RF TG MS G+IS E H +A+AMN++GGKSN+GEGGE+P R+ N D SAIKQVASGRFGVT ++L NAD+++IK+AQGAKPGEGG+LPG KV+ IA+ R+S PGV LISPPPHHDIYSIEDLAQLIYDL NP A++SVKLVSE G+G VASGV+K ++ I ISGHDGGTGAS + IK AG PWELGL+ET+Q L N LR R + ++ + + E G G VA +A G + ++ G +S A P G + + +R+ + + G++ L + D L+ K ++ +L+NA L +S + QD + E I P DA++ + + +I N RA ++ IAK+Y D+G Q++I GSAGQSF FL G+ + L G+ANDYVGKG++GGE+++ P + F E+ I GN CLYGAT G+ F+RG A ERF VRNS A+VEG GDH CEYMTGG V++LG GRN AAGM+GG+AY+LD + K NKE+V + + + LK ++ +V+KTGS IL+ W + +F ++ P E++R L Q A+ Q S+
Sbjct: 75 PPAPQKPTQQAADLNHILSERGACGVGFVANLKNMSSFDIVRDALMALGCMEHRGGCGADSDSGDGAGLMSAVPWDLFDDWASKQGLALFDRRNTGVGMVFLPQDEKSMEEAKAATEKVFVDEGLEVLGWRPVPFNVSVVGRNAKETMPNIQQIFVKVAKEDNADDIERE--LYISRKLIERAAKSFS-------WADE----LYFCSLSSRTIVYKGMLRSEVLGQFYLDLQNELYKSPFAIYHRRFSTNTSPRWPLAQPMRLLGHNGEINTIQGNLNWMRSRETTLKSPVWRGREHEICPFGDPKASDSANLDSTAELLLRSG-RSPAEALMILVPEAYKNHPTLSIKYPEVTDFYDYYKGQMEAWDGPALLLFSDGRTVGATLDRNGLRPARYWRTSDDFVYVASEVGVIPMDESKVVMKGRLGPGMMITVDLQTGQVLENTEVKKTVASASPYGTWLQECTRL--------------IKPVNFLSSTIMDNETVLRHQQA------FGYSSEDVQMVIESMASQGKEPTFCMGDDIPLAVLSQRPHLLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILEVGPENADQVALSSPVLNEGELETLLNDSKL--KPKVLSTYFDIRKGLDGSLDKTIQALCEEADAAVRSGSQLLVLSDRSEAPEPTRPAIPILLAVGAIHQHLIQNGLRMSASIVADTAQCFSTHHFACLIGYGASAVCPYLALETCRQWRLSNKTLNLMRNGKMPTVTIEQAQRNFIKAVKSGLLKILSKMGISLLSSYCGAQIFEIYGLGQEVVDLAFCGSVSKIGGLTLDELGRETLSFWVKAFSEDT--AKRLENFGFIQSRPGGEYHANNPEMSKLLHKAIREKRDNAYTVY-QQHLASRPVNVLRDLLELK--SDRAP-IPIGKVESATSIVERFCTGGMSLGAISRETHEAIAIAMNRIGGKSNSGEGGEDPIRW-NPLTDVVDGYSPTLPHLKGLQNGDTATSAIKQVASGRFGVTPTFLVNADQIEIKIAQGAKPGEGGQLPGKKVSAYIARLRNSKPGVPLISPPPHHDIYSIEDLAQLIYDLHQINPKAKVSVKLVSEAGIGTVASGVSKANADIIQISGHDGGTGASPISSIKHAGGPWELGLTETNQTLIQNGLRERVVLRVDGGFRSGQDVLIAAAMGADEYGFGSVAM-IATGCVMARICHTNNCPVGVASQREELRARFPGVPGDLVNYFLFVAEEVRAALA-------QLGYEKLDDIIGRTDLLKPKHISLV--------KTQHIDLGYLLSNAGLPEWSSSQI---RSQDVHTNGPVLDETILADPEIADAIENEK-EVSKAFQIYNVDRAVCGRVAGVIAKKYGDTGF--AGQLNITFNGSAGQSFGCFLTPGMNIRLVGEANDYVGKGMAGGELVVVPVDKT--GFVPEDATIVGNTCLYGATGGQVFVRGKAGERFAVRNSLCQAVVEGTGDHCCEYMTGGCVVVLGKAGRNVAAGMTGGLAYILDEDDTLVPKVNKEIVKMQRVNAPAGQMQLKGLIEAYVEKTGSEKGIAILREWEAYLPLFWQLVPPSEEDSPEACAEFERVLAKQ--ATTQLSA 1615
BLAST of glutamate synthase vs. nr
Match: gi|1325301526|ref|XP_023332308.1| (glutamate synthase 1 [NADH], chloroplastic-like [Eurytemora affinis] >gi|1325301528|ref|XP_023332309.1| glutamate synthase 1 [NADH], chloroplastic-like [Eurytemora affinis]) HSP 1 Score: 2810.4 bits (7284), Expect = 0.000e+0 Identity = 1404/2180 (64.40%), Postives = 1660/2180 (76.15%), Query Frame = 0
Query: 24 MPFDPDRPRYPEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGK-LGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVTLGDMRKANKHEISTERRPIIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITY-EVSNALNLEDALDKICKEAEKAAREHQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVA-------ALLGADEFG--FSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRAL-------------------------------LDQKAASEQTSSNSIE------------EP----------PKEINGRVTPEIRVTN-------DKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGDTLLPGLGGVIHPRREL 2132
M FD + P +PEK GLYDP+LE+DACGVGFIVHIDG+ SH +L DA++LS RM HRGAC ADNDSGDGAG LVA+P F++ ++ + LG LP +YATGILFL P + Q S+DEF LA TL ++VL WR +P+ N CLGA+AKK+EP++ QVF+V D+ KE+ F FVLRK A+HR ++RFY+ SLS+ T+VYKGQF+PCQL++YFLDL +P+FKT +CIVHTRFSTNTFPSWERAHP R +AHNGEINTLRGN+NLMKAREGVM++ +FG +L L+PVVE + SDSG+LD LEFLV AGNRSLPEAVMT+VPEAWQNDP MD ++KNFY +ASC+ EPWDGPAL+TFSDGRYVGAILDRNGLRPSR+YVT+DNIMVMASEVGV+DS P KILQKGRLMPG+MLLVDT EK+II DE +K +IA SRPH WWNQH+TL + K K E + PI++ + ++ +I+ R+W GDRRMPMFGYT+ETIQMLMVPMF T+KEALGSMGND PLACLS+ QP IYEYFKQLFAQVTNPPIDPFRE+IVMSL CP+GPEGN+LEP C RLWL+NPILSL DL+ +K T V+GWK V+DIT+ + + + E +D+IC+E E+AA QFIILSDRNIG R P+ A LASGA HHHLI+ RLRSKCA+I+ETGE REVH +CV++G+GVDAICPYMVFEIA MLR E +I +IT+ V Y NYAAAVDRGISKVMAKMGIST+QSYK AQIFEAVGLS EV+D+ F GT SR+GG TF I+ EE+L RH++AFGENECDSR+LR+PGF H+RSGGEKH+NDP+S+ALLQEATK+ENK++Y+KY +AA+ SI+DCT+RGQLE+ FPE IDIDEVEPA+ IVKRFATGAMSFGSIS EAH+TLA+AMN++GGKSNTGEGGE+ +RYLNQD ++N RSAIKQVAS RFGVT++YL++ADELQIKMAQGAKPGEGGELPG+KVT +IAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKC NP+ARIS KLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGL+ETHQ LT+NNLRSR + Q A ++ +V VG + G S LI + G + + L+ + + R + + F ++A A LG F + S K +L F+ IL NA +R G SI GGSV+QDF+LE R D +I D L+G T+ I EI N QR FGATLS++I+ RY + GLP+G I+I+L GSAGQSFC FL +G+ VTLEGDANDYVGK LSGG +I+FP KN FKSE+N+I GNVCLYGATSG+A+ RG+ AERFCVRNSGA+A++EGVGDHGCEYMTGGR +ILG TGRNFAAGMSGGIAYVLD +F+ KCN MVDL+ + + D+ FLK L++FV TGS V ++L +W + + F+KVFP EYQRAL LD + A E ++ +I EP K+I+ V P + V + E+ P+ DIED++ D KK+E +LDKTRGFVKYKRE K YRDA +RQ DW EIYDFKHVR+GLK QAARCMDCGVPFCHS SHGCPLGNIIPKFNDLVFH +WKEAL+QL QTNNFPEFTGRVCPAPCEGACVLGINEP VTIKNIEC+IIDNAFE+GWIK PK R+GK V I+GSGPAGLAAADQLNKAGH VTV ER NRIGGLL YGIPTMKLSK+VVQRRVDLMA EGI+FK NVDVGKDI D+KN DAVLL GATWPRDLPI R ++GIHFAMEFLQTWQQ QHGD +D ++A DKDV+VIGGGDTGCDCIGTSLRQGAKSIT+FEILP PP++R DNPWPQ+PRLFKVDYGHEEV++KWG GDPRRYNT S +FL DE +++GI T+ VEW KD+AGRW V GSEK Y CQ+ +LAMGFLGPE +++ L++DPR NI T G Y+T GVFAAGDCRRGQSLVVW ITEGRQAAREVD +L G + LPG G+I P L
Sbjct: 1 MGFDENSPYFPEKQGLYDPALERDACGVGFIVHIDGSSSHQILLDAQELSRRMTHRGACGADNDSGDGAGALVALPHPFYKTKLSEELGIHLPEPDEYATGILFLDPVHHQISRDEFAELAGTLGVRVLIWRELPRSNACLGAMAKKTEPLITQVFLVPSTSDMDKEE--FAKKIFVLRKKATHRF----------TERRFYICSLSTKTVVYKGQFDPCQLWEYFLDLQNPEFKTKLCIVHTRFSTNTFPSWERAHPMRYVAHNGEINTLRGNVNLMKAREGVMSSSRFGDELKDLYPVVEQNLSDSGSLDCCLEFLVQAGNRSLPEAVMTMVPEAWQNDPAMDSSRKNFYEWASCTMEPWDGPALVTFSDGRYVGAILDRNGLRPSRFYVTSDNIMVMASEVGVFDSPPEKILQKGRLMPGKMLLVDTEEKRIIGDEEVKSKIADSRPHGEWWNQHITLDMLSKGFKQE-TLFYNPIVRDMN-GDEPNILERLWTGDRRMPMFGYTIETIQMLMVPMFKTRKEALGSMGNDAPLACLSRYQPQIYEYFKQLFAQVTNPPIDPFREKIVMSLACPVGPEGNILEPGAEQCHRLWLENPILSLGDLQSIKTTNVMGWKAAVVDITFSRLCSCMATE--IDRICEEVEQAAHSCQFIILSDRNIGPDRAPVPALLASGAAHHHLISKRLRSKCALIVETGEAREVHHMCVLLGYGVDAICPYMVFEIAHMLRNEGLIDPEITDHVVYENYAAAVDRGISKVMAKMGISTLQSYKGAQIFEAVGLSQEVIDKSFLGTPSRLGGATFDIIMEETLQRHEVAFGENECDSRILRNPGFFHWRSGGEKHVNDPSSIALLQEATKHENKSAYEKYCEAAMNSIKDCTLRGQLEVVFPE---TGIDIDEVEPAANIVKRFATGAMSFGSISLEAHTTLAIAMNRMGGKSNTGEGGEDADRYLNQDAEFNKRSAIKQVASARFGVTSAYLSHADELQIKMAQGAKPGEGGELPGYKVTKEIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCANPSARISTKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLAETHQTLTLNNLRSRVVLQ-----------ADGQMRTGFDVLVGALLGADEFGFSTAPLI--------------VMGCTMMRKCHLNTCPVGVATQDPELRRKFEGKPEHVVNFFFMIAEELRGYMARLGCKTFQDLIGKTQFLRKRSGLPKKAGMLQFDAILKNALEMRSGISIQGGSVKQDFELEKRSDNILIEKASDVLNGTAKTVDIAMEITNYQRTFGATLSNKISLRYGEEGLPDG-SINIRLTGSAGQSFCAFLARGVSVTLEGDANDYVGKCLSGGTIIVFPHKNGAPEFKSEKNVIVGNVCLYGATSGRAYFRGIGAERFCVRNSGAIAVIEGVGDHGCEYMTGGRTVILGPTGRNFAAGMSGGIAYVLDDLESFRKKCNTSMVDLIGVSNPIDLDFLKSTLEDFVSCTGSEVGSSLLASWPESAKRFIKVFPHEYQRALQELENERAEEEAEKKFSPGIMLNGDHLKSPSLDIRNAYEIDNTPAINGISHLILPAEKMEPLSEDEDEDEDDKKISNFVLPTLTVPKKLPVKVLEIEKKPIRDIEDSIIDSTK-KKKRENLLDKTRGFVKYKRETKNYRDAAERQNDWNEIYDFKHVRRGLKMQAARCMDCGVPFCHSVSHGCPLGNIIPKFNDLVFHSDWKEALNQLTQTNNFPEFTGRVCPAPCEGACVLGINEPPVTIKNIECSIIDNAFEQGWIKACAPKHRTGKKVVIIGSGPAGLAAADQLNKAGHFVTVLERKNRIGGLLMYGIPTMKLSKEVVQRRVDLMASEGIVFKTNVDVGKDIPAADLKNQYDAVLLCTGATWPRDLPIEGRSSDGIHFAMEFLQTWQQKQHGDDIDHLPLSAKDKDVVVIGGGDTGCDCIGTSLRQGAKSITTFEILPQPPNSRGADNPWPQYPRLFKVDYGHEEVALKWG-GDPRRYNTVSKKFLSDEMGRVSGIETMLVEWTKDEAGRWKMAEVPGSEKTYYCQMVMLAMGFLGPEKEILEQMSLDKDPRGNIKTPVGKYSTNVEGVFAAGDCRRGQSLVVWGITEGRQAAREVDIYLTGKSSLPGPAGIIIPPTNL 2133
BLAST of glutamate synthase vs. nr
Match: gi|1067078482|ref|XP_018022904.1| (PREDICTED: putative glutamate synthase [NADPH] [Hyalella azteca]) HSP 1 Score: 2581.21 bits (6689), Expect = 0.000e+0 Identity = 1293/2111 (61.25%), Postives = 1580/2111 (74.85%), Query Frame = 0
Query: 33 YPEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVTLGDMRKANKHEIST--ERRPIIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNA-LNLEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVA-------ALLGADEFG--FSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRAL--LDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAK-KQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLV-GDTLLPGLGGVI 2126
YPEK GLYDPS E ACGVGF+V+IDG S ++RDAE+L++RM+HRGAC DND+GDGAGV+VA+P F+ + + LP G+YATGI FL ++ ES+ +FE LA LKVL WRRVP ++ LG +AK +EP+ QVFV+ PD L +D F +VLRK ASHRL +RFYV SLS+TT+VYKGQ Q++ YFLDL DF+T++ +VHTRFSTNTFPSWERAHP R++AHNGEINTLRGN+NLM AREGVM + FG L L+PVVE + SDSGA+D LEFLVMAG RSLPEA+MT+VPEAWQNDP M K++FYR+A+C+ EPWDGPAL+TF+DGRY+GAILDRNGLRPSRYYVT D ++VMASEVGVYD++P IL KGRL PGRMLLVDT EK + +DE +K +IA SRPH W + T+ D+ ++ + T + P+ V R + GDRR+P FGYTLETI ML++PM T+KEALGSMGND PLACLS+ QPL+Y+YFKQLFAQVTNPPIDPFRERIVMSL CPIGP+ N+LEP C RL+L+NPILSL DL+VLK T W +++IDIT+ S+ L+ A+ ++C EA AA+E HQ ++LSDR +G R+ +SA +A GA HHHLI R R K ++LETGE REVH IC++VG+G DA+CPY+VFE LREE V+ D+ + Y NY+AA+DRG+SKVMAKMGIST+QSYK AQIFEAVG+S E++D CF GTASR+GGVT A++AEE RH LA+GE ECD+ LR+PGFLH+R+GGEKH+NDP SVA LQEA++ + Y K+++ ++SIR+CT+RGQLE+N + IDI EVEPAS IVKRF TGAMSFGSIS EAHSTLA+AMN++G KSNTGEGGE+PERY NQDP+ N RSAIKQVASGRFGVTA+YLANADELQIKMAQGAKPGEGGELPG+KV+ IAKTRHSVPGVGLISPPPHHDIYSIEDLA+LIYDLKC+NP ARISVKLVSEVGVGVVASGVAKGK+EHI ISGHDGGTGASSWTGIK AGLPWELG+SETHQ L +NNLRSR + Q ++C G VV + I G D +++ + G + + L+ + + R + + + L+A A LG +F L+++ + KV+ ++F IL NA LRPG +I+GGSV QDF+L+ LD +I ++G ++ I+ +I+NE RA GATLS+ IA +Y D+GLPEG I+I +KGSAGQSFC FL +G+ VTLEGD+NDYVGKGLSGGEV+IFP K+ + FKS+ N+I GNVCLYGATSG+AF RG+AAERF VRNSGA+A+VEGVGDHGCEYMTGG V+ILG TGRNFAAGMSGGIAYV D N + KCN MV+LLP+ +ED+ ++ L+ FV+KTGSIVA++IL +W + FVKVFP EYQRAL + ++ A ++ ++EP K E V DIED + D + K K E ILDKTRGF+KYKR+ K YRDA+ RQKDW EIY+ ++VRKGLK QAARCMDCGVPFC S SHGCPLGNIIPK+NDL+F+DNWKEAL QLLQTNNFPEFTGRVCPAPCEG+CVLGINE VTIKNIE +IID+AFE+GWIKP P R+GK VA+VGSGPAGLAAA QLNKAGH VTV ERN+RIGGLLQYGIPTMKLSK+VVQRRV L+A EG+ FK N VGKDI+ ++++++DAV+L +GATWPRDLPI R GI FAM FL+TWQ+ Q G+ +D ++TA DK+VIVIGGGDTGCDCI TSLRQGAKSIT+FEILP PP +R DNPWP +PR+FKVDYGHEEV +K+G+ DPR+Y+T + EFL D A + G++TVQVEW KDDAGRW + G+EK+Y+ L LLAMGFLGPE +I L+QDPRSNI T G YAT + VFAAGDCRRGQSLVVWAI+EGRQAAREVD FL+ G + L +GG++
Sbjct: 7 YPEKQGLYDPSYETAACGVGFVVNIDGESSPKIIRDAERLASRMKHRGACGCDNDTGDGAGVMVAMPHSFYRSILKEADLELPAPGRYATGIFFLEESSVVESQGQFENLATAHGLKVLHWRRVPVDSSVLGKVAKSTEPLQMQVFVI--PPDDL-DDHAFHRQVWVLRKRASHRLP--------APGRRFYVCSLSTTTMVYKGQLTSDQIWTYFLDLASADFQTYLALVHTRFSTNTFPSWERAHPLRMMAHNGEINTLRGNVNLMTAREGVMKSAVFGKDLPTLYPVVEPNLSDSGAVDCCLEFLVMAGGRSLPEAIMTMVPEAWQNDPNMHPEKRDFYRWAACAMEPWDGPALMTFTDGRYIGAILDRNGLRPSRYYVTNDRVLVMASEVGVYDTEPSNILTKGRLKPGRMLLVDTFEKIVSKDEELKLQIARSRPHAEWIKEQFTMADLYAVHEGSLPTMEHQTPVTSPTTSSPVGEGVTRTYGGDRRLPAFGYTLETISMLLLPMVKTEKEALGSMGNDAPLACLSEYQPLMYDYFKQLFAQVTNPPIDPFRERIVMSLACPIGPQANILEPCAEQCHRLFLENPILSLNDLQVLKATRHRNWCSQIIDITFPASDGPAGLQAAIQRVCDEACVAAKEGHQILVLSDRAMGPDRVAVSALMALGATHHHLIEKRQRMKVGLVLETGEAREVHHICLLVGYGADAVCPYLVFETMASLREEGVLPQDLDDHTIYKNYSAAMDRGLSKVMAKMGISTLQSYKGAQIFEAVGISAEIIDTCFKGTASRLGGVTMAMVAEEQFQRHLLAYGERECDTLTLRNPGFLHWRAGGEKHMNDPDSVAHLQEASRSNSNNEYDKFLEHTMKSIRECTLRGQLELN---KIAAPIDISEVEPASGIVKRFVTGAMSFGSISMEAHSTLAIAMNRVGAKSNTGEGGEDPERYTNQDPNNNRRSAIKQVASGRFGVTAAYLANADELQIKMAQGAKPGEGGELPGYKVSDGIAKTRHSVPGVGLISPPPHHDIYSIEDLAELIYDLKCSNPNARISVKLVSEVGVGVVASGVAKGKAEHITISGHDGGTGASSWTGIKNAGLPWELGISETHQTLVLNNLRSRVVLQADGQIRC---------------GFDVVVAA----------ILGADEFGFSTAPLIVMGCTMMRKCHLNTCPVGVATQDPELRKKFEGKPEHVVNYLFLLAEEVRKHMASLGIRKFQDLIGRTDLLSVRKSSMAKVNTVDFSAILKNALTLRPGTNIVGGSVAQDFELDKHLDNILIERAAGVIEGTEKSVDIKLDIHNECRAVGATLSYRIALKYGDAGLPEGSSINISMKGSAGQSFCNFLSRGVHVTLEGDSNDYVGKGLSGGEVVIFPEKDLPENFKSDANVIVGNVCLYGATSGQAFFRGIAAERFAVRNSGAVAVVEGVGDHGCEYMTGGVVVILGITGRNFAAGMSGGIAYVYDK--NLEKKCNTSMVELLPLEKEEDLAYVANLLKLFVEKTGSIVAEDILSDWPAKASNFVKVFPYEYQRALRQIAEREALKEEEKLPVKEPA----------------KNEPVVQDIEDTIADSQQMDKSKAERILDKTRGFMKYKRQTKTYRDADARQKDWDEIYNHENVRKGLKTQAARCMDCGVPFCQS-SHGCPLGNIIPKWNDLIFNDNWKEALQQLLQTNNFPEFTGRVCPAPCEGSCVLGINELPVTIKNIEVSIIDHAFEQGWIKPEVPLSRTGKKVAVVGSGPAGLAAAHQLNKAGHSVTVLERNDRIGGLLQYGIPTMKLSKEVVQRRVQLLAAEGVEFKTNAYVGKDISAKELRDSHDAVVLCMGATWPRDLPIAGRSLNGIEFAMTFLETWQKKQMGNVLDTERVTARDKNVIVIGGGDTGCDCIATSLRQGAKSITTFEILPQPPPSRGRDNPWPTYPRVFKVDYGHEEVQLKFGR-DPRQYSTLTKEFLDDGAGNVKGVKTVQVEWTKDDAGRWKMDEIAGTEKVYDADLILLAMGFLGPEKDIIGELELDQDPRSNIKTPAGKYATSVSKVFAAGDCRRGQSLVVWAISEGRQAAREVDAFLMQGRSSLSTVGGLV 2058
BLAST of glutamate synthase vs. nr
Match: gi|817077916|ref|XP_012261308.1| (putative glutamate synthase [NADPH] isoform X2 [Athalia rosae]) HSP 1 Score: 2551.93 bits (6613), Expect = 0.000e+0 Identity = 1297/2112 (61.41%), Postives = 1566/2112 (74.15%), Query Frame = 0
Query: 34 PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVG-KLGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVTLGDMRKAN-----KHEISTERRPIIKSLALLNDH----SIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNAL-NLEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVG---VVASGVAKGKSEHILISGHDGGTGASSWTGI---KGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLS-KITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGDTLLPGLGGVI 2126
P K GLYDP+LEKDACGVGFIV IDG +SH ++RDAE+LS RM HRGAC+ DNDSGDGAGVL AIP ++ E+ + G LP G YATGI FL Q+++ F LA +L+++ WR VP ++ +G +A+KSEP + QVFV + E FVLRK ASH + Q RFY+ SLS T+VYKGQF QL++YF DL P+F+T++ +VHTRFSTNTFPSWERAHP R+LAHNGEINTLRGN+NLMKAREGVM++ +G +L QL+PVVE + SDSGA D VLEFLVMAG R+LPEAVMT+VPEAWQND M K+++Y +A+C+ EPWDGPALLTF+DGRYVGAILDRNGLRPSR+YVT DN+MVMASEVGVYD+ P ++ K RL PGRMLLVDT EK+IIQD +K +IA SRPH W + +++ +R+A+ + I+ + L ND S V R+W GD+R+ ++GYT+ETI ML++PM TKKEALGSMGND PLACLS+ QPLIYEYFKQLFAQVTNPPIDPFRE+IVMSL CPIGP N+LEPNE RL+L PILSL DLEV+K T GW+TKVIDITY + L LD++C EA +AARE +Q ++LSDR G R+P+S LA GAVHH LI R R + +ILET E REVH +CV++G+G DAICPY+VFE+A LR + V+ T++V Y NY A++RG++KVMAKMGIST+QSYK AQIFEAVGL+++VVD+CF GT SRIGGVTF I+A+E+ RH++ + E D VLR+PG H+R+GGEKHINDP S+A LQEA + ++ +Y+ Y + +E++R CT+RGQL++ P DK I IDE+EPA+EIVKRF TGAMSFGSIS EAH+TLA+AMN++GGKSNTGEGGEN +RYL QDP ++ RSAIKQVASGRFGVT+SYLANAD+LQIKMAQGAKPGEGGELPG+KVT DIA TRHSVPGVGLISPPPHHDIYSIEDLA+LIYDLKC NP ARISVKLVSEVGVGVVASGVAKGK+EHI+ISGHDGGTGASSWTGIK AGLPWELG++ETHQVLT+NNLRSR + Q L+ ++ E G ++A G + H+ + G ++ + K AG P L M R + + G +R D L+G +F + + ++ K LNF IL +A LRPG +I GGSV+QDFQLE RLD ++I L G ++ IE INNE RAF +TLS+ I+K++ + GLPE I+I + GSAGQSFC F+ KG+ VTLEGDANDYVGKGL GGE++I+PPK+S F SE N+I GNVCLYGATSG+A+ RG+AAERF VRNSGA+ +VEGVGDHGCEYMTGG +ILG TGRNFAAGMSGGIAYVLD +G+FK KCN EMV+LLP+ +DI ++KE L+EFV+KTGS++A ++LK W FVKVFP EYQRAL K +E+ S I +NG N E V DIEDA+ D DM +K LDK RGFVKY RE K YR A R +DW EIY+F+ VRKGL+ QAARCM+CGVPFC S SHGCPLGNIIPK+NDLVFH NWKEAL+QLLQTNNFPEFTGRVCPAPCEGACVLGI+EPAVTIKNIECAIID+AFE+GWI PP R+G+ VAIVGSGP+GLAAA QLNKAGHLVTVYERN+R+GGLLQYGIPTMKLSK VVQRR+ L+A EGI FK V+VGKDI+ +++ DA+LL GATWPRDLPIP R+ EGIHFAM FL+ WQ+ Q G+ K+ A DKDVI+IGGGDTGCDCI TSLRQGAKSIT+FEILP PP R DNPWPQ+PR+FKVDYGHEEV++K+G+ DPR+++T S EFL D+ ++GI+TV VEW KD+AGRW V SEKIY+C L LLAMGFLGPE YV + D R N T G ++T GVFAAGDCRRGQSLVVWAI EGRQAAREVD L+G T LP GGVI
Sbjct: 10 PVKQGLYDPTLEKDACGVGFIVAIDGKRSHKIVRDAEKLSARMNHRGACACDNDSGDGAGVLCAIPHNYYATELREQQGIELPEFGHYATGIFFLDKATHQQTEAAFTKLAEECNLRIISWRDVPTDSGTIGQVARKSEPYIRQVFVTGDQ-----ETEELNRQIFVLRKRASHTIP--------QPGVRFYICSLSLKTVVYKGQFTADQLWKYFTDLSSPNFETYLTLVHTRFSTNTFPSWERAHPLRLLAHNGEINTLRGNVNLMKAREGVMSSSIYGDKLKQLYPVVEPNLSDSGAADCVLEFLVMAGQRTLPEAVMTMVPEAWQNDLTMATEKRDYYHWAACAMEPWDGPALLTFTDGRYVGAILDRNGLRPSRFYVTKDNMMVMASEVGVYDTPPSNVVLKSRLKPGRMLLVDTEEKQIIQDVDLKIQIARSRPHSTWLKEQISMDQLREAHVAVHGSNGITDNGGVELTKLNGTNDKIDGVSAVNRVWGGDKRLSLYGYTIETINMLILPMIQTKKEALGSMGNDAPLACLSEFQPLIYEYFKQLFAQVTNPPIDPFREKIVMSLMCPIGPVSNVLEPNELQVHRLFLSQPILSLKDLEVIKHTNYRGWRTKVIDITYPAEDGPPGLMKTLDRVCNEANEAAREGYQLLVLSDRRAGPERVPVSTLLAQGAVHHFLIEERQRMRVGLILETAEAREVHHMCVLLGYGADAICPYLVFEMARSLRADGVLEESFTDDVMYQNYCEAMERGVAKVMAKMGISTLQSYKGAQIFEAVGLADDVVDKCFKGTQSRIGGVTFDIIAKEAFERHQMTYMEKAVDMMVLRNPGIYHWRAGGEKHINDPNSIANLQEAVESKSNNAYENYRKSTMEAVRACTLRGQLDLK-PIDKP--IPIDEIEPAAEIVKRFVTGAMSFGSISLEAHTTLAIAMNRIGGKSNTGEGGENADRYLGQDPQFSKRSAIKQVASGRFGVTSSYLANADDLQIKMAQGAKPGEGGELPGYKVTADIAATRHSVPGVGLISPPPHHDIYSIEDLAELIYDLKCANPEARISVKLVSEVGVGVVASGVAKGKAEHIVISGHDGGTGASSWTGIKAAGLPWELGVAETHQVLTLNNLRSRVVVQADGQLRTGFDVVVAALLGADEFGFSTAPLIAMGCTMMRKCHL----NTCPVGIATQDPVLRKKFAGKP-----EHVVNFLFMLAEEVRTHMASLG-IRKFQD-----LVGRTDF-------LRIAGSNHEKAKTLNFANILRSALELRPGINIRGGSVKQDFQLENRLDNKLIEEATPILQGLKKSVNIEMAINNECRAFASTLSYYISKKFGEEGLPEH-SININMTGSAGQSFCAFMTKGVHVTLEGDANDYVGKGLCGGEIVIYPPKDSD--FNSEANVIVGNVCLYGATSGRAYFRGIAAERFSVRNSGAIVVVEGVGDHGCEYMTGGCAVILGLTGRNFAAGMSGGIAYVLDVDGSFKSKCNPEMVELLPLNQQDDIAYVKELLEEFVEKTGSLIAQDLLKVWPEPTTRFVKVFPYEYQRAL---KQLAEKKESQLI------LNG--------NNKSSEPSVQDIEDAIADGDMEKRK----LDKIRGFVKYGRETKNYRPAAKRLEDWDEIYNFQGVRKGLRVQAARCMECGVPFCQS-SHGCPLGNIIPKWNDLVFHSNWKEALNQLLQTNNFPEFTGRVCPAPCEGACVLGISEPAVTIKNIECAIIDHAFEQGWITAQPPNTRTGRKVAIVGSGPSGLAAAHQLNKAGHLVTVYERNDRVGGLLQYGIPTMKLSKQVVQRRISLLAAEGITFKTGVNVGKDISAKELREQYDALLLCTGATWPRDLPIPGRQFEGIHFAMSFLENWQKKQMGNTAPPQMKLIAKDKDVIIIGGGDTGCDCIATSLRQGAKSITTFEILPEPPVKRGKDNPWPQYPRVFKVDYGHEEVALKFGR-DPRQFSTLSKEFLGDDKGNVSGIKTVTVEWTKDEAGRWKMDEVPNSEKIYKCDLVLLAMGFLGPEKYVATELEAKLDGRGNYETPGGKFSTSLPGVFAAGDCRRGQSLVVWAIAEGRQAAREVDLALMGCTTLPSPGGVI 2057
BLAST of glutamate synthase vs. nr
Match: gi|817077910|ref|XP_012261305.1| (putative glutamate synthase [NADPH] isoform X1 [Athalia rosae] >gi|817077912|ref|XP_012261306.1| putative glutamate synthase [NADPH] isoform X1 [Athalia rosae] >gi|817077914|ref|XP_012261307.1| putative glutamate synthase [NADPH] isoform X1 [Athalia rosae] >gi|1183228545|ref|XP_020709785.1| putative glutamate synthase [NADPH] isoform X1 [Athalia rosae]) HSP 1 Score: 2548.47 bits (6604), Expect = 0.000e+0 Identity = 1298/2113 (61.43%), Postives = 1566/2113 (74.11%), Query Frame = 0
Query: 34 PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVG-KLGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWN-QHVTLGDMRKAN-----KHEISTERRPIIKSLALLNDH----SIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNAL-NLEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVG---VVASGVAKGKSEHILISGHDGGTGASSWTGI---KGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLS-KITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGDTLLPGLGGVI 2126
P K GLYDP+LEKDACGVGFIV IDG +SH ++RDAE+LS RM HRGAC+ DNDSGDGAGVL AIP ++ E+ + G LP G YATGI FL Q+++ F LA +L+++ WR VP ++ +G +A+KSEP + QVFV + E FVLRK ASH + Q RFY+ SLS T+VYKGQF QL++YF DL P+F+T++ +VHTRFSTNTFPSWERAHP R+LAHNGEINTLRGN+NLMKAREGVM++ +G +L QL+PVVE + SDSGA D VLEFLVMAG R+LPEAVMT+VPEAWQND M K+++Y +A+C+ EPWDGPALLTF+DGRYVGAILDRNGLRPSR+YVT DN+MVMASEVGVYD+ P ++ K RL PGRMLLVDT EK+IIQD +K +IA SRPH W Q +++ +R+A+ + I+ + L ND S V R+W GD+R+ ++GYT+ETI ML++PM TKKEALGSMGND PLACLS+ QPLIYEYFKQLFAQVTNPPIDPFRE+IVMSL CPIGP N+LEPNE RL+L PILSL DLEV+K T GW+TKVIDITY + L LD++C EA +AARE +Q ++LSDR G R+P+S LA GAVHH LI R R + +ILET E REVH +CV++G+G DAICPY+VFE+A LR + V+ T++V Y NY A++RG++KVMAKMGIST+QSYK AQIFEAVGL+++VVD+CF GT SRIGGVTF I+A+E+ RH++ + E D VLR+PG H+R+GGEKHINDP S+A LQEA + ++ +Y+ Y + +E++R CT+RGQL++ P DK I IDE+EPA+EIVKRF TGAMSFGSIS EAH+TLA+AMN++GGKSNTGEGGEN +RYL QDP ++ RSAIKQVASGRFGVT+SYLANAD+LQIKMAQGAKPGEGGELPG+KVT DIA TRHSVPGVGLISPPPHHDIYSIEDLA+LIYDLKC NP ARISVKLVSEVGVGVVASGVAKGK+EHI+ISGHDGGTGASSWTGIK AGLPWELG++ETHQVLT+NNLRSR + Q L+ ++ E G ++A G + H+ + G ++ + K AG P L M R + + G +R D L+G +F + + ++ K LNF IL +A LRPG +I GGSV+QDFQLE RLD ++I L G ++ IE INNE RAF +TLS+ I+K++ + GLPE I+I + GSAGQSFC F+ KG+ VTLEGDANDYVGKGL GGE++I+PPK+S F SE N+I GNVCLYGATSG+A+ RG+AAERF VRNSGA+ +VEGVGDHGCEYMTGG +ILG TGRNFAAGMSGGIAYVLD +G+FK KCN EMV+LLP+ +DI ++KE L+EFV+KTGS++A ++LK W FVKVFP EYQRAL K +E+ S I +NG N E V DIEDA+ D DM +K LDK RGFVKY RE K YR A R +DW EIY+F+ VRKGL+ QAARCM+CGVPFC S SHGCPLGNIIPK+NDLVFH NWKEAL+QLLQTNNFPEFTGRVCPAPCEGACVLGI+EPAVTIKNIECAIID+AFE+GWI PP R+G+ VAIVGSGP+GLAAA QLNKAGHLVTVYERN+R+GGLLQYGIPTMKLSK VVQRR+ L+A EGI FK V+VGKDI+ +++ DA+LL GATWPRDLPIP R+ EGIHFAM FL+ WQ+ Q G+ K+ A DKDVI+IGGGDTGCDCI TSLRQGAKSIT+FEILP PP R DNPWPQ+PR+FKVDYGHEEV++K+G+ DPR+++T S EFL D+ ++GI+TV VEW KD+AGRW V SEKIY+C L LLAMGFLGPE YV + D R N T G ++T GVFAAGDCRRGQSLVVWAI EGRQAAREVD L+G T LP GGVI
Sbjct: 10 PVKQGLYDPTLEKDACGVGFIVAIDGKRSHKIVRDAEKLSARMNHRGACACDNDSGDGAGVLCAIPHNYYATELREQQGIELPEFGHYATGIFFLDKATHQQTEAAFTKLAEECNLRIISWRDVPTDSGTIGQVARKSEPYIRQVFVTGDQ-----ETEELNRQIFVLRKRASHTIP--------QPGVRFYICSLSLKTVVYKGQFTADQLWKYFTDLSSPNFETYLTLVHTRFSTNTFPSWERAHPLRLLAHNGEINTLRGNVNLMKAREGVMSSSIYGDKLKQLYPVVEPNLSDSGAADCVLEFLVMAGQRTLPEAVMTMVPEAWQNDLTMATEKRDYYHWAACAMEPWDGPALLTFTDGRYVGAILDRNGLRPSRFYVTKDNMMVMASEVGVYDTPPSNVVLKSRLKPGRMLLVDTEEKQIIQDVDLKIQIARSRPHSTWLKEQRISMDQLREAHVAVHGSNGITDNGGVELTKLNGTNDKIDGVSAVNRVWGGDKRLSLYGYTIETINMLILPMIQTKKEALGSMGNDAPLACLSEFQPLIYEYFKQLFAQVTNPPIDPFREKIVMSLMCPIGPVSNVLEPNELQVHRLFLSQPILSLKDLEVIKHTNYRGWRTKVIDITYPAEDGPPGLMKTLDRVCNEANEAAREGYQLLVLSDRRAGPERVPVSTLLAQGAVHHFLIEERQRMRVGLILETAEAREVHHMCVLLGYGADAICPYLVFEMARSLRADGVLEESFTDDVMYQNYCEAMERGVAKVMAKMGISTLQSYKGAQIFEAVGLADDVVDKCFKGTQSRIGGVTFDIIAKEAFERHQMTYMEKAVDMMVLRNPGIYHWRAGGEKHINDPNSIANLQEAVESKSNNAYENYRKSTMEAVRACTLRGQLDLK-PIDKP--IPIDEIEPAAEIVKRFVTGAMSFGSISLEAHTTLAIAMNRIGGKSNTGEGGENADRYLGQDPQFSKRSAIKQVASGRFGVTSSYLANADDLQIKMAQGAKPGEGGELPGYKVTADIAATRHSVPGVGLISPPPHHDIYSIEDLAELIYDLKCANPEARISVKLVSEVGVGVVASGVAKGKAEHIVISGHDGGTGASSWTGIKAAGLPWELGVAETHQVLTLNNLRSRVVVQADGQLRTGFDVVVAALLGADEFGFSTAPLIAMGCTMMRKCHL----NTCPVGIATQDPVLRKKFAGKP-----EHVVNFLFMLAEEVRTHMASLG-IRKFQD-----LVGRTDF-------LRIAGSNHEKAKTLNFANILRSALELRPGINIRGGSVKQDFQLENRLDNKLIEEATPILQGLKKSVNIEMAINNECRAFASTLSYYISKKFGEEGLPEH-SININMTGSAGQSFCAFMTKGVHVTLEGDANDYVGKGLCGGEIVIYPPKDSD--FNSEANVIVGNVCLYGATSGRAYFRGIAAERFSVRNSGAIVVVEGVGDHGCEYMTGGCAVILGLTGRNFAAGMSGGIAYVLDVDGSFKSKCNPEMVELLPLNQQDDIAYVKELLEEFVEKTGSLIAQDLLKVWPEPTTRFVKVFPYEYQRAL---KQLAEKKESQLI------LNG--------NNKSSEPSVQDIEDAIADGDMEKRK----LDKIRGFVKYGRETKNYRPAAKRLEDWDEIYNFQGVRKGLRVQAARCMECGVPFCQS-SHGCPLGNIIPKWNDLVFHSNWKEALNQLLQTNNFPEFTGRVCPAPCEGACVLGISEPAVTIKNIECAIIDHAFEQGWITAQPPNTRTGRKVAIVGSGPSGLAAAHQLNKAGHLVTVYERNDRVGGLLQYGIPTMKLSKQVVQRRISLLAAEGITFKTGVNVGKDISAKELREQYDALLLCTGATWPRDLPIPGRQFEGIHFAMSFLENWQKKQMGNTAPPQMKLIAKDKDVIIIGGGDTGCDCIATSLRQGAKSITTFEILPEPPVKRGKDNPWPQYPRVFKVDYGHEEVALKFGR-DPRQFSTLSKEFLGDDKGNVSGIKTVTVEWTKDEAGRWKMDEVPNSEKIYKCDLVLLAMGFLGPEKYVATELEAKLDGRGNYETPGGKFSTSLPGVFAAGDCRRGQSLVVWAIAEGRQAAREVDLALMGCTTLPSPGGVI 2058
BLAST of glutamate synthase vs. nr
Match: gi|998499271|ref|XP_015509518.1| (PREDICTED: putative glutamate synthase [NADPH] isoform X2 [Neodiprion lecontei]) HSP 1 Score: 2548.08 bits (6603), Expect = 0.000e+0 Identity = 1299/2118 (61.33%), Postives = 1565/2118 (73.89%), Query Frame = 0
Query: 34 PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVG-KLGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVV-RKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWN-QHVTLGDMRKAN---------KHEISTERRPIIKSLALLNDHSIVARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVS-NALNLEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVG---VVASGVAKGKSEHILISGHDGGTGASSWTGI---KGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESP-VADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGD----KMDLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGDTLLPGLGGVI 2126
P K GLYDP+LEKDACGVGFIV IDG +SH ++RDAE+LS RM HRGAC+ DNDSGDGAGVL AIP +++ E+ + G LP G YATGI FL Q+S++ F LA +L+++CWR VP +N +G +A K+EP QVFV +E + L FVLRK ASH + Q RFY+ SLS T+VYKGQF QL++Y+ DL PDF+T++ +VHTRFSTNTFPSWERAHP R+LAHNGEINTLRGN+NLMKAREGVM++ +G +L QL+PVVE + SDSGA D VLEFLVMAG R+LPEAVMT+VPEAWQND M K++FY +A+C+ EPWDGPALLTF+DGRYVGAILDRNGLRPSR+YVT DN+MVMASEVGVYD+ P ++ K RL PGRMLLVDT EKKIIQD +K IA SRPH W Q +++ +R+A+ S E +I++ S V R+W GD+R+ ++GYT+ETI ML++PM TKKEALGSMGND PLACLS QPLIYEYFKQLFAQVTNPPIDPFRE+IVMSL CPIGP N+LEPNE RL+L PILSL DLEV+K T GW+TKVIDITY L+ + ++C EA +AA + +Q I+LSDR G R+P+S LA GAVHH LI R R K +ILET E REVH +CV++G+G DAICPY+VFE+A LR + V+ T+++ + NY+ A++RGI+KVMAKMGIST+QSYK AQIFEAVGL+++VVD+CF GT SRIGGVTF I+A+E+ RH++ + E D VLR+PG H+R+GGEKHIN+P S+A LQEA + ++ +Y+ Y + ++++R CT+RGQ+EI + K I IDEVEPASEIVKRF TGAMSFGSIS EAH+TLA+AMN++GGKSNTGEGGEN +RYL+QDP++N RS+IKQVASGRFGVT+SY+ANAD+LQIKMAQGAKPGEGGELPG+KVT DIA TRHSVPGVGLISPPPHHDIYSIEDLA+LIYDLKC NP ARISVKLVSEVGVGVVASGVAKGK+EHI+ISGHDGGTGASSWTGIK AGLPWELG++ETHQVLT+NNLRSR + Q L+ ++ E G ++A G + H+ + G ++ I K AG P L M R + + G +R D L+G +F + + S D K LNF IL NA +RPG +I GGSV+QDFQLE RLD +++ L+G ++ I INNE RAF +TLS+ I+K++ + GLPE I+I + GSAGQSFC F+ KG+ VTLEGDANDYVGK L GGE+II+PPK+S F SE N+I GNVCLYGATSG+A+ RG+AAERF VRNSGA+ +VEGVGDHGCEYMTGG +ILG TGRNFAAGMSGGIAYVLD +G+FK KCN EMV+LLP+ DI ++KE L+EF++KTGS++A +LK W FVKVFP EYQRAL K +EQ +V I +N K P V DIED++ D DM KK LDK RGFVKY RE K YR AE R +DW EIY+F+ VRKGL+ QAARCM+CGVPFC S SHGCPLGNIIPK+NDLVFH NWKEAL+QLLQTNNFPEFTGRVCPAPCEGACVLGI+EP VTIKNIECAIID+AFE+GWI PPK R+G+ VAIVGSGP+GLAAA QLNKAGHLVTVYERN+R+GGLLQYGIPTMKLSK VVQRRV L+A EGI FK ++VGKDI+ ++K DA+LL GATWPRDL IP R EGIHFAM FL+ WQ+ Q G+ KM+L A +KDVI+IGGGDTGCDCI TSLRQGA+SIT+FEILP PP R DNPWPQ+PR+FKVDYGHEEVS+K+G+ DPR+++T S EFL ++ +TGIRTV VEW KD+AGRW V SEK Y+C L LLAMGF+GPE YV + D R N T G Y+T +GVFAAGDCRRGQSLVVWAI EGRQAAREVD+ L+G T LP GGVI
Sbjct: 10 PVKQGLYDPTLEKDACGVGFIVAIDGKRSHKIVRDAEKLSARMNHRGACACDNDSGDGAGVLCAIPHDYYAAEIREQHGVELPEFGHYATGIFFLDKITHQQSEEAFAKLAEECNLRIICWRDVPTDNTKIGQVAYKTEPYSRQVFVTGDQEVEALNR------QVFVLRKRASHTIP--------QPGIRFYICSLSLKTVVYKGQFTADQLWKYYTDLSSPDFETYLALVHTRFSTNTFPSWERAHPLRLLAHNGEINTLRGNVNLMKAREGVMSSPIYGDKLKQLYPVVEPNLSDSGAADCVLEFLVMAGQRTLPEAVMTMVPEAWQNDLTMATEKRDFYHWAACAMEPWDGPALLTFTDGRYVGAILDRNGLRPSRFYVTKDNMMVMASEVGVYDTPPSNVVLKSRLKPGRMLLVDTEEKKIIQDVDLKIYIARSRPHSTWLKEQRISMDQLREAHIAVNGSNGITENGSVELTKLIETNDTFEGVSAVNRVWGGDKRLSLYGYTIETINMLLLPMIQTKKEALGSMGNDAPLACLSDFQPLIYEYFKQLFAQVTNPPIDPFREKIVMSLMCPIGPVSNILEPNELQVHRLFLHQPILSLKDLEVIKNTNYRGWRTKVIDITYPAEYGPPGLQKTIHRVCNEANEAAHQGYQLIVLSDRLAGPDRVPVSTLLAQGAVHHFLIEERQRMKVGLILETAEAREVHHMCVLLGYGADAICPYLVFEMARSLRADGVLDESCTDDIMFTNYSEAMERGIAKVMAKMGISTLQSYKGAQIFEAVGLADDVVDKCFKGTQSRIGGVTFDIIAKEAFERHQMTYMEKAVDMMVLRNPGIYHWRAGGEKHINEPNSIANLQEAVESKSTIAYENYRKSTMDAVRACTLRGQMEI---KTIDKPIPIDEVEPASEIVKRFVTGAMSFGSISLEAHTTLAIAMNRIGGKSNTGEGGENADRYLDQDPEFNKRSSIKQVASGRFGVTSSYIANADDLQIKMAQGAKPGEGGELPGYKVTADIAATRHSVPGVGLISPPPHHDIYSIEDLAELIYDLKCANPNARISVKLVSEVGVGVVASGVAKGKAEHIVISGHDGGTGASSWTGIKAAGLPWELGVAETHQVLTLNNLRSRVVVQADGQLRTGFDVVVAALLGADEFGFSTAPLIAMGCTMMRKCHL----NTCPVGIATQDPILRKKFAGKP-----EHVINFLFMLAEEVRTHMASLG-IRKFQD-----LVGRTDF-------LKVASRDHEKSKTLNFTNILRNALEMRPGVNIKGGSVKQDFQLENRLDNKLLEEAAPVLEGLKKSVSIHMSINNECRAFASTLSYHISKKFGEEGLPEH-SININMTGSAGQSFCAFMTKGVHVTLEGDANDYVGKSLCGGEIIIYPPKDSD--FNSEANVIVGNVCLYGATSGRAYFRGIAAERFSVRNSGAIVVVEGVGDHGCEYMTGGCALILGLTGRNFAAGMSGGIAYVLDVDGSFKSKCNPEMVELLPLNQQSDIAYVKELLEEFIEKTGSLIAQELLKVWPEPTTRFVKVFPYEYQRAL---KQLAEQ---------------KVAQPIVDSNRKPSEPNVKDIEDSIADGDMEKKK----LDKIRGFVKYGRETKNYRPAEKRMEDWNEIYNFQGVRKGLRVQAARCMECGVPFCQS-SHGCPLGNIIPKWNDLVFHSNWKEALNQLLQTNNFPEFTGRVCPAPCEGACVLGISEPPVTIKNIECAIIDHAFEQGWITVQPPKSRTGRRVAIVGSGPSGLAAAHQLNKAGHLVTVYERNDRVGGLLQYGIPTMKLSKQVVQRRVSLLAAEGISFKTGINVGKDISAKELKEQYDALLLCTGATWPRDLQIPGRHFEGIHFAMSFLENWQKKQMGNTVPPKMEL---IAQNKDVIIIGGGDTGCDCIATSLRQGARSITTFEILPEPPLKRGKDNPWPQYPRVFKVDYGHEEVSLKFGR-DPRQFSTLSKEFLGNDKGHVTGIRTVTVEWTKDEAGRWKMDEVPNSEKTYKCDLVLLAMGFMGPEKYVATDLEAKLDGRGNYETPGGKYSTSISGVFAAGDCRRGQSLVVWAIAEGRQAAREVDKSLMGSTTLPSPGGVI 2058
BLAST of glutamate synthase vs. nr
Match: gi|1228004112|ref|XP_021933944.1| (glutamate synthase [NADH], amyloplastic isoform X3 [Zootermopsis nevadensis] >gi|646702782|gb|KDR11809.1| Glutamate synthase [NADH], amyloplastic [Zootermopsis nevadensis]) HSP 1 Score: 2538.84 bits (6579), Expect = 0.000e+0 Identity = 1276/2143 (59.54%), Postives = 1572/2143 (73.36%), Query Frame = 0
Query: 34 PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEV-GKLGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVTLGDMR------KANKHEISTERRPIIKSLALLNDHSI----VARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNALN-LEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKC------------------TNPTARISVKLVSEVGVGVVASGVA----------KGKSEHIL---------ISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGDTLLPGLGGVI 2126
P K GLYDP E+DACGVGFIV IDG +SH ++RDAE+L+ RM HRGAC+ DND+GDGAGVL AIP F+ +E+ + LPP G+YATGI+FL + Q+S+ F LA L+VLCWR VP ++ +G +A+ SEP M QVFV + +D FVLRK A+H++ + RFYV SLS +VYKGQF QL+ YF DL P++ T++ +VHTRFSTNTFPSWERAHP R +AHNGEINTLRGN+NLMKAREGVM + FGS L +L+PVVE + SDSGA+D VLEFLVMAGNR +PEAVMT+VPEAWQND M K++FY +++C+ EPWDGPALLTF+DGRYVGAILDRNGLRPSR+YV DN+MVMASEVGVYD+DP +L K RL PGRMLLVDT E+ +IQD +KK IA SRPH W + + + D+R K N + ++ ++ + + I +A++W GDRR+ +F YT+ETI L++PM TKKEALGSMGND PLACLS PL Y+YFKQLFAQVTNPPIDPFRE++VMSL+CPIGPE NLLEP+E+ C RL L +PILSL DLEV+K T GWKTKVIDIT++ L ALD+IC+EA KAA++ +QFI+LSDR G +RIP+S LA GA HH+LI R R K +ILET E REVH +CV++G+G DA+CPY+VFEI++ LR+E V+ S +T++V Y NY+ A++RGI+KVMAKMGIST+QSYK AQIFEAVGL++EV+++CF GT SRIGGVTF ILA+E+ RH L++G++ CD VLR+PG H+RSGGEKHINDP S+A LQEA ++ +Y ++ ++ +E ++ C +RGQLE+ ++ ID+ VEPASEIVKRFATGAMSFGSIS EAH+TLA+AMNK+GGKSNTGEGGE+ +RYL+QDP++N RSAIKQVASGRFGVT+SYLA+AD+LQIKMAQGAKPGEGGELPG KVT +IA TRHSVPGVGLISPPPHHDIYSIEDLA+LIYDLKC NP ARISVKLVSEVGVGVVASGVAKGK+EHI+ISGHDGGTGASSWTGIK AGLPWELG++ETHQVL +NNLRSR + Q ++ T P + ++ + + G+A +GK EH++ I GH G + + G +G + + T+N ++L+ MR G +++ GGS QDFQLE RLD E+I L G + I +I+NE RAF +TLS+ I+ R D+GLP+ I+++L GSAGQSFC F+ KGI VTLEGDANDYVGKGLSGGEVII+PP++S FKSE N+I GNVCLYGATSGKAF RG+AAERF VRNSGA+A+VEGVGDHGCEYMTGG +ILG TGRNFAAGMSGGIAY+LD + +F+ KCN EMV+L P+ +ED+ ++K L+EF +KTGS++A+++L+ W + FVKVFP EYQRAL + +E+ + ++++ +NG++ ES V DIE++V D K+ + ILDKTRGF+KYKRE MYR AE R KDW+EIY+F HVRKGL+ QAARCM+CGVPFC S SHGCPLGNIIPK+NDLVFH++WKEAL+QLLQTNNFPEFTGRVCPAPCEGACVLGI+EP VTIKNIECAIID+AFE+GWI P P R+ K VA+VGSGP+GLAAA QLNKAGHLVTV+ERN+R+GGLLQYGIPTMKLSK+VVQRRV L+A+EGI FK N++VGKDI+ D+ DA+LL GATWPRDLPIP R+ EGIHFAM FL++WQ+ Q G+ +D + A DKDVIVIGGGDTGCDCI TSLRQGAKSIT+FEILP PP R+ DNPWPQFPR+FKVDYGHEEV +K+G+ DPR+++T S EF+ D + GIR+V V W KD+ GRW + SEK+Y+C L LLAMGFLGPE Y+ L DPRSN T +G Y T VFAAGDCRRGQSLVVWAI+EGRQAAREVD FL+G + LPG GGVI
Sbjct: 9 PPKDGLYDPVNERDACGVGFIVAIDGRRSHKIVRDAEKLAMRMNHRGACACDNDTGDGAGVLTAIPHAFYAQELRDQQSVDLPPFGRYATGIVFLDKTHHQDSEAAFALLAEECQLQVLCWRTVPTDSAGIGDVARNSEPFMRQVFVTGDQ-----DDETLKRQVFVLRKRATHQIP--------RPGTRFYVCSLSLYVVVYKGQFTSDQLWTYFKDLKSPNYDTYLALVHTRFSTNTFPSWERAHPLRCVAHNGEINTLRGNVNLMKAREGVMKSSAFGSDLKKLYPVVEPNLSDSGAVDCVLEFLVMAGNRPMPEAVMTMVPEAWQNDRTMPADKRDFYHWSACAMEPWDGPALLTFTDGRYVGAILDRNGLRPSRFYVMKDNVMVMASEVGVYDTDPSNVLLKSRLKPGRMLLVDTKERTMIQDVEVKKRIAASRPHTLWLKEQILMADLRAEHEGIKKNAVVTNGNVEEVVDTVDTGSVYGITDEGLAKVWDGDRRLSLFSYTIETISFLLLPMMQTKKEALGSMGNDAPLACLSSFHPLPYDYFKQLFAQVTNPPIDPFREKVVMSLKCPIGPEANLLEPSEKQCHRLLLPHPILSLYDLEVIKATRHRGWKTKVIDITFDTEEGPKVLAKALDRICEEACKAAQDNYQFIVLSDRKAGASRIPVSVLLALGATHHYLIEERQRMKVGLILETAEAREVHHMCVLLGYGADAVCPYLVFEISQALRKEGVLDSSLTDDVLYRNYSDAMERGIAKVMAKMGISTLQSYKGAQIFEAVGLADEVIEKCFRGTHSRIGGVTFEILAKEAFERHYLSYGDHTCDMLVLRNPGLYHWRSGGEKHINDPLSIANLQEAAVNKSSNAYDRFRESTMECVKSCALRGQLELVTSDN---PIDVSLVEPASEIVKRFATGAMSFGSISLEAHTTLALAMNKVGGKSNTGEGGEDADRYLDQDPEHNKRSAIKQVASGRFGVTSSYLAHADDLQIKMAQGAKPGEGGELPGHKVTKEIATTRHSVPGVGLISPPPHHDIYSIEDLAELIYDLKCANPNARISVKLVSEVGVGVVASGVAKGKAEHIVISGHDGGTGASSWTGIKNAGLPWELGVAETHQVLVLNNLRSRVVLQADGQIRTGFDVLVAALLGADEFGFSTAPLIVMGCTMMRKCHLNTCPVGIATQDPVLRKKFEGKPEHVINYLFMLAEEIRGHMASLGVRKFQDLVGRTDLLRVGDTGNPKAKTLN---FSLILKNALHMRPGVNIV--------------------------------------------------GGSQRQDFQLEKRLDNELIEAAAPVLQGVKSRVDIVMKISNEDRAFASTLSYHISMRRGDAGLPDR-SINVRLTGSAGQSFCAFMTKGIHVTLEGDANDYVGKGLSGGEVIIYPPRDSSPDFKSELNVIVGNVCLYGATSGKAFFRGIAAERFSVRNSGAVAVVEGVGDHGCEYMTGGCAVILGLTGRNFAAGMSGGIAYILDVDDSFRSKCNMEMVELFPLELEEDLNYVKSLLKEFQEKTGSLIAEDLLRTWPAPASRFVKVFPCEYQRAL---RQMAEEGAQQAVQQ---AVNGKLN----------ESKVRDIEESVLDGVFEKKRLDKILDKTRGFIKYKRETGMYRLAEKRLKDWEEIYNFGHVRKGLRVQAARCMECGVPFCQS-SHGCPLGNIIPKWNDLVFHNSWKEALNQLLQTNNFPEFTGRVCPAPCEGACVLGISEPPVTIKNIECAIIDHAFEQGWINPELPVLRTEKKVAVVGSGPSGLAAAHQLNKAGHLVTVFERNDRVGGLLQYGIPTMKLSKEVVQRRVSLLAKEGIEFKTNINVGKDISAKDLYEEYDALLLCTGATWPRDLPIPGRQLEGIHFAMTFLESWQKKQMGNIVDQPYLLAKDKDVIVIGGGDTGCDCIATSLRQGAKSITTFEILPEPPGKRARDNPWPQFPRVFKVDYGHEEVQLKFGR-DPRQFSTLSKEFVDDGKGHVAGIRSVTVNWTKDETGRWKMEEAPDSEKVYKCDLVLLAMGFLGPEKYISSELDLTLDPRSNYHTPSGRYCTSVPKVFAAGDCRRGQSLVVWAISEGRQAAREVDAFLMGSSTLPGPGGVI 2063
BLAST of glutamate synthase vs. nr
Match: gi|1228004110|ref|XP_021933943.1| (glutamate synthase [NADH], amyloplastic isoform X2 [Zootermopsis nevadensis]) HSP 1 Score: 2538.45 bits (6578), Expect = 0.000e+0 Identity = 1278/2144 (59.61%), Postives = 1573/2144 (73.37%), Query Frame = 0
Query: 34 PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEV-GKLGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWN-QHVTLGDMR------KANKHEISTERRPIIKSLALLNDHSI----VARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNALN-LEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKC------------------TNPTARISVKLVSEVGVGVVASGVA----------KGKSEHIL---------ISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGDTLLPGLGGVI 2126
P K GLYDP E+DACGVGFIV IDG +SH ++RDAE+L+ RM HRGAC+ DND+GDGAGVL AIP F+ +E+ + LPP G+YATGI+FL + Q+S+ F LA L+VLCWR VP ++ +G +A+ SEP M QVFV + +D FVLRK A+H++ + RFYV SLS +VYKGQF QL+ YF DL P++ T++ +VHTRFSTNTFPSWERAHP R +AHNGEINTLRGN+NLMKAREGVM + FGS L +L+PVVE + SDSGA+D VLEFLVMAGNR +PEAVMT+VPEAWQND M K++FY +++C+ EPWDGPALLTF+DGRYVGAILDRNGLRPSR+YV DN+MVMASEVGVYD+DP +L K RL PGRMLLVDT E+ +IQD +KK IA SRPH W Q + + D+R K N + ++ ++ + + I +A++W GDRR+ +F YT+ETI L++PM TKKEALGSMGND PLACLS PL Y+YFKQLFAQVTNPPIDPFRE++VMSL+CPIGPE NLLEP+E+ C RL L +PILSL DLEV+K T GWKTKVIDIT++ L ALD+IC+EA KAA++ +QFI+LSDR G +RIP+S LA GA HH+LI R R K +ILET E REVH +CV++G+G DA+CPY+VFEI++ LR+E V+ S +T++V Y NY+ A++RGI+KVMAKMGIST+QSYK AQIFEAVGL++EV+++CF GT SRIGGVTF ILA+E+ RH L++G++ CD VLR+PG H+RSGGEKHINDP S+A LQEA ++ +Y ++ ++ +E ++ C +RGQLE+ ++ ID+ VEPASEIVKRFATGAMSFGSIS EAH+TLA+AMNK+GGKSNTGEGGE+ +RYL+QDP++N RSAIKQVASGRFGVT+SYLA+AD+LQIKMAQGAKPGEGGELPG KVT +IA TRHSVPGVGLISPPPHHDIYSIEDLA+LIYDLKC NP ARISVKLVSEVGVGVVASGVAKGK+EHI+ISGHDGGTGASSWTGIK AGLPWELG++ETHQVL +NNLRSR + Q ++ T P + ++ + + G+A +GK EH++ I GH G + + G +G + + T+N ++L+ MR G +++ GGS QDFQLE RLD E+I L G + I +I+NE RAF +TLS+ I+ R D+GLP+ I+++L GSAGQSFC F+ KGI VTLEGDANDYVGKGLSGGEVII+PP++S FKSE N+I GNVCLYGATSGKAF RG+AAERF VRNSGA+A+VEGVGDHGCEYMTGG +ILG TGRNFAAGMSGGIAY+LD + +F+ KCN EMV+L P+ +ED+ ++K L+EF +KTGS++A+++L+ W + FVKVFPREYQRAL + +E+ + ++++ +NG++ ES V DIE++V D K+ + ILDKTRGF+KYKRE MYR AE R KDW+EIY+F HVRKGL+ QAARCM+CGVPFC S SHGCPLGNIIPK+NDLVFH++WKEAL+QLLQTNNFPEFTGRVCPAPCEGACVLGI+EP VTIKNIECAIID+AFE+GWI P P R+ K VA+VGSGP+GLAAA QLNKAGHLVTV+ERN+R+GGLLQYGIPTMKLSK+VVQRRV L+A+EGI FK N++VGKDI+ D+ DA+LL GATWPRDLPIP R+ EGIHFAM FL++WQ+ Q G+ +D + A DKDVIVIGGGDTGCDCI TSLRQGAKSIT+FEILP PP R+ DNPWPQFPR+FKVDYGHEEV +K+G+ DPR+++T S EF+ D + GIR+V V W KD+ GRW + SEK+Y+C L LLAMGFLGPE Y+ L DPRSN T +G Y T VFAAGDCRRGQSLVVWAI+EGRQAAREVD FL+G + LPG GGVI
Sbjct: 9 PPKDGLYDPVNERDACGVGFIVAIDGRRSHKIVRDAEKLAMRMNHRGACACDNDTGDGAGVLTAIPHAFYAQELRDQQSVDLPPFGRYATGIVFLDKTHHQDSEAAFALLAEECQLQVLCWRTVPTDSAGIGDVARNSEPFMRQVFVTGDQ-----DDETLKRQVFVLRKRATHQIP--------RPGTRFYVCSLSLYVVVYKGQFTSDQLWTYFKDLKSPNYDTYLALVHTRFSTNTFPSWERAHPLRCVAHNGEINTLRGNVNLMKAREGVMKSSAFGSDLKKLYPVVEPNLSDSGAVDCVLEFLVMAGNRPMPEAVMTMVPEAWQNDRTMPADKRDFYHWSACAMEPWDGPALLTFTDGRYVGAILDRNGLRPSRFYVMKDNVMVMASEVGVYDTDPSNVLLKSRLKPGRMLLVDTKERTMIQDVEVKKRIAASRPHTLWLKEQGILMADLRAEHEGIKKNAVVTNGNVEEVVDTVDTGSVYGITDEGLAKVWDGDRRLSLFSYTIETISFLLLPMMQTKKEALGSMGNDAPLACLSSFHPLPYDYFKQLFAQVTNPPIDPFREKVVMSLKCPIGPEANLLEPSEKQCHRLLLPHPILSLYDLEVIKATRHRGWKTKVIDITFDTEEGPKVLAKALDRICEEACKAAQDNYQFIVLSDRKAGASRIPVSVLLALGATHHYLIEERQRMKVGLILETAEAREVHHMCVLLGYGADAVCPYLVFEISQALRKEGVLDSSLTDDVLYRNYSDAMERGIAKVMAKMGISTLQSYKGAQIFEAVGLADEVIEKCFRGTHSRIGGVTFEILAKEAFERHYLSYGDHTCDMLVLRNPGLYHWRSGGEKHINDPLSIANLQEAAVNKSSNAYDRFRESTMECVKSCALRGQLELVTSDN---PIDVSLVEPASEIVKRFATGAMSFGSISLEAHTTLALAMNKVGGKSNTGEGGEDADRYLDQDPEHNKRSAIKQVASGRFGVTSSYLAHADDLQIKMAQGAKPGEGGELPGHKVTKEIATTRHSVPGVGLISPPPHHDIYSIEDLAELIYDLKCANPNARISVKLVSEVGVGVVASGVAKGKAEHIVISGHDGGTGASSWTGIKNAGLPWELGVAETHQVLVLNNLRSRVVLQADGQIRTGFDVLVAALLGADEFGFSTAPLIVMGCTMMRKCHLNTCPVGIATQDPVLRKKFEGKPEHVINYLFMLAEEIRGHMASLGVRKFQDLVGRTDLLRVGDTGNPKAKTLN---FSLILKNALHMRPGVNIV--------------------------------------------------GGSQRQDFQLEKRLDNELIEAAAPVLQGVKSRVDIVMKISNEDRAFASTLSYHISMRRGDAGLPDR-SINVRLTGSAGQSFCAFMTKGIHVTLEGDANDYVGKGLSGGEVIIYPPRDSSPDFKSELNVIVGNVCLYGATSGKAFFRGIAAERFSVRNSGAVAVVEGVGDHGCEYMTGGCAVILGLTGRNFAAGMSGGIAYILDVDDSFRSKCNMEMVELFPLELEEDLNYVKSLLKEFQEKTGSLIAEDLLRTWPAPASRFVKVFPREYQRAL---RQLAEEGAQQAVQQ---AVNGKLN----------ESKVRDIEESVLDGVFEKKRLDKILDKTRGFIKYKRETGMYRLAEKRLKDWEEIYNFGHVRKGLRVQAARCMECGVPFCQS-SHGCPLGNIIPKWNDLVFHNSWKEALNQLLQTNNFPEFTGRVCPAPCEGACVLGISEPPVTIKNIECAIIDHAFEQGWINPELPVLRTEKKVAVVGSGPSGLAAAHQLNKAGHLVTVFERNDRVGGLLQYGIPTMKLSKEVVQRRVSLLAKEGIEFKTNINVGKDISAKDLYEEYDALLLCTGATWPRDLPIPGRQLEGIHFAMTFLESWQKKQMGNIVDQPYLLAKDKDVIVIGGGDTGCDCIATSLRQGAKSITTFEILPEPPGKRARDNPWPQFPRVFKVDYGHEEVQLKFGR-DPRQFSTLSKEFVDDGKGHVAGIRSVTVNWTKDETGRWKMEEAPDSEKVYKCDLVLLAMGFLGPEKYISSELDLTLDPRSNYHTPSGRYCTSVPKVFAAGDCRRGQSLVVWAISEGRQAAREVDAFLMGSSTLPGPGGVI 2064
BLAST of glutamate synthase vs. nr
Match: gi|1321333780|ref|XP_023319057.1| (putative glutamate synthase [NADPH] isoform X2 [Trichogramma pretiosum]) HSP 1 Score: 2536.53 bits (6573), Expect = 0.000e+0 Identity = 1303/2120 (61.46%), Postives = 1579/2120 (74.48%), Query Frame = 0
Query: 34 PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEP-DVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWNQHVTLGDMRKANK-HEISTERRPIIKSLALLNDHSI--------VARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNALN-LEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVG---VVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKV--DL-------LNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSI--EEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLS-KITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGDTLLPGLGGVI 2126
P+K GLYDPSLE +ACGVGFIV IDG +SH ++RDAE+LS RM HRGAC+ DND+GDGAGVL AIP +++ E+ + G LP G YATGILFL E++ FE +A DL+V+CWR VP +N LG +AKK EP M QVFV +P + LK FVLRK +SH + + R+YV SLS T+VYKGQ QL+ YF DL P F+T++ +VHTRFSTNTFPSWERAHP R+LAHNGEINTLRGN+NLMKAREGVM ++ FG +L +L+PVVE + SDSG+ D VLEFLVMAG RSLPEAVMT+VPEAWQND M K++FY +A+C EPWDGPALLTF+DGRYVGAILDRNGLRPSR+YVT DN+MVMASEVGVYD+ P ++ K RL PGRMLLVDT EK+II D +K +IA SRPH W VT+ +R+ +K E++ P + AL+ + I V R+W GD+R+ +FGYTLETI +L++PM TKKEALGSMGND PLACLS+ QPLIYEYFKQLFAQVTNPPIDPFRE+IVMSL CPIGPE N+LEP+E RL+LK+PILSL DLEV+K T GWKTK+ID TY + N L + LD++CKEA++AA +QFI+LSDR G R+P+S L+ GAVHHHLI R R K IILETGE REVH ICV++G+G DAICPY+VFE+A LR ++VI T+E + Y+ A++RGISKVMAKMGIST+QSYK AQIFEAVGL++EVV++CF GT SRIGGV F ILA E RH++A+ ++ D+ V+ +PG H+R+GGEKHINDP S+A LQE +N ++Y+ Y ++E ++ CT+RGQLE F ++K+ A+D++ VEPASEIVKRFATGAMSFGSIS EAH TLA+AMN++GGKSNTGEGGEN +RYLNQDP +N RSAIKQVASGRFGVT+SYLANAD+LQIKMAQGAKPGEGGELPG+KVT DIA TRHSVPGVGLISPPPHHDIYSIEDLA+LIYDLKC NP ARISVKLVSEVGVGVVASGVAKGK EH++ISGHDGGTGASSWTGIK AGLPWELG++ETHQ+LT+NNLRSR + Q ++ ++ E G+ ++A G + H+ + L + S H + L V RT + A LG +F LI G TD K+ DL L+ +L +A LRPGA+I GGS++QDFQLE R D E+I + + L+ + ++ IE INNE RAFG+TLS+ I+KRY + GLP+ ++I+LKGSAGQSFC F+ KG+ V LEGDANDYVGKGL GGE++I+PPKNS F S++N+I GNVCLYGATSG+AF RG+AAERF VRNSGA+ +VEGVGDHGCEYMTGG +ILG TGRNFAAGMSGGIAYVLD +G+F+ KCN EMV+LLP+ EDI F++E L+EFV KTGS++A ++L W F KVFP EYQRAL K E T + +I EP + I V N +S V DIED V D + KK LDK +GF+KYKR+ YR E R +DW+EIY+F+ VRKGLK QAARCM+CGVPFC S SHGCPLGNIIPK+NDLV+ DNWKEAL+QLLQTNNFPEFTGRVCPAPCEGACVLGI+EPAVTIKNIECAIID+AFE+GWI +PP R+GK +AI+GSGPAGLAAA QLNKAGHLVTVYERN+R+GGLLQYGIPTMKLSK+VVQRRV+L+A+EGI FK V+VG+DIT ++K ++DAVL+ GATWPRDLPIP R+ GIHFA+ FL+ WQ+ Q G+K L+ + A K+VI+IGGGDTGCDCI TSLR GAKSI +FEILP PP RS DNPWPQFPR+FK+DYGHEEV ++G+ DPR+++T S EFL D ++GIRTV V+W KD+AGRW V SEK+Y+C L LLAMGFLGPE YV + + D R N T G YAT GV+AAGDCRRGQSLVVWAITEGRQAAREVD L+ T LP +GGV+
Sbjct: 10 PKKQGLYDPSLEHEACGVGFIVAIDGKRSHKIVRDAEKLSARMHHRGACACDNDTGDGAGVLCAIPHQYYADELSQRGIDLPEFGCYATGILFLDKKTHAEAEAAFEKIAKECDLQVICWRDVPTDNSHLGQVAKKCEPYMRQVFVTGSQPTEELKR------QVFVLRKKSSHAIS--------KPGLRYYVCSLSLKTVVYKGQLTADQLWSYFTDLKSPKFETYLALVHTRFSTNTFPSWERAHPLRLLAHNGEINTLRGNVNLMKAREGVMRSELFGDKLKELYPVVEPNLSDSGSADCVLEFLVMAGQRSLPEAVMTMVPEAWQNDLTMAAEKRDFYHWAACVMEPWDGPALLTFTDGRYVGAILDRNGLRPSRFYVTKDNMMVMASEVGVYDTPPENVVLKSRLKPGRMLLVDTEEKQIIGDVELKLKIARSRPHSLWLKNQVTMDHLRQDHKSKELTNGVAPEVNGHALVKKNGITEQDGISAVNRVWSGDKRIALFGYTLETINLLLLPMIQTKKEALGSMGNDAPLACLSEFQPLIYEYFKQLFAQVTNPPIDPFREKIVMSLLCPIGPESNILEPDESYVHRLFLKHPILSLDDLEVIKHTQYDGWKTKIIDCTYSKTLGPNGLLETLDRVCKEADEAATGGYQFIVLSDRQAGPERVPVSILLSLGAVHHHLIECRSRMKVGIILETGEAREVHHICVLLGYGADAICPYLVFEMARNLRIDHVIDKSKTDEAIFMAYSEAMERGISKVMAKMGISTLQSYKGAQIFEAVGLADEVVEKCFRGTCSRIGGVNFDILAREGFARHRMAYWDDSFDTVVINNPGIYHWRAGGEKHINDPRSIAALQEYVNSKNWSAYENYRKVSMEVVQACTLRGQLE--FVKNKT-AVDLNLVEPASEIVKRFATGAMSFGSISLEAHQTLAIAMNRIGGKSNTGEGGENADRYLNQDPQFNRRSAIKQVASGRFGVTSSYLANADDLQIKMAQGAKPGEGGELPGYKVTSDIAATRHSVPGVGLISPPPHHDIYSIEDLAELIYDLKCANPNARISVKLVSEVGVGVVASGVAKGKGEHVVISGHDGGTGASSWTGIKSAGLPWELGVAETHQILTLNNLRSRMIVQADGQMRTGFDIVVAALLGADEFGLSTAPLIAMGCTMMRKCHL--------NTCPVGIATQDPHLRKKFAGSPEHVINFFFALAEEV--------RT-----IMASLGITKF----QDLI--GRTDLLKIREDLKSEKAKTLDLSNVLRSALDLRPGANIKGGSIQQDFQLEQRPDNELIKLVKPVLERETDSVSIEMTINNETRAFGSTLSYHISKRYGEEGLPDN-SVNIKLKGSAGQSFCAFMTKGVSVELEGDANDYVGKGLCGGEIVIYPPKNSE--FNSQDNVIVGNVCLYGATSGRAFFRGIAAERFSVRNSGAVVVVEGVGDHGCEYMTGGTALILGLTGRNFAAGMSGGIAYVLDVDGSFRSKCNPEMVELLPLNKKEDIDFVQELLEEFVRKTGSLIAKDLLDIWPEPIHRFFKVFPYEYQRALNQLKQEEEDTKTKTILPLEPEPDT-------IAVPNGNGQS-VIDIEDVVADPVVAQKK----LDKIKGFMKYKRQTGHYRPVEKRMEDWKEIYNFQGVRKGLKTQAARCMECGVPFCQS-SHGCPLGNIIPKWNDLVYQDNWKEALNQLLQTNNFPEFTGRVCPAPCEGACVLGISEPAVTIKNIECAIIDHAFEQGWIVANPPANRTGKKIAIIGSGPAGLAAAHQLNKAGHLVTVYERNDRVGGLLQYGIPTMKLSKEVVQRRVNLLAEEGITFKTGVNVGRDITTTELKESHDAVLICTGATWPRDLPIPGRDLNGIHFAVSFLEHWQKKQMGNKTPLNPALMAKGKNVIIIGGGDTGCDCIATSLRHGAKSIVTFEILPEPPKQRSDDNPWPQFPRIFKMDYGHEEVVHRFGE-DPRKFSTLSKEFLNDGKGNVSGIRTVNVQWTKDEAGRWKMDEVPNSEKVYKCDLVLLAMGFLGPEKYVAEELSTKMDGRGNFETPAGKYATSIPGVYAAGDCRRGQSLVVWAITEGRQAAREVDLALMKTTCLPVVGGVV 2068
BLAST of glutamate synthase vs. nr
Match: gi|936710657|ref|XP_014233296.1| (putative glutamate synthase [NADPH] isoform X1 [Trichogramma pretiosum] >gi|936710659|ref|XP_014233297.1| putative glutamate synthase [NADPH] isoform X1 [Trichogramma pretiosum] >gi|936710662|ref|XP_014233298.1| putative glutamate synthase [NADPH] isoform X1 [Trichogramma pretiosum] >gi|1321333777|ref|XP_023319056.1| putative glutamate synthase [NADPH] isoform X1 [Trichogramma pretiosum]) HSP 1 Score: 2535.37 bits (6570), Expect = 0.000e+0 Identity = 1305/2121 (61.53%), Postives = 1581/2121 (74.54%), Query Frame = 0
Query: 34 PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEVGKLGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEP-DVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWW-NQHVTLGDMRKANK-HEISTERRPIIKSLALLNDHSI--------VARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNALN-LEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKCTNPTARISVKLVSEVGVG---VVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKV--DL-------LNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSI--EEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLS-KITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGDTLLPGLGGVI 2126
P+K GLYDPSLE +ACGVGFIV IDG +SH ++RDAE+LS RM HRGAC+ DND+GDGAGVL AIP +++ E+ + G LP G YATGILFL E++ FE +A DL+V+CWR VP +N LG +AKK EP M QVFV +P + LK FVLRK +SH + + R+YV SLS T+VYKGQ QL+ YF DL P F+T++ +VHTRFSTNTFPSWERAHP R+LAHNGEINTLRGN+NLMKAREGVM ++ FG +L +L+PVVE + SDSG+ D VLEFLVMAG RSLPEAVMT+VPEAWQND M K++FY +A+C EPWDGPALLTF+DGRYVGAILDRNGLRPSR+YVT DN+MVMASEVGVYD+ P ++ K RL PGRMLLVDT EK+II D +K +IA SRPH W NQ VT+ +R+ +K E++ P + AL+ + I V R+W GD+R+ +FGYTLETI +L++PM TKKEALGSMGND PLACLS+ QPLIYEYFKQLFAQVTNPPIDPFRE+IVMSL CPIGPE N+LEP+E RL+LK+PILSL DLEV+K T GWKTK+ID TY + N L + LD++CKEA++AA +QFI+LSDR G R+P+S L+ GAVHHHLI R R K IILETGE REVH ICV++G+G DAICPY+VFE+A LR ++VI T+E + Y+ A++RGISKVMAKMGIST+QSYK AQIFEAVGL++EVV++CF GT SRIGGV F ILA E RH++A+ ++ D+ V+ +PG H+R+GGEKHINDP S+A LQE +N ++Y+ Y ++E ++ CT+RGQLE F ++K+ A+D++ VEPASEIVKRFATGAMSFGSIS EAH TLA+AMN++GGKSNTGEGGEN +RYLNQDP +N RSAIKQVASGRFGVT+SYLANAD+LQIKMAQGAKPGEGGELPG+KVT DIA TRHSVPGVGLISPPPHHDIYSIEDLA+LIYDLKC NP ARISVKLVSEVGVGVVASGVAKGK EH++ISGHDGGTGASSWTGIK AGLPWELG++ETHQ+LT+NNLRSR + Q ++ ++ E G+ ++A G + H+ + L + S H + L V RT + A LG +F LI G TD K+ DL L+ +L +A LRPGA+I GGS++QDFQLE R D E+I + + L+ + ++ IE INNE RAFG+TLS+ I+KRY + GLP+ ++I+LKGSAGQSFC F+ KG+ V LEGDANDYVGKGL GGE++I+PPKNS F S++N+I GNVCLYGATSG+AF RG+AAERF VRNSGA+ +VEGVGDHGCEYMTGG +ILG TGRNFAAGMSGGIAYVLD +G+F+ KCN EMV+LLP+ EDI F++E L+EFV KTGS++A ++L W F KVFP EYQRAL K E T + +I EP + I V N +S V DIED V D + KK LDK +GF+KYKR+ YR E R +DW+EIY+F+ VRKGLK QAARCM+CGVPFC S SHGCPLGNIIPK+NDLV+ DNWKEAL+QLLQTNNFPEFTGRVCPAPCEGACVLGI+EPAVTIKNIECAIID+AFE+GWI +PP R+GK +AI+GSGPAGLAAA QLNKAGHLVTVYERN+R+GGLLQYGIPTMKLSK+VVQRRV+L+A+EGI FK V+VG+DIT ++K ++DAVL+ GATWPRDLPIP R+ GIHFA+ FL+ WQ+ Q G+K L+ + A K+VI+IGGGDTGCDCI TSLR GAKSI +FEILP PP RS DNPWPQFPR+FK+DYGHEEV ++G+ DPR+++T S EFL D ++GIRTV V+W KD+AGRW V SEK+Y+C L LLAMGFLGPE YV + + D R N T G YAT GV+AAGDCRRGQSLVVWAITEGRQAAREVD L+ T LP +GGV+
Sbjct: 10 PKKQGLYDPSLEHEACGVGFIVAIDGKRSHKIVRDAEKLSARMHHRGACACDNDTGDGAGVLCAIPHQYYADELSQRGIDLPEFGCYATGILFLDKKTHAEAEAAFEKIAKECDLQVICWRDVPTDNSHLGQVAKKCEPYMRQVFVTGSQPTEELKR------QVFVLRKKSSHAIS--------KPGLRYYVCSLSLKTVVYKGQLTADQLWSYFTDLKSPKFETYLALVHTRFSTNTFPSWERAHPLRLLAHNGEINTLRGNVNLMKAREGVMRSELFGDKLKELYPVVEPNLSDSGSADCVLEFLVMAGQRSLPEAVMTMVPEAWQNDLTMAAEKRDFYHWAACVMEPWDGPALLTFTDGRYVGAILDRNGLRPSRFYVTKDNMMVMASEVGVYDTPPENVVLKSRLKPGRMLLVDTEEKQIIGDVELKLKIARSRPHSLWLKNQMVTMDHLRQDHKSKELTNGVAPEVNGHALVKKNGITEQDGISAVNRVWSGDKRIALFGYTLETINLLLLPMIQTKKEALGSMGNDAPLACLSEFQPLIYEYFKQLFAQVTNPPIDPFREKIVMSLLCPIGPESNILEPDESYVHRLFLKHPILSLDDLEVIKHTQYDGWKTKIIDCTYSKTLGPNGLLETLDRVCKEADEAATGGYQFIVLSDRQAGPERVPVSILLSLGAVHHHLIECRSRMKVGIILETGEAREVHHICVLLGYGADAICPYLVFEMARNLRIDHVIDKSKTDEAIFMAYSEAMERGISKVMAKMGISTLQSYKGAQIFEAVGLADEVVEKCFRGTCSRIGGVNFDILAREGFARHRMAYWDDSFDTVVINNPGIYHWRAGGEKHINDPRSIAALQEYVNSKNWSAYENYRKVSMEVVQACTLRGQLE--FVKNKT-AVDLNLVEPASEIVKRFATGAMSFGSISLEAHQTLAIAMNRIGGKSNTGEGGENADRYLNQDPQFNRRSAIKQVASGRFGVTSSYLANADDLQIKMAQGAKPGEGGELPGYKVTSDIAATRHSVPGVGLISPPPHHDIYSIEDLAELIYDLKCANPNARISVKLVSEVGVGVVASGVAKGKGEHVVISGHDGGTGASSWTGIKSAGLPWELGVAETHQILTLNNLRSRMIVQADGQMRTGFDIVVAALLGADEFGLSTAPLIAMGCTMMRKCHL--------NTCPVGIATQDPHLRKKFAGSPEHVINFFFALAEEV--------RT-----IMASLGITKF----QDLI--GRTDLLKIREDLKSEKAKTLDLSNVLRSALDLRPGANIKGGSIQQDFQLEQRPDNELIKLVKPVLERETDSVSIEMTINNETRAFGSTLSYHISKRYGEEGLPDN-SVNIKLKGSAGQSFCAFMTKGVSVELEGDANDYVGKGLCGGEIVIYPPKNSE--FNSQDNVIVGNVCLYGATSGRAFFRGIAAERFSVRNSGAVVVVEGVGDHGCEYMTGGTALILGLTGRNFAAGMSGGIAYVLDVDGSFRSKCNPEMVELLPLNKKEDIDFVQELLEEFVRKTGSLIAKDLLDIWPEPIHRFFKVFPYEYQRALNQLKQEEEDTKTKTILPLEPEPDT-------IAVPNGNGQS-VIDIEDVVADPVVAQKK----LDKIKGFMKYKRQTGHYRPVEKRMEDWKEIYNFQGVRKGLKTQAARCMECGVPFCQS-SHGCPLGNIIPKWNDLVYQDNWKEALNQLLQTNNFPEFTGRVCPAPCEGACVLGISEPAVTIKNIECAIIDHAFEQGWIVANPPANRTGKKIAIIGSGPAGLAAAHQLNKAGHLVTVYERNDRVGGLLQYGIPTMKLSKEVVQRRVNLLAEEGITFKTGVNVGRDITTTELKESHDAVLICTGATWPRDLPIPGRDLNGIHFAVSFLEHWQKKQMGNKTPLNPALMAKGKNVIIIGGGDTGCDCIATSLRHGAKSIVTFEILPEPPKQRSDDNPWPQFPRIFKMDYGHEEVVHRFGE-DPRKFSTLSKEFLNDGKGNVSGIRTVNVQWTKDEAGRWKMDEVPNSEKVYKCDLVLLAMGFLGPEKYVAEELSTKMDGRGNFETPAGKYATSIPGVYAAGDCRRGQSLVVWAITEGRQAAREVDLALMKTTCLPVVGGVV 2069
BLAST of glutamate synthase vs. nr
Match: gi|1228004100|ref|XP_021933936.1| (glutamate synthase [NADH], amyloplastic isoform X1 [Zootermopsis nevadensis] >gi|1228004102|ref|XP_021933939.1| glutamate synthase [NADH], amyloplastic isoform X1 [Zootermopsis nevadensis] >gi|1228004104|ref|XP_021933940.1| glutamate synthase [NADH], amyloplastic isoform X1 [Zootermopsis nevadensis] >gi|1228004106|ref|XP_021933941.1| glutamate synthase [NADH], amyloplastic isoform X1 [Zootermopsis nevadensis] >gi|1228004108|ref|XP_021933942.1| glutamate synthase [NADH], amyloplastic isoform X1 [Zootermopsis nevadensis]) HSP 1 Score: 2534.6 bits (6568), Expect = 0.000e+0 Identity = 1277/2144 (59.56%), Postives = 1572/2144 (73.32%), Query Frame = 0
Query: 34 PEKAGLYDPSLEKDACGVGFIVHIDGTQSHDLLRDAEQLSNRMEHRGACSADNDSGDGAGVLVAIPQEFFEKEV-GKLGHRLPPLGQYATGILFLGPGNLQESKDEFEALALTLDLKVLCWRRVPKENDCLGAIAKKSEPIMEQVFVVRKEPDVLKEDGMFVTNAFVLRKMASHRLDHHHGVWKGQNDKRFYVSSLSSTTLVYKGQFNPCQLFQYFLDLVDPDFKTHICIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNINLMKAREGVMANKQFGSQLSQLFPVVEGDTSDSGALDNVLEFLVMAGNRSLPEAVMTLVPEAWQNDPLMDETKKNFYRFASCSFEPWDGPALLTFSDGRYVGAILDRNGLRPSRYYVTTDNIMVMASEVGVYDSDPGKILQKGRLMPGRMLLVDTLEKKIIQDEVMKKEIALSRPHEAWWN-QHVTLGDMR------KANKHEISTERRPIIKSLALLNDHSI----VARIWQGDRRMPMFGYTLETIQMLMVPMFNTKKEALGSMGNDTPLACLSKLQPLIYEYFKQLFAQVTNPPIDPFRERIVMSLECPIGPEGNLLEPNERNCQRLWLKNPILSLTDLEVLKKTTVLGWKTKVIDITYEVSNALN-LEDALDKICKEAEKAARE-HQFIILSDRNIGETRIPISAGLASGAVHHHLINTRLRSKCAIILETGEIREVHQICVVVGFGVDAICPYMVFEIAEMLREENVISSDITNEVAYANYAAAVDRGISKVMAKMGISTMQSYKAAQIFEAVGLSNEVVDRCFNGTASRIGGVTFAILAEESLIRHKLAFGENECDSRVLRDPGFLHYRSGGEKHINDPTSVALLQEATKYENKASYQKYVDAALESIRDCTIRGQLEINFPEDKSKAIDIDEVEPASEIVKRFATGAMSFGSISYEAHSTLAVAMNKLGGKSNTGEGGENPERYLNQDPDYNMRSAIKQVASGRFGVTASYLANADELQIKMAQGAKPGEGGELPGFKVTIDIAKTRHSVPGVGLISPPPHHDIYSIEDLAQLIYDLKCTNPTARISVKLVSEVGVGVVASGVAKGKSEHILISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRDLAQLIYDLKC------------------TNPTARISVKLVSEVGVGVVASGVA----------KGKSEHIL---------ISGHDGGTGASSWTGIKGAGLPWELGLSETHQVLTMNNLRSRVVLQADGQMRTGFDVLVAALLGADEFGFSTAPLIAMGSTDSPKVDLLNFEKILTNASLLRPGASILGGSVEQDFQLEARLDAEIIPICQDALDGKPGTIVIEREINNEQRAFGATLSHEIAKRYSDSGLPEGCQIDIQLKGSAGQSFCVFLMKGIKVTLEGDANDYVGKGLSGGEVIIFPPKNSHQAFKSEENIIAGNVCLYGATSGKAFIRGVAAERFCVRNSGALAIVEGVGDHGCEYMTGGRVIILGCTGRNFAAGMSGGIAYVLDHEGNFKDKCNKEMVDLLPIIDDEDIIFLKENLQEFVDKTGSIVADNILKNWSSAQQMFVKVFPREYQRALLDQKAASEQTSSNSIEEPPKEINGRVTPEIRVTNDKEESPVADIEDAVKDKDMVAKKQEVILDKTRGFVKYKREPKMYRDAEDRQKDWQEIYDFKHVRKGLKKQAARCMDCGVPFCHSTSHGCPLGNIIPKFNDLVFHDNWKEALDQLLQTNNFPEFTGRVCPAPCEGACVLGINEPAVTIKNIECAIIDNAFEEGWIKPSPPKKRSGKTVAIVGSGPAGLAAADQLNKAGHLVTVYERNNRIGGLLQYGIPTMKLSKDVVQRRVDLMAQEGIIFKANVDVGKDITVDDIKNNNDAVLLTVGATWPRDLPIPNREAEGIHFAMEFLQTWQQNQHGDKMDLSKITAVDKDVIVIGGGDTGCDCIGTSLRQGAKSITSFEILPTPPDTRSTDNPWPQFPRLFKVDYGHEEVSVKWGKGDPRRYNTESIEFLKDEANKLTGIRTVQVEWVKDDAGRWVKQNVEGSEKIYECQLALLAMGFLGPENYVIQSFGLEQDPRSNIITKNGHYATPKAGVFAAGDCRRGQSLVVWAITEGRQAAREVDEFLVGDTLLPGLGGVI 2126
P K GLYDP E+DACGVGFIV IDG +SH ++RDAE+L+ RM HRGAC+ DND+GDGAGVL AIP F+ +E+ + LPP G+YATGI+FL + Q+S+ F LA L+VLCWR VP ++ +G +A+ SEP M QVFV + +D FVLRK A+H++ + RFYV SLS +VYKGQF QL+ YF DL P++ T++ +VHTRFSTNTFPSWERAHP R +AHNGEINTLRGN+NLMKAREGVM + FGS L +L+PVVE + SDSGA+D VLEFLVMAGNR +PEAVMT+VPEAWQND M K++FY +++C+ EPWDGPALLTF+DGRYVGAILDRNGLRPSR+YV DN+MVMASEVGVYD+DP +L K RL PGRMLLVDT E+ +IQD +KK IA SRPH W Q + + D+R K N + ++ ++ + + I +A++W GDRR+ +F YT+ETI L++PM TKKEALGSMGND PLACLS PL Y+YFKQLFAQVTNPPIDPFRE++VMSL+CPIGPE NLLEP+E+ C RL L +PILSL DLEV+K T GWKTKVIDIT++ L ALD+IC+EA KAA++ +QFI+LSDR G +RIP+S LA GA HH+LI R R K +ILET E REVH +CV++G+G DA+CPY+VFEI++ LR+E V+ S +T++V Y NY+ A++RGI+KVMAKMGIST+QSYK AQIFEAVGL++EV+++CF GT SRIGGVTF ILA+E+ RH L++G++ CD VLR+PG H+RSGGEKHINDP S+A LQEA ++ +Y ++ ++ +E ++ C +RGQLE+ ++ ID+ VEPASEIVKRFATGAMSFGSIS EAH+TLA+AMNK+GGKSNTGEGGE+ +RYL+QDP++N RSAIKQVASGRFGVT+SYLA+AD+LQIKMAQGAKPGEGGELPG KVT +IA TRHSVPGVGLISPPPHHDIYSIEDLA+LIYDLKC NP ARISVKLVSEVGVGVVASGVAKGK+EHI+ISGHDGGTGASSWTGIK AGLPWELG++ETHQVL +NNLRSR + Q ++ T P + ++ + + G+A +GK EH++ I GH G + + G +G + + T+N ++L+ MR G +++ GGS QDFQLE RLD E+I L G + I +I+NE RAF +TLS+ I+ R D+GLP+ I+++L GSAGQSFC F+ KGI VTLEGDANDYVGKGLSGGEVII+PP++S FKSE N+I GNVCLYGATSGKAF RG+AAERF VRNSGA+A+VEGVGDHGCEYMTGG +ILG TGRNFAAGMSGGIAY+LD + +F+ KCN EMV+L P+ +ED+ ++K L+EF +KTGS++A+++L+ W + FVKVFP EYQRAL + +E+ + ++++ +NG++ ES V DIE++V D K+ + ILDKTRGF+KYKRE MYR AE R KDW+EIY+F HVRKGL+ QAARCM+CGVPFC S SHGCPLGNIIPK+NDLVFH++WKEAL+QLLQTNNFPEFTGRVCPAPCEGACVLGI+EP VTIKNIECAIID+AFE+GWI P P R+ K VA+VGSGP+GLAAA QLNKAGHLVTV+ERN+R+GGLLQYGIPTMKLSK+VVQRRV L+A+EGI FK N++VGKDI+ D+ DA+LL GATWPRDLPIP R+ EGIHFAM FL++WQ+ Q G+ +D + A DKDVIVIGGGDTGCDCI TSLRQGAKSIT+FEILP PP R+ DNPWPQFPR+FKVDYGHEEV +K+G+ DPR+++T S EF+ D + GIR+V V W KD+ GRW + SEK+Y+C L LLAMGFLGPE Y+ L DPRSN T +G Y T VFAAGDCRRGQSLVVWAI+EGRQAAREVD FL+G + LPG GGVI
Sbjct: 9 PPKDGLYDPVNERDACGVGFIVAIDGRRSHKIVRDAEKLAMRMNHRGACACDNDTGDGAGVLTAIPHAFYAQELRDQQSVDLPPFGRYATGIVFLDKTHHQDSEAAFALLAEECQLQVLCWRTVPTDSAGIGDVARNSEPFMRQVFVTGDQ-----DDETLKRQVFVLRKRATHQIP--------RPGTRFYVCSLSLYVVVYKGQFTSDQLWTYFKDLKSPNYDTYLALVHTRFSTNTFPSWERAHPLRCVAHNGEINTLRGNVNLMKAREGVMKSSAFGSDLKKLYPVVEPNLSDSGAVDCVLEFLVMAGNRPMPEAVMTMVPEAWQNDRTMPADKRDFYHWSACAMEPWDGPALLTFTDGRYVGAILDRNGLRPSRFYVMKDNVMVMASEVGVYDTDPSNVLLKSRLKPGRMLLVDTKERTMIQDVEVKKRIAASRPHTLWLKEQGILMADLRAEHEGIKKNAVVTNGNVEEVVDTVDTGSVYGITDEGLAKVWDGDRRLSLFSYTIETISFLLLPMMQTKKEALGSMGNDAPLACLSSFHPLPYDYFKQLFAQVTNPPIDPFREKVVMSLKCPIGPEANLLEPSEKQCHRLLLPHPILSLYDLEVIKATRHRGWKTKVIDITFDTEEGPKVLAKALDRICEEACKAAQDNYQFIVLSDRKAGASRIPVSVLLALGATHHYLIEERQRMKVGLILETAEAREVHHMCVLLGYGADAVCPYLVFEISQALRKEGVLDSSLTDDVLYRNYSDAMERGIAKVMAKMGISTLQSYKGAQIFEAVGLADEVIEKCFRGTHSRIGGVTFEILAKEAFERHYLSYGDHTCDMLVLRNPGLYHWRSGGEKHINDPLSIANLQEAAVNKSSNAYDRFRESTMECVKSCALRGQLELVTSDN---PIDVSLVEPASEIVKRFATGAMSFGSISLEAHTTLALAMNKVGGKSNTGEGGEDADRYLDQDPEHNKRSAIKQVASGRFGVTSSYLAHADDLQIKMAQGAKPGEGGELPGHKVTKEIATTRHSVPGVGLISPPPHHDIYSIEDLAELIYDLKCANPNARISVKLVSEVGVGVVASGVAKGKAEHIVISGHDGGTGASSWTGIKNAGLPWELGVAETHQVLVLNNLRSRVVLQADGQIRTGFDVLVAALLGADEFGFSTAPLIVMGCTMMRKCHLNTCPVGIATQDPVLRKKFEGKPEHVINYLFMLAEEIRGHMASLGVRKFQDLVGRTDLLRVGDTGNPKAKTLN---FSLILKNALHMRPGVNIV--------------------------------------------------GGSQRQDFQLEKRLDNELIEAAAPVLQGVKSRVDIVMKISNEDRAFASTLSYHISMRRGDAGLPDR-SINVRLTGSAGQSFCAFMTKGIHVTLEGDANDYVGKGLSGGEVIIYPPRDSSPDFKSELNVIVGNVCLYGATSGKAFFRGIAAERFSVRNSGAVAVVEGVGDHGCEYMTGGCAVILGLTGRNFAAGMSGGIAYILDVDDSFRSKCNMEMVELFPLELEEDLNYVKSLLKEFQEKTGSLIAEDLLRTWPAPASRFVKVFPCEYQRAL---RQMAEEGAQQAVQQ---AVNGKLN----------ESKVRDIEESVLDGVFEKKRLDKILDKTRGFIKYKRETGMYRLAEKRLKDWEEIYNFGHVRKGLRVQAARCMECGVPFCQS-SHGCPLGNIIPKWNDLVFHNSWKEALNQLLQTNNFPEFTGRVCPAPCEGACVLGISEPPVTIKNIECAIIDHAFEQGWINPELPVLRTEKKVAVVGSGPSGLAAAHQLNKAGHLVTVFERNDRVGGLLQYGIPTMKLSKEVVQRRVSLLAKEGIEFKTNINVGKDISAKDLYEEYDALLLCTGATWPRDLPIPGRQLEGIHFAMTFLESWQKKQMGNIVDQPYLLAKDKDVIVIGGGDTGCDCIATSLRQGAKSITTFEILPEPPGKRARDNPWPQFPRVFKVDYGHEEVQLKFGR-DPRQFSTLSKEFVDDGKGHVAGIRSVTVNWTKDETGRWKMEEAPDSEKVYKCDLVLLAMGFLGPEKYISSELDLTLDPRSNYHTPSGRYCTSVPKVFAAGDCRRGQSLVVWAISEGRQAAREVDAFLMGSSTLPGPGGVI 2064 The following BLAST results are available for this feature:
BLAST of glutamate synthase vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 2
BLAST of glutamate synthase vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of glutamate synthase vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold455_size166772:130988..144770- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold455_size166772-processed-gene-0.25 ID=snap_masked-scaffold455_size166772-processed-gene-0.25|Name=glutamate synthase|organism=Tigriopus kingsejongensis|type=gene|length=13783bp|location=Sequence derived from alignment at scaffold455_size166772:130988..144770- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'glutamate synthase' has the following synonyms
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