BLAST of duplex-specific nuclease vs. L. salmonis genes
Match: EMLSAG00000012345 (supercontig:LSalAtl2s:LSalAtl2s900:155793:157148:-1 gene:EMLSAG00000012345 transcript:EMLSAT00000012345 description:"maker-LSalAtl2s900-snap-gene-0.28")

HSP 1 Score: 245.743 bits (626), Expect = 1.862e-77
Identity = 141/380 (37.11%), Postives = 205/380 (53.95%), Query Frame = 0

Query:  127 SCPGAYVQL--VRERHVSVMC-------------------------LGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGP-EGRGVRVVIGWLAPSGSLKPQIQVCHDPVSEATFYARHTLFGG--AIGAREVASARPSYFKEGG-FYKQTSANQAYKLRTQRETLKSILGPVVGARIFNYKTS-YLAKGHLAPDADF--IYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKW--FFLYQK-NIAKGYTYCCRYQDFQAVIDWVPDVGNPEVLEN 469
            SCPG  ++    RE  VS  C                          GE   +++S     W  LTC+R IRE   +    PCGP     V + IGW + + +   Q+ VCH+   E T+++ HT+ G   + G  E   +RPSYFKEG  F+ ++SA+ +YKLRTQ++    I G       F+ + S YLA+GHL PD DF  I+     ATY+++NVAP WQS NNGNWK +E ++R Y SL   +  V+TGT   LKL +  G+   ++L            +LIPVP+  WK++++P   +AIVF+G N+PF E    +   +CPD+C    W  F  +++  ++KGY YCC Y   + VI W+PD+GNP +L+N
Sbjct:   13 SCPGGALRFNKQRETRVSTKCNPIADNNNNKNSTTTLVIHDYIDPESGEEKRIELSPNDTSWKTLTCTRKIREST-KTRLQPCGPISYSNVMIDIGWTSDT-NFTTQLSVCHNIADETTWFSNHTIQGSLSSFGTFEKDDSRPSYFKEGSQFFTKSSASTSYKLRTQKDKFIKIFG--AQNDFFSIRRSFYLARGHLTPDGDFDLIHNSLADATYHYINVAPMWQSINNGNWKVLEGSIRLYVSLLSEDFVVYTGTYGVLKLPNKNGVKIPIYLGED---------ELIPVPKLIWKILFNPRREEAIVFIGSNNPFLE----QIETVCPDLCEDTHWNDFLEHRRFEVSKGYLYCCSYHSVKKVISWLPDLGNPFILQN 375          

BLAST of duplex-specific nuclease vs. L. salmonis genes
Match: EMLSAG00000000465 (supercontig:LSalAtl2s:LSalAtl2s106:710470:724606:-1 gene:EMLSAG00000000465 transcript:EMLSAT00000000465 description:"maker-LSalAtl2s106-augustus-gene-7.8")

HSP 1 Score: 184.111 bits (466), Expect = 1.850e-50
Identity = 132/420 (31.43%), Postives = 198/420 (47.14%), Query Frame = 0

Query:   70 PLPPRPDR-GCVVDLHDHSVTNLPLFYHPLSEDFLLPQAGEKPGQRMLALPWSEPFTVSC---PGAYVQLVRER-----HVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEG---------RGVRVVIGWLAPSGSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYF-KEGG---FYKQTSAN---QAYKLRTQRETLKSILG--PVVGARIFNYK---TSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVP 459
            P PP P R GC + L      N PL     S  FL P    K   R+  +P      ++C    G ++    ER      V + C  +   L   G  V   +  CS   +  +     + C PEG         R   + +GW   SG ++ Q+++C D +   T + +H + G +I   +  S RP++   E G    + + S N   QAY  ++Q++T+K I G   + G  +       T ++AKGHL+PDA F+Y   Q  TY+F+N APQ+QSFNNGNW+A+E AVR+ A    + L V+TGT + L+        K + L  K          LIP P +FWK+V+ P   +A+ FVG N+ F + +P     IC D+C    W    + ++ KG+ YCC   D    I + P
Sbjct:  434 PTPPPPTRNGCKILLDKLPEKNTPLITSNGS--FLYPTYI-KDEARITLVPQGSTVELNCHRSRGNFLLYKEERISKIKSVKLTCTNDK--LYTEGMEVNPADYKCSSKNQPSLIITRNSKCSPEGIDKRKTDLGRITHISLGWNFRSGFIE-QVELCIDELFYGTLWTKHNVVGKSIEFSDKDSDRPAFIVDETGQKRLFGKRSTNKITQAYAKKSQKKTIKEITGHTTIYGLPMIETNRKGTLFMAKGHLSPDAAFVYDGEQEGTYFFVNAAPQYQSFNNGNWRALELAVRDLAEKLHSTLTVYTGTYEILEFYE-----KQIFLLEK---------KLIPAPRYFWKVVHDPSTKKAVAFVGYNNVFSKTSPK---PICKDVCDQIPWVDWERDSLYKGFMYCCEVDDLNKAISYSP 830          

HSP 2 Score: 182.956 bits (463), Expect = 4.840e-50
Identity = 132/419 (31.50%), Postives = 198/419 (47.26%), Query Frame = 0

Query:   70 PLPPRPDR-GCVVDLHDHSVTNLPLFYHPLSEDFLLPQAGEKPGQRMLALPWSEPFTVSC---PGAYVQLVRER-----HVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEG---------RGVRVVIGWLAPSGSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYF-KEGG---FYKQTSANQ---AYKLRTQRETL--KSILGPVVGARIFNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDV 461
            P PP P R GC + L      N PL     S  FL P    K   R+  +P      ++C    G ++    ER      V + C  +   L   G  V   +  CS   +  +     + C PEG         R   + +GW   SG ++ Q+++C D +   T + +H + G  I  R+  S RP++   E G    + + S NQ   AY   +Q  ++  +SI+ P   ++       YLAKGHL+PD+ F+Y   Q  TY+F+NVAPQ+QSFN GNW A+E AVR+ A    + L V+TGT + L+L       K + L  K           IPVP +FWK+++ P   +A+ FVG N+   + +P     IC D+C    W    ++++ KGY YCC  +D    I + PD+
Sbjct: 1080 PTPPPPTRNGCKILLDKLPEKNTPLITSNGS--FLYPTYI-KDDARITLVPQGSTVELNCHRSRGNFLLYKEERVSKIESVKLTCTNDK--LYTEGMEVNPADYKCSSKNQPSLIITRNSKCSPEGIDKRKTDLERITHISLGWNFRSGYIE-QVEICIDELFYGTLWTKHYVEGQNIEMRDKYSGRPAFIVDETGKKRLFGKRSTNQITKAYAKNSQNTSIYDQSIMNPSKSSKF------YLAKGHLSPDSAFVYDGEQEGTYFFVNVAPQYQSFNKGNWLALEYAVRDLAKNQYSKLTVYTGTYEILELHQ-----KQIFLLEK---------KFIPVPRYFWKVLHDPARKKAVAFVGYNNVLRKTSPK---PICTDVCDQIPWVDWERESLFKGYMYCCNVEDLNKAISYSPDL 1469          

HSP 3 Score: 177.178 bits (448), Expect = 3.352e-48
Identity = 124/432 (28.70%), Postives = 194/432 (44.91%), Query Frame = 0

Query:   79 CVVDLHDHSVTNLPLFYHPLSEDFLLPQAGEKPGQRMLALPWSEPFTVSCPGAYVQLVRERH---------VSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEG---------RGVRVVIGWLAPSGSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFKEGG-------FYKQTSANQAYKLRTQRETLKSILG----PVVGARI-----FNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDVGN----PEVLENILP 472
            C ++L   +  + P      +   + P+       R++ +   E   + C G+  +L +  H         +S+ C  + G    S ++V    L+C+  +  ++ER     C P G           V + +G+   S S  P + +CHD     T +  HT+ G +I  R+    RP++    G       F   T  N  Y   TQ +T+K +LG     V G R+         T Y AKGHL+PDA FIY   Q  TY++ NVAPQ+QSFNN NWK++E+  R +AS    NL+V+TGT   LKL +    P  + +              +P P ++WK++Y P  N+AI F+GLN+P+          IC +ICA   +  +       GYT C      +  I  +PD+      PE++  + P
Sbjct:   25 CKINLDSRTGNHQPFILKEKTNQVIYPKES-----RIITIGDGENIVIDCHGS--KLSKATHYGIPSGLSKISLSC--DNGAFRNSPKIVKVEILSCTSKVYPQLERKS-VKCSPVGADDRLTDLDDLVLINVGF-NFSSSYSPLMNICHDEKVYGTIWTHHTIRGESINNRDRTIDRPTFRTNIGRSKIYYPFTTMTQMNSQYSKSTQVKTIKKLLGIYTIMVDGKRVPIIDESRSGTHYFAKGHLSPDAAFIYSAEQDGTYFYSNVAPQFQSFNNRNWKSIESTARKWASDNKQNLEVYTGTASILKLPNKQSQPTEIKMF--------PSLKYVPAPMYYWKVLYDPEANEAIAFIGLNNPY---ERKAHNHICTNICAQTVFDDVDFYKFEAGYTMCXEVSQLRMSISSIPDLSKEGKWPELMGKLGP 434          

HSP 4 Score: 144.436 bits (363), Expect = 4.435e-37
Identity = 85/239 (35.56%), Postives = 122/239 (51.05%), Query Frame = 0

Query:  247 FYKQTSANQAYKLRTQRETLKSILGP----VVGARI-----FNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDVGN----PEVLENILP 472
            F   T  N  Y   TQ +T++++ G     V G  I         T+Y AKGHL+PDA FIY   Q  TY++ NVAPQ+QSFNN NWK++E+  R +AS    NL+V+TGT   L L ++   P  + L         S    +P P ++WK++Y P  N+AI F+GLN+P+          IC +ICA   +  +       GYT CC     +  I  +PD+      PE++  + P
Sbjct:  853 FTTMTQMNSQYSKSTQVKTIETLFGNNSIIVDGKEIPIIDESRSGTNYFAKGHLSPDAAFIYSVEQDGTYFYSNVAPQFQSFNNRNWKSIESTARKWASDNKRNLEVYTGTASILNLLNEQCKPINIELF--------SDRQYVPAPMYYWKVLYDPEANEAIAFIGLNNPY---ERKAHNHICSNICAQTVFDDVDFYKFEAGYTMCCEVSQLRMSISSIPDLSKEGKWPELMGKLGP 1080          

BLAST of duplex-specific nuclease vs. nr
Match: gi|1276314427|gb|ATU82404.1| (venom nuclease 1 [Lethocerus distinctifemur])

HSP 1 Score: 231.491 bits (589), Expect = 1.219e-67
Identity = 143/406 (35.22%), Postives = 210/406 (51.72%), Query Frame = 0

Query:   71 LPPRPDRGCVVDLHDH-SVTNLPLFY-HPLSEDFLL--PQAGEKPGQRMLALPWSEPFTVSCPGAYVQL--VRERHVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEGRGVRVVIGWLAPSGSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFK-EGGFYKQTSANQAYKLRTQRETLKSILGPVVGARIFNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDVGNPEVLEN 469
            LP R  +GC + L++H    N PLF     ++D  L  P+   K G   ++L   E   VSCPG    L   +E + S  C  +   L + G++     L CS      +   DR PC     GV V +G+   +      ++VCH+  +  ++YA HT++G ++  + + S RP + + E  FYK  + + A+    Q E  +SI G    A  F  K  +LA+GHLAPDADF+Y  WQ  TY+++N APQWQS N G+W ++E  VR  AS  G +LDV TGT   L+L+ D    K ++L   +          +PVP+  WK++++P  N  I  V  N+PF    P     +CPDIC +  W  L Q + +KGY YCC+YQDF   +D+ P      VL+N
Sbjct:   21 LPARKSQGCSISLNEHLPKKNEPLFLIRSANKDLKLAMPETSGKLGS--ISLRPGEEVIVSCPGKQNSLGITQEEYSSAFC-HQDQQLTIKGRLFNSNMLECSHRSAAALLVTDR-PCA-NNNGVMVELGFKVEN--WHSLVEVCHNIKNSLSYYASHTVYGSSLAGKVIQSNRPGFSRGEKIFYKGFNPDAAFSQNNQVEVFESIFGKKKTAEYFESK-KFLARGHLAPDADFLYGSWQHLTYFYINTAPQWQSINAGHWLSIENLVRKIASNRGVDLDVATGTYGVLRLRDDKNREKEVYLQEDNR---------LPVPDVMWKVLHNPKDNSCIAIVASNNPFLSSPPK---TLCPDICRANNWPDL-QSDFSKGYVYCCKYQDFVRAVDYAPRFMCKSVLKN 405          

BLAST of duplex-specific nuclease vs. nr
Match: gi|1325324194|ref|XP_023343940.1| (uncharacterized protein LOC111713331 [Eurytemora affinis] >gi|1325324196|ref|XP_023343941.1| uncharacterized protein LOC111713331 [Eurytemora affinis] >gi|1325324198|ref|XP_023343942.1| uncharacterized protein LOC111713331 [Eurytemora affinis])

HSP 1 Score: 231.876 bits (590), Expect = 1.309e-67
Identity = 153/452 (33.85%), Postives = 223/452 (49.34%), Query Frame = 0

Query:   10 MLVVHMLLPRFLVLEIVLFFHFLHNGHACRKQIQRGLDHIRAPIAVNETVSMAIVTTATPPLPPRPDRGCVVDLHDHSVTNLPLFYHPLSEDFLLPQAGEKPGQRMLALPWSEPFTVSCPGAYVQLVRERHVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEGRGVRVVIGWLAPSGSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFKEGG-FYKQTSANQAYKLRTQRETLKSILGPVVGARIFNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKW--FFLYQKNIAKGYTYCCRYQDFQAVIDWV 458
            M V   LLPRFLVLE+VL  HF     AC   + RG                          P      C++ L      + PLFY   + D++ P AG     R+L +   E   +SCPG+ +       + V+C  +G  L V    V  + L C +  R   ER    PCGP   G  V +G+     S KP +  CH+  SE T+Y +HTL G  +    +   RP+ FKEG  FYK+ SA+  Y  R Q + L SILG      + N  +S+LA+GHLAPDADF+Y++WQ ATYY+ N+APQWQS NNGNW+ +E ++R        +L++F+GTL  L++  + G+ + + L    +         IPVPE+F+K++     +++I     N+PF E    R   +C DIC    W   F  +  +  G  +CC   +  +++ WV
Sbjct:    1 MFVDKFLLPRFLVLELVLASHFFQFTKACL--LNRGSG------------------------PGDGTESCLIPLDSSDWKHKPLFYTQ-NRDWIYPDAG-----RILRVVSGETILLSCPGSSLIYSSSPSLLVLC--KGTELQVKNTKVRESQLGCVKPPRNE-ERRTGEPCGPLQVGEIVELGY-KEDFSFKPVLSFCHEVDSEQTYYTQHTLRGSTLQFNSIDQTRPA-FKEGKLFYKKISASATYSQRNQEKVLTSILGQDRSRELLN--SSFLARGHLAPDADFLYQDWQDATYYYGNIAPQWQSINNGNWRRLENSIRTKGKKLYRDLEIFSGTLGILQVNGETGL-QEIWLGTDGSK------KYIPVPEYFFKIIVDSVESKSIGIFCTNNPFLE-RVLRSTVLCTDICDETGWGDKFKNRFEVGDGLIFCCSPAEMYSLVPWV 405          

BLAST of duplex-specific nuclease vs. nr
Match: gi|217272469|dbj|BAH02823.1| (duplex-specific nuclease [Paralithodes camtschaticus])

HSP 1 Score: 228.794 bits (582), Expect = 1.526e-66
Identity = 126/361 (34.90%), Postives = 193/361 (53.46%), Query Frame = 0

Query:  112 GQRMLALPWSEPFTVSCPGAYVQLVRERHVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEGRGVRVVIGWLAPSGSLKPQ-IQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFKEGGFYKQTSANQAYKLRTQRETLKSILGP--VVGARIFNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDVGNPEVLEN 469
            G+R++++P     T++C G+ +  +    V   C G G +L + G      +L CS  ++E + R+    CG   +G R  IG+    GS+  + I VC +PVSE T Y  H L G  I A+++ ++RPS+    GF+   S +  Y   +Q + +  ILG   +    I   +  Y AKGH++PDADF+    Q ATYYF+N  PQWQ+FNNGNWK +E A R+ A   G++L V++G    L+L    G P  + L   +      G +++PVP   WK+VY    ++A   VG+N+P     P     +C D+C+S  W      ++A GYTYCC   D +  I ++PD+GN  +L N
Sbjct:   59 GKRIVSVPSGSSLTLACSGSELINLGMEAVEAKCAG-GVMLAIEGTEWEIWSLGCSNHVKETIRRN-LGTCGEADQGDRHSIGFEYYGGSIYYELISVCFEPVSETTLYTEHVLHGANIAAKDIETSRPSFKTSTGFFS-VSMSTVYSQASQLQLMTDILGDSDLANNIIDPSQQLYFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDINGNPVDILLGLSE------GKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHITTAPS---PLCSDLCSSLTWIDFNLDDLAHGYTYCCAVDDLRQAIPYIPDLGNVGLLTN 407          

BLAST of duplex-specific nuclease vs. nr
Match: gi|290784917|dbj|BAI79321.2| (duplex-specific nuclease [Chionoecetes opilio])

HSP 1 Score: 224.942 bits (572), Expect = 4.001e-65
Identity = 130/382 (34.03%), Postives = 201/382 (52.62%), Query Frame = 0

Query:   95 YHPL----SEDFLLPQAGEKPGQRMLALPWSEPFTVSCPGAYVQLVRERHVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEGRGVRVVIGW-LAPSGSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFKEGGFYKQTSANQAYKLRTQRETLKSILGP--VVGARIFNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDVGNPEVLEN 469
            Y PL    S DF+LP   E+ G +++ +      T++CPG  +  + +      CL + GLL +        +L C+R ++E + RD    CG E  G    IG+ +   G  K  I+VC +P SE T +  H + G  I A+++ ++RPS+    GF+   S  +AY   +Q   + ++LG   +  + I  +K  Y AKGH++PDADF+    Q A+YY++N  PQWQ FNNGNW+ +E A R+ A   G +L V +G    L+L    G P  + L     DD +    ++P P   WK+VY    N A   VG+N+PF    P    ++C D+C+S  W      ++A GYTYCC  +D +A +  VPD+G+  +L +
Sbjct:   37 YSPLILDSSFDFVLPV--EEDGNKIVRIAAGATVTLACPGNEIASLHQVEAEARCL-DNGLLAIDNSEWDLASLGCARPVKETIFRD-LGTCGAEDIGTLHAIGFEIVSLGKYKEIIRVCFEPSSETTLFTEHVIHGANIAAKDIDTSRPSFRTSSGFF-SISMIKAYSQSSQLVLMTNLLGDEDLALSVIDIHKQLYFAKGHMSPDADFVLMANQDASYYYINALPQWQVFNNGNWRNLEYATRDLAEKKGRDLRVISGGWGILELNDINGNPVEIFLGL--IDDKK----VVPAPAITWKVVYDESTNCAAAVVGVNNPFLTTAP---RKLCEDLCSSLSWIDFDVGDLAHGYTYCCSVKDLRASVPHVPDLGDVCLLTD 404          

BLAST of duplex-specific nuclease vs. nr
Match: gi|26892281|gb|AAN86143.1| (duplex-specific nuclease [Paralithodes camtschaticus])

HSP 1 Score: 224.172 bits (570), Expect = 7.535e-65
Identity = 125/361 (34.63%), Postives = 191/361 (52.91%), Query Frame = 0

Query:  112 GQRMLALPWSEPFTVSCPGAYVQLVRERHVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEGRGVRVVIGWLAPSGSLKPQ-IQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFKEGGFYKQTSANQAYKLRTQRETLKSILGP--VVGARIFNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDVGNPEVLEN 469
            G+R++++P     T++C G+ +  +    V   C G G +L + G      +L CS  ++E + R+    CG   +G R  IG+    GS+  + I VC  PVSE T    H L G  I A+++ ++RPS+    GF+   S +  Y   +Q + +  ILG   +    I   +  Y AKGH++PDADF+    Q ATYYF+N  PQWQ+FNNGNWK +E A R+ A   G++L V++G    L+L    G P  + L   +      G +++PVP   WK+VY    ++A   VG+N+P     P     +C D+C+S  W      ++A GYTYCC   D +  I ++PD+GN  +L N
Sbjct:   59 GKRIVSVPSGSSLTLACSGSELINLGMEAVEAKCAG-GVMLAIEGTEWEIWSLGCSNHVKETIRRN-LGTCGEADQGDRHSIGFEYYGGSIYYELISVCFGPVSETTLRTEHVLHGANIAAKDIETSRPSFKTSTGFFS-VSMSTVYSQASQLQLMTDILGDSDLANNIIDPSQQLYFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDINGNPVDILLGLSE------GKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHITTAPS---PLCSDLCSSLTWIDFNLDDLAHGYTYCCAVDDLRQAIPYIPDLGNVGLLTN 407          

BLAST of duplex-specific nuclease vs. nr
Match: gi|260891838|emb|CBG22733.1| (northern shrimp nuclease [Pandalus borealis])

HSP 1 Score: 223.016 bits (567), Expect = 1.968e-64
Identity = 124/359 (34.54%), Postives = 192/359 (53.48%), Query Frame = 0

Query:  113 QRMLALPWSEPFTVSCPGAYVQLVRERHVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEGRGVRVVIGWLAPS-GSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFKEGGFYKQTSANQAYKLRTQRETLKSILGP-VVGARIFN-YKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDVGNPEVLE 468
            QR+ ++       ++CPG  +  +        CLG G L++V G+      L C++   E + R+    CG +  G+  VIG+  P+ G     I+VC DP +E T ++ + + G +I A+++   RPS+    GF+   S    Y  R Q E +K++LG   + A I +  K  Y AKGH+APDADF+    Q ATYY++N  PQWQ+FNNGNWK +E   R+ A   G +L V++G    L+L+   G P  ++L    A D +    ++P P   WK++Y    N+A   VG+N+P     P+    +C DIC+S  W      ++  GYTYCC   D +A I  VPD+G+ ++L+
Sbjct:   57 QRITSVQSGSELILACPGREISALGSEDAQATCLG-GKLVEVDGKEWNIVELGCTKMASETIHRN-LGQCGDQDLGIYEVIGFDLPTTGHFYELIRVCFDPANETTIFSENIVHGASIAAKDIDPGRPSFKTSTGFFS-VSMISVYSQRNQLELMKNLLGDDELAATIIDPSKQFYFAKGHMAPDADFVTVVEQDATYYYINALPQWQAFNNGNWKYLEYDTRDLAEKHGTDLTVYSGGWGVLELEDINGNPVEIYLGL--AQDKK----VVPAPALTWKVIYEKDTNRAAAIVGINNPHITTAPE---PLCTDICSSLTWLDFDFGDLVHGYTYCCSVADLRAAIPNVPDLGDVDILD 403          

BLAST of duplex-specific nuclease vs. nr
Match: gi|1061472071|gb|ODM90863.1| (Nuclease [Orchesella cincta])

HSP 1 Score: 217.624 bits (553), Expect = 1.727e-63
Identity = 116/319 (36.36%), Postives = 176/319 (55.17%), Query Frame = 0

Query:  151 LLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEGRGVRVVIGWLAPSGSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFKEGGFYKQTSANQAYKLRTQRETLKSILGPVVGAR--IFNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDVGNPEVL 467
            +L V+   V +T L CS+ ++E++       C  E   V ++      + +  P I +CH+   + T YA H + G  +   EV++ RPS FKEG FY   SAN AY   +Q+  +  ++G    A   I   ++ Y A+GHLAPD DF++   Q ATYY++NVAPQWQ+ NNGNWKA+E+A+R YA     NL+V+TG  D LKL    G    ++LAR    D +    L P PE  WK++  P  N ++    +N+P     P R + +CPD+C+   W     KN+ KGYTYCC+    +  + ++P++   ++L
Sbjct:    1 MLYVNKNAVNFTQLGCSKQVKEKLVV--VGNCYNESTAVDIL---FQANNTQLPLINICHNTNRDETIYAHHYIIGAGLNPYEVSNNRPS-FKEGQFYTTISANDAYSQSSQKNQVAYLVGSQSLAEKYINTSRSFYFARGHLAPDGDFVHIYEQNATYYYINVAPQWQAINNGNWKALESALRTYAKSKNTNLEVWTGGKDVLKLDDVNGNQVEIYLAR----DSKGKLSL-PAPELSWKVLRDPSRNASVAVFMINNPHLTKIPSR-LIVCPDVCSQISWVTWDVKNVEKGYTYCCKMDSLKNSLPYLPEMSKEQLL 307          

BLAST of duplex-specific nuclease vs. nr
Match: gi|5881881|emb|CAB55635.1| (deoxyribonuclease I [Marsupenaeus japonicus])

HSP 1 Score: 220.32 bits (560), Expect = 2.357e-63
Identity = 126/358 (35.20%), Postives = 189/358 (52.79%), Query Frame = 0

Query:  114 RMLALPWSEPFTVSCPGAYVQLVRERHVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEGRGVRVVIGW-LAPSGSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFKEGGFYKQTSANQAYKLRTQRETLKSILGP--VVGARIFNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDVGNPEVLE 468
            R++ +      T++CPG+ +  +    V V C G G LL V G       L CS+  +E + R+    CG  G GV   IG+ +  S S    I+VC +P +E T Y+ H L G  I A+++ S+RPS+    GF+   S +  Y   +Q  ++K +LG   +  A I  ++  Y AKGHLAPDADF+    Q ATYY++N  PQWQ+FNNGNWK +E A R+ A     +L ++TG    L L    G P  ++L   + +       ++P P   WK+VY    ++A+  VG+N+P     P     +C D+C+S  W      ++  GYTYCC   D +A I  VPD+G+  +L+
Sbjct:   56 RVVRVSAGAKLTLACPGSEIVNLGTTAVDVQC-GGGNLLVVDGTEWRMDELGCSKKDKESIHRN-LGSCGDGGVGVFEGIGFEIFGSDSFYELIRVCFEPKAETTLYSEHVLHGANIAAKDIDSSRPSFKSSTGFFT-VSMSTCYTQNSQLASMKILLGDDDLANAIINPHEQYYFAKGHLAPDADFVTEAEQDATYYYINAVPQWQAFNNGNWKYLEFATRDLAESHSTDLTIYTGGWGVLTLDDINGNPVEIYLGLTEDE------MVVPAPAITWKVVYEESSSRAVGVVGVNNPHITSPP---TPLCSDLCSSLAWIDFDVNDLGHGYTYCCTVDDLRAAIPHVPDLGSVGLLD 401          

BLAST of duplex-specific nuclease vs. nr
Match: gi|1022763209|gb|KZS08846.1| (Uncharacterized protein APZ42_027051 [Daphnia magna])

HSP 1 Score: 217.624 bits (553), Expect = 3.162e-62
Identity = 127/395 (32.15%), Postives = 200/395 (50.63%), Query Frame = 0

Query:   73 PRPDRGCVVDLHDHSVTNLPLFYHPLSEDFLLPQAGEKPGQRMLALPWSEPFTVSC--PGAYVQLVRERHVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEGRGVRVVIGWLAPSGSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFKEGGFYKQTSANQAYKLRTQRETLKSILGP--VVGARIFNYKTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQKNIAKGYTYCCRYQDFQAVIDWVPDVGN 463
            P+   GC  +L   S +N P      + + +LP+       R++ L   +   V C   G Y+Q    +     CL    LL V G+ + ++ L C +  +E +  +    C   G G  + IGW A      P    CHD V+ A +++   + G +  A + ++ RPS F +GG++     N AY    Q +T+  I+G   + G  +      +L++GH+APD DFI    Q ATYYFLN APQWQ+FN  NW  +E A+RN A      L ++TGT D L L    G+ + ++L   + D+  +G  LIPVP+++WKL++ P  N A   +G+N+P  +     ++ ICPD+C    W      +I KGYT+CC   +    I++ PD+G+
Sbjct:   25 PQFKAGCTFNLDSSSPSNPPHLIDA-NNNIILPEM--VGSSRVITLNTDDKVAVGCLGTGNYIQTTGMQLAPATCLSTSKLLLVDGRELSYSQLGCLKQNKEILLEN--GTCA-NGLGTVIRIGWQA-GPEFIPLYDTCHDKVAAANYFSTSFIHGKSAAADDSSNERPS-FSQGGYFPGIDVNNAYSQSQQTQTIADIVGSTSLAGQYVVAGSQIFLSRGHMAPDGDFIDAASQDATYYFLNTAPQWQNFNGANWNVLENALRNLAISRDVKLTIYTGTFDILTLADINGVQQKIYL---EFDENNNG--LIPVPKYYWKLIHDPSTNTATAVLGINNPHIDQVLPADV-ICPDVCNQVPWVTWDLTDIYKGYTFCCTSAELFKAINFAPDLGD 405          

BLAST of duplex-specific nuclease vs. nr
Match: gi|1022766588|gb|KZS11632.1| (Uncharacterized protein APZ42_023548 [Daphnia magna])

HSP 1 Score: 218.394 bits (555), Expect = 2.507e-61
Identity = 131/396 (33.08%), Postives = 209/396 (52.78%), Query Frame = 0

Query:   77 RGCVVDLHDHSVTNLPLFYHPLSEDFLLPQAGEKPGQRMLALPWSEPFTVSCPGAYVQLVRE--RHVSVMCLGEGGLLDVSGQVVPWTNLTCSRSIRERVERDDRAPCGPEGRGVRVVIGWLAPSGSLKPQIQVCHDPVSEATFYARHTLFGGAIGAREVASARPSYFKEGGFYKQTSANQAYKLRTQRETLKSILGP-VVGARIFNY-KTSYLAKGHLAPDADFIYREWQRATYYFLNVAPQWQSFNNGNWKAVEAAVRNYASLTGANLDVFTGTLDPLKLQSDAGIPKALHLARKDADDPQSGFDLIPVPEFFWKLVYHPGVNQAIVFVGLNDPFFEGNPDREMQICPDICASQKWFFLYQK-NIAKGYTYCCRYQDFQAVIDWVPDVGNPEVL 467
            R C  +L D S    P      S+  + P    + G R + L  ++  TV+C G+   L     +  S  C   G  L V  Q + + +L C+  ++E ++  +   C     G ++ IGW   S +   QI +CH   ++ T YA  T++G  + A +  + RPS F++G +Y     + AY    Q  T+  I+G   +GA+  +  K+ Y A+GHLAPDADFI    Q ATYY++N APQWQSFNNGNWKA+EA  RN A     +L V+TGT + L L +  G+ K ++LA+   ++     +++PVP+++WK+V+ P  N+A  F+G+N+P        ++  C D+C+   W     + +I KGY +CC       V+   P++G+  +L
Sbjct:  146 RTCTFNL-DTSSPTYPSHLVDSSDQIVQPTL--QNGVRTITLAANQLVTVACEGSGNALTATGVQFNSGKCASSGTSLQVGTQNLGYGSLGCNSVVKETIK--ETGTC--SSTGTKIEIGWQV-STTFINQITICHVESNDNTLYAIDTIYGANVAADDKQNERPS-FRKGNYYTGVDVDTAYSQDGQLITVSEIVGSSTLGAQYIDVSKSYYFARGHLAPDADFIDAGSQDATYYYINAAPQWQSFNNGNWKALEAGSRNLAISRNIDLVVYTGTYEVLTLANVNGVQKPIYLAQDTNNN-----NVLPVPKYYWKVVHDPATNKATAFIGINNPHLSSVSGADV-FCSDVCSQIGWMSWTNRFDIPKGYMFCCTAAALHNVVPHAPNLGSIGLL 526