hypothetical protein, snap_masked-scaffold5739_size4378-processed-gene-0.0 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000004194 (supercontig:LSalAtl2s:LSalAtl2s221:469662:476711:-1 gene:EMLSAG00000004194 transcript:EMLSAT00000004194 description:"augustus_masked-LSalAtl2s221-processed-gene-4.2") HSP 1 Score: 438.343 bits (1126), Expect = 3.459e-129 Identity = 266/751 (35.42%), Postives = 405/751 (53.93%), Query Frame = 0
Query: 10 PDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPADIGVGVFFLPEGISANVKSFTE--SVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTE-VDAAHFERLLFLVRREVEREF----TKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSG-RSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLL-------------------------------YIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSF----TSHAALVPT-----------EDF-NALDLSRQQVTH------------GVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDK-IEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYL 692
P + G L++ + E CG+G I N+ G S+DI + A M HRGA AD +GDG+GVL IP + K + P G+ F E ++VK E + + N+ + WREVP + + LG +AR ++P++ + V+ E ++ F++ ++++R+ FYI +LS I YKG P + +++DL D +F++ + + H RFSTNTFP+W P R++ HNGEINT+RGN N M +RE S + +D+ L ++ SDS LD+ +E LV G RSL AM LVP A++ND ++ + FY++ + EPWDGPA L FTDGR + A LDRNGLRPSR+ +T++ + SE G I + ++II++GRL PG++L DT + + D E+K +A +P+ +++++ ++LK + +AA + + DF N D + + G E L+M+ PM K +EA+ SMG+D PLA LS L++ YFKQLFAQVTNPPIDP RE VM+L +G E N+L P L L++ IL +LE + G+ S VID T+ S+ + L + IC AE AV + +++LILSDR +R+P+ + LA+G+VHHHL R R + ++++++GEAR+ H + L GFGA A+CPY+
Sbjct: 11 PSAEG-LYDPALEKDACGVGFIVNIDGRTSHDIFEDAAELSRRMEHRGACSADNDSGDGAGVLIAIPKDYYQKKLSEVNVQL-PRLYATGIIFCDESDESDVKKDFEKHAESLKGNLRVIHWREVPKDSNSLGSVARASEPKLLQVFVTSEEEGIENKCFDKYIYILRKTATHYIPACKDSRKRFYICSLSRTAIVYKGQFNPCQIWNYFTDLMDPEFESHLLIVHTRFSTNTFPSWERAHPNRVLAHNGEINTLRGNVNLMEAREGVMVSPDF-EDLSTLYPIVEPDMSDSGCLDNVVEFLVHVGDRSLPEAMMTLVPEAWQNDDQMPSNKKNFYHWASFYMEPWDGPALLTFTDGRYVGAILDRNGLRPSRYYITKNNRMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASEVGVIDIDPTQIIKKGRLQPGKILLVDTDVQKVIDDHELKSSIAXSRPHGDFLNKS-IDLKIIKEAGSEYAAEISSLPRRRPINLHENDFNNNFDYGEENILEKLWRGDRRLPIFGYNIETLEMLMLPMFKTKKEALGSMGNDTPLACLSEMQPLIYEYFKQLFAQVTNPPIDPFREKIVMSLECPIGPEGNIL--NPGDCTRLWLKNPILSVNDLEHLKRTSILGWKSRVIDITY-LSDDQISLSDIVSNICNRAEEAVKKEGVQLLILSDRNVGVNRIPVSAALASGAVHHHLIATRLRSKCAIIVESGEAREVHHLCVLIGFGADAICPYM 754 HSP 2 Score: 383.259 bits (983), Expect = 2.360e-110 Identity = 222/504 (44.05%), Postives = 292/504 (57.94%), Query Frame = 0
Query: 838 KEDYDDYVKVTIENDPITIKDIL---AFKV---PAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPA-NSIDLTFRGSAGQSFGTFLCGGILSTV 1334
K Y YV T+E TIK F++ V + EVE IVKRF T AMS G++S EAH TLA+AMN+LGGKS++GEGGE+ R+ D RS IKQ+ASGRFGVSA YL +ADEL+IKMAQGAKPGEGG+LPG+KV IA+ R + P A+++VKLV+E GVG +AAG AK A++ILISGHDGGT AS + K AG+PWELGI+E HQ L+MNNLR+ V L+TDG LRTG DV++A ILGA++ F T +I +GC +RKCHLN CPVGIAT P R KF+G P+HVV Y +AEE RE MA LG N+L+G + FL+ E P K + L+ +++L +++RI H ++ + +L + + P + Y +VNT R G + +A Y GLP N I+++ GS G++F + GGI V
Sbjct: 775 KNAYQKYVDATME----TIKKCSIRGQFEIDFSKGTPVDVSEVELASNIVKRFVTGAMSFGSISAEAHGTLAVAMNKLGGKSNTGEGGENEERYL----NDIRRSAIKQVASGRFGVSASYLAHADELQIKMAQGAKPGEGGELPGYKVTVEIAKTRRSVP--------------------------------ARISVKLVSEVGVGVIAAGVAKGKAEHILISGHDGGTGASSWTGIKGAGLPWELGIAETHQTLVMNNLRSRVVLQTDGQLRTGFDVMVAGILGADEFGFSTAPLIVLGCTMMRKCHLNTCPVGIATQDPILRKKFEGQPEHVVNYFFLIAEEIREFMAKLGCTKFNDLIGHTE-FLKVTENPIPSKTHLLNFNSLLHSA---ESMRIPKK------HGGSIPQDFQLNLRLDNEVIKACDTIINSDSLNPISLEYPIVNTQRTFGATLSYHIAMKYNESGLPRDNIINISLHGSGGRNFAAGMSGGIAYVV 1228
BLAST of hypothetical protein vs. SwissProt
Match: gi|1707983|sp|P51375.1|GLTB_PORPU (RecName: Full=Ferredoxin-dependent glutamate synthase; AltName: Full=Fd-GOGAT) HSP 1 Score: 1129.39 bits (2920), Expect = 0.000e+0 Identity = 616/1357 (45.39%), Postives = 831/1357 (61.24%), Query Frame = 0
Query: 13 SGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPAD-IGVGVFFLPEGISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFT---KEAA--FYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISE--EVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDR--ATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASD------VKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAF-QVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFK--------------PY----PNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDAIRICTH--ERNDGFHK--PALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
S +L E CG+G IA++ ++ I+ AL ++ M HRGA AD+ +GDG+G+ IP LF + G + IGVG+ FLP K E+VL N+ +GWR VP + LG A L +P + + R + + E+ LFLVR+++ER KE A FY+ +LS I YKG+ A L FY DL S++ + ++YH+RFSTNT P WPL QP R + HNGEINT+ GN NWM SRE +S +W + I L+ + + SDSA+LD A+ELL+ SGRS E A+ +LVP AF+N E ++ E+ FY Y EPWDGPA + FTDG+ I A LDRNGLRP+R+ +T+D L+ + SESG + + S I +GRLGPGQM+S D + + L +KEIK +A + PY + E+R H A + + L + Q G T ED+++V M +E F MGDDIPLA LS +L+ YFKQ FAQVTNP IDP+RE VM+L +G + NLL + P A+ + L+S I+ E EL I K S I+T + +G KK ++++C A A+ ILILSD+ +++V IP LLA G+VHHHL R S++I+T + TH ACL G+GA+A+CPYL + T R ++ KG+L + ANY+KA+E GLLKI+SKMGIS+L+SY GAQIFE LG+G EVVNFAF G S+IGG+ E+ +E++ H AF QV KT L + G+ +YR GE H ++ K LH VR E Y+ Y ++ P ++D+L K +A+++VE +E I+ +F T MS+GALS E HETLAIAMNR+GGKS+SGEGGED RFK P+ NGD A S IKQIASGRFGV+ YL+NA +LEIK+AQGAKPGEGGQLPG K++ IA LR +PGV LISPPPHHDIYSIEDL+QLI DL ++NP AK++VKLV+E G+GT+AAG AK +AD I ISGHDGGT ASPLSS KHAG PWELG+SE HQ+L N LR+ VTLR DGGLRTG D+++AAI+GAE+ FGTIAMIA GC+ R CH N CPVG+AT + RA+F G P+ +V + + + E REI+ASLG +S++E+ G+ ++ ++ + K ++L+++ I I TH + + H P +D +ILA + E A + +N A K + + NT+R +GTR +G +A+ Y N G I L F GSAGQSFG FL G+
Sbjct: 21 SSSLVSIEKERDACGVGFIADVNNIANHKIVVQALEALTCMEHRGACSADRDSGDGAGITTAIPWNLFQSGLKDKGIIIQKNESIGVGMLFLPTSKLQESKKIIENVLKEENLEVVGWRLVPTVEEVLGKQAYLNKPHVEQMFC-RSSNLSKNELEQQLFLVRKKIERYIGINGKEWAHEFYVCSLSCYTIVYKGMMRSAVLGQFYQDLYHSEYTSSFAIYHRRFSTNTMPKWPLAQPMRFISHNGEINTLLGNLNWMRSREPLLKSPIWKNRIDELKPITNKDNSDSANLDAAVELLIASGRSAEEALMILVPEAFQNQPEFNKNTEISDFYEYYSGLQEPWDGPALVVFTDGKVIGATLDRNGLRPARYVITKDNLVIVSSESGVVQVEPSNIKSKGRLGPGQMISVDIISHKILNNKEIKTSVAGKTPYGDLLKESR----QILGHQAFFSEQQVESKKLMQLQTAFGYTNEDVELVIEHMASQGKEPTFCMGDDIPLAILSEKSHILYDYFKQRFAQVTNPAIDPLRESLVMSLTIQIGHKSNLLDDQPVLAKHIKLDSPIINEGELNAILESK---LSCAHINTIFKVEKGPNDFKKQIEQLCESASQAILSGNNILILSDKNDILESEKVSIPPLLAAGAVHHHLINKGLRQDASIIIETAQCWSTHHFACLIGYGASAICPYLAFETARHWWSNPKTKMLMSKGRL-PACNIQEAQANYKKAVEAGLLKILSKMGISLLSSYHGAQIFEILGLGSEVVNFAFKGTTSQIGGLSMEELGQETVNIHSKAFSQV-----KTKKLANYGFVQYRPGGEYHINNPEMSKALHQAVRGYNPEYYNSYQRLLQNRPPTALRDLLKLKSNKQPIAIDKVESMENILHKFCTGGMSLGALSRETHETLAIAMNRIGGKSNSGEGGEDPVRFKVLNDVNESGNSDLLPHLKGLRNGDTASSAIKQIASGRFGVTPEYLMNAKQLEIKIAQGAKPGEGGQLPGKKISPYIATLRKCKPGVPLISPPPHHDIYSIEDLSQLIFDLHQINPTAKISVKLVSEIGIGTIAAGVAKGNADIIQISGHDGGTGASPLSSIKHAGSPWELGLSEVHQLLAENQLRDRVTLRVDGGLRTGSDIVLAAIMGAEEFGFGTIAMIATGCIMARICHTNKCPVGVATQREELRARFSGVPEALVNFFLFIGNEVREILASLGYKSLDEITGQNHLLIKNTDI-ELAKTRGIELNSL---------ININTHTWTKFNSVHTNGPVMDDDILA-IPEINDAIKLENEVA---------KHFKIANTNRTVGTRLSGVIAQKYGNEGFKG-LIKLNFYGSAGQSFGAFLASGV 1342
BLAST of hypothetical protein vs. SwissProt
Match: gi|2506463|sp|P55038.2|GLTS_SYNY3 (RecName: Full=Ferredoxin-dependent glutamate synthase 2; AltName: Full=FD-GOGAT) HSP 1 Score: 1127.85 bits (2916), Expect = 0.000e+0 Identity = 620/1351 (45.89%), Postives = 837/1351 (61.95%), Query Frame = 0
Query: 26 CGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPAD-IGVGVFFLPEGISAN--VKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGD-DIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISE--EVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETI-TNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSA----DRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKLG-----DDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFV---------RDGKKEDYDDYVKVTIENDPIT-IKDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFK--------------PY----PNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDAIRICTHE--RNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
CG+G IANLRG+ + +++ AL ++ M HRG AD +GDG+GV+ IP L A+ D +GVG+ FLP+ SA +++ E V+ + LGWREVPVN D LG A+ QP I +LV+ P +R L++ R + ++ ++ FY+ + S R I YKG+ L FY DL++ + + ++YH+RFSTNT P WPL QP R++ HNGEINT+ GN NWMA+RE+ E W ++ L ++ + SDS +LD ALELLV +GRS A +LVP A++N + + E+ F++Y EPWDGPA L F+DG+ + AGLDRNGLRP+R+ +T+D + +GSE+G + LP+ I+ +GRL PGQM++ D + L + +IK++ A + PY W+ +++ ++ S + T +A + +QQ G TAED++MV PM +E F MGDD PLA LS PRLL+ YFKQ FAQVTNPPIDP+RE VM+L LG NLL E AR + L S ++ E EL+ I T + + S + D +G L+ ALD + A V EIL+L+DR A ++ IP LLA G+VHHHL R RL+ SL++DT + TH ACL G+GA+A+CPYL +VRQ + KL D I + NYR+++E GL KI+SKMGIS+L SY GAQIFEA+G+G E+V +AF G SR+GG+ A++A E +V H AF P L + G+ YR GE H + ++ K+LH V +G+ D+ + + +++ P+T ++D+L F ++LEEVE VE IVKRF T MS+GALS EAHETLAIAMNRLG KS+SGEGGED R+ P+ NGD A S IKQIASGRFGV+ YL++ +LEIKMAQGAKPGEGGQLPG KV+ IA LR ++PGV LISPPPHHDIYSIEDLAQLI+DL ++NP A+V+VKLVAE G+GT+AAG AKA+AD I ISGHDGGT ASPLSS KHAG PWELG++E H+VLM N LR+ V LR DGGL+TG DV++AA++GAE+ FG+IAMIA GC+ R CH NNCPVG+AT + R +FKG P VV + +AEE R ++A LG RS+++++GR L+ R K L L +L + HE ++G P LD +ILAD A TA TK+Y +VNTDR +GTR +G +A+ Y N+G N I L F+G+AGQSFG F G+
Sbjct: 37 CGVGFIANLRGKPDHTLVEQALKALGCMEHRGGCSADNDSGDGAGVMTAIPRELLAQWFNTRNLPMPDGDRLGVGMVFLPQEPSAREVARAYVEEVVRLEKLTVLGWREVPVNSDVLGIQAKNNQPHIEQILVTCPEGCAGDELDRRLYIARSIIGKKLAED--FYVCSFSCRTIVYKGMVRSIILGEFYLDLKNPGYTSNFAVYHRRFSTNTMPKWPLAQPMRLLGHNGEINTLLGNINWMAAREKELEVSGWTKAELEALTPIVNQANSDSYNLDSALELLVRTGRSPLEAAMILVPEAYKNQPALKDYPEISDFHDYYSGLQEPWDGPALLVFSDGKIVGAGLDRNGLRPARYCITKDDYIVLGSEAGVVDLPEVDIVEKGRLAPGQMIAVDLAEQKILKNYQIKQQAAQKYPYGEWI---KIQRQTVASDSFAEKTLFNDAQTVLQQQAAFGYTAEDVEMVVVPMASQGKEPTFCMGDDTPLAVLSHKPRLLYDYFKQRFAQVTNPPIDPLRENLVMSLAMFLGKRGNLLEPKAESARTIKLRSPLVNEVELQAIKTGQLQVAEVSTLYDL-----DGVNSLETALDNLVKTAIATVQAGAEILVLTDRPNGAILTENQSFIPPLLAVGAVHHHLIRAGLRLKASLIVDTAQCWSTHHFACLVGYGASAICPYLALESVRQWWLDEKTQKLMENGRLDRIDLPTALKNYRQSVEAGLFKILSKMGISLLASYHGAQIFEAIGLGAELVEYAFAGTTSRVGGLTIADVAGEVMVFHGMAF-----PEMAKKLENFGFVNYRPGGEYHMNSPEMSKSLHKAVAAYKVGGNGNNGEAYDHYELYRQYLKDRPVTALRDLLDFNADQPAISLEEVESVESIVKRFCTGGMSLGALSREAHETLAIAMNRLGAKSNSGEGGEDVVRYLTLDDVDSEGNSPTLPHLHGLQNGDTANSAIKQIASGRFGVTPEYLMSGKQLEIKMAQGAKPGEGGQLPGKKVSEYIAMLRRSKPGVTLISPPPHHDIYSIEDLAQLIYDLHQINPEAQVSVKLVAEIGIGTIAAGVAKANADIIQISGHDGGTGASPLSSIKHAGSPWELGVTEVHRVLMENQLRDRVLLRADGGLKTGWDVVMAALMGAEEYGFGSIAMIAEGCIMARVCHTNNCPVGVATQQERLRQRFKGVPGQVVNFFYFIAEEVRSLLAHLGYRSLDDIIGR-TDLLKVRSDVQLSKTQNLTLDCLLNLPDTKQNRQWLNHEPVHSNG---PVLDDDILAD--PDIQEAINHQTTA--------TKTYRLVNTDRTVGTRLSGAIAKKYGNNGFEGN-ITLNFQGAAGQSFGAFNLDGM 1357
BLAST of hypothetical protein vs. SwissProt
Match: gi|122194649|sp|Q1XDB2.1|GLTB_PYRYE (RecName: Full=Ferredoxin-dependent glutamate synthase; AltName: Full=Fd-GOGAT) HSP 1 Score: 1127.46 bits (2915), Expect = 0.000e+0 Identity = 607/1355 (44.80%), Postives = 833/1355 (61.48%), Query Frame = 0
Query: 12 SSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPAD-IGVGVFFLPEGISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFT---KEAA--FYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISE--EVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSAD--RVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASD------VKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFK--------------PY----PNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDAIRICTHERNDGFHK--PALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
+S +L E CG+G IA++ ++ I+ AL ++ M HRGA AD+ +GDG+G+ IP LF K+ N + D +GVG+ FLP K E+VL N+ +GWR VP + LG A L +P + + + + + E+ LFLVR+++E+ K+ A FYI +LS I YKG+ A L FY DL S++ + ++YH+RFSTNT P WPL QP R + HNGEINT+ GN NWM SRE +S+VW D IH L+ + + SDSA+LD A+ELL+ SGRS E A+ +LVP AF+N + + E+ FY Y EPWDGPA + FT+G+ I A LDRNGLRP+R+ +T+D L+ + SESG + + + +GRLGPGQM+S D + + L +KEIK + + PY + + R L+ H + + + L + Q G T ED+++V M A+E F MGDDIPL+ LS +L+ YFKQ FAQVTNP IDP+RE VM+L +G + NLL + P A+ + LES ++ E EL I K S + I+T + +G K+ + ++C A A+ D IL+LSD+ S D +V IP LLA G+VHHHL R S++++T + TH ACL G+GA+A+CPYL + T R ++ KG+L E ANY+KA+E GLLKI+SKMGIS+L+SY GAQIFE LG+G EVVN AF G S+IGG+ E+ E++ H AF KT L + G+ +YR GE H ++ K LH VR E Y++Y + P ++D+L + A ++++EVE +E+I+++F T MS+GALS E HETLAIAMNR+GGKS+SGEGGED RFK P+ NGD A S IKQIASGRFGV+ YL+NA +LEIK+AQGAKPGEGGQLPG K++ IA LR +PGV LISPPPHHDIYSIEDL+QLI DL ++NP+AK++VKLV+E G+GT+AAG AK +AD I ISGHDGGT ASPLSS KHAG PWELG+SE HQ+L N LR+ VTLR DGGLRTG D+++AAI+GAE+ FGT+AMIA GC+ R CH N CPVG+AT + RA+F G P+ +V + + + E REI+ASLG +S++++ G+ ++ ++ + K N + L ++ I T + + H P +D +ILA + E + A + + TK + + NT+R +GTR +G +A+ Y N G I L F GSAGQSFG FL GI
Sbjct: 20 NSSSLISIEKERDACGVGFIADVNNVANHKIVVQALEALTCMEHRGACSADRDSGDGAGITTAIPWNLFQKSLQNQNIKFEQNDSVGVGMLFLPAHKLKESKLIIETVLKEENLEIIGWRLVPTVQEVLGKQAYLNKPHVEQVFC-KSSNLSKDRLEQQLFLVRKKIEKYIGINGKDWAHEFYICSLSCYTIVYKGMMRSAVLGQFYQDLYHSEYTSSFAIYHRRFSTNTMPKWPLAQPMRFVSHNGEINTLLGNLNWMQSREPLLQSKVWKDRIHELKPITNKDNSDSANLDAAVELLIASGRSPEEALMILVPEAFQNQPDFANNTEISDFYEYYSGLQEPWDGPALVVFTNGKVIGATLDRNGLRPARYVITKDNLVIVSSESGVVQVEPGNVKSKGRLGPGQMISVDIFSHKILNNKEIKTSVTTKIPYGELLTDARQILE----HKPFLSDQQVDIKKLMQLQTAFGYTNEDVELVIEHMASQAKEPTFCMGDDIPLSILSEKSHILYDYFKQRFAQVTNPAIDPLRESLVMSLAIQIGHKSNLLDDQPTLAKHIKLESPVINEGELNAIFESK---LSCIRINTLFQLEDGPKNFKQQIQQLCENASQAILDGNNILVLSDKNNSLDSEKVSIPPLLAVGAVHHHLINKGLRQEASILVETAQCWSTHHFACLIGYGASAICPYLAFETARHWWSNPKTKMLMSKGRLPACNIQE-AQANYKKAVEAGLLKILSKMGISLLSSYHGAQIFEILGLGSEVVNLAFKGTTSQIGGLSMIELGRETVNIHSKAF----SEVKTKKLANYGFVQYRPGGEYHINNPEMSKALHQAVRGYNPEYYNNYQSLLQNRPPTALRDLLKLQSNRAPISIDEVESIEDILQKFCTGGMSLGALSRETHETLAIAMNRIGGKSNSGEGGEDPVRFKILNDVNSSGTSPLLPHLKGLKNGDTASSAIKQIASGRFGVTPEYLMNAKQLEIKIAQGAKPGEGGQLPGKKISPYIATLRKCKPGVPLISPPPHHDIYSIEDLSQLIFDLHQINPKAKISVKLVSEIGIGTIAAGVAKGNADIIQISGHDGGTGASPLSSIKHAGSPWELGLSEVHQLLAENQLRDRVTLRVDGGLRTGSDIVLAAIMGAEEFGFGTVAMIATGCIMARICHTNKCPVGVATQREELRARFSGVPEALVNFFLFIGNEVREILASLGYKSLDDITGQNHLLIKNTDI-NLTKTNGIQLDTLI-------NINNNTWTKFNSVHTNGPVMDDDILA-IPEVSNAIKLET---------EITKHFKIANTNRTVGTRLSGIIAKNYGNTGFKG-LIKLNFYGSAGQSFGAFLASGI 1342
BLAST of hypothetical protein vs. SwissProt
Match: gi|1707984|sp|P55037.1|GLTB_SYNY3 (RecName: Full=Ferredoxin-dependent glutamate synthase 1; AltName: Full=Fd-GOGAT) HSP 1 Score: 1110.9 bits (2872), Expect = 0.000e+0 Identity = 584/1328 (43.98%), Postives = 840/1328 (63.25%), Query Frame = 0
Query: 16 LHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFT-GDPADIGVG-VFFLPEGIS-ANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEV-DAAHFERLLFLVRREVE---REFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDP--ITIKDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFK-PYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLAD-KAGDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENH--GLPANSIDLTFRGSAGQSFGTFLCGGI 1330
L++ NE CG+G I ++G+ S+DI++ L + N+ HRGA + TGDG+G+L Q+PH K A G T P VG ++ P+ ++ A + ++ + + LGWR++P + LG+ A ++P + + ++RP + D FER L+++R+ R + +Y+ +LS+R + YKG+ A + +Y +L D D ++ ++L H RFSTNTFP+W P+R + HNGEINT+RGN NWM +R+ FES ++G+D+ ++ ++ SDS D+ALELL L+GRSL HA+ M++P + + +S+E +AFY Y EPWDGPA + FT+G+ + A LDRNGLRPSR+ +T+D L+ + SE+G + + ++ ++GRL PG+M D + G + D+EIK+ + +Q PY W+ N L+ S VP D A L ++Q+ G T E+L ++ PM + EA+ SMG D PLA LS P+LL+ YF+QLFAQVTNPPID IRE + + +G E NLL PE R++ L++ IL ++L + + F S+ +D + ++GEAGLK ALD + EA+ A++ ++ILSDR SA++ IP+LLA +HHHL R R + LV+++GE R+ H A L G+G A+ PYL + T+ ++A L ++ + NY KA KG++K+ SK+GIS + SY+GAQIFEA+G+ + V++ F SRI G IA+E+++RH+ AF TLD+G G ++RK GE H + I L VR+G E Y Y + E + T++ +L F+ + LEEVEP+E I+KRF T AMS G++S EAHE+LAIAMNR+GGKS++GEGGED RF GD S IKQ+ASGRFGV++ YL A E++IKMAQGAKPGEGGQLPG KV IA++R++ PGV LISPPPHHDIYSIEDLA+LIHDLK N A++ VKLV+E GVGT+AAG AKA AD +L+SG+DGGT ASP +S KHAG+PWELG++E HQ L++NNLR+ + + TDG ++TGRDV IAA+LGAE+ F T ++++GC+ +R CHLN CPVGIAT +P+ RAKF G P H V +M +A E RE+MA LG R+INE+VGR L+ ++ H KA +DLS +L + GDD R C ++ G +LD+ L DL + A A+G+ TA L + N +R +GT EI + H GLP +++ L F+GSAGQSFG F+ G+
Sbjct: 33 LYDPQNEHDACGVGFIVQMKGKVSHDIVEQGLQMLVNLEHRGACGCEPNTGDGAGILIQVPHKFIQKIAGAEGITIPAPGQYAVGNIYGSPDPLARAEARQKFNDIVAQEGLKVLGWRDIPTQNEPLGETAIASEPFMQQVYIARPEGLTDDLDFERKLYVIRKLTHGAIRSPKIDTYWYVASLSARTLVYKGMLTTAQVGQYYPELHDPDMESALALVHSRFSTNTFPSWERSHPYRYIAHNGEINTMRGNVNWMQARQALFESSLFGEDMAKVQPVINIDGSDSTIFDNALELLYLAGRSLPHAVMMMIPEPWSAHESMSQEKKAFYKYHSCLMEPWDGPASIAFTNGKMMGAVLDRNGLRPSRYYVTKDDLVIMASEAGVLPIEPERVAKKGRLQPGRMFLVDMEQGRIIADEEIKQEIVSQHPYGEWLAANLKSLEQLPSPGN-VPGTD--AESLRQRQMAFGYTFEELRILLAPMGRDGVEAIGSMGADTPLAVLSDKPKLLYNYFQQLFAQVTNPPIDSIREEIITSAETTIGGEGNLLDPRPESCRLIELKTPILTNEDLAKLKALDDDEFKSVTLDILFDPNQGEAGLKTALDNLFTEADQAISQGANLIILSDRQVSAEKAAIPALLAVSGLHHHLIRNGSRTKVGLVLESGEPREVHHFAVLLGYGCGAINPYLAFETLDGMIAEG----LLVNVDHKTACKNYIKAATKGVIKVASKIGISTIQSYRGAQIFEAVGLNQSVIDEYFCRTSSRIQGSDLGVIAQEAILRHQHAFAPRPGDLHTLDVG--GEYQWRKDGEEHLFSPQTIHLLQRAVREGNYELYKQYAALVNEQNQKFFTLRGLLDFQ-DRESIPLEEVEPIEAIMKRFKTGAMSYGSISKEAHESLAIAMNRIGGKSNTGEGGEDPERFTWTNDQGDSKNSAIKQVASGRFGVTSLYLSQAKEIQIKMAQGAKPGEGGQLPGKKVYPWIAKVRHSTPGVGLISPPPHHDIYSIEDLAELIHDLKNANREARINVKLVSEVGVGTIAAGVAKAHADVVLVSGYDGGTGASPQTSIKHAGLPWELGLAETHQTLVLNNLRSRIVVETDGQMKTGRDVAIAALLGAEEFGFSTAPLVSLGCIMMRACHLNTCPVGIATQNPELRAKFTGDPAHAVNFMTFIATELREVMAQLGFRTINEMVGR-TDILEPKKAVAHWKAKGIDLSTILHQPEVGDDVGRYCQIPQDHGLQH-SLDITQLLDLCQ-PAIAKGEKVTATL----------PITNINRVVGTIVGN---EITKRHWEGLPEDTVHLHFQGSAGQSFGAFIPKGM 1334
BLAST of hypothetical protein vs. SwissProt
Match: gi|239938894|sp|P39812.3|GLTA_BACSU (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=NADPH-GOGAT) HSP 1 Score: 1104.35 bits (2855), Expect = 0.000e+0 Identity = 590/1330 (44.36%), Postives = 819/1330 (61.58%), Query Frame = 0
Query: 7 DHTPDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPADIGVGVFFL--PEGISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEV-DAAHFERLLFLVRREVEREFTKEAA-FYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYN-RYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDAIRICTHERNDGFHKPALDLEILADL-AEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
+ P + G L+ E CG+G A+L+G++++DI+ L +C + HRG +D TGDG+G+L QIP A F K N + GVG+ F E ++ +++ + LGWR VPVN ++G +A+ + P + + + +++ D FER L+++R++ E E FY +LSS+ I YKGL P + AFYSDLQD F + +L H RFSTNTFP W P R + HNGEINT+RGN NWM +RE+ F SE +G+D++ + ++ SDS+ LD+A E V++GR H ML+P + + +S+E RAFY Y S EPWDGP + FTDG++I A LDRNGLRP+R+ +T+D + SE G I + ++ + RL PG+ML D + G + D+E+K ++A + PY +W++E EL E F+ DL +Q G T ED+ P+IK ++ + SMG+D PLA LS + LF YFKQLFAQVTNPPID IRE V + LGAE +LL + + R + L + +L ++ + S ID + L++ L + +AE A++ + +LILSD+ + PIP LLA ++H HL R R + S+++++GEAR+ H A L G+GA A+ PYL Y+T +Q + +G+L DI+ E ++ Y K++ +G++K+MSKMGIS + SY+GAQIFEA+G+ ++V++ F+G S++GG+ IAEE+ RHR A+Q D KTL+ PG + ++R GE HA I L R + Y K E ++++ AF + LEEVE E IVKRF T AMS G+LS EAHE LAIAMNRLGGKS+SGEGGED +RF P NGD RS IKQIASGRFGV +HYLVNADEL+IKMAQGAKPGEGGQLPG+KV +A +R + PGV LISPPPHHDIYSIEDLAQLIHDLK N A+++VKLV+++GVGT+AAG AKA+AD I+ISG+DGGT ASP +S KH G+PWELG++EAHQ LM+N LR+ V L TDG L TGRDV++AA+LGAE+ F T ++ +GCV +R CHL+ CPVG+AT +P+ R KF G PDH+V YM+ +AEE RE MA+LG ++ +E++GR +H KA+ LDLS +L G +R +N HK L+I L A A G+ S + NT+R GT T +++ Y GLP ++I L F GSAGQSFG F+ G+
Sbjct: 4 NQMPKAQG-LYRPEFEHDACGIGLYAHLKGKQTHDIVKQGLKMLCQLDHRGGQGSDPDTGDGAGLLVQIPDAFFRKECKNINLP-EKERYGVGMVFFSQKEDERKKIEKQINALIEQEGQVVLGWRTVPVNVGKIGTVAQKSCPFVRQVFIGASSDLKDNLSFERKLYVIRKQAENWGVTEGLDFYFASLSSQTIVYKGLLTPEQVDAFYSDLQDEAFVSAFALVHSRFSTNTFPTWERAHPNRYLVHNGEINTLRGNINWMRAREQQFVSESFGEDLNKILPILNADGSDSSILDNAFEFFVMAGRKPAHTAMMLIPEPWTENTHMSKEKRAFYEYHSSLMEPWDGPTAISFTDGKQIGAILDRNGLRPARYYVTKDDYIIFSSEVGVIEVEQENVLYKNRLEPGKMLLIDLEEGRIISDEEVKTQIATEYPYQKWLEE---ELVQVNPDPESREEEQFS--DLLTRQKAFGYTYEDIQKYLIPVIKEGKDPLGSMGNDAPLAVLSDRAQSLFNYFKQLFAQVTNPPIDAIREQLVTSTMTWLGAEGDLLHPSERNVRRIKLYTPVLSNEQFYALKTIVHPDLKSQKIDVLFSED-----LERGLKDMFTQAEKAISQGVSLLILSDKKMNERLTPIPPLLAVSALHQHLIRKGLRTKVSIIVESGEAREVHHFAALIGYGADAINPYLAYATYKQEID---EGRL--DISYEEAVSKYGKSITEGVVKVMSKMGISTVQSYRGAQIFEAVGISRDVIDRYFSGTASQLGGIDLQTIAEEAQRRHREAYQDDY--SKTLE---PGSDFQWRNGGEHHAFNPKTIHTLQWACRRNDYNLFKQYTKAADEERIGFLRNLFAFDGNRKPLKLEEVESAESIVKRFKTGAMSFGSLSKEAHEALAIAMNRLGGKSNSGEGGEDPKRFVPDENGDDRRSAIKQIASGRFGVKSHYLVNADELQIKMAQGAKPGEGGQLPGNKVYPWVADVRGSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANRDARISVKLVSKAGVGTIAAGVAKATADVIVISGYDGGTGASPKTSIKHTGLPWELGLAEAHQTLMLNGLRDRVVLETDGKLMTGRDVVMAALLGAEEFGFATAPLVVLGCVMMRACHLDTCPVGVATQNPELRKKFMGDPDHIVNYMLFIAEEVREYMAALGFKTFDEMIGRTDVLHASERAKEHWKASQLDLSTLLYQPEG---VRTFQSPQN---HKIDQSLDITTILPAVQEAIESGKEADI----------SIEINNTNRVAGTITGSEISKRYGEEGLPEDTIKLHFTGSAGQSFGAFVPKGM 1295
BLAST of hypothetical protein vs. SwissProt
Match: gi|1179881379|sp|E1V8I1.1|GLTB_HALED (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=GOGAT; AltName: Full=Glutamate synthase subunit alpha; Short=GLTS alpha chain) HSP 1 Score: 1102.81 bits (2851), Expect = 0.000e+0 Identity = 609/1320 (46.14%), Postives = 812/1320 (61.52%), Query Frame = 0
Query: 16 LHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPADIGVGVFFLPE--GISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKLGDDI-TPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAK-FKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLA-DKAGDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
LH+ NCG G IA++ G+ S+D++ A+ S+ MTHRG + AD KTGDG G+L ++P + + A VG FLP+ A + E+ LT+R + LGWREVPV+P G +A P I L V P E F+ LF+ RR E+ E FY+ +LS ++SYKGL MP L AFY DL D+ +T I ++HQRFSTNT P WPL QPFR++ HNGEINT++ NR W SR+E F SE DI L ++ SDS+S+D+ LE+L+ G L A+ M+VPPA++N + + ++RAFY Y +EPWDGPAG+ TDGR+ LDRNGLRP+R+ +T +G + + SE G ++ +GR+GPGQ+L+ DT+TGE L+ ++I RL + PY RW+ + L+S + L ++ +A L+ QQ ++ E+ D + P+ + QE V SMGDD P+A LS RLL YF+Q FAQVTNP IDP+RE VM+L + +GAE N+ TPEHA L L + +L ++ + +++ F+S + + GLK AL +CAEAE A +L+LSD ++PI + LA G+VHHHL R R R +L+++TG ARD HQ+A LFG GATAV P+L Y QV+A + G+ + P NYRK L+KGL KI+SKMGIS L SY+G+Q+FEA+G+ EV++ FTG+ SRI G GFAE+ + + + A++ P K++ G G +Y E HA DVIK L V++G + + + E DP TI+D+L K V LEEVEPVE ++ RF +A MS+GALSPEAHE LA AMN GG+S+SGEGGED R+ G S IKQIASGRFGV+ YL NA+ L+IK+AQGAKPGEGGQLPG KVN LIARLR PGV LISPPPHHDIYSIEDLAQLI DLK+VNP A+V+VKLV+E G+GT+A G AKA AD I +SG+DGGTAASPL+S KHAG PWELG+ E HQ L +N LR+ + L+TDGGL+TG DVI AAILGAE FGT M+A+GC Y+R CHLNNC G+AT R + F+GT D V Y +AEE RE+MA LGVR + +L+GR V P LDLS ++ D +A + C +RN A + E+LA + G G F + + N DR++G +G +A+ Y GL + FRG AGQSFG + G+
Sbjct: 5 LHQPDEFRDNCGFGLIAHMEGQASHDLLKTAIESLTCMTHRGGIAADGKTGDGCGLLLKMPESFMREVAREALGVELGERFAVGSIFLPDDDAREAQGRETLEAELTKRGLNVLGWREVPVDPSVCGPMALDCLPRIRQLFVE-PGEETGDTFDVDLFMARRHAEQALRDEEDFYVCSLSPEVVSYKGLVMPVDLPAFYHDLGDTRLETAICVFHQRFSTNTAPRWPLAQPFRLLAHNGEINTIQANRGWANSRKENFTSERL-PDIAELDEIVNTTGSDSSSMDNMLEVLLTGGMDLHRAVRMMVPPAWQNVEIMDGDLRAFYEYNSMHAEPWDGPAGVVMTDGRQAVCMLDRNGLRPARWVITRNGYITLASEIGTYDYRPEDVVAKGRVGPGQILAVDTETGEVLHTEDIDSRLKSAYPYKRWLKQEASYLESALTE--LARFQNMDADTLAVQQKMFQISFEERDQILRPLAESGQEGVGSMGDDTPMAVLSTKQRLLTDYFRQKFAQVTNPAIDPLREAIVMSLESCIGAELNVFKATPEHAHRLILTTPVLSPRKFTALVTQEDPAFASQTLSLAYDPET--TGLKDALQALCAEAEKAARGDKVLLVLSDANLEKGQLPIHAALAVGAVHHHLGRLALRPRVNLIVETGYARDAHQMAVLFGVGATAVYPWLAY----QVMAD--MHRTGELVGNPADARENYRKGLQKGLFKILSKMGISTLASYRGSQLFEAVGLASEVMDMCFTGMASRIEGTGFAELQLQQELLAKDAWK----PRKSISHG--GLMKYVHGHEYHAYNPDVIKALQEAVQEGDYTKWKKFAALVNERDPATIRDLLRLKPAETPVPLEEVEPVENLLPRFDSAGMSLGALSPEAHEALAQAMNETGGRSNSGEGGEDPVRY-----GTIRSSKIKQIASGRFGVTPAYLANAEVLQIKVAQGAKPGEGGQLPGGKVNELIARLRYAVPGVTLISPPPHHDIYSIEDLAQLIFDLKQVNPDAQVSVKLVSEPGIGTIATGVAKAYADLITVSGYDGGTAASPLTSIKHAGSPWELGLPEVHQALRINGLRDKIRLQTDGGLKTGLDVIKAAILGAESFGFGTAPMVALGCKYLRICHLNNCATGVATQHQVLRDEHFRGTVDMVKHYFRFIAEEVRELMAMLGVRQLTDLIGRTDLLEAIEGVT--PSQRRLDLSPLMTNDFVPAEAPQFCQVDRNVPHDPGAKNQEVLAAMKTAIEQKSG----------GEF--EFAITNCDRSVGALASGTIAKRYGEAGLEDAPVTARFRGVAGQSFGVWNARGL 1287
BLAST of hypothetical protein vs. SwissProt
Match: gi|729601|sp|Q05755.1|GLTB_AZOBR (RecName: Full=Glutamate synthase [NADPH] large chain; AltName: Full=Glutamate synthase subunit alpha; Short=GLTS alpha chain; AltName: Full=NADPH-GOGAT; Flags: Precursor) HSP 1 Score: 1097.8 bits (2838), Expect = 0.000e+0 Identity = 600/1318 (45.52%), Postives = 803/1318 (60.93%), Query Frame = 0
Query: 21 NETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPADIGVGVFFLPEGISANVKSFTESVLTRRNIPF----LGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKEAA--FYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALV-PTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDAIRICTHE-RNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
+E CG+G IA + G+ +++ + ++ + HRGAVDAD KTGDG+G+ +P F G + VG FLP IS + + ++ + F GWR+VP+N D +G+ A T+PEI ++V V FE L+++RR +E+ E FYI +LS+R I YKG+ + L FY DL D F++ ++YHQR+STNTFP WPL QPFRM+ HNGEINTV+GN NWM + E E +G + L+ ++ G SDS SLD E++V +GR+ MLVP A + Q + +A Y S EPWDGPA L TDGR + G+DRNGLRP R+T+T DGL+ GSE+G + + ++++I +GRLGPG+M++ D Q+G+ D+E+K+ LA KP+ +WV +N L A+L D + +L R+Q G+T ED++++ PM++ +EA+ SMGDD P+A LS R L +F+Q F+QVTNPPID +RE VM+L LG N+L E R+L LES +L E + R G ++ ID T+P G L+ AL RI E E AV +IL+D A R IP++LATG+VH HL R R TSL + T E DTH A L G GAT V YL ++ +A + L + E GMANY+KA++ GLLKIMSKMGISV++SY+G FEA+G+ + +V F + SRI G+G I ++ L +H A+ +V L +G G+ R+RKSG+ H VI L V + + Y + + P+ ++D+L + A V ++EVE + I KRF T MSMGALSPEAH TL +AMNR+G KSDSGEGGED RF+P NGD S IKQ+ASGRFGV+A YL ELEIK+AQGAKPGEGGQLPG KV +IARLR++ PGV LISPPPHHDIYSIEDLAQLI+DLK++NP AKVTVKLV+ SG+GT+AAG AKA+AD ILISG+ GGT ASP +S K AG+PWE+G+SE HQVL +N LR+ V LRTDGGL+TGRD++IAA+LGAE+ GT ++IAMGC+ VR+CH N CPVG+ K R KF GTP+ VV +AEE REI+A LG RS+NE++GR Q +H + LDL+ LA + R CT + RN+ LD I+AD EG+ +Y NT R IGTR + V + GL I + RG+AGQS G F GI
Sbjct: 32 DEHDACGVGFIAAIDGKPRRSVVEKGIEALKAVWHRGAVDADGKTGDGAGIHVAVPQKFFKDHVKVIGHRAPDNKLAVGQVFLPR-ISLDAQEACRCIVETEILAFGYYIYGWRQVPINVDIIGEKANATRPEIEQIIVGNNKGVSDEQFELDLYIIRRRIEKAVKGEQINDFYICSLSARSIIYKGMFLAEQLTTFYPDLLDERFESDFAIYHQRYSTNTFPTWPLAQPFRMLAHNGEINTVKGNVNWMKAHETRMEHPAFGTHMQDLKPVIGVGLSDSGSLDTVFEVMVRAGRTAPMVKMMLVPQALTSSQTTPDNHKALIQYCNSVMEPWDGPAALAMTDGRWVVGGMDRNGLRPMRYTITTDGLIIGGSETGMVKIDETQVIEKGRLGPGEMIAVDLQSGKLYRDRELKDHLATLKPWDKWV-QNTTHLDELVKTASLKGEPSDMDKAELRRRQQAFGLTMEDMELILHPMVEDGKEAIGSMGDDSPIAVLSDKYRGLHHFFRQNFSQVTNPPIDSLRERRVMSLKTRLGNLGNILDEDETQTRLLQLESPVLTTAEFRAM--RDYMGDTAAEIDATFPVDGGPEALRDALRRIRQETEDAVRGGATHVILTDEAMGPARAAIPAILATGAVHTHLIRSNLRTFTSLNVRTAEGLDTHYFAVLIGVGATTVNAYLA----QEAIAERHRRGLFGSMPLEKGMANYKKAIDDGLLKIMSKMGISVISSYRGGGNFEAIGLSRALVAEHFPAMVSRISGIGLNGIQKKVLEQHATAYNEEV---VALPVG--GFYRFRKSGDRHGWEGGVIHTLQQAVTNDSYTTFKKYSEQVNKRPPMQLRDLLELRSTKAPVPVDEVESITAIRKRFITPGMSMGALSPEAHGTLNVAMNRIGAKSDSGEGGEDPARFRPDKNGDNWNSAIKQVASGRFGVTAEYLNQCRELEIKVAQGAKPGEGGQLPGFKVTEMIARLRHSTPGVMLISPPPHHDIYSIEDLAQLIYDLKQINPDAKVTVKLVSRSGIGTIAAGVAKANADIILISGNSGGTGASPQTSIKFAGLPWEMGLSEVHQVLTLNRLRHRVRLRTDGGLKTGRDIVIAAMLGAEEFGIGTASLIAMGCIMVRQCHSNTCPVGVCVQDDKLRQKFVGTPEKVVNLFTFLAEEVREILAGLGFRSLNEVIGRTDLLHQVSRGAEH--LDDLDLNPRLAQVDPGENARYCTLQGRNE--VPDTLDARIVAD--ARPLFEEGEK----------MQLAYNARNTQRAIGTRLSSMVTRKFGMFGLQPGHITIRLRGTAGQSLGAFAVQGI 1320
BLAST of hypothetical protein vs. SwissProt
Match: gi|300680980|sp|Q69RJ0.2|GLTB_ORYSJ (RecName: Full=Ferredoxin-dependent glutamate synthase, chloroplastic; AltName: Full=Fd-GOGAT; Flags: Precursor) HSP 1 Score: 1093.18 bits (2826), Expect = 0.000e+0 Identity = 606/1353 (44.79%), Postives = 827/1353 (61.12%), Query Frame = 0
Query: 16 LHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTG-DPADIGVGVFFLP--EGISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKEAAF----YIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEIS---EEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELK--SFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEG-EAGLKKALDRICAEAETAVNDKIEILILSDRATSAD--RVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDV------KGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYP-----------------NGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
L+E +E CG+G +ANL+ E S++I+ AL ++ M HRG AD +GDGSG++ IP LF A G D + GVG+ FLP E K+ V T + LGWR VP N +G A+ T P I + V E +A ER L++ R+ +ER TK A++ Y +LSSR I YKG+ L FY DLQ+ +K+ ++YH+R+STNT P WPL QP R++ HNGEINT++GN NWM SRE +S VW H +R SDSA+LD ELL+ SGRS AM +LVP A++N +S EV FY+Y + E WDGPA L F+DGR + A LDRNGLRP+R+ T D +Y+ SE G I + +SK++ +GRLGPG M++ D QTG+ L + E+K+ +A+ PY W+ ++ +K +F S A+ + + R Q G ++ED+ MV M +E F MGDDIPLA LS+ P +LF YFKQ FAQVTNP IDP+RE VM+L +G +N+L PE+A + L S +L E ELE++ N + V+ T + +G + L KA+ +C EA+ AV + ++L+LSDR+ + + R IP LLA G++H HL + R+ S+V DT + THQ ACL G+GA+A+CPYL T RQ S+ GK+ +T E N+ KA++ GLLKI+SKMGIS+L+SY GAQIFE G+G+EVV+ AF G S+IGG+ E+ E+L AF D K L+ + G+ + R GE HA ++ K LH VR+ Y Y + ++D+L K A + + +VEP IV+RF T MS+GA+S E HE +AIAMNR+GGKS+SGEGGED R+ P NGD A S IKQ+ASGRFGV+ +LVNA+++EIK+AQGAKPGEGGQLPG KV+A IARLRN++PGV LISPPPHHDIYSIEDLAQLI+DL ++NP+AKV+VKLVAE+G+GTVA+G +K +AD I ISGHDGGT ASP+SS KHAG PWELG+SE HQ L+ N LR V LR DGG R+G DV++AA +GA++ FG++AMIA GCV R CH NNCPVG+A+ + RA+F G P +V Y + VAEE R +A LG +++++GR L+ + V K +DL +L+ ++ + P LD ILAD + + + + +K++ + N DR + R AG +A+ Y + G A +++TF GSAGQSFG FL G+
Sbjct: 87 LNEILSERGACGVGFVANLKNEPSFNIVRDALVALGCMEHRGGCGADNDSGDGSGLMSGIPWDLFNDWANKQGLAPLDRTNTGVGMVFLPQDENSMEEAKAVVAKVFTDEGLEVLGWRTVPFNVSVVGRYAKETMPNIQQIFVKVAKEDNADDIERELYICRKLIERA-TKSASWADELYFCSLSSRTIVYKGMLRSEILGQFYLDLQNELYKSPFAIYHRRYSTNTSPRWPLAQPMRLLGHNGEINTIQGNLNWMRSREATLQSPVWRGREHEIRPFGDPKASDSANLDSTAELLLRSGRSPAEAMMILVPEAYKNHPTLSIKYPEVIDFYDYYKGQMEAWDGPALLLFSDGRTVGACLDRNGLRPARYWRTSDDFVYVASEVGVIPMDESKVVMKGRLGPGMMITVDLQTGQVLENTEVKKSVASANPYGSWLQQSTRSIKPVNFQSSVAM------DNETVLRHQQAFGYSSEDVQMVIETMASQGKEPTFCMGDDIPLAVLSQKPHMLFDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRRNILEVGPENADQVTLSSPVLNEGELESLLN--DSKLKPKVLSTYFDIRKGLDGSLDKAIKVLCDEADAAVRNGSQLLVLSDRSEALEPTRPAIPILLAVGAIHQHLIQNGLRMSASIVADTAQCFSTHQFACLIGYGASAICPYLALETCRQWRLSNKTVNLMRNGKM-PTVTIEQAQRNFIKAVKSGLLKILSKMGISLLSSYCGAQIFEIYGLGQEVVDLAFCGSVSKIGGLTLDELGRETLSFWVKAFSEDT--AKRLE--NFGFIQSRPGGEYHANNPEMSKLLHKAVREKSDNAYTVYQQHLASRPVNVLRDLLELKSDRAPIPIGKVEPATSIVERFCTGGMSLGAISRETHEAIAIAMNRIGGKSNSGEGGEDPIRWSPLADVEDGYSPTLPHLKGLQNGDTATSAIKQVASGRFGVTPTFLVNAEQIEIKIAQGAKPGEGGQLPGKKVSAYIARLRNSKPGVPLISPPPHHDIYSIEDLAQLIYDLHQINPKAKVSVKLVAEAGIGTVASGVSKGNADIIQISGHDGGTGASPISSIKHAGGPWELGLSETHQTLIQNGLRERVVLRVDGGFRSGLDVLMAAAMGADEYGFGSVAMIATGCVMARICHTNNCPVGVASQREELRARFPGVPGDLVNYFLFVAEEVRATLAQLGFEKLDDIIGR-TDILKAKHV-SLAKTQHIDLKYLLSSAGLPKWSSSQIRSQDVHSNGPVLDETILAD----------PDISDAIENEKEVSKTFQIYNVDRAVCGRVAGVIAKKYGDTGF-AGQLNITFTGSAGQSFGCFLTPGM 1412
BLAST of hypothetical protein vs. SwissProt
Match: gi|121446|sp|P23225.1|GLTB_MAIZE (RecName: Full=Ferredoxin-dependent glutamate synthase, chloroplastic; AltName: Full=Fd-GOGAT; Flags: Precursor) HSP 1 Score: 1080.86 bits (2794), Expect = 0.000e+0 Identity = 601/1360 (44.19%), Postives = 823/1360 (60.51%), Query Frame = 0
Query: 6 PDHTPDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFT-GDPADIGVGVFFLP--EGISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVER---EFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEIS---EEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEG-EAGLKKALDRICAEAETAVNDKIEILILSDRATSAD--RVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDV------KGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYP-----------------NGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
P + L+ +E CG+G +ANL+ S+DI+ AL ++ M HRG AD +GDG+G++ +P LF A+ G D + GVG+ FLP E K+ TE V + LGWR VP N +G A+ T P I + V E +A ER L++ R+ +ER F+ Y +LSSR I YKG+ L FY DLQ+ +K+ ++YH+RFSTNT P WPL QP R++ HNGEINT++GN NWM SRE +S VW H + SDSA+LD ELL+ SGRS A+ +LVP A++N +S EV FY+Y + E WDGPA L F+DGR + A LDRNGLRP+R+ T D +Y+ SE G I + +SK++ +GRLGPG M++ D QTG+ L + E+K+ +A+ PY W+ E +K ++ + + + R Q G ++ED+ MV M +E F MGDDIPLA LS+ P LL+ YFKQ FAQVTNP IDP+RE VM+L +G N+L PE+A +AL S +L E ELET+ N + V+ T + +G + L K + +C EA+ AV ++L+LSDR+ + + R IP LLA G++H HL + R+ S+V DT + TH ACL G+GA+AVCPYL T RQ S+ GK+ +T E N+ KA++ GLLKI+SKMGIS+L+SY GAQIFE G+G+EVV+ AF G S+IGG+ E+ E+L AF D K L+ + G+ + R GE HA ++ K LH +R+ + Y Y + ++D+L K A + + +VE IV+RF T MS+GA+S E HE +AIAMNR+GGKS+SGEGGED R+ P NGD A S IKQ+ASGRFGV+ +LVNAD++EIK+AQGAKPGEGGQLPG KV+A IARLRN++PGV LISPPPHHDIYSIEDLAQLI+DL ++NP+AKV+VKLV+E+G+GTVA+G +KA+AD I ISGHDGGT ASP+SS KHAG PWELG++E +Q L+ N LR V LR DGG R+G+DV+IAA +GA++ FG++AMIA GCV R CH NNCPVG+A+ + RA+F G P +V Y + VAEE R +A LG +++++GR L+ + + K +DL +L++ + ++ + P LD ILAD E A E + +K++ + N DR + R AG +A+ Y + G A +++TF GSAGQSFG FL G+
Sbjct: 78 PQKPTQQAADLNHILSERGACGVGFVANLKNMSSFDIVRDALMALGCMEHRGGCGADSDSGDGAGLMSAVPWDLFDDWASKQGLALFDRRNTGVGMVFLPQDEKSMEEAKAATEKVFVDEGLEVLGWRPVPFNVSVVGRNAKETMPNIQQIFVKVAKEDNADDIERELYISRKLIERAAKSFSWADELYFCSLSSRTIVYKGMLRSEVLGQFYLDLQNELYKSPFAIYHRRFSTNTSPRWPLAQPMRLLGHNGEINTIQGNLNWMRSRETTLKSPVWRGREHEICPFGDPKASDSANLDSTAELLLRSGRSPAEALMILVPEAYKNHPTLSIKYPEVTDFYDYYKGQMEAWDGPALLLFSDGRTVGATLDRNGLRPARYWRTSDDFVYVASEVGVIPMDESKVVMKGRLGPGMMITVDLQTGQVLENTEVKKTVASASPYGTWLQECTRLIKPVNFLSSTIMDNE----TVLRHQQAFGYSSEDVQMVIESMASQGKEPTFCMGDDIPLAVLSQRPHLLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILEVGPENADQVALSSPVLNEGELETLLN--DSKLKPKVLSTYFDIRKGLDGSLDKTIQALCEEADAAVRSGSQLLVLSDRSEAPEPTRPAIPILLAVGAIHQHLIQNGLRMSASIVADTAQCFSTHHFACLIGYGASAVCPYLALETCRQWRLSNKTLNLMRNGKM-PTVTIEQAQRNFIKAVKSGLLKILSKMGISLLSSYCGAQIFEIYGLGQEVVDLAFCGSVSKIGGLTLDELGRETLSFWVKAFSEDT--AKRLE--NFGFIQSRPGGEYHANNPEMSKLLHKAIREKRDNAYTVYQQHLASRPVNVLRDLLELKSDRAPIPIGKVESATSIVERFCTGGMSLGAISRETHEAIAIAMNRIGGKSNSGEGGEDPIRWNPLTDVVDGYSPTLPHLKGLQNGDTATSAIKQVASGRFGVTPTFLVNADQIEIKIAQGAKPGEGGQLPGKKVSAYIARLRNSKPGVPLISPPPHHDIYSIEDLAQLIYDLHQINPKAKVSVKLVSEAGIGTVASGVSKANADIIQISGHDGGTGASPISSIKHAGGPWELGLTETNQTLIQNGLRERVVLRVDGGFRSGQDVLIAAAMGADEYGFGSVAMIATGCVMARICHTNNCPVGVASQREELRARFPGVPGDLVNYFLFVAEEVRAALAQLGYEKLDDIIGR-TDLLKPKHI-SLVKTQHIDLGYLLSNAGLPEWSSSQIRSQDVHTNGPVLDETILAD-PEIADAIENEKEV---------SKAFQIYNVDRAVCGRVAGVIAKKYGDTGF-AGQLNITFNGSAGQSFGCFLTPGM 1413
BLAST of hypothetical protein vs. SwissProt
Match: gi|12644435|sp|Q43155.3|GLTB_SPIOL (RecName: Full=Ferredoxin-dependent glutamate synthase, chloroplastic; AltName: Full=Fd-GOGAT; Short=SoFdGOGAT) HSP 1 Score: 1075.85 bits (2781), Expect = 0.000e+0 Identity = 602/1343 (44.83%), Postives = 825/1343 (61.43%), Query Frame = 0
Query: 26 CGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTG-DPADIGVGVFFLPEG--ISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVER---EFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEIS---EEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKS--FTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEG-EAGLKKALDRICAEAETAVNDKIEILILSDRATSAD--RVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVK------GKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITI-KDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYP-----------------NGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
CG+G IANL + S+ I+ ALT++ M HRG +D +GDGSGV+ IP LF + G D + GVG+ FLP+ ++ K + I +GWR VP N +G A+ T P I + V E ER L++ R+ +ER T + Y +LS++ I YKG+ L FY DLQ+ + + ++YH+R+STNT P WPL QP R + HNGEINT++GN NWM SRE +S VW + +R SDSA+LD A ELL+ SGR+ E A+ +LVP A++N + E FY+Y + E WDGPA L F+DG+ + A LDRNGL P+R+ T D ++Y+ SE G + + +SK+ +GRLGPG M+S D +G+ + E+K+R+A+ PY +WV EN LK+ F S A L E+ L R Q G ++ED+ MV M +E F MGDDIPLA +S+ P +L+ YFKQ FAQVTNP IDP+RE VM+L +G N+L PE+A + L S +L E ELE + N + + ++ + +G E L+K L+R+C A+ AV + ++L+LSDR+ + R IP LLA G+VH HL + R+ TS+V+DT + THQ ACL G+GA+A+CPYL T RQ S+ GK+ +T E N+ KA++ GLLKI+SKMGIS+L+SY GAQIFE G+GK+VV+ AF G S++GG+ E+A E+L AF D K L+ + G+ ++R GE H ++ K LH VR+ + Y Y + + N P+++ +D+L FK A +++ +VEP IV+RF T MS+GA+S E HE +AIAMNRLGGKS+SGEGGED R++P NGD A S IKQ+ASGRFGV+ +LVNAD++EIK+AQGAKPGEGGQLPG KV+A IARLRN++PGV LISPPPHHDIYSIEDLAQLI+DL ++NP+AKV+VKLVAE+G+GTVA+G AK +AD I +SGHDGGT ASP+SS KHAG PWELG+SE HQ L+ N LR V LR DGGL+ G DV++AA +GA++ FG++AMIA GCV R CH NNCPVG+A+ + RA+F G P +V + + VAEE R I+A LG +++++GR L+ R++ K LDLS +LA ++ + P LD +IL+D E A E + K+ + N DR + R AG +A+ Y + G A ++LTF GSAGQSF FL G+
Sbjct: 1 CGVGFIANLDNKGSFQIVKDALTALGCMEHRGGCGSDNDSGDGSGVMTAIPWDLFNDWGKDQGIGPFDRSHTGVGMVFLPKDDLLAEEAKKVVLDTFAQEGIEVIGWRSVPTNVSVVGRNAKETMPNIQQVFVRIIKEDSTDDIERELYICRKLIERAASSHTWASELYFCSLSNQTIIYKGMLRSEVLGMFYYDLQNERYTSPFAIYHRRYSTNTSPRWPLAQPMRFLGHNGEINTIQGNLNWMRSREPSIQSPVWRGRENEIRPYGNPKASDSANLDSAAELLIRSGRTPEEALMILVPEAYKNHPTLMIKYPEAVDFYDYYKGQMETWDGPALLLFSDGKTVGACLDRNGLAPARYWRTVDNVVYVASEVGVLPMDESKVTMKGRLGPGMMISVDLSSGQVYENTEVKKRVASSNPYGKWVKENLRSLKAVNFLSRALL---ENDTIL---RNQQAFGYSSEDVQMVIESMASQGKEPTFCMGDDIPLAVMSQKPHMLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILEVGPENASQVILPSPVLNEGELEALVN--DPLLKAQMLPIFFDIRKGVEGTLEKRLNRLCEAADEAVRNGSQMLVLSDRSEELEPTRPAIPILLAVGAVHQHLIQNGLRMYTSIVVDTAQCFSTHQFACLIGYGASAICPYLALETCRQWRLSNKTVNLMRTGKI-PTVTIEQAQNNFCKAVKSGLLKILSKMGISLLSSYCGAQIFEIYGLGKDVVDIAFQGSVSKMGGLTLDELARETLSFWVKAFSEDT--AKRLE--NFGFIQFRPGGEYHVNNPEMSKLLHKAVRNKSESAYAVYQQ-HLANRPVSVLRDLLEFKSDRAPISVGKVEPATSIVERFCTGGMSLGAISRETHEAIAIAMNRLGGKSNSGEGGEDPIRWRPLTDVVDGYSSTLPHLKGLQNGDTATSAIKQVASGRFGVTPTFLVNADQIEIKIAQGAKPGEGGQLPGKKVSAYIARLRNSKPGVPLISPPPHHDIYSIEDLAQLIYDLHQINPKAKVSVKLVAEAGIGTVASGVAKGNADIIQVSGHDGGTGASPISSIKHAGGPWELGLSETHQTLISNGLRERVILRVDGGLKCGVDVMMAAAMGADEYGFGSLAMIATGCVMARICHTNNCPVGVASQREELRARFPGVPGDLVNFFLYVAEEVRGILAQLGFEKLDDIIGR-TDILKPRDI-SLMKTQHLDLSYILASAGLPTMSSTAIRKQEVHTNGPVLDDQILSD-PEIIDAIENEKIV---------NKTVKIFNVDRAVCGRIAGVIAKKYGDTGF-AGQLNLTFEGSAGQSFAVFLTPGM 1316
BLAST of hypothetical protein vs. nr
Match: gi|1200392942|gb|OUV11015.1| (glutamate synthase large subunit, partial [Verrucomicrobiaceae bacterium TMED86]) HSP 1 Score: 2311.18 bits (5988), Expect = 0.000e+0 Identity = 1097/1277 (85.90%), Postives = 1193/1277 (93.42%), Query Frame = 0
Query: 58 AVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPADIGVGVFFLPEGISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGILSTV 1334
AVDAD KTGDGSGVLCQIP +FAKAAA FG++G D+ VGV+FLPE +A +KSFTESVLTRRNIP +GWREVPVNPDELG LA TQP I+HLLVSRPT+VDA HFERLLFLVRREVEREF ++A FYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDF+TGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESE+WGDD+ FL++L+ +GESDSASLDHALELLVLSGR LEHAMCMLVPPAFR+DQEISE VRAFYNYIRSF+EPWDGPAGL FTDG +CAGLDRNGLRPSR+TLT++GLLYIGSESGA++LP ++IRRGRLGPGQM+SA+T++GEF D+EIKE+LAAQKPY +W+DE+R+ELK FTSH A +P+ DF ++LSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVM+LGAGLG EQNLLTET +HAR+L L SAILFEQEL+T+ NR EHGFS+LVIDTTWP SEGEAGLK ALDRICAEAETAV++ EILI+SDRATSA+RVPIPSLLATGS+HHHLNRCRKRLR S+V+DTGEARDTHQIACLFGFGATAVCPYLG++TVRQVVA+D KGKLG ++TPE M+NYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVV++AFTG HSRIGGVGFAEIAEESLVRHRAAF+VD E GKTL+LGDPGYNRYRKSGE+HALTTDVIKN HTFVRDGKKEDYDDYVKVT+ +DPITIKDIL FKVPA+GV+L+EVEP EEIVKRFTTAAMSMGALSPEAHETLAIAMNR+GGKSDSGEGGED RRF+PY NGD ARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNP+AKVTVKLV+ESGVGTVAAG AKASADNILISGHDGGTAASPLSSTKHAG+PWELGISEAHQVLMMNNLRN VTLRTDGGLRTGRDV+IAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVG+ATT PKWRAKFKGTPDHVV +MMGVAEEAREIMA+LGVRS+N+L+GRPQ++L+QREVPDHPKANT+DLSAVLADKA DDA+RICT ERNDG HKPALDL+IL DLA HT +E NPTAG +DR PF K Y VVNTDRNIGTRTAGRVAEIY NH LPAN+I+LTF+GSAGQSFGTFLCGGI T+
Sbjct: 1 AVDADMKTGDGSGVLCQIPRPIFAKAAAGFGYSGSADDLAVGVYFLPEDGAAQIKSFTESVLTRRNIPLIGWREVPVNPDELGKLAFDTQPLITHLLVSRPTDVDADHFERLLFLVRREVEREFKEQANFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFQTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEIWGDDVEFLKDLLSDGESDSASLDHALELLVLSGRRLEHAMCMLVPPAFRHDQEISESVRAFYNYIRSFAEPWDGPAGLVFTDGTTLCAGLDRNGLRPSRYTLTDNGLLYIGSESGAVVLPSERVIRRGRLGPGQMISANTESGEFFNDREIKEQLAAQKPYAKWIDEHRIELKDFTSHEAHIPSVDFEPVELSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMSLGAGLGPEQNLLTETAKHARILQLGSAILFEQELDTVINRTEHGFSTLVIDTTWPTSEGEAGLKTALDRICAEAETAVDNGTEILIISDRATSAERVPIPSLLATGSIHHHLNRCRKRLRCSVVVDTGEARDTHQIACLFGFGATAVCPYLGFATVRQVVATDTKGKLG-EVTPEKAMSNYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVDYAFTGAHSRIGGVGFAEIAEESLVRHRAAFEVDPEEGKTLNLGDPGYNRYRKSGESHALTTDVIKNFHTFVRDGKKEDYDDYVKVTLASDPITIKDILDFKVPASGVSLDEVEPAEEIVKRFTTAAMSMGALSPEAHETLAIAMNRIGGKSDSGEGGEDPRRFQPYANGDHARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPKAKVTVKLVSESGVGTVAAGVAKASADNILISGHDGGTAASPLSSTKHAGLPWELGISEAHQVLMMNNLRNLVTLRTDGGLRTGRDVVIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGVATTDPKWRAKFKGTPDHVVNFMMGVAEEAREIMATLGVRSLNDLIGRPQQYLEQREVPDHPKANTIDLSAVLADKADDDAVRICTSERNDGIHKPALDLQILQDLAAHTGGSETGNPTAGCLDRNPFEKEYEVVNTDRNIGTRTAGRVAEIYGNHDLPANTINLTFKGSAGQSFGTFLCGGIKLTL 1276
BLAST of hypothetical protein vs. nr
Match: gi|1200263779|gb|OUT86727.1| (glutamate synthase large subunit [Roseibacillus sp. TMED18]) HSP 1 Score: 2023.82 bits (5242), Expect = 0.000e+0 Identity = 978/1361 (71.86%), Postives = 1130/1361 (83.03%), Query Frame = 0
Query: 1 MHLYHPDHTPDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFT-GDPADIGVGVFFLP---EGISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPA--AGVALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDA----------IRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNP----------TAG-----LMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
M+ YHP TP+++ +LHEWSNE+ NCGMGAIA L GE++Y +D+A+TSVCNMTHRGAVDAD KTGDG+GVL QIP+ +F K A+ G D+ VGVFFLP + + V+S TES++ RR I +GWR+VPV+PDELG++AR TQPEI HL++ +P D+ +ER LFLVRRE+ER+F FYIPTLSSRLISYKGLA P TLRAFY+DLQDSD++ GISLYHQRFSTNTFPAWPLGQPFRM+CHNGEINTVRGNRNWMASREEFF S++WGDDI L+NL+ ESDSASLDHA ELLVLSGRSLEHAMCMLVPPAFR+D EIS++VR+FY+Y+ SF EPWDGPAGL +TDG K+CA LDRNGLRPSRFTLT DGLLYIGSESGA+ +PD K+IRRGRLGPGQMLSADT +G+ +D+EIKE LA Q+PY +W+DENR+ELK F + VP +D + L LSR QVTHG+T EDLDMVFPPMIK AQEAV SMGDDIPLAPLSR+PRLLFTYFKQLFAQVTNPPIDPIREWAVMTL AGLG E+NLL ETPEH R+L LESAILFEQELE + N++EHGF ++ ID TW EG GLK ALDR+C AE AV+D + ILILSDRA S RV +PSLL TG+VHHHLN RKR+R S+V++TGEARDTHQIACLFGFGATAVCPYLGY+TVRQVVA+D + KLG+ +TP + MANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEA+G+G+ V+++ F G HSRI GVGF++IAEES++RHRAA++ D+ G+TLDLGDPGYNRYR++GE HALTT+VIKN HTFV+ G K+ Y+DYVK T+E P TIKD+L F VP VA+EEVEP+E I +RFTTAAMSMGALSPEAHETLAIAMN +GGKSDSGEGGED RRF+PYPNGD+ARS IKQ+ASGRFGVSAHYLVN DE+EIKMAQGAKPGEGGQLPGHKVN LIARLR+TQPGVQLISPPPHHDIYSIEDLAQLIHDLKE+NPRAKVTVKLVAESGVGT+AAG AKA+AD IL+SGHDGGT ASPLSS KHAG+PWELG+SE Q L++NNLR VTLRTDGG+RTGRD++IAA+LGAE+ NFGTIAMIAMGCVYVRKCHLN CPVGIATTSPK+RAKFKGTP+ V+ + VA EAREIMAS+GVR +++L+G P +L+QREV DH KANT++LS VL D A + A RI ERNDG HKPALDL I+ADLA T A G+N AG LMD+ P + +Y VVNTDRNIGTR AGRVAEIY N GLP +I+LTFRGSAGQSFG FLC GI
Sbjct: 1 MNPYHPAFTPNTTDSLHEWSNESDNCGMGAIALLNGERNYQTLDHAITSVCNMTHRGAVDADMKTGDGAGVLTQIPYPIFKKVASALGHELRSDKDLAVGVFFLPRDNKEKQSEVQSATESIVARRGITLIGWRQVPVHPDELGEVARETQPEILHLILEKPATWDSDFYERQLFLVRRELERQFGDLTGFYIPTLSSRLISYKGLAQPETLRAFYADLQDSDYQIGISLYHQRFSTNTFPAWPLGQPFRMLCHNGEINTVRGNRNWMASREEFFASDLWGDDIELLKNLLSFDESDSASLDHAFELLVLSGRSLEHAMCMLVPPAFRHDPEISDQVRSFYSYLSSFVEPWDGPAGLVYTDGTKVCASLDRNGLRPSRFTLTSDGLLYIGSESGAVQIPDGKVIRRGRLGPGQMLSADTTSGKLKFDREIKEGLATQQPYGKWIDENRIELKDFVTPDPQVPADDLDPLYLSRCQVTHGITLEDLDMVFPPMIKAAQEAVHSMGDDIPLAPLSRHPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLAAGLGPERNLLEETPEHGRILQLESAILFEQELEALRNKEEHGFLTISIDATWDLEEGPGGLKTALDRVCQLAEKAVDDGVGILILSDRAFSETRVALPSLLVTGAVHHHLNGVRKRMRASVVVETGEARDTHQIACLFGFGATAVCPYLGYATVRQVVATDNRKKLGETMTPHLAMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEAIGIGQSVIDYCFAGTHSRIAGVGFSDIAEESIIRHRAAWETDIPEGETLDLGDPGYNRYRRTGERHALTTEVIKNFHTFVKTGDKDTYEDYVKATLETTPSTIKDLLEF-VPGLQGAVAIEEVEPIEAIRRRFTTAAMSMGALSPEAHETLAIAMNAIGGKSDSGEGGEDPRRFEPYPNGDWARSRIKQVASGRFGVSAHYLVNCDEIEIKMAQGAKPGEGGQLPGHKVNGLIARLRHTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEINPRAKVTVKLVAESGVGTIAAGVAKANADIILVSGHDGGTGASPLSSIKHAGLPWELGVSETQQTLVLNNLRGKVTLRTDGGMRTGRDIVIAALLGAEEFNFGTIAMIAMGCVYVRKCHLNTCPVGIATTSPKFRAKFKGTPEMVIRFFDSVAHEAREIMASIGVRRMDDLIGHP-GYLRQREVTDHNKANTINLSTVLKDVAPEAAKTSGVPLERIARIHQIERNDGIHKPALDLRIIADLANKT-APRGENADTSTKPDSAQCAGDTFLKLMDQPPISLAYDVVNTDRNIGTRLAGRVAEIYGNSGLPDGTINLTFRGSAGQSFGCFLCSGI 1358
BLAST of hypothetical protein vs. nr
Match: gi|1110140585|emb|SHJ72061.1| (glutamate synthase (NADPH/NADH) large chain [Rubritalea squalenifaciens DSM 18772]) HSP 1 Score: 1955.26 bits (5064), Expect = 0.000e+0 Identity = 947/1342 (70.57%), Postives = 1091/1342 (81.30%), Query Frame = 0
Query: 4 YHPDHTPDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPA-DIGVGVFFLPEG---ISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAG-VALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAG----------DDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
Y +TPD++G+LH +SNE NCGMGAI + G+ S I+D A+ SVCNMTHRGAVDAD KTGDGSG+L QIP LFA+ AA G + A DI VGVFF+P G +KS E ++ R I +GWREVPV P+ELG+LA+ TQP I HLL+++P+ D+ FER L+L RR VE FY+P+ S RLISYKGLAMPATLRAFY D+QD DF+T I+LYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFES+VWGDD++ ++NL+ + ESDSASLDHALELL LSGRS+EH+MCMLVPPAFRND +IS+E+R FY Y RSF+EPWDGPAGL +TDG K+CA LDRNGLRPSR+ LTEDGLLYIGSE GA+ L S IIR+GRLGPGQML AD TGE D+E+KE LA Q PY RWVDEN++ L+ + S VP DF+ L+R Q+ +G++ EDLDMVFPPMIKGAQEAVFSMGDDIPLA LS YPRLL+TYFKQLFAQVTNPPIDPIREWAVM+L AGLGAE+N+L ETPEHA VL LESAIL EQE+E I EHG+ S +DTTW SEG G+ KA+ R+C EAE A+ + I ILILSDRA++ +RV IP+LL TG+VHHHLNR RKRLR SLVI+T EARDTHQIACLFGFGATAVCPYLGY+TVRQV+A+D KGKL + +TPE+GMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEA+G+G +VV+ FT SRIGG+GFAEIAEES++RH+A D+ G+ L LGDPGYNRYRK GE HALTTDVIKN HTFV+ GK EDY+DYVK ++E P+TIKD+ F ++G V +EEVEP+E+I +RFTTAAMS+GALSPEAHETLAIAMNR+GG+SDSGEGGED +RF PYPNGD A+S IKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVN +IARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKE+NP+A+VTVKLVAE+GVGTVAAG AKASAD ILISGHDGGT ASPLSSTKH G+PWELG+SEA Q L++N LRN VTLRTDGGLRTG+D+I AAILGAE+ NFGTIAMIAMGCVYVRKCHLNNCPVG+ATT PKWRAKFKGTP+ V+ + V++EAREIMASLGVR ++EL+G P FL+QR VP+HPKAN +DL VL D D RICT ERNDG KPALDL+IL DL + A + + LMDR P Y VVNTDRNIGTR +GR+AE+Y N GLP +I L GSAGQSFGTFL GGI
Sbjct: 5 YDSQYTPDTTGSLHSFSNERDNCGMGAIVQIHGKASKKILDLAVESVCNMTHRGAVDADMKTGDGSGILSQIPRKLFAREAAALGNSSVAAEDIAVGVFFMPRGNADAETQIKSLAEKTVSDRGIAIIGWREVPVVPEELGELAQKTQPHILHLLMAKPSGWDSLQFERQLYLCRRAVEHGTKDIDNFYMPSFSGRLISYKGLAMPATLRAFYKDMQDDDFETAIALYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESDVWGDDVNLVKNLLSDNESDSASLDHALELLTLSGRSIEHSMCMLVPPAFRNDPDISDELRGFYAYHRSFAEPWDGPAGLVYTDGTKVCASLDRNGLRPSRYALTEDGLLYIGSEIGAVELDQSTIIRKGRLGPGQMLLADLSTGELKNDREVKEALAKQAPYARWVDENQLNLRDYISPDPQVPAVDFDETTLTRLQIMNGISEEDLDMVFPPMIKGAQEAVFSMGDDIPLAVLSTYPRLLYTYFKQLFAQVTNPPIDPIREWAVMSLAAGLGAERNMLDETPEHAAVLELESAILLEQEMEKIKQMGEHGYPSRQLDTTWNLSEGADGMLKAIQRVCKEAEEAIEEDISILILSDRASAPERVAIPTLLITGAVHHHLNRARKRLRASLVIETAEARDTHQIACLFGFGATAVCPYLGYATVRQVIANDTKGKL-EGVTPEIGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEAVGIGSDVVDTCFTATKSRIGGIGFAEIAEESIIRHKAGHS-DLTEGEPLTLGDPGYNRYRKQGERHALTTDVIKNFHTFVKTGKAEDYEDYVKASLETTPVTIKDLFDFVPLSSGPVPIEEVEPIEDIRRRFTTAAMSIGALSPEAHETLAIAMNRIGGRSDSGEGGEDPKRFTPYPNGDLAKSRIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNGIIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEINPKARVTVKLVAENGVGTVAAGVAKASADVILISGHDGGTGASPLSSTKHCGLPWELGLSEAQQTLLLNGLRNKVTLRTDGGLRTGKDIITAAILGAEEYNFGTIAMIAMGCVYVRKCHLNNCPVGVATTDPKWRAKFKGTPEQVINFFNAVSQEAREIMASLGVRKLDELIGHP-GFLKQRHVPEHPKANMIDLGPVLKDVTPELSKISGTPVDQISRICTQERNDGLDKPALDLQILEDLKKELAT----DDFSALMDRAPICLDYKVVNTDRNIGTRLSGRIAEVYGNTGLPCGTITLNISGSAGQSFGTFLAGGI 1339
BLAST of hypothetical protein vs. nr
Match: gi|918703691|ref|WP_052572765.1| (glutamate synthase large subunit [Haloferula sp. BvORR071]) HSP 1 Score: 1944.47 bits (5036), Expect = 0.000e+0 Identity = 946/1361 (69.51%), Postives = 1106/1361 (81.26%), Query Frame = 0
Query: 4 YHPDHTPDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFT-GDPADIGVGVFFLPEGISA---NVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGD-DIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDV-KGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAG-VALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDA----------IRICTHERNDGFHKPALDLEILADLAEHTAAAE--------GQNP-----TAGLM---DRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANS-IDLTFRGSAGQSFGTFLCGGI 1330
YHP TP G+LH S E NCGMGAIA + G++S+++ID ALTSVCNMTHRGAVDAD KTGDGSG+L QIP+ +F KAAA G T + +D+ V VFFLP +A +KS E V+ +R I +GWREVPV+PD LG +A +++P I HLL+ +P D HFER LFL RRE+ER+ FY+PT SSRLISYKGLAMPA LRAFY DLQDSDF+T ISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTV GNRNWMASREEFF +WGD DI L +LM E ESDSASLDHALE LVLSGRSLEHAMCMLVPPA+RND++IS+++RAFY +IRSFSEPWDGPAGL +TDG K+CA LDRNGLRPSR+ LTEDGLLYIGSE+GA+I+ D+K+IR+GRLGPGQM S DT TG+ D+EIKE LA KPY +W+DENR+EL+ F S AL P EDFN L LSRQQVTHG++ E+LDMVFPPMIKGAQEAVFSMGDDIPLA LS YPRLLFTYFKQ FAQVTNPPIDPIREWAVM+ AGLG E+NLL ET EH +++ LESA+LFE +++ + + + GF + V+D TWP S G AGLK+ LD +C E E AV+ + I+ILSDR +A+RVPIP++LATG+VHHHLNR RKR+R SL++++GE RDTHQI+C FGFGATAVCPYLG++TVRQ+VA+D KGKL D I+ E MANY KALEKG+LKIMSKMGISVLNSYQGAQIFEA+G+GK+VV+FAFTGV S++GG+ FAEIAEESL+RH+AAF+ +V G+TLDLGDPGYNRYRKSGE HA TTDVIKN HTFV+ GK+EDYDDYVKV++E P+ IKD+ F A+G + +EEVE VE I +RFTTAAMS+GALSPEAHETLAIAMNR+GGKSDSGEGGED+ RFKPYPNGDFARS+IKQ+ASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPG KVNALIARLR+TQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRA+VTVKLVAE+GVGTVAAG AKASAD ILISGHDGGT ASPLSSTKHAG PWELG++EA Q L++NNLR+ V +RTDGGLR G+D++IAA LGAE+ NFGTIAMIAMGCVYVRKCHLNNCPVGIATT PK+RAKFKGTP+ V+ + GVA EAR IMA LGVR+++EL+GRP+ +L+QR VP+HPKAN +DL+ +L D + A RIC ERNDG K LD+ +L DLA+ E G N TA +M DR P SY VVNTDRNIGTR +GR+AEI+ + G ++ + L RGSAGQS GTFL G+
Sbjct: 5 YHPS-TPLVPGSLHRLSAERDNCGMGAIAQIHGKRSFEVIDMALTSVCNMTHRGAVDADMKTGDGSGILSQIPYPIFRKAAAKLGTTLENDSDLAVAVFFLPLNDAAGQKQLKSLAEEVVAKRGITVIGWREVPVDPDALGKIALVSRPHIEHLLLLKPAAWDGDHFERQLFLCRREIERKTKGLNGFYMPTFSSRLISYKGLAMPAALRAFYGDLQDSDFETAISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVEGNRNWMASREEFFSHPIWGDVDIKLLHDLMNEHESDSASLDHALEALVLSGRSLEHAMCMLVPPAYRNDEDISDDLRAFYQFIRSFSEPWDGPAGLVYTDGTKLCASLDRNGLRPSRYKLTEDGLLYIGSEAGAVIIDDAKVIRKGRLGPGQMFSVDTSTGQVKNDREIKEALAKTKPYRQWIDENRLELRKFCSPEALAPAEDFNTLALSRQQVTHGISLEELDMVFPPMIKGAQEAVFSMGDDIPLAVLSTYPRLLFTYFKQRFAQVTNPPIDPIREWAVMSASAGLGPERNLLDETAEHCKIINLESAVLFEHQVDRLKHLHDEGFPAAVLDITWPVSAGNAGLKERLDELCKEVEGAVDKGVSIVILSDRRATAERVPIPAILATGAVHHHLNRIRKRMRCSLILESGEVRDTHQISCAFGFGATAVCPYLGFATVRQLVAADAGKGKL-DGISVEKAMANYAKALEKGVLKIMSKMGISVLNSYQGAQIFEAIGIGKDVVDFAFTGVQSKVGGISFAEIAEESLIRHQAAFKTEVPAGETLDLGDPGYNRYRKSGERHAWTTDVIKNFHTFVKSGKEEDYDDYVKVSLETKPVAIKDLFEFVPSASGPIPIEEVESVESIRRRFTTAAMSLGALSPEAHETLAIAMNRIGGKSDSGEGGEDSVRFKPYPNGDFARSWIKQVASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGQKVNALIARLRHTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRARVTVKLVAETGVGTVAAGVAKASADTILISGHDGGTGASPLSSTKHAGSPWELGLAEAQQTLLLNNLRDRVIVRTDGGLRNGKDIVIAAALGAEEYNFGTIAMIAMGCVYVRKCHLNNCPVGIATTDPKFRAKFKGTPEMVINFFNGVAHEARGIMAKLGVRTMDELIGRPE-YLKQRHVPEHPKANLIDLAPILKDVIPELAKHQGVEEASISRICKKERNDGISKGPLDVVVLRDLAKELGTGEVSAETPEYGPNATPDSITAAIMALPDRQPVELSYDVVNTDRNIGTRLSGRIAEIHGDRGFNGHTAVTLKLRGSAGQSLGTFLVSGV 1362
BLAST of hypothetical protein vs. nr
Match: gi|1353606074|ref|WP_105043619.1| (glutamate synthase large subunit [Rubritalea profundi] >gi|1350983044|gb|PQJ29126.1| glutamate synthase subunit alpha [Rubritalea profundi]) HSP 1 Score: 1935.61 bits (5013), Expect = 0.000e+0 Identity = 917/1341 (68.38%), Postives = 1086/1341 (80.98%), Query Frame = 0
Query: 4 YHPDHTPDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPADIGVGVFFLP---EGISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAG-VALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGD----------DAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
Y TPD++G+LH ++NE NCGMGAI + G+ S +I+D A+ SVCNMTHRGAVDAD KTGDGSG+L QIP LFA+ A G DI VGVFFLP E A +++ E + R I +GWR PV P+ELG+LA+ T+P + H L+S+P+ + FER L+L RR +E + A FY+PT SSRLISYKGLAMPATL+AFY D+QD DF T I+LYHQRFSTNTFPAWPLGQPFRM+ HNGEINTVRGNRNW+ASREE FES++WGDD+ L++L + ESDSASLDHALELL LSGRS+EH+MCMLVPPAF ND++ISEE++ FY Y SFSEPWDGPAGL +TDG K+CA LDRNGLRPSR+ LTEDGLLYIGSE GAI + ++ I+ +GRLGPGQML A+ G D+EIKE LA Q PY RWVDEN+V L+ + S VP DF ++ R Q+ +G++ EDL MVFPPM+KGAQEAV+SMG+DIPLA LS YPRLL+TYF+QLFAQVTNPPIDPIREWAVM+L AGLG+E+NLL ETPEHA+++ LESAIL EQELE I E+GFSS+ +DTTWP SEG G+ +A+ R+CA+AE A + I+ILSDRA S +RV IP+LL TG+VHHHLNR RKRL +SLV+DT EARDTHQIACLFGFGATAVCPYLGY+TVRQV+A+D KGKLGDD+TPEVGM NYRKALEKG+LKIMSKMGISVLNSYQ AQIFEA+G+G +V++ FTG SRI GVGFAEIA ES++RH++A D+ G L+LGDPGYNRYRK+GE HA+TTDVIKN HTFVR+GKKEDYDDYV+ ++E P+ IKD+ F PA+G ++++EVEP+E+I +RFTTAAMSMGALSPEAHETLAIAMNR+G KSDSGEGGED RFK YPNGD A+S IKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVN +IARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKE+NPRAKVTVKLV+ +GVGTVA+G AKASAD ILISGHDGGTAASPLSSTKH G+PWELG++EA Q L+ NNLR VTLRTDGG++ G+D++ AAILGAE+ NFGTIAMIAMGCVYVRKCHLNNCPVG+ATT PKWRAKFKGTP+ V+ + V++EARE+MASLG++ +++L+G P+ +L+QREVP+HPKAN +DLS VL D + + RIC +RNDG KPALDL+IL DL A G + LMDR P SY VVNTDRNIGTR +GRVAE++EN GLPA SI+LT GSAGQSFGTFL GG+
Sbjct: 6 YDQRFTPDTTGSLHSFTNERDNCGMGAIVQIHGKASKNILDLAVESVCNMTHRGAVDADMKTGDGSGILSQIPRPLFAREAKKLGSDAAAEDIAVGVFFLPRDNESCHAEIQALAEKTVKERGINIIGWRVCPVAPEELGELAQKTRPHVLHFLMSKPSGWEELQFERQLYLARRTIEIATAETAGFYMPTFSSRLISYKGLAMPATLKAFYLDMQDDDFHTAIALYHQRFSTNTFPAWPLGQPFRMVAHNGEINTVRGNRNWLASREELFESDIWGDDVALLKDLTSDNESDSASLDHALELLALSGRSIEHSMCMLVPPAFINDKDISEELQGFYKYHSSFSEPWDGPAGLVYTDGTKLCASLDRNGLRPSRYVLTEDGLLYIGSEVGAIQVDETSIVAKGRLGPGQMLLANLADGTLTKDREIKEGLAKQAPYRRWVDENQVNLRDYISPEPQVPAVDFEETEIKRLQIMNGISDEDLAMVFPPMVKGAQEAVYSMGNDIPLAILSTYPRLLYTYFQQLFAQVTNPPIDPIREWAVMSLEAGLGSERNLLGETPEHAKIINLESAILLEQELEKIKQMDEYGFSSIQLDTTWPISEGADGMLQAVQRVCAQAEDAADAGTAIVILSDRAASPERVAIPALLITGAVHHHLNRVRKRLSSSLVVDTAEARDTHQIACLFGFGATAVCPYLGYATVRQVIATDTKGKLGDDMTPEVGMKNYRKALEKGILKIMSKMGISVLNSYQSAQIFEAIGIGNDVIDTCFTGTKSRIAGVGFAEIAAESIIRHQSAHS-DLIDGTELELGDPGYNRYRKTGERHAITTDVIKNFHTFVRNGKKEDYDDYVQASLETGPVAIKDLFEFVPPASGAISIDEVEPIEDIRRRFTTAAMSMGALSPEAHETLAIAMNRIGAKSDSGEGGEDPIRFKKYPNGDLAKSRIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNGIIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEINPRAKVTVKLVSSAGVGTVASGVAKASADVILISGHDGGTAASPLSSTKHCGLPWELGLAEAQQTLLANNLRTKVTLRTDGGMKNGKDIVTAAILGAEEYNFGTIAMIAMGCVYVRKCHLNNCPVGVATTDPKWRAKFKGTPEQVINFFNAVSQEAREVMASLGIKKLDDLIGHPE-YLRQREVPEHPKANLIDLSTVLKDMTPEISKMTGTPVSEIARICKQDRNDGIDKPALDLQILKDL----YAKIGSEDLSVLMDRAPVNLSYNVVNTDRNIGTRLSGRVAEVFENRGLPAGSINLTINGSAGQSFGTFLAGGV 1340
BLAST of hypothetical protein vs. nr
Match: gi|517800694|ref|WP_018970902.1| (glutamate synthase large subunit [Rubritalea marina]) HSP 1 Score: 1897.48 bits (4914), Expect = 0.000e+0 Identity = 919/1351 (68.02%), Postives = 1086/1351 (80.38%), Query Frame = 0
Query: 4 YHPDHTPDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGDPAD-IGVGVFFLPEG---ISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPG----KTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAG-VALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKAGDDA----------IRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGILSTVS 1335
Y TPD++G+LH +SNE NCGMGAIAN+ G S+DI++ A+ SVCNMTHRG VDAD KTGDGSG+L QIP LF + A G T + AD +GVGVFF P + N++ E L R I F+GWR+VPV DELG A+ + P+I HL+V++P+ + HFER L+LVRR +E + A FYIPTLS+RLISYKGLAMPATLRAFY D+Q+ DF+T I++YHQRFSTNTFPAWPLGQPFRMM HNGEINTVRGNRNWMASREEFF SEVWG+DI ++NL+++ ESDSASLDH LELL LSGR +EH+MCMLVPPA+RND EISEEV FY Y SF+EPWDGPAGL FTDG KICAGLDRNGLRPSR+ L +DGLLY+GSE G I ++K I +GRLGPGQ + A+T TGE D+E+KERLA Q PY RWVDEN+V+LK + SH A+VP ED++A+ LSR Q+ + ++ E+LDMVFPPMIKGAQEAVFSMG DIPLA LS YPRLL TYFKQLFAQVTNPPIDPIREWAVM+L AGLGAE+NLL E+PEHARVL LESAIL E E++ I EHGF++ +IDTTW ++G AGLK A++ +CAEAE AV++ ++++ILSD+A +RV IPSL+ TG+VHHHLNR +KR+R S+V++T EARDTH +A LFGFGATAVCPYLGY+TVRQVVA+D KGKLG+ +TPE M NYRKALEKGLLKIMSKMGISVLNSYQGAQIFEA+G+GKEV++ FTG SRIGG+GFA+IAEES++RH+AA++ +E G + L LGDPGYNRYRKSGE HA+TTD IKN HTFV+ GK EDY +YVK +++ PITIKD+L F + G + +EEVEP+E I RFTTAAMSMGALSPEAHETLAIAMNR+G KSDSGEGGED RF YPNGD ARS IKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVN +IARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNP AKVTVKLV+E+GVGT+AAG AKA ADNILISGHDGGTAASPLSSTKH G+PWELG++EA Q L+MNNLR+ +TLRTDGG++ G+D++ AAILGAEQ NFGT AMIAMGCVYVRKCHLNNCPVG+ATT PKWR+KFKGTP+ V+ + GVA+EAREIMA LG+R +++L+GRP+ FL+QR VP+HPKAN+LDLS VL D + A I ERNDG HKP LD++I+ D+ A+ A L DR P Y V+T+RN+GTRT+G VA + GLPA SI L F GSAGQS GTFL GI V+
Sbjct: 6 YDQRFTPDTTGSLHSFSNERDNCGMGAIANINGVPSFDILNKAVESVCNMTHRGGVDADMKTGDGSGILSQIPRKLFKQEAEKLGATVENADDLGVGVFFFPNNNAEAAQNIQELAEKTLAERGITFIGWRQVPVAKDELGVQAQKSCPDIQHLIVTKPSGWEDLHFERQLYLVRRTIEHATSDVADFYIPTLSARLISYKGLAMPATLRAFYLDMQNPDFETAIAVYHQRFSTNTFPAWPLGQPFRMMAHNGEINTVRGNRNWMASREEFFNSEVWGEDIELVKNLVKDNESDSASLDHCLELLTLSGRDIEHSMCMLVPPAYRNDNEISEEVYDFYQYHSSFAEPWDGPAGLVFTDGVKICAGLDRNGLRPSRYALFDDGLLYVGSEIGCIECDENKAIAKGRLGPGQTMVANTATGELELDREVKERLAKQAPYGRWVDENQVQLKDYVSHEAIVPAEDYDAVTLSRMQLANAISDEELDMVFPPMIKGAQEAVFSMGCDIPLAALSHYPRLLQTYFKQLFAQVTNPPIDPIREWAVMSLEAGLGAEKNLLNESPEHARVLNLESAILLEHEMDRIKEMGEHGFNNRIIDTTWALADGGAGLKAAVEAVCAEAEKAVDEGVDVIILSDKAADIERVAIPSLMITGAVHHHLNRVKKRMRASIVVETAEARDTHTVAMLFGFGATAVCPYLGYATVRQVVANDAKGKLGEGMTPEKAMTNYRKALEKGLLKIMSKMGISVLNSYQGAQIFEAVGIGKEVIDLCFTGCVSRIGGIGFADIAEESIIRHKAAYKDIIEDGAAVTEALPLGDPGYNRYRKSGERHAITTDYIKNFHTFVKTGKAEDYAEYVKASLDTHPITIKDLLEFAPSSNGPIPIEEVEPIETIRTRFTTAAMSMGALSPEAHETLAIAMNRIGAKSDSGEGGEDPVRFTEYPNGDLARSAIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNGIIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPYAKVTVKLVSEAGVGTIAAGVAKADADNILISGHDGGTAASPLSSTKHTGLPWELGLAEAQQTLVMNNLRSKITLRTDGGMKNGKDIVTAAILGAEQFNFGTTAMIAMGCVYVRKCHLNNCPVGVATTDPKWRSKFKGTPEMVINFFNGVAQEAREIMAQLGIRKLDDLIGRPE-FLKQRHVPEHPKANSLDLSPVLKDVVAETARAFDLDPSEVSLINVDERNDGIHKPELDVQIIEDIKSKQNIAQ----FAELADRAPIALDYKAVSTNRNLGTRTSGAVAMAFGKAGLPAGSIQLNFTGSAGQSLGTFLSPGIEINVT 1351
BLAST of hypothetical protein vs. nr
Match: gi|740321755|ref|WP_038158901.1| (glutamate synthase large subunit [Verrucomicrobium sp. BvORR106]) HSP 1 Score: 1594.33 bits (4127), Expect = 0.000e+0 Identity = 799/1342 (59.54%), Postives = 985/1342 (73.40%), Query Frame = 0
Query: 2 HLYHPDHTPDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGD-PADIGVGVFFLPEGISAN--VKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKE--AAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASD--VKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAG--VALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLAD---KAGDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTK-SYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
H Y+ + P+ GTLH E CG+G +A + G++S I+DYAL C + HRGA++AD KTGDG+GVL QIPH L FG D D+GVG+FFLP+ +A ++ ESVL +R++ +GWR+VPVN ELG+ AR T P I+ LLV RP ++D A FER LFL RRE+E + ++ A FY+P++S R + YK L + L FY DLQD +++T ++++HQRFSTNTFP W L PFRM+ HNGEINTVRGNRNWM SR FE W D H L+ L +SDSASLD ALELLVLSGRS+ +M MLVPPA+ D +EE +++Y Y FSEPWDGPAGL FTDGR I A LDRNGLRPSR+ LTEDG+ +GSE G + L D+K+I++GRL PG+M+S D G YD+EIK++L +++PY W+ E RVEL + HA P ED + L LS++QVT G E+++M F PM+ +EA++SMGDD L+ LS+ P+LLFTYFKQLFAQVTNPPIDPIRE VM+L LG ++N L ETPEHA V+ L S LFE ELET+ K+ F V+D T+P SEG GLKKA+DR+CAEAE AVND I ILILSDRA RVP+PSL+ATG+VHHHLNR + R+R SLV+D+GEARDTHQ+A LFGFGA+AVCPYL + TV++V+ D + + + + YRK LEKG+LKIMSKMGISVL+SY GAQIFEA+G+GKE+++ FTG S+I G+GF E+AEESL RH A + V ++L DPG+ R R+ GE HA+T VIKN HTFV+ GK EDY+ YVK +EN P+ +KD++ F VP++ + LEEVEP+E+I RFTTAAMS+GA+SPEAHE LAIAMN +GGKSDSGEGGED RRFKPY NGD+A S IKQ+ASGRFGV+A YL NA ELEIKMAQGAKPGEGGQLP KVN LIARLRNTQPGV LISPPPHHDIYSIEDLAQLIHDLKEVNPRA+V VKLVAESGVGTVAAG AKA+AD ILISGHDGGT ASPLSS KHAG+PWELG++EA QVLM+N LR+ VTLRTDGGLR GRD+ +AAILGAE+ NFGTIA+IA+GCVYVR+CHLNNCPVG+ATT PK+R KFKG P+HVV + VA E R+IMA LG+ +N+L+GRP+ FL+QR VPDH KAN LDLS +L D +A +D RIC N+ + LD +IL Q + D+ SY VVNT+RNIGT +G +A + NHGLP +I++ GSAGQSFGTFLCGG+
Sbjct: 4 HPYYDEPIPNE-GTLHNMDMERDACGVGFVAQVDGKRSRKILDYALGGCCAVVHRGAMEADMKTGDGAGVLTQIPHKLLLPEVEKFGVKLDHELDLGVGMFFLPQDETARLRIQLVAESVLDKRDVTVIGWRKVPVNERELGEKARRTMPFITQLLVRRPEDLDDAAFERALFLSRREIELKGKQQGLAEFYVPSMSHRTVIYKALVVATALEKFYYDLQDENYETALAVFHQRFSTNTFPTWALSHPFRMLAHNGEINTVRGNRNWMGSRASDFEHPWWEGDEHLLKGLCDPKQSDSASLDAALELLVLSGRSVTESMSMLVPPAYGIDPTTTEEEKSYYEYHSCFSEPWDGPAGLIFTDGRVIAATLDRNGLRPSRYKLTEDGVFALGSEVGIVPLDDAKVIKKGRLSPGEMISVDISKGVVSYDEEIKQQLVSRQPYGEWLKEQRVELTAQAPHA---PKEDLDILSLSQKQVTFGWNKEEVEMAFAPMLAKGEEAIYSMGDDAALSILSKQPKLLFTYFKQLFAQVTNPPIDPIRERGVMSLDVVLGWQRNWLAETPEHAHVVHLTSPFLFENELETLKGLKD--FPCRVLDITFPVSEGPEGLKKAVDRLCAEAEQAVNDDIRILILSDRAVDHTRVPVPSLMATGAVHHHLNRKQVRMRLSLVVDSGEARDTHQMALLFGFGASAVCPYLAFETVQEVLEKDKTARKPILEGFDFAKALTYYRKGLEKGVLKIMSKMGISVLSSYTGAQIFEAVGLGKELMDKCFTGSPSQISGIGFNEVAEESLARHAAGYGPAVPEAGAIELADPGFYRPRREGEVHAVTGPVIKNFHTFVKSGKSEDYESYVKTQLENTPVALKDLMEF-VPSSDGPIPLEEVEPIEDIRVRFTTAAMSLGAISPEAHEALAIAMNSIGGKSDSGEGGEDPRRFKPYENGDWAMSKIKQVASGRFGVTAEYLANAWELEIKMAQGAKPGEGGQLPAMKVNRLIARLRNTQPGVTLISPPPHHDIYSIEDLAQLIHDLKEVNPRARVCVKLVAESGVGTVAAGVAKANADIILISGHDGGTGASPLSSIKHAGLPWELGLAEAQQVLMLNGLRDRVTLRTDGGLRNGRDIAMAAILGAEEFNFGTIALIALGCVYVRQCHLNNCPVGVATTDPKFRQKFKGKPEHVVNFFNSVAHETRQIMAQLGIAKMNDLIGRPE-FLRQRHVPDHKKANLLDLSRILRDVGKEADEDLPRICRVNSNERINNHPLDDKIL------------QAAQVAISDKRKIKPLSYKVVNTNRNIGTGLSGEIAFHHGNHGLPDGTIEVNLEGSAGQSFGTFLCGGV 1325
BLAST of hypothetical protein vs. nr
Match: gi|497650855|ref|WP_009965039.1| (glutamate synthase large subunit [Verrucomicrobium spinosum]) HSP 1 Score: 1586.62 bits (4107), Expect = 0.000e+0 Identity = 793/1342 (59.09%), Postives = 987/1342 (73.55%), Query Frame = 0
Query: 2 HLYHPDHTPDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGD-PADIGVGVFFLPEGISAN--VKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKE--AAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASD--VKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAG--VALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLAD---KAGDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTK-SYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
H Y+ + P+ GTLH E CG+G +A + G++S I+DYAL C + HRGA++AD KTGDG+GVL QIPH L FG + D+GVG+FFLP+ +A ++ ESV+ +R++ +GWR+VPVN ELG+ AR T P I+ LLV RP ++D A FER+LFL RRE+E + ++ A FYIP++S R + YK L + L FY DLQD +++T ++++HQRFSTNTFP W L PFRM+ HNGEINTVRGNRNWMASR FE W D H L+ L +SDSASLD ALELLVLSGRS+ +M MLVPPA+ D +EE +++Y Y FSEPWDGPAGL +TDGR I A LDRNGLRPSR+ LTEDG+ +GSE G + L D+K+I++GRL PG+M+S D G Y++EIK++L +++PY W+ E RVEL + HA P ED + L LS++QVT G E+++M F PM+ +EAV+SMGDD L+ LS+ P+LLFTYFKQLFAQVTNPPIDPIRE VM+L LG ++N L ETPEHA V+ L S LFE ELET+ K+ F V+D T+P SEG GLKKA+DR+C+EAE AVND I ILILSDRA RVP+PSL+ATG+VHHHLNR + R+R SLV+D+GEARDTHQ+A LFGFGA+AVCPYL + TV++V+ D + + + + YRK LEKG+LKIMSKMGISVL+SY GAQIFEA+G+GKE+++ FTG S+I G+GF E+AEESL RH A + V ++L DPG+ R R+ GE HA+T VI+N HTFV+ GK EDY+ YVK +EN P+ +KD++ F VP++ + L+EVEP+E+I RFTTAAMS+GA+SPEAHE LAIAMN +GGKSDSGEGGED RRFKP+ NGD+A S IKQ+ASGRFGV+A YL NA ELEIKMAQGAKPGEGGQLP KVN LIARLRNTQPGV LISPPPHHDIYSIEDLAQLIHDLKEVNPRA+V VKLVAESGVGTVAAG AKA+AD ILISGHDGGT ASPLSS KHAG+PWELG++EA QVLM+N LR+ VTLRTDGGLR GRD+ +AAILGAE+ NFGTIA+IA+GCVYVR+CHLNNCPVG+ATT PK+R++FKG P+HVV + VA E R+IMA LG+ +N+L+GRP+ FL+QR VPDH KAN LDLS +L D +A +D RIC N+ + LD +IL Q + D+ SY VVNT+RNIGT +G +A + NHGLP +I + GSAGQSFGTFLCGG+
Sbjct: 4 HPYYDEPIPNE-GTLHNMDMERDACGVGFVAQVDGKRSRKILDYALGGCCAVVHRGAMEADMKTGDGAGVLTQIPHKLLLPEVEKFGVKLEHELDLGVGMFFLPQDETARLRIQLVAESVVDKRDVTVIGWRKVPVNERELGEKARRTMPFITQLLVRRPEDMDDAAFERVLFLARREIELKGKQQGLADFYIPSMSHRTVIYKALVVATALEKFYYDLQDENYETALAVFHQRFSTNTFPTWALSHPFRMLAHNGEINTVRGNRNWMASRASDFEHPWWEGDEHLLKGLCDPKQSDSASLDAALELLVLSGRSVTESMSMLVPPAYGIDPTTTEEEKSYYEYHSCFSEPWDGPAGLIYTDGRVIAATLDRNGLRPSRYKLTEDGVFALGSEVGIVPLDDAKVIKKGRLSPGEMISVDISKGVVTYNEEIKQQLVSRQPYGEWLTEQRVELTAQAPHA---PKEDLDILGLSQKQVTFGWNKEEVEMAFAPMLARGEEAVYSMGDDAALSILSKQPKLLFTYFKQLFAQVTNPPIDPIRERGVMSLDVVLGWQRNWLAETPEHAHVVHLTSPFLFENELETLKGLKD--FPCRVLDITFPVSEGPEGLKKAVDRLCSEAEQAVNDDIRILILSDRAVDHTRVPVPSLMATGAVHHHLNRKQVRMRLSLVVDSGEARDTHQMALLFGFGASAVCPYLAFETVQEVLEKDKTARKPILEGFDFAKALTYYRKGLEKGVLKIMSKMGISVLSSYTGAQIFEAVGLGKELMDKCFTGSPSQIAGIGFNEVAEESLARHAAGYGTAVPEAGAIELADPGFYRPRREGEMHAVTGPVIRNFHTFVKSGKPEDYESYVKAQLENTPVALKDLMEF-VPSSDGPIPLDEVEPIEDIRVRFTTAAMSLGAISPEAHEALAIAMNSIGGKSDSGEGGEDPRRFKPFENGDWAMSKIKQVASGRFGVTAEYLANAWELEIKMAQGAKPGEGGQLPAMKVNRLIARLRNTQPGVTLISPPPHHDIYSIEDLAQLIHDLKEVNPRARVCVKLVAESGVGTVAAGVAKANADIILISGHDGGTGASPLSSIKHAGLPWELGLAEAQQVLMLNGLRDRVTLRTDGGLRNGRDIAMAAILGAEEFNFGTIALIALGCVYVRQCHLNNCPVGVATTDPKFRSRFKGKPEHVVNFFNSVAHEVRQIMAQLGIAKMNDLIGRPE-FLRQRHVPDHKKANLLDLSRILRDVGKEADEDLPRICRVNSNERINNHPLDDKIL------------QAAQVAISDKRKIKPLSYKVVNTNRNIGTGLSGEIAFHHGNHGLPDGTIQVNLEGSAGQSFGTFLCGGV 1325
BLAST of hypothetical protein vs. nr
Match: gi|910224978|ref|WP_050030639.1| (glutamate synthase large subunit [Verrucomicrobium sp. BvORR034]) HSP 1 Score: 1584.31 bits (4101), Expect = 0.000e+0 Identity = 794/1344 (59.08%), Postives = 985/1344 (73.29%), Query Frame = 0
Query: 2 HLYHPDHTPDSSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGD-PADIGVGVFFLPEGISAN--VKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRREVEREFTKE--AAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASDVKGKL----GDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDVEPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAG--VALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLAD---KAGDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTK-SYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGI 1330
H Y+ + P+ GTLH E CG+G +A + G++S I+DYAL C + HRGA++AD KTGDG+GVL QIPH L FG + D+GVG+FFLP+ +A ++ ESV+ +R++ +GWR+VPVN ELG+ AR T P I+ LLV RP ++D A FER LFL RRE+E + ++ A FY+P++S R + YK L + L FY DLQD +++T ++++HQRFSTNTFP W L PFRM+ HNGEINTVRGNRNWMASR FE W D H L+ L +SDSASLD ALELLVLSGRS+ +M MLVPPA+ D +EE +++Y Y FSEPWDGPAGL +TDGR I A LDRNGLRPSR+ LTEDG+ +GSE G + L D+K+I++GRL PG+M+S D G Y++EIK++L +++PY W+ E RVEL + HA P ED + L LS++QVT G E+++M F PM+ +EAV+SMGDD L+ LS+ P+LLFTYFKQLFAQVTNPPIDPIRE VM+L LG ++N L ETPEHA V+ L S LFE ELET+ K+ F V+D T+P SEG GLKKA+DR+C+EAE AVND I ILILSDRA RVP+PSL+ATG+VHHHLNR + R+R S+V+D+GEARDTHQ+A LFGFGA+AVCPYL + TV++V+ D + G D + YRK LEKG+LKIMSKMGISVL+SY GAQIFEA+G+GKE+++ FTG S+I G+GF E+AEES RH A + V ++L DPG+ R R+ GE HA+T VIKN HTFV+ GK EDY+ YVK +EN P+ +KD++ F VP++ + L+EVEP+E+I RFTTAAMS+GA+SPEAHE LAIAMN +GGKSDSGEGGED RRFKP+ NGD+A S IKQ+ASGRFGV+A YL NA ELEIKMAQGAKPGEGGQLP KVN LIARLRNTQPGV LISPPPHHDIYSIEDLAQLIHDLKEVNPRA+V VKLVAESGVGTVAAG AKA+AD ILISGHDGGT ASPLSS KHAG+PWELG++EA QVLM+N LR+ VTLRTDGGLR GRD+ +AAILGAE+ NFGTIA+IA+GCVYVR+CHLNNCPVG+ATT PK+R KFKG P+HVV + VA E R+IMA LG+ +N+L+GRP+ FL+QR VPDH KAN LDLS +L D +A +D RIC N+ + LD +IL Q + D+ SY VVNT+RNIGT +G +A + NHGLP +I++ GSAGQSFGTFLCGG+
Sbjct: 4 HPYYDEPIPNE-GTLHNMDMERDACGVGFVAQVDGKRSRKILDYALGGCCAVVHRGAMEADMKTGDGAGVLTQIPHKLLLPEVEKFGVKLEHELDLGVGMFFLPQDETARLRIQLVAESVVDKRDVTVIGWRKVPVNERELGEKARRTMPFITQLLVRRPEDMDDAAFERALFLARREIELKGKQQGLADFYVPSMSHRTVIYKALVVATALEKFYYDLQDENYETALAVFHQRFSTNTFPTWALSHPFRMLAHNGEINTVRGNRNWMASRASDFEHPWWEGDEHLLKGLCDPKQSDSASLDAALELLVLSGRSVTESMSMLVPPAYGIDPTTTEEEKSYYEYHSCFSEPWDGPAGLIYTDGRVIAATLDRNGLRPSRYKLTEDGVFALGSEVGIVPLDDAKVIKKGRLSPGEMISVDIAKGVVTYNEEIKQQLVSRQPYGEWLKEQRVELTAQAPHA---PKEDLDILSLSQKQVTFGWNKEEVEMAFAPMLARGEEAVYSMGDDAALSILSKQPKLLFTYFKQLFAQVTNPPIDPIRERGVMSLDVVLGWQRNWLAETPEHAHVVHLTSPFLFENELETLKGLKD--FPCRVLDITFPVSEGPEGLKKAVDRLCSEAEQAVNDDIRILILSDRAVDHTRVPVPSLMATGAVHHHLNRKQVRMRLSIVVDSGEARDTHQMALLFGFGASAVCPYLAFETVQEVLEKDKTARKPILEGFDFAK--ALTYYRKGLEKGVLKIMSKMGISVLSSYTGAQIFEAVGLGKELMDKCFTGSPSQIAGIGFNEVAEESFARHAAGYGTAVPEAGAIELADPGFYRPRREGEVHAVTGPVIKNFHTFVKSGKSEDYETYVKAQLENTPVALKDLMEF-VPSSDGPIPLDEVEPIEDIRVRFTTAAMSLGAISPEAHEALAIAMNSIGGKSDSGEGGEDPRRFKPFENGDWAMSKIKQVASGRFGVTAEYLANAWELEIKMAQGAKPGEGGQLPAMKVNRLIARLRNTQPGVTLISPPPHHDIYSIEDLAQLIHDLKEVNPRARVCVKLVAESGVGTVAAGVAKANADIILISGHDGGTGASPLSSIKHAGLPWELGLAEAQQVLMLNGLRDRVTLRTDGGLRNGRDIAMAAILGAEEFNFGTIALIALGCVYVRQCHLNNCPVGVATTDPKFRQKFKGKPEHVVNFFNSVAHEVRQIMAQLGIAKMNDLIGRPE-FLRQRHVPDHKKANLLDLSRILRDVGKEADEDLPRICRVNSNERINNHPLDDKIL------------QAAQVAISDKRKIKPLSYKVVNTNRNIGTGLSGEIAFHHGNHGLPDGTIEVNLEGSAGQSFGTFLCGGV 1325
BLAST of hypothetical protein vs. nr
Match: gi|1154905491|ref|WP_078811340.1| (glutamate synthase large subunit [Prosthecobacter debontii] >gi|1152229876|emb|SKA75952.1| glutamate synthase (NADPH/NADH) large chain [Prosthecobacter debontii]) HSP 1 Score: 1563.9 bits (4048), Expect = 0.000e+0 Identity = 781/1350 (57.85%), Postives = 976/1350 (72.30%), Query Frame = 0
Query: 3 LYHPDHTPD--SSGTLHEWSNETANCGMGAIANLRGEKSYDIIDYALTSVCNMTHRGAVDADQKTGDGSGVLCQIPHALFAKAAANFGFTGD-PADIGVGVFFLP--EGISANVKSFTESVLTRRNIPFLGWREVPVNPDELGDLARLTQPEISHLLVSRPTEVDAAHFERLLFLVRRE--VEREFTKEAAFYIPTLSSRLISYKGLAMPATLRAFYSDLQDSDFKTGISLYHQRFSTNTFPAWPLGQPFRMMCHNGEINTVRGNRNWMASREEFFESEVWGDDIHFLRNLMREGESDSASLDHALELLVLSGRSLEHAMCMLVPPAFRNDQEISEEVRAFYNYIRSFSEPWDGPAGLCFTDGRKICAGLDRNGLRPSRFTLTEDGLLYIGSESGAIILPDSKIIRRGRLGPGQMLSADTQTGEFLYDKEIKERLAAQKPYFRWVDENRVELKSFTSHAALVPTEDFNALDLSRQQVTHGVTAEDLDMVFPPMIKGAQEAVFSMGDDIPLAPLSRYPRLLFTYFKQLFAQVTNPPIDPIREWAVMTLGAGLGAEQNLLTETPEHARVLALESAILFEQELETITNRKEHGFSSLVIDTTWPASEGEAGLKKALDRICAEAETAVNDKIEILILSDRATSADRVPIPSLLATGSVHHHLNRCRKRLRTSLVIDTGEARDTHQIACLFGFGATAVCPYLGYSTVRQVVASD--VKGKLGDDITPEVGMANYRKALEKGLLKIMSKMGISVLNSYQGAQIFEALGVGKEVVNFAFTGVHSRIGGVGFAEIAEESLVRHRAAFQVDV----EPGKTLDLGDPGYNRYRKSGEAHALTTDVIKNLHTFVRDGKKEDYDDYVKVTIENDPITIKDILAFKVPAAG--VALEEVEPVEEIVKRFTTAAMSMGALSPEAHETLAIAMNRLGGKSDSGEGGEDARRFKPYPNGDFARSYIKQIASGRFGVSAHYLVNADELEIKMAQGAKPGEGGQLPGHKVNALIARLRNTQPGVQLISPPPHHDIYSIEDLAQLIHDLKEVNPRAKVTVKLVAESGVGTVAAGCAKASADNILISGHDGGTAASPLSSTKHAGIPWELGISEAHQVLMMNNLRNNVTLRTDGGLRTGRDVIIAAILGAEQMNFGTIAMIAMGCVYVRKCHLNNCPVGIATTSPKWRAKFKGTPDHVVTYMMGVAEEAREIMASLGVRSINELVGRPQKFLQQREVPDHPKANTLDLSAVLADKA---GDDAIRICTHERNDGFHKPALDLEILADLAEHTAAAEGQNPTAGLMDRGPFTKSYTVVNTDRNIGTRTAGRVAEIYENHGLPANSIDLTFRGSAGQSFGTFLCGGILSTV 1334
+ HP + D + G+LH E CG+G +AN+ G++S DI++ A+ VCN+ HRGAVDAD+ TGDG+GVL QIPH + G + D+ VGVFFLP E ++ E +L R+I LGWR+VPVNP+ELG+ AR T P I HL + RP +D FER L+LVRRE ++ + K A FYI ++S R I YK L +P++L FY+DLQ D++T ++L+HQRFSTNTFP W L PFRM+ HNGEINTVRGNRNW++SR FE EVW + L++L+ SDSASLD ALELLVLSGRSL HAM MLVP A+ D S +++AFY Y FSEPWDGPA + T+G + A LDRNGLRPSR+ LTEDG+ +GSE G I + D+K++++GRL PG+ML D G+ +++EIK LA+++PY W+ K+ T+ + P E+ + L LS++Q +G T E++D PM++ +E ++SMGDD L+ LS PRLL TYFKQLFAQVTNPPIDPIRE AVM+L LG ++N L ETPEHA V+ L S LFE EL I + F V+DTTWP +EG AG+KKALDR+C EAE AVND++ ILILSDRA R IP+LLATG+VHHHLNR + R+R SLV+DTGEARDTHQ+ACLFGFGA+AVCPYL + T+++V+ +D + + + + + N+RK+LEKG+LKIMSKMGISVL+SY GAQIFEA+G+G +V+ +AFTG S+I G+GF EIAEE+L RH F V E +DLGDPGY R R+ GE HA+T VIKN HTFV+ G EDY YV+ ++N P+ +KD+L F VP+ + ++EVEP+E+I RFTTAAMS+GA+SPEAHE LAIAMNR+GGKSDSGEGGED +RFKPY NGD+A S IKQ+ASGRFGV+A YL NA ELEIKMAQGAKPGEGGQLP KVN +IARLRNT PGV LISPPPHHDIYSIEDLAQLIHDLKE NPRA+V VKLVAESGVGTVAAG AKA+AD IL+SGHDGGT ASPLSS KHAG+PWELG++E QVLM+N LR VTLRTDGGLR GRD+ IAA+LGAE+ NFGTIA+IA+GCVYVR+CHLNNCPVG+ATT PK+R+KFKG P+HVV + GVA+E REIMA LGV +N+L+GRP+ FL+QREVP H KAN ++LS VL D G DA RIC +NDG + LD ++ D + P Y + NT RNIGT+ +G +A + NHGLP S+D+T GSAGQSFGTFLCGG+ T+
Sbjct: 1 MKHPFYDQDIPNEGSLHRMDLERDACGVGFVANIHGKRSRDILEMAICGVCNVQHRGAVDADRVTGDGAGVLTQIPHKVLMPEVEKMGHKLEHEMDLAVGVFFLPNDETQRLKIQLVAEGILRNRDIKVLGWRDVPVNPNELGEKARRTMPFIQHLFMERPEGMDDNSFERQLYLVRRELRIKAKEAKLAEFYIASMSHRTIVYKALLLPSSLEKFYTDLQSDDYETSLALFHQRFSTNTFPTWALSHPFRMLAHNGEINTVRGNRNWLSSRASDFEHEVWDGEEWLLKDLVDANSSDSASLDQALELLVLSGRSLTHAMGMLVPSAYGIDPTTSADLKAFYEYHECFSEPWDGPAAMVLTNGLSVVASLDRNGLRPSRWKLTEDGVFALGSEVGIIAIDDAKVLKKGRLAPGEMLEVDILKGKVRFNEEIKTALASRQPYGEWLSSR----KNLTAQSPQTPKEELDILSLSQRQAAYGWTKEEIDFSLVPMLQKGEEGIYSMGDDASLSILSTRPRLLTTYFKQLFAQVTNPPIDPIRERAVMSLDVVLGWQRNWLGETPEHASVVHLTSPFLFENELSDI--KALPNFPHRVLDTTWPIAEGPAGMKKALDRLCYEAEAAVNDEVRILILSDRAVDEGRAIIPALLATGAVHHHLNRKQVRMRLSLVVDTGEARDTHQMACLFGFGASAVCPYLTFDTIQEVLENDKTARKPVLEGVEYGKALTNFRKSLEKGVLKIMSKMGISVLSSYTGAQIFEAVGIGSDVMTYAFTGTPSQIEGIGFTEIAEEALARHALGFAQPVPTQEEAASNVDLGDPGYYRPRQKGEMHAVTGPVIKNFHTFVKSGSPEDYSKYVEAQLQNSPVALKDLLDF-VPSGDGPIPIDEVEPIEDIRVRFTTAAMSLGAISPEAHEALAIAMNRIGGKSDSGEGGEDPKRFKPYENGDWANSKIKQVASGRFGVTAEYLANAWELEIKMAQGAKPGEGGQLPAMKVNRMIARLRNTTPGVMLISPPPHHDIYSIEDLAQLIHDLKEANPRARVCVKLVAESGVGTVAAGVAKANADIILVSGHDGGTGASPLSSIKHAGLPWELGLAETQQVLMLNGLRERVTLRTDGGLRNGRDIAIAAMLGAEEFNFGTIALIALGCVYVRQCHLNNCPVGVATTDPKFRSKFKGKPEHVVNFFNGVAQEVREIMAQLGVSKMNDLIGRPE-FLRQREVPGHKKANMINLSRVLKDVGKDLGQDAPRICLMNQNDGLDQHPLDDRLIQDAQFAISDKRKVKPV-----------RYKIKNTFRNIGTKLSGEIAFHHGNHGLPDGSVDVTCEGSAGQSFGTFLCGGVKLTL 1331 The following BLAST results are available for this feature:
BLAST of hypothetical protein vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 1
BLAST of hypothetical protein vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of hypothetical protein vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold5739_size4378:274..4308+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold5739_size4378-processed-gene-0.0 ID=snap_masked-scaffold5739_size4378-processed-gene-0.0|Name=hypothetical protein|organism=Tigriopus kingsejongensis|type=gene|length=4035bp|location=Sequence derived from alignment at scaffold5739_size4378:274..4308+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'hypothetical protein' has the following synonyms
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