BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000010412 (supercontig:LSalAtl2s:LSalAtl2s690:121806:126011:1 gene:EMLSAG00000010412 transcript:EMLSAT00000010412 description:"maker-LSalAtl2s690-augustus-gene-1.7")

HSP 1 Score: 820.076 bits (2117), Expect = 0.000e+0
Identity = 446/1001 (44.56%), Postives = 613/1001 (61.24%), Query Frame = 0

Query:    2 DIPMSQKKKPTVGDFVRTGSCVGEPQANALHCLLNRELTVFTDVLNDTDTENRSQSFPLPDDHWINQAFPEEDDKTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQ-PGIDVNIKDKKNGQTALYMAV-KASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKAANRKGEGPKLLEDFRSLLLQTDSFTLNN-RVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGGRRNDEHFVQDLLD-ILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYL-DDCIEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERV----ALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILF--WNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPNVRKSRCDCLRVELMDDDIIARTKEIVLQK 991
            +IP+   K+P +    R+GSC+G+ + N L C+    LT F+D L   D+    + FPL  DHWIN     ED K+L+++ LEK   +F  +LL+ GA    +N   G  P+H+ + +G+L SL+LLF    N  D N+ MR NGRT LHL  E    E    LL Q P +D  IKDKK GQT L++A  K +   I ++L++ GAD D  C G     +L+               +PP        + + L +I+EK    +G   +LL +F+  L+Q     + + R      L+QK CD  +   +  + N    ++PD  +E   + P+ +AA R     ++ L   K      N  + ETILH LLK G +       D L  IL +  I  QI+ I+N++D+L NTALH A Q   Q VV+ LL LGANIGM N +NE PVT I P T+E +L +DC+ ++GDVNH+ FE+ ++YSFLAPP EDL +        SP D +   +    +D ++    ALPE  +LW M +SK HRHLLKHPV+TSFL+ KW RIR YFNRNLRF++ FVF+ TW+IFE +GG  +++ E   I   +++Y  F   +  FI RD   DI D++ + K  S      S       V+ S+W E   ++ +  ++  GEP L  AL  + + LL RE   ++VS+KRYI+SPEN ++V +II +  +LF        + L+RH +   +VLSWA L+TL+GKHPKL  YNVYVTMFYKV+ TFF+FL WY  FIIAFGLGFYI+LHK+    +  G D YVFF+ P  +++KTSTMFVGELEFSDIPI++D      SY FF+SFVFLIVVVLMNLLNGLAVS TG+IQEKAE V Y+SRVETISYTES+LLGDPF+FLS WP + W+ +IPS S   Q+ +++ +Q+ F+++TGATGILLFY+FLP KKLT++PN + + C C ++  MD  II+  K+IVL K
Sbjct:    3 NIPLKSAKRPLLESSGRSGSCLGDTRINLLECINTCNLTSFSDSL--VDSIAGKEDFPLQPDHWINSPIESEDGKSLMIIVLEKKYSEFVALLLKFGARADHFNESYGLYPIHIPSRNGDLESLKLLFRYLINSADPNSVMRINGRTPLHLMAENNFVEGXEXLLRQKPKVD--IKDKKGGQTPLFLAAFKRADSSIIRSLLKYGADPDLKCLGSKTARDLINRNYDIVLDED--NSSPPESSSKQGVSIQRLGEIVEKLGLGQGNYYQLLNEFQYGLIQYKEEEIADYRFPGNMNLVQKICDQNIEDCLKYLFNNFPNMSPDFTSEG--LSPVCIAAQRMSRPILQILADKKADFTTMNTHSSETILHILLKEGNKRS----HDALKFILNDPEIEQQIRSIMNKKDMLGNTALHLAAQRQDQEVVKRLLQLGANIGMKNIYNEMPVTNIXPSTMEEFLNEDCLLSKGDVNHDDFELEFDYSFLAPPYEDLMY------GLSPHDDDESMIHIKPSDLKIPYKSALPEMNSLWIMAESKEHRHLLKHPVITSFLWCKWGRIRPYFNRNLRFFMTFVFLTTWFIFENYGGSKIKN-EQNYITFCYLVYIVFTGCITGFIFRDLNSDINDMMANMKNGSTGRNCLS-------VLLSSWFEIGLLIVIYTIVILGEPFLTKALFIILVCLLFRELLNLAVSIKRYILSPENLVQVLLIIFMSVLLFDPRRTEKHESLHRHIAGTTIVLSWASLVTLIGKHPKLMRYNVYVTMFYKVMKTFFYFLIWYALFIIAFGLGFYIILHKE----NREGEDSYVFFDTPLLSMIKTSTMFVGELEFSDIPIDIDGPYSTFSYGFFISFVFLIVVVLMNLLNGLAVSXTGIIQEKAETVGYVSRVETISYTESVLLGDPFDFLSNWPKIGWIRSIPSISFLRQMYRNKYLQKFFHRVTGATGILLFYSFLPDKKLTIKPNEKLNGCPCFKIHSMDRSIISSAKKIVLSK 973          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000008528 (supercontig:LSalAtl2s:LSalAtl2s5267:1317:4585:-1 gene:EMLSAG00000008528 transcript:EMLSAT00000008528 description:"maker-LSalAtl2s5267-snap-gene-0.3")

HSP 1 Score: 703.36 bits (1814), Expect = 0.000e+0
Identity = 434/1037 (41.85%), Postives = 594/1037 (57.28%), Query Frame = 0

Query:    3 IPMSQKKKPTVGDFVRTGSCVGEPQANALHCLLNRELTVFTDVLNDTDTENRSQSFPLPDDHWINQAFPEEDDKTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHG-ECVNFLLDQPGID-VNIKDKKNGQTALYMAV-KASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKA---ANRKGEGPKLLEDFRSLLLQTDSFT--LNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGGRRN--DEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDDC-IEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGET----DERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLR-------------------DSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTAL---DGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPNVRKSR--CDCLRVELMDDDIIARTKEIVLQKATPNPEEDR 1000
            +P++  KK   G F RT SC GE + NA+   L ++   F ++L +   + RS        HWIN+       K+ L +ALE    +F  +LL+ GA    YN +LG   +HVA  +GN  +     SL     D+N   R  G + LH  V+     +  N L+    +D ++ +D    +T LY+AV +A +    +AL++ GAD +    GK I  + +    P     +  + N  IV+  S+     L  +L+KA      K    + L  F+ +L +       +N     G T +QK+ + GL ++    LN+E                           C K +  +       +V   E I+H +LK  +R+  D  +      IL ++ +  ++  IIN  D L N  LHYATQ+W Q VV+ LL+LGANIG+ N W E P+ KILP+TLE + D+  ++++ DV +E FE+T++YSFLAPP      + RQ         E+ K AE E     +ER  LPET++LWHM QSK HRHLLKHPV+TSFL+LKW RIR YFNRNLRFYLLFV  LTWYI ERFGG SLR                   DS+ Q +   F ++   + + +  ++RDW  +I    R    K +    +S ++  L     +W+E + +  +  VL+     LW  L  L +LL++RE FQ+ VSLKRYI +PENW+EVSMI L+  ILF  D+         RH +AI ++LSW+E+ITL+ +HP+L  YN+YVTMFYKVL TFFFFL WY FFI AF LGFYIMLHKD P  D+   D+Y FFN PW +LVKTSTMFVGE+EF+DIPIN++S+L  LSYAF LSFVFLI+VVLMNLLNGLAVSDTGLI+ KAEIVS++SRVETISYTE +LLGDPF+FLS WP+ K L+ +P+ + C  L ++   ++I   +TGATGILLFY +L  KKLTL PN R     C  L V+ MD ++I   K  ++ K   N  E R
Sbjct:    9 VPLADMKK---GRFCRTTSCFGELEENAIRSFLQKDKLRFNEILAEVSPKKRSHP-----HHWINEGHQRLKFKSFLHIALEDRNEEFVNLLLRAGARADTYNEVLGVGLLHVAIEAGNYSNF---VSLVKYGADVNLVDR-KGLSPLHYAVQSDGNVDIFNSLIHNEKVDELDAEDLAGRRTPLYLAVVQAKNRYFAEALLKRGADSNITVHGKPI-KQAIQSTWPSLRIPE--KSNSSIVE--SKELLYGLNRLLDKAQMNKRSKESNAQNLLHFKIILQKMSEMVSEINKYNCGGMTFIQKSSEYGLPTFH---LNDE-------------------------DSCRKRIFSTS-----KDVFGGENIIHWILKKSQRDRPDADYQDCASIILLSKDLDDELSLIINDADELGNVPLHYATQIWGQEVVKRLLELGANIGIINQWGERPIDKILPETLEEFFDESGLQSKYDVXNEDFEITFDYSFLAPP------ILRQ---------ETTKRAEDENHNACEERPVLPETESLWHMSQSKNHRHLLKHPVITSFLWLKWQRIRGYFNRNLRFYLLFVVALTWYIVERFGGLSLRKNVGIASNSTSYCSDLSYRDSD-QGLSFWFWIFLTHSFIQIFLMIRDWRYEISTFSRR---KGKPGFGSSVLRLIL----GSWLEAVTLCIIFIVLYFRTAALWSVLTGLIVLLILRELFQLGVSLKRYIFTPENWLEVSMITLVCVILFVPDSYFLEPCETKRHLAAITILLSWSEMITLIARHPRLERYNIYVTMFYKVLKTFFFFLLWYSFFIFAFALGFYIMLHKDIPGYDLKN-DEYTFFNYPWLSLVKTSTMFVGEIEFADIPINVNSKLSPLSYAFLLSFVFLILVVLMNLLNGLAVSDTGLIRAKAEIVSFVSRVETISYTEXLLLGDPFDFLSSWPSFKLLMALPNLACCRLLFQNPFFRKISYNLTGATGILLFYRYLTDKKLTLTPNHRGRMDFCPLLSVKDMDPNVIKSAKAKIIAK---NEAEKR 968          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000010075 (supercontig:LSalAtl2s:LSalAtl2s663:686266:692950:1 gene:EMLSAG00000010075 transcript:EMLSAT00000010075 description:"maker-LSalAtl2s663-augustus-gene-7.9")

HSP 1 Score: 617.846 bits (1592), Expect = 0.000e+0
Identity = 416/1035 (40.19%), Postives = 591/1035 (57.10%), Query Frame = 0

Query:   21 SCVGEPQANALHCLLNRELTVFTDVLNDT-----DTENRSQSFP--LPDDHWINQAFPEEDDKTLLMLALEKDLHD----FTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCFG---------KTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKAANRKGEGPKLLEDFRSLLLQTDSFTLN-NRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELV-QSKVSLDLTNVRTDETILHCLLKG----GRRNDEHFVQDLLDIL-GNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDD-CIEAEGDVN--HEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLR-DSEAQDIPA-----------LFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDS------RLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPA-----LKWL-LNIPSCSLCAQL-SKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPNVRKSRCDCLRVELMDDDIIARTKEIVLQKATPNPEEDR 1000
            SC  +PQ     CL N +LT F+ +L +      +  N   S P  LP   WIN     E+D+ +L LALE +  +    F K LL  GA+   +N     A +HVA  S NL+ ++ L  L+ N+ DI+ T +  G+ ALHL V   +   +  LL +P   +N KD    +T L +A+ A +   T  L+E GA+ +    G         ++ FP+L  DK        +  K  PI       T + L+D+L+K         + L  F+S +   D   ++   V  G TLLQ+A   GL  +V  ++N   + N    ++     P +LAA+RGH   ++ L+ + K  +      + ET+LH +L+     G  +D+   +  L++L  +E   +Q++ +IN++D L NT LH+A + WPQ V   LL+LGANIG+ NH+ E P+++I  +TLE +LD+ C + + D +   + FEVT+ Y FLAPP      V+ + L+    D E Q   E E     ALPET +LW++ +SK HRHL+KHPV+TSF++LKW +IRR FNRNLRFYLLFV +LTWYIF RFGGKSL    + +D  +            +IL+A   LV + F++ DW  D  +           +  TS +         +W+E    L    VL+G   +L   L+ L  ++++RE FQ+  S KRY++S ENW+E+ +I ++  ILF N       NRH +AI L++SWAELIT+VG+HPKL  YN+YVTMFYKVL +FF+FL W+ FFIIAF  GFYIMLH+D  N D     DY FF+ PW  L KT TMFVGE+EFSDIP++  +       L  ++Y F L+FVF IVVVLMNLLNGLA++DTG+IQ +AEIVS ++RVE++SY ES+LLGDPF+FLS   A       WL  ++PSC+L   L +KS T++  F KITGATGILLF+T L T K    P+V K      RV     DI+A++K+I  +K     +EDR
Sbjct:   37 SC-KDPQVGLWKCLTNGDLTTFSSILAEVAEVCGNNNNNGYSTPSILPSSXWINSKI-YENDRNILQLALELEPPEKGIPFVKKLLDAGANADFFNVETDLALIHVAVQSNNLKKVEALLELEWNKGDISVTTQPEGQNALHLAV--NNPGILRRLLKEPKARINAKDNCGSRTPLQLALLAKNRESTILLIENGANLEEKVVGDGRNLRNLVQSTFPDLSIDK--------INIKKQPI-----HYTLDFLSDVLDK---------RDLTLFKSAIRFLDKSEISAGTVRGGVTLLQRAAIEGLNEFVCVLINNGADANM--YSKENLYTPTILAASRGHYQVLESLLYKGKAIIATWTPDSQETLLHVILRKSLRTGHASDQSIYEKCLNVLLESEDFCTQLKPVINKKDDLGNTCLHFAAKSWPQKVTLKLLELGANIGIRNHFGEVPLSRIQTKTLEEFLDNYCTQTKKDPDDYSDSFEVTFRYDFLAPP------VTEEQLQNW--DREKQTQLELE-----ALPETDSLWYIARSKTHRHLIKHPVLTSFMWLKWQKIRRLFNRNLRFYLLFVVLLTWYIFARFGGKSLNIRGDGEDFCSELRFDESYFGFFYILFALHVLVQIGFVITDWYKDYYN-----------DGFTSCI-------LGSWLEIFMFLVFAIVLYGSLGLLKWVLVLLLGVMVLREGFQMLSSFKRYLLSVENWMEIFLIGILSVILFGNSVVPCKWNRHLAAIALLISWAELITMVGRHPKLARYNIYVTMFYKVLESFFYFLIWFSFFIIAFAFGFYIMLHRD--NGDNVDKGDYEFFDSPWLTLAKTFTMFVGEIEFSDIPVDRSTDESEYIALPVIAYLFLLAFVFFIVVVLMNLLNGLAITDTGIIQARAEIVSNVTRVESVSYAESVLLGDPFDFLSSSSANGDGPFSWLNRHLPSCALLNALYTKSPTVRSAFQKITGATGILLFFTLL-TDKSARFPHVAKGLLGG-RVNAEVRDILAKSKDITRKKL--KKKEDR 1006          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000004760 (supercontig:LSalAtl2s:LSalAtl2s250:514305:525469:1 gene:EMLSAG00000004760 transcript:EMLSAT00000004760 description:"augustus_masked-LSalAtl2s250-processed-gene-5.0")

HSP 1 Score: 451.44 bits (1160), Expect = 1.974e-142
Identity = 348/1052 (33.08%), Postives = 517/1052 (49.14%), Query Frame = 0

Query:   20 GSCVGEPQANALHCLLNRELTVFTDVLNDTDTENRSQSFPLPDDHWINQAFPEE-DDKTLLMLALEKD---LHDFTKVLLQTGASGRL--YNHILGRAPVHVAALSGNLRSLQLL----FSLKGNQPDINATMRSNGRTALHLCVEK----------KHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFH-PNQVRR-KNPPIVQQLSQGTFEALADILEKAANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGS-TLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVR--TDETILHCLLKGGRRNDEHFVQD----------------LLDILGNEAIRSQIQKIINQRDLLE-NTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLD-DCIEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQ-SKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTALDG--LNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPNVR-KSRCDCLRVELMDD------------DIIARTKEIVLQKATPNP----EEDRWQKLEGKL 1008
              C+G+PQ  AL  L+ R    F+D ++D   +        P   + N+   E+ + K LL +AL K       F + L+    + R+   N +L  +P+H  A  G+   L+L+    F    N PD        G T LH+ VE            + +C+  LL+   I+ N +D +   T L+ A         + L++ GAD +          +++  K+P F   + V + K+ P+  +L            E   N    G + ++D    L  +D         NGS TLLQ AC  G    V  +L      +P+  +      PIL+AA+ G+   ++     +   D T +   + +T+LH +    R++   F++                 LL    ++  + QI  IIN R+ L+ +T LHYAT    Q ++R+ L  GAN+G+ N  + T V  ILPQTL+ + D DC+  +G +  EKF++T+ Y FLAPPK +   +   + +   SDP           +R  LPET+ LW+M   SK HR LLKHPV+ SFL+LKW RIR YF  NL                                    LY  F ++L AFI   +   I         K+ED      V         + V  +  +FL  +L               ++L  RE FQ+ VS KRY  + EN +E+++I L   I+F +++  +   +NRH +A+ ++ +W EL+ L+G+HPKL+T    + MF  V+GTF  FL WY   I+AFG+ FY+M+H +G  +         FF     +L+KTSTMFVGELEFSDIP + +    ALSY  F+SF+FLIVVVLMNLLNGLAVSDTGLI+E+AE++ ++SRVE I+YTES+LLGDPF FLS WPA+K L  +P C +C  L K   M+ +  K+TG T  LLFY+ LP+K+ T  PN R KS     R   +DD            D++   K ++L+K          ++++QKLE K+
Sbjct:   48 AGCLGDPQRAALDYLIFRHFRSFSDAISDDKFD--------PSYIYENETTDEDRNGKNLLEIALLKSPGGRDRFVETLILHPNATRVDEINELLETSPLHEVARRGDPELLRLVLEAGFPYDVNIPD------GEGLTPLHVVVESLEEADDAKRTCYLKCMELLLNGEHIEPNAEDLRGNLTPLHYAANIGLQQPIRLLLDRGADPNLEFESGVTISDVIKRKIPEFDLADYVYKIKSRPLKSRL-----------FEYVENGDIRGLESVKDEACELWNSD---------NGSLTLLQMACVKGYDGVVRWLLTVG-GADPNARSGGEPRTPILIAAHHGYYRILRIFRDEEPRSDFTIIEPVSGKTVLHEVF---RQDSAKFIKSNVKSVRGSNYRKCFDILLSDTSSKGFKDQIDSIINYREALDGDTPLHYATMQTDQDLIRAFLRRGANMGIKNKKDRTSVQCILPQTLQTFFDEDCVSWDGIITDEKFKLTFLYDFLAPPKLNNLSLEYFEDQEKNSDPSQM--------DRRPLPETEPLWYMSMASKEHRMLLKHPVIASFLWLKWQRIRSYFYINL-----------------------------------FLYFTFVVLLTAFIFLKYGGGIVP-------KNEDGTPVQVV---------DTVNIILQIFLSGLL---------------LVLFCRELFQMGVSFKRYFFNVENLLELTVIALSITIIFGSNSTPEEILMNRHLAALCVLFTWTELLVLIGRHPKLSTN---IVMFRTVIGTFLTFLLWYSLIIVAFGIAFYLMMHSNGGENK--------FFESVSLSLLKTSTMFVGELEFSDIPFSNN----ALSYLIFVSFIFLIVVVLMNLLNGLAVSDTGLIREEAEVLGWLSRVELITYTESMLLGDPFYFLSNWPAIKLLKKLPHCGVCRILYKFPFMRDLLQKVTGGTKXLLFYSCLPSKRATFFPNKRGKSLFGYKRSLKIDDESRKAPNLYVSTDVLESAKXLILEKLRRREEEEIAKNQFQKLEMKI 972          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000007091 (supercontig:LSalAtl2s:LSalAtl2s3:842630:846195:1 gene:EMLSAG00000007091 transcript:EMLSAT00000007091 description:"maker-LSalAtl2s3-augustus-gene-8.27")

HSP 1 Score: 243.817 bits (621), Expect = 2.980e-68
Identity = 192/612 (31.37%), Postives = 296/612 (48.37%), Query Frame = 0

Query:   18 RTGSCVGEPQANALHCLLNRELTVFTDVLNDTDTENRSQSFPLPDDHWINQAFPEEDDKTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCV---EKKHGE---CVNFLLDQPGIDVNIKDKKN-GQTALYMAVKA-------SHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHP---NQVRRKN-----PPIVQQLSQGTFEALADILEKAA-NRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGS-TLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGGRRNDEHFVQD-------LLDILGN-EAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDD-CIEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLF-----VFILTWYIF--ERFGGKSLRDSE 589
            RT SC G+ Q +AL  LL R     + +LN+                 + + +P+E  K LL +A++    +  +++L +GA+    N +L   P+H+A        L++L  L    P+I     ++G+T LHL +   EK   E   C+  LL +    ++I  + + GQ+ L   V +         + I    +++GAD        +     +A+ +  FH    N + RKN     P    ++ +G  + + DIL KA  + + E  +LL+ F++ L       +     +G  + LQ+AC+LGLV +V  +L++ +  NP+ V  +   P ++LA+       +K L +   + D    +  ET+LH +L+         V D       L++I      I  ++  +IN  D L NT LHYATQ W Q VVRSLL+LG+NIGM N + E PV +IL +TLE +    C+E EG++ +E   +T  Y FLAPP  D                     AEGE      LPET  LWHM Q+  HR LLKHPV+TSFL++KW RI   +N+NL FY L+      F L +YI   +  G  SL + E
Sbjct:   12 RTTSCFGDEQESALDALLRRXHQFLSXLLNNGGVN-------------LEKNYPQEQQKNLLSIAVDNRDIESLRLILASGANVNAINPLLKTVPIHIAIRKSQPEILRILL-LNNADPNIKT---ADGKTPLHLAIKSMEKVDNEGYACIKTLLREGSKKLSIDAEDSLGQSPLSAFVSSLNANPTNRDIEIITLFLKSGADSKAKVVNDS---RTLAEIIENFHDSELNALVRKNKAEFHPLSSNRVQEGILKDVYDILNKADFSNEAEKSRLLKKFKTTLGYLKDEQITASPPSGVLSPLQRACELGLVDFVQVLLDKGH--NPN-VHSSGTTPAVILASKSARSNVLKLLAEHGKT-DFGANKDGETVLHAILRKPYSGSLSEVMDYENAWETLVEIRSEYPTINHELSSVINSTDELLNTPLHYATQCWSQKVVRSLLELGSNIGMKNSYGEIPVDRILSETLEDFFSSYCLEHEGNIANEDLRITVKYDFLAPPVHD--------------------KAEGEE----LLPETDVLWHMSQNSNHRRLLKHPVITSFLWMKWKRISGSYNKNLLFYFLYSMFIIAFGLGFYILLHDDDGSSSLENKE 575          

HSP 2 Score: 209.92 bits (533), Expect = 9.508e-57
Identity = 112/220 (50.91%), Postives = 149/220 (67.73%), Query Frame = 0

Query:  748 KHPKLTTYNVYVTMFYKVLGTF-----FFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPN 962
            KHP +T++      + ++ G++     F+FL  Y  FIIAFGLGFYI+LH D  +  +    +Y FF+     LVKT TMFVGELEFSD+PI+       + Y F +SFVFLIVVV+MNLLNGLAVSDTG+I+E+AEI S + +V+ ISY E++LLGDPFNFLS WP   WL N P C+L +++ K   ++ +F KITG   ILLFY  LP K+++  PN
Sbjct:  518 KHPVITSF--LWMKWKRISGSYNKNLLFYFL--YSMFIIAFGLGFYILLHDDDGSSSLEN-KEYPFFDYLGLTLVKTFTMFVGELEFSDLPIST-----PVGYLFLISFVFLIVVVMMNLLNGLAVSDTGVIREEAEIHSIVXQVDVISYLEAMLLGDPFNFLSNWPTFVWLRNFPDCNLXSRIYKVPALRTLFQKITGGPKILLFYDRLPDKRVSFYPN 727          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000012817 (supercontig:LSalAtl2s:LSalAtl2s977:9979:21258:1 gene:EMLSAG00000012817 transcript:EMLSAT00000012817 description:"maker-LSalAtl2s977-augustus-gene-0.7")

HSP 1 Score: 218.009 bits (554), Expect = 2.523e-58
Identity = 178/583 (30.53%), Postives = 277/583 (47.51%), Query Frame = 0

Query:   22 CVGEPQANALHCLLNRELTVFTDVLNDTDTENRSQSFPLPDDHWINQAFPEEDDKTLLMLALEK-DLHDFT-KVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHG-----------------ECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFE-ALADILEKAANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVR--TDETILHCLLKGGRR-----NDEHFVQDLLDILGNEA-IRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDDCIEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYI 576
            CV +   + +  +   +L +F+D+L D             D   +N+ + E ++ T+L LAL +    +F  +VLL+   +  + + I+   P+H+AA  G    +Q L  +     D+NA +  NG TALHL   K +                  +CV  LL +  I+++ ++   G + LY   +     I + L++ GA Y  V     +  E + D L    P       P   Q  S+ T E  L   L K +   G     +  + +   QT  +  +N      + LQ  CDLG  S V+ +++E    +P+       + PILLAA+ G+   +K   +    +D + V     E ILH ++K   +      + ++ + L  I+ +EA  RS IQ  IN  D L NT LH A Q+  +  VR LL   ANIG+ N  +ETP+  I P+ +E YLDDC++ EG +  E++++ ++YSFL PP  D        LRP P +  +          +  LPE + LW+M Q   HR LL HPV+TSFL +KW R+R YF  NL FYLL+V   T Y+
Sbjct:  304 CVPDHGIDLISAMDEGDLRLFSDILAD-------------DALDVNKEYSEYNNSTVLHLALSQPGKEEFALQVLLRPDLNPNIPHKIVRSFPIHLAAEIGAHEVIQSLIDINA---DVNARIE-NGNTALHLACLKSNANWIKDPILKQEAQCNYIKCVEALLKKENINIDCENNL-GISPLYFGAEKGSQEIIQLLIKKGA-YAKV----EVDDESIEDHLNARFPAGTFSLTPANTQ--SKDTIENKLFSALYKESTSPG---TFIATYNTYKDQTIKWNADN---GAYSFLQYCCDLGFKSLVSFLIDEG--ADPNYYCPNYKMSPILLAAHHGYYKILKIFKELNNDIDFSAVDPFKKENILHKIIKRESKAYVNFEERNYEKCLSLIVDDEASFRSLIQPTINGADHLGNTPLHVAAQLGQEDTVRKLLRCSANIGIKNLRDETPILHISPEVMEEYLDDCLQGEGLITDERYKIVFSYSFLGPPLIDKKSEKASLLRPEPREMPNMV-------PQYNLPEAEPLWYMSQLPGHRALLTHPVITSFLCMKWRRMRHYFYINLLFYLLYVGFFTTYL 846          

HSP 2 Score: 197.593 bits (501), Expect = 8.792e-52
Identity = 122/290 (42.07%), Postives = 169/290 (58.28%), Query Frame = 0

Query:  674 LLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTM-FVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPN 962
            ++ L +LL IREF Q  VS +RYI S EN +E+S++ L  +I F  +   +  +R      L+L+WAE++ L G+HPK +T+   VTMF ++   F  FL W + FIIAF L FYIML K    +   G     +F     +L+KT TM   GE+EF  I  +L           FL +VF I++VL+NLLNGLAVSD   IQ++AEIV+++SR+E ISY ES+LLGDPF+F S WP   W   +P CS    + +SR M ++     G+   LLF   L  K+    PN
Sbjct:  867 VMVLLVLLTIREFLQAFVSFRRYIFSVENILEISLVSLSFYICFTENLKTES-SRSICVAALLLTWAEMVLLFGRHPKASTF---VTMFKRISQNFILFLTWSISFIIAFALAFYIMLKKPATKE---GEQPNPYFTTVERSLLKTITMSLTGEIEFESIEFDL-----PFGRIVFLLYVFFIMLVLVNLLNGLAVSDISSIQKEAEIVNHVSRIELISYFESMLLGDPFHFFSNWPPFSWGRKVPDCSCLRGVFQSRLMSKLIGLTLGSKRFLLFSERLAEKQAIFYPN 1144          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000005636 (supercontig:LSalAtl2s:LSalAtl2s3031:10533:12187:1 gene:EMLSAG00000005636 transcript:EMLSAT00000005636 description:"maker-LSalAtl2s3031-augustus-gene-0.2")

HSP 1 Score: 122.865 bits (307), Expect = 1.074e-29
Identity = 132/503 (26.24%), Postives = 210/503 (41.75%), Query Frame = 0

Query:  427 LLDLGANIGMTNHWNETPVTKILPQT------LEAYLDDCIE-AEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERF--GGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSED-------EEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWND--------TALDGL-NRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRP----WTALVKTSTMFVGELEFSDIPINLDSRLGALS-YAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLG 899
            LL  GA+I        + ++ I+ +T       E  LD  I  AE D+N    E+  ++  L P +                         GE++  +   ET           H HLLKHP+  SFL+LKW ++R++F  +L F+LLF  + T ++ + +  G   +RD+                             D + + R+   +S +       EE        L V    W+  LF                      T +L+ +E FQ++ S K Y  + ENW+++ +I+ +  I F  D          L G+   H +AI + L W EL+ ++G+ P   T+ +YV MF  V   F  FL  Y   ++AF L F ++     PN        Y  FN       +A++KT  M  GE+E+ +        L   + +   L FV L+ ++LMNLL GLAVSD   +Q+ A +   + + E IS+ ES+L  
Sbjct:    2 LLAFGADITARTKGGTSALSMIVRKTPNVLPKFEDMLDHAITLAEHDINDVDCELKLDFRVLIPNR-----------------------TRGESNMFINFIET----------GHNHLLKHPLCESFLHLKWLKVRKFFLVSLIFHLLFTILHTTFVLQVYYSGQCIVRDNCKYGNET----------------------DFQKIERTPYWESVNGDCFDPFEEQCKIXSLTLFV----WISLLFS---------------------TSILMGKECFQLAHSQKMYFYNWENWVQLGIILDVILISFHKDPFDSLEHYIPLIGIWQHHAAAIGVFLVWGELMLMIGRLP---TFGIYVQMFTTVAKNFAKFLAAYFCLLVAFALSFCVLF----PN--------YQSFNVKGRGILSAVIKTLVMMAGEIEYENFIYENGQNLYVFTGHLMVLIFVLLVSIILMNLLVGLAVSDIQGLQKSAGLDRLVRQTELISHIESMLFS 409          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000012234 (supercontig:LSalAtl2s:LSalAtl2s88:714578:730796:-1 gene:EMLSAG00000012234 transcript:EMLSAT00000012234 description:"maker-LSalAtl2s88-augustus-gene-6.25")

HSP 1 Score: 103.219 bits (256), Expect = 2.201e-22
Identity = 177/808 (21.91%), Postives = 322/808 (39.85%), Query Frame = 0

Query:  109 GRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTA---------LYMAVKASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKA--------ANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGGRRNDEHFVQDLLDILG-NEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDDCIEAE-GDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSE-DEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTAL---DGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDI--PINL-DSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETI 890
            G  P+H+A   GN+ + ++L + K N       +  + R+ L++       E +  LL+         DK NG  +         L++A K  ++ I   L+ AG+D D          +   D++   H   +  +   +V+++ +    A++DI E +         NR+    K L ++ +         + +R     T L  A  +G    V  +L    +++P    +     P+ LA+  GH   ++ L+ +  SL + +      +   LLK  +      V+ L++      A R+ +  +   R  +  T L    + +P  V   L      +       E  V        +AYL   +E E  +V+   ++V + Y  +    +  PF+S                       R  + +      M + K +R LL+HP+  + +  KW    R F R              Y+F  +G                 LY+ F + L +++L    +++K    S+   S+ D +   +       MF  +  C   + +G +L               IL  I E  +  +   RY  S +N ++  + IL  +I+  +   +   D      +AI++ L+W  L   + + P L    +YV MF+ +L TF  F   ++ F+IAFGLGF+ +     P            F  P  + +KT+ M +GE E+ DI  P N  D + G +S  FF  FV ++ +++MNLL GLAV D   +Q++A +   + + E +
Sbjct: 1774 GFTPLHIACKEGNVEAXEVLIAFKTNVD----LLDYDDRSCLYIAALYNKPEVLKALLEN--------DKCNGLISVNDLFDNLPLHVASKEGNVEIVHLLINAGSDID----------KKNEDEMTALHLASLNGR-VKVVEEILKSDRCAISDIDEDSNTALHIACTNRRFLVSKKLLEYGA--------DIQSRNVKKWTPLDCAASVGAYKCVELLLESGAQIDP---LDRSKTTPLHLASEFGHEKTVRLLLSNGASLKMEDAFGRNALEIALLKKNKS----VVKALIEYPDWKNAFRTCLTPLHKLRHKVPETPLRLLIKTYPDLVETVLDSCITTVENPEKCGEKKVIMNYEFLDDAYLWQPVEKEVKNVHGITYDVVFEYCQVDENAQCEPFLSTYH------------------KNRRIVNQNHPFMIMAEEK-NRDLLRHPLCLALVRHKW----RKFGR--------------YVF--YGR--------------VFLYSLFLVFLTSYLL----LEVK---HSQCFGSDIDPKTDPNELLPPQNMFKTF--CHVGVLIGVIL--------------NILAEITEIIRTGI---RYF-SFDNLMDWVVCILSLYIISVDTIFVPEKDCSRWDVAAILITLAWLNLXAXIXQLPYL---GIYVIMFFDILLTFIKFALIFMVFVIAFGLGFHFLHTSHDP------------FQTPGDSFLKTTVMMIGEYEYVDIFFPGNENDPKPGGMSKVFFGIFVVVMSLIVMNLLVGLAVEDIKAVQDQAVLKRLVMQAELV 2448          

HSP 2 Score: 53.9138 bits (128), Expect = 2.475e-7
Identity = 31/90 (34.44%), Postives = 45/90 (50.00%), Query Frame = 0

Query:  117 ALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGAD 206
            ALSG+L  L+         PD+   + S+G TALH      H   V+FL+++ G  VNI  +  G T L+ A    H+ I   L++ G D
Sbjct:   67 ALSGDLNKLRERIL---RNPDLTNALDSSGLTALHYASRSGHFNIVSFLVNEGGAKVNIASRSGGATPLHRAAYMGHIQIVSFLIDHGGD 153          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000005243 (supercontig:LSalAtl2s:LSalAtl2s279:373559:377813:-1 gene:EMLSAG00000005243 transcript:EMLSAT00000005243 description:"maker-LSalAtl2s279-snap-gene-3.20")

HSP 1 Score: 72.0182 bits (175), Expect = 1.233e-13
Identity = 64/197 (32.49%), Postives = 94/197 (47.72%), Query Frame = 0

Query:  699 SPENWIEVSMIILIGFILFWND-----TAL-DGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGA-----------LSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKA 878
            S EN +E +  IL   +++  D     T L  G      A+++ LSW  L++ V K P L    +YV MF  VL TF  F      FI+AF LGF  +L          G ++   FN    +++KT  M +GE E+ DI  +     G            L+  FFL F+ ++ +++MNLL GLAV D   +Q+ A
Sbjct:  111 SFENLVEWTCYILASILVYDFDECQQITGLRSGWQWQIGAVIVTLSWLNLLSNVRKVPLL---GIYVVMFSDVLKTFLKFSIICGLFIVAFSLGFQTLL---------GGQEN---FNTIDKSMMKTIVMMIGEFEYDDIFYDNVRNAGETLKRSSLPYSYLTQGFFLVFLIIMPIIIMNLLVGLAVDDIKAVQDNA 292          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000008562 (supercontig:LSalAtl2s:LSalAtl2s527:33181:43889:-1 gene:EMLSAG00000008562 transcript:EMLSAT00000008562 description:"maker-LSalAtl2s527-snap-gene-0.21")

HSP 1 Score: 60.8474 bits (146), Expect = 1.561e-9
Identity = 64/251 (25.50%), Postives = 104/251 (41.43%), Query Frame = 0

Query:  671 WHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWND-----TALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDT---GLIQEK-----AEIVSYISRVETISYTESILLGDPFNFLSQW 908
            W  L+   IL +++E  +      RY+   E+ + V +II+   I F +D       L     H +   +  +W  L+ L+GK P+   +  YV MF  V  TF  FL  YVF  +AF L FY+++           P    F      A VK   M +GE+E+ D+      R+                      + G   + +   GLI+ +     A++   I +V+ ISY ES++L   F +   +
Sbjct:  542 WIFLVIFNILYILKETTKCIHLGYRYLREYESLLNVFIIIIFLLISFHSDPFSETIVLSAWQYHAAGFGVFSTWLFLMFLIGKVPR---FGKYVEMFKTVSETFLDFLIAYVFLFVAFALTFYLLI-----------PSHVAFNGSLPAAFVKVLVMMLGEIEYDDLYYPEKHRIK---------------------MTGPTTNKSGWEGLIESEELYKTAKLHQRIQQVQLISYMESVILSPLFQYFPDF 757          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|56749363|sp|Q9W0Y6.1|PAIN_DROME (RecName: Full=Transient receptor potential cation channel protein painless)

HSP 1 Score: 135.191 bits (339), Expect = 5.499e-31
Identity = 129/516 (25.00%), Postives = 200/516 (38.76%), Query Frame = 0

Query:  401 INQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDDCIEAEGDV-NHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTALDG-LNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVA------------GPDDYVFFN---RPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLG 899
            IN+ D      L +A +    + ++ LL  GA IG  + +   P+  + P+ LE + D CI   G+    + FE+  +Y  L   + D      QD                         E   +  + +SK  RHLL+HP+++SFL+LKW R+   F  N                                   F++Y+ F   ++ + L              K    D+ A ++                   F G + W G              L++RE  Q  +S  RY  S  N +EV++I L  F     +++ D    R  +   ++L   E   LVG  P L+  + ++ M  +V  +F      Y  F++ F L FYI+  K    D               G D+   FN   +P  A++KT  M  GE +   I           +Y  FL FV  + +VL NLLNGLAVSDT +I+ +AE+   I R   +S  E +L G
Sbjct:  362 INEADSGRLVPLFFAVKYRNTSAMQKLLKNGAYIGSKSAFGTLPIKDMPPEVLEEHFDSCITTNGERPGDQNFEIIIDYKNLMRQERDSGLNQLQD-------------------------EMAPIAFIAESKEMRHLLQHPLISSFLFLKWHRLSVIFYLN-----------------------------------FLIYSLFTASIITYTLL-------------KFHESDQRALTA-------------------FFGLLSWLG-----------ISYLILRECIQWIMSPVRYFWSITNIMEVALITLSIFTCM--ESSFDKETQRVLAVFTILLVSMEFCLLVGSLPVLSI-STHMLMLREVSNSFLKSFTLYSIFVLTFSLCFYILFGKSVEEDQSKSATPCPPLGKKEGKDEEQGFNTFTKPIEAVIKTIVMLTGEFDAGSIQFT-----SIYTYLIFLLFVIFMTIVLFNLLNGLAVSDTQVIKAQAELNGAICRTNVLSRYEQVLTG 766          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|62900887|sp|Q9W0T5.2|PYX_DROME (RecName: Full=Transient receptor potential channel pyrexia)

HSP 1 Score: 107.842 bits (268), Expect = 1.688e-22
Identity = 77/224 (34.38%), Postives = 115/224 (51.34%), Query Frame = 0

Query:  684 REFFQVSVSLKRYIVSPENWIE------VSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDY-VFFNRPWTALVKTSTMFVGELEFSDIPI-NLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLG 899
            +E FQ++  L+ Y    ENW++      V + +    +   + TA+     H +AIV++L W EL+ LVG+ P    + VYV MF KV   F  FL  Y+  ++AFGL F ++             +DY  F N  W+ L K+ TM  GELEF DI   +   +    ++  FLSFV L+ V+L NL+ GLAVSD   +Q  A +   + + E +S  ES+   
Sbjct:  548 KEVFQMAHGLRGYAKYWENWLQWTIGTGVLLCVTPETVRTDDLTAVPVWQHHVAAIVILLVWLELMMLVGRFP---IFGVYVQMFTKVAVNFAKFLLAYICLLVAFGLSFAVLF------------NDYPAFENITWSFL-KSITMMSGELEFEDIFYGDYAVKFPVTAHIIFLSFVLLVTVILTNLMVGLAVSDIQGLQVSATLDRLVRQAELVSRLESLFFS 755          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|657193258|sp|Q7Z020.4|TRPA1_DROME (RecName: Full=Transient receptor potential cation channel subfamily A member 1; Short=dTRPA1; AltName: Full=Ankyrin-like with transmembrane domains protein 1; Short=dANKTM1)

HSP 1 Score: 101.293 bits (251), Expect = 2.179e-20
Identity = 193/844 (22.87%), Postives = 344/844 (40.76%), Query Frame = 0

Query:   92 KVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGR-TALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKAS----HLVITKAL---VEAGADYDYVCF-----GK-TIFPELMAD-----KLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKAA-----NRKGEGP-------KLLEDFRSLL-LQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTD-ETILHCLLKGGRRNDEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQM-WPQTVVRSLL-DLGANIGMTNHWNETPVTKI-----LPQTLEAYLDDCI-EAEGDVNHEKFEVTYNYSFLAPPKEDLPFV-SRQDLRPSPSDPESQKLAEG-ETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFN-RNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSED--EEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIE----VSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHK-DGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSR----LGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEI 880
            ++ L++GA      H L   PVH+A   G +  ++L+F ++  +  +  +     + T LH      H + V++L+ + G D+N  DK++    L  A ++     HL+I       V+  A  + + F     G+ T F E +A+     +L      +      P+      G   +L +++   A     N   E P             R LL  +  SF +N     G T L  +   G    V  +LN    ++ D         P+ LAA  G+   I EL+ S  S  L  V  D  T LH        N  H +  L+  +G + +           ++L+ +A+ YA    +P+  +  +  +  AN  M    ++ P   +     +P+  EA  D CI +A    + + F + Y+++FL       PF+ ++ D          +K  E   T   + LP   T+   G+ +    LL HP+   +L +KW+   +YF+  NL  Y   +F++   I+      ++             L A     +  +    W     +L ++  V S+   +     +     ++F   V  +++L                      L  +RE  Q+      YI+   N I     +S ++++    F  D  ++ ++   ++I + LSW  L+  + +  ++    +YV MF ++L T    L  +   IIAFGL FYI+L K   P      P+   F N P + L++T +M +GEL+F    +N   R    +   S+     F+ L+ ++LMNLL GLAV D   ++  A++
Sbjct:  283 ELCLKSGAKISTQQHDLS-TPVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAE-GADINALDKEHRSPLLLAASRSGWKTVHLLIRLGACISVKDAAARNVLHFVIMNGGRLTDFAEQVANCQTQAQLKLLLNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNESPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGALLHRDHTGRN----PLQLAAMSGYTETI-ELLHSVHSHLLDQVDKDGNTALHL---ATMENKPHAISVLMS-MGCKLVY----------NVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFAFL-----QCPFMFAKID----------EKTGESITTASPIPLPALNTMVTHGRVE----LLAHPLSQKYLQMKWNSYGKYFHLANLLIY--SIFLVFVTIYSSLMMNNIE------------LKAGDNKTMSQYCNMGWEQLTMNLSQNPSVASQIRLDSCEERINRTTAILFCAVVIVVYIL----------------------LNSMRELIQIYQQKLHYILETVNLISWVLYISALVMVT-PAFQPDGGINTIHYSAASIAVFLSWFRLLLFLQRFDQV---GIYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKIIDPQ-----PNHLSFSNIPMS-LLRTFSMMLGELDFVGTYVNTYYRDQLKVPMTSFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQL 1040          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|205831270|sp|Q18297.5|TRPA1_CAEEL (RecName: Full=Transient receptor potential cation channel subfamily A member 1 homolog)

HSP 1 Score: 96.6709 bits (239), Expect = 5.116e-19
Identity = 71/245 (28.98%), Postives = 122/245 (49.80%), Query Frame = 0

Query:  669 ILWHALL-FLTILLLIREFFQVSVSLKRYIVSPENWIE--VSMIILIGFILFWNDTALDGLNRHF----SAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFS-----DIPINLDSRLG----ALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESIL 897
            ++W  ++  L +  ++ E FQ+      Y+V+ ENWI+  +    LI    F   +A  G+ +++    +A+ +   W  L+ ++ K P+   + ++V MF  ++ TFF F   +V FIIAF   FY++L           P+    F  P    +KT+ M +GE EF+     D   + +   G    A++ A F  F  ++ ++LMNLL GLAV D   +QEKAE+     +V+ +   E+ L
Sbjct:  869 VVWKIIIQTLAVCQILVECFQLFQRKFAYLVNWENWIDCFIYSTALITVYDFSECSATSGVRQNWQWILAALCIFFGWINLLFMIRKMPR---FGIFVVMFVDIVKTFFRFFPVFVLFIIAFSSSFYVILQN--------RPEFSTIFMSP----LKTTVMMIGEFEFTGIFHGDETTHAEKMFGPAHTAVACALFFFFCIIMTILLMNLLVGLAVDDIKGVQEKAELKRLAMQVDLVLQIEASL 1098          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|313104269|sp|O75762.3|TRPA1_HUMAN (RecName: Full=Transient receptor potential cation channel subfamily A member 1; AltName: Full=Ankyrin-like with transmembrane domains protein 1; AltName: Full=Transformation-sensitive protein p120)

HSP 1 Score: 90.1225 bits (222), Expect = 6.229e-17
Identity = 184/880 (20.91%), Postives = 321/880 (36.48%), Query Frame = 0

Query:   70 FPEEDDKTLLMLALEKDLHDFTKVLLQTGAS------GRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYV-CFGKTIFPELMADKLPGFHPNQVRRKNPPIVQ--QLSQGTFEALADILE--KAANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGGRRNDEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGM-------------------------TNHW-------------NETPVTKI---LPQTLEAYLDDC-IEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKW-SRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPEN---WIEVSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSD--IPINLDSRLG--ALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVE 888
            F      T L LA++    +  K+ L  GA       GR          +H AA  G    ++L+ S      DI  T      T LH      H E  ++L+   G D+N  D + G++ L +A  ++   I   L+  GA  D    FG+  F  L   +     P  ++   P  +Q  Q+ +   +   D       A R+G GP  + +         + +++++  +  + L  A   G ++    +L +  +       +   + P+ LAA  GH   ++ L++ K +L L++     T LH    GG      + Q +  IL          K  ++ D   NTALH+A +      V  LL   A+I +                         +  W             N+ P+T++   LP+ ++  LD C + +  D +   + + YN+ +L  P E       QD+   P    +  +     +                      LL HPV   +L +KW +   R    NL  Y L +  +T                                +LV  I    A +   ++      SE  + T+S              C+ ++FL ++                     +E  Q+    + Y +   N   WI  +  I+    LF    A   L     AI +   W   +  +    +     +++ M   +L T       ++F ++AFGL FYI+L+   P            F+ P  ++++T +M +G++ + +  +   L + L    LS+A  +SF   + +VLMNLL GLAV D   +Q+ A +     +VE
Sbjct:  234 FMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRC-------TAIHFAATQGATEIVKLMISSYSGSVDIVNTTDGCHETMLHRASLFDHHELADYLIS-VGADINKIDSE-GRSPLILATASASWNIVNLLLSKGAQVDIKDNFGRN-FLHLTVQQ-----PYGLKNLRPEFMQMQQIKELVMDEDNDGCTPLHYACRQG-GPGSVNNLLGF-----NVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLK-KGALFLSD-HNGWTALHHASMGG------YTQTMKVILDTNL------KCTDRLDEDGNTALHFAAREGHAKAVALLLSHNADIVLNKQQASFLHLALHNKRKEVVLTIIRSKRWDECLKIFSHNSPGNKCPITEMIEYLPECMKVLLDFCMLHSTEDKSCRDYYIEYNFKYLQCPLEFTKKTPTQDVIYEPLTALNAMVQNNRIE----------------------LLNHPVCKEYLLMKWLAYGFRAHMMNLGSYCLGLIPMT--------------------------------ILVVNIKPGMAFNSTGIINETSDHSEILDTTNSYLIK---------TCMILVFLSSIFG-----------------YCKEAGQIFQQKRNYFMDISNVLEWIIYTTGIIFVLPLFVEIPA--HLQWQCGAIAVYFYWMNFLLYL---QRFENCGIFIVMLEVILKTLLRSTVVFIFLLLAFGLSFYILLNLQDP------------FSSPLLSIIQTFSMMLGDINYRESFLEPYLRNELAHPVLSFAQLVSFTIFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVE 981          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|56749669|sp|Q6RI86.1|TRPA1_RAT (RecName: Full=Transient receptor potential cation channel subfamily A member 1; AltName: Full=Ankyrin-like with transmembrane domains protein 1)

HSP 1 Score: 83.1889 bits (204), Expect = 7.137e-15
Identity = 178/895 (19.89%), Postives = 331/895 (36.98%), Query Frame = 0

Query:   70 FPEEDDKTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKA------ANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGGRRNDEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGM-------------------------TNHWNET-------------PVTKI---LPQTLEAYLDDC-IEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIR-RYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPEN---WIEVSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLH-KDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPIN--LDSRLG--ALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQ 907
            F      + L LA++    D  K+ L +GA   +  +    A +H AA  G    ++L+ S      DI   +  N  T LH      H +  ++L+   G D+N  D + G++ L +A  ++   I   L+  GA  D        F  L   +     P  +R   P  +Q   Q   E + D           A R+G  P  + +         + +++++  +  + L  A   G ++    +L +  +       +   + P+ LAA  GH   ++ L++ K +L L++     T LH    GG      + Q +  IL          K  ++ D   NTALH+A +      V  LL   A+I +                         +  W+E              P+ ++   LP+ ++  LD C I +  D + + + + YN+ +L  P                       + +  T  +  + E  T+ ++        LL HPV   +L +KW     R    NL  Y L +  +T                                +LV  I    A +   ++ +E + + +E   +   F L +       C+ ++FL ++                     +E  Q+    + Y +   N   W+  +  ++    LF +  A   +     AI +   W   +  +    +     +++ M   +  T       ++F ++AFGL FY++L+ +D              F+ P  +L++T +M +G++ + D  +     + L    L++   ++F   + +VLMNLL GLAV D   +Q+ A +     +VE  +  E  L   PF +L +
Sbjct:  235 FVNHKKASPLHLAVQSGDLDMIKMCLDSGAHIDMMENAKCMA-LHFAATQGATDIVKLMISSYTGSSDIVNAVDGNQETLLHRASLFDHHDLADYLIS-VGADINSTDSE-GRSPLILATASASWNIVNLLLSKGAKVDIKDHLGRNFLHLTVQQ-----PYGLRNLRPEFLQM--QHIKELVMDEDNDGCTPLHYACRQG-APVSVNNLLRF-----NVSVHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLK-KGALFLSD-HNGWTALHHASMGG------YTQTMKVILDTNL------KCTDRLDEEGNTALHFAAREGHAKAVAMLLSYNADILLNKKQASFLHIALHNKRKEVVLTTIRSKRWDECLQVFTHDSPSNRCPIMEMVEYLPECMKVLLDFCMIPSTEDKSCQDYHIEYNFKYLQCP---------------------LSMTKKVTPTQDVIYEPLTILNVMVQHNRIELLNHPVCREYLLMKWCAYGFRAHMMNLGSYCLGLIPMT--------------------------------LLVVKIQPGMAFNSTGII-NETISTHEERINTLNSFPLKI-------CMILVFLSSIFG-----------------YCKEVVQIFQQKRNYFLDYNNALEWVIYTTSMIFVLPLFLDIPAY--MQWQCGAIAIFFYWMNFLLYL---QRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDA-------------FSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQLIAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTNLEKKL---PFWYLRK 1000          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|75289839|sp|Q6AWW5.1|Y5262_ARATH (RecName: Full=Ankyrin repeat-containing protein At5g02620)

HSP 1 Score: 69.707 bits (169), Expect = 6.495e-11
Identity = 48/137 (35.04%), Postives = 72/137 (52.55%), Query Frame = 0

Query:   73 EDDKTLLMLALEKDLHDFTKVLLQTG----ASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGA 205
            +  +T L +A E    D  K+L++      A  +  N   G    H+AA +GNL+ L +L       P+++ T  S+  TALH    + HGE V FLLD+ G+D+    + NG+TAL+ A +  H VI K L+E  A
Sbjct:   54 QSGETALYVAAEYGYTDMVKILMKHSDSVLAGTKAKN---GFDAFHIAAKNGNLQVLDVLIEA---NPELSFTFDSSKTTALHTAASQGHGEIVCFLLDK-GVDLAAIARSNGKTALHSAARNGHTVIVKKLIEKKA 183          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|81908906|sp|Q505D1.1|ANR28_MOUSE (RecName: Full=Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A; Short=PP6-ARS-A; Short=Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A; AltName: Full=Ankyrin repeat domain-containing protein 28; AltName: Full=Phosphatase interactor targeting protein hnRNP K; Short=PITK)

HSP 1 Score: 67.3958 bits (163), Expect = 5.836e-10
Identity = 120/457 (26.26%), Postives = 180/457 (39.39%), Query Frame = 0

Query:   40 TVFTDVLNDTDTENRSQSFPLPDDHWINQAFP------------EEDDKTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINAT--MRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCF-----GKTIFPELMADKLPG-----FHPNQVRRKNPPIVQQLS---------QGTFEALADILEKAANRKGEGPKLL----------EDFRSLLLQTDSFTLNNRV-TNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLT-NVRTDETILHCLLKGGRRNDEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQ 451
            T  TD+L+D+D         L   H  +QA                  +T L LA  K   +   VL+  GAS  + +++L R P+H AA +G+   L+LL    GN    NA      NG+T L L V   H +CV  LL++ G +V+ KDK  G+TAL+      H     AL++ GA     C      G+T      A    G              NP +V              G    +  +LE+   +K +G              E    +L+ +   ++ N   + G T L  A     V  +  +L++  +VN     ++    P+++AA  G    ++ LV S  S DLT   ++  T LH     G     H    LL IL  E I    + +IN  +    T LH A +     VV+ LL  GA++   +    TP     P 
Sbjct:  536 TSGTDMLSDSDNRATISPLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYVLKRTPIHAAATNGHSECLRLLI---GNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNK-GANVDAKDKW-GRTALHRGAVTGHEECVDALLQHGAK----CLLRDSRGRTPIHLSAACGHIGVLGALLQSATSVDANPAVVDNHGYTALHWACYNGHETCVELLLEQDVFQKIDGNAFSPLHCAVINDNEGAAEMLIDSLGASIVNATDSKGRTPLHAAAFTDHVECLQLLLSQNAQVNS---ADSTGKTPLMMAAENGQTNTVEMLVSS-ASADLTLQDKSKNTALHLACGKG-----HETSALL-IL--EKITD--RNLINATNAALQTPLHVAARNGLTMVVQELLGKGASVLAVDENGYTPALACAPN 969          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|332278249|sp|O15084.5|ANR28_HUMAN (RecName: Full=Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A; Short=PP6-ARS-A; Short=Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A; AltName: Full=Ankyrin repeat domain-containing protein 28; AltName: Full=Phosphatase interactor targeting protein hnRNP K; Short=PITK)

HSP 1 Score: 66.2402 bits (160), Expect = 1.040e-9
Identity = 118/454 (25.99%), Postives = 174/454 (38.33%), Query Frame = 0

Query:   40 TVFTDVLNDTDTENRSQSFPLPDDHWINQAFP------------EEDDKTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINAT--MRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGAD-------------YDYVCFGKTIFPELMADKLPGFHPNQVRRKNPPIVQ---QLSQGTFEALADILEKAANRKGEGPKLL----------EDFRSLLLQTDSFTLNNRV-TNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLT-NVRTDETILHCLLKGGRRNDEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQ 451
            T  TD+L+D+D         L   H  +QA                  +T L LA  K   +   VL+  GAS  + ++IL R P+H AA +G+   L+LL    GN    NA      NG+T L L V   H +CV  LL++ G +V+ KDK  G+TAL+      H     AL++ GA                  C    +   L+         N     N            G    +  +LE+   +K EG              E    +L+ T   ++ N   + G T L  A     V  +  +L+   +VN     ++    P+++AA  G    ++ LV S  S +LT    +  T LH     G     H    LL IL  E I    + +IN  +    T LH A +     VV+ LL  GA++   +    TP     P 
Sbjct:  536 TSGTDMLSDSDNRATISPLHLAAYHGHHQALEVLVQSLLDLDVRNSSGRTPLDLAAFKGHVECVDVLINQGASILVKDYILKRTPIHAAATNGHSECLRLLI---GNAEPQNAVDIQDGNGQTPLMLSVLNGHTDCVYSLLNK-GANVDAKDKW-GRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALL-QSAASMDANPATADNHGYTALHWACYNGHETCVELLLEQEVFQKTEGNAFSPLHCAVINDNEGAAEMLIDTLGASIVNATDSKGRTPLHAAAFTDHVECLQLLLSHNAQVNS---VDSTGKTPLMMAAENGQTNTVEMLVSS-ASAELTLQDNSKNTALHLACSKG-----HETSALL-IL--EKITD--RNLINATNAALQTPLHVAARNGLTMVVQELLGKGASVLAVDENGYTPALACAPN 969          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|82194904|sp|Q5F478.1|ANR44_CHICK (RecName: Full=Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B; Short=PP6-ARS-B; Short=Serine/threonine-protein phosphatase 6 regulatory subunit ARS-B; AltName: Full=Ankyrin repeat domain-containing protein 44)

HSP 1 Score: 66.2402 bits (160), Expect = 1.175e-9
Identity = 112/423 (26.48%), Postives = 167/423 (39.48%), Query Frame = 0

Query:  109 GRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYV-CFGKTIF-------PELMADKLP--GFHPNQVRRKNPPIVQQLSQGTFEALADILEKAANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGSTLLQKACDLGLVS-------YVAEILNEEYEVNP---DGVTEARA----IPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGGRRNDEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDDCIEAEGDVNHEKFEVTYNYSFLAPPKED---LPFVSRQDLRPSPSDPE 504
            GR  +H AAL+G++  + LL +   N   INA  + + R ALH      H E V  L++  G +V  KDKK G T L+ A     + I K L+  G + D +  +G T          + + ++L   G + NQ        +   +  T  AL   LE   N   +     +D +S L  T    ++ R T   TL+Q   ++  V        +VA     E  +N     G   A+     + P+ LAA   H  C ++L+ S   +D T      T LH    GG          LL   G +          N++D    T LHYA        + +L+  GANI  T+ W  TP+       ++   +  I      N E+ E T         KE    L F+ + D  PS  D E
Sbjct:  140 GRTALHHAALNGHVEMVNLLLAKGAN---INAFDKKD-RRALHWAAYMGHLEVVALLINH-GAEVTCKDKK-GYTPLHAAASNGQINIVKHLLNLGVEIDEMNIYGNTALHIACYNGQDSVVNELIDYGANVNQPNNNGFTPLHFAAASTHGAL--CLELLVNNGADVNIQSKDGKSPLHMT---AVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARYGHELLINTLITSGADTAKCGIHNMFPLHLAALNAHSDCCRKLLSSGFEID-TPDSFGRTCLHAAAAGGNVE----CIKLLQSSGAD---------FNKKDKRGRTPLHYAAANCHFHCIETLVTTGANINETDDWGRTPLHYAAASDMDRKKN--ILGNSHENAEELERTSEMK----EKEAALCLEFLLQNDANPSIQDKE 531          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325318857|ref|XP_023341191.1| (transient receptor potential cation channel subfamily A member 1-like [Eurytemora affinis])

HSP 1 Score: 865.529 bits (2235), Expect = 0.000e+0
Identity = 464/956 (48.54%), Postives = 625/956 (65.38%), Query Frame = 0

Query:   16 FVRTGSCVGEPQANALHCLLNRELTVFTDVLNDTDTENRSQSFPLPDDHWINQAFPEEDDKTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFS--LKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKAANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGGRRNDEHF----VQDLLDIL---GNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDD-CIEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTA-LD----------------------GLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNK 938
             +R  SCVG+P + AL  L +R+L  FTD+LN T+ +  S+S PLP++HWIN    EE+ +TLL  A+   LH F +VLL  GA   LYNH L   P+HVAA  G  + ++LL +   K N+ D+NA  +  GRTALH+ VE+   + V++LL  PG+ VN KDKK GQT LY+AV      + + L+E GA+   +CFGK I  +L+++K+P    ++V+    P+ ++ S  T + L  +++KA  +       L+ F+SLLLQ D  TL++  T G +LLQK C  GL  +V  +L E   ++P+         P+LLA  RGH+  ++ L +      +   +TDETILH +L      DEHF     Q  LD+L    +  ++ QI +IIN++D+  NTALHYATQ W Q +VRSLL+ GANIG+ N W E P+ KI P+T+EA+L++ C+++  DVNHE FE+ + Y+FLAPP E+LP    + L P   D E+ K+++ +  +R  LPET++LW+MGQ+K HRHLLKHPV+TSFL+ KW+RIRRYFNRNLRFYLLFV ILTWY+FE FGG SLR SE   I A + +++ F+  ++ FI++DW  D+KDLV  E +  E     S  K    ++FSNW+E +++  L  +LW G   L+  LL L   L +RE FQV+VS++RYI+  ENWIEV MII I  +L   D   LD                       L RH +A+ L+ SW+ELIT + KHP+L  YNVYVTMFYKVL TFFFFL WY+FFI+AF L FYIMLH+D    + +   +YVFF+ PW +LVKTSTMF+GELEFSDIP+NLDS++  LSY FFLSFVFLIVVVLMNLLNGLAVSDTG IQE +EIVSY+SRV+TISY ES+LLGDPF+F S WP L+  L IPS S C Q  + RT++R   +
Sbjct:   17 LLRATSCVGDPSSTALQSLRSRDLRTFTDILN-TEEDVGSESSPLPEEHWINTPIQEENGETLLSSAISNHLHHFVQVLLAAGAQANLYNHQLANYPIHVAARVGERKCIELLLNSGCKTNRGDVNAVNKV-GRTALHVAVERNDVDLVDYLLQVPGVRVNPKDKKGGQTPLYLAVLNKSPQLVEMLLEQGANLQDICFGKCI-QDLISEKMPDLDISRVKLNRAPLERENSSNTTQILLGLVDKAGAQSKPNQADLQTFKSLLLQFDMKTLSSIKTGGISLLQKCCVNGLDEFVEALLEEG--LDPNYCPPESYSAPVLLAGGRGHLQVLQVLSKYNADFSVLKKQTDETILHRVLI----KDEHFENGDYQGCLDLLLSVNDPDLKDQIDRIINKKDIFGNTALHYATQKWDQNIVRSLLERGANIGIKNRWGEIPIQKIDPETMEAFLNEYCLQSTNDVNHEDFELVFKYNFLAPPLENLP---TEVLGPYSDDEENHKISDRDIQKRYVLPETESLWYMGQTKEHRHLLKHPVITSFLWCKWNRIRRYFNRNLRFYLLFVTILTWYVFETFGGDSLR-SEDGSIKAWYYIFSVFSAAMLIFIIKDWISDVKDLVYQESIL-EGSVRISGSKSYWSLIFSNWLEVVYLAGLAVILWLGPTCLYIVLLVLLCGLALREVFQVAVSIRRYILDLENWIEVGMIICISIVLLRPDVVQLDTGETNNGTSAVQYSENQFEQERDLKRHLAALSLLFSWSELITFIAKHPRLNIYNVYVTMFYKVLSTFFFFLLWYIFFILAFALSFYIMLHRDF---ETSPESEYVFFDSPWLSLVKTSTMFIGELEFSDIPVNLDSKMVPLSYLFFLSFVFLIVVVLMNLLNGLAVSDTGAIQEHSEIVSYLSRVDTISYAESLLLGDPFDFFSSWPRLR--LRIPSLSFCRQFYRHRTIRRFIQR 953          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325320375|ref|XP_023341985.1| (transient receptor potential channel pyrexia-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 759.214 bits (1959), Expect = 0.000e+0
Identity = 448/1102 (40.65%), Postives = 646/1102 (58.62%), Query Frame = 0

Query:    6 SQKKKPTVGDFVRTGSCVGEPQANALHCLLNRE----LTVFTDVLNDTDTENRSQSFPLPDDHWINQAFPEEDD-KTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGID-VNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKAAN---RKGEGPKLLEDFRSL---LLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKV-SLDLTNV-------RTDETILHCLLKGGRRN------DEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDD-CIEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPAL-------------------------------FILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTAL-DG--LNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPN-DDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPNVRKSRCDCLRVELMDDDIIARTKEIVLQKATPNPEEDRWQKLEGKLGLEIVGLN-----ASVGARMANLEAKMDLLLSSLSKR 1040
             +KK     +F+R GSC+ +PQ N L C+L+++     +V  +V  D D    SQ++PLP DHWINQ   +  + +TLL +A+E+       +LL+ GA   L+N  LG +PVHVA    +L  L  L     N  +INA MR +GRT LH+  +K   E + F+L+   +  V+ +D +  QT LY++VK  ++   + L+E GA        KTI  E M + +P F+P  V+ K   I ++  +    +L  IL+KA     +     + L  F++    L+  D   L++    G T LQK+C+ G   +V  +L  E   +P+   E  +  P+LLAA  GH G ++ L+Q K+ SL+   V       +T ET+LH +L    R       D     +LL    + ++  ++ +I+N  D   NTALHYATQ+W Q  VR LL+ GANIG+ N W+ETP++KILP+T+E++LD+ C+ +  DV HE FE+ + YSFLAPP +   F           DPE +++ E       ALPET++LW+M  S+ HRHLLKHPV+TSFL++KW RIR+YFNR LR YLLFV  L+W+IFE +GG S R +   D  +                                ++++   +++ +  ILRDW  D++                S +K A  V+F++W+E   +  +  ++      L + L  L +++ +RE  Q+ VSLKRYI   EN  E+ M+ L+   L+  +  L +G  L RH +   LV++WA LITLVG+HPKL+ YN+YVTMFYKVL +F  FL WY FFIIAFGLGFYIMLH+D P      G D+Y FF  PW +LVKT TMF+GE+EFS+IPI+L +++G L Y F + F+FLIVVVLMNLLNGLAVSDTG+I+E+AEIV+YISRVETIS TE++LLGDPFNFL+ WPA+ +L ++PS + C  L ++  ++ I NKITGATGILLFYT+LP K+L LQPN R +R  C+  + M  +II   K+IV++K            LEG   +  + LN      ++  ++ NL  K+DL+L+ L+ R
Sbjct:   17 QEKKNTKKSEFIRVGSCISDPQTNVLECVLHQDHDGLTSVLAEVAPDEDGVEASQNYPLPPDHWINQPTSKLGNFRTLLHIAIEQIDLKSVCLLLRGGARADLHNEDLGVSPVHVACQVNSLDILAELVRDPRNSGNINAGMR-DGRTCLHIAADKNRLEILQFILENKALQTVDPEDLRGRQTPLYLSVKKKNIEAVRLLLENGAQLLNTVDNKTI-REHMKENIPNFNPESVQIK-IKIKERNGEDVAYSLNRILDKAQMNIIKNLPNSQELIQFKTFIQSLIAMDKKLLDSYDCGGFTFLQKSCEYGFSEFVHILL--ENGAHPNQTMETNSTKPVLLAAYGGHQGVLQVLLQHKIDSLEGGQVVNLTVQEKTKETVLHYVLSKPSRGPRIQGGDYPGCLNLLLECKDRSVVDELNRIMNWSDRQGNTALHYATQLWTQETVRKLLERGANIGVKNRWSETPISKILPETMESFLDEFCLTSTNDVQHEDFELKFRYSFLAPPVDSPNF--------DEHDPEGREMVENR-----ALPETESLWYMADSQYHRHLLKHPVITSFLWMKWQRIRKYFNRGLRVYLLFVTCLSWFIFEAYGGVSYRVNSKTDTNSSSPESTESTVEEKEQRSLCSVNLEPTESRGFWYVVFILQSIIQIFMILRDWYRDLRQ---------------SDLKTAFQVLFTSWIEYFILSSIVVLIILSTQALVYILTILILMVALREVLQMMVSLKRYIFDIENLFELVMLALVCICLYVPEHYLYNGCLLKRHLAGFALVIAWATLITLVGRHPKLSRYNIYVTMFYKVLKSFILFLTWYSFFIIAFGLGFYIMLHRDVPGYTPKEGEDEYKFFEDPWLSLVKTGTMFIGEIEFSNIPIDLTTKVGILGYIFLICFIFLIVVVLMNLLNGLAVSDTGIIREEAEIVTYISRVETISCTEAVLLGDPFNFLANWPAIAFLRDVPSMAFCRSLYRNSLVRNISNKITGATGILLFYTWLPDKELVLQPNRRTNRFVCMPTDCMGAEIIQAAKDIVIKKTRLG--------LEGPGEISDLLLNLQKNQENLQDQILNLATKLDLVLTKLNTR 1077          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325320373|ref|XP_023341984.1| (transient receptor potential cation channel protein painless-like isoform X1 [Eurytemora affinis])

HSP 1 Score: 757.673 bits (1955), Expect = 0.000e+0
Identity = 450/1104 (40.76%), Postives = 650/1104 (58.88%), Query Frame = 0

Query:    6 SQKKKPTVGDFVRTGSCVGEPQANALHCLLNRE----LTVFTDVLNDTDTENRSQSFPLPDDHWINQAFPEEDD-KTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGID-VNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKAAN---RKGEGPKLLEDFRSL---LLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKV-SLDLTNV-------RTDETILHCLLKGGRRN------DEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDD-CIEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPAL-------------------------------FILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIV--MFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTAL-DG--LNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPN-DDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPNVRKSRCDCLRVELMDDDIIARTKEIVLQKATPNPEEDRWQKLEGKLGLEIVGLN-----ASVGARMANLEAKMDLLLSSLSKR 1040
             +KK     +F+R GSC+ +PQ N L C+L+++     +V  +V  D D    SQ++PLP DHWINQ   +  + +TLL +A+E+       +LL+ GA   L+N  LG +PVHVA    +L  L  L     N  +INA MR +GRT LH+  +K   E + F+L+   +  V+ +D +  QT LY++VK  ++   + L+E GA        KTI  E M + +P F+P  V+ K   I ++  +    +L  IL+KA     +     + L  F++    L+  D   L++    G T LQK+C+ G   +V  +L  E   +P+   E  +  P+LLAA  GH G ++ L+Q K+ SL+   V       +T ET+LH +L    R       D     +LL    + ++  ++ +I+N  D   NTALHYATQ+W Q  VR LL+ GANIG+ N W+ETP++KILP+T+E++LD+ C+ +  DV HE FE+ + YSFLAPP +   F           DPE +++ E       ALPET++LW+M  S+ HRHLLKHPV+TSFL++KW RIR+YFNR LR YLLFV  L+W+IFE +GG S R +   D  +                                ++++   +++ +  ILRDW  D++        +S+ + A   V   LIV  +F++W+E   +  +  ++      L + L  L +++ +RE  Q+ VSLKRYI   EN  E+ M+ L+   L+  +  L +G  L RH +   LV++WA LITLVG+HPKL+ YN+YVTMFYKVL +F  FL WY FFIIAFGLGFYIMLH+D P      G D+Y FF  PW +LVKT TMF+GE+EFS+IPI+L +++G L Y F + F+FLIVVVLMNLLNGLAVSDTG+I+E+AEIV+YISRVETIS TE++LLGDPFNFL+ WPA+ +L ++PS + C  L ++  ++ I NKITGATGILLFYT+LP K+L LQPN R +R  C+  + M  +II   K+IV++K            LEG   +  + LN      ++  ++ NL  K+DL+L+ L+ R
Sbjct:   17 QEKKNTKKSEFIRVGSCISDPQTNVLECVLHQDHDGLTSVLAEVAPDEDGVEASQNYPLPPDHWINQPTSKLGNFRTLLHIAIEQIDLKSVCLLLRGGARADLHNEDLGVSPVHVACQVNSLDILAELVRDPRNSGNINAGMR-DGRTCLHIAADKNRLEILQFILENKALQTVDPEDLRGRQTPLYLSVKKKNIEAVRLLLENGAQLLNTVDNKTI-REHMKENIPNFNPESVQIK-IKIKERNGEDVAYSLNRILDKAQMNIIKNLPNSQELIQFKTFIQSLIAMDKKLLDSYDCGGFTFLQKSCEYGFSEFVHILL--ENGAHPNQTMETNSTKPVLLAAYGGHQGVLQVLLQHKIDSLEGGQVVNLTVQEKTKETVLHYVLSKPSRGPRIQGGDYPGCLNLLLECKDRSVVDELNRIMNWSDRQGNTALHYATQLWTQETVRKLLERGANIGVKNRWSETPISKILPETMESFLDEFCLTSTNDVQHEDFELKFRYSFLAPPVDSPNF--------DEHDPEGREMVENR-----ALPETESLWYMADSQYHRHLLKHPVITSFLWMKWQRIRKYFNRGLRVYLLFVTCLSWFIFEAYGGVSYRVNSKTDTNSSSPESTESTVEEKEQRSLCSVNLEPTESRGFWYVVFILQSIIQIFMILRDWYRDLR--------QSDLKTAFQDVLHPLIVKVLFTSWIEYFILSSIVVLIILSTQALVYILTILILMVALREVLQMMVSLKRYIFDIENLFELVMLALVCICLYVPEHYLYNGCLLKRHLAGFALVIAWATLITLVGRHPKLSRYNIYVTMFYKVLKSFILFLTWYSFFIIAFGLGFYIMLHRDVPGYTPKEGEDEYKFFEDPWLSLVKTGTMFIGEIEFSNIPIDLTTKVGILGYIFLICFIFLIVVVLMNLLNGLAVSDTGIIREEAEIVTYISRVETISCTEAVLLGDPFNFLANWPAIAFLRDVPSMAFCRSLYRNSLVRNISNKITGATGILLFYTWLPDKELVLQPNRRTNRFVCMPTDCMGAEIIQAAKDIVIKKTRLG--------LEGPGEISDLLLNLQKNQENLQDQILNLATKLDLVLTKLNTR 1086          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325325088|ref|XP_023344392.1| (transient receptor potential cation channel protein painless-like [Eurytemora affinis])

HSP 1 Score: 703.745 bits (1815), Expect = 0.000e+0
Identity = 423/1042 (40.60%), Postives = 596/1042 (57.20%), Query Frame = 0

Query:   21 SCVGEPQANALHCLLNRELTVFTDVLNDTD----------TENRSQSFPLPDDHWINQAF----PEEDDKTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRS-LQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKAANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQ-----------SKVSLDLT--NVRTDETILHCLLKGGRRN--------------DEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDDCIEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDE--RVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSE------AQDIPAL-------------FILYAAFALVLVAFILRDWAM---DIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDT---ALDG---LNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQL-SKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPNVRKSRCDCLRVELMDDDIIARTKEIVL 989
            SC G+PQ N  +C+L+ +LT F+ +L D            +++  + +PLP++HWINQ      P    + LL +++++   +FT++L++ GA   +YN    +A +H A LS +    L  L S + N+ DIN T++ +  T LHL  E+    C+N LL+ P I ++ +D   G+T L++A +  H+   + LVE GA  D  C G+T    ++ + LP    ++++ K  P+       T E L   +E+         K    FR  L +     ++ +   G T LQKA    L ++V ++LN  + VNP+  +    + P+LLA+ RGH   +   V+           SKVS +    +  T ET LH +LK                   DE + + L  +L +E +RSQ+ ++IN+RD+L  T LHYA QMW Q  +  LL LGANIG+ N   E P+++ILP+TLE +L+ C   E    H+ F+V +NYS+LAPP +D                +S++  +   DE  R  LPET++LW M QSK+HRHLL+HP VTSFL+LKW R+RR+F+RN+R YLLFV  LTWYIF RFGG  L  SE      A D PA+             F   A     +V F++    +    I+DL R    KS  +         +I   S+W E      +  +   GE  LW  +  L + L +RE FQ++ SLKRY  S EN +E++M+ L+G +LF  DT     DG   + RH +A+ ++LSW E+I LV KHP+L+ YNVY++MFYKVL TFF FL WY FFIIAF LGFYIMLHKD PN      D YV+F+ PWT+LVKTSTMFVGELEFSDIPI+ +S L  LS+ F L FVF IVV+LMNLLNGLAVSDTG+I EKAEIVSY +RVETISY ES+LLGDPF FLS WP +  L  +PS +LC Q+ S     +++ + +TGATGILLFY+ LP KK +     ++    C  V+ +  DI+   +++ L
Sbjct:   28 SC-GDPQENLSNCILSGDLTTFSCILADIAPLDDHHQLSLSDDEKKVYPLPEEHWINQPLISDNPNLAQQLLLQVSVQQGAVEFTQLLVRAGARANMYNDETNQAVIHTAVLSQHGEDHLTALLSDEKNKADINCTLQPSSLTPLHLAAERGKLTCLNLLLEHPDIQIDPRDGSGGRTPLFLAARGKHVECVRKLVEQGASLDIKC-GRTTPRTVIQENLPYLDLSKIKVKERPVA-----NTVETLFQYIER---------KDFLRFRYTLTKVSLRDVSEKSVRGYTCLQKAVLANLPTFVTQLLN--HGVNPNLYSVENPVRPVLLASYRGHYQVLSVFVEELETALSLGKVSKVSPNFAVWSRDTQETCLHMILKKSHSKALLGLGSDSELKGLDEDYRKCLNILLSSEQVRSQLGRVINKRDILSYTPLHYAAQMWSQADITKLLKLGANIGVRNSRGEIPLSRILPETLEEFLNHCSNHENHPLHQDFKVEFNYSWLAPPVDDY---------------QSEEWDQHRQDELERAGLPETESLWCMSQSKSHRHLLRHPTVTSFLWLKWQRVRRFFSRNIRIYLLFVTCLTWYIFTRFGGIKLNSSEPSLDTGALDPPAVSSSNSTQFCKHPRFDSGAQLGFWMVLFVIESIFLLIYAIRDLFRECGCKSGTD--------FMIAFLSSWFEMFLAALIILIFITGEGGLWWVITLLFVTLTLREIFQMTASLKRYFFSLENLLEIAMLGLLGVLLFAPDTLDPTEDGDCVVLRHIAAVCILLSWTEMIILVAKHPRLSRYNVYISMFYKVLQTFFLFLVWYSFFIIAFALGFYIMLHKDIPNF-TPDSDHYVYFDSPWTSLVKTSTMFVGELEFSDIPIDTESNLAWLSFTFLLVFVFFIVVILMNLLNGLAVSDTGIIMEKAEIVSYTARVETISYMESVLLGDPFLFLSHWPRVSCLARLPSLALCNQVYSACPGARKVGHCLTGATGILLFYSLLPEKKASFPMEDKEGCGGCRVVQDIPQDILQAARDLAL 1027          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325279050|ref|XP_023324174.1| (transient receptor potential cation channel protein painless-like [Eurytemora affinis])

HSP 1 Score: 670.233 bits (1728), Expect = 0.000e+0
Identity = 424/1035 (40.97%), Postives = 594/1035 (57.39%), Query Frame = 0

Query:   30 ALHCLLNRELTVFTDVLNDTDTEN--RSQSFPLPDDHWINQAFPEEDDKTLLMLALEKDLHDFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMR--SNGRTALHLCVEK---KHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCFGKT--IFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKAANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGGRRNDEHFVQDLLDILGNE---AIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLD-DCIEAEGDVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRP----SPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGG----KSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIV--MFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDY---VFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPNVRKSRCDCLRVELMDDDIIARTKEIVLQKATPNPEEDRWQKLEGKLGLEIVGLNASVGARMANLE----AKMDLLL 1034
            AL  L   +L  FTD+LN  D E   R Q        WI      E    L+ L +E+D  +F + L++ G    L N I G AP+H A L G    ++L       + D+N+       G TALHL  +     H +C++ LL   GIDVN  D  +  + LY+A K  +    + L+E GA +D +  G+T     + + + LP      +R K P   +Q      E +  I+   +       +L  +FR L     S ++++       L++K C  GL  +   + N+    +P+         PIL AA R      + LV++   +     RT+ET+LHCLLK   ++ E      L+IL N+    IR  I  ++N++DL   T LHYAT+   Q VVR+LL  GANIG+ N W E P+++I P+ LE +LD DCI  +G+V+ ++F +T+ YSFLAP  + LP   R  L       P + E  K+ + + + + ALPET++LW+MGQSK HRHLLKHPV+TSFL+ KW R+R YFNRNLR YLL+VFILTW+IFE+FGG     ++RD       A F+LY A  ++    I++DW  D+K+++ S + +  D    SS +   +V  M SN  + L +  +  + +     L   L+ L  +L++RE  Q+ VS+KRY+ S ENWIEV+MI+L+ +IL  N      +NRH +AI LV SW ELIT++G+HPKL   N+YVTMFYKVL +F  FL W+  FI+AFGLGFYIMLH D   D ++G +D      FN+ W +LVKTS MFVGELEFSD+PI+    LG L+Y +FLSFVFLIVVVLMNLLNGLAVSDTG I+ KAEI SYISRVETISY ES LLGDPF+FLS  P  ++L +IPS SL  Q  ++   +++F   TGA G LLFYT LP K+ T++PN   + C  L+ + + + I+   KEIVL+    N  +D +   E           A +  ++ NLE     K+DLL+
Sbjct:    7 ALVALKGNDLQAFTDILNTKDVEEGVRDQV------SWIYSPIKSEKSLNLVELCVEEDNLEFIRTLVRLGVHSHLLNTITGYAPLHRAVLLGKHEIVKLFIDNSRTEFDVNSRSGRLKRGYTALHLVADNPTDNHLQCLDILLGCEGIDVNCLDCSSTVSPLYLAAKKKNESAVRKLIENGAAFD-IKVGRTSKTVEDFIKENLPQLQLRTIRVKKP---RQREDNVKERMITIVSNVSPDSLNLHQLFLEFRHL-----SLSVHDASRGFEDLVEKCCRKGLHDFAQVLFNKN--ADPNRFGSGSLSCPILDAAERSDYRLAQVLVRAGAEMSQVKERTEETVLHCLLKNYNKDIEEDFSRFLEILFNKDDIKIRRSIDLVLNRKDLTGVTPLHYATEKCSQNVVRALLRRGANIGIKNMWGEIPISRICPRLLEEFLDQDCIIGDGEVHDKEFSLTFKYSFLAPDPQSLPEPYRPPLELDEALQPLNQEVNKINDNK-ERKTALPETESLWYMGQSKQHRHLLKHPVITSFLWFKWERMRPYFNRNLRLYLLYVFILTWFIFEQFGGVGQKPAIRD-------AFFVLYIALCILTTLLIIKDWTRDLKEIISSPQKEDPDYGVRSSRRGQGLVSQMTSNIPDILQISGMVCIFFIQPSHLNIPLIVLLCILVLRELLQLLVSMKRYLCSLENWIEVAMIVLVVYILCNNTEDDFAVNRHLAAIALVFSWTELITMIGRHPKLHHLNIYVTMFYKVLNSFLLFLMWFSMFIVAFGLGFYIMLHTDTVTD-ISGLEDEDKNPLFNKTWLSLVKTSAMFVGELEFSDLPIDSSGYLGMLAYCYFLSFVFLIVVVLMNLLNGLAVSDTGDIKAKAEIYSYISRVETISYMESTLLGDPFDFLSNVP--EFLKSIPSISLIRQCYRTGFCRKVFK--TGAKGFLLFYTVLPDKEFTVKPNHLGAMCS-LQQDQIGEGILVSAKEIVLK----NRSQDSYDTTEL----------ADLREKLFNLETEINGKLDLLI 996          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325314367|ref|XP_023338859.1| (transient receptor potential cation channel protein painless-like [Eurytemora affinis])

HSP 1 Score: 442.195 bits (1136), Expect = 2.870e-135
Identity = 338/992 (34.07%), Postives = 503/992 (50.71%), Query Frame = 0

Query:   18 RTGSCVGEPQANALHCLLNRELTVFTDVLNDTDTENRSQSFPLPDDHWINQAFPEEDDKTLLMLAL-EKDLHD-FTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHG----------ECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCFGKTIFPELMADKLPGFHPNQVR--RKNPPIVQQLSQGTFEALADILEKAANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGS-TLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQ--SKVSLDLTNVRTDE-----TILHCLLK-------GGRRNDEHFVQDLLDIL--------GNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDDCIEAEGDVNHEKFEVTYNYSFLAPPKED----LPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHAL--LFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPNVRKS 966
            R  SCVG+PQ  AL    +++   F D +++   +              N+ + E+  KTL  L L E +  D F   LL+ G    L N  L   P H A    +   L++  SL G Q ++N      G TALH+  E+             E ++ L+  PGID+N +D K   T LY A  A+       L+  GA+ +       +  E+  + LP F+ +  +  R   P+           L +++E A N        LE F +     D    N+   NG  TLLQ A + G       +L    + N   +T +   PP +LAA+ G+   ++   +  +   + +  + TD+     TILH +++          R+     ++ L +L        G        ++IIN RDL   TALHYATQ   Q +++ LL  GAN+G+ N   + PV KILP TL+ +LD+C+ ++G +  +   +T++Y+FLAPP  D    L F +++D+  S         A G+T  R   PET+ LW++ +SK HR LLKHPV++SFL +KW RIR ++  +L  Y  FV  LT  I   +GG SL                 F+ V         + +I+  V+ E+   +  + +SS +                         G+  +W  +  +FLT L +I E  Q++VS+KRY  S  N+I+V++++    ++F    + D   RH +A++++ SW+E I LVG+HP L+T    VTM + V+ TFF FL WY  FI+AF   FYIM H D  + +V    +Y FF+   T +VKTS MFVGELEFSDIP + +     +SY  F++F+FLIVVVLMNLLNGLAVSDTGLI+E+AE++S  S+V+ ISY ESILL DP+NFL+ WP  K+L  +PSCS C  + +   ++   +KITG   ILLFY  LP K  T  PN  K+
Sbjct:   20 RVQSCVGDPQEEALSAYESKDYKTFVDCVSEEGFDP-------------NKEYGEDIPKTLSHLILDEAEGRDIFLLALLKNGGRTDLRNKTLETIPFHEAIRKKDPALLKV--SLLGVQ-NVN-IQDGEGSTALHVATEELQNADESELDNLLEIISILIQVPGIDLNHEDLKGKCTPLYYAAAAASEKAVDLLLCYGANSEI-----EMIQEVFKENLPDFNISNYKTARTGRPLKN--------VLFNLIELADN--------LEGFANFTYNRDGIDWNS--DNGQFTLLQYAAEQGRAKICGYLLENGADPN---LTGSNDTPPWILAAHSGYYKVLQVFFEKLTDREIRMAMITTDQHHGQRTILHEVVRQHSPKLDNPYRDTNIDYEECLKVLFEDRKNMRGKAKYSKYTERIINFRDLHGETALHYATQQPNQAIIKLLLKHGANMGVKNKEEKPPVNKILPSTLQEFLDECVASQGIITDDDLRLTFDYNFLAPPLLDKEVLLNFETKRDVEGS---------AVGDTHPR---PETEALWYLSESKDHRGLLKHPVISSFLLMKWQRIRTFYIISLLIYFFFVLFLTLLILLEYGGCSL-----------------FSSV---------STNIESEVKEEQEGLDKADESSSCR-------------------------GDTTVWKVIVGIFLTFLAII-ELLQIAVSVKRYFASSTNYIQVAILVGGAILIFDRSMSWDE-RRHLAALLIIFSWSEFIILVGQHPSLSTN---VTMLFTVVSTFFKFLLWYTAFIVAFAFSFYIMFHTDHKDGEVN--PEYKFFDEIGTCIVKTSAMFVGELEFSDIPFSAN----PISYIIFVAFIFLIVVVLMNLLNGLAVSDTGLIREEAEVLSLKSQVDIISYWESILLNDPYNFLTSWP--KFLSALPSCSCCFWVKRIPLLETFLSKITGGARILLFYECLPDKSATFYPNSGKT 892          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325287472|ref|XP_023328489.1| (transient receptor potential cation channel protein painless-like isoform X1 [Eurytemora affinis])

HSP 1 Score: 346.665 bits (888), Expect = 2.159e-106
Identity = 206/392 (52.55%), Postives = 266/392 (67.86%), Query Frame = 0

Query:  651 VECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPN-VRKSRCDCLRVELMDDDIIARTKEIVLQKATPNPE---EDRWQKLEGKLGLEIVGLNASVGARMANLEAKMDLLLSSLS 1038
            +E L ++F+  +L  G  IL   L+ L ILL++RE  Q +VS KRYI S EN +E+ +I L   ++   D     LNRH +AIVL+LSW ELI LVG HPKL   N+YVT+F+KVL TFF+FL WY  FI+AFG GFYI+LH+D         D Y FFN+ W +LVKTSTMFVGELEFSD+P +LDS L  LSY FFLSF+FL+VVVLMNLLNGLAVSDTG+I+EKAEI SY S+VETIS  ES+LLGDPF+FLS  P +  +  IPSCSL  QL ++RT+  +F KI G++ ILLFY +L  K++T++PN + K+ C  L  + M  DII+  KEI+L+K T   +   EDR +++E K               M  LE K+D +L+ LS
Sbjct:    2 IEVLMIIFMCFILIFGSAILETCLIILLILLILRELLQTTVSFKRYIFSIENIVEICIISLTIVLITDKDGERFELNRHLAAIVLLLSWGELIVLVGVHPKLKHCNIYVTIFFKVLRTFFWFLAWYSLFIVAFGFGFYIILHQDTGVIQEPEDDPYPFFNQTWLSLVKTSTMFVGELEFSDLPFDLDSPLAPLSYIFFLSFIFLMVVVLMNLLNGLAVSDTGIIREKAEIFSYRSKVETISTLESMLLGDPFDFLSNVPEM--VSRIPSCSLLRQLYRNRTLGDLFKKI-GSSEILLFYRYLENKQITIKPNQINKTCCRGLSTDEMGRDIISSAKEIILRKNTRTEDKVLEDRMERIEDK---------------MQGLEDKLDKILAKLS 375          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325287474|ref|XP_023328490.1| (transient receptor potential cation channel protein painless-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 315.849 bits (808), Expect = 1.476e-95
Identity = 184/323 (56.97%), Postives = 233/323 (72.14%), Query Frame = 0

Query:  651 VECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNFLSQWPALKWLLNIPSCSLCAQLSKSRTMQRIFNKITGATGILLFYTFLPTKKLTLQPN-VRKSRCDCLR 972
            +E L ++F+  +L  G  IL   L+ L ILL++RE  Q +VS KRYI S EN +E+ +I L   ++   D     LNRH +AIVL+LSW ELI LVG HPKL   N+YVT+F+KVL TFF+FL WY  FI+AFG GFYI+LH+D         D Y FFN+ W +LVKTSTMFVGELEFSD+P +LDS L  LSY FFLSF+FL+VVVLMNLLNGLAVSDTG+I+EKAEI SY S+VETIS  ES+LLGDPF+FLS  P +  +  IPSCSL  QL ++RT+  +F KI G++ ILLFY +L  K++T++PN + K+ C  LR
Sbjct:    2 IEVLMIIFMCFILIFGSAILETCLIILLILLILRELLQTTVSFKRYIFSIENIVEICIISLTIVLITDKDGERFELNRHLAAIVLLLSWGELIVLVGVHPKLKHCNIYVTIFFKVLRTFFWFLAWYSLFIVAFGFGFYIILHQDTGVIQEPEDDPYPFFNQTWLSLVKTSTMFVGELEFSDLPFDLDSPLAPLSYIFFLSFIFLMVVVLMNLLNGLAVSDTGIIREKAEIFSYRSKVETISTLESMLLGDPFDFLSNVPEM--VSRIPSCSLLRQLYRNRTLGDLFKKI-GSSEILLFYRYLENKQITIKPNQINKTCCRGLR 321          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1233190500|ref|XP_022196180.1| (transient receptor potential cation channel protein painless [Nilaparvata lugens] >gi|1233190503|ref|XP_022196181.1| transient receptor potential cation channel protein painless [Nilaparvata lugens])

HSP 1 Score: 327.405 bits (838), Expect = 2.801e-92
Identity = 284/908 (31.28%), Postives = 422/908 (46.48%), Query Frame = 0

Query:   16 FVRTGS-CVGEPQANALHCLLNRELTVFTDVLNDTDTENRSQSFPLPDDHWINQAFPEEDDKTLLMLALEKDLHD-FTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATMRSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAVKASHLVITKALVEAGADYDYVCF--GKTIFPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKAANRKGEGPKLLEDF-RSLLLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGG----------RRNDEHFVQDLLDILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDDCIEAEGDVNHE-KFEVTYNYSFLAPPK----EDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERFGGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLI-REFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTALDGLNRH-FSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELEFSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDP 901
              RTGS C  +P    L    +R+   F  +L    T     ++  P+  W    F + +  TLL LA  K     + + LL  GA     N +  ++P+H A   G++  + LL  L  N+ D+N  +   G TALH      + + +  LL  P I+ NI ++K G T +       H+ +TK   EA      +C    + I P    D +    PN V +         + G    L ++L K            E F R++ + TDS   N+      T LQ ACD G+   V  +L      +P+G   A    PI+LA+ +G+   ++ LVQ+  +    N    ET+LHC++KG           + + +H+    +++L       ++   IN  D+  NT LHYA ++  +  +  LL  GA +G  N   E  +  + P+  E YLDDC+     +  E  +E+ + Y+FLAPPK    EDL   SR  L+       + K  +  TD+ ++  ET+ L +M +S   R LLKHPV+TSFL+LKW RIR +F  NL FY+ F   LT YI   + G    + E  D                  I  +     K L++    ++  E      KF             FVL+L              L+ L++++LI RE FQ++VS  RY  +PENW+EV +I +   ILF         +RH  SA+ ++LSWAEL+ L+G+HP L+T    + M   V   F  FL WY   IIAF L F+ +        D     D  FF  P  ++ KT  M  GE +   IP          S+  F+ FVFLI +VL NLLNGLAVSDT  I+  AE+V Y+SRV+ ++Y ES+++G P
Sbjct:   13 LARTGSLCSPDPHDQLLTAFNDRDFDNFQKIL----TTGVKNAYVDPN-FW----FDDPNYCTLLDLACRKSSSSQYVRCLLSCGADPNKINSVRKKSPIHFAVELGDISIVNLL--LHDNRTDVNK-LDGTGSTALHAAARSSNVDVMIALLGHPKINPNIANRK-GLTPI-------HVAVTKGTEEA-----VLCLLQHQNIDPRSATDLILESFPNLVPKLPTTGNTVETLGKSNNLFNLLHKHDE---------ETFVRAIQINTDSINENDGF---HTFLQYACDFGMFYTVGHLL--RLGADPNGTHTANRTTPIMLASYKGYYNIVRLLVQNDCTR--YNPINGETVLHCVIKGSTDLHVSLGASKEDRDHY--KCMEVLLRGVPGHKLN--INCGDVKGNTPLHYAAKLNNKEFILLLLSSGAYVGQRNTLGEPALADVSPKIFEKYLDDCLTTNDKLPREDNYEIIFRYNFLAPPKVQKHEDLT-SSRAMLQIE----SNMKGVDEPTDQVIS--ETEPLLYMSRSSDLRPLLKHPVLTSFLHLKWHRIRCFFYCNLTFYIFFWMFLTAYILTAYAGMR-PEGEGNDK-----------------INTNTTSFNKTLMKRSTQETIPETIGDEFKF-------------FVLWL--------------LVALSLVILILRELFQLTVSPLRYFANPENWLEVCLIGVTLSILFCRSCV---NSRHQISAVAILLSWAELVLLIGRHPALST---NIEMLKTVSWNFLKFLAWYSILIIAFALSFFTLF------SDSETEGDENFFVDPANSVFKTVVMLTGEFDAGSIPFVTHP---GTSHVLFVLFVFLIAIVLFNLLNGLAVSDTQSIRADAELVGYVSRVKLVTYVESLVMGTP 808          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|328724582|ref|XP_001950177.2| (PREDICTED: transient receptor potential cation channel protein painless [Acyrthosiphon pisum])

HSP 1 Score: 323.939 bits (829), Expect = 1.181e-91
Identity = 264/842 (31.35%), Postives = 396/842 (47.03%), Query Frame = 0

Query:   89 DFTKVLLQTGASGRLYNHILGRAPVHVAALSGNLRSLQLLFSLKGNQPDINATM-RSNGRTALHLCVEKKHGECVNFLLDQPGIDVNIKDKKNGQTALYMAV--KASHLVITKALVEA-GADYDYVCFGKTI-------FPELMADKLPGFHPNQVRRKNPPIVQQLSQGTFEALADILEKAANRKGEGPKLLEDFRSLLLQTDSFTLNNRVTNGSTLLQKACDLGLVSYVAEILNEEYEVNPDGVTEARAIPPILLAANRGHIGCIKELVQSKVSLDLTNVRTDETILHCLLKGGR--RNDEHFVQDLLD------ILGNEAIRSQIQKIINQRDLLENTALHYATQMWPQTVVRSLLDLGANIGMTNHWNETPVTKILPQTLEAYLDDCIEAEG----DVNHEKFEVTYNYSFLAPPKEDLPFVSRQDLRPSPSDPESQKLAEGETDERVALPETQTLWHMGQSKAHRHLLKHPVVTSFLYLKWSRIRRYFNRNLRFYLLFVFILTWYIFERF--GGKSLRDSEAQDIPALFILYAAFALVLVAFILRDWAMDIKDLVRSEKVKSEDEEATSSVKFALIVMFSNWVECLFVLFLGAVLWGGEPILWHALLFLTILLLIREFFQVSVSLKRYIVSPENWIEVSMIILIGFILFWNDTALDGLNRHFSAIVLVLSWAELITLVGKHPKLTTYNVYVTMFYKVLGTFFFFLCWYVFFIIAFGLGFYIMLHKDGPNDDVAGPDDYVFFNRPWTALVKTSTMFVGELE-FSDIPINLDSRLGALSYAFFLSFVFLIVVVLMNLLNGLAVSDTGLIQEKAEIVSYISRVETISYTESILLGDPFNF 904
            +F ++L   G    + N    +AP+H+AA +G   +L++L       P  N  +  S+G +ALHL  +  + EC   LL+   I  N  ++K G T  Y+A   K  +  +  A +     D D   + K++       +P L  +  P    N     + P V   S                        +E F S +  T    LNN      T LQ ACD GL   V  +LN   +V+P+G+  +     I +A+ RG    I+  + S+  +      ++ T L  ++KG     N      DL D      +L  ++  + ++  INQ D   NT LHYA ++  +  + SLL+ GA IG  N   + P+  I P  LE++L+ CI++ G    D+N   +E+  +Y+FL PPK       ++D+         Q   EG       +PET  L ++ +    R LLKHPV+TSFL+LKW  I+RY+  NL FY+ F  +LT Y    F  GG++   S  Q+                                     +  E +T+SV F+          C   ++           LW  +L   I L+ RE FQ+  S   YI+S ENW+E+S+I+L  F+L  + + +   N+  SAIV++LSW ELI LVG+HP L    +++ MF +V   F  FL WY   IIAF L FY++   D   D+         FN P +A  KT  M  GE + F  +P +L      +S+A FL F+FLI +VL+NLLNGLAVSDT  I+  AEIV+++SRV+ I Y E + LGDPF++
Sbjct:   74 EFVRILCSVGVDVNVINKNRKKAPIHLAAANGCKDALEVLLE----HPPTNINLLDSDGNSALHLAAKSGNAECSELLLNSNNIKANQLNRK-GFTPAYLAATSKKKNDELMMAFIRCPKVDLDMFIWDKSLRSVIEEKYPHLSEEMPPEGTRNITHHCDNPFVYLYSND----------------------IEGFISHITSTKQLDLNNH-DGKHTYLQYACDFGLPKVVQVLLN--LDVDPNGICPSNTKYAIFIASFRGFADIIQLFLNSRKCICYQT--SNSTALLEVIKGSDEDHNSLDVNTDLCDHERSFKMLLRKSETNPLELNINQSDDKGNTCLHYAAKIGDRDSILSLLEKGAYIGKRNVLGDPPLADISPNILESHLNKCIKSNGKSPKDIN---YEIICSYNFLCPPK----VYKQKDID------NYQSQLEGLEYIPENIPETDPLLYISEVPELRPLLKHPVLTSFLHLKWFAIKRYYKVNLAFYIGFWALLTIYTLLTFKIGGQNSNLSLTQN-------------------------------------NITEFSTNSVPFS----------CDTAVY-----------LWVMVLGFLIALIFRELFQLISSPFTYIMSLENWLEISLIVLTSFLLLRDMSCISNRNQ-ISAIVILLSWGELILLVGRHPALA---IHLEMFKRVTKNFLQFLVWYSILIIAFALSFYMLFRNDADTDNK--------FNYPSSATFKTIVMLTGEFDTFGSLPFSLHP---IVSHALFLLFIFLIAIVLVNLLNGLAVSDTQAIRADAEIVAHVSRVKLIHYFEKMALGDPFSW 797