BLAST of hypothetical protein BRAFLDRAFT_212201 vs. L. salmonis genes
Match: EMLSAG00000004175 (supercontig:LSalAtl2s:LSalAtl2s220:372133:389968:-1 gene:EMLSAG00000004175 transcript:EMLSAT00000004175 description:"maker-LSalAtl2s220-augustus-gene-3.30")

HSP 1 Score: 60.8474 bits (146), Expect = 8.891e-11
Identity = 58/247 (23.48%), Postives = 110/247 (44.53%), Query Frame = 0

Query:  169 GFVL---LQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVR--DTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYI---------KFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVLQ 401
            GF +   ++++ L+EG +  + ER+   +   +G G+    +  D  S+ +Y+   +  H D+TY  +  G+ + H +K+ E  GGET L DGF+ A   K    + Y   +      R   ++YI          ++   I +   +    R  R N  + A H  L   +++   FY+  +    +        + K+ PG +I + N R++HGR A  G    R +   Y++  +  S+ +V +
Sbjct:   18 GFAMVHGIKDMSLQEGTLATV-ERMVRIQSNLFGRGFQFSSKRVDDVSDSSYSQDFLLPHNDMTYLTHASGLQVFHPLKESE-MGGETILVDGFEIARQFKELCAEGYSYFSYE----RPVESEYIHKGEKPHEHSYSSDFIFKHYPNSSVLRQVRYNVYDRAPHKGLR-TVEEQMKFYEFYKLLSSVFQKPSNSFELKLVPGTLIFIDNFRVLHGRNAFRGS---RIMSGCYVERSDFLSQAKVFE 254          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. SwissProt
Match: gi|21541962|sp|Q9QZU7.1|BODG_RAT (RecName: Full=Gamma-butyrobetaine dioxygenase; AltName: Full=Gamma-butyrobetaine hydroxylase; Short=Gamma-BBH; AltName: Full=Gamma-butyrobetaine,2-oxoglutarate dioxygenase)

HSP 1 Score: 237.654 bits (605), Expect = 1.327e-73
Identity = 135/387 (34.88%), Postives = 209/387 (54.01%), Query Frame = 0

Query:   20 LHRRFLGTSINRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKFTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQV-ELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALE-GKLTGRHLQCGYIDLDEVDSRIRVLQSEI 404
            +H   L        +++Q  + DG +  +P VWLRD+CQ S  +  S+ +R   L    ++I+      + K K  ITW +GH S F   +L+   F  EARA  +    + E + W  +     P  +F  V+        WL  L K G V L     + G I ++ +R+ F  LT YG  + V  +   +N+AYT   + FHTD     + PGV LLHCIKQ    GG++++ DGF     LK +NPQA+ +L++T ++F D G DY  F+  +  +I+  D  G++ R+N++N  R T  D+P++ V+PFY A++E+ D+++S E     KM PGD+IT  N R++HGR + E G    RHL+  Y D D V SR+R+L+  +
Sbjct:    1 MHCAILKAEAVDGARLMQIFWHDGAESLYPAVWLRDNCQCSDCYLHSAKARKLLLEALDVNIRMDDLTFDQK-KVYITWPNGHYSEFEANWLKKRCFSQEARAGLQGELFLPECQYWGSELQ--LPTLNFEDVLNDDDHAYKWLSSLKKVGIVRLTGAADKRGEIIKLGKRIGFLYLTFYGHTWQVQDKIDANNVAYTTGKLSFHTDYPALHHPPGVQLLHCIKQ-TVTGGDSEIVDGFNVCQKLKEKNPQAFSILSSTFVDFTDIGVDYCDFSVQSKHKIIELDDKGQVVRINFNNATRDTVFDVPIERVQPFYAALKEFVDLMNSKEYKYTFKMNPGDVITFDNWRLLHGRRSYEAGTEISRHLEGAYADWDVVMSRLRILRQRV 383          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. SwissProt
Match: gi|21541970|sp|Q924Y0.1|BODG_MOUSE (RecName: Full=Gamma-butyrobetaine dioxygenase; AltName: Full=Gamma-butyrobetaine hydroxylase; Short=Gamma-BBH; AltName: Full=Gamma-butyrobetaine,2-oxoglutarate dioxygenase)

HSP 1 Score: 234.572 bits (597), Expect = 2.375e-72
Identity = 134/391 (34.27%), Postives = 212/391 (54.22%), Query Frame = 0

Query:   20 LHRRFLGTSINRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKFTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQV---ELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALE-GKLTGRHLQCGYIDLDEVDSRIRVLQSEISD 406
            +H   L         ++Q  + DG +  +P VWLRD+CQ S  +  S+ +R   L    ++I+      + K K  ITW + H S F   +L+   F  +ARA  R++ ++   E + W  +     P  +F  V+        WL  L K G V L     + G I ++ +R+ F  LT YG  + V  +   +N+AYT   + FHTD     + PGV LLHCIKQ    GG++++ DGF     LK +NPQA+ +L++T ++F D G DY  F+  +  +I+  D  G++ R+N++N  R T  D+P++ V+PFY A++E+ D+++S E     KM PGD+IT  N R++HGR + E G    RHL+  Y D D V SR+R+L+  +++
Sbjct:    1 MHCAILKAEAVDGAHLMQIFWHDGAESLYPAVWLRDNCQCSDCYLHSAKARKLLLEALDVNIRIDDLTFDRK-KVYITWPNDHYSEFEANWLKKRCFSQQARA--RLQGELFLPECQYWGSELQ--LPTLNFEDVLNDDDHAYKWLSSLKKVGIVRLTGAADKRGEIIKLGKRIGFLYLTFYGHTWQVQDKIDANNVAYTTGKLSFHTDYPALHHPPGVQLLHCIKQ-TVTGGDSEIVDGFNVCQKLKEKNPQAFHILSSTFVDFTDIGVDYCDFSVQSKHKIIELDDKGQVVRVNFNNATRDTVFDVPIERVQPFYAALKEFVDLMNSKEYKYTFKMNPGDVITFDNWRLLHGRRSYEAGTEISRHLEGAYADWDVVMSRLRILRQRVTN 385          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. SwissProt
Match: gi|5915798|sp|O75936.1|BODG_HUMAN (RecName: Full=Gamma-butyrobetaine dioxygenase; AltName: Full=Gamma-butyrobetaine hydroxylase; Short=Gamma-BBH; AltName: Full=Gamma-butyrobetaine,2-oxoglutarate dioxygenase)

HSP 1 Score: 228.794 bits (582), Expect = 3.978e-70
Identity = 131/376 (34.84%), Postives = 205/376 (54.52%), Query Frame = 0

Query:   35 VVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSR--LTNLRDFAMDIQPVSCEHNSKGKFTITWEDGHVSPFSEEFLRNHSFQNEARAS-RRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALE-GKLTGRHLQCGYIDLDEVDSRIRVLQSEISD 406
            ++Q  + D  +  +P VWLRD+C  S  +  S+ +R  L    D  + I+ +  +   + K  ITW D H S F  ++L+   F  +ARA  +R     E + W  +     P  DF  V+   +    WL  L K G V L     + G + ++ +R+ F  LT YG  + V  +   +N+AYT   + FHTD     + PGV LLHCIKQ    GG++++ DGF     LK  NPQA+ +L++T ++F D G DY  F+  +  +I+  D  G++ R+N++N  R T  D+P++ V+PFY A++E+ D+++S E     KM PGD+IT  N R++HGR + E G    RHL+  Y D D V SR+R+L+  + +
Sbjct:   16 LMQILWYDEEESLYPAVWLRDNCPCSDCYLDSAKARKLLVEALDVNIGIKGLIFD---RKKVYITWPDEHYSEFQADWLKKRCFSKQARAKLQRELFFPECQYWGSELQ--LPTLDFEDVLRYDEHAYKWLSTLKKVGIVRLTGASDKPGEVSKLGKRMGFLYLTFYGHTWQVQDKIDANNVAYTTGKLSFHTDYPALHHPPGVQLLHCIKQ-TVTGGDSEIVDGFNVCQKLKKNNPQAFQILSSTFVDFTDIGVDYCDFSVQSKHKIIELDDKGQVVRINFNNATRDTIFDVPVERVQPFYAALKEFVDLMNSKESKFTFKMNPGDVITFDNWRLLHGRRSYEAGTEISRHLEGAYADWDVVMSRLRILRQRVEN 385          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. SwissProt
Match: gi|75041100|sp|Q5R5D8.1|BODG_PONAB (RecName: Full=Gamma-butyrobetaine dioxygenase; AltName: Full=Gamma-butyrobetaine hydroxylase; Short=Gamma-BBH; AltName: Full=Gamma-butyrobetaine,2-oxoglutarate dioxygenase)

HSP 1 Score: 228.024 bits (580), Expect = 6.721e-70
Identity = 130/377 (34.48%), Postives = 206/377 (54.64%), Query Frame = 0

Query:   34 QVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSR--LTNLRDFAMDIQPVSCEHNSKGKFTITWEDGHVSPFSEEFLRNHSFQNEARAS-RRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALE-GKLTGRHLQCGYIDLDEVDSRIRVLQSEISD 406
            +++Q  + D  +  +P VWLRD+C  S  +  S+ +R  L    D  + I+ ++ +   + K  ITW D H S F  ++L+      +ARA  +R     E + W  +     P  DF  V+   +    WL  L K G V L     + G + ++ +R+ F  LT YG  + V  +   +N+AYT   + FHTD     + PGV LLHCIKQ    GG++++ DGF     LK  NPQA+ +L++T ++F D G DY  F+  +  +I+  D  G++ R+N++N  R T  D+P++ V+PFY A++E+ D+++S E     KM PGD+IT  N R++HGR + E G    RHL+  Y D D V SR+R+L+  + +
Sbjct:   15 RLMQILWYDEEESLYPAVWLRDNCPCSDCYLDSAKARKLLAEALDVNIGIKGLTFD---RKKVYITWPDEHYSEFQADWLKKRCLSKQARAKLQRELFFPECQYWGSELQ--LPTLDFEDVLRYDEHAYKWLSTLKKVGIVRLTGASDKPGEVSKLGKRMGFLYLTFYGHTWQVQDKIDANNVAYTTGKLSFHTDYPALHHPPGVQLLHCIKQ-TVTGGDSEIVDGFNVCQKLKKNNPQAFQILSSTFVDFTDIGVDYCDFSVQSKHKIIELDDKGQVVRINFNNATRDTIFDVPVERVQPFYAALKEFVDLMNSKESKFTFKMNPGDVITFDNWRLLHGRRSYEAGTEISRHLEGAYADWDVVMSRLRILRQRVEN 385          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. SwissProt
Match: gi|300669619|sp|A5DCB6.2|TMLH_PICGU (RecName: Full=Trimethyllysine dioxygenase; AltName: Full=Epsilon-trimethyllysine 2-oxoglutarate dioxygenase; AltName: Full=TML hydroxylase; AltName: Full=TML-alpha-ketoglutarate dioxygenase; Short=TML dioxygenase; Short=TMLD)

HSP 1 Score: 158.688 bits (400), Expect = 2.706e-43
Identity = 108/382 (28.27%), Postives = 180/382 (47.12%), Query Frame = 0

Query:   32 DKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKFTITWE-DGHVSPFSEEFLRNHSFQ-NEARASRRMRDQVEL---ELWS-DDFHQNFPRADFRRVMTC----RKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHT--ELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCG-YIDLDEVDSRIRVL 400
            D   V  E+  G    F  +WLRD+C  S+ +  ++  RL N      DI P+  + +S  K  I W  + H S +   +L  HS+   +   + ++  + E+   E W+  D     P  DF+ VM       +    W   + K GF  + NVP++    +++ E++ + + THYG  +      + ++ AYT   I  HTD TY+  TPG+ L H +  HEG GG T L D F  A +LK  +P++++LL    +     G + +         I   D  G L ++ W+   R T    + P + V+ FY A++++  I+      L ++++PG  +   N R  H RT    + TG+   CG YI+ D+  SR+ +L
Sbjct:   14 DGDAVSVEWDGGASAKFDNIWLRDNCHCSECYYDATKQRLLNSCSIPDDIAPIKVD-SSPTKLKIVWNHEEHQSEYECRWLVIHSYNPRQIPVTEKVSGEREILAREYWTVKDMEGRLPSVDFKTVMASTDENEEPIKDWCLKIWKHGFCFIDNVPVDPQETEKLCEKLMYIRPTHYGGFWDFTSDLSKNDTAYTNIDISSHTDGTYWSDTPGLQLFHLL-MHEGTGGTTSLVDAFHCAEILKKEHPESFELLTRIPVPAHSAGEEKVCIQPDIPQPIFKLDTNGELIQVRWNQSDRSTMDSWENPSEVVK-FYRAIKQWHKIISDPANELFYQLRPGQCLIFDNWRCFHSRT----EFTGKRRMCGAYINRDDFVSRLNLL 388          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. SwissProt
Match: gi|118572730|sp|Q91ZW6.2|TMLH_RAT (RecName: Full=Trimethyllysine dioxygenase, mitochondrial; AltName: Full=Epsilon-trimethyllysine 2-oxoglutarate dioxygenase; AltName: Full=TML hydroxylase; AltName: Full=TML-alpha-ketoglutarate dioxygenase; Short=TML dioxygenase; Short=TMLD; Flags: Precursor)

HSP 1 Score: 155.992 bits (393), Expect = 4.371e-42
Identity = 103/358 (28.77%), Postives = 180/358 (50.28%), Query Frame = 0

Query:   48 FPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKFTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQN--FPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLN--FRDNGTDYIKFNKLNITRILS-HDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVL 400
            F +VWLRD C+S+  +++ +  R  +     + I+P +   +    F  TW DGHV+ +  ++L  +S++ + +   + R      LW+   +Q+   P  DF+  +  ++    +LQ+   +G   ++NVP  + H +++A RV+  + T YG  +      +  + AYT   +  HTD TY++   G+ + HC+K HEG GG T L DGF AA  +  R P+ +DLL+   L   + +N       + + +  IL+ +     L  + ++N+ R     +P D VR +Y A R     L   E  L  K+KPG ++ + N R++HGR +  G    R L   Y+  D+V +  R+L
Sbjct:   71 FDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTIRLDESTLF-FTWPDGHVTRYDLDWLVKNSYEGQKQEVIQPR-----VLWNAKLYQDAQLPSVDFQGFLETKEGLKKFLQNFLLYGIAFVENVPPTQEHTEKLARRVSLIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLK-HEGTGGRTLLVDGFYAAQQVLQRAPEEFDLLSQVPLKHEYIEN-VGQCHNHMIGVGPILNIYPWNKELYLIRYNNYDRAVINTVPYDVVRRWYAAHRTLTTELRRPENELWVKLKPGKVLFIDNWRVLHGRESFTGY---RQLCGCYLTRDDVLNTARIL 417          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. SwissProt
Match: gi|21542284|sp|Q91ZE0.2|TMLH_MOUSE (RecName: Full=Trimethyllysine dioxygenase, mitochondrial; AltName: Full=Epsilon-trimethyllysine 2-oxoglutarate dioxygenase; AltName: Full=TML hydroxylase; AltName: Full=TML-alpha-ketoglutarate dioxygenase; Short=TML dioxygenase; Short=TMLD; Flags: Precursor)

HSP 1 Score: 153.295 bits (386), Expect = 4.780e-41
Identity = 103/363 (28.37%), Postives = 180/363 (49.59%), Query Frame = 0

Query:   43 GTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKFTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQN--FPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLN--FRDNGTDYIKFNKLNITRILS-HDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVL 400
            GT   F +VWLRD C+S+  +++ +  R  +     + I+P +  H  +     TW DGHV+ +  ++L  +S++ + +   + R      LW+   +Q    P  DF+  +   +    +LQ+   +G   ++NVP  E H +++AER++  + T YG  +      +  + AYT   +  HTD TY++   G+ + HC+K HEG GG T L DGF AA  +  + P+ ++LL+   L   + +N       + + +  IL+ +     L  + ++N+ R     +P D V  +Y A R     L   E  L  K+KPG ++ + N R++HGR +  G    R L   Y+  D+V +  R+L
Sbjct:   66 GTVMRFDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTV-HLDETMLFFTWPDGHVTRYDLDWLVKNSYEGQKQKVIQPRI-----LWNSKLYQQAQVPSVDFQCFLETNEGLKKFLQNFLLYGIAFVENVPPTEEHTEKLAERISLIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLK-HEGTGGRTLLVDGFYAAQQVLQKAPEEFELLSKVPLKHEYIEN-VGQCHNHMIGVGPILNIYPWNKELYLIRYNNYDRAVINTVPYDVVHRWYTAHRTLTTELRRPENELWVKLKPGKVLFIDNWRVLHGRESFTGY---RQLCGCYLTRDDVLNTARLL 417          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. SwissProt
Match: gi|231642|sp|P80193.1|BODG_PSESK (RecName: Full=Gamma-butyrobetaine dioxygenase; AltName: Full=Gamma-butyrobetaine hydroxylase; Short=Gamma-BBH; AltName: Full=Gamma-butyrobetaine,2-oxoglutarate dioxygenase)

HSP 1 Score: 151.754 bits (382), Expect = 7.678e-41
Identity = 100/368 (27.17%), Postives = 164/368 (44.57%), Query Frame = 0

Query:   36 VQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKFTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFR--DNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVLQ 401
            V   + DG    F  +WLRD+C         +  ++  + D   DIQ  +      G+  + W+DGH S +   +LR H++  ++ A R    +     W      + P  D   VM    + L WL  +   G   L  VP E G +  +A+R++F + +++G  + V  +    + AYT   +  HTDL   +  PG+  LHC+  ++  GG +   DGF  A  L+   P AY LL  T + FR  D  +DY          +++ D +G +  +  +NF R     +    +  +Y A R +  +          +++ G +    N R++H R A +     RH Q  Y+D DE+ SRI VLQ
Sbjct:   25 VSVTWADGRVSPFHNLWLRDNCPCGDCVYEVTREQVFLVADVPEDIQVQAVTIGDDGRLVVQWDDGHASAYHPGWLRAHAYDAQSLAEREAA-RPHKHRWMQGL--SLPVYDHGAVMQDDDTLLEWLLAVRDVGLTQLHGVPTEPGALIPLAKRISFIRESNFGVLFDVRSKADADSNAYTAFNLPLHTDLPTRELQPGLQFLHCLV-NDATGGNSTFVDGFAIAEALRIEAPAAYRLLCETPVEFRNKDRHSDY-----RCTAPVIALDSSGEVREIRLANFLR-APFQMDAQRMPDYYLAYRRFIQMTREPRFCFTRRLEAGQLWCFDNRRVLHARDAFDPASGDRHFQGCYVDRDELLSRILVLQ 382          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. SwissProt
Match: gi|118574154|sp|Q0VC74.1|TMLH_BOVIN (RecName: Full=Trimethyllysine dioxygenase, mitochondrial; AltName: Full=Epsilon-trimethyllysine 2-oxoglutarate dioxygenase; AltName: Full=TML hydroxylase; AltName: Full=TML-alpha-ketoglutarate dioxygenase; Short=TML dioxygenase; Short=TMLD; Flags: Precursor)

HSP 1 Score: 152.14 bits (383), Expect = 1.078e-40
Identity = 99/358 (27.65%), Postives = 181/358 (50.56%), Query Frame = 0

Query:   48 FPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKFTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQN--FPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLN--FRDNGTDYIKFNKLNITRILS-HDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVL 400
            F +VWLRD C+S+  +++ +  R  +     + IQP +  H  +     TW DGHV+ +  ++L  +S++ + +   + R      LW+ + +Q    P  DF+  +  ++    +LQ+   +G   ++NVP  + H +++AER++  + T YG  +      +  + AYT   +  HTD TY++   G+ + HC+K HEG GG T L DGF AA  +  + P+ ++LL+   L   + +N  +  + + + +  +L+ +     L  + ++N+ R     +P D V  +Y A R     L   E     K+KPG ++ + N R++HGR +  G    R L   Y+  D+V +  R+L
Sbjct:   71 FDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIQPQTI-HLDETTLFFTWPDGHVTRYDLDWLMKNSYEGQKQKVIQPRI-----LWNAEIYQQAQVPAVDFQTFLETKEGLKNFLQNFLLYGIAFVENVPPTQKHTEKLAERISLIRETIYGRMWFFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLK-HEGTGGRTLLVDGFYAAEQVLQKAPEEFELLSKVPLKHEYIENVGE-CQNHMIGVGPVLNIYPWNKELYLIRYNNYDRAVINTVPYDVVHRWYTAHRTLTRELRRPENEFWVKLKPGKVLFIDNWRVLHGRESFTGY---RQLCGCYLTRDDVLNTARLL 417          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. SwissProt
Match: gi|21541975|sp|Q98KK0.1|BODG_RHILO (RecName: Full=Probable gamma-butyrobetaine dioxygenase; AltName: Full=Gamma-butyrobetaine hydroxylase; Short=Gamma-BBH; AltName: Full=Gamma-butyrobetaine,2-oxoglutarate dioxygenase)

HSP 1 Score: 146.747 bits (369), Expect = 6.585e-39
Identity = 95/377 (25.20%), Postives = 179/377 (47.48%), Query Frame = 0

Query:   29 INRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKFTITW-EDGHVSPFSEEFLRNHSF-QNEARASRRMRDQVELELWSDDFHQN-FPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTG-RHLQCGYIDLDEVDSRIRVLQ 401
            I  + + ++  +  G +  F  +WLRD+    K+  A +  RL  + D   + + +       G   +++  +G    F  ++L  +++ ++E R      D ++   W+    QN  PRA ++     R     WL  +  +GF ++  +P E G + ++++   + + T+YG  + V     P+N+AYT   +  HTD  Y    P + +L C+ ++   GGE+ + DGF  A  L+A NP+ + LL++    F   G+  ++         L  D  G L  + ++N +    +D+P  D+  +Y A R + +++   +  +  K++PG    V NTR++H R A  G  TG R LQ  Y D D + S +  ++
Sbjct:    7 IGDEGRTIELGWQGGRRTRFHAMWLRDNALDDKTRSAGNGQRLITILDIPAETR-IGAAEIKGGALEVSFVPEGKTVSFPAQWLSANAYDRDELREPGWTGDVIQR--WTKATMQNSVPRASYKAAFHGRSVLREWLSAVRTYGFAVMDGLPAESGALCKVSDLFGYIRETNYGRWFEVRAEVNPNNLAYTNLGLQAHTDNPYRDPVPTLQILACV-ENTVEGGESSVIDGFAVAAALQAENPEGFRLLSSCPARFEYAGSSGVRLQAKRPMIELGPD--GELICIRFNNRSLAPVVDVPFADMDAYYAAYRRFAELIEDPDFEVTFKLQPGQAFIVDNTRVMHARKAFSG--TGKRWLQGCYADKDGLLSTLAAIE 375          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. nr
Match: gi|260810424|ref|XP_002599964.1| (hypothetical protein BRAFLDRAFT_212201 [Branchiostoma floridae] >gi|229285248|gb|EEN55976.1| hypothetical protein BRAFLDRAFT_212201 [Branchiostoma floridae])

HSP 1 Score: 308.916 bits (790), Expect = 7.192e-99
Identity = 152/379 (40.11%), Postives = 224/379 (59.10%), Query Frame = 0

Query:   27 TSINRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKFTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVLQSEIS 405
              +N   +VV+ E++ G    FP+VWLRD+CQ  K F+  S +RL  + D  + + PV  E  +    T+ W DGH S +   +L+   F  +A   R    Q + +LW  +  Q+ P+ADF  ++T  ++   +L  L   G VL+QNVP E G + ++A RVAF K T+YG  + V  +  PSN+AYT   +G HTDL  Y YTPGV +LHCI+Q +G GG+  L DG   A+ LK  NP+A+ LL    +NF+D+G DY +F+      ILS D  G +E +N+++  R + LDLP + V+P YDA++ Y  I++     ++HKM  G+MI   NTR +HGR A       RHLQ GY+D DE+ SR+RVL++++ 
Sbjct:    4 VCLNEAARVVEVEWSGGGVSRFPYVWLRDNCQCQKCFNPDSRARLVLMADLDVKLSPVRAEVQAGSLLTVDWPDGHQSQYDWPWLKARCFSTQALEKRGKDWQRKTQLWGSELAQDLPKADFSVLLTDDRALYDFLFLLDSVGLVLVQNVPCEVGQVDRLANRVAFLKHTNYGLEFVVKSKPNPSNVAYTSAKLGLHTDLPQYNYTPGVQMLHCIEQSKGEGGDNHLVDGINVAYQLKEENPEAFRLLTTLKVNFKDDGLDYHRFDLRERRTILSLDDQGNVESINYNDQVRDSILDLPAEQVQPLYDALKAYNTIMYLPRNCVRHKMAAGEMIAFNNTRTLHGRLAYSLTGGTRHLQGGYLDWDEIYSRMRVLKTDLG 382          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. nr
Match: gi|1126177104|ref|XP_019620852.1| (PREDICTED: gamma-butyrobetaine dioxygenase-like [Branchiostoma belcheri])

HSP 1 Score: 301.212 bits (770), Expect = 9.235e-96
Identity = 152/378 (40.21%), Postives = 225/378 (59.52%), Query Frame = 0

Query:   29 INRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKF-TITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVLQSEIS 405
            +N   +VV+ E++ G    FP+VWLRD+CQ  + F+  S  RL  + D  + I PV     + G   T+ W DGH S +   +L+   F   A   R    Q +  LW  +   +FP ADF  ++T  ++   +L  L   G VL+QNVP E G + ++A RVAF KLT+YG  + V+ +  PSN+AYT   +G HTDL YY YTPGV +L CI+Q +G GG+  L DGF AA+ LK  NP+A+ LL    +NF++ GTDY +F       I+S D  G+++ +N+++ AR + LDL  D V+  YDA++ Y  I++  +  ++HKM+ G+M+   NTR +HGR A       RHLQ GY+D DE+ SR+RVL++++ 
Sbjct:    6 LNEAGRVVEVEWSGGGVSRFPYVWLRDNCQCQQCFNPDSHGRLLLMADLDVRISPVRAAVQAAGSLLTVDWPDGHQSQYDWPWLKARCFSTPALEKRAGDWQRKTRLWGAELADDFPTADFPALLTDDRALYDFLFLLDSVGLVLVQNVPCEVGQLDRLANRVAFLKLTNYGKEFVVMSKPNPSNVAYTSVKLGLHTDLPYYNYTPGVQMLQCIEQCKGEGGDNYLVDGFNAAYQLKEENPEAFRLLTTVKVNFQNKGTDYHRFFLRERRTIISLDDQGKVKSVNFNDPARDSILDLREDQVQSLYDALKAYNTIMYLPKNCVRHKMEAGEMLAFNNTRTLHGRIAFSQTSGSRHLQGGYLDWDEIHSRMRVLKTDLG 383          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. nr
Match: gi|1126177689|ref|XP_019621167.1| (PREDICTED: gamma-butyrobetaine dioxygenase-like [Branchiostoma belcheri])

HSP 1 Score: 295.049 bits (754), Expect = 2.040e-93
Identity = 148/380 (38.95%), Postives = 223/380 (58.68%), Query Frame = 0

Query:   27 TSINRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKF-TITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVLQSEIS 405
             ++N   ++V+ E++DG    FP+VWLRD+CQ  + F+  S SR   + D  +++ PV  E  +KG   ++ W  GH S +   +LR   F  +A + R    Q   +LW  +   N P+ADF  ++T  ++   +L  L   G VL+QNVP + G ++++A RVAF K T YG  + V+ +  P+++AYT   IG HTDL YY Y PGV +LHCI+Q  G GG+  L DG  AA+ LK  NP A+ LL    +NF   G D  KF       ++S +  G ++ +++S+ AR + LDLP D V+PFYDA++ ++ I++     L+ KM  G+MI   NTR +HGR+A     + RHLQ GY D DE+ SR+RVL+ ++ 
Sbjct:    4 AALNEAARMVEVEWSDGGVSRFPYVWLRDNCQCPQCFNPDSLSRRVLMNDLDVNVSPVRVELQAKGSLLSVEWPQGHESQYDCHWLRERCFSPQALSERERDWQKRTQLWGAELAHNLPKADFPAILTDDRALHDFLVVLDSVGLVLVQNVPCDVGQVERLANRVAFLKPTSYGLEFEVVKKPKPNDLAYTTVKIGLHTDLNYYSYRPGVQMLHCIEQTRGEGGDNSLTDGINAAYQLKKENPDAFRLLTTLKVNFYCTGVDLYKFFFRQRRNVISLNDQGEVQSVSFSDQARDSILDLPADQVQPFYDALKAFDTIMYLPRNCLRFKMAAGEMIAFDNTRSLHGRSAYSLVDSTRHLQGGYFDWDEIYSRMRVLKVDLG 383          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. nr
Match: gi|1126187605|ref|XP_019626570.1| (PREDICTED: gamma-butyrobetaine dioxygenase-like [Branchiostoma belcheri])

HSP 1 Score: 286.96 bits (733), Expect = 3.246e-90
Identity = 145/380 (38.16%), Postives = 225/380 (59.21%), Query Frame = 0

Query:   27 TSINRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKF-TITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVLQSEIS 405
             ++N   ++V+ E++D     FP+VWLRD+CQ  + F+  S SR   + D  +++ PV  E  +K    ++ W  GH S +  ++LR   F  +A + R    Q + +LW  +   N P+ADF  ++T  +    +L  L   G VLLQNVP + G ++++A RVAF K T +G  + V+ +  P+++AYT   IG HTDL YY+Y PGV +LHCI+Q  G GG+  L DG  AA+ L+  NP A+ LL    +NF   G D  KF       ++S +  G ++ +++S+ AR + LDLP D V+PFYDA++ ++ I++     ++ KM  G+MI   NTR +HGR+A     T RHLQ GY+D DE++SR+RVL+ ++ 
Sbjct:    4 VALNEAARMVEVEWSDRGVSRFPYVWLRDNCQCPQCFNPDSRSRRVLMNDLDVNVSPVRVELQAKKSLLSVDWPQGHQSQYDCQWLRERCFSPQALSERERDWQRKTQLWGAELAHNLPKADFPAILTDDRVLHDFLVVLDSVGLVLLQNVPCDVGQVERLANRVAFLKPTSFGLEFEVVNKPKPNDLAYTTVKIGLHTDLNYYRYRPGVQMLHCIEQTRGEGGDNSLTDGINAAYQLQEENPDAFRLLTTLKVNFYCTGVDLYKFFFRQRRNVISLNDQGEVQSVSFSDQARDSILDLPADQVQPFYDALKAFDTIMYLPRNCIRFKMAAGEMIVFDNTRTLHGRSAYSLDST-RHLQGGYLDWDEINSRMRVLKVDLG 382          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. nr
Match: gi|1126208510|ref|XP_019637922.1| (PREDICTED: gamma-butyrobetaine dioxygenase-like [Branchiostoma belcheri])

HSP 1 Score: 276.944 bits (707), Expect = 1.453e-86
Identity = 146/380 (38.42%), Postives = 213/380 (56.05%), Query Frame = 0

Query:   27 TSINRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKF-TITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVLQSEIS 405
             S+N   +VV+ E++DG    FP+VWLRD+CQ  + F+  S +RL  + D  + + PV  E  + G   T+ W DGH S +   +L+   F   A   R    Q +  LW  +   + P ADF  ++T  ++   +L  L   G VL+QNVP + G + ++A RVAF KLT+YG                    +G HTDL  Y YTPGV +LHCI+Q +G GG+  L DGF  A+ LK  NP+A+ LL    +NF+D G DY +F       I+S D  G ++ +N+++  R + LDLP D V+  YDA++ Y  IL+  +  ++HKM  G+MI   NTR +HGR A       RHLQ GY+D DE+ SR+RVL++++ 
Sbjct:    4 VSLNGAGRVVEVEWSDGGVSRFPYVWLRDNCQCQQCFNPDSRARLVLMADLDVRLSPVRAEVQAAGSLLTVDWPDGHQSQYDWPWLKARCFSTPALEKRAGDWQRKTRLWGAELADDLPTADFPALLTDDRALYDFLFLLDSVGLVLVQNVPCDVGQLDRLANRVAFLKLTNYGV------------------KLGLHTDLPQYNYTPGVQMLHCIEQCKGEGGDNHLVDGFNVAYQLKQENPEAFRLLTTLKVNFQDEGMDYHRFFLREKHTIISLDDQGNVQAINYNDQIRDSILDLPADQVQSLYDALKAYNTILYLPKNCVRHKMAAGEMIAFNNTRTLHGRLAFSQTGGSRHLQGGYLDWDEIYSRMRVLKTDLG 365          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. nr
Match: gi|1126169544|ref|XP_019616725.1| (PREDICTED: gamma-butyrobetaine dioxygenase-like isoform X1 [Branchiostoma belcheri])

HSP 1 Score: 280.026 bits (715), Expect = 1.820e-86
Identity = 141/380 (37.11%), Postives = 224/380 (58.95%), Query Frame = 0

Query:   27 TSINRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKF-TITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVLQSEIS 405
             ++N   ++V+ E++DG    FP+VWLRD+CQ    F+  S SR   + D  +++ PV  +  + G   ++ W  GH S +  ++LR   F  +AR+ R    Q + +LW  +   N P+ADF  ++T  ++   +L  L   G VL+Q VP + G ++++A RVA+ + T++G  + V+ +  PS++AYT   +G HTDL +Y + PGV +LHCI+Q    GGE+ L DGF  A+ LK  NP A+ LL    +NF + G D+ KF        +S +  G ++ +++S+ AR + LDLP D V+PFYDA++ ++ I++     L+ KM  G+MI   NTR +HGR+A       RHLQ GY D DE+ SR+RVL+ ++ 
Sbjct:   94 VALNGAARMVEVEWSDGGVSRFPYVWLRDNCQCPLCFNPDSLSRRVLMNDLDVNVSPVRVKLQANGSLLSVEWPQGHQSQYDWQWLRERCFSPQARSEREKDWQRKTQLWGAELVHNLPKADFPAILTHDRALHDFLALLDSVGLVLVQKVPCDVGQVERLANRVAYLRPTNFGKEFVVVNKPNPSDLAYTTGKLGLHTDLNFYDHKPGVQMLHCIEQTMREGGESHLVDGFSVAYQLKEENPDAFRLLTTLKVNFCNVGLDFHKFFLRQRQHTISLNDQGEVQSVSFSDQARDSILDLPADQVQPFYDALKAFDTIMYLPRNCLRFKMAAGEMIVFDNTRTLHGRSAYSLDDI-RHLQGGYFDWDEIYSRMRVLKIDLG 472          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. nr
Match: gi|1126169821|ref|XP_019616875.1| (PREDICTED: gamma-butyrobetaine dioxygenase-like [Branchiostoma belcheri])

HSP 1 Score: 275.018 bits (702), Expect = 1.561e-85
Identity = 144/381 (37.80%), Postives = 213/381 (55.91%), Query Frame = 0

Query:   27 TSINRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGK-FTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDIL-HSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVLQSEIS 405
              ++R  + V+ E++ G  D FP+VWLRD+CQ S+ F      RL       +D+ P       +G    +TW DGH S +  ++LR+  F  +ARA R  R + + +LW  +   N P ADF  +++  ++   +L  L   G VL+QNVP + G   ++A RVAF K+T  G+   + ++    N   +   +G HTDL YY Y PGV ++HCIKQ E  GG ++L D F AA+ LK  NP A+ LL    +NF   G    + +      I+S    G ++++     +R + LD+PLD V+PFY AMR ++DIL H   C +++KMK GDM+   N R++H RTA       RHLQ GY+D DE  SR+RVLQ+E+ 
Sbjct:    4 AKLDRSSETVEVEWSAGGVDWFPYVWLRDNCQCSECFQVDLNKRLVLTSRLDLDVAPDHANVQDRGSTLHVTWPDGHRSEYGWQWLRDRCFSPQARADRETRWRRKKQLWGAELFTNLPTADFPALLSDDRALYDFLFLLDTIGLVLVQNVPRQPGQFLKLANRVAFPKMTFLGAEQMLKIQPIHGNFLLSTDPLGLHTDLAYYNYLPGVQMMHCIKQTESEGGASELVDAFNAAYQLKQENPDAFKLLTAVKVNFHRIGKAEPRHHMKERHHIISVSDKGEVQKVVCGKHSRDSVLDVPLDHVKPFYRAMRAFDDILAHPRNC-IRYKMKEGDMLAFDNLRVLHDRTAFSLSGGERHLQVGYVDWDETYSRMRVLQAELG 383          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. nr
Match: gi|260805254|ref|XP_002597502.1| (hypothetical protein BRAFLDRAFT_219801 [Branchiostoma floridae] >gi|229282767|gb|EEN53514.1| hypothetical protein BRAFLDRAFT_219801 [Branchiostoma floridae])

HSP 1 Score: 271.937 bits (694), Expect = 2.174e-84
Identity = 143/380 (37.63%), Postives = 213/380 (56.05%), Query Frame = 0

Query:   27 TSINRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKG-KFTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQVELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALEGKLTGRHLQCGYIDLDEVDSRIRVLQSEIS 405
              ++   + V+ E++ G  D FP++WLRD+CQ S+ F A    RL    +  +D+ P       +G    +TW DGH S +  ++LR+  F  +ARA R  R + + +LW  +   N P ADF  ++T  ++   +L  L   G VL+QNVP + G  +++A RVAF K+T  G+   + V+    N   +   +G HTDL YY Y PGV ++HCIKQ E  GG ++L D F AA+ LK  NP A+ LL    +NF   G    K +      I+S    G ++++     +R + LD+P+D V+PFY AMR ++ IL +    +++KMK GDM+   N RI+H RTA       RHLQ GY+D DE  SR+RVLQ+E+ 
Sbjct:    4 AKLDSSSETVEVEWSAGGVDRFPYIWLRDNCQCSECFQADLNKRLVLTSELDLDVAPAHAAARDRGNSLHVTWPDGHQSRYDWQWLRDRCFSTQARADRERRWRRKKQLWGAELFTNLPTADFPALLTDDRALYDFLFLLDTIGLVLVQNVPRQVGQFQELANRVAFPKMTFLGAEQMLKVKPIHGNFLLSTAPLGLHTDLAYYNYLPGVQMMHCIKQTESDGGASELVDAFNAAYQLKEENPDAFKLLTTVKVNFHRIGKAEPKHHMRERHHIISVSDQGEVQKVVCGKHSRDSVLDVPVDQVKPFYRAMRAFDTILSNPRNCIRYKMKEGDMLAFDNLRILHDRTAFSMSGGERHLQVGYVDWDETYSRMRVLQAELG 383          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. nr
Match: gi|291234065|ref|XP_002736971.1| (PREDICTED: gamma-butyrobetaine dioxygenase-like [Saccoglossus kowalevskii])

HSP 1 Score: 269.626 bits (688), Expect = 1.742e-83
Identity = 137/386 (35.49%), Postives = 221/386 (57.25%), Query Frame = 0

Query:   26 GTSINRDKQVVQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGK-FTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQV---ELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIK--FNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALE-GKLTGRHLQCGYIDLDEVDSRIRVLQSEI 404
             T ++ D+Q+    FTDG +  +P++WL D+C+ ++ +  S+  RL           P S      G   +I W  GH+  +S ++LR H F +         D+V   +L +W  +     P  DF  ++        WL  L+  G  L++NVP E G IK++A+RV+F K T+YG  ++V  +  PSN AYT   +  HTDL+ Y + PGV +LHCI+Q++G GG+++  DGFKAA  LK  NP+A+++L  T LNF D G DY    FN+ + T  L     G +E + +S  +  + + +P++ V   Y+A++ + DI+++ E  +K KM+ GDM+   N R++HGR+A +  + + RHL+ GYID DE+ SR+RVL++ +
Sbjct:    4 ATCVDEDRQIKAI-FTDGLQSKYPYIWLLDNCRCTQCYVHSANQRLVPFSQLDFRAVPESVTLTPDGSHLSIKWSKGHIGDYSVDWLRTHQFDSSKP------DEVAEPQLHIWGSEMVNTIPTYDFEGILNNEAELYKWLDSLNASGLALIKNVPQETGQIKRVAKRVSFLKPTNYGVCFSVKSKMNPSNTAYTAAGLSLHTDLSNYNHAPGVQMLHCIQQYDGEGGDSEFTDGFKAAIQLKKENPKAFEILTTTWLNFSDIGIDYYSYHFNRQSPTICLGRK--GNIEEIAYSRTSLSSMMYIPVEQVESVYEALKAFSDIMYNKENIIKFKMESGDMVAFNNIRVLHGRSAFKVEQRSSRHLEGGYIDWDEIHSRMRVLKANL 380          

BLAST of hypothetical protein BRAFLDRAFT_212201 vs. nr
Match: gi|663245451|ref|XP_008503082.1| (PREDICTED: gamma-butyrobetaine dioxygenase [Calypte anna] >gi|676767232|gb|KFO96844.1| Gamma-butyrobetaine dioxygenase [Calypte anna])

HSP 1 Score: 268.47 bits (685), Expect = 4.808e-83
Identity = 140/373 (37.53%), Postives = 215/373 (57.64%), Query Frame = 0

Query:   36 VQFEFTDGTKDDFPFVWLRDSCQSSKSFDASSFSRLTNLRDFAMDIQPVSCEHNSKGKFTITWEDGHVSPFSEEFLRNHSFQNEARASRRMRDQV---ELELWSDDFHQNFPRADFRRVMTCRKSFLAWLQDLSKFGFVLLQNVPLEEGHIKQIAERVAFEKLTHYGSGYTVIVRDTPSNIAYTPHTIGFHTDLTYYKYTPGVVLLHCIKQHEGRGGETQLADGFKAAHLLKARNPQAYDLLANTTLNFRDNGTDYIKFNKLNITRILSHDLTGRLERLNWSNFARHTELDLPLDDVRPFYDAMREYEDILHSNECHLKHKMKPGDMITVKNTRIVHGRTALE-GKLTGRHLQCGYIDLDEVDSRIRVLQSEI 404
            V  E+ DG++  +P VWLRDSC+  + F +S+ +R   L D  ++I         + K +I W D H S F  E+L+   F   ARA   MR+++   E E W  D     P+  F  +M   +S   WL  L K G VLL     ++G + ++ +R+ F +LT YG  + V  +   +N+AYT   + FHTD    ++ PGV  LHCIKQ    GGE+++ DGF  ++ LKA++PQAY +L +T +++ D G DY +F      RI+  D  G+  R+N++N  R +  D+P D VRPFY A++E++D+L+S E    +K+KPGD++T  N R++HGR + + G    RHL+  Y D D V SR+R+L+ +I
Sbjct:   15 VHVEWEDGSESRYPCVWLRDSCRCPRCFLSSAGARKLLLEDLDVNIVVKEVTLAGREKISIIWPDEHASEFEAEWLKKRCFSEAARAE--MREELFLTECEYWGSDLQ--LPKMPFEEIMHNDESTYKWLCTLKKVGIVLLTGAAAQQGELLRLGQRIGFLRLTFYGPTWQVQDKADANNVAYTTGRLCFHTDYPVLQHPPGVQFLHCIKQ-TAEGGESEVVDGFHVSNKLKAQSPQAYQILTSTAVDYTDVGVDYCEFAVQCKQRIIDVDSRGQAVRINYNNATRDSVFDVPADSVRPFYAALKEFDDLLNSAEHKFTYKLKPGDIVTFDNWRVLHGRRSFQSGSEVSRHLEGAYGDWDVVMSRLRLLRKKI 382