BLAST of fumarate hydratase class ii vs. L. salmonis genes
Match: EMLSAG00000011633 (supercontig:LSalAtl2s:LSalAtl2s806:113709:119311:1 gene:EMLSAG00000011633 transcript:EMLSAT00000011633 description:"maker-LSalAtl2s806-augustus-gene-0.29")

HSP 1 Score: 371.703 bits (953), Expect = 5.104e-122
Identity = 197/429 (45.92%), Postives = 270/429 (62.94%), Query Frame = 0

Query:    6 QRWGSETQKAIDNFPI---SGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGR-HDDQFPVDIFQTGSGTSTNMNANEVIADLA----------GDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGV 420
            + +G++T ++I NFPI     E +P  V+   G LK AAAEVN E G LD   A  I  AA EV  G  +++ FP+ I+QTGSGT +NMN NEVIA+ A             VHPNDHVN  QSSND +PTA+H+A      N+L P L  L  AL  KA E+ D  K GRTH  DA P+TLGQEF  Y  QI+ G +R++  + R+  +  GGTAVG+GLNT   FA  +  ++ E  G+   T  ++FEA A  D++VE+SGAL  IA S+ KIA D+ ++ASGPR GLGE++LP  + GSSIMPGKVNP   E +  VAAQV+GN  A+T+GG  G+FELNV  P++  N++ S+ ++  S   F + CV+GI  N +R+  L   +L   T LNP +GYD A +I + A ++G+++++ A+A G 
Sbjct:  323 KYYGAQTSRSIINFPIGDRQSERMPLPVIKGFGILKKAAAEVNKEYG-LDSKIADAISKAADEVISGDLYNEHFPLVIWQTGSGTQSNMNTNEVIANRAIQLLGGKMGSKSPVHPNDHVNKSQSSNDTYPTAMHIAVAVEVHNTLIPGLKKLHDALDAKANEYKDIIKIGRTHTQDATPLTLGQEFSAYVTQIKFGIERIETTLPRLYMLAAGGTAVGTGLNTRIGFAEKIAAKVSELTGLPFITAPNKFEALAAHDSMVEVSGALNVIACSIMKIANDLRFLASGPRCGLGELSLPENEPGSSIMPGKVNPTQCEAITMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMVANVLRSIRLIGDSCVAFTDNCVNGIEVNRERVDKLLHESLMLVTALNPHIGYDAAAKIAKTAHKNGTTLKDEAIALGY 750          

BLAST of fumarate hydratase class ii vs. SwissProt
Match: gi|39931630|sp|Q9FBN6.1|FUMC_STRCO (RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase)

HSP 1 Score: 449.899 bits (1156), Expect = 4.598e-155
Identity = 235/440 (53.41%), Postives = 306/440 (69.55%), Query Frame = 0

Query:    7 RWGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLA----GDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARG------VDAALLDQALDPLRIAR 436
            +W ++TQ+A++NFP+SG+ +    +  L R+K+AAA+VNAELG LD   A  I  AAGEVA+G+ D+ FPVD+FQTGSGTS+NMN NEV+A LA    G DVHPNDHVN  QSSNDVFP+++H+AA       L PAL  L  AL  KA EFAD  K+GRTHLMDA PVTLGQEFGGYAAQ+R G +RL A++ R+ ++PLGGTAVG+G+NT P F+  VIE +    G+ +    D FEAQ  RD +VE SG L+TI V LTKIA D+ WMASGPR GL EI+LP LQ GSSIMPGKVNPV+ E VL VAAQV GND  +   G  GNFELNV +P+IA+N+++S+ +L++   + A+R V GI A+ +R    A+++ +  T LN  +GY+EA ++ + A     ++R+  L  G      +    LDQALD LR+ R
Sbjct:   21 KWRAQTQRAVENFPVSGQRIERAHIEALARIKSAAAKVNAELGVLDEDVAGAIQEAAGEVAEGKWDEHFPVDVFQTGSGTSSNMNTNEVVATLATERLGRDVHPNDHVNASQSSNDVFPSSIHIAATAAVTRDLIPALDHLAGALERKAGEFADVVKSGRTHLMDATPVTLGQEFGGYAAQVRYGIERLQASLPRLAELPLGGTAVGTGINTPPGFSAAVIEEVARATGLPLTEARDHFEAQGARDGIVETSGQLRTIGVGLTKIANDLRWMASGPRTGLAEISLPDLQPGSSIMPGKVNPVIPEAVLMVAAQVTGNDATVAAAGAAGNFELNVMLPVIAKNVLESVRLLANVSRLLADRTVDGIVAHPERAREYAESSPSVVTPLNKYLGYEEAAKVAKRALAERKTIRQTVLEGGYVERGDLTREQLDQALDVLRMTR 460          

BLAST of fumarate hydratase class ii vs. SwissProt
Match: gi|39931596|sp|Q8NRN8.1|FUMC_CORGL (RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase)

HSP 1 Score: 439.884 bits (1130), Expect = 6.403e-151
Identity = 230/443 (51.92%), Postives = 305/443 (68.85%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLA---GDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVK-VETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARG-VDAAL-----LDQALDPLRIARGNQE 440
            W ++TQ+A++NFPISG  +    +  +G LKAA A+VN + G LD  +A  I+AA  E+A G+HD +FP+D+FQTGSGTS+NMN NEVIA +A   G +VHPNDHVN+GQSSND FPTA H+AA + A+N L P L  L  +LA KA E+++  K+GRTHLMDAVPVTLGQEFGGYA QI+LG +R++A + R+ ++ +GGTA G+G+NT  DF   V+  L     VK ++   + FEAQA RDALVE SGA++ IAVSL KIA DI  M SGP  GLGEI LP LQ GSSIMPGKVNPVL E   QV+AQVIGND A+   G QG FELNV +P++ARN+++S  +L+++  VFA R V GI  N   +  LA+++ +  T LN  +GY+ A ++ + A   G ++R+  +  G VD        LD+ LD L +A   +E
Sbjct:   28 WQAQTQRAVENFPISGRGLESAQIRAMGLLKAACAQVNKDSGALDAEKADAIIAAGKEIASGKHDAEFPIDVFQTGSGTSSNMNTNEVIASIAKANGVEVHPNDHVNMGQSSNDTFPTATHVAATEAAVNDLIPGLKVLHESLAKKANEWSEVVKSGRTHLMDAVPVTLGQEFGGYARQIQLGIERVEATLPRLGELAIGGTAAGTGINTSADFGGKVVAELINLTDVKELKEAENHFEAQAARDALVEFSGAMRVIAVSLYKIANDIRLMGSGPLTGLGEIRLPDLQPGSSIMPGKVNPVLCETATQVSAQVIGNDAAVAFSGTQGQFELNVFIPVMARNVLESARLLANTSRVFATRLVDGIEPNEAHMKELAESSPSIVTPLNSAIGYEAAAKVAKTALAEGKTIRQTVIDLGLVDGEKLTEEELDKRLDVLAMAHTERE 470          

BLAST of fumarate hydratase class ii vs. SwissProt
Match: gi|39931371|sp|Q7U0N6.1|FUMC_MYCBO (RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase >gi|613782868|sp|P9WN92.1|FUMC_MYCTO RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase >gi|613782877|sp|P9WN93.1|FUMC_MYCTU RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase)

HSP 1 Score: 431.409 bits (1108), Expect = 1.365e-147
Identity = 235/441 (53.29%), Postives = 309/441 (70.07%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLA---GDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFA-RLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARG-----VDAALLDQALDPLRIARGNQ 439
            W ++TQ+A++NFPISG  +    +  LG LK A A+VN++LG L   +A  I+AAA E+ADG+HDDQFP+D+FQTGSGTS+NMN NEVIA +A   G  +HPND VN+ QSSND FPTA H+AA + A+  L PAL  L  ALA KA ++    K+GRTHLMDAVPVTLGQEF GYA QI  G +R+ A + R+ ++ +GGTAVG+GLN   DF  R+V   + + G  ++ T  + FEAQA RD LVE SGAL+TIAVSLTKIA DI WM SGP  GL EI LP LQ GSSIMPGKVNPVL E V QVAAQVIGND AI  GG  G FELNV +P++ARN+++S  +L++   +FA+RC++G+TAN + L  LA+++ +  T LN  +GY+EA  + ++A +   ++R+  + RG     +    LD+ LD L +A+  Q
Sbjct:   28 WRAQTQRAVENFPISGRGLERTQIRALGLLKGACAQVNSDLGLLAPEKADAIIAAAAEIADGQHDDQFPIDVFQTGSGTSSNMNTNEVIASIAAKGGVTLHPNDDVNMSQSSNDTFPTATHIAATEAAVAHLIPALQQLHDALAAKALDWHTVVKSGRTHLMDAVPVTLGQEFSGYARQIEAGIERVRACLPRLGELAIGGTAVGTGLNAPDDFGVRVVAVLVAQTGLSELRTAANSFEAQAARDGLVEASGALRTIAVSLTKIANDIRWMGSGPLTGLAEIQLPDLQPGSSIMPGKVNPVLPEAVTQVAAQVIGNDAAIAWGGANGAFELNVYIPMMARNILESFKLLTNVSRLFAQRCIAGLTANVEHLRRLAESSPSIVTPLNSAIGYEEAAAVAKQALKERKTIRQTVIDRGLIGDRLSIEDLDRRLDVLAMAKAEQ 468          

BLAST of fumarate hydratase class ii vs. SwissProt
Match: gi|39931486|sp|Q82ID7.1|FUMC_STRAW (RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase)

HSP 1 Score: 427.172 bits (1097), Expect = 4.937e-146
Identity = 239/438 (54.57%), Postives = 306/438 (69.86%), Query Frame = 0

Query:    7 RWGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLA----GDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGV----DAAL--LDQALDPLRI 434
            +W ++TQ+A++NFPISG+ +    +  L R+K AAA+VNAELG LD+  A  I  AA EVA+GR D+ FPVD+FQTGSGTS+NMNANEVIA LA    G DVHPNDHVN  QSSNDVFP+++H+AA       L PAL  L  AL  K+ EFAD  KAGRTHLMDA PVTLGQEFGGYAAQ+R G +RL A++ R+ ++PLGGTAVG+G+NT P F+  VI  +    G+ +    D FEAQ  RD +VE SG L+TIAV LTKIA D+ WMASGPR GL EI+LP LQ GSSIMPGKVNPV+ E VL VAAQV GND  +   G  GNFELNV +P+IA+N+++S+ +L+    + A+R V GI A+ DR    A+++ +  T LN  +GY+EA ++ ++A     ++R+  L  G     D  L  LD+ALD LR+
Sbjct:   27 KWRAQTQRAVENFPISGQRLERAHIEALARIKGAAAKVNAELGVLDKDVAEAIQEAAAEVAEGRWDEHFPVDVFQTGSGTSSNMNANEVIATLATERLGRDVHPNDHVNASQSSNDVFPSSIHIAATAAVTRDLVPALEHLAAALGRKSEEFADVVKAGRTHLMDATPVTLGQEFGGYAAQVRYGVERLAASLPRLAELPLGGTAVGTGINTPPGFSAAVIAEVARVTGLPLTEARDHFEAQGARDGIVETSGQLRTIAVGLTKIANDLRWMASGPRTGLSEISLPDLQPGSSIMPGKVNPVIPEAVLMVAAQVTGNDATVAAAGAAGNFELNVMLPVIAKNVLESVRLLAHVSRLLADRTVDGIVAHRDRAREYAESSPSVVTPLNKYIGYEEAAKVAKKALAERQTIRQVVLESGYVDRGDLTLEQLDEALDVLRM 464          

BLAST of fumarate hydratase class ii vs. SwissProt
Match: gi|39931581|sp|Q8FQP8.2|FUMC_COREF (RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase)

HSP 1 Score: 425.631 bits (1093), Expect = 2.982e-145
Identity = 231/446 (51.79%), Postives = 309/446 (69.28%), Query Frame = 0

Query:    5 QQRWGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLA---GDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGV-KVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARG-VDAAL-----LDQALDPLRIARGNQE 440
            Q  W ++TQ+A++NFPISG  +    +  +G LKAA A+VN + G LD A+A  I+AA  E+A G+HD +FP+D+FQTGSGTS+NMN NEVIA +A   G +VHPND VN+GQSSND FPTA H+AA + A+N L P L  L  +LA KA E+    K+GRTHLMDAVPVTLGQEFGGYA QI+LG +R++A + R+ ++ +GGTAVG+G+NT  DF   V+  L E  GV +++   + FEAQA RDALVE SGA++ +AVSL KIA DI  M SGP  GLGEI LP LQ GSSIMPGKVNPVL E   QV+AQVIGND A+   G QG FELNV +P++ARN+++S  +L+++  VFA R V GI  N + +  LA+++ +  T LN  +GY+ A ++ + A   G ++R+  +  G VD        LD+ LD L +A  +++
Sbjct:   25 QALWRAQTQRAVENFPISGRGLESAQIRAMGLLKAACAQVNKDSGALDAAKADAIIAAGKEIATGKHDAEFPIDVFQTGSGTSSNMNTNEVIASIAKANGTEVHPNDDVNMGQSSNDTFPTATHVAATEAAVNDLIPGLKVLHESLAKKANEWDSVVKSGRTHLMDAVPVTLGQEFGGYARQIQLGIERIEATLPRLGELAIGGTAVGTGINTSADFGGKVVAELVELTGVTQLQEAANHFEAQANRDALVEFSGAMRVVAVSLYKIANDIRLMGSGPLTGLGEIQLPDLQPGSSIMPGKVNPVLCETATQVSAQVIGNDAAVAFAGSQGQFELNVFIPVMARNVLESARLLANTARVFATRLVDGIVPNEEHMKQLAESSPSIVTPLNSAIGYEAAAKVAKAALAEGKTIRQTVIDMGFVDGEKLTEEELDKRLDVLAMANTDRK 470          

BLAST of fumarate hydratase class ii vs. SwissProt
Match: gi|39931506|sp|Q83CL8.2|FUMC_COXBU (RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase)

HSP 1 Score: 418.313 bits (1074), Expect = 1.480e-142
Identity = 206/441 (46.71%), Postives = 293/441 (66.44%), Query Frame = 0

Query:    5 QQRWGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIA----DLAG------DDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARG-VDAALLDQALDPLRI 434
            ++ +G+++Q+++ NF I  ET+PP ++   G LK AA   N ELG++ + +A  I+ A  E+  G+ +D FP+ I+QTGSGT TNMN NEVI+    +LAG      + VHPNDHVN+ QSSND FPTA+H+ A ++  + L P L  L   L  K+ EF++  K GRTHL DAVP+TLGQEF GY AQ+    + ++  +  + ++ LGGTAVG+GLNTHP FA+   + + E  G+   +  ++F A A  D +V +SG LKT+A SL KIA DI W+ASGPR G+GEI +P  + GSSIMPGKVNP  +E +  V  QVIGNDT ITI G QGNFELNV  P++A NLI S+ +LS +   F + C  GI  N +++    +N+L   T LN I+GYD+A+EI ++A + G++++EAAL  G + A+  D+A+DP ++
Sbjct:   16 EKYYGAQSQRSLINFAIGRETMPPELIRAFGVLKKAAVLTNVELGKVSQEKANFILKACEELIAGKLNDHFPLKIWQTGSGTQTNMNVNEVISNRAIELAGGKLGSKEPVHPNDHVNMSQSSNDTFPTAMHITAAEMITHQLIPNLTVLRDTLEKKSKEFSEIIKIGRTHLQDAVPLTLGQEFSGYVAQLNHNLEAINDVLPTLYRLALGGTAVGTGLNTHPQFAKKAADHIAELTGIPFYSASNKFAALAANDEIVLVSGVLKTLACSLMKIANDIRWLASGPRCGIGEIVIPENEPGSSIMPGKVNPTQSEAMTMVCVQVIGNDTTITIAGSQGNFELNVYKPVMAYNLIQSIYLLSDACRSFNDHCAVGIKPNQEKINDYLNNSLMLVTALNQIIGYDKASEIAKKAYKEGTTLKEAALQLGYLTASEFDKAVDPKKM 456          

BLAST of fumarate hydratase class ii vs. SwissProt
Match: gi|39931491|sp|Q82SM5.1|FUMC_NITEU (RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase)

HSP 1 Score: 416.387 bits (1069), Expect = 8.449e-142
Identity = 215/438 (49.09%), Postives = 283/438 (64.61%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLA----------GDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARG-VDAALLDQALDPLRI 434
            WG++TQ++++NF ISGE +P  ++H L  +K AAA VN +LG LD   A  I+ AA EV  G H  +FP+ ++QTGSGT TNMN NEV+A+ A          G  VHPNDHVN GQSSNDVFPTA+H+AA++   N L PAL  L   L++K+  F+D  K GRTHL DA P+TLGQEF GY +Q+  G   L++A+  + ++ LGGTAVG+GLNTHP+FAR V   +    G    T  ++FEA A  DALV   G LKT+A  L KIA D+ W+ASGPR G+GEI +P  + GSSIMPGKVNP  +E V+ +A QV+GND AI +GG  GNFELN   PLI  N + S  +L+     F   C +GITANT R+      +L   T LNP +GYD+A EI ++A     +++EAA+  G V A   D  +DP ++
Sbjct:   22 WGAQTQRSLNNFNISGERMPSALIHALALVKRAAASVNHDLGLLDENIARAIITAADEVLAGEHAGEFPLVVWQTGSGTQTNMNMNEVLANRASEILGGTRGKGRKVHPNDHVNKGQSSNDVFPTAMHIAAVEAIRNRLIPALEALRKTLSSKSAAFSDIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLAHLESALPHLLELALGGTAVGTGLNTHPEFARRVAAEIARLSGYPFITAANKFEALAAHDALVHAHGVLKTLAAILIKIANDVRWLASGPRCGIGEILIPENEPGSSIMPGKVNPTQSEAVVMLACQVMGNDVAINLGGAMGNFELNTMKPLIIHNFLQSTRLLADGAESFNTHCAAGITANTVRIKQHLQESLMLVTALNPHIGYDKAAEIAKKAHHEMLTLKEAAIRLGYVTAEQFDVWVDPQKM 459          

BLAST of fumarate hydratase class ii vs. SwissProt
Match: gi|73919610|sp|Q87DC2.1|FUMC_XYLFT (RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase)

HSP 1 Score: 416.772 bits (1070), Expect = 9.148e-142
Identity = 215/433 (49.65%), Postives = 290/433 (66.97%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLA----GDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIAR 436
            WG++TQ+AI+NFPISG ++P   +H LG +KAAAA+VNAELG L ++ A  I AAA +VA GR+D +FPVDI+QTGSGTS+NMNANEVIA LA       +HPNDHVNLGQSSNDV PTA+ ++A       L PAL  L   +  +A       K GRTHLMDA+P+T  QEFG ++AQI     RL+  + R+ ++PLGGTA+G+G+NT P F R  ++ L    G+  E+  ++FE  A +D LVELSG   T+AV+L KIA D+ WM +GP AGLGEI LP LQ GSSIMPGKVNPV+ E V+ VA+QVIG+ TA+T+ G  GNF+LNV +PLIA NL++S ++L + V + A++ + G+    DR+  + +      T LNPI+GY++A  I + A +    V E A          +  L+P+ +AR
Sbjct:   29 WGAQTQRAIENFPISGRSMPQGFIHALGFIKAAAAKVNAELGLLPKSMAKEIEAAALDVAAGRYDAEFPVDIYQTGSGTSSNMNANEVIATLAMRATKGPIHPNDHVNLGQSSNDVVPTAIRISATLAVQGRLLPALKHLRKMINKRARGLGSVVKTGRTHLMDAMPLTFAQEFGAWSAQIVSAEARLNDTLKRLHRLPLGGTAIGTGINTDPHFGRNAVKVLSALTGIHFESANNKFEGLAAQDDLVELSGQFNTLAVALMKIANDLRWMNAGPLAGLGEIELPALQPGSSIMPGKVNPVIPEAVVMVASQVIGHHTAVTVAGQSGNFQLNVTLPLIAYNLLESATLLGNVVMLLADKVIVGLKVRQDRVQEVLERNPILVTALNPIIGYEKAAVIAKRAYKEHRPVLEVACE--------ESNLNPVELAR 453          

BLAST of fumarate hydratase class ii vs. SwissProt
Match: gi|73919609|sp|Q9PD25.1|FUMC_XYLFA (RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase)

HSP 1 Score: 413.69 bits (1062), Expect = 1.530e-140
Identity = 214/433 (49.42%), Postives = 288/433 (66.51%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLA----GDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIAR 436
            WG++TQ+AI+NFPISG ++P   +H LG +KAAAA+VNAELG L +  A  I AAA +VA GR+D  FPVDI+QTGSGTS+NMNANEVIA LA     + +HPNDHVNLGQSSNDV PTA+ ++A       L PAL  L   +  +A       K GRTHLMDA+P+T  QEFG ++AQI     RL+  + R+ ++PLGGTA+G+G+NT P F R  ++ L    G+  E+  ++FE  A +D LVELSG    +AV+L KIA D+ WM +GP AGLGEI LP LQ GSSIMPGKVNPV+ E V+ VA+QVIG+ TA+T+ G  GNF+LNV +PLIA NL++S ++L + V + A++ + G+    DR+  + +      T LNPI+GY++A  I + A +    V E A          +  L+P+ +AR
Sbjct:   29 WGAQTQRAIENFPISGRSMPQGFIHALGFIKAAAAKVNAELGLLPKPMAKEIEAAALDVAAGRYDADFPVDIYQTGSGTSSNMNANEVIATLAMRATKEPIHPNDHVNLGQSSNDVVPTAIRISATLAVQGRLLPALKHLRKMINKRARGLGSVVKTGRTHLMDAMPLTFAQEFGAWSAQIVSAEARLNDTLKRLHRLPLGGTAIGTGINTDPHFGRNAVKVLSALTGIHFESANNKFEGLAAQDDLVELSGQFNALAVALMKIANDLRWMNAGPLAGLGEIELPALQPGSSIMPGKVNPVIPEAVVMVASQVIGHHTAVTVAGQSGNFQLNVTLPLIAYNLLESATLLGNVVMLLADKVIVGLKVRQDRVQEVLERNPILVTALNPIIGYEKAAVIAKRAYKEHRPVLEVACE--------ESNLNPVELAR 453          

BLAST of fumarate hydratase class ii vs. SwissProt
Match: gi|39931633|sp|Q9HQ29.1|FUMC_HALSA (RecName: Full=Fumarate hydratase class II; Short=Fumarase C; AltName: Full=Aerobic fumarase; AltName: Full=Iron-independent fumarase)

HSP 1 Score: 405.216 bits (1040), Expect = 2.112e-137
Identity = 215/421 (51.07%), Postives = 277/421 (65.80%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIA----DLAGDD-----VHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARG 419
            WG++TQ+AI+NFPISG       V  LG +K AAA+ N +LG +D  RA  IVAAA EV  G HDDQFPVD+FQTGSGTS+NMNANEVIA    +L G++     VHPNDHVN GQSSNDV PTA+H+A++D  +N + P L TL   L  KA  F    K GRTHL DA PV LGQEFGGY  Q+  G DR++A   R+ ++ LGGTAVG+GLNTHP+F       + E  GV      + FEAQA  DA+ E  GAL+T+A SL KIA D+  +ASGPR GLGEI  P  Q GSSIMPGK+NPV+AE V QV  QV+GND AI  G  +G  +LN+  P++A N + S  +L+++   F E+ V+ + AN        + ++A AT LNP +GYD+A+E+ + A + G +V E  + +G
Sbjct:   23 WGAQTQRAIENFPISGIAFGRRFVRALGVVKKAAAQANRDLGLVDDERADAIVAAADEVIAGEHDDQFPVDVFQTGSGTSSNMNANEVIANRAAELLGEEIGDRVVHPNDHVNYGQSSNDVIPTAMHVASLDALVNDVKPGLETLAAELDDKADAFDGVVKTGRTHLQDATPVRLGQEFGGYRTQVEKGIDRIEAVAPRLSELALGGTAVGTGLNTHPEFPETAAGYISEETGVTFREADNHFEAQAAHDAMNEAHGALRTVAGSLNKIANDLRLLASGPRNGLGEIEQPENQPGSSIMPGKINPVVAEAVNQVHKQVVGNDAAIAAGAAEGQIDLNLYKPVLAHNFLQSADMLANASAAFGEKFVAKLEANEAACEAQVERSMALATALNPTIGYDKASEVAKAALKEGKTVTEVVVEKG 443          

BLAST of fumarate hydratase class ii vs. nr
Match: gi|505253065|ref|WP_015440167.1| (class II fumarate hydratase [Ilumatobacter coccineus] >gi|464098037|dbj|BAN00919.1| fumarate hydratase class II [Ilumatobacter coccineus YM16-304])

HSP 1 Score: 625.165 bits (1611), Expect = 0.000e+0
Identity = 316/436 (72.48%), Postives = 361/436 (82.80%), Query Frame = 0

Query:    4 MQQRWGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLAGDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIARGNQ 439
            M + WG+ET+KAI+NFPISGE VP  V+ WLGR+K+AAA  NAELG LD   AAR+  AA E+  G H DQFP+DIFQTGSGTSTNMN NEVIA LAGDDVHPNDHVNLGQSSNDVFPTAVHLAA+D+A  +LAPAL +LE +LA KA EFAD AKAGRTHLMDAVP+TLG+EFG +A Q+ LG DR+++ + RV  VPLGGTAVG+GLNTHPDFAR V E + ER GV+V  P DRFEAQA RD+LVELSGALK +AVSLTKIA DIA +ASGPRAGLGEI LP LQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNF+LNVR+PLIARNL+ S+ IL+S+  VFAE CV+GI AN DRLAWLAD+TLASAT LNPIVGYD A  IV+ A+R G S+R+AA    VD  ++   LD  +IARGN+
Sbjct:    1 MVEYWGNETRKAIENFPISGERVPASVIRWLGRIKSAAATANAELGVLDAELAARVATAADEIGAGEHADQFPIDIFQTGSGTSTNMNVNEVIAHLAGDDVHPNDHVNLGQSSNDVFPTAVHLAALDMATTTLAPALRSLERSLAGKAAEFADLAKAGRTHLMDAVPITLGEEFGAFAHQVGLGIDRVESCLQRVAAVPLGGTAVGNGLNTHPDFARRVRELIAERTGVEVVEPGDRFEAQASRDSLVELSGALKVVAVSLTKIANDIALLASGPRAGLGEITLPALQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFQLNVRIPLIARNLLGSMEILASTSIVFAENCVAGIVANHDRLAWLADHTLASATALNPIVGYDVAGAIVQLADRDGLSLRDAARTFDVDEEVIADVLDARKIARGNR 436          

BLAST of fumarate hydratase class ii vs. nr
Match: gi|654590248|ref|WP_028057248.1| (class II fumarate hydratase [Solirubrobacter sp. URHD0082])

HSP 1 Score: 579.711 bits (1493), Expect = 0.000e+0
Identity = 290/434 (66.82%), Postives = 338/434 (77.88%), Query Frame = 0

Query:    5 QQRWGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLAGDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIARGN 438
            Q+ WG ET KA+ NFP+SGE VP  VV WLGR+K AAA  NAELG LD A A RI AA  E+A+G+HD QFP+D+FQTGSGTS+NMNANEVIA+LAG   HPNDHVN+GQSSNDVFP+AVHLAA+D A N L PAL  LE + A KA EF D  K+GRTHLMDAVPVTLGQEF GYAAQ+RLGR R+  A+ +V Q+PLGGTA G+GLNTHP+FA  V ERL    G+ +  P D FEAQ  RDALVELSGALK IAVSLTKIAGD+A M SGPRAG+GEI LP LQKGSSIMPGKVNPV+ EVVLQV AQVIGND AIT+GG+QG FELNVR+PLIARNL+ S+ +LSS+ T+ A++CV GI  N   L   A+ TLA AT LNP +GYD A EIV++A +SG ++RE A   GVD   LD+ALD  +IA G+
Sbjct:    8 QELWGGETTKAVANFPVSGERVPLPVVRWLGRIKGAAARANAELGLLDGALAERIAAAGDEIAEGKHDAQFPIDVFQTGSGTSSNMNANEVIANLAGGGAHPNDHVNMGQSSNDVFPSAVHLAALDDATNQLLPALERLEASFAAKAEEFKDLVKSGRTHLMDAVPVTLGQEFSGYAAQVRLGRRRVRNALPQVAQIPLGGTATGTGLNTHPEFAAKVRERLSADTGLTISAPEDPFEAQGNRDALVELSGALKVIAVSLTKIAGDLALMGSGPRAGIGEIFLPELQKGSSIMPGKVNPVIPEVVLQVGAQVIGNDAAITVGGLQGQFELNVRIPLIARNLLQSIHLLSSASTLLAQKCVDGIEPNLAGLEASAEGTLAVATALNPYIGYDRAAEIVKDASKSGRTLREVAREHGVDEETLDKALDLRKIAAGS 441          

BLAST of fumarate hydratase class ii vs. nr
Match: gi|1286951655|dbj|GBD45374.1| (Fumarate hydratase class II [bacterium HR41])

HSP 1 Score: 576.63 bits (1485), Expect = 0.000e+0
Identity = 290/441 (65.76%), Postives = 340/441 (77.10%), Query Frame = 0

Query:    1 MAAMQQRWGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLAGDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIARGNQEA 441
            M A  + WG+ET+KAI+NFP+SGE VP  VV WLGR+KAAAA VN ELG LD  RA RI  AA EVA G HDDQFPVD+FQTGSGTS+NMNANEVIA +AGDDVHPNDHVN+GQSSNDVFPTAVHLAA     + L PAL  LE +LA KA E+ D  KAGRTH+MDAVPVTLGQEF GYA QIRLGR R++  + RV QVPLGGTA G+GLNTHP+FAR V  ++ E+ G++   P DRFEAQ  RDALVELSGAL+ +A+SLTKIA D+  M SGPR GL E+ LP LQKGSSIMPGKVNPV+ EVVLQ+  QV+GND AI + G QGNFELNVRVPLIARNL+ S+ IL+S   + AE+CV GI AN + L   A++T A AT LNP +GYD ATEIV+EA  S  ++RE AL +GV+A +LD+ALD   +ARG   A
Sbjct:    1 MTARTELWGAETRKAIENFPVSGERVPVAVVRWLGRIKAAAARVNGELGLLDTDRAERIARAADEVAAGMHDDQFPVDVFQTGSGTSSNMNANEVIARIAGDDVHPNDHVNMGQSSNDVFPTAVHLAAAAEVRDDLMPALERLERSLAAKAEEWRDVVKAGRTHMMDAVPVTLGQEFAGYATQIRLGRQRIEDTLPRVLQVPLGGTATGTGLNTHPEFARRVHRKIQEQTGLEPREPEDRFEAQGNRDALVELSGALRVLAISLTKIANDLVLMGSGPRCGLAELQLPELQKGSSIMPGKVNPVIPEVVLQIGNQVVGNDAAIAMAGSQGNFELNVRVPLIARNLLHSIKILASGARLLAEKCVDGIVANVEALRRYAESTPAIATALNPYIGYDRATEIVKEAVASRRTIREVALEKGVEADVLDRALDLAAMARGESTA 441          

BLAST of fumarate hydratase class ii vs. nr
Match: gi|1225103096|ref|WP_093116322.1| (class II fumarate hydratase [Thermoleophilum album] >gi|1093219024|emb|SEH11371.1| fumarase, class II [Thermoleophilum album])

HSP 1 Score: 567.385 bits (1461), Expect = 0.000e+0
Identity = 284/420 (67.62%), Postives = 331/420 (78.81%), Query Frame = 0

Query:    1 MAAMQQRWGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLAGDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGV 420
            M A  + WG+ET+KAI+NFP+SGE VP  VV WLGR+KAAAA VN ELG LD  RA RI  AA EVA G HDDQFPVD+FQTGSGTS+NMNANEVIA LAGDDVHPNDHVN+GQSSNDVFPTAVHLAA     + L PAL  LE +L+ KA E+ D  KAGRTH+MDAVPVTLGQEF GYAAQIRLGR+R+ AA+ RV QVPLGGTA G+GLNTHP+FA  V  ++ E+ G++   PLDRFEAQA RDALVELSGAL+ +AVSLTKIA D+  M SGPR GL E+ +P LQKGSSIMPGKVNPV+ EVVLQ+A QV+GND AI + G QGNFELNVRVPLIARNL+ S+ IL+S   + AE+CV G+ AN + L   A++T A AT LNP +GYD ATEIV+EA  S  ++RE AL +GV
Sbjct:    1 MTARTELWGAETRKAIENFPVSGERVPTAVVRWLGRIKAAAARVNGELGLLDPDRAERIARAADEVAAGMHDDQFPVDVFQTGSGTSSNMNANEVIARLAGDDVHPNDHVNMGQSSNDVFPTAVHLAAAAEVRDDLLPALERLERSLSAKAEEWRDVVKAGRTHMMDAVPVTLGQEFAGYAAQIRLGRERVLAALPRVLQVPLGGTATGTGLNTHPEFASRVHRKIHEQTGLEPREPLDRFEAQANRDALVELSGALRVLAVSLTKIANDLVLMGSGPRCGLAELQIPELQKGSSIMPGKVNPVIPEVVLQIANQVVGNDAAIAMAGSQGNFELNVRVPLIARNLLHSIKILASGSRLLAEKCVDGLVANVEVLRRYAESTPAIATALNPYIGYDRATEIVKEAVASQRTIREVALEKGV 420          

BLAST of fumarate hydratase class ii vs. nr
Match: gi|654597430|ref|WP_028064393.1| (class II fumarate hydratase [Solirubrobacter soli])

HSP 1 Score: 563.533 bits (1451), Expect = 0.000e+0
Identity = 281/431 (65.20%), Postives = 333/431 (77.26%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLAGDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIARGN 438
            WG ET KA+ NFP+SGE VP  VV WLGR+K  AA VNAELG LD   A +I AA  E+A+G+HD QFP+D+FQTGSGTS+NMN+NEVIA L+G   HPNDHVN+GQSSNDVFP+AVHLAA+D A N+L PALA LE + + KA  + D  K+GRTHLMDAVPVTLGQEF GYAAQIRLG+ R+  A+ +V Q+PLGGTA G+GLNTH DFA  V E+L    G+ +  P D FEAQ  RDALVELSGALK +AVSLTKIAGD+A M SGPR G+GEI LP LQKGSSIMPGKVNPV+ EVVLQV+AQVIGNDTAIT+GG+QG FELNVR+PLIARNL+ S+++LSS+  +FA +CV GI  N   L   A+ TLA AT LNP +GYD A  IV+EA  SG ++RE A   GVD   LD+ALD  +IA G+
Sbjct:   11 WGGETTKAVANFPVSGERVPLPVVRWLGRIKGTAARVNAELGLLDAELAEKIAAAGDEIAEGKHDAQFPIDVFQTGSGTSSNMNSNEVIATLSG--AHPNDHVNMGQSSNDVFPSAVHLAALDEATNTLLPALAQLEASFSAKAEAYKDLVKSGRTHLMDAVPVTLGQEFAGYAAQIRLGQKRIANALPQVAQIPLGGTATGTGLNTHRDFAAKVREKLSADTGLTISAPEDPFEAQGNRDALVELSGALKVVAVSLTKIAGDLALMGSGPRVGIGEIFLPELQKGSSIMPGKVNPVIPEVVLQVSAQVIGNDTAITVGGLQGQFELNVRIPLIARNLLQSIALLSSTAKIFASKCVDGIEPNLAGLENSAEGTLAVATALNPFIGYDRAAAIVKEASASGGTLREVARKHGVDEETLDKALDLRKIAAGS 439          

BLAST of fumarate hydratase class ii vs. nr
Match: gi|502702010|ref|WP_012937305.1| (class II fumarate hydratase [Conexibacter woesei] >gi|283951510|gb|ADB54254.1| fumarate lyase [Conexibacter woesei DSM 14684])

HSP 1 Score: 557.755 bits (1436), Expect = 0.000e+0
Identity = 287/434 (66.13%), Postives = 339/434 (78.11%), Query Frame = 0

Query:    5 QQRWGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLAGDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIARGN 438
            +Q WG ET KA+ NFP+SGE VP  VV WLGR+K AAA VN ELG LD   A RI AA  E+A G+HD QFPVD+FQTGSGTS+NMNANEVIA L+G   H NDHVN+GQSSNDVFP+AVHLAA+D A N L PAL  LE A A KA  F D  K+GRTHLMDAVPVTLGQEF GYAAQIRLG  R+  A+ +V Q+PLGGTA G+GLNTHP+FA  V  +L E  G+++  P D FEAQ  RDALVELSGALK IAVSLTKIA D+A M SGPRAG+GE+ LP LQKGSSIMPGKVNPV+ EVVLQV+AQVIGNDTAIT+GG+QG FELNVR+PLIARNL+ S+S+L+++  +FAE+CV GITAN       A++TLA AT LNP +GYD+A+EIV+EA  SG ++RE AL +GV     ++A+D  RIARG+
Sbjct:    7 EQLWGGETTKAVANFPVSGERVPVPVVRWLGRIKGAAARVNGELGLLDGEIAERIAAAGDEIAAGKHDGQFPVDVFQTGSGTSSNMNANEVIATLSG--AHQNDHVNMGQSSNDVFPSAVHLAALDEATNDLLPALERLEAAFAAKAAAFEDIVKSGRTHLMDAVPVTLGQEFSGYAAQIRLGAKRVRNALPQVAQIPLGGTATGTGLNTHPEFAARVRGKLAESSGLEILPPEDPFEAQGNRDALVELSGALKVIAVSLTKIANDLALMGSGPRAGIGELFLPELQKGSSIMPGKVNPVIPEVVLQVSAQVIGNDTAITVGGLQGQFELNVRIPLIARNLLQSISLLANTSRLFAEKCVEGITANEAGCERSAESTLAVATALNPFIGYDKASEIVKEAASSGRTLREVALEKGVSEETYNEAIDLRRIARGS 438          

BLAST of fumarate hydratase class ii vs. nr
Match: gi|1125520102|gb|OLE38010.1| (aspartate ammonia-lyase [Actinobacteria bacterium 13_1_20CM_3_68_9])

HSP 1 Score: 545.043 bits (1403), Expect = 0.000e+0
Identity = 281/432 (65.05%), Postives = 331/432 (76.62%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLAGDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVKVETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIARGNQ 439
            WG ET KA+ NFP+SG+ VP  VV WLGR+KAAAA VNAEL  LD   A RI  A   VA G HDDQFP+D+FQTGSGTS+N NANEVIA LAGD+VHPNDHVN+GQSSNDVFP+AVHLAA+    + L PA+  L +AL  KA EFAD  KAGRTHLMDAVPVTLGQEFGGYAAQIRLG +R+ A +  V Q+PLGGTA G+GLNTH +FA+ V  +L E  G+++  PLDRFEAQA RDALVELSG LK +AVSLTKIA DIA M SGPRAGLGE+ LP LQKGSSIMPGKVNPV+ EV +QV AQVIGND AIT+ G QG FELNVRVPLIARNL+DS+ +L ++  +   +CV GI A+ +     A+ TLA+A  LNP +GYD ATEIV+EA  SG  +RE A   GV+  +L+QALD  R+AR ++
Sbjct:   10 WGVETSKAVSNFPVSGQPVPVPVVRWLGRIKAAAARVNAELRLLDPQVAERIATAGDAVAAGEHDDQFPIDVFQTGSGTSSNTNANEVIATLAGDEVHPNDHVNMGQSSNDVFPSAVHLAALAEVAHDLLPAMDGLASALERKAEEFADVVKAGRTHLMDAVPVTLGQEFGGYAAQIRLGMERVRATLGHVGQIPLGGTATGTGLNTHSEFAQRVRAKLTEETGIEISEPLDRFEAQANRDALVELSGMLKVVAVSLTKIANDIALMGSGPRAGLGELRLPELQKGSSIMPGKVNPVICEVAIQVGAQVIGNDAAITVAGSQGQFELNVRVPLIARNLLDSIKLLGAACRLLDTKCVQGIEADEEMAERHAEATLATAAALNPHIGYDRATEIVKEAHASGRPLREVAREMGVEEEILEQALDHRRMARPHE 441          

BLAST of fumarate hydratase class ii vs. nr
Match: gi|652515833|ref|WP_026910037.1| (class II fumarate hydratase [Patulibacter minatonensis])

HSP 1 Score: 544.658 bits (1402), Expect = 0.000e+0
Identity = 284/433 (65.59%), Postives = 335/433 (77.37%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLAGDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVK-VETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIARGNQ 439
            +GSETQKA+DNFPISGE VP  V+ W  R+K AAA VNAELG+LD+  A +I  AAG++  G HDDQFP+D+FQTGSGTSTNMN NEVI+ L G + H NDHVN+GQSSND FP+AVHLAA+D A N+L PAL  LE +L  KA EF D  KAGRTHLMDAVPVTLGQEF GYAAQ+R G  R++AA+  V Q+PLGGTA G+GLNT  +FA  + E+L    G++ +  P D FEAQ  RDALVELSGALK +AVSLTKIA DIA M SGPR G+GE+ LP LQKGSSIMPGKVNPVL EVV+QV+AQVIGNDTAITIGGMQG FELNVRVPLIARNL+ S+ +LS++ T F+ +CV GITANT      A  TLA+AT LN  +GYD AT IV++A  SG  +R+ ALA GVDA L D+ +D  +IARGNQ
Sbjct:    6 YGSETQKAVDNFPISGERVPIQVIRWQARIKGAAALVNAELGKLDQGIADQISEAAGKIVAGDHDDQFPIDVFQTGSGTSTNMNVNEVISALTGGEAHRNDHVNMGQSSNDTFPSAVHLAALDEATNTLIPALKQLEASLQAKADEFVDVVKAGRTHLMDAVPVTLGQEFSGYAAQVRQGVKRVEAALPHVGQIPLGGTATGTGLNTPAEFAPAIREKLIADSGLELIAAPADPFEAQGNRDALVELSGALKVVAVSLTKIAQDIALMGSGPRVGIGELFLPELQKGSSIMPGKVNPVLPEVVIQVSAQVIGNDTAITIGGMQGQFELNVRVPLIARNLLQSIHLLSTASTSFSTKCVDGITANTKGTDASAAGTLATATALNEAIGYDLATTIVKKATDSGEPLRDVALAEGVDADLFDKTIDLRKIARGNQ 438          

BLAST of fumarate hydratase class ii vs. nr
Match: gi|551310290|ref|WP_022930199.1| (class II fumarate hydratase [Patulibacter americanus])

HSP 1 Score: 538.495 bits (1386), Expect = 0.000e+0
Identity = 285/433 (65.82%), Postives = 337/433 (77.83%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLAGDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVK-VETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIARGNQ 439
            +GSETQKA++NFPISGE VP  V+ W  R+K AAA+VNA+LG+LD+  A  I  AA  +  G+HDDQFP+D+FQTGSGTSTNMN NEVI+ L G+  H NDHVN+GQSSND FP+AVHLAA+D A N L PALA LE +L  KA EFAD  KAGRTHLMDAVPVTLGQEF GYAAQ+RLG+ R++ A+ +V Q+PLGGTA G+GLNT  +FA  V ERL    G+  +  P D FEAQA RDALVELSGALK++AVSLTKIA DIA M SGPRAG+GE+ LP LQKGSSIMPGKVNPVL EVVLQV+AQVIGNDTAITIGGMQG FELNVRVPLIARNL+ S+ +LS++   FAE+ + GI AN       A  TLA+AT LN  +GYD+ATEIV++A  SG  +R+ ALA GVDA L D+ +D  +IA GNQ
Sbjct:    6 YGSETQKAVENFPISGERVPIQVIRWQARIKGAAAQVNADLGKLDQGIADAISEAAAAIVAGQHDDQFPIDVFQTGSGTSTNMNVNEVISALTGEKAHRNDHVNMGQSSNDTFPSAVHLAALDEATNRLLPALAQLEASLQAKADEFADVVKAGRTHLMDAVPVTLGQEFAGYAAQLRLGQKRVENALPQVGQIPLGGTATGTGLNTPKEFAPQVRERLIADSGLDLISGPEDAFEAQANRDALVELSGALKSVAVSLTKIAQDIALMGSGPRAGIGELFLPELQKGSSIMPGKVNPVLPEVVLQVSAQVIGNDTAITIGGMQGQFELNVRVPLIARNLLQSIHLLSTASKAFAEKAIDGIRANQAGTDASAGATLATATALNEAIGYDKATEIVKKATESGEPLRDVALAEGVDADLYDRTIDLRKIAAGNQ 438          

BLAST of fumarate hydratase class ii vs. nr
Match: gi|654609448|ref|WP_028074202.1| (class II fumarate hydratase [Solirubrobacterales bacterium URHD0059])

HSP 1 Score: 535.413 bits (1378), Expect = 0.000e+0
Identity = 285/433 (65.82%), Postives = 331/433 (76.44%), Query Frame = 0

Query:    8 WGSETQKAIDNFPISGETVPPVVVHWLGRLKAAAAEVNAELGQLDRARAARIVAAAGEVADGRHDDQFPVDIFQTGSGTSTNMNANEVIADLAGDDVHPNDHVNLGQSSNDVFPTAVHLAAIDLALNSLAPALATLETALATKATEFADHAKAGRTHLMDAVPVTLGQEFGGYAAQIRLGRDRLDAAVVRVRQVPLGGTAVGSGLNTHPDFARLVIERLGERGGVK-VETPLDRFEAQAGRDALVELSGALKTIAVSLTKIAGDIAWMASGPRAGLGEIALPTLQKGSSIMPGKVNPVLAEVVLQVAAQVIGNDTAITIGGMQGNFELNVRVPLIARNLIDSLSILSSSVTVFAERCVSGITANTDRLAWLADNTLASATVLNPIVGYDEATEIVREAERSGSSVREAALARGVDAALLDQALDPLRIARGNQ 439
            +G ET+KA+DNFPISGETVP  V+ WLGR+K  AA VNAELG +D+  AA I AA   +A G HD+QFP+D+FQTGSGTSTNMN NEVI+ L     HPNDHVN GQSSNDVFP+AVHLAA+D A N L PAL  LE +L  KA  F D  K+GRTHLMDAVPVTLG EF GYAAQIRLG++R+ +A+V V Q+PLGGTA G+GLNTHP FA  V +RL +   +K +  P D FEAQA RDALVELSGALK +AVSLTKIA DIA M SGPRAG+GEI LP LQKGSSIMPGKVNPVL EVVLQV+AQVIGNDTAITIGGMQG FELNVR+PLIARNL+ S+ +LS++   FAE+CV GI  N       A+ TLA+AT LN  +GYD A  IV+EA  SG  +RE AL  GVDA L D+ ++  +IA GNQ
Sbjct:    9 YGGETKKAVDNFPISGETVPISVIRWLGRIKGTAALVNAELGNIDKGLAAEIAAAGEAIAAGDHDNQFPIDVFQTGSGTSTNMNTNEVISALTDGKAHPNDHVNFGQSSNDVFPSAVHLAALDEATNKLLPALKQLERSLDRKARRFKDVVKSGRTHLMDAVPVTLGAEFAGYAAQIRLGQERIQSALVHVGQIPLGGTATGTGLNTHPQFAAAVRKRLAKDSKLKLIAPPADPFEAQANRDALVELSGALKVVAVSLTKIAQDIALMGSGPRAGIGEIFLPELQKGSSIMPGKVNPVLPEVVLQVSAQVIGNDTAITIGGMQGQFELNVRIPLIARNLLQSIHLLSTTSVAFAEKCVDGIEVNKAGTKASAEATLAAATALNGAIGYDAAAAIVKEASASGRPLREVALEAGVDAKLYDETINLRKIAAGNQ 441