BLAST of putative sulfatase vs. L. salmonis genes
Match: EMLSAG00000012454 (supercontig:LSalAtl2s:LSalAtl2s915:37892:77268:-1 gene:EMLSAG00000012454 transcript:EMLSAT00000012454 description:"maker-LSalAtl2s915-augustus-gene-0.22")

HSP 1 Score: 200.675 bits (509), Expect = 2.094e-58
Identity = 133/396 (33.59%), Postives = 196/396 (49.49%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDA-GYATLAVGKWH--CGDQPDFLPT-NHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQV-PAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDI-RGLLGLDGPTPSADRSFFYIGDANIE----AVRVGRWKLHV 388
            PNII+   DDLGYGDL  YG     TP +D +  +    +++Y  SPV SPSRAS+ TG YP+R G       +  V FP    GL+P + T+   L D  GY +  +GKWH   G   ++LPT +  FD ++G+PYS+DM         P  E    +  ++  +    + P PL E   + E   + + LT +Y  R + FI+++++  +FL  A    H P +   +F   S  G++G A++ +D A   I++ L +  L  +T+VIFTS NG         G   P R  KGTT+EGG RVP  + W G + P  + V  + TALD+ PTL +    +       DG D  R +L         +   ++ G AN      AVR   +K H 
Sbjct:  166 PNIIMFVADDLGYGDLSYYGHPTSSTPHIDNIISKSMWFSNYYTTSPVSSPSRASLFTGEYPMRSGI------YPGVFFPNSTGGLDPSKKTIMKELRDRFGYKSALIGKWHLGVGKNAEYLPTVSKNFDSYYGIPYSHDMC--------PCTECFPNKECSSSCN--SNFVPCPLFEDNGIKEQPTNLSKLTQKYTNRAIQFIKSNRNNSYFLTYAFHQTHDPQFAGLKFVNTSIRGSFGDALSEMDDAIGQIMQFLKKSDLLTETLVIFTSANGPNISRQGQGGCAGPFRCGKGTTYEGGVRVPLFMHWSGFIQPYKSHV--LITALDIYPTLMSMVSVQ--YKNSTDGFDFTRHILKQKLDYNPRELLLYFPGHANKTIGPYAVRYKNYKAHF 541          

BLAST of putative sulfatase vs. L. salmonis genes
Match: EMLSAG00000012909 (supercontig:LSalAtl2s:LSalAtl2s981:76751:80020:-1 gene:EMLSAG00000012909 transcript:EMLSAT00000012909 description:"maker-LSalAtl2s981-augustus-gene-0.25")

HSP 1 Score: 114.775 bits (286), Expect = 2.507e-27
Identity = 107/394 (27.16%), Postives = 171/394 (43.40%), Query Frame = 0

Query:   13 LGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGD-QPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENH-----QDEPFFLYLAHMYVH---------LPIYVEERFH----EGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFD-GSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKL 386
            +G+ D+G +GS    TP +DALA  G  L ++Y+ +P+C+PSR++++TG +P+  G        +  +  G   GL  +   +   L++ GY + A+GKWH G  +  + PT  GF    G  Y    G              ++R    G DF          +  +L        S T  +     + I  H     +++  FLYLAH  VH          P    +RF     +G R   + A + ++D +   +I  L +  +  D+I+IFT+DNG  A GF+    SN PLR  K T WEGG R   +  W    P   +  +   ++DL+         +  A  ++DG D+   L    P+P          +    A+R+G WK 
Sbjct:  243 VGWNDVGFHGSNQIPTPNIDALAYSGKILNNYYV-NPICTPSRSALMTGLHPIHTGL------QLHTIPAGAPYGLPLNLKILPEYLNELGYESHAIGKWHLGSFRKVYTPTYRGFKSHHG--YWQGAGDYF--------GHTHSRKSEWGYDF---------YKNMDLDYKSFGNYS-TKIFTSSAEEIINTHGSAKNENKSLFLYLAHQAVHSATGGDPLQAPSEDIKRFQYIEDQGRR--IFAADLYNLDKSVGSVISALRKNDMLKDSIIIFTTDNGGPAAGFNENRASNWPLRGVKDTLWEGGVRGAGIF-WS---PEIALNKKKEMSMDLM-------SGKKNALPMMDGFDLWPTLTQGTPSPRNVLLHNIDEERFASAIRIGPWKF 596          

BLAST of putative sulfatase vs. L. salmonis genes
Match: EMLSAG00000000731 (supercontig:LSalAtl2s:LSalAtl2s1117:130402:134840:1 gene:EMLSAG00000000731 transcript:EMLSAT00000000731 description:"maker-LSalAtl2s1117-augustus-gene-1.21")

HSP 1 Score: 103.99 bits (258), Expect = 1.084e-24
Identity = 61/178 (34.27%), Postives = 92/178 (51.69%), Query Frame = 0

Query:  173 PLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDD 350
            PL E   + E   D  +LT RY  +  +F++++ D PFFL  A    H P +   +F   S  G  G A+  +D A   I+++L  LGL   T+++FTSDNG      +  G     R  KGTT+EGG RVP  + W G V   T    + T+LD+ PTL +    +  ++ + DG++
Sbjct:   91 PLFENDVIKEQPADLTTLTQRYTNKAKEFLKSNIDNPFFLTYAFHQPHHPQFAGSKFTNSSFRGGIGDALNEMDDAVGQILDSLKELGLRSKTLILFTSDNGPSLQRQERGGCAGLFRCGKGTTYEGGVRVPFFMHWGGFVHPWT-SHTLITSLDIYPTLMSMISYQ--SNELSDGNN 265          

HSP 2 Score: 76.2554 bits (186), Expect = 2.343e-15
Identity = 34/64 (53.12%), Postives = 44/64 (68.75%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLR 66
            PN+IL   DDLGYGDL  YG    +TP +D +  +    T++Y ASP+CSPSRAS++TG YP R
Sbjct:   24 PNVILFIADDLGYGDLSGYGHPTSNTPKIDKIISKSMWFTNYYAASPICSPSRASIITGEYPSR 87          

BLAST of putative sulfatase vs. L. salmonis genes
Match: EMLSAG00000008262 (supercontig:LSalAtl2s:LSalAtl2s49:871918:905802:-1 gene:EMLSAG00000008262 transcript:EMLSAT00000008262 description:"maker-LSalAtl2s49-snap-gene-9.21")

HSP 1 Score: 89.3521 bits (220), Expect = 1.874e-20
Identity = 50/140 (35.71%), Postives = 70/140 (50.00%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFL------PTNHGFDEWFGLPYS 136
            PNII +  DDLG   L  YG+     P ++ +  +GAK       + +C+PSR++ LTG YP R                   VGL  +E+T A++    GY+T AVGKWH G    F       P  HGFD ++GLP++
Sbjct:   27 PNIIXLLADDLGRESLPSYGNNEEKVPNVERIFTEGAKFNHLLSPAALCTPSRSAFLTGRYPYRF------------------VGLPRNESTWANLAQSLGYSTKAVGKWHMGMDEIFWGDQEHGPLTHGFDSFYGLPFT 148          

BLAST of putative sulfatase vs. SwissProt
Match: gi|122138594|sp|Q32KH5.1|GALNS_CANFA (RecName: Full=N-acetylgalactosamine-6-sulfatase; AltName: Full=Chondroitinsulfatase; Short=Chondroitinase; AltName: Full=Galactose-6-sulfate sulfatase; AltName: Full=N-acetylgalactosamine-6-sulfate sulfatase; Short=GalNAc6S sulfatase; Flags: Precursor)

HSP 1 Score: 247.284 bits (630), Expect = 6.891e-75
Identity = 150/400 (37.50%), Postives = 208/400 (52.00%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPD-ETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELV---------ECQPDQASLTGRYVERCVDFIENHQ--DEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGA-LAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIR-GLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHV 388
            PNI+L+  DD+G+GDLG YG    +TP LD +A +G     FY A+P+CSPSRA++LTG  P+R GF   +        P + VG  PD E  +  +L +AGY +  VGKWH G +P F P  HGFDEWFG P  +                 + R R          P +P+    E+V           +  +A+LT  Y++  +DFI+  Q    PFFLY A    H P+Y    F   S+ G YG AV  ID +   I+  L  L ++++T V FTSDNGA L    +  GSN P    K TT+EGG R P +  WPG++PAG +  ++ + +DL  T  +  G  PP+DR+IDG D+   +LG        DR  FY     + AV +G++K H 
Sbjct:   30 PNILLLLMDDMGWGDLGIYGEPSRETPNLDRMAAEGMLFPSFYSANPLCSPSRAALLTGRLPIRNGFYTTNRHARNAYTPQEIVGGIPDQEHVLPELLKEAGYVSKIVGKWHLGHRPQFHPLKHGFDEWFGSPNCH-------------FGPYDNRAR----------PNIPVYRDWEMVGRYYEEFPINLKTGEANLTQVYLQEALDFIKRQQAAQRPFFLYWAIDATHAPVYASRPFLGTSQRGRYGDAVREIDNSVGKILSLLQDLRISENTFVFFTSDNGAALISAPNQGGSNGPFLCGKQTTFEGGMREPAIAWWPGRIPAGRVSHQLGSIMDLFTTSLSLAGLAPPSDRVIDGLDLLPAMLGGQ----LTDRPIFYYRGDTLMAVTLGQYKAHF 402          

BLAST of putative sulfatase vs. SwissProt
Match: gi|114221|sp|P15289.3|ARSA_HUMAN (RecName: Full=Arylsulfatase A; Short=ASA; AltName: Full=Cerebroside-sulfatase; Contains: RecName: Full=Arylsulfatase A component B; Contains: RecName: Full=Arylsulfatase A component C; Flags: Precursor)

HSP 1 Score: 244.202 bits (622), Expect = 8.224e-74
Identity = 164/437 (37.53%), Postives = 223/437 (51.03%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPD--FLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIEN--HQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDAN----IEAVRVGRWKLHVRKGGK--------------------EICELYDLVDDVGESIDV 411
            PNI+LI  DDLGYGDLGCYG     TP LD LA  G + TDFY+   +C+PSRA++LTG  P+R+G       +  VL P    GL  +E T+A +L+  GY T   GKWH G  P+  FLP + GF  + G+PYS+D G    +   P A   +      G D  L   P+PL+    +    P    L  RY+    D + +   QD PFFLY A  + H P +  + F E S  G +G ++  +D A   ++  +  LGL ++T+VIFT+DNG         G +  LR  KGTT+EGG R P L  WPG +  G +  E+ ++LDLLPTLAA  GA  P +  +DG D+  LL   G +P     FFY    +    + AVR G++K H    G                     E   LYDL  D GE+ ++
Sbjct:   21 PNIVLIFADDLGYGDLGCYGHPSSTTPNLDQLAAGGLRFTDFYVPVSLCTPSRAALLTGRLPVRMGM------YPGVLVPSSRGGLPLEEVTVAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPATPCDG-----GCDQGLV--PIPLLANLSVEAQPPWLPGLEARYMAFAHDLMADAQRQDRPFFLYYASHHTHYPQFSGQSFAERSGRGPFGDSLMELDAAVGTLMTAIGDLGLLEETLVIFTADNGPETMRMSRGGCSGLLRCGKGTTYEGGVREPALAFWPGHIAPG-VTHELASSLDLLPTLAALAGAPLP-NVTLDGFDLSPLLLGTGKSPRQSL-FFYPSYPDEVRGVFAVRTGKYKAHFFTQGSAHSDTTADPACHASSSLTAHEPPLLYDLSKDPGENYNL 441          

BLAST of putative sulfatase vs. SwissProt
Match: gi|123779981|sp|Q32KJ6.1|GALNS_RAT (RecName: Full=N-acetylgalactosamine-6-sulfatase; AltName: Full=Chondroitinsulfatase; Short=Chondroitinase; AltName: Full=Galactose-6-sulfate sulfatase; AltName: Full=N-acetylgalactosamine-6-sulfate sulfatase; Short=GalNAc6S sulfatase; Flags: Precursor)

HSP 1 Score: 243.817 bits (621), Expect = 1.608e-73
Identity = 148/396 (37.37%), Postives = 209/396 (52.78%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPD-ETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLP------YSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFI--ENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGA-LAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHV 388
            PNI+L+  DD+G+GDLG YG    +TP LD +A +G     FY A+P+CSPSRA++LTG  P+R GF   +        P + +G  P+ E  +  +L  AGY    VGKWH G +P F P  HGFDEWFG P      Y N +   + V       E+  R       F  ++P          +  +  +A+LT  Y++  +DFI  ++ +  PFFLY A    H P+Y  ++F   S  G YG AV  ID +   I+  L  LG++ +T V FTSDNGA L       GSN P    K TT+EGG R P +  WPG + AG +  ++ + +DL  T  +  G +PP+DR+IDG D+   + L G     DR  FY     + AV +G++K H+
Sbjct:   32 PNIVLLLMDDMGWGDLGVYGEPSRETPNLDRMAAEGMLFPSFYSANPLCSPSRAALLTGRLPIRNGFYTTNAHARNAYTPQEIMGGIPNSEHLLPELLKKAGYTNKIVGKWHLGHRPQFHPLKHGFDEWFGSPNCHFGPYDNKVKPNIPVY---RDWEMVGR-------FYEEFP----------INLKTGEANLTQLYLQEALDFIRTQHARQSPFFLYWAIDATHAPVYASKQFLGTSLRGRYGDAVREIDDSVGKILSLLQNLGISKNTFVFFTSDNGAALISAPKEGGSNGPFLCGKQTTFEGGMREPAIAWWPGHIAAGQVSHQLGSIMDLFTTSLSLAGLKPPSDRVIDGLDLLPTM-LQGHI--IDRPIFYYRGNTLMAVTLGQYKAHL 404          

BLAST of putative sulfatase vs. SwissProt
Match: gi|75054309|sp|Q8WNQ7.1|GALNS_PIG (RecName: Full=N-acetylgalactosamine-6-sulfatase; AltName: Full=Chondroitinsulfatase; Short=Chondroitinase; AltName: Full=Galactose-6-sulfate sulfatase; AltName: Full=N-acetylgalactosamine-6-sulfate sulfatase; Short=GalNAc6S sulfatase; Flags: Precursor)

HSP 1 Score: 243.047 bits (619), Expect = 3.970e-73
Identity = 155/427 (36.30%), Postives = 212/427 (49.65%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPD-ETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELV---------ECQPDQASLTGRYVERCVDFIENHQ--DEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGA-LAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHVRKGGKEICELYDLVDDV-GESIDVADDH 415
            PNI+L+  DD+G+GDLG YG    +TP LD +A +G     FY A+P+CSPSRA++LTG  P+R GF   +        P + VG  PD E  +  +L  AGYA+  VGKWH G +P F P  HGFDEWFG P  +                 + R R          P +P+    E+V           +  +++LT  Y++  +DFI+  Q    PFFLY A    H P+Y    F   S+ G YG AV  ID +   I+  L  L +A +T V FTSDNGA L       GSN P    K TT+EGG R P +  WPG +PAG +  ++ + +DL  T  +  G EPP+DR IDG D+   + L G     +R  FY     + A  +G++K H         E    VD   G+++     H
Sbjct:   30 PNILLLLMDDMGWGDLGVYGEPSRETPNLDRMAAEGMLFPSFYAANPLCSPSRAALLTGRLPIRTGFYTTNGHARNAYTPQEIVGGIPDPEHLLPELLKGAGYASKIVGKWHLGHRPQFHPLKHGFDEWFGSPNCH-------------FGPYDNRAR----------PNIPVYRDWEMVGRFYEEFPINLKTGESNLTQIYLQEALDFIKRQQATHHPFFLYWAIDATHAPVYASRAFLGTSQRGRYGDAVREIDDSVGRIVGLLRDLKIAGNTFVFFTSDNGAALVSAPKQGGSNGPFLCGKQTTFEGGMREPAIAWWPGHIPAGQVSHQLGSVMDLFTTSLSLAGLEPPSDRAIDGLDLLPAM-LQGRL--TERPIFYYRGNTLMAATLGQYKAHFWTWTNSWEEFRQGVDFCPGQNVSGVTTH 430          

BLAST of putative sulfatase vs. SwissProt
Match: gi|462148|sp|P34059.1|GALNS_HUMAN (RecName: Full=N-acetylgalactosamine-6-sulfatase; AltName: Full=Chondroitinsulfatase; Short=Chondroitinase; AltName: Full=Galactose-6-sulfate sulfatase; Short=GalN6S; AltName: Full=N-acetylgalactosamine-6-sulfate sulfatase; Short=GalNAc6S sulfatase; Flags: Precursor)

HSP 1 Score: 241.891 bits (616), Expect = 7.978e-73
Identity = 153/398 (38.44%), Postives = 205/398 (51.51%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPD-ETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELV---------ECQPDQASLTGRYVERCVDFIENH-QDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGA-LAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHV 388
            PNI+L+  DD+G+GDLG YG    +TP LD +A +G    +FY A+P+CSPSRA++LTG  P+R GF   +        P + VG  PD E  +  +L  AGY +  VGKWH G +P F P  HGFDEWFG P  +               +  AR            P +P+    E+V           +  +A+LT  Y++  +DFI+   +  PFFLY A    H P+Y  + F   S+ G YG AV  ID +   I+E L  L +AD+T V FTSDNGA L    +  GSN P    K TT+EGG R P L  WPG V AG +  ++ + +DL  T  A  G  PP+DR IDG ++   L L G     DR  FY     + A  +G+ K H 
Sbjct:   31 PNILLLLMDDMGWGDLGVYGEPSRETPNLDRMAAEGLLFPNFYSANPLCSPSRAALLTGRLPIRNGFYTTNAHARNAYTPQEIVGGIPDSEQLLPELLKKAGYVSKIVGKWHLGHRPQFHPLKHGFDEWFGSPNCHF-----------GPYDNKAR------------PNIPVYRDWEMVGRYYEEFPINLKTGEANLTQIYLQEALDFIKRQARHHPFFLYWAVDATHAPVYASKPFLGTSQRGRYGDAVREIDDSIGKILELLQDLHVADNTFVFFTSDNGAALISAPEQGGSNGPFLCGKQTTFEGGMREPALAWWPGHVTAGQVSHQLGSIMDLFTTSLALAGLTPPSDRAIDGLNLLPTL-LQGRL--MDRPIFYYRGDTLMAATLGQHKAHF 402          

BLAST of putative sulfatase vs. SwissProt
Match: gi|122132221|sp|Q08DD1.1|ARSA_BOVIN (RecName: Full=Arylsulfatase A; Short=ASA; AltName: Full=Cerebroside-sulfatase; Flags: Precursor)

HSP 1 Score: 239.58 bits (610), Expect = 4.948e-72
Identity = 162/453 (35.76%), Postives = 225/453 (49.67%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPD--FLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIEN--HQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDAN----IEAVRVGRWKLHVRKGGK--------------------EICELYDLVDDVGESIDVADDHPDTVADLLARIE 427
            PNI+LI  DDLGYGDLG YG     TP LD LA  G + TDFY+   +C+PSRA++LTG  P+R+G       +  VL P    GL  DE T+A +L+  GY T   GKWH G  P+  FLP +HGF  + G+PYS+D G    +   P A           +       P+PL+    +    P    L  RYV    D + +  HQ  PFFLY A  + H P +  + F   S  G +G ++  +D A   ++  +  LGL  +T+V FT+DNG         G +  LR  KGTT+EGG R P L  WPG +  G +  E+ ++LDLLPTLAA  GA+ P +  +DG D+  LL   G +P     FFY    +    + AVR G++K H    G                     E   L+DL +D GE+ ++ D   +   + L  ++
Sbjct:   21 PNILLIFADDLGYGDLGSYGHPSSTTPNLDQLAAGGLRFTDFYVPVSLCTPSRAALLTGRLPVRMGL------YPGVLEPSSRGGLPLDEVTLAEVLAAQGYLTGIAGKWHLGVGPEGAFLPPHHGFHRFLGIPYSHDQGPCQNLTCFPPATPCEGICDQGLV-------PIPLLANLSVEAQPPWLPGLEARYVAFARDLMTDAQHQGRPFFLYYASHHTHYPQFSGQSFPGHSGRGPFGDSLMELDAAVGALMTAVGDLGLLGETLVFFTADNGPETMRMSHGGCSGLLRCGKGTTFEGGVREPALAFWPGHIAPG-VTHELASSLDLLPTLAALAGAQLP-NITLDGVDLSPLLLGTGKSPR-HTLFFYSAYPDEVRGVFAVRSGKYKAHFFTQGSVHSDTTADPACHASNPLTAHEPPLLFDLSEDPGENYNLLDSVDEVAPEALQAVK 457          

BLAST of putative sulfatase vs. SwissProt
Match: gi|124007189|sp|Q571E4.2|GALNS_MOUSE (RecName: Full=N-acetylgalactosamine-6-sulfatase; AltName: Full=Chondroitinsulfatase; Short=Chondroitinase; AltName: Full=Galactose-6-sulfate sulfatase; AltName: Full=N-acetylgalactosamine-6-sulfate sulfatase; Short=GalNAc6S sulfatase; Flags: Precursor)

HSP 1 Score: 239.965 bits (611), Expect = 5.262e-72
Identity = 147/396 (37.12%), Postives = 205/396 (51.77%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPD-ETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLP------YSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENH--QDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGA-LAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHV 388
            PNI+L+  DD+G+GDLG  G    +TP LD +A +G     FY A+P+CSPSRA++LTG  P+R GF   +        P + +G  P+ E  +  +L  AGY    VGKWH G +P F P  HGFDEWFG P      Y N     + V       E+  R       F  ++P          +  +  +A+LT  Y +  +DFI+    +  PFFLY A    H P+Y   +F   S  G YG AV  ID +   I+  L  LG++ +T V FTSDNGA L    +  GSN P    K TT+EGG R P +  WPG + AG +  ++ + +DL  T  +  G +PP+DR+IDG D+   + L G     DR  FY     + AV +G++K H+
Sbjct:   28 PNIVLLLMDDMGWGDLGVNGEPSRETPNLDRMAAEGMLFPSFYSANPLCSPSRAALLTGRLPIRNGFYTTNAHARNAYTPQEIMGGIPNSEHLLPELLKKAGYTNKIVGKWHLGHRPQFHPLKHGFDEWFGSPNCHFGPYDNKAKPNIPVY---RDWEMVGR-------FYEEFP----------INRKTGEANLTQLYTQEALDFIQTQHARQSPFFLYWAIDATHAPVYASRQFLGTSLRGRYGDAVREIDDSVGKILSLLQNLGISKNTFVFFTSDNGAALISAPNEGGSNGPFLCGKQTTFEGGMREPAIAWWPGHIAAGQVSHQLGSIMDLFTTSLSLAGLKPPSDRVIDGLDLLPTM-LKGQM--MDRPIFYYRGNTLMAVTLGQYKAHL 400          

BLAST of putative sulfatase vs. SwissProt
Match: gi|1711564|sp|P15589.2|STS_RAT (RecName: Full=Steryl-sulfatase; AltName: Full=Arylsulfatase C; Short=ASC; AltName: Full=Steroid sulfatase; AltName: Full=Steryl-sulfate sulfohydrolase; Flags: Precursor)

HSP 1 Score: 241.121 bits (614), Expect = 6.229e-72
Identity = 170/438 (38.81%), Postives = 214/438 (48.86%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRV-LFPGQGVGLNPDETTMASILSDAGYATLAVGKWH----CGDQPDFL--PTNHGFDEWFGLPYSNDM-----GRQVLVEGQPSAEELNARI-----------RAAGIDFELKW------------------------PPLPLMEGTELVECQP-DQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGA----LAP-GFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLH 387
            PN +LI  DDLG GDLGCYG+    TP +D LA +G KLT    A+P+C+PSRA+ LTG YP+R G   AS   + V LF     GL P+E T A +L   GY T  VGKWH    C    DF   P  HGFD + G P +N       G  V    Q     L   I           R AG+     W                        P    +     +  QP D   LT R      DF+  ++D PF L+L+ M+VH   +    F   S +GAYG AV  +DWA   ++ TLD+LGLA++T+V  TSD+GA    L P G    GSN   R  K  TWEGG RVP LVRWPG +  G  V E T+ +D+ PT+A   GAE P DR+IDG D+  LL L     S     F+  +A + AV    W+ H
Sbjct:   26 PNFLLIMADDLGIGDLGCYGNRTLRTPHIDRLALEGVKLTQHLAAAPLCTPSRAAFLTGRYPVRSGM--ASHGRLGVFLFSASSGGLPPNEVTFAKLLKGQGYTTGLVGKWHLGLSCQAASDFCHHPGRHGFDRFLGTPTTNLRDCKPGGGTVFGSAQQVFVVLPMNILGAVLLAMALARWAGLARPPGWVFGVTVAAMAAVGGAYVAFLYHFRPANCFLMADFTITQQPTDYKGLTQRLASEAGDFLRRNRDTPFLLFLSFMHVHTAHFANPEFAGQSLHGAYGDAVEEMDWAVGQVLATLDKLGLANNTLVYLTSDHGAHVEELGPNGERHGGSNGIYRGGKANTWEGGIRVPGLVRWPGVIVPGQEVEEPTSNMDVFPTVARLAGAELPTDRVIDGRDLMPLL-LGHVQHSEHEFLFHYCNAYLSAV---AWRPH 457          

BLAST of putative sulfatase vs. SwissProt
Match: gi|341940244|sp|P50428.2|ARSA_MOUSE (RecName: Full=Arylsulfatase A; Short=ASA; AltName: Full=Cerebroside-sulfatase; Flags: Precursor)

HSP 1 Score: 238.81 bits (608), Expect = 1.056e-71
Identity = 167/440 (37.95%), Postives = 225/440 (51.14%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPD--FLPTNHGFDEWFGLPYSNDMGR-QVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDE--PFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFF--YIGDAN-IEAVRVGRWKLHV--------------------RKGGKEICELYDLVDDVGESIDVADD 414
            PNI+LI  DDLGYGDLG YG     TP LD LAE G + TDFY+   +C+PSRA++LTG  P+R G       +  VL P    GL  +E T+A +L+  GY T   GKWH G  P+  FLP + GF  + G+PYS+D G  Q L    P            G D  L   P+PL+    +    P    L  RYV    D + + Q +  PFFLY A  + H P +  + F + S  G +G ++  +D A   ++ T+  LGL ++T+VIFT+DNG         G +  LR  KGTT+EGG R P LV WPG +  G +  E+ ++LDLLPTLAA  GA  P +  +DG DI  LL   G +P     F+  Y  + + + AVR G++K H                     R    E   LYDL  D GE+ +V + 
Sbjct:   20 PNILLIFADDLGYGDLGSYGHPSSTTPNLDQLAEGGLRFTDFYVPVSLCTPSRAALLTGRLPVRSGM------YPGVLGPSSQGGLPLEEVTLAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPDIP------CKGGCDQGLV--PIPLLANLTVEAQPPWLPGLEARYVSFSRDLMADAQRQGRPFFLYYASHHTHYPQFSGQSFTKRSGRGPFGDSLMELDGAVGALMTTVGDLGLLEETLVIFTADNGPELMRMSNGGCSGLLRCGKGTTFEGGVREPALVYWPGHITPG-VTHELASSLDLLPTLAALTGAPLP-NVTLDGVDISPLLLGTGKSPRKSVFFYPPYPDEIHGVFAVRNGKYKAHFFTQGSAHSDTTSDPACHAANRLTAHEPPLLYDLSQDPGENYNVLES 443          

BLAST of putative sulfatase vs. SwissProt
Match: gi|135006|sp|P08842.2|STS_HUMAN (RecName: Full=Steryl-sulfatase; AltName: Full=Arylsulfatase C; Short=ASC; AltName: Full=Steroid sulfatase; AltName: Full=Steryl-sulfate sulfohydrolase; Flags: Precursor)

HSP 1 Score: 238.81 bits (608), Expect = 6.526e-71
Identity = 154/431 (35.73%), Postives = 216/431 (50.12%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWH----CGDQPDFL--PTNHGFDEWFGLPYSN--------------DMGRQVLVEGQP-------------------------SAEELNARIRAAGIDFELKWPPLP--LMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGAL-----APGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSF-FYIGDANIEAVR 380
            PNIIL+  DDLG GD GCYG+    TP +D LA  G KLT    ASP+C+PSRA+ +TG YP+R G    S   +  LF     GL  DE T A +L D GY+T  +GKWH    C  + DF   P +HGF+ ++G+  +N                 R V +  Q                          S   L A I    + F   + PL   +M   E+++      +LT R       FI+ + + PF L L++++VH  ++  + F   S++G YG AV  +DW+   I+  LD L LA+DT++ FTSD GA      + G    GSN   +  K   WEGG RVP ++RWP  + AG  + E T+ +D+ PT+A   GA  P DRIIDG D+  L  L+G +  +D  F F+  +A + AVR
Sbjct:   27 PNIILVMADDLGIGDPGCYGNKTIRTPNIDRLASGGVKLTQHLAASPLCTPSRAAFMTGRYPVRSGMASWSRTGV-FLFTASSGGLPTDEITFAKLLKDQGYSTALIGKWHLGMSCHSKTDFCHHPLHHGFNYFYGISLTNLRDCKPGEGSVFTTGFKRLVFLPLQIVGVTLLTLAALNCLGLLHVPLGVFFSLLFLAALILTLFLGFLHYFRPLNCFMMRNYEIIQQPMSYDNLTQRLTVEAAQFIQRNTETPFLLVLSYLHVHTALFSSKDFAGKSQHGVYGDAVEEMDWSVGQILNLLDELRLANDTLIYFTSDQGAHVEEVSSKGEIHGGSNGIYKGGKANNWEGGIRVPGILRWPRVIQAGQKIDEPTSNMDIFPTVAKLAGAPLPEDRIIDGRDLMPL--LEGKSQRSDHEFLFHYCNAYLNAVR 454          

BLAST of putative sulfatase vs. nr
Match: gi|750190441|ref|WP_040494515.1| (hypothetical protein [Ilumatobacter nonamiensis])

HSP 1 Score: 538.11 bits (1385), Expect = 0.000e+0
Identity = 281/483 (58.18%), Postives = 332/483 (68.74%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALA---PGFDGSG-----SNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHVRKGGKEICELYDLVDDVGESIDVADDHPDTVADLLARIEAGRHDLGDQAAGITGTGIRPIGRVDNPVTLTVVDPDDPETMAEYDLPYRG 477
            PN++LINCDDLGYGDLGCYGST +DTPALD LA  G +   FY ASPVCSP+R ++LTGCYP RIGFG  S   + VLFPG  +GL P E ++A +LSDAGYAT A+GKWHCGDQP F PTNHGFD + GLPYSNDMGRQ    G+P              +F  + PPLPL+   E+VE QPDQ SLT RYV   +DFI     +PFFLYLAHMYVH+PIYV++RF E S NGAYGAAVA+IDWAT VI++ LD  G+A++TIVIFTSDNG+L    P F GSG     SN PLR +KGTTWEGGQRVP ++RWP ++  G     + TA+DL PTLA  CGA+ P DR IDG DI  LL  D   PS   +FFY    ++EA+R GRWKLH  K G E   LYDL  D+GE+ D+ DDHPD VA L A  E  R  +GD   G  G+ +RPIGRVD+   LT  DP  P  +AEYDLP RG
Sbjct:    6 PNVVLINCDDLGYGDLGCYGSTRNDTPALDQLAADGIRFDSFYTASPVCSPARGALLTGCYPPRIGFG--SFHGLPVLFPGMDIGLPPTEISIAKVLSDAGYATQAIGKWHCGDQPHFQPTNHGFDHYLGLPYSNDMGRQA---GRP--------------EFMPEMPPLPLISDGEVVEQQPDQTSLTDRYVTEALDFIRRSNGQPFFLYLAHMYVHVPIYVQDRFAEASSNGAYGAAVAAIDWATSVILDELDARGIAENTIVIFTSDNGSLGDNPPPF-GSGEPLGASNDPLRGTKGTTWEGGQRVPGIIRWPKRIAPGRTSDAVLTAMDLFPTLATLCGADVPTDRTIDGHDISALL-FDDDVPSPHDAFFYYWMNDLEALRSGRWKLHFSKRGTEHRALYDLDTDIGETTDIIDDHPDVVARLDAVAETARATMGDARLGREGSDVRPIGRVDDAAPLTTYDPAHPYVVAEYDLPDRG 467          

BLAST of putative sulfatase vs. nr
Match: gi|1200244249|gb|OUT67677.1| (Cerebroside-sulfatase [bacterium TMED15])

HSP 1 Score: 526.554 bits (1355), Expect = 0.000e+0
Identity = 266/480 (55.42%), Postives = 328/480 (68.33%), Query Frame = 0

Query:    2 SPNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLH---VRKGGKEIC-ELYDLVDDVGESIDVADDHPDTVADLLARIEAGRHDLGDQAAGITGTGIRPIGRVDNPVTLTVVDPDDPETMAEYDLPYRG 477
             PNIILINCDDLGYGDLGCYGS ++ TP LD +A  G + TDFYM SPVCSPSR +MLTGCYP RIGF  A      VLFPGQ +GLNP E T+AS+L + GYAT  VGKWHCGDQ +FLPT HGFD ++G+PYSNDMGRQ   +                     K+PPLPL+   ++++ QPDQAS+T RYVE  V FI  +QD PFFLY AHMYVHLPIY  +RF   S NG YGAAV +IDW+  V+++ L +LGL ++T+VIFTSDNG+ A G    GSN PLR +KGTTWEGGQRVPC++RWP Q+P G++ +++ +A+DL PTL    G E P+DRIIDG DI  L+  +    +   +F Y    N+ A+R GRWKLH   V K G E+C ELYDLV D+GE+ ++ D HPD VADL A+++  R DLGD   G  G   R +GRVD+  TLT  DPD P  +A YDL  RG
Sbjct:    4 QPNIILINCDDLGYGDLGCYGSGINQTPYLDQMAADGIQFTDFYMGSPVCSPSRGAMLTGCYPPRIGF--AEFDGRWVLFPGQSLGLNPSEKTVASLLKEVGYATKIVGKWHCGDQKEFLPTRHGFDSYYGIPYSNDMGRQTPDD---------------------KYPPLPLLFDEDVIQEQPDQASVTERYVEESVRFIRANQDTPFFLYFAHMYVHLPIYPPDRFMRESENGRYGAAVQAIDWSVGVLLDELKQLGLDENTLVIFTSDNGSRARG--EGGSNKPLRGTKGTTWEGGQRVPCIMRWPSQIPPGSVSNQLVSAIDLCPTLVELAGGEIPSDRIIDGKDISSLIRNEEGAITPRETFLYYRLHNLMAIRSGRWKLHTHTVGKSGIEVCNELYDLVADIGETTNLYDQHPDIVADLNAQMDIYRKDLGDNLTGTKGENTRSVGRVDSSDTLTHYDPDHPYIIAMYDLKDRG 458          

BLAST of putative sulfatase vs. nr
Match: gi|1247506276|gb|PCJ62264.1| (Cerebroside-sulfatase [Planctomycetes bacterium])

HSP 1 Score: 524.628 bits (1350), Expect = 0.000e+0
Identity = 257/476 (53.99%), Postives = 329/476 (69.12%), Query Frame = 0

Query:    2 SPNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHVRKGGKEICELYDLVDDVGESIDVADDHPDTVADLLARIEAGRHDLGDQAAGITGTGIRPIGRVDNPVTLTVVDPDDPETMAEYDLPYRG 477
             PNIILINCDDLGYGDL CYGS V+ TPALD LA +G K TDFYM SPVCSPSR SMLTGCYP RIGFG + +    VLFPG  +GL+ DE T+A +L  A Y T  VGKWHCGDQ +FLP  HGFD ++G+PYSNDMGRQ            NA         + K PPLPL++  E+++ QPDQ  +T RY E CV FI +++D+PFFLYLAHMYVHLPIYV ++F + + NG YGAAV  IDW T V+++ L R G+ D+T+++FTSDNG+ A G    GSNAPLR  K  TWEGGQR+PC++RWP ++ +G +  E+T+++D   T AA  G E P DRIIDG DIR +L  +    S   +FFY    ++EAVR G+WKLHV +   ++ ELY+L  D+GE  ++ +  P+ V DL+ +I+A + D+GD+AAGI G  IRPIG+VD+P TLT  DP+ P  +A YDL  RG
Sbjct:    4 KPNIILINCDDLGYGDLACYGSKVNKTPALDKLAAEGIKFTDFYMTSPVCSPSRGSMLTGCYPPRIGFG-SFVNGGWVLFPGDALGLHEDEITIAKLLKQADYQTKIVGKWHCGDQKEFLPIRHGFDSYYGIPYSNDMGRQ------------NA---------DDKQPPLPLLKDEEVIQEQPDQRGITERYTEECVSFIRDNKDKPFFLYLAHMYVHLPIYVPKQFLDKAENGPYGAAVEHIDWVTAVLMDELKRQGIDDNTLIVFTSDNGSRAQG--EGGSNAPLRGRKTQTWEGGQRLPCIMRWPRKIKSGLVNKEITSSMDFYKTFAALAGVEIPTDRIIDGKDIRPMLFNEPDAKSQYDAFFYYKCNDLEAVRSGKWKLHVSRDKVKVEELYNLESDIGEQTNLFNQEPEIVKDLMIKIKACQKDIGDEAAGIKGENIRPIGKVDSPDTLTHYDPEHPYIIALYDLKERG 455          

BLAST of putative sulfatase vs. nr
Match: gi|505253058|ref|WP_015440160.1| (sulfatase [Ilumatobacter coccineus] >gi|464098030|dbj|BAN00912.1| putative sulfatase [Ilumatobacter coccineus YM16-304])

HSP 1 Score: 519.235 bits (1336), Expect = 4.207e-179
Identity = 274/487 (56.26%), Postives = 328/487 (67.35%), Query Frame = 0

Query:    2 SPNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALA----PGFDGS---GSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHVRKGGKEICELYDLVDDVGESI----DVADDHPDTVADLLARIEAGRHDLGDQAAGITGTGIRPIGRVDNPVTLTVVDPDDPETMAEYDLPYRG 477
             PN++LINCDDLGYGDLGCYGST++ TPA+D LA  G +   FY ASPVCSP+R ++LTGCYP RIGFG    + + VLFPG G+GL P+E ++AS+LSDAGY T AVGKWHCGDQPDF PTNHGFD +FGLPYSNDMG Q   E  P+  EL                PLPL+   +++E QPD A+LTGRYV+  + F+    + PFFLYLAHMYVHLPIYVEERF E S NG YGAAV SIDWAT VI+  L+  G+ DDTIVIFTSDNG+L     P  +G     SN  LR +K TTWEGGQRVP +VRWP +V AG +  E+ TA+DL PTLAA CG   P DRIIDG DI+ +   D P+P     F+Y    ++EAVR GRWKLH  K G E  ELYDL  D+GE+     DV  D PD V  L    E  R  LGD+  G +G+ +RP+G VD+P  LT  DPD P  +AEYD+  RG
Sbjct:    4 QPNVVLINCDDLGYGDLGCYGSTLNKTPAIDGLAADGLRFDSFYTASPVCSPARGALLTGCYPPRIGFG--RFEGVSVLFPGMGLGLAPEEYSLASLLSDAGYRTQAVGKWHCGDQPDFQPTNHGFDHYFGLPYSNDMGIQAGGE-PPTMPELA---------------PLPLIADGQVIEQQPDLAALTGRYVDEALKFMRVSDERPFFLYLAHMYVHLPIYVEERFAEQSSNGVYGAAVESIDWATAVILAELEAQGIVDDTIVIFTSDNGSLGDNPPPWGEGESLGASNGALRGAKTTTWEGGQRVPGIVRWPARVEAGRVSGELVTAMDLYPTLAALCGGRVPTDRIIDGRDIQSIWFGDAPSPH--EVFYYYWMNDLEAVRSGRWKLHFSKHGAEQRELYDLDSDIGETTNVLADVLADRPDVVERLDRLAEVARSTLGDERLGRSGSEVRPVGEVDDPAPLTRYDPDHPYIVAEYDITARG 470          

BLAST of putative sulfatase vs. nr
Match: gi|1052733719|emb|SCH43680.1| (Arylsulfatase [uncultured Ruminococcus sp.] >gi|1052733967|emb|SCI12300.1| Arylsulfatase [uncultured Clostridium sp.])

HSP 1 Score: 504.982 bits (1299), Expect = 7.773e-174
Identity = 254/470 (54.04%), Postives = 311/470 (66.17%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHVRKGGKEICELYDLVDDVGESIDVADDHPDTVADLLARIEAGRHDLGDQAAGITGTGIRPIGRVDNPVTLTVVDPDDPETMAEYD 472
            PNI+L+NCDDLGYGDLGCYGS V+ TP LD +A+ G + TDFYMASPVCSPSR  MLTGCYP RIGFG  + +   VLFPGQGVGLN  E T+A IL DAGY T  VGKWHCGDQ +FLP NHGFDE+FGLPYSNDMGRQ           LN               PLPL++G E++E QPDQ SLT RYVE+   FI  +++ PFFLY AHM+VHLP+Y  E F + S+NG YGA V  IDWA   ++  L + GL ++T+ IFTSDNG      D  GSN PLR  KGTTWEGGQRVPC++ WP  +  G  V ++ T++D LPT A  CG +   DR IDG D   +L  D  +P  +  F+YI +A +EAVRVG WKLH  K G+ +  LY+L  D GE+ ++ D++P+ VA L AR+E  RHDLGD   G  G  +RPIGRV+NP  L   + D P  +  YD
Sbjct:    5 PNIVLMNCDDLGYGDLGCYGSAVNRTPTLDDMAKHGIRFTDFYMASPVCSPSRGGMLTGCYPPRIGFG--TFEGEWVLFPGQGVGLNSSEVTIAEILRDAGYRTKIVGKWHCGDQEEFLPLNHGFDEYFGLPYSNDMGRQ-------KTSRLNT--------------PLPLIDGAEVIEEQPDQRSLTERYVEQATRFIRQNKENPFFLYFAHMHVHLPLYTSEEFVKNSQNGDYGACVECIDWAARAVLHELKKQGLEENTLFIFTSDNGGRG---DHGGSNRPLRGKKGTTWEGGQRVPCIMYWPNHIREGAEVHDLVTSMDFLPTFAKLCGGKVDPDRKIDGMDFSEMLFSDTASPR-NEFFYYIREA-LEAVRVGDWKLHTSKDGEAVKLLYNLRKDPGETNNLYDEYPEIVAKLEARLEYCRHDLGDTVTGTAGEKVRPIGRVENPKPLAEYNEDHPYIVMMYD 446          

BLAST of putative sulfatase vs. nr
Match: gi|1226760564|ref|WP_093990014.1| (Cerebroside-sulfatase [Massilimaliae timonensis])

HSP 1 Score: 503.056 bits (1294), Expect = 5.246e-173
Identity = 252/470 (53.62%), Postives = 311/470 (66.17%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHVRKGGKEICELYDLVDDVGESIDVADDHPDTVADLLARIEAGRHDLGDQAAGITGTGIRPIGRVDNPVTLTVVDPDDPETMAEYD 472
            PNI+L+NCDDLGYGDLGCYGS V+ TP LD +A+ G +  DFYMASPVCSPSR  MLTGCYP RIGFG  + +   VLFPGQGVGLN  E T+A IL DAGY T  VGKWHCGDQ +FLP NHGFDE+FGLPYSNDMGRQ           LN               PLPL++G+E++E QPDQ SLT RYVE+   FI  +++ PFFLY AHM+VHLP+Y  E F + S+NG YGA V  IDWA   ++  L + GL ++T+ IFTSDNG      D  GSN PLR  KGTTWEGGQRVPC++ WP  +  G  V ++ T++D LPT A  CG +   DR IDG +   +L  D  +P  +  F+YI +A +EAVRVG WKLH  K G+ +  LY+L  D GE+ ++ D++P+ VA L AR+E  RHDLGD   G  G  +RPIGRV+NP  L   + D P  +  YD
Sbjct:    5 PNIVLMNCDDLGYGDLGCYGSAVNRTPTLDDMAKHGIRFADFYMASPVCSPSRGGMLTGCYPPRIGFG--TFEGEWVLFPGQGVGLNSSEVTIAEILRDAGYRTKIVGKWHCGDQEEFLPLNHGFDEYFGLPYSNDMGRQ-------KTSRLNT--------------PLPLIDGSEVIEEQPDQRSLTERYVEQATRFIRQNKENPFFLYFAHMHVHLPLYTSEEFVKNSQNGDYGACVECIDWAARAVLHELKKQGLEENTLFIFTSDNGGRG---DHGGSNRPLRGKKGTTWEGGQRVPCIMYWPNHIREGAEVHDLVTSMDFLPTFAKLCGGKVDPDRKIDGMNFSEMLFSDTASPR-NEFFYYIREA-LEAVRVGDWKLHTSKDGEAVKLLYNLRKDPGETNNLYDEYPEIVAKLEARLEYCRHDLGDTVTGTAGENVRPIGRVENPKPLAEYNEDHPYIVMMYD 446          

BLAST of putative sulfatase vs. nr
Match: gi|949038892|gb|KRO43264.1| (Cerebroside-sulfatase [Acidimicrobium sp. BACL17 MAG-120823-bin42])

HSP 1 Score: 500.745 bits (1288), Expect = 7.894e-172
Identity = 260/484 (53.72%), Postives = 317/484 (65.50%), Query Frame = 0

Query:    2 SPNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALA---PGFDGS-----GSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHVRKGGKEICELYDLVDDVGESIDVADDHPDTVADLLARIEAGRHDLGDQAAGITGTGIRPIGRVDNPVTLTVVDPDDPETMAEYDLPYRG 477
             PNI+ INCDDLGYGDLGCYGS+++ TPALD LA +G + T  YM SPVCSPSRA++LTGCYP RI FG     H  VLFPGQG+GL P E ++A +LS+AGY T  +GKWHCGDQP FLPTNHGFD +FGLPYSNDMGRQ       S E L         D              +++E QPDQ+ LT RYV+  +DFI     +PFFLYLAHMYVHLPIYV++ F + S NG+YGAAV SIDWAT VI+  L  LGL ++TIVIFTSDNG+L    P F        GSN PLR +KGTTWEGGQRVP +VRW G++ AG +  E+ TA+D  PTLA  CGA+ P DRIIDG D + +   D    S    F Y     +EA+R  ++KLH  +G +++ ELYD+  DV E+++V D +P+ VA L       R  LGD   G  GT +RPIGRV+NP  LT  D   P  +AEYDLP RG
Sbjct:    3 QPNIVFINCDDLGYGDLGCYGSSLNKTPALDQLANEGIRFTSLYMGSPVCSPSRAALLTGCYPPRISFGEFDGWH--VLFPGQGLGLPPTEISIAKVLSEAGYRTQMIGKWHCGDQPGFLPTNHGFDHYFGLPYSNDMGRQ-----SNSPEWLVLPPLPLLHD-------------DDVIEQQPDQSLLTERYVQEAIDFIRTDSPKPFFLYLAHMYVHLPIYVKQEFLDKSENGSYGAAVESIDWATSVIMNELHSLGLDENTIVIFTSDNGSLGNVHPPFGDRKELIGGSNLPLRGAKGTTWEGGQRVPAIVRWKGKIEAGRVCDELLTAMDFYPTLANLCGAKVPQDRIIDGIDAQAVW-FDAQAKSQHEHFVYYRANVLEAIRDRQYKLHFGRGNQDVFELYDIAHDVSETVNVYDRYPEVVASLTKAANNYRQTLGDDRTGSVGTQVRPIGRVENPRPLTEFDSRHPYVIAEYDLPDRG 465          

BLAST of putative sulfatase vs. nr
Match: gi|506364739|ref|WP_015884458.1| (hypothetical protein [Beutenbergia cavernae] >gi|229568370|gb|ACQ82221.1| Cerebroside-sulfatase [Beutenbergia cavernae DSM 12333])

HSP 1 Score: 496.508 bits (1277), Expect = 2.944e-170
Identity = 267/474 (56.33%), Postives = 316/474 (66.67%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPAD--RIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHVRKGGKEICELYDLVDDVGESIDVADDHPDTVADLLARIEAGRHDLGDQAAGITGTGIRPIGRVDNPVTLTVVDPDDPETMAEYDLP 474
            PNI+LIN DDLGYGDLGCYGS  +DTP LD LA +G +LTDFYMASPVCSPSR  MLTGCYP RIGFG        VLFPG  VGL+P E TMA +L DAGYAT A+GKWHCGDQP+FLPT HGFD +FG+P+SNDMGRQ   E                      WPPLPLM G  +V+ QPDQ SLT RY      FIE +  +PFFLYLAHMYVH+P++V   F   SRNG YG AVA++DW+T V+++TL RLGL ++TIV+FTSDNG+ A G    GSN PLR  K  TWEGGQRV C+VRWP  +PAG +   +T ++DLLPT AA  GA   AD  R +DG D+  LL   GP P+   +++Y+ D  +EAVRVG WKLH+ K    + ELYDL  D  E+ DVA DHPD VA L A  E  R DLGD   G+ G G RP GRV++   LT    D P  +A YDLP
Sbjct:    5 PNIVLINADDLGYGDLGCYGSMRNDTPHLDRLAAEGVRLTDFYMASPVCSPSRGGMLTGCYPPRIGFG--EFVGRPVLFPGDPVGLDPAERTMARVLGDAGYATAAIGKWHCGDQPEFLPTRHGFDSYFGIPFSNDMGRQREHE---------------------DWPPLPLMSGESVVQEQPDQRSLTERYTVAATRFIEENAHQPFFLYLAHMYVHVPLFVPAPFLAASRNGGYGGAVAALDWSTGVVMDTLRRLGLEENTIVVFTSDNGSRARG--EGGSNDPLRGHKAQTWEGGQRVACVVRWPAAIPAGGVCDAVTRSIDLLPTFAAVAGAADWADPARPVDGVDLTALLTGAGPAPNETFAYYYMDD--LEAVRVGDWKLHLSKRRDPMRELYDLRTDAAETHDVAADHPDVVARLEAVAETIRADLGDARLGVVGAGRRPQGRVEDAQPLTSYREDHPYLVAMYDLP 451          

BLAST of putative sulfatase vs. nr
Match: gi|496544072|ref|WP_009250147.1| (MULTISPECIES: hypothetical protein [Clostridiales] >gi|336009236|gb|EGN39231.1| hypothetical protein HMPREF0994_00198 [Lachnospiraceae bacterium 3_1_57FAA_CT1])

HSP 1 Score: 494.582 bits (1272), Expect = 1.316e-169
Identity = 258/470 (54.89%), Postives = 308/470 (65.53%), Query Frame = 0

Query:    3 PNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHVRKGGKEICELYDLVDDVGESIDVADDHPDTVADLLARIEAGRHDLGDQAAGITGTGIRPIGRVDNPVTLTVVDPDDPETMAEYD 472
            PNIILINCDDLGYGD+GCYGS V+ TP +D LA+ G + T FYMASPVCSPSR +M+TGCYP RIGF   S +   VLFPGQGVGLN DE T+A IL  AGY T  VGKWHCGDQ +FLPTNHGFDE++GLPYSNDMGRQV  E                 DF    PPLPL+ GT +VE QPDQ S+T RYVE+C  FI  +++  FFLYLAHM+VHLP+Y  E F + S NG +GA V +IDW+  V++  L  LGL +DT+VIFTSDNG      D  GSNAPL+ +KGTTWEGGQRVPC+  WPG +  G    E+  +LD   TLA       PADR  D  DIR ++   G TP    +FFY    N+EAVR   WKLHV K GK +  LY + +D GE  +V + HP+ V  L   +E  R+ LGD  AGI GT IRPIGRV+NP  L   D + P  +  YD
Sbjct:    8 PNIILINCDDLGYGDVGCYGSGVNRTPGIDYLAQNGMRCTSFYMASPVCSPSRGAMMTGCYPPRIGF--ESFEGEWVLFPGQGVGLNSDEVTIAQILQRAGYRTKLVGKWHCGDQEEFLPTNHGFDEYYGLPYSNDMGRQVFKE-----------------DF----PPLPLLSGTGIVELQPDQRSITERYVEQCRTFIRENKNNKFFLYLAHMHVHLPLYAGEEFVKKSINGDFGACVEAIDWSVRVLLHELRSLGLEEDTLVIFTSDNGGRG---DHGGSNAPLKGNKGTTWEGGQRVPCIFYWPGHIAPGQECRELICSLDFYRTLAELGHGTMPADRKNDSLDIRRVILEGGKTPRD--TFFYYIMGNLEAVRDKEWKLHVAKDGKPVRLLYHISEDPGEQENVYESHPEIVKHLEHSLEECRYKLGDTLAGIKGTQIRPIGRVENPHPLAEYDINHPYVVMMYD 449          

BLAST of putative sulfatase vs. nr
Match: gi|1085384510|gb|OGV55010.1| (Cerebroside-sulfatase [Lentisphaerae bacterium GWF2_44_16])

HSP 1 Score: 493.812 bits (1270), Expect = 2.856e-169
Identity = 250/472 (52.97%), Postives = 311/472 (65.89%), Query Frame = 0

Query:    2 SPNIILINCDDLGYGDLGCYGSTVHDTPALDALAEQGAKLTDFYMASPVCSPSRASMLTGCYPLRIGFGGASMQHMRVLFPGQGVGLNPDETTMASILSDAGYATLAVGKWHCGDQPDFLPTNHGFDEWFGLPYSNDMGRQVLVEGQPSAEELNARIRAAGIDFELKWPPLPLMEGTELVECQPDQASLTGRYVERCVDFIENHQDEPFFLYLAHMYVHLPIYVEERFHEGSRNGAYGAAVASIDWATEVIIETLDRLGLADDTIVIFTSDNGALAPGFDGSGSNAPLRASKGTTWEGGQRVPCLVRWPGQVPAGTIVSEMTTALDLLPTLAAACGAEPPADRIIDGDDIRGLLGLDGPTPSADRSFFYIGDANIEAVRVGRWKLHVRKGGKEICELYDLVDDVGESIDVADDHPDTVADLLARIEAGRHDLGDQAAGITGTGIRPIGRVDNPVTLTVVDPDDPETMAEYDL 473
             PNIILINCDDLGYGDL CYGS  + TP LD +A +G + TDFYMASPVCSPSR +M+TGCYP RIGFG        VL  G G+GLNP ETT+AS+L   GYAT  VGKWHCGDQP+FLPT HGFDE++GLPYSNDMGRQ            N R              LPL+   E+++ QPD ++LT RYVE+CV FI N+++ PFFLY AHM+VH PIY  + F E S NG YGA VA +DW+T VI   L +LG+ DDT+V+FTSDNG+ A G    GSNAPLR +K TTWEGG RVPC++RWPG + AG   SE+ +++D   T A   G   P DRI D  DI  LL L+    S   +FFY    ++EAVR   WKLH+RK G++  ELY+L +D+ E+ ++ ++HPD V  L   +++ R+D GD+A GI G   RP G V NP  LT  +P+ P   A YDL
Sbjct:    3 KPNIILINCDDLGYGDLNCYGSEHNKTPYLDKMASEGIRFTDFYMASPVCSPSRGAMMTGCYPPRIGFGSFDTP-CPVLTAGSGLGLNPKETTIASLLKKTGYATKLVGKWHCGDQPEFLPTRHGFDEYYGLPYSNDMGRQ------------NGRTNNPPP--------LPLLLNEEVIQEQPDLSALTERYVEQCVSFIRNNRNSPFFLYFAHMHVHRPIYAPQSFLEESENGRYGAGVAQVDWSTGVIFSELKKLGIDDDTLVVFTSDNGSRASG--EGGSNAPLRGTKRTTWEGGMRVPCIMRWPGHIEAGQTCSEIVSSMDFYATFANLTGVSVPDDRITDSLDISSLL-LEKNKRSPRDTFFYYYKNDLEAVRHDEWKLHIRKHGEKTIELYNLKEDISETKNIYNEHPDIVKKLETLLQSARNDTGDEALGIKGANCRPPGIVKNPRALTEYNPEHPYFQAMYDL 450