BLAST of putative restin vs. L. salmonis genes
Match: EMLSAG00000006131 (supercontig:LSalAtl2s:LSalAtl2s334:31459:138645:-1 gene:EMLSAG00000006131 transcript:EMLSAT00000006131 description:"maker-LSalAtl2s334-augustus-gene-1.5")

HSP 1 Score: 548.125 bits (1411), Expect = 3.339e-163
Identity = 308/693 (44.44%), Postives = 458/693 (66.09%), Query Frame = 0

Query: 1087 QMEDLQGELKMRGSTSEETEKALKDEKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEIDLADKTEELELAEKSI----KDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFNDLNTDLEVKEASFDNSFKETDEKHKDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILDLKASLEELEGRVTSLNSSSADEGKIHEAKVAKITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVDELQIALAEAETERDSARSKSKDFQKISTQNVTLDEDNENLKKDIEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRSGRKSPSPAKSISSDMN----------DGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGII-----DSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            ++EDLQ EL    +  +   K L  E   R+EL+E +E L  +H+EE+ ELVDVVN+L+ K+ +L     +LE+   ++    +D  +K   + +ELD  K +   +I VL +SLE++ +QI +LKES   +KVEYDL+ +K K + +  + +   EMDKK +LI+ +K ++++ E E E   EEL+AKIEE   ++ DLEVKEASF N  +  DE H  E+E+LV+K+E+A+ E+ DL+E LA+T+A+HDSK+DE+   KK EVN+LQD +SERDR I+D KA + ELE ++T +  +S DE  +  AKV K +S+ E+LR  +++L+S +K+++   D++S QNE+L++K+VE+E QV E TSQ QT+KVS+E+++KEL E  EKS +      EL  A++ ER RVDELQ+AL E E+ERD    + +DF     +   ++     L   IEELK +    QD F +ER E+NEVV+ S +LL +K+EEFKS K+E + L  EN Q+NS++R ++ILEQEKRE+E+++  L   +  G+  P   K  ++ +N          +  L++Q++FLNSVIVDMQRKND  K KLE++ES  ++     D  ++ N + NG+++  V PRLFCDICD FD+HDTEDCPTQAM +     HT++  +R   R YC++CEVFGH+T +CN+ ETF
Sbjct: 1452 ELEDLQNELVESKAKLQSVSKELSKESKFRVELQEKLESLHSTHDEEMGELVDVVNDLKEKDDELKVAMSQLEIKADTLVKVERDAERKEKLYKEELDRIKVECQDKIQVLTQSLEDTNAQISYLKESSASSKVEYDLRKEKLKTEKQQLEDEHNKEMDKKDELIQRLKSELSSNEVEQEVINEELKAKIEELEQVSMDLEVKEASFSNKVRINDESHNSEVEILVSKVEDAKIEIIDLNEKLARTKADHDSKIDELKLEKKVEVNNLQDLLSERDRDIVDHKAKISELENQITDIIRTSEDEVNLLNAKVVKFSSEFEELRTIRRELDSRVKTIETQRDEMSMQNEELNVKIVEKERQVNECTSQFQTIKVSFERTKKELAEHIEKSIEISSGNDELVTAINMERKRVDELQVALGETESERDXLSQQLEDFXATKERLRIVEGSEHELNGMIEELKRQSASMQDKFSAERKEMNEVVENSHILLTEKIEEFKSLKKESELLHSENAQINSFKRNILILEQEKREMENKLNLLGTNNVMGK--PDIMKKSTTTVNIIDNEDEEESNENLRSQINFLNSVIVDMQRKNDKYKMKLELYESGKVLGGDMDDENSQLNLLLNGINANQVPPRLFCDICDQFDLHDTEDCPTQAMMVVXEGSHTRNTASRSSNRPYCESCEVFGHATQDCNDEETF 2142          

HSP 2 Score: 296.59 bits (758), Expect = 4.621e-81
Identity = 175/379 (46.17%), Postives = 232/379 (61.21%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLS-RQPLAS---GPPSCLTTPR--------KVSKAGRAS-----MSPS----ESVV----DLKSGTPSPTGSTISLVSM----APLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQ-RQPSSL------TP-RKLNRERSDLSEASMASMMSTVSRSQVPVKKKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEE 349
            KV V G   G I+FLGETKFA G WAGV LD   GKNDG+V GVSYFKC PM+GVFSR SRLS R  L +   GP   +  P+        KVS     S      SP+     S++    D    TPSPTGS IS+ ++    +P +LGDRVIV +  + +K+GTLR++G  EFAQG+W GVELD   GKNDG V    YFEC PN G+F    K+TKSP++ MQ RQ S+L      TP R++ R+RSDLS  S  S  +         K++S++SI APE LK+SLKEKE  I+++  E E ERAQ +KAA+QTD+ E  L ++  E+G++K E         ++  K +E+I  L +KL +EK+K +D++F+ EE
Sbjct:  609 KVCVRGNTPGVISFLGETKFASGLWAGVVLDKPEGKNDGSVNGVSYFKCKPMYGVFSRPSRLSLRDALENTRKGPIPNINFPQLNLPYSKEKVSVQSLNSQLCLGTSPTKTLQHSIISSFDDPTQRTPSPTGSQISISTLKSSESP-QLGDRVIVSSTTSNSKVGTLRYLGKAEFAQGEWAGVELDLPYGKNDGTVQGVAYFECKPNHGVFVLSSKVTKSPRSFMQFRQISNLSSASIATPNRRICRQRSDLSNISATSTSAG--------KRRSVSSINAPEMLKLSLKEKEAEIEKITKEMELERAQFSKAANQTDILESKLKEMEREFGTFKLES-------ISSEHKKDERISELSSKLSEEKQKFEDLQFQFEE 971          

HSP 3 Score: 154.451 bits (389), Expect = 1.418e-37
Identity = 255/983 (25.94%), Postives = 447/983 (45.47%), Query Frame = 0

Query:  444 KEVDSLKTRLVATE---DKLKEARTHLDVVKEESGNRV-----------FESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLKQDLKDQLTKAESEIRSLKEELDGAKTKVEGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQLDKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQDQLEQIQKDAKKTTHELEEKLKKSEATKEKAEKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKE-------KIKLEEAIHAMQSTSVDSDALIGNL----------NDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLEL---DLETTQKDNAVLKENSILAKAQFDDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKDEKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEIDLADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMD--KFKKQIEA----------------QKSKFEDEMDKKTDLIKSMKDDIT-------------------------------NKEEELEFAKEELEAKIEE----FNDLNTDLEVKEASFDNSFKETDEKHKDEIEV------LVTKIEEARAEVADLSETLAKTRAEHD 1333
            KE+  LK  LV +E   +KLKE   +   V+ ES   +           FE+EE+ + L  EVD L+K+L+  K SLERE +   ++Q  +K +      +I  LE+ +S K++ +LE   K++S +          E+   ELKE+    Q+L  + ++ E E++    E+D    K+ GL+  +  +D  IKS++   T+  K      +E ++  + L +  S T + + +   +   L  KL++   ++ +    +E    K  R + +  EA ++  +V     +LEKEI   +  S  + +EVKRLSG I+ KD EL  LRGD++ L S+L  LK VI Q+E E +++   + K+ +K   +L E LKKSE   +K EK K +AI   + K+     +                     L  + +EL+  ++ T  E+++ LTE             D ER+        QT + T  +L S+KE++E             +D   +L  S+ KL  ++KE       +++L+E + ++ ST    D  +G L          +DELK   +++E ++ +   KVE+  +  + L KE+  +    +K+   +DK+++    LE T    + LKE+S  +K ++D  L + KL+ +             K Q+ED           ++E +K  KDE  +R++ E +          EVE+  +V+NE      +L  K EELE       DL  K      ++ +  E H+ E+++L   +E++K +I+ L E +   K ++D ++D  K +K++E                  K+K  +  ++ TD+I++ +D++                                 + +E+    EEL  KI E     N+  +  +  + SF+ + KE  E  +  IE+      LVT I   R  V +L   L +T +E D
Sbjct:  985 KEISLLKKELVESESTIEKLKEVEIYFKKVQSESKTMIKDLEEKCSVLAFEAEEKTVALNLEVDELKKNLESYKISLERERERSISVQGKMKTEEESVTRKIETLEEYISKKEDEILEMNMKIESFEKSEQCTFEKYEKALSELKESDLKNQELTTKTSQMEVELKCQYGEVDRLTNKINGLNEVMELKDTEIKSVKKILTQTQKSLKSTKEESEKAMAXLSEXDSSTNIEISKLMGENAALREKLDEDSENMNSSNKLVESIKEKLERTELEFIEANKKFENVCSINRNLEKEIETLRSGSSINNEEVKRLSGXISLKDSELSSLRGDVNILKSQLNTLKDVITQTEFEAKEKEALLIKEQEKDIFKLMEALKKSEGLLKKMEKTKADAIDACNSKMNAARKE---------------------LNEQINELKVEMMETKVENTR-LTE-------------DLERSR---VHATQTGSETSEELSSLKEAIESNIKMTKFKEQELEDLQNELVESKAKLQSVSKELSKESKFRVELQEKLESLHST---HDEEMGELVDVVNDLKEKDDELKVAMSQLE-IKADTLVKVERDAERKEKLYKEELDR----IKV-ECQDKIQVLTQSLEDTNAQISYLKESSASSKVEYD--LRKEKLKTE-------------KQQLED---------EHNKEMDK--KDELIQRLKSELS--------SNEVEQ--EVINE------ELKAKIEELEQVS---MDLEVKEASFSNKVRINDESHNSEVEILVSKVEDAKIEIIDLNEKLARTKADHDSKIDELKLEKKVEVNNLQDLLSERDRDIVDHKAKISELENQITDIIRTSEDEVNLLNAKVVKFSSEFEELRTIRRELDSRVKTIETQRDEMSMQNEELNVKIVEKERQVNECTSQFQTIKVSFERTKKELAEHIEKSIEISSGNDELVTAINMERKRVDELQVALGETESERD 1875          

HSP 4 Score: 144.436 bits (363), Expect = 1.496e-34
Identity = 74/191 (38.74%), Postives = 104/191 (54.45%), Query Frame = 0

Query:    4 LLLLKVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPPSCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISL--VSMAP--LKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFA 190
            ++ + V+V GTK G+IA++G+  FA G  AG  LD    KN+G++GG+  F+C P  G+FS+  RL+R  + S  P          K  R +   + S    K G     GS +S+  VS  P  LK+GD+V     V G   G + F+G T+FA G W GV LD   GKNDG V    YF+C P +G+F+
Sbjct:  494 MVGMPVYVDGTKPGRIAYIGDVHFAQGIMAGXHLDXPQXKNNGSIGGIMXFQCEPKRGIFSQLHRLTRIFIMSSIP----------KPSRIARPLTTSQTKEKGG-----GSNVSISEVSSNPLNLKVGDKV----CVRGNTPGVISFLGETKFASGLWAGVVLDKPEGKNDGSVNGVSYFKCKPMYGVFS 665          

HSP 5 Score: 109.383 bits (272), Expect = 7.409e-24
Identity = 41/66 (62.12%), Postives = 52/66 (78.79%), Query Frame = 0

Query:    9 VWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPL 74
            V+V G + G+I F+GETKF  GEWAGV L++ALGKNDG VG V YF+C P HGVF+R  RL+R+P+
Sbjct:   91 VYVDGVQRGRIQFIGETKFGHGEWAGVFLEEALGKNDGAVGNVRYFQCEPRHGVFARLFRLTREPI 156          

HSP 6 Score: 88.9669 bits (219), Expect = 1.098e-17
Identity = 34/73 (46.58%), Postives = 49/73 (67.12%), Query Frame = 0

Query:  129 DRVIVGNAVT--GTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSP 199
            D  I+G  V   G + G ++F+G T+F  G+W GV L+ A+GKNDG VG+ RYF+C P  G+FA L ++T+ P
Sbjct:   83 DSFIIGETVYVDGVQRGRIQFIGETKFGHGEWAGVFLEEALGKNDGAVGNVRYFQCEPRHGVFARLFRLTREP 155          

HSP 7 Score: 63.5438 bits (153), Expect = 5.394e-10
Identity = 27/69 (39.13%), Postives = 40/69 (57.97%), Query Frame = 0

Query:  131 VIVGNAVT--GTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITK 197
            ++VG  V   GTK G + ++G   FAQG   G  LD    KN+G +G    F+C P  G+F+ LH++T+
Sbjct:  493 LMVGMPVYVDGTKPGRIAYIGDVHFAQGIMAGXHLDXPQXKNNGSIGGIMXFQCEPKRGIFSQLHRLTR 561          

BLAST of putative restin vs. L. salmonis genes
Match: EMLSAG00000000550 (supercontig:LSalAtl2s:LSalAtl2s108:36082:56433:-1 gene:EMLSAG00000000550 transcript:EMLSAT00000000550 description:"augustus_masked-LSalAtl2s108-processed-gene-0.0")

HSP 1 Score: 83.9593 bits (206), Expect = 2.110e-16
Identity = 58/187 (31.02%), Postives = 90/187 (48.13%), Query Frame = 0

Query:   15 KSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVF---SRASRLSRQPLASGPPSCLTTPRKVSKAGRASMSPSESV----VDLKSGTPSPTGSTISLVSMAPLKLGDRV----IVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFA 190
            K G + + G T FAPG W GV LD+  GKNDG+V GV YFKC   HG+F    + S++ R  ++ G    +  P   S+   + ++ ++ V    VD+   +   T +   +     +K GDRV    ++   +  ++                W GVELD A+GK+DG V   +YF    + G+F 
Sbjct:  317 KFGSLEYCGLTSFAPGVWVGVKLDELHGKNDGSVQGVVYFKCQKNHGLFVPPGKISKMGRTYVSKG----IKDPSITSQISNSPINKTKVVNRGRVDVSKVSSKLTNAIEEIAERKEIKEGDRVREEKLISLCIKYSR----------------WYGVELDEALGKHDGTVRGVKYFPSKADHGVFV 483          

HSP 2 Score: 71.2478 bits (173), Expect = 1.947e-12
Identity = 46/114 (40.35%), Postives = 62/114 (54.39%), Query Frame = 0

Query:  125 LKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQ---PS-----SLTP----RKLNRERSDLSEAS 226
            LK+GDRV++       K G+L + G T FA G W GV+LD   GKNDG V    YF+C  N GLF P  KI+K  +  + +    PS     S +P    + +NR R D+S+ S
Sbjct:  303 LKVGDRVVLRGE--DEKFGSLEYCGLTSFAPGVWVGVKLDELHGKNDGSVQGVVYFKCQKNHGLFVPPGKISKMGRTYVSKGIKDPSITSQISNSPINKTKVVNRGRVDVSKVS 414          

HSP 3 Score: 55.4546 bits (132), Expect = 1.239e-7
Identity = 28/64 (43.75%), Postives = 38/64 (59.38%), Query Frame = 0

Query:  125 LKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGL 188
            L++G   I    V   +MG +RF+G   FA G W G+EL S  GK+DG+V +KRYF    N G+
Sbjct:  618 LEVGTTAI---CVHNKEMGIVRFVGRVHFASGIWVGLELRSPNGKHDGIVEEKRYFTAKTNHGV 678          

BLAST of putative restin vs. L. salmonis genes
Match: EMLSAG00000002033 (supercontig:LSalAtl2s:LSalAtl2s136:1281404:1312976:1 gene:EMLSAG00000002033 transcript:EMLSAT00000002033 description:"maker-LSalAtl2s136-augustus-gene-13.17")

HSP 1 Score: 76.2554 bits (186), Expect = 9.235e-14
Identity = 195/960 (20.31%), Postives = 398/960 (41.46%), Query Frame = 0

Query:  191 PLHKITKSPKARMQRQPSSLTPR-KLNRERSDLSE------ASMASMMSTVSRSQVPVKKKSIASIMAPERLKMSLKEKE-DRIDQLLMEREQERAQIAKA-ASQTDVAEGNLSKIIE--------EYGSYKREQEERALT-YRATMEKYEEKIRSLKNKLED--EKRKH-------------DDVEFRLEEETMMRSESD----------AGNSGELIEKIRSLEDQL--------AQELKAKADLNGGKSAFETRIADLEGKLGSEE-----------KMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRLVATEDKLKEARTHLDVV---------KEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKA------LSDKDNLLLESQHKLDS------------------LQSGSSSVQSTLEEKSRELKENL---------------KLKQDL---KDQL-----TKAESEIRSLKEELDGAKTKVEGLSNQVGQR-DETIKSLEGQKTKIHKQFGVKSKEIDELKSQLDKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEK---EIRLFKDSSQTSTDEVKR-LSGT--INSKDLE--LEMLRGDLSSLTSKLEELKLVIDQ---SETEHQDQLEQIQKDAKKTTHELEEKLKKSEATKEKAEKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTK-DFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSD----ALIGNLNDELKANKAEVERVREELEQKVEQHQKDLDDLAK 1015
            P+    K+ + + Q    SL    ++ + RS+++E       S + +    S  + P+++K   +   PER   +L  K  D I ++L++ +   +   K  A  T +  G LS I +        ++G++  + E   ++ +  T ++ +++I   K +L    EK  H             +D+ F+L+ +T + S++            G  G L E+I +  +Q+          E K K+  +  K A  T++    G+L S +           +   D     ++  + +D L ++ E ++  +  +    + LK      +D++K  R  ++ V         K    N +  + ++ + L++ +  + K  +E+  +++  E++   + + +K  +  S+ + SELE A      L   D+LLL S +KL +                  L S   SV++  EE+ +E K N+               K++++L   KD L     +K E    SLK+E++  K ++    N+V     + IK LE +K  I+  F   +K+I+ +  ++     D     ++      E +  L   +   +++K ++ ++   + +  +   + M + S +K  ++D+ K   +I+   + +Q   ++V   LSGT  ++ KDL+   + L  D+ S + +   +K   D    SE      L++ QKD      ++   + ++           EE I ++   +   +N  ++L   + G +++     E L  K  E      S+   SSK +  L  K  +  ++    ++ ++ L    + +   + D+E I + LE    +    + T K  F   L  SQ K+S       KL++ +  +    V S      LI N  + + AN   V        Q V ++QK +D + K
Sbjct:   33 PITPFKKADQKKSQMYSLSLEENSEIAQLRSEVAELRQIINESTSGINRVPSSPRPPIRRKQSNT---PERTSAALDSKSLDEISKILLDHDVCISDQKKTLARMTKLCLGALSAIAKLENKILKSDFGNHCSDSESNLISIWENTYKQLKKEIIETKTELSSIAEKVSHCPNGNIGIGSEVSEDI-FKLKNQTQILSDNILRTDKSVIGFQGQLGTLQEQIGNCNEQIKMHLSKKVGSEQKFKSSYDNNKEAI-TKMDLTIGRLISNDLPNLTIRLKDCESTIDKQVSSAEHDVRIDALHKEFEKKY--DSLKSDLFNELKNCENVYDDQIKVLREGIECVVSKTEGYIDKNRFENLIENTRKETVELRENMGKIIKQDEENTKNIKSLEQSSTTMAKEIKNCTELSLKKASELENAQKKMDELKKGDDLLLNSVNKLQNQVNGIVKEIKDCQTKGKELSSNLESVKNVFEEERKEEKLNISKLETSLGKTLKSQEKIEKELSSNKDNLAEQVRSKVEFCSDSLKKEINFCKKELNCCRNEVSDMGKDIIKHLEDEKLHINSSFKPFNKDIETMNHKITSCLGDMNKWNKKLDTFHQEFTLGLNKVKDDSISVKKEVAIDFEASLQENSVCLQKMID-SFMKSTEQDINKHNQKIKEIDNENQIMKNKVSEALSGTTFVSKKDLKDIQKNLINDIKSASEQYSGIKADFDDFKLSERSKYTLLDKKQKDINTFCEDINATIYRT-----------EEDIKDIRLDVSVDSNTINELKMKISG-INESKDKFEHLSRKVEEYLAQFKSSIQTSSKGVNALEDKYANEFLSV---KKRIDGLVTSKKIRDKLVKDVERIDKKLEQYHEKVMDKIDTNKYSFENDLQLSQEKISLSENSIQKLDKRMDELHENEVKSRENIAPLICNFKEAMDANFTYV-------IQHVMENQKFIDSINK 962          

HSP 2 Score: 65.4698 bits (158), Expect = 1.467e-10
Identity = 194/1023 (18.96%), Postives = 408/1023 (39.88%), Query Frame = 0

Query:  479 FESEEQIIMLKDEVDTLRKDLDESKGSLEREEKN----MAALQESLKEQSTKSIDQ--ISELEKALSDKDNLLLESQHKLDSLQS---GSSSVQSTLEEKSRELKENLKLKQDLKDQLTKAESEI--------RSLKEELDGAKTKVEGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQLDKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQ--SETEHQDQLEQIQKDAKKTTHELEEKLKKSEATKEKAEKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNLND------ELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQFDDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKM-RGSTSEETEKALKDEKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEIDLADKTEELELAEKSIKDLNKKLGQH----------------HKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKF--KKQIEAQKSKFEDEMDKKTDLIKSMKDD--------------ITNKEEELEFAKEELEAKI----EEFNDLNTDLEVKEASFDNSFKETD--EKHKDEIEVLVTKIEEARAEVADLSETLAK-TRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILDLKASLEELEGRVTSLNSSSADEGKIHEAKVAKITSDN----EDLRLKKKDL---ESTLKSLQADFDQLSNQNE 1429
             E   +I  L+ EV  LR+ ++ES   + R   +    +   Q +  E+++ ++D   + E+ K L D D  + + +  L  +     G+ S  + LE K         LK D  +  + +ES +        + LK+E+   KT++  ++ +V                 +   G+ S    E+   + K  + TQ+           LS  +     SV+  +  L     +       ++  + +    K+  E      + FK S   + + + ++  TI        ++  DL +LT +L++ +  ID+  S  EH  +++ + K+ +K    L+                      +L  +++   N +DD +K LR  +       EG   K +  E  + +T  E+     ELR+ M   +I   D+E    +   E Q+ T    ++++  E L + +A              +L N+Q K+ EL K    L  +++ +Q+     + ++  + D      EL +N   V+ V EE E+K E+      +++K + + G    K   +++K+E +L ++ KDN                        A+Q+  + +    ++K ++   + EL   R   S+  +  +K  + +++ +  + +   +  E    ++   + ++      L    +E  L    +KD +  + +                  K +D F +   ++I+  N+ ++E  ++   +K     NKV   L    F  KK ++  +    +++   ++    +K D              +  K++++    E++ A I    E+  D+  D+ V   + +    +     + KD+ E L  K+EE  A+     +T +K   A  D   +E +S KK  ++ L      RD+ + D++   ++LE              + HE  + KI ++      DL+L ++ +   E++++ L    D+L ++NE
Sbjct:   52 LEENSEIAQLRSEVAELRQIINESTSGINRVPSSPRPPIRRKQSNTPERTSAALDSKSLDEISKILLDHDVCISDQKKTLARMTKLCLGALSAIAKLENKI--------LKSDFGNHCSDSESNLISIWENTYKQLKKEIIETKTELSSIAEKVSHCP-------------NGNIGIGS----EVSEDIFKLKNQTQI-----------LSDNILRTDKSVIGFQGQLGTLQEQIGNCNEQIKMHLSK----KVGSE------QKFKSSYDNNKEAITKMDLTIG------RLISNDLPNLTIRLKDCESTIDKQVSSAEHDVRIDALHKEFEKKYDSLKS---------------------DLFNELKNCENVYDDQIKVLREGIECVVSKTEGYIDK-NRFENLIENTRKET----VELRENM--GKIIKQDEENTKNIKSLE-QSSTTMAKEIKNCTE-LSLKKA-------------SELENAQKKMDELKKGDDLLLNSVNKLQN---QVNGIVKEIKDCQTKGKELSSNLESVKNVFEE-ERKEEKL-----NISKLETSLG----KTLKSQEKIEKEL-SSNKDNL-----------------------AEQVRSKVEFCSDSLKKEINFCKKELNCCRNEVSDMGKDIIKHLEDEKLHINSSFKPFNKDIETMNHKITSCLGDMNKWNKKLDTFHQEFTLGLNKVKDDSISVKKEVAIDFEASLQENSVCLQKMIDSFMKSTEQDINKHNQKIKEIDNENQIMK-----NKVSEALSGTTFVSKKDLKDIQKNLINDIKSASEQYSGIKADFDDFKLSERSKYTLLDKKQKDINTFCEDINATIYRTEEDIKDIRLDVSVDSNTINELKMKISGINESKDKFEHLSRKVEEYLAQFKSSIQTSSKGVNALEDKYANEFLSVKK-RIDGLVTSKKIRDKLVKDVERIDKKLE--------------QYHEKVMDKIDTNKYSFENDLQLSQEKISLSENSIQKLDKRMDEL-HENE 921          

BLAST of putative restin vs. L. salmonis genes
Match: EMLSAG00000005623 (supercontig:LSalAtl2s:LSalAtl2s301:656013:663945:1 gene:EMLSAG00000005623 transcript:EMLSAT00000005623 description:"maker-LSalAtl2s301-augustus-gene-6.9")

HSP 1 Score: 70.0922 bits (170), Expect = 4.666e-12
Identity = 27/62 (43.55%), Postives = 37/62 (59.68%), Query Frame = 0

Query:   17 GKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGP 78
            G ++F G   FAPG+W G+ LD   GKN+GTV G SYF C   +G+F R + +    +  GP
Sbjct:   23 GVVSFFGFPDFAPGKWVGLTLDTPKGKNNGTVQGRSYFTCPENYGMFVRQTNI----MILGP 80          

HSP 2 Score: 64.6994 bits (156), Expect = 2.326e-10
Identity = 27/70 (38.57%), Postives = 40/70 (57.14%), Query Frame = 0

Query:  121 SMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFA 190
            S A +++G RV + +       G + F G  +FA G W G+ LD+  GKN+G V  + YF CP N+G+F 
Sbjct:    3 SKATVRVGQRVCLKDK--KDVEGVVSFFGFPDFAPGKWVGLTLDTPKGKNNGTVQGRSYFTCPENYGMFV 70          

BLAST of putative restin vs. L. salmonis genes
Match: EMLSAG00000003666 (supercontig:LSalAtl2s:LSalAtl2s19:270880:273523:-1 gene:EMLSAG00000003666 transcript:EMLSAT00000003666 description:"maker-LSalAtl2s19-augustus-gene-2.15")

HSP 1 Score: 61.6178 bits (148), Expect = 1.462e-9
Identity = 24/56 (42.86%), Postives = 33/56 (58.93%), Query Frame = 0

Query:   15 KSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLS 70
            + G++ F+G   F PG W GV  D+  GKNDG+V G  YFKC   +G F +   +S
Sbjct:  670 RRGEVKFIGNVHFKPGIWVGVQYDEPFGKNDGSVEGKRYFKCKDKYGGFVKICFVS 725          

HSP 2 Score: 56.225 bits (134), Expect = 7.545e-8
Identity = 27/71 (38.03%), Postives = 39/71 (54.93%), Query Frame = 0

Query:  123 APLKLGDRV---IVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFA 190
            A +++G+R    + GN     + G ++F+G   F  G W GV+ D   GKNDG V  KRYF+C   +G F 
Sbjct:  651 AKMEVGNRCSITVPGN--ISDRRGEVKFIGNVHFKPGIWVGVQYDEPFGKNDGSVEGKRYFKCKDKYGGFV 719          

BLAST of putative restin vs. SwissProt
Match: gi|261260059|sp|P30622.2|CLIP1_HUMAN (RecName: Full=CAP-Gly domain-containing linker protein 1; AltName: Full=Cytoplasmic linker protein 1; AltName: Full=Cytoplasmic linker protein 170 alpha-2; Short=CLIP-170; AltName: Full=Reed-Sternberg intermediate filament-associated protein; AltName: Full=Restin)

HSP 1 Score: 271.552 bits (693), Expect = 2.345e-72
Identity = 273/869 (31.42%), Postives = 439/869 (50.52%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQ--------PLASGPPSCLTTPRKVSKAGRASMSPS-ESVVDLKSGTPS---PTGS------------TISLVSMAP--------LKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITK------SP---KARMQRQPSSLTPRKLNRERSDLSEASMASMMSTVSRSQ------VPVKKKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSE--------SDAGNSGELIE--KIRSLEDQLAQELKAKADLNGGKSAFETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVD---SLKTRLVATEDKLKEART----HLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLE---REEKNMAALQESLKEQSTKSIDQ-ISELEKALSDK---------------DNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLKQDLKDQLTKAESEIRSLKEELDGAK----TKVEGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQLDKTGSD------TQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVE------EAMQE-LSDVKMAKEDLEKEIRLFKDSSQTSTDE---VKR-LSGTIN---SKDLELEMLRGDLSSLTSKLEEL 769
            +VWV G K G I FLGET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C P+ G+F+R S+L+R+         L + P S  T+P   S A   S SPS  S +  K   P+   P+ +            +IS +S A         LK+GDRV+VG    GTK G +RF+G T+FA+G+W GVELD  +GKNDG V   RYF+C P +GLFAP+HK+TK      +P   KA   R+  + T   L R  S  S +SM+S+ S+VS              +    I     L+ +LKEK+  I+QLL ER+ ERA++AKA S     E  L+   + +  +  E E +    R  +E  + +   L N+LE+EKRK +D++FR+EEE++ + +         D  N+   +E  +I+ LE  L  E K KAD    +   +TR+A +     SE+    +  +D +    E+  L  +LE+    N+  G +VD   SL   + + ++KL+  RT     +  +KE  G R    +++I  L    + L K+ +  K  LE   +E  ++ AL +S  E +  S  Q + EL+ + S                 + + L+ QH++++LQ+   S      E++   KE   L+  L   + + E+ + +++ +LD A+     ++E   N++ + +  +K LE  + K ++Q    +K ID   SQL  T          + A  E   ++ +L  +LE A+  +  L+ +   E +KA+    +++        +QE LS+V   KE LEKE+++ K+    +++E   V+R +  T+N    K+ +  ML  DL  L   L ++
Sbjct:   63 RVWVNGNKPGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLKGIFTRPSKLTRKVQAEDEANGLQTTPASRATSPLCTSTASMVSSSPSTPSNIPQKPSQPAAKEPSATPPISNLTKTASESISNLSEAGSIKKGERELKIGDRVLVG----GTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTKIGFPSTTPAKAKANAVRRVMATTSASLKRSPSASSLSSMSSVASSVSSRPSRTGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHVGEIEQELALARDGHDQHVLELEAKMDQLRTMVEAADREKVELLNQLEEEKRKVEDLQFRVEEESITKGDLEQKSQISEDPENTQTKLEHARIKELEQSLLFE-KTKAD-KLQRELEDTRVATV-----SEKSRIMELEKDLALRVQEVAELRRRLES----NKPAG-DVDMSLSLLQEISSLQEKLEVTRTDHQREITSLKEHFGAREETHQKEIKALYTATEKLSKENESLKSKLEHANKENSDVIALWKSKLETAIASHQQAMEELKVSFSKGLGTETAEFAELKTQIEKMRLDYQHEIENLQNQQDS------ERAAHAKEMEALRAKLMKVIKEKENSLEAIRSKLDKAEDQHLVEMEDTLNKLQEAEIKVKELEVLQAKCNEQ----TKVIDNFTSQLKATEEKLLDLDALRKASSEGKSEMKKLRQQLEAAEKQIKHLEIEKNAESSKASSITRELQGRELKLTNLQENLSEVSQVKETLEKELQILKEKFAEASEEAVSVQRSMQETVNKLHQKEEQFNMLSSDLEKLRENLADM 905          

HSP 2 Score: 154.836 bits (390), Expect = 2.495e-36
Identity = 88/221 (39.82%), Postives = 126/221 (57.01%), Query Frame = 0

Query: 1545 FAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRSGRK---SPSPAKSISSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVA--PRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            F +DA E E+  L + +  +  LL +K  E +  + E   L  EN    S    V  LE +K +LE +V  L  + +  ++   S S      +D ++   ++Q+DFLNSVIVD+QRKN DLK K+E+   A +  +  + N   +    +     PRLFCDICD FD+HDTEDCPTQA ++SE   H+   G+RG+ R YC+ CE+FGH   NCN+ ETF
Sbjct: 1220 FIKDADE-EKASLQKSISITSALLTEKDAELEKLRNEVTVLRGENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQA-QMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDETF 1438          

BLAST of putative restin vs. SwissProt
Match: gi|81879884|sp|Q922J3.1|CLIP1_MOUSE (RecName: Full=CAP-Gly domain-containing linker protein 1; AltName: Full=Cytoplasmic linker protein 170; Short=CLIP-170; AltName: Full=Restin)

HSP 1 Score: 268.47 bits (685), Expect = 1.804e-71
Identity = 256/847 (30.22%), Postives = 410/847 (48.41%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLA-------SGPPSCLTTPRKVSKAGRASMS----------PSESVVDLKSGTPSPTGST------ISLVSMAP--------LKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITK------SPKARMQRQ--------PSSLTPRKLNRERSDLSEASMASMMSTVSRSQVPVKKKSIAS--IMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGEL-----IEKIRSLEDQLAQELKAKADLNGGKSAFETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRLVATEDKLKE---ARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLKQDLKDQLTKAESEIRSLKEELDGAK----TKVEGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQLDKTGS---DTQVALQEKSEKVIELSS---KLEDAQSSVVALKTDLELEHAKAARAKADVEE------AMQE-LSDVKMAKEDLEKEIRLFKDSSQTSTDEV----KRLSGTINS----------KDLELEMLRGDLSSLTSKLEE 768
            +VWV G K G I FLGET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C P+ G+F+R S+L+R+  A          P    +P   + A   S S          PS+S     S TP  +  T      IS +S A         LK+GDRV+VG    GTK G +RF+G T+FA+G+W GVELD  +GKNDG V   RYF+C P +GLFAP+HK+TK      +P               P+SL  R  +        +  +S+ S  SR+ +  +  S  +  I     L+ +LKEK+  I+QLL ER+ ERA++AKA S     E  L+   + +  +  E E +    R  +E  + +   L N+LE+EKRK +D++FR+EEE++ + + +     E      +EK  +L  Q   EL+ + + +      +  ++ L+     +EK+    T  Q     EM +L E       + EA  KE+ +L T   ATE   KE    R+ LD   +E+ +        I + K +++T          ++   ++ M  L+ S  +       + +EL+  +   + L L+ QH+++SLQS   S      E+S   KE   ++  L   + + E  + ++K  LD A+     ++E   N++ + +  +K LE  + K  +Q    S+ I    SQL        D     +  SE  +EL +   +LE A+  +  L+T+   E +KA     +++E       +Q+ L+ V   KE LEKE++  K+   ++++E      R+  T+N              ELE LR +L+ + +K +E
Sbjct:   63 RVWVNGNKPGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLKGIFTRPSKLTRKVQAEDEANGLQAAPGRTASPLSTAAATMVSSSPATPSNIPHKPSQSTAKEPSATPQISNLTKTASESISNLSEAGSVKKGERELKVGDRVLVG----GTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTKIGFPSTTPAKAKAAAVRRVMAATPASLK-RSPSASSLSSMSSVASSVSSKPSRTGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHVGEIEQELALARDGHDQHVLELEAKMDQLRTMVEAADREKVELLNQLEEEKRKVEDLQFRVEEESITKGDLEVATVSEKSRIMELEKDLALRAQEVAELRRRLESSKPPGDVDMSLSLLQEISALQEKLEAIHTDHQG----EMTSLKEHFGA---REEAFQKEIKALHT---ATEKLSKENESLRSKLDHANKENSDV-------IALWKSKLET----------AIASHQQAMEELKVSFSKGIGTDSAEFAELKTQI---ERLRLDYQHEIESLQSKQDS------ERSAHAKEMETMQAKLMKIIKEKEDSLEAVKARLDSAEDQHLVEMEDTLNKLQEAEIKVKELEVLQAKYTEQ----SEVIGNFTSQLSAVKEKLLDLDALRKANSEGKLELETLRQQLEGAEKQIKNLETERNAESSKANSITKELQEKELVLTGLQDSLNQVNQVKETLEKELQTLKEKFASTSEEAVSAQTRMQDTVNKLHQKEEQFNVLSSELEKLRENLTDMEAKFKE 864          

HSP 2 Score: 150.214 bits (378), Expect = 6.218e-35
Identity = 99/284 (34.86%), Postives = 151/284 (53.17%), Query Frame = 0

Query: 1500 EAETERDSARSKSKDFQKISTQNVTLDED----------NENLKKDIEELKHKL--------HFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRSGRK---SPSPAKSISSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVA--PRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            E ET + +A  KS+    +  +NV L E+          ++ L+++   L ++L         F +DA E E+  L + +  +  LL +K  E +  + E   L  EN    S    V  LE +K +LE +V  L  + +  ++   S S      ++ ++   ++Q+DFLNSVIVD+QRKN DLK K+E+   A +  +  + N   +    +     PRLFCDICD FD+HDTEDCPTQA ++SE   H+   G+R + R YC+ CE+FGH   NCN+ ETF
Sbjct: 1110 EIETLKQAAAQKSQQLSALQEENVKLAEELGRTRDEVTSHQKLEEERSVLNNQLLEMKKRESEFRKDADE-EKASLQKSISLTSALLTEKDAELEKLRNEVTVLRGENATAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDAQAEEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGEDLNSYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQA-QMSEDPPHSTHHGSRSEERPYCEICEMFGHWATNCNDDETF 1391          

BLAST of putative restin vs. SwissProt
Match: gi|74753268|sp|Q9UDT6.1|CLIP2_HUMAN (RecName: Full=CAP-Gly domain-containing linker protein 2; AltName: Full=Cytoplasmic linker protein 115; Short=CLIP-115; AltName: Full=Cytoplasmic linker protein 2; AltName: Full=Williams-Beuren syndrome chromosomal region 3 protein; AltName: Full=Williams-Beuren syndrome chromosomal region 4 protein)

HSP 1 Score: 261.922 bits (668), Expect = 2.229e-70
Identity = 151/380 (39.74%), Postives = 219/380 (57.63%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPPS------CLTTPRKVSKAGRASMSPSESVVDLKSG-------TPSPTGSTISLVSMAP-----LKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITK------SP-KA----RMQRQPSSLTPRKLNRERSDLSEASMASMMSTVSRSQVPVKKKSIAS--IMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSE 356
            +VWV G K G + +LGET+FAPG+WAGV LDD +GKNDG VGGV YF+C  + G+F+R S+L+RQP A G  S       LT       +G A+   +  V+ L+         T + +GS +S           L+LGDRV+VG    GTK G +R++G T+FA+G+W GVELD  +GKNDG V   RYF+CPP FGLFAP+HK+ +      SP KA    RM    S+LT    +        +  +S+    SRS +  +  S  +  I     L+ +LKEK+  I+QLL ER+ ERA++AKA S     E  ++ +  ++  Y  E EE+    R  +E   ++   L N+LE+E+RK +D++FR+EEE++ + +
Sbjct:   84 RVWVNGVKPGVVQYLGETQFAPGQWAGVVLDDPVGKNDGAVGGVRYFECPALQGIFTRPSKLTRQPTAEGSGSDAHSVESLTAQNLSLHSGTATPPLTSRVIPLRESVLNSSVKTGNESGSNLSDSGSVKRGEKDLRLGDRVLVG----GTKTGVVRYVGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCPPKFGLFAPIHKVIRIGFPSTSPAKAKKTKRMAMGVSALT-HSPSSSSISSVSSVASSVGGRPSRSGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHICEVEKEIALLKAQHEQYVAEAEEKLQRARLLVESVRKEKVDLSNQLEEERRKVEDLQFRVEEESITKGD 458          

BLAST of putative restin vs. SwissProt
Match: gi|76364161|sp|Q9Z0H8.2|CLIP2_MOUSE (RecName: Full=CAP-Gly domain-containing linker protein 2; AltName: Full=Cytoplasmic linker protein 115; Short=CLIP-115; AltName: Full=Cytoplasmic linker protein 2)

HSP 1 Score: 258.07 bits (658), Expect = 4.076e-69
Identity = 151/380 (39.74%), Postives = 218/380 (57.37%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPPS------CLTTPRKVSKAGRASMSPSESVVDLKSG-------TPSPTGSTISLVSMAP-----LKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITK------SP-KA----RMQRQPSSLTPRKLNRERSDLSEASMASMMSTVSRSQVPVKKKSIAS--IMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSE 356
            +VWV G K G + +LGET+FAPG+WAGV LDD +GKNDG VGGV YF+C  + G+F+R S+L+RQP A G  S       LT       +G A+   +  V+ L+         T + +GS +S           L LGDRV+VG    GTK G +R++G T+FA+G+W GVELD  +GKNDG V   RYF+CPP FGLFAP+HK+ +      SP KA    RM    S+LT    +        +  +S+    SRS +  +  S  +  I     L+ +LKEK+  I+QLL ER+ ERA++AKA S     E  ++ +  ++  Y  E EE+    R  +E   ++   L N+LE+E+RK +D++FR+EEE++ + +
Sbjct:   85 RVWVNGVKPGVVQYLGETQFAPGQWAGVVLDDPVGKNDGAVGGVRYFECPALQGIFTRPSKLTRQPTAEGSGSDTHSVESLTAQNLSLHSGTATPPLTGRVIPLRESVLNSSVKTGNESGSNLSDSGSVKRGDKDLHLGDRVLVG----GTKTGVVRYVGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCPPKFGLFAPIHKVIRIGFPSTSPAKAKKTKRMAMGVSALT-HSPSSSSISSVSSVASSVGGRPSRSGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHICEVEKEIALLKAQHEQYVAEAEEKLQRARLLVENVRKEKVDLSNQLEEERRKVEDLQFRVEEESITKGD 459          

BLAST of putative restin vs. SwissProt
Match: gi|1109546125|sp|Q9JK25.2|CLIP1_RAT (RecName: Full=CAP-Gly domain-containing linker protein 1; AltName: Full=Cytoplasmic linker protein 170; Short=CLIP-170; AltName: Full=Restin)

HSP 1 Score: 258.84 bits (660), Expect = 1.472e-68
Identity = 243/782 (31.07%), Postives = 384/782 (49.10%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPPSCLTT-------PRKVSKAGRASMS----------PSESVVDLKSGTPSPTGST------ISLVSMAP--------LKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITK------SP--------KARMQRQPSSLTPRKLNRERSDLSEASMASMMSTVSRSQVPVKKKSIAS--IMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKAD-----LNGGKSAF---ETRIADLEGKLG------SEEKMRTDWTRDQSQMKI------EMDNLAEQLETEHRKNEARGKEVDSLKTRLVATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQ-ISELEKALSDK---------------DNLLLESQHKLDSLQSGSSSVQST----LEEKSRELKENLKLKQD----LKDQLTKAES----EIRSLKEELDGAKTKVEGLSNQVGQRDETIKSLEGQKTKIHK---QFGVKSKEIDELKSQLDKTGSDTQVALQEKSEK---VIELSSKLEDAQSSVVALKTD 688
            +VWV G K G I FLGET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C P+ G+F+R S+L+R+  A    + L T       P   + A   S S          PS+ V    S TP  +  T      IS +S A         LK+GDRV+VG    GTK G +RF+G T+FA+G+W GVELD  +GKNDG V   RYF+C P +GLFAP+HK+TK      +P        +  M   P+SL  R  +        +  +S+ S  SR+ +  +  S  +  I     L+ +LKEK+  I+QLL ER+ ERA++AKA S     E  L+   + +  +  E E +    R  +E  + +   L N+LE+EKRK +D++FR+EEE++ + + +     E   +I+ LE  L  E K KAD     L   + A    ++RI +LE  L       +E + R + ++    + +      E+  L E+LE  H  ++    EV SLK      E+  ++    L    E+              L  E ++LR  LD +     +E  ++ AL +S  E +  S  Q + EL+ + S                 + L L+ QH+++SLQS   S +S     +E    +L + +K K+D    +K +L  AE     E+  +  +L  A+ K E  ++   +   T  S++    K+H+   QF + S E+++L+  L    +D +   +EK E+   +++   KLE+  + ++ +  D
Sbjct:   63 RVWVNGNKPGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLKGIFTRPSKLTRKVQAEDEANGLQTAHARAASPLSTAAATMVSSSPATPSNIPQKPSQPVAKETSATPQISNLTKTASESISNLSEAGSVKKGERELKIGDRVLVG----GTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTKIGFPSTTPAKAKAAAVRRVMATTPASLK-RSPSASSLSSMSSVASSVSSKPSRTGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHVGEIEQELALARDGHDQHVLELEAKMDQLRTMVEAADREKVELLNQLEEEKRKVEDLQFRVEEESITKGDLETQTKLEHA-RIKELEQSLLFE-KTKADKLQRELEDTRVATVSEKSRIMELEKDLALRVQEVAELRRRLESSKPPGDVDMSLSLLQEISALQEKLEVTHTDHQ---NEVTSLKDHFGTREEMFQKEIKALHAATEK--------------LSKENESLRSKLDHAN----KENSDVIALWKSKLETAIASHQQAMEELKVSFSKGIGTDSAEFAELKTQIERLRLDYQHEIESLQSKQDSERSAHAKEMESMKAKLMKIIKEKEDSLEAVKARLDTAEDQHLVEMEEMLSKLQEAEIKKEKFASASEEAVSTQTSMQDTVNKLHQKEEQFNMLSSELEKLRENL----TDMEAKFKEKDEREDQLVKAKEKLENDIAEIMKMSGD 812          

HSP 2 Score: 147.902 bits (372), Expect = 3.036e-34
Identity = 85/223 (38.12%), Postives = 124/223 (55.61%), Query Frame = 0

Query: 1545 FAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRSGRKSPSPAKSISSDMNDGELKAQ-----VDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVA--PRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            + +DA E E+  L + +  +  LL +K  E +  + E   L  EN    S    V  LE +K +LE +V  L  + +  ++  S +   +    + + +AQ     +DFLNSVIVD+QRKN DLK K+E+   A +  +  + N   +    +     PRLFCDICD FD+HDTEDCPTQA ++SE   H+   G+R + R YC+ CE+FGH   NCN+ ETF
Sbjct: 1100 YRKDADE-EKASLQKSISLTSALLTEKDAELEKLRNEVTVLRGENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDVQTEEDERAQESQQMIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGEDPNSYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQA-QMSEDPPHSTHHGSRSEERPYCEICEMFGHWATNCNDDETF 1320          

BLAST of putative restin vs. SwissProt
Match: gi|76364162|sp|O55156.1|CLIP2_RAT (RecName: Full=CAP-Gly domain-containing linker protein 2; AltName: Full=Cytoplasmic linker protein 115; Short=CLIP-115; AltName: Full=Cytoplasmic linker protein 2)

HSP 1 Score: 256.144 bits (653), Expect = 1.638e-68
Identity = 150/380 (39.47%), Postives = 218/380 (57.37%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPPS------CLTTPRKVSKAGRASMSPSESVVDLKSG-------TPSPTGSTISLVSMAP-----LKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITK------SP-KA----RMQRQPSSLTPRKLNRERSDLSEASMASMMSTVSRSQVPVKKKSIAS--IMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSE 356
            +VWV G K G + +LGET+FAPG+WAGV LDD +GKNDG VGG+ YF+C  + G+F+R S+L+RQP A G  S       LT       +G A+   +  V+ L+         T + +GS +S           L LGDRV+VG    GTK G +R++G T+FA+G+W GVELD  +GKNDG V   RYF+CPP FGLFAP+HK+ +      SP KA    RM    S+LT    +        +  +S+    SRS +  +  S  +  I     L+ +LKEK+  I+QLL ER+ ERA++AKA S     E  ++ +  ++  Y  E EE+    R  +E   ++   L N+LE+E+RK +D++FR+EEE++ + +
Sbjct:   85 RVWVNGVKPGVVQYLGETQFAPGQWAGVVLDDPVGKNDGAVGGLRYFECPALQGIFTRPSKLTRQPAAEGSGSDGHSVESLTAQNLSLHSGTATPPLTGRVIPLRESVLNSSVKTGNESGSNLSDSGSVKRGDKDLHLGDRVLVG----GTKTGVVRYVGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCPPKFGLFAPIHKVIRIGFPSTSPAKAKKTKRMAMGVSALT-HSPSSSSISSVSSVASSVGGRPSRSGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHICEVEKEIALLKAQHEQYVAEAEEKLQRARLLVENVRKEKVDLSNQLEEERRKVEDLQFRVEEESITKGD 459          

BLAST of putative restin vs. SwissProt
Match: gi|3024541|sp|O42184.1|CLIP1_CHICK (RecName: Full=CAP-Gly domain-containing linker protein 1; AltName: Full=Cytoplasmic linker protein 170; Short=CLIP-170; AltName: Full=Restin)

HSP 1 Score: 257.299 bits (656), Expect = 5.932e-68
Identity = 268/876 (30.59%), Postives = 434/876 (49.54%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPL----ASGP----------------------------PSCLTTPRKVSK-AGRASMSPSE--SVVDLKSGTPSPTGSTISL-VSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITK------SP-KARMQRQPSSLTPRKLNR----ERSDLSEASMASMMSTVSRSQVPVKKKSIAS--IMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKADLNGGKSAFETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARG-----KEVDSLKTRLVATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQI----SELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELK-------ENLKLKQDLKDQLTKAESEIRSLK-----------EELDGAKTKVEGLSNQ-VGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQLDK----TGSDTQ------------VALQE-KSEKVIE---LSSKLEDAQSSVVALKTDLELEHAKAARAK----ADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEV----KRLSGTINSKDL----------ELEMLRGDLSSLTSKLEE 768
            +VWV G K G I FLGET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C P+ G+F+R S+LSR+ L    A+G                             PS +  P+K S  A +   +PS+  ++    SG+ S      SL      LK+GDRV+VG    GTK G +RF+G T+FA+G+W GVELD  +GKNDG V   RYF+C P +GLFAP+HK+TK      +P KA+   +    TP  L R           +  +S+ S  SR+ +  +  S  +  I     L+ +LKEK+  I+QLL ER+ ERA++AKA S     E  L+ + + +  +  E E +    RA +E  + +   L N+LE+EKRK +D++FR+EEE++ + + +     E   +I+ LE  L  E K KAD    +   +TR+A +     SE+    +  RD +    E+  L  +LE+    ++        +E+ SL+ ++ A     KE +  +  +KE+     FES E         + LRK++     S ER  K   +L+  L   + ++ D I    S+LE A++     + E +   +      ++  + L+ +  ++K        NLKLKQ+  ++ ++   EI +LK           + L+  K K+E + +Q + + ++T+  L+  + K+        KE+D L+++ ++     GS TQ             ALQ+  SE  +E   LS +L+ A+  +  L+T+      K  + K     D+E   + LS V   K+ LEKE++L K+   ++ D      + +  TIN  +           ELE L+ +L+ + +KL+E
Sbjct:   64 RVWVNGNKPGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLRGIFTRPSKLSRKVLTEDEANGTQTAHASRATSPTSTSTASAVSASPAALLPSGI--PQKTSPLAAKEHSTPSQFSNLSKTASGSVSNLSEAGSLKKGERELKIGDRVLVG----GTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPRYGLFAPVHKVTKIGFPSTTPAKAKTTVRKVVATPAALKRSPSASSLSSLSSVASSVSSKPSRTGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHVGEIEQELALVRDGHDRHVLEMEAKMDQLRAMVEAADREKVELLNQLEEEKRKVEDLQFRVEEESITKGDLETQTKLEHA-RIKELEQSLLFE-KTKAD-KLQRELEDTRVATV-----SEKSRIMELERDLALRVKEVAELRGRLESSKHIDDVDTSLSLLQEISSLQEKMAAAG---KEHQREMSSLKEK-----FESSE---------EALRKEIKTLSASNERMGKENESLKTKLDHANKENSDVIELWKSKLESAIASHQQAMEELKVSFNKGVGAQTAEFAELKTQMEKVKLDYENEMSNLKLKQE--NEKSQHLKEIEALKAKLLEVTEEKEQTLENLKAKLESVEDQHLVEMEDTLNKLQEAEIKV--------KELDVLQAKCNEQTKLIGSLTQQIRASEEKLLDLAALQKANSEGKLEIQKLSEQLQAAEKQIQNLETEKVSNLTKELQGKEQKLLDLE---KNLSAVNQVKDSLEKELQLLKEKFTSAVDGAENAQRAMQETINKLNQKEEQFALMSSELEQLKSNLTVMETKLKE 895          

HSP 2 Score: 160.614 bits (405), Expect = 3.551e-38
Identity = 235/914 (25.71%), Postives = 434/914 (47.48%), Query Frame = 0

Query:  961 EEAIHAMQSTSVDSDALIGNLNDELK-----ANKAE---VERVREELEQKVEQHQKDLDDLA----KEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQFDDEL--TRAKL-----QADQILEEQKSRERAMKNQ----MEDLQGELKMRGSTSEETEKALKDEKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEIDLADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGG-NKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDIT---NKEEELEFAKEELE-------------------------AKIEEFNDLNTDLEVKEASFDNS---FKETDE-----KHKDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILDLKASLEELEGRVTSLNSSSADEGKIHEAKVAKITSDNEDLRLKKKDL---ESTLKSLQADFDQLSNQNEDL------------------SIKVVERE----HQVR-EYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVDELQIALAEAETERDSARSKSKDFQKISTQNVTL-----------------DEDNENLKKDIEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRSGRKSP----SPAKSISSDM---NDGELKAQ-VDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEA-NFMFN--GVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            EEA+     T   S+  +G  N+ LK     ANK     +E  + +LE  +  HQ+ +++L     K   A+ A+  ++ +  +K++LD E    +  + +EN    K+Q   E+   +AKL     + +Q LE  K++  ++++Q    MED   +L+      +E +  L+ + +++ +L  ++ +  R+ EE++ +L  +       ++++   +E+L+ AEK I++L                  ++++  L K L+  + +++ L++++   N+V+     D  +K+++  K KF   +D   +  ++M++ I     KEE+      ELE                         AK++  ND+    E+ ++S D+S    K  DE     +  ++I++ +TK   A  +   L + + +T  + +    E +   + E+  +QDQ++       D+K  +E      TS N     + K  +     IT  + D++  K++L   E  LK+ Q   D+L  Q E+L                  +++ V +E    HQ + E  + ++  + + EK Q EL    + + ++ + L + ++ L+ E  +V+EL+    E E  + +A  KS+    +  +NV L                 +E+   L   + E+K +    +   + ER  L + +  +  L+  K EE +  + E   L  EN    + +  V  LE +K +LE +V  L  + ++  + P    SP+  I++++      E K Q +DFLNSVIVD+QR+N++L  K++    A +  +E E  N+     G+S ++  PRLFCDIC  FD+HDTEDCPTQA  + EP  H+   G+R + R YCDTCE+FGH TA+CN+ ETF
Sbjct:  571 EEALRKEIKTLSASNERMGKENESLKTKLDHANKENSDVIELWKSKLESAIASHQQAMEELKVSFNKGVGAQTAEFAELKTQMEKVKLDYENEMSNLKLKQENE---KSQHLKEIEALKAKLLEVTEEKEQTLENLKAKLESVEDQHLVEMEDTLNKLQEAEIKVKELD-VLQAKCNEQTKLIGSLTQQIRASEEKLLDLAALQKANSEGKLEIQKLSEQLQAAEKQIQNL-----------------ETEKVSNLTKELQGKEQKLLDLEKNLSAVNQVK-----DSLEKELQLLKEKFTSAVDGAENAQRAMQETINKLNQKEEQFALMSSELEQLKSNLTVMETKLKEREEREQQLTEAKVKLENDI---AEIMKSSGDSSAQLMKMNDELRLKERQLEQIQLELTK---ANEKAVQLQKNVEQTAQKAEQSQQETLKTHQEELKKMQDQLT-------DMKKQME------TSQNQYKDLQAKYEKETSEMITKHDADIKGFKQNLLDAEEALKAAQKKNDELETQAEELKKQAEQAKADKRAEEVLQTMEKVTKEKDAIHQEKIETLASLENSRQTNEKLQNELDMLKQNNLKNEEELTKSKELLNLENKKVEELK---KEFEALKLAAAQKSQQLAALQEENVKLAEELGRSRDEVTSHQKLEEERSVLNNQLLEMKKRESTLKKEIDEERASLQKSISDTSALITQKDEELEKLRNEITVLRGENASAKTLQSVVKTLESDKLKLEEKVKNLEQKLKAKSEQPLTVTSPSGDIAANLLQDESAEDKQQEIDFLNSVIVDLQRRNEELNLKIQRMCEAALNGNEEETINYDSEEEGLSKKT--PRLFCDICGCFDLHDTEDCPTQAQMLEEPP-HSTYHGSRREERPYCDTCEMFGHWTADCNDDETF 1433          

BLAST of putative restin vs. SwissProt
Match: gi|30580382|sp|Q9VJE5.1|CL190_DROME (RecName: Full=Restin homolog; AltName: Full=Cytoplasmic linker protein 190; AltName: Full=Microtubule-binding protein 190; AltName: Full=d-CLIP-190)

HSP 1 Score: 227.254 bits (578), Expect = 2.063e-58
Identity = 155/481 (32.22%), Postives = 243/481 (50.52%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASG--PPSCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISLVSMAP-------LKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSP---KARMQRQPSSLTPRKLNRERSDLSEASMASMMSTVSR-SQVPVKKKSIASIMAPERLKMS----LKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKA--------KADLNGGKSAFETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNE---ARGKEVDSLKTRLVATEDKL 460
            +VW+GGT+ G+IAF+G+T FA GEWAGV LD+  GKNDG V G  YF+C P  G+FSR +RL+  PLA    P S L           A  SP       +S T SPT S  S +  +P       + +GDRVIV +   G++ G LR++G T+FA G+W GVELD   GKNDG V D RYFEC P +G+F P+ K++ SP   K R+ R  S  +   +    S  + A+    M+   R S  PVK      I+A  + + S    L+EK+  +++L++ER+ +R                             + + +AL  +  + + + +I  L++ L++E++K ++++  ++E      E +A  S    EKI  LE ++ + + A          DL     A +  IA L+ K+  ++K            ++E   +AEQLE E R  E      +++ +L++ LV+ ++ L
Sbjct:  128 RVWLGGTRPGQIAFIGDTHFAAGEWAGVVLDEPNGKNDGCVSGKRYFQCEPKRGIFSRLTRLTTYPLAGAQTPTSPL-----------AKSSPD------RSRTVSPTASIRSSMLRSPGIGGKNGMAVGDRVIVSSGF-GSRPGILRYLGETQFAPGNWCGVELDEPSGKNDGTVDDIRYFECKPKYGVFVPIAKVSLSPSSKKTRLSRTGSRESLTSIGTMNSIATTATSRMRMNAQQRKSSTPVK-----PILATPKSQFSMQDLLREKQQHVEKLMVERDLDR----------------------------EDAQNQALQLQKNINELKARIVELESALDNERKKTEELQCSIDEAQFCGDELNA-QSQVYKEKIHDLESKITKLVSATPSLQSILPPDLPSDDGALQEEIAKLQEKMTIQQK------------EVE-SRIAEQLEEEQRLRENVKYLNEQIATLQSELVSKDEAL 543          

HSP 2 Score: 134.806 bits (338), Expect = 3.168e-30
Identity = 104/315 (33.02%), Postives = 169/315 (53.65%), Query Frame = 0

Query: 1472 QDGDALKELEKALDDERIRVDELQIALAEAETERDSARSKSKDFQ----KISTQNVTLDEDNENLKKDIEELKHKLHFAQDAFESERTELNEVVQ--------KSKVL--LQDKVEEFKSQKREFD----ALVLENQQLNSYRRKVMILEQEK-RELESQVVTLT-YESRSGRKSPSPAKSISSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAME---ISEPVIHTKSGGARGQI---REYCDTCEVFGHSTANCNEGETF 1760
            Q  + LKEL+  LD+    ++  + +  E + + + A+ K +  Q    K++ Q   L + NE L+K +++ K  L    + F+++  E  +V+         KS +L  LQ++V E ++  R+ +       LE ++L   RR++  LE EK RE+ S    +    SRSG+     +  I + +      A+++FLNS+I DMQ+KND LK K++  E+  +  ++  A   F+ ++ R  APRLFCDICD FD HDTEDCP Q  E    S P   + +     ++   R+YCD+CEVFGH T+ C + ET+
Sbjct: 1389 QKENGLKELQGKLDESNTVLESQKKSHNEIQDKLEQAQQKERTLQEETSKLAEQLSQLKQANEELQKSLQQ-KQLLLEKGNEFDTQLAEYQKVIDEMDDAASVKSALLEQLQNRVAELETALRQANDAQKTAYLETKEL---RRQLESLELEKSREVLSLKAQMNGASSRSGKGDEVESLDIETSL------AKINFLNSIIADMQQKNDALKAKVQTLETLPMDFTKPHA---FDALTKRKPAPRLFCDICDEFDQHDTEDCPIQGSEDQDYSTPSSESNNNEKERKLPAPRKYCDSCEVFGHDTSECADDETY 1690          

BLAST of putative restin vs. SwissProt
Match: gi|75061679|sp|Q5R686.1|CLIP3_PONAB (RecName: Full=CAP-Gly domain-containing linker protein 3; AltName: Full=Cytoplasmic linker protein 170-related 59 kDa protein; Short=CLIP-170-related 59 kDa protein; Short=CLIPR-59)

HSP 1 Score: 150.599 bits (379), Expect = 1.518e-36
Identity = 83/199 (41.71%), Postives = 113/199 (56.78%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPPSCLT-TPR-------KVSKAGRASMSPSESVVDLKSGTP-SPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITK 197
            +V + G K+G + F G T+FA G+W GV LD+  GKNDG+VGGV YF C P  G+F+  S++S+      PPS +T TPR       +V+  GR            K  TP SP+  ++     A  ++GD+V+V     G K G +RF G T+FA G W G+ELD   GK+DG V   RYF CPP  G+FAP  +I +
Sbjct:  299 RVLLDGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKISKA--VDAPPSSVTSTPRTPRMDFSRVTGKGRREHKG-------KKKTPSSPSLGSLQQRDRAKAEVGDQVLVA----GQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQR 484          

HSP 2 Score: 100.908 bits (250), Expect = 2.244e-20
Identity = 69/176 (39.20%), Postives = 92/176 (52.27%), Query Frame = 0

Query:  119 LVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSL--TPRKLNRERSDLSEASMASMMSTVSRSQVPVKKKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEG 292
            ++S   L+LGDRV++     G K GTLRF G TEFA G W GVELD   GKNDG VG  RYF CPP  GLFA + KI+K+  A     PSS+  TPR     R D S  +         + + P    S+ S+   +R K  +       DQ+L+  +++   I +   +TD A G
Sbjct:  288 MLSALGLRLGDRVLL----DGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKISKAVDA----PPSSVTSTPRT---PRMDFSRVTGKGRREHKGKKKTP-SSPSLGSLQQRDRAKAEVG------DQVLVAGQKQ--GIVRFYGKTDFAPG 443          

HSP 3 Score: 80.8777 bits (198), Expect = 4.280e-14
Identity = 38/83 (45.78%), Postives = 50/83 (60.24%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPPSCLTTPRKVSK 90
            +V V G K G + F G+T FAPG W G+ LD   GK+DG+V GV YF C P HGVF+ ASR+ R   ++  P      +KV +
Sbjct:  421 QVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQRIGGSTDSPGDSVGAKKVHQ 503          

BLAST of putative restin vs. SwissProt
Match: gi|116241302|sp|Q96DZ5.3|CLIP3_HUMAN (RecName: Full=CAP-Gly domain-containing linker protein 3; AltName: Full=Cytoplasmic linker protein 170-related 59 kDa protein; Short=CLIP-170-related 59 kDa protein; Short=CLIPR-59)

HSP 1 Score: 150.214 bits (378), Expect = 1.963e-36
Identity = 83/199 (41.71%), Postives = 113/199 (56.78%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPPSCLT-TPR-------KVSKAGRASMSPSESVVDLKSGTP-SPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITK 197
            +V + G K+G + F G T+FA G+W GV LD+  GKNDG+VGGV YF C P  G+F+  S++S+      PPS +T TPR       +V+  GR            K  TP SP+  ++     A  ++GD+V+V     G K G +RF G T+FA G W G+ELD   GK+DG V   RYF CPP  G+FAP  +I +
Sbjct:  299 RVLLDGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKISKA--VDAPPSSVTSTPRTPRMDFSRVTGKGRREHKG-------KKKTPSSPSLGSLQQRDGAKAEVGDQVLVA----GQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQR 484          

HSP 2 Score: 97.4413 bits (241), Expect = 2.272e-19
Identity = 68/176 (38.64%), Postives = 91/176 (51.70%), Query Frame = 0

Query:  119 LVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSL--TPRKLNRERSDLSEASMASMMSTVSRSQVPVKKKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEG 292
            ++S   L+LGDRV++     G K GTLRF G TEFA G W GVELD   GKNDG VG  RYF CPP  GLFA + KI+K+  A     PSS+  TPR     R D S  +         + + P    S+ S+   +  K  +       DQ+L+  +++   I +   +TD A G
Sbjct:  288 MLSALGLRLGDRVLL----DGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKISKAVDA----PPSSVTSTPRT---PRMDFSRVTGKGRREHKGKKKTP-SSPSLGSLQQRDGAKAEVG------DQVLVAGQKQ--GIVRFYGKTDFAPG 443          

HSP 3 Score: 80.8777 bits (198), Expect = 4.243e-14
Identity = 38/83 (45.78%), Postives = 50/83 (60.24%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPPSCLTTPRKVSK 90
            +V V G K G + F G+T FAPG W G+ LD   GK+DG+V GV YF C P HGVF+ ASR+ R   ++  P      +KV +
Sbjct:  421 QVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQRIGGSTDSPGDSVGAKKVHQ 503          

BLAST of putative restin vs. nr
Match: gi|1325320946|ref|XP_023342274.1| (CAP-Gly domain-containing linker protein 1-like [Eurytemora affinis])

HSP 1 Score: 656.751 bits (1693), Expect = 0.000e+0
Identity = 568/1671 (33.99%), Postives = 923/1671 (55.24%), Query Frame = 0

Query:  137 VTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSLTPRKLNRERSDLSEASMASMM-----STVSRSQ-----VPVKKKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELI--------EKIRSLEDQLAQELKAKADLNGGKSAFETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRLVATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLKQDLKDQLTKAESEIRSLKEELDGAKTKVEGLSNQVGQRDETIKSLEGQKTKIHKQ-----FGVKSKEIDELKSQLDKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQDQLEQIQKDAKKTTHELEEKLKKSEATKEKAEKAKEEAIGELHGKIRQTANDHDDLVKSLR----GDLSKKTYDLEGLQSKFSE--LEKSLL-----STSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATIS----DLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNL---NDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVK------------ISSAKDKLELDLETTQKDNAVLKENSILAKAQFDDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKDE--KSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEIDLADKTEELELAEKSIKDLNKKL----GQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFNDLNTDLEVKEASFDNSFKETDEKHKDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILDLKASLEELEGRVTSLNSSSADEGKIHEAKVAKITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVDELQIALAEAETERDSARSKSKDFQKISTQN---VTLDEDNENLKKDIEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRSGR-KSPSPAKSISSDMNDGE--LKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGAR-GQIREYC 1741
            + GTK GTL+++GPT+FA+G+W G+EL++ IGKNDG V  K+YFEC  NFGLFAPLHK++KSPK RM + PS+LTP KL RE S LS+ S  S +     ST  +S       P   KS  S      LK  L++K++RI+QLL + + ER+++ KAASQTD  E  L+K+ +E             T R  + + E     LK+ LE+E+++ +D++FRLEEE ++R E                  E+I+ LE ++        D  G     E        +L   EK++ +   +   M+ E++ + ++L  +  +NE+  KE+    T L   E  L++ R+      E   N+VFE+EEQ++ L +++  ++K ++ S+ SLE E+K      ES +    +S + I +LE  L  K+N +++  HK++SL+S ++ ++S     S ++    K   +LK  LTK E E++  K +L   +T++  L   V +RD  I +LE  +  I  +        K KEI+ LK+ +  +GSD QV +QEK +++  LSSKL   +  +      +  +  + +  KA V+     L  +   K + +KEI + KDS     +EV+RL+ TI  +D ++E        L S L+ L +           QL  ++   +K   ++E   +  + T  K+ K  EE I +           H++ +K L     G+L K        + K  E  LE S L     S++D   + L+ LR +  +++ +    +  + VL E +  K A +S    D+ ++   L + +++ ++      + G   +  +G    L ++K  LE  +  +Q+ + D+ + + +L   N EL +  +E       L+Q + Q +++   L  E+  K A + K            IS     LE  L+ T K+       SI    Q  + L R K   + +++E + +    K ++ DL+  L++  +T + +EK  K E  + K+ ELE  +++++ +H+EE+ E+VD+VN+L+ KE +L     +L +  K I +L  +L      H + +   +E+H K+++ + +   ES +Q  FLKE M   KVE DLQ++K + ++E +      EMD+K  +I  +KD++ +KEEELEF KEEL+AK EE  +L+TDL++KE SF+N  KE +E+HK+E+E L++K E   A++ +L+E LA+  +E D K+DE+   KK E+N ++DQ+SE++  ILD + +++ LE ++ SL S+   + ++   K+ KI+S+NE LR  K +LE++LK+L ++ D +   NE LSI++VE E QV+E +SQ Q++ V YEK Q ELTEK E  +Q   +L +LE+ L  ER RVD LQ+ L + E+ERD  ++     +K +++N   + ++ +N  L+  + E+  K  F  +    ER  L   +      L+ + EE ++  +  D++  EN QL+SY+R+V++LEQE ++LE+++VTL  ESR  + KSPSP       + DG+  L+ QVDFLNSVIVDMQRKND+LK+KLE+ E+AG++D   E  F FNGVS+R  APRLFCDICD FD+HDTEDCP QA  + E   H+K  G R  + R YC
Sbjct:    1 MAGTKTGTLKYLGPTDFAKGEWAGIELEAPIGKNDGSVDGKKYFECAANFGLFAPLHKVSKSPKMRMMK-PSALTPGKLRRESSTLSDRSTVSSIQGRGFSTPDKSDRQGLVTPATVKSNTSA-----LKEMLRDKDERINQLLKDLDLERSEVGKAASQTDEVETKLTKVQQELS-----------TCRKNVLELEGSRDQLKDLLEEERKRAEDLQFRLEEEQILRGEGRTEEESGSDSSELRVNRERIKELEIEIVTLKTQHEDAAGNGKELEEEKERSRKEL---EKVKMELEEENKVMRKELEEMKKEL-VKSEENESALKELRDKMTNL-NRELLLEQKRS------ETEANKVFEAEEQLMALNEDLVQIKKKMEVSEQSLEAEKKRSLEQIESYRTAEFQSAETIIQLETELKTKENAMMDLTHKINSLESKAAEMESKWSSASGDVNSGKKEVNELKLALTKVEDELKESKSKLFETETRINAL---VEERD--IINLEKDELVIELKNKEGLLANKEKEIESLKTNIASSGSDFQVQVQEKIQEISALSSKLSTLEVQLGQKTKAMSEKEGEISELKATVKSIQSSLDHMTELKSNCDKEIGMLKDSINGMNEEVERLNQTIKKRDDDIESANKSTDKLQSDLDTLSV-----------QLVDVKASYEKQIGDIESSFESEKLTLAKSNKGLEEEIMKFKKSFEAEVQKHEESLKELEHTKVGELKKLEKKNAANEKKIEEQALEISRLISDSQSSADHKDRELSSLRTETGELKESIKQLKSNISVLNENLAEKQAFLSEKEADMVALNTELTLVKSQVEELKKVELEAGNLRSVVEG----LKEDKSSLESQLLNLQTETTDNSSQVQSLTQSNTELSSQLSE-------LKQTLAQWKENFAKLEAEKKEKEAGLTKDLEKSRIEHEREISKLNKTLE-QLKNTCKEAEDQHNQSIEELKQNQEVLIRKK---ESLIQETEEKIEKFKTELTDLK--LRLDAATDDISEKTDKIESLEWKKSELETQLDRVKINHDEELGEMVDIVNDLKLKEDELVGVRRDLTVQNKKIDELCAQLLKQESTHKETMKRKEEEHVKKLESMEQKFAESVNQAQFLKEQMASIKVESDLQVEKVRMEMEGEIKSRNIEMDQKISIINKLKDELVSKEEELEFTKEELQAKKEEIEELSTDLDIKEVSFNNENKEIEERHKEEVEELISKHETLSAKIGELNEKLARQTSEFDYKLDELTRTKKVELNEVKDQLSEKETLILDQELAMKNLEMKLESLESTMKGDSELANTKLEKISSENESLRRSKYELETSLKTLNSERDHIVQLNEGLSIRIVEFEVQVQEMSSQYQSLSVQYEKQQVELTEKCELMSQGAGSLADLEQLLSTERARVDSLQVELNDLESERDQLQAM---IEKTNSENEKLIKINSENTELQGQVTEMTRKNEFLFEEIARERLRLESKITTLAEELEKEKEEKRNLSQSLDSVSSENSQLSSYKRQVLMLEQENKDLENKLVTLAAESRVQKTKSPSP-------LADGDENLQGQVDFLNSVIVDMQRKNDELKSKLELLENAGVLDESIE--FYFNGVSARQAAPRLFCDICDEFDLHDTEDCPKQASSVVEETGHSKHSGKRKPEPRAYC 1598          

HSP 2 Score: 90.5077 bits (223), Expect = 3.498e-14
Identity = 45/119 (37.82%), Postives = 71/119 (59.66%), Query Frame = 0

Query:   11 VGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQP-LASGPPSCLTTPRKVSKAGRASMSPSESVVDLKS-GTPSPTGS-TISLVSMAPLK 126
            + GTK+G + +LG T FA GEWAG+ L+  +GKNDG+V G  YF+CA   G+F+   ++S+ P +    PS L TP K+ +   +++S   +V  ++  G  +P  S    LV+ A +K
Sbjct:    1 MAGTKTGTLKYLGPTDFAKGEWAGIELEAPIGKNDGSVDGKKYFECAANFGLFAPLHKVSKSPKMRMMKPSAL-TPGKLRRES-STLSDRSTVSSIQGRGFSTPDKSDRQGLVTPATVK 117          

BLAST of putative restin vs. nr
Match: gi|1199381190|ref|XP_021185117.1| (restin homolog isoform X11 [Helicoverpa armigera] >gi|1199381192|ref|XP_021185118.1| restin homolog isoform X11 [Helicoverpa armigera] >gi|1199381194|ref|XP_021185119.1| restin homolog isoform X11 [Helicoverpa armigera])

HSP 1 Score: 441.81 bits (1135), Expect = 1.750e-124
Identity = 528/1867 (28.28%), Postives = 897/1867 (48.04%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPP----------SCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSLTPRKLNRE---RSDLSEASMASMMSTVSRSQVPVK--------------KKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKADLNGGKSAF---------ETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRL-VATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLK----QDLKDQLTKAESEIRSLKEEL----DGAKTKV---EGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQL-------DKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQ--DQLEQIQKD--AKKTTHELE--EKLKKSEATKEKA---EKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNLNDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQF---DDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKD------EKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEID--------------LADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFND---LNTDLEVKEASFDNSFKETDEKH---KDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILD--LKASLEELEGRVTSLNSSSADEGK-IHEAKVA--KITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVD---ELQIALAEAETERDSARSKSKDFQKISTQNVTLDEDNENLKKD--IEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRS----------GRKSPSPAKSI-SSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            +VWVGGTK G+IA++GET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C    GVFSR +RL+R PL +  P          S    P   ++  RA +SP+ S+   +S   S   ++IS  +   +++GDRVIV ++  G+K GTLR++G T+FA G W GVELD  +GKNDG V  KRYFEC P FGLFAP+ K+++SP     R+P +       R    R   S  S+ S+ ++++ S+V V+              + S   + A   L+  L+EK+  +++L+ ERE ERA++AK + Q D AE  L+++        +++  +A +  + ++   EKI  L   LEDEK+K++D+ FR EEE + R   D     + +E+ +++ +Q  +EL+A+A+L   ++           + R AD+       ++         S+++  +D     L+ +  + ++ GK +    TRL   +E  L EA+T + + + E     FE++  ++       T +  L ESK  LE E++N+  L    KE S   ID  ++L +  +  D+  LE    L+ +   S   +  L ++  +LK  L  K    + L+D   K E+  +SL+EEL    D   +KV   E L ++V Q+ E  K+   +  ++ +    K+KE ++L ++L       +K  S+ +  + E+ +++I+L    E A +       +  ++H+K A      EE  +E+ D     E L KEI   K               T++   +E E L    S  T  ++ELKL+ +++E   Q  ++L Q   D  AK +  ELE  E  ++ E+  EK    +KAK++   E      QT + ++                      +F++L + L     E+ K L EL+ K  ++     +  RA + LK++          L  I+E   V   ER + + +  +  +KL  + GK+ E   + +++   +  +Q+   D+D L+   N  LK N+ E  +V+E+ EQ +E      D + K                 KLE+D++  +++ ++LK +   ++ +     D+LT A+   +  LEE +  E +++  ++D+  +L  + +  E+    L++      E++ +++ EE   +L+   E++   + D+  +L   E+D              LA   EEL  A +S K L +KL     E    K+K+  E  V N S EE+      LKE +G               Q+E  K +    + +KT L     + +     EL   K+  E  + + ND   +N +L+      D+  K+  EK+    ++I  L T++++ + EV++  +T+ + +    +KM + +    SE   L  Q+ + + S L+   K  +E L   + +L +  A++GK + E K A  K+T           +L+  +   + D  QL+N NE        ++    +   Q+Q     Y+K  K      + +  D D  KEL+++ D     VD   +LQ  L EAE         SK  +     ++   + + +++KD  +E+L  +LH A+        +   +V+  K  +++KV++ + ++     L    +++ S + KV I E+EK  L+ ++     + R           G+   +  K+    +M DG    QV FLNSVIVDMQRKN+ L  +++  E   +    A    +FNG  +R+VAPRLFCDICDVFD HDTEDCP QA E   P        A    R YCD CEVFGH+T NC+E ETF
Sbjct:   39 RVWVGGTKPGQIAYIGETQFAPGDWAGIVLDEPIGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRVPLITHAPHDASPISDAGSVFERPPSGTRQRRA-LSPNGSI---RSIVSSKMNASISTTTNGDVRVGDRVIVSSS-RGSKAGTLRYVGVTDFATGVWAGVELDDPLGKNDGSVDGKRYFECSPRFGLFAPVSKVSRSPS---NRKPGACAIHSNGRATPLRRSNSRESLTSLGTSIASSRVGVRLGVTSLGAQRVGGPRASSTPVSAKNALQELLREKQQHLERLMRERELERAEMAKISLQVDRAETALTQL--------KKENTQATSENSKLKAELEKINKL---LEDEKQKNEDLMFRTEEENINR--EDYNKYKDAMEQEKAVREQRIRELEAEAELQTARAEATATALRMHEDQRAADIAAANQQHQEELAAAHTLSSELQKLLDEAYALLKEKENEKDSLGKSMSEELTRLKTDSEKALNEAKTKMAIAQTE-----FETQVSVL-------TAKLQLAESK--LETEKQNLERLN---KENSQIIIDLNTKLTQLQATVDDKTLE----LNKVVGVSKEHEVNLNKEITKLKMELSAKILDLEQLEDSKRKQETVFKSLQEELGRVKDEYNSKVNEYETLLSEVSQQHEKNKT---EMMELQQNLSAKTKEYEKLLAELSTSTNSSEKLVSEYKETIHERDKEIIKLKEDYEQAAA-------NFNIKHSKIA------EEHKKEIDDRNSKIEQLMKEIESHKQ--------------TLDQSKVEFEALN---SQFTLNVDELKLLKEENEKLKQSINELTQANNDLKAKMSAMELEVGEYKRQFESANEKCAELQKAKDKIESEYMNLTGQTTDSNE----------------------QFNKLSQHL----KETEKELQELKDKHREV---VNNHARAEQELKQK----------LFKIQEDFSV---ERGQLVVSVNENIEKLKEADGKIKEFETQTVEVNNRLRNLQT---DNDKLLDE-NSILK-NEIEALKVKEQ-EQNMEH-----DTICK-----------------KLEIDIDRYKEEISILKADGATSEVKLMEKVDQLTEAQNDLNNKLEEARKHEDSLQKILDDMTAQLNNQKTNFEKEISQLQNNLSTVTEETNQLKAEE--NRLKEILEQKQNNVKDLTLKLEMLEVDLKSNVEIVTEKDRQLAQVNEELNKATESKKQLEEKLNGAALETSALKQKY--ESLVANSSAEET-----VLKEQLG---------------QLEVLKKEMSTLVQEKTSLESKYSETLA----ELTTIKKNYEDAVNKINDNVTVNNELKKSVEEKDSILKQQTEKNVIDAEKISKLETELDQLKQEVSN-KDTVIQEKESQLTKMHDALQLGSSETQQLVAQL-QLENSKLNETYKTEVESLNNTLKTLQTQVAEKGKELEELKQASEKVT-----------ELQKLVDKSEQDIKQLTNINE-------AQKMNYEDLNKQLQAQFDEYKKESKRARHDLKNTLNDYD--KELKQSKDKVTAEVDKQNQLQQKLTEAE---------SKILELTQKLDLIAVQQSSDIEKDKQLEKLTVELHAAKQTSAESVAKSETLVKNLKADIENKVKDLRLKEDLIGKL---QEEVKSQKAKVEIAEREKLLLQKEMAMKGKDVRDKNDNSAMGVLGQGDTASEKTTDDKEMIDG----QVSFLNSVIVDMQRKNEQLMARVQALEGGSV----APEPVLFNGRKARAVAPRLFCDICDVFDAHDTEDCPKQAGEPDAPPPPANRRAAPPP-RPYCDICEVFGHATENCDEEETF 1689          

BLAST of putative restin vs. nr
Match: gi|1199381184|ref|XP_021185114.1| (restin homolog isoform X8 [Helicoverpa armigera])

HSP 1 Score: 441.425 bits (1134), Expect = 4.484e-124
Identity = 528/1867 (28.28%), Postives = 897/1867 (48.04%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPP----------SCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSLTPRKLNRE---RSDLSEASMASMMSTVSRSQVPVK--------------KKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKADLNGGKSAF---------ETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRL-VATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLK----QDLKDQLTKAESEIRSLKEEL----DGAKTKV---EGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQL-------DKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQ--DQLEQIQKD--AKKTTHELE--EKLKKSEATKEKA---EKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNLNDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQF---DDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKD------EKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEID--------------LADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFND---LNTDLEVKEASFDNSFKETDEKH---KDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILD--LKASLEELEGRVTSLNSSSADEGK-IHEAKVA--KITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVD---ELQIALAEAETERDSARSKSKDFQKISTQNVTLDEDNENLKKD--IEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRS----------GRKSPSPAKSI-SSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            +VWVGGTK G+IA++GET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C    GVFSR +RL+R PL +  P          S    P   ++  RA +SP+ S+   +S   S   ++IS  +   +++GDRVIV ++  G+K GTLR++G T+FA G W GVELD  +GKNDG V  KRYFEC P FGLFAP+ K+++SP     R+P +       R    R   S  S+ S+ ++++ S+V V+              + S   + A   L+  L+EK+  +++L+ ERE ERA++AK + Q D AE  L+++        +++  +A +  + ++   EKI  L   LEDEK+K++D+ FR EEE + R   D     + +E+ +++ +Q  +EL+A+A+L   ++           + R AD+       ++         S+++  +D     L+ +  + ++ GK +    TRL   +E  L EA+T + + + E     FE++  ++       T +  L ESK  LE E++N+  L    KE S   ID  ++L +  +  D+  LE    L+ +   S   +  L ++  +LK  L  K    + L+D   K E+  +SL+EEL    D   +KV   E L ++V Q+ E  K+   +  ++ +    K+KE ++L ++L       +K  S+ +  + E+ +++I+L    E A +       +  ++H+K A      EE  +E+ D     E L KEI   K               T++   +E E L    S  T  ++ELKL+ +++E   Q  ++L Q   D  AK +  ELE  E  ++ E+  EK    +KAK++   E      QT + ++                      +F++L + L     E+ K L EL+ K  ++     +  RA + LK++          L  I+E   V   ER + + +  +  +KL  + GK+ E   + +++   +  +Q+   D+D L+   N  LK N+ E  +V+E+ EQ +E      D + K                 KLE+D++  +++ ++LK +   ++ +     D+LT A+   +  LEE +  E +++  ++D+  +L  + +  E+    L++      E++ +++ EE   +L+   E++   + D+  +L   E+D              LA   EEL  A +S K L +KL     E    K+K+  E  V N S EE+      LKE +G               Q+E  K +    + +KT L     + +     EL   K+  E  + + ND   +N +L+      D+  K+  EK+    ++I  L T++++ + EV++  +T+ + +    +KM + +    SE   L  Q+ + + S L+   K  +E L   + +L +  A++GK + E K A  K+T           +L+  +   + D  QL+N NE        ++    +   Q+Q     Y+K  K      + +  D D  KEL+++ D     VD   +LQ  L EAE         SK  +     ++   + + +++KD  +E+L  +LH A+        +   +V+  K  +++KV++ + ++     L    +++ S + KV I E+EK  L+ ++     + R           G+   +  K+    +M DG    QV FLNSVIVDMQRKN+ L  +++  E   +    A    +FNG  +R+VAPRLFCDICDVFD HDTEDCP QA E   P        A    R YCD CEVFGH+T NC+E ETF
Sbjct:   99 RVWVGGTKPGQIAYIGETQFAPGDWAGIVLDEPIGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRVPLITHAPHDASPISDAGSVFERPPSGTRQRRA-LSPNGSI---RSIVSSKMNASISTTTNGDVRVGDRVIVSSS-RGSKAGTLRYVGVTDFATGVWAGVELDDPLGKNDGSVDGKRYFECSPRFGLFAPVSKVSRSPS---NRKPGACAIHSNGRATPLRRSNSRESLTSLGTSIASSRVGVRLGVTSLGAQRVGGPRASSTPVSAKNALQELLREKQQHLERLMRERELERAEMAKISLQVDRAETALTQL--------KKENTQATSENSKLKAELEKINKL---LEDEKQKNEDLMFRTEEENINR--EDYNKYKDAMEQEKAVREQRIRELEAEAELQTARAEATATALRMHEDQRAADIAAANQQHQEELAAAHTLSSELQKLLDEAYALLKEKENEKDSLGKSMSEELTRLKTDSEKALNEAKTKMAIAQTE-----FETQVSVL-------TAKLQLAESK--LETEKQNLERLN---KENSQIIIDLNTKLTQLQATVDDKTLE----LNKVVGVSKEHEVNLNKEITKLKMELSAKILDLEQLEDSKRKQETVFKSLQEELGRVKDEYNSKVNEYETLLSEVSQQHEKNKT---EMMELQQNLSAKTKEYEKLLAELSTSTNSSEKLVSEYKETIHERDKEIIKLKEDYEQAAA-------NFNIKHSKIA------EEHKKEIDDRNSKIEQLMKEIESHKQ--------------TLDQSKVEFEALN---SQFTLNVDELKLLKEENEKLKQSINELTQANNDLKAKMSAMELEVGEYKRQFESANEKCAELQKAKDKIESEYMNLTGQTTDSNE----------------------QFNKLSQHL----KETEKELQELKDKHREV---VNNHARAEQELKQK----------LFKIQEDFSV---ERGQLVVSVNENIEKLKEADGKIKEFETQTVEVNNRLRNLQT---DNDKLLDE-NSILK-NEIEALKVKEQ-EQNMEH-----DTICK-----------------KLEIDIDRYKEEISILKADGATSEVKLMEKVDQLTEAQNDLNNKLEEARKHEDSLQKILDDMTAQLNNQKTNFEKEISQLQNNLSTVTEETNQLKAEE--NRLKEILEQKQNNVKDLTLKLEMLEVDLKSNVEIVTEKDRQLAQVNEELNKATESKKQLEEKLNGAALETSALKQKY--ESLVANSSAEET-----VLKEQLG---------------QLEVLKKEMSTLVQEKTSLESKYSETLA----ELTTIKKNYEDAVNKINDNVTVNNELKKSVEEKDSILKQQTEKNVIDAEKISKLETELDQLKQEVSN-KDTVIQEKESQLTKMHDALQLGSSETQQLVAQL-QLENSKLNETYKTEVESLNNTLKTLQTQVAEKGKELEELKQASEKVT-----------ELQKLVDKSEQDIKQLTNINE-------AQKMNYEDLNKQLQAQFDEYKKESKRARHDLKNTLNDYD--KELKQSKDKVTAEVDKQNQLQQKLTEAE---------SKILELTQKLDLIAVQQSSDIEKDKQLEKLTVELHAAKQTSAESVAKSETLVKNLKADIENKVKDLRLKEDLIGKL---QEEVKSQKAKVEIAEREKLLLQKEMAMKGKDVRDKNDNSAMGVLGQGDTASEKTTDDKEMIDG----QVSFLNSVIVDMQRKNEQLMARVQALEGGSV----APEPVLFNGRKARAVAPRLFCDICDVFDAHDTEDCPKQAGEPDAPPPPANRRAAPPP-RPYCDICEVFGHATENCDEEETF 1749          

BLAST of putative restin vs. nr
Match: gi|1199381188|ref|XP_021185116.1| (restin homolog isoform X10 [Helicoverpa armigera])

HSP 1 Score: 440.654 bits (1132), Expect = 5.382e-124
Identity = 528/1867 (28.28%), Postives = 897/1867 (48.04%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPP----------SCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSLTPRKLNRE---RSDLSEASMASMMSTVSRSQVPVK--------------KKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKADLNGGKSAF---------ETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRL-VATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLK----QDLKDQLTKAESEIRSLKEEL----DGAKTKV---EGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQL-------DKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQ--DQLEQIQKD--AKKTTHELE--EKLKKSEATKEKA---EKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNLNDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQF---DDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKD------EKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEID--------------LADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFND---LNTDLEVKEASFDNSFKETDEKH---KDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILD--LKASLEELEGRVTSLNSSSADEGK-IHEAKVA--KITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVD---ELQIALAEAETERDSARSKSKDFQKISTQNVTLDEDNENLKKD--IEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRS----------GRKSPSPAKSI-SSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            +VWVGGTK G+IA++GET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C    GVFSR +RL+R PL +  P          S    P   ++  RA +SP+ S+   +S   S   ++IS  +   +++GDRVIV ++  G+K GTLR++G T+FA G W GVELD  +GKNDG V  KRYFEC P FGLFAP+ K+++SP     R+P +       R    R   S  S+ S+ ++++ S+V V+              + S   + A   L+  L+EK+  +++L+ ERE ERA++AK + Q D AE  L+++        +++  +A +  + ++   EKI  L   LEDEK+K++D+ FR EEE + R   D     + +E+ +++ +Q  +EL+A+A+L   ++           + R AD+       ++         S+++  +D     L+ +  + ++ GK +    TRL   +E  L EA+T + + + E     FE++  ++       T +  L ESK  LE E++N+  L    KE S   ID  ++L +  +  D+  LE    L+ +   S   +  L ++  +LK  L  K    + L+D   K E+  +SL+EEL    D   +KV   E L ++V Q+ E  K+   +  ++ +    K+KE ++L ++L       +K  S+ +  + E+ +++I+L    E A +       +  ++H+K A      EE  +E+ D     E L KEI   K               T++   +E E L    S  T  ++ELKL+ +++E   Q  ++L Q   D  AK +  ELE  E  ++ E+  EK    +KAK++   E      QT + ++                      +F++L + L     E+ K L EL+ K  ++     +  RA + LK++          L  I+E   V   ER + + +  +  +KL  + GK+ E   + +++   +  +Q+   D+D L+   N  LK N+ E  +V+E+ EQ +E      D + K                 KLE+D++  +++ ++LK +   ++ +     D+LT A+   +  LEE +  E +++  ++D+  +L  + +  E+    L++      E++ +++ EE   +L+   E++   + D+  +L   E+D              LA   EEL  A +S K L +KL     E    K+K+  E  V N S EE+      LKE +G               Q+E  K +    + +KT L     + +     EL   K+  E  + + ND   +N +L+      D+  K+  EK+    ++I  L T++++ + EV++  +T+ + +    +KM + +    SE   L  Q+ + + S L+   K  +E L   + +L +  A++GK + E K A  K+T           +L+  +   + D  QL+N NE        ++    +   Q+Q     Y+K  K      + +  D D  KEL+++ D     VD   +LQ  L EAE         SK  +     ++   + + +++KD  +E+L  +LH A+        +   +V+  K  +++KV++ + ++     L    +++ S + KV I E+EK  L+ ++     + R           G+   +  K+    +M DG    QV FLNSVIVDMQRKN+ L  +++  E   +    A    +FNG  +R+VAPRLFCDICDVFD HDTEDCP QA E   P        A    R YCD CEVFGH+T NC+E ETF
Sbjct:   49 RVWVGGTKPGQIAYIGETQFAPGDWAGIVLDEPIGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRVPLITHAPHDASPISDAGSVFERPPSGTRQRRA-LSPNGSI---RSIVSSKMNASISTTTNGDVRVGDRVIVSSS-RGSKAGTLRYVGVTDFATGVWAGVELDDPLGKNDGSVDGKRYFECSPRFGLFAPVSKVSRSPS---NRKPGACAIHSNGRATPLRRSNSRESLTSLGTSIASSRVGVRLGVTSLGAQRVGGPRASSTPVSAKNALQELLREKQQHLERLMRERELERAEMAKISLQVDRAETALTQL--------KKENTQATSENSKLKAELEKINKL---LEDEKQKNEDLMFRTEEENINR--EDYNKYKDAMEQEKAVREQRIRELEAEAELQTARAEATATALRMHEDQRAADIAAANQQHQEELAAAHTLSSELQKLLDEAYALLKEKENEKDSLGKSMSEELTRLKTDSEKALNEAKTKMAIAQTE-----FETQVSVL-------TAKLQLAESK--LETEKQNLERLN---KENSQIIIDLNTKLTQLQATVDDKTLE----LNKVVGVSKEHEVNLNKEITKLKMELSAKILDLEQLEDSKRKQETVFKSLQEELGRVKDEYNSKVNEYETLLSEVSQQHEKNKT---EMMELQQNLSAKTKEYEKLLAELSTSTNSSEKLVSEYKETIHERDKEIIKLKEDYEQAAA-------NFNIKHSKIA------EEHKKEIDDRNSKIEQLMKEIESHKQ--------------TLDQSKVEFEALN---SQFTLNVDELKLLKEENEKLKQSINELTQANNDLKAKMSAMELEVGEYKRQFESANEKCAELQKAKDKIESEYMNLTGQTTDSNE----------------------QFNKLSQHL----KETEKELQELKDKHREV---VNNHARAEQELKQK----------LFKIQEDFSV---ERGQLVVSVNENIEKLKEADGKIKEFETQTVEVNNRLRNLQT---DNDKLLDE-NSILK-NEIEALKVKEQ-EQNMEH-----DTICK-----------------KLEIDIDRYKEEISILKADGATSEVKLMEKVDQLTEAQNDLNNKLEEARKHEDSLQKILDDMTAQLNNQKTNFEKEISQLQNNLSTVTEETNQLKAEE--NRLKEILEQKQNNVKDLTLKLEMLEVDLKSNVEIVTEKDRQLAQVNEELNKATESKKQLEEKLNGAALETSALKQKY--ESLVANSSAEET-----VLKEQLG---------------QLEVLKKEMSTLVQEKTSLESKYSETLA----ELTTIKKNYEDAVNKINDNVTVNNELKKSVEEKDSILKQQTEKNVIDAEKISKLETELDQLKQEVSN-KDTVIQEKESQLTKMHDALQLGSSETQQLVAQL-QLENSKLNETYKTEVESLNNTLKTLQTQVAEKGKELEELKQASEKVT-----------ELQKLVDKSEQDIKQLTNINE-------AQKMNYEDLNKQLQAQFDEYKKESKRARHDLKNTLNDYD--KELKQSKDKVTAEVDKQNQLQQKLTEAE---------SKILELTQKLDLIAVQQSSDIEKDKQLEKLTVELHAAKQTSAESVAKSETLVKNLKADIENKVKDLRLKEDLIGKL---QEEVKSQKAKVEIAEREKLLLQKEMAMKGKDVRDKNDNSAMGVLGQGDTASEKTTDDKEMIDG----QVSFLNSVIVDMQRKNEQLMARVQALEGGSV----APEPVLFNGRKARAVAPRLFCDICDVFDAHDTEDCPKQAGEPDAPPPPANRRAAPPP-RPYCDICEVFGHATENCDEEETF 1699          

BLAST of putative restin vs. nr
Match: gi|1199381182|ref|XP_021185113.1| (CAP-Gly domain-containing linker protein 1 isoform X7 [Helicoverpa armigera])

HSP 1 Score: 441.039 bits (1133), Expect = 7.039e-124
Identity = 528/1867 (28.28%), Postives = 897/1867 (48.04%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPP----------SCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSLTPRKLNRE---RSDLSEASMASMMSTVSRSQVPVK--------------KKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKADLNGGKSAF---------ETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRL-VATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLK----QDLKDQLTKAESEIRSLKEEL----DGAKTKV---EGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQL-------DKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQ--DQLEQIQKD--AKKTTHELE--EKLKKSEATKEKA---EKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNLNDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQF---DDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKD------EKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEID--------------LADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFND---LNTDLEVKEASFDNSFKETDEKH---KDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILD--LKASLEELEGRVTSLNSSSADEGK-IHEAKVA--KITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVD---ELQIALAEAETERDSARSKSKDFQKISTQNVTLDEDNENLKKD--IEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRS----------GRKSPSPAKSI-SSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            +VWVGGTK G+IA++GET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C    GVFSR +RL+R PL +  P          S    P   ++  RA +SP+ S+   +S   S   ++IS  +   +++GDRVIV ++  G+K GTLR++G T+FA G W GVELD  +GKNDG V  KRYFEC P FGLFAP+ K+++SP     R+P +       R    R   S  S+ S+ ++++ S+V V+              + S   + A   L+  L+EK+  +++L+ ERE ERA++AK + Q D AE  L+++        +++  +A +  + ++   EKI  L   LEDEK+K++D+ FR EEE + R   D     + +E+ +++ +Q  +EL+A+A+L   ++           + R AD+       ++         S+++  +D     L+ +  + ++ GK +    TRL   +E  L EA+T + + + E     FE++  ++       T +  L ESK  LE E++N+  L    KE S   ID  ++L +  +  D+  LE    L+ +   S   +  L ++  +LK  L  K    + L+D   K E+  +SL+EEL    D   +KV   E L ++V Q+ E  K+   +  ++ +    K+KE ++L ++L       +K  S+ +  + E+ +++I+L    E A +       +  ++H+K A      EE  +E+ D     E L KEI   K               T++   +E E L    S  T  ++ELKL+ +++E   Q  ++L Q   D  AK +  ELE  E  ++ E+  EK    +KAK++   E      QT + ++                      +F++L + L     E+ K L EL+ K  ++     +  RA + LK++          L  I+E   V   ER + + +  +  +KL  + GK+ E   + +++   +  +Q+   D+D L+   N  LK N+ E  +V+E+ EQ +E      D + K                 KLE+D++  +++ ++LK +   ++ +     D+LT A+   +  LEE +  E +++  ++D+  +L  + +  E+    L++      E++ +++ EE   +L+   E++   + D+  +L   E+D              LA   EEL  A +S K L +KL     E    K+K+  E  V N S EE+      LKE +G               Q+E  K +    + +KT L     + +     EL   K+  E  + + ND   +N +L+      D+  K+  EK+    ++I  L T++++ + EV++  +T+ + +    +KM + +    SE   L  Q+ + + S L+   K  +E L   + +L +  A++GK + E K A  K+T           +L+  +   + D  QL+N NE        ++    +   Q+Q     Y+K  K      + +  D D  KEL+++ D     VD   +LQ  L EAE         SK  +     ++   + + +++KD  +E+L  +LH A+        +   +V+  K  +++KV++ + ++     L    +++ S + KV I E+EK  L+ ++     + R           G+   +  K+    +M DG    QV FLNSVIVDMQRKN+ L  +++  E   +    A    +FNG  +R+VAPRLFCDICDVFD HDTEDCP QA E   P        A    R YCD CEVFGH+T NC+E ETF
Sbjct:  104 RVWVGGTKPGQIAYIGETQFAPGDWAGIVLDEPIGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRVPLITHAPHDASPISDAGSVFERPPSGTRQRRA-LSPNGSI---RSIVSSKMNASISTTTNGDVRVGDRVIVSSS-RGSKAGTLRYVGVTDFATGVWAGVELDDPLGKNDGSVDGKRYFECSPRFGLFAPVSKVSRSPS---NRKPGACAIHSNGRATPLRRSNSRESLTSLGTSIASSRVGVRLGVTSLGAQRVGGPRASSTPVSAKNALQELLREKQQHLERLMRERELERAEMAKISLQVDRAETALTQL--------KKENTQATSENSKLKAELEKINKL---LEDEKQKNEDLMFRTEEENINR--EDYNKYKDAMEQEKAVREQRIRELEAEAELQTARAEATATALRMHEDQRAADIAAANQQHQEELAAAHTLSSELQKLLDEAYALLKEKENEKDSLGKSMSEELTRLKTDSEKALNEAKTKMAIAQTE-----FETQVSVL-------TAKLQLAESK--LETEKQNLERLN---KENSQIIIDLNTKLTQLQATVDDKTLE----LNKVVGVSKEHEVNLNKEITKLKMELSAKILDLEQLEDSKRKQETVFKSLQEELGRVKDEYNSKVNEYETLLSEVSQQHEKNKT---EMMELQQNLSAKTKEYEKLLAELSTSTNSSEKLVSEYKETIHERDKEIIKLKEDYEQAAA-------NFNIKHSKIA------EEHKKEIDDRNSKIEQLMKEIESHKQ--------------TLDQSKVEFEALN---SQFTLNVDELKLLKEENEKLKQSINELTQANNDLKAKMSAMELEVGEYKRQFESANEKCAELQKAKDKIESEYMNLTGQTTDSNE----------------------QFNKLSQHL----KETEKELQELKDKHREV---VNNHARAEQELKQK----------LFKIQEDFSV---ERGQLVVSVNENIEKLKEADGKIKEFETQTVEVNNRLRNLQT---DNDKLLDE-NSILK-NEIEALKVKEQ-EQNMEH-----DTICK-----------------KLEIDIDRYKEEISILKADGATSEVKLMEKVDQLTEAQNDLNNKLEEARKHEDSLQKILDDMTAQLNNQKTNFEKEISQLQNNLSTVTEETNQLKAEE--NRLKEILEQKQNNVKDLTLKLEMLEVDLKSNVEIVTEKDRQLAQVNEELNKATESKKQLEEKLNGAALETSALKQKY--ESLVANSSAEET-----VLKEQLG---------------QLEVLKKEMSTLVQEKTSLESKYSETLA----ELTTIKKNYEDAVNKINDNVTVNNELKKSVEEKDSILKQQTEKNVIDAEKISKLETELDQLKQEVSN-KDTVIQEKESQLTKMHDALQLGSSETQQLVAQL-QLENSKLNETYKTEVESLNNTLKTLQTQVAEKGKELEELKQASEKVT-----------ELQKLVDKSEQDIKQLTNINE-------AQKMNYEDLNKQLQAQFDEYKKESKRARHDLKNTLNDYD--KELKQSKDKVTAEVDKQNQLQQKLTEAE---------SKILELTQKLDLIAVQQSSDIEKDKQLEKLTVELHAAKQTSAESVAKSETLVKNLKADIENKVKDLRLKEDLIGKL---QEEVKSQKAKVEIAEREKLLLQKEMAMKGKDVRDKNDNSAMGVLGQGDTASEKTTDDKEMIDG----QVSFLNSVIVDMQRKNEQLMARVQALEGGSV----APEPVLFNGRKARAVAPRLFCDICDVFDAHDTEDCPKQAGEPDAPPPPANRRAAPPP-RPYCDICEVFGHATENCDEEETF 1754          

BLAST of putative restin vs. nr
Match: gi|1199381186|ref|XP_021185115.1| (restin homolog isoform X9 [Helicoverpa armigera])

HSP 1 Score: 440.654 bits (1132), Expect = 7.074e-124
Identity = 528/1867 (28.28%), Postives = 897/1867 (48.04%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPP----------SCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSLTPRKLNRE---RSDLSEASMASMMSTVSRSQVPVK--------------KKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKADLNGGKSAF---------ETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRL-VATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLK----QDLKDQLTKAESEIRSLKEEL----DGAKTKV---EGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQL-------DKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQ--DQLEQIQKD--AKKTTHELE--EKLKKSEATKEKA---EKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNLNDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQF---DDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKD------EKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEID--------------LADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFND---LNTDLEVKEASFDNSFKETDEKH---KDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILD--LKASLEELEGRVTSLNSSSADEGK-IHEAKVA--KITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVD---ELQIALAEAETERDSARSKSKDFQKISTQNVTLDEDNENLKKD--IEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRS----------GRKSPSPAKSI-SSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            +VWVGGTK G+IA++GET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C    GVFSR +RL+R PL +  P          S    P   ++  RA +SP+ S+   +S   S   ++IS  +   +++GDRVIV ++  G+K GTLR++G T+FA G W GVELD  +GKNDG V  KRYFEC P FGLFAP+ K+++SP     R+P +       R    R   S  S+ S+ ++++ S+V V+              + S   + A   L+  L+EK+  +++L+ ERE ERA++AK + Q D AE  L+++        +++  +A +  + ++   EKI  L   LEDEK+K++D+ FR EEE + R   D     + +E+ +++ +Q  +EL+A+A+L   ++           + R AD+       ++         S+++  +D     L+ +  + ++ GK +    TRL   +E  L EA+T + + + E     FE++  ++       T +  L ESK  LE E++N+  L    KE S   ID  ++L +  +  D+  LE    L+ +   S   +  L ++  +LK  L  K    + L+D   K E+  +SL+EEL    D   +KV   E L ++V Q+ E  K+   +  ++ +    K+KE ++L ++L       +K  S+ +  + E+ +++I+L    E A +       +  ++H+K A      EE  +E+ D     E L KEI   K               T++   +E E L    S  T  ++ELKL+ +++E   Q  ++L Q   D  AK +  ELE  E  ++ E+  EK    +KAK++   E      QT + ++                      +F++L + L     E+ K L EL+ K  ++     +  RA + LK++          L  I+E   V   ER + + +  +  +KL  + GK+ E   + +++   +  +Q+   D+D L+   N  LK N+ E  +V+E+ EQ +E      D + K                 KLE+D++  +++ ++LK +   ++ +     D+LT A+   +  LEE +  E +++  ++D+  +L  + +  E+    L++      E++ +++ EE   +L+   E++   + D+  +L   E+D              LA   EEL  A +S K L +KL     E    K+K+  E  V N S EE+      LKE +G               Q+E  K +    + +KT L     + +     EL   K+  E  + + ND   +N +L+      D+  K+  EK+    ++I  L T++++ + EV++  +T+ + +    +KM + +    SE   L  Q+ + + S L+   K  +E L   + +L +  A++GK + E K A  K+T           +L+  +   + D  QL+N NE        ++    +   Q+Q     Y+K  K      + +  D D  KEL+++ D     VD   +LQ  L EAE         SK  +     ++   + + +++KD  +E+L  +LH A+        +   +V+  K  +++KV++ + ++     L    +++ S + KV I E+EK  L+ ++     + R           G+   +  K+    +M DG    QV FLNSVIVDMQRKN+ L  +++  E   +    A    +FNG  +R+VAPRLFCDICDVFD HDTEDCP QA E   P        A    R YCD CEVFGH+T NC+E ETF
Sbjct:   79 RVWVGGTKPGQIAYIGETQFAPGDWAGIVLDEPIGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRVPLITHAPHDASPISDAGSVFERPPSGTRQRRA-LSPNGSI---RSIVSSKMNASISTTTNGDVRVGDRVIVSSS-RGSKAGTLRYVGVTDFATGVWAGVELDDPLGKNDGSVDGKRYFECSPRFGLFAPVSKVSRSPS---NRKPGACAIHSNGRATPLRRSNSRESLTSLGTSIASSRVGVRLGVTSLGAQRVGGPRASSTPVSAKNALQELLREKQQHLERLMRERELERAEMAKISLQVDRAETALTQL--------KKENTQATSENSKLKAELEKINKL---LEDEKQKNEDLMFRTEEENINR--EDYNKYKDAMEQEKAVREQRIRELEAEAELQTARAEATATALRMHEDQRAADIAAANQQHQEELAAAHTLSSELQKLLDEAYALLKEKENEKDSLGKSMSEELTRLKTDSEKALNEAKTKMAIAQTE-----FETQVSVL-------TAKLQLAESK--LETEKQNLERLN---KENSQIIIDLNTKLTQLQATVDDKTLE----LNKVVGVSKEHEVNLNKEITKLKMELSAKILDLEQLEDSKRKQETVFKSLQEELGRVKDEYNSKVNEYETLLSEVSQQHEKNKT---EMMELQQNLSAKTKEYEKLLAELSTSTNSSEKLVSEYKETIHERDKEIIKLKEDYEQAAA-------NFNIKHSKIA------EEHKKEIDDRNSKIEQLMKEIESHKQ--------------TLDQSKVEFEALN---SQFTLNVDELKLLKEENEKLKQSINELTQANNDLKAKMSAMELEVGEYKRQFESANEKCAELQKAKDKIESEYMNLTGQTTDSNE----------------------QFNKLSQHL----KETEKELQELKDKHREV---VNNHARAEQELKQK----------LFKIQEDFSV---ERGQLVVSVNENIEKLKEADGKIKEFETQTVEVNNRLRNLQT---DNDKLLDE-NSILK-NEIEALKVKEQ-EQNMEH-----DTICK-----------------KLEIDIDRYKEEISILKADGATSEVKLMEKVDQLTEAQNDLNNKLEEARKHEDSLQKILDDMTAQLNNQKTNFEKEISQLQNNLSTVTEETNQLKAEE--NRLKEILEQKQNNVKDLTLKLEMLEVDLKSNVEIVTEKDRQLAQVNEELNKATESKKQLEEKLNGAALETSALKQKY--ESLVANSSAEET-----VLKEQLG---------------QLEVLKKEMSTLVQEKTSLESKYSETLA----ELTTIKKNYEDAVNKINDNVTVNNELKKSVEEKDSILKQQTEKNVIDAEKISKLETELDQLKQEVSN-KDTVIQEKESQLTKMHDALQLGSSETQQLVAQL-QLENSKLNETYKTEVESLNNTLKTLQTQVAEKGKELEELKQASEKVT-----------ELQKLVDKSEQDIKQLTNINE-------AQKMNYEDLNKQLQAQFDEYKKESKRARHDLKNTLNDYD--KELKQSKDKVTAEVDKQNQLQQKLTEAE---------SKILELTQKLDLIAVQQSSDIEKDKQLEKLTVELHAAKQTSAESVAKSETLVKNLKADIENKVKDLRLKEDLIGKL---QEEVKSQKAKVEIAEREKLLLQKEMAMKGKDVRDKNDNSAMGVLGQGDTASEKTTDDKEMIDG----QVSFLNSVIVDMQRKNEQLMARVQALEGGSV----APEPVLFNGRKARAVAPRLFCDICDVFDAHDTEDCPKQAGEPDAPPPPANRRAAPPP-RPYCDICEVFGHATENCDEEETF 1729          

BLAST of putative restin vs. nr
Match: gi|1199381172|ref|XP_021185108.1| (restin homolog isoform X2 [Helicoverpa armigera])

HSP 1 Score: 440.654 bits (1132), Expect = 1.374e-123
Identity = 528/1853 (28.49%), Postives = 896/1853 (48.35%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPP----------SCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSLTPRKLNRE---RSDLSEASMASMMSTVSRSQVPVKKKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKADLNGGKSAF---------ETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRL-VATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLK----QDLKDQLTKAESEIRSLKEEL----DGAKTKV---EGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQL-------DKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQ--DQLEQIQKD--AKKTTHELE--EKLKKSEATKEKA---EKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNLNDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQF---DDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKD------EKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEID--------------LADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFND---LNTDLEVKEASFDNSFKETDEKH---KDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILD--LKASLEELEGRVTSLNSSSADEGK-IHEAKVA--KITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVD---ELQIALAEAETERDSARSKSKDFQKISTQNVTLDEDNENLKKD--IEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRS----------GRKSPSPAKSI-SSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            +VWVGGTK G+IA++GET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C    GVFSR +RL+R PL +  P          S    P   ++  RA +SP+ S+   +S   S   ++IS  +   +++GDRVIV ++  G+K GTLR++G T+FA G W GVELD  +GKNDG V  KRYFEC P FGLFAP+ K+++SP     R+P +       R    R   S  S+ S+ ++++ S+V V +  + S+ A E     L+EK+  +++L+ ERE ERA++AK + Q D AE  L+++        +++  +A +  + ++   EKI  L   LEDEK+K++D+ FR EEE + R   D     + +E+ +++ +Q  +EL+A+A+L   ++           + R AD+       ++         S+++  +D     L+ +  + ++ GK +    TRL   +E  L EA+T + + + E     FE++  ++       T +  L ESK  LE E++N+  L    KE S   ID  ++L +  +  D+  LE    L+ +   S   +  L ++  +LK  L  K    + L+D   K E+  +SL+EEL    D   +KV   E L ++V Q+ E  K+   +  ++ +    K+KE ++L ++L       +K  S+ +  + E+ +++I+L    E A +       +  ++H+K A      EE  +E+ D     E L KEI   K               T++   +E E L    S  T  ++ELKL+ +++E   Q  ++L Q   D  AK +  ELE  E  ++ E+  EK    +KAK++   E      QT + ++                      +F++L + L     E+ K L EL+ K  ++     +  RA + LK++          L  I+E   V   ER + + +  +  +KL  + GK+ E   + +++   +  +Q+   D+D L+   N  LK N+ E  +V+E+ EQ +E      D + K                 KLE+D++  +++ ++LK +   ++ +     D+LT A+   +  LEE +  E +++  ++D+  +L  + +  E+    L++      E++ +++ EE   +L+   E++   + D+  +L   E+D              LA   EEL  A +S K L +KL     E    K+K+  E  V N S EE+      LKE +G               Q+E  K +    + +KT L     + +     EL   K+  E  + + ND   +N +L+      D+  K+  EK+    ++I  L T++++ + EV++  +T+ + +    +KM + +    SE   L  Q+ + + S L+   K  +E L   + +L +  A++GK + E K A  K+T           +L+  +   + D  QL+N NE        ++    +   Q+Q     Y+K  K      + +  D D  KEL+++ D     VD   +LQ  L EAE         SK  +     ++   + + +++KD  +E+L  +LH A+        +   +V+  K  +++KV++ + ++     L    +++ S + KV I E+EK  L+ ++     + R           G+   +  K+    +M DG    QV FLNSVIVDMQRKN+ L  +++  E   +    A    +FNG  +R+VAPRLFCDICDVFD HDTEDCP QA E   P        A    R YCD CEVFGH+T NC+E ETF
Sbjct:  175 RVWVGGTKPGQIAYIGETQFAPGDWAGIVLDEPIGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRVPLITHAPHDASPISDAGSVFERPPSGTRQRRA-LSPNGSI---RSIVSSKMNASISTTTNGDVRVGDRVIVSSS-RGSKAGTLRYVGVTDFATGVWAGVELDDPLGKNDGSVDGKRYFECSPRFGLFAPVSKVSRSPS---NRKPGACAIHSNGRATPLRRSNSRESLTSLGTSIASSRVGV-RLGVTSLGAQEL----LREKQQHLERLMRERELERAEMAKISLQVDRAETALTQL--------KKENTQATSENSKLKAELEKINKL---LEDEKQKNEDLMFRTEEENINR--EDYNKYKDAMEQEKAVREQRIRELEAEAELQTARAEATATALRMHEDQRAADIAAANQQHQEELAAAHTLSSELQKLLDEAYALLKEKENEKDSLGKSMSEELTRLKTDSEKALNEAKTKMAIAQTE-----FETQVSVL-------TAKLQLAESK--LETEKQNLERLN---KENSQIIIDLNTKLTQLQATVDDKTLE----LNKVVGVSKEHEVNLNKEITKLKMELSAKILDLEQLEDSKRKQETVFKSLQEELGRVKDEYNSKVNEYETLLSEVSQQHEKNKT---EMMELQQNLSAKTKEYEKLLAELSTSTNSSEKLVSEYKETIHERDKEIIKLKEDYEQAAA-------NFNIKHSKIA------EEHKKEIDDRNSKIEQLMKEIESHKQ--------------TLDQSKVEFEALN---SQFTLNVDELKLLKEENEKLKQSINELTQANNDLKAKMSAMELEVGEYKRQFESANEKCAELQKAKDKIESEYMNLTGQTTDSNE----------------------QFNKLSQHL----KETEKELQELKDKHREV---VNNHARAEQELKQK----------LFKIQEDFSV---ERGQLVVSVNENIEKLKEADGKIKEFETQTVEVNNRLRNLQT---DNDKLLDE-NSILK-NEIEALKVKEQ-EQNMEH-----DTICK-----------------KLEIDIDRYKEEISILKADGATSEVKLMEKVDQLTEAQNDLNNKLEEARKHEDSLQKILDDMTAQLNNQKTNFEKEISQLQNNLSTVTEETNQLKAEE--NRLKEILEQKQNNVKDLTLKLEMLEVDLKSNVEIVTEKDRQLAQVNEELNKATESKKQLEEKLNGAALETSALKQKY--ESLVANSSAEET-----VLKEQLG---------------QLEVLKKEMSTLVQEKTSLESKYSETLA----ELTTIKKNYEDAVNKINDNVTVNNELKKSVEEKDSILKQQTEKNVIDAEKISKLETELDQLKQEVSN-KDTVIQEKESQLTKMHDALQLGSSETQQLVAQL-QLENSKLNETYKTEVESLNNTLKTLQTQVAEKGKELEELKQASEKVT-----------ELQKLVDKSEQDIKQLTNINE-------AQKMNYEDLNKQLQAQFDEYKKESKRARHDLKNTLNDYD--KELKQSKDKVTAEVDKQNQLQQKLTEAE---------SKILELTQKLDLIAVQQSSDIEKDKQLEKLTVELHAAKQTSAESVAKSETLVKNLKADIENKVKDLRLKEDLIGKL---QEEVKSQKAKVEIAEREKLLLQKEMAMKGKDVRDKNDNSAMGVLGQGDTASEKTTDDKEMIDG----QVSFLNSVIVDMQRKNEQLMARVQALEGGSV----APEPVLFNGRKARAVAPRLFCDICDVFDAHDTEDCPKQAGEPDAPPPPANRRAAPPP-RPYCDICEVFGHATENCDEEETF 1806          

BLAST of putative restin vs. nr
Match: gi|1199381174|ref|XP_021185109.1| (restin homolog isoform X3 [Helicoverpa armigera])

HSP 1 Score: 440.269 bits (1131), Expect = 1.517e-123
Identity = 528/1867 (28.28%), Postives = 897/1867 (48.04%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPP----------SCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSLTPRKLNRE---RSDLSEASMASMMSTVSRSQVPVK--------------KKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKADLNGGKSAF---------ETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRL-VATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLK----QDLKDQLTKAESEIRSLKEEL----DGAKTKV---EGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQL-------DKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQ--DQLEQIQKD--AKKTTHELE--EKLKKSEATKEKA---EKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNLNDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQF---DDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKD------EKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEID--------------LADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFND---LNTDLEVKEASFDNSFKETDEKH---KDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILD--LKASLEELEGRVTSLNSSSADEGK-IHEAKVA--KITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVD---ELQIALAEAETERDSARSKSKDFQKISTQNVTLDEDNENLKKD--IEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRS----------GRKSPSPAKSI-SSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            +VWVGGTK G+IA++GET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C    GVFSR +RL+R PL +  P          S    P   ++  RA +SP+ S+   +S   S   ++IS  +   +++GDRVIV ++  G+K GTLR++G T+FA G W GVELD  +GKNDG V  KRYFEC P FGLFAP+ K+++SP     R+P +       R    R   S  S+ S+ ++++ S+V V+              + S   + A   L+  L+EK+  +++L+ ERE ERA++AK + Q D AE  L+++        +++  +A +  + ++   EKI  L   LEDEK+K++D+ FR EEE + R   D     + +E+ +++ +Q  +EL+A+A+L   ++           + R AD+       ++         S+++  +D     L+ +  + ++ GK +    TRL   +E  L EA+T + + + E     FE++  ++       T +  L ESK  LE E++N+  L    KE S   ID  ++L +  +  D+  LE    L+ +   S   +  L ++  +LK  L  K    + L+D   K E+  +SL+EEL    D   +KV   E L ++V Q+ E  K+   +  ++ +    K+KE ++L ++L       +K  S+ +  + E+ +++I+L    E A +       +  ++H+K A      EE  +E+ D     E L KEI   K               T++   +E E L    S  T  ++ELKL+ +++E   Q  ++L Q   D  AK +  ELE  E  ++ E+  EK    +KAK++   E      QT + ++                      +F++L + L     E+ K L EL+ K  ++     +  RA + LK++          L  I+E   V   ER + + +  +  +KL  + GK+ E   + +++   +  +Q+   D+D L+   N  LK N+ E  +V+E+ EQ +E      D + K                 KLE+D++  +++ ++LK +   ++ +     D+LT A+   +  LEE +  E +++  ++D+  +L  + +  E+    L++      E++ +++ EE   +L+   E++   + D+  +L   E+D              LA   EEL  A +S K L +KL     E    K+K+  E  V N S EE+      LKE +G               Q+E  K +    + +KT L     + +     EL   K+  E  + + ND   +N +L+      D+  K+  EK+    ++I  L T++++ + EV++  +T+ + +    +KM + +    SE   L  Q+ + + S L+   K  +E L   + +L +  A++GK + E K A  K+T           +L+  +   + D  QL+N NE        ++    +   Q+Q     Y+K  K      + +  D D  KEL+++ D     VD   +LQ  L EAE         SK  +     ++   + + +++KD  +E+L  +LH A+        +   +V+  K  +++KV++ + ++     L    +++ S + KV I E+EK  L+ ++     + R           G+   +  K+    +M DG    QV FLNSVIVDMQRKN+ L  +++  E   +    A    +FNG  +R+VAPRLFCDICDVFD HDTEDCP QA E   P        A    R YCD CEVFGH+T NC+E ETF
Sbjct:  150 RVWVGGTKPGQIAYIGETQFAPGDWAGIVLDEPIGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRVPLITHAPHDASPISDAGSVFERPPSGTRQRRA-LSPNGSI---RSIVSSKMNASISTTTNGDVRVGDRVIVSSS-RGSKAGTLRYVGVTDFATGVWAGVELDDPLGKNDGSVDGKRYFECSPRFGLFAPVSKVSRSPS---NRKPGACAIHSNGRATPLRRSNSRESLTSLGTSIASSRVGVRLGVTSLGAQRVGGPRASSTPVSAKNALQELLREKQQHLERLMRERELERAEMAKISLQVDRAETALTQL--------KKENTQATSENSKLKAELEKINKL---LEDEKQKNEDLMFRTEEENINR--EDYNKYKDAMEQEKAVREQRIRELEAEAELQTARAEATATALRMHEDQRAADIAAANQQHQEELAAAHTLSSELQKLLDEAYALLKEKENEKDSLGKSMSEELTRLKTDSEKALNEAKTKMAIAQTE-----FETQVSVL-------TAKLQLAESK--LETEKQNLERLN---KENSQIIIDLNTKLTQLQATVDDKTLE----LNKVVGVSKEHEVNLNKEITKLKMELSAKILDLEQLEDSKRKQETVFKSLQEELGRVKDEYNSKVNEYETLLSEVSQQHEKNKT---EMMELQQNLSAKTKEYEKLLAELSTSTNSSEKLVSEYKETIHERDKEIIKLKEDYEQAAA-------NFNIKHSKIA------EEHKKEIDDRNSKIEQLMKEIESHKQ--------------TLDQSKVEFEALN---SQFTLNVDELKLLKEENEKLKQSINELTQANNDLKAKMSAMELEVGEYKRQFESANEKCAELQKAKDKIESEYMNLTGQTTDSNE----------------------QFNKLSQHL----KETEKELQELKDKHREV---VNNHARAEQELKQK----------LFKIQEDFSV---ERGQLVVSVNENIEKLKEADGKIKEFETQTVEVNNRLRNLQT---DNDKLLDE-NSILK-NEIEALKVKEQ-EQNMEH-----DTICK-----------------KLEIDIDRYKEEISILKADGATSEVKLMEKVDQLTEAQNDLNNKLEEARKHEDSLQKILDDMTAQLNNQKTNFEKEISQLQNNLSTVTEETNQLKAEE--NRLKEILEQKQNNVKDLTLKLEMLEVDLKSNVEIVTEKDRQLAQVNEELNKATESKKQLEEKLNGAALETSALKQKY--ESLVANSSAEET-----VLKEQLG---------------QLEVLKKEMSTLVQEKTSLESKYSETLA----ELTTIKKNYEDAVNKINDNVTVNNELKKSVEEKDSILKQQTEKNVIDAEKISKLETELDQLKQEVSN-KDTVIQEKESQLTKMHDALQLGSSETQQLVAQL-QLENSKLNETYKTEVESLNNTLKTLQTQVAEKGKELEELKQASEKVT-----------ELQKLVDKSEQDIKQLTNINE-------AQKMNYEDLNKQLQAQFDEYKKESKRARHDLKNTLNDYD--KELKQSKDKVTAEVDKQNQLQQKLTEAE---------SKILELTQKLDLIAVQQSSDIEKDKQLEKLTVELHAAKQTSAESVAKSETLVKNLKADIENKVKDLRLKEDLIGKL---QEEVKSQKAKVEIAEREKLLLQKEMAMKGKDVRDKNDNSAMGVLGQGDTASEKTTDDKEMIDG----QVSFLNSVIVDMQRKNEQLMARVQALEGGSV----APEPVLFNGRKARAVAPRLFCDICDVFDAHDTEDCPKQAGEPDAPPPPANRRAAPPP-RPYCDICEVFGHATENCDEEETF 1800          

BLAST of putative restin vs. nr
Match: gi|1199381180|ref|XP_021185112.1| (restin homolog isoform X6 [Helicoverpa armigera])

HSP 1 Score: 439.499 bits (1129), Expect = 2.082e-123
Identity = 528/1867 (28.28%), Postives = 897/1867 (48.04%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPP----------SCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSLTPRKLNRE---RSDLSEASMASMMSTVSRSQVPVK--------------KKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKADLNGGKSAF---------ETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRL-VATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLK----QDLKDQLTKAESEIRSLKEEL----DGAKTKV---EGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQL-------DKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQ--DQLEQIQKD--AKKTTHELE--EKLKKSEATKEKA---EKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNLNDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQF---DDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKD------EKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEID--------------LADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFND---LNTDLEVKEASFDNSFKETDEKH---KDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILD--LKASLEELEGRVTSLNSSSADEGK-IHEAKVA--KITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVD---ELQIALAEAETERDSARSKSKDFQKISTQNVTLDEDNENLKKD--IEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRS----------GRKSPSPAKSI-SSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            +VWVGGTK G+IA++GET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C    GVFSR +RL+R PL +  P          S    P   ++  RA +SP+ S+   +S   S   ++IS  +   +++GDRVIV ++  G+K GTLR++G T+FA G W GVELD  +GKNDG V  KRYFEC P FGLFAP+ K+++SP     R+P +       R    R   S  S+ S+ ++++ S+V V+              + S   + A   L+  L+EK+  +++L+ ERE ERA++AK + Q D AE  L+++        +++  +A +  + ++   EKI  L   LEDEK+K++D+ FR EEE + R   D     + +E+ +++ +Q  +EL+A+A+L   ++           + R AD+       ++         S+++  +D     L+ +  + ++ GK +    TRL   +E  L EA+T + + + E     FE++  ++       T +  L ESK  LE E++N+  L    KE S   ID  ++L +  +  D+  LE    L+ +   S   +  L ++  +LK  L  K    + L+D   K E+  +SL+EEL    D   +KV   E L ++V Q+ E  K+   +  ++ +    K+KE ++L ++L       +K  S+ +  + E+ +++I+L    E A +       +  ++H+K A      EE  +E+ D     E L KEI   K               T++   +E E L    S  T  ++ELKL+ +++E   Q  ++L Q   D  AK +  ELE  E  ++ E+  EK    +KAK++   E      QT + ++                      +F++L + L     E+ K L EL+ K  ++     +  RA + LK++          L  I+E   V   ER + + +  +  +KL  + GK+ E   + +++   +  +Q+   D+D L+   N  LK N+ E  +V+E+ EQ +E      D + K                 KLE+D++  +++ ++LK +   ++ +     D+LT A+   +  LEE +  E +++  ++D+  +L  + +  E+    L++      E++ +++ EE   +L+   E++   + D+  +L   E+D              LA   EEL  A +S K L +KL     E    K+K+  E  V N S EE+      LKE +G               Q+E  K +    + +KT L     + +     EL   K+  E  + + ND   +N +L+      D+  K+  EK+    ++I  L T++++ + EV++  +T+ + +    +KM + +    SE   L  Q+ + + S L+   K  +E L   + +L +  A++GK + E K A  K+T           +L+  +   + D  QL+N NE        ++    +   Q+Q     Y+K  K      + +  D D  KEL+++ D     VD   +LQ  L EAE         SK  +     ++   + + +++KD  +E+L  +LH A+        +   +V+  K  +++KV++ + ++     L    +++ S + KV I E+EK  L+ ++     + R           G+   +  K+    +M DG    QV FLNSVIVDMQRKN+ L  +++  E   +    A    +FNG  +R+VAPRLFCDICDVFD HDTEDCP QA E   P        A    R YCD CEVFGH+T NC+E ETF
Sbjct:  120 RVWVGGTKPGQIAYIGETQFAPGDWAGIVLDEPIGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRVPLITHAPHDASPISDAGSVFERPPSGTRQRRA-LSPNGSI---RSIVSSKMNASISTTTNGDVRVGDRVIVSSS-RGSKAGTLRYVGVTDFATGVWAGVELDDPLGKNDGSVDGKRYFECSPRFGLFAPVSKVSRSPS---NRKPGACAIHSNGRATPLRRSNSRESLTSLGTSIASSRVGVRLGVTSLGAQRVGGPRASSTPVSAKNALQELLREKQQHLERLMRERELERAEMAKISLQVDRAETALTQL--------KKENTQATSENSKLKAELEKINKL---LEDEKQKNEDLMFRTEEENINR--EDYNKYKDAMEQEKAVREQRIRELEAEAELQTARAEATATALRMHEDQRAADIAAANQQHQEELAAAHTLSSELQKLLDEAYALLKEKENEKDSLGKSMSEELTRLKTDSEKALNEAKTKMAIAQTE-----FETQVSVL-------TAKLQLAESK--LETEKQNLERLN---KENSQIIIDLNTKLTQLQATVDDKTLE----LNKVVGVSKEHEVNLNKEITKLKMELSAKILDLEQLEDSKRKQETVFKSLQEELGRVKDEYNSKVNEYETLLSEVSQQHEKNKT---EMMELQQNLSAKTKEYEKLLAELSTSTNSSEKLVSEYKETIHERDKEIIKLKEDYEQAAA-------NFNIKHSKIA------EEHKKEIDDRNSKIEQLMKEIESHKQ--------------TLDQSKVEFEALN---SQFTLNVDELKLLKEENEKLKQSINELTQANNDLKAKMSAMELEVGEYKRQFESANEKCAELQKAKDKIESEYMNLTGQTTDSNE----------------------QFNKLSQHL----KETEKELQELKDKHREV---VNNHARAEQELKQK----------LFKIQEDFSV---ERGQLVVSVNENIEKLKEADGKIKEFETQTVEVNNRLRNLQT---DNDKLLDE-NSILK-NEIEALKVKEQ-EQNMEH-----DTICK-----------------KLEIDIDRYKEEISILKADGATSEVKLMEKVDQLTEAQNDLNNKLEEARKHEDSLQKILDDMTAQLNNQKTNFEKEISQLQNNLSTVTEETNQLKAEE--NRLKEILEQKQNNVKDLTLKLEMLEVDLKSNVEIVTEKDRQLAQVNEELNKATESKKQLEEKLNGAALETSALKQKY--ESLVANSSAEET-----VLKEQLG---------------QLEVLKKEMSTLVQEKTSLESKYSETLA----ELTTIKKNYEDAVNKINDNVTVNNELKKSVEEKDSILKQQTEKNVIDAEKISKLETELDQLKQEVSN-KDTVIQEKESQLTKMHDALQLGSSETQQLVAQL-QLENSKLNETYKTEVESLNNTLKTLQTQVAEKGKELEELKQASEKVT-----------ELQKLVDKSEQDIKQLTNINE-------AQKMNYEDLNKQLQAQFDEYKKESKRARHDLKNTLNDYD--KELKQSKDKVTAEVDKQNQLQQKLTEAE---------SKILELTQKLDLIAVQQSSDIEKDKQLEKLTVELHAAKQTSAESVAKSETLVKNLKADIENKVKDLRLKEDLIGKL---QEEVKSQKAKVEIAEREKLLLQKEMAMKGKDVRDKNDNSAMGVLGQGDTASEKTTDDKEMIDG----QVSFLNSVIVDMQRKNEQLMARVQALEGGSV----APEPVLFNGRKARAVAPRLFCDICDVFDAHDTEDCPKQAGEPDAPPPPANRRAAPPP-RPYCDICEVFGHATENCDEEETF 1770          

BLAST of putative restin vs. nr
Match: gi|1199381176|ref|XP_021185110.1| (restin homolog isoform X4 [Helicoverpa armigera])

HSP 1 Score: 439.884 bits (1130), Expect = 2.385e-123
Identity = 528/1867 (28.28%), Postives = 897/1867 (48.04%), Query Frame = 0

Query:    8 KVWVGGTKSGKIAFLGETKFAPGEWAGVALDDALGKNDGTVGGVSYFKCAPMHGVFSRASRLSRQPLASGPP----------SCLTTPRKVSKAGRASMSPSESVVDLKSGTPSPTGSTISLVSMAPLKLGDRVIVGNAVTGTKMGTLRFMGPTEFAQGDWGGVELDSAIGKNDGMVGDKRYFECPPNFGLFAPLHKITKSPKARMQRQPSSLTPRKLNRE---RSDLSEASMASMMSTVSRSQVPVK--------------KKSIASIMAPERLKMSLKEKEDRIDQLLMEREQERAQIAKAASQTDVAEGNLSKIIEEYGSYKREQEERALTYRATMEKYEEKIRSLKNKLEDEKRKHDDVEFRLEEETMMRSESDAGNSGELIEKIRSLEDQLAQELKAKADLNGGKSAF---------ETRIADLEGKLGSEEKMRTDWTRDQSQMKIEMDNLAEQLETEHRKNEARGKEVDSLKTRL-VATEDKLKEARTHLDVVKEESGNRVFESEEQIIMLKDEVDTLRKDLDESKGSLEREEKNMAALQESLKEQSTKSIDQISELEKALSDKDNLLLESQHKLDSLQSGSSSVQSTLEEKSRELKENLKLK----QDLKDQLTKAESEIRSLKEEL----DGAKTKV---EGLSNQVGQRDETIKSLEGQKTKIHKQFGVKSKEIDELKSQL-------DKTGSDTQVALQEKSEKVIELSSKLEDAQSSVVALKTDLELEHAKAARAKADVEEAMQELSDVKMAKEDLEKEIRLFKDSSQTSTDEVKRLSGTINSKDLELEMLRGDLSSLTSKLEELKLVIDQSETEHQ--DQLEQIQKD--AKKTTHELE--EKLKKSEATKEKA---EKAKEEAIGELHGKIRQTANDHDDLVKSLRGDLSKKTYDLEGLQSKFSELEKSLLSTSDESSKALTELRQKMVDMEIAAGDQERAMEVLKEEIQTKTATISDLESIKESLEVTRAERDKALGTTKDFGQKLANSQGKLSELAKEKIKLEEAIHAMQSTSVDSDALIGNLNDELKANKAEVERVREELEQKVEQHQKDLDDLAKEQAAKGAKMVKISSAKDKLELDLETTQKDNAVLKENSILAKAQF---DDELTRAKLQADQILEEQKSRERAMKNQMEDLQGELKMRGSTSEETEKALKD------EKSKRMELEEAMEKLRRSHEEEVEELVDVVNELRSKEID--------------LADKTEELELAEKSIKDLNKKLGQHHKELDVFKEKHSKEIDVLNKSLEESKSQIVFLKESMGGNKVEYDLQMDKFKKQIEAQKSKFEDEMDKKTDLIKSMKDDITNKEEELEFAKEELEAKIEEFND---LNTDLEVKEASFDNSFKETDEKH---KDEIEVLVTKIEEARAEVADLSETLAKTRAEHDSKMDEMMSGKKSEVNSLQDQISERDRSILD--LKASLEELEGRVTSLNSSSADEGK-IHEAKVA--KITSDNEDLRLKKKDLESTLKSLQADFDQLSNQNEDLSIKVVEREHQVREYTSQVQTMKVSYEKSQKELTEKAEKSNQDGDALKELEKALDDERIRVD---ELQIALAEAETERDSARSKSKDFQKISTQNVTLDEDNENLKKD--IEELKHKLHFAQDAFESERTELNEVVQKSKVLLQDKVEEFKSQKREFDALVLENQQLNSYRRKVMILEQEKRELESQVVTLTYESRS----------GRKSPSPAKSI-SSDMNDGELKAQVDFLNSVIVDMQRKNDDLKTKLEIFESAGIIDSEAEANFMFNGVSSRSVAPRLFCDICDVFDIHDTEDCPTQAMEISEPVIHTKSGGARGQIREYCDTCEVFGHSTANCNEGETF 1760
            +VWVGGTK G+IA++GET+FAPG+WAG+ LD+ +GKNDG+V GV YF+C    GVFSR +RL+R PL +  P          S    P   ++  RA +SP+ S+   +S   S   ++IS  +   +++GDRVIV ++  G+K GTLR++G T+FA G W GVELD  +GKNDG V  KRYFEC P FGLFAP+ K+++SP     R+P +       R    R   S  S+ S+ ++++ S+V V+              + S   + A   L+  L+EK+  +++L+ ERE ERA++AK + Q D AE  L+++        +++  +A +  + ++   EKI  L   LEDEK+K++D+ FR EEE + R   D     + +E+ +++ +Q  +EL+A+A+L   ++           + R AD+       ++         S+++  +D     L+ +  + ++ GK +    TRL   +E  L EA+T + + + E     FE++  ++       T +  L ESK  LE E++N+  L    KE S   ID  ++L +  +  D+  LE    L+ +   S   +  L ++  +LK  L  K    + L+D   K E+  +SL+EEL    D   +KV   E L ++V Q+ E  K+   +  ++ +    K+KE ++L ++L       +K  S+ +  + E+ +++I+L    E A +       +  ++H+K A      EE  +E+ D     E L KEI   K               T++   +E E L    S  T  ++ELKL+ +++E   Q  ++L Q   D  AK +  ELE  E  ++ E+  EK    +KAK++   E      QT + ++                      +F++L + L     E+ K L EL+ K  ++     +  RA + LK++          L  I+E   V   ER + + +  +  +KL  + GK+ E   + +++   +  +Q+   D+D L+   N  LK N+ E  +V+E+ EQ +E      D + K                 KLE+D++  +++ ++LK +   ++ +     D+LT A+   +  LEE +  E +++  ++D+  +L  + +  E+    L++      E++ +++ EE   +L+   E++   + D+  +L   E+D              LA   EEL  A +S K L +KL     E    K+K+  E  V N S EE+      LKE +G               Q+E  K +    + +KT L     + +     EL   K+  E  + + ND   +N +L+      D+  K+  EK+    ++I  L T++++ + EV++  +T+ + +    +KM + +    SE   L  Q+ + + S L+   K  +E L   + +L +  A++GK + E K A  K+T           +L+  +   + D  QL+N NE        ++    +   Q+Q     Y+K  K      + +  D D  KEL+++ D     VD   +LQ  L EAE         SK  +     ++   + + +++KD  +E+L  +LH A+        +   +V+  K  +++KV++ + ++     L    +++ S + KV I E+EK  L+ ++     + R           G+   +  K+    +M DG    QV FLNSVIVDMQRKN+ L  +++  E   +    A    +FNG  +R+VAPRLFCDICDVFD HDTEDCP QA E   P        A    R YCD CEVFGH+T NC+E ETF
Sbjct:  145 RVWVGGTKPGQIAYIGETQFAPGDWAGIVLDEPIGKNDGSVAGVRYFQCPEKRGVFSRLTRLTRVPLITHAPHDASPISDAGSVFERPPSGTRQRRA-LSPNGSI---RSIVSSKMNASISTTTNGDVRVGDRVIVSSS-RGSKAGTLRYVGVTDFATGVWAGVELDDPLGKNDGSVDGKRYFECSPRFGLFAPVSKVSRSPS---NRKPGACAIHSNGRATPLRRSNSRESLTSLGTSIASSRVGVRLGVTSLGAQRVGGPRASSTPVSAKNALQELLREKQQHLERLMRERELERAEMAKISLQVDRAETALTQL--------KKENTQATSENSKLKAELEKINKL---LEDEKQKNEDLMFRTEEENINR--EDYNKYKDAMEQEKAVREQRIRELEAEAELQTARAEATATALRMHEDQRAADIAAANQQHQEELAAAHTLSSELQKLLDEAYALLKEKENEKDSLGKSMSEELTRLKTDSEKALNEAKTKMAIAQTE-----FETQVSVL-------TAKLQLAESK--LETEKQNLERLN---KENSQIIIDLNTKLTQLQATVDDKTLE----LNKVVGVSKEHEVNLNKEITKLKMELSAKILDLEQLEDSKRKQETVFKSLQEELGRVKDEYNSKVNEYETLLSEVSQQHEKNKT---EMMELQQNLSAKTKEYEKLLAELSTSTNSSEKLVSEYKETIHERDKEIIKLKEDYEQAAA-------NFNIKHSKIA------EEHKKEIDDRNSKIEQLMKEIESHKQ--------------TLDQSKVEFEALN---SQFTLNVDELKLLKEENEKLKQSINELTQANNDLKAKMSAMELEVGEYKRQFESANEKCAELQKAKDKIESEYMNLTGQTTDSNE----------------------QFNKLSQHL----KETEKELQELKDKHREV---VNNHARAEQELKQK----------LFKIQEDFSV---ERGQLVVSVNENIEKLKEADGKIKEFETQTVEVNNRLRNLQT---DNDKLLDE-NSILK-NEIEALKVKEQ-EQNMEH-----DTICK-----------------KLEIDIDRYKEEISILKADGATSEVKLMEKVDQLTEAQNDLNNKLEEARKHEDSLQKILDDMTAQLNNQKTNFEKEISQLQNNLSTVTEETNQLKAEE--NRLKEILEQKQNNVKDLTLKLEMLEVDLKSNVEIVTEKDRQLAQVNEELNKATESKKQLEEKLNGAALETSALKQKY--ESLVANSSAEET-----VLKEQLG---------------QLEVLKKEMSTLVQEKTSLESKYSETLA----ELTTIKKNYEDAVNKINDNVTVNNELKKSVEEKDSILKQQTEKNVIDAEKISKLETELDQLKQEVSN-KDTVIQEKESQLTKMHDALQLGSSETQQLVAQL-QLENSKLNETYKTEVESLNNTLKTLQTQVAEKGKELEELKQASEKVT-----------ELQKLVDKSEQDIKQLTNINE-------AQKMNYEDLNKQLQAQFDEYKKESKRARHDLKNTLNDYD--KELKQSKDKVTAEVDKQNQLQQKLTEAE---------SKILELTQKLDLIAVQQSSDIEKDKQLEKLTVELHAAKQTSAESVAKSETLVKNLKADIENKVKDLRLKEDLIGKL---QEEVKSQKAKVEIAEREKLLLQKEMAMKGKDVRDKNDNSAMGVLGQGDTASEKTTDDKEMIDG----QVSFLNSVIVDMQRKNEQLMARVQALEGGSV----APEPVLFNGRKARAVAPRLFCDICDVFDAHDTEDCPKQAGEPDAPPPPANRRAAPPP-RPYCDICEVFGHATENCDEEETF 1795