BLAST of Pikachurin partial vs. L. salmonis genes
Match: EMLSAG00000007498 (supercontig:LSalAtl2s:LSalAtl2s430:288057:377591:1 gene:EMLSAG00000007498 transcript:EMLSAT00000007498 description:"maker-LSalAtl2s430-augustus-gene-4.9")

HSP 1 Score: 250.366 bits (638), Expect = 6.056e-77
Identity = 140/328 (42.68%), Postives = 187/328 (57.01%), Query Frame = 0

Query:   26 MIQSAIAEHASSVPFGDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQ--------SQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNT------QAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG-GDGFTP--ASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEH 336
            ++     E A  +  G    RS  PI+LH W+T+ +SRTGRLA + V   K +        S++  GAF++LT  QN+YLGGVPTF LVS Y+PIR+   GCIQKL+IN+   +L+  AI+G++V  C HPC+   ++      Q PCG+HG C+P L+ F C+CP G  G +C K                              I G +NN   R K +K N LI W G G  F      DY+ LG+  G L  ++NLG+GE  + +N TRINDG+WHRIR TR E TASL++DNG I T  SPGKL+QLN NG+V++G    L H
Sbjct:  405 LLSRNYVEFAYDLGDGITVVRSASPISLHVWYTVVISRTGRLAEMEVIRGKREDPLKMPVSSRVSNGAFSQLTLEQNLYLGGVPTFDLVSAYLPIRRSFFGCIQKLTINDVEINLVNSAISGVDVGSCDHPCNTPSSSSSNEFPQHPCGVHGNCKPVLDGFKCDCPHGLTGNSCQK------------------------------ITGESNNPLPRFKKSK-NALIFWAGNGRNFKKGIVGDYIALGIYNGRLQLKYNLGSGEASITYNWTRINDGKWHRIRLTRNELTASLRMDNGPIYTTMSPGKLKQLNANGEVYLGXYSFLYH 701          

HSP 2 Score: 65.4698 bits (158), Expect = 8.005e-12
Identity = 73/301 (24.25%), Postives = 130/301 (43.19%), Query Frame = 0

Query:   98 GGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLR-----GAIAGLNVNPCSHPCSPVPN-TQAPCGLHGTCRPNLEDFSCECPLGWVGITC-SKKMNLTKS--SAMIPRFGGRSYLHLNREELAKNILGNTN----NINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGH--------IRTGASPGKLRQLNGNGQVFIGGVE--DLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            GG   F+    Y+ +  G  GCI++L INN   D         +  G+++  C     P  N    PC  +G C P     +      W  I C +  ++L K   S  +P F G SY       L    +G+++     I +  + +  +GLI + G   DG     D++ + +   ++ F ++LG+G  V + + + I+   W+ +  +RT + A ++V  G         + +  S G   QL     +++GGV   DL    LP  R    GCI  ++I  + +I ++  +  G ++
Sbjct:  252 GGAGYFRNYGHYLGVETGFSGCIKRLEINNKVYDFRSKEHGGDSQFGMDITEC-----PEDNCVLIPCQHNGKCVPLESGHNI-----WWAINCGTLSISLRKGIISKNVPSFNGTSY-------LVYTGIGDSSPLWTEIELVFQSSAEDGLIFYNGQRNDG---TDDFIAILLSRNYVEFAYDLGDGITV-VRSASPISLHVWYTVVISRTGRLAEMEVIRGKREDPLKMPVSSRVSNGAFSQLTLEQNLYLGGVPTFDLVSAYLP-IRRSFFGCIQKLTINDV-EINLVNSAISGVDV 529          

BLAST of Pikachurin partial vs. L. salmonis genes
Match: EMLSAG00000003115 (supercontig:LSalAtl2s:LSalAtl2s174:638829:676917:-1 gene:EMLSAG00000003115 transcript:EMLSAT00000003115 description:"maker-LSalAtl2s174-augustus-gene-6.13")

HSP 1 Score: 97.0561 bits (240), Expect = 4.167e-22
Identity = 79/333 (23.72%), Postives = 141/333 (42.34%), Query Frame = 0

Query:   57 HTIKVSRTGRLAFLAVDDQK-----------TQSQMG--------PGAFNELTANQNMYLGGVPTFK------LVSPYIPIRQGLQGCIQKLSINNSPRDLLR-------GAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCEC-PLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREEL-AKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNG--EGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADI 353
            H ++V + G    L VDD+K           TQS           P  F +  +N  +Y+GG+PT+       L  P + +    +G I  +   ++   + +         +    VN C H        Q PC   G C P      C+C  + ++G+ C      TK   +   F    Y+  + ++   + IL   ++I++  K  K +GL+ +TG       +DY+ LG+ +G +     LG G  +  +  +  R ND +WH ++ +R     +L VD+ +     + G    L  + +VF+GG E    LP  + ++ L+GC+  +
Sbjct:  106 HRVRVLKNGAETSLIVDDRKESLLTPLVLTSTQSSASLPPSTIPVPYQFGDPVSNSFLYIGGLPTWYREKFDILTLPTVILEPRFRGEISDIKFKDARTKVFKIQEVLAYKGVRASTVNACDH--------QXPCLNDGICLPTDSGPICDCSKVDFIGLXCE-----TKKQPLEATFRHGDYIXYDXQQRKVEPILSTNDDISLFFKTRKSSGLLFYTGN-----GNDYMNLGIKDGGVSLTVQLGTGKLDTQIKPSTVRFNDNKWHHVKVSRMSSEVTLTVDDLYNERWVTSGSFSVL-ASTKVFVGGSEATYTLPGTKAQNNLVGCLRSV 419          

HSP 2 Score: 92.0485 bits (227), Expect = 2.088e-20
Identity = 78/316 (24.68%), Postives = 146/316 (46.20%), Query Frame = 0

Query:   54 HQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGG-------RSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLE-HLPLP------EFRHGLMGCIADISI 355
            ++WH +KVSR      L VDD   +  +  G+F+ L + +    G   T+ L  P    +  L GC++ +       D L   + GL  N  +   S + + +  C          +D +   PL +     S   N+     M    GG        S+L ++       I G+   I+++ +  + NG++L+ GG+   P  DY    +L+G L+   +LG+G   +  + T +NDG WH +  T ++++  +K+D     +  +PG+  QL+  G +F+GGV+ ++ ++  P        ++G +GC+ D++I
Sbjct:  352 NKWHHVKVSRMSSEVTLTVDDLYNERWVTSGSFSVLASTKVFVGGSEATYTL--PGTKAQNNLVGCLRSVIYT---ADSLILDLLGLAENN-NQLISVIGDVEYEC----------QDIAAVPPLPFKSQEDSSS-NMASIYMMQNNLGGIISFTTQESFL-VSFTSWKSTISGS---ISLKFRTNELNGILLFNGGN---PHKDYFAFEILDGHLYVHLDLGSGPVKIRASRTPLNDGAWHHVELTLSQRSGRIKIDEDS-ESFKTPGEANQLDLVGNLFVGGVDYMDPYMKFPPTLWTVTLKYGFVGCLRDLTI 642          

HSP 3 Score: 67.781 bits (164), Expect = 1.681e-12
Identity = 64/274 (23.36%), Postives = 116/274 (42.34%), Query Frame = 0

Query:   47 SEYPITLHQWHTIKVS---RTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPY---------IPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNT--QAPCGLHGTCRPNLEDFSCECP-LGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDN 305
            S  P+    WH ++++   R+GR+      D+ ++S   PG  N+L    N+++GGV     + PY         + ++ G  GC++ L+IN +  D++  A    +V      C  +P+     PC   G C      F C+C    + G TC         +A    F G   + L+ ++         ++I +R +  + +G++  T  D    + D L + +  G +   F +G+ E   IF    +ND  +H I   R      + VD+
Sbjct:  559 SRTPLNDGAWHHVELTLSQRSGRIKI----DEDSESFKTPGEANQLDLVGNLFVGGV---DYMDPYMKFPPTLWTVTLKYGFVGCLRDLTINGASIDVVSYA-HQQDVGSIRSSCHKMPDQCHDRPCNHGGKCSEGWNRFLCDCSRTSFTGATCGY-------AAATVIFEGSHSISLSYKD---GTTTEADDIILRFRSMEQHGILFTTHHD---TSKDKLDVTMESGRVRLSFQVGHSEKT-IFAGQSLNDDIYHTILLQRRGTKIRVVVDD 810          

BLAST of Pikachurin partial vs. L. salmonis genes
Match: EMLSAG00000003993 (supercontig:LSalAtl2s:LSalAtl2s213:441137:447252:-1 gene:EMLSAG00000003993 transcript:EMLSAT00000003993 description:"maker-LSalAtl2s213-snap-gene-4.21")

HSP 1 Score: 94.3597 bits (233), Expect = 3.709e-21
Identity = 89/372 (23.92%), Postives = 143/372 (38.44%), Query Frame = 0

Query:   51 ITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGA--IAGLNVNPCSH------------PCSPVPNTQAPCGLHGTCRPNL--------------------------EDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGV---EDLEHLPLPEFRHGLMGCIADISI-GRLFDIKMMEESS--DGRNITLA 376
            + + +W++++  R GR  +L  D Q T      G  N L  +Q ++LGG+P   ++      +   +GCI+ L IN     L        G N+  C               CS   N     G    C  N                           E   C+CPLG+ G  CS +  +      +PRF G  ++          +L  T   +IR   T                + DYL +G+ +G +H  +NLG      +   +++ND +WHR+   R  Q   L VD G I      G   +LN    V IGG    ++++ +    +  G  GC+ +++I     D  + ++SS   GRNI + 
Sbjct: 1144 VEIDKWYSVEAGRYGRKGYLRXDGQFTTGSTESG-LNTLDVDQELFLGGIPNSHMLFIDFVSKHYYEGCIRNLHINEKSYPLTSTGNHWKGWNIKDCDGTACGGELCQNGGKCSLSENIHNHRGYECGCPDNYIGEHCEQHRLCINACENDGICSIGDDEIIHCDCPLGYHGSFCSDEEEI-----RVPRFYGDGFISF--------VLSETK--SIRHDQT----------------SDDYLGVGLKDGMVHLVWNLGWFSRTELTVPSKVNDDKWHRVLIDRIRQELKLDVD-GKIYQSRVTGSYHELNTVNVVLIGGSSEKDNVQDMTRGHYSMGFNGCMRNVTIQSSSMDKALKQKSSGIQGRNIDIC 1482          

HSP 2 Score: 80.4925 bits (197), Expect = 1.354e-16
Identity = 89/345 (25.80%), Postives = 141/345 (40.87%), Query Frame = 0

Query:   57 HTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNEL-TANQNMYLGGVPT--FKLVSPYIPIRQGLQGCIQKLSINNS----------PRDLLRGAIAGLNVNPCSHP-CSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKK------------------MNLTKSSAMIPRFGGR-------SYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRIND-----GRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEF--RHGLMGCIADISI 355
            H ++  R GR  +L VD+    + + PG    L   +   Y+GG     F L+    P+ +G  GC+  ++  +           P  +    I G NV  C +  CS      +PC   GTC      F CEC  G  GITCS+                   + L  S   I  FG         SY+   +  L   I  NT +I+I ++     G I+  G        D+L L ++ G + F F+LG   G  + N   I        +W+ + A R  +   L+ D G   TG++   L  L+ + ++F+GG+ +  H+   +F  +H   GCI ++ I
Sbjct:  884 HAVRFGRNGRRGWLQVDNFPNITGLSPGKREFLNVTSMYYYIGGHENYNFSLLPHDFPVHRGFSGCMFDMAYRSYVTGQLTPLALPSKIGSSPIRGRNVEQCHYDICS-----SSPCSNGGTCIGXGASFLCECTDGRYGITCSEDTSACTTGMHKCAVSSKCTVTLKGSYECICPFGKNPLFTGHGSYM---QTHLKSAIRFNT-HIHIEIRPQTLEGHIIHLGQSRNGRHQDFLSLLLVNGSVVFSFSLGGPIGGNVGNTVTIRTCCVEIDKWYSVEAGRYGRKGYLRXD-GQFTTGSTESGLNTLDVDQELFLGGIPN-SHMLFIDFVSKHYYEGCIRNLHI 1217          

HSP 3 Score: 53.1434 bits (126), Expect = 7.710e-8
Identity = 54/233 (23.18%), Postives = 103/233 (44.21%), Query Frame = 0

Query:  155 TQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRL--KVTKPNGLILWTGGD-GFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQ-TASLKVDNGHIRTGASPGKLRQLNGNGQV----------FIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            T   C   GTC   L    C CPL + G+ C +++ +       P F G S L     +L+KN +    N +++L  + ++  GLIL+TG D G+   S Y+  G+L       F +  G  +++F++ R      HR+     +  + ++ + +    T     + R ++G+ Q+          ++G V   + L       G  GC+  + +    ++ +  ++ DGR+I
Sbjct:  470 TSNVCSNGGTCLTTLSGVKCICPLQYHGVQCEEEVFIET-----PGFVGHSGL---VHKLSKNNIVKDRNFHVQLSFRTSQEEGLILFTGEDVGYLLVSGYIKDGIL------TFKISCGHQIILFSDPR------HRVDXGYLQXLSINITIFDTICSTSIQLNETRTMSGDQQIDSLPQIPDMLYMGLVPHKKELGNGIINVGFQGCMKHLQLNS-NELNLYRDAVDGRDI 681          

BLAST of Pikachurin partial vs. L. salmonis genes
Match: EMLSAG00000012352 (supercontig:LSalAtl2s:LSalAtl2s903:176857:182009:1 gene:EMLSAG00000012352 transcript:EMLSAT00000012352 description:"maker-LSalAtl2s903-augustus-gene-1.45")

HSP 1 Score: 76.2554 bits (186), Expect = 1.948e-16
Identity = 47/145 (32.41%), Postives = 75/145 (51.72%), Query Frame = 0

Query:  220 GNTNN-INIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVL-IFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLP-----EFRHGLMGCIADISIGR 357
            G TNN   + L+  + NGL++W    G +   D+L + +++G+    + LG  +  L I ++ RIND +WHR++  R  +T  ++VD      G S      LN NG+V++GG E     PLP     ++  G  GCI  + I R
Sbjct:   46 GETNNKFTVHLRTIEKNGLLIWIN-QGTSLKGDFLAVAIVDGYPELSYKLGKKQNTLRIKSKKRINDDKWHRLKVIRRRRTGLIQVDKLKPIRGKSGTGASILNTNGKVWLGGKE-----PLPPGLPHQYYKGFKGCIRKVKIFR 184          

BLAST of Pikachurin partial vs. L. salmonis genes
Match: EMLSAG00000005174 (supercontig:LSalAtl2s:LSalAtl2s273:334165:338227:-1 gene:EMLSAG00000005174 transcript:EMLSAT00000005174 description:"maker-LSalAtl2s273-augustus-gene-3.9")

HSP 1 Score: 71.633 bits (174), Expect = 1.029e-13
Identity = 71/347 (20.46%), Postives = 139/347 (40.06%), Query Frame = 0

Query:   43 LSARSEYPITLHQWHTIKVSRTGRLAFLAVD-DQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGA------IAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLG-WVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVL-EGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLK--VDNGHIRTGASPGKLR-----QLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            ++  + Y +   +WH++ + R  + A + VD  +K Q++   G    +     +Y+G    F           G  GC++ L +N    DL+  A      + G+ +  C+  C+       PC   G C    + F+C+C    + G  C+ ++ +   S  + ++  +        E           I++    T P+G ++    D    + +YL L V   G +   F+ G     +++ E     G++H +R  R +Q   +K  VDN   +  +    L+     Q N    ++IG  E +        R G +GCI+ +    +  +K++ +     NI
Sbjct:  867 VTVETSYRLDDDKWHSVLIERNRKEAMVVVDGARKGQAKEPSGPVRSMRLTSKLYVGSTLDFA---------DGFVGCMRALILNGVSVDLIGEATRNPWGLYGIGIG-CTGKCN-----SEPCLNGGECVEGYDHFTCDCRWTPFKGPICADEIGVNMRSDYMIKYDFKGNYKSTIAE----------KIHVGFTTTDPSGFLIGAYSD---ISKEYLTLMVSNSGHIRLVFDFGFERQEIVYTEQNFLTGQYHDVRIERFDQGRQIKLTVDNYMPKIVSFEDSLKSSADAQFNNIRYLYIGRNESM--------REGFVGCISRVEFDEIIPLKLLFQEDPLSNI 1177          

BLAST of Pikachurin partial vs. L. salmonis genes
Match: EMLSAG00000000936 (supercontig:LSalAtl2s:LSalAtl2s1165:50208:68601:-1 gene:EMLSAG00000000936 transcript:EMLSAT00000000936 description:"maker-LSalAtl2s1165-augustus-gene-0.27")

HSP 1 Score: 67.781 bits (164), Expect = 2.354e-12
Identity = 70/301 (23.26%), Postives = 118/301 (39.20%), Query Frame = 0

Query:   73 DDQKTQSQMGPGAFNE-LTANQNMYLGGVPTFKLVS-----PYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAP---CGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGN---GEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASP-----GKLRQLNGNGQVFIGGVEDLEHLPLPE-FRHGLMGCIADISI 355
            DD   Q +     FNE L  N  + +GG+   +         + P+ +   GCI+ +  N+   DL    +   +  P   P     N   P   CG HG C  +L+  +C+C  GW G  CS +   T  S   P+   +  L    +  A         I +R +  + +G +            +Y +L + E  L FR+NL +    E  +  +   ++DG+WH ++  R    A L +D G  R           +L  ++    V+ GG  +   +   E +     GC+ DI +
Sbjct: 2395 DDSSCQVKGDVPPFNEYLNVNTPLQIGGMAAXQFDPLHYKWSHTPLGKPFDGCIRNIIHNSKLYDLAEPGLFRYS-KPGCIPVEKACNDNYPGSRCGEHGHCVGSLKHPTCKCNPGWTGQFCSHE---TMPSTFDPQSYVKYALSFEPDSFA-------TEIQLRFRTREEHGELFRVSDQH---NREYGILEIQESKLRFRYNLNSLRTEEKEIWLSSIYVDDGQWHTVKVARYGSAAILTLDGGESRRYNDSFIFDGHQLMTVDKQEGVYAGGKAEYTGVRTFEVYSDYAKGCMDDIRL 2681          

BLAST of Pikachurin partial vs. L. salmonis genes
Match: EMLSAG00000003184 (supercontig:LSalAtl2s:LSalAtl2s176:575372:647076:1 gene:EMLSAG00000003184 transcript:EMLSAT00000003184 description:"augustus_masked-LSalAtl2s176-processed-gene-6.1")

HSP 1 Score: 62.003 bits (149), Expect = 1.237e-10
Identity = 52/210 (24.76%), Postives = 93/210 (44.29%), Query Frame = 0

Query:  153 PNTQAPCGLHGTCR--PNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGV-EDLEHLPLPEFR----HGLMGCIADISI 355
            P  Q  C  HG C   P   ++ C+C  G+ G  C    +L+            SY+ +     A N+    N I I     + NG++L+TG +       +L + +  G +   +++GN     +F+   ++DG++HR+     ++  +L+VD G  R+  + G    L     +FI GV ED+ H  L ++         GCI ++ +
Sbjct:  495 PCQQHDCK-HGICFQPPGSNNYICKCAPGYSGKRCEYLTSLS-------FIHNTSYVEME----ALNVKPKAN-ITIIFATEQQNGVLLYTGDN------QHLAVELFRGRIRVSYDVGNYPVSTMFSYELLSDGKYHRVELLAIKKNFTLRVDGGIARSIVNEGNNEYLEIRNSLFIAGVPEDVGHKSLKQWHLRNSTSFKGCIKEVYV 685          

BLAST of Pikachurin partial vs. L. salmonis genes
Match: EMLSAG00000006314 (supercontig:LSalAtl2s:LSalAtl2s343:642:15163:-1 gene:EMLSAG00000006314 transcript:EMLSAT00000006314 description:"maker-LSalAtl2s343-augustus-gene-0.12")

HSP 1 Score: 61.2326 bits (147), Expect = 2.713e-10
Identity = 40/148 (27.03%), Postives = 67/148 (45.27%), Query Frame = 0

Query:  158 PCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDN 305
            PC   G C     D+ C CP  + G  C            +  FG   +  L+       +  NTN+I+I    TK N L+++  G+     SD+L L ++ G   F +        ++    +IN+GRW+++ ATR  + ASL +++
Sbjct: 3808 PCLNGGVCISQKPDYRCRCPDNFYGNRCE-----------LSTFG---FDELSFAAFPP-LDSNTNDISITFATTKANSLLVYNHGEVSGGRSDFLALELVRGRPKFSWGAARTAVTILELPRKINNGRWYKVTATRNNRVASLSIED 3940          

BLAST of Pikachurin partial vs. L. salmonis genes
Match: EMLSAG00000001472 (supercontig:LSalAtl2s:LSalAtl2s124:357996:426231:-1 gene:EMLSAG00000001472 transcript:EMLSAT00000001472 description:"maker-LSalAtl2s124-augustus-gene-6.17")

HSP 1 Score: 59.3066 bits (142), Expect = 9.394e-10
Identity = 42/137 (30.66%), Postives = 69/137 (50.36%), Query Frame = 0

Query:  224 NINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETR-INDGRWHRIRATRTEQ-TASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPE---FRHGLMGCIADISI 355
            ++  RL+  + +G+I++  G G     D++ + +  G +H+  NLG G   L  N  R IND +WH +   R  Q   +L+VD  H  +  S G    L+ +G +F+GGV    +  LP      +G  GC+A I +
Sbjct:  508 DLYFRLRTLETDGIIMFNAGRG----QDFIAVELSNGHIHYVLNLGYGVINLRDNSPRKINDNKWHTVSIGRPSQFKHTLRVDTYH-SSANSKGNESNLDLDGILFLGGVRKNMYSKLPSEIISTYGYQGCLACIEL 639          

HSP 2 Score: 51.2174 bits (121), Expect = 3.543e-7
Identity = 25/86 (29.07%), Postives = 49/86 (56.98%), Query Frame = 0

Query:  249 ASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQ----LNGNGQVFIGG 330
            ++D+L + + +G++H  +NLG  + ++      +NDG +H I+ TR+E  ++L++D+ H      P  LR+     N   Q+ +GG
Sbjct:  793 SNDFLEMQIEDGYIHILYNLGTEDIMISDKNILVNDGNYHVIKFTRSEANSTLQIDD-HPTQFKYPIALRRQLTVFNSQSQIHVGG 877          

HSP 3 Score: 50.0618 bits (118), Expect = 8.699e-7
Identity = 32/131 (24.43%), Postives = 60/131 (45.80%), Query Frame = 0

Query:  251 DYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLP-------EFRHGLMGCIADISI-GRLFDIKMMEESSDGRNI 373
            D   + +LEG+L+   NLG     +  +  R++DG WH+I      ++ ++KV N   +  A PG+   L     +++G ++ L+    P           G +GC+ D+ + G   D+    +  D  +I
Sbjct:   97 DVFAIEILEGYLYLHLNLGPEALKIRASNQRLDDGTWHKIEIFLRSKSGTIKV-NEDFQEFAIPGESNGLELLNSLYVGNIDHLDSKITPPPSIWSASLNKGFVGCLRDLEMNGNHIDLHHFVKHQDSGSI 226          

BLAST of Pikachurin partial vs. L. salmonis genes
Match: EMLSAG00000012892 (supercontig:LSalAtl2s:LSalAtl2s981:135679:153834:-1 gene:EMLSAG00000012892 transcript:EMLSAT00000012892 description:"maker-LSalAtl2s981-augustus-gene-1.59")

HSP 1 Score: 53.5286 bits (127), Expect = 6.557e-8
Identity = 78/308 (25.32%), Postives = 120/308 (38.96%), Query Frame = 0

Query:  117 QGCIQKLSINNSPRDLLRGAIAGLNVNPCSHP-CS-PVPNTQA------------PCGLHGTCRPN-LEDFSCECPL---GWVGITCSKKMNLTKSSAMIPRFGGRSY-LHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG---GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKV-DNGHIRTGASPGK--------LRQ-----LNGNGQVFIGGV--------EDLEHLPLPE----FRHGLMGCIADISI--GRLFDIKMMEESSDGRNIT 374
            +GC   L I+ SP  +     + + V     P CS  +P+ Q             PC   G C  N L  + CECP    G  G  C +         +   F G  +  H +      + L      ++     + +G +L+ G   G      +DYL L +  G L    N G+G   L   + RI+DG+ H +    T  +A +++ DN    TG S  K        +R+     LN NG + IGGV        + L+ L L      +  G  GCI +++   G    +  + + SDG N T
Sbjct: 1030 EGCTNYLDIDESPVTVYTNTSSFVGVGAWVKPFCSCSLPDAQKSVSKYKNPCDHNPCLNGGNCISNGLASYKCECPSSNNGQFGXNCER---------LAASFNGEGWSWHPSLSACGDSAL------SLSFNTDRDSGTLLYVGPTPGSVVENITDYLALEIEAGRLKMYLNYGSGTKTLSLGQ-RIDDGKTHELVMKWTNTSARMELDDNSCTSTGRSDKKNQCFIQISMRKSKNSFLNVNGPLQIGGVSFGTNGFQDLLQRLSLKSSEMPYGDGFAGCIRNLTYRAGSRTVLYDLGDPSDGDNYT 1321          

BLAST of Pikachurin partial vs. SwissProt
Match: gi|158705944|sp|Q63HQ2.2|EGFLA_HUMAN (RecName: Full=Pikachurin; AltName: Full=Agrin-like protein; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor)

HSP 1 Score: 250.751 bits (639), Expect = 7.906e-74
Identity = 123/333 (36.94%), Postives = 197/333 (59.16%), Query Frame = 0

Query:   46 RSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKK-----MNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            RSE P+TL  WH ++VSRT +   L VD QK    M  G F ++  N ++++GGVP +  V     + +   G IQK+ +N+    +     +G+NV   +HPC      +APC   G+CRP  E + C+CPLG+ G+ C K+     +N    +  IP+F GRSYL  +  ++ K + G+ +N+ +R K T  +GL+LW G     P SD++ LG+ +G L F +NLG+G   ++ N +  NDGRWHR++A R  Q+  + VD+   RTG SPG +RQLN NG +++GG++++      ++  GL+GCI+  ++   + I ++E++ DG+NI
Sbjct:  685 RSEDPLTLGNWHELRVSRTAKNGILQVDKQKIVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGVLKPFSGSIQKIILNDRTIHVKHDFTSGVNVENAAHPC-----VRAPCAHGGSCRPRKEGYDCDCPLGFEGLHCQKECGNYCLNTIIEAIEIPQFIGRSYLTYDNPDILKRVSGSRSNVFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGS-FNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNI 1011          

HSP 2 Score: 120.553 bits (301), Expect = 1.909e-28
Identity = 87/298 (29.19%), Postives = 141/298 (47.32%), Query Frame = 0

Query:   47 SEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDL----LRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRP-NLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRF--GGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL 337
            SE  I L  WHT+ + R G    L +++    +    G ++++T    +YLGG P+   +       +G QGC+Q L++N    D+    L  A++G +V  CS         +A C   GTC     + + C CPLG+ G  C     LT     IP+F    RSY         ++ L +     I  +    +G++L++   G   + D+L + +  G + FRF+ G+G GVL  +E  +  G WH +R +RT +   L+VD   I  G + G   Q+  N  +FIGGV + + +
Sbjct:  462 SETKIKLGGWHTVMLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGRRIDMRPWPLGKALSGADVGECSSGIC----DEASCIHGGTCTAIKADSYICLCPLGFKGRHCEDAFTLT-----IPQFRESLRSYAATPWPLEPQHYL-SFMEFEITFRPDSGDGVLLYSYDTG---SKDFLSINLAGGHVEFRFDCGSGTGVL-RSEDPLTLGNWHELRVSRTAKNGILQVDKQKIVEGMAEGGFTQIKCNTDIFIGGVPNYDDV 745          

HSP 3 Score: 71.2478 bits (173), Expect = 3.654e-12
Identity = 52/191 (27.23%), Postives = 90/191 (47.12%), Query Frame = 0

Query:  174 CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPE-FRHGLMGCIADISI-GRLFDIK 362
            C+C LG  G +CS+ + +       P+F G SY+     E  KN       I +  +    +GL+L+ G +      D++ L ++   L FRFN G G  +++ +ET+I  G WH +   R      L+++NG   TG S G+  ++     +++GG      L        G  GC+  +++ GR  D++
Sbjct:  369 CQCTLGKGGESCSEDIVI-----QYPQFFGHSYVTF---EPLKNSY-QAFQITLEFRAEAEDGLLLYCGENEHG-RGDFMSLAIIRRSLQFRFNCGTGVAIIV-SETKIKLGGWHTVMLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGRRIDMR 548          

BLAST of Pikachurin partial vs. SwissProt
Match: gi|81908066|sp|Q4VBE4.1|EGFLA_MOUSE (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; AltName: Full=Nectican; Flags: Precursor)

HSP 1 Score: 248.054 bits (632), Expect = 6.490e-73
Identity = 124/333 (37.24%), Postives = 194/333 (58.26%), Query Frame = 0

Query:   46 RSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKK-----MNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            RSE P+TL QWH ++VSRT +   L VD QK    M  G F ++  N ++++GGVP +  V     I     G IQK+ +N+    +     +G+NV   +HPC       APC   G+CRP  E + C+CPLG+ G+ C K+     +N    +  IP+F GRSYL  +   + K + G+ +N  +R K T  +GL+LW G     P SD++ LG+ +G L F +NLG+G   ++ N +  +DGRWHR++A R  Q+  + VD+   RTG SPG +RQLN NG +++GG++++      ++  GL+GCI+  ++   + I ++E++ DG+NI
Sbjct:  685 RSEAPLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIHVKHDFTSGVNVENAAHPC-----VGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNHCLNTIIEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLGSGVASIMVNGS-FSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGLMRQLNINGALYVGGMKEIALHTNRQYLRGLVGCISHFTLSTDYHISLVEDAVDGKNI 1011          

HSP 2 Score: 123.635 bits (309), Expect = 1.642e-29
Identity = 88/298 (29.53%), Postives = 141/298 (47.32%), Query Frame = 0

Query:   47 SEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDL----LRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRP-NLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRF--GGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL 337
            SE  I L  WHT+ + R G    L +++    +    G ++++T    +YLGG P+   +       +G QGC+Q LS+N    D+    L  A+ G +V  CS         +A C   GTC     + + C CPLG+ G  C     LT     IP+F    RSY         ++ L  T    I  +    +G++L++   G   + D+L + +  G + FRF+ G+G GVL  +E  +  G+WH +R +RT +   L+VD   +  G + G   Q+  N  +FIGGV + + +
Sbjct:  462 SETKIKLGAWHTVTLYRDGLNGMLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMRPWPLGKALNGADVGECSSGIC----DEASCIHGGTCAAIKADSYICLCPLGFRGRHCEDAFALT-----IPQFRESLRSYAATPWPLEPQHYLSFT-EFEITFRPDSGDGVLLYSYDTG---SKDFLSINMAAGHVEFRFDCGSGTGVLR-SEAPLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDV 745          

HSP 3 Score: 72.4034 bits (176), Expect = 1.850e-12
Identity = 53/191 (27.75%), Postives = 89/191 (46.60%), Query Frame = 0

Query:  174 CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPE-FRHGLMGCIADISI-GRLFDIK 362
            C C LG  G  CS+ + +       P+F G SY+     E  KN       + +  +    +GL+L+ G +      D++ L ++   LHFRFN G G  ++I +ET+I  G WH +   R      L+++NG   TG S G+  ++     +++GG      L        G  GC+  +S+ G+  D++
Sbjct:  369 CHCNLGKGGEACSEDIFI-----QYPQFFGHSYVTF---EPLKNSY-QAFQVTLEFRAEAEDGLLLYCG-ESEHGRGDFMSLALIRRSLHFRFNCGTGIAIII-SETKIKLGAWHTVTLYRDGLNGMLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMR 548          

BLAST of Pikachurin partial vs. SwissProt
Match: gi|221271989|sp|B4F785.1|EGFLA_RAT (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor)

HSP 1 Score: 245.358 bits (625), Expect = 5.928e-72
Identity = 122/328 (37.20%), Postives = 191/328 (58.23%), Query Frame = 0

Query:   46 RSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            RSE  +TL QWH ++VSRT +   L VD QK    M  G F ++  N ++++GGVP +  V     I     G IQK+ +N+    +     +G+NV   +HPC       APC   G+CRP  E + C+CPLG+ G+ C K +     +  IP+F GRSYL  +   + K + G+ +N  +R K T  +GL+LW G     P SD++ LG+ +G L F +NLG+G   ++ N +  +DGRWHR++A R  Q+  + VD+   RTG SPG +RQLN NG +++GG++++      ++  GL+GCI+  ++   + I ++E++ DG+NI
Sbjct:  681 RSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIHVRHDFTSGVNVENAAHPC-----VGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKAIT---EAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGS-FSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALRTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNI 999          

HSP 2 Score: 120.939 bits (302), Expect = 1.532e-28
Identity = 86/298 (28.86%), Postives = 141/298 (47.32%), Query Frame = 0

Query:   47 SEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDL----LRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRP-NLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRF--GGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL 337
            SE  I L  WH++ + R G    L +++    +    G ++++T    +YLGG P+   +       +G QGC+Q L++N    D+    L  A+ G +V  CS         +A C   GTC     + + C CPLG+ G  C     LT     IP+F    RSY         ++ L  T    I  +    +G++L++     T + D+L + +  G + FRF+ G+G GVL   +T +  G+WH +R +RT +   L+VD   +  G + G   Q+  N  +FIGGV + + +
Sbjct:  458 SETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMRPWPLGKALNGADVGECSSGIC----DEASCINGGTCAAIKADSYICLCPLGFRGRHCEDAFTLT-----IPQFRESLRSYAATPWPLEPQHYLSFT-EFEITFRPDSGDGVLLYSYD---TSSKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDT-LTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDV 741          

HSP 3 Score: 72.0182 bits (175), Expect = 2.455e-12
Identity = 53/191 (27.75%), Postives = 89/191 (46.60%), Query Frame = 0

Query:  174 CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPE-FRHGLMGCIADISI-GRLFDIK 362
            C C LG  G  CS+ + +       P+F G SY+     E  KN       I +  +    +GL+L+ G +      D++ L ++   LHFRFN G G  ++I +ET+I  G WH +   R      L+++NG   TG S G+  ++     +++GG      L        G  GC+  +++ G+  D++
Sbjct:  365 CHCNLGKGGEACSEDIFI-----QYPQFFGHSYVTF---EPLKNSY-QAFQITLEFRAEAEDGLLLYCG-ESEHGRGDFMSLALIRRSLHFRFNCGTGMAIII-SETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMR 544          

BLAST of Pikachurin partial vs. SwissProt
Match: gi|158705693|sp|A3KN33.1|EGFLA_BOVIN (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor)

HSP 1 Score: 244.202 bits (622), Expect = 1.491e-71
Identity = 122/328 (37.20%), Postives = 191/328 (58.23%), Query Frame = 0

Query:   46 RSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            RSE P+TL  WH + VSRT +   L VD QK    M  G F ++  N ++++GGVP +  V     I +   G IQK+ +N+    +      G+NV   +HPC       +PC   G+CRP  E + C+CPLG+ G+ C K +     +  IP+F GRSYL  +  ++ K + G+ +N  +R K T  +GL+LW G     P SD++ LG+ +G L F +NLG+G   ++ N +  NDGRWHR++A R  Q+  + VD+   RTG SPG +RQLN NG +++GG++++      ++  GL+GCI+  ++   + I ++E++ DG+NI
Sbjct:  694 RSEEPLTLGHWHELCVSRTAKNGILQVDKQKAVEGMAEGGFTQIKCNSDIFIGGVPNYDDVKKNSGILKPFSGSIQKIILNDRTIHVKHDFTWGVNVENAAHPC-----VGSPCAHGGSCRPRKEGYECDCPLGFEGLHCQKAIT---EAIEIPQFIGRSYLTYDNPDILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGS-FNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNI 1012          

HSP 2 Score: 118.627 bits (296), Expect = 9.625e-28
Identity = 86/298 (28.86%), Postives = 140/298 (46.98%), Query Frame = 0

Query:   47 SEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDL----LRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLED-FSCECPLGWVGITCSKKMNLTKSSAMIPRFGG--RSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL 337
            SE  I L  WHT+ + R G    L +++    +    G ++++T    +YLGG P+   +       +G QGC+Q L++N    DL    L  A++G +V  CS         +A C   GTC  +  D + C CPLG+ G  C     LT     IP+F    RSY         ++ L +     +  +    +G++L++   G   + D+L + +  G + FRF+ G+G GVL  +E  +  G WH +  +RT +   L+VD      G + G   Q+  N  +FIGGV + + +
Sbjct:  471 SETKIKLGGWHTVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQALTVNGKRLDLRPWPLGKALSGADVGECSSGIC----DEASCINGGTCMASKADSYICLCPLGFRGRHCEDAFTLT-----IPQFKESLRSYAATPWPLEPRHYL-SFMEFEVTFRPDSEDGVLLYSYDTG---SKDFLSINMAGGHVEFRFDCGSGTGVLR-SEEPLTLGHWHELCVSRTAKNGILQVDKQKAVEGMAEGGFTQIKCNSDIFIGGVPNYDDV 754          

HSP 3 Score: 69.707 bits (169), Expect = 1.189e-11
Identity = 52/191 (27.23%), Postives = 89/191 (46.60%), Query Frame = 0

Query:  174 CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPE-FRHGLMGCIADISI-GRLFDIK 362
            C C LG  G +CS+ + +       P+F G SY+     E  KN       I +  +    +GL+L+ G +      D++ L V+   L FRFN G G  +++ +ET+I  G WH +   R      L+++NG   TG S G+  ++     +++GG      L        G  GC+  +++ G+  D++
Sbjct:  378 CHCNLGKGGESCSEDIVI-----QYPQFFGHSYVTF---EPLKNSY-QAFQITLEFRAEAEDGLLLYCGENEHG-RGDFMSLAVIRRSLQFRFNCGTGVAIIV-SETKIKLGGWHTVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQALTVNGKRLDLR 557          

BLAST of Pikachurin partial vs. SwissProt
Match: gi|2494281|sp|Q90404.1|AGRIN_DIPOM (RecName: Full=Agrin)

HSP 1 Score: 145.206 bits (365), Expect = 7.642e-37
Identity = 97/333 (29.13%), Postives = 162/333 (48.65%), Query Frame = 0

Query:   46 RSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGA----FNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSP---RDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSS-AMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDL---EHLPLPEFRHGLMGCIADISIGRLFDIKMMEES 367
            RS+ PI L+ W+ + V R GR   + ++  +  S   P +       L   +  Y+GG P F   +    I  G  G IQKLS+ + P   ++ +R A+   N     H C+    T+ PC   G C P L ++ C C  G+ G  C K +    +S +    F GR+++  +            N   + +K     GLILW+G       SDY+ L V++GF+   ++LG+ + V + +   +N  +W RI+A R     +L+V N    TG+SP    QL+ +G +++GG+E L     LP   +  G +GCI D+ I R  +++++E++
Sbjct:  993 RSKAPIPLNVWNVVTVERNGRKGLMKINKDELVSGESPKSRKAPHTALNLKEAFYVGGAPDFNKFARAAGIISGFTGAIQKLSLKSIPLLKKENIRNAMEISNFR--WHACT---KTRNPCQNGGVCSPRLREYDCMCQRGFSGPQCEKALEEKSASGSESVAFNGRTFIEYHNTVTRSEKAVQVNYFEMSIKTEATKGLILWSGK--IAEKSDYIALAVVDGFVQMTYDLGS-KPVTLRSTIPVNTNQWVRIKANRIHGYGTLQVGNEAPVTGSSPFAATQLDTDGALWLGGIEKLAPGNRLP-KAYSTGFIGCIKDVVIDRQ-ELQLVEDA 1315          

HSP 2 Score: 97.0561 bits (240), Expect = 1.542e-20
Identity = 86/331 (25.98%), Postives = 136/331 (41.09%), Query Frame = 0

Query:   56 WHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPT--FKLVSPYIPIRQGLQGCIQKLSINNSPRDLLR---GAIAGLNVNPC-SHPCSPVP-NTQAPCGL----------------------HGTCRPNLEDFS------------CECPLGWVGITCSK--KMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLR----QLNGNGQVFIGGVEDLEHL 337
            WH +  +R  R   L+VD +       P   + L  + +++LGG P     LV+      +GLQGCI+ L +NN   DL       + G  V  C ++PCSP P   +  C +                      H  C PN    S            CECP+G  G  C +  +    +  A IP F G SYL +N      + L    ++ +      PNG+I + G   DG     D++ L + +G+L F+++LG G  VL  ++  I    W+ +   R  +   +K++   + +G SP   +     LN     ++GG  D    
Sbjct:  731 WHHVVGNRNRRSGMLSVDGEPHLIGESPPGTDGLNLDTDLFLGGTPEDEMTLVTERTTATKGLQGCIRLLDVNNLIYDLQERSNDVLYGSGVGECGNNPCSPNPCKNRGKCHMKEAEMFHCESVGEFSGPTCADKHNPCDPNPCHQSANCMVLPEGGSKCECPMGREGELCERVSEAEQDQGKAFIPEFNGLSYLEMNGIHTFVSDLLQKLSMEVIFLAKDPNGMIFYNGQKTDG---RGDFVSLNLRDGYLEFKYDLGKGAAVL-RSKAPIPLNVWNVVTVERNGRKGLMKINKDELVSGESPKSRKAPHTALNLKEAFYVGGAPDFNKF 1057          

HSP 3 Score: 80.4925 bits (197), Expect = 4.916e-15
Identity = 67/226 (29.65%), Postives = 98/226 (43.36%), Query Frame = 0

Query:  153 PNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLIL--WT-GGDG---FTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPE---FRHGLMGCIADISIGRLFDIKMMEESSD 369
            P    PC   GTC  +   ++C CP G  G  C + +        IP FGGRSYL     +    +      I++  + +  +GL L  WT  G G   + P+         EG++  RFN G  +GV I ++T I  G WH +   R  ++  L VD      G SP     LN +  +F+GG  + E   + E      GL GCI  + +  L    + E S+D
Sbjct:  611 PCDSQPCLHGGTCEDDGVSYTCSCPAGRGGAVCERTIVY-----FIPEFGGRSYLAFKTMKAYYTV-----RISMEFRASNLDGLPLVQWTEKGKGLHFYRPS---------EGYVELRFNHGVWDGV-ITSKTLIKPGNWHHVVGNRNRRSGMLSVDGEPHLIGESPPGTDGLNLDTDLFLGGTPEDEMTLVTERTTATKGLQGCIRLLDVNNLI-YDLQERSND 815          

BLAST of Pikachurin partial vs. SwissProt
Match: gi|460018313|sp|P31696.3|AGRIN_CHICK (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22; Flags: Precursor)

HSP 1 Score: 142.895 bits (359), Expect = 5.367e-36
Identity = 102/353 (28.90%), Postives = 172/353 (48.73%), Query Frame = 0

Query:   46 RSEYPITLHQWHTIKVSRTGRLAFLAVDD------QKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSP---RDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKM-NLTKSSAMIPRFGGRSYL---------HLNREELAKNILG----------NTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLE--HLPLPEFRHGLMGCIADISIGRLFDIKMMEES 367
            RS+ P+ L+ W ++ + R+GR   + +++      +  +S+  P AF  L   +  Y+GG P F  ++    I     G +Q++SI   P      +R A+        +HPC+  PN   PC   GTC P LE + C C  G+ G  C K +       A    F GR+Y+         HL+ E  A + L            +N+  + +K     GLILW+G  G    SDY+ L +++GF+   ++LG+ + V++ +   IN   W  I+A R ++  SL+V N    TG+SP    QL+ +G +++GG+E L   H     +  G +GCI D+ + R  ++ ++E++
Sbjct: 1728 RSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAF--LNLKEPFYVGGAPDFSKLARAAAISTSFYGAVQRISIKGVPLLKEQHIRSAVEISTFR--AHPCTQKPN---PCQNGGTCSPRLESYECACQRGFSGAHCEKVIIEKAAGDAEAIAFDGRTYMEYHNAVTKSHLSNEIPAPDALDYPAEPSEKALQSNHFELSIKTEATQGLILWSG-KGLE-RSDYIALAIVDGFVQMMYDLGS-KPVVLRSTVPINTNHWTHIKAYRVQREGSLQVGNEAPITGSSPLGATQLDTDGALWLGGMERLSVAHKLPKAYSTGFIGCIRDVIVDRQ-ELHLVEDA 2069          

HSP 2 Score: 100.138 bits (248), Expect = 1.468e-21
Identity = 63/209 (30.14%), Postives = 101/209 (48.33%), Query Frame = 0

Query:  153 PNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDI 361
            P    PC   GTC  +  +F+C CP G  G  C K +        IP FGG+SYL     +    +      I +  + T+ +GL+L+ G +      D++ L ++ GF+  RFN G+G GV I ++ R+  G+WH++   R  ++  L VD  H+ +G SP     LN +  +F+GG  + +   + E     +G    I   RL D+
Sbjct: 1350 PCDSHPCLHGGTCEDDGREFTCRCPAGKGGAVCEKPIRY-----FIPSFGGKSYLAFKMMKAYHTV-----RIAMEFRATELSGLLLYNGQNR---GKDFISLALVGGFVELRFNTGSGTGV-ITSKVRVEPGKWHQLVVNRNRRSGMLAVDGEHV-SGESPTGTDGLNLDTDLFVGGAPEDQMAVVAERTAATVGLKGSI---RLLDV 1540          

HSP 3 Score: 88.1965 bits (217), Expect = 1.517e-17
Identity = 85/336 (25.30%), Postives = 136/336 (40.48%), Query Frame = 0

Query:   55 QWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPT--FKLVSPYIPIRQGLQGCIQKLSINNSPRDL-------LRGAIAG------LNVNPCSH------------------------------PCSPVPNTQAPCGLHGTCRPNLEDFS-CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQ-----LNGNGQVFIGGVEDLEHL 337
            +WH + V+R  R   LAVD +    +  P   + L  + ++++GG P     +V+       GL+G I+ L +NN   DL       L G+  G       + NPC H                              PC P P     C +  TC    E  + C CP+G  G  C +      +   +P F G SYL LN  +    +     ++ +      P+G+I + G   DG     D++ L + +G+L +R++LG G  VL   E  +    W  +   R+ +   ++++NG    G SP K R+     LN     ++GG  D   L
Sbjct: 1469 KWHQLVVNRNRRSGMLAVDGEHVSGE-SPTGTDGLNLDTDLFVGGAPEDQMAVVAERTAATVGLKGSIRLLDVNNQMYDLREKGSDVLYGSGVGECGNDPCHPNPCHHGASCHVKEAEMFHCECLHSYTGPTCADERNPCDPTP-----CHISATCLVLPEGGAMCACPMGREGEFCERVTEQDHTMPFLPEFNGFSYLELNGLQTL-FLTCRQMSMEVVFLAKSPSGMIFYNGQKTDG---KGDFVSLALHDGYLEYRYDLGKGAAVLRSKEP-VPLNTWISVLLERSGRKGVMRINNGERVMGESP-KSRKVPHAFLNLKEPFYVGGAPDFSKL 1792          

BLAST of Pikachurin partial vs. SwissProt
Match: gi|1172451|sp|Q05793.1|PGBM_MOUSE (RecName: Full=Basement membrane-specific heparan sulfate proteoglycan core protein; Short=HSPG; Contains: RecName: Full=Endorepellin; Contains: RecName: Full=LG3 peptide; Flags: Precursor)

HSP 1 Score: 127.872 bits (320), Expect = 7.642e-31
Identity = 105/357 (29.41%), Postives = 165/357 (46.22%), Query Frame = 0

Query:   41 GDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQA-PCGLHGTCRPN-LEDFSCECPLGWVGIT------CSKKMNLTKSSAMIPRFGGRSY---LHLNR-----EE---------------LAKNILGNTNN---INIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL-PLPEFRHGLMGCIADISI-GRLFDI 361
            G  + R   P+ L Q+HT+ + R+     L V +    +    G F  L  N+ +YLGG P +  + P   +  G  GC+++L I           I   +VN  +H  S  P  Q  PC   G C+ +    ++C CP G+          C+   +L   +  + R  GR Y    HL R     EE               LA   L NT++   +++  K  +PNG++L++GG    P  D++ L ++ G L FR+ LG+G  VL  +E  +  GRWHR+ A R  +  SL+VD G     +SPGK + LN +  +++GGVE    L P         GC+ ++S+ G+  D+
Sbjct: 3060 GMATIRHPTPLALGQFHTVTLLRSLTQGSLIVGNLAPVNGTSQGKFQGLDLNEELYLGGYPDYGAI-PKAGLSSGFVGCVRELRIQGE-------EIVFHDVNLTTHGISHCPTCQDRPCQNGGQCQDSESSSYTCVCPAGFTAAAVNIRKPCTATPSLWADATCVNRPDGRGYTCRCHLGRSGVRCEEGVTVTTPSMSGAGSYLALPALTNTHHELRLDVEFKPLEPNGILLFSGGKS-GPVEDFVSLAMVGGHLEFRYELGSGLAVLRSHEP-LALGRWHRVSAERLNKDGSLRVDGGRPVLRSSPGKSQGLNLHTLLYLGGVEPSVQLSPATNMSAHFHGCVGEVSVNGKRLDL 3406          

HSP 2 Score: 123.635 bits (309), Expect = 2.476e-29
Identity = 96/362 (26.52%), Postives = 148/362 (40.88%), Query Frame = 0

Query:   46 RSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPC--SHPCSPVPNTQAPCGLHGTCRPNLE-DFSCECPLGWVGITCSKKMNLTK--------------SSAMIPRFGG------------------------------------RSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGG-DGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADI 353
            RS  P+ L +WH +   R  +   L VD  +   +  PG    L  +  +YLGGV     +SP   +     GC+ ++S+N    DL    +    V  C  S PC      + PC    TC P  E +F C C  G+ G  C  + N  +                  +P F G                                      +L L     ++++      I   ++ +  +GL+LW G     + + D++ LG+ +G L F + LG+GE  L+  +  INDG WHRI A R  Q  S++VD   + TG SPG    +N    ++IGG  D+  L   +F  G+ GCI ++
Sbjct: 3320 RSHEPLALGRWHRVSAERLNKDGSLRVDGGRPVLRSSPGKSQGLNLHTLLYLGGVEPSVQLSPATNMSAHFHGCVGEVSVNGKRLDLTYSFLGSQGVGQCYDSSPCE-----RQPCRNGATCMPAGEYEFQCLCQDGFKGDLCEHEENPCQLHEPCLNGGTCRGARCLCLPGFSGPRCQQGAGYGVVESDWHPEGSGGNDAPGQYGAYFYDNGFLGLPGNSFSRSLPEVPETIEFEVRTSTADGLLLWQGVVREASRSKDFISLGLQDGHLVFSYQLGSGEARLVSGDP-INDGEWHRITALREGQRGSIQVDGEDLVTGRSPGPNVAVNTKDIIYIGGAPDVATLTRGKFSSGITGCIKNL 3675          

HSP 3 Score: 55.8398 bits (133), Expect = 3.771e-7
Identity = 37/138 (26.81%), Postives = 67/138 (48.55%), Query Frame = 0

Query:  224 NINIRLKVTKPNGLILWTGGDGFTPAS------DYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISI 355
             I I  +    +G++L+ G    +P +      D++  G++ G   FRF+ G+G    I + T +  G++H +   R+    SL V N     G S GK + L+ N ++++GG  D   +P      G +GC+ ++ I
Sbjct: 3008 EIKITFRPDSADGMLLYNG-QKRSPTNLANRQPDFISFGLVGGRPEFRFDAGSGMAT-IRHPTPLALGQFHTVTLLRSLTQGSLIVGNLAPVNGTSQGKFQGLDLNEELYLGGYPDYGAIPKAGLSSGFVGCVRELRI 3143          

BLAST of Pikachurin partial vs. SwissProt
Match: gi|218563482|sp|A2ASQ1.1|AGRIN_MOUSE (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22)

HSP 1 Score: 125.946 bits (315), Expect = 3.289e-30
Identity = 97/342 (28.36%), Postives = 162/342 (47.37%), Query Frame = 0

Query:   46 RSEYPITLHQWHTIKVSRTGRLAFLAVDD------QKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNP-CSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPR--FGGRSYL-HLN---REELAKNILG---------------NTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDL---EHLPLPEFRHGLMGCIADISIG 356
            RS+ PI L  W  + + R GR   L V D      +  +S+  P     L   + +Y+GG P F  ++    +  G  G IQ +S+           +  ++V P   HPC+   +   PC   G+C P    + C CP G+ G+ C K + + KS   +    F GR+Y+ +LN     EL   I                  +N+  + L+     GL+LW G  G    +DY+ L +++G L   ++LG+ + V++ +  ++N  RW R+RA R  +  SL+V N    TG+SP    QL+ +G +++GG++ L   + LP   +  G +GC+ D+ +G
Sbjct: 1596 RSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTM--LNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDN--PCLNGGSCIPREATYECLCPGGFSGLHCEKGI-VEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVG--ERADYMALAIVDGHLQLSYDLGS-QPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALP-KAYGTGFVGCLRDVVVG 1928          

HSP 2 Score: 99.3673 bits (246), Expect = 3.066e-21
Identity = 92/335 (27.46%), Postives = 135/335 (40.30%), Query Frame = 0

Query:   50 PITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKL--VSPYIPIRQGLQGCIQKLSINNSP---RDLLRGAIAGLNVNPC-SHPCSPVPN---------------TQAPCGLHGT--------CRPNLEDFS------------CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLR----QLNGNGQVFIGGVEDLEHL 337
            P+   +WH +++SR  R   L+VD +       P   + L  +  +Y+GG+P  ++  V     +  GL+GCI+ L INN      D  R  +    V  C  HPCSP P                 Q P G  G         C+PN    S            C CPLG  G  C   +    S   +  F G SYL L      +  LG    + +      P+GL+L+ G   DG     D++ L +    L FR++LG G  ++   E  I  G W R+   R  +  +L+V +G    G SP   +     LN    +++GG  D   L
Sbjct: 1330 PVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDG---KGDFVSLALHNRHLEFRYDLGKGAAIIRSKEP-IALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKL 1660          

HSP 3 Score: 74.3294 bits (181), Expect = 4.119e-13
Identity = 61/209 (29.19%), Postives = 96/209 (45.93%), Query Frame = 0

Query:  158 PCGLHGTCRP--NLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLE---HLPLPEFRHGLMGCIADISIGRLFDI 361
            PC   GTC+   + + FSC C  G  G  C +K+ L      +P F G S+L             +T  + +  +  +  GL+L+ G        D+L L +L+G + FRF+ G+G  VL  +   +  GRWHR+  +R  +  +L VD      G SP     LN + ++++GG+ + +    L       GL GCI      R+ DI
Sbjct: 1219 PCLHGGTCQDLDSGKGFSCSCTAGRAGTVC-EKVQLPS----VPAFKGHSFLAFPTLRAY-----HTLRLALEFRALETEGLLLYNGN---ARGKDFLALALLDGHVQFRFDTGSGPAVLT-SLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCI------RMLDI 1407          

BLAST of Pikachurin partial vs. SwissProt
Match: gi|399021|sp|P25304.2|AGRIN_RAT (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22)

HSP 1 Score: 125.561 bits (314), Expect = 4.240e-30
Identity = 100/345 (28.99%), Postives = 163/345 (47.25%), Query Frame = 0

Query:   46 RSEYPITLHQWHTIKVSRTGRLAFLAVDD------QKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNP-CSHPCSPVPNTQA---PCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPR--FGGRSYL-HLN---REELAKNILG---------------NTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDL---EHLPLPEFRHGLMGCIADISIG 356
            RS+ PI L  W  + + R GR   L V D      +  +S+  P     L   + +Y+GG P F  ++    +  G  G IQ +S+           +  ++V+P   HPC     TQA   PC   G+C P    + C CP G+ G+ C K + + KS   +    F GR+Y+ +LN     EL   I                  +N+  + L+     GL+LW G       +DY+ L +++G L   ++LG+ + V++ +  ++N  RW RIRA R  +  SL+V N    TG+SP    QL+ +G +++GG++ L   + LP   +  G +GC+ D+ +G
Sbjct: 1605 RSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTM--LNLKEPLYIGGAPDFSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPC-----TQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGL-VEKSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGK--AAERADYMALAIVDGHLQLSYDLGS-QPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPK-AYGTGFVGCLRDVVVG 1937          

HSP 2 Score: 103.219 bits (256), Expect = 1.572e-22
Identity = 94/335 (28.06%), Postives = 134/335 (40.00%), Query Frame = 0

Query:   50 PITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPT--FKLVSPYIPIRQGLQGCIQKLSINNSP---RDLLRGAIAGLNVNPC-SHPCSPVPN---------------TQAPCGLHG--------TCRPN------------LEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLR----QLNGNGQVFIGGVEDLEHL 337
            P+   +WH +++SR  R   L+VD +       P   + L  + N+Y+GG+P     +V     +  GL+GCI+ L INN      D  R A+    V  C  HPC P P                 Q P G  G         C+PN                 CECPLG  G  C   +    S   +  F G SYL L      +  LG    + +      P+GL+L+ G   DG     D++ L +    L F ++LG G  V+   E  I  G W R+   R  +  +L+V +G    G SP   +     LN    ++IGG  D   L
Sbjct: 1339 PVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDG---KGDFVSLALHNRHLEFCYDLGKGAAVIRSKEP-IALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKL 1669          

HSP 3 Score: 71.633 bits (174), Expect = 3.725e-12
Identity = 60/210 (28.57%), Postives = 94/210 (44.76%), Query Frame = 0

Query:  158 PCGLHGTCRPNL--EDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRH----GLMGCIADISIGRLFDI 361
            PC   GTC+     + F+C C  G  G  C K    +     +P F G S+L             +T  + +  +  +  GL+L+ G        D+L L +L+G + FRF+ G+G  VL  +   +  GRWHR+  +R  +  +L VD      G SP     LN +  +++GG+ + E + +   R     GL GCI      R+ DI
Sbjct: 1228 PCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKVQPPS-----MPAFKGHSFLAFPTLRAY-----HTLRLALEFRALETEGLLLYNGN---ARGKDFLALALLDGRVQFRFDTGSGPAVLT-SLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPE-EQVAMVLDRTSVGVGLKGCI------RMLDI 1416          

BLAST of Pikachurin partial vs. SwissProt
Match: gi|30179887|sp|Q06561.2|UNC52_CAEEL (RecName: Full=Basement membrane proteoglycan; AltName: Full=Perlecan homolog; AltName: Full=Uncoordinated protein 52; Short=Protein unc-52; Flags: Precursor)

HSP 1 Score: 125.561 bits (314), Expect = 4.676e-30
Identity = 70/217 (32.26%), Postives = 114/217 (52.53%), Query Frame = 0

Query:  159 CGLHGTCRP-NLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGG-DGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            CG++G C P N+  ++CEC L + G TCS    L K      RF G +++ L+ +E           +  + K  + NG++LW G         DY+ +G++ G LHF + LG G   LI +E R++DG+ H +R  R  +   +++DN     G S G L  LN +G +F+GGV D+       F +  +GCIAD+ +  +  + +M  + DG+N+
Sbjct: 3146 CGMNGQCVPRNMTHYTCECKLYYDGPTCS----LFKPIEHAARFDGDAFIELSSDEFPHLTSEKDEIVAFKFKTEQQNGVLLWQGQRPTVQQMEDYISVGIVNGHLHFSYELGGGAAHLI-SEERVDDGKEHSVRFERKGREGQMRIDNYREVDGRSTGILAMLNVDGNIFVGGVPDISKATGGLFSNNFVGCIADVELNGV-KLDLMATAIDGKNV 3356          

BLAST of Pikachurin partial vs. nr
Match: gi|1325265600|ref|XP_023337213.1| (pikachurin-like [Eurytemora affinis])

HSP 1 Score: 327.405 bits (838), Expect = 8.779e-101
Identity = 160/348 (45.98%), Postives = 224/348 (64.37%), Query Frame = 0

Query:   26 MIQSAIAEHASSVPFGDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
             +     E A  +  G   ARS   + +  WH++ +SRTGR  +L++D ++ ++   PG F +L+ +QN++LGGVP + ++S Y+PI     GCIQKL +N  P  L+  A++GLNV  C H C       +PCG  G C PNL+ FSC+CPLGW G +CS     T  S+ IP F G SYL  N +++ +N++G  N+INIRL+VT  +GL++W GG+   P  DYL+LGV  G+L F FN+G+GE VL +N TRI+DG WHR+RATR EQTASL VDNG + TG++PG LRQLN    ++IGGVED   +    +  GL GCIA++SIG + +I M+  +  G NI
Sbjct:  550 FLNQGFVEFAFDLGNGIAIARSGSKVKIGSWHSLTISRTGREVYLSLDGEEGETVTSPGGFTQLSLSQNLWLGGVPEYGILSAYLPITTAFHGCIQKLVVNGLPIQLVGSALSGLNVESCVHSCQ-----GSPCGA-GICSPNLDTFSCQCPLGWGGESCSTP---TPDSSPIPGFTGNSYLFFNNQDIYQNLVGTANSINIRLRVTSAHGLLMWVGGEKSEPTPDYLLLGVHNGYLQFVFNVGSGEMVLEYNSTRIDDGLWHRVRATRLEQTASLMVDNGDVITGSAPGNLRQLNAKTGLYIGGVEDYSGISTHSYWTGLTGCIAELSIGNMINIDMLHTAERGGNI 888          

HSP 2 Score: 116.316 bits (290), Expect = 1.420e-24
Identity = 97/352 (27.56%), Postives = 167/352 (47.44%), Query Frame = 0

Query:   45 ARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINN-----SPRDLLRGAIAGLNVNPCSHP-CSPVPNTQAPCGLHGTCRPNLEDFS----------CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYL-HLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDL----EHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
             ++   + L+ W+ + V R     ++ ++  K +     G F+ +T NQ +Y+GG    + V  ++ + QGLQGC++ L IN+     +P      +I G++   C  P CS      + CG  GTCR     FS          C+CPLG  G  C ++ ++      +PRF G S+L H    + A   L     + I    ++  GLIL+ G   DG     D++ + + +GF+ F F+LGNG  +   + +++  G WH +  +RT +   L +D     T  SPG   QL+ +  +++GGV +      +LP+    H   GCI  + +  L  I+++  +  G N+
Sbjct:  338 VKTSSQVHLNAWNHVSVFRHDWGVWVQLNGGKREEGRSQGLFSRITFNQPVYIGGSGEMRNVKNFVGVDQGLQGCVRHLEINDLSYSFNPVSTGGDSIGGIDTGVCKTPGCS-----NSMCG-QGTCRDQSSPFSSNPANQSRQGCDCPLGSSGDRCQRQEHI-----QMPRFVGDSFLRHAGLGDAALIWL----EMEIVFNPSQLQGLILYNGNRNDG---KGDFVAVFLNQGFVEFAFDLGNGIAI-ARSGSKVKIGSWHSLTISRTGREVYLSLDGEEGETVTSPGGFTQLSLSQNLWLGGVPEYGILSAYLPITTAFH---GCIQKLVVNGL-PIQLVGSALSGLNV 666          

HSP 3 Score: 70.4774 bits (171), Expect = 1.995e-9
Identity = 56/210 (26.67%), Postives = 100/210 (47.62%), Query Frame = 0

Query:  157 APCGLH---GTC---RPNLEDFS-CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLP-LPEFRHGLMGCIADISIGRL 358
            A CGL+   GTC   R + E  S C CP GW G  C++ ++++     IP   G  ++     + A + L    ++++  K T   GL+L TG +      DYL L + +G++  R + G G GV +   ++++   W+ +   R +    ++++ G    G S G   ++  N  V+IGG  ++ ++        GL GC+  + I  L
Sbjct:  223 AFCGLNCGTGTCHTERVDTEWISRCICPAGWGGAQCAEHIDIS-----IPELSGTGFISFPTLQNAYSDL----HLSLEFKPTAWTGLLLLTG-ETEDMTGDYLALVLKDGYIELRLDCGTGPGV-VKTSSQVHLNAWNHVSVFRHDWGVWVQLNGGKREEGRSQGLFSRITFNQPVYIGGSGEMRNVKNFVGVDQGLQGCVRHLEINDL 421          

BLAST of Pikachurin partial vs. nr
Match: gi|1330884721|gb|PNF19359.1| (hypothetical protein B7P43_G06725 [Cryptotermes secundus])

HSP 1 Score: 308.916 bits (790), Expect = 8.838e-100
Identity = 144/334 (43.11%), Postives = 213/334 (63.77%), Query Frame = 0

Query:   41 GDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAM-IPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            G  + RS  P+TL +WH +++SRTGRLA L VD       M PGAF +L+  QN+Y+GGVP F +VSP + +R    GCIQK+ INN P  +L  A+ G+NV+ C HPC        PCG  G C P ++ F+CEC  G+    C +    T    + IPRF G SYLH +  +  + I+    NIN+R + T   GL+LW+G    + A D+L LG+ +G+LHFRFNLG+GE  + +N T+++DG WHR++A R EQ  S+ VDNG   T  SPGKLRQLN +  ++IGG+E+++H    ++++G++GCI+D+ +   + +K+++ S+ G+NI
Sbjct:   19 GVATIRSGEPVTLGEWHELRLSRTGRLAILQVDSMAPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVRVRTSFVGCIQKVVINNQPLQILAAALGGVNVDNCPHPC-----VTRPCGDEGRCVPEMDYFTCECAQGYHDTQCQQHAAPTMLQDIPIPRFAGNSYLHYSDPDTMRRIISYKININMRFRTTSSMGLLLWSGRCNMSAAGDFLALGIADGYLHFRFNLGSGEVTIPYNFTQVDDGLWHRVKAVRNEQEGSVTVDNGTTVTKRSPGKLRQLNTDTGLYIGGMENIDHASQNKYKNGMVGCISDLILDTDYHVKLVQMSTAGKNI 347          

BLAST of Pikachurin partial vs. nr
Match: gi|1228002413|ref|XP_021933090.1| (pikachurin isoform X2 [Zootermopsis nevadensis] >gi|1228002415|ref|XP_021933091.1| pikachurin isoform X2 [Zootermopsis nevadensis] >gi|1228002417|ref|XP_021933092.1| pikachurin isoform X2 [Zootermopsis nevadensis] >gi|1228002419|ref|XP_021933093.1| pikachurin isoform X2 [Zootermopsis nevadensis])

HSP 1 Score: 311.227 bits (796), Expect = 6.756e-96
Identity = 146/334 (43.71%), Postives = 215/334 (64.37%), Query Frame = 0

Query:   41 GDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAM-IPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            G  + RS  P+TL +WH +++SRTGRLA L VD+      M PGAF +L+  QN+Y+GGVP F +VSP + +R    GCIQK+ INN P  +L  A+ G+NV+ C HPC        PCG  G C P ++ F+CEC  G   + C +    T    + IPRF G SYLH +  +  + I+    NIN+R + +   GL+LW+G    + A D+L LG+ +G+LHFRFNLG+GE ++ +N TR++DG WHR++A R EQ  S+ VDNG      SPGKLRQLN N  ++IGG+E++EH  L ++++G++GCI+D+ +   + +K+++ S+ G+NI
Sbjct:  420 GAATIRSGEPVTLGEWHELRLSRTGRLAILQVDNMVPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVKVRTSFVGCIQKVVINNQPLQILAAALGGVNVDNCPHPC-----VTRPCGDEGHCVPEMDYFTCECAQGHHDVQCQQHAAPTILQDIPIPRFAGDSYLHYSDPDTMRRIISYKININMRFRTSSSVGLLLWSGRRNMSAAGDFLGLGIADGYLHFRFNLGSGEVIIPYNYTRVDDGLWHRVKAIRNEQEGSVTVDNGTTVAKRSPGKLRQLNTNTGLYIGGMENIEHASLNKYKNGMVGCISDLILDTDYHVKLVQMSTAGKNI 748          

HSP 2 Score: 128.642 bits (322), Expect = 7.669e-29
Identity = 94/319 (29.47%), Postives = 156/319 (48.90%), Query Frame = 0

Query:   45 ARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDL----LRGAIAGLNVNPCSHP-CSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL-PLPEFRHGLMGCIADISI 355
             RSE  + L+QW+ + + R    A++ +++ K       G F+ +T  + +++GG      ++  +P   GL+GCI+ L +N+         L  A+ G +V  CS   CS V     PC   G C  + E   C CPLG+ G  C  +++L      +P F G SYL      L  + L +  ++ + LK +  +G+IL+ G   DG     D++ + + +G L F F+LG G   +   E  +  G WH +R +RT + A L+VDN       +PG   QL+    ++IGGV + + + P  + R   +GCI  + I
Sbjct:  203 VRSEETVLLNQWNRLTLYRHRWDAWIQLNNGKHVEGRSKGLFSRITFREPLFVGGPGNTTGLADKLPTEDGLKGCIRHLEVNDHAYSFNLAPLGDAVKGFDVEECSADRCSKV-----PCQHGGKCLISGETAVCLCPLGFTGDLCETRVDL-----QVPSFNGSSYLRY--PGLGGSAL-SWLDLQLTLKPSAQDGVILYNGHRSDG---VGDFMAVYMSDGHLEFTFDLGTGAATIRSGEP-VTLGEWHELRLSRTGRLAILQVDNMVPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVKVRTSFVGCIQKVVI 504          

HSP 3 Score: 66.6254 bits (161), Expect = 2.970e-8
Identity = 55/205 (26.83%), Postives = 95/205 (46.34%), Query Frame = 0

Query:  159 CGLHGTCRPNLEDFS------CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLP--LPEFRHGLMGCIADISI 355
            CG+ G+C  +  D        C+CPLG  G  C   + +       PRF G+ +L       A   +     + +  +    +G++  TG +    A D++ L + +GF+ FRF+ G+G GV+   ET + + +W+R+   R    A ++++NG    G S G   ++     +F+GG  +   L   LP    GL GCI  + +
Sbjct:   91 CGVGGSCAMDAVDSDRPGEQRCQCPLGRGGRHCQADVEIRS-----PRFTGQGWLAFPALRAAYKHV----QLELEFRPEAWDGILFLTG-ERDDMAGDFMALLLHQGFVEFRFDCGSGLGVVRSEETVLLN-QWNRLTLYRHRWDAWIQLNNGKHVEGRSKGLFSRITFREPLFVGGPGNTTGLADKLPT-EDGLKGCIRHLEV 283          

BLAST of Pikachurin partial vs. nr
Match: gi|646703926|gb|KDR12352.1| (Pikachurin, partial [Zootermopsis nevadensis])

HSP 1 Score: 307.76 bits (787), Expect = 8.937e-96
Identity = 146/335 (43.58%), Postives = 216/335 (64.48%), Query Frame = 0

Query:   41 GDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAM-IPRFGGRSYLHLNR-EELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            G  + RS  P+TL +WH +++SRTGRLA L VD+      M PGAF +L+  QN+Y+GGVP F +VSP + +R    GCIQK+ INN P  +L  A+ G+NV+ C HPC        PCG  G C P ++ F+CEC  G   + C +    T    + IPRF G SYLH +  + + + I+    NIN+R + +   GL+LW+G    + A D+L LG+ +G+LHFRFNLG+GE ++ +N TR++DG WHR++A R EQ  S+ VDNG      SPGKLRQLN N  ++IGG+E++EH  L ++++G++GCI+D+ +   + +K+++ S+ G+NI
Sbjct:  295 GAATIRSGEPVTLGEWHELRLSRTGRLAILQVDNMVPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVKVRTSFVGCIQKVVINNQPLQILAAALGGVNVDNCPHPC-----VTRPCGDEGHCVPEMDYFTCECAQGHHDVQCQQHAAPTILQDIPIPRFAGDSYLHYSDPDTMRRQIISYKININMRFRTSSSVGLLLWSGRRNMSAAGDFLGLGIADGYLHFRFNLGSGEVIIPYNYTRVDDGLWHRVKAIRNEQEGSVTVDNGTTVAKRSPGKLRQLNTNTGLYIGGMENIEHASLNKYKNGMVGCISDLILDTDYHVKLVQMSTAGKNI 624          

HSP 2 Score: 128.642 bits (322), Expect = 7.172e-29
Identity = 94/319 (29.47%), Postives = 156/319 (48.90%), Query Frame = 0

Query:   45 ARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDL----LRGAIAGLNVNPCSHP-CSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL-PLPEFRHGLMGCIADISI 355
             RSE  + L+QW+ + + R    A++ +++ K       G F+ +T  + +++GG      ++  +P   GL+GCI+ L +N+         L  A+ G +V  CS   CS V     PC   G C  + E   C CPLG+ G  C  +++L      +P F G SYL      L  + L +  ++ + LK +  +G+IL+ G   DG     D++ + + +G L F F+LG G   +   E  +  G WH +R +RT + A L+VDN       +PG   QL+    ++IGGV + + + P  + R   +GCI  + I
Sbjct:   78 VRSEETVLLNQWNRLTLYRHRWDAWIQLNNGKHVEGRSKGLFSRITFREPLFVGGPGNTTGLADKLPTEDGLKGCIRHLEVNDHAYSFNLAPLGDAVKGFDVEECSADRCSKV-----PCQHGGKCLISGETAVCLCPLGFTGDLCETRVDL-----QVPSFNGSSYLRY--PGLGGSAL-SWLDLQLTLKPSAQDGVILYNGHRSDG---VGDFMAVYMSDGHLEFTFDLGTGAATIRSGEP-VTLGEWHELRLSRTGRLAILQVDNMVPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVKVRTSFVGCIQKVVI 379          

BLAST of Pikachurin partial vs. nr
Match: gi|1228002411|ref|XP_021933089.1| (pikachurin isoform X1 [Zootermopsis nevadensis])

HSP 1 Score: 311.227 bits (796), Expect = 1.241e-95
Identity = 146/334 (43.71%), Postives = 215/334 (64.37%), Query Frame = 0

Query:   41 GDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAM-IPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            G  + RS  P+TL +WH +++SRTGRLA L VD+      M PGAF +L+  QN+Y+GGVP F +VSP + +R    GCIQK+ INN P  +L  A+ G+NV+ C HPC        PCG  G C P ++ F+CEC  G   + C +    T    + IPRF G SYLH +  +  + I+    NIN+R + +   GL+LW+G    + A D+L LG+ +G+LHFRFNLG+GE ++ +N TR++DG WHR++A R EQ  S+ VDNG      SPGKLRQLN N  ++IGG+E++EH  L ++++G++GCI+D+ +   + +K+++ S+ G+NI
Sbjct:  441 GAATIRSGEPVTLGEWHELRLSRTGRLAILQVDNMVPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVKVRTSFVGCIQKVVINNQPLQILAAALGGVNVDNCPHPC-----VTRPCGDEGHCVPEMDYFTCECAQGHHDVQCQQHAAPTILQDIPIPRFAGDSYLHYSDPDTMRRIISYKININMRFRTSSSVGLLLWSGRRNMSAAGDFLGLGIADGYLHFRFNLGSGEVIIPYNYTRVDDGLWHRVKAIRNEQEGSVTVDNGTTVAKRSPGKLRQLNTNTGLYIGGMENIEHASLNKYKNGMVGCISDLILDTDYHVKLVQMSTAGKNI 769          

HSP 2 Score: 128.257 bits (321), Expect = 1.058e-28
Identity = 94/319 (29.47%), Postives = 156/319 (48.90%), Query Frame = 0

Query:   45 ARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDL----LRGAIAGLNVNPCSHP-CSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL-PLPEFRHGLMGCIADISI 355
             RSE  + L+QW+ + + R    A++ +++ K       G F+ +T  + +++GG      ++  +P   GL+GCI+ L +N+         L  A+ G +V  CS   CS V     PC   G C  + E   C CPLG+ G  C  +++L      +P F G SYL      L  + L +  ++ + LK +  +G+IL+ G   DG     D++ + + +G L F F+LG G   +   E  +  G WH +R +RT + A L+VDN       +PG   QL+    ++IGGV + + + P  + R   +GCI  + I
Sbjct:  224 VRSEETVLLNQWNRLTLYRHRWDAWIQLNNGKHVEGRSKGLFSRITFREPLFVGGPGNTTGLADKLPTEDGLKGCIRHLEVNDHAYSFNLAPLGDAVKGFDVEECSADRCSKV-----PCQHGGKCLISGETAVCLCPLGFTGDLCETRVDL-----QVPSFNGSSYLRY--PGLGGSAL-SWLDLQLTLKPSAQDGVILYNGHRSDG---VGDFMAVYMSDGHLEFTFDLGTGAATIRSGEP-VTLGEWHELRLSRTGRLAILQVDNMVPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVKVRTSFVGCIQKVVI 525          

HSP 3 Score: 66.6254 bits (161), Expect = 3.527e-8
Identity = 55/205 (26.83%), Postives = 95/205 (46.34%), Query Frame = 0

Query:  159 CGLHGTCRPNLEDFS------CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLP--LPEFRHGLMGCIADISI 355
            CG+ G+C  +  D        C+CPLG  G  C   + +       PRF G+ +L       A   +     + +  +    +G++  TG +    A D++ L + +GF+ FRF+ G+G GV+   ET + + +W+R+   R    A ++++NG    G S G   ++     +F+GG  +   L   LP    GL GCI  + +
Sbjct:  112 CGVGGSCAMDAVDSDRPGEQRCQCPLGRGGRHCQADVEIRS-----PRFTGQGWLAFPALRAAYKHV----QLELEFRPEAWDGILFLTG-ERDDMAGDFMALLLHQGFVEFRFDCGSGLGVVRSEETVLLN-QWNRLTLYRHRWDAWIQLNNGKHVEGRSKGLFSRITFREPLFVGGPGNTTGLADKLPT-EDGLKGCIRHLEV 304          

BLAST of Pikachurin partial vs. nr
Match: gi|1339068579|ref|XP_023721396.1| (pikachurin-like [Cryptotermes secundus])

HSP 1 Score: 306.605 bits (784), Expect = 7.588e-95
Identity = 144/334 (43.11%), Postives = 213/334 (63.77%), Query Frame = 0

Query:   41 GDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAM-IPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            G  + RS  P+TL +WH +++SRTGRLA L VD       M PGAF +L+  QN+Y+GGVP F +VSP + +R    GCIQK+ INN P  +L  A+ G+NV+ C HPC        PCG  G C P ++ F+CEC  G+    C +    T    + IPRF G SYLH +  +  + I+    NIN+R + T   GL+LW+G    + A D+L LG+ +G+LHFRFNLG+GE  + +N T+++DG WHR++A R EQ  S+ VDNG   T  SPGKLRQLN +  ++IGG+E+++H    ++++G++GCI+D+ +   + +K+++ S+ G+NI
Sbjct:  342 GVATIRSGEPVTLGEWHELRLSRTGRLAILQVDSMAPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVRVRTSFVGCIQKVVINNQPLQILAAALGGVNVDNCPHPC-----VTRPCGDEGRCVPEMDYFTCECAQGYHDTQCQQHAAPTMLQDIPIPRFAGNSYLHYSDPDTMRRIISYKININMRFRTTSSMGLLLWSGRCNMSAAGDFLALGIADGYLHFRFNLGSGEVTIPYNFTQVDDGLWHRVKAVRNEQEGSVTVDNGTTVTKRSPGKLRQLNTDTGLYIGGMENIDHASQNKYKNGMVGCISDLILDTDYHVKLVQMSTAGKNI 670          

HSP 2 Score: 123.635 bits (309), Expect = 4.017e-27
Identity = 92/319 (28.84%), Postives = 154/319 (48.28%), Query Frame = 0

Query:   45 ARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDL----LRGAIAGLNVNPCSHP-CSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL-PLPEFRHGLMGCIADISI 355
             RSE  + L+QW+ + + R    A++ +++ K       G F+ +T  + +++GG      ++  +P   GL+GCI+ L +N+         L  ++ G +V  CS   CS V     PC   G C  + E   C CPLG+ G  C  +++L      +P F G SYL      L  + L +  ++ + LK    +G+IL+ G   DG     D++ + + +G L F F+LG G   +   E  +  G WH +R +RT + A L+VD+       +PG   QL+    ++IGGV + + + P    R   +GCI  + I
Sbjct:  125 VRSEETVLLNQWNRLTLYRHRWDAWIQLNNGKHVEGRSKGLFSRITFREPLFVGGPGNTTGLAEKLPTEDGLRGCIRHLEVNDHVYRFNIAPLGDSVKGFDVEECSADRCSKV-----PCQHGGKCLTSGETAVCLCPLGFAGDLCETRVDL-----QVPSFNGSSYLRY--PGLGGSAL-SWLDLQLTLKPIAQDGVILYNGHRSDG---VGDFMAVYMSDGHLEFTFDLGTGVATIRSGEP-VTLGEWHELRLSRTGRLAILQVDSMAPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVRVRTSFVGCIQKVVI 426          

BLAST of Pikachurin partial vs. nr
Match: gi|1351630513|ref|XP_024082468.1| (pikachurin isoform X2 [Cimex lectularius] >gi|1351630515|ref|XP_024082469.1| pikachurin isoform X2 [Cimex lectularius])

HSP 1 Score: 289.656 bits (740), Expect = 8.884e-88
Identity = 142/329 (43.16%), Postives = 206/329 (62.61%), Query Frame = 0

Query:   41 GDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPAS-DYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESS 368
            G  + RS  P++L +WH + VSRTGRLA L +D + +   + PGAF +L    N+Y+GG+P F +VSP + IR    GCIQK++INN P  +L  A+AG+NVN C HPC        PCG    C P+ + + C C        C +  ++  S   +  F G +YLH    E+   +LGN  ++N+R + T P+GLI+WTG      AS D+L LGV +G+LH R+NLG+GE +LIFN+TRI+DG WHR++ATR+EQ  +L VD+G   T  SPGKL+QLN N  ++IGG+ED+E     ++  G MGCI+++++   + IK++  S 
Sbjct:  409 GTATVRSPEPLSLGEWHEVMVSRTGRLAVLQIDKKPSTQILSPGAFTQLYLPLNLYIGGIPNFDMVSPKVKIRTSFVGCIQKITINNQPLQILAEALAGVNVNNCPHPC-----VAKPCGDVAHCVPHYDAYKCVC-----DKHCEENNDI--SPTHVASFSGTTYLHYTDPEILNRVLGNKVSLNMRFRTTSPSGLIVWTGRTEVRQASGDFLALGVRDGYLHLRYNLGSGETLLIFNDTRIDDGNWHRVKATRSEQEGTLAVDHGKTITSRSPGKLKQLNTNTGLYIGGMEDMELATHYKYHKGFMGCISELTLNSDYHIKLVWTSD 725          

HSP 2 Score: 119.398 bits (298), Expect = 1.095e-25
Identity = 94/337 (27.89%), Postives = 159/337 (47.18%), Query Frame = 0

Query:   45 ARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLR----GAIAGLNVNPC-SHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL-PLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
             RS+  + L+QW+ I + R    A++ ++  K       G F+ +T  + +++GG      +   +P   G +GCI+ L IN+            A+ G ++  C +  CS V     PC   G C  + +   C CPLG+ G  C  +++L      +P F G SYL      L  + L +  +I I LK    NG+IL+ G   DG     D++ + + +G L F F+LG G   +   E  ++ G WH +  +RT + A L++D        SPG   QL     ++IGG+ + + + P  + R   +GCI  I+I     ++++ E+  G N+
Sbjct:  192 VRSQETVILNQWNRITLYRHRWDAWVQLNSGKHVQGRSKGLFSRMTFREPVFIGGRGNTSGLQEKLPTDHGFKGCIRHLQINDYLYKFAAVPKGDAVKGFDIEECIADRCSKV-----PCQHGGKCLTSDDSAVCLCPLGFTGDLCETRLDL-----QVPAFNGSSYLRY--PGLGSSAL-SWLDILIVLKPNDLNGVILYNGHRSDG---VGDFIAMYLCDGHLEFTFDLGTGTATVRSPEP-LSLGEWHEVMVSRTGRLAVLQIDKKPSTQILSPGAFTQLYLPLNLYIGGIPNFDMVSPKVKIRTSFVGCIQKITINNQ-PLQILAEALAGVNV 510          

HSP 3 Score: 74.7146 bits (182), Expect = 8.166e-11
Identity = 60/208 (28.85%), Postives = 100/208 (48.08%), Query Frame = 0

Query:  152 VPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDL----EHLPLPEFRHGLMGCIADISI 355
            +P     CG +G C   ++   C+CPLG  G+ C  ++++       PRF G+ +L     + A   +     + I  +    +G++  TG +    A D++ L + +GF+ FRF+ G+G GV+   ET I + +W+RI   R    A +++++G    G S G   ++     VFIGG  +     E LP     HG  GCI  + I
Sbjct:   82 LPQPCEDCG-NGNCL--MDTSRCQCPLGTSGVHCQSEIDI-----RTPRFSGQGWLAFPALKAAYKHV----QLEIEFRPEVWDGILFLTG-ERDDLAGDFMALLIHQGFIEFRFDCGSGVGVVRSQETVILN-QWNRITLYRHRWDAWVQLNSGKHVQGRSKGLFSRMTFREPVFIGGRGNTSGLQEKLPT---DHGFKGCIRHLQI 272          

BLAST of Pikachurin partial vs. nr
Match: gi|939237246|ref|XP_014260047.1| (pikachurin isoform X1 [Cimex lectularius])

HSP 1 Score: 290.041 bits (741), Expect = 1.923e-87
Identity = 142/329 (43.16%), Postives = 206/329 (62.61%), Query Frame = 0

Query:   41 GDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPAS-DYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESS 368
            G  + RS  P++L +WH + VSRTGRLA L +D + +   + PGAF +L    N+Y+GG+P F +VSP + IR    GCIQK++INN P  +L  A+AG+NVN C HPC        PCG    C P+ + + C C        C +  ++  S   +  F G +YLH    E+   +LGN  ++N+R + T P+GLI+WTG      AS D+L LGV +G+LH R+NLG+GE +LIFN+TRI+DG WHR++ATR+EQ  +L VD+G   T  SPGKL+QLN N  ++IGG+ED+E     ++  G MGCI+++++   + IK++  S 
Sbjct:  462 GTATVRSPEPLSLGEWHEVMVSRTGRLAVLQIDKKPSTQILSPGAFTQLYLPLNLYIGGIPNFDMVSPKVKIRTSFVGCIQKITINNQPLQILAEALAGVNVNNCPHPC-----VAKPCGDVAHCVPHYDAYKCVC-----DKHCEENNDI--SPTHVASFSGTTYLHYTDPEILNRVLGNKVSLNMRFRTTSPSGLIVWTGRTEVRQASGDFLALGVRDGYLHLRYNLGSGETLLIFNDTRIDDGNWHRVKATRSEQEGTLAVDHGKTITSRSPGKLKQLNTNTGLYIGGMEDMELATHYKYHKGFMGCISELTLNSDYHIKLVWTSD 778          

HSP 2 Score: 119.783 bits (299), Expect = 9.766e-26
Identity = 94/337 (27.89%), Postives = 159/337 (47.18%), Query Frame = 0

Query:   45 ARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLR----GAIAGLNVNPC-SHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL-PLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
             RS+  + L+QW+ I + R    A++ ++  K       G F+ +T  + +++GG      +   +P   G +GCI+ L IN+            A+ G ++  C +  CS V     PC   G C  + +   C CPLG+ G  C  +++L      +P F G SYL      L  + L +  +I I LK    NG+IL+ G   DG     D++ + + +G L F F+LG G   +   E  ++ G WH +  +RT + A L++D        SPG   QL     ++IGG+ + + + P  + R   +GCI  I+I     ++++ E+  G N+
Sbjct:  245 VRSQETVILNQWNRITLYRHRWDAWVQLNSGKHVQGRSKGLFSRMTFREPVFIGGRGNTSGLQEKLPTDHGFKGCIRHLQINDYLYKFAAVPKGDAVKGFDIEECIADRCSKV-----PCQHGGKCLTSDDSAVCLCPLGFTGDLCETRLDL-----QVPAFNGSSYLRY--PGLGSSAL-SWLDILIVLKPNDLNGVILYNGHRSDG---VGDFIAMYLCDGHLEFTFDLGTGTATVRSPEP-LSLGEWHEVMVSRTGRLAVLQIDKKPSTQILSPGAFTQLYLPLNLYIGGIPNFDMVSPKVKIRTSFVGCIQKITINNQ-PLQILAEALAGVNV 563          

HSP 3 Score: 74.7146 bits (182), Expect = 8.212e-11
Identity = 60/208 (28.85%), Postives = 100/208 (48.08%), Query Frame = 0

Query:  152 VPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDL----EHLPLPEFRHGLMGCIADISI 355
            +P     CG +G C   ++   C+CPLG  G+ C  ++++       PRF G+ +L     + A   +     + I  +    +G++  TG +    A D++ L + +GF+ FRF+ G+G GV+   ET I + +W+RI   R    A +++++G    G S G   ++     VFIGG  +     E LP     HG  GCI  + I
Sbjct:  135 LPQPCEDCG-NGNCL--MDTSRCQCPLGTSGVHCQSEIDI-----RTPRFSGQGWLAFPALKAAYKHV----QLEIEFRPEVWDGILFLTG-ERDDLAGDFMALLIHQGFIEFRFDCGSGVGVVRSQETVILN-QWNRITLYRHRWDAWVQLNSGKHVQGRSKGLFSRMTFREPVFIGGRGNTSGLQEKLPT---DHGFKGCIRHLQI 325          

BLAST of Pikachurin partial vs. nr
Match: gi|939660408|ref|XP_014278124.1| (PREDICTED: pikachurin [Halyomorpha halys])

HSP 1 Score: 280.026 bits (715), Expect = 3.858e-84
Identity = 138/327 (42.20%), Postives = 198/327 (60.55%), Query Frame = 0

Query:   41 GDLSARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIP---RFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPAS-DYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKM 363
            G  + RS  P++L +WH + +SRTGRLA L VD   +   + PGAF +L    N+Y+GG+P F +VSP + +R    GCIQK+ INN P  +L  A+AG+NV+ C HPC        PCG    C P+ + + C C           K     S  MIP    F G ++LH    E+   IL N   IN+RL+ T P+GLILWTG    + AS DYL LGV +G+LH R+NLG+GE  ++FN+T+I+DG WHR++ATR EQ  SL +D+G   T  SPGKL+QLN N  +++GG+E++E     ++  G  GC++++++   + IK+
Sbjct:  406 GAATIRSSEPLSLGEWHEVTISRTGRLAVLQVDKNPSAQILSPGAFTQLYLPLNIYIGGIPNFDMVSPKVKVRTSFVGCIQKILINNRPLQILAEALAGVNVDNCPHPC-----VAKPCGDVAHCVPHFDFYKCVC----------DKHCEENSQLMIPPVASFTGNTHLHYTDPEILNRILSNKVGINMRLRTTSPSGLILWTGRSDLSQASSDYLALGVKDGYLHMRYNLGSGETFIVFNDTKIDDGMWHRVKATRNEQEGSLTIDHGETVTSKSPGKLKQLNTNTGLYVGGIEEMEKSTHYKYHKGFSGCVSELTLNSDYHIKL 717          

HSP 2 Score: 119.783 bits (299), Expect = 8.387e-26
Identity = 98/339 (28.91%), Postives = 164/339 (48.38%), Query Frame = 0

Query:   45 ARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINN-----SPRDLLRG-AIAGLNVNPC-SHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTG--GDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL-PLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
             RS+  + L+QW+ I + R    A++ ++  K       G F+ +T  + +++GG      +   +P   GL+GC++ L IN+     SP  + +G A+ G ++  C +  CS V     PC   G C  + +   C CPLG+ G  C  +++L      +P F G SYL      L  + L +  +I + LK    NG+IL+ G   DG     D++ L   +G L F F+LG G   +  +E  ++ G WH +  +RT + A L+VD        SPG   QL     ++IGG+ + + + P  + R   +GCI  I I     ++++ E+  G N+
Sbjct:  189 VRSQETVVLNQWNRITLYRHRWDAWIQLNSGKHVQGRSKGLFSRMTFREPVFIGGRGNTSGLQDKLPTDHGLKGCVRHLQINDYVYKFSP--VPKGDAVKGFDIEECIADRCSKV-----PCQHGGKCLTSGDSAVCLCPLGFTGDLCETRLDL-----QVPAFNGSSYLRY--PGLGGSAL-SWLDIVVVLKPNALNGVILYNGHRADGM---GDFIALYTSDGHLEFTFDLGTGAATIRSSEP-LSLGEWHEVTISRTGRLAVLQVDKNPSAQILSPGAFTQLYLPLNIYIGGIPNFDMVSPKVKVRTSFVGCIQKILINNR-PLQILAEALAGVNV 507          

HSP 3 Score: 73.559 bits (179), Expect = 1.899e-10
Identity = 55/184 (29.89%), Postives = 91/184 (49.46%), Query Frame = 0

Query:  174 CECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHL--PLPEFRHGLMGCIADISI 355
            C+CPLG VG  C   +++      IPRF G+ +L     + A   +     + I  +    +G++  TG +      D++ L + +GF+ FRF+ G+G GV+   ET + + +W+RI   R    A +++++G    G S G   ++     VFIGG  +   L   LP   HGL GC+  + I
Sbjct:   98 CQCPLGKVGPHCKTDVDI-----QIPRFSGQGWLAFPALKAAYKHV----QLEIEFRPEVYDGILFLTG-ERDDLVGDFMALLLHQGFVEFRFDCGSGVGVVRSQETVVLN-QWNRITLYRHRWDAWIQLNSGKHVQGRSKGLFSRMTFREPVFIGGRGNTSGLQDKLPT-DHGLKGCVRHLQI 269          

BLAST of Pikachurin partial vs. nr
Match: gi|1173902301|gb|OQR72153.1| (pikachurin-like, partial [Tropilaelaps mercedesae])

HSP 1 Score: 271.937 bits (694), Expect = 4.955e-84
Identity = 128/329 (38.91%), Postives = 200/329 (60.79%), Query Frame = 0

Query:   45 ARSEYPITLHQWHTIKVSRTGRLAFLAVDDQKTQSQMGPGAFNELTANQNMYLGGVPTFKLVSPYIPIRQGLQGCIQKLSINNSPRDLLRGAIAGLNVNPCSHPCSPVPNTQAPCGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDLEHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
             RS+  +T+ +WHTI +SRTG+L  L VD         PGAF +L+ + N+Y+GGVP+FK  SP   I     GCIQK++INN P  LL+ A+ G NVN C+HPC        PC   G C P +++F C C LG+ G  C  ++   + +   P  GG+S+LH   +E+ + + G+  +I ++ +    +GL+LW G    + ++D++ LG+ +G L FRFNLG+GEG L++N +R++DG WH +RA R  Q  SL VD+G    G S G+L QL  N  +F+GG+ DL H+ L  ++ GL+GCI + ++   + ++++  ++ G N+
Sbjct:  119 VRSDEVVTVDKWHTISISRTGQLGILRVDKHPYIVGFAPGAFTQLSLDLNLYIGGVPSFKETSPNAAITHNFAGCIQKVTINNKPLKLLQEALTGRNVNNCAHPC-----VTGPCYNGGQCEPTMDEFQCHCKLGFSGQQCEIEV---QEAIAQPMMGGQSFLHYTADEIIRRVRGSKIDIQMKFRSFSGHGLLLWAGEKDVSHSTDFVSLGINKGQLVFRFNLGSGEGDLVYNYSRVDDGHWHTLRAIRYRQEGSLTVDDGPTIVGTSFGQLNQLTVNSGLFLGGIRDLTHMTLGRYQTGLVGCIGNFTLSTDYHVRLVTHANSGINV 439          

HSP 2 Score: 105.145 bits (261), Expect = 2.834e-21
Identity = 69/216 (31.94%), Postives = 109/216 (50.46%), Query Frame = 0

Query:  159 CGLHGTCRPNLEDFSCECPLGWVGITCSKKMNLTKSSAMIPRFGGRSYLHLNREELAKNILGNTNNINIRLKVTKPNGLILWTGGDGFTPASDYLMLGVLEGFLHFRFNLGNGEGVLIFNETRINDGRWHRIRATRTEQTASLKVDNGHIRTGASPGKLRQLNGNGQVFIGGVEDL-EHLPLPEFRHGLMGCIADISIGRLFDIKMMEESSDGRNI 373
            C   G C  + +  SC CPLG+ G  C +++ +     ++P F G S++      L +  L     I + LK    NG+  + G D     SD++ML ++ GF+ FRF+LGNG  ++  +E    D +WH I  +RT Q   L+VD      G +PG   QL+ +  ++IGGV    E  P     H   GCI  ++I     +K+++E+  GRN+
Sbjct:   12 CQNGGQCVDHGKTASCICPLGYEGEFCDQEVTI-----LVPAFNGSSFMRF--PGLGERFLSFLE-ITVVLKPRSLNGVFFYNG-DSLEGQSDFIMLQLVNGFVEFRFDLGNGAAIVRSDEVVTVD-KWHTISISRTGQLGILRVDKHPYIVGFAPGAFTQLSLDLNLYIGGVPSFKETSPNAAITHNFAGCIQKVTINNK-PLKLLQEALTGRNV 216