af190129_1na+ k+ 2cl- cotransporter, maker-scaffold1253_size52701-snap-gene-0.12 (gene) Tigriopus kingsejongensis
|
Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000003059 (supercontig:LSalAtl2s:LSalAtl2s1734:14347:19068:1 gene:EMLSAG00000003059 transcript:EMLSAT00000003059 description:"maker-LSalAtl2s1734-augustus-gene-0.5") HSP 1 Score: 1278.46 bits (3307), Expect = 0.000e+0 Identity = 672/1127 (59.63%), Postives = 851/1127 (75.51%), Query Frame = 0
Query: 66 GECPSGSDPSFPD---VPECLTPSPYLP---TRNGN--------RKRSSVTIA-PGTILEKPLPSQRKMTSKQRSSFILTPKPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVER-INDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGL----ASPATLTKRKLGRDGSVLGR--------------FQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNK-SIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMI---TIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
G PSG D +F + + + ++ YL R+ N R+R SVTI+ P EKP PS R+++ K+ S T + L+HYLT+E +PH DHYRN++SF+KG P+ SRPTLD+LL++ S N +D E + +D G IKFGW +GVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGL IITLSNIVTVIT+ISMSAVSTNGQIKAGGIY+MISRSLGPEFGGAIG+MFTLANSIAV+MYIIGFCE+L DML+QY+ F GI+++ E ++D+RLIGS+SLVL+L LAIVGMEWVTRVQ GLL+LLL SQ+DFIIGS +P S++++AKGFVGY+ VL NL++ Y +E + ++FF+VFAVFFPAVTGIVAGANLSGDLKDP VAIPKGTLL IV+TYVTY IYG+M+A + R ASG+V E+ D N+T+ E+ NIT ++ +CE R C+YGIL SQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRV QAVAKDKLFPGI SF++GWG NNDPV+GY+LVF+ISLICILIGDLN VSSLLSNFFVAAYALINFSVFHASIT SPGWRP FKYYNKWISLLGT+LCI VMFLMD TA++T + + LY+++ RKP+VNWGSS+Q+QSF+TAL +V +LT+V+DHVKNYRPKIMV+SG+P++RP+L+DFA+L+TKR+SLLES+ + LD+KK A+K++ W NHIK F S+ RN + EGAR A+E+ GLGKL NM+L+GF+ W + A+EYY+ ++TAFD+RLAVGVLRI+GGLD++ M E +S +LN++D ES MSVDSGL S A T + G D G F+KK SS+ L +KD +P++E+ V +++QFR+K ++ +GTID+YWLYDDG HIL TR K++K K+R+FFLS DELD TR+M +LL+KFRI+F DVI+L D TK+ K+T+D+FKAM+ ++ + SG+ +S + + + D + LV+E ++ + E+TNF LRIAEIV+ NSS ADL+VMTLP+PK+E +P LYMAWLD+TT+ MPPFL+VRGNQ SVLT
Sbjct: 27 GGDPSGGDDNFSENGSIDDSISYKNYLAYELNRSSNPGGGGGGDRRRPSVTISVPEN--EKPSPSSRRLSRKRSSFQFTTQRSLRHYLTREAIPHADHYRNKMSFAKGKPDSSRPTLDDLLNDDPYSKDN-SDLE---SQTDDQDTGSVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLAIITLSNIVTVITSISMSAVSTNGQIKAGGIYFMISRSLGPEFGGAIGLMFTLANSIAVAMYIIGFCEALSDMLKQYIPGFIGILNTPEDGLHDIRLIGSVSLVLLLGLAIVGMEWVTRVQKGLLVLLLFSQLDFIIGSFLPPSEDKLAKGFVGYNGTVLMENLFSEYIPNEGTEKNNSFFTVFAVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLXIVSTYVTYTIYGVMIAAXTXREASGDVLEL----DYVNETIKEVNNITNSFWECENRTCSYGILHSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVFQAVAKDKLFPGIASFADGWGKNNDPVKGYLLVFLISLICILIGDLNYVSSLLSNFFVAAYALINFSVFHASITKSPGWRPGFKYYNKWISLLGTVLCICVMFLMDMVTALVTFIIVFFLYMYVSIRKPEVNWGSSTQAQSFMTALNSVLSLTKVEDHVKNYRPKIMVMSGSPLNRPTLIDFANLVTKRISLLESVDVVPEDLDWKKSQAIKNSGQNWFTENHIKGFFSVVRNSKISEGARTAMELSGLGKLRTNMILLGFQDTWWKKDESAKEYYKIIQTAFDLRLAVGVLRINGGLDVTSMTNLMED----------SSPNQSNALNYDDRCESNMSVDSGLDLATKSGAKNTSTQSGEDDKEDGSLKRNKKPNGFEKLLFKKKCPSSIPLPPSEKDVKPLDEDIVNRLSQFRDKTTVNTGTIDVYWLYDDGXXXX---HILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVP-PLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLIVMTLPMPKKEIVPQELYMAWLDYTTKDMPPFLLVRGNQTSVLT 1129 HSP 2 Score: 1021.92 bits (2641), Expect = 0.000e+0 Identity = 548/1053 (52.04%), Postives = 717/1053 (68.09%), Query Frame = 0
Query: 1120 REQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPA-RFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLR-LFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELY---------FQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNV-------DSFGTSSTRNNSEVSY-----------------GIRDGNPIDERIVYRMTQFRDKSQ-KVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGD---------------------EQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RE IP+ D KM +G DS SRPTLD+LL N Y + +S T D +DTG +FGWI+GVY+ CLLNIWGVMLFLRLTWV+GQAGLL AIIT++N+VT +T+ISMSAV TNGQIK GGIYFMISRSLGPEFGGA+GLMFT A SIAV+MYIIGFCEAL DM+ Q+ F GI+ E +D+R++GS +LVLLLG++ +GM V ++Q LL++LL SQ+DF+IG+F+PPS++K A G VG++ +L NL+ ++ N E+ F VFAVFFPAVTG+VAGAN+SGDLKDPG AIPKGTL I++TY +Y +Y ++IAA R ASG+ EL D +E F C NR C+YG L +QQM+++ S WGPLIYAGCFAATLSSA+ASLVG PRVFQA+AKDKLFP I SF G+G++ +P++GYLL+F I+L CILIGDLN VSSLLSNFFVA YA+IN+SVFHAS SPGWRP FKY+NKWISLLGT LCI VM LMD+ TAL T I + LY++V R P+VNWGSS+Q+QSF+TAL S+ +L VEDHVKNYRPK++V++G+P +RP L DFANL+TKR SLLE V V+ ED++ + +K +W +NHIK F+++ RN G RTA+ELSGLGKL NM+++GFQ+ WW + +EYY+++ +FD+ L+VGVLR+ GGLD++ ++ E ++ +Q++ + +DR +S+D + + +KN D S RN + +D P+DE IV R++QFRDK+ GTIDVYWLYDDG +IL TR K++KCKMRVFFLSN+ D L + MASLL KFRI+F+DVI+L+D TK PKKET F+ M++ + E P+VSE++++ + ++T F+LRIAEI K+NS + L+VMTLP+P++ +P LY+AWLD+ ++ MPPFL VRGNQ SVLTFYS
Sbjct: 114 REAIPHA------DHYRNKMS---FAKGKPDS---SRPTLDDLL----NDDPYSKDNSDLESQTDD--------QDTGSVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLAIITLSNIVTVITSISMSAVSTNGQIKAGGIYFMISRSLGPEFGGAIGLMFTLANSIAVAMYIIGFCEALSDMLKQYIPGFIGILNTPEDGLHDIRLIGSVSLVLLLGLAIVGMEWVTRVQKGLLVLLLFSQLDFIIGSFLPPSEDKLAKGFVGYNGTVLMENLFSEYIPNEGTEKNNSFFTVFAVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLXIVSTYVTYTIYGVMIAAXTXREASGDVLEL--DYVNETIKEVNNITNSFWECENRTCSYGILHSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVFQAVAKDKLFPGIASFADGWGKNNDPVKGYLLVFLISLICILIGDLNYVSSLLSNFFVAAYALINFSVFHASITKSPGWRPGFKYYNKWISLLGTVLCICVMFLMDMVTALVTFIIVFFLYMYVSIRKPEVNWGSSTQAQSFMTALNSVLSLTKVEDHVKNYRPKIMVMSGSPLNRPTLIDFANLVTKRISLLESVDVVPEDLDWKKSQAIKNSGQNWFTENHIKGFFSVVRNSKISEGARTAMELSGLGKLRTNMILLGFQDTWWKKDESAKEYYKIIQTAFDLRLAVGVLRINGGLDVTSMTNLMED--SSPNQSNALNYDDR---CESNMSVDSGLDLATKSGAKNTSTQSGEDDKEDGSLKRNKKPNGFEKLLFKKKCPSSIPLPPSEKDVKPLDEDIVNRLSQFRDKTTVNTGTIDVYWLYDDGXXXX---HILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVPPLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLIVMTLPMPKKEIVPQELYMAWLDYTTKDMPPFLLVRGNQTSVLTFYS 1132
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000009267 (supercontig:LSalAtl2s:LSalAtl2s58:680408:685072:1 gene:EMLSAG00000009267 transcript:EMLSAT00000009267 description:"augustus_masked-LSalAtl2s58-processed-gene-6.1") HSP 1 Score: 514.998 bits (1325), Expect = 5.586e-159 Identity = 336/695 (48.35%), Postives = 429/695 (61.73%), Query Frame = 0
Query: 113 LPSQRKMTSKQRSSFILTP-----KPLKHYLTKEVLPHVDHYRNRLSFSKGHPER-SRPTL----------DELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRK----CNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQ----VSSLLSNFFVAAYALI-----------NFSVFHASITSSPGWRPSF-KYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKV-NWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVL--SGNPVDRPSLMD-FAHLLTKRLSLLESIQI--TEASLDFKKV 765
+P K ++ +SF T K + YLT++ LP HYRN LS S HP + SRPTL LLD+ + D+ N+ E+ S+GK ++FGW +GV+MRCLLNIWGVMLFLRLTWV+GQ GL++GLG+I L N+VTVITT+SMSAVSTNGQI+ GGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFC+SL+DM +QY + ++ S + NDVR+IG+ISLV +LALAIVGMEWVTRVQM LL LL+ SQ+DF++GS +P+ E+ A GFVGY+ + NL Y + G +FFSVF VFFPAVTGIVAGANLSGDLKDP VAIPKGTLLAI+ T++TY+ YG ++ ASGN E + N G + +++C GR C YG QQ ME IS G LIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+P IH F+ G+GANNDP+RGY LVFIIS+ CILI +Q V L FV I N+ T P RP + N L ++C V + A + ++ V ++FR K+ ++ S +Q+++F A L + RP +++ G+ ++ P +D + +L L + I E+ DF KV
Sbjct: 36 VPDHDKAGGEETNSFHPTYSTQYFKSFRQYLTRDALPSESHYRNLLSISN-HPRKFSRPTLEELQEEQAENGRLLDKKDMDDVEKNE--------EEQSKGKVVRFGWIEGVFMRCLLNIWGVMLFLRLTWVVGQGGLIQGLGVIALGNLVTVITTLSMSAVSTNGQIRGGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCQSLLDMCKQYF-ELEHLIFS-DTTNDVRIIGAISLVAVLALAIVGMEWVTRVQMILLFLLIGSQIDFVVGSFLPTK-EDAAYGFVGYNGTLFVDNLSPTYHDYKDPGNPPSFFSVFGVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLAIIVTFITYIGYGTIITGVMHTEASGNQTEYE---NWINGVEGALY-----FNNCSGRPADDFCQYGSSNDQQAMERISYTGYLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIHIFAPGFGANNDPIRGYFLVFIISMGCILIATESQRLTGVVQLKXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHV----NIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLA----GIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKV 702 HSP 2 Score: 419.853 bits (1078), Expect = 1.735e-125 Identity = 238/463 (51.40%), Postives = 304/463 (65.66%), Query Frame = 0
Query: 1185 DCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVD-GEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEEL---LLDREDELYFQNCSNRN----CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGD----LNRVSSLLSNFFVATYAVINYSVFHASWNNSP----GWRPSFKYF 1631
D + + + RFGWI+GV++ CLLNIWGVMLFLRLTWVVGQ GL+ +I + NLVT +T +SMSAV TNGQI+GGGIY+MISRSLGPEFGGA+G+MFT A SIAVSMYIIGFC++LLDM Q+ I D+ NDVRI+G+ +LV +L ++ +GM V ++Q++LL +L+ SQ+DF++G+F+ P+ E A G VG++ L NL P + D G F VF VFFPAVTG+VAGAN+SGDLKDPG AIPKGTL AI+ T+ +Y+ Y +I ASGN E + E LYF NCS R C YG+ +QQ ++ S G LIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P+I F GFG + +P+RGY L+F I++ CILI L V L FV T I S N++P +RP F
Sbjct: 116 DVEKNEEEQSKGKVVRFGWIEGVFMRCLLNIWGVMLFLRLTWVVGQGGLIQGLGVIALGNLVTVITTLSMSAVSTNGQIRGGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCQSLLDMCKQYFELEHLIFSDT---TNDVRIIGAISLVAVLALAIVGMEWVTRVQMILLFLLIGSQIDFVVGSFL-PTKEDAAYGFVGYNGTLFVDNLSPTYHDYKDPGNPPSFFSVFGVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLAIIVTFITYIGYGTIITGVMHTEASGNQTEYENWINGVEGALYFNNCSGRPADDFCQYGSSNDQQAMERISYTGYLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIHIFAPGFGANNDPIRGYFLVFIISMGCILIATESQRLTGVVQLKXQQFVTTLKSIQ------SLNDTPEHVKNYRPKVLVF 568 HSP 3 Score: 345.895 bits (886), Expect = 2.637e-100 Identity = 185/433 (42.73%), Postives = 263/433 (60.74%), Query Frame = 0
Query: 1683 QSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+ Q F+T LKS+Q+LN +HVKNYRPK++V TG P+ RP L DFANLITK+ SLL HV D + ++ ++ V W RD+ IK+FY+LT+N +F G + L+G+GK+ NM++ G++ W PK +EY VL +FD+HL VG+LR++ G D S + G E+ DE Q KS++L K V R S Y DGNP+ + V +TQF+ K +K G IDV+WLYDDGGLTLLLPYIL+TR +FA+C++RVF L+NR D L E MA LL KFRI + V ++ DVTK T F+ ++ DE +P + E ++ ++T HLR+ E+ +E+S S +++MTL +PR+ LY++WL+ ++++MPPFL +RGNQ SVLTFYS
Sbjct: 537 KXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHVNIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLAGIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKVIGEEEQQQHEIELDEENEDEKNGK-----------QGKSLELQQKKV------RXRKVSTAVYTGIDGNPLPKNTVQDITQFQIKKRK-GNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKIL--DE---IKPPLEESEIISQKEKTNRHLRLTELLREHSKRSEMIIMTLSIPRRGTAVAQLYMSWLEMMTKNMPPFLLIRGNQSSVLTFYS 946 HSP 4 Score: 337.421 bits (864), Expect = 2.045e-97 Identity = 183/462 (39.61%), Postives = 271/462 (58.66%), Query Frame = 0
Query: 695 QSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKS-SMSLAILDK-DGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
+ Q F+T LK++Q+L +HVKNYRPK++V +G P RP L+DFA+L+TK+LSLL + FK ++ L++ W + IKSF+S+T+N + EGA A + + G+GK+ PNMVL G+K DW + +EY L AFD+ L VG+LR++ G D S++ +E+ Q EL E++ ED K G+ G L QKK + +S A+ DG P+ + TV+ ITQF+ K R G ID++WLYDDGGLT+LLP+ILTTR +FA+ +LR+F L+ DELD +TRNM LL+KFRI + V ++ D+TKKA T+++FK ++ P + E E+ E+TN LR+ E++R++S +++++MTL +P+R LYM+WL+ T+ MPPFL++RGNQ SVLT
Sbjct: 537 KXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHVNIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLAGIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKVIGEEEQQQ-------HEIELDEEN---ED--------------------EKNGKQGKSLELQQKKVRXRKVSTAVYTGIDGNPLPKNTVQDITQFQIKK-RKGNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKILDEIKPP------------------------LEESEIISQKEKTNRHLRLTELLREHSKRSEMIIMTLSIPRRGTAVAQLYMSWLEMMTKNMPPFLLIRGNQSSVLT 943
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000003697 (supercontig:LSalAtl2s:LSalAtl2s1:1949199:1954526:1 gene:EMLSAG00000003697 transcript:EMLSAT00000003697 description:"maker-LSalAtl2s1-augustus-gene-19.17") HSP 1 Score: 278.1 bits (710), Expect = 2.704e-78 Identity = 200/665 (30.08%), Postives = 337/665 (50.68%), Query Frame = 0
Query: 191 AEDSSEGKAIK-FGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMD-------MLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSS-----DEEIAKGFVG-----YDTAVLESNLYNAYT---KDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCE--GRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMD-FKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLN-SNH--IKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLAD 828
AED G+ GWF G + L+++ +LFLR+ +++G AG+L L I ++ + V T +S+SA++TNG +K GG+Y+MISR+LGPEFGG+IG++F AN + ++Y + E ++ L YL + + + R+ LI +L L++ ++G + + +L+ ++ + I S+ +E I+ F G + +S+LY +Y+ D+T V NF VFAV F VTGI+AGANLSGDLK+P+ +IPKGTLLA T++TY+I + +T D E R C+Y M+E S W P + G AT ++ + L+G+ RVL+A++KD +F + N+PV ++ +++S I IG +N ++ ++ F+ +YA +N S + S+P +RP F+ ++ + LG I + +MF+++ +A++ CI + K NWGS SQ+ F K + L DHVK +RP++++L NP + SL+DFA+ L K+ L + E + + + D WLN +H +K+F +T ++ G + + I G+G + PN ++ GF + + D
Sbjct: 11 AEDEHVGRGSGGLGWFKGXFAPVSLSMFSSLLFLRVGYILGNAGILHSLLQIVIAYGILVFTILSVSAIATNGAVKGGGVYFMISRTLGPEFGGSIGVLFYAANIASSALYAVACTECIISSFGEESGTLAHYLPE-SFWYQILYRV----LIN----LLNLSICLIGPNLFGKTTIMVLISVVFCTLTVITSFFSASTVEVHFNETISGSFTGILSKNWSIENFQSHLYPSYSLDCNDDTKPV--NFAIVFAVLFSGVTGIMAGANLSGDLKEPSKSIPKGTLLASFFTFITYVI---------------------------------LFTLTAMTCDKELLLRDCSY-------MIE-FSFWKPFVIIGTVLATFCASTSCLIGSSRVLEAISKDTMFGIFLEWITYGTVGNNPVLAVVVTWLLSNIFFFIGGVNTIAQFVAVLFLLSYASVNLSCLSLDLASAPNFRPIFRCFSWYTCALGLIGTLTMMFMINPVISALVLFACIFFFLSLSFLSPTKENWGSISQALLFHQVRKYLLLLDSRKDHVKFWRPQVLLLVSNPRNHCSLIDFANAL-KKGGLYVLGHVREGNFEGMEEDECGLLMPHWLNLVDHLKVKAFVELTVAPTVRSGIQQLIRISGIGAMKPNTIIFGFPYEDENMCKD 622 HSP 2 Score: 271.937 bits (694), Expect = 3.037e-76 Identity = 189/627 (30.14%), Postives = 320/627 (51.04%), Query Frame = 0
Query: 1197 GPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKR--------AMGIV--GFDPDLLRSNLWPDFQ--KNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFP-YIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMD-LGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLR-DNH--IKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGF 1806
G GW +G + P L+++ +LFLR+ +++G AG+L S I IA + T +S+SA+ TNG +KGGG+YFMISR+LGPEFGG++G++F A + ++Y + E ++ + S + +S R+L + +L L I IG L K +++L+ ++ + + F + E GI+ + + +S+L+P + N D + + VFAV F VTG++AGAN+SGDLK+P +IPKGTL A T+ +Y++ + A + +ELLL R+C+Y MI+ S W P + G AT ++ + L+G RV +AI+KD +F ++E G P+ ++ + ++ IG +N ++ ++ F+ +YA +N S ++P +RP F+ F+ + LG + +M +++ + +AL CI NWGS SQ+ F K L L+ +DHVK +RP+++++ NP + +L DFAN + K+ L L HV + + E D + HWL +H +KAF LT + G++ + +SG+G + N ++ GF
Sbjct: 19 GSGGLGWFKGXFAPVSLSMFSSLLFLRVGYILGNAGILHSLLQIVIAYGILVFTILSVSAIATNGAVKGGGVYFMISRTLGPEFGGSIGVLFYAANIASSALYAVACTECIISSFGEESGTLAHYLPESFWYQILYRVLIN---LLNLSICLIGPNLFGKTTIMVLISVVFCTLTVITSFFSASTVEVHFNETISGSFTGILSKNWSIENFQSHLYPSYSLDCNDDTKPVNFAIVFAVLFSGVTGIMAGANLSGDLKEPSKSIPKGTLLASFFTFITYVILFTLTAMTC-------DKELLL-------------RDCSY-------MIEF-SFWKPFVIIGTVLATFCASTSCLIGSSRVLEAISKDTMFGIFLEWITYG-TVGNNPVLAVVVTWLLSNIFFFIGGVNTIAQFVAVLFLLSYASVNLSCLSLDLASAPNFRPIFRCFSWYTCALGLIGTLTMMFMINPVISALVLFACIFFFLSLSFLSPTKENWGSISQALLFHQVRKYLLLLDSRKDHVKFWRPQVLLLVSNPRNHCSLIDFANAL-KKGGLYVLGHVREGNFEGMEEDECGLLMPHWLNLVDHLKVKAFVELTVAPTVRSGIQQLIRISGIGAMKPNTIIFGF 612
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000004234 (supercontig:LSalAtl2s:LSalAtl2s222:1210791:1213007:1 gene:EMLSAG00000004234 transcript:EMLSAT00000004234 description:"maker-LSalAtl2s222-snap-gene-12.7") HSP 1 Score: 217.624 bits (553), Expect = 6.899e-60 Identity = 172/614 (28.01%), Postives = 290/614 (47.23%), Query Frame = 0
Query: 256 SAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISL-VLILALAIVGMEWVTRVQMGLLLLLLVSQV--------------DFIIGSVIPSSDEEIAK------------------GFVGYDTAVLESNLY-NAYTKDE--TSGVQH--NFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQM-MEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKP-----KVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR-LSLLESIQITEASLDFKKVDALKSAANEWL---NSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQD 821
SA+S+NG ++ GG+Y+M+SR+LGPEFGGAIG +F AN ++ ++Y+ G E L+ + + R + + S S+ L L + +VG ++ + + + ++LV + DFI + P + +K G ++ +L N Y N Y K E SG NFF +F V F VTGI++GAN+ G+L P+ +IP+GT+ A T V Y+I ++ + C+ +LQ M M +++ P + G F AT S+++ +L+G R+L+A+AKD +F + +F +PV I + + + +++G LN ++ L S F+ +Y IN + + S+P +RP++KY++ + L+G + I +MF + +AI + V I+L I NWGS SQ+ F K + L + HVK +RP++++L NP SL++F + L K L +L + + +L+ D + WL + +K+F +T SL G + I G+G + PN VL+GFK D
Sbjct: 16 SALSSNGAVEGGGVYFMLSRTLGPEFGGAIGTLFFFANVVSSALYLAGCTEGLVSNFK-----------FLARGPWIEFLYSTSINSLNLFICLVGSKFFGKTSVLIFFMVLVCVLMTSVSFFLDYTITEDFIFNATDPLKCVQPSKNDTNQTPNCTLTSTGKFTGIGSWNETILVQNFYDNLYPKYEYDCSGSDKSLNFFIIFGVLFSGVTGIMSGANMCGELVSPSKSIPRGTISACGFTLVIYIILTVLTSL----------------------------------------TCSPTLLQHDCMYMNIVNVLPPAVTIGVFMATFSASLNNLIGXSRILEAIAKDVVFGPLFNFVLKGTYKENPVTAVISTWFVVELFLMMGSLNNIAQLCSVLFLLSYGTINLTCLFLHLASAPNFRPTYKYFSTYTCLIGLLGSIIMMFAV---SAIYSAVGILLCLSLILLLNLFSPIRHTNWGSISQALIFHQVRKYLLLLDQRKSHVKFWRPQMLLLVSNPRRNCSLIEFGNALKKSGLYVLGHVHVD--NLEEHDKDPTLNEIPHWLKLIDFLKVKAFVEVTIARSLRLGVDQLVRISGIGAMKPNTVLLGFKDD 573 HSP 2 Score: 214.927 bits (546), Expect = 5.054e-59 Identity = 163/592 (27.53%), Postives = 287/592 (48.48%), Query Frame = 0
Query: 1255 SAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTF--------------VPPSDEK-------------RAMGIVGFDPDLL----RSNLWPDFQKNVDG--EQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNP--DVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLR---DNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQE 1808
SA+ +NG ++GGG+YFM+SR+LGPEFGGA+G +F A ++ ++Y+ G E L+ ++F ++ I N + + + L+G F G V ++L+ VL+ S FL T V PS + GI ++ +L NL+P ++ + G + L F +F V F VTG+++GANM G+L P +IP+GT++A C + + + I + + + +P L +C Y + N + P + G F AT S++L +L+G R+ +AIAKD +F + +F + P+ + + + +++G LN ++ L S F+ +Y IN + ++P +RP++KYF+ + L+G I++M + + ++ + L + + +P NWGS SQ+ F K L L+ + HVK +RP+++++ NP +L +F N + K+S L L HV +++E D + HWL+ +KAF +T S GV V +SG+G + N +++GF++
Sbjct: 16 SALSSNGAVEGGGVYFMLSRTLGPEFGGAIGTLFFFANVVSSALYLAGCTEGLVSN-FKFLARGPWIEFLYSTSINSLNLF-----ICLVGSKFFGKTSVLIFFMVLVCVLMTSVSFFLDYTITEDFIFNATDPLKCVQPSKNDTNQTPNCTLTSTGKFTGIGSWNETILVQNFYDNLYPKYEYDCSGSDKSLNFFIIFGVLFSGVTGIMSGANMCGELVSPSKSIPRGTISA-----CGFTL-VIYIILTVLTSLTCSPTLL--------------QHDCMYMNIVN--------VLPPAVTIGVFMATFSASLNNLIGXSRILEAIAKDVVFGPLFNFVLKGTYKENPVTAVISTWFVVELFLMMGSLNNIAQLCSVLFLLSYGTINLTCLFLHLASAPNFRPTYKYFSTYTCLIGLLGSIIMMFAVSAIYSAVGILLCLSLILLLNLFSPIRHTNWGSISQALIFHQVRKYLLLLDQRKSHVKFWRPQMLLLVSNPRRNCSLIEFGNAL-KKSGLYVLGHVHVDNLEEHDKDPTLNEIPHWLKLIDFLKVKAFVEVTIARSLRLGVDQLVRISGIGAMKPNTVLLGFKD 572
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000007778 (supercontig:LSalAtl2s:LSalAtl2s455:327857:377250:-1 gene:EMLSAG00000007778 transcript:EMLSAT00000007778 description:"maker-LSalAtl2s455-augustus-gene-2.20") HSP 1 Score: 186.037 bits (471), Expect = 1.310e-47 Identity = 119/432 (27.55%), Postives = 208/432 (48.15%), Query Frame = 0
Query: 410 TSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWG--PLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVL----SGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLD--FKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYY 833
TS + +F + +FFP+ TGI+AG+N SGDL P IP GT++AI+TT Y+ L+ A V+ + D F +++G L + AW ++ G +T+ + + SL GAPR+LQA+AKD + P + F + A +PVR + +I + +LIG++++++ LLS FF+ Y +N + ++ +P WRP FKYY+ +SLLG +LCI VMF+ + A++ ++Y +I +R + WG + + A ++ L H KN+RP+I++L S V L A L L + + + + + A K + + + +K F + + + +G + GLG + PN V++G+ W++ D+ + +
Sbjct: 442 TSDIYTSFVILVGIFFPSCTGIMAGSNRSGDLAXPQKXIPIGTIMAILTTSTVYLSSVLLFA--------ATVDPLLL-RDKFGESIGGSLVVGNI------------------------AWPHPKVVEIGSLLSTIGAGLQSLTGAPRLLQAIAKDGIIPFLAPF-QVQSAKGEPVRALGITLLICEVAVLIGNVDEIAPLLSMFFLMCYGFVNLAXALQTLLRTPNWRPRFKYYHWSLSLLGVVLCIAVMFMSSWYYALLAMGIAGIIYKYIEYRGAEKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILAKLDSQLKVKHKRLFALASQLKAGKGLTIGVTVLDGHFGKMYGEAQAAKFSIQQVIKDEAVKGFADVVVSKDISDGVCHLTQTTGLGGMKPNTVILGWPNSWRKKEEDSWKVF 839 HSP 2 Score: 162.54 bits (410), Expect = 3.122e-40 Identity = 116/424 (27.36%), Postives = 192/424 (45.28%), Query Frame = 0
Query: 1421 AVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWG--PLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKED----VEPRIMDN-----------MKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLS 1827
+FFP+ TG++AG+N SGDL P IP GT+ AILTT Y+ L+ AA+ + LL D+ +G ++ + W ++ G +T+ + L SL G PR+ QAIAKD + P++ F + EP+R + I +LIG+++ ++ LLS FF+ Y +N + + +P WRP FKY++ +SLLG LCI VM + AL + ++Y ++ R + WG + + A SL L H KN+RP+++++ S L K L L +K + ++D K + ++D +K F + + GV + +GLG + N +++G+ W K E+ ++V V S
Sbjct: 454 GIFFPSCTGIMAGSNRSGDLAXPQKXIPIGTIMAILTTSTVYLSSVLLFAATVDPL-------LLRDK---------------FGESIGGSLVVGNIAWPHPKVVEIGSLLSTIGAGLQSLTGAPRLLQAIAKDGIIPFLAPFQVQSAKG-EPVRALGITLLICEVAVLIGNVDEIAPLLSMFFLMCYGFVNLAXALQTLLRTPNWRPRFKYYHWSLSLLGVVLCIAVMFMSSWYYALLAMGIAGIIYKYIEYRGAEKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILAKLDS---------QLKVKHKRLFALASQLKAGKGLTIGVTVLDGHFGKMYGEAQAAKFSIQQVIKDEAVKGFADVVVSKDISDGVCHLTQTTGLGGMKPNTVILGWPNSW---RKKEEDSWKVFVDS 842 HSP 3 Score: 139.428 bits (350), Expect = 3.895e-33 Identity = 84/202 (41.58%), Postives = 119/202 (58.91%), Query Frame = 0
Query: 1178 SNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFS-SQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV 1378
+N S T D K A G + GVY+PC+ NI+G +LF+R+TW+VG AG + F ++ + VT +TAISM+ + TNG + GGG FMISR+LGPEFGGAVG+ F A +A +MYIIG E L+ I + S F + + + N+ R+ G+ L+ + I FIG+ VNK + L +L S V +G FV
Sbjct: 124 ANYSATIQSXPTDPDAKPVQSANLGTLMGVYLPCIQNIFGXILFIRMTWIVGTAGAVEGFFVVFMGCCVTLLTAISMAXIATNGVVPGGGSXFMISRALGPEFGGAVGIXFYXAXXVAAAMYIIGAIEILIIYIVGPAMSLFGPDITNDFVKFNNYRVYGTFLLITMGTIVFIGVRFVNKFAAVALACVLLSIVSIYVGIFV 325 HSP 4 Score: 119.398 bits (298), Expect = 5.810e-27 Identity = 67/185 (36.22%), Postives = 110/185 (59.46%), Query Frame = 0
Query: 193 DSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNG--IVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIG 375
D+ ++ G GVY+ C+ NI+G +LF+R+TW++G AG +EG ++ + VT++T ISM+ ++TNG + GG +MISR+LGPEFGGA+GI F A +A +MYIIG E L+ + G I + + N+ R+ G+ L+ + + +G+ +V + L +L+S V +G
Sbjct: 138 DAKPVQSANLGTLMGVYLPCIQNIFGXILFIRMTWIVGTAGAVEGFFVVFMGCCVTLLTAISMAXIATNGVVPGGGSXFMISRALGPEFGGAVGIXFYXAXXVAAAMYIIGAIEILIIYIVGPAMSLFGPDITNDFVKFNNYRVYGTFLLITMGTIVFIGVRFVNKFAAVALACVLLSIVSIYVG 322 HSP 5 Score: 54.6842 bits (130), Expect = 2.949e-7 Identity = 47/205 (22.93%), Postives = 106/205 (51.71%), Query Frame = 0
Query: 961 GTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFS-DVILLQ--DMTKKADKKT-----RDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPK---REQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
G IDI+W+ DGGL MLLP +L + KLRIF ++++ D + +++ L RIE +V+ +Q D+++ ++T R++ + + + G++++ ++ V ++ ++++++ H+++ ++ + ++ + ++ + +P PK E+I ++M +L+ T + LMVRG V+T
Sbjct: 871 GNIDIWWIVHDGGLLMLLPFLLKQHRTWKNCKLRIFSVAQHEDNSIQMKKDLKTFLYHLRIEADVEVVEMQDSDISEYTYERTLLMEQRNEMLKEMRVSDKRKGKMTRRLSLNKGVGEYKVVDDIII-------HTDKVTNEIEVETVIENSRADVPNIEDKIP-PKVKFSEEID-EVHMEFLEVLTEGLERVLMVRGGGREVIT 1066
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709294|sp|P55013.1|S12A2_SQUAC (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; AltName: Full=NKCC; AltName: Full=Na-K-CL symporter) HSP 1 Score: 791.186 bits (2042), Expect = 0.000e+0 Identity = 418/1039 (40.23%), Postives = 660/1039 (63.52%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDEL---LDESVLSD--INGND---AEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQA----YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLL--ESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSL-ADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFE----DGSESKMSVDSGLASPATLTKRKLGRD---GSVLGRFQKKTKS-SMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +DHYR+ + ++ + RP+L EL LD+ D +NG + AE+ V+ ++G +KFGW GV +RC+LNIWGVMLF+RL+W++G AG+ L +I + +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGGAIG++F AN++AV+MY++GF E++ D+L ++ N ++ ++ ++D+R+IGS+++V++ +++ GMEW + Q+ LL +LL++ V+F +G+ IP++D+ AKGF Y + N + E +FFSVFA+FFPA TGI+AGAN+SGDL DP +AIPKGTLLAI+ T + Y + V C +R A+GN+ + + TV N+ + C KC+YG++ Q+M ++S +GPLI AG F+ATLSSA+ASLV AP++ QA+ KD ++PG+H FS G+G NN+P+RGY+L F I L ILI +LN ++ ++SNFF+A+YALINFSVFHAS+ SPGWRP+F++YN WISL+G ILC GVMF++++ A++T V ++ LY+++ ++KP VNWGSS+Q+ +++ AL+ LT V+DHVKN+RP+ ++++G P RP+L+ H TK + L+ + K++ ++ WL N +K+F++ + L EG + L+ GLG++ PN ++ GFK+DW+Q+L D E Y + AFD + V V+R+ G +IS + A +E + ++ + +N E D S+ S SPA +K D S +K+ K S+ L + D ++ ++ +QF+ K + GTID++WL+DDGGLT+L+P++LTT+ K+ K+R+ F+ ++ +D D R M LLSKFRI+FSD+ +L DM K K F+ MI + ++ + + EA +K E +T+ EL+ + +T Q+R+ E++R+NS A+L+VM+LP+ ++ + LYMAW++ ++ +PP L+VRGN SVLT
Sbjct: 189 TIDAVPRIDHYRH--TVAQLGEKLIRPSLAELHDELDKEPFEDGYVNGEESSPAEEAVSKHVADNKG-VVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGHAGIGLALLVIGTATVVTTITGLSTSAITTNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVRDLLVEH----NALM--IDEMSDIRIIGSVTIVVLFGISVAGMEWEAKAQIVLLGILLLAIVNFTVGTFIPANDKR-AKGFFNYRGEIFSENFVPDFRDGE------DFFSVFAIFFPAATGILAGANISGDLADPQLAIPKGTLLAILITTIVYAGAAVSVGSCIVREATGNLTDA-----IIPGTVTNCTNVACKLGFNFSSCATNKCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHVFSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFRFYNMWISLIGAILCCGVMFVINWWAALLTNVIVLALYIYVTYKKPDVNWGSSTQALTYLNALQHAIRLTGVEDHVKNFRPQCLLMTGAPTSRPALLHLVHAFTKNVGLVVCGHVHTGPRRQALKEISTDQAKYQRWLIKNKMKAFYAPVYAEDLREGTQFLLQAVGLGRMRPNTLVFGFKKDWRQALMKDVENYINAIHDAFDYQYGVVVIRLKEGFNISHLQAQEE----LCTSQEKSAHPKDIVVNLEHSDADSSKPSSKSVSETNSPAVCQDQKDEEDDGKASTQPLLKKEVKDPSVPLNMTD-------QKLLQASSQFQKKQGK-GTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRV-FIGGKINRIDHDRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMI-----EPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLIVMSLPVARKGAVSSALYMAWIETLSKDLPPILLVRGNHQSVLT 1188 HSP 2 Score: 711.449 bits (1835), Expect = 0.000e+0 Identity = 405/1003 (40.38%), Postives = 603/1003 (60.12%), Query Frame = 0
Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDR----------EDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRI-----MDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERW-WLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHA---------LSLDEFQPKSVQLSSKNVDSFGTSSTRN--NSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLS--NRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS-------GDEQGVQRPI-------VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RP+L EL +S A+ V + G +FGWI+GV V C+LNIWGVMLF+RL+W+VG AG+ + +I A +VT++T +S SA+ TNG ++GGG Y++ISRSLGPEFGGA+GL+F A ++AV+MY++GF E + D++ + ++ ++ D +D+RI+GS T+V+L GIS GM K Q++LL +LL + V+F +GTF+P +D KRA G + ++ N PDF+ DGE F VFA+FFPA TG++AGAN+SGDL DP AIPKGTL AIL T Y A+ + + +R A+GN + ++ + F +C+ C+YG + + Q++ L S +GPLI AG F+ATLSSALASLV P++FQA+ KD ++P + F G+G++ EPLRGY+L F I L ILI +LN ++ ++SNFF+A+YA+IN+SVFHAS SPGWRP+F+++N WISL+G LC VM +++ AL T + ++ LYI+V + PDVNWGSS+Q+ +++ AL+ L VEDHVKN+RP+ +++TG P+ RPAL + TK L+ HV PR + +A WL N +KAFYA + G + ++ GLG++ N L+ GF++ W K E Y + +FD V V+R++ G +IS + A E++ T SQ A +D L H+ S+ E +V K+ + G +ST+ EV N D++++ +QF+ K K GTIDV+WL+DDGGLTLL+PY+L+T+KK+ CK+RVF NR DH + MA+LL KFRI+F D+ +L D+ P K+ + F+ M+ EQ + +++ L + +T +R+ E+ +ENS + L+VM+LP+ R+ + LY+AW++ +S+ +PP L VRGN +SVLTFYS
Sbjct: 212 RPSLAELHDELDKEPFEDGYVNGEESSPAEEAVSKHVADNKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGHAGIGLALLVIGTATVVTTITGLSTSAITTNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVRDLLVEHNA----LMID---EMSDIRIIGSVTIVVLFGISVAGMEWEAKAQIVLLGILLLAIVNFTVGTFIPAND-KRAKGFFNYRGEIFSENFVPDFR---DGEDF--FSVFAIFFPAATGILAGANISGDLADPQLAIPKGTLLAILITTIVYAGAAVSVGSCIVREATGNLTDAIIPGTVTNCTNVACKLGFNFSSCATNKCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHVFSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFRFYNMWISLIGAILCCGVMFVINWWAALLTNVIVLALYIYVTYKKPDVNWGSSTQALTYLNALQHAIRLTGVEDHVKNFRPQCLLMTGAPTSRPALLHLVHAFTKNVGLVVCGHV---HTGPRRQALKEISTDQAKYQRWLIKNKMKAFYAPVYAEDLREGTQFLLQAVGLGRMRPNTLVFGFKKDWRQALMKDVENYINAIHDAFDYQYGVVVIRLKEGFNISHLQ-AQEELCT--SQEKSAHPKDIVVNLEHSDADSSKPSSKSVSETNSPAVCQDQKDEEDDGKASTQPLLKKEVKDPSVPLNMTDQKLLQASSQFQKKQGK-GTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDH---DRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMIEPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLIVMSLPVARKGAVSSALYMAWIETLSKDLPPILLVRGNHQSVLTFYS 1191
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|2500792|sp|Q25479.1|NKCL_MANSE (RecName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter; AltName: Full=Na-K-CL symporter) HSP 1 Score: 788.875 bits (2036), Expect = 0.000e+0 Identity = 423/1038 (40.75%), Postives = 656/1038 (63.20%), Query Frame = 0
Query: 138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKA--IKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLF--NDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLA-DAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFA--SDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSV-----DSGLASPATLTKRK---LGRDGSVLGRFQKKTKSSMSLAIL--DKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKF---DALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
LT+E LP +++YRN K RP+L EL + ++++ + EK N + S A IK GW GV++ CLLNIWGVMLFLRL+WV+ QAG+ L II +S IV VITT+SMSA+ TNG++K GGIYY+ISRSLGPEFG ++GI+F AN++A SM IGFC+SL D+LR NG+ + + INDVR++G+++L+++ + +GM+W ++ Q L+ +++ + VDF++G+++ P + EIAKGFVG +A N + + E + NFFSVFA+FFP+VTGI AGAN+SGDLKDPA AIPKGTLLA++ + V+Y + L ++R ASGN+ TDL N TV + +++ C YG+ S +M+++SAWGP IY GC+AATLS+A+ +L+ PR++QA+ D+++PG+ FS+ +G + +P RGY+L F +SL+ +LI DLN ++ L+SNF++A+YALINF FH ++ GWRP+F+YYN W+SL G ++C+ +M L+ + +++T LY+ +++R+P VNWGSS+Q+Q + TAL + AL R +HVKNY P+++VL+G P RP+L+D +L++K SL+ I++ L +K A + +EWL +++F S S GARA ++ G+G+L+PN++LMG+K DW A D Y+ L TAF+ RLAV ++R+ GGLD S + + ++E A ++ EL + ++S + + L++ TLT + + + +++ + M I+ G +++ + +++ F+ K SGT+D++WLYDDGGLT+LLP+I++ R+ +A KLRIF L+ + E++ + RNM LL+KFRI++S + ++QD+T P P++ +L E + KF A + +++ ELQ + +TN QLR+ E++ NS +A LVVM+LP+P++ I PLYMAWL+ +R +PP L VRGN SVLT
Sbjct: 67 LTREALPRMENYRNSKRALK------RPSLGELHGDHLITE----EDEKDQNHRDTKSPTPAVGIKLGWIQGVFIPCLLNIWGVMLFLRLSWVVSQAGIGLSLVIIAISAIVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFGASVGIIFAFANAVAASMNTIGFCDSLNDLLRS-----NGLKITEDPINDVRIVGTVALLVMCIICAIGMDWESKAQNFLIAIIVGAMVDFVVGTIMGPKDNSEIAKGFVGLSSATFVENFKSDFRFSEK--LDQNFFSVFAIFFPSVTGIQAGANISGDLKDPASAIPKGTLLALLISMVSYTLMVLFAGGGALRDASGNI------TDLLIVNGTVTDYSSVSLC---ALNNTCEYGLHNSYSVMQLMSAWGPFIYGGCWAATLSTALTNLLSVPRLIQALGVDRIYPGLIFFSKPYGRHGEPYRGYVLTFFVSLLFLLIADLNTIAPLISNFYLASYALINFCTFHRALVRPLGWRPTFRYYNMWLSLAGFLMCVAIMLLVHWVMSLVTFAIFFTLYLIVHYRRPDVNWGSSTQAQMYKTALSSAHALARTGEHVKNYWPQLLVLAGRPQARPALVDLGNLISKAGSLMIVGDISQEKLSYKVRSARARSDDEWLRGRKVRAFCSRVHGFSFEPGARALVQGSGVGRLAPNVLLMGYKSDWTTCPANDLVSYFNVLHTAFENRLAVAIVRVSGGLDYSAVVSEGAEEGAAGSLTATSSSGELRVRRDGLIMHADSDLDIRDTQPKHNLSNLLTLTTSRSFTISECKEKDKKKKERKPNDMHRQIVYNTASGLELSKFQLAQMSLFQKKQ-ESGTLDVWWLYDDGGLTILLPYIISQRSAWANCKLRIFALANRLHEMELEERNMANLLAKFRIDYSSLTMVQDITDP---------------PQPET-----KALFDETIKKFTEESASPDCRISDMELQTLAVKTNRQLRLRELLLANSKDARLVVMSLPMPRKGSISAPLYMAWLEMMSRDLPPMLFVRGNHTSVLT 1057 HSP 2 Score: 722.62 bits (1864), Expect = 0.000e+0 Identity = 399/964 (41.39%), Postives = 580/964 (60.17%), Query Frame = 0
Query: 1189 KDTVKKDTG---PA---RFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKR-AMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCS----NRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDI--SGAHEKVMTA--ASQASGASDEDRFSMLNHA---LSLDEFQPK---SVQLSSKNVDSFGTSSTRN-------------NSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSG--DEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
KD +DT PA + GWIQGV++PCLLNIWGVMLFLRL+WVV QAG+ S II I+ +V +T +SMSA+CTNG++KGGGIY++ISRSLGPEFG +VG++F A ++A SM IGFC++L D++ + +E NDVRI+G+ L+++ I IGM +K Q L+ +++ + VDF++GT + P D A G VG N DF+ + +Q F VFA+FFP+VTG+ AGAN+SGDLKDP SAIPKGTL A+L + SY + L +R ASGN +LL+ + + S N C YG + ++QL S WGP IY GC+AATLS+AL +L+ PR+ QA+ D+++P + F + +GR EP RGY+L F ++L +LI DLN ++ L+SNF++A+YA+IN+ FH + GWRP+F+Y+N W+SL G +C+ +M+L+ +L T LY+ V R PDVNWGSS+Q+Q + TAL S L +HVKNY P+L+V+ G P RPAL D NLI+K SL+ + + +E + ++ WLR ++AF + SF G R V+ SG+G+L+ N+L++G++ W P Y+ VL +F+ L+V ++RV GGLD S + GA E + A+ +SG R ++ HA L + + QPK S L+ SF S + + ++ Y G + + + +M+ F+ K Q+ GT+DV+WLYDDGGLT+LLPYI+S R +A CK+R+F L+NR + E MA+LL KFRI++ + ++ D+T P+ ET A F + +E +S++ L +T LR+ E+ NS + LVVM+LP+PR+ I LY+AWL+ +SR +PP L+VRGN SVLTFYS
Sbjct: 105 KDQNHRDTKSPTPAVGIKLGWIQGVFIPCLLNIWGVMLFLRLSWVVSQAGIGLSLVIIAISAIVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFGASVGIIFAFANAVAASMNTIGFCDSLNDLLRSNGLKI------TEDPINDVRIVGTVALLVMCIICAIGMDWESKAQNFLIAIIVGAMVDFVVGTIMGPKDNSEIAKGFVGLSSATFVENFKSDFRFSEKLDQ-NFFSVFAIFFPSVTGIQAGANISGDLKDPASAIPKGTLLALLISMVSYTLMVLFAGGGALRDASGNITDLLIVNGTVTDYSSVSLCALNNTCEYGLHNSYSVMQLMSAWGPFIYGGCWAATLSTALTNLLSVPRLIQALGVDRIYPGLIFFSKPYGRHGEPYRGYVLTFFVSLLFLLIADLNTIAPLISNFYLASYALINFCTFHRALVRPLGWRPTFRYYNMWLSLAGFLMCVAIMLLVHWVMSLVTFAIFFTLYLIVHYRRPDVNWGSSTQAQMYKTALSSAHALARTGEHVKNYWPQLLVLAGRPQARPALVDLGNLISKAGSLMIVGDISQEKLSYKVRSARARSDDEWLRGRKVRAFCSRVHGFSFEPGARALVQGSGVGRLAPNVLLMGYKSDWTTCPANDLVSYFNVLHTAFENRLAVAIVRVSGGLDYSAVVSEGAEEGAAGSLTATSSSGELRVRRDGLIMHADSDLDIRDTQPKHNLSNLLTLTTSRSFTISECKEKDKKKKERKPNDMHRQIVYNTASGLELSKFQLAQMSLFQ-KKQESGTLDVWWLYDDGGLTILLPYIISQRSAWANCKLRIFALANRLHEMELEERNMANLLAKFRIDYSSLTMVQDITDPPQPETKALFDETIKKFTEESASPDCRISDMELQTLAVKTNRQLRLRELLLANSKDARLVVMSLPMPRKGSISAPLYMAWLEMMSRDLPPMLFVRGNHTSVLTFYS 1060
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|341941994|sp|P55012.2|S12A2_MOUSE (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1) HSP 1 Score: 783.097 bits (2021), Expect = 0.000e+0 Identity = 417/1034 (40.33%), Postives = 666/1034 (64.41%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSS-----------EGKAI-KFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEI-LNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLL--ESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDW-QQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLN--FEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +DHYR+ + ++ + RP+L EL DE + E G E+S+ E K + KFGW GV +RC+LNIWGVMLF+RL+W++GQAG+ + +I ++ +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGGAIG++F AN++AV+MY++GF E+++++L+++ + ++ IND+R+IG+I++V++L +++ GMEW + Q+ LL++LL++ DF+IG+ I S + + KGF GY + + N + ++ET FFSVFA+FFPA TGI+AGAN+SGDL DP AIPKGTLLAI+ T V Y+ + V C +R A+GNV + T+L N T LN +Y CE C+YG++ + Q+M ++S + PLI AG F+ATLSSA+ASLV AP++ QA+ KD ++P F++G+G NN+P+RGYIL F+I+L ILI +LN ++ ++SNFF+A+YALINFSVFHAS+ SPGWRP+FKYYN WISL+G ILC VMF++++ A++T V ++ LY+++ ++KP VNWGSS+Q+ ++++AL+ L+ V+DHVKN+RP+ +V++G+P RP+L+ H TK + L+ + + K++ ++ WL N +K+F++ D L EGA+ ++ GLG++ PN +++GFK+DW Q + D + Y AFD++ V V+R+ GLDIS + +E + + + + +N + S+ SG S +T++ DG + K +S + L+ +++ +E TQF+ K ++ TID++WL+DDGGLT+L+P++LTT+ K+ K+R+ F+ ++ +D D R M LLSKFRI+FSD+++L D+ K K+ F MI P+ ++ + + + DK E +T+ EL+ + +T Q+R+ E+++++SS A+++VM+LP+ ++ + LYMAWL+ ++ +PP L+VRGN SVLT
Sbjct: 210 TMDAVPRIDHYRH--TAAQLGEKLLRPSLAELHDE-----LEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEH------SILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFI-SLESKKPKGFFGYKSEIFNENFGPDFREEET------FFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDT-ITTELTNCTSAACKLNFDFSY--CESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEE----LLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKS---ITQKDEEEDGKTPTQPLLKKESKGPIVPLNV----ADQKLLEASTQFQKKQGKN-TIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRV-FIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIE-PY----RLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLT 1202 HSP 2 Score: 725.702 bits (1872), Expect = 0.000e+0 Identity = 401/956 (41.95%), Postives = 602/956 (62.97%), Query Frame = 0
Query: 1197 GPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSS-QFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDR---------EDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPR--IMDNM---KAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYP-KFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSV---QLSSKNVDSFGTSSTRN--NSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLS--NRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS-------GDEQGVQRPI-------VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
G +FGWI+GV V C+LNIWGVMLF+RL+W+VGQAG+ S +I +A +VT++T +S SA+ TNG ++GGG Y++ISRSLGPEFGGA+GL+F A ++AV+MY++GF E +++++ + S D I ND+RI+G+ T+V+LLGIS GM K Q++LL++LL + DF+IGTF+ + K+ G G+ ++ N PDF+ E+ F VFA+FFPA TG++AGAN+SGDL DP SAIPKGTL AIL T Y+ A+ + + +R A+GN + + + F C + C+YG + N Q++ + S + PLI AG F+ATLSSALASLV P++FQA+ KD ++P + F +G+G++ EPLRGY+L F IAL ILI +LN ++ ++SNFF+A+YA+IN+SVFHAS SPGWRP+FKY+N WISL+G LC +VM +++ AL T + ++ LYI+V + PDVNWGSS+Q+ ++++AL+ L+ VEDHVKN+RP+ +V+TG+P+ RPAL + TK L+ HV + PR M M +A WL N +KAFYA D G + ++ +GLG++ N L++GF++ W + + Y + +FD+ V V+R++ GLDIS + G E + +SQ +D ++++ D+ KS ++ K+ + G + T+ E I N D++++ TQF+ K K TIDV+WL+DDGGLTLL+PY+L+T+KK+ CK+RVF NR DH + MA+LL KFRI+F D+++L D+ PKKE + F M+ EQ + + +++ L + +T +R+ E+ KE+S + ++VM+LP+ R+ + LY+AWL+ +S+ +PP L VRGN +SVLTFYS
Sbjct: 274 GVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEI--------NDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFIS-LESKKPKGFFGYKSEIFNENFGPDFR-----EEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHV---HMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELL---SSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKDEEEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGK-NTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDH---DRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLTFYS 1205
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709292|sp|P55011.1|S12A2_HUMAN (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1) HSP 1 Score: 782.326 bits (2019), Expect = 0.000e+0 Identity = 414/1039 (39.85%), Postives = 663/1039 (63.81%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGK------------AIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDT-VGEILNITQA-------YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLL--ESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDW-QQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +DHYR+ + ++ + RP+L EL DE + E G E+S+ + +KFGW GV +RC+LNIWGVMLF+RL+W++GQAG+ + +I ++ +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGGAIG++F AN++AV+MY++GF E+++++L+++ + ++ IND+R+IG+I++V++L +++ GMEW + Q+ LL++LL++ DF+IG+ IP ++ KGF GY + + N + ++ET FFSVFA+FFPA TGI+AGAN+SGDL DP AIPKGTLLAI+ T + Y+ + V C +R A+GNV NDT V E+ N T A + CE C+YG++ + Q+M ++S + PLI AG F+ATLSSA+ASLV AP++ QA+ KD ++P F++G+G NN+P+RGYIL F+I+L ILI +LN ++ ++SNFF+A+YALINFSVFHAS+ SPGWRP+FKYYN WISLLG ILC VMF++++ A++T V ++ LY+++ ++KP VNWGSS+Q+ +++ AL+ L+ V+DHVKN+RP+ +V++G P RP+L+ H TK + L+ + + K++ ++ WL N +K+F++ D L EGA+ ++ GLG++ PN +++GFK+DW Q + D + Y AFD++ V V+R+ GLDIS + +E + + + + ++ E +S + L S +T + DG + K +S + L+ +++ +E TQF+ K ++ TID++WL+DDGGLT+L+P++LTT+ K+ K+R+ F+ ++ +D D R M LLSKFRI+FSD+++L D+ K K+ F+ +I P+ ++ + + + DK E +T+ EL+ + +T Q+R+ E+++++SS A+++VM+LP+ ++ + LYMAWL+ ++ +PP L+VRGN SVLT
Sbjct: 217 TMDAVPRIDHYRH--TAAQLGEKLLRPSLAELHDE-----LEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEH------SILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKK-PKGFFGYKSEIFNENFGPDFREEET------FFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNV----------NDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEE----LLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPL-SEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNV----ADQKLLEASTQFQKKQGKN-TIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRV-FIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIE-PY----RLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLT 1209 HSP 2 Score: 728.013 bits (1878), Expect = 0.000e+0 Identity = 402/954 (42.14%), Postives = 606/954 (63.52%), Query Frame = 0
Query: 1197 GPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSS-QFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDR---------EDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPR--IMDNM---KAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYP-KFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQ-LSSKNVDSFGTSSTRN--NSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLS--NRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS-------GDEQGVQRPI-------VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
G +FGWI+GV V C+LNIWGVMLF+RL+W+VGQAG+ S +I +A +VT++T +S SA+ TNG ++GGG Y++ISRSLGPEFGGA+GL+F A ++AV+MY++GF E +++++ + S D I ND+RI+G+ T+V+LLGIS GM K Q++LL++LL + DF+IGTF+P + K+ G G+ ++ N PDF+ E+ F VFA+FFPA TG++AGAN+SGDL DP SAIPKGTL AIL T Y+ A+ + + +R A+GN + ++ + F +C + C+YG + N Q++ + S + PLI AG F+ATLSSALASLV P++FQA+ KD ++P + F +G+G++ EPLRGY+L F IAL ILI +LN ++ ++SNFF+A+YA+IN+SVFHAS SPGWRP+FKY+N WISLLG LC +VM +++ AL T + ++ LYI+V + PDVNWGSS+Q+ +++ AL+ L+ VEDHVKN+RP+ +V+TG P+ RPAL + TK L+ HV + PR M M +A WL N +KAFYA D G + ++ +GLG++ N L++GF++ W + + Y + +FD+ V V+R++ GLDIS + G E+++++ ++ G D + LD +P S + ++ K + G ++T+ E I N D++++ TQF+ K K TIDV+WL+DDGGLTLL+PY+L+T+KK+ CK+RVF NR DH + MA+LL KFRI+F D+++L D+ PKKE + F+ ++ EQ + + +++ L + +T +R+ E+ KE+S + ++VM+LP+ R+ + LY+AWL+ +S+ +PP L VRGN +SVLTFYS
Sbjct: 281 GVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEI--------NDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIP-LESKKPKGFFGYKSEIFNENFGPDFR-----EEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHV---HMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQG-QEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGK-NTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDH---DRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS 1212
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709297|sp|P55016.1|S12A1_RAT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 776.933 bits (2005), Expect = 0.000e+0 Identity = 411/1050 (39.14%), Postives = 662/1050 (63.05%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLS-DINGNDAEKGVNG----AEDSSEGK------AIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDS--VERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQA--------YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR--LSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQS-LADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGR----------PVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +++YRN S S P+ +RP+L E+ ++ + + A++ NG ++ +E K +KFGW GV +RC+LNIWGVMLF+RL+W++G+AG+ G+ II LS +VT +T ISMSA+ TNG ++ GG YY+ISRSLGPEFGG+IG++F AN++ V+MY++GF E+++D+L++ DS V+ ND+R+IGSI++V++L +++ GMEW + Q+ LL++LL+ +F IG+VIPS++E+ ++GF Y ++ N ++T+ E FFSVFA+FFPA TGI+AGAN+SGDL+DP AIP+GT+LAI T V Y+ + V C +R A+G++ NDTV +N + + C+ C YG++ + Q+M ++S +GPLI AG F+ATLSSA+ASLV AP+V QA+ KD +F G+ F++G+G NN+P+RGY L F+I++ ILI +LN ++ ++SNFF+A+YALINFS FHAS SPGWRP++ YN W+SL G ILC VMF++++ A+IT V + LY+++ ++KP VNWGSS+Q+ S+++AL LT V+DHVKN+RP+ +VL+G P+ RP+L+D H TK L + + + L K++++ + WL N IK+F++ D +G R+ L+ GLG++ PN +++G+K++W+++ L++ E Y + AFD + V ++RI G DIS + ++ + + + R E + + + E+G G + G F+K K +++ KD N++ VE QF+ K + GTID++WL+DDGGLT+L+P+ILT R K+ KLRI ++ ++ ++E+ +M +LLSKFRI+F+D+ ++ D+ K +K++ F+ MI P+ ++ +S +K +T+ EL+ E++ Q+R+ E+++++S A+L+V++LP+ ++ I LYMAWL+ T+ +PP L+VRGN +VLT
Sbjct: 101 TMDAVPKIEYYRNTGSVSG--PKVNRPSLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFRFANAVRVAMYVVGFAETVVDLLKE--------SDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIGIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTEGE------GFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVRACVVRDATGSM----------NDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKL-EQERLALEAAIKDNDCEEGK------------------------GGIRGLFKKAGKLNITKPAPKKDSNISTIQSMHVGEFNQKLVEASAQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIE-PY----RLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLT 1092 HSP 2 Score: 716.842 bits (1849), Expect = 0.000e+0 Identity = 397/1005 (39.50%), Postives = 612/1005 (60.90%), Query Frame = 0
Query: 1155 SRPTLDELLCR-SRNGSIYPCEGRSNQSG---TADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDRED---------ELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRI-----MDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGA--------HEKVMTAASQASGASDEDR---FSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV-------------SGDEQGVQRPI-VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+RP+L E+ + ++N ++ P +G D QA++ + TG +FGW++GV V C+LNIWGVMLF+RL+W+VG+AG+ II ++ +VT++T ISMSA+CTNG ++GGG Y++ISRSLGPEFGG++GL+F A ++ V+MY++GF E ++D++ + D ++ D ND+RI+GS T+V+LLGIS GM K Q++LL++LL +F IGT +P ++EK++ G + + N P F +GE F VFA+FFPA TG++AGAN+SGDL+DP AIP+GT+ AI T +Y+ A+ + A +R A+G+ + ++ + F C + C YG + N Q++ + S +GPLI AG F+ATLSSALASLV P+VFQA+ KD +F ++ F +G+G++ EPLRGY L F IA+ ILI +LN ++ ++SNFF+A+YA+IN+S FHAS+ SPGWRP++ +N W+SL G LC VM +++ A+ T + + LYI+V + PDVNWGSS+Q+ S+++AL + L VEDHVKN+RP+ IV+TG P RPAL D + TK S L V V PR M++ A WL N IKAFYA D F GVR+ ++ SGLG++ N L+IG+++ W P E Y ++ +FD + V ++R+ G DIS + E++ A+ +E + + A L+ +P + K + T + + E ++++V QF+ K K GTIDV+WL+DDGGLTLL+PYIL+ RKK+ CK+R+ ++ + + + EE MASLL KFRI+F D+ I+ D+ P KE+ F+ M+ + ++ + P +++ L +++ +R+ E+ +E+S + L+V++LP+ R+ I LY+AWL+ +++++PP L VRGN ++VLTFYS
Sbjct: 123 NRPSLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFRFANAVRVAMYVVGFAETVVDLL----KESDSMMVDP---TNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIGIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSF---TEGEGF--FSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVRACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVF---VGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKP-----APKKDSNISTIQSMHVGE----------FNQKLVEASAQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS 1095
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|27151793|sp|P55014.2|S12A1_MOUSE (RecName: Full=Solute carrier family 12 member 1; AltName: Full=BSC1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 774.622 bits (1999), Expect = 0.000e+0 Identity = 413/1050 (39.33%), Postives = 664/1050 (63.24%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLS-DINGNDAEKGVNG----AEDSSEGK------AIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDS--VERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQA--------YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR--LSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQS-LADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGR----------PVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +++YRN S S P+ +RP+L E+ ++ + + A++ NG ++ +E K +KFGW GV +RC+LNIWGVMLF+RL+W++G+AG+ G+ II LS +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGG+IG++F AN++AV+MY++GF E+++D+L++ DS V+ ND+R+IGSI++V++L +++ GMEW + Q+ LL++LL++ +F IG+VIPS++E+ ++GF Y ++ N ++TK E FFSVFA+FFPA TGI+AGAN+SGDL+DP AIP+GT+LAI T V Y+ + VA C +R A+G++ NDT+ +N + + C+ C YG++ + Q+M ++S +GPLI AG F+ATLSSA+ASLV AP+V QA+ KD +F G+ F++G+G NN+P+RGY L F+I++ ILI +LN ++ ++SNFF+A+YALINFS FHAS SPGWRP++ YN W+SL G ILC VMF++++ A+IT V + LY+++ ++KP VNWGSS+Q+ S+++AL LT V+DHVKN+RP+ +VL+G P+ RP+L+D H TK L + + + L K++++ + WL N IK+F++ D +G R+ L+ GLG++ PN +++G+K++W+++ L++ E Y + AFD + V ++RI G DIS + ++ + + + R E + + E+G G + G F+K K +++ KDG N++ VE QF+ K + GTID++WL+DDGGLT+L+P+ILT R K+ KLRI ++ + ++E+ +M +LLSKFRI+F+D+ ++ D+ K +K++ F+ MI P+ ++ +S +K +T+ EL+ E++ Q+R+ E+++++S A+L+V++LP+ ++ I LYMAWL+ T+ +PP L+VRGN +VLT
Sbjct: 101 TMDAVPKIEYYRNTGSVSG--PKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKE--------SDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGE------GFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSM----------NDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKL-EQERLALEAAIKDNECEEGK------------------------GGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIE-PY----RLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLT 1092 HSP 2 Score: 711.835 bits (1836), Expect = 0.000e+0 Identity = 397/1004 (39.54%), Postives = 613/1004 (61.06%), Query Frame = 0
Query: 1155 SRPTLDELLCR-SRNGSIYPCEGRSNQSG---TADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDRED---------ELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRI-----MDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGN----------PIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV-------------SGDEQGVQRPI-VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+RP+L E+ + ++N ++ P +G D QA++ + TG +FGW++GV V C+LNIWGVMLF+RL+W+VG+AG+ II ++ +VTS+T +S SA+ TNG ++GGG Y++ISRSLGPEFGG++GL+F A ++AV+MY++GF E ++D++ + D ++ D ND+RI+GS T+V+LLGIS GM K Q++LL++LL + +F IGT +P ++EK++ G + + N P F K GE F VFA+FFPA TG++AGAN+SGDL+DP AIP+GT+ AI T +Y+ A+ +AA +R A+G+ + ++ + F C + C YG + N Q++ + S +GPLI AG F+ATLSSALASLV P+VFQA+ KD +F ++ F +G+G++ EPLRGY L F IA+ ILI +LN ++ ++SNFF+A+YA+IN+S FHAS+ SPGWRP++ +N W+SL G LC VM +++ A+ T + + LYI+V + PDVNWGSS+Q+ S+++AL + L VEDHVKN+RP+ IV+TG P RPAL D + TK S L V V PR M++ A WL N IKAFYA D F GVR+ ++ SGLG++ N L+IG+++ W P E Y ++ +FD + V ++R+ G DIS + +++ +++R + L A+ +E + + + + N +DGN ++++V QF+ K K GTIDV+WL+DDGGLTLL+PYIL+ RKK+ CK+R+ ++ + + EE MASLL KFRI+F D+ I+ D+ P KE+ F+ M+ + ++ + P +++ L +++ +R+ E+ +E+S + L+V++LP+ R+ I LY+AWL+ +++++PP L VRGN ++VLTFYS
Sbjct: 123 NRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLL----KESDSMMVDP---TNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTK---GEGF--FSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVF---VGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKL--------EQERLA-LEAAIKDNE-----CEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS 1095
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|212276464|sp|Q13621.2|S12A1_HUMAN (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 770.385 bits (1988), Expect = 0.000e+0 Identity = 428/1104 (38.77%), Postives = 677/1104 (61.32%), Query Frame = 0
Query: 85 PSPYLPTRNGN--RKRSSVTIAPGTILEKPLPSQRKMTSKQRSSFILTPKPLKHYL-------TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDE---------SVLSDINGNDAEKGVNGAEDSSEGKA--IKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDS--VERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQA--------YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR--LSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQS-LADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMF-ASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
P Y T G+ +KR ++ PG Q T+K +SF Y T + +P +++YRN S S P+ +RP+L E+ ++ S + D G AE+ + +A +KFGW GV +RC+LNIWGVMLF+RL+W++G+AG+ G+ II LS +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGG+IG++F AN++AV+MY++GF E+++D+L++ DS V+ ND+R+IGSI++V++L +++ GMEW + Q+ LL++LL++ +F IG+VIPS++E+ ++GF Y ++ N +TK E FFSVFA+FFPA TGI+AGAN+SGDL+DP AIP+GT+LAI T V Y+ + V C +R A+GN+ NDT+ +N + + C C YG++ + Q+M ++S +GPLI AG F+ATLSSA+ASLV AP+V QA+ KD ++ + F++G+G NN+P+RGYIL F+I++ ILI +LN ++ ++SNFF+A+YALINFS FHAS SPGWRP++ YN W+SL G +LC VMF++++ A+IT V LYV++ +KP VNWGSS+Q+ S+++AL LT V+DHVKN+RP+ +VL+G P+ RP+L+D H TK L + + + L K++++ + WL N IK+F++ D +G R+ L+ GLG++ PN +++G+K++W+++ L + E Y + AFD + V ++RI G DIS++ +E ++ R + + + E G + +G +TK +DGS+ T SM + N++ VE TQF+ K GTID++WL+DDGGLT+L+P+ILT R K+ KLRI ++ ++ ++E+ M +LLSKFRI+F+D+ ++ D+ + +K++ F+ MI P+ ++ +S +K +T+ EL+ E++ Q+R+ E+++++S A+L+V++LP+ ++ I LYMAWL+ T+ +PP L+VRGN +VLT
Sbjct: 42 PPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISG--PKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKE--------SDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGE------GFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNM----------NDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAG---KLNITKTTPKKDGSI------NTSQSMHVG-------EFNQKLVEASTQFKKKQ-EKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIE-PY----RLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLT 1096 HSP 2 Score: 713.761 bits (1841), Expect = 0.000e+0 Identity = 397/1000 (39.70%), Postives = 611/1000 (61.10%), Query Frame = 0
Query: 1155 SRPTLDELLCR-SRNGSIYPCEGRSNQSGTA---DCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDRED---------ELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRI-----MDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDI-----SGAHEKVMTAASQASGASDE-DRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV-------------SGDEQGVQRPI-VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+RP+L E+ + ++N ++ P +G D QA++ G +FGW++GV V C+LNIWGVMLF+RL+W+VG+AG+ II ++ +VTS+T +S SA+ TNG ++GGG Y++ISRSLGPEFGG++GL+F A ++AV+MY++GF E ++D++ + D ++ D ND+RI+GS T+V+LLGIS GM K Q++LL++LL + +F IGT +P ++EK++ G + + N P F K GE F VFA+FFPA TG++AGAN+SGDL+DP AIP+GT+ AI T +Y+ A+ + A +R A+GN + ++ + F C + C YG + N Q++ + S +GPLI AG F+ATLSSALASLV P+VFQA+ KD ++ ++ F +G+G++ EPLRGY+L F IA+ ILI +LN ++ ++SNFF+A+YA+IN+S FHAS+ SPGWRP++ +N W+SL G LC VM +++ A+ T + LY++V + PDVNWGSS+Q+ S+++AL + L VEDHVKN+RP+ IV+TG P RPAL D + TK S L V V PR M++ A WL N IKAFYA D F GVR+ ++ SGLG++ N L+IG+++ W P E Y ++ +FD + V ++R+ G DIS + + A +A+ +E + S L + + + K S TS + + E ++++V TQF+ K +K GTIDV+WL+DDGGLTLL+PYIL+ RKK+ CK+R+ ++ + + + EE MASLL KFRI+F D+ I+ D+ P KE+ F+ M+ + ++ + P +++ L +++ +R+ E+ +E+S + L+V++LP+ R+ I LY+AWL+ +++++PP L VRGN ++VLTFYS
Sbjct: 127 NRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLL----KESDSMMVDP---TNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTK---GEGF--FSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVF---VGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGE----------FNQKLVEASTQFKKKQEK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS 1099
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709296|sp|P55015.1|S12A1_RABIT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 762.681 bits (1968), Expect = 0.000e+0 Identity = 408/1051 (38.82%), Postives = 658/1051 (62.61%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESV------------LSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDS--VERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQA--------YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR--LSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQS-LADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGR----------PVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +++YRN S S P+ +RP+L E+ ++ +++ G E+ E+ ++ A+KFGW GV +RC+LNIWGVMLF+RL+W++G+AG+ G+ II LS +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGG+IG++F AN++AV+MY++GF E+++D+L++ DS V+ ND+R+IGSI++V++L +++ GMEW + Q+ LL++LL++ +F IG+VIPS++E+ ++GF Y ++ N ++TK E FFSVFA+FFPA TGI+AGAN+SGDL+DP AIP+GT+LAI T V Y+ + V C +R A+G++ NDT+ +N + + C C YG++ + Q+M ++S +GPLI AG F+ATLSSA+ASLV A +V QA+ KD ++ + F++G+G NN+P+RGYIL F+I++ ILI +LN ++ ++SNFF+A+YALINFS FHAS SPGWRP++ YN W+SL G +LC VMF++++ A+IT V LY+++ ++KP VNWGSS+Q+ S+++AL LT V+DHVKN+RP+ VL+G P+ RP+L+D + TK L + + + L K++++ + WL N IK+F++ D +G R+ L+ GLG++ PN +++G+K+ W+++ L + E Y + AFD + V ++RI G DIS++ E+ + + + R E + + E+G +G + G F+K K +++ KD N++ VE TQF+ K + GTID++WL+DDGGLT+L+P+ILT R K+ KLRI ++ ++ ++E+ M +LLSKFRI+F+D+ ++ D+ K +K++ F+ MI P+ ++ +S +K +T+ EL+ E++ Q+R+ E+++++S A+L+V++LP+ ++ I LYMAWL+ T+ +PP L+VRGN +VLT
Sbjct: 104 TMDAVPKIEYYRNTGSVSG--PKVNRPSLLEIHEQLAKNVAVTPGSADRVANGEGMPGEEHAENKEEDNKAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVVIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKE--------SDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGE------GFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVGACVVRDATGSM----------NDTIISGINCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAAKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCFVLTGGPMTRPALLDITYAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKKWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELEKL-EQERLALEATIKDNECEEG------------------------NGGIRGLFKKVGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINIKPNKESWKFFEEMIE-PY----RLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLT 1096 HSP 2 Score: 699.893 bits (1805), Expect = 0.000e+0 Identity = 393/1006 (39.07%), Postives = 610/1006 (60.64%), Query Frame = 0
Query: 1155 SRPTLDELLCR-SRNGSIYP--CEGRSNQSGTADCQAKDTVKKDT--GPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDRED---------ELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRI-----MDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDI--------SGAHEKVMTAASQASGASDEDR---FSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV-------------SGDEQGVQRPI-VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+RP+L E+ + ++N ++ P + +N G + + ++D G +FGW++GV V C+LNIWGVMLF+RL+W+VG+AG+ II ++ +VTS+T +S SA+ TNG ++GGG Y++ISRSLGPEFGG++GL+F A ++AV+MY++GF E ++D++ + D ++ D ND+RI+GS T+V+LLGIS GM K Q++LL++LL + +F IGT +P ++EK++ G + + N P F K GE F VFA+FFPA TG++AGAN+SGDL+DP AIP+GT+ AI T +Y+ A+ + A +R A+G+ + ++ + F C + C YG + N Q++ + S +GPLI AG F+ATLSSALASLV +VFQA+ KD ++ ++ F +G+G++ EPLRGY+L F IA+ ILI +LN ++ ++SNFF+A+YA+IN+S FHAS+ SPGWRP++ +N W+SL G LC VM +++ A+ T + LYI+V + PDVNWGSS+Q+ S+++AL + L VEDHVKN+RP+ V+TG P RPAL D TK S L V V PR M++ A WL N IKAFYA D F GVR+ ++ SGLG++ N L+IG++++W P E Y ++ +FD + V ++R+ G DIS + E++ A+ +E + L+ +P + SS N T + + E ++++V TQF+ K K GTIDV+WL+DDGGLTLL+PYIL+ RKK+ CK+R+ ++ + + + EE MASLL KFRI+F D+ ++ D+ P KE+ F+ M+ + ++ + P +++ L +++ +R+ E+ +E+S + L+V++LP+ R+ I LY+AWL+ +++++PP L VRGN ++VLTFYS
Sbjct: 126 NRPSLLEIHEQLAKNVAVTPGSADRVANGEGMPGEEHAENKEEDNKAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVVIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLL----KESDSMMVDP---TNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTK---GEGF--FSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGINCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAAKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCFVLTGGPMTRPALLDITYAFTKNSGLCICCEVF---VGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKKWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELEKLEQERLALEATIKDNECEEGNGGIRGLFKKVGKLNITKPTPKKDSSIN-----TIQSMHVGE----------FNQKLVEASTQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINIKPNKESWKFFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS 1099
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|313104194|sp|P55017.3|S12A3_HUMAN (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl cotransporter; Short=NCC; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter) HSP 1 Score: 696.041 bits (1795), Expect = 0.000e+0 Identity = 384/1053 (36.47%), Postives = 613/1053 (58.21%), Query Frame = 0
Query: 137 YLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDEL---------------LDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEG---------------RKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLL--ESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSL-ADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEA-VDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKM-PPFLMVRGNQDSVLT 1154
Y T +V+P +HY N S G P + RPTL +L D ++ E + + + + ++FGW GV +RC+LNIWGV+L+LRL W+ QAG++ II LS VT IT +S+SA+STNG++K+GG Y++ISRSLGPE GG+IG++F AN++ V+M+ +GF E++ D+L++Y V+ IND+R+I +S+ ++LA+++ GMEW ++ Q+ L+++VS ++++G++IP S+++ +KGF Y + NL + G FF +F++FFP+ TGI+AGAN+SGDLKDPA+AIPKGTL+AI T ++Y+ + C +R ASG + NDTV T + CEG C+YG++ Q M ++S + PLI AG F ATLSSA+A LV A +V Q + +D+L+P I F +G+G N +PVRGY+L + I++ I+I +LN ++ ++SNFF+ +YALINFS FHASIT+SPGWRPSF+YYNKW +L G I+ + +MFL+ + A+I ++ L +++ ++KP+VNWGSS Q+ S+ AL L V+DH+KNYRP+ +VL+G P RP+L+DF T+ LSL+ + I ++ + + +WLN IK+F+S + L G + ++ GLG++ PN++++GFK++WQ + A E+Y L AFD V V+R+ GL++S+M + A EDG E+ VD P L K + + T F+++ + TIDIYWL+DDGGLT+L+P++L + +++K K+R+ F+ ++ +D++ + + +LLSKFR+ F +V +L D+ + + F+ MI G K EA V++ +++EE+ ++ ++ Q+R+ EIV S +A L+V+TLP+ ++ + P LYMAWL+ ++ + PP +++RGNQ++VLT
Sbjct: 58 YNTIDVVPTYEHYAN--STQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPI------VDPINDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDW-----RGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASG----------VLNDTV------TPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPA---------------EDGKEASARVD-----PKALVKEE------------------------------------QATTIFQSEQGKK-TIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRV-FVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDG------FKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLT 1017 HSP 2 Score: 655.596 bits (1690), Expect = 0.000e+0 Identity = 367/952 (38.55%), Postives = 569/952 (59.77%), Query Frame = 0
Query: 1193 KKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF-DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELL---------LDREDELYFQNCSNRN-CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMD-NMKAFVH-HWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWW-LYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAH-EKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS--------GDEQGVQR-----PI-VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSM-PPFLYVRGNQESVLTFY 2114
K P RFGW++GV + C+LNIWGV+L+LRL W+ QAG++ ++ II ++ VTS+T +S+SA+ TNG++K GG YF+ISRSLGPE GG++GL+F A ++ V+M+ +GF E + D++ ++ + D I ND+RI+ ++ +LL IS GM +K Q+L LV++ S ++L+GT +PPS++K + G + D+ NL PD++ G F +F++FFP+ TG++AGAN+SGDLKDP AIPKGTL AI T SY+ + I + +R ASG + + L F C+ ++ C YG + Q + + S + PLI AG F ATLSSALA LV +VFQ + +D+L+P I FG+G+G++KEP+RGYLL +AIA+ I+I +LN ++ ++SNFF+ +YA+IN+S FHAS NSPGWRPSF+Y+NKW +L G + +V+M L+ AL + ++ L ++V + P+VNWGSS Q+ S+ AL LN VEDH+KNYRP+ +V+TG P+ RPAL DF T+ SL+ HV+ + R+ + + A H WL IKAFY+ + GV+ ++ +GLG++ N+L++GF++ W +P E+Y +L +FD + V V+R++ GL++S + AH V A AS PK++ + E + T Q TID+YWL+DDGGLTLL+PY+L +++++KCK+RV F+ + + + +E + + SLL KFR+ F +V IL D+ ++P+ E F+ M++ DE V P +S+ + K+ ++ +R+ EI + S +AL+V+TLP+ R+ + P +LY+AWL+ +S+ + PP + +RGNQE+VLTFY
Sbjct: 128 KNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPI--------NDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWR----GPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEAS--------------ARVDPKAL------------------------------VKEE---QATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRV-FVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFY 1019
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1717802|sp|P55019.1|S12A3_PSEAM (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter) HSP 1 Score: 687.952 bits (1774), Expect = 0.000e+0 Identity = 396/1038 (38.15%), Postives = 608/1038 (58.57%), Query Frame = 0
Query: 137 YLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDEL-------------LDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDC-EGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQ-QSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITI----PHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKM-PPFLMVRGNQDSVLT 1154
Y T + PH D Y N + G R RP+L +L +E+ + G D+ + + +FGW GV +RC+LNIWGV+L+LRL W+ QAG+ II LS+ +T IT +S SA++TNG++K GG Y++ISRSLGPE GG+IG++F AN++AV+M+ +GF E++ D++R+ NG+V V+ IND+R++G I++ +L +++ GMEW ++ Q+ L+++VS V++I+G++IP+S ++ AKGF Y + +N + E S FF +F++FFP+ TGI+AGAN+SGDLKDP VAIP+GTL+AI T ++Y+I + C +R ASG + + + + G + +C + C +GI+ Q M ++SA+ PLI AG F ATLSSA+A LV AP+V Q + KD+L+P I F +G+G N +P+R Y+L ++I++ +LI +LN ++ ++SNFF+ +YALINFS FHAS+T+SPGWRPSF++Y+KW+SLLG + C+ +MFL+ + A+I + L + ++KP VNWGSS Q+ S+ AL L +V+DHVKNYRP+ +VL+G P RP+L+D LTKRLSL+ + A + WLN ++SF+ L G L+ GLG++ PN++LMGFK+DW S A Y L AFD+ V VLR+ GLD S + F+ G ES +V +PA + Q SS+S +D D +P + F+ K + TIDIYWL DDGGLT+LLP++LT R ++A K+R+ F+ + D+ +E + AL+ KFR+ F DV +L D+ + D F+ + +PK Q S S + + E ++TE++L+R+ ++ Q+R+ E+++ +S EA L+V+T+P+ +R P L++AWLD +R + PP L+VRGNQ++VLT
Sbjct: 57 YDTVDAPPHYDFYANTEVW--GRRRRVRPSLYQLSVDPEQDDFKPPMYEETAGERMGGGDSSEEEEEEHKEPPPEPPRFGWVQGVMIRCMLNIWGVILYLRLPWITAQAGIGLTWVIILLSSFITGITGLSTSAIATNGKVKGGGTYFLISRSLGPELGGSIGLIFAFANAVAVAMHTVGFAETVTDLMRE-----NGVV-MVDPINDIRIVGVITVTCLLGISMAGMEWESKAQVLFFLVIMVSFVNYIVGTIIPASPQKQAKGFFSYKAEIFAANFVPGWRGKEGS-----FFGMFSIFFPSATGILAGANISGDLKDPTVAIPRGTLMAIFWTTISYLIISATIGACVVRDASGELNDTLSYSSSSENCSGLACQYRWDFSECIKNNTCKHGIMNYYQSMSLVSAFAPLISAGIFGATLSSALACLVSAPKVFQCLCKDQLYPLIGFFGKGYGKNAEPLRAYLLTYVIAVCFVLIAELNTIAPIISNFFLCSYALINFSCFHASVTNSPGWRPSFRFYSKWLSLLGAVCCVVIMFLLTWWAALIAFGVVFFLLGYTLYKKPAVNWGSSVQASSYSMALNQCVGLNQVEDHVKNYRPQCLVLTGPPCCRPALVDLVGCLTKRLSLMMCGHVVTAG-----PSPVSERHVTWLNQRKVRSFYRGVVAADLRSGVNMLLQGAGLGRIKPNVLLMGFKKDWGCDSPQAAHHYIGILHDAFDLHYGVCVLRVKEGLDASH------------------PPQCHVNPGFDGGPESINTV----CAPACV---------------QTSVTSSVS---MDPDPQPS--------SVFQKKQGKK-TIDIYWLSDDGGLTLLLPYLLTRRKRWAGCKVRV-FVGGDTDKKEEQKEEVLALIKKFRLGFHDVEVLPDIHQPPQPGNVDHFEDSVNRFRLETNPK--QDSDSGPQQQQ-----QEEPWMITEQDLERNRAKSLRQIRLNEVLQVHSREAALIVITMPVGRRGVCPSTLFLAWLDVLSRDLRPPVLLVRGNQENVLT 1019 HSP 2 Score: 645.966 bits (1665), Expect = 0.000e+0 Identity = 373/946 (39.43%), Postives = 558/946 (58.99%), Query Frame = 0
Query: 1200 RFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELL-----------LDREDELYFQNC-SNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWL-YPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS----------GDEQGVQRP------IVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSM-PPFLYVRGNQESVLTFYS 2115
RFGW+QGV + C+LNIWGV+L+LRL W+ QAG+ ++ II +++ +T +T +S SA+ TNG++KGGG YF+ISRSLGPE GG++GL+F A ++AV+M+ +GF E + D++ + +G+V ND+RI+G T+ LLGIS GM +K Q+L LV++ S V++++GT +P S +K+A G + ++ +N P ++ G++ F +F++FFP+ TG++AGAN+SGDLKDP AIP+GTL AI T SY++ + I A +R ASG + L L + F C N C +G + Q + L S + PLI AG F ATLSSALA LV P+VFQ + KD+L+P I FG+G+G++ EPLR YLL + IA+ +LI +LN ++ ++SNFF+ +YA+IN+S FHAS NSPGWRPSF++++KW+SLLG C+V+M L+ AL + L + + P VNWGSS Q+ S+ AL LN VEDHVKNYRP+ +V+TG P RPAL D +TKR SL+ HV+ P ++ WL +++FY GV ++ +GLG++ N+L++GF++ W P+ Y +L +FD+H V VLRV+ GLD S H +N + P+S+ V + T S VS D +P + K Q TID+YWL DDGGLTLLLPY+L+ RK++A CK+RV F+ D E+ E++ +L+ KFR+ F DV +L D+ + P+ + +F+ V+ + G Q+ +++E +L ++ ++ +R+ E+ + +S +AL+V+T+P+ R+ P TL+LAWLD +SR + PP L VRGNQE+VLTFY
Sbjct: 132 RFGWVQGVMIRCMLNIWGVILYLRLPWITAQAGIGLTWVIILLSSFITGITGLSTSAIATNGKVKGGGTYFLISRSLGPELGGSIGLIFAFANAVAVAMHTVGFAETVTDLMRE-----NGVV--MVDPINDIRIVGVITVTCLLGISMAGMEWESKAQVLFFLVIMVSFVNYIVGTIIPASPQKQAKGFFSYKAEIFAANFVPGWR----GKEGSFFGMFSIFFPSATGILAGANISGDLKDPTVAIPRGTLMAIFWTTISYLIISATIGACVVRDASGELNDTLSYSSSSENCSGLACQYRWDFSECIKNNTCKHGIMNYYQSMSLVSAFAPLISAGIFGATLSSALACLVSAPKVFQCLCKDQLYPLIGFFGKGYGKNAEPLRAYLLTYVIAVCFVLIAELNTIAPIISNFFLCSYALINFSCFHASVTNSPGWRPSFRFYSKWLSLLGAVCCVVIMFLLTWWAALIAFGVVFFLLGYTLYKKPAVNWGSSVQASSYSMALNQCVGLNQVEDHVKNYRPQCLVLTGPPCCRPALVDLVGCLTKRLSLMMCGHVVTAGPSPVSERHVT-----WLNQRKVRSFYRGVVAADLRSGVNMLLQGAGLGRIKPNVLLMGFKKDWGCDSPQAAHHYIGILHDAFDLHYGVCVLRVKEGLDASHPPQCH---------------------VNPGF---DGGPESIN----TVCAPACVQTSVTSSVSM---DPDPQPSSVF-------QKKQGKKTIDIYWLSDDGGLTLLLPYLLTRRKRWAGCKVRV-FVGGDTDKKEEQKEEVLALIKKFRLGFHDVEVLPDIHQPPQPGNVDHFEDSVNRFRLETNPKQDSDSGPQQQQQEEPWMITEQDLERNRAKSLRQIRLNEVLQVHSREAALIVITMPVGRRGVCPSTLFLAWLDVLSRDLRPPVLLVRGNQENVLTFYC 1022
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1339058872|ref|XP_023717973.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Cryptotermes secundus]) HSP 1 Score: 1029.62 bits (2661), Expect = 0.000e+0 Identity = 538/1050 (51.24%), Postives = 715/1050 (68.10%), Query Frame = 0
Query: 138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEE---YYRTLETAFDMRLAVGVLRIDGGLDISEMFASDE----------KAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKD-------------------GRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
LT+EV P V+HYR+ S H + RPTLDEL + ++ +G D + A+ +GK IKFGW +GV+MRCLLNIWGVMLFLRL+WV+GQAG+ EG II L+NIVT++TTISMSAV TNGQIK GGIYYMISRSLGPEFGGAIG+MFTLANS+AVSMYI+GFCESL D+LR + T ++D ND+R++G ++L+ IL LAIVGM+WVTR QM LL +L+ SQ+DF++GS + P DEEIAKGFVGY++ SNL Y E GV H+FFSVFAVFFPAVTGIVAGANLSGDLKDP+ AIP GTLLAI T+++Y+ Y M+ C +R+ASGNV E + GE + + +C R C YG+ Q ME+ S+WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I FS+G GANNDPVRGY+LVF+IS CILI LN ++ LLSNFF+AAYALINFSVFHASI+ SPGWRP+FKYYN W+SL GT+LCI VMFLM + A+IT V +I LY+++ +RKP+VNWGSS+Q+ S+ +ALKA+ L RV++HVKN+RP+++VL+G P RP L+DFA+L+TK LSLL I + + + DAL A WL + +K+F +I +DS G++A ++I GLGKL PNM+LMG+K +W+ D EE Y+ + A D LAVG+LR+ GLD S + +E A+ RN +T++L Q +N S++ +S D+ + P T + G +K +SS ++ I K G P+ + + ITQF+ K + G ID++WLYDDGGL +L+P+IL+TR++F+ +LRIF L+ D+LD+D RN+ ALLSKFRI++SDV+++ D+ KKA + TR +F+ I K E + A+E +T+ EL E+TN +RI E++ Q S E+ +VM LP+P++ + PLYMAWL+ T+ MPP+L++RGNQ SV+T
Sbjct: 70 LTREVPPRVEHYRD---LSIQH--QYRPTLDELHERNIPEKSDGEDKVE----AQQQKKGKVIKFGWLEGVFMRCLLNIWGVMLFLRLSWVVGQAGIGEGTAIICLANIVTLVTTISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRTFGTT---LIDGAS--NDIRVVGLVTLIGILVLAIVGMDWVTRTQMCLLFVLIASQIDFMVGSFLGPQDDEEIAKGFVGYNSETFRSNLGPDYRNYE--GVDHDFFSVFAVFFPAVTGIVAGANLSGDLKDPSSAIPVGTLLAIFVTFLSYIGYAFMMGGCVLRTASGNVTE-------YLQVKGEP-DEWAVFSNCTDRSCLYGLHNDSQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSKGHGANNDPVRGYVLVFVISFGCILIAQLNAIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLAGTLLCIAVMFLMSWVMALITFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCADILKGTHSQRVHDALTRKAYHWLIRHKVKAFFNIVEDDSFEAGSQALMQISGLGKLRPNMILMGYKGNWRT--CDREELKQYFNVIHNALDRYLAVGILRVQEGLDYSGVIQDEEFVPISKLDKKDAEEKMRRNESTNQLESQDVNITVLSDA-LSSDTD-SPPVTPKMQHGGTVNCDPAATEKLLQSSTTVTITSKKMRKVSKNHKSSFPTYVGPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLALLIPYILSTRSQFSGCQLRIFSLASKRDDLDKDQRNLAALLSKFRIDYSDVVIISDIHKKAMESTRKEFEIFIQPFRVKD---------EENFETSKAIEQTYITDSELIALREKTNRHMRIRELLLQYSKESTFIVMALPIPRKGVVSAPLYMAWLELLTKGMPPYLIIRGNQTSVIT 1081 HSP 2 Score: 907.516 bits (2344), Expect = 0.000e+0 Identity = 488/1091 (44.73%), Postives = 685/1091 (62.79%), Query Frame = 0
Query: 1081 EEELQRHSEQ--TNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF-DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLL---DREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDIS--------------DISGAHEKVMTAASQASGASDEDRFSMLNHALSLDE----FQPKSVQLSSKNVDSFGT--------------------SSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS----GDEQ------GVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
++ELQ E T FQ+ I + + E D + + P + + Y +W T R++PP + + RPTLDEL RN P + A Q K V K FGW++GV++ CLLNIWGVMLFLRL+WVVGQAG+ AII +AN+VT VT ISMSAVCTNGQIKGGGIY+MISRSLGPEFGGA+GLMFT A S+AVSMYI+GFCE+L D++ F + DG +ND+R++G TL+ +L ++ +GM V + Q+ LL VL+ASQ+DF++G+F+ P DE+ A G VG++ + RSNL PD+ +N +G F VFAVFFPAVTG+VAGAN+SGDLKDP SAIP GTL AI T+ SY+ YA ++ +R ASGN E L + ++ F NC++R+C YG + Q ++L+S WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P+I F +G G + +P+RGY+L+F I+ CILI LN ++ LLSNFF+A YA+IN+SVFHAS + SPGWRP+FKY+N W+SL GT LCI VM LM AL T + +I LY++V R P+VNWGSS+Q+ S+ +ALK++ LN VE+HVKN+RP+++V+TG P RP L DFA LITK SLL ++K R+ D + +HWL + +KAF+ + +DSF G + +++SGLGKL NM+++G++ W + ++Y+ V+ + D +L+VG+LRVQ GLD S D A EK+ S S + ++L+ ALS D PK + N D T S +S +Y G P+ + I+ +TQF+ K K G IDV+WLYDDGGL LL+PYILSTR +F+ C++R+F L+++ D L ++ +A+LL KFRI++ DV+I+SD+ K + T F+ + DE+ +++ +++ L+ ++T H+RI E+ + S S +VM LP+PR+ + LY+AWL+ +++ MPP+L +RGNQ SV+T YS
Sbjct: 16 QQELQDIDENGSTRFQVDIVD-AENDDKENDYMTSSTPNQQYSYVYDSRYKSWGQLT-REVPPRVEHYRDLSIQHQYRPTLDEL--HERN---IPEKSDGEDKVEAQQQKKGKVIK------FGWLEGVFMRCLLNIWGVMLFLRLSWVVGQAGIGEGTAIICLANIVTLVTTISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRTFGTTLIDGA-------SNDIRVVGLVTLIGILVLAIVGMDWVTRTQMCLLFVLIASQIDFMVGSFLGPQDDEEIAKGFVGYNSETFRSNLGPDY-RNYEGVDHDFFSVFAVFFPAVTGIVAGANLSGDLKDPSSAIPVGTLLAIFVTFLSYIGYAFMMGGCVLRTASGNVTEYLQVKGEPDEWAVFSNCTDRSCLYGLHNDSQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSKGHGANNDPVRGYVLVFVISFGCILIAQLNAIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLAGTLLCIAVMFLMSWVMALITFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCADILKGTHSQRVHDALTRKAYHWLIRHKVKAFFNIVEDDSFEAGSQALMQISGLGKLRPNMILMGYKGNWRTCDREELKQYFNVIHNALDRYLAVGILRVQEGLDYSGVIQDEEFVPISKLDKKDAEEKMRRNESTNQLESQDVNITVLSDALSSDTDSPPVTPKMQHGGTVNCDPAATEKLLQSSTTVTITSKKMRKVSKNHKSSFPTYVGPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLALLIPYILSTRSQFSGCQLRIFSLASKRDDLDKDQRNLAALLSKFRIDYSDVVIISDIHKKAMESTRKEFEIFIQPFRVKDEENFETSKAIEQTYITDSELIALREKTNRHMRIRELLLQYSKESTFIVMALPIPRKGVVSAPLYMAWLELLTKGMPPYLIIRGNQTSVITVYS 1084
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1228359575|ref|XP_021952830.1| (solute carrier family 12 member 3-like [Folsomia candida]) HSP 1 Score: 1017.3 bits (2629), Expect = 0.000e+0 Identity = 529/1039 (50.91%), Postives = 713/1039 (68.62%), Query Frame = 0
Query: 138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYN----AYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQ-----AYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDW-QQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATL-----TKRKLGRDGSVLGRF------QKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
LT+E LP +D+YR+ LS RPTLDEL + + D E G + +GK IKFGW +GV MRC+LNIWGVMLFLRL+W +GQAG+LEG +I+L+N+VT IT SMSAVSTNGQIK GGIYYMISRSLGPEFG AIG+MFT+ANSIAVS+YI+GFCESL D+L+ ++ ++D ND+R+IG LV IL LA VGM+WVTR QM LL++L+ +QV+FIIGS I P D E AKGF GY+ +N ++ AY +D T + +F++VF VFFPAVTGIVAGANLSGDLKDPA AIPKGTLLAI TY++Y+ Y +M+ C++R ASGN+ E +N TV + + Q AYD+C R C YG+ S Q+ME+IS++G LIYAGCFAATLSSAIASLVGAPRV QA+ KDKL+P IH F++G+GANNDPVRGY LVF+ISL C+LI +LN ++ LLSNFF+ AY LINFSVFHASI+ SPGWRP+FKYYN W+SLLG +LCI VMFL+++ TA+ T C++ LY+++ +RKP+VNWGSS+Q+QSF +ALK++Q L V++HVKNYRP+I+V++G P +RP L+DFA+L+ K LSL+ + + K AL A WL + K F+++ ++S GA++ ++I GLGKL PNMVLMG+K DW + + D +EY+ + A DM LAVG+LR GLD S + +E+ Q+ + E+ ++ + SES++S D + ++ +RK D V + +K K S+S G + + ITQF+ K + GTID++WLYDDGGL++LLP++LTTR ++A LRIF L+ DEL + RNM ALLSKFRI++SDVI++ D+TKKA+ KT+ +F+++I + KS+ D + +TE EL H E+TN LR+ E+++ +S ++ VVMTLP+P++ + PLYMAWL+ TR MPPFL++RGNQ SVLT
Sbjct: 74 LTREALPALDNYRDLLSVHAA----PRPTLDELHNATYTEKTKAMDEETG-----EVLKGKVIKFGWLEGVLMRCMLNIWGVMLFLRLSWCVGQAGILEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTIANSIAVSLYIVGFCESLNDLLKA--NNWGQLIDGGS--NDIRVIGVGVLVAILVLAFVGMDWVTRTQMFLLVVLIAAQVNFIIGSFIGPVDDLEKAKGFPGYNAETFVTNFWSDYRTAYGEDTT---KQDFWTVFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYLSYIGYAVMMGGCAMREASGNLTE-------YNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVI--EEEGQLT---PQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPDQVVNNQLVGFSSNKKSRKHSLSAMYKGPGGAILPRHVLSNITQFQRKQ-KKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVI------------AEFKSD-----DNQDGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFVVMTLPMPRKGTVSAPLYMAWLEMLTRDMPPFLLLRGNQTSVLT 1066 HSP 2 Score: 895.19 bits (2312), Expect = 0.000e+0 Identity = 462/996 (46.39%), Postives = 655/996 (65.76%), Query Frame = 0
Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIY--QFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNV--DGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGN---------PEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLD-------------EFQPKSVQLSSKNVDSFGTSSTRNNSEVS----YGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV----SGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RPTLDEL + + Y + ++ T + +K FGW++GV + C+LNIWGVMLFLRL+W VGQAG+L F +I++ANLVT +TA SMSAV TNGQIKGGGIY+MISRSLGPEFG A+GLMFT A SIAVS+YI+GFCE+L D++ + DG +ND+R++G LV +L ++F+GM V + Q+ LL+VL+A+QV+F+IG+F+ P D+ ++A G G++ + +N W D++ D + + VF VFFPAVTG+VAGAN+SGDLKDP SAIPKGTL AI TY SY+ YA+++ MR ASGN E +L E + + NC+NR+C YG + Q+++L S +G LIYAGCFAATLSSA+ASLVG PRVFQA+ KDKL+P I F +G+G + +P+RGY L+F I+L C+LI +LN ++ LLSNFF+ Y +IN+SVFHAS ++SPGWRP+FKY+N W+SLLG LCI VM L++ TALAT+ C++ LY++V R P+VNWGSS+Q+QSF +ALKS+Q LN+VE+HVKNYRP+++V+TG P +RP L DFA LI K SL+ HV+K ++ + + WL + K FY L ++SF G ++ +++ GLGKL NM+++G++ W + +EY+ V+ + DMHL+VG+LR GLD S++ ++ Q E + LS D + + K++ N G SS + + + S Y G + ++ +TQF+ K QK GTIDV+WLYDDGGL++LLPY+L+TRK++A C +R+F L++R D L E MA+LL KFRI++ DVI++ D+TK + +T F++++ S D Q ++E L+ H ++T HLR+ E+ + +S S VVMTLP+PR+ + LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct: 96 RPTLDEL-----HNATYTEKTKAMDEETGEVLKGKVIK-------FGWLEGVLMRCMLNIWGVMLFLRLSWCVGQAGILEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTIANSIAVSLYIVGFCESLNDLLKANNWGQLIDG-------GSNDIRVIGVGVLVAILVLAFVGMDWVTRTQMFLLVVLIAAQVNFIIGSFIGPVDDLEKAKGFPGYNAETFVTNFWSDYRTAYGEDTTKQDFWTVFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYLSYIGYAVMMGGCAMREASGNLTEYNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVIEEEGQLTPQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPDQVVNNQLVGFSSNKKSRKHSLSAMYKGPGGAILPRHVLSNITQFQRK-QKKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVIAEFKSDDNQDGTS--ITESELMSHREKTNRHLRLRELLQLHSKDSTFVVMTLPMPRKGTVSAPLYMAWLEMLTRDMPPFLLLRGNQTSVLTFYS 1069
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1228009301|ref|XP_021936563.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X2 [Zootermopsis nevadensis]) HSP 1 Score: 1011.13 bits (2613), Expect = 0.000e+0 Identity = 543/1039 (52.26%), Postives = 710/1039 (68.33%), Query Frame = 0
Query: 138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEE---YYRTLETAFDMRLAVGVLRIDGGLDISEMFASDE----------KAQMIFARNRTTSELSEQSLNF--------EDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
LT+EV P V+HYR+ LS H E RPTL+EL E ++ + + E ++ +GK IKFGW +GV+MRCLLNIWGVMLFLRL+WVIGQAG+ EG II L+NIVT++T+ISMSAV TNGQIK GGIYYMISRSLGPEFGGAIG+MFTLANS+AVSMYI+GFCESL D+LR + T I+D ND+R++G ++L+ I LAIVGM+WVTR QM LL +L+ SQ+DF+IGS + P DEEI KGFVGY+ SNLY Y E GV H+FFSVF+VFFPAVTGIVAGANLSGDLKDP+ AIP GTLLAIV T+++Y+ YG M+ C +R+ASGNV E + GE N + +C R C YG+ Q ME+ S+WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I FS+G GANNDPVRGYILVF+IS CILI LN ++ LLSNFF+AAYALINFSVFHASI+ SPGWRP+FKYYN W+SL+GT+LCI VMFLM + A+ T V +I LY+++ +RKP+VNWGSS+Q+ S+ +ALKA+ L RV++HVKN+RP+++VL+G P RP L+DFA+L+TK LSLL I +A+ + +AL A +WL + +K+F IT +DS G + ++I GLGKL PNM+LMG+K +W+ D EE Y+ + A D LAVG+LR+ GLD S + +E A+ RN+++++L Q +N D M+ D +AS + L S R KT+ S + G P+ + + ITQF+ K + G ID++WLYDDGGLT+L+P+IL+TR++F+ +LRIF L+ D+LD D RN+ ALLSKFRI++SDV+++ D+ KKA TR +F+ I K G S +S S +TE EL E+TN +RI E++ Q S E+ +VM LP+P+++ + PLYMAWL+ T+ MPP L++RGNQ SV+T
Sbjct: 65 LTREVPPRVEHYRH-LSI---HHEY-RPTLEEL-HERIIPEKRERNVEAE---PQEQKKGKVIKFGWLEGVFMRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRSFGTT---IIDGAS--NDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNLYPDYRNYE--GVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTE-------YLQVKGEP-NEWTIFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRT--CDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDP-VASDSLLESSTTVTVTSKKSRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTS---------NKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGMPPCLIIRGNQTSVIT 1066 HSP 2 Score: 880.937 bits (2275), Expect = 0.000e+0 Identity = 474/1026 (46.20%), Postives = 661/1026 (64.42%), Query Frame = 0
Query: 1136 TRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF-DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLL---DREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLY-PKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDIS--------------DISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPK----------------SVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS------GDEQGV----QRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
TR++PP + + RPTL+EL R I P + N Q K V K FGW++GV++ CLLNIWGVMLFLRL+WV+GQAG+ AII +AN+VT VT+ISMSAVCTNGQIKGGGIY+MISRSLGPEFGGA+GLMFT A S+AVSMYI+GFCE+L D++ F + DG +ND+R++G TL+ + ++ +GM V + Q+ LL VL+ASQ+DF+IG+F+ P DE+ G VG++ + SNL+PD+ +N +G F VF+VFFPAVTG+VAGAN+SGDLKDP SAIP GTL AI+ T+ SY+ Y ++ +R ASGN E L + + F NC++R C YG + Q ++L+S WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P I F +G G + +P+RGY+L+F I+ CILI LN ++ LLSNFF+A YA+IN+SVFHAS + SPGWRP+FKY+N W+SL+GT LCI VM LM AL T + +I LY++V R P+VNWGSS+Q+ S+ +ALK++ LN VE+HVKN+RP+++V+TG P RP L DFA LITK SLL H++K R+ + + + WL + +KAF+ +T +DSF G + +++SGLGKL NM+++G++ W P+ ++Y+ V+ + D +L+VG+LRVQ GLD S D A EK+ S S + S+L+ AL+ D P +V ++SK S T T+ ++ Y G P+ + I+ +TQF+ K K G IDV+WLYDDGGLTLL+PYILSTR +F+ C++R+F L+++ D L + +A+LL KFRI++ DV+I+SD+ K T F+T + GD + ++E LL ++T H+RI E+ + S S +VM LP+PR++ + LY+AWL+ +++ MPP L +RGNQ SV+T YS
Sbjct: 66 TREVPPRVEHYRHLSIHHEYRPTLEELHER-----IIPEKRERNVEAEPQEQKKGKVIK------FGWLEGVFMRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRSFGTTIIDG-------ASNDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNLYPDY-RNYEGVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTEYLQVKGEPNEWTIFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRTCDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDPVASDSLLESSTTVTVTSKK--SRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGMPPCLIIRGNQTSVITVYS 1069
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1228009299|ref|XP_021936562.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X1 [Zootermopsis nevadensis]) HSP 1 Score: 1010.75 bits (2612), Expect = 0.000e+0 Identity = 543/1039 (52.26%), Postives = 710/1039 (68.33%), Query Frame = 0
Query: 138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEE---YYRTLETAFDMRLAVGVLRIDGGLDISEMFASDE----------KAQMIFARNRTTSELSEQSLNF--------EDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
LT+EV P V+HYR+ LS H E RPTL+EL E ++ + + E ++ +GK IKFGW +GV+MRCLLNIWGVMLFLRL+WVIGQAG+ EG II L+NIVT++T+ISMSAV TNGQIK GGIYYMISRSLGPEFGGAIG+MFTLANS+AVSMYI+GFCESL D+LR + T I+D ND+R++G ++L+ I LAIVGM+WVTR QM LL +L+ SQ+DF+IGS + P DEEI KGFVGY+ SNLY Y E GV H+FFSVF+VFFPAVTGIVAGANLSGDLKDP+ AIP GTLLAIV T+++Y+ YG M+ C +R+ASGNV E + GE N + +C R C YG+ Q ME+ S+WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I FS+G GANNDPVRGYILVF+IS CILI LN ++ LLSNFF+AAYALINFSVFHASI+ SPGWRP+FKYYN W+SL+GT+LCI VMFLM + A+ T V +I LY+++ +RKP+VNWGSS+Q+ S+ +ALKA+ L RV++HVKN+RP+++VL+G P RP L+DFA+L+TK LSLL I +A+ + +AL A +WL + +K+F IT +DS G + ++I GLGKL PNM+LMG+K +W+ D EE Y+ + A D LAVG+LR+ GLD S + +E A+ RN+++++L Q +N D M+ D +AS + L S R KT+ S + G P+ + + ITQF+ K + G ID++WLYDDGGLT+L+P+IL+TR++F+ +LRIF L+ D+LD D RN+ ALLSKFRI++SDV+++ D+ KKA TR +F+ I K G S +S S +TE EL E+TN +RI E++ Q S E+ +VM LP+P+++ + PLYMAWL+ T+ MPP L++RGNQ SV+T
Sbjct: 68 LTREVPPRVEHYRH-LSI---HHEY-RPTLEEL-HERIIPEKRERNVEAE---PQEQKKGKVIKFGWLEGVFMRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRSFGTT---IIDGAS--NDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNLYPDYRNYE--GVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTE-------YLQVKGEP-NEWTIFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRT--CDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDP-VASDSLLESSTTVTVTSKKSRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTS---------NKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGMPPCLIIRGNQTSVIT 1069 HSP 2 Score: 881.322 bits (2276), Expect = 0.000e+0 Identity = 485/1082 (44.82%), Postives = 685/1082 (63.31%), Query Frame = 0
Query: 1081 EEELQRHSEQTNFQLRIAEI-VRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF-DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLL---DREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLY-PKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDIS--------------DISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPK----------------SVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS------GDEQGV----QRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
++ELQ + + + R+A++ + + E D +T P + Y + L TR++PP + + RPTL+EL R I P + N Q K V K FGW++GV++ CLLNIWGVMLFLRL+WV+GQAG+ AII +AN+VT VT+ISMSAVCTNGQIKGGGIY+MISRSLGPEFGGA+GLMFT A S+AVSMYI+GFCE+L D++ F + DG +ND+R++G TL+ + ++ +GM V + Q+ LL VL+ASQ+DF+IG+F+ P DE+ G VG++ + SNL+PD+ +N +G F VF+VFFPAVTG+VAGAN+SGDLKDP SAIP GTL AI+ T+ SY+ Y ++ +R ASGN E L + + F NC++R C YG + Q ++L+S WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P I F +G G + +P+RGY+L+F I+ CILI LN ++ LLSNFF+A YA+IN+SVFHAS + SPGWRP+FKY+N W+SL+GT LCI VM LM AL T + +I LY++V R P+VNWGSS+Q+ S+ +ALK++ LN VE+HVKN+RP+++V+TG P RP L DFA LITK SLL H++K R+ + + + WL + +KAF+ +T +DSF G + +++SGLGKL NM+++G++ W P+ ++Y+ V+ + D +L+VG+LRVQ GLD S D A EK+ S S + S+L+ AL+ D P +V ++SK S T T+ ++ Y G P+ + I+ +TQF+ K K G IDV+WLYDDGGLTLL+PYILSTR +F+ C++R+F L+++ D L + +A+LL KFRI++ DV+I+SD+ K T F+T + GD + ++E LL ++T H+RI E+ + S S +VM LP+PR++ + LY+AWL+ +++ MPP L +RGNQ SV+T YS
Sbjct: 16 QQELQDIDQNSPNRFRVAKVDAKDDEKETD--SLTSPNLQHSYGCDSRYKS-LGQLTREVPPRVEHYRHLSIHHEYRPTLEELHER-----IIPEKRERNVEAEPQEQKKGKVIK------FGWLEGVFMRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRSFGTTIIDG-------ASNDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNLYPDY-RNYEGVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTEYLQVKGEPNEWTIFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRTCDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDPVASDSLLESSTTVTVTSKK--SRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGMPPCLIIRGNQTSVITVYS 1072
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1215275570|gb|OXA61879.1| (Bumetanide-sensitive sodium-(potassium)-chloride cotransporter [Folsomia candida]) HSP 1 Score: 1008.82 bits (2607), Expect = 0.000e+0 Identity = 529/1048 (50.48%), Postives = 713/1048 (68.03%), Query Frame = 0
Query: 138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIW---------GVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYN----AYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQ-----AYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDW-QQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATL-----TKRKLGRDGSVLGRF------QKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
LT+E LP +D+YR+ LS RPTLDEL + + D E G + +GK IKFGW +GV MRC+LNIW GVMLFLRL+W +GQAG+LEG +I+L+N+VT IT SMSAVSTNGQIK GGIYYMISRSLGPEFG AIG+MFT+ANSIAVS+YI+GFCESL D+L+ ++ ++D ND+R+IG LV IL LA VGM+WVTR QM LL++L+ +QV+FIIGS I P D E AKGF GY+ +N ++ AY +D T + +F++VF VFFPAVTGIVAGANLSGDLKDPA AIPKGTLLAI TY++Y+ Y +M+ C++R ASGN+ E +N TV + + Q AYD+C R C YG+ S Q+ME+IS++G LIYAGCFAATLSSAIASLVGAPRV QA+ KDKL+P IH F++G+GANNDPVRGY LVF+ISL C+LI +LN ++ LLSNFF+ AY LINFSVFHASI+ SPGWRP+FKYYN W+SLLG +LCI VMFL+++ TA+ T C++ LY+++ +RKP+VNWGSS+Q+QSF +ALK++Q L V++HVKNYRP+I+V++G P +RP L+DFA+L+ K LSL+ + + K AL A WL + K F+++ ++S GA++ ++I GLGKL PNMVLMG+K DW + + D +EY+ + A DM LAVG+LR GLD S + +E+ Q+ + E+ ++ + SES++S D + ++ +RK D V + +K K S+S G + + ITQF+ K + GTID++WLYDDGGL++LLP++LTTR ++A LRIF L+ DEL + RNM ALLSKFRI++SDVI++ D+TKKA+ KT+ +F+++I + KS+ D + +TE EL H E+TN LR+ E+++ +S ++ VVMTLP+P++ + PLYMAWL+ TR MPPFL++RGNQ SVLT
Sbjct: 74 LTREALPALDNYRDLLSVHAA----PRPTLDELHNATYTEKTKAMDEETG-----EVLKGKVIKFGWLEGVLMRCMLNIWQVNANFENEGVMLFLRLSWCVGQAGILEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTIANSIAVSLYIVGFCESLNDLLKA--NNWGQLIDGGS--NDIRVIGVGVLVAILVLAFVGMDWVTRTQMFLLVVLIAAQVNFIIGSFIGPVDDLEKAKGFPGYNAETFVTNFWSDYRTAYGEDTT---KQDFWTVFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYLSYIGYAVMMGGCAMREASGNLTE-------YNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVI--EEEGQLT---PQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPDQVVNNQLVGFSSNKKSRKHSLSAMYKGPGGAILPRHVLSNITQFQRKQ-KKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVI------------AEFKSD-----DNQDGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFVVMTLPMPRKGTVSAPLYMAWLEMLTRDMPPFLLLRGNQTSVLT 1075 HSP 2 Score: 887.1 bits (2291), Expect = 0.000e+0 Identity = 462/1005 (45.97%), Postives = 655/1005 (65.17%), Query Frame = 0
Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIW---------GVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIY--QFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNV--DGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGN---------PEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLD-------------EFQPKSVQLSSKNVDSFGTSSTRNNSEVS----YGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV----SGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RPTLDEL + + Y + ++ T + +K FGW++GV + C+LNIW GVMLFLRL+W VGQAG+L F +I++ANLVT +TA SMSAV TNGQIKGGGIY+MISRSLGPEFG A+GLMFT A SIAVS+YI+GFCE+L D++ + DG +ND+R++G LV +L ++F+GM V + Q+ LL+VL+A+QV+F+IG+F+ P D+ ++A G G++ + +N W D++ D + + VF VFFPAVTG+VAGAN+SGDLKDP SAIPKGTL AI TY SY+ YA+++ MR ASGN E +L E + + NC+NR+C YG + Q+++L S +G LIYAGCFAATLSSA+ASLVG PRVFQA+ KDKL+P I F +G+G + +P+RGY L+F I+L C+LI +LN ++ LLSNFF+ Y +IN+SVFHAS ++SPGWRP+FKY+N W+SLLG LCI VM L++ TALAT+ C++ LY++V R P+VNWGSS+Q+QSF +ALKS+Q LN+VE+HVKNYRP+++V+TG P +RP L DFA LI K SL+ HV+K ++ + + WL + K FY L ++SF G ++ +++ GLGKL NM+++G++ W + +EY+ V+ + DMHL+VG+LR GLD S++ ++ Q E + LS D + + K++ N G SS + + + S Y G + ++ +TQF+ K QK GTIDV+WLYDDGGL++LLPY+L+TRK++A C +R+F L++R D L E MA+LL KFRI++ DVI++ D+TK + +T F++++ S D Q ++E L+ H ++T HLR+ E+ + +S S VVMTLP+PR+ + LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct: 96 RPTLDEL-----HNATYTEKTKAMDEETGEVLKGKVIK-------FGWLEGVLMRCMLNIWQVNANFENEGVMLFLRLSWCVGQAGILEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTIANSIAVSLYIVGFCESLNDLLKANNWGQLIDG-------GSNDIRVIGVGVLVAILVLAFVGMDWVTRTQMFLLVVLIAAQVNFIIGSFIGPVDDLEKAKGFPGYNAETFVTNFWSDYRTAYGEDTTKQDFWTVFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYLSYIGYAVMMGGCAMREASGNLTEYNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVIEEEGQLTPQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPDQVVNNQLVGFSSNKKSRKHSLSAMYKGPGGAILPRHVLSNITQFQRK-QKKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVIAEFKSDDNQ--DGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFVVMTLPMPRKGTVSAPLYMAWLEMLTRDMPPFLLLRGNQTSVLTFYS 1078
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1003342407|gb|AMO44056.1| (Na+/K+/2Cl- cotransporter protein [Marsupenaeus japonicus]) HSP 1 Score: 997.268 bits (2577), Expect = 0.000e+0 Identity = 531/1041 (51.01%), Postives = 710/1041 (68.20%), Query Frame = 0
Query: 132 KPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNV---EEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLN--------FEDGSESKMSVDSG-----LASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
K L+HYLT+E LP HYRN S G RPTLD+L + + A+ + GK IKFGW +GV MRCLLNIWGVMLFLR++W++GQAGLL L II ++NIVTV+TT+SMSAV+TNG+IK GG+YYMISRSLGPEFGG+IG+MFTLANSIA + YIIGFC+SL D+L+ Y + IVD IND R++G+I+L+ +LALAIVGM+WVTRVQMGLL LL+ SQ+DF+IG + P + E ++GFVG + V N + + + GV + FF VF VFFPAVTGIVAGANLSGDLKDP AIPKGTLLAI T+VTY++Y M+A +R ASGNV E T+L + V + T D G C +G+ S Q+ME++SAWGPLIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+PGI+SFS G+GANNDPVRGYILVF+IS ICI+IGDLN VS+LLSNFF+AAY+LINFS FHAS+ SPGWRPSF YYN W+SL+G ILC+ VMFL+D+KTA+IT + I+ LY+F+ +RKP VNWGSS+Q+Q++++ALK+ L V++HVKNYRP+++VL+G P RP L+DFA +TK +SLL + + + ++L A WL + I++F+SI ++L EG+R ++ GLGKL PN+VL+G+K +W + + Y+ TL A DM V +LR+ GLD S++ DE ++ N TT + + +DG+ S+ S G A A +T G + K ++S++ G + ++ + IT FR K + GTID++WLYDDGGLT+LLP+ILTTR++++ LR+F L+ DELD + R+M LL+KFRI++SDVI++ D+ KKA + +R +F +I KS + +DK + E + ++E EL E+TN +R+ E++ +NS EA LVVMTLP+P++ + PLYMAWL+ TR MPPFL++RGNQ SVLT
Sbjct: 49 KSLRHYLTREALPKESHYRNLNSIVDGG--NVRPTLDDLHNNTFREQQQSTGADPEAQAT--NLRGKVIKFGWIEGVLMRCLLNIWGVMLFLRVSWMVGQAGLLYSLMIIIMANIVTVVTTLSMSAVATNGRIKGGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLQDLLKYYFNNAQ-IVDGA--INDTRIVGTITLIAVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVIGFFMGPLDNLERSQGFVGMSSGVFSHNFASDFRYFD--GVHYEFFGVFGVFFPAVTGIVAGANLSGDLKDPGSAIPKGTLLAIGITFVTYIVYPFMLAGSVVRDASGNVTMFEAYVNSTELLENPVFTNESCTT---DGGGGTCEFGLQNSFQVMELVSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIYSFSRGYGANNDPVRGYILVFVISFICIMIGDLNLVSTLLSNFFLAAYSLINFSCFHASLIKSPGWRPSFTYYNSWVSLVGGILCLIVMFLIDWKTALITFIVILTLYLFVAYRKPDVNWGSSTQAQTYVSALKSTLDLITVEEHVKNYRPQLLVLTGPPGTRPPLLDFAQSITKNISLLACGHVIQGPQTQRVRNSLTRRAYNWLTRHKIRAFYSIVEANNLEEGSRNLFQLVGLGKLRPNIVLLGYKSNWAKCDRLELRAYFNTLHEALDMYYGVAILRVPQGLDYSQI-VEDEDTPVVMNGNETTGAAVAEEIKRNQSAGQLSQDGTGSEASTPPGSPQAERAGTAAVTTEPSGVEA-------LKRRASLANLYRGPGGVELGKDVLNNITTFRRKQ-KKGTIDVWWLYDDGGLTLLLPYILTTRSQWSNCTLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKAKS---------NVEIDKEN--EGLWISEAELLGQREKTNRHVRLRELLLENSREASLVVMTLPMPRKNSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLT 1057 HSP 2 Score: 848.581 bits (2191), Expect = 0.000e+0 Identity = 465/1000 (46.50%), Postives = 644/1000 (64.40%), Query Frame = 0
Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMI-YQFSSQ--FDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPE--ELLLDREDELYFQNCSNRNCT---------YGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEK-VMTAASQASGASDEDRFSMLNHALSLDEF-------------QPKSVQLSSKNVDSFGTSSTRNNSEVS--YGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGDEQGVQRPI--------VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RPTLD+L N + QS AD +A+ T + +FGWI+GV + CLLNIWGVMLFLR++W+VGQAGLL S II +AN+VT VT +SMSAV TNG+IKGGG+Y+MISRSLGPEFGG++GLMFT A SIA + YIIGFC++L D++ Y F++ DG + ND RI+G+ TL+ +L ++ +GM V ++Q+ LL +L+ SQ+DF+IG F+ P D +R+ G VG + N DF + DG F VF VFFPAVTG+VAGAN+SGDLKDPGSAIPKGTL AI T+ +Y++Y ++A S +R ASGN E ++ + L +N +CT +G + Q+++L S WGPLIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P I SF RG+G + +P+RGY+L+F I+ CI+IGDLN VS+LLSNFF+A Y++IN+S FHAS SPGWRPSF Y+N W+SL+G LC++VM L+D TAL T I I+ LY+FV R PDVNWGSS+Q+Q++++ALKS L VE+HVKNYRP+L+V+TG P RP L DFA ITK SLL HVI+ R+ +++ ++WL + I+AFY++ ++ G R +L GLGKL N++++G++ W + Y+ L + DM+ V +LRV GLD S I + V+ ++ +GA+ + A L + Q + ++ + G + + + ++ Y G + + ++ +T FR K QK GTIDV+WLYDDGGLTLLLPYIL+TR +++ C +RVF L+NR D L E MA+LL KFRI++ DVI++ DV K + + F ++ + I +SE LL ++T H+R+ E+ ENS ++LVVMTLP+PR+N + LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct: 79 RPTLDDL----HNNTFR----EQQQSTGADPEAQATNLRGK-VIKFGWIEGVLMRCLLNIWGVMLFLRVSWMVGQAGLLYSLMIIIMANIVTVVTTLSMSAVATNGRIKGGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLQDLLKYYFNNAQIVDGAI-------NDTRIVGTITLIAVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVIGFFMGPLDNLERSQGFVGMSSGVFSHNFASDF-RYFDGVHYEFFGVFGVFFPAVTGIVAGANLSGDLKDPGSAIPKGTLLAIGITFVTYIVYPFMLAGSVVRDASGNVTMFEAYVNSTELLENPVFTNESCTTDGGGGTCEFGLQNSFQVMELVSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIYSFSRGYGANNDPVRGYILVFVISFICIMIGDLNLVSTLLSNFFLAAYSLINFSCFHASLIKSPGWRPSFTYYNSWVSLVGGILCLIVMFLIDWKTALITFIVILTLYLFVAYRKPDVNWGSSTQAQTYVSALKSTLDLITVEEHVKNYRPQLLVLTGPPGTRPPLLDFAQSITKNISLLACGHVIQGPQTQRVRNSLTRRAYNWLTRHKIRAFYSIVEANNLEEGSRNLFQLVGLGKLRPNIVLLGYKSNWAKCDRLELRAYFNTLHEALDMYYGVAILRVPQGLDYSQIVEDEDTPVVMNGNETTGAAVAEEIKRNQSAGQLSQDGTGSEASTPPGSPQAERAGTAAVTTEPSGVEALKRRASLANLYRGPGGVELGKDVLNNITTFRRK-QKKGTIDVWWLYDDGGLTLLLPYILTTRSQWSNCTLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKAKSNVEIDKENEGLWISEAELLGQREKTNRHVRLRELLLENSREASLVVMTLPMPRKNSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLTFYS 1060
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1061481000|gb|ODM98387.1| (Bumetanide-sensitive sodium-(potassium)-chloride cotransporter [Orchesella cincta]) HSP 1 Score: 989.564 bits (2557), Expect = 0.000e+0 Identity = 514/982 (52.34%), Postives = 686/982 (69.86%), Query Frame = 0
Query: 196 EGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSG-VQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEG--RKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKA--QMIF-----------ARNRTTSELSEQSLNFEDGSESKMSVDSGL-----ASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
+GK +KFGW +GV MRC+LNIWGVMLFLRL+WVIG AG+LEG +I+L+N+VT IT SMSAVSTNGQIK GGIYYMISRSLGPEFG AIG+MFT+ANSIAVSMYI+GFCESL D+L+ D I+D ND+R++G L+ IL LA VGM+WVTR QM LL++L+ +QVDFI+GS I P DEE AKGF+ Y+ A +N + Y D +G Q +FFS F VFFPAVTGIVAGANLSGDLKDPA AIPKGTLLAI TY+TY++Y +M+ C++R A+GN E + ++ N T E Y DC+ + C+YG+L S Q+ME+IS+WGPLIY GCFAATLSSAIASLVGAPRV QA+ KDKL+P IH F++G+GANNDPVRGYILVFIISL C+LI +LN ++ LLSNFF+ AY LINFS FHA++T SPGWRP+FKYYN W+SL+G +LCI VMFLM + TA+IT C+I LY+++ +RKP+VNWGSS+Q+QS+ +ALK++Q L V++HVKNYRP+++VLSG P RP L+DFA+L+ K LSL+ Q+ + S + K +AL A WL + K F+++ ++S G R+ ++I GLGKL PN VLMG+K DWQ+ + + +EY+ + A DM L V +LR+ GLD + + +++A Q I RN++ S+LS +DG+ S+ S ++PA T + +G +++ K S+S +G + + + +TQF+ K + GTID++WLYDDGGLT+LLP+ILT+R++F+ LRIF L+ DEL + RNM ALLSKFRI++SDVI++ D+TKKA + T+ +F A+I + KS D + VTE EL E+TN LR+ E++ +S E+ +VMTLP+P++ + PLYMAWL+ T+ MPPFLM+RGNQ SVLT
Sbjct: 44 KGKVVKFGWLEGVLMRCILNIWGVMLFLRLSWVIGHAGVLEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTVANSIAVSMYIVGFCESLNDLLKA--NDLT-IIDGGS--NDIRVVGIGVLIAILVLAFVGMDWVTRTQMFLLVVLIAAQVDFIVGSFIGPVDDEERAKGFIEYNAATFATNFWADYRTDYGAGDTQQSFFSAFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYITYILYAVMMGGCALREATGN--ETQYILNM-NATFMEENPDFLLYTDCKDGLQTCDYGMLNSAQVMELISSWGPLIYMGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYILVFIISLGCLLIAELNAIAPLLSNFFLMAYFLINFSCFHATVTKSPGWRPAFKYYNAWVSLVGCVLCIAVMFLMSWVTALITVACVIFLYLYVAYRKPEVNWGSSTQAQSYNSALKSIQDLNVVEEHVKNYRPQLLVLSGMPSSRPPLIDFANLIVKNLSLMLCAQVVKGSYNQKVRNALSRQAYNWLAKHKKKGFYTMIEDESFERGCRSIMQICGLGKLRPNTVLMGYKTDWQRGNRQELKEYFNVIHEALDMYLGVAILRVQEGLDYTGVIEDEDQATPQQILLDMKKDKEKGIVRNQSESQLS------QDGTSSEASSPPSSPKIERSNPAGPTGE------TAVGLKKRQRKHSLSALYAGPNGSALPRDVISHLTQFQRKQ-KKGTIDVWWLYDDGGLTLLLPYILTSRSQFSGCALRIFSLASKKDELGREQRNMAALLSKFRIDYSDVIVIPDVTKKAHENTKKEFDAVI------------APFKSNNSD-----DGTSVTEAELIACREKTNRHLRLRELLLTHSKESTFIVMTLPMPRKGTVSAPLYMAWLEMMTKGMPPFLMIRGNQTSVLT 987 HSP 2 Score: 861.677 bits (2225), Expect = 0.000e+0 Identity = 443/967 (45.81%), Postives = 633/967 (65.46%), Query Frame = 0
Query: 1191 TVKKDTGPA------RFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPP-SDEKRAMGIVGFDPDLLRSNLWPDFQKNVDG--EQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLL--------DREDELYFQNCSN--RNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHA---LSLDEFQ------------PKSVQLSSKNVDSFGTSSTRNNSEVS--YGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS-----GDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
T + GPA +FGW++GV + C+LNIWGVMLFLRL+WV+G AG+L F +I++ANLVT +TA SMSAV TNGQIKGGGIY+MISRSLGPEFG A+GLMFT A SIAVSMYI+GFCE+L D++ ++ I G S ND+R++G L+ +L ++F+GM V + Q+ LL+VL+A+QVDF++G+F+ P DE+RA G + ++ +N W D++ + Q F F VFFPAVTG+VAGAN+SGDLKDP SAIPKGTL AI TY +Y++YA+++ +R A+GN + +L + D L + +C + + C YG L + Q+++L S WGPLIY GCFAATLSSA+ASLVG PRVFQA+ KDKL+P I F +G+G + +P+RGY+L+F I+L C+LI +LN ++ LLSNFF+ Y +IN+S FHA+ SPGWRP+FKY+N W+SL+G LCI VM LM TAL T+ C+I LY++V R P+VNWGSS+Q+QS+ +ALKS+Q LN VE+HVKNYRP+L+V++G PS RP L DFANLI K SL+ V+K ++ + + ++WL + K FY + ++SF G R+ +++ GLGKL N +++G++ W + +EY+ V+ + DM+L V +LRVQ GLD + + ++ D+++ + N + LS D +S + G + +S Y +G+ + ++ +TQF+ K QK GTIDV+WLYDDGGLTLLLPYIL++R +F+ C +R+F L+++ D L E MA+LL KFRI++ DVI++ DVTK + T F +++ + G V+E L+ ++T HLR+ E+ +S S +VMTLP+PR+ + LY+AWL+ +++ MPPFL +RGNQ SVLTFYS
Sbjct: 34 TEDDEDGPALKGKVVKFGWLEGVLMRCILNIWGVMLFLRLSWVIGHAGVLEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTVANSIAVSMYIVGFCESLNDLLK--ANDLTIIDGGS----NDIRVVGIGVLIAILVLAFVGMDWVTRTQMFLLVVLIAAQVDFIVGSFIGPVDDEERAKGFIEYNAATFATNFWADYRTDYGAGDTQQSFFSAFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYITYILYAVMMGGCALREATGNETQYILNMNATFMEENPDFLLYTDCKDGLQTCDYGMLNSAQVMELISSWGPLIYMGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYILVFIISLGCLLIAELNAIAPLLSNFFLMAYFLINFSCFHATVTKSPGWRPAFKYYNAWVSLVGCVLCIAVMFLMSWVTALITVACVIFLYLYVAYRKPEVNWGSSTQAQSYNSALKSIQDLNVVEEHVKNYRPQLLVLSGMPSSRPPLIDFANLIVKNLSLMLCAQVVKGSYNQKVRNALSRQAYNWLAKHKKKGFYTMIEDESFERGCRSIMQICGLGKLRPNTVLMGYKTDWQRGNRQELKEYFNVIHEALDMYLGVAILRVQEGLDYTGVIEDEDQATPQQILLDMKKDKEKGIVRNQSESQLSQDGTSSEASSPPSSPKIERSNPAGPTGETAVGLKKRQRKHSLSALYAGPNGSALPRDVISHLTQFQRK-QKKGTIDVWWLYDDGGLTLLLPYILTSRSQFSGCALRIFSLASKKDELGREQRNMAALLSKFRIDYSDVIVIPDVTKKAHENTKKEFDAVIAPFKSNNSDDGTS---VTEAELIACREKTNRHLRLRELLLTHSKESTFIVMTLPMPRKGTVSAPLYMAWLEMMTKGMPPFLMIRGNQTSVLTFYS 990
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1325281518|ref|XP_023325442.1| (solute carrier family 12 member 2-like [Eurytemora affinis]) HSP 1 Score: 989.178 bits (2556), Expect = 0.000e+0 Identity = 531/1014 (52.37%), Postives = 705/1014 (69.53%), Query Frame = 0
Query: 153 LSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDS--VERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVG-EILNITQAYDDC--------EGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQ-ITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
+SF + + RPT++EL DE + G E +GK IKFGW +GVYMRCLLNIWGVMLFLRLTWVIGQ G+++GL ++T +N+VT++T ISMSAV+TNGQIK GGIY+MISR+LGPEFGGAIG+MFTLANSIAVSMYIIGF ESL+DM + Y+ ++GIVD+ R+NDVR+IG ++LVLIL LA VGM+WVTRVQ GLLLLLL SQVDF+IG+ IP SD + +KGF G+ + N+ Y + +FFSVFAVFFPAVTGIVAGANLSGDLKDPA AIPKGTLLAI TTY+TY++YG++VA+ SG EE T ND++ E + +DDC G +C +G L QQ M VIS G LIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I F+ G+GANNDP+RGYILVF+ISL C+LIG+LN VSSLLSNFFVAAYALINFSVFHASIT SPGWRP+FKYY+ W+SLLGT+LC+ VMF+MD+ TA++T V +I+LY FIY+RKP+ NWGSS+Q+Q F++ALK VQ+LT DHVKNYRPK++VL+GNP RP L+DFA+L+TK+LSLL ITE K + ++ + WLN + IK F+++T + S EGA+A L + GLGKL+PN+VL+GFK DW +L +Y+ L +A+D L++G+LR+ GLD SE ++E R + + ++ ED E + + T+ V+ +++T++ + DG P+++ ++ I QF+ + + G ID++WLYDDGGLT+L+P+IL+TR + LR+F L D+LD DTRNM LL+KFRI+FSDVI++ D+TK+A T+++ K ++ D L ++E++L + E+TN LR+AE++R++S E+ +VMTLP+P++ + LYMAWL+ TR +PP L++RGNQ SVLT
Sbjct: 1 MSFGRNSGKGQRPTIEELRDEEY-----SKPEQSGAGDVEGQKKGKVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQCGIVQGLLVMTTANLVTLVTAISMSAVATNGQIKGGGIYFMISRALGPEFGGAIGLMFTLANSIAVSMYIIGFAESLLDMFQDYIPGWSGIVDADFENRLNDVRIIGVVTLVLILVLAFVGMDWVTRVQKGLLLLLLCSQVDFLIGTFIPKSDLDHSKGFTGWSLETVTKNVQPHYE-------EQSFFSVFAVFFPAVTGIVAGANLSGDLKDPATAIPKGTLLAIGTTYLTYILYGVVVAFTYSPYGSGVAEEYSIWT---NDSIPLEEKMLVPRWDDCSADATLLRNGTECRFGTLNDQQTMAVISFTGYLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPKIEFFAPGFGANNDPIRGYILVFLISLGCVLIGELNMVSSLLSNFFVAAYALINFSVFHASITKSPGWRPAFKYYSPWVSLLGTLLCVCVMFMMDWVTALLTYVIVIVLYFFIYYRKPEANWGSSAQAQVFVSALKGVQSLTETQDHVKNYRPKVLVLTGNPAHRPPLVDFANLITKKLSLLICAHVITEPG--SKHLALMRESTQAWLNDHKIKGFYTVTESGSWEEGAKACLTLAGLGKLAPNLVLIGFKSDWMTNLEGLHQYFNVLHSAYDHALSIGILRVKNGLDFSEHILAEETVGDHSEERRGSGDEEKEKEKKEDEEEGDGEDKDEIGELQSKTE--------VVQAVRQRTRTISRVTYETHDGTPLDKTIIDSIQQFQGEK-KKGFIDVWWLYDDGGLTLLIPYILSTRKMYTDCSLRVFTLGNKKDDLDRDTRNMCTLLAKFRIDFSDVIVIPDVTKRAQDATKEELKQIL-----------------------DKLPEDCISEDDLLMNKEKTNRHLRLAELLREHSKESQQIVMTLPMPRKGSVCPELYMAWLEIMTRDLPPTLLIRGNQTSVLT 965 HSP 2 Score: 892.493 bits (2305), Expect = 0.000e+0 Identity = 491/987 (49.75%), Postives = 654/987 (66.26%), Query Frame = 0
Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIV-GDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEE--------------LLLDREDELYFQNCSNRN---CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGA---------SDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RPT++EL R S + QSG D + + K +FGWI+GVY+ CLLNIWGVMLFLRLTWV+GQ G++ ++T ANLVT VTAISMSAV TNGQIKGGGIYFMISR+LGPEFGGA+GLMFT A SIAVSMYIIGF E+LLDM + + GIV D E+R NDVRI+G TLVL+L ++F+GM V ++Q LLL+LL SQVDFLIGTF+P SD + G G+ + + N+ P + E+ F VFAVFFPAVTG+VAGAN+SGDLKDP +AIPKGTL AI TTY +Y++Y +V+A + SG EE +L+ R D+ RN C +GTL +QQ + + S G LIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P IE F GFG + +P+RGY+L+F I+L C+LIG+LN VSSLLSNFFVA YA+IN+SVFHAS SPGWRP+FKY++ W+SLLGT LC+ VM +MD TAL T + +I+LY F+ R P+ NWGSS+Q+Q F++ALK +Q+L +DHVKNYRPK++V+TGNP+ RP L DFANLITK+ SLL HVI E + + M+ WL D+ IK FY +T + S+ G + + L+GLGKL+ N+++IGF+ W + +Y+ VL ++D LS+G+LRV+ GLD S+ A E V + + G+ +++ + E Q K+ + + TR S V+Y DG P+D+ I+ + QF+ + +K G IDV+WLYDDGGLTLL+PYILSTRK + C +RVF L N+ D L + M +LL KFRI+F DVI++ DVTK + T + ++ + +SE +LL + ++T HLR+AE+ +E+S S +VMTLP+PR+ + LY+AWL+ ++R +PP L +RGNQ SVLTFYS
Sbjct: 12 RPTIEEL--RDEEYS------KPEQSGAGDVEGQ----KKGKVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQCGIVQGLLVMTTANLVTLVTAISMSAVATNGQIKGGGIYFMISRALGPEFGGAIGLMFTLANSIAVSMYIIGFAESLLDMFQDYIPGWSGIVDADFENRLNDVRIIGVVTLVLILVLAFVGMDWVTRVQKGLLLLLLCSQVDFLIGTFIPKSDLDHSKGFTGWSLETVTKNVQPHY------EEQSFFSVFAVFFPAVTGIVAGANLSGDLKDPATAIPKGTLLAIGTTYLTYILYGVVVAFTYSPYGSGVAEEYSIWTNDSIPLEEKMLVPRWDDCSADATLLRNGTECRFGTLNDQQTMAVISFTGYLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPKIEFFAPGFGANNDPIRGYILVFLISLGCVLIGELNMVSSLLSNFFVAAYALINFSVFHASITKSPGWRPAFKYYSPWVSLLGTLLCVCVMFMMDWVTALLTYVIVIVLYFFIYYRKPEANWGSSAQAQVFVSALKGVQSLTETQDHVKNYRPKVLVLTGNPAHRPPLVDFANLITKKLSLLICAHVITEP-GSKHLALMRESTQAWLNDHKIKGFYTVTESGSWEEGAKACLTLAGLGKLAPNLVLIGFKSDWMTNLEGLHQYFNVLHSAYDHALSIGILRVKNGLDFSEHILAEETVGDHSEERRGSGDEEKEKEKKEDEEEGDGEDKDEIGELQSKTEVVQAVR------QRTRTISRVTYETHDGTPLDKTIIDSIQQFQGEKKK-GFIDVWWLYDDGGLTLLIPYILSTRKMYTDCSLRVFTLGNKKDDLDRDTRNMCTLLAKFRIDFSDVIVIPDVTKRAQDATKEELKQIL----DKLPEDCISEDDLLMNKEKTNRHLRLAELLREHSKESQQIVMTLPMPRKGSVCPELYMAWLEIMTRDLPPTLLIRGNQTSVLTFYS 968
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1045308339|gb|ANU78813.1| (Na+/K+/2Cl- cotransporter [Portunus trituberculatus]) HSP 1 Score: 986.097 bits (2548), Expect = 0.000e+0 Identity = 515/1036 (49.71%), Postives = 713/1036 (68.82%), Query Frame = 0
Query: 132 KPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNG--AEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNI-TQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARN-----RTTSELSEQSLN---FEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
K L+HYLT+E LP HYRN S G RPTLD+L + + N ++G++ A + GK IKFGW +GVYMRCLLNIWGVMLFLR++WV+GQ+G++ + + L N+VT +TT+SMSAV+TNG+I+AGG+YYMISRSLGPEFGG+IG+MFTLANSIA + YIIGFC+SL D++ Y D IVD +ND R++G+I+L+ +LALAIVGM+WVTRVQMGLL LL+ SQ+DF++G+ I P +D + ++GF+G VL N+ Y E G NFFSVF VFF AVTGIVAGANLSGDLKDPA AIPKGTL AIVTT+ TY+IY +M+ +R A+G+V + D N T + D + C +G+ S Q+ME++SAWGPLIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+PGI FS+G GANNDPVRGYILVF+IS ICI+IGDLN VS+LLSNFF+A+Y+LINFS FHAS+ SPGWRPSFKYYN WIS LG ILC+ VMFL+D+ TA+ T + I+ LY+F+ +R P VNWGSS+Q+Q++++ALK L +++HVKNYRP+I+VL+G RP L+DFA+ +TK +SLL + + + ++L + WL + I++F+S+ +L +GAR ++ GLGKL PN V++G+K +W++ + + Y+ TL A DM + +LR+ GLD S++ DE + + N +TT + QS +DG++S+ S G SP + + + D + KK ++S++ G ++++ + IT F+ K + GTID++WLYDDGGLT+L+P+ILTTR++++ KLR+F L+ DELD + R+M LL+KFRI++SDVI++ D+ KKA + +R +F +I + + VDK + + +L++E EL E+TN +R+ E++ +NS ++ LVVMTLP+P++ + PLYMAWL+ TR MPPFL++RGNQ SVLT
Sbjct: 48 KSLRHYLTREALPKESHYRNLDSIVDG--SGPRPTLDDLHHNT----LRENQRQQGIDAEAAAANPRGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQSGIVLAILTVILGNVVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLKDLMFYYF-DGAKIVDGA--VNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQNDVQRSQGFIGLSGEVLAKNVGPDYRNFE--GRPQNFFSVFGVFFTAVTGIVAGANLSGDLKDPAEAIPKGTLAAIVTTFCTYIIYPIMIGAAVLRDATGDVNVYQMYQNHSIDENPAFTNCSTTGHVDNGTQVCPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYILVFVISFICIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWLGGILCLIVMFLIDWITALATFLIIVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNTIEEHVKNYRPQILVLTGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLIRHRIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPMELKAYFNTLHEALDMYFGLVILRVPQGLDYSQII-EDEDSPITMNGNEGAITQTTGDKPGQSTANQLTQDGTDSEASSPPG--SPQ-VERSTVATDANT--ENSKKRRTSLANLFRGPGGSELSKDVLNNITMFKRKQ-KKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLI-----------EDFKTKDEVDKEN--DGLLISEAELLGQREKTNRHIRLRELLLENSRDSSLVVMTLPMPRKTSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLT 1052 HSP 2 Score: 862.448 bits (2227), Expect = 0.000e+0 Identity = 460/1021 (45.05%), Postives = 663/1021 (64.94%), Query Frame = 0
Query: 1134 FTTRKMPPFLMVRGNQDSVLTS---RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDG--IVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASG--NPEELLLDR--EDELYFQNCSNRN--------CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWW-LYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDI----------SGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNV--DSFGTSSTRNNSEVSYGIR--DGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV----SGDEQGVQRP--IVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+ TR+ P N DS++ RPTLD+L + E + Q A+ A + K +FGW++GVY+ CLLNIWGVMLFLR++WVVGQ+G++ + + + N+VT++T +SMSAV TNG+I+ GG+Y+MISRSLGPEFGG++GLMFT A SIA + YIIGFC++L D+++ + FDG IV + ND RI+G+ TL+ +L ++ +GM V ++Q+ LL +L+ SQ+DF++G F+ P +D +R+ G +G ++L N+ PD+ +N +G F VF VFF AVTG+VAGAN+SGDLKDP AIPKGTLAAI+TT+C+Y++Y ++I A+ +R A+G N ++ + ++ F NCS C +G + Q+++L S WGPLIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P I F +G G + +P+RGY+L+F I+ CI+IGDLN VS+LLSNFF+A+Y++IN+S FHAS SPGWRPSFKY+N WIS LG LC++VM L+D TALAT + I+ LY+FV RNP+VNWGSS+Q+Q++++ALK+ LN +E+HVKNYRP+++V+TG RP L DFA ITK SLL HVI+ R+ +++ ++WL + I+AFY+L + G R +L GLGKL N +++G++ W P + Y+ L + DM+ + +LRV GLD S I +G + G S ++ + + S P S Q+ V D+ +S + + ++ R G+ + + ++ +T F+ K QK GTIDV+WLYDDGGLTLL+PYIL+TR +++ CK+RVF L+NR D L E MA+LL KFRI++ DVI++ DV K + + F ++ + DE + ++SE LL ++T H+R+ E+ ENS S+LVVMTLP+PR+ + LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct: 53 YLTREALPKESHYRNLDSIVDGSGPRPTLDDLHHNTLR------ENQRQQGIDAEAAAANPRGK---VIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQSGIVLAILTVILGNVVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLKDLMFYY---FDGAKIV---DGAVNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQNDVQRSQGFIGLSGEVLAKNVGPDY-RNFEGRPQNFFSVFGVFFTAVTGIVAGANLSGDLKDPAEAIPKGTLAAIVTTFCTYIIYPIMIGAAVLRDATGDVNVYQMYQNHSIDENPAFTNCSTTGHVDNGTQVCPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYILVFVISFICIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWLGGILCLIVMFLIDWITALATFLIIVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNTIEEHVKNYRPQILVLTGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLIRHRIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPMELKAYFNTLHEALDMYFGLVILRVPQGLDYSQIIEDEDSPITMNGNEGAITQTTGDKPGQSTANQLTQ-DGTDSEASSPPGSPQVERSTVATDANTENSKKRRTSLANLFRGPGGSELSKDVLNNITMFKRK-QKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKTKDEVDKENDGLLISEAELLGQREKTNRHIRLRELLLENSRDSSLVVMTLPMPRKTSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLTFYS 1055
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1036124418|gb|ANJ60741.1| (Na+/K+/2Cl- cotransporter [Scylla paramamosain]) HSP 1 Score: 984.941 bits (2545), Expect = 0.000e+0 Identity = 511/1044 (48.95%), Postives = 714/1044 (68.39%), Query Frame = 0
Query: 132 KPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNG--AEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDT---------VGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLA-DAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELS------EQSLN--FEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
K L+HYLT+E LP HYRN S G +RPTLD+L + + N ++G++ A + GK IKFGW +GVYMRCLLNIWGVMLFLR++WV+GQ+G++ L + L N+VT +TT+SMSAV+TNG+I+AGG+YYMISRSLGPEFGG+IG+MFTLANSIA + YIIGFC+SL D++ Y D IVD +ND R++G+I+L+ +LALAIVGM+WVTRVQMGLL LL+ SQ+DF++G+ I P +D + A+GF+G + V+ N+ Y + E G NFFSVF VFF AVTGIVAGANLSGDLKDPA AIPKGTL AI+TT+ TY+IY +M+ +R A+G+ + +L D +G++ N TQ C +G+ S Q+ME++SAWGPLIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+PGI FS+G GANNDPVRGY+LVF+IS +CI+IGDLN VS+LLSNFF+A+Y+LINFS FHAS+ SPGWRPSFKYYN WIS +G ILC+ VMFL+D+ TA+IT + + LY+F+ +R P VNWGSS+Q+Q++++ALK L +++HVKNYRP+I+VLSG RP L+DFA+ +TK +SLL + + + ++L + WL + I++F+S+ +L +GAR ++ GLGKL PN V++G+K +W++ + + Y+ TL A DM V +LR+ GLD S++ DE + + N + S + + N +DG++S+ S + P + + G KK ++SM+ G ++++ + IT F+ K + GTID++WLYDDGGLT+L+P+ILTTR++++ KLR+F L+ DELD + R+M LL+KFRI++SDVI++ D+ KKA + +R +F +I K + VDK + + ++++E E E+TN +R+ E++ +NS ++ LVVMTLP+P++ + PLYMAWL+ TR MPPFL++RGNQ SVLT
Sbjct: 47 KSLRHYLTREALPKESHYRNLDSIVDGS---ARPTLDDLHHNT----LRENQRQQGIDAEAAAANPRGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQSGIILALLTVILGNVVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLQDLMFFYF-DGAKIVDG--EVNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQNDLQRAQGFIGLSSEVMAKNVGPDYREFENRG--QNFFSVFGVFFTAVTGIVAGANLSGDLKDPAEAIPKGTLAAILTTFCTYIIYPIMIGAAVLRDATGDKDVYVMYQNLSIDENPAFTNCSLIGKVDNGTQV--------CPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYVLVFVISFVCIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWVGGILCLIVMFLIDWVTALITFLITVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNSIEEHVKNYRPQILVLSGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLTRHSIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPLELKAYFNTLHEALDMYFGVVILRVPQGLDYSQII-EDEDSPITMNGNESAITQSPEDKPGQSTANQLTQDGTDSEAS-----SPPGSPQVERSAATTDANGENSKKRRTSMANLFRGPGGTELSKDVLNNITMFKRKQ-KKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFRTK-----------DEVDKEN--DGLMISEAEFLGQREKTNRHIRLRELLLENSRDSSLVVMTLPMPRKTSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLT 1050 HSP 2 Score: 862.448 bits (2227), Expect = 0.000e+0 Identity = 456/1019 (44.75%), Postives = 665/1019 (65.26%), Query Frame = 0
Query: 1134 FTTRKMPPFLMVRGNQDSVL--TSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGI-VGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELL----LDREDELYFQNCS--------NRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWW-LYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDI----------SGAHEKVMTAASQASGASDEDRFSM--LNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIR--DGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV----SGDEQGVQRP--IVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+ TR+ P N DS++ ++RPTLD+L + E + Q A+ A + K +FGW++GVY+ CLLNIWGVMLFLR++WVVGQ+G++ + + + N+VT++T +SMSAV TNG+I+ GG+Y+MISRSLGPEFGG++GLMFT A SIA + YIIGFC++L D+++ + FDG + D E ND RI+G+ TL+ +L ++ +GM V ++Q+ LL +L+ SQ+DF++G F+ P +D +RA G +G +++ N+ PD+++ + Q F VF VFF AVTG+VAGAN+SGDLKDP AIPKGTLAAILTT+C+Y++Y ++I A+ +R A+G+ + + L ++ F NCS + C +G + Q+++L S WGPLIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P I F +G G + +P+RGY+L+F I+ CI+IGDLN VS+LLSNFF+A+Y++IN+S FHAS SPGWRPSFKY+N WIS +G LC++VM L+D TAL T + + LY+FV RNP+VNWGSS+Q+Q++++ALK+ LN +E+HVKNYRP+++V++G RP L DFA ITK SLL HVI+ R+ +++ ++WL + I+AFY+L + G R +L GLGKL N +++G++ W P + Y+ L + DM+ V +LRV GLD S I +G + + G S ++ + + S P+ V+ S+ D+ G +S + + ++ R G + + ++ +T F+ K QK GTIDV+WLYDDGGLTLL+PYIL+TR +++ CK+RVF L+NR D L E MA+LL KFRI++ DVI++ DV K + + F ++ + DE + ++SE L ++T H+R+ E+ ENS S+LVVMTLP+PR+ + LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct: 52 YLTREALPKESHYRNLDSIVDGSARPTLDDLHHNTLR------ENQRQQGIDAEAAAANPRGK---VIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQSGIILALLTVILGNVVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLQDLMFFY---FDGAKIVDGE--VNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQNDLQRAQGFIGLSSEVMAKNVGPDYREFENRGQ-NFFSVFGVFFTAVTGIVAGANLSGDLKDPAEAIPKGTLAAILTTFCTYIIYPIMIGAAVLRDATGDKDVYVMYQNLSIDENPAFTNCSLIGKVDNGTQVCPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYVLVFVISFVCIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWVGGILCLIVMFLIDWVTALITFLITVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNSIEEHVKNYRPQILVLSGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLTRHSIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPLELKAYFNTLHEALDMYFGVVILRVPQGLDYSQIIEDEDSPITMNGNESAITQSPEDKPGQSTANQLTQDGTDSEASSPPGSPQ-VERSAATTDANGENSKKRRTSMANLFRGPGGTELSKDVLNNITMFKRK-QKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFRTKDEVDKENDGLMISEAEFLGQREKTNRHIRLRELLLENSRDSSLVVMTLPMPRKTSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLTFYS 1053 The following BLAST results are available for this feature:
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 5
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
Pagesback to top
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold1253_size52701:29378..36696- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold1253_size52701-snap-gene-0.12 ID=maker-scaffold1253_size52701-snap-gene-0.12|Name=af190129_1na+ k+ 2cl- cotransporter|organism=Tigriopus kingsejongensis|type=gene|length=7319bp|location=Sequence derived from alignment at scaffold1253_size52701:29378..36696- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'af190129_1na+ k+ 2cl- cotransporter' has the following synonyms
Add to Basket
|