duplex-specific nuclease, maker-scaffold176_size284796-snap-gene-0.14 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of duplex-specific nuclease vs. L. salmonis genes
Match: EMLSAG00000000465 (supercontig:LSalAtl2s:LSalAtl2s106:710470:724606:-1 gene:EMLSAG00000000465 transcript:EMLSAT00000000465 description:"maker-LSalAtl2s106-augustus-gene-7.8") HSP 1 Score: 652.129 bits (1681), Expect = 0.000e+0 Identity = 370/998 (37.07%), Postives = 543/998 (54.41%), Query Frame = 0
Query: 243 VEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGE--AIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL-IHGSQVEFVCHGSEEIPSLIRDGQPSLL---------LTCDDRRFLTP-----EAQGTYV-----YTLKC----------SEPQVPKILRSPRSAC-----------------------------------AAQGADDREDALEHLALIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISPKDL-ALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEA--IIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDLSQGTGAWPALL 1170
+ +G+ + + IC D + Y ++WT H++ G++I+ RD+T DRP+FR +I ++P T T++N YSK +QVK +K +LG + +G+ IID S S T YFAKGHLSPDAAF+Y +QD TY++ NVAPQFQ+FNN NWK++E + R +A +K +++YTGT L KL + S+ T I + F + Y+P P Y+WKV+++P+ N AIAF+G+N+ + + IC+N+C + D D ++GY C +V LR ++ +PDL G E + P R+G LL L + FL P EA+ T V L C E ++ KI +S + C + +G D R+ L + I +GW F+EQ++LC+DEL Y TLWTKH V+G SI D +RP+F D G RLF T+ + Q Y + Q ++ I G + G +IE+ +L+ A+GHL+PDA F+YD Q+ TY+FVN APQ+QSFNNGNW+ALE AVRD AE L +++ V TGTY +L F + FL + + IPAP Y+WKVVH+ T +A+AFVG+NN S+ +P P+C+ C ++ W DW DSL GFMYCC DL + +LD T ++ +I ++P T T++N YSK +QVK ++ + G + + +G+ IID S S T+YFAKGHLSPDAAF+Y V+QD TY++ NVAPQFQ+FNN NWK++E + R +A +K +++YTGT L N LN I++ F + Y+P P Y+WKV+++P+ N AIAF+G+N+ + + ICSN+C + D D ++GY CC+V LR ++ +PDLS+ G WP L+
Sbjct: 145 INVGFNFSSSYSPLMNICHDEKVYGTIWTHHTIRGESINNRDRTIDRPTFRTNIGRSKIYYPFTTMTQMNSQYSKSTQVKTIKKLLGIYTIMVDGKRVPIIDESRSGTHYFAKGHLSPDAAFIYSAEQDGTYFYSNVAPQFQSFNNRNWKSIESTARKWASDNKQNLEVYTGTASIL---KL-PNKQSQPTEIKM--FPSLKYVPAPMYYWKVLYDPEANEAIAFIGLNNPYERKAHNHICTNICAQTVFDDVDFYKFEAGYTMCXEVSQLRMSISSIPDLSKEGKWPELMGKLGPTPPPPTRNGCKILLDKLPEKNTPLITSNGSFLYPTYIKDEARITLVPQGSTVELNCHRSRGNFLLYKEERISKI-KSVKLTCTNDKLYTEGMEVNPADYKCSSKNQPSLIITRNSKCSPEGIDKRKTDLGRITHISLGWNFRSGFIEQVELCIDELFYGTLWTKHNVVGKSIEFSDKDSDRPAFIVDETGQKRLFGKRSTN-KITQAYAKKSQKKTIKEITGHT-TIYGLPMIETNRKGTLFMAKGHLSPDAAFVYDGEQEGTYFFVNAAPQYQSFNNGNWRALELAVRDLAEKLHSTLTVYTGTYEILEFYE-----KQIFLLEK---KLIPAPRYFWKVVHDPSTKKAVAFVGYNNVFSKTSPKPICKDVCDQIPWVDWERDSLYKGFMYCCEV--------DDLNKAISYSPVLDASLLTDMET-----------------------------------NIGKSNIYYPFTTMTQMNSQYSKSTQVKTIETLFGNNSIIVDGKEIPIIDESRSGTNYFAKGHLSPDAAFIYSVEQDGTYFYSNVAPQFQSFNNRNWKSIESTARKWASDNKRNLEVYTGTASIL--NLLNEQCKP----INIELFSDRQYVPAPMYYWKVLYDPEANEAIAFIGLNNPYERKAHNHICSNICAQTVFDDVDFYKFEAGYTMCCEVSQLRMSISSIPDLSK-EGKWPELM 1075 HSP 2 Score: 384.03 bits (985), Expect = 8.329e-113 Identity = 214/572 (37.41%), Postives = 313/572 (54.72%), Query Frame = 0
Query: 1 MGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISPKDL-ALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAI--IDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL-IHGSQVEFVCHGSEEIPSLIRDGQPSLLLTCDDRRFLTPEAQGTYVY 568
+G SI D +RP+F D G RLF T+ + Q Y + Q ++ I G + G +IE+ +L+ A+GHL+PDA F+YD Q+ TY+FVN APQ+QSFNNGNW+ALE AVRD AE L +++ V TGTY +L F + FL + + IPAP Y+WKVVH+ T +A+AFVG+NN S+ +P P+C+ C ++ W DW DSL GFMYCC DL + +LD T ++ +I ++P T T++N YSK +QVK ++ + G + + +G+ I ID S S T+YFAKGHLSPDAAF+Y V+QD TY++ NVAPQFQ+FNN NWK++E + R +A +K +++YTGT L N LN I++ F + Y+P P Y+WKV+++P+ N AIAF+G+N+ + + ICSN+C + D D ++GY CC+V LR ++ +PDL G E + P R+G LL ++ + G+++Y
Sbjct: 601 VGKSIEFSDKDSDRPAFIVDETGQKRLFGKRSTN-KITQAYAKKSQKKTIKEITGHT-TIYGLPMIETNRKGTLFMAKGHLSPDAAFVYDGEQEGTYFFVNAAPQYQSFNNGNWRALELAVRDLAEKLHSTLTVYTGTYEILEFYE-----KQIFLLEK---KLIPAPRYFWKVVHDPSTKKAVAFVGYNNVFSKTSPKPICKDVCDQIPWVDWERDSLYKGFMYCCEV--------DDLNKAISYSPVLDASLLTDMET-----------------------------------NIGKSNIYYPFTTMTQMNSQYSKSTQVKTIETLFGNNSIIVDGKEIPIIDESRSGTNYFAKGHLSPDAAFIYSVEQDGTYFYSNVAPQFQSFNNRNWKSIESTARKWASDNKRNLEVYTGTASIL--NLLNEQCKP----INIELFSDRQYVPAPMYYWKVLYDPEANEAIAFIGLNNPYERKAHNHICSNICAQTVFDDVDFYKFEAGYTMCCEVSQLRMSISSIPDLSKEGKWPELMGKLGPTPPPPTRNGCKILLDKLPEKNTPLITSNGSFLY 1113 HSP 3 Score: 261.536 bits (667), Expect = 1.551e-71 Identity = 140/389 (35.99%), Postives = 208/389 (53.47%), Query Frame = 0
Query: 481 CSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDLIHGSQVEFVCHGSEEIPSLIRDGQ----PSLLLTCDDRRFLTPEAQGTYVYTLKCSEPQVPKILRSPRSACAAQGADDREDALEHLALIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC 865
C + D++P + + + ++Y +KD + VP GS VE CH S L ++ + S+ LTC + + T E KCS P ++ + S C+ +G D R+ LE + I +GW ++EQ+++C+DEL Y TLWTKH V G +I +RD RP+F D G RLF T+ Q+ + QN + I+ S+ Y A+GHL+PD+ F+YD Q+ TY+FVNVAPQ+QSFN GNW ALE AVRD A++ + + V TGTY +L + FL + ++IP P Y+WKV+H+ +A+AFVG+NN + +P P+C C ++ W DW +SL G+MYCC
Sbjct: 1090 CKILLDKLPEKNTPLITSNGSFLYPTYIKD-DARITLVP---QGSTVELNCHRSRGNFLLYKEERVSKIESVKLTCTNDKLYT-EGMEVNPADYKCSSKNQPSLIITRNSKCSPEGIDKRKTDLERITHISLGWNFRSGYIEQVEICIDELFYGTLWTKHYVEGQNIEMRDKYSGRPAFIVDETGKKRLFGKRSTN-----------QITKAYAKNSQNTSIYDQSIMNPSKSSKFYLAKGHLSPDSAFVYDGEQEGTYFFVNVAPQYQSFNKGNWLALEYAVRDLAKNQYSKLTVYTGTYEILELHQ-----KQIFLLEK---KFIPVPRYFWKVLHDPARKKAVAFVGYNNVLRKTSPKPICTDVCDQIPWVDWERESLFKGYMYCC 1454 HSP 4 Score: 260.766 bits (665), Expect = 2.645e-71 Identity = 132/293 (45.05%), Postives = 180/293 (61.43%), Query Frame = 0
Query: 230 INGRSISPKDLALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYL-FYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 521
I+ R + + +GW F QV++C D Y +LWTKH+V GK+I+F DK SDRP+F D TG R F + ++ +AY+KKSQ K +K + G T G +I+++ T + AKGHLSPDAAFVYD +Q+ TY+F+N APQ+Q+FNNGNW+ALE +VRD A + +YTGTY L FY K I L+ K +P P+YFWKVVH+P T A+AFVG N+ +P IC +VCD++P DW+ D+L G+MYCC+V DL + Y P L
Sbjct: 555 IDKRKTDLGRITHISLGWNFRSGFIEQVELCIDELFYGTLWTKHNVVGKSIEFSDKDSDRPAFIVDETGQKRLFGKRSTNKITQAYAKKSQKKTIKEITG--HTTIYGLPMIETNRKGTLFMAKGHLSPDAAFVYDGEQEGTYFFVNAAPQYQSFNNGNWRALELAVRDLAEKLHSTLTVYTGTYEILEFYEK----------QIFLLE---KKLIPAPRYFWKVVHDPSTKKAVAFVGYNNVFSKTSPKPICKDVCDQIPWVDWERDSLYKGFMYCCEVDDLNKAISYSPVL 832 HSP 5 Score: 241.891 bits (616), Expect = 2.528e-65 Identity = 123/292 (42.12%), Postives = 175/292 (59.93%), Query Frame = 0
Query: 230 INGRSISPKDLALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 521
I+ R + + + +GW + QV+IC D Y +LWTKH V G+ I+ RDK S RP+F D TG R F + ++ +AY+K SQ + ++I++ S SS Y AKGHLSPD+AFVYD +Q+ TY+F+NVAPQ+Q+FN GNW ALE +VRD A + + +YTGTY L ++ I L+ K ++PVP+YFWKV+H+P A+AFVG N+ +P IC++VCD++P DW+ ++L GYMYCC V+DL + Y PDL
Sbjct: 1201 IDKRKTDLERITHISLGWNFRSGYIEQVEICIDELFYGTLWTKHYVEGQNIEMRDKYSGRPAFIVDETGKKRLFGKRSTNQITKAYAKNSQNTSIY-----------DQSIMNPSKSSKFYLAKGHLSPDSAFVYDGEQEGTYFFVNVAPQYQSFNKGNWLALEYAVRDLAKNQYSKLTVYTGTYEILELHQKQ---------IFLLE---KKFIPVPRYFWKVLHDPARKKAVAFVGYNNVLRKTSPKPICTDVCDQIPWVDWERESLFKGYMYCCNVEDLNKAISYSPDL 1469 HSP 6 Score: 241.891 bits (616), Expect = 2.528e-65 Identity = 123/292 (42.12%), Postives = 175/292 (59.93%), Query Frame = 0
Query: 868 INGRSISPKDLALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 1159
I+ R + + + +GW + QV+IC D Y +LWTKH V G+ I+ RDK S RP+F D TG R F + ++ +AY+K SQ + ++I++ S SS Y AKGHLSPD+AFVYD +Q+ TY+F+NVAPQ+Q+FN GNW ALE +VRD A + + +YTGTY L ++ I L+ K ++PVP+YFWKV+H+P A+AFVG N+ +P IC++VCD++P DW+ ++L GYMYCC V+DL + Y PDL
Sbjct: 1201 IDKRKTDLERITHISLGWNFRSGYIEQVEICIDELFYGTLWTKHYVEGQNIEMRDKYSGRPAFIVDETGKKRLFGKRSTNQITKAYAKNSQNTSIY-----------DQSIMNPSKSSKFYLAKGHLSPDSAFVYDGEQEGTYFFVNVAPQYQSFNKGNWLALEYAVRDLAKNQYSKLTVYTGTYEILELHQKQ---------IFLLE---KKFIPVPRYFWKVLHDPARKKAVAFVGYNNVLRKTSPKPICTDVCDQIPWVDWERESLFKGYMYCCNVEDLNKAISYSPDL 1469 HSP 7 Score: 210.69 bits (535), Expect = 1.584e-55 Identity = 124/347 (35.73%), Postives = 196/347 (56.48%), Query Frame = 0
Query: 524 GSQVEFVCHGSEEIPSL---IRDGQPSLLLTCDDRRFLTPEAQGTYVYTLKCSEPQVPKILRSPRSACAAQGADDREDALEHLALIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILG-QNEDVNGNQ--IIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYC 864
G + CHGS+ + I G + L+CD+ F + V L C+ P++ R C+ GADDR L+ L LI +G+ + + + +C DE +Y T+WT H + G SIN RD T +RP+F+ +I G ++++ F T T + Y +S Q+ ++ +LG V+G + II+ S + YFA+GHL+PDA FIY QD TY++ NVAPQFQSFNN NWK++E R +A K +++V TGT +L+ N + +P + +++PAP YYWKV+++ + NEAIAF+G NNP+ ++ +C + C + + D + +G+ C
Sbjct: 62 GENIVIDCHGSKLSKATHYGIPSGLSKISLSCDNGAFRN-SPKIVKVEILSCTSKVYPQLERK-SVKCSPVGADDRLTDLDDLVLINVGFNFSSSYSPLMNICHDEKVYGTIWTHHTIRGESINNRDRTIDRPTFRTNI-GRSKIYYPFTTMTQMNSQYSKSTQVKTIKKLLGIYTIMVDGKRVPIIDESRSGTHYFAKGHLSPDAAFIYSAEQDGTYFYSNVAPQFQSFNNRNWKSIESTARKWASDNKQNLEVYTGTASILKLPNK--QSQPTEIKMFPSLKYVPAPMYYWKVLYDPEANEAIAFIGLNNPYERKAHNHICTNICAQTVFDDVDFYKFEAGYTMC 403 HSP 8 Score: 179.104 bits (453), Expect = 1.098e-45 Identity = 90/226 (39.82%), Postives = 128/226 (56.64%), Query Frame = 0
Query: 2 GASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC 227
G +I +RD RP+F D G RLF T+ Q+ + QN + I+ S+ Y A+GHL+PD+ F+YD Q+ TY+FVNVAPQ+QSFN GNW ALE AVRD A++ + + V TGTY +L + FL + ++IP P Y+WKV+H+ +A+AFVG+NN + +P P+C C ++ W DW +SL G+MYCC
Sbjct: 1248 GQNIEMRDKYSGRPAFIVDETGKKRLFGKRSTN-----------QITKAYAKNSQNTSIYDQSIMNPSKSSKFYLAKGHLSPDSAFVYDGEQEGTYFFVNVAPQYQSFNKGNWLALEYAVRDLAKNQYSKLTVYTGTYEILELHQ-----KQIFLLEK---KFIPVPRYFWKVLHDPARKKAVAFVGYNNVLRKTSPKPICTDVCDQIPWVDWERESLFKGYMYCC 1454 HSP 9 Score: 170.244 bits (430), Expect = 6.750e-43 Identity = 89/228 (39.04%), Postives = 140/228 (61.40%), Query Frame = 0
Query: 2 GASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILG-QNEDVNGNQ--IIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYC 226
G SIN RD T +RP+F+ +I G ++++ F T T + Y +S Q+ ++ +LG V+G + II+ S + YFA+GHL+PDA FIY QD TY++ NVAPQFQSFNN NWK++E R +A K +++V TGT +L+ N + +P + +++PAP YYWKV+++ + NEAIAF+G NNP+ ++ +C + C + + D + +G+ C
Sbjct: 179 GESINNRDRTIDRPTFRTNI-GRSKIYYPFTTMTQMNSQYSKSTQVKTIKKLLGIYTIMVDGKRVPIIDESRSGTHYFAKGHLSPDAAFIYSAEQDGTYFYSNVAPQFQSFNNRNWKSIESTARKWASDNKQNLEVYTGTASILKLPNK--QSQPTEIKMFPSLKYVPAPMYYWKVLYDPEANEAIAFIGLNNPYERKAHNHICTNICAQTVFDDVDFYKFEAGYTMC 403
BLAST of duplex-specific nuclease vs. L. salmonis genes
Match: EMLSAG00000012345 (supercontig:LSalAtl2s:LSalAtl2s900:155793:157148:-1 gene:EMLSAG00000012345 transcript:EMLSAT00000012345 description:"maker-LSalAtl2s900-snap-gene-0.28") HSP 1 Score: 152.525 bits (384), Expect = 2.495e-40 Identity = 94/269 (34.94%), Postives = 134/269 (49.81%), Query Frame = 0
Query: 604 ALIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGA--SINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADF--IYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADW---NMDSLSSGFMYCC 865
+I IGW + F Q+ +C + T ++ H + G+ S + RPS+ ++ + F T +S +Y Q + I G D S Y ARGHL PD DF I++ DATY+++NVAP +QS NNGNWK LEG++R Y L V TGTYG+L+ N G P +L ED + IP P+ WK++ N + EAI F+G NNP +Q T +C C W D+ +S G++YCC
Sbjct: 102 VMIDIGWTSDTNFTTQLSVCHNIADETTWFSNHTIQGSLSSFGTFEKDDSRPSYFKE-------GSQFFTKSSASTSYKLRTQKDKFIKIFGAQNDF-------FSIRRSFYLARGHLTPDGDFDLIHNSLADATYHYINVAPMWQSINNGNWKVLEGSIRLYVSLLSEDFVVYTGTYGVLKLPNKNGVKIPIYL-GED--ELIPVPKLIWKILFNPRREEAIVFIGSNNPFLEQIET-VCPDLCEDTHWNDFLEHRRFEVSKGYLYCC 352 HSP 2 Score: 150.214 bits (378), Expect = 1.596e-39 Identity = 89/289 (30.80%), Postives = 151/289 (52.25%), Query Frame = 0
Query: 241 ALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFR--DKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAF--VYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKL-NSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMD---NLDSGYMYCCKVKDLRDTVDYVPDL 521
+++IGW D F TQ+ +C + + ++ H++ G F +K RPS+ ++ ++FF T++ + +Y ++Q + + G S + Y A+GHL+PD F +++ DATY+++NVAP +Q+ NNGNWK LEGS+R Y +YTGTYG L KL N +G Y+ G + +PVPK WK++ NP+ AI F+G N+ L+ + +C ++C++ D+ + GY+YCC ++ + ++PDL
Sbjct: 102 VMIDIGWTSDTNFTTQLSVCHNIADETTWFSNHTIQGSLSSFGTFEKDDSRPSYFKE---GSQFF---TKSSASTSYKLRTQKDKFIKIFGAQNDF--------FSIRRSFYLARGHLTPDGDFDLIHNSLADATYHYINVAPMWQSINNGNWKVLEGSIRLYVSLLSEDFVVYTGTYGVL---KLPNKNGVKIPIYL-----GEDELIPVPKLIWKILFNPRREEAIVFIGSNNPFLEQIET-VCPDLCEDTHWNDFLEHRRFEVSKGYLYCCSYHSVKKVISWLPDL 367 HSP 3 Score: 150.214 bits (378), Expect = 1.596e-39 Identity = 89/289 (30.80%), Postives = 151/289 (52.25%), Query Frame = 0
Query: 879 ALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFR--DKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAF--VYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKL-NSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMD---NLDSGYMYCCKVKDLRDTVDYVPDL 1159
+++IGW D F TQ+ +C + + ++ H++ G F +K RPS+ ++ ++FF T++ + +Y ++Q + + G S + Y A+GHL+PD F +++ DATY+++NVAP +Q+ NNGNWK LEGS+R Y +YTGTYG L KL N +G Y+ G + +PVPK WK++ NP+ AI F+G N+ L+ + +C ++C++ D+ + GY+YCC ++ + ++PDL
Sbjct: 102 VMIDIGWTSDTNFTTQLSVCHNIADETTWFSNHTIQGSLSSFGTFEKDDSRPSYFKE---GSQFF---TKSSASTSYKLRTQKDKFIKIFGAQNDF--------FSIRRSFYLARGHLTPDGDFDLIHNSLADATYHYINVAPMWQSINNGNWKVLEGSIRLYVSLLSEDFVVYTGTYGVL---KLPNKNGVKIPIYL-----GEDELIPVPKLIWKILFNPRREEAIVFIGSNNPFLEQIET-VCPDLCEDTHWNDFLEHRRFEVSKGYLYCCSYHSVKKVISWLPDL 367 HSP 4 Score: 140.969 bits (354), Expect = 2.441e-36 Identity = 83/219 (37.90%), Postives = 115/219 (52.51%), Query Frame = 0
Query: 16 SFQRD--IPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADF--IYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADW---NMDSLSSGFMYCC 227
+F++D P + + + F T +S +Y Q + I G D S Y ARGHL PD DF I++ DATY+++NVAP +QS NNGNWK LEG++R Y L V TGTYG+L+ N G P +L ED + IP P+ WK++ N + EAI F+G NNP +Q T +C C W D+ +S G++YCC
Sbjct: 145 TFEKDDSRPSYFKEGSQFFTKSSASTSYKLRTQKDKFIKIFGAQNDF-------FSIRRSFYLARGHLTPDGDFDLIHNSLADATYHYINVAPMWQSINNGNWKVLEGSIRLYVSLLSEDFVVYTGTYGVLKLPNKNGVKIPIYL-GED--ELIPVPKLIWKILFNPRREEAIVFIGSNNPFLEQIET-VCPDLCEDTHWNDFLEHRRFEVSKGYLYCC 352
BLAST of duplex-specific nuclease vs. nr
Match: gi|1325283601|ref|XP_023326504.1| (uncharacterized protein LOC111699940 isoform X2 [Eurytemora affinis]) HSP 1 Score: 299.286 bits (765), Expect = 2.266e-87 Identity = 155/362 (42.82%), Postives = 215/362 (59.39%), Query Frame = 0
Query: 520 DLIHGSQVEFVCHGS---EEIPSLIRDGQP---SLLLTCDDRRFLTPEAQGTYVYTLKCSEPQVPKILRSPRSACAAQGADDREDALEHLALIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISP 875
D+ G ++E CHG E+ + G P S++LTC F E Q V + C+ Q P ++R+ C+ +GAD R L L +QIGW+I F+EQIQLC DE Y T+WT H + G SI RD+ RPSF+ D + + F + TST L + Y + Q + +LG N +N +IE+G + S YFA+GHL+PDA FIY++ QDATYYF+N APQFQ+FNNGNWKALE RD A + + TGT+G+L + + + P +LY ++IPAP YYWKV+H+ T A AF+G NNPH+ + P LC + CG++SW DW++ L SG+MYCC + R P
Sbjct: 50 DIDEGGELELYCHGDIRYEDSTYVSVSGLPKQKSIVLTCSQGVFYYAEDQ-VKVESSTCNRRQEPHLIRT-NEICSPEGADGRTSNLVDLVRVQIGWKIGTNFIEQIQLCHDEANYGTIWTNHTIYGKSIEHRDIDEGRPSFRAD-NSYQKRFYTWYTSTKLNEQYSKRTQGYTVSKLLGSN-IINSVPLIETGTTGSNYFAKGHLSPDAAFIYNVQQDATYYFMNAAPQFQAFNNGNWKALEYNARDLASRSGRDLSIHTGTFGVLSYTDVNNEDVPIYLYISGETRYIPAPLYYWKVIHDPSTGSAAAFIGLNNPHATEQPEELCNNVCGEMSWVDWSITELDSGYMYCCSVEDARKAIP 407 HSP 2 Score: 291.582 bits (745), Expect = 1.509e-84 Identity = 137/291 (47.08%), Postives = 190/291 (65.29%), Query Frame = 0
Query: 231 NGRSISPKDLALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 521
+GR+ + DL V+IGW + F Q+++C D Y ++WT H++YGK+I+ RD RPSFR D + RF+ T T+LNE YSK++Q + +LG + N +I++ ++ ++YFAKGHLSPDAAF+Y+VQQDATYYFMN APQFQAFNNGNWKALE + RD A S + I+TGT+G L Y +N++ YI G Y+P P Y+WKV+H+P T A AF+G+N+ H P +C+NVC E+ DW + LDSGYMYCC V+D R + +PD+
Sbjct: 128 DGRTSNLVDLVRVQIGWKIGTNFIEQIQLCHDEANYGTIWTNHTIYGKSIEHRDIDEGRPSFRADNSYQKRFYTWYTSTKLNEQYSKRTQGYTVSKLLGSNII--NSVPLIETGTTGSNYFAKGHLSPDAAFIYNVQQDATYYFMNAAPQFQAFNNGNWKALEYNARDLASRSGRDLSIHTGTFGVLSYTDVNNEDVPIYLYIS----GETRYIPAPLYYWKVIHDPSTGSAAAFIGLNNPHATEQPEELCNNVCGEMSWVDWSITELDSGYMYCCSVEDARKAIPSIPDI 412 HSP 3 Score: 291.582 bits (745), Expect = 1.509e-84 Identity = 137/291 (47.08%), Postives = 190/291 (65.29%), Query Frame = 0
Query: 869 NGRSISPKDLALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 1159
+GR+ + DL V+IGW + F Q+++C D Y ++WT H++YGK+I+ RD RPSFR D + RF+ T T+LNE YSK++Q + +LG + N +I++ ++ ++YFAKGHLSPDAAF+Y+VQQDATYYFMN APQFQAFNNGNWKALE + RD A S + I+TGT+G L Y +N++ YI G Y+P P Y+WKV+H+P T A AF+G+N+ H P +C+NVC E+ DW + LDSGYMYCC V+D R + +PD+
Sbjct: 128 DGRTSNLVDLVRVQIGWKIGTNFIEQIQLCHDEANYGTIWTNHTIYGKSIEHRDIDEGRPSFRADNSYQKRFYTWYTSTKLNEQYSKRTQGYTVSKLLGSNII--NSVPLIETGTTGSNYFAKGHLSPDAAFIYNVQQDATYYFMNAAPQFQAFNNGNWKALEYNARDLASRSGRDLSIHTGTFGVLSYTDVNNEDVPIYLYIS----GETRYIPAPLYYWKVIHDPSTGSAAAFIGLNNPHATEQPEELCNNVCGEMSWVDWSITELDSGYMYCCSVEDARKAIPSIPDI 412 HSP 4 Score: 222.246 bits (565), Expect = 8.420e-60 Identity = 108/236 (45.76%), Postives = 149/236 (63.14%), Query Frame = 0
Query: 2 GASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISP 237
G SI RD+ RPSF+ D + + F + TST L + Y + Q + +LG N +N +IE+G + S YFA+GHL+PDA FIY++ QDATYYF+N APQFQ+FNNGNWKALE RD A + + TGT+G+L + + + P +LY ++IPAP YYWKV+H+ T A AF+G NNPH+ + P LC + CG++SW DW++ L SG+MYCC + R P
Sbjct: 174 GKSIEHRDIDEGRPSFRAD-NSYQKRFYTWYTSTKLNEQYSKRTQGYTVSKLLGSN-IINSVPLIETGTTGSNYFAKGHLSPDAAFIYNVQQDATYYFMNAAPQFQAFNNGNWKALEYNARDLASRSGRDLSIHTGTFGVLSYTDVNNEDVPIYLYISGETRYIPAPLYYWKVIHDPSTGSAAAFIGLNNPHATEQPEELCNNVCGEMSWVDWSITELDSGYMYCCSVEDARKAIP 407
BLAST of duplex-specific nuclease vs. nr
Match: gi|1325283599|ref|XP_023326503.1| (uncharacterized protein LOC111699940 isoform X1 [Eurytemora affinis]) HSP 1 Score: 298.901 bits (764), Expect = 4.408e-87 Identity = 155/362 (42.82%), Postives = 215/362 (59.39%), Query Frame = 0
Query: 520 DLIHGSQVEFVCHGS---EEIPSLIRDGQP---SLLLTCDDRRFLTPEAQGTYVYTLKCSEPQVPKILRSPRSACAAQGADDREDALEHLALIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISP 875
D+ G ++E CHG E+ + G P S++LTC F E Q V + C+ Q P ++R+ C+ +GAD R L L +QIGW+I F+EQIQLC DE Y T+WT H + G SI RD+ RPSF+ D + + F + TST L + Y + Q + +LG N +N +IE+G + S YFA+GHL+PDA FIY++ QDATYYF+N APQFQ+FNNGNWKALE RD A + + TGT+G+L + + + P +LY ++IPAP YYWKV+H+ T A AF+G NNPH+ + P LC + CG++SW DW++ L SG+MYCC + R P
Sbjct: 65 DIDEGGELELYCHGDIRYEDSTYVSVSGLPKQKSIVLTCSQGVFYYAEDQ-VKVESSTCNRRQEPHLIRT-NEICSPEGADGRTSNLVDLVRVQIGWKIGTNFIEQIQLCHDEANYGTIWTNHTIYGKSIEHRDIDEGRPSFRAD-NSYQKRFYTWYTSTKLNEQYSKRTQGYTVSKLLGSN-IINSVPLIETGTTGSNYFAKGHLSPDAAFIYNVQQDATYYFMNAAPQFQAFNNGNWKALEYNARDLASRSGRDLSIHTGTFGVLSYTDVNNEDVPIYLYISGETRYIPAPLYYWKVIHDPSTGSAAAFIGLNNPHATEQPEELCNNVCGEMSWVDWSITELDSGYMYCCSVEDARKAIP 422 HSP 2 Score: 291.582 bits (745), Expect = 2.201e-84 Identity = 137/291 (47.08%), Postives = 190/291 (65.29%), Query Frame = 0
Query: 231 NGRSISPKDLALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 521
+GR+ + DL V+IGW + F Q+++C D Y ++WT H++YGK+I+ RD RPSFR D + RF+ T T+LNE YSK++Q + +LG + N +I++ ++ ++YFAKGHLSPDAAF+Y+VQQDATYYFMN APQFQAFNNGNWKALE + RD A S + I+TGT+G L Y +N++ YI G Y+P P Y+WKV+H+P T A AF+G+N+ H P +C+NVC E+ DW + LDSGYMYCC V+D R + +PD+
Sbjct: 143 DGRTSNLVDLVRVQIGWKIGTNFIEQIQLCHDEANYGTIWTNHTIYGKSIEHRDIDEGRPSFRADNSYQKRFYTWYTSTKLNEQYSKRTQGYTVSKLLGSNII--NSVPLIETGTTGSNYFAKGHLSPDAAFIYNVQQDATYYFMNAAPQFQAFNNGNWKALEYNARDLASRSGRDLSIHTGTFGVLSYTDVNNEDVPIYLYIS----GETRYIPAPLYYWKVIHDPSTGSAAAFIGLNNPHATEQPEELCNNVCGEMSWVDWSITELDSGYMYCCSVEDARKAIPSIPDI 427 HSP 3 Score: 291.582 bits (745), Expect = 2.201e-84 Identity = 137/291 (47.08%), Postives = 190/291 (65.29%), Query Frame = 0
Query: 869 NGRSISPKDLALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 1159
+GR+ + DL V+IGW + F Q+++C D Y ++WT H++YGK+I+ RD RPSFR D + RF+ T T+LNE YSK++Q + +LG + N +I++ ++ ++YFAKGHLSPDAAF+Y+VQQDATYYFMN APQFQAFNNGNWKALE + RD A S + I+TGT+G L Y +N++ YI G Y+P P Y+WKV+H+P T A AF+G+N+ H P +C+NVC E+ DW + LDSGYMYCC V+D R + +PD+
Sbjct: 143 DGRTSNLVDLVRVQIGWKIGTNFIEQIQLCHDEANYGTIWTNHTIYGKSIEHRDIDEGRPSFRADNSYQKRFYTWYTSTKLNEQYSKRTQGYTVSKLLGSNII--NSVPLIETGTTGSNYFAKGHLSPDAAFIYNVQQDATYYFMNAAPQFQAFNNGNWKALEYNARDLASRSGRDLSIHTGTFGVLSYTDVNNEDVPIYLYIS----GETRYIPAPLYYWKVIHDPSTGSAAAFIGLNNPHATEQPEELCNNVCGEMSWVDWSITELDSGYMYCCSVEDARKAIPSIPDI 427 HSP 4 Score: 222.246 bits (565), Expect = 1.109e-59 Identity = 108/236 (45.76%), Postives = 149/236 (63.14%), Query Frame = 0
Query: 2 GASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISP 237
G SI RD+ RPSF+ D + + F + TST L + Y + Q + +LG N +N +IE+G + S YFA+GHL+PDA FIY++ QDATYYF+N APQFQ+FNNGNWKALE RD A + + TGT+G+L + + + P +LY ++IPAP YYWKV+H+ T A AF+G NNPH+ + P LC + CG++SW DW++ L SG+MYCC + R P
Sbjct: 189 GKSIEHRDIDEGRPSFRAD-NSYQKRFYTWYTSTKLNEQYSKRTQGYTVSKLLGSN-IINSVPLIETGTTGSNYFAKGHLSPDAAFIYNVQQDATYYFMNAAPQFQAFNNGNWKALEYNARDLASRSGRDLSIHTGTFGVLSYTDVNNEDVPIYLYISGETRYIPAPLYYWKVIHDPSTGSAAAFIGLNNPHATEQPEELCNNVCGEMSWVDWSITELDSGYMYCCSVEDARKAIP 422
BLAST of duplex-specific nuclease vs. nr
Match: gi|906458355|gb|KNC21579.1| (hypothetical protein FF38_05884 [Lucilia cuprina]) HSP 1 Score: 243.432 bits (620), Expect = 2.725e-63 Identity = 186/671 (27.72%), Postives = 301/671 (44.86%), Query Frame = 0
Query: 242 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSS----SSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKD-LRDTVDYVPDLIHGSQV--------EFVCHG--SEEIPSLIRDG-------QPSLLLTCD---------DRRFLTPEAQGTYV--YTLKCSEPQVP-KILRSPRSACAA----QGADDREDALEHLALIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ----QTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC 865
L+E+G+ + G F + IC D V+ + H V + ++ RP F F+ ++ Y+K Q K L +LG +D+S+ S + A+GH+ F++ Q AT+ F+NVAPQ+QAFN GNW+ +E VR Y + +D YTGT+G +N G+ K Y+ +PVPK +++VV + T I +G+N+ H + IC ++ D++ WD ++L GY Y C V D LR D + + + V F G + P + G QP+ L+ F+ P T V Y LK S V K +C+A L+++G++++ FL+ + +C DE+ T + HV L P ++QR + + +F ++ Y + QQ + +ILG + N S +LY ARGH+A DFI+ Q +T++FVNVAPQ+Q+FN GNW+ +E VR Y + +V TGT+G+ + G + +L + E+ N IP P+ Y++VV + T + I +G NNPH+ Q +C ++ W +W+ + G+ Y C
Sbjct: 216 LLEVGFEVSGGFIQTMDICHDEINEVTRYVHH-VLNPSSSVYQRSVARPRFVEG-----DFY---AGKNVDNLYTKVQQNKTLSCILG-----------MDASAYFNDSIDIFMARGHMGAKTDFIFGSPQRATFLFVNVAPQWQAFNGGNWERVEDGVRKYVSNKALTVDCYTGTWGISTLPDVN--GTQKELYLSFDE-NNNGLIPVPKIYYRVVIDRVTRQGIVLIGVNNPHATLQQVKEEYIICEDIGDKIDWLQWDKNDLHKGYSYACSVPDFLRVVKDLPVESLETTGVLGINEVGCSFRVSGDLKDPAPLFAKAGVYDWLVPQPNGLVELSYGEAIDMYCSNSFVKPFENHTKVTAYCLKGSNYLVTNKYYNFSNFSCSAWPTYTARRTGRQCNGGTDLLEVGFKVSQGFLQTMDICHDEINEVTRYVHHV----------LNPSSSAYQRSVTRPRFVEGDFYAGKNVDNLYTKVQQNKTISSILGMDASPYFND------SINLYMARGHMAAKIDFIFASPQRSTFFFVNVAPQWQAFNAGNWERVEDGVRKYVSNNALTVDCYTGTWGVSTLPDVNGTHQELYLAFDENNNGLIPVPKIYFRVVIDRATLQGIVLIGVNNPHASLQQIQKEYIVCNDISHQIDWINWSKKDIIKGYSYAC 847 HSP 2 Score: 151.369 bits (381), Expect = 1.657e-33 Identity = 93/318 (29.25%), Postives = 155/318 (48.74%), Query Frame = 0
Query: 605 LIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHS--QQTPTP--LCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISPKDL---ALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVY 914
L+++G+E++G F++ + +C DE+ T + HV L P +QR + + +F ++ Y + QQ + ILG + N I+ ++ ARGH+ DFI+ Q AT+ FVNVAPQ+Q+FN GNW+ +E VR Y + +V TGT+G+ + G + +L + E+ N IP P+ Y++VV + T + I +G NNPH+ QQ +C+ K+ W W+ + L G+ Y C + + KDL +L G + + ++ D + L+ K VY
Sbjct: 216 LLEVGFEVSGGFIQTMDICHDEINEVTRYVHHV----------LNPSSSVYQRSVARPRFVEGDFYAGKNVDNLYTKVQQNKTLSCILGMDASAYFNDSID------IFMARGHMGAKTDFIFGSPQRATFLFVNVAPQWQAFNGGNWERVEDGVRKYVSNKALTVDCYTGTWGISTLPDVNGTQKELYLSFDENNNGLIPVPKIYYRVVIDRVTRQGIVLIGVNNPHATLQQVKEEYIICEDIGDKIDWLQWDKNDLHKGYSYACSVPDFLRVV-KDLPVESLETTGVLGINEVGCSFRVSGDLKDPAPLFAKAGVY 516 HSP 3 Score: 131.724 bits (330), Expect = 2.762e-27 Identity = 82/275 (29.82%), Postives = 132/275 (48.00%), Query Frame = 0
Query: 10 LTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHS--QQTPTP--LCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISPKDL---ALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVY 276
L P +QR + + +F ++ Y + QQ + ILG + N I+ ++ ARGH+ DFI+ Q AT+ FVNVAPQ+Q+FN GNW+ +E VR Y + +V TGT+G+ + G + +L + E+ N IP P+ Y++VV + T + I +G NNPH+ QQ +C+ K+ W W+ + L G+ Y C + + KDL +L G + + ++ D + L+ K VY
Sbjct: 249 LNPSSSVYQRSVARPRFVEGDFYAGKNVDNLYTKVQQNKTLSCILGMDASAYFNDSID------IFMARGHMGAKTDFIFGSPQRATFLFVNVAPQWQAFNGGNWERVEDGVRKYVSNKALTVDCYTGTWGISTLPDVNGTQKELYLSFDENNNGLIPVPKIYYRVVIDRVTRQGIVLIGVNNPHATLQQVKEEYIICEDIGDKIDWLQWDKNDLHKGYSYACSVPDFLRVV-KDLPVESLETTGVLGINEVGCSFRVSGDLKDPAPLFAKAGVY 516 HSP 4 Score: 129.413 bits (324), Expect = 1.421e-26 Identity = 84/286 (29.37%), Postives = 134/286 (46.85%), Query Frame = 0
Query: 880 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSS----SSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVP 1157
L+E+G+ + G F + IC D V+ + H V + ++ RP F F+ ++ Y+K Q K L +LG +D+S+ S + A+GH+ F++ Q AT+ F+NVAPQ+QAFN GNW+ +E VR Y + +D YTGT+G +N G+ K Y+ +PVPK +++VV + T I +G+N+ H + IC ++ D++ WD ++L GY Y C V D V +P
Sbjct: 216 LLEVGFEVSGGFIQTMDICHDEINEVTRYVHH-VLNPSSSVYQRSVARPRFVEG-----DFY---AGKNVDNLYTKVQQNKTLSCILG-----------MDASAYFNDSIDIFMARGHMGAKTDFIFGSPQRATFLFVNVAPQWQAFNGGNWERVEDGVRKYVSNKALTVDCYTGTWGISTLPDVN--GTQKELYLSFDE-NNNGLIPVPKIYYRVVIDRVTRQGIVLIGVNNPHATLQQVKEEYIICEDIGDKIDWLQWDKNDLHKGYSYACSVPDFLRVVKDLP 478 HSP 5 Score: 129.028 bits (323), Expect = 1.889e-26 Identity = 71/223 (31.84%), Postives = 115/223 (51.57%), Query Frame = 0
Query: 10 LTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ----QTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC 227
L P ++QR + + +F ++ Y + QQ + +ILG + N S +LY ARGH+A DFI+ Q +T++FVNVAPQ+Q+FN GNW+ +E VR Y + +V TGT+G+ + G + +L + E+ N IP P+ Y++VV + T + I +G NNPH+ Q +C ++ W +W+ + G+ Y C
Sbjct: 631 LNPSSSAYQRSVTRPRFVEGDFYAGKNVDNLYTKVQQNKTISSILGMDASPYFND------SINLYMARGHMAAKIDFIFASPQRSTFFFVNVAPQWQAFNAGNWERVEDGVRKYVSNNALTVDCYTGTWGVSTLPDVNGTHQELYLAFDENNNGLIPVPKIYFRVVIDRATLQGIVLIGVNNPHASLQQIQKEYIVCNDISHQIDWINWSKKDIIKGYSYAC 847 HSP 6 Score: 120.168 bits (300), Expect = 8.217e-24 Identity = 81/295 (27.46%), Postives = 142/295 (48.14%), Query Frame = 0
Query: 880 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSS----SSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVP-DLSQGTGA 1165
L+E+G+ + F + IC D V+ + H + + ++ ++ RP F F+ ++ Y+K Q K + +LG +D+S S Y A+GH++ F++ Q +T++F+NVAPQ+QAFN GNW+ +E VR Y ++ +D YTGT+G +N G+ + Y+ +PVPK +++VV + T I +G+N+ H + +C+++ ++ +W ++ GY Y C V D +TV +P D Q TG
Sbjct: 598 LLEVGFKVSQGFLQTMDICHDEINEVTRYVHHVLNPSSSAYQ-RSVTRPRFVEG-----DFY---AGKNVDNLYTKVQQNKTISSILG-----------MDASPYFNDSINLYMARGHMAAKIDFIFASPQRSTFFFVNVAPQWQAFNAGNWERVEDGVRKYVSNNALTVDCYTGTWGVSTLPDVN--GTHQELYLAFDE-NNNGLIPVPKIYFRVVIDRATLQGIVLIGVNNPHASLQQIQKEYIVCNDISHQIDWINWSKKDIIKGYSYACTVPDFLETVKDLPLDQLQTTGV 869 HSP 7 Score: 119.013 bits (297), Expect = 2.133e-23 Identity = 77/286 (26.92%), Postives = 138/286 (48.25%), Query Frame = 0
Query: 242 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSS----SSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVP 519
L+E+G+ + F + IC D V+ + H + + ++ ++ RP F F+ ++ Y+K Q K + +LG +D+S S Y A+GH++ F++ Q +T++F+NVAPQ+QAFN GNW+ +E VR Y ++ +D YTGT+G +N G+ + Y+ +PVPK +++VV + T I +G+N+ H + +C+++ ++ +W ++ GY Y C V D +TV +P
Sbjct: 598 LLEVGFKVSQGFLQTMDICHDEINEVTRYVHHVLNPSSSAYQ-RSVTRPRFVEG-----DFY---AGKNVDNLYTKVQQNKTISSILG-----------MDASPYFNDSINLYMARGHMAAKIDFIFASPQRSTFFFVNVAPQWQAFNAGNWERVEDGVRKYVSNNALTVDCYTGTWGVSTLPDVN--GTHQELYLAFDE-NNNGLIPVPKIYFRVVIDRATLQGIVLIGVNNPHASLQQIQKEYIVCNDISHQIDWINWSKKDIIKGYSYACTVPDFLETVKDLP 860
BLAST of duplex-specific nuclease vs. nr
Match: gi|1135102754|ref|XP_019891145.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101888582 [Musca domestica]) HSP 1 Score: 245.743 bits (626), Expect = 1.030e-62 Identity = 184/682 (26.98%), Postives = 305/682 (44.72%), Query Frame = 0
Query: 242 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSS----SSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVP--DL-------------IHGSQVEFVCHGSEEIPSLI------------RDGQPSLLLTC--------DDRRFLTPEAQGTYVYTL----------KCSEPQVPKILRSPRSACAAQGADDREDALEHLALIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHS--QQTPTP--LCQSQCGKLSWADWNMDSLSSGFMYCC 865
L+E+G+ + F + IC D V+ + H + + ++ ++ RP F I G F+ ++ Y+K Q + +LG +D+S S Y A+GH++ F++ Q AT++F+NV PQ+QAFN GNW+ +E VR Y + + +D YTG +G +N G + Y+ +PVPK ++++V + + I +G+N+ H IC ++ D + +W D+L GY Y C V D V ++P DL H + FV S I ++ + G+ SL + C ++R ++ E Q + Y + C+E V R+ R+ G D L+++G+E++ FL+ + +C DE+ T + HV L P S+QR + + +F ++ Y + QQ + ILG + N I+ +Y ARGH+A DFI+ Q AT++FVNV PQ+Q+FN GNW+ +E VR Y +V TG +G+ + G + +L + E+ N IP P+ Y++VV + T + I +G NNPH+ QQ +C+ K+ W W + L G+ Y C
Sbjct: 210 LLEVGFEVSDGFLQTMDICHDEVNEVTRYVHHVLNPSSASYQ-RSVARPRF---IEG--DFY---KGKNVDNLYTKVQQNITISKILG-----------MDASPYFNDSIDIYMARGHMAAKTDFIFGSPQRATFFFVNVGPQWQAFNAGNWERVEDGVRKYVVDQQLTVDCYTGIWGVATLPDVN--GEQRELYLAFDE-NNNGLIPVPKIYFRIVIDRASRKGIVLIGVNNPHASLEQIMQEYVICEDIGDYIDWINWKKDDLIKGYSYACSVPDFIKVVKHLPLDDLETLGVLGVSEXIWQHWWNLIFVIKFSSTINEIVIIRFDPSGVVELKSGE-SLDMYCSESFVGAFENRTKVSAECQSGFTYLVGGQKFNFSDFSCTEWPVYTARRTGRNC---NGGTD---------LLEVGFEVSDGFLQTMDICHDEVNEVTRYVHHV----------LNPSSASYQRSVARPRFIEGDFYKGKNVDNLYTKVQQNITISKILGMDASPYFNDSID------IYMARGHMAAKTDFIFGAPQKATFFFVNVGPQWQAFNGGNWERVEDGVRKYVAKENLTVDCYTGIWGVSTLPDVNGAQQELYLAFDENNNGLIPVPKIYFRVVIDRATRKGIVLIGVNNPHATLQQIKEEYVICEDIGDKIDWIQWAKEDLYKGYSYAC 839 HSP 2 Score: 209.149 bits (531), Expect = 3.629e-51 Identity = 186/723 (25.73%), Postives = 304/723 (42.05%), Query Frame = 0
Query: 242 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSS----SSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVK---------------------------------------DLRD---------TVDY-------VPDLIHGSQVEFVCHGSEEIPSLIRDGQPSLLLTCDDRRFLTPEAQGTYVYTLKCSEPQVPKILRSPRSACAAQGADDREDALEHLALIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALE--GAVRDYAESLKASVKVITGTYGLLRFKNTIG-------------------KLRPFFLYTEDGNQ-WIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ----QTPTPLCQSQCGKLSWADW---------NMDSLSSGFMYCCR 866
L+E+G+ + F + IC D V+ + H + + ++ ++ RP F I G F+ ++ Y+K Q + +LG +D+S S Y A+GH++ F++ Q AT++F+NV PQ+QAFN GNW+ +E VR Y +D YTG +G +N G+ + Y+ +PVPK +++VV + T I +G+N+ H + IC ++ D++ W ++L GY Y C V DL+D T+++ V +L G ++ C S P Q L C E Q CS V R+ RS G D L+++G++++ FL+ + +C DE+ T + HV+ +S N + RPSF+ PG +F ++ Q Y + QQ + ILG ++ + E +++Y ARGH+A DFI+ Q AT+YFVN APQ+Q+FN GNW+ +E AVR YA + TGT+G++R + R F+L +D N +P P Y++++ N N+ I VG NNPH+ + +C +++W W + +L G++Y C
Sbjct: 590 LLEVGFEVSDGFLQTMDICHDEVNEVTRYVHHVLNPSSASYQ-RSVARPRF---IEG--DFY---KGKNVDNLYTKVQQNITISKILG-----------MDASPYFNDSIDIYMARGHMAAKTDFIFGAPQKATFFFVNVGPQWQAFNGGNWERVEDGVRKYVAKENLTVDCYTGIWGVSTLPDVN--GAQQELYLAFDE-NNNGLIPVPKIYFRVVIDRATRKGIVLIGVNNPHATLQQIKEEYVICEDIGDKIDWIQWAKEDLYKGYSYACSVPDFIKVVKHLPLEDLETSGILGITQDPDVADEGCSFRVTGDLKDPAPLFANFGTLEWLEPNSSGVVELKSGDAIDMYCSKSFVAPFT---NQVKLSAQCLGGFTYLVEGQKYNFSDFSCSAWPVYTARRTGRSC---NGGTD---------LLEVGFDVSEGFLKTMDICHDEINEVTRYVHHVLTPSS-NGYQRSVNRPSFK---PG------DFYAGKNVDQLYTQVQQNQTISNILG----MDASPYFEG---SNIYLARGHMAAKVDFIFGSPQRATFYFVNAAPQWQAFNAGNWERVEDGDAVRKYAAKSGQRILCYTGTWGIMRLPAPQSATNTNTTTATTTNVSAFPPQQRDFYLARDDNNNGLLPVPMLYYRLLVNSXRNQGIVLVGVNNPHASLNEISSEYVICDDIQEQVAWLRWMNNNNNNNKDRRNLKKGYLYACH 1257 HSP 3 Score: 130.568 bits (327), Expect = 8.473e-27 Identity = 73/223 (32.74%), Postives = 112/223 (50.22%), Query Frame = 0
Query: 10 LTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHS--QQTPTP--LCQSQCGKLSWADWNMDSLSSGFMYCC 227
L P S+QR + + +F ++ Y + QQ + ILG + N I+ +Y ARGH+A DFI+ Q AT++FVNV PQ+Q+FN GNW+ +E VR Y +V TG +G+ + G + +L + E+ N IP P+ Y++VV + T + I +G NNPH+ QQ +C+ K+ W W + L G+ Y C
Sbjct: 623 LNPSSASYQRSVARPRFIEGDFYKGKNVDNLYTKVQQNITISKILGMDASPYFNDSID------IYMARGHMAAKTDFIFGAPQKATFFFVNVGPQWQAFNGGNWERVEDGVRKYVAKENLTVDCYTGIWGVSTLPDVNGAQQELYLAFDENNNGLIPVPKIYFRVVIDRATRKGIVLIGVNNPHATLQQIKEEYVICEDIGDKIDWIQWAKEDLYKGYSYAC 839 HSP 4 Score: 129.413 bits (324), Expect = 1.920e-26 Identity = 70/223 (31.39%), Postives = 112/223 (50.22%), Query Frame = 0
Query: 10 LTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ----QTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC 227
L P S+QR + + +F ++ Y + QQ + ILG + N I+ +Y ARGH+A DFI+ Q AT++FVNV PQ+Q+FN GNW+ +E VR Y + +V TG +G+ + G+ R +L + E+ N IP P+ Y+++V + + + I +G NNPH+ +C+ + W +W D L G+ Y C
Sbjct: 243 LNPSSASYQRSVARPRFIEGDFYKGKNVDNLYTKVQQNITISKILGMDASPYFNDSID------IYMARGHMAAKTDFIFGSPQRATFFFVNVGPQWQAFNAGNWERVEDGVRKYVVDQQLTVDCYTGIWGVATLPDVNGEQRELYLAFDENNNGLIPVPKIYFRIVIDRASRKGIVLIGVNNPHASLEQIMQEYVICEDIGDYIDWINWKKDDLIKGYSYAC 459 HSP 5 Score: 120.168 bits (300), Expect = 1.342e-23 Identity = 79/286 (27.62%), Postives = 134/286 (46.85%), Query Frame = 0
Query: 880 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSS----SSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVP 1157
L+E+G+ + F + IC D V+ + H + + ++ ++ RP F I G F+ ++ Y+K Q + +LG +D+S S Y A+GH++ F++ Q AT++F+NV PQ+QAFN GNW+ +E VR Y +D YTG +G +N G+ + Y+ +PVPK +++VV + T I +G+N+ H + IC ++ D++ W ++L GY Y C V D V ++P
Sbjct: 590 LLEVGFEVSDGFLQTMDICHDEVNEVTRYVHHVLNPSSASYQ-RSVARPRF---IEG--DFY---KGKNVDNLYTKVQQNITISKILG-----------MDASPYFNDSIDIYMARGHMAAKTDFIFGAPQKATFFFVNVGPQWQAFNGGNWERVEDGVRKYVAKENLTVDCYTGIWGVSTLPDVN--GAQQELYLAFDE-NNNGLIPVPKIYFRVVIDRATRKGIVLIGVNNPHATLQQIKEEYVICEDIGDKIDWIQWAKEDLYKGYSYACSVPDFIKVVKHLP 852 HSP 6 Score: 119.783 bits (299), Expect = 1.646e-23 Identity = 78/286 (27.27%), Postives = 134/286 (46.85%), Query Frame = 0
Query: 880 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSS----SSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVP 1157
L+E+G+ + F + IC D V+ + H + + ++ ++ RP F I G F+ ++ Y+K Q + +LG +D+S S Y A+GH++ F++ Q AT++F+NV PQ+QAFN GNW+ +E VR Y + + +D YTG +G +N G + Y+ +PVPK ++++V + + I +G+N+ H IC ++ D + +W D+L GY Y C V D V ++P
Sbjct: 210 LLEVGFEVSDGFLQTMDICHDEVNEVTRYVHHVLNPSSASYQ-RSVARPRF---IEG--DFY---KGKNVDNLYTKVQQNITISKILG-----------MDASPYFNDSIDIYMARGHMAAKTDFIFGSPQRATFFFVNVGPQWQAFNAGNWERVEDGVRKYVVDQQLTVDCYTGIWGVATLPDVN--GEQRELYLAFDE-NNNGLIPVPKIYFRIVIDRASRKGIVLIGVNNPHASLEQIMQEYVICEDIGDYIDWINWKKDDLIKGYSYACSVPDFIKVVKHLP 472 HSP 7 Score: 111.694 bits (278), Expect = 5.218e-21 Identity = 88/335 (26.27%), Postives = 152/335 (45.37%), Query Frame = 0
Query: 228 RSINGRSISPKDLALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSS---SSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALE--GSVRDYAISSKGAIDIYTGTYGYL----------------FYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDF----TPSRICSNVCDEVPRADW---------DMDNLDSGYMYCCKVKDLRDTVDYVP-DLIHGSQV 527
RS NG + L+E+G+ + F + IC D V+ + H + + ++ ++ +RPSF+ F+ +++ Y++ Q + + +LG +D+S S Y A+GH++ F++ Q AT+YF+N APQ+QAFN GNW+ +E +VR YA S I YTGT+G + +++ +R + LPVP +++++ N N I VG+N+ H + IC ++ ++V W D NL GY+Y C V D V ++P DL++ S++
Sbjct: 970 RSCNGGT------DLLEVGFDVSEGFLKTMDICHDEINEVTRYVHHVLTPSSNGYQ-RSVNRPSFKPG-----DFY---AGKNVDQLYTQVQQNQTISNILG-----------MDASPYFEGSNIYLARGHMAAKVDFIFGSPQRATFYFVNAAPQWQAFNAGNWERVEDGDAVRKYAAKSGQRILCYTGTWGIMRLPAPQSATNTNTTTATTTNVSAFPPQQRDFYLARDDNNNGLLPVPMLYYRLLVNSXRNQGIVLVGVNNPHASLNEISSEYVICDDIQEQVAWLRWMNNNNNNNKDRRNLKKGYLYACHVGDFVKAVGHLPSDLLNVSEI 1278 HSP 8 Score: 109.383 bits (272), Expect = 3.191e-20 Identity = 85/326 (26.07%), Postives = 145/326 (44.48%), Query Frame = 0
Query: 866 RSINGRSISPKDLALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSS---SSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALE--GSVRDYAISSKGAIDIYTGTYGYL----------------FYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDF----TPSRICSNVCDEVPRADW---------DMDNLDSGYMYCCKVKDLRDTVDYVP 1157
RS NG + L+E+G+ + F + IC D V+ + H + + ++ ++ +RPSF+ F+ +++ Y++ Q + + +LG +D+S S Y A+GH++ F++ Q AT+YF+N APQ+QAFN GNW+ +E +VR YA S I YTGT+G + +++ +R + LPVP +++++ N N I VG+N+ H + IC ++ ++V W D NL GY+Y C V D V ++P
Sbjct: 970 RSCNGGT------DLLEVGFDVSEGFLKTMDICHDEINEVTRYVHHVLTPSSNGYQ-RSVNRPSFKPG-----DFY---AGKNVDQLYTQVQQNQTISNILG-----------MDASPYFEGSNIYLARGHMAAKVDFIFGSPQRATFYFVNAAPQWQAFNAGNWERVEDGDAVRKYAAKSGQRILCYTGTWGIMRLPAPQSATNTNTTTATTTNVSAFPPQQRDFYLARDDNNNGLLPVPMLYYRLLVNSXRNQGIVLVGVNNPHASLNEISSEYVICDDIQEQVAWLRWMNNNNNNNKDRRNLKKGYLYACHVGDFVKAVGHLP 1269 HSP 9 Score: 108.612 bits (270), Expect = 4.297e-20 Identity = 74/254 (29.13%), Postives = 118/254 (46.46%), Query Frame = 0
Query: 10 LTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALE--GAVRDYAESLKASVKVITGTYGLLRFKNTIG-------------------KLRPFFLYTEDGNQ-WIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ----QTPTPLCQSQCGKLSWADW---------NMDSLSSGFMYCCR 228
LTP +QR + + +F ++ Q Y + QQ + ILG ++ + E +++Y ARGH+A DFI+ Q AT+YFVN APQ+Q+FN GNW+ +E AVR YA + TGT+G++R + R F+L +D N +P P Y++++ N N+ I VG NNPH+ + +C +++W W + +L G++Y C
Sbjct: 1011 LTPSSNGYQRSVNRPSFKPGDFYAGKNVDQLYTQVQQNQTISNILG----MDASPYFEG---SNIYLARGHMAAKVDFIFGSPQRATFYFVNAAPQWQAFNAGNWERVEDGDAVRKYAAKSGQRILCYTGTWGIMRLPAPQSATNTNTTTATTTNVSAFPPQQRDFYLARDDNNNGLLPVPMLYYRLLVNSXRNQGIVLVGVNNPHASLNEISSEYVICDDIQEQVAWLRWMNNNNNNNKDRRNLKKGYLYACH 1257
BLAST of duplex-specific nuclease vs. nr
Match: gi|1321328053|ref|XP_023306795.1| (uncharacterized protein LOC111688508 [Lucilia cuprina]) HSP 1 Score: 242.276 bits (617), Expect = 1.276e-61 Identity = 187/671 (27.87%), Postives = 303/671 (45.16%), Query Frame = 0
Query: 242 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSS----SSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKD-LRDTVDYVPDLIHGSQV--------EFVCHG--SEEIPSLIRDG-------QPSLLLTCD---------DRRFLTPEAQGTYV--YTLKCSEPQVP-KILRSPRSACAA----QGADDREDALEHLALIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ----QTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC 865
L+E+G+ + G F + IC D V+ + H V + ++ RP F + G F+ ++ Y+K Q K L +LG +D+S+ S + A+GH+ F++ Q AT+ F+NVAPQ+QAFN GNW+ +E VR Y + +D YTGT+G +N G+ K Y+ +PVPK +++VV + T I +G+N+ H + IC ++ D++ WD ++L GY Y C V D LR D + + + V F G + P + G QP+ L+ F+ P T V Y LK S V K +C+A L+++G++++ FL+ + +C DE+ T + HV L P ++QR + + +F ++ Y + QQ + +ILG + N S +LY ARGH+A DFI+ Q +T++FVNVAPQ+Q+FN GNW+ +E VR Y + +V TGT+G+ + G + +L + E+ N IP P+ Y++VV + T + I +G NNPH+ Q +C ++ W +W+ + G+ Y C
Sbjct: 216 LLEVGFEVSGGFIQTMDICHDEINEVTRYVHH-VLNPSSSVYQRSVARPRF---VEG--DFY---AGKNVDNLYTKVQQNKTLSCILG-----------MDASAYFNDSIDIFMARGHMGAKTDFIFGSPQRATFLFVNVAPQWQAFNGGNWERVEDGVRKYVSNKALTVDCYTGTWGISTLPDVN--GTQKELYLSFDE-NNNGLIPVPKIYYRVVIDRVTRQGIVLIGVNNPHATLQQVKEEYIICEDIGDKIDWLQWDKNDLHKGYSYACSVPDFLRVVKDLPVESLETTGVLGINEVGCSFRVSGDLKDPAPLFAKAGVYDWLVPQPNGLVELSYGEAIDMYCSNSFVKPFENHTKVTAYCLKGSNYLVTNKYYNFSNFSCSAWPTYTARRTGRQCNGGTDLLEVGFKVSQGFLQTMDICHDEINEVTRYVHHV----------LNPSSSAYQRSVTRPRFVEGDFYAGKNVDNLYTKVQQNKTISSILGMDASPYFND------SINLYMARGHMAAKIDFIFASPQRSTFFFVNVAPQWQAFNAGNWERVEDGVRKYVSNNALTVDCYTGTWGVSTLPDVNGTHQELYLAFDENNNGLIPVPKIYFRVVIDRATLQGIVLIGVNNPHASLQQIQKEYIVCNDISHQIDWINWSKKDIIKGYSYAC 847 HSP 2 Score: 150.214 bits (378), Expect = 7.845e-33 Identity = 93/318 (29.25%), Postives = 155/318 (48.74%), Query Frame = 0
Query: 605 LIQIGWEINGVFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHS--QQTPTP--LCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISPKDL---ALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVY 914
L+++G+E++G F++ + +C DE+ T + HV L P +QR + + +F ++ Y + QQ + ILG + N I+ ++ ARGH+ DFI+ Q AT+ FVNVAPQ+Q+FN GNW+ +E VR Y + +V TGT+G+ + G + +L + E+ N IP P+ Y++VV + T + I +G NNPH+ QQ +C+ K+ W W+ + L G+ Y C + + KDL +L G + + ++ D + L+ K VY
Sbjct: 216 LLEVGFEVSGGFIQTMDICHDEINEVTRYVHHV----------LNPSSSVYQRSVARPRFVEGDFYAGKNVDNLYTKVQQNKTLSCILGMDASAYFNDSID------IFMARGHMGAKTDFIFGSPQRATFLFVNVAPQWQAFNGGNWERVEDGVRKYVSNKALTVDCYTGTWGISTLPDVNGTQKELYLSFDENNNGLIPVPKIYYRVVIDRVTRQGIVLIGVNNPHATLQQVKEEYIICEDIGDKIDWLQWDKNDLHKGYSYACSVPDFLRVV-KDLPVESLETTGVLGINEVGCSFRVSGDLKDPAPLFAKAGVY 516 HSP 3 Score: 135.961 bits (341), Expect = 1.947e-28 Identity = 95/281 (33.81%), Postives = 145/281 (51.60%), Query Frame = 0
Query: 605 LIQIGWEING-VFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRF----------KNTIGKLRPFFLYTEDGNQWI-PAPEYYWKVV--HNEQTNEAIAFVGHNNPHSQ----QTPTPLCQSQCGKLSWADWNMD-SLSSGFMYCCR 866
+ +IG+ I G FLE +++C D+ L TL+ +H ++ AS++ + +R +F + GH F N LY Y+ QQ A + + GQ+E + N +L+ ARGHLA +DFIY Q AT+ F NVAPQ+Q+FN G W ALE VR + K V TGT+G+L+ ++ + R F+L + N I P P+ Y++V+ + IA VG NNPH+ + +C ++SW W + +L GFMY C+
Sbjct: 1069 MYRIGFNITGNRFLETMRICYDDTLLRTLYVQHTLLPASVHFQK-NVKRLNFSK--AGH---FKNV-NMNQLYTQQYQRQQAAGL--LQGQHEHLFDNN--------TLFLARGHLAAKSDFIYGNLQRATFNFFNVAPQWQAFNGGQWAALEDEVRKFVAKSKLMVDCYTGTFGVLQLPYKTGGSGGGAASVVQYRDFYLTRDSNNNGILPVPQLYYRVLIDRSRYPMRGIALVGVNNPHATLNEINSSYIICDDIHDQVSWLRWMRNKNLKKGFMYACQ 1332 HSP 4 Score: 130.568 bits (327), Expect = 9.395e-27 Identity = 82/275 (29.82%), Postives = 132/275 (48.00%), Query Frame = 0
Query: 10 LTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHS--QQTPTP--LCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISPKDL---ALVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVY 276
L P +QR + + +F ++ Y + QQ + ILG + N I+ ++ ARGH+ DFI+ Q AT+ FVNVAPQ+Q+FN GNW+ +E VR Y + +V TGT+G+ + G + +L + E+ N IP P+ Y++VV + T + I +G NNPH+ QQ +C+ K+ W W+ + L G+ Y C + + KDL +L G + + ++ D + L+ K VY
Sbjct: 249 LNPSSSVYQRSVARPRFVEGDFYAGKNVDNLYTKVQQNKTLSCILGMDASAYFNDSID------IFMARGHMGAKTDFIFGSPQRATFLFVNVAPQWQAFNGGNWERVEDGVRKYVSNKALTVDCYTGTWGISTLPDVNGTQKELYLSFDENNNGLIPVPKIYYRVVIDRVTRQGIVLIGVNNPHATLQQVKEEYIICEDIGDKIDWLQWDKNDLHKGYSYACSVPDFLRVV-KDLPVESLETTGVLGINEVGCSFRVSGDLKDPAPLFAKAGVY 516 HSP 5 Score: 129.413 bits (324), Expect = 2.005e-26 Identity = 85/286 (29.72%), Postives = 136/286 (47.55%), Query Frame = 0
Query: 880 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSS----SSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVP 1157
L+E+G+ + G F + IC D V+ + H V + ++ RP F + G F+ ++ Y+K Q K L +LG +D+S+ S + A+GH+ F++ Q AT+ F+NVAPQ+QAFN GNW+ +E VR Y + +D YTGT+G +N G+ K Y+ +PVPK +++VV + T I +G+N+ H + IC ++ D++ WD ++L GY Y C V D V +P
Sbjct: 216 LLEVGFEVSGGFIQTMDICHDEINEVTRYVHH-VLNPSSSVYQRSVARPRF---VEG--DFY---AGKNVDNLYTKVQQNKTLSCILG-----------MDASAYFNDSIDIFMARGHMGAKTDFIFGSPQRATFLFVNVAPQWQAFNGGNWERVEDGVRKYVSNKALTVDCYTGTWGISTLPDVN--GTQKELYLSFDE-NNNGLIPVPKIYYRVVIDRVTRQGIVLIGVNNPHATLQQVKEEYIICEDIGDKIDWLQWDKNDLHKGYSYACSVPDFLRVVKDLP 478 HSP 6 Score: 128.257 bits (321), Expect = 4.778e-26 Identity = 71/223 (31.84%), Postives = 115/223 (51.57%), Query Frame = 0
Query: 10 LTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFL-YTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ----QTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC 227
L P ++QR + + +F ++ Y + QQ + +ILG + N S +LY ARGH+A DFI+ Q +T++FVNVAPQ+Q+FN GNW+ +E VR Y + +V TGT+G+ + G + +L + E+ N IP P+ Y++VV + T + I +G NNPH+ Q +C ++ W +W+ + G+ Y C
Sbjct: 631 LNPSSSAYQRSVTRPRFVEGDFYAGKNVDNLYTKVQQNKTISSILGMDASPYFND------SINLYMARGHMAAKIDFIFASPQRSTFFFVNVAPQWQAFNAGNWERVEDGVRKYVSNNALTVDCYTGTWGVSTLPDVNGTHQELYLAFDENNNGLIPVPKIYFRVVIDRATLQGIVLIGVNNPHASLQQIQKEYIVCNDISHQIDWINWSKKDIIKGYSYAC 847 HSP 7 Score: 126.716 bits (317), Expect = 1.305e-25 Identity = 88/328 (26.83%), Postives = 158/328 (48.17%), Query Frame = 0
Query: 242 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSS----SSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVP-DLIHGSQVEFVCHGSEEIPSLIRDGQPSLLLTCDDRRFLTP 560
L+E+G+ + F + IC D V+ + H + + ++ ++ RP F + G F+ ++ Y+K Q K + +LG +D+S S Y A+GH++ F++ Q +T++F+NVAPQ+QAFN GNW+ +E VR Y ++ +D YTGT+G +N G+ + Y+ +PVPK +++VV + T I +G+N+ H + +C+++ ++ +W ++ GY Y C V D +TV +P D + + V + EE P +I L++ + RR + P
Sbjct: 598 LLEVGFKVSQGFLQTMDICHDEINEVTRYVHHVLNPSSSAYQ-RSVTRPRF---VEG--DFY---AGKNVDNLYTKVQQNKTISSILG-----------MDASPYFNDSINLYMARGHMAAKIDFIFASPQRSTFFFVNVAPQWQAFNAGNWERVEDGVRKYVSNNALTVDCYTGTWGVSTLPDVN--GTHQELYLAFDE-NNNGLIPVPKIYFRVVIDRATLQGIVLIGVNNPHASLQQIQKEYIVCNDISHQIDWINWSKKDIIKGYSYACTVPDFLETVKDLPLDQLQTTGVLGLSKLPEEDPLIILTLSSVLIVEVECRRAIQP 902 HSP 8 Score: 120.939 bits (302), Expect = 8.508e-24 Identity = 82/295 (27.80%), Postives = 144/295 (48.81%), Query Frame = 0
Query: 880 LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSS----SSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVP-DLSQGTGA 1165
L+E+G+ + F + IC D V+ + H + + ++ ++ RP F + G F+ ++ Y+K Q K + +LG +D+S S Y A+GH++ F++ Q +T++F+NVAPQ+QAFN GNW+ +E VR Y ++ +D YTGT+G +N G+ + Y+ +PVPK +++VV + T I +G+N+ H + +C+++ ++ +W ++ GY Y C V D +TV +P D Q TG
Sbjct: 598 LLEVGFKVSQGFLQTMDICHDEINEVTRYVHHVLNPSSSAYQ-RSVTRPRF---VEG--DFY---AGKNVDNLYTKVQQNKTISSILG-----------MDASPYFNDSINLYMARGHMAAKIDFIFASPQRSTFFFVNVAPQWQAFNAGNWERVEDGVRKYVSNNALTVDCYTGTWGVSTLPDVN--GTHQELYLAFDE-NNNGLIPVPKIYFRVVIDRATLQGIVLIGVNNPHASLQQIQKEYIVCNDISHQIDWINWSKKDIIKGYSYACTVPDFLETVKDLPLDQLQTTGV 869 HSP 9 Score: 118.242 bits (295), Expect = 6.119e-23 Identity = 87/293 (29.69%), Postives = 144/293 (49.15%), Query Frame = 0
Query: 242 LVEIGWILDG-KFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMR--FGTKD-----YLPVPKYFWKVV--HNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMD-NLDSGYMYCCKVKDLRDTVDYVP 519
+ IG+ + G +F ++IC D +L+ +H++ ++ F+ K R +F + F +V +N+ Y+++ Q ++ +L G E + D++ T + A+GHL+ + F+Y Q AT+ F NVAPQ+QAFN G W ALE VR + SK +D YTGT+G L + R + T+D LPVP+ +++V+ + IA VG+N+ H IC ++ D+V W + NL G+MY C+V D V ++P
Sbjct: 1069 MYRIGFNITGNRFLETMRICYDDTLLRTLYVQHTLLPASVHFQ-KNVKRLNFSK-----AGHFKNVN---MNQLYTQQYQRQQAAGLLQGQH-----EHLFDNN---TLFLARGHLAAKSDFIYGNLQRATFNFFNVAPQWQAFNGGQWAALEDEVRKFVAKSKLMVDCYTGTFGVLQLPYKTGGSGGGAASVVQYRDFYLTRDSNNNGILPVPQLYYRVLIDRSRYPMRGIALVGVNNPHATLNEINSSYIICDDIHDQVSWLRWMRNKNLKKGFMYACQVPDFVAAVGHLP 1344 HSP 10 Score: 118.242 bits (295), Expect = 6.119e-23 Identity = 87/293 (29.69%), Postives = 144/293 (49.15%), Query Frame = 0
Query: 880 LVEIGWILDG-KFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMR--FGTKD-----YLPVPKYFWKVV--HNPQTNHAIAFVGMNDAHLDFTPSR----ICSNVCDEVPRADWDMD-NLDSGYMYCCKVKDLRDTVDYVP 1157
+ IG+ + G +F ++IC D +L+ +H++ ++ F+ K R +F + F +V +N+ Y+++ Q ++ +L G E + D++ T + A+GHL+ + F+Y Q AT+ F NVAPQ+QAFN G W ALE VR + SK +D YTGT+G L + R + T+D LPVP+ +++V+ + IA VG+N+ H IC ++ D+V W + NL G+MY C+V D V ++P
Sbjct: 1069 MYRIGFNITGNRFLETMRICYDDTLLRTLYVQHTLLPASVHFQ-KNVKRLNFSK-----AGHFKNVN---MNQLYTQQYQRQQAAGLLQGQH-----EHLFDNN---TLFLARGHLAAKSDFIYGNLQRATFNFFNVAPQWQAFNGGQWAALEDEVRKFVAKSKLMVDCYTGTFGVLQLPYKTGGSGGGAASVVQYRDFYLTRDSNNNGILPVPQLYYRVLIDRSRYPMRGIALVGVNNPHATLNEINSSYIICDDIHDQVSWLRWMRNKNLKKGFMYACQVPDFVAAVGHLP 1344 HSP 11 Score: 115.546 bits (288), Expect = 3.710e-22 Identity = 79/238 (33.19%), Postives = 117/238 (49.16%), Query Frame = 0
Query: 10 LTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETIL-GQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRF----------KNTIGKLRPFFLYTEDGNQWI-PAPEYYWKVV--HNEQTNEAIAFVGHNNPHSQ----QTPTPLCQSQCGKLSWADWNMD-SLSSGFMYCCR 228
L P FQ+++ N A + ++ Q Y + Q Q +L GQ+E + N +L+ ARGHLA +DFIY Q AT+ F NVAPQ+Q+FN G W ALE VR + K V TGT+G+L+ ++ + R F+L + N I P P+ Y++V+ + IA VG NNPH+ + +C ++SW W + +L GFMY C+
Sbjct: 1103 LLPASVHFQKNVKRLNFSKAGHFKNVNMNQLYTQQYQRQQAAGLLQGQHEHLFDNN--------TLFLARGHLAAKSDFIYGNLQRATFNFFNVAPQWQAFNGGQWAALEDEVRKFVAKSKLMVDCYTGTFGVLQLPYKTGGSGGGAASVVQYRDFYLTRDSNNNGILPVPQLYYRVLIDRSRYPMRGIALVGVNNPHATLNEINSSYIICDDIHDQVSWLRWMRNKNLKKGFMYACQ 1332
BLAST of duplex-specific nuclease vs. nr
Match: gi|930659373|gb|KPJ01440.1| (hypothetical protein RR46_08477 [Papilio xuthus]) HSP 1 Score: 229.565 bits (584), Expect = 1.571e-61 Identity = 149/481 (30.98%), Postives = 235/481 (48.86%), Query Frame = 0
Query: 700 GNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTE-DGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPH---SQQTPTPLCQSQC---GKLSWADWNMDSLSSGFMYCCRSINGRSISPKDLA-----LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKD-YLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRAD-------WDMDNLDSGYMYCCKVKDLRDTVDYVPDLS 1160
G + E +++ Y ARGHLA DFIY Q AT+YF+N APQ+Q FN GNW LE +R + + V TGTYG+L+ +++ +L +L+ + + N +P P YY+KVV+++ AFVG NNP+ + C C +W W D + G+ +CC + R+ P + L+ +G+ ++ F C D R L+ + RPS+R F+P V +N Y+ Q + +G + + +ST Y A+GHL+ F+Y Q AT+YF+N APQ+Q FN GNW LE ++R ++ +YTGTYG L +L D +++ I L R + LPVP Y++KVV++ AFVG+N+ ++ +R C++ R++ W D +D GY +CC ++D R V ++P +
Sbjct: 34 GTDLAEKYVTSTQYLARGHLAAKTDFIYATGQRATFYFINCAPQWQQFNGGNWNLLEQNLRIRIGAAGYNTVVYTGTYGILQLRDSNNQLSSIYLHRDSNNNTQLPVPLYYYKVVYDQAKRLGTAFVGINNPYITLEEARSLQFCNDHCRSNSSFTWLSWQPDRVDRGYSFCCSIEDFRTFVPHLPSFTVNDLLTVGFTVNNVFYPLYWSCFDQNRLEVLYVWYEQ-NPPNAVNQVGVSRPSWRAGT-----FYPGVD---VNNRYTVAQQKVTVSNYVGTD--------LAEKYVTSTQYLARGHLAAKTDFIYATGQRATFYFINCAPQWQQFNGGNWNLLEQNLRIRIGAAGYNTVVYTGTYGIL---QL-RDSNNQLVDIYLHRDSNNNTQLPVPLYYYKVVYDQAKRLGTAFVGINNPYITLEEARTLQ-FCNDHCRSNSSFTWLSWQPDRVDRGYSFCCSIEDFRTFVPHLPSFT 492 HSP 2 Score: 229.18 bits (583), Expect = 2.377e-61 Identity = 149/480 (31.04%), Postives = 234/480 (48.75%), Query Frame = 0
Query: 62 GNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTE-DGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPH---SQQTPTPLCQSQC---GKLSWADWNMDSLSSGFMYCCRSINGRSISPKDLA-----LVEIGWILDGKFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKD-YLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRAD-------WDMDNLDSGYMYCCKVKDLRDTVDYVPDL 521
G + E +++ Y ARGHLA DFIY Q AT+YF+N APQ+Q FN GNW LE +R + + V TGTYG+L+ +++ +L +L+ + + N +P P YY+KVV+++ AFVG NNP+ + C C +W W D + G+ +CC + R+ P + L+ +G+ ++ F C D R L+ + RPS+R F+P V +N Y+ Q + +G + + +ST Y A+GHL+ F+Y Q AT+YF+N APQ+Q FN GNW LE ++R ++ +YTGTYG L +L D +++ I L R + LPVP Y++KVV++ AFVG+N+ ++ +R C++ R++ W D +D GY +CC ++D R V ++P
Sbjct: 34 GTDLAEKYVTSTQYLARGHLAAKTDFIYATGQRATFYFINCAPQWQQFNGGNWNLLEQNLRIRIGAAGYNTVVYTGTYGILQLRDSNNQLSSIYLHRDSNNNTQLPVPLYYYKVVYDQAKRLGTAFVGINNPYITLEEARSLQFCNDHCRSNSSFTWLSWQPDRVDRGYSFCCSIEDFRTFVPHLPSFTVNDLLTVGFTVNNVFYPLYWSCFDQNRLEVLYVWYEQ-NPPNAVNQVGVSRPSWRAGT-----FYPGVD---VNNRYTVAQQKVTVSNYVGTD--------LAEKYVTSTQYLARGHLAAKTDFIYATGQRATFYFINCAPQWQQFNGGNWNLLEQNLRIRIGAAGYNTVVYTGTYGIL---QL-RDSNNQLVDIYLHRDSNNNTQLPVPLYYYKVVYDQAKRLGTAFVGINNPYITLEEARTLQ-FCNDHCRSNSSFTWLSWQPDRVDRGYSFCCSIEDFRTFVPHLPSF 491
BLAST of duplex-specific nuclease vs. nr
Match: gi|1133424817|ref|XP_019868161.1| (PREDICTED: uncharacterized protein LOC109596983 [Aethina tumida]) HSP 1 Score: 233.802 bits (595), Expect = 8.828e-61 Identity = 180/673 (26.75%), Postives = 302/673 (44.87%), Query Frame = 0
Query: 243 VEIGWILDG-KFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRR-DITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLP-NGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDY-LPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL-------IHGSQVEFVCHGSE----------------EIPSLIRDGQPSLLL------------TCDDRRFLTPEAQGTYVYTLKCSEPQVPKILRSPRSACAAQGADDREDALEHLALIQIGWEING-VFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKN--TIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSI 873
+EIG+ L+ F ++IC D + L++ + + K I++ R +F+ GL + L+ Y + Q K + LP N + +S Y A+GHL+ FVY QQ AT++++N APQ+Q N NW ++E SVR YA +++ +++YTG YG + L ++ ++K + L R + LP+P +WK+++N T + N+ + + P +C +V D + W DN G Y C K L + Y+PDL + GS E V S E S I G P L TC + + C+ R+ ++ C+ G + I+IG+ +N VF I +C D+LL + L++ + + + I+ RP+F+ F N + + Y R+ Q + + +G V+ Q ++ + + Y +RGHL DF+Y Q AT+++VN APQ+Q+ N NW ++E +VR YA + + + TG YG+ N T + +F + N +P PE YWK+++N QT E + + NNP+ + P P+C+ L+W W+ + G Y C +I +I
Sbjct: 117 IEIGFALNSTAFVRLMRICFDDKLKSPLYSYYQLKAK-INYAQTGVARLNFKEGSFYGL--------DSSLDTLYERNVQ----KTTINNQVGLPANSTQYVHDTSDF--YLARGHLTAKTDFVYASQQKATFWYVNTAPQWQTVNAANWNSIEDSVRAYAFNNEVDLEVYTGVYGV---STLPNEATNKANELYLSRDDNNNVALPIPAVYWKLIYNNNTKENVVLFSANNPY-ESNPGTVCEDVSDSLSWVTWKKDNQTKGISYACSAKGLNKIITYLPDLEYECSINVDGSPAEPVIVDSSTLQYVYPNSFDLDISLESGSTIILGCPGSTLNYGRNTTSIVEATCISGSTFLVNGETVTLSKFYCNNIPTSTARRTGKT-CSNSGVE-----------IEIGFALNSTVFARVITVCFDDLLKSPLYSFYH-LTSKIDYAQTGVSRPNFKEGD------FYNLDGTVD--KLYSRAVQKKTINSQVGL--PVSSTQYVKD--NNNYYLSRGHLTAKGDFVYAHQQKATFWYVNTAPQWQTCNGANWNSIEDSVRRYASNKGLDLDIYTGVYGISNLPNESTDEPVDLYFYLDTNNNVALPIPEVYWKLIYNPQTTEHVVLITVNNPY-ESNPEPICEDVSKSLTWLTWSKNDQMKGISYACSAIGLNAI 744 HSP 2 Score: 132.109 bits (331), Expect = 1.162e-27 Identity = 96/370 (25.95%), Postives = 161/370 (43.51%), Query Frame = 0
Query: 519 PDLIHGSQVEFVCHGSEEIPSLIRDGQPSLL------------------LTCDDRRFLTPEAQGTYVYTLKCSEPQVPKILRSPRSACAAQGADDREDALEHLALIQIGWEINGV-FLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKN-TIGKLRPFFLYTEDGNQ-WIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRS 867
P ++ + +E++ S E+ ++ G +L TC E Q + CS +S S G D +E I+IG+ +N F+ +++C D+ L + L++ + + A IN R +F+ + + +SL Y R+ Q + +G N Q + ++ Y ARGHL DF+Y Q AT+++VN APQ+Q+ N NW ++E +VR YA + + ++V TG YG+ N K +L +D N +P P YWK+++N T E + NNP+ + P +C+ LSW W D+ + G Y C +
Sbjct: 24 PVIVDSNTLEYIYPDSSELDISLKSGSKIILGCPGAVLNYGSSTSNVIEATCISGSTFLVEGQNVSISNPSCS--------KSSTSTARYTGKTCESDGVE----IEIGFALNSTAFVRLMRICFDDKLKSPLYS-YYQLKAKINYAQTGVARLNFKEG--------SFYGLDSSLDTLYERNVQKTTINNQVGL--PANSTQYVHD--TSDFYLARGHLTAKTDFVYASQQKATFWYVNTAPQWQTVNAANWNSIEDSVRAYAFNNEVDLEVYTGVYGVSTLPNEATNKANELYLSRDDNNNVALPIPAVYWKLIYNNNTKENVVLFSANNPY-ESNPGTVCEDVSDSLSWVTWKKDNQTKGISYACSA 367 HSP 3 Score: 124.02 bits (310), Expect = 4.565e-25 Identity = 82/283 (28.98%), Postives = 138/283 (48.76%), Query Frame = 0
Query: 881 VEIGWILDG-KFQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRR-DITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLP-NGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDY-LPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 1159
+EIG+ L+ F ++IC D + L++ + + K I++ R +F+ GL + L+ Y + Q K + LP N + +S Y A+GHL+ FVY QQ AT++++N APQ+Q N NW ++E SVR YA +++ +++YTG YG + L ++ ++K + L R + LP+P +WK+++N T + N+ + + P +C +V D + W DN G Y C K L + Y+PDL
Sbjct: 117 IEIGFALNSTAFVRLMRICFDDKLKSPLYSYYQLKAK-INYAQTGVARLNFKEGSFYGL--------DSSLDTLYERNVQ----KTTINNQVGLPANSTQYVHDTSDF--YLARGHLTAKTDFVYASQQKATFWYVNTAPQWQTVNAANWNSIEDSVRAYAFNNEVDLEVYTGVYG---VSTLPNEATNKANELYLSRDDNNNVALPIPAVYWKLIYNNNTKENVVLFSANNPY-ESNPGTVCEDVSDSLSWVTWKKDNQTKGISYACSAKGLNKIITYLPDL 380 HSP 4 Score: 122.479 bits (306), Expect = 1.449e-24 Identity = 57/163 (34.97%), Postives = 89/163 (54.60%), Query Frame = 0
Query: 75 YFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKN--TIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSI 235
Y +RGHL DF+Y Q AT+++VN APQ+Q+ N NW ++E +VR YA + + + TG YG+ N T + +F + N +P PE YWK+++N QT E + + NNP+ + P P+C+ L+W W+ + G Y C +I +I
Sbjct: 583 YLSRGHLTAKGDFVYAHQQKATFWYVNTAPQWQTCNGANWNSIEDSVRRYASNKGLDLDIYTGVYGISNLPNESTDEPVDLYFYLDTNNNVALPIPEVYWKLIYNPQTTEHVVLITVNNPY-ESNPEPICEDVSKSLTWLTWSKNDQMKGISYACSAIGLNAI 744 HSP 5 Score: 120.553 bits (301), Expect = 5.607e-24 Identity = 84/287 (29.27%), Postives = 140/287 (48.78%), Query Frame = 0
Query: 243 VEIGWILDGK-FQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRR-DITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDL---MRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDLIHG 524
+EIG+ L+ F + +C D L++ + + K ID+ RP+F+ D L D T +L YS+ Q K + +G + + + + D+++ Y ++GHL+ FVY QQ AT++++N APQ+Q N NW ++E SVR YA + +DIYTG YG N S +DL + LP+P+ +WK+++NPQT + + +N+ + + P IC +V + W ++ G Y C L + +PDL +G
Sbjct: 488 IEIGFALNSTVFARVITVCFDDLLKSPLYSFYHLTSK-IDYAQTGVSRPNFKEGDFYNL-----DGTVDKL---YSRAVQKKTINSQVG--LPVSSTQYVKDNNNY---YLSRGHLTAKGDFVYAHQQKATFWYVNTAPQWQTCNGANWNSIEDSVRRYASNKGLDLDIYTGVYGI-----SNLPNESTDEPVDLYFYLDTNNNVALPIPEVYWKLIYNPQTTEHVVLITVNNPY-ESNPEPICEDVSKSLTWLTWSKNDQMKGISYACSAIGLNAIISSIPDLDYG 754 HSP 6 Score: 120.168 bits (300), Expect = 7.932e-24 Identity = 84/287 (29.27%), Postives = 139/287 (48.43%), Query Frame = 0
Query: 881 VEIGWILDGK-FQTQVKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRR-DITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDL---MRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDLSQG 1162
+EIG+ L+ F + +C D L++ + + K ID+ RP+F+ D L D T +L YS+ Q K + +G + + + + D+++ Y ++GHL+ FVY QQ AT++++N APQ+Q N NW ++E SVR YA + +DIYTG YG N S +DL + LP+P+ +WK+++NPQT + + +N+ + + P IC +V + W ++ G Y C L + +PDL G
Sbjct: 488 IEIGFALNSTVFARVITVCFDDLLKSPLYSFYHLTSK-IDYAQTGVSRPNFKEGDFYNL-----DGTVDKL---YSRAVQKKTINSQVG--LPVSSTQYVKDNNNY---YLSRGHLTAKGDFVYAHQQKATFWYVNTAPQWQTCNGANWNSIEDSVRRYASNKGLDLDIYTGVYGI-----SNLPNESTDEPVDLYFYLDTNNNVALPIPEVYWKLIYNPQTTEHVVLITVNNPY-ESNPEPICEDVSKSLTWLTWSKNDQMKGISYACSAIGLNAIISSIPDLDYG 754 HSP 7 Score: 119.783 bits (299), Expect = 9.852e-24 Identity = 65/197 (32.99%), Postives = 100/197 (50.76%), Query Frame = 0
Query: 35 TSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKN-TIGKLRPFFLYTEDGNQ-WIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRS 229
+SL Y R+ Q + +G N Q + ++ Y ARGHL DF+Y Q AT+++VN APQ+Q+ N NW ++E +VR YA + + ++V TG YG+ N K +L +D N +P P YWK+++N T E + NNP+ + P +C+ LSW W D+ + G Y C +
Sbjct: 176 SSLDTLYERNVQKTTINNQVGL--PANSTQYVHD--TSDFYLARGHLTAKTDFVYASQQKATFWYVNTAPQWQTVNAANWNSIEDSVRAYAFNNEVDLEVYTGVYGVSTLPNEATNKANELYLSRDDNNNVALPIPAVYWKLIYNNNTKENVVLFSANNPY-ESNPGTVCEDVSDSLSWVTWKKDNQTKGISYACSA 367
BLAST of duplex-specific nuclease vs. nr
Match: gi|217272469|dbj|BAH02823.1| (duplex-specific nuclease [Paralithodes camtschaticus]) HSP 1 Score: 222.246 bits (565), Expect = 4.736e-60 Identity = 130/354 (36.72%), Postives = 185/354 (52.26%), Query Frame = 0
Query: 524 GSQVEFVCHGSEEIPSLIRDGQPSLLLTCDDRRFLTPEAQGTYVYTLKCSEPQVPKILRSPRSACAAQGADDREDALEHLALIQIGWEING--VFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISP 875
GS + C GSE LI G ++ C L E +++L CS V + +R C DR IG+E G ++ E I +C + + TL+T+HV+ GA+I +D+ RPSF+ F S S+ Y ++ QL M ILG ++ N II+ S LYFA+GH++PDADF+ QDATYYF+N PQ+Q+FNNGNWK LE A RD AES + ++V +G + LL+ + G L +G + +P P WKVV+ E +++A A VG NNPH P+PLC C L+W D+N+D L+ G+ YCC + R P
Sbjct: 68 GSSLTLACSGSE----LINLGMEAVEAKCAGGVMLAIEGTEWEIWSLGCSN-HVKETIRRNLGTCGEADQGDRH---------SIGFEYYGGSIYYELISVCFEPVSETTLYTEHVLHGANIAAKDIETSRPSFKTS----TGFF-----SVSMSTVYSQASQLQLMTDILGDSD--LANNIIDP--SQQLYFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDINGNPVDILLGLSEGKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHITTAPSPLCSDLCSSLTWIDFNLDDLAHGYTYCCAVDDLRQAIP 394 HSP 2 Score: 189.889 bits (481), Expect = 9.317e-49 Identity = 104/265 (39.25%), Postives = 153/265 (57.74%), Query Frame = 0
Query: 257 VKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 521
+ +C + +L+T+H ++G I +D + RPSF+ T FF ++ YS+ SQ++ + +LG S N IID S YFAKGH+SPDA FV +QDATYYF+N PQ+QAFNNGNWK LE + RD A S + +Y+G + L + +N G+ + L K+ +PVP WKVV+ ++ A A VG+N+ H+ PS +CS++C + D+++D+L GY YCC V DLR + Y+PDL
Sbjct: 152 ISVCFEPVSETTLYTEHVLHGANIAAKDIETSRPSFKTS----TGFF----SVSMSTVYSQASQLQLMTDILGDSDLANN---IIDPSQQL--YFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDIN--GNPVDILLGLSE--GKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHITTAPSPLCSDLCSSLTWIDFNLDDLAHGYTYCCAVDDLRQAIPYIPDL 399 HSP 3 Score: 189.889 bits (481), Expect = 9.317e-49 Identity = 104/265 (39.25%), Postives = 153/265 (57.74%), Query Frame = 0
Query: 895 VKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 1159
+ +C + +L+T+H ++G I +D + RPSF+ T FF ++ YS+ SQ++ + +LG S N IID S YFAKGH+SPDA FV +QDATYYF+N PQ+QAFNNGNWK LE + RD A S + +Y+G + L + +N G+ + L K+ +PVP WKVV+ ++ A A VG+N+ H+ PS +CS++C + D+++D+L GY YCC V DLR + Y+PDL
Sbjct: 152 ISVCFEPVSETTLYTEHVLHGANIAAKDIETSRPSFKTS----TGFF----SVSMSTVYSQASQLQLMTDILGDSDLANN---IIDPSQQL--YFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDIN--GNPVDILLGLSE--GKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHITTAPSPLCSDLCSSLTWIDFNLDDLAHGYTYCCAVDDLRQAIPYIPDL 399 HSP 4 Score: 186.808 bits (473), Expect = 8.256e-48 Identity = 98/236 (41.53%), Postives = 136/236 (57.63%), Query Frame = 0
Query: 2 GASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISP 237
GA+I +D+ RPSF+ F S S+ Y ++ QL M ILG ++ N II+ S LYFA+GH++PDADF+ QDATYYF+N PQ+Q+FNNGNWK LE A RD AES + ++V +G + LL+ + G L +G + +P P WKVV+ E +++A A VG NNPH P+PLC C L+W D+N+D L+ G+ YCC + R P
Sbjct: 172 GANIAAKDIETSRPSFKTS----TGFF-----SVSMSTVYSQASQLQLMTDILGDSD--LANNIIDP--SQQLYFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDINGNPVDILLGLSEGKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHITTAPSPLCSDLCSSLTWIDFNLDDLAHGYTYCCAVDDLRQAIP 394
BLAST of duplex-specific nuclease vs. nr
Match: gi|26892281|gb|AAN86143.1| (duplex-specific nuclease [Paralithodes camtschaticus]) HSP 1 Score: 219.55 bits (558), Expect = 4.774e-59 Identity = 130/354 (36.72%), Postives = 183/354 (51.69%), Query Frame = 0
Query: 524 GSQVEFVCHGSEEIPSLIRDGQPSLLLTCDDRRFLTPEAQGTYVYTLKCSEPQVPKILRSPRSACAAQGADDREDALEHLALIQIGWEING--VFLEQIQLCVDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISP 875
GS + C GSE LI G ++ C L E +++L CS V + +R C DR IG+E G ++ E I +C + TL T+HV+ GA+I +D+ RPSF+ F S S+ Y ++ QL M ILG ++ N II+ S LYFA+GH++PDADF+ QDATYYF+N PQ+Q+FNNGNWK LE A RD AES + ++V +G + LL+ + G L +G + +P P WKVV+ E +++A A VG NNPH P+PLC C L+W D+N+D L+ G+ YCC + R P
Sbjct: 68 GSSLTLACSGSE----LINLGMEAVEAKCAGGVMLAIEGTEWEIWSLGCSN-HVKETIRRNLGTCGEADQGDRH---------SIGFEYYGGSIYYELISVCFGPVSETTLRTEHVLHGANIAAKDIETSRPSFKTS----TGFF-----SVSMSTVYSQASQLQLMTDILGDSD--LANNIIDP--SQQLYFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDINGNPVDILLGLSEGKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHITTAPSPLCSDLCSSLTWIDFNLDDLAHGYTYCCAVDDLRQAIP 394 HSP 2 Score: 187.193 bits (474), Expect = 8.100e-48 Identity = 104/265 (39.25%), Postives = 151/265 (56.98%), Query Frame = 0
Query: 257 VKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 521
+ +C +L T+H ++G I +D + RPSF+ T FF ++ YS+ SQ++ + +LG S N IID S YFAKGH+SPDA FV +QDATYYF+N PQ+QAFNNGNWK LE + RD A S + +Y+G + L + +N G+ + L K+ +PVP WKVV+ ++ A A VG+N+ H+ PS +CS++C + D+++D+L GY YCC V DLR + Y+PDL
Sbjct: 152 ISVCFGPVSETTLRTEHVLHGANIAAKDIETSRPSFKTS----TGFF----SVSMSTVYSQASQLQLMTDILGDSDLANN---IIDPSQQL--YFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDIN--GNPVDILLGLSE--GKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHITTAPSPLCSDLCSSLTWIDFNLDDLAHGYTYCCAVDDLRQAIPYIPDL 399 HSP 3 Score: 187.193 bits (474), Expect = 8.100e-48 Identity = 104/265 (39.25%), Postives = 151/265 (56.98%), Query Frame = 0
Query: 895 VKICQDTRRYVSLWTKHSVYGKAIDFRDKTSDRPSFRRDITGLTRFFPDVTQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDYLPVPKYFWKVVHNPQTNHAIAFVGMNDAHLDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 1159
+ +C +L T+H ++G I +D + RPSF+ T FF ++ YS+ SQ++ + +LG S N IID S YFAKGH+SPDA FV +QDATYYF+N PQ+QAFNNGNWK LE + RD A S + +Y+G + L + +N G+ + L K+ +PVP WKVV+ ++ A A VG+N+ H+ PS +CS++C + D+++D+L GY YCC V DLR + Y+PDL
Sbjct: 152 ISVCFGPVSETTLRTEHVLHGANIAAKDIETSRPSFKTS----TGFF----SVSMSTVYSQASQLQLMTDILGDSDLANN---IIDPSQQL--YFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDIN--GNPVDILLGLSE--GKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHITTAPSPLCSDLCSSLTWIDFNLDDLAHGYTYCCAVDDLRQAIPYIPDL 399 HSP 4 Score: 186.808 bits (473), Expect = 8.414e-48 Identity = 98/236 (41.53%), Postives = 136/236 (57.63%), Query Frame = 0
Query: 2 GASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQWIPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQQTPTPLCQSQCGKLSWADWNMDSLSSGFMYCCRSINGRSISP 237
GA+I +D+ RPSF+ F S S+ Y ++ QL M ILG ++ N II+ S LYFA+GH++PDADF+ QDATYYF+N PQ+Q+FNNGNWK LE A RD AES + ++V +G + LL+ + G L +G + +P P WKVV+ E +++A A VG NNPH P+PLC C L+W D+N+D L+ G+ YCC + R P
Sbjct: 172 GANIAAKDIETSRPSFKTS----TGFF-----SVSMSTVYSQASQLQLMTDILGDSD--LANNIIDP--SQQLYFAKGHMSPDADFVTVAEQDATYYFINALPQWQAFNNGNWKYLEYATRDLAESHGSDLRVYSGGWSLLQLDDINGNPVDILLGLSEGKEVVPVPSLTWKVVYEESSSKAAAIVGINNPHITTAPSPLCSDLCSSLTWIDFNLDDLAHGYTYCCAVDDLRQAIP 394
BLAST of duplex-specific nuclease vs. nr
Match: gi|1069816158|ref|XP_018331412.1| (PREDICTED: uncharacterized protein LOC108741209 [Agrilus planipennis]) HSP 1 Score: 227.254 bits (578), Expect = 7.602e-59 Identity = 173/648 (26.70%), Postives = 278/648 (42.90%), Query Frame = 0
Query: 307 TQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDY-LPVPKYFWKVVHNPQTNHAIAFVGMNDAH-LDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPD-----------------------------LIHGSQVEFVCHGSEEIPSLIRDGQPSLLL-----TCDDRRFLTPEAQGTYVYTLKCSEPQVPKILRSPRSACAAQGADDREDALE----------------------------HLAL-------------IQIGWEINGVFLEQIQLCV----DELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESG-----FSTSLYF-ARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQW-IPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ-QTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC 865
T ++ Y + +Q + +LG N ++ + +GH + A F++ Q T+YF+N APQFQ FN GNW LE +VR ++ + + + IYTG +G +N + Y D +Y + VP+ +WKV++ P+ AIAFVG+N+ + L +C+N+C +V W ++ GY YCC V++LR V +P+ LI+ S++ C S + + D QP LL T +D +L ++ GT +++ Q+ + PR G D D H+A I IG+E+ ++ I C ++LY ++G RPS+ + + S+ Y R+ Q + +ILG + SG ST+ YF ARGH A ADF++ T+Y+VN APQFQ+FN GNW LE VR ++ + + + V TG +G+ + GK + +LY + N + I P YWKV+++ +T AIAFVG NNP+ + LC +++W W + + G+ Y C
Sbjct: 79 TNPSVDSLYGRATQRSTINSILG-----LNSNDTRYIQPTNNFFLTRGHFAAKADFIFGAQHRLTFYFVNAAPQFQTFNGGNWNTLENNVRSFSANRQLDLVIYTGGHGVTSLPDVNGNQQDLYLYKD----SNNNYAISVPRLYWKVLYEPENKTAIAFVGVNNPYQLIREKDILCTNICSQVNWLTWKANDNTLGYSYCCDVRELRGNVTNIPNFTVIEGSFPFSKEDDGGSPREDQRQDKGWLINKSKLNLGCAISINVD--LSDPQPLLLKPKRGSTTNDAFYLPDDSSGTINFSVG---QQI--TIACPRRRILVGGTDQGTDTANITCVSAKRFQFGNRNVRFDTITCTNYPFHVARTNGKTCLNGRGREISIGFEVQSRWINHITSCFASSSQDVLYTVFNMTKTILGYQTGF-----PRPSWMDG--------GFYNANQSVDSLYARATQRRTINSILG----------LSSGDTTYIHSTNNYFLARGHFAAKADFVFGAQHRLTFYYVNAAPQFQTFNGGNWNTLESNVRSFSANRQLDLVVYTGGHGVTSLPDVNGKQQDLYLYKDSNNNYAISVPRLYWKVLYDPKTKTAIAFVGVNNPYQLIRRKDVLCTDISSQVNWLTWKANDTTLGYSYSC 687 HSP 2 Score: 141.739 bits (356), Expect = 8.136e-31 Identity = 85/285 (29.82%), Postives = 138/285 (48.42%), Query Frame = 0
Query: 606 IQIGWEINGVFLEQIQLC----VDELLYATLWTKHVVMGASINLRDLTPERPSFQRDIPGHNRLFANFPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQW-IPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ-QTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC--RSINGRSISPKDLALVE 882
I IG+E+ ++ QI C ++LY ++G + F R + P+ SLY R+ Q + + +ILG N N + I+ + + + RGH A ADFI+ T+YFVN APQFQ+FN GNW LE VR ++ + + + + TG +G+ + G + +LY + N + I P YWKV++ + AIAFVG NNP+ + LC + C +++W W + + G+ YCC R + G + + ++E
Sbjct: 23 ISIGFEVQSRWINQITSCFASSTQDVLYTVFNMTKTILGY----------QTGFPRPFWSDGGFYNTNPSVDSLYG---RATQRSTINSILGLNS--NDTRYIQP--TNNFFLTRGHFAAKADFIFGAQHRLTFYFVNAAPQFQTFNGGNWNTLENNVRSFSANRQLDLVIYTGGHGVTSLPDVNGNQQDLYLYKDSNNNYAISVPRLYWKVLYEPENKTAIAFVGVNNPYQLIREKDILCTNICSQVNWLTWKANDNTLGYSYCCDVRELRGNVTNIPNFTVIE 290 HSP 3 Score: 135.576 bits (340), Expect = 9.245e-29 Identity = 72/218 (33.03%), Postives = 117/218 (53.67%), Query Frame = 0
Query: 31 FPTSTSLYQTYYRSQQLAQMETILGQNEDVNGNQIIESGFSTSLYFARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQW-IPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ-QTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC--RSINGRSISPKDLALVE 244
+ T+ S+ Y R+ Q + + +ILG N N + I+ + + + RGH A ADFI+ T+YFVN APQFQ+FN GNW LE VR ++ + + + + TG +G+ + G + +LY + N + I P YWKV++ + AIAFVG NNP+ + LC + C +++W W + + G+ YCC R + G + + ++E
Sbjct: 77 YNTNPSVDSLYGRATQRSTINSILGLNS--NDTRYIQP--TNNFFLTRGHFAAKADFIFGAQHRLTFYFVNAAPQFQTFNGGNWNTLENNVRSFSANRQLDLVIYTGGHGVTSLPDVNGNQQDLYLYKDSNNNYAISVPRLYWKVLYEPENKTAIAFVGVNNPYQLIREKDILCTNICSQVNWLTWKANDNTLGYSYCCDVRELRGNVTNIPNFTVIE 290 HSP 4 Score: 128.642 bits (322), Expect = 1.314e-26 Identity = 69/225 (30.67%), Postives = 116/225 (51.56%), Query Frame = 0
Query: 945 TQTRLNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDY-LPVPKYFWKVVHNPQTNHAIAFVGMNDAH-LDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDLSQGTGAWP 1167
T ++ Y + +Q + +LG N ++ + +GH + A F++ Q T+YF+N APQFQ FN GNW LE +VR ++ + + + IYTG +G +N + Y D +Y + VP+ +WKV++ P+ AIAFVG+N+ + L +C+N+C +V W ++ GY YCC V++LR V +P+ + G++P
Sbjct: 79 TNPSVDSLYGRATQRSTINSILG-----LNSNDTRYIQPTNNFFLTRGHFAAKADFIFGAQHRLTFYFVNAAPQFQTFNGGNWNTLENNVRSFSANRQLDLVIYTGGHGVTSLPDVNGNQQDLYLYKD----SNNNYAISVPRLYWKVLYEPENKTAIAFVGVNNPYQLIREKDILCTNICSQVNWLTWKANDNTLGYSYCCDVRELRGNVTNIPNFTVIEGSFP 294 HSP 5 Score: 123.635 bits (309), Expect = 4.514e-25 Identity = 70/195 (35.90%), Postives = 104/195 (53.33%), Query Frame = 0
Query: 41 YYRSQQLAQMETILGQNEDVNGNQIIESG-----FSTSLYF-ARGHLAPDADFIYDLFQDATYYFVNVAPQFQSFNNGNWKALEGAVRDYAESLKASVKVITGTYGLLRFKNTIGKLRPFFLYTEDGNQW-IPAPEYYWKVVHNEQTNEAIAFVGHNNPHSQ-QTPTPLCQSQCGKLSWADWNMDSLSSGFMYCC 227
Y R+ Q + +ILG + SG ST+ YF ARGH A ADF++ T+Y+VN APQFQ+FN GNW LE VR ++ + + + V TG +G+ + GK + +LY + N + I P YWKV+++ +T AIAFVG NNP+ + LC +++W W + + G+ Y C
Sbjct: 503 YARATQRRTINSILG----------LSSGDTTYIHSTNNYFLARGHFAAKADFVFGAQHRLTFYYVNAAPQFQTFNGGNWNTLESNVRSFSANRQLDLVVYTGGHGVTSLPDVNGKQQDLYLYKDSNNNYAISVPRLYWKVLYDPKTKTAIAFVGVNNPYQLIRRKDVLCTDISSQVNWLTWKANDTTLGYSYSC 687 HSP 6 Score: 122.479 bits (306), Expect = 1.191e-24 Identity = 65/214 (30.37%), Postives = 121/214 (56.54%), Query Frame = 0
Query: 949 LNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDY-LPVPKYFWKVVHNPQTNHAIAFVGMNDAH-LDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDLS 1160
++ Y++ +Q + + +LG L +G+ S+++ + A+GH + A FV+ Q T+Y++N APQFQ FN GNW LE +VR ++ + + + +YTG +G +N K+ + L + +Y + VP+ +WKV+++P+T AIAFVG+N+ + L +C+++ +V W ++ GY Y C V++LR + +P+ +
Sbjct: 499 VDSLYARATQRRTINSILG----LSSGDTTYIHSTNN-YFLARGHFAAKADFVFGAQHRLTFYYVNAAPQFQTFNGGNWNTLESNVRSFSANRQLDLVVYTGGHGVTSLPDVN----GKQQDLYLYKDSNNNYAISVPRLYWKVLYDPKTKTAIAFVGVNNPYQLIRRKDVLCTDISSQVNWLTWKANDTTLGYSYSCDVRELRRNITNIPNFT 703 HSP 7 Score: 122.094 bits (305), Expect = 1.659e-24 Identity = 65/213 (30.52%), Postives = 120/213 (56.34%), Query Frame = 0
Query: 311 LNEAYSKKSQVKELKMVLGGSFTLPNGEAIIDSSSSSTSYFAKGHLSPDAAFVYDVQQDATYYFMNVAPQFQAFNNGNWKALEGSVRDYAISSKGAIDIYTGTYGYLFYNKLNSDGSSKRTYIDLMRFGTKDY-LPVPKYFWKVVHNPQTNHAIAFVGMNDAH-LDFTPSRICSNVCDEVPRADWDMDNLDSGYMYCCKVKDLRDTVDYVPDL 521
++ Y++ +Q + + +LG L +G+ S+++ + A+GH + A FV+ Q T+Y++N APQFQ FN GNW LE +VR ++ + + + +YTG +G +N K+ + L + +Y + VP+ +WKV+++P+T AIAFVG+N+ + L +C+++ +V W ++ GY Y C V++LR + +P+
Sbjct: 499 VDSLYARATQRRTINSILG----LSSGDTTYIHSTNN-YFLARGHFAAKADFVFGAQHRLTFYYVNAAPQFQTFNGGNWNTLESNVRSFSANRQLDLVVYTGGHGVTSLPDVN----GKQQDLYLYKDSNNNYAISVPRLYWKVLYDPKTKTAIAFVGVNNPYQLIRRKDVLCTDISSQVNWLTWKANDTTLGYSYSCDVRELRRNITNIPNF 702 The following BLAST results are available for this feature:
BLAST of duplex-specific nuclease vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 2
BLAST of duplex-specific nuclease vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 0
BLAST of duplex-specific nuclease vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold176_size284796:44826..51368+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold176_size284796-snap-gene-0.14 ID=maker-scaffold176_size284796-snap-gene-0.14|Name=duplex-specific nuclease|organism=Tigriopus kingsejongensis|type=gene|length=6543bp|location=Sequence derived from alignment at scaffold176_size284796:44826..51368+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'duplex-specific nuclease' has the following synonyms
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