BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. L. salmonis genes
Match: EMLSAG00000005792 (supercontig:LSalAtl2s:LSalAtl2s311:368488:399678:1 gene:EMLSAG00000005792 transcript:EMLSAT00000005792 description:"maker-LSalAtl2s311-augustus-gene-3.14")

HSP 1 Score: 1200.27 bits (3104), Expect = 0.000e+0
Identity = 712/1237 (57.56%), Postives = 828/1237 (66.94%), Query Frame = 0

Query:  176 MYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGRLLPQISKSSSSQ---DGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSKSGARGR-LAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSLSDMG----------NTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNPDQGSSPSSSNGSMENLTHAEGSIQVNLLHPSQS---AAADGSRRISTCSGYSTTTD--------------------------------------NGRHGSLTPSRRISGASSLSGYYSDERERSSQDS----NHKSQHWRYSYRRYEEEQRNE--DAASSVTGTRHGMPRGSIGLLPSSRKGSHDSTASGRSGSIISNAQWRKESVTIVEQVEPIHEQDDATSDRGDLAGTRRQNSLGMAEAPSQAVAKTT------------------------SLGSDGGTEEKFLAIPKKPITASSSAETLTD-----NTLTAPPSPKSGGTPSPGRNAPGPPQRRASHVDQLVSVQSPSSMED---NEVDRPET-TPLKS----ALKKCSISSITSGPTPKSRGRSTSTFSSASYS---YQVNNDDDGSDLIAARGCSSQ-----PGSHHPSFSSPTGSRIIFGGLNLKDLTTSMKSPTKEHKKKKGIFNRHIASATS------------NDSSNDHSSSPISSPQHS-KMGVVVTSSRQAHRRSSSATAAVAFAVATAGSGNPPD---PTNK-DVFKEAAKRKMSLTSSVSTMRVLSVLRHWVTKHAQ 1289
            MYRRENQR+YELRAD+I+E                                +VESEKTAKWQYTQHC+EL +EIK +R ELF+LKKE RLLPQ+SK+SS +    G+ S +EEDPD+AKIKKVQSFFRGWLCRRRWKQIV+EYIRSPHAESMRKRNCLVFRMVEAEEEYVEQ+EILI+CF RPFKMAASSKNPPC H+DINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLG    DLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQ P F  LL+RLEKKPACKGRSLE FLTYPMHQIPRYIITLH+LLAHTPPDHVEKKSLEHAR QLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQ+FVRQGTL+QILP+ S S      L IGREKEREAVRQCFLFSNHLI+TTRTQAGRLHLV+G GKIPLA+VTL+EDP+E++R D+ E P  S    SLSD G          N   +++  DFK+I                   QEKA+WITDI+QCMD VHFNGLFYNT+PNASSST+PQCVKSDP+LFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFT+ ITVLEALK VF   +   S SSS  S+ENL   EG  Q ++ H   S      +G RRISTCSGYST T                                       N RHGSLTP RRISGASS+SGY SDER   S +     + K QHWRYS+RRYEEEQR      +S++         GS       RKGS+DS  SGRSGSI+S   +RKESV I+EQ EPI E DDA+S+ G     + +  + ++ +    V+K T                         + SD GT   +LA+P   + ASSSAETLTD     N        +S    S   +   P +R+ S        + PS + D     V+ P+    LK     A+++  +  +    T K+  R+ ++ SS+      Y  N ++   D   A    S      PGSHH SFSS + +R +FGG+  KD     KSPT+E +KKKG+F+    ++ S            N  SN  +++PISSPQHS K+GV++TSSRQA+RRSSSA A++AFAVATAGSGNP +   P  K + FKE +KRK S++S+ +TMRVLSVLRHWVTKH Q
Sbjct:    1 MYRRENQRQYELRADSINE--------------------------------LVESEKTAKWQYTQHCEELTEEIKKLRNELFSLKKEWRLLPQMSKTSSQEGRSSGNSSVEEEDPDLAKIKKVQSFFRGWLCRRRWKQIVDEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQMEILITCFYRPFKMAASSKNPPCTHDDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLG----DLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQSPSFAQLLNRLEKKPACKGRSLEIFLTYPMHQIPRYIITLHDLLAHTPPDHVEKKSLEHARDQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQVFVRQGTLIQILPSPSSSRPGRSRLNIGREKEREAVRQCFLFSNHLIMTTRTQAGRLHLVEGTGKIPLANVTLIEDPNEDDRNDDQEYPGSSG---SLSDEGEGNTPNTGGKNNLNQNEKKDFKII-------------------QEKAAWITDISQCMDNVHFNGLFYNTLPNASSSTLPQCVKSDPSLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTDSITVLEALKNVFNTSEPLESSSSSLSSIENL--GEGHTQ-HVHHQVTSIPSTECEGKRRISTCSGYSTATGIRNNLVCVFHPKYHLESNDSDNNLANLPNVSHHPDTGTNSRHGSLTP-RRISGASSVSGYCSDERGSFSTEGGLTPHMKPQHWRYSFRRYEEEQRETTLGVSSNLQNVEVLSRFGSGSGTGGRRKGSYDSICSGRSGSIVST--FRKESVPIIEQTEPISEMDDASSN-GTSTTEKMEEEISISHS---NVSKDTQXSSSSXSSSXATSSINTDNKSLVKVKSDTGT--PYLAVPPH-LAASSSAETLTDDPKEKNVQKEVEEGQSSAKVSEKHHGXQPMKRQNS-------FEEPSKIADLAKTMVNMPKADITLKGIADKAVEEVGLVGLAHKFTRKTGIRTNTSSSSSGSYTTPYTFNLEEGEKDATVASSGGSTGGTSIPGSHHSSFSSTSTTRALFGGITFKDF----KSPTREIRKKKGLFHNEFCNSGSSSISGPSGGTSQNPGSN--TNTPISSPQHSSKIGVIITSSRQANRRSSSACASIAFAVATAGSGNPSNNRSPAKKSEDFKELSKRKGSMSSNATTMRVLSVLRHWVTKHPQ 1153          

HSP 2 Score: 426.787 bits (1096), Expect = 4.306e-124
Identity = 198/256 (77.34%), Postives = 226/256 (88.28%), Query Frame = 0

Query: 1561 QVPSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSSFGHSV 1816
            + PSKE I SL ALEIAEQM +LDQKILF I  SEFLGQAWTK D+++R+P+IV MTK FN+ SRLV S+I++Q  +  R+ VIEKWTAVADICRCLHNFNGVLQICAAFTNS+VFRLKNTWNRVSKS + TI+KLQ+LVSSDGRFRNLRD LHRTDPPCIPYLGMYLSDLTFIEEGTPNFT++ LLNFAKMRMIAHVIREIRQFQQTPYKIE++PKV NY+LD  + +DDE +YQ+SL+VEP  SRLSSSFG S+
Sbjct: 1213 ETPSKETILSLLALEIAEQMTYLDQKILFAIPCSEFLGQAWTKKDRNIRAPNIVQMTKFFNDFSRLVCSEIISQTCLNSRIMVIEKWTAVADICRCLHNFNGVLQICAAFTNSSVFRLKNTWNRVSKSVRHTIDKLQSLVSSDGRFRNLRDTLHRTDPPCIPYLGMYLSDLTFIEEGTPNFTESGLLNFAKMRMIAHVIREIRQFQQTPYKIERIPKVVNYLLDSGKLMDDEEMYQRSLMVEPXASRLSSSFGXSM 1468          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. L. salmonis genes
Match: EMLSAG00000007337 (supercontig:LSalAtl2s:LSalAtl2s414:278264:289131:1 gene:EMLSAG00000007337 transcript:EMLSAT00000007337 description:"augustus_masked-LSalAtl2s414-processed-gene-2.1")

HSP 1 Score: 135.576 bits (340), Expect = 5.962e-32
Identity = 73/223 (32.74%), Postives = 134/223 (60.09%), Query Frame = 0

Query: 1591 IQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNH-LLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYI--LDP-----ERQLDDEMLYQQSLIVEPRT 1805
            ++ SE + QAWT+ DKD RSP+++ M +     +R +   IV   ++  R  ++++   +  + +  +NFNGVL I +A  +SAV RL+ T ++V+    +++E+  + +  +  F+   + L   +PPC+P+ G Y +++ F+EEG  +F   H L+NF+K R +A +I EI+Q+Q  PY + +V K+ +Y+  L+P     E+++ D  L+Q S+ +EPR 
Sbjct:  759 VKPSELVDQAWTREDKDKRSPNLLRMVRHTTNFTRTLEKLIVETENIEERGCLVQRILEIMLVLQEHNNFNGVLAITSALNSSAVHRLQLTKDKVANHLLKSLEEASSYI--EDHFKIYWEKLRSINPPCVPFFGQYQTNILFLEEGNLDFLPPHSLINFSKRRKVAEIISEIQQYQNQPYCLSEVKKLKDYLENLEPFPELCEKEITDH-LWQHSITIEPRV 978          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. L. salmonis genes
Match: EMLSAG00000008301 (supercontig:LSalAtl2s:LSalAtl2s502:91443:143170:-1 gene:EMLSAG00000008301 transcript:EMLSAT00000008301 description:"maker-LSalAtl2s502-augustus-gene-1.9")

HSP 1 Score: 106.686 bits (265), Expect = 2.737e-23
Identity = 65/240 (27.08%), Postives = 124/240 (51.67%), Query Frame = 0

Query: 1567 NINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTS 1806
            NI +  + ++AEQM +LD ++   + + E +   W +   + +S ++   T+ FN MS    + I+ Q D   R + + K+  +    R  +NFN  L I +A  ++ + RL+  W+   K+    +++  +L+ S   FR  R AL+ +  PCIPY+G+ L DLTF+  G  +      +NFAK     +++  +R+F++  Y  ++   + ++       L ++ L+Q S  ++PR S
Sbjct:  644 NILNFKSTDLAEQMTYLDYQLFIRLDTVELI--LWVQEQNEDKSYNLTKFTEHFNNMSFWCRTQILQQDDSKEREKYMTKFIKIMKFLRKYNNFNSYLAILSALDSAPIRRLE--WH---KNIIDNLKEYCSLIDSSSSFRAYRQALNESGSPCIPYVGLILQDLTFVHIGNSDIYHGK-INFAKRWQQFNILDNLRRFRKENYHFKRREDIISFFDGFSLYLSEDELWQLSESIKPRCS 875          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. L. salmonis genes
Match: EMLSAG00000011695 (supercontig:LSalAtl2s:LSalAtl2s812:185767:188618:-1 gene:EMLSAG00000011695 transcript:EMLSAT00000011695 description:"maker-LSalAtl2s812-snap-gene-1.92")

HSP 1 Score: 103.219 bits (256), Expect = 3.999e-23
Identity = 66/239 (27.62%), Postives = 120/239 (50.21%), Query Frame = 0

Query: 1576 IAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRD-----ALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYK-IEQVPKVTNYI-----LDPERQLDDEMLYQQSLIVEP 1803
            +A Q+  LD  +   I   E    +WTK +K   +P++V  T RFN +S   V +I+       R +V+  +  +A     L+N +    + +A  ++ ++RL  TWN +++  K + +KL  L S    F  LRD     AL   D  CIPYLG+YL+DL +++   P+     +    +   + +++R + + QQ+ Y  ++ +P V  Y+     +D  ++  ++  Y+ SL +EP
Sbjct:   44 LASQLTLLDAPVFNAIAPEELSSCSWTKKNKHSVAPNVVAFTLRFNHVSFWTVQEILRYETPKTRAEVLSHFIRLAKKLHELNNLHSEFAVLSALQSAPIYRLNKTWNCLTRRDKTSFDKLVDLFSERDNFSKLRDHMNTTALRHRD--CIPYLGLYLTDLVYVDMAHPH--SGGIEPPQRQLKMNNILRIVSELQQSSYSHLQCIPXVQQYLKSIRYIDELQKFIEDDHYKLSLKLEP 278          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. L. salmonis genes
Match: EMLSAG00000003617 (supercontig:LSalAtl2s:LSalAtl2s1983:17358:19185:-1 gene:EMLSAG00000003617 transcript:EMLSAT00000003617 description:"maker-LSalAtl2s1983-augustus-gene-0.7")

HSP 1 Score: 92.4337 bits (228), Expect = 3.676e-19
Identity = 60/241 (24.90%), Postives = 126/241 (52.28%), Query Frame = 0

Query:  327 RNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTEC-KQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSKSGAR 566
            R+  +  +VE+E+ Y++ LEIL + F  P K    SK P   + D  S+F +  T+  ++   L+ + +  +          +G +F  L P L  Y  Y       ++++ +  ++  +FG +L+  E +P   G  L + L  P+ +IPRY + L +LL +TP  H ++  L  A ++++ ++R +  ++ E EN R+ L I++ +++    ++   ++ ++ G L+++  +GS +G +
Sbjct:   47 RSKAIKELVESEKSYLKHLEILENFFSEPLK----SKFP--KYSDFKSVFGDIPTIRKVNAELLEAIESSKDR---------IGLVFLELAPYLKFYSTYANEFASNVKLVEKLTEENKEFGRVLNSRESRPEALGLKLNSLLITPIQRIPRYKLLLEDLLKNTPTQHPDRLDLTKALLEIDSVARYIDTQIGEHENARRTLDIQKSLVKNLPKIVKPGRILLKTGLLMKVPRSGSGNGVQ 272          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. L. salmonis genes
Match: EMLSAG00000009657 (supercontig:LSalAtl2s:LSalAtl2s620:174729:288246:1 gene:EMLSAG00000009657 transcript:EMLSAT00000009657 description:"maker-LSalAtl2s620-augustus-gene-1.20")

HSP 1 Score: 90.1225 bits (222), Expect = 4.189e-18
Identity = 65/259 (25.10%), Postives = 120/259 (46.33%), Query Frame = 0

Query: 1518 KSGMTQNWFFKSTDEGEEPEESKGKKSGTLKPSASSGALNGLRQVPSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQP-DVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSD---GRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKI 1772
            + G+   ++ KS    E           TL P  ++  L    Q  S   +  L+  E+A Q+   D  I   I+ +E++   + K  +   +P++   ++  N      +++IV +  ++  R+++I+++  +A  C+   NFN +  I +   +++V RLK TW +V     +   ++Q L+       R+RNL +      PP IP+  +   DLTFI     +  DN L+NF K+RMIA  +R +      P  +
Sbjct: 1042 RMGLASRYYIKSVGCSE-----------TLLPDDAAAEL----QKDSSVXLLXLNXXEVATQLMVEDFTIFRQIEGTEYVTDLFGKKSR-YGNPNLTRFSQLVNREMMWAITEIVMESSNLNKRMRIIKQFIKIARQCKETQNFNSMFAIISGLGHASVSRLKATWEKVPAKYLRIFHEMQQLMDPSRNMSRYRNLVNG-EDVQPPIIPFYPIVKKDLTFIHIANDSVIDN-LVNFEKLRMIAKEVRSLTNMCSAPLDL 1282          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. L. salmonis genes
Match: EMLSAG00000008585 (supercontig:LSalAtl2s:LSalAtl2s52:300085:322987:1 gene:EMLSAG00000008585 transcript:EMLSAT00000008585 description:"maker-LSalAtl2s52-augustus-gene-3.15")

HSP 1 Score: 80.4925 bits (197), Expect = 2.732e-15
Identity = 58/211 (27.49%), Postives = 95/211 (45.02%), Query Frame = 0

Query: 1592 QSSEFLGQAWTKPDK--DVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGI---RVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHR-----------TDPP-------------------CIPYLGMYLSDLTFIEEGTPNFT-DNHLLNFAKMRMIAHVIREIRQFQ 1766
            Q +  LG  W++ DK  D  +  ++   ++FN +S  V+S ++  P  G    R +VI KW  +A   R L NF+ +  I +   ++ ++RLK +W+ VS+   +  E+L  + S D      R+ L R            + P                    IPYLG +L+DLT I+   P+      L+NF K R    V+ +I+  Q
Sbjct:  241 QMNSCLGSVWSRRDKSRDRDAATVLATVEQFNAVSFRVISTVLIGPVDGKASSRAKVISKWIDIAQELRVLKNFSSLKAIISGLQSNPIYRLKKSWSYVSRDKIEVYEELARIFSEDNNALAQRELLVREGTARFAESSGNNEPHLQKVLLKHSENSRALSYGTIPYLGTFLTDLTMIDTAIPDIVPPEGLINFDKKRKEFEVLAQIKLLQ 451          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. L. salmonis genes
Match: EMLSAG00000008338 (supercontig:LSalAtl2s:LSalAtl2s509:237196:263796:-1 gene:EMLSAG00000008338 transcript:EMLSAT00000008338 description:"maker-LSalAtl2s509-snap-gene-2.19")

HSP 1 Score: 70.8626 bits (172), Expect = 3.018e-12
Identity = 73/344 (21.22%), Postives = 155/344 (45.06%), Query Frame = 0

Query:  326 KRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLP---MLSIYQEYVRNHHYCLQVLT-ECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDV-NQMFVRQGTLVQILPAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGK-----------IPLADVTLVEDP---SEEERMDEPESPSGSSDSSSLSDMGNTRY 650
            KR  ++  +V +E  YV  L+ L++ + +P +    S     +   I ++F     +L +H +F  GL+  + SW +      +GD+F        +L  Y +++ N +    ++  E K+       L  L++  +    SL   L  P+ + P++I+ L +LL  TPP HV++ +L+ A   LE L+  +++   E E         + I      LL+  ++  +++G L Q+     +  + G+++      R   R+  L ++++I    + +GR   +D +G+           +P+ D+ +++     +   R+  P +   S    ++S+  +T Y
Sbjct:  105 KRRYIIQTLVSSENNYVSSLQRLVNDYKKPLE---ESSPLIISQSKITTLFYRISEILQIHYLFRIGLSQMILSWDS---QEKIGDVFVASFSKAIVLETYSDFINNFNEAFNIVKYESKRKSALSDFLD-LKQLTSSDRISLFGLLVKPVQRFPQFILILQDLLKETPPGHVDRMALQLALTTLESLAEMLNERKREAEQFAAFKEKIKSIGGKTKGLLECKDRYLLKEGDLHQL-----EFNSSGQIS------RSKARRLLLINDYIICL--SMSGRFSEID-LGQPSPRRYSLKWAVPVIDIEIIDGKPGGTTLSRLLAPSTSGLSYKKPTVSNKNSTSY 427          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. L. salmonis genes
Match: EMLSAG00000002673 (supercontig:LSalAtl2s:LSalAtl2s154:239531:245063:1 gene:EMLSAG00000002673 transcript:EMLSAT00000002673 description:"maker-LSalAtl2s154-snap-gene-2.5")

HSP 1 Score: 70.0922 bits (170), Expect = 4.687e-12
Identity = 51/225 (22.67%), Postives = 103/225 (45.78%), Query Frame = 0

Query:  334 MVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLH-QIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTEC-KQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQI 556
            ++  EE+YV  L ++   F         ++N   + + +  +F N +++   H +  L  L  RME+W        +GD+F    P   +Y EYV+N    + ++     +  +F  ++  +   P C   SL+  +  P+ +IPRY + L + L   P D  +++  E A   +   +   +D +   E  +K L ++  +    D L++  +  V++G +V+I
Sbjct:   99 LLSTEEQYVRVLYLIDQVFHTRVDQENRTQNL-FSADVLPQMFANIKSIYKFHAEFLLPQLRERMENWKGS--NQRIGDIFVKFSPFFKMYTEYVKNFGSAIHLINHIYNKNSKFAGIMDDIHSMPECGNLSLQHHMLTPIQRIPRYEMLLKDYLKKLPEDSDDREDSEKALHLVSTAAAHANDAMKRIEKFKKLLEVQECLSGTVD-LVNPTRELVKEGKIVKI 319          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. L. salmonis genes
Match: EMLSAG00000007768 (supercontig:LSalAtl2s:LSalAtl2s453:563174:567050:-1 gene:EMLSAG00000007768 transcript:EMLSAT00000007768 description:"maker-LSalAtl2s453-snap-gene-4.55")

HSP 1 Score: 63.5438 bits (153), Expect = 4.805e-10
Identity = 54/227 (23.79%), Postives = 106/227 (46.70%), Query Frame = 0

Query:  334 MVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTEC-KQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTP---PDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQI 556
            ++ +EE YV  L++L   F            PPC   ++N I      +  L+   L   T+R+ +W +      + D+  +    L IY  Y++      Q   EC K+ P+F  L+   + K  C+   +E  +  P+ ++PRY +     + + P   PD+  + SLE   I L+ ++ + + E+ E +  +K L ++  + E  D++    ++F ++G L++I
Sbjct:  260 IMTSEEAYVNNLKLLNVDFRAFVSKEIDDAIPPC---ELNKILSYFPELQLLNSDLLNDFTSRIRNWDS---NPKIADIMVIKGKFLLIYASYIKEFPRNSQFYQECLKKYPKFRELVLAFQLKKECRNLKIEHLMLEPIQRLPRYKLLFERYVKNLPVGDPDY--EVSLEALEIILK-IADKANTELKEYDEFKKMLNLQSRVNES-DLIQPDRELF-KEGELLKI 475          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. SwissProt
Match: gi|1708003|sp|P27671.2|RGRF1_MOUSE (RecName: Full=Ras-specific guanine nucleotide-releasing factor 1; Short=Ras-GRF1; AltName: Full=CDC25Mm; AltName: Full=Guanine nucleotide-releasing protein; Short=GNRP; AltName: Full=Ras-specific nucleotide exchange factor CDC25)

HSP 1 Score: 672.544 bits (1734), Expect = 0.000e+0
Identity = 343/641 (53.51%), Postives = 445/641 (69.42%), Query Frame = 0

Query:  158 SCEESGPDQISSSYCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFA---LKKEGRLLPQISKSSSSQDGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNP 795
            S +ESG  Q    + F + +  ++Q+  ELR +   +C  W+  I  AS+  L  + E L QK+LHLLQVVE+EKT   Q  Q  ++ + EI+ ++ E+     +K   R+       SS++ G  S D+ED DI KIKKVQSF RGWLCRR+WK I+++YIRSPHA+SMRKRN +VF M+EAE EYV+QL IL++ FLRP +MAASSK PP  H+D++SIFLNSET++FLHQIF +GL AR+ SWPTLVL     DLFD+LLPML+IYQE+VRNH Y LQ+L  CKQ   F  LL + E KP C+ R+LETFLTYPM QIPRYI+TLHELLAHTP +HVE+ SL++A+ +LE+LSR MHDEVSETENIRKNLAIERMI EGC+ILLD +Q FVRQG+L+Q+  +     +RGRL      ++E  RQCFLFS HLI+ TR   G+LHL    G I L D TL+++P   E +D+    +G               E + ++FK+ ++  K     T+ L+A T QEKA+W +DI QC+D +  NGL  N     S  T+PQ +KSD +L+ DDVDIRFSKT N+CK+ QIRYA+ ERLL+RLTDLRFL+IDFLNTFL +YRVFTN + VL+ L  ++  P
Sbjct:   84 SSKESGEKQ---QHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEVTITNLIKDNDRI------QSSNKAG--SADDEDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLA----DLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQMSLSEKSKSSRGRLG-SLSTKKEGERQCFLFSKHLIICTRGSGGKLHLTKN-GVISLIDCTLLDEP---ENLDDEAKGAGP--------------EIEHLEFKIGVEP-KDSLPFTVILVASTRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTNAMVVLDKLINIYRKP 689          

HSP 2 Score: 303.138 bits (775), Expect = 8.902e-83
Identity = 134/232 (57.76%), Postives = 174/232 (75.00%), Query Frame = 0

Query: 1573 ALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPR 1804
            ALEIAEQ+  LD  +  +I   EF GQ W K +K  R+P+I+  TK FN +S  + S+I+   D+  R   IEKW AVADICRCLHN+N VL+I ++   SA+FRLK TW +VSK +K  ++KLQ LVSSDGRF+NLR++L   DPPC+PYLGMYL+DL FIEEGTPN+T++ L+NF+KMRMI+H+IREIRQFQQT YKI+  PKV  Y+LD    LD+E LY+ SL++EP+
Sbjct: 1028 ALEIAEQLTLLDHLVFKSIPYEEFFGQGWMKAEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDISARASAIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLRESLRNCDPPCVPYLGMYLTDLVFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIDPQPKVIQYLLDESFMLDEESLYESSLLIEPK 1259          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. SwissProt
Match: gi|162416039|sp|A2CEA7.1|RGRF2_DANRE (RecName: Full=Ras-specific guanine nucleotide-releasing factor 2; Short=Ras-GRF2; AltName: Full=Ras guanine nucleotide exchange factor 2)

HSP 1 Score: 672.159 bits (1733), Expect = 0.000e+0
Identity = 338/637 (53.06%), Postives = 442/637 (69.39%), Query Frame = 0

Query:  163 GPDQISSSYCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKK-EGRLLPQISKSSSSQDGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQI--LPAGSKSGAR-GRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNP 795
            G D +     F +++  + Q+  ELR +  S+C  W++ I+ AS++ + ++ E L QK++HL+Q+VE+EK A  Q     ++   EI+ ++ E+ AL K + R+ P              ++EEDPDI KIKKVQSF RGWLCRR+WK IV++YI SPHAESMRKRN +VF MVEAE EYV QL IL++CFLRP +MAASSK PP +H+D++SIFLNSET++FLH+IF +GL AR+ +WPTLVL     DLFD+LLPML+IYQE+VRNH Y LQVL  CKQ   F  LL + E   AC+GR LETFLTYPM QIPRYIITLHELLAHTP +HVE+KSLE A+ +LE+LSR MHDEVS+TENIRKNLAIERMI+EGCDILLD +Q FVRQG+L+Q+  +  G  S  R G L++ +E E    RQCFLF+ H ++ TR+  G+LHL+   G + L + TL+E+P      DE    SG              + H  +DFK++++        T+ L+AP+ QEKA+W +DI+QC+D +  NGL  +     S  T+P  +KSD  L KDDVDI FSKT N+CK+PQIRYA+ ERLL+RLTDLRFL+IDFLNTFL TYR+FT    V+E L  ++  P
Sbjct:   85 GKDALDKLQYFLVVFGHDGQKPLELRTEEESDCDEWVEAIQQASYSDIIIEREVLMQKYIHLVQIVETEKVAANQLRTQLEDQDTEIERLKAEIIALNKTKERMRPY----------HVFQEEEDPDIKKIKKVQSFMRGWLCRRKWKIIVQDYICSPHAESMRKRNQIVFNMVEAETEYVHQLSILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLVLA----DLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYESNAACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSVERGKLSKVRLGSLSLKKEGE----RQCFLFTKHFLICTRSSGGKLHLLKQGGVLSLIECTLIEEPDAN---DEDAKNSGQV------------FGH--LDFKIVVEP-SDAPAFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMIKSDARLHKDDVDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVMEKLADIYKKP 685          

HSP 2 Score: 308.531 bits (789), Expect = 1.383e-84
Identity = 141/246 (57.32%), Postives = 182/246 (73.98%), Query Frame = 0

Query: 1559 LRQVPSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPR 1804
            + + P  E   SLSA+EIAEQ+  LD  +  +I   EFLGQ W K DK  R+P+I+  ++ FN+MS LV S I++  DVG R   IEKW AVADICRCL+N+NGVL+I +A   SA++RLK TW +V K +K  ++KLQ  VSS+GRF+NLR+ L   +PPC+PYLGMYL+DL FIEEGTPNFT+  L+NF+KMRMI+H+IREIRQFQQTPY+IE  PKVT Y+LD    +D++ LY  SL +EPR
Sbjct:  996 MAECPKAECFESLSAMEIAEQITLLDHIVFRSIPYEEFLGQGWMKTDKTERTPYIMKTSQHFNDMSNLVASQIMSHTDVGSRAGSIEKWVAVADICRCLNNYNGVLEITSALNRSAIYRLKKTWAKVCKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPCVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTPYRIEHQPKVTQYLLDKTLIMDEDTLYDLSLKIEPR 1241          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. SwissProt
Match: gi|162416200|sp|O14827.2|RGRF2_HUMAN (RecName: Full=Ras-specific guanine nucleotide-releasing factor 2; Short=Ras-GRF2; AltName: Full=Ras guanine nucleotide exchange factor 2)

HSP 1 Score: 663.3 bits (1710), Expect = 0.000e+0
Identity = 335/635 (52.76%), Postives = 440/635 (69.29%), Query Frame = 0

Query:  165 DQISSSYCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKK-EGRLLPQISKSSSSQDGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQI--LPAGSKSGAR-GRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNP 795
            D +   Y F +++  E Q+  ELR +   + + W++ I  AS+  + ++ E L QK++HL+Q+VE+EK A  Q     ++   EI+ ++ E+ AL K + R+ P  S          ++++EDPDI KIKKVQSF RGWLCRR+WK IV++YI SPHAESMRKRN +VF MVEAE EYV QL IL++ FLRP +MAASSK PP +H+D++SIFLNSET++FLH+IF +GL AR+ +WPTL+L     DLFD+LLPML+IYQE+VRNH Y LQVL  CKQ   F  LL + E  PAC+GR LETFLTYPM QIPRYIITLHELLAHTP +HVE+KSLE A+ +LE+LSR MHDEVS+TENIRKNLAIERMI+EGCDILLD +Q F+RQG+L+Q+  +  G  S  R G L++ +E E    RQCFLF+ H ++ TR+  G+LHL+   G + L D TL+E+            P  S D S  S      + H  +DFK++++   +    T+ L+AP+ QEKA+W++DI+QC+D +  NGL        S  T+P  +KSD  L KDD DI FSKT N+CK+PQIRYA+ ERLL+RLTDLRFL+IDFLNTFL TYR+FT    VL  L  ++  P
Sbjct:   91 DALDKQYYFTVLFGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQS----------NQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILA----DLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGE----RQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIDCTLIEE------------PDASDDDSKGS---GQVFGH--LDFKIVVEPPDAA-AFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRP 689          

HSP 2 Score: 299.671 bits (766), Expect = 9.757e-82
Identity = 137/239 (57.32%), Postives = 178/239 (74.48%), Query Frame = 0

Query: 1566 ENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPR 1804
            E   SLSA+E+AEQ+  LD  I  +I   EFLGQ W K DK+ R+P+I+  ++ FN+MS LV S I+   DV  R   IEKW AVADICRCLHN+NGVL+I +A   SA++RLK TW +VSK +K  ++KLQ  VSS+GRF+NLR+ L   +PP +PYLGMYL+DL FIEEGTPNFT+  L+NF+KMRMI+H+IREIRQFQQT Y+I+  PKV  Y+LD +  +D++ LY+ SL +EPR
Sbjct:  996 ECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVAQYLLDKDLIIDEDTLYELSLKIEPR 1234          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. SwissProt
Match: gi|81908500|sp|P70392.2|RGRF2_MOUSE (RecName: Full=Ras-specific guanine nucleotide-releasing factor 2; Short=Ras-GRF2; AltName: Full=Ras guanine nucleotide exchange factor 2)

HSP 1 Score: 662.529 bits (1708), Expect = 0.000e+0
Identity = 336/642 (52.34%), Postives = 440/642 (68.54%), Query Frame = 0

Query:  162 SGP----DQISSSYCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKK-EGRLLPQISKSSSSQDGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQI--LPAGSKSGAR-GRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNP 795
            +GP    D +   Y F +++  + Q+  ELR +     + W++ I  AS+  + ++ E L QK++HL+Q+VE+EK A  Q     ++   EI+ ++ E+ AL K + R+ P              ++EEDPDI KIKKVQSF RGWLCRR+WK IV++YI SPHAESMRKRN +VF MVEAE EYV QL IL++ FLRP +MAASSK PP NH+D++SIFLNSET++FLH+IF +GL AR+ +WPTLVL     DLFD+LLPML+IYQE+VRNH Y LQVL  CKQ   F  LL + E  PAC+GR LETFLTYPM QIPRYIITLHELLAHTP +HVE+KSLE A+ +LE+LSR MHDEVS+TENIRKNLAIERMI+EGCDILLD +Q F+RQG+L+Q+  +  G  S  R G L++ +E E    RQCFLF+ H ++ TR+  G+LHL+   G + L   TL+E+            P GS D     D   + +    +DFK++++   + +  T+ L+AP+ QEKA+W++DI+QC+D +  NGL        S  T+P  +KSD  L KDD DI FSKT N+CK+PQIRYA+ ERLL+RLTDLRFL+IDFLNTFL TYR+FT    VL  L  ++  P
Sbjct:   84 AGPAGARDALDKQYYFTVLFGHDGQKPLELRCEEEQAGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIATNQLRHQLEDQDTEIERLKSEIVALNKTKERMRPY----------HVHQEEEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAETEYVHQLYILVNGFLRPLRMAASSKKPPINHDDVSSIFLNSETIMFLHEIFHQGLKARLANWPTLVLA----DLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGE----RQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIQCTLIEE------------PDGSDD-----DPKGSGHMFGHLDFKIVVEPPDAAS-FTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTATVVLAKLSDIYKRP 689          

HSP 2 Score: 301.597 bits (771), Expect = 1.115e-82
Identity = 137/242 (56.61%), Postives = 179/242 (73.97%), Query Frame = 0

Query: 1563 PSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPR 1804
            P  E   +LSA+E+AEQ+  LD  +  +I   EFLGQ W K DK+ R+P+I+  ++ FNEMS LV S I+   D+  R   IEKW AVADICRCLHN+NGVL+I +A   SA++RLK TW +VSK +K  ++KLQ  VSS+GRF+NLR+ L   +PP +PYLGMYL+DL FIEEGTPNFT+  L+NF+KMRMI+H+IREIRQFQQT Y+I+Q PKV  Y+LD    +D++ LY+ SL +EPR
Sbjct:  945 PKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNEMSNLVASQIMNYADISSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTAYRIDQQPKVIQYLLDKALVIDEDSLYELSLKIEPR 1186          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. SwissProt
Match: gi|121515|sp|P28818.1|RGRF1_RAT (RecName: Full=Ras-specific guanine nucleotide-releasing factor 1; Short=Ras-GRF1; AltName: Full=Guanine nucleotide-releasing protein; Short=GNRP; AltName: Full=P140 Ras-GRF)

HSP 1 Score: 662.144 bits (1707), Expect = 0.000e+0
Identity = 338/627 (53.91%), Postives = 436/627 (69.54%), Query Frame = 0

Query:  173 FAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGRLLPQISKSSSSQDGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSK----SGARGRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNP 795
            F + +  ++Q+  ELR D   +C  W+  I  AS+  L  + E L QK+LHLLQVVE+EKT   Q  Q  ++ + EI+ ++ E+  L K+   + Q ++  + +D       ED DI KIKKVQSF RGWLCRR+WK I+++YIRSPHA+SMRKRN +VF M+EAE EYV+QL IL++ FLRP +MAASSK PP  H+D++SIFLNSET++FLHQIF +GL AR+ SWPTLVL     DLFD+LLPML+IYQE+VRNH Y LQ+L  CKQ   F  LL + E KP C+ R+LETFLTYPM QIPRYI+TLHELLAHTP +HVE+ SL++A+ +LE+LSR MHDEVSETENIRKNLAIERMI EGC+ILLD +Q FVRQG+L+Q+ P   K     G  G L++ +E E    RQCFLFS HLI+ TR    +LHL    G I L D TL++DP   E MD+              D      +H  +DFK+ ++  K     T+ L+A + QEKA+W +DI QC+D +  NGL  N     S  T+PQ +KSD +L+ DDVDIRFSKT N+CK+ QIRYA+ ERLL+RLTDLRFL+IDFLNTFL +YRVFT+ + VL+ L +++  P
Sbjct:   95 FTVNFSNDSQKSLELRTDDSKDCDEWVAAIARASYKILATEHEALMQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKAEIANLIKDNERI-QSNQLVAPED-------EDSDIKKIKKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARIASWPTLVLA----DLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRVMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLIQV-PMSEKGKINKGRLGSLSLKKEGE----RQCFLFSKHLIICTRGSGSKLHLTKN-GVISLIDCTLLDDP---ENMDD--------------DGKGQEVDH--LDFKIWVEP-KDSPPFTVILVASSRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTDAVVVLDKLISIYKKP 683          

HSP 2 Score: 302.753 bits (774), Expect = 9.970e-83
Identity = 136/233 (58.37%), Postives = 175/233 (75.11%), Query Frame = 0

Query: 1572 SALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPR 1804
            SA+EIAEQ+  LD  +  +I   EF GQ W K DK+ R+P+I+  T+ FN +S L+ S+I+   +V  R   IEKW AVADICRCLHN+N VL+I ++   SA+FRLK TW +VSK +K   +KLQ LVSSDGRF+NLR+ L   DPPC+PYLGMYL+DL F+EEGTPN+T++ L+NF+KMRMI+H+IREIRQFQQT YKIE  PKVT Y++D    LDDE LY+ SL +EP+
Sbjct: 1009 SAMEIAEQLTLLDHLVFKSIPYEEFFGQGWMKADKNERTPYIMKTTRHFNHISNLIASEILRNEEVSARASTIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWLKVSKQTKSLFDKLQKLVSSDGRFKNLRETLRNCDPPCVPYLGMYLTDLAFLEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIEPQPKVTQYLVDETFVLDDESLYEASLRIEPK 1241          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. SwissProt
Match: gi|81916556|sp|Q99JE4.1|RGRF2_RAT (RecName: Full=Ras-specific guanine nucleotide-releasing factor 2; Short=Ras-GRF2; AltName: Full=Ras guanine nucleotide exchange factor 2)

HSP 1 Score: 658.292 bits (1697), Expect = 0.000e+0
Identity = 334/642 (52.02%), Postives = 439/642 (68.38%), Query Frame = 0

Query:  162 SGP----DQISSSYCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKK-EGRLLPQISKSSSSQDGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQI--LPAGSKSGAR-GRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNP 795
            +GP    D +   Y F +++  E Q+  ELR +     + W++ I  AS+  + ++ E L QK++HL+Q+VE+EK A  Q     ++   EI+ ++ E+ AL K + R+ P              ++EEDPDI KIKKVQSF RGWLCRR+WK IV++YI SPHAESMRKRN +VF MVEAE EYV QL IL++ FLRP +MAASSK PP +H+D++SIFLNSET++FLH+IF +GL AR+ +WPTLVL     DLFD+LLPML+IYQE+VRNH Y LQVL  CKQ   F  LL + E  PAC+GR LETFLTYPM QIPRYIITLHELLAHTP +HVE+KSLE A+ +LE+LSR MHDEVS+TENIRKNLAIERMI+EGCDILLD +Q F+RQG+L+Q+  +  G  S  R G L++ +E E    RQCFLF+ H ++ TR+  G+LHL+   G + L   TL+E+P   +  D+ + P                +    +DFK++++   +    T+ L+AP+ QEKA+W++DI+QC+D +  NGL        S  T+P  +KSD  L KDD DI FSKT N+CK+PQIRYA+ ERLL+RLTDLRFL+IDFLNTFL TYR+FT    VL  L  ++  P
Sbjct:   84 AGPAGARDALDKQYYFTVLFGHEGQKPLELRCEEEQAGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIVALNKTKERMRPY----------HTHQEEEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARLANWPTLVLA----DLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEGNPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGE----RQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIQCTLIEEPDTSD--DDTKGPG---------------HMFGHLDFKIVVEPPDAA-PFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRP 689          

HSP 2 Score: 302.753 bits (774), Expect = 6.216e-83
Identity = 137/242 (56.61%), Postives = 179/242 (73.97%), Query Frame = 0

Query: 1563 PSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPR 1804
            P  E   +LSA+E+AEQ+  LD  +  +I   EFLGQ W K DK+ R+P+I+  ++ FNEMS LV S I+   D+  R   IEKW AVADICRCLHN+NGVL+I +A   SA++RLK TW +VSK +K  ++KLQ  VSS+GRF+NLR+ L   +PP +PYLGMYL+DL FIEEGTPNFT+  L+NF+KMRMI+H+IREIRQFQQT Y+I+Q PKV  Y+LD    +D++ LY+ SL +EPR
Sbjct:  946 PKAECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNEMSNLVASQIMNYADISSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWTKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTAYRIDQQPKVIQYLLDKALVIDEDTLYELSLKIEPR 1187          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. SwissProt
Match: gi|613504205|sp|Q13972.2|RGRF1_HUMAN (RecName: Full=Ras-specific guanine nucleotide-releasing factor 1; Short=Ras-GRF1; AltName: Full=Guanine nucleotide-releasing protein; Short=GNRP; AltName: Full=Ras-specific nucleotide exchange factor CDC25)

HSP 1 Score: 655.596 bits (1690), Expect = 0.000e+0
Identity = 343/648 (52.93%), Postives = 442/648 (68.21%), Query Frame = 0

Query:  162 SGPDQISSSYCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGRLLPQISKSSSSQDGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSKSGARGRL-AIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVK-------------SDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNP 795
            S  + +   + F + +  ENQ+  ELR +   +C  W+  I  AS+  L  + E L QK+LHLLQ+VE+EKT   Q  Q  ++ + EI+ ++ E+ +L K+   + Q +++ +  D       ED DI KIKKVQSF RGWLCRR+WK I+++YIRSPHA+SMRKRN +VF M+EAE EYV+QL IL++ FLRP +MAASSK PP  H+D++SIFLNSET++FLHQIF +GL AR+ SWPTLVL     DLFD+LLPML+IYQE+VRNH Y LQ+L  CKQ   F  LL   E KP C+ R+LETFLTYPM QIPRYI+TLHELLAHTP +HVE+ SL++A+ +LE+LSR MHDEVSETENIRKNLAIERMIIEGC+ILLD +Q FVRQG+L+Q+  +      RGRL ++  +KE E  RQCFLFS HLI+ TR   G+LHL    G I L D TL+E+P   E     E   GS              +H  +DFK+ ++  K     T+ L+A + QEKA+W +DI+QC+D +  NGL  N     S  T+PQ +K             SD +L+ DDVDIRFSKT N+CK+ QIRYA+ ERLL+RLTDLRFL+IDFLNTFL +YRVFT  I VL+ L T++  P
Sbjct:   84 SAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEITSLLKDNERI-QSTQTVAPND-------EDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLA----DLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGE--RQCFLFSKHLIICTRGSGGKLHLTKN-GVISLIDCTLLEEPESTE-----EEAKGSGQD----------IDH--LDFKIGVEP-KDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKRTREGTREAEMSRSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKP 698          

HSP 2 Score: 306.605 bits (784), Expect = 7.024e-84
Identity = 138/233 (59.23%), Postives = 177/233 (75.97%), Query Frame = 0

Query: 1572 SALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPR 1804
            SALEIAEQ+  LD  +   I   EF GQ W K +K+ R+P+I+  TK FN++S L+ S+I+   D+  RV  IEKW AVADICRCLHN+N VL+I ++   SA+FRLK TW +VSK +K  I+KLQ LVSS+GRF+NLR+AL   DPPC+PYLGMYL+DL FIEEGTPN+T++ L+NF+KMRMI+H+IREIRQFQQT YKIE   KVT Y+LD    +D+E LY+ SL +EP+
Sbjct: 1038 SALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPK 1270          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. SwissProt
Match: gi|74851638|sp|Q54FF3.1|GEFK_DICDI (RecName: Full=Ras guanine nucleotide exchange factor K; AltName: Full=RasGEF domain-containing protein K)

HSP 1 Score: 181.415 bits (459), Expect = 2.143e-44
Identity = 96/240 (40.00%), Postives = 144/240 (60.00%), Query Frame = 0

Query: 1575 EIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVR-SPHIVIMTKRFNEMSRLVVSDIVTQPD-VGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSSF 1812
            EIA Q+   D ++   I+ +EFL QAW KP    R SP I+ +  RFN++S  VVS ++ +PD V  R + +++  ++AD  R L+N+N  + + +   NSA+ RLK T   +SK     +E L+  +S +G ++N RD L  +DPP +PY+G+YL+DLTFIEEG PN    +L+NFAK  +I  VI EI+Q+Q T Y++   P +  +I D       + LY  SL+ EPR +  S  F
Sbjct: 1319 EIARQLTLYDFQLYTAIKPTEFLNQAWNKPSMASRKSPTILKIISRFNDISLWVVS-LILEPDRVKTRAKRLKRIISIADELRKLNNYNTCIAVISGINNSAILRLKYTRGLLSKKYLDILENLEKEMSCEGSYKNYRDKLKNSDPPVVPYIGVYLTDLTFIEEGNPNIIRGNLINFAKYYLIYRVISEIQQYQWTEYQLNVAPIIQTFIRDVSISSTSDDLYHLSLLKEPRNALKSDIF 1557          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. SwissProt
Match: gi|259647520|sp|B0M0P8.1|GEFL_DICDI (RecName: Full=Ras guanine nucleotide exchange factor L; AltName: Full=RasGEF domain-containing protein L)

HSP 1 Score: 171.014 bits (432), Expect = 4.498e-41
Identity = 90/241 (37.34%), Postives = 148/241 (61.41%), Query Frame = 0

Query: 1574 LEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKI-----EQVPKVTNYILDPERQLD---DEMLYQQSLIVEPRTS 1806
            L +A+Q+   + +    IQ+ EFLGQAW KP    ++P+++ M +RFN++S  V + I++QP +  RV++I ++  +A  CR L+N++ +    A   NS V RL+ +W +V K     +E L+ ++S +G F+  R  +  T PPCIPYLG+YL DLTFIEEG P+ + N L+N+ K ++I ++I  I++ QQ  Y        QV + ++ +L+    L    DE+LY+ SL +EPR++
Sbjct: 2114 LFVAQQLTLREYETFKRIQAVEFLGQAWGKPKLQYKAPNLLKMIERFNKISTGVSTAILSQPKIKTRVKLICRFIKIAQHCRDLNNYHLLTAFMAGIRNSNVVRLRLSWAKVPKKHLHILEDLEKIMSMEGSFKEFRTKMAETIPPCIPYLGVYLKDLTFIEEGNPD-SINSLINWGKKKLIYNIISIIQRCQQVSYDFGNQNQSQVAQKSDLVLNYFDSLPIALDELLYKISLNLEPRSN 2353          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. SwissProt
Match: gi|74843130|sp|Q8IS14.1|GEFJ_DICDI (RecName: Full=Ras guanine nucleotide exchange factor J; AltName: Full=RasGEF domain-containing protein J)

HSP 1 Score: 166.777 bits (421), Expect = 1.352e-40
Identity = 87/239 (36.40%), Postives = 142/239 (59.41%), Query Frame = 0

Query: 1567 NINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADIC-RCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPR 1804
            +I S+   EIA Q+  +D +I   I+S+E L Q+W KP    RSP+++ +  RFNE+S+   + I++ P V  R +++ K+  +A+ C R L+NFN  + I +    S+V RLK T   + K ++Q   +LQ  +SS   ++  R  L + +PPC+PYLG+YL+DLTF EEG P+F     +NF K ++I   I  ++ FQ   Y ++ V ++T  +L   + L++  LY +S+  EPR
Sbjct:  568 DIFSVDEEEIARQLTLMDFEIFSAIKSTELLNQSWNKPKLRHRSPNVLTLINRFNEISQWTATSILSYPKVKDRARIMAKFIKIAEYCMRHLNNFNTSMAILSGLNASSVHRLKFTKEELPKHTQQVYTELQFHLSSAQAYKEYRALLAKANPPCLPYLGVYLTDLTFFEEGNPDFIQG-FINFGKRKLIYGSISNVQSFQNAKYNLQPVYQITK-LLKGFKLLEENDLYSRSMSFEPR 804          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. nr
Match: gi|972202778|ref|XP_015183519.1| (PREDICTED: ras-specific guanine nucleotide-releasing factor 2-like isoform X1 [Polistes dominula])

HSP 1 Score: 952.584 bits (2461), Expect = 0.000e+0
Identity = 601/1234 (48.70%), Postives = 730/1234 (59.16%), Query Frame = 0

Query:  171 YCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGR--------------------------------LLPQISKS-SSSQDGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQIL--PAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRT--QAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPE-----SPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFF--NPDQGSSPSSSNGSMENL--THAEGSIQVNLLHPSQSAAADGSRRISTCSGYSTTTDNGRHGSLTPSRRISGASSLSGYYSDERERSSQ---DSN------HKSQHWRYSYRRYEEEQRNEDAASSVTGTRHGMPRGSIGLL---PSSRKGSHDSTASGRSGSIISNAQWRKESVTIVEQVEPIHEQDDATSDRGDLAGTRRQNSLGMAEAPSQAVAKTTSLGSDGGTEEKFLAIPKKPITASSSAETLTDNT-LTAP----------------------PSPKSGGTPSPG--RNAPGPPQRRASHVDQL------VSVQSPSSMEDNEVDRPETTPLKSALKKCSISSITS---------GPTPKSRGRSTSTFSSASYSYQVNNDDDGSDLIAARGCSSQPGSHHPSFSSPTGSRIIFGGLNLKDLTTSMKSPTKEHKKKKGIFNRHIASATSNDSSNDHSSSPISSPQHSKMGVVVTSSRQAHRR-------SSSATAAVAFAVATAGSGNPPDPTNKDVFKEAA----------KRKMSLTSSVSTMRVLSVLRHWVTKHAQ 1289
            +CFAI YRRENQR+YEL+A T ++C+ WID I+ ASFNKL LQ EELEQKHLHLLQ+VESEKTAKWQYTQ C+EL  EIK +R EL  LKKE R                                L P+I  S  S   G +   E   ++ KIKKVQSFFRGWLCRRRWKQIVE+YI+SPHAESMRKRN LVF+MVEAEEEY EQ+EIL+SCFLRPFKMAASSK PPC+HED+NSIFLNSETVLFLHQIFLKGLT+RMESWPTLVLG    DLFD+LLPMLSIYQEYVRNHHY LQVLTECKQ   F  LL+RLE K AC GRSLE FLTYPMHQIPRYIITLHELLAHTP DHVE+KSL++AR QLEDLSRQMHDEVSETEN+RKNLA+ERMI+EGCDILLDVNQ+FVRQG LVQIL  P G++S            ++EAVRQCFLFSNHL++TTR     GRL+L+  +GKIPL+D  L+EDP E+   D+ E       SG S+S   S  GNT  + ++ DFK++LD    G  +T+HL+APT QEKA+WI+DI+QCMD VHFN +F+ +M + SS T+PQ +K+DP LFKDDVDIRFS+T N+CKIPQIR ATPERLLQRLTDLRFL+IDFLNTFLLTYRVFT+G+TVLEALK VF+   P     P  S  S++ L  T +EG                            T TD  R  S    RR SGASS+SGY S+  ER      +SN      +   HWR   +R EEEQ                   S+GL+   PS  K S    +S  S +  S+A  RK S+ +          D+ T + G                                     L IP K I  SSS+ETLT+ T L+AP                      PSP+ GGT S G       P     S  D++      V++ S  S +    ++  +TP      K     I             TP S G+S +T  S    YQ                      HH    +   S  +  G+    ++T M +       ++ I    + S++  +  +D  +S       SK GVVVTS R++HR        SS++TAA AFA+AT+ S NPPD   K ++K +           +RK S+ S+ +TMRVL+VLRHWV+KHAQ
Sbjct:   93 HCFAITYRRENQRQYELKASTETDCKQWIDAIREASFNKLLLQKEELEQKHLHLLQIVESEKTAKWQYTQQCEELASEIKKLRAELCTLKKELRPSVAQGFTTRPGFQRTLSQGTGSLASGLVHYGLYPEIQGSLVSCSLGLRGITEGSVEMQKIKKVQSFFRGWLCRRRWKQIVEQYIKSPHAESMRKRNSLVFQMVEAEEEYTEQMEILVSCFLRPFKMAASSKKPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRMESWPTLVLG----DLFDILLPMLSIYQEYVRNHHYSLQVLTECKQSSPFVALLNRLENKTACHGRSLEMFLTYPMHQIPRYIITLHELLAHTPHDHVERKSLQNARQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGALVQILDKPRGARSRLSATFGGKGSSDKEAVRQCFLFSNHLLLTTRQSDDDGRLNLIPHIGKIPLSDAVLIEDPCEQGVTDDDEFSVCSMSSGISESCG-SASGNTSSQLQNRDFKIVLDVKSGGPLVTVHLVAPTIQEKAAWISDISQCMDNVHFNDIFHCSMSDTSSVTMPQSIKNDPKLFKDDVDIRFSRTLNSCKIPQIRSATPERLLQRLTDLRFLSIDFLNTFLLTYRVFTDGVTVLEALKKVFYETEPPDAQMPCGSLVSLDVLGGTMSEG---------------------------QTHTDERRRSS-VSPRRTSGASSVSGYGSEISERDRHYMYESNTGAHTLNSKTHWRGGCKRLEEEQ-------------------SLGLITEAPSIMKHSPTLQSSNPSVTTQSSATSRKISIRV----------DNNTENDG-----------------------------------CHLTIP-KVIAGSSSSETLTEATVLSAPSSPSNLSTVTLIGSTGSGSGSDPSPQEGGTISRGISEETDTPVATSTSSKDEVQFTYADVTLTSGKSTDSETTEKTPSTPATPQTPKIKDHEIEKETRKEWTEEEKTPVSIGQSQTT--SIQTKYQ---------------------QHHMEHRASVASAPV-TGMRSGSISTVMGN---HWATRRSIQESDLCSSSQGNFQHDQQAS-------SKAGVVVTSFRRSHRSIGPQPIWSSTSTAATAFAIATSASSNPPD---KVLWKNSVSDGSNRNDKNRRKESVMSTAATMRVLNVLRHWVSKHAQ 1191          

HSP 2 Score: 385.956 bits (990), Expect = 2.467e-106
Identity = 185/278 (66.55%), Postives = 220/278 (79.14%), Query Frame = 0

Query: 1534 EEPEESKGKKSGTLKPSASSGALNGLRQVPSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSS 1811
            EEPE +K      L P            V SKE+I +LSALEIAEQM +LD +I  +I S EFLGQAW K DK  R+PHI++MTKRFNE+S+LVVS+I+ + ++  RV  IEKW AVADI R LHN+NGVLQICAAFTNS+V+RLK TW +VSK++KQTIEKLQ +VSSDGRFRNLRDALHR DPPCIPYLG+YL+DL+FIEEGTPNFT+  LLNF+KMRMIAHVIREIR FQQTPYKIE + KVTNY+LD    L+++ LY+ SL +EPR SRLSSS
Sbjct: 1234 EEPESNKVDLKKLLTPPT----------VASKESIETLSALEIAEQMTYLDHQIFISITSEEFLGQAWMKTDKATRAPHILLMTKRFNEVSQLVVSEIIRRSNMSARVAAIEKWAAVADISRVLHNYNGVLQICAAFTNSSVYRLKKTWEKVSKTTKQTIEKLQNIVSSDGRFRNLRDALHRCDPPCIPYLGLYLTDLSFIEEGTPNFTEEGLLNFSKMRMIAHVIREIRHFQQTPYKIELITKVTNYLLDSSLLLNEKDLYRMSLEIEPRASRLSSS 1501          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. nr
Match: gi|972202780|ref|XP_015183520.1| (PREDICTED: ras-specific guanine nucleotide-releasing factor 2-like isoform X2 [Polistes dominula])

HSP 1 Score: 951.429 bits (2458), Expect = 0.000e+0
Identity = 599/1230 (48.70%), Postives = 727/1230 (59.11%), Query Frame = 0

Query:  171 YCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGR--------------------------------LLPQISKS-SSSQDGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQIL--PAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRT--QAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSL-SDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFF--NPDQGSSPSSSNGSMENL--THAEGSIQVNLLHPSQSAAADGSRRISTCSGYSTTTDNGRHGSLTPSRRISGASSLSGYYSDERERSSQ---DSN------HKSQHWRYSYRRYEEEQRNEDAASSVTGTRHGMPRGSIGLL---PSSRKGSHDSTASGRSGSIISNAQWRKESVTIVEQVEPIHEQDDATSDRGDLAGTRRQNSLGMAEAPSQAVAKTTSLGSDGGTEEKFLAIPKKPITASSSAETLTDNT-LTAP----------------------PSPKSGGTPSPG--RNAPGPPQRRASHVDQL------VSVQSPSSMEDNEVDRPETTPLKSALKKCSISSITS---------GPTPKSRGRSTSTFSSASYSYQVNNDDDGSDLIAARGCSSQPGSHHPSFSSPTGSRIIFGGLNLKDLTTSMKSPTKEHKKKKGIFNRHIASATSNDSSNDHSSSPISSPQHSKMGVVVTSSRQAHRR-------SSSATAAVAFAVATAGSGNPPDPTNKDVFKEAA----------KRKMSLTSSVSTMRVLSVLRHWVTKHAQ 1289
            +CFAI YRRENQR+YEL+A T ++C+ WID I+ ASFNKL LQ EELEQKHLHLLQ+VESEKTAKWQYTQ C+EL  EIK +R EL  LKKE R                                L P+I  S  S   G +   E   ++ KIKKVQSFFRGWLCRRRWKQIVE+YI+SPHAESMRKRN LVF+MVEAEEEY EQ+EIL+SCFLRPFKMAASSK PPC+HED+NSIFLNSETVLFLHQIFLKGLT+RMESWPTLVLG    DLFD+LLPMLSIYQEYVRNHHY LQVLTECKQ   F  LL+RLE K AC GRSLE FLTYPMHQIPRYIITLHELLAHTP DHVE+KSL++AR QLEDLSRQMHDEVSETEN+RKNLA+ERMI+EGCDILLDVNQ+FVRQG LVQIL  P G++S            ++EAVRQCFLFSNHL++TTR     GRL+L+  +GKIPL+D  L+EDP E+   D+      S  S S  S  GNT  + ++ DFK++LD    G  +T+HL+APT QEKA+WI+DI+QCMD VHFN +F+ +M + SS T+PQ +K+DP LFKDDVDIRFS+T N+CKIPQIR ATPERLLQRLTDLRFL+IDFLNTFLLTYRVFT+G+TVLEALK VF+   P     P  S  S++ L  T +EG                            T TD  R  S    RR SGASS+SGY S+  ER      +SN      +   HWR   +R EEEQ                   S+GL+   PS  K S    +S  S +  S+A  RK S+ +          D+ T + G                                     L IP K I  SSS+ETLT+ T L+AP                      PSP+ GGT S G       P     S  D++      V++ S  S +    ++  +TP      K     I             TP S G+S +T  S    YQ                      HH    +   S  +  G+    ++T M +       ++ I    + S++  +  +D  +S       SK GVVVTS R++HR        SS++TAA AFA+AT+ S NPPD   K ++K +           +RK S+ S+ +TMRVL+VLRHWV+KHAQ
Sbjct:   93 HCFAITYRRENQRQYELKASTETDCKQWIDAIREASFNKLLLQKEELEQKHLHLLQIVESEKTAKWQYTQQCEELASEIKKLRAELCTLKKELRPSVAQGFTTRPGFQRTLSQGTGSLASGLVHYGLYPEIQGSLVSCSLGLRGITEGSVEMQKIKKVQSFFRGWLCRRRWKQIVEQYIKSPHAESMRKRNSLVFQMVEAEEEYTEQMEILVSCFLRPFKMAASSKKPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRMESWPTLVLG----DLFDILLPMLSIYQEYVRNHHYSLQVLTECKQSSPFVALLNRLENKTACHGRSLEMFLTYPMHQIPRYIITLHELLAHTPHDHVERKSLQNARQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGALVQILDKPRGARSRLSATFGGKGSSDKEAVRQCFLFSNHLLLTTRQSDDDGRLNLIPHIGKIPLSDAVLIEDPCEQGVTDDDVCSMSSGISESCGSASGNTSSQLQNRDFKIVLDVKSGGPLVTVHLVAPTIQEKAAWISDISQCMDNVHFNDIFHCSMSDTSSVTMPQSIKNDPKLFKDDVDIRFSRTLNSCKIPQIRSATPERLLQRLTDLRFLSIDFLNTFLLTYRVFTDGVTVLEALKKVFYETEPPDAQMPCGSLVSLDVLGGTMSEG---------------------------QTHTDERRRSS-VSPRRTSGASSVSGYGSEISERDRHYMYESNTGAHTLNSKTHWRGGCKRLEEEQ-------------------SLGLITEAPSIMKHSPTLQSSNPSVTTQSSATSRKISIRV----------DNNTENDG-----------------------------------CHLTIP-KVIAGSSSSETLTEATVLSAPSSPSNLSTVTLIGSTGSGSGSDPSPQEGGTISRGISEETDTPVATSTSSKDEVQFTYADVTLTSGKSTDSETTEKTPSTPATPQTPKIKDHEIEKETRKEWTEEEKTPVSIGQSQTT--SIQTKYQ---------------------QHHMEHRASVASAPV-TGMRSGSISTVMGN---HWATRRSIQESDLCSSSQGNFQHDQQAS-------SKAGVVVTSFRRSHRSIGPQPIWSSTSTAATAFAIATSASSNPPD---KVLWKNSVSDGSNRNDKNRRKESVMSTAATMRVLNVLRHWVSKHAQ 1188          

HSP 2 Score: 385.956 bits (990), Expect = 2.623e-106
Identity = 185/278 (66.55%), Postives = 220/278 (79.14%), Query Frame = 0

Query: 1534 EEPEESKGKKSGTLKPSASSGALNGLRQVPSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSS 1811
            EEPE +K      L P            V SKE+I +LSALEIAEQM +LD +I  +I S EFLGQAW K DK  R+PHI++MTKRFNE+S+LVVS+I+ + ++  RV  IEKW AVADI R LHN+NGVLQICAAFTNS+V+RLK TW +VSK++KQTIEKLQ +VSSDGRFRNLRDALHR DPPCIPYLG+YL+DL+FIEEGTPNFT+  LLNF+KMRMIAHVIREIR FQQTPYKIE + KVTNY+LD    L+++ LY+ SL +EPR SRLSSS
Sbjct: 1231 EEPESNKVDLKKLLTPPT----------VASKESIETLSALEIAEQMTYLDHQIFISITSEEFLGQAWMKTDKATRAPHILLMTKRFNEVSQLVVSEIIRRSNMSARVAAIEKWAAVADISRVLHNYNGVLQICAAFTNSSVYRLKKTWEKVSKTTKQTIEKLQNIVSSDGRFRNLRDALHRCDPPCIPYLGLYLTDLSFIEEGTPNFTEEGLLNFSKMRMIAHVIREIRHFQQTPYKIELITKVTNYLLDSSLLLNEKDLYRMSLEIEPRASRLSSS 1498          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. nr
Match: gi|939249984|ref|XP_014244538.1| (ras-specific guanine nucleotide-releasing factor 2-like isoform X2 [Cimex lectularius])

HSP 1 Score: 949.503 bits (2453), Expect = 0.000e+0
Identity = 489/747 (65.46%), Postives = 570/747 (76.31%), Query Frame = 0

Query:  171 YCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGRLL---------PQISKSSSSQDGDKSEDEEDP-DIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSG-NQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNPDQGSSPSSSNGSMENLTHAEGSIQVNLLHPSQSAAADGSRRISTCSGYSTTTDNGRHGSLTPSRRISGASSLSGYYSD--ERERS-SQDSNHKSQ-HWRYSYRRYEEEQRN 902
            YCFAI YR E+QR+YELRA T S+C+AWI+ IK ASFNKL LQ EELEQKHLHLLQ+VESEKTAKWQYT  C+EL  EIK +R EL ALKKE R +         P I   S + D +  +  ED  ++ KIKKVQSFFRGWLCRRRWKQIVE+YI+SPHAESMRKRN LVFRMVEAEEEY+EQLE+L+SCFLRPFKMAASSK PPC+HED+NSIFLNSETVLFLHQIFLKGLT+RMESWPTLVLG    DLFDMLLPMLSIYQEYVRNHHY LQVLTECKQ PQF +LL RLE KP C+GRSLE FLTYPMHQIPRYIITLHELLAHTP DHVE+KSL++A+ QLEDLSRQMHDEVSETEN+RKNLA+ERMI+EGCDILLDVNQ+FVRQG+L+QI P  +K GAR RL    + E+EA+RQCFLFSNHLI+ TRT AGRLHLV  VGKIPLAD  L+EDPSE    ++ ES + S  S S      T    ++ DFK++L+   S   Q+ +HLIAPT QEKA+WI+DI+QCMD VHFN L ++++ + SS T+PQ +K+DP LFKDDVDIRFS+T N+CK+PQIRYATP RLL+RLTDLRFL+IDFLNTFLLTYRVFT+G+TVLEALK VF+N D                            P  +   +    +ST   Y    D+ R  S    RR SGASS+SGY S+  +R+RS S DS    + HWR S+R++EEE  N
Sbjct:   96 YCFAISYRIESQRQYELRASTESDCKAWIEAIKEASFNKLLLQKEELEQKHLHLLQIVESEKTAKWQYTLQCEELASEIKKLRAELCALKKEWRPMRPLGGLCKAPSIVGGSGTADANNIQYPEDSMELRKIKKVQSFFRGWLCRRRWKQIVEQYIKSPHAESMRKRNSLVFRMVEAEEEYMEQLEVLVSCFLRPFKMAASSKKPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRMESWPTLVLG----DLFDMLLPMLSIYQEYVRNHHYSLQVLTECKQTPQFASLLARLENKPTCQGRSLEIFLTYPMHQIPRYIITLHELLAHTPHDHVERKSLQNAKQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGSLIQIPPEKAK-GARNRLP-NFKSEKEALRQCFLFSNHLILATRTSAGRLHLVPEVGKIPLADAVLIEDPSENGVNEDDESSTCSMSSHSSGISETTTGSLQNRDFKIVLEGKSSSQQQVAVHLIAPTIQEKAAWISDISQCMDNVHFNDLLHSSLSDTSSVTMPQSIKNDPKLFKDDVDIRFSRTLNSCKVPQIRYATPGRLLERLTDLRFLSIDFLNTFLLTYRVFTDGVTVLEALKKVFYNADP---------------------------PDAADIPERDDTLSTLHLY----DSERRRSSASPRRTSGASSVSGYGSEASDRDRSHSYDSQSFPRGHWRCSFRKFEEEMTN 805          

HSP 2 Score: 384.415 bits (986), Expect = 3.233e-106
Identity = 182/249 (73.09%), Postives = 219/249 (87.95%), Query Frame = 0

Query: 1563 PSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSS 1811
            PSKE+I +LSALEIAEQM ++D +I  +I+S EFLGQAW K DK  ++PHI++MT+RFNE+S+LVVS+I+ +P++  R+  IEKW AVADI RCLHNFNGVLQ+CAAFTNS+VFRLK TW +VSK++KQTIEKLQ +VSSDGRFRNLRDALHR DPPCIPYLGMYL+DL+FIEEGTPNFT++ LLNF+KMRMIAHVIREIR+FQQTPYKIE + KVTNY+LDP   LDD+ LYQ SL +EPRTSRLS+S
Sbjct: 1146 PSKESIETLSALEIAEQMTYVDHQIFVSIRSEEFLGQAWMKTDKATKAPHIILMTRRFNEVSQLVVSEIIRRPNMTARIHAIEKWAAVADISRCLHNFNGVLQVCAAFTNSSVFRLKKTWEKVSKTTKQTIEKLQTIVSSDGRFRNLRDALHRCDPPCIPYLGMYLTDLSFIEEGTPNFTEDGLLNFSKMRMIAHVIREIRRFQQTPYKIELITKVTNYLLDPALLLDDDELYQMSLEIEPRTSRLSAS 1394          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. nr
Match: gi|1009379618|gb|KYN02681.1| (Ras-specific guanine nucleotide-releasing factor 2, partial [Cyphomyrmex costatus])

HSP 1 Score: 949.118 bits (2452), Expect = 0.000e+0
Identity = 583/1203 (48.46%), Postives = 716/1203 (59.52%), Query Frame = 0

Query:  171 YCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGR--LLPQISKSSSSQD----------------------------GDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQIL--PAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRT--QAGRLHLVDGVGKIPLADVTLVED---PSEEERMDEPESPSGSSDSSSLSDMGNTRYEHKSI---DFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFF--NPDQGSSPSSSNGSMENLTHAEGSIQVNLLHPSQSAAADGSRRISTCSGYSTTTDNGRHGSLTPSRRISGASSLSGY---YSDERERSSQDS--------NHKSQHWRYSYRRYEEEQRNEDAASSVTGTRHGMPRGSIGLLPSSRKGSHDSTASGRSGSIISNAQWRKESVTIVEQVEPIHEQDDATSDRGDLAGTRRQNSLGMAEAPSQAVAKTTSLGSDGGTEEKFLAIPKKPITASSSAETLTDNT-LTAPPSPKSGGT------------------PSPGRNAPGPPQRRASHVDQLVSVQSPSSMEDNEVDRPETTPLKSALKKCSISSITSGP-TPKSRGRSTSTFSSASYSYQVNNDDDGSDLIAARGCSSQPGSHHPSFSSPTGSRIIFGGLNLKDLTTSMKSPTKEHKKKKGIFNRHIASATSNDSSNDHSSSPISSPQHSKMGVVVTSSRQAHRR-------SSSATAAVAFAVATAGSGNPPDP----TNKDVFKEAAKRKMSLTSSVSTMRVLSVLRHWVTKHAQ 1289
            +CFAI YRRENQR+YEL+A T ++C+AWID I+ ASFNKL LQ EELEQKHLHLLQ+VESEKTAKWQYTQ C+EL  EIK +R EL ALKKE R  ++P  +   + Q                             G +   E   ++ K+KKVQSFFRGWLCRRRWKQIVE+YI+SPHAESMRKRN LVF+MVEAEEEY EQ+EIL+SCFLRPFKMAASSK PPC+HED+NSIFLNSETVLFLHQIFLKGLT+RMESWPTLVLG    DLFDMLLPMLSIYQEYVRNHHY LQVLTECKQ   F  LL+RLE K AC GRSLE FLTYPMHQIPRYIITLHELLAHTP DHVE+KSL++AR QLEDLSRQMHDEVSETEN+RKNLA+ERMI+EGCDILLDVNQ+FVRQGTL+Q++  P G++S            ++EAVRQCFLFSNHL++TTR     GRL+LV  +GKIPL+D  L+ED   PS + ++    S    S   S S    +      +   DFK+ILD    G Q+T+HL+APT QEKA+W++DI+QCMD VHFN L + ++ + SS T+PQ +++DP LFKDDVDIRFS+T N+CKIPQIR ATPERLLQRLTDLRFL+IDFLNTFLLTYRVFT+G+TVLEALK VF+   P      + S  S++ L      IQ+                      YS   ++ R  S +P RR SGASS+SGY    SD R+  S  S        N KS HWR  Y++ +EEQ                P G I   P  ++    S AS  S S+ S A  RK S+ +               D  D+                           DG      L IPK  I  SSS+ETLTD T ++AP SP +  +                  PSP     G   RR S +D      +P + E++   +P       + K          P  PK+     S     +   Q  + DD   + + +  +     +   +S                          EH+      NR     + +D  +   SS   +   SK GVV+TS RQ+HR        SS++TAA AFA+AT+ S NPPD        +  ++  +RK S+ S+ +TMRVL+VLRHWV+KHAQ
Sbjct:    1 HCFAISYRRENQRQYELKAATETDCKAWIDAIREASFNKLLLQKEELEQKHLHLLQIVESEKTAKWQYTQQCEELASEIKKLRAELCALKKELRPSVIPIYANRPAVQRAMSQGTASLYSTFHSYGGGAHSSGVFAPGLRDISEGSIEMQKVKKVQSFFRGWLCRRRWKQIVEQYIKSPHAESMRKRNSLVFQMVEAEEEYTEQMEILVSCFLRPFKMAASSKKPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRMESWPTLVLG----DLFDMLLPMLSIYQEYVRNHHYSLQVLTECKQSSPFVALLNRLENKTACHGRSLEIFLTYPMHQIPRYIITLHELLAHTPYDHVERKSLQNARQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGTLIQLVDKPRGARSRLSATFGGKAASDKEAVRQCFLFSNHLLLTTRQSDDDGRLNLVAQIGKIPLSDAVLIEDPMLPSSDCKLILGLSVCSMSSGISESSGSGSGSGTSQLQNRDFKIILDIKSGGPQVTVHLVAPTMQEKAAWVSDISQCMDNVHFNDLLHGSLSDTSSVTMPQSIRNDPKLFKDDVDIRFSRTLNSCKIPQIRSATPERLLQRLTDLRFLSIDFLNTFLLTYRVFTDGVTVLEALKKVFYEAEPPDAQMTTGSLVSLDVLGTNANEIQL----------------------YS---EDRRKSSFSP-RRTSGASSVSGYGSEVSDTRDAKSHGSFDANTSVYNSKS-HWRSGYKKLDEEQ----------------PLGLISEAPVIKRIP-TSQASSPSNSLQSPAMPRKISIRV---------------DNNDVEN-------------------------DGC----HLTIPKV-IAVSSSSETLTDVTAISAPSSPSNLSSVTLVGSTESGSGSGSGSDPSPQEG--GTYSRRVSEID------TPGTAEEDAKAKPAKPDTPKSTKTPDTPRTPKTPDVPKTPKTPISPLIKENKDEQSTSTDDEESITSPKPNNQSIQQNQQQYS--------------------------EHRASNYWVNRR----SMHDEISSQQSSYHDAQVSSKAGVVITSFRQSHRSIGPEPIWSSTSTAATAFAIATSASSNPPDKGPATDGNNRGRDKNRRKESVMSTAATMRVLNVLRHWVSKHAQ 1072          

HSP 2 Score: 371.703 bits (953), Expect = 4.572e-102
Identity = 175/251 (69.72%), Postives = 212/251 (84.46%), Query Frame = 0

Query: 1561 QVPSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSS 1811
            ++ SKE+I +LSALEIAEQM +LD  I  +I S EFLGQAW K +K  R+PHI++MTKRFNE+S+LVVS+I+ + ++  RV  IEKW+AVADI R LHN+NGVLQICAAFTNS+V+RLK TW++VSK++KQTIE+LQ +VSSDGRFRNLRDALHR DPPCIPYLG+YL+DL+FIEEGTPN T+  LLNF+KMRMI+HVIREIR FQQTPYKIE V KVTNY+LD    L +  LY+ SL +EPRTSRLSSS
Sbjct: 1132 EISSKESIETLSALEIAEQMTYLDHHIFVSIASEEFLGQAWMKMEKATRAPHILLMTKRFNEVSQLVVSEIIRRSNMSARVAAIEKWSAVADINRVLHNYNGVLQICAAFTNSSVYRLKKTWDKVSKTTKQTIERLQHIVSSDGRFRNLRDALHRCDPPCIPYLGLYLTDLSFIEEGTPNVTEG-LLNFSKMRMISHVIREIRHFQQTPYKIELVTKVTNYLLDTSLLLSERDLYRMSLELEPRTSRLSSS 1381          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. nr
Match: gi|939249982|ref|XP_014244537.1| (ras-specific guanine nucleotide-releasing factor 2-like isoform X1 [Cimex lectularius])

HSP 1 Score: 948.732 bits (2451), Expect = 0.000e+0
Identity = 489/747 (65.46%), Postives = 570/747 (76.31%), Query Frame = 0

Query:  171 YCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGRLL---------PQISKSSSSQDGDKSEDEEDP-DIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSG-NQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNPDQGSSPSSSNGSMENLTHAEGSIQVNLLHPSQSAAADGSRRISTCSGYSTTTDNGRHGSLTPSRRISGASSLSGYYSD--ERERS-SQDSNHKSQ-HWRYSYRRYEEEQRN 902
            YCFAI YR E+QR+YELRA T S+C+AWI+ IK ASFNKL LQ EELEQKHLHLLQ+VESEKTAKWQYT  C+EL  EIK +R EL ALKKE R +         P I   S + D +  +  ED  ++ KIKKVQSFFRGWLCRRRWKQIVE+YI+SPHAESMRKRN LVFRMVEAEEEY+EQLE+L+SCFLRPFKMAASSK PPC+HED+NSIFLNSETVLFLHQIFLKGLT+RMESWPTLVLG    DLFDMLLPMLSIYQEYVRNHHY LQVLTECKQ PQF +LL RLE KP C+GRSLE FLTYPMHQIPRYIITLHELLAHTP DHVE+KSL++A+ QLEDLSRQMHDEVSETEN+RKNLA+ERMI+EGCDILLDVNQ+FVRQG+L+QI P  +K GAR RL    + E+EA+RQCFLFSNHLI+ TRT AGRLHLV  VGKIPLAD  L+EDPSE    ++ ES + S  S S      T    ++ DFK++L+   S   Q+ +HLIAPT QEKA+WI+DI+QCMD VHFN L ++++ + SS T+PQ +K+DP LFKDDVDIRFS+T N+CK+PQIRYATP RLL+RLTDLRFL+IDFLNTFLLTYRVFT+G+TVLEALK VF+N D                            P  +   +    +ST   Y    D+ R  S    RR SGASS+SGY S+  +R+RS S DS    + HWR S+R++EEE  N
Sbjct:   96 YCFAISYRIESQRQYELRASTESDCKAWIEAIKEASFNKLLLQKEELEQKHLHLLQIVESEKTAKWQYTLQCEELASEIKKLRAELCALKKEWRPMRPLGGLCKAPSIVGGSGTADANNIQYPEDSMELRKIKKVQSFFRGWLCRRRWKQIVEQYIKSPHAESMRKRNSLVFRMVEAEEEYMEQLEVLVSCFLRPFKMAASSKKPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRMESWPTLVLG----DLFDMLLPMLSIYQEYVRNHHYSLQVLTECKQTPQFASLLARLENKPTCQGRSLEIFLTYPMHQIPRYIITLHELLAHTPHDHVERKSLQNAKQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGSLIQIPPEKAK-GARNRLP-NFKSEKEALRQCFLFSNHLILATRTSAGRLHLVPEVGKIPLADAVLIEDPSENGVNEDDESSTCSMSSHSSGISETTTGSLQNRDFKIVLEGKSSSQQQVAVHLIAPTIQEKAAWISDISQCMDNVHFNDLLHSSLSDTSSVTMPQSIKNDPKLFKDDVDIRFSRTLNSCKVPQIRYATPGRLLERLTDLRFLSIDFLNTFLLTYRVFTDGVTVLEALKKVFYNADP---------------------------PDAADIPERDDTLSTLHLY----DSERRRSSASPRRTSGASSVSGYGSEASDRDRSHSYDSQSFPRGHWRCSFRKFEEEMTN 805          

HSP 2 Score: 384.03 bits (985), Expect = 4.429e-106
Identity = 182/249 (73.09%), Postives = 219/249 (87.95%), Query Frame = 0

Query: 1563 PSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSS 1811
            PSKE+I +LSALEIAEQM ++D +I  +I+S EFLGQAW K DK  ++PHI++MT+RFNE+S+LVVS+I+ +P++  R+  IEKW AVADI RCLHNFNGVLQ+CAAFTNS+VFRLK TW +VSK++KQTIEKLQ +VSSDGRFRNLRDALHR DPPCIPYLGMYL+DL+FIEEGTPNFT++ LLNF+KMRMIAHVIREIR+FQQTPYKIE + KVTNY+LDP   LDD+ LYQ SL +EPRTSRLS+S
Sbjct: 1153 PSKESIETLSALEIAEQMTYVDHQIFVSIRSEEFLGQAWMKTDKATKAPHIILMTRRFNEVSQLVVSEIIRRPNMTARIHAIEKWAAVADISRCLHNFNGVLQVCAAFTNSSVFRLKKTWEKVSKTTKQTIEKLQTIVSSDGRFRNLRDALHRCDPPCIPYLGMYLTDLSFIEEGTPNFTEDGLLNFSKMRMIAHVIREIRRFQQTPYKIELITKVTNYLLDPALLLDDDELYQMSLEIEPRTSRLSAS 1401          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. nr
Match: gi|972202782|ref|XP_015183521.1| (PREDICTED: ras-specific guanine nucleotide-releasing factor 2-like isoform X3 [Polistes dominula])

HSP 1 Score: 947.962 bits (2449), Expect = 0.000e+0
Identity = 602/1227 (49.06%), Postives = 731/1227 (59.58%), Query Frame = 0

Query:  171 YCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGR--------------------------------LLPQISKS-SSSQDGDKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQIL--PAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRT--QAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPE-----SPSGSSDSSSLSDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFF--NPDQGSSPSSSNGSMENL--THAEGSIQVNLLHPSQSAAADGSRRISTCSGYSTTTDNGRHGSLTPSRRISGASSLSGYYSDERERSSQ---DSN------HKSQHWRYSYRRYEEEQRNEDAASSVTGTRHGMPRGSIGLL---PSSRKGSHDSTASGRSGSIISNAQWRKESVTIVEQVEPIHEQDDATSDRGDLAGTRRQNSLGMAEAPSQAVAKTTSLGSDGGTEEKFLAIPKKPITASSSAETLTDNT-LTAP----------------------PSPKSGGTPSPG--RNAPGPPQRRASHVDQL------VSVQSPSSMEDNEVDRPETTPLKSALKKCSISSITS---------GPTPKSRGRSTSTFSSASYSYQVNNDDDGSDLIAARGCSSQPGSHHPSFSSPTGSRIIFGGLNLKDLTTSMKSPTKEHKKKKGIFNRHIASATSNDSSNDHSSSPISSPQHSKMGVVVTSSRQAHRRSSSATAAVAFAVATAGSGNPPDPTNKDVFKEAA----------KRKMSLTSSVSTMRVLSVLRHWVTKHAQ 1289
            +CFAI YRRENQR+YEL+A T ++C+ WID I+ ASFNKL LQ EELEQKHLHLLQ+VESEKTAKWQYTQ C+EL  EIK +R EL  LKKE R                                L P+I  S  S   G +   E   ++ KIKKVQSFFRGWLCRRRWKQIVE+YI+SPHAESMRKRN LVF+MVEAEEEY EQ+EIL+SCFLRPFKMAASSK PPC+HED+NSIFLNSETVLFLHQIFLKGLT+RMESWPTLVLG    DLFD+LLPMLSIYQEYVRNHHY LQVLTECKQ   F  LL+RLE K AC GRSLE FLTYPMHQIPRYIITLHELLAHTP DHVE+KSL++AR QLEDLSRQMHDEVSETEN+RKNLA+ERMI+EGCDILLDVNQ+FVRQG LVQIL  P G++S            ++EAVRQCFLFSNHL++TTR     GRL+L+  +GKIPL+D  L+EDP E+   D+ E       SG S+S   S  GNT  + ++ DFK++LD    G  +T+HL+APT QEKA+WI+DI+QCMD VHFN +F+ +M + SS T+PQ +K+DP LFKDDVDIRFS+T N+CKIPQIR ATPERLLQRLTDLRFL+IDFLNTFLLTYRVFT+G+TVLEALK VF+   P     P  S  S++ L  T +EG                            T TD  R  S    RR SGASS+SGY S+  ER      +SN      +   HWR   +R EEEQ                   S+GL+   PS  K S    +S  S +  S+A  RK S+ +          D+ T + G                                     L IP K I  SSS+ETLT+ T L+AP                      PSP+ GGT S G       P     S  D++      V++ S  S +    ++  +TP      K     I             TP S G+S +T  S    YQ                      HH    +   S  +  G+    ++T M +       ++ I    + S++  +  +D  +S       SK GVVVTS R++HRRSS++TAA AFA+AT+ S NPPD   K ++K +           +RK S+ S+ +TMRVL+VLRHWV+KHAQ
Sbjct:   93 HCFAITYRRENQRQYELKASTETDCKQWIDAIREASFNKLLLQKEELEQKHLHLLQIVESEKTAKWQYTQQCEELASEIKKLRAELCTLKKELRPSVAQGFTTRPGFQRTLSQGTGSLASGLVHYGLYPEIQGSLVSCSLGLRGITEGSVEMQKIKKVQSFFRGWLCRRRWKQIVEQYIKSPHAESMRKRNSLVFQMVEAEEEYTEQMEILVSCFLRPFKMAASSKKPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRMESWPTLVLG----DLFDILLPMLSIYQEYVRNHHYSLQVLTECKQSSPFVALLNRLENKTACHGRSLEMFLTYPMHQIPRYIITLHELLAHTPHDHVERKSLQNARQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGALVQILDKPRGARSRLSATFGGKGSSDKEAVRQCFLFSNHLLLTTRQSDDDGRLNLIPHIGKIPLSDAVLIEDPCEQGVTDDDEFSVCSMSSGISESCG-SASGNTSSQLQNRDFKIVLDVKSGGPLVTVHLVAPTIQEKAAWISDISQCMDNVHFNDIFHCSMSDTSSVTMPQSIKNDPKLFKDDVDIRFSRTLNSCKIPQIRSATPERLLQRLTDLRFLSIDFLNTFLLTYRVFTDGVTVLEALKKVFYETEPPDAQMPCGSLVSLDVLGGTMSEG---------------------------QTHTDERRRSS-VSPRRTSGASSVSGYGSEISERDRHYMYESNTGAHTLNSKTHWRGGCKRLEEEQ-------------------SLGLITEAPSIMKHSPTLQSSNPSVTTQSSATSRKISIRV----------DNNTENDG-----------------------------------CHLTIP-KVIAGSSSSETLTEATVLSAPSSPSNLSTVTLIGSTGSGSGSDPSPQEGGTISRGISEETDTPVATSTSSKDEVQFTYADVTLTSGKSTDSETTEKTPSTPATPQTPKIKDHEIEKETRKEWTEEEKTPVSIGQSQTT--SIQTKYQ---------------------QHHMEHRASVASAPV-TGMRSGSISTVMGN---HWATRRSIQESDLCSSSQGNFQHDQQAS-------SKAGVVVTSFRRSHRRSSTSTAATAFAIATSASSNPPD---KVLWKNSVSDGSNRNDKNRRKESVMSTAATMRVLNVLRHWVSKHAQ 1184          

HSP 2 Score: 386.341 bits (991), Expect = 1.843e-106
Identity = 185/278 (66.55%), Postives = 220/278 (79.14%), Query Frame = 0

Query: 1534 EEPEESKGKKSGTLKPSASSGALNGLRQVPSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSS 1811
            EEPE +K      L P            V SKE+I +LSALEIAEQM +LD +I  +I S EFLGQAW K DK  R+PHI++MTKRFNE+S+LVVS+I+ + ++  RV  IEKW AVADI R LHN+NGVLQICAAFTNS+V+RLK TW +VSK++KQTIEKLQ +VSSDGRFRNLRDALHR DPPCIPYLG+YL+DL+FIEEGTPNFT+  LLNF+KMRMIAHVIREIR FQQTPYKIE + KVTNY+LD    L+++ LY+ SL +EPR SRLSSS
Sbjct: 1227 EEPESNKVDLKKLLTPPT----------VASKESIETLSALEIAEQMTYLDHQIFISITSEEFLGQAWMKTDKATRAPHILLMTKRFNEVSQLVVSEIIRRSNMSARVAAIEKWAAVADISRVLHNYNGVLQICAAFTNSSVYRLKKTWEKVSKTTKQTIEKLQNIVSSDGRFRNLRDALHRCDPPCIPYLGLYLTDLSFIEEGTPNFTEEGLLNFSKMRMIAHVIREIRHFQQTPYKIELITKVTNYLLDSSLLLNEKDLYRMSLEIEPRASRLSSS 1494          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. nr
Match: gi|646699733|gb|KDR10231.1| (Ras-specific guanine nucleotide-releasing factor 2, partial [Zootermopsis nevadensis])

HSP 1 Score: 944.11 bits (2439), Expect = 0.000e+0
Identity = 481/752 (63.96%), Postives = 572/752 (76.06%), Query Frame = 0

Query:  171 YCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGRLLPQISKSSSSQDG-------------DKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSL----SDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNPDQGSSPSSSNGSMENLTHAEGSIQVNLLHPSQSAAADGSRRISTCSGYSTTTDNGRHGSLTPSRRISGASSLSGYYSD--ERERS-SQDSNHKSQ-HWRYSYRRYEEEQR 901
            +CFAI YRRENQR+YELRA T S+C+ WI+ I+ ASFNKL LQ EELEQKHLHLLQ+VESEKTAKWQYTQ C+EL  EIK +R EL ALKKE R +   + S+S   G               +  +E  ++ KIKKVQSFFRGWLCRRRWKQIVE+YI+SPHAESMRKRN LVFRMVEAEEEY+EQLE+L+SCFLRPFKMAASSK PPC+HED+NSIFLNSETVLFLHQIFLKGLT+RMESWPTLVLG    DLFDMLLPMLSIYQEYVRNHHY LQVLTECKQ   F  LL RLE KPAC+GRSLETFLTYPMHQIPRYIITLHELLAHTP DHVE+KSL++AR QLEDLSRQMHDEVSETEN+RKNLA+ERMI+EGCDILLDVNQ+FVRQG+L+QI P+     +R RL    + E+E++RQCFLFSNHLI+ TRT  GRLHLV  +GKIPLAD  L+EDPS+    D+ ES   S  S S     +  G+  + ++  DFK+++D      Q+ +HL++PT QEKA+WI+DI+QCMD VHFN L +++M + SS T+PQ +++DP L+KDDVDIRFS T N+CK+PQIRYATPERLL+RLTDLRFL+IDFLNTFLLTYRVFT+G+T+LEALK VF+N D   + +S+ GS+ +L         N LH                       D+ R  S    RR SGASS+SGY S+  +R+RS S DS   S+ HWR  +R++EEE R
Sbjct:    1 HCFAISYRRENQRQYELRAITESDCKTWIEAIREASFNKLLLQKEELEQKHLHLLQIVESEKTAKWQYTQQCEELASEIKKLRAELCALKKEWRPIASYNTSTSLNSGLSFQPSAGGTGASTSTNCQETLELRKIKKVQSFFRGWLCRRRWKQIVEQYIKSPHAESMRKRNSLVFRMVEAEEEYMEQLEVLVSCFLRPFKMAASSKKPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRMESWPTLVLG----DLFDMLLPMLSIYQEYVRNHHYSLQVLTECKQSTPFAVLLARLENKPACQGRSLETFLTYPMHQIPRYIITLHELLAHTPHDHVERKSLQNARQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGSLIQI-PSDKPRTSRSRLT-SFKSEKESLRQCFLFSNHLILATRTSGGRLHLVPDIGKIPLADALLIEDPSDHGVNDDDESSVCSMSSQSSGISETSSGSCHFHNR--DFKIVIDMKTGCPQMVVHLVSPTIQEKAAWISDISQCMDNVHFNDLLHSSMSDTSSVTMPQSIRNDPKLYKDDVDIRFSHTLNSCKVPQIRYATPERLLERLTDLRFLSIDFLNTFLLTYRVFTDGVTILEALKKVFYNADPPEAQTSTAGSIMSLDVLCVREDHNTLH---------------------QYDSERRRSSASPRRTSGASSVSGYGSEMSDRDRSHSYDSQAFSKGHWRCGFRKFEEESR 723          

HSP 2 Score: 374.785 bits (961), Expect = 1.701e-103
Identity = 179/249 (71.89%), Postives = 214/249 (85.94%), Query Frame = 0

Query: 1563 PSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSS 1811
            PSKENI +LSALEIAEQM ++D +I  +I S EFLGQAW K DK  ++PHI++MTKRFNE+S+LVVS+IV + ++  R+  IEKW AVADI RCLHNFNGVLQ+CAAFTNS+VFRLK TW +VSK++KQTIEKLQ +VSSDGRFRNLRD+L R DPPCIPYLGMYL+DL+FIEEGTPNFT++ LLNF+KMRMIAHVI+EIR FQQTPYKI+ + KVTNY+LD    LDD+ LY+ SL +EPRTSRLSSS
Sbjct: 1073 PSKENIETLSALEIAEQMTYVDHQIFVSISSEEFLGQAWMKTDKATKAPHIILMTKRFNEVSQLVVSEIVRRSNMASRIAAIEKWAAVADISRCLHNFNGVLQVCAAFTNSSVFRLKKTWEKVSKTTKQTIEKLQKIVSSDGRFRNLRDSLRRCDPPCIPYLGMYLTDLSFIEEGTPNFTEDGLLNFSKMRMIAHVIQEIRHFQQTPYKIDLITKVTNYLLDTSLLLDDDELYRMSLEIEPRTSRLSSS 1321          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. nr
Match: gi|1339071954|ref|XP_023723186.1| (ras-specific guanine nucleotide-releasing factor 1-like isoform X2 [Cryptotermes secundus])

HSP 1 Score: 943.34 bits (2437), Expect = 0.000e+0
Identity = 493/753 (65.47%), Postives = 581/753 (77.16%), Query Frame = 0

Query:  171 YCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGRLLPQISKSSSSQDGDKSED-------------EEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSL----SDMGNTRYEHKSIDFKLILDDVKSGN-QITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNPDQGSSPSSSNGSMENLTHAEGSIQVNLLHPSQSAAADGSRRISTCSGYSTTTDNGRHGSLTPSRRISGASSLSGYYSD--ERERS-SQDSNHKSQ-HWRYSYRRYEEEQR 901
            +CFAI YRRENQR+YELRA T S+C+ WI+ I+ ASFNKL LQ EELEQKHLHLLQ+VESEKTAKWQYTQ C+EL  EIK +R EL ALKKE R +P  S ++S   G  S+              +E  ++ KIKKVQSFFRGWLCRRRWKQIVE+YI+SPHAESMRKRN LVFRMVEAEEEY+EQ+E+L+SCFLRPFKMAASSK PPC+HED+NSIFLNSETVLFLHQIFLKGLT+RMESWPTLVLG    DLFDMLLPMLSIYQEYVRNHHY LQVLTECKQ   F  LL RLE KPAC+GRSLE FLTYPMHQIPRYIITLHELLAHTP DHVE+KSL++AR QLEDLSRQMHDEVSETEN+RKNLA+ERMI+EGCDILLDVNQ+FVRQG+L+QI P+     +R RL    + E+EA+RQCFLFSNHLI+ TRT  GRLHLV  +GKIPLAD  L+EDPS+    D+ ES   S  S S     +  G+    ++  DFK+++ DVK+G  Q+ +HL+APT QEKA+WI+DI+QCMD VHFN L +++M + SS T+PQ +K+DP LFKDDVDIRFS+T N+CK+PQIRYATPERLL+RLTDLRFL+IDFLNTFLLTYRVFT+G+TVLEALK VF+N D   + +S+ GS+ +L      + V   H             ST   Y    D+ R  S    RR SGASS+SGY S+  +R+RS S DS   S+ HWR S+RR+EEE R
Sbjct:  100 HCFAISYRRENQRQYELRASTESDCKTWIEAIREASFNKLLLQKEELEQKHLHLLQIVESEKTAKWQYTQQCEELASEIKKLRAELCALKKEWRPVPSYSTNASLNSGLSSQPSAGGSGASTATSCQETMELRKIKKVQSFFRGWLCRRRWKQIVEQYIKSPHAESMRKRNSLVFRMVEAEEEYMEQMEVLVSCFLRPFKMAASSKKPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRMESWPTLVLG----DLFDMLLPMLSIYQEYVRNHHYSLQVLTECKQSALFAALLARLENKPACQGRSLEIFLTYPMHQIPRYIITLHELLAHTPHDHVERKSLQNARQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGSLIQI-PSDKPRTSRSRLT-NFKSEKEALRQCFLFSNHLILATRTSGGRLHLVPEIGKIPLADALLIEDPSDHGVNDDDESSVCSMSSQSSGISETSSGSCHLHNR--DFKIVI-DVKTGFPQVVVHLVAPTMQEKAAWISDISQCMDNVHFNDLLHSSMSDTSSVTMPQSIKNDPRLFKDDVDIRFSRTLNSCKVPQIRYATPERLLERLTDLRFLSIDFLNTFLLTYRVFTDGVTVLEALKKVFYNADPPEAQTSTAGSIMSL----DVLGVREDH-------------STLHLY----DSERRRSSASPRRTSGASSVSGYGSEMSDRDRSHSYDSQAFSKGHWRCSFRRFEEESR 822          

HSP 2 Score: 383.259 bits (983), Expect = 2.229e-105
Identity = 190/278 (68.35%), Postives = 225/278 (80.94%), Query Frame = 0

Query: 1534 EEPEESKGKKSGTLKPSASSGALNGLRQVPSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSS 1811
            EEPE SK      L P  +          PSKENI +LSALEIAEQM ++D +I  +I S EFLGQAW K DK  ++PHI++MT+RFNE+S+LVVS+IV + ++  R+  IEKW AVADI RCLHNFNGVLQ+CAAFTNS+VFRLK TW +VSK++KQTIEKLQ +VSSDGRFRNLRDALHR DPPCIPYLGMYL+DL+FIEEGTPNFT++ LLNF+KMRMIAHVIREIR FQQTPYKI+ + KVTNY+LDP   LDD+ LY+ SL +EPRTSRLSSS
Sbjct: 1221 EEPESSKVDLDLLLAPPMT----------PSKENIETLSALEIAEQMTYVDHQIFISISSEEFLGQAWMKTDKATKAPHIILMTRRFNEVSQLVVSEIVRRSNMASRIAAIEKWAAVADISRCLHNFNGVLQVCAAFTNSSVFRLKKTWEKVSKTTKQTIEKLQNIVSSDGRFRNLRDALHRCDPPCIPYLGMYLTDLSFIEEGTPNFTEDGLLNFSKMRMIAHVIREIRLFQQTPYKIDLITKVTNYLLDPSLLLDDDELYRMSLEIEPRTSRLSSS 1488          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. nr
Match: gi|1228009667|ref|XP_021936754.1| (ras-specific guanine nucleotide-releasing factor 2-like isoform X2 [Zootermopsis nevadensis])

HSP 1 Score: 942.954 bits (2436), Expect = 0.000e+0
Identity = 481/752 (63.96%), Postives = 572/752 (76.06%), Query Frame = 0

Query:  171 YCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGRLLPQISKSSSSQDG-------------DKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSL----SDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNPDQGSSPSSSNGSMENLTHAEGSIQVNLLHPSQSAAADGSRRISTCSGYSTTTDNGRHGSLTPSRRISGASSLSGYYSD--ERERS-SQDSNHKSQ-HWRYSYRRYEEEQR 901
            +CFAI YRRENQR+YELRA T S+C+ WI+ I+ ASFNKL LQ EELEQKHLHLLQ+VESEKTAKWQYTQ C+EL  EIK +R EL ALKKE R +   + S+S   G               +  +E  ++ KIKKVQSFFRGWLCRRRWKQIVE+YI+SPHAESMRKRN LVFRMVEAEEEY+EQLE+L+SCFLRPFKMAASSK PPC+HED+NSIFLNSETVLFLHQIFLKGLT+RMESWPTLVLG    DLFDMLLPMLSIYQEYVRNHHY LQVLTECKQ   F  LL RLE KPAC+GRSLETFLTYPMHQIPRYIITLHELLAHTP DHVE+KSL++AR QLEDLSRQMHDEVSETEN+RKNLA+ERMI+EGCDILLDVNQ+FVRQG+L+QI P+     +R RL    + E+E++RQCFLFSNHLI+ TRT  GRLHLV  +GKIPLAD  L+EDPS+    D+ ES   S  S S     +  G+  + ++  DFK+++D      Q+ +HL++PT QEKA+WI+DI+QCMD VHFN L +++M + SS T+PQ +++DP L+KDDVDIRFS T N+CK+PQIRYATPERLL+RLTDLRFL+IDFLNTFLLTYRVFT+G+T+LEALK VF+N D   + +S+ GS+ +L         N LH                       D+ R  S    RR SGASS+SGY S+  +R+RS S DS   S+ HWR  +R++EEE R
Sbjct:   95 HCFAISYRRENQRQYELRAITESDCKTWIEAIREASFNKLLLQKEELEQKHLHLLQIVESEKTAKWQYTQQCEELASEIKKLRAELCALKKEWRPIASYNTSTSLNSGLSFQPSAGGTGASTSTNCQETLELRKIKKVQSFFRGWLCRRRWKQIVEQYIKSPHAESMRKRNSLVFRMVEAEEEYMEQLEVLVSCFLRPFKMAASSKKPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRMESWPTLVLG----DLFDMLLPMLSIYQEYVRNHHYSLQVLTECKQSTPFAVLLARLENKPACQGRSLETFLTYPMHQIPRYIITLHELLAHTPHDHVERKSLQNARQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGSLIQI-PSDKPRTSRSRLT-SFKSEKESLRQCFLFSNHLILATRTSGGRLHLVPDIGKIPLADALLIEDPSDHGVNDDDESSVCSMSSQSSGISETSSGSCHFHNR--DFKIVIDMKTGCPQMVVHLVSPTIQEKAAWISDISQCMDNVHFNDLLHSSMSDTSSVTMPQSIRNDPKLYKDDVDIRFSHTLNSCKVPQIRYATPERLLERLTDLRFLSIDFLNTFLLTYRVFTDGVTILEALKKVFYNADPPEAQTSTAGSIMSLDVLCVREDHNTLH---------------------QYDSERRRSSASPRRTSGASSVSGYGSEMSDRDRSHSYDSQAFSKGHWRCGFRKFEEESR 817          

HSP 2 Score: 374.785 bits (961), Expect = 1.049e-102
Identity = 179/249 (71.89%), Postives = 214/249 (85.94%), Query Frame = 0

Query: 1563 PSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVIREIRQFQQTPYKIEQVPKVTNYILDPERQLDDEMLYQQSLIVEPRTSRLSSS 1811
            PSKENI +LSALEIAEQM ++D +I  +I S EFLGQAW K DK  ++PHI++MTKRFNE+S+LVVS+IV + ++  R+  IEKW AVADI RCLHNFNGVLQ+CAAFTNS+VFRLK TW +VSK++KQTIEKLQ +VSSDGRFRNLRD+L R DPPCIPYLGMYL+DL+FIEEGTPNFT++ LLNF+KMRMIAHVI+EIR FQQTPYKI+ + KVTNY+LD    LDD+ LY+ SL +EPRTSRLSSS
Sbjct: 1232 PSKENIETLSALEIAEQMTYVDHQIFVSISSEEFLGQAWMKTDKATKAPHIILMTKRFNEVSQLVVSEIVRRSNMASRIAAIEKWAAVADISRCLHNFNGVLQVCAAFTNSSVFRLKKTWEKVSKTTKQTIEKLQKIVSSDGRFRNLRDSLRRCDPPCIPYLGMYLTDLSFIEEGTPNFTEDGLLNFSKMRMIAHVIQEIRHFQQTPYKIDLITKVTNYLLDTSLLLDDDELYRMSLEIEPRTSRLSSS 1480          

HSP 3 Score: 71.2478 bits (173), Expect = 2.109e-8
Identity = 111/330 (33.64%), Postives = 155/330 (46.97%), Query Frame = 0

Query: 1012 LAIPKKPITASSSAETLTDNT-LTAPPSPKSGGTPS--PGRNAPGPPQ-RRASHVD-------QLVSVQSPSSMEDNEVDRP--------ETTP------LKSALKKCSISSITS----GPTPKSRGRSTSTFSSASYSYQVNNDDDG--SDLIAARGCSSQPGSHHPSFSSPTGSRIIFGGLNLKDLTTSMKSPTKEHKKKKGIFNRHIASATSNDSSNDHSSSPISSPQHS-KMGVVVTSSRQAHRRSSSATAAVAFAVATAGSGNPPD---PTNKDV-----------------FKEAAKRKMSLTSSVSTMRVLSVLRHWVTKHAQ 1289
            L IPK  IT SSS+ETLTD T ++AP SP +  T +     N+   PQ    SH++        LV+ Q      + EV  P        +T P       ++  K+ S+  + S      T   R  S        Y  +    DD   SD + A G S QP +H     + T SR     +   ++  S  +  K  +  +G       S+ S  +S  H S     PQ+S K GVV+TS RQ+HRRSS+++AA AFA+AT+ S NP D   PT + V                  ++  +RK S+ S+ +TMRVLSVLRHWV+KHAQ
Sbjct:  857 LTIPKT-ITVSSSSETLTDITVISAPSSPSNLSTVTLVGSNNSEMSPQDNETSHMEVEMESETDLVTKQQKYIRAEPEVMSPVIRESSEEQTKPSSMSVQFRNDEKRASVPIVESERRLSRTQVKRKGSEGQARREGYEEEKGKPDDFHVSDELCAEGLS-QPVTHE--HKAGTSSR--SASITTTNVIQSYYASRKSIQDLEGG-----GSSMSQRTSFQHDS-----PQNSSKAGVVITSFRQSHRRSSTSSAAAAFAIATSASSNPRDHTIPTKQPVGAGGRCSVGDASMRVGSCRDRNRRKESVMSTAATMRVLSVLRHWVSKHAQ 1170          

BLAST of ras-specific guanine nucleotide-releasing factor 1-like vs. nr
Match: gi|1228009671|ref|XP_021936756.1| (ras-specific guanine nucleotide-releasing factor 2-like isoform X4 [Zootermopsis nevadensis])

HSP 1 Score: 942.954 bits (2436), Expect = 0.000e+0
Identity = 481/752 (63.96%), Postives = 572/752 (76.06%), Query Frame = 0

Query:  171 YCFAIMYRRENQRRYELRADTISECQAWIDGIKMASFNKLHLQMEELEQKHLHLLQVVESEKTAKWQYTQHCDELKDEIKTIRVELFALKKEGRLLPQISKSSSSQDG-------------DKSEDEEDPDIAKIKKVQSFFRGWLCRRRWKQIVEEYIRSPHAESMRKRNCLVFRMVEAEEEYVEQLEILISCFLRPFKMAASSKNPPCNHEDINSIFLNSETVLFLHQIFLKGLTARMESWPTLVLGTSLGDLFDMLLPMLSIYQEYVRNHHYCLQVLTECKQIPQFGTLLHRLEKKPACKGRSLETFLTYPMHQIPRYIITLHELLAHTPPDHVEKKSLEHARIQLEDLSRQMHDEVSETENIRKNLAIERMIIEGCDILLDVNQMFVRQGTLVQILPAGSKSGARGRLAIGREKEREAVRQCFLFSNHLIVTTRTQAGRLHLVDGVGKIPLADVTLVEDPSEEERMDEPESPSGSSDSSSL----SDMGNTRYEHKSIDFKLILDDVKSGNQITIHLIAPTHQEKASWITDITQCMDTVHFNGLFYNTMPNASSSTIPQCVKSDPNLFKDDVDIRFSKTFNTCKIPQIRYATPERLLQRLTDLRFLNIDFLNTFLLTYRVFTNGITVLEALKTVFFNPDQGSSPSSSNGSMENLTHAEGSIQVNLLHPSQSAAADGSRRISTCSGYSTTTDNGRHGSLTPSRRISGASSLSGYYSD--ERERS-SQDSNHKSQ-HWRYSYRRYEEEQR 901
            +CFAI YRRENQR+YELRA T S+C+ WI+ I+ ASFNKL LQ EELEQKHLHLLQ+VESEKTAKWQYTQ C+EL  EIK +R EL ALKKE R +   + S+S   G               +  +E  ++ KIKKVQSFFRGWLCRRRWKQIVE+YI+SPHAESMRKRN LVFRMVEAEEEY+EQLE+L+SCFLRPFKMAASSK PPC+HED+NSIFLNSETVLFLHQIFLKGLT+RMESWPTLVLG    DLFDMLLPMLSIYQEYVRNHHY LQVLTECKQ   F  LL RLE KPAC+GRSLETFLTYPMHQIPRYIITLHELLAHTP DHVE+KSL++AR QLEDLSRQMHDEVSETEN+RKNLA+ERMI+EGCDILLDVNQ+FVRQG+L+QI P+     +R RL    + E+E++RQCFLFSNHLI+ TRT  GRLHLV  +GKIPLAD  L+EDPS+    D+ ES   S  S S     +  G+  + ++  DFK+++D      Q+ +HL++PT QEKA+WI+DI+QCMD VHFN L +++M + SS T+PQ +++DP L+KDDVDIRFS T N+CK+PQIRYATPERLL+RLTDLRFL+IDFLNTFLLTYRVFT+G+T+LEALK VF+N D   + +S+ GS+ +L         N LH                       D+ R  S    RR SGASS+SGY S+  +R+RS S DS   S+ HWR  +R++EEE R
Sbjct:   95 HCFAISYRRENQRQYELRAITESDCKTWIEAIREASFNKLLLQKEELEQKHLHLLQIVESEKTAKWQYTQQCEELASEIKKLRAELCALKKEWRPIASYNTSTSLNSGLSFQPSAGGTGASTSTNCQETLELRKIKKVQSFFRGWLCRRRWKQIVEQYIKSPHAESMRKRNSLVFRMVEAEEEYMEQLEVLVSCFLRPFKMAASSKKPPCSHEDVNSIFLNSETVLFLHQIFLKGLTSRMESWPTLVLG----DLFDMLLPMLSIYQEYVRNHHYSLQVLTECKQSTPFAVLLARLENKPACQGRSLETFLTYPMHQIPRYIITLHELLAHTPHDHVERKSLQNARQQLEDLSRQMHDEVSETENLRKNLAVERMIVEGCDILLDVNQVFVRQGSLIQI-PSDKPRTSRSRLT-SFKSEKESLRQCFLFSNHLILATRTSGGRLHLVPDIGKIPLADALLIEDPSDHGVNDDDESSVCSMSSQSSGISETSSGSCHFHNR--DFKIVIDMKTGCPQMVVHLVSPTIQEKAAWISDISQCMDNVHFNDLLHSSMSDTSSVTMPQSIRNDPKLYKDDVDIRFSHTLNSCKVPQIRYATPERLLERLTDLRFLSIDFLNTFLLTYRVFTDGVTILEALKKVFYNADPPEAQTSTAGSIMSLDVLCVREDHNTLH---------------------QYDSERRRSSASPRRTSGASSVSGYGSEMSDRDRSHSYDSQAFSKGHWRCGFRKFEEESR 817          

HSP 2 Score: 313.538 bits (802), Expect = 4.216e-83
Identity = 140/197 (71.07%), Postives = 169/197 (85.79%), Query Frame = 0

Query: 1563 PSKENINSLSALEIAEQMAHLDQKILFTIQSSEFLGQAWTKPDKDVRSPHIVIMTKRFNEMSRLVVSDIVTQPDVGIRVQVIEKWTAVADICRCLHNFNGVLQICAAFTNSAVFRLKNTWNRVSKSSKQTIEKLQALVSSDGRFRNLRDALHRTDPPCIPYLGMYLSDLTFIEEGTPNFTDNHLLNFAKMRMIAHVI 1759
            PSKENI +LSALEIAEQM ++D +I  +I S EFLGQAW K DK  ++PHI++MTKRFNE+S+LVVS+IV + ++  R+  IEKW AVADI RCLHNFNGVLQ+CAAFTNS+VFRLK TW +VSK++KQTIEKLQ +VSSDGRFRNLRD+L R DPPCIPYLGMYL+DL+FIEEGTPNFT++ LLNF+KMRM   V+
Sbjct: 1239 PSKENIETLSALEIAEQMTYVDHQIFVSISSEEFLGQAWMKTDKATKAPHIILMTKRFNEVSQLVVSEIVRRSNMASRIAAIEKWAAVADISRCLHNFNGVLQVCAAFTNSSVFRLKKTWEKVSKTTKQTIEKLQKIVSSDGRFRNLRDSLRRCDPPCIPYLGMYLTDLSFIEEGTPNFTEDGLLNFSKMRMCLSVV 1435