BLAST of gtp-binding protein vs. L. salmonis genes
Match: EMLSAG00000008145 (supercontig:LSalAtl2s:LSalAtl2s487:142410:144801:1 gene:EMLSAG00000008145 transcript:EMLSAT00000008145 description:"maker-LSalAtl2s487-augustus-gene-1.18")

HSP 1 Score: 444.506 bits (1142), Expect = 1.569e-148
Identity = 227/573 (39.62%), Postives = 359/573 (62.65%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPK-GDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEV---KPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNI-TNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAL 568
            +LE+ G + +     Q+LD + +ERERGIT+K+    + +++    Y +NLIDTPGHVDF+ EV+RS+ AC+G +L+VDA QG++AQT+SN F A   D  IIPV+NK+DLP A  EEVK Q++ L  ID   ++ ASAK GIGI DILN +I  VP P  G  D   +  + DS ++ ++G   L +V  G ++ G  +    T K Y    +G L   + P  +I  G +GY+   ++  K+  +GDT     + + + I+   +V   KPMVFAG YP++  ++ +L+  +EK+ LNDA++   PE   +LG G+R GFLG+LH+E+  +RL+ E+   V+ T PSV + +    + +   +   S  PD N I+   EP +   I+  +DY+     L    RG   +  Y+   RV++   +PL E++ +FF++LK  + G+ S DY+  GY+ + L K++IL+NG+P++ L+ I H+ K+ +  ++ CE LKEL+PR+QF + IQA +  +IIARE+V+A++KDVTAKCYGGD+SR+RKLL+ Q +GKK++++ G V++ ++  + L
Sbjct:   62 ILEYVGAIDKSVDNKQVLDKLRVERERGITVKAQTASIIYSKGNMEYLINLIDTPGHVDFASEVTRSLKACQGVVLLVDANQGVQAQTMSNFFAAFSEDKAIIPVLNKVDLPKADPEEVKKQMLKLFEIDPNTVIHASAKFGIGIVDILNRVISDVPPPPVGRSDHKFRCFLQDSWYDRFKGATNLVQVTEGVLSIGMTIASKMTDKSYSIKSLGLLTPDEFPVAKIYPGQMGYMACNMRSPKDAIIGDTFFDKSQGSGSDIEPVVDVIPCKPMVFAGFYPIDQNEYVKLKSAIEKVCLNDASVSVTPECCVSLGQGYRLGFLGILHLEVFSQRLDDEYGAQVVITSPSVPYKLKVTGQSDLVLILNTSNWPDKN-IEEYHEPMVRGIILAPSDYLRACYGLIQEYRGKEVDTEYIDDTRVKIDLSIPLNEILTEFFNELKLRTSGFGSFDYSDDGYKRTTLTKVEILINGEPMNELARICHISKSDEIARKTCETLKELMPRRQFLVTIQARLNGRIIARENVKALKKDVTAKCYGGDLSRQRKLLKNQAEGKKKLKESGKVKISKDTLIKL 633          

HSP 2 Score: 105.916 bits (263), Expect = 5.122e-24
Identity = 46/86 (53.49%), Postives = 70/86 (81.40%), Query Frame = 0

Query:  583 LASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVL 668
            +A K CE LKEL+PR+QF + IQA +  +IIARE+V+A++KDVTAKCYGGD+SR+RKLL+ Q +GKK++++ G V++ ++  + +L
Sbjct:  549 IARKTCETLKELMPRRQFLVTIQARLNGRIIARENVKALKKDVTAKCYGGDLSRQRKLLKNQAEGKKKLKESGKVKISKDTLIKLL 634          

BLAST of gtp-binding protein vs. L. salmonis genes
Match: EMLSAG00000005476 (supercontig:LSalAtl2s:LSalAtl2s294:457338:461054:1 gene:EMLSAG00000005476 transcript:EMLSAT00000005476 description:"augustus_masked-LSalAtl2s294-processed-gene-4.3")

HSP 1 Score: 90.1225 bits (222), Expect = 6.703e-19
Identity = 58/157 (36.94%), Postives = 87/157 (55.41%), Query Frame = 0

Query:    4 FTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPK 160
            FT +V +    + ++D MD ERERGITI S AI   + +    + +NLIDTPGHVDF+ EV RS+   +G +LI+D + G++AQT++    A  + L  I  INK+D   A + +  D +   +GI          KE     DIL+ ++D +   K
Sbjct:   75 FTPSVGEVHTGDTVMDYMDQERERGITITSAAITFPWNK----HQINLIDTPGHVDFTLEVERSLRVLDGGILILDGSAGVQAQTVTVCRQAKRYYLPKIVFINKMDKRNANLNKSLDSLELKLGIQPLLTQYPIYKE-----DILSGLVDLISMEK 222          

HSP 2 Score: 56.225 bits (134), Expect = 2.042e-8
Identity = 56/228 (24.56%), Postives = 102/228 (44.74%), Query Frame = 0

Query:  121 DQVVDLIGIDRED---IVLASAKEGIGIDDILNAIIDRVPAPKG--DPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYE--------ADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREF 335
            +  + LI ++  D   ++L S+ + IGI  +L+AI   +P PK   +      G++F  + +  +G     R+ +G +  GD V  +  GK  +        AD+  S+       + +  G+   +ISG    KE   GDT+T  K   D +I G     P+++  I P   +   +L   ++ L   D +  +        G     G +G LH+ I+++R+E E+
Sbjct:  286 EHALRLITLNNYDAVLVLLGSSYKNIGIQLLLDAITKYLPPPKALKNHSNNFLGMVFKIIHHPNKGALSFIRIYSGKLKVGDLVFNVNKGKSEKIQQLLIAFADDFRSV-------NEVSHGNXA-VISGF---KESVTGDTLTSNKNSIDQLI-GVPVPNPVMYCTIEPPSMSQQSQLELALKNLSREDPS--FRLSVDEVTGQTLLSG-MGELHLSIMRDRIESEY 498          

BLAST of gtp-binding protein vs. L. salmonis genes
Match: EMLSAG00000000297 (supercontig:LSalAtl2s:LSalAtl2s1044:24227:29290:-1 gene:EMLSAG00000000297 transcript:EMLSAT00000000297 description:"maker-LSalAtl2s1044-augustus-gene-0.34")

HSP 1 Score: 88.5817 bits (218), Expect = 2.336e-18
Identity = 55/158 (34.81%), Postives = 85/158 (53.80%), Query Frame = 0

Query:   48 TLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDI------VLASAKEGIGIDDILNAI--IDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGD 197
            T+  IDTPGH  F    +R + A + A+L+VDA +G+  QT+ +L +  E     I  INKID P A +   K+ + +L  ++ ED       +  SA +G  +D  LNAI  +  V   KGDP   ++G+I +S   +  G   +F V+ G++ KGD
Sbjct:  194 TITFIDTPGHAAFKSMRNRGVCATDIAILVVDACEGVLEQTLESLSMIKESGRPYIVAINKIDKPNADVNATKESLKELGKVNLEDFGGDVQXISMSALKGTNVDGFLNAIGALAEVLELKGDPTGQVRGVILESQMEAGIGRSTVFLVQKGTLKKGD 351          

BLAST of gtp-binding protein vs. L. salmonis genes
Match: EMLSAG00000011687 (supercontig:LSalAtl2s:LSalAtl2s812:148775:152867:1 gene:EMLSAG00000011687 transcript:EMLSAT00000011687 description:"maker-LSalAtl2s812-augustus-gene-1.59")

HSP 1 Score: 77.7962 bits (190), Expect = 4.242e-15
Identity = 55/159 (34.59%), Postives = 91/159 (57.23%), Query Frame = 0

Query:   18 LDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPL-QGLIFDSV 175
            +D M+LER RGITI+S A  + + +D   Y +N+IDTPGHVDF+ EV RS+   +GA+L++ A  G+++QT++       + +  I  INK+D  G    +V  Q+     + R  ++L   +  IG D  +  ++D +       + PL + +I+DSV
Sbjct:   63 MDSMELERARGITIQSAATFIRW-KD---YNVNIIDTPGHVDFTIEVERSLRVLDGAVLVLCAVGGVQSQTMTVNRQMKRYRVPSIAFINKLDRTGGDAFKVHSQLRS--KLHRNAVLL---QLPIGKDSHVKGVVDIIKDRAIYFEGPLGEEVIYDSV 212          

BLAST of gtp-binding protein vs. L. salmonis genes
Match: EMLSAG00000008108 (supercontig:LSalAtl2s:LSalAtl2s482:182113:187772:1 gene:EMLSAG00000008108 transcript:EMLSAT00000008108 description:"maker-LSalAtl2s482-snap-gene-1.29")

HSP 1 Score: 63.1586 bits (152), Expect = 1.777e-10
Identity = 38/109 (34.86%), Postives = 62/109 (56.88%), Query Frame = 0

Query:    2 LEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKID 110
            L  +GT+       + LD    E+  GIT+KS A+ +        + +NLID+PGHVDFS EV  ++   +GAL+++D  + ++ QT + L  A    ++ I V+NK+D
Sbjct:   48 LRLSGTL-------RYLDSRPDEQAXGITMKSSAVSLYHEN----HLINLIDSPGHVDFSSEVCXAVRLSDGALVLIDVVESVQPQTRAVLKQAWVEGIKPILVLNKMD 145          

BLAST of gtp-binding protein vs. L. salmonis genes
Match: EMLSAG00000010619 (supercontig:LSalAtl2s:LSalAtl2s705:336281:337543:-1 gene:EMLSAG00000010619 transcript:EMLSAT00000010619 description:"augustus_masked-LSalAtl2s705-processed-gene-3.6")

HSP 1 Score: 58.151 bits (139), Expect = 4.077e-9
Identity = 72/271 (26.57%), Postives = 114/271 (42.07%), Query Frame = 0

Query:   18 LDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLE-IIPVINKIDLPG-AMIEEVKDQVVDLI---GIDREDIVLASAKEGIGIDD------------ILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIK---------VAKEVKVGDTITHLKRQADTMIK 262
            +D+   ER RGITI  +   +++  +   Y     D PGH DF   +       +GA+L+V A  G   QT  +L L  +  +E I+  INK D+    MIE V+ +V +L+   G   +DI +         +D            +++A+   +P P+ D D P    I        RG  V  RV+ G +  G +V+F+      ++   G     K  ++      +G L  G+K         VAK   V   + H+K Q   M K
Sbjct:   75 IDNAPEERSRGITI--NVAHIEYATENRHYAHT--DCPGHADFIKNMITGANNMDGAILVVGATDGCMPQTREHLLLIKQLGVEHIVVFINKCDVADEEMIELVEMEVRELLSENGFPGDDIPVIKGSALSACEDKNASLGKEQIVALMSAVDSYIPNPERDLDLPFLLPIEHVHTIPGRGTVVTGRVERGKLKVGQEVEFLGFNTAIKSKVTGIEMFHKILEEANAGDQMGILAXGVKKXDVXRGMVVAKPNSV-KQVDHIKAQVYLMSK 340          

BLAST of gtp-binding protein vs. L. salmonis genes
Match: EMLSAG00000008753 (supercontig:LSalAtl2s:LSalAtl2s543:242325:249249:1 gene:EMLSAG00000008753 transcript:EMLSAT00000008753 description:"maker-LSalAtl2s543-augustus-gene-1.72")

HSP 1 Score: 58.5362 bits (140), Expect = 5.358e-9
Identity = 33/94 (35.11%), Postives = 54/94 (57.45%), Query Frame = 0

Query:   19 DDMDLERERGITIKSHAIQM--DFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKID 110
            D +  E+ERG++IK+  + +     R    Y +NL+DTPGHV+FS EV+ ++   +G ++ VDA+ G+   T      A++    +   INKID
Sbjct:  153 DTLFTEQERGVSIKAMPLSLVCQGIRGQKSYLVNLLDTPGHVNFSDEVTAAMRLSDGVVVFVDASXGVMLNTERLXKHAVQEQCAVTICINKID 246          

BLAST of gtp-binding protein vs. SwissProt
Match: gi|119371343|sp|Q11UD0.1|LEPA_CYTH3 (RecName: Full=Elongation factor 4; Short=EF-4; AltName: Full=Ribosomal back-translocase LepA)

HSP 1 Score: 903.279 bits (2333), Expect = 0.000e+0
Identity = 434/573 (75.74%), Postives = 503/573 (87.78%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKG-EKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFT TV+ R+MQ Q+LDDMDLERERGITIKSHAIQM++  +G  Y LNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNL+LALE+DLEIIPV+NKIDLPGAM EEVKDQVV+L+G DR+ I+ AS KEGIGI +IL AII R+P PKGDP APLQ LIFDS FNS+RGIEV+FR+ NG+I KGDKVKF+  GKEY ADEIG L+L+++P+  +  G+VGY+ISGIK AK+VKVGDTITH+ R  +  I+GF +VKPMVFAGIYPVET++FE+LR  MEKLQLNDA+L+WEPETS ALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSV+F     KG E   V+APS+MP+P  +  +EEP+I AQIIT AD+VGP+I+LCM KRG+I NQ YLTSDRVELTF++PLAE+VFDFFDKLKTIS+GYASLDY L G+RES ++KLDI+LNG+ VDALSAIVH DKAYDWGK+LCEKLK+L+P Q FEIA+QAAIG KIIARE+V+AMRK+V AKCYGGDISRKRKLLEKQKKGKKRMRQ+GNVE+PQ AFMA+ + D
Sbjct:   24 LLEFTSTVSNRQMQAQVLDDMDLERERGITIKSHAIQMNYIYEGQKYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLYLALENDLEIIPVLNKIDLPGAMPEEVKDQVVELLGCDRDSIIPASGKEGIGIFEILAAIIQRIPPPKGDPKAPLQALIFDSQFNSFRGIEVMFRIFNGTIKKGDKVKFINIGKEYYADEIGVLKLQQEPQLEMSAGNVGYIISGIKEAKDVKVGDTITHVSRPGEP-IQGFADVKPMVFAGIYPVETSEFEDLRAAMEKLQLNDASLVWEPETSIALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVQFKAYLTKGNEMVVVNAPSDMPEPTTLDYIEEPYIKAQIITKADFVGPVISLCMDKRGIITNQTYLTSDRVELTFELPLAEIVFDFFDKLKTISKGYASLDYELIGFRESNVIKLDIMLNGEKVDALSAIVHRDKAYDWGKRLCEKLKDLIPMQMFEIAVQAAIGQKIIARETVKAMRKNVLAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQAAFMAILKLD 595          

HSP 2 Score: 149.828 bits (377), Expect = 5.564e-37
Identity = 69/86 (80.23%), Postives = 81/86 (94.19%), Query Frame = 0

Query:  586 KLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            +LCEKLK+L+P Q FEIA+QAAIG KIIARE+V+AMRK+V AKCYGGDISRKRKLLEKQKKGKKRMRQ+GNVE+PQ AFMA+LK++
Sbjct:  510 RLCEKLKDLIPMQMFEIAVQAAIGQKIIARETVKAMRKNVLAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQAAFMAILKLD 595          

BLAST of gtp-binding protein vs. SwissProt
Match: gi|238692313|sp|B3ESU2.1|LEPA_AMOA5 (RecName: Full=Elongation factor 4; Short=EF-4; AltName: Full=Ribosomal back-translocase LepA)

HSP 1 Score: 869.766 bits (2246), Expect = 0.000e+0
Identity = 416/572 (72.73%), Postives = 495/572 (86.54%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLE TGT++ +  Q+QLLD+MDLERERGITIKSHAIQM +T  GT Y LNLIDTPGHVDFSYEVSRSIAACEGALL++DA+QGIEAQTISNL+LALEHDL IIPV+NKIDLPGAM EEVKDQ+VDL+G DREDI+ ASAK+GIGI+DIL AII+R+  P G  DAPLQ +IFDSV+NS++G+EV FR+ NG+I +GD+VKF+ TGK YEADEIG LRL + P++ +  G VGYLISGIK A+EVKVGDTITH+       IKGFE+VKPMV+AGIYPVE+ ++EELR  +EKLQLNDA+L+W  E+SAALG GFRCGFLGMLHMEIVQERLEREFDMTVITTVPSV+F++ + +G+   V+APSE+PDPNLI  ++EPFI AQIIT  +++G II LC+ KRG ++NQ YLT +RVELTF +PLAEVVFDFFDKLKTIS+GYASLDY L G+  SKLVKLDILLN + +DALSAIVH DKAY+ GK+LC KLKELLP+Q FEI IQAAIG+K+IARE+++AMRK+V AKCYGGDISRKRKLLEKQKKGKKRMRQIG+VEVPQEAF+A+ + +
Sbjct:   24 LLEATGTISGKASQDQLLDNMDLERERGITIKSHAIQMRYTFKGTDYVLNLIDTPGHVDFSYEVSRSIAACEGALLVIDASQGIEAQTISNLYLALEHDLTIIPVLNKIDLPGAMPEEVKDQIVDLLGCDREDIIPASAKQGIGIEDILQAIIERISPPTGLVDAPLQAMIFDSVYNSFKGVEVYFRIFNGTIKQGDQVKFVNTGKSYEADEIGVLRLGQIPQESLSAGYVGYLISGIKNAREVKVGDTITHVNNPCKEAIKGFEDVKPMVYAGIYPVESNEYEELRTAIEKLQLNDASLVWTHESSAALGIGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVQFHVIDKQGDLHEVNAPSELPDPNLIDEIQEPFIRAQIITKPEFIGNIIKLCVDKRGALQNQTYLTPERVELTFKLPLAEVVFDFFDKLKTISKGYASLDYELLGFEASKLVKLDILLNEEKIDALSAIVHRDKAYELGKKLCAKLKELLPKQMFEIPIQAAIGTKVIARETIKAMRKNVIAKCYGGDISRKRKLLEKQKKGKKRMRQIGSVEVPQEAFLAVLKLE 595          

HSP 2 Score: 152.14 bits (383), Expect = 9.294e-38
Identity = 72/88 (81.82%), Postives = 82/88 (93.18%), Query Frame = 0

Query:  583 LASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKI 670
            L  KLC KLKELLP+Q FEI IQAAIG+K+IARE+++AMRK+V AKCYGGDISRKRKLLEKQKKGKKRMRQIG+VEVPQEAF+AVLK+
Sbjct:  507 LGKKLCAKLKELLPKQMFEIPIQAAIGTKVIARETIKAMRKNVIAKCYGGDISRKRKLLEKQKKGKKRMRQIGSVEVPQEAFLAVLKL 594          

BLAST of gtp-binding protein vs. SwissProt
Match: gi|166223199|sp|A0M6M2.1|LEPA_GRAFK (RecName: Full=Elongation factor 4; Short=EF-4; AltName: Full=Ribosomal back-translocase LepA)

HSP 1 Score: 852.047 bits (2200), Expect = 0.000e+0
Identity = 406/572 (70.98%), Postives = 489/572 (85.49%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKG--EKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALAR 570
            LL+FTG VT+RE Q QLLD MDLERERGITIKSHAIQMD+  +G  + LNLIDTPGHVDFSYEVSRSIAACEGALL+VDAAQ I+AQTISNL+LALE+DLEIIPV+NK+DLP A  EEV D +VDL+G D  +++ ASAK G+GI +IL+AII+RVPAP G  DAPL+ LIFDSV+N +RG+E  FRV NGSI KG+K+KF+ATGK Y+ADE+G+LRLK+ P+  I+ GDVGYLI+GIK A+EVKVGDTIT         + GFE+VKPMVFAGIYPV+T D+E+LR  MEKLQLNDA+L++ PE+SAALGFGFRCGFLGMLH+EI+QERLEREFDMTVITTVP+V ++   NK   +   V+ PS++P+P+ +  +EEPFI A IIT ADYVG ++ LC+ KRG I NQ YLT++RVELTFDMPLAE+VFDF+D+LKT+SRGYAS DYT  G RESKLVK+D+LLN + VDALSA++HVD AYD GK++CEKLKEL+PRQQF+I IQAAIG+KIIARE+V+A+RKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQ+GNVE+PQEAFMA+ +
Sbjct:   24 LLDFTGAVTEREKQEQLLDSMDLERERGITIKSHAIQMDYVHEGEEFVLNLIDTPGHVDFSYEVSRSIAACEGALLVVDAAQSIQAQTISNLYLALENDLEIIPVLNKVDLPSANPEEVTDDIVDLLGCDPSEVIPASAKTGLGIKEILDAIINRVPAPSGKVDAPLRALIFDSVYNPFRGVETYFRVINGSIKKGEKIKFVATGKTYDADEVGTLRLKQFPRKEIKTGDVGYLITGIKDAREVKVGDTITSAVNPTTEAVAGFEDVKPMVFAGIYPVDTEDYEDLRSSMEKLQLNDASLVFLPESSAALGFGFRCGFLGMLHLEIIQERLEREFDMTVITTVPNVSYHAYTNKNPDDVILVNNPSDLPEPSSLNRVEEPFIKATIITKADYVGNVMGLCIEKRGEITNQTYLTTERVELTFDMPLAEIVFDFYDRLKTVSRGYASFDYTPIGLRESKLVKVDVLLNANIVDALSALLHVDNAYDIGKKMCEKLKELIPRQQFDIPIQAAIGAKIIARETVKALRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLK 595          

HSP 2 Score: 163.31 bits (412), Expect = 1.363e-41
Identity = 75/89 (84.27%), Postives = 86/89 (96.63%), Query Frame = 0

Query:  583 LASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            +  K+CEKLKEL+PRQQF+I IQAAIG+KIIARE+V+A+RKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQ+GNVE+PQEAFMAVLK+N
Sbjct:  509 IGKKMCEKLKELIPRQQFDIPIQAAIGAKIIARETVKALRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKLN 597          

BLAST of gtp-binding protein vs. SwissProt
Match: gi|238691486|sp|B2RKK1.1|LEPA_PORG3 (RecName: Full=Elongation factor 4; Short=EF-4; AltName: Full=Ribosomal back-translocase LepA)

HSP 1 Score: 835.869 bits (2158), Expect = 0.000e+0
Identity = 390/572 (68.18%), Postives = 483/572 (84.44%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLE+T TV+ +++Q+Q+LD+MDLERERGITIKSHAIQMD+  DG  Y LNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGI+AQTISNL++A+E+DL IIP++NK+DLP AM EEV+DQ+++L+G DR +I+ AS K G G+D IL AI+++VPAP GDPDAPLQ LIFDSVFN +RGI   F+V NGSI KGD VKF+AT KEY+ADE+G LRL  +P+  ++ GDVGY+ISGIK ++EVKVGDTITH+ + A   I GFEEVKPMVFAG+YP+E  DFE LR  +EKLQLNDA+L ++PE+S ALGFGFRCGFLG+LHMEIVQERL+REF+M VITTVP+V + + + KG    V  PS +P+P LI  +EEPFI A +IT   Y+GPI+TLC+ KRGV+  Q Y++ DRVE+ +D+PL E+V DF+DKLK+IS+GYAS DY L  +RESKLVKLDILLNG+PVDALS + HVD +  +G+++CEKLKEL+PRQQFEIAIQAAIG+KIIARE+++ +RKDVTAKCYGGDISRKRKLLEKQK+GKKRM+QIG VEVPQ+AF+A+ + D
Sbjct:   24 LLEYTNTVSGKDLQDQVLDNMDLERERGITIKSHAIQMDYEMDGEKYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIQAQTISNLYMAIENDLTIIPIVNKVDLPSAMPEEVEDQIIELLGCDRSEIIRASGKTGQGVDQILRAIVEQVPAPAGDPDAPLQCLIFDSVFNPFRGIIAYFKVVNGSIRKGDHVKFIATEKEYDADEVGVLRLDMEPRSEVKTGDVGYIISGIKTSREVKVGDTITHVAKPAKEAIAGFEEVKPMVFAGVYPIEAEDFENLRTSLEKLQLNDASLTFQPESSVALGFGFRCGFLGLLHMEIVQERLDREFNMNVITTVPNVSYKVYDKKGGCKEVHNPSGLPEPTLIDHIEEPFIRASVITNTAYIGPIMTLCLGKRGVLVKQEYISGDRVEIFYDLPLGEIVIDFYDKLKSISKGYASFDYHLHDFRESKLVKLDILLNGEPVDALSTLTHVDNSVTFGQRMCEKLKELIPRQQFEIAIQAAIGAKIIARETIKPVRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKQIGTVEVPQKAFLAVLKLD 595          

HSP 2 Score: 152.14 bits (383), Expect = 8.700e-38
Identity = 70/89 (78.65%), Postives = 84/89 (94.38%), Query Frame = 0

Query:  583 LASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
               ++CEKLKEL+PRQQFEIAIQAAIG+KIIARE+++ +RKDVTAKCYGGDISRKRKLLEKQK+GKKRM+QIG VEVPQ+AF+AVLK++
Sbjct:  507 FGQRMCEKLKELIPRQQFEIAIQAAIGAKIIARETIKPVRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKQIGTVEVPQKAFLAVLKLD 595          

BLAST of gtp-binding protein vs. SwissProt
Match: gi|46396301|sp|Q7MV56.1|LEPA_PORGI (RecName: Full=Elongation factor 4; Short=EF-4; AltName: Full=Ribosomal back-translocase LepA)

HSP 1 Score: 832.402 bits (2149), Expect = 0.000e+0
Identity = 389/572 (68.01%), Postives = 482/572 (84.27%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLE+T TV+ +++Q+Q+LD+MDLERERGITIKSHAIQMD+  DG  Y LNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGI+AQTISNL++A+E+DL IIP++NK+DLP AM EEV+DQ+++L+G DR +I+ AS K G G+D IL AI+++VPAP GDPDAPLQ LIFDSVFN +RGI   F+V NGSI KGD VKF+AT KEY+ADE+G LRL  +P+  ++ GDVGY+ISGIK ++EVKVGDTITH+ + A   I GFEEVKPMVFAG+YP+E  DFE LR  +EKLQLNDA+L ++PE+S ALGFGFRCGFLG+LHMEIVQERL+REF+M VITTVP+V + + + KG    V  PS + +P LI  +EEPFI A +IT   Y+GPI+TLC+ KRGV+  Q Y++ DRVE+ +D+PL E+V DF+DKLK+IS+GYAS DY L  +RESKLVKLDILLNG+PVDALS + HVD +  +G+++CEKLKEL+PRQQFEIAIQAAIG+KIIARE+++ +RKDVTAKCYGGDISRKRKLLEKQK+GKKRM+QIG VEVPQ+AF+A+ + D
Sbjct:   24 LLEYTNTVSGKDLQDQVLDNMDLERERGITIKSHAIQMDYEMDGEKYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIQAQTISNLYMAIENDLTIIPIVNKVDLPSAMPEEVEDQIIELLGCDRSEIIRASGKTGQGVDQILRAIVEQVPAPAGDPDAPLQCLIFDSVFNPFRGIIAYFKVVNGSIRKGDHVKFIATEKEYDADEVGVLRLDMEPRSEVKTGDVGYIISGIKTSREVKVGDTITHVAKPAKEAIAGFEEVKPMVFAGVYPIEAEDFENLRASLEKLQLNDASLTFQPESSVALGFGFRCGFLGLLHMEIVQERLDREFNMNVITTVPNVSYKVYDKKGGCKEVHNPSGLLEPTLIDHIEEPFIRASVITNTAYIGPIMTLCLGKRGVLVKQEYISGDRVEIFYDLPLGEIVIDFYDKLKSISKGYASFDYHLHDFRESKLVKLDILLNGEPVDALSTLTHVDNSVTFGRRMCEKLKELIPRQQFEIAIQAAIGAKIIARETIKPVRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKQIGTVEVPQKAFLAVLKLD 595          

HSP 2 Score: 151.754 bits (382), Expect = 1.091e-37
Identity = 70/89 (78.65%), Postives = 84/89 (94.38%), Query Frame = 0

Query:  583 LASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
               ++CEKLKEL+PRQQFEIAIQAAIG+KIIARE+++ +RKDVTAKCYGGDISRKRKLLEKQK+GKKRM+QIG VEVPQ+AF+AVLK++
Sbjct:  507 FGRRMCEKLKELIPRQQFEIAIQAAIGAKIIARETIKPVRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKQIGTVEVPQKAFLAVLKLD 595          

BLAST of gtp-binding protein vs. SwissProt
Match: gi|166224505|sp|A6LC18.1|LEPA_PARD8 (RecName: Full=Elongation factor 4; Short=EF-4; AltName: Full=Ribosomal back-translocase LepA)

HSP 1 Score: 831.632 bits (2147), Expect = 0.000e+0
Identity = 392/572 (68.53%), Postives = 481/572 (84.09%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLE+T TV  ++MQ Q+LDDMDLERERGITIKSHAIQM +   G  Y LNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGI+AQTISNL++A+E+DLEIIPV+NKIDLP AM EEV+DQ+V+L+G  REDI+ AS K G G+ +ILN I+++VPAPKGDP+APLQ LIFDSVFN +RGI   F+V NG I KGD VKF+ATGKEY+ADE+G L+L   P++ I  GDVGY+ISGIK ++EV+VGDTITH+ R A   I GFEEVKPMVFAG+YP+++ DFE LR  +EKLQLNDA+L ++PE+SAALGFGFRCGFLG+LHMEIVQERL+REF+M VITTVP+V + +   KGE+  V  P  +PDP LI  ++EPFI A +IT   Y+GPI+TLC+ KRGV+  Q Y++ DRVE+ +D+PL E+V DF+DKLK+IS+GYAS DY L  +R SKL KLDILLNG+PVDALS + HVD +  +G+++CEKLKEL+PRQQF+IAIQAAIG+KIIARE+++A+RKDVTAKCYGGDISRKRKLLEKQK+GKKRM+QIG VEVPQ+AF+A+ + D
Sbjct:   24 LLEYTKTVEGKDMQAQVLDDMDLERERGITIKSHAIQMKYNYKGEEYILNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIQAQTISNLYMAIENDLEIIPVMNKIDLPSAMPEEVEDQIVELLGCPREDILRASGKTGEGVTEILNTIVEKVPAPKGDPEAPLQCLIFDSVFNPFRGIIAYFKVVNGVIRKGDHVKFIATGKEYDADEVGILKLDMCPREEIRTGDVGYIISGIKTSREVRVGDTITHVSRPAKDAIAGFEEVKPMVFAGVYPIDSEDFENLRASLEKLQLNDASLTFQPESSAALGFGFRCGFLGLLHMEIVQERLDREFNMDVITTVPNVSYIVHTKKGEEIEVHNPGGLPDPTLIDHIDEPFIRASVITNTTYIGPIMTLCLGKRGVLLKQEYISGDRVEIHYDLPLGEIVIDFYDKLKSISKGYASFDYHLHDFRPSKLAKLDILLNGEPVDALSTLTHVDNSVTFGRRMCEKLKELIPRQQFDIAIQAAIGAKIIARETIKAVRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKQIGTVEVPQKAFLAVLKLD 595          

HSP 2 Score: 152.14 bits (383), Expect = 8.144e-38
Identity = 70/89 (78.65%), Postives = 85/89 (95.51%), Query Frame = 0

Query:  583 LASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
               ++CEKLKEL+PRQQF+IAIQAAIG+KIIARE+++A+RKDVTAKCYGGDISRKRKLLEKQK+GKKRM+QIG VEVPQ+AF+AVLK++
Sbjct:  507 FGRRMCEKLKELIPRQQFDIAIQAAIGAKIIARETIKAVRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKQIGTVEVPQKAFLAVLKLD 595          

BLAST of gtp-binding protein vs. SwissProt
Match: gi|189046440|sp|A5FLU8.1|LEPA_FLAJ1 (RecName: Full=Elongation factor 4; Short=EF-4; AltName: Full=Ribosomal back-translocase LepA)

HSP 1 Score: 831.632 bits (2147), Expect = 0.000e+0
Identity = 400/574 (69.69%), Postives = 482/574 (83.97%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKF-NITNNKGEK-FSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LL  T TVT RE + QLLD+MDLERERGITIKSHAIQM++   G  Y LNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQ I+AQTISNL+LALE+DLEIIPV+NK+DLP A  EEV D ++DL+G   EDI+ AS K G G+++IL AII+++P PKGDP+ PLQ LIFDSV+N +RGIEV+FRV NG I KG K+KFMAT  EY ADEIG+L+L + PK+ +  GDVGYLISGIK A+EVKVGDTIT  K     MI GFE+VKPMVFAGIYPV+T D+E+LR  MEKLQLNDA+L++ PE+SAALGFGFRCGFLGMLHMEI+QERLEREFDMTVITTVP+V +   T  + EK   V+ PS++P+P+ +  +EEPFI A IIT AD+VG +++LC+ KRG+I NQ YLT++RVEL FDMPLAE+VFDF+D+LKT+S+GYAS DY+  G R SKLVKLD+LLN   VDALSA++H D AY+ GK++ EKL+EL+PRQQF+I IQAAIG+KIIARE+++A+RKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQ+GNVE+PQEAFMA+ + +
Sbjct:   24 LLGATQTVTAREEKAQLLDNMDLERERGITIKSHAIQMEYKYKGEEYILNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQSIQAQTISNLYLALENDLEIIPVLNKVDLPSANPEEVSDDIIDLLGCKLEDIIHASGKTGFGVENILAAIIEKIPPPKGDPEEPLQALIFDSVYNPFRGIEVIFRVVNGEIKKGQKIKFMATDNEYFADEIGTLKLNQVPKNVVSAGDVGYLISGIKEAREVKVGDTITDAKVPTTNMITGFEDVKPMVFAGIYPVDTEDYEDLRSSMEKLQLNDASLVFTPESSAALGFGFRCGFLGMLHMEIIQERLEREFDMTVITTVPNVSYLAYTKKEPEKALIVNNPSDLPEPSKLDRVEEPFIKATIITKADFVGNVMSLCIEKRGLITNQTYLTTERVELNFDMPLAEIVFDFYDRLKTVSKGYASFDYSPIGMRTSKLVKLDVLLNAQTVDALSALIHEDNAYNIGKKMTEKLRELIPRQQFDIPIQAAIGAKIIARETIKALRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKLN 597          

HSP 2 Score: 157.532 bits (397), Expect = 1.132e-39
Identity = 72/91 (79.12%), Postives = 86/91 (94.51%), Query Frame = 0

Query:  581 HFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            + +  K+ EKL+EL+PRQQF+I IQAAIG+KIIARE+++A+RKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQ+GNVE+PQEAFMAVLK+N
Sbjct:  507 YNIGKKMTEKLRELIPRQQFDIPIQAAIGAKIIARETIKALRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKLN 597          

BLAST of gtp-binding protein vs. SwissProt
Match: gi|166223194|sp|A6H1S4.1|LEPA_FLAPJ (RecName: Full=Elongation factor 4; Short=EF-4; AltName: Full=Ribosomal back-translocase LepA)

HSP 1 Score: 824.698 bits (2129), Expect = 0.000e+0
Identity = 397/574 (69.16%), Postives = 478/574 (83.28%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFS--VSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LL  T TVT RE + QLLD+MDLERERGITIKSHAIQM++T  G  Y LNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQ I+AQTISNL+LALE+DLEIIPV+NK+DLP A  EEV D ++DL+G   EDI+ AS K G G+++IL AII+++P PKG+ + PLQ LIFDS +N +RGIEV+FRVKNG I KG K+KFMATG EY ADEIG+L+L + PK+ I  GDVGYLISGIK AKEVKVGDT+T  K     MI GFE+VKPMVFAGIYPV+T D+EELR+ MEKLQLNDA+L++  E+SAALGFGFRCGFLGMLHMEI+QERLEREF+MTVITTVP+V +     K  +    V+ P+++P+P+ +  +EEP+I A IIT AD+VG ++ LC+ KRGVI NQ YLT++RVEL FDMPLAE+VFDF+D+LKT+S+GYAS DY+  G R SKLVKLD+LLN   VDALSA++H   AY  GK++CEKLKEL+PRQQF+I IQAAIG+KIIARE+++A+RKDVTAKCYGGDISRKRKLLEKQKKGKKRMR +GNVE+PQEAFMA+ + +
Sbjct:   24 LLGATQTVTAREEKAQLLDNMDLERERGITIKSHAIQMEYTYQGQEYILNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQSIQAQTISNLYLALENDLEIIPVLNKVDLPSANPEEVSDDIIDLLGCKLEDIIHASGKTGFGVENILAAIIEKIPHPKGNIEEPLQALIFDSHYNPFRGIEVIFRVKNGQIKKGQKIKFMATGNEYFADEIGTLKLNQVPKNVISAGDVGYLISGIKEAKEVKVGDTLTDAKTPTTNMIVGFEDVKPMVFAGIYPVDTDDYEELRNSMEKLQLNDASLVFAAESSAALGFGFRCGFLGMLHMEIIQERLEREFNMTVITTVPNVSYLAYTKKEPEVGMIVNNPTDLPEPSKLDRVEEPYIKATIITKADFVGNVMGLCIEKRGVITNQTYLTTERVELNFDMPLAEIVFDFYDRLKTVSKGYASFDYSPIGMRTSKLVKLDVLLNATSVDALSALIHESNAYHIGKKMCEKLKELIPRQQFDIPIQAAIGAKIIARETIKALRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRLVGNVEIPQEAFMAVLKLN 597          

HSP 2 Score: 160.229 bits (404), Expect = 1.254e-40
Identity = 73/91 (80.22%), Postives = 86/91 (94.51%), Query Frame = 0

Query:  581 HFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            + +  K+CEKLKEL+PRQQF+I IQAAIG+KIIARE+++A+RKDVTAKCYGGDISRKRKLLEKQKKGKKRMR +GNVE+PQEAFMAVLK+N
Sbjct:  507 YHIGKKMCEKLKELIPRQQFDIPIQAAIGAKIIARETIKALRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRLVGNVEIPQEAFMAVLKLN 597          

BLAST of gtp-binding protein vs. SwissProt
Match: gi|166223170|sp|A6L744.1|LEPA_BACV8 (RecName: Full=Elongation factor 4; Short=EF-4; AltName: Full=Ribosomal back-translocase LepA)

HSP 1 Score: 822.772 bits (2124), Expect = 0.000e+0
Identity = 384/572 (67.13%), Postives = 477/572 (83.39%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFT T+   E   Q+LDDMDLE+ERGITIKSHAIQM++   G  Y LNLIDTPGHVDFSYEVSRSIAACEGALLIVDA+QG++AQTISNL++ALEHDLEIIPV+NK D+  AM +EV+D+++DL+G   EDI+ AS K G+G+++IL A+++R+P P GD +APLQ LIFDSVFNS+RGI   F+++NG I KGDKVKF  TGKEY+ADEIG L++   P+  +  GDVGY+ISGIK ++EVKVGDTITH+ R  +  I GFEEVKPMVFAG+YP+E  D+E LR  +EKLQLNDA+L ++PE+S ALGFGFRCGFLG+LHMEIVQERL+REFDM VITTVP+V + + + +G    V  P  MPDP LI  +EEPFI+A IITA DY+GPI+TLC+ KRG +  Q Y++ +RVE+ + MPL E+V DF+DKLK+IS+GYAS DY  SG+R SKLVKLDILLNG+PVDALS + HVD AY+ GK++CEKLKEL+PRQQF+IAIQAAIG+KII+RE+++A+RKDVTAKCYGGD+SRKRKLLEKQK+GKKRM+QIGNVEVPQ+AF+A+ + D
Sbjct:   24 LLEFTKTIQVTE--GQMLDDMDLEKERGITIKSHAIQMEYEYGGEKYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVDASQGVQAQTISNLYMALEHDLEIIPVLNKCDMASAMPDEVEDEIIDLLGCKHEDIIRASGKTGMGVEEILKAVVERIPHPTGDEEAPLQALIFDSVFNSFRGIIAYFKIENGVIRKGDKVKFFNTGKEYDADEIGVLKMDMIPRAELRTGDVGYIISGIKTSREVKVGDTITHIARPCEKAIAGFEEVKPMVFAGVYPIEAEDYENLRASLEKLQLNDASLTFQPESSLALGFGFRCGFLGLLHMEIVQERLDREFDMNVITTVPNVSYMVYDKQGHANEVHNPGGMPDPTLIDHIEEPFIDATIITATDYIGPIMTLCLGKRGELVRQNYVSGNRVEIHYKMPLGEIVIDFYDKLKSISKGYASFDYHQSGFRPSKLVKLDILLNGEPVDALSTLTHVDNAYNLGKRMCEKLKELIPRQQFDIAIQAAIGAKIISRETIKAVRKDVTAKCYGGDVSRKRKLLEKQKRGKKRMKQIGNVEVPQKAFLAVLKLD 593          

HSP 2 Score: 155.606 bits (392), Expect = 4.752e-39
Identity = 74/111 (66.67%), Postives = 93/111 (83.78%), Query Frame = 0

Query:  561 PQEAFMALARTDLRKLVKYTHFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            P +A   L   D        + L  ++CEKLKEL+PRQQF+IAIQAAIG+KII+RE+++A+RKDVTAKCYGGD+SRKRKLLEKQK+GKKRM+QIGNVEVPQ+AF+AVLK++
Sbjct:  489 PVDALSTLTHVD------NAYNLGKRMCEKLKELIPRQQFDIAIQAAIGAKIISRETIKAVRKDVTAKCYGGDVSRKRKLLEKQKRGKKRMKQIGNVEVPQKAFLAVLKLD 593          

BLAST of gtp-binding protein vs. SwissProt
Match: gi|33112364|sp|Q8AA33.1|LEPA_BACTN (RecName: Full=Elongation factor 4; Short=EF-4; AltName: Full=Ribosomal back-translocase LepA)

HSP 1 Score: 805.438 bits (2079), Expect = 0.000e+0
Identity = 384/572 (67.13%), Postives = 482/572 (84.27%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFT T+  +    Q+LDDMDLE+ERGITIKSHAIQM++   G  Y LNLIDTPGHVDFSYEVSRSIAACEGALLIVDA+QG++AQTISNL++A+EHDLEIIP+INK D+  AM EEV+D++V+L+G  R++I+ AS K G+G+++IL A+I+R+P P+GD +APLQ LIFDSVFNS+RGI   F+++NG I KGDKVKF  TGKEY+ADE+G L+++  P++ +  GDVGY+ISGIK +KEVKVGDTITH+ R  D  I GFEEVKPMVFAG+YP+E  DFE+LR  +EKLQLNDA+L ++PE+S ALGFGFRCGFLG+LHMEIVQERL+REFDM VITTVP+V +NI + +G    V  P  MPDP LI  +EEP+I A IIT  DY+GPI+TLC+ KRG +  Q Y++ +RVE+ ++MPL E+V DF+D+LK+IS+GYAS DY  +G+R SKLVKLDI+LNG+PVDALS ++H D AYD G+++CEKLKEL+PRQQFEIAIQAAIG+KIIARE+++A+RKDVTAKCYGGD+SRKRKLLEKQKKGKKRM+QIGNVEVPQ+AF+A+ + D
Sbjct:   24 LLEFTNTI--QVTNGQMLDDMDLEKERGITIKSHAIQMEYNYKGEKYILNLIDTPGHVDFSYEVSRSIAACEGALLIVDASQGVQAQTISNLYMAIEHDLEIIPIINKCDMASAMPEEVEDEIVELLGCKRDEIIRASGKTGMGVEEILAAVIERIPHPEGDEEAPLQALIFDSVFNSFRGIIAYFKIENGVIRKGDKVKFFNTGKEYDADEVGVLKMELVPRNELRTGDVGYIISGIKTSKEVKVGDTITHVARPCDKAIAGFEEVKPMVFAGVYPIEAEDFEDLRASLEKLQLNDASLTFQPESSLALGFGFRCGFLGLLHMEIVQERLDREFDMNVITTVPNVSYNIYDKQGNMKEVHNPGGMPDPTLIDHIEEPYIKASIITTTDYIGPIMTLCLGKRGELLKQEYISGNRVEIYYNMPLGEIVIDFYDRLKSISKGYASFDYHPNGFRPSKLVKLDIMLNGEPVDALSTLIHFDNAYDMGRRMCEKLKELIPRQQFEIAIQAAIGAKIIARETIKAVRKDVTAKCYGGDVSRKRKLLEKQKKGKKRMKQIGNVEVPQKAFLAVLKLD 593          

HSP 2 Score: 137.887 bits (346), Expect = 5.719e-33
Identity = 105/269 (39.03%), Postives = 155/269 (57.62%), Query Frame = 0

Query:  411 LTSDRVELTFDM----PLAEVVFDFFDK---LKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIA-RESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTDLRKLVKYTHFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            +  +R++  FDM     +  V ++ +DK   +K +       D TL  + E   +K  I+   D +  +  +         GK+     +E +   + EI     +G  +I   + ++++ K   +  Y  +  R  KL+        ++  + N E P +A   L   D        + +  ++CEKLKEL+PRQQFEIAIQAAIG+KIIARE+++A+RKDVTAKCYGGD+SRKRKLLEKQKKGKKRM+QIGNVEVPQ+AF+AVLK++
Sbjct:  347 IVQERLDREFDMNVITTVPNVSYNIYDKQGNMKEVHNPGGMPDPTLIDHIEEPYIKASIITTTDYIGPIMTLC-------LGKRGELLKQEYISGNRVEIYYNMPLGEIVIDFYDRLKSISKGYASFDYHPNGFRPSKLV--------KLDIMLNGE-PVDALSTLIHFD------NAYDMGRRMCEKLKELIPRQQFEIAIQAAIGAKIIARETIKAVRKDVTAKCYGGDVSRKRKLLEKQKKGKKRMKQIGNVEVPQKAFLAVLKLD 593          

BLAST of gtp-binding protein vs. nr
Match: gi|941703194|ref|WP_055143554.1| (elongation factor 4 [Jiulongibacter sediminis] >gi|936517490|gb|KPM49369.1| elongation factor 4 [Jiulongibacter sediminis])

HSP 1 Score: 1057.74 bits (2734), Expect = 0.000e+0
Identity = 513/572 (89.69%), Postives = 546/572 (95.45%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFTGTVTQREMQ QLLDDMDLERERGITIKSHAIQMD+  +   YTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEH+LEIIPVINKIDLPGAMIEEVKDQVVDLIGID +DIVLASAKEGIGI DIL AI++RVPAPKGDP+APLQGLIFDSVFNSYRGIEV+FRVKNGSI KGDKVKFMATGKEYEADEIGSL+LKKQPKD IECGDVGYLISGIKVAKEVKVGDTITH+ + +  MIKGFEEVKPMVFAGIYPV+TTDFEELR+CMEKLQLNDA+L+WEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKF ITN +G+   VSAPSEMPDPNLI+ LEEPFINAQIITAA+YVGPIITLCM KRGVIKNQ+YLTS+RVELTFD+PLAEVVFDFFDKLK+ISRGYASLDY+LSGYRES LVKLDILLNGDPVDALSAIVH DKAYDWGK+LCEKLKELLPRQQFEIAIQAAIG+K+IARE+VRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIG+VEVPQEAFMA+ + +
Sbjct:   24 LLEFTGTVTQREMQAQLLDDMDLERERGITIKSHAIQMDYLFNDEKYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHNLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDPDDIVLASAKEGIGITDILEAIVNRVPAPKGDPEAPLQGLIFDSVFNSYRGIEVIFRVKNGSIRKGDKVKFMATGKEYEADEIGSLKLKKQPKDVIECGDVGYLISGIKVAKEVKVGDTITHVSKSSPQMIKGFEEVKPMVFAGIYPVDTTDFEELRECMEKLQLNDASLVWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFLITNKRGDVIPVSAPSEMPDPNLIQKLEEPFINAQIITAAEYVGPIITLCMDKRGVIKNQVYLTSNRVELTFDIPLAEVVFDFFDKLKSISRGYASLDYSLSGYRESHLVKLDILLNGDPVDALSAIVHRDKAYDWGKKLCEKLKELLPRQQFEIAIQAAIGAKVIARETVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGSVEVPQEAFMAVLKIN 595          

HSP 2 Score: 169.088 bits (427), Expect = 3.782e-41
Identity = 86/111 (77.48%), Postives = 93/111 (83.78%), Query Frame = 0

Query:  561 PQEAFMALARTDLRKLVKYTHFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            P +A  A+   D        +    KLCEKLKELLPRQQFEIAIQAAIG+K+IARE+VRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIG+VEVPQEAFMAVLKIN
Sbjct:  491 PVDALSAIVHRD------KAYDWGKKLCEKLKELLPRQQFEIAIQAAIGAKVIARETVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGSVEVPQEAFMAVLKIN 595          

BLAST of gtp-binding protein vs. nr
Match: gi|1245964997|ref|WP_096194268.1| (elongation factor 4 [Cytophagales bacterium TFI 002] >gi|1243937029|emb|SOE22762.1| GTP-binding protein LepA [Cytophagales bacterium TFI 002])

HSP 1 Score: 1056.2 bits (2730), Expect = 0.000e+0
Identity = 509/572 (88.99%), Postives = 545/572 (95.28%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFTGTVTQREMQNQLLD+MDLE+ERGITIKSHAIQMD+ +DG  YTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMI+EVKDQVVDLIGI+ + IVLASAKEGIGI DIL+AI+ RVPAPKGDPD PLQ L+FDSVFNSYRGIEVLFRVKNGSI KGDK+KFMATGKEYEADEIGSL+L++QPKDRIECGDVGYLISGIKVAKEVKVGDTITH+ R    MI+GFEEVKPMVFAGIYPV+TT+FEELR+CMEKLQLNDA+L+WEPETSAALGFGFRCGFLGMLHMEIVQERLEREF MTVITTVPSVKF +TN +GEK  VSAPSEMPDPNLI+ LEEPFI+AQIIT+AD+VGPII+LCM KRG+IKNQIYLTS RVELTFD+PLAEVVFDFFD+LKTISRGYASLDY+LSGYRES LVKLDILLNGDPVDALSAIVH DKAYDWGK+LCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMA+ + +
Sbjct:   24 LLEFTGTVTQREMQNQLLDNMDLEKERGITIKSHAIQMDYIKDGEKYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIDEVKDQVVDLIGIEHDSIVLASAKEGIGIQDILDAIVSRVPAPKGDPDGPLQCLVFDSVFNSYRGIEVLFRVKNGSIKKGDKIKFMATGKEYEADEIGSLKLRQQPKDRIECGDVGYLISGIKVAKEVKVGDTITHVARPTTEMIQGFEEVKPMVFAGIYPVDTTEFEELRECMEKLQLNDASLVWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFGMTVITTVPSVKFLVTNKRGEKIPVSAPSEMPDPNLIQTLEEPFISAQIITSADFVGPIISLCMDKRGLIKNQIYLTSTRVELTFDLPLAEVVFDFFDRLKTISRGYASLDYSLSGYRESHLVKLDILLNGDPVDALSAIVHRDKAYDWGKKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 595          

HSP 2 Score: 173.711 bits (439), Expect = 9.227e-43
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0

Query:  586 KLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            KLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN
Sbjct:  510 KLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 595          

BLAST of gtp-binding protein vs. nr
Match: gi|1232128052|gb|OYU67022.1| (elongation factor 4 [Cytophagaceae bacterium BCCC1])

HSP 1 Score: 1019.22 bits (2634), Expect = 0.000e+0
Identity = 495/572 (86.54%), Postives = 530/572 (92.66%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFTGTVT REMQ QLLDDMDLERERGITIKSHAIQMD+   G  Y LNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEH+LEIIPVINKIDLPGAMIEEVKDQ+VDLIGID + IVLASAKEGIGIDDIL AIIDRVPAP GDP A LQGLIFDSVFNSYRGIEV+FRVKNG+I KGDKVKF+ATGKEY ADEIGSL+LKKQPKD +ECGDVGYLISGIKVAKEVKVGDTITH+ R +   I+GFEEVKPMVFAGIYPV+TTDFEELR+ MEKLQLNDA+LIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSV+F +   KGE   VSAPSEMPDPNL+  +EEP+I AQIITA+DYVGPI+ LCM KRG +KNQ+YLTS RVELTFDMPL+EVVFDFFD+LKTISRGYASLDY+L GYRES LVKLD+LLNGDPVDALSAIVH DKAYDWGK+LCEKLKELLPRQQFEIAIQAAIG+KIIARE+V+AMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMA+ + +
Sbjct:   24 LLEFTGTVTAREMQAQLLDDMDLERERGITIKSHAIQMDYVFRGEKYILNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHNLEIIPVINKIDLPGAMIEEVKDQIVDLIGIDPDGIVLASAKEGIGIDDILTAIIDRVPAPVGDPKAELQGLIFDSVFNSYRGIEVIFRVKNGTIRKGDKVKFVATGKEYIADEIGSLKLKKQPKDVVECGDVGYLISGIKVAKEVKVGDTITHVDRPSTQEIQGFEEVKPMVFAGIYPVDTTDFEELRESMEKLQLNDASLIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVRFTVKTRKGEVLQVSAPSEMPDPNLVVSIEEPYIKAQIITASDYVGPIMKLCMEKRGEVKNQVYLTSTRVELTFDMPLSEVVFDFFDRLKTISRGYASLDYSLIGYRESALVKLDVLLNGDPVDALSAIVHRDKAYDWGKKLCEKLKELLPRQQFEIAIQAAIGAKIIARETVKAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 595          

HSP 2 Score: 170.244 bits (430), Expect = 1.330e-41
Identity = 87/111 (78.38%), Postives = 93/111 (83.78%), Query Frame = 0

Query:  561 PQEAFMALARTDLRKLVKYTHFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            P +A  A+   D        +    KLCEKLKELLPRQQFEIAIQAAIG+KIIARE+V+AMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN
Sbjct:  491 PVDALSAIVHRD------KAYDWGKKLCEKLKELLPRQQFEIAIQAAIGAKIIARETVKAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 595          

BLAST of gtp-binding protein vs. nr
Match: gi|503173441|ref|WP_013408102.1| (elongation factor 4 [Leadbetterella byssophila] >gi|311906612|gb|ADQ17053.1| GTP-binding protein LepA [Leadbetterella byssophila DSM 17132])

HSP 1 Score: 1018.07 bits (2631), Expect = 0.000e+0
Identity = 492/572 (86.01%), Postives = 533/572 (93.18%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFTGTV+ R+MQ QLLDDMDLERERGITIKSHAIQM++   G  YTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALE++LEIIPVINKIDLPGAMIEEVKDQ+VDLIGID E IVLASAKEGIGI DIL A++ RVPAP GDP+AP QGLIFDSVFNSYRGIEV+FRVKNGSI KGDKVKF+ATGKEY ADEIGSL+LKKQPKD IECGDVGYLISGIKVAKEVKVGDTITH+ R +   IKGFEEVKPMVFAGIYPVETTDFEELRD M+KLQLNDA+L+WEPETSAALGFGFRCGFLGMLHMEI+QERLEREFDMTVITTVPSVKF +   + E+  VSAPSEMPDPNLI+ +EEPFINAQIITAADYVGPI+ LCM KRGV+KNQ+YLTS RVELTFDMPL+EVVFDFFDKLK+ISRGYASLDYTL GYRES LVKLD+LLNGDPVDALSAIVH DKAY+WGK+LCEKLKELLPRQQFEIAIQAAIG+K+IARE+V+A+RKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIG+VEVPQEAFMA+ + +
Sbjct:   24 LLEFTGTVSARDMQAQLLDDMDLERERGITIKSHAIQMNYEFQGEQYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEYNLEIIPVINKIDLPGAMIEEVKDQIVDLIGIDPEKIVLASAKEGIGITDILEAVVTRVPAPTGDPNAPFQGLIFDSVFNSYRGIEVIFRVKNGSIRKGDKVKFIATGKEYIADEIGSLKLKKQPKDVIECGDVGYLISGIKVAKEVKVGDTITHVDRPSTEPIKGFEEVKPMVFAGIYPVETTDFEELRDSMDKLQLNDASLVWEPETSAALGFGFRCGFLGMLHMEIIQERLEREFDMTVITTVPSVKFLVNTKRKEQIQVSAPSEMPDPNLIESIEEPFINAQIITAADYVGPIMKLCMDKRGVVKNQVYLTSTRVELTFDMPLSEVVFDFFDKLKSISRGYASLDYTLIGYRESNLVKLDVLLNGDPVDALSAIVHRDKAYEWGKKLCEKLKELLPRQQFEIAIQAAIGAKVIARETVKAIRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGSVEVPQEAFMAVLKIN 595          

HSP 2 Score: 166.007 bits (419), Expect = 4.600e-40
Identity = 84/111 (75.68%), Postives = 93/111 (83.78%), Query Frame = 0

Query:  561 PQEAFMALARTDLRKLVKYTHFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            P +A  A+   D        +    KLCEKLKELLPRQQFEIAIQAAIG+K+IARE+V+A+RKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIG+VEVPQEAFMAVLKIN
Sbjct:  491 PVDALSAIVHRD------KAYEWGKKLCEKLKELLPRQQFEIAIQAAIGAKVIARETVKAIRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGSVEVPQEAFMAVLKIN 595          

BLAST of gtp-binding protein vs. nr
Match: gi|1325848383|ref|WP_101727083.1| (elongation factor 4 [Emticicia sp. TH156] >gi|1319730786|gb|PLK43743.1| elongation factor 4 [Emticicia sp. TH156])

HSP 1 Score: 964.14 bits (2491), Expect = 0.000e+0
Identity = 462/570 (81.05%), Postives = 519/570 (91.05%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALAR 570
            LL+ T T++ R+MQ Q+LDDMDLERERGITIKSHAIQM++T +G  + LNLIDTPGHVDFSYEVSRSIAACEGALL+VDA+QGIEAQTISNLFLAL+HDLEIIPV+NKIDLPGAM EEVKDQ+VDLIG DR+ I+ ASAKEGIGI++IL AII R+ AP G+P A LQ LIFDS FNSYRGIEV+FRVKNG I+KGDKVKF+ATGKEY ADE+G L+L+K+ +D ++CGDVGY+ISGIKVAKEVKVGDTITH +      IKGFEEVKPMVFAGIYPVET++FE+LR+ MEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTV+TTVPSV+FN+ + KGE   VSAPSEMPDPN I  +EEPFINAQIITAADYVGPI+ LCM KRG++KNQ+YLT+ RVELTF+MPL+EVVFDFFDKLKTISRGYASLDY L GYRES LVKLDILLNG+PVDALSAIVH DKAYDWGK+LCEKL+ELLPRQQFEIAIQAAIG+KIIARE+V+A+RKDV AKCYGGDISRKRKLLEKQKKGKKRMRQIG VEVPQEAFMA+ +
Sbjct:   24 LLQSTNTISDRDMQAQVLDDMDLERERGITIKSHAIQMNYTFEGEQFILNLIDTPGHVDFSYEVSRSIAACEGALLVVDASQGIEAQTISNLFLALDHDLEIIPVLNKIDLPGAMPEEVKDQIVDLIGCDRDSIIPASAKEGIGINEILEAIIKRIKAPVGNPKAELQALIFDSQFNSYRGIEVIFRVKNGRISKGDKVKFVATGKEYIADEVGILKLQKEARDFVDCGDVGYIISGIKVAKEVKVGDTITHAENPTKEAIKGFEEVKPMVFAGIYPVETSEFEDLREAMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVLTTVPSVRFNVIDKKGETLEVSAPSEMPDPNYIDWIEEPFINAQIITAADYVGPIMGLCMEKRGILKNQVYLTATRVELTFEMPLSEVVFDFFDKLKTISRGYASLDYHLIGYRESNLVKLDILLNGEPVDALSAIVHRDKAYDWGKKLCEKLRELLPRQQFEIAIQAAIGAKIIARETVKALRKDVIAKCYGGDISRKRKLLEKQKKGKKRMRQIGTVEVPQEAFMAVLK 593          

HSP 2 Score: 160.614 bits (405), Expect = 3.011e-38
Identity = 78/86 (90.70%), Postives = 84/86 (97.67%), Query Frame = 0

Query:  586 KLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            KLCEKL+ELLPRQQFEIAIQAAIG+KIIARE+V+A+RKDV AKCYGGDISRKRKLLEKQKKGKKRMRQIG VEVPQEAFMAVLKI+
Sbjct:  510 KLCEKLRELLPRQQFEIAIQAAIGAKIIARETVKALRKDVIAKCYGGDISRKRKLLEKQKKGKKRMRQIGTVEVPQEAFMAVLKIS 595          

BLAST of gtp-binding protein vs. nr
Match: gi|504843688|ref|WP_015030790.1| (elongation factor 4 [Emticicia sp. MM] >gi|387857005|gb|AFK05102.1| GTP-binding protein lepA [Emticicia oligotrophica DSM 17448])

HSP 1 Score: 957.207 bits (2473), Expect = 0.000e+0
Identity = 455/572 (79.55%), Postives = 521/572 (91.08%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LL+FTGTV+ R+MQ Q+LDDMDLERERGITIKSHAIQM++  +G  Y LNLIDTPGHVDFSYEVSRSIAACEGALL+VDAAQGIEAQTISNLFLALEH+LEIIP++NKIDLPGAM EEVKDQ+VDLIG DR+ I+ ASAKEGIGI++IL AII R+  P GDP+A LQ LIFDS FNS+RGIEV+FRVKNG I KGDKVKF+ATGKEY ADE+G LRL K+P+D ++CGDVGY+ISGIKVAKEVKVGDTIT+ +     +++GF+EVKPMVFAGIYPV+T++FE+LRD MEKLQLNDA+LIWEPETSAALGFGFRCGFLGMLHMEI+QERLEREFDMTV+TTVPSVKF++ + K E   VSAPSEMPDPN I  +EEPFI+AQIITAADYVGPII LCM KRG++KNQ+YLTS RVELTFD+PL+EVVFDFFDKLK+ISRGYASLDY L GYRES LVKLDILLNG+PVDALSAIVH DKAY+WGK+LCEKL+ELLPRQQF+IAIQAAIG+K+I+RE+V+A+RKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIG VEVPQ+AFMA+ + +
Sbjct:   24 LLQFTGTVSARDMQAQVLDDMDLERERGITIKSHAIQMNYQFEGQEYVLNLIDTPGHVDFSYEVSRSIAACEGALLVVDAAQGIEAQTISNLFLALEHNLEIIPILNKIDLPGAMPEEVKDQIVDLIGCDRDSIIPASAKEGIGINEILAAIIHRIKPPVGDPNAELQALIFDSTFNSFRGIEVIFRVKNGRINKGDKVKFLATGKEYYADEVGILRLSKEPRDYVDCGDVGYIISGIKVAKEVKVGDTITNAENPTKEIVQGFQEVKPMVFAGIYPVDTSEFEDLRDAMEKLQLNDASLIWEPETSAALGFGFRCGFLGMLHMEIIQERLEREFDMTVLTTVPSVKFHVVDKKNEIIEVSAPSEMPDPNGISWIEEPFISAQIITAADYVGPIIGLCMDKRGLLKNQVYLTSTRVELTFDLPLSEVVFDFFDKLKSISRGYASLDYHLIGYRESHLVKLDILLNGEPVDALSAIVHRDKAYEWGKKLCEKLRELLPRQQFDIAIQAAIGAKVISRETVKALRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGTVEVPQDAFMAILKIN 595          

HSP 2 Score: 160.229 bits (404), Expect = 3.905e-38
Identity = 79/111 (71.17%), Postives = 92/111 (82.88%), Query Frame = 0

Query:  561 PQEAFMALARTDLRKLVKYTHFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            P +A  A+   D        +    KLCEKL+ELLPRQQF+IAIQAAIG+K+I+RE+V+A+RKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIG VEVPQ+AFMA+LKIN
Sbjct:  491 PVDALSAIVHRD------KAYEWGKKLCEKLRELLPRQQFDIAIQAAIGAKVISRETVKALRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGTVEVPQDAFMAILKIN 595          

BLAST of gtp-binding protein vs. nr
Match: gi|947621096|ref|WP_056284121.1| (elongation factor 4 [Dyadobacter sp. Leaf189] >gi|945169806|gb|KQS30800.1| elongation factor 4 [Dyadobacter sp. Leaf189])

HSP 1 Score: 957.207 bits (2473), Expect = 0.000e+0
Identity = 453/572 (79.20%), Postives = 517/572 (90.38%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFT TVTQREMQ QLLD+MDLERERGITIKSHAIQM++   G  Y LNLIDTPGHVDFSYEVSRSIAACEGALL+VDA+QG EAQTISNL+LA+ HDL IIPV+NKIDLPGAM EE+KD++VDL+   REDI+ AS KEGIGI+DILNAI++R+PAP GDP+APLQ LIFDSVFNSYRGIEV+FRVKNGSI KGD+VKFMATGKEY ADEIG+L L++ PK  +ECGDVGYLISGIKVAKEVKVGDT TH+ R A   I GF EVKPMVFAGIYPV+T++FEELR+ MEKLQLNDAAL+WEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSV+F + N K +  ++SAPSEMPDPN IK++EEP+INAQIIT +DY+GPI+ LCM KRG++KNQ+YLTS+RVEL F +PLAEVVFDFFDKLKTISRGYASLDY L+GY+ES +VKLD++LNG+PVDALSAIVH  K+Y+WGK+LCEKL+EL+PRQ FEIAIQAAIG KIIARE+V+AMRKDV AKCYGGDISRKRKLLEKQKKGKKRMRQ+GNVE+PQEAFMA+ + +
Sbjct:   24 LLEFTKTVTQREMQAQLLDNMDLERERGITIKSHAIQMNYIYKGEEYILNLIDTPGHVDFSYEVSRSIAACEGALLLVDASQGTEAQTISNLYLAINHDLVIIPVLNKIDLPGAMPEEIKDEMVDLLDCKREDIIPASGKEGIGIEDILNAIVERIPAPVGDPEAPLQALIFDSVFNSYRGIEVIFRVKNGSIRKGDRVKFMATGKEYIADEIGTLGLQQIPKQVVECGDVGYLISGIKVAKEVKVGDTFTHIDRPAKEAIVGFSEVKPMVFAGIYPVDTSEFEELREAMEKLQLNDAALVWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVQFRVVNTKDKLLNISAPSEMPDPNHIKVIEEPYINAQIITKSDYIGPILNLCMDKRGILKNQVYLTSERVELQFLIPLAEVVFDFFDKLKTISRGYASLDYELAGYQESDMVKLDVMLNGEPVDALSAIVHRSKSYEWGKKLCEKLRELIPRQMFEIAIQAAIGQKIIARETVKAMRKDVLAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKIN 595          

HSP 2 Score: 161.77 bits (408), Expect = 1.442e-38
Identity = 77/92 (83.70%), Postives = 85/92 (92.39%), Query Frame = 0

Query:  580 THFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            ++    KLCEKL+EL+PRQ FEIAIQAAIG KIIARE+V+AMRKDV AKCYGGDISRKRKLLEKQKKGKKRMRQ+GNVE+PQEAFMAVLKIN
Sbjct:  504 SYEWGKKLCEKLRELIPRQMFEIAIQAAIGQKIIARETVKAMRKDVLAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKIN 595          

BLAST of gtp-binding protein vs. nr
Match: gi|1222280976|ref|WP_090334786.1| (elongation factor 4 [Dyadobacter koreensis] >gi|1095199128|emb|SEI59968.1| GTP-binding protein LepA [Dyadobacter koreensis])

HSP 1 Score: 954.51 bits (2466), Expect = 0.000e+0
Identity = 453/572 (79.20%), Postives = 516/572 (90.21%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFT TV+ R+MQ QLLD+MDLERERGITIKSHAIQM +   G  +TLNLIDTPGHVDFSYEVSRSIAACEGALL+VDA+QG EAQTISNL+LA+ H+L IIPV+NK+DLPGA  EE+KD++VDL+G DR+DI+ AS KEGIGI +IL+AII+RVPAP GDP+APLQ LIFDSVFNSYRGIEV+FR+KNGSI KGDKVKFM TGKEY ADEIG+L L + PK  +ECGDVGYLISGIKVAKEVKVGDTITHL RQA   I GF EVKPMVFAGIYPV+TT+FEELRD MEKLQLNDAAL+WEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSV+F ITN K +  ++SAPSEMP+PNLI  +EEP+INAQIIT +DY+GPI+TLCM KRG++KNQ+YLT++RVEL F +PLAEVVFDFFDKLKTIS+GYASLDY L+GY ES +VKLD++LNG+PVDALSAIVH  KAY+WGK+LCEKL+EL+PRQ FEIAIQAAIG KIIARE+V+AMRKDV AKCYGGDISRKRKLLEKQKKGKKRMRQ+GNVE+PQEAFMA+ + +
Sbjct:   24 LLEFTKTVSNRDMQAQLLDNMDLERERGITIKSHAIQMTYLYKGEEFTLNLIDTPGHVDFSYEVSRSIAACEGALLLVDASQGTEAQTISNLYLAINHNLTIIPVLNKVDLPGARPEEIKDEMVDLLGCDRDDIIPASGKEGIGIREILDAIIERVPAPVGDPEAPLQALIFDSVFNSYRGIEVIFRIKNGSIKKGDKVKFMNTGKEYFADEIGTLGLVQIPKQVVECGDVGYLISGIKVAKEVKVGDTITHLNRQASESIVGFSEVKPMVFAGIYPVDTTEFEELRDAMEKLQLNDAALVWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVQFRITNTKDQLINISAPSEMPEPNLINWIEEPYINAQIITKSDYIGPILTLCMDKRGMLKNQVYLTAERVELQFRLPLAEVVFDFFDKLKTISKGYASLDYDLAGYEESDMVKLDVMLNGEPVDALSAIVHRTKAYEWGKKLCEKLRELIPRQMFEIAIQAAIGQKIIARETVKAMRKDVLAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKIN 595          

HSP 2 Score: 161.77 bits (408), Expect = 1.225e-38
Identity = 77/86 (89.53%), Postives = 83/86 (96.51%), Query Frame = 0

Query:  586 KLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            KLCEKL+EL+PRQ FEIAIQAAIG KIIARE+V+AMRKDV AKCYGGDISRKRKLLEKQKKGKKRMRQ+GNVE+PQEAFMAVLKIN
Sbjct:  510 KLCEKLRELIPRQMFEIAIQAAIGQKIIARETVKAMRKDVLAKCYGGDISRKRKLLEKQKKGKKRMRQVGNVEIPQEAFMAVLKIN 595          

BLAST of gtp-binding protein vs. nr
Match: gi|929014335|ref|WP_054038266.1| (elongation factor 4 [bacterium 336/3] >gi|927342485|gb|KOY85913.1| elongation factor 4 [bacterium 336/3])

HSP 1 Score: 951.814 bits (2459), Expect = 0.000e+0
Identity = 453/572 (79.20%), Postives = 518/572 (90.56%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFTGT+ +R+MQ Q+LDDMDLERERGITIKSHAIQM +T +G  YTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDA+QG+EAQTISNL+LA+ +DL IIPV+NKIDLPGAM EE+ DQ+VDLIG  REDI+ AS KEGIGI+DIL A+I RVPAPKG+ D  LQ LIFDSVFNS+RGIEV+FRVKNGSI KGDKVKF+ATGKEY ADE+G L+L+K+P+D+IECGDVGYLISGIK AKEVKVGDTITH+   ++ ++ GFE+VKPMVFAGIYPVET++FE+LR+ MEKLQLNDA+L+WEPETSAALGFGFRCGFLGMLH+EI+QERLEREFDMTVITTVPSV+F + N KGE+  + APSEMPDP  I  +EEPF+  QIIT +DYVG IITLCM KRGVIKNQ+YLTSDRVELTFDMPLAE+VFDFFDKLKTISRGYASLDY L+G+RES +VKLDI+LNG+PVDALSAIVH  KAYDWGK+LCEKL+ELLPRQQFEIAIQAAIG KI+ARE+V+A+RK+V AKCYGGDISRKRKLLEKQKKGKKRMRQIG VEVPQEAFMA+ + +
Sbjct:   24 LLEFTGTIDKRQMQAQVLDDMDLERERGITIKSHAIQMKYTYEGEIYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDASQGVEAQTISNLYLAIGNDLTIIPVLNKIDLPGAMPEEISDQIVDLIGCPREDIIHASGKEGIGIEDILKAVITRVPAPKGEADGKLQALIFDSVFNSFRGIEVIFRVKNGSIKKGDKVKFVATGKEYFADEVGVLKLQKEPRDQIECGDVGYLISGIKQAKEVKVGDTITHMNAASEAIL-GFEDVKPMVFAGIYPVETSEFEDLREAMEKLQLNDASLVWEPETSAALGFGFRCGFLGMLHLEIIQERLEREFDMTVITTVPSVQFKVFNIKGEESRIQAPSEMPDPVHIDYIEEPFVKVQIITKSDYVGAIITLCMDKRGVIKNQVYLTSDRVELTFDMPLAEIVFDFFDKLKTISRGYASLDYELAGWRESDMVKLDIMLNGEPVDALSAIVHRKKAYDWGKRLCEKLRELLPRQQFEIAIQAAIGQKIVARETVKALRKNVIAKCYGGDISRKRKLLEKQKKGKKRMRQIGTVEVPQEAFMAVLKLE 594          

HSP 2 Score: 155.606 bits (392), Expect = 1.490e-36
Identity = 75/93 (80.65%), Postives = 84/93 (90.32%), Query Frame = 0

Query:  578 KYTHFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKI 670
            K  +    +LCEKL+ELLPRQQFEIAIQAAIG KI+ARE+V+A+RK+V AKCYGGDISRKRKLLEKQKKGKKRMRQIG VEVPQEAFMAVLK+
Sbjct:  501 KKAYDWGKRLCEKLRELLPRQQFEIAIQAAIGQKIVARETVKALRKNVIAKCYGGDISRKRKLLEKQKKGKKRMRQIGTVEVPQEAFMAVLKL 593          

BLAST of gtp-binding protein vs. nr
Match: gi|671545710|ref|WP_031529163.1| (elongation factor 4 [Dyadobacter crusticola])

HSP 1 Score: 951.814 bits (2459), Expect = 0.000e+0
Identity = 452/572 (79.02%), Postives = 515/572 (90.03%), Query Frame = 0

Query:    1 LLEFTGTVTQREMQNQLLDDMDLERERGITIKSHAIQMDFTRDGTFYTLNLIDTPGHVDFSYEVSRSIAACEGALLIVDAAQGIEAQTISNLFLALEHDLEIIPVINKIDLPGAMIEEVKDQVVDLIGIDREDIVLASAKEGIGIDDILNAIIDRVPAPKGDPDAPLQGLIFDSVFNSYRGIEVLFRVKNGSIAKGDKVKFMATGKEYEADEIGSLRLKKQPKDRIECGDVGYLISGIKVAKEVKVGDTITHLKRQADTMIKGFEEVKPMVFAGIYPVETTDFEELRDCMEKLQLNDAALIWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVKFNITNNKGEKFSVSAPSEMPDPNLIKLLEEPFINAQIITAADYVGPIITLCMHKRGVIKNQIYLTSDRVELTFDMPLAEVVFDFFDKLKTISRGYASLDYTLSGYRESKLVKLDILLNGDPVDALSAIVHVDKAYDWGKQLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMALARTD 572
            LLEFT TVTQREMQ QLLD+MDLERERGITIKSHAIQM++   G  Y LNLIDTPGHVDFSYEVSRSIAACEGALL+VDA+QG EAQTISNL+LA+ HDL IIPV+NKIDLPGAM EE+KD++VDL+   REDI+ AS KEGIGI+DILNAII+R+PAP GDP+APLQ LIFDSVFNSYRGIEV+FRVKNGSI KGD+VKFMATGKEY ADEIG+L L + PK  +ECGDVGYLISGIKVAKEVKVGDT TH+ R A   I GF EVKPMVFAGIYPV+T++FEELR+ MEKLQLNDAAL+WEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSV+F + N K +  ++SAPSEMPDP  IK++EEP+INAQIIT +DY+GPI+ LCM KRG++KNQ+YLTS+RVEL F +PLAEVVFDFFDKLKTISRGYASLDY L+GY+ES +VKLD++LNG+PVDALSAIVH  K+Y+WGK+LCEKL+EL+PRQ FEIAIQAAIG KIIARE+V+AMRKDV AKCYGGDISRKRKLLEKQKKGKKRMRQ+G+VE+PQEAFMA+ + +
Sbjct:   24 LLEFTKTVTQREMQAQLLDNMDLERERGITIKSHAIQMNYIYKGEEYILNLIDTPGHVDFSYEVSRSIAACEGALLLVDASQGTEAQTISNLYLAINHDLVIIPVLNKIDLPGAMPEEIKDEMVDLLDCKREDIIPASGKEGIGIEDILNAIIERIPAPVGDPEAPLQALIFDSVFNSYRGIEVIFRVKNGSIRKGDRVKFMATGKEYIADEIGTLGLAQIPKQVVECGDVGYLISGIKVAKEVKVGDTFTHIDRPAKEAIVGFSEVKPMVFAGIYPVDTSEFEELREAMEKLQLNDAALVWEPETSAALGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVQFRVINTKDKLLNISAPSEMPDPTQIKVIEEPYINAQIITKSDYIGPILNLCMDKRGILKNQVYLTSERVELQFLIPLAEVVFDFFDKLKTISRGYASLDYELAGYQESDMVKLDVMLNGEPVDALSAIVHRSKSYEWGKKLCEKLRELIPRQMFEIAIQAAIGQKIIARETVKAMRKDVLAKCYGGDISRKRKLLEKQKKGKKRMRQVGSVEIPQEAFMAVLKIN 595          

HSP 2 Score: 159.458 bits (402), Expect = 8.281e-38
Identity = 76/92 (82.61%), Postives = 85/92 (92.39%), Query Frame = 0

Query:  580 THFLASKLCEKLKELLPRQQFEIAIQAAIGSKIIARESVRAMRKDVTAKCYGGDISRKRKLLEKQKKGKKRMRQIGNVEVPQEAFMAVLKIN 671
            ++    KLCEKL+EL+PRQ FEIAIQAAIG KIIARE+V+AMRKDV AKCYGGDISRKRKLLEKQKKGKKRMRQ+G+VE+PQEAFMAVLKIN
Sbjct:  504 SYEWGKKLCEKLRELIPRQMFEIAIQAAIGQKIIARETVKAMRKDVLAKCYGGDISRKRKLLEKQKKGKKRMRQVGSVEIPQEAFMAVLKIN 595