BLAST of conserved unknown protein vs. L. salmonis genes
Match: EMLSAG00000003161 (supercontig:LSalAtl2s:LSalAtl2s1765:94718:97227:1 gene:EMLSAG00000003161 transcript:EMLSAT00000003161 description:"augustus_masked-LSalAtl2s1765-processed-gene-0.1")

HSP 1 Score: 244.588 bits (623), Expect = 6.714e-75
Identity = 132/401 (32.92%), Postives = 238/401 (59.35%), Query Frame = 0

Query:  185 SMKTEELWTRLRDKYVTKAMEEAFDFVTNIIKNLRDFGGIDDEEFDSPLFVLLALIKTAQDEFDQAWINSLPKTSSVDSITFDLHRRVSSHALQVYDASGEEDPKKQAQIMKIPSEKDIVYTYFKDTGD--HVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEF--LDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVLVRYDDDETANNLKRIQDEIMRIKQDRFPFLEHPGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDNC 581
            S   ++LW  +++  ++  ++EA + +  II+N+++   ID   F +PLF+L+AL     D F + ++N +   S + S  F   +++  +A+++Y AS        A+IM I     ++Y  + D+ +  H PKF++F   +  ++ L IRGT S+TDV +D++C D +    +G+AH GI  G+ KV+++   ++ ++LE   R +L+LTGHSLG G +EL+TM +++   +   +D +++CIA+APPPV+R+D ++P ++ +AI+I+IN  D VP + L ++A  ++++R +D L +   + F +L+   ++ T  N++ I++ I    QD FP+LEHPG I Y  K K         + +  +  Q S  F+R++ L + MI++HL   Y  AFDN 
Sbjct:   58 SNNMDQLWVAMKNAVLSPNVQEALNTIDEIIENVKEVDKID---FKTPLFLLVALFYKVAD-FKEEFLNDI---SDLKSNEFFEAKKIGRYAVRIYSASLLVSKAVIAKIMDI-ERSSLIYVSWMDSAEDTHHPKFLIFETDD--TVFLVIRGTFSVTDVFLDLVCSDRKVDKFEGYAHLGIYEGAQKVMEKSMPIISKSLESGNRKKLVLTGHSLGGGASELITMELIQQFEKEGKDDIDIKCIALAPPPVFRSDADIPHKIKNAIKIFINTSDIVPSLSLATVALFISILRQLDNLNLTMENIFYILMGSKNEVTEKNVQNIKEVIQSANQDSFPYLEHPGNIYYFSKTKDG-------TNRTFIKNQVSEYFSRKIRLFDGMIVNHLHGNYEVAFDNV 441          

BLAST of conserved unknown protein vs. L. salmonis genes
Match: EMLSAG00000009318 (supercontig:LSalAtl2s:LSalAtl2s595:47796:52338:1 gene:EMLSAG00000009318 transcript:EMLSAT00000009318 description:"maker-LSalAtl2s595-augustus-gene-0.25")

HSP 1 Score: 118.627 bits (296), Expect = 9.814e-29
Identity = 85/300 (28.33%), Postives = 144/300 (48.00%), Query Frame = 0

Query:  214 IIKNLRDFGGIDDEEFDS---PLFVLLALIKTAQDEFDQAWINSLPKTSSVDSI------TFDLHRRVSSHALQVYDASGEEDPKKQAQI-MKIPSEKDIVYTYFKDT---GDHVPKFILFLDHEIGSLVLSIRGTH-----SLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKA-EYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVLVRYDD 494
            I  +L   G +D+ E D    P  + L LI   Q    +AW N        D +      TF     +     +       ED  +Q +  M    ++DI+Y +  D    GDH P F L L  +   +VL+I GT       + DV +D+  D E FL GHAHRG+  G   +++++   +  TLEE+P Y +++TG+SLGAG ++L+TM   + ++ + IP  T ++C     PPV+ +D     +  +   +  NN D +    L ++ KL   ++A++++ M + D  +++    D
Sbjct:   55 IATDLDGDGLMDEMEEDDRWVPYLLTLGLIYKVQKNDSKAWYNHETSFLQNDDLQEKFLTTFGYFWHLLVEISKCIRTQSSEDVLEQLRTTMTCIPQEDIIYAHSTDQQHLGDHCPDFALTLQRDQKLVVLTICGTRMIPAPEMKDVFMDLYADTEPFLHGHAHRGMAIGXRNIVEKVLPFILSTLEENPGYEIMVTGYSLGAGISQLVTMEFSEGESRKVIPSGTIIRCFCYGTPPVFYSDEVQDYKCPNVYSVTFNN-DGLISASLTNVLKLFMQIKALERMHMRRRDMCRLVFSKSD 353          

BLAST of conserved unknown protein vs. L. salmonis genes
Match: EMLSAG00000004004 (supercontig:LSalAtl2s:LSalAtl2s214:147448:149600:-1 gene:EMLSAG00000004004 transcript:EMLSAT00000004004 description:"augustus_masked-LSalAtl2s214-processed-gene-1.4")

HSP 1 Score: 70.8626 bits (172), Expect = 4.672e-13
Identity = 52/178 (29.21%), Postives = 90/178 (50.56%), Query Frame = 0

Query:  318 FILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGH----AHRGIMRGSCKVLKQIQT--VLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVL 489
            F++ +D E   +V+SIRG+ ++ D I D++ D    +DG+     H G+M  +  + K++Q   +L + L  +P Y +++TGHSLGAG A +LT ++   +  Y        C A A P        +  E    I + +N  D  PR+   + A L+  +R V  L   +  ++K+L
Sbjct:  371 FMVIIDKEKEKIVISIRGSMTMNDAITDLLIDTTA-IDGYEQYYTHTGMMEAARNIYKELQDKDILNKALALNPGYGIVVTGHSLGAGXAVILTFLL---RILY----KSTICYAFACPVCLNERARLESERF-VINV-VNGDDVFPRLSPTNFALLVTQMRHV--LRYSRVPKYKIL 536          

BLAST of conserved unknown protein vs. SwissProt
Match: gi|114149273|sp|P0C1S9.1|DGLB_RAT (RecName: Full=Sn1-specific diacylglycerol lipase beta; Short=DGL-beta)

HSP 1 Score: 61.6178 bits (148), Expect = 1.150e-8
Identity = 43/127 (33.86%), Postives = 67/127 (52.76%), Query Frame = 0

Query:  280 YDA-SGEEDPKKQAQIMKIPS--EKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDE------EFLDGHAHRGIMRGSCKVLKQIQT--VLRQTLEEHPRYRLILTGHS 395
            YDA  G+E     A I+K      +D ++  F D    +P FI+ LDH   S+V+++RGT SL DV+ D+  + E      E  D  AH+GI + +  + +++    +L Q     P YRL++ GHS
Sbjct:  318 YDAVEGDEHHCHFASILKTTGLQYRDFIHVSFHDKVYELP-FIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESENLELDIELQDCVAHKGIAQAARYIYRRLVNDGILSQAFSVAPEYRLVVVGHS 443          

BLAST of conserved unknown protein vs. SwissProt
Match: gi|114149272|sp|Q8NCG7.2|DGLB_HUMAN (RecName: Full=Sn1-specific diacylglycerol lipase beta; Short=DGL-beta; AltName: Full=KCCR13L)

HSP 1 Score: 60.077 bits (144), Expect = 2.882e-8
Identity = 48/138 (34.78%), Postives = 76/138 (55.07%), Query Frame = 0

Query:  301 KDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDE------EFLDGHAHRGIMRGSCKVLKQI--QTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPP 430
            +D ++  F D    +P F++ LDH   S+V+++RGT SL DV+ D+  + E      E  D  AH+GI + +  V +++    +L Q     P YRL++ GHSLG G A LL  ++   +A Y     +V+C A +PP
Sbjct:  342 RDFIHVSFHDKVYELP-FLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDVECEVQDRLAHKGISQAARYVYQRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATML---RAAY----PQVRCYAFSPP 471          

BLAST of conserved unknown protein vs. SwissProt
Match: gi|81902338|sp|Q91WC9.2|DGLB_MOUSE (RecName: Full=Sn1-specific diacylglycerol lipase beta; Short=DGL-beta)

HSP 1 Score: 59.3066 bits (142), Expect = 5.939e-8
Identity = 85/346 (24.57%), Postives = 159/346 (45.95%), Query Frame = 0

Query:  280 YDA-SGEEDPKKQAQIMKIPS--EKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDE------EFLDGHAHRGIMRGSCKVLKQIQT--VLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPP--VYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKL-LAMVRAVDKLEMEKTDQFKVLVR---YD-------------DDETANNLKR--IQDEIMRIKQD--------------RFPFLEHPGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFD 579
            YDA  G++     A I+K      +D ++  F D    +P FI+ LDH   S+V+++RGT SL DV+ D+  + E      E  D  AH+GI + +  + +++    +L Q     P Y+L+L GHSLGAG A LL +++   +  Y     +V+  A +PP   + ++ +E  K+   ++   I   D +PR+ + ++  L   ++R +      K   +K+L+    Y              D+ T   L +  + ++ +  +                ++P L  PG+I +L +   SG+     + +        A F++ + +  +M++DH+    I+A D
Sbjct:  318 YDAVEGDQHNCHFASILKTTGLQYRDFIHISFHDKVYELP-FIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESETLELGIELQDCVAHKGIAQAARYIHRRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIML---RGAY----PQVRAYAFSPPRGLLSKSLYEYSKDFVVSL---ILGMDVIPRLSVTNMEDLKRRILRVIANCNKPK---YKILLHGCWYGLFGGSPDNFPTELDEGTQGALTQPLLGEQTLLTRYSPGYCSSDSPLDSPTKYPTLYPPGRIIHLEEEGGSGRFGCCSAAQYRARWAHEAEFSK-ILIGPKMLIDHMPDVMIRALD 648          

BLAST of conserved unknown protein vs. nr
Match: gi|1325299731|ref|XP_023331719.1| (uncharacterized protein LOC111703874 [Eurytemora affinis])

HSP 1 Score: 244.973 bits (624), Expect = 9.267e-71
Identity = 143/393 (36.39%), Postives = 249/393 (63.36%), Query Frame = 0

Query:  177 REDASEIISMKTEELWTRLRDKYVTKAMEEAFDFVTNIIKNLRDFGGIDDEEFDSPLFVLLALIKTAQDEFDQAWINSLP---KTSSVDSITFDLHRRVSSHALQVYDASGEEDPKKQAQIMKIPSEKDIVYTYFKDTGD--HVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTM-VILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVLVRYDDDETANNLKRIQDEIMRIKQDRFPFLEHPGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLE 563
            +++  E +    E+   ++++   TK M+EA D +++ I+ L+ F  ++ +++ SPLF+L A++K  Q +  +    +L    +T+ + +  F + +R+ ++AL VYDAS        A+ M I  E+DI+YT+FKD     H PKFILF D E+ S++LSIRGT S+ DV+ DV+C D+ FLDGHAH+G+++G+ +++  +Q  L+  ++ HP ++++LTGHSLGAGTAEL+ + + L   +  +P  T+V+C+A+APPPVYR+  E+P ++ D+I+I I N D VPR+ LG+IA LL  +R++D L +       +L+     E   +L+ I+D + + +Q +FP L+HPG+I +  ++K      Q++  +    +++S+ F+  + LLE
Sbjct:   71 QDETGESVDFVLEKSLEKIKNIMFTKEMQEALDTISSSIEKLKQFD-LEAKQYGSPLFLLFAILKRVQADIMREQAENLADENETTFLPNQIFSM-KRIGNYALNVYDASWALTKHGIAKTMLI-EEEDILYTWFKDESQEAHCPKFILFKDDELESIILSIRGTFSIKDVVTDVVCVDQPFLDGHAHKGLVKGALQIITLVQESLKSAVQNHPGFKVVLTGHSLGAGTAELIYLELTLGASSNILPPGTKVECLALAPPPVYRSSTELPDKVQDSIKIIIFNNDLVPRLSLGNIANLLVKLRSLDDLNIPIISLISILINKISKEEEEHLQGIRDTLEKAQQTQFPHLQHPGRILHFKRIK------QQIRPR----SEDSSIFSTTILLLE 450          

BLAST of conserved unknown protein vs. nr
Match: gi|298705460|emb|CBJ28735.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 137.117 bits (344), Expect = 3.209e-31
Identity = 68/177 (38.42%), Postives = 105/177 (59.32%), Query Frame = 0

Query:  314 HVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVLV 490
            H+P ++L +DH   S+VLSIRGT S+ D + D++CD  +F+ G  HRG+ +G+  +L   ++ + Q L  H  YRL++TGHSLG G + LLTM++L+ K+E     T V C A APPPV+    ++ +E   AIR ++   D V RM L S  +L   ++ V+ +      +   LV
Sbjct:   64 HMPAYVLTVDHATESVVLSIRGTFSMQDTVTDLVCDSADFMGGSCHRGLRQGAEMLLADAKSDVLQQLNRHRGYRLVVTGHSLGGGVSILLTMMLLRRKSELGLGSTRVLCYAFAPPPVFGPLDKLSRETKRAIRSFVFGNDMVCRMSLASAYELFRDLKEVEGIGASLPQRLGYLV 240          

BLAST of conserved unknown protein vs. nr
Match: gi|546321907|ref|XP_005717681.1| (unnamed protein product [Chondrus crispus] >gi|507114239|emb|CDF37810.1| unnamed protein product [Chondrus crispus])

HSP 1 Score: 133.65 bits (335), Expect = 2.557e-29
Identity = 90/301 (29.90%), Postives = 145/301 (48.17%), Query Frame = 0

Query:  206 EAFDFVTNIIKNLRDFGGIDDEEFDSPLFVLLALIKTAQDEFDQAWINSLPKTSSVDSITFDLHRRVSSHALQVYDAS-----GEEDPKKQ-----------AQIMKIPSEKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVLV 490
            +A   V  I++   D  G+        +F+  +L++   D   ++ + +  + S   +    L R    +AL  Y        G  DP              AQ M       I+ +     G   P+  + +D    S+V++IRGT+S++D+I D++CD+E F  G+AH G+ R +  +   I   LR  L ++P Y + +TGHSLGAG A LLT V+L N        T+V+C AIAP PV+   H V  E +DA+  ++++ D V ++CL S   L         LEME+ D+ ++ V
Sbjct:  177 DAIQAVGKIVETFSD--GLRGPHSSKHIFLDTSLLRILHDSCSESHLITATRKSPSQTDVLVLGRYYMDYALSTYGFMLLKLLGVMDPSFNFMTHGTRGVDVAQYMLRLRPDQILVSKLDGEGIRAPRHFVAVDEREKSIVVAIRGTNSISDIITDLLCDNEPFAGGYAHGGMKRAAESLYTAILPTLRSALAKYPSYIIAVTGHSLGAGVAILLTKVLLMNGF------TDVKCYAIAPCPVFGPMHRVDSEWSDALECFVHHHDLVAKLCLASARNL--------ALEMERIDELQLTV 461          

BLAST of conserved unknown protein vs. nr
Match: gi|1160567763|ref|XP_020427560.1| (hypothetical protein PPL_11506 [Heterostelium album PN500] >gi|281201212|gb|EFA75426.1| hypothetical protein PPL_11506 [Heterostelium album PN500])

HSP 1 Score: 124.405 bits (311), Expect = 3.933e-26
Identity = 84/292 (28.77%), Postives = 147/292 (50.34%), Query Frame = 0

Query:  299 SEKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVL-----VRYDDDETANNLKRIQDEIMRIKQDRFPFLEH----PGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDNC 581
            S++D++   F  T +  P   + +DH   S+V+SIRGT    DV+ D++  +  FLDG+AH GI+R +     ++  +L + L++H  Y+LI+TGHSLGAGTA L T+ +  +K   IP    + C A APP V   +  + K  ++ I  ++ N D +PR+   S+  L  +V ++  LE   +  F++L     +  D     +N   +  E+    +++F   E     PG I  +YK+           +K  ++ + + S   E+ +   ++ DHL   Y  +  +C
Sbjct:  307 SKEDVISYRFTST-NFDPAHFISVDHSTESIVMSIRGTFHARDVLTDLVATNTPFLDGYAHTGILRSAQNKFNELSPLLLEQLKKHKGYKLIVTGHSLGAGTAALFTL-LFNSKYPEIP----IHCYAFAPPCVTSLEIALSKNCSNLITSFVLNNDIIPRLSYQSLEHLKQLVCSI--LENNSSRVFQILTAGNALGEDLTNKISNFFNLTREVKIDFKNKFSLSETTMLPPGDIYRIYKITS--------FDKNYIMERSNPSLFGEIIISGSLLTDHLPDNYENSLTSC 582          

BLAST of conserved unknown protein vs. nr
Match: gi|470245028|ref|XP_004356246.1| (hypothetical protein DFA_08761 [Cavenderia fasciculata] >gi|328869384|gb|EGG17762.1| hypothetical protein DFA_08761 [Cavenderia fasciculata])

HSP 1 Score: 120.553 bits (301), Expect = 7.181e-25
Identity = 85/292 (29.11%), Postives = 142/292 (48.63%), Query Frame = 0

Query:  301 KDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRA-VDKLEMEKTDQFKVLVRYDD--DETANNLK---RIQDEIMRIKQDRFPFLEH-----PGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDNC 581
            +DI+   F  T +  P   + +DH   S+V SIRGT    DV+ D++  +  FLDG AH GI+R +   L ++ +++ +T E+HP Y++I+ GHSLG GTA L T++      E  P+   + C A APP V   +  + ++  D I  +I N D +PR+   S+  L A++   +D         F +L   ++  DE  N +    ++  E+ ++     P +       PG I  +YKL            K  ++   + S   E+ +   ++ DHL   Y  AF++C
Sbjct:  358 EDIISYRFTST-NFDPGHYIAIDHNHESIVFSIRGTFHPRDVLTDLVATNAPFLDGFAHTGILRSAQNKLNELGSLIAETSEKHPTYKVIVVGHSLGGGTACLFTLLF----NEAYPK-VPIHCYAFAPPCVTSLEIALSRKAKDLITTFILNNDIIPRLSYQSLDHLKALIHTMLDNNTNFVQRSFHILTAGNNLGDELTNKIAGYLKVSREV-KVDYSNRPTISTTTMLPPGSIYRMYKL--------STMHKDYIMELSNPSLFNEIIISGTILSDHLPDNYEMAFNSC 634          

BLAST of conserved unknown protein vs. nr
Match: gi|831775986|ref|XP_012754665.1| (hypothetical protein SAMD00019534_057200 [Acytostelium subglobosum LB1] >gi|735855297|dbj|GAM22545.1| hypothetical protein SAMD00019534_057200 [Acytostelium subglobosum LB1])

HSP 1 Score: 118.242 bits (295), Expect = 3.777e-24
Identity = 84/292 (28.77%), Postives = 143/292 (48.97%), Query Frame = 0

Query:  301 KDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKT---DQFKVLVRYDD--DETANNLK---RIQDEIMRIKQDRF---PFLEHPGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDNC 581
            KD +  Y   + +  P   + +DH+  S++LSIRGT    D++ D++  + +FL G+AH GI+R +     ++ ++L Q L+EHP YRL+  GHSLGAGTA L T++     AEY   D  + C A APP V   +  + +     I  ++ N D +PR+   S+  L  MV ++  LE         F++L   +   +E  N +    R+  E+  +          +  PG I  +YK+      +Q+  +K  V+ + + +   E+ +   ++ DH    Y  A  +C
Sbjct:  275 KDDIICYRFTSTNFDPGHFVAIDHKTKSIILSIRGTFHARDILTDLVATNTKFLKGYAHNGILRCARNKFNELSSLLVQQLDEHPGYRLVPVGHSLGAGTAALFTLLF---HAEY--PDLPIHCYAFAPPCVTSFEMALDESTKRLITSFVLNDDMIPRLSYQSLEHLKTMVCSL--LESNTNLIQKSFQILAAGNTLGEEMTNKIAGFLRVSREVNIVATKSILSESTMLPPGDIYRIYKM------EQR--DKHHVMERSNPALFGEIIISSTLLTDHFPDNYENALSSC 551          

BLAST of conserved unknown protein vs. nr
Match: gi|66816363|ref|XP_642191.1| (hypothetical protein DDB_G0278621 [Dictyostelium discoideum AX4] >gi|60470503|gb|EAL68483.1| hypothetical protein DDB_G0278621 [Dictyostelium discoideum AX4])

HSP 1 Score: 117.472 bits (293), Expect = 9.636e-24
Identity = 83/296 (28.04%), Postives = 146/296 (49.32%), Query Frame = 0

Query:  299 SEKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQ-FKVLVRY--DDDETANNLKRIQDEIMRIKQD-RFPFLE---------HPGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDNC 581
            +++DI+ T +  T +  P   L  DH+  S++ SIRGT    D++ D++ +   FLDG AH G++R + K    +  ++ ++L+++ +Y LI+ GHSLGAG A L T ++ KN    IP    + C + A P V  ++  +  E    I  ++ N D VPR+C  S+  L  +V ++    +E+ D  F ++ +     ++    L    D  ++ K+D +  + E          PGK+  +Y  KP             V+ + + SF +E+ +   +I DH+   Y  AF  C
Sbjct:  392 AKEDIILTKWVST-NFDPGHYLCFDHKNKSVIFSIRGTFGARDILTDLVANQTSFLDGKAHTGMLRCAQKKFDDVVPIILESLQKYDKYSLIVVGHSLGAGVASLFT-ILFKNTFPDIP----IHCYSFATPCVTSSELALSIEYRPLIDTFVFNDDIVPRLCYASLEHLKTLVCSI----LEQNDSVFNLIFQTISAGNQLGEGLTNKMDSFLKYKRDIKLKYEELKLTGDSMLPPGKVYRIY--KPDNVN--------YVMEESNPSFFKEIIISNTLISDHMPDKYELAFKYC 667          

BLAST of conserved unknown protein vs. nr
Match: gi|1325284733|ref|XP_023327069.1| (uncharacterized protein LOC111700414 isoform X1 [Eurytemora affinis])

HSP 1 Score: 112.079 bits (279), Expect = 2.652e-23
Identity = 79/276 (28.62%), Postives = 140/276 (50.72%), Query Frame = 0

Query:  232 PLFVLLALIKTAQDEFDQAWINS----------LPKTSSVDSITFDLHRRVSSHALQVYDASGEEDPKKQAQIMKIPSEKDIVYTYFKDTG---DHVPKFILFLDHEIGSLVLSIRGTH-----SLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVL 489
            P+ + L L+   Q   D +W ++            K  +V    ++   ++S   L  ++ S EE   K  + +   +E ++V  + KD     D  P   L + H+   +VL+I GT      S+ D+++D+ CD  E+L+G AH GI   +  VLK  Q  L  T   +P Y LI+ G+SLGAG + L+++ +L+N    +P   ++ CIA   PPVYR+ + +     +   + ++N D +P + L ++ KL   +RA+DKL++++     +L
Sbjct:   81 PVLLTLGLMYKLQQMLDDSWFDTDQVFVKEEEKRDKLINVFGFYWNYLFKIS-WVLATHEKSVEEHQTKILESVN-SNEIEMVLFHVKDATTLEDQAPDTALIIHHDRQEIVLTICGTKITPAPSMGDIMLDLHCDSIEYLEGKAHEGIAIAAENVLKIFQEDLINTARMYPGYSLIIIGYSLGAGVSHLVSINLLQNN--LLPSSVKLYCIAYGAPPVYRSSN-ISYSHPNIFNV-LHNTDGLPTLSLHNVTKLFLQIRAIDKLKLKRRKILSLL 350          

BLAST of conserved unknown protein vs. nr
Match: gi|330796070|ref|XP_003286092.1| (hypothetical protein DICPUDRAFT_97274 [Dictyostelium purpureum] >gi|325083911|gb|EGC37351.1| hypothetical protein DICPUDRAFT_97274 [Dictyostelium purpureum])

HSP 1 Score: 115.161 bits (287), Expect = 4.631e-23
Identity = 85/288 (29.51%), Postives = 140/288 (48.61%), Query Frame = 0

Query:  299 SEKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCL----GSIAKL-LAMVRAVDKLEMEKTDQFKVLVRYDDDETANNLKRIQDEIMRIKQDRFPFLEHPGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDNC 581
            S++DI+ T +  T      FI F DH+  S+VLSIRGT S  DV+ D++ +   FLDG AH G++R + K   ++  ++ + L++H  Y +I+ GHSLGAG A L + ++ KN    IP    + C + A P V  ++  +  E    I  ++ N D VP   +     S+  L L  + A ++L    T + +  ++Y  D     +K   +E+    Q   P    PGK+  +YK   S            V+ + + SF +E+ +   ++ DH+   Y   F  C
Sbjct:  309 SKEDIILTKWTSTNYDPGHFICF-DHKNKSVVLSIRGTFSARDVLTDLVANQTPFLDGKAHTGMLRCAQKKFSELTPIILENLKKHEGYGVIIVGHSLGAGVASLFS-ILFKNNYPEIP----IHCYSFATPCVTSSEIALSIEYRSLIDTFVFNDDIVPLCSILEQNDSVFNLILQTISAGNQLGDGLTQKMESFLKYKRD-----IKLKYEELKLTDQSMLP----PGKVYRIYKPGESIN---------YVMEESNPSFFKEIIISNTLLSDHMPDKYEMGFQLC 572          

BLAST of conserved unknown protein vs. nr
Match: gi|1013939559|gb|KYQ93248.1| (hypothetical protein DLAC_11642 [Tieghemostelium lacteum])

HSP 1 Score: 114.005 bits (284), Expect = 4.726e-23
Identity = 83/299 (27.76%), Postives = 145/299 (48.49%), Query Frame = 0

Query:  301 KDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVL-----RQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQ-----FKVLVRYDDDETANNLKRIQDEIMRIKQD---RFPFLEH-----PGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDNC 581
            +DI+ T +  + +  P   +  DH   S+VL+IRGT    DV+ D++  +  F DG AH GI++ +    +++  +L     +Q   +   Y +++ GHSLGAG A L TM I   K   IP    + C A APP +  T+  + ++  + I  ++ N D VPR+C  S+  LL +V  +    ++K D      F++L      E   N  +++ +    +Q    + P L H     PGK+  +YK +         ++   V+ + + SF +E+ +   ++ DH+   Y  AF +C
Sbjct:  228 EDIILTKWT-SSNFDPGHYIAYDHARNSIVLAIRGTFHARDVLSDLVAKEAPFQDGFAHAGILKCAENKFQELGHILYLHYQKQMNLQQKSYTIVVCGHSLGAGVAALFTM-IFHQKYPQIP----IHCYAYAPPSLTSTEVSLNRKNRELIDTFVFNDDIVPRLCYASMEHLLELVLCI----LKKNDSVAQMGFQLLPNGQVTEKLGNFLKLKKDTETTRQSITFKEPQLSHRTMLPPGKVFRIYKPEK--------NDSYYVMEESNPSFFKEIIISGTLLTDHMPDKYEMAFKSC 508