BLAST of alanyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000005203 (supercontig:LSalAtl2s:LSalAtl2s274:26570:29820:-1 gene:EMLSAG00000005203 transcript:EMLSAT00000005203 description:"maker-LSalAtl2s274-augustus-gene-0.21")

HSP 1 Score: 504.212 bits (1297), Expect = 3.483e-164
Identity = 323/847 (38.13%), Postives = 476/847 (56.20%), Query Frame = 0

Query:   66 NSALDAEE--------YFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGA-----DKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVF--------EEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSP--DEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLID------DAAKVL------DCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQ-VGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEG-----------------------QRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNAN---ASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARAT-GAQALFGEKYDDVVRVVAMG---------P-------NSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKC----RELEKQSAALQSKLASASIDDYLQQVEVINEVNVL 829
            +S+L A+E        +  +++H    SSS +P +DPTLLF NAGM QFK VF+G      DK    RA  SQ+CVRAGGKHNDLE+VG  + HHTFFEMLGN+SFGDYFK+E  +++W+ LTK+  + E++L+V+ F        + D + ++IW D +GVP+ER++    KDNFW+MGDTGPCGPC+EI +D      GG   +   ++D    +EIWNLVF+Q++R +D +L  LPK  +D G+GLER+ + +Q   SNY+ D+F  + +       A+K        +  D+ + + RV+ADHIR+ + +I DG  P N GRGYVLRRI+RR IR   + +  K  F   LV+ +V  +G  +PEL K    I+ ++  E  +FL+TL  G  LLD  I  +DS    KI+ GDV ++LYDTYGFP+DLT  +  ER +++D+ G+E   E                        Q  + +S S+  D  K +    ++     Y  S   A ++AL      V+ I+ G +  I+L+ T FY E GGQ+ D+G +V  N     F+V NVQ   G V  H GTL +G + IG+ V   I+T  R+ +  NH+ TH+L+ AL  VL     QKGSLV +D+LRFDF+  + +T  ++ A+E  VN  +R N E+      +  A+   G +A+F E Y D VRVV++G         P        S+E CGGTH+ R G++ +F ++SE  +A G+RRI ALTG  A  K   QK +L          + N +D     +Q+     +E+ K    L   ++S++I  + ++ E+ N +  L
Sbjct:    2 DSSLKADEIRKLFLDFFSKKKDHTYYHSSSTIPLDDPTLLFINAGMNQFKSVFVGTIDPNNDKAKLVRAVNSQKCVRAGGKHNDLEDVGKDSYHHTFFEMLGNWSFGDYFKKEICEWSWEFLTKELKMPEDRLYVSYFGGNEKTGLDPDLDCKQIWLD-LGVPEERLLPGNMKDNFWEMGDTGPCGPCSEIHFDR----VGGRNAAHLVNQDDPMVLEIWNLVFIQFNREADKSLKLLPKKHIDCGLGLERLVSAIQNKSSNYDTDIFTPIFEAIHERSGASKPYQGKFGDEDPDQIDMAYRVVADHIRTLTIVIADGGRPDNVGRGYVLRRILRRGIRFATEKLNAKPGFFGSLVDVVVSVLGHIFPELTKDPELIKDVINEEEIQFLKTLNRGRKLLDRTIGKMDS----KILPGDVAWRLYDTYGFPIDLTQLMVEERNMTVDLEGYEKAKEKAVLMSQGKGAGTEDRLALDVHSIQELQDKSISTTDDKFKYHYEEKSSDFDSDYEFSGIEATIMALRYEKKFVDSIACGTECGIILDHTSFYAEQGGQIYDEGFIVKVNDDSVEFKVKNVQVRAGYVL-HVGTLGEGELKIGEKVFLQIDTIRRQNVMKNHTGTHILNFALRQVLEGDSDQKGSLVASDKLRFDFTCKKAMTTDQVKAVEMCVNEIIRKNEEIFAKEASLAVAKTIQGLRAVFDETYPDPVRVVSVGIRVEKMEADPTGAAGSVTSVEFCGGTHLKRAGHLQVFVISSEEAIAKGIRRIIALTGPEA--KKATQKAVL----------LQNKVDKVSLVVQEAKLSSKEMVKLITNLSEDISSSTISQW-KKDEMRNSLKAL 825          

BLAST of alanyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000005940 (supercontig:LSalAtl2s:LSalAtl2s320:546129:548925:-1 gene:EMLSAG00000005940 transcript:EMLSAT00000005940 description:"maker-LSalAtl2s320-augustus-gene-4.11")

HSP 1 Score: 267.314 bits (682), Expect = 1.394e-76
Identity = 211/704 (29.97%), Postives = 325/704 (46.16%), Query Frame = 0

Query:  152 MLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSL--------RVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIR---HGYQVGCKVPFMHLLVETLVQEMGEAYPE-----LAKQKGHIEQILQRENERFLETLELGLALLDSHIDSI----------------------DSAK----------SDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNK-------KINVAHLNATEF--------LGYGKSQASAEVVALY-----KGDT-AVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNA--NASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFE-PITDKEILAIEQYVNREVRANYEVTTTLMPIEEAR-----------ATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHV 772
            MLG++SF DYFK++AIQFAW+ LT    + +E L VT F++D E  + W    G+  +++I  G  DNFW+MG TGPCGPCTEI Y                   +  EIWNLVFM+Y R  +G L  L K  VDTGMGLER+ ++L    SNY+ DLF+ + D  +K L   D  + S         R++ADH R  +  + DG+LP      Y LR IIRR ++     +    +V F+  L+  + + +G+ + E       K +  +  +L+ E++   E  +   +LL+   D++                      D+ K            KII+G+  FKLYD+ GF  +     A       D   +E  +   + K +S  +   N+       KI +A      F          Y      + ++ L      K D+  V +++ GD  I++  ++PFY E+GGQ    G++  +   + F V +V    G V+ H GT+  G+    D V+  I+   R     NH+ +HLL+AA+N VL     Q+   V+    +F    F+  ++  EI  IE+  N       ++     PI +A+            +    L GEKY D V ++ + P   E C GTHV  T ++   T+T    V+SG++    LTGQ A+D        LD+V
Sbjct:    1 MLGSWSFNDYFKKDAIQFAWEFLTGPLEIPKEFLNVTYFKDDLETREYWLSQ-GLSPDKIIPKGETDNFWEMGSTGPCGPCTEIHY-------------------KDTEIWNLVFMEYVRRPNGELASLDKKHVDTGMGLERLTSILNGYTSNYDTDLFIPIFDKISK-LSGADPYSGSFDKLQDINYRILADHSRMIATCLSDGLLPD---VSYRLRNIIRRCMKISAESFNSKNQVDFLKELIYCVSESLGDHHREYLESPFLKDEAKLSLLLKFEHDVLKELEKTHKSLLNKLKDAVPFKTLFEKYESELDPSMIGRFYDAFKMYSCNALNNAEPKIISGEFAFKLYDSIGFKSEDIELFALLLDAKYD--PYEFHLYSAKVKEQSKVTTSVNRTNMEILSKITLAPKTDDSFKYKWVQSGFKYQFYIIESNILQLIQISNDKSDSQCVGKLNRGDTGILICEKSPFYSEAGGQAXXTGSITGSLGESVFHVHDVFSLKGVVY-HVGTVHSGTFMRQDLVKLKIDPFKRVTHMQNHTGSHLLNAAMNTVLP-FTTQRSLRVDXTGFKFXACIFKLNLSSSEINDIEEKAN-------QMINNADPIVQAKDKYLDSFIKNSKSKVLTLPGEKYPDKVSMICL-PQFSEPCCGTHVNCTKDVEALTITHIKSVSSGIKSFRCLTGQKAIDARENGLAFLDNV 668          

BLAST of alanyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000000400 (supercontig:LSalAtl2s:LSalAtl2s1063:84148:84558:-1 gene:EMLSAG00000000400 transcript:EMLSAT00000000400 description:"augustus_masked-LSalAtl2s1063-processed-gene-0.3")

HSP 1 Score: 63.929 bits (154), Expect = 2.336e-12
Identity = 27/62 (43.55%), Postives = 41/62 (66.13%), Query Frame = 0

Query:    3 LASSSDWQLVNGK--LHFEKRFANFTDAFGFMTKVAIVSEKMNHHPEWSNIYATVIIDLITH 62
            L +   W++V  +  ++ E  F +F +AFGFMT+VA+ ++ MNHHPEW N+Y  V + L TH
Sbjct:   56 LLTKKSWKMVENRDAIYKEFLFKDFNEAFGFMTRVALKADAMNHHPEWFNVYNKVQVTLSTH 117          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|123555227|sp|Q31F91.1|SYA_THICR (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 949.888 bits (2454), Expect = 0.000e+0
Identity = 476/808 (58.91%), Postives = 585/808 (72.40%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQAVAENVAKSHAQGHGMIVVLSAMSGETNRLL 880
            ++F +Q H  V SS +VP NDPTLLFTNAGMVQFK+ FLG + R+Y RAT+ QRC+RAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK EAIQ+AW  LT++ GL EEKLW+TVFEED EAE IW   +GV  ER  R GAKDNFW MGDTGPCGPC+EIFYDHG DVAGGPPGSPDEDGDRYIEIWNLVFMQ+DRS DGTLTPLPKP VDTGMGLER+AAV+Q  H+NY+IDLF  ++  A+++    D +N SLRVIADHIRS +F+++DGVLPSNEGRGYVLRRIIRRAIRHGY++G    F + LV TLV++MGEAYPEL K++ ++E+ L+ E ERF ETLE G+ +L+  I  +    S  +I+G   FKLYDTYGFP+DLT DVARER LS+D  GFE +ME QRA+ARSAS+FG   K  V +  +T F+GY + +A AE+ A++  + +VE+ S G +AI++LNQTPFY ESGGQV D+G+L     SF V N QK  G  F H G +  GSIS+G  + A I+  +R     NHSATHLLHAAL  VLG HV QKGSLV+ +RLRFDFSHFEPI+ +++L IEQ VN  +  N +V    M IE A+A GA ALFGEKY DVVRVV MG  SIELCGGTHV  TG IG F +TSESG+ASGVRRIEA+TG+ A    +  +  L ++A+ LK+          + + + +ELEKQ   LQSKLAS+  DD    V  +N VNVL  ++   D   L  T ++++   E  A          +VL+A+ G+   L+
Sbjct:   12 DFFVKQGHTAVHSSPVVPGNDPTLLFTNAGMVQFKETFLGQETREYKRATSVQRCIRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWTFLTEELGLPEEKLWITVFEEDREAEDIWLKEMGVSAERFSRCGAKDNFWSMGDTGPCGPCSEIFYDHGADVAGGPPGSPDEDGDRYIEIWNLVFMQFDRSEDGTLTPLPKPSVDTGMGLERLAAVMQNQHNNYDIDLFQAIVKKASELTGEKDLANSSLRVIADHIRSCAFMVVDGVLPSNEGRGYVLRRIIRRAIRHGYKLGQTDIFFYQLVPTLVEQMGEAYPELVKEQANVERALKLEEERFAETLENGMKILEEDIAQL----SGSVISGSTAFKLYDTYGFPLDLTADVARERNLSVDEAGFEQEMEAQRARARSASNFGAQSKNKVDYTGSTHFIGYEQDEAEAEIAAIFVDNVSVEKASEGQEAIVILNQTPFYAESGGQVGDQGSLTEGMNSFHVDNCQK-QGAAFLHIGKVTAGSISVGQTILAHIDVKARRASERNHSATHLLHAALRTVLGTHVGQKGSLVQPERLRFDFSHFEPISAEQLLEIEQLVNHNIMLNAQVMMEEMDIEAAKAKGAMALFGEKYGDVVRVVDMGDFSIELCGGTHVNWTGEIGPFRITSESGIASGVRRIEAVTGEAAWQTIYDMEKSLLNIAANLKTDKPQVESKVAQLVTEQKELEKQLKQLQSKLASSQGDDLASSVTEVNGVNVLAAELEGADVNTLRETLDKLRDKLEPAA----------IVLAAVDGDKVSLV 804          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|83305855|sp|Q4K843.1|SYA_PSEF5 (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 932.939 bits (2410), Expect = 0.000e+0
Identity = 461/786 (58.65%), Postives = 564/786 (71.76%), Query Frame = 0

Query:   74 YFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPN-SIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQ 849
            +F EQ H  V+SSSL+P NDPTLLFTNAGM QFKD FLG +KR Y RA +SQ+CVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI FAW  LT  K   L +EKLWVTV+  DDEA  IWT  +GVP ER++RIG       A DNFW MGDTGPCGPCTEIFYDHG D+ GGPPGSP+EDGDRYIEIWN VFMQ++R++DG L PLP P VDTGMGLERI+AVLQ VHSNYEIDLF  L+D AAK + CT+ +  SL+V+ADHIRS  FLI DGVLPSNEGRGYVLRRIIRRA RHG ++G K  F + +V  LV EMGEA+PEL  Q+ HIE++L+ E E+F +TLE GL +L+  +  +  +    ++ GDV+FKLYDTYGFP+DLT D+ARER L+LD  GFE +ME QR +ARSASSFG +    V    ATEF GY  +  SA+VVALYK   +V+ +S G++ ++VL+QTPFY ESGGQ+ D G L   NA F V +  K  G  F H+G L KGS+++G  VE  ++   R   + NHSATHLLHAAL  VLG+HVQQKGSLV++ RLRFDFSHFE I  ++I A+E  VN E+R N  V T    I+ A+  GA ALFGEKY D VRV++MG + S+ELCGG H  RTG+IGL  +TSE GVASGVRRIEA+TG  A+   +  +  L   A+ +K S  N +D     +++ R LEKQ   LQ+K ASA+ DD   Q   +  V VL  ++   D K LL   ++++
Sbjct:   13 FFEEQGHTRVASSSLIPGNDPTLLFTNAGMNQFKDCFLGQEKRAYTRAVSSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAITFAWTFLTSDKWLNLPKEKLWVTVYATDDEAYDIWTKEVGVPAERMVRIGDNKGAPYASDNFWTMGDTGPCGPCTEIFYDHGADIWGGPPGSPEEDGDRYIEIWNNVFMQFNRTADGVLHPLPAPSVDTGMGLERISAVLQHVHSNYEIDLFQSLLDAAAKAIGCTNDAQASLKVVADHIRSCGFLIADGVLPSNEGRGYVLRRIIRRACRHGNKLGAKGSFFYQIVAALVAEMGEAFPELKSQQAHIERVLKAEEEQFAKTLEQGLKILEQDLAELKGS----VVPGDVVFKLYDTYGFPMDLTGDIARERNLTLDEEGFEREMEAQRVRARSASSFGMDYNSLVKVDVATEFTGYHATSGSAKVVALYKEGQSVDMLSEGEEGVVVLDQTPFYAESGGQIGDCGYLQAGNARFDVRDTTKTGG-AFLHHGVLAKGSLTVGAPVETQVDAQVRHATSLNHSATHLLHAALRQVLGEHVQQKGSLVDSQRLRFDFSHFEAIKPEQIKALEDIVNAEIRKNTPVETEETDIDTAKKKGAMALFGEKYGDSVRVLSMGGDFSVELCGGIHANRTGDIGLLKITSEGGVASGVRRIEAVTGAAALAYLNAAEEQLKEAATLVKGSRDNLIDKLSAVLERNRLLEKQLEQLQAKAASAAGDDLSAQAADVKGVKVLAARLDGQDGKALLALVDQLK 793          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830246|sp|A8GA09.2|SYA_SERP5 (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 930.243 bits (2403), Expect = 0.000e+0
Identity = 456/778 (58.61%), Postives = 558/778 (71.72%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKK--FGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVPDNDPTLLFTNAGM QFKDVFLG DKR Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI +AW+LLT +  F L +E+LWVTV+E DDEA  IW   IGVP ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWNLVFMQ++R SDGT+ PLPKP VDTGMGLERIAAVLQ V+SNYEIDLF KLI   AKV   TD  NKSLRVIADHIRS +FL+ DGV PSNEGRGYVLRRIIRRA+RHG  +G K  F + LV  L++ MG A  EL +Q+  +EQ+L+ E ++F  TLE GL LLD  + ++        ++G+  F+LYDTYGFPVDLT DV RERGL +D  GFE  ME QR +AR +S FG +    +    A++F GY   Q  + V AL++    V  I AG++A++VL++TPFYGESGGQV DKG L  ANA F+VS+ QK  GQ   H G L  GS+ + D V+A I+T  R  I  NHSATHLLHAAL   LG+HV QKGSLV    LRFDFSHFE +  ++I  +E  VN+++R N  V T +M +++A+  GA ALFGEKYDD VRV+ MG  S ELCGGTH +RTG+IGLF + SESG A+G+RRIEA+TG  A+   HQQ  LL  VA  +K    N  D     + + R LEK+   L+ + A+        + +++N V +L  ++  V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPDNDPTLLFTNAGMNQFKDVFLGLDKRAYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAISYAWELLTGENWFNLPKERLWVTVYETDDEAFDIWQQQIGVPAERIIRIGDNKGGAFASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNLVFMQFNRQSDGTMLPLPKPSVDTGMGLERIAAVLQHVNSNYEIDLFSKLIAAVAKVTGATDLDNKSLRVIADHIRSCAFLVSDGVTPSNEGRGYVLRRIIRRAVRHGNMLGAKDTFFYKLVAPLIEVMGPAADELKRQQSLVEQVLKTEEDQFARTLERGLTLLDEELANLQG----DTLDGETAFRLYDTYGFPVDLTADVCRERGLKVDEAGFEQAMEAQRRRARESSGFGADYNSMIRVDGASQFSGYDHEQQQSTVTALFRDGQPVNEIHAGEEAVVVLDETPFYGESGGQVGDKGVLKAANADFEVSDTQK-YGQAIGHQGKLSHGSLKVNDRVDAKIDTVRRNRIRLNHSATHLLHAALRQTLGEHVAQKGSLVNDKYLRFDFSHFEAMKPEQIRTVEDLVNQQIRRNLPVQTEVMALDDAKGKGAMALFGEKYDDNVRVLTMGDFSTELCGGTHASRTGDIGLFRILSESGTAAGIRRIEAVTGDGAIATLHQQNDLLQDVAHLVKGDSNNLTDKVRAVLDRTRALEKELQQLKDQQAAQESASLSSKAKMVNGVQLLVSQLDNVEAKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830024|sp|A8ANQ1.1|SYA_CITK8 (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 929.858 bits (2402), Expect = 0.000e+0
Identity = 456/778 (58.61%), Postives = 563/778 (72.37%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKK--FGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP+NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT +  F L +++LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNYEIDLF  LI+  AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  EL +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +    A+EF GY   + + +V AL+    AV+ ISAG +A++VL+QTPFY ESGGQV DKG L  A  +F VS+ QK  GQ   H GTL  GS+ +GDAV+A ++   R  I  NHSATHL+HAAL  +LG HV QKGSLV    LRFDFSH E +   EI A+E  VN ++R N  V T +M +E A+A GA ALFGEKYDD VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H +   L  +A  LK    N  +     +++ R+LEK+   L+ + A+    +   +   IN V +L  ++A V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTGENWFALPKDRLWVTVYETDDEAYEIWEKEVGIPRERIIRIGDNKGAAYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYEIDLFRTLIEAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVVPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEELKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDGASEFKGYDHLELNGKVTALFVDGKAVDAISAGQEAVVVLDQTPFYAESGGQVGDKGELKGAGFAFAVSDTQK-YGQAIGHIGTLSTGSLKVGDAVQADVDDARRARIRLNHSATHLMHAALRQILGTHVAQKGSLVNDKVLRFDFSHNEAMKPSEIRAVEDLVNAQIRRNLPVETNIMDLEAAKAKGAMALFGEKYDDRVRVLSMGDFSTELCGGTHASRTGDIGLFRIVSESGTAAGVRRIEAVTGEGAIATLHAESDRLSDIAQLLKGDSQNLGEKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAVDINGVKLLVSELAGVEPKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|44888327|sp|Q7UBU3.1|SYA_SHIFL (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS >gi|123146773|sp|Q0T1C3.1|SYA_SHIF8 RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 455/778 (58.48%), Postives = 561/778 (72.11%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT  K F L +E+LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNY+IDLF  LI   AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  +L +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +   +A+EF GY   + + +V AL+    AV+ I+AG +A++VL+QTPFY ESGGQV DKG L  AN SF V + QK  GQ   H G L  GS+ +GDAV+A I+   R  I  NHSATHL+HAAL  VLG HV QKGSLV    LRFDFSH E +  +EI A+E  VN ++R N  + T +M +E A+A GA ALFGEKYD+ VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H     L  VA  LK    N  D     +++ R+LEK+   L+ + A+    +   +   +N V +L  +++ V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQK-YGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|123147797|sp|Q0TEI3.1|SYA_ECOL5 (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 454/778 (58.35%), Postives = 561/778 (72.11%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT  K F L +E+LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNY+IDLF  LI   AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  +L +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +   +A+EF GY   + + +V AL+    AV+ I+AG +A++VL+QTPFY ESGGQV DKG L  AN SF V + QK  GQ   H G L  GS+ +GDAV+A ++   R  I  NHSATHL+HAAL  VLG HV QKGSLV    LRFDFSH E +  +EI A+E  VN ++R N  + T +M +E A+A GA ALFGEKYD+ VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H     L  VA  LK    N  D     +++ R+LEK+   L+ + A+    +   +   +N V +L  +++ V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQK-YGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSERLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|123561812|sp|Q32CN1.1|SYA_SHIDS (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 455/778 (58.48%), Postives = 561/778 (72.11%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT  K F L +E+LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNY+IDLF  LI   AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  +L +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +   +A+EF GY   + + +V AL+    AV+ I+AG DA++VL+QTPFY ESGGQV DKG L  AN SF V + QK  GQ   H G L  GS+ +GDAV+A ++   R  I  NHSATHL+HAAL  VLG HV QKGSLV    LRFDFSH E +  +EI A+E  VN ++R N  + T +M +E A+A GA ALFGEKYD+ VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H     L  VA  LK    N  D     +++ R+LEK+   L+ + A+    +   +   +N V +L  +++ V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQDAVVVLDQTPFYAESGGQVGDKGELKGANFSFVVEDTQK-YGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830083|sp|A8A3H4.1|SYA_ECOHS (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 924.85 bits (2389), Expect = 0.000e+0
Identity = 454/778 (58.35%), Postives = 561/778 (72.11%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT  K F L +E+LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNY+IDLF  LI   AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  +L +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +   +A+EF GY   + + +V AL+    AV+ I+AG +A++VL+QTPFY ESGGQV DKG L  AN SF V + QK  GQ   H G L  GS+ +GDAV+A ++   R  I  NHSATHL+HAAL  VLG HV QKGSLV    LRFDFSH E +  +EI A+E  VN ++R N  + T +M +E A+A GA ALFGEKYD+ VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H     L  VA  LK    N  D     +++ R+LEK+   L+ + A+    +   +   +N V +L  +++ V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQK-YGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|20532264|sp|Q9I553.1|SYA_PSEAE (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 924.465 bits (2388), Expect = 0.000e+0
Identity = 449/786 (57.12%), Postives = 569/786 (72.39%), Query Frame = 0

Query:   74 YFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPN-SIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQ 849
            +F E+ H  V+SSSL+P NDPTLLFTNAGM QFKD FLG +KR Y RATTSQ+CVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI +AW+ LT  K   L +EKLWVTV+  DDEA  IWT  +GVP ER++RIG       A DNFW MGDTGPCGPCTEIF+DHG ++ GGPPGSP+EDGDRYIEIWN VFMQ++R++DG + PLP P VDTGMGLER++AVLQ VHSNYEIDLF  L+  +A+ + C +    SL+V+ADHIRS SFLI DGVLPSNEGRGYVLRRIIRRA RHG ++G +  F H +V  LV EMG+A+PEL +Q+ HIE++L+ E E+F +TLE GL +L+  +  +  +    +I G+V+FKLYDTYGFPVDLTND+ARER L++D  GFE +ME QR +AR++S+FG +    V     T FLGY     + ++VAL++   AVER+  G++ ++VL+QTPFY ESGGQV D G L  A   F V +  K  G    H+G + +G++S+G AV+A ++   R+  A NHSATHLLHAAL  VLGDHVQQKGSLV++ RLRFDFSHFE I  +++ A+E  VN E+R N EV T    I+ A+A GA ALFGEKY D VRV++MG + S+ELCGGTHV+RTG+IGLF +TSE GVA+GVRRIEA+TG  A+   +  +  L   AS +K S  N LD     +++ R LEK+   L++K ASA+ DD       I    VL  ++  +D K LL   ++++
Sbjct:   13 FFEEKGHTRVASSSLIPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWEFLTGEKWLNLPKEKLWVTVYATDDEAYDIWTKEVGVPAERMVRIGDNKGAPYASDNFWAMGDTGPCGPCTEIFFDHGPEIWGGPPGSPEEDGDRYIEIWNNVFMQFNRTADGVMHPLPAPSVDTGMGLERVSAVLQHVHSNYEIDLFQNLLKASAEAIGCANDDAPSLKVVADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRACRHGNKLGARGTFFHKIVAALVAEMGDAFPELKQQQAHIERVLKTEEEQFAKTLEQGLKILEQDLAELQGS----VIPGNVVFKLYDTYGFPVDLTNDIARERELTIDEDGFEREMEAQRERARASSAFGMDYNSLVKVDGETRFLGYQGVSGAGQIVALFRDGQAVERLEEGEEGVVVLDQTPFYAESGGQVGDSGYLEAAGVRFDVRDTTKAGG-AHLHHGVVARGNLSVGGAVKAEVDASVRQATALNHSATHLLHAALRQVLGDHVQQKGSLVDSQRLRFDFSHFEAIKAEQLKALEDIVNAEIRRNTEVETEETDIDTAKAKGAMALFGEKYGDQVRVLSMGGDFSVELCGGTHVSRTGDIGLFKITSEGGVAAGVRRIEAVTGAAALAYLNGAEEQLKEAASLVKGSRDNLLDKLGALLERNRSLEKELEQLKAKAASAAGDDLSAAAVEIKGAKVLAARLDGLDGKALLALVDQLK 793          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|123558824|sp|Q31X62.1|SYA_SHIBS (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 924.465 bits (2388), Expect = 0.000e+0
Identity = 454/778 (58.35%), Postives = 562/778 (72.24%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT  K F L +E+LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNY+IDLF  LI   AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  +L +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +   +A+EF GY   + + +V AL+    AV+ I+AG +A++VL+QTPFY ESGGQV DKG L +AN SF V + QK  GQ   H G L  GS+ +GDAV+A ++   R  I  NHSATHL+HAAL  VLG HV QKGSLV    LRFDFSH E +  +EI A+E  VN ++R N  + T +M +E A+A GA ALFGEKYD+ VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H     L  VA  LK    N  D     +++ R+LEK+   L+ + A+    +   +   +N V +L  +++ V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKDANFSFAVEDTQK-YGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKML 786          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1011362591|ref|WP_062275569.1| (alanine--tRNA ligase [Marichromatium gracile] >gi|1003483950|gb|KXX64375.1| alanine--tRNA ligase [Marichromatium gracile])

HSP 1 Score: 967.222 bits (2499), Expect = 0.000e+0
Identity = 455/777 (58.56%), Postives = 576/777 (74.13%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQ 849
            +YF ++ H  VSSS LVP NDPTLLFTNAGMVQFKDVFLG ++R Y+RA T+QRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK EAI FAWQ LT + GL  E+LWVTV+E+DDEA  IW   +GV  ER  R GAKDNFW MGDTGPCGPC+EIFYDHG +VAGGPPGSPDEDGDRY+EIWNLVFMQ++R ++G +TPLP+P VDTGMGLER+AAVLQ VHSNY+IDLF  LI  AA+   C D  +KSLRVIADHIRS++FLI+DGV P NEGRGYVLRRI+RRAIRHGY++G   PF H LV  LV EMG AYPEL   + H+E+I+  E ERF ETLE G+ +L+   ++I     ++II G+ +FKLYDTYGFP DLT D+ARERGL +D+ GFE  M  Q+A+AR+AS FG  + + +     TEF GY + +  A VVALY+   + + + AG+  +++L+ TPFYGESGGQV D+G + ++   F+V++  K  G +  H G L KG + +GDAV A ++ + R  IA NHSATHLLHAAL  VLG+HVQQKGSLV  +RLRFDFSHFEP++ +++  IE+ VN ++RAN+ V T +M +E+A+A+GA ALFGEKY D VRV+ MG  S+ELCGGTHV   G+IGL  + SE+G+ASGVRRIEA+TG  A+D     ++ +  +A  LK +  +  +  E+ +++ R LEK+   L+SKLASA+  D   Q E I  V VL  ++   D K L  T ++++
Sbjct:   13 DYFAQRQHEQVSSSPLVPANDPTLLFTNAGMVQFKDVFLGRERRGYSRAVTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIGFAWQYLTGELGLPAERLWVTVYEDDDEAADIWLKEVGVSAERFSRCGAKDNFWSMGDTGPCGPCSEIFYDHGPEVAGGPPGSPDEDGDRYVEIWNLVFMQFNRDAEGNMTPLPRPSVDTGMGLERLAAVLQGVHSNYDIDLFRNLIAAAAEATGCEDLGDKSLRVIADHIRSSAFLIVDGVTPGNEGRGYVLRRIMRRAIRHGYRLGQSQPFFHRLVAALVAEMGAAYPELVAAQAHVERIVLLEEERFAETLEHGMRILE---EAIAGLAGERIIPGETVFKLYDTYGFPTDLTADIARERGLGVDLEGFEAAMADQKARARAASQFGGGQDVEIDLQGETEFRGYERCEDEATVVALYRDGESCDSLGAGERGMVILDLTPFYGESGGQVGDRGWIASSGGRFEVTDTLKKGGALVCHLGELRKGELHVGDAVRAEVDAERRRAIALNHSATHLLHAALRQVLGEHVQQKGSLVGPERLRFDFSHFEPVSREQLREIERLVNSQIRANHLVETRIMSLEDAKASGAMALFGEKYADQVRVLRMGEFSVELCGGTHVKAVGDIGLMKIVSEAGIASGVRRIEAVTGAGALDWVEADESRVLRIAGLLKGAREDVDERVEQLLERSRRLEKELEQLKSKLASAAGQDLAAQAEEIAGVRVLAAELEGADPKGLRETVDQLK 786          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1356473791|ref|WP_105213730.1| (alanine--tRNA ligase [Pseudoalteromonas sp. T1lg22])

HSP 1 Score: 962.6 bits (2487), Expect = 0.000e+0
Identity = 466/791 (58.91%), Postives = 585/791 (73.96%), Query Frame = 0

Query:   59 LITHKAKNSALDAEEYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQ 849
            L T + +   LD   YF +  H +V S+SLVP NDPTLLF NAGMV FKDVFLGA+KR Y RA ++QRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKE+AI FAW+ LT++  L +EKLWVTVF EDDEA  IW + IGVPKERV RIGAKDNFW MGDTGPCGPC+EIFYDHGE+V GGPPG+P+EDGDRYIEIWNLVFMQ++R +DGT+ PLP P +DTGMGLERIAA+LQ VHSNYEIDLF  LI  AAKV +  D  +KSLRVIADHIRS +FL++DGV+PSNEGRGYVLRRIIRRA+RHG ++G K  F H +V  LV +MG+AYPELA+++G IE++L+ E E+F +TLE GLA+L+ H+ S++      +I GDV+FKLYDTYGFP DLT DVARER L++D  GF+  M+ QR KA+ A  FG +    +    +TEF GY   Q SA VV ++     V  ++ G++ I++LN+TPFY ESGGQV D G L  AN  F V++ QK  G  FAH+G +  G I + D V+A I+ + R+ I  NH+ATHL+H AL   LG+HV QKGSLV+ADRLRFDFSHFE +T +++  +E+ VN E+R N+ + T LM I+ A+  GA ALFGEKYDD VRVV++G  SIELCGGTHV RTG+IGLF + SE+G+A+GVRRIEA+TG  A++    Q+  L  +A+ LK+   N LD  +  +++ + LEKQ A L  KLASA+    L   + IN V VL   +A  ++K L G  + ++
Sbjct:    4 LTTAQLRQKFLD---YFAKHQHQVVPSASLVPGNDPTLLFNNAGMVPFKDVFLGAEKRPYTRAASAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEQAIAFAWKFLTEELQLPKEKLWVTVFTEDDEAADIWINQIGVPKERVSRIGAKDNFWSMGDTGPCGPCSEIFYDHGEEVWGGPPGTPEEDGDRYIEIWNLVFMQFNRHADGTMEPLPNPSIDTGMGLERIAAILQGVHSNYEIDLFQALISSAAKVTNAQDMEDKSLRVIADHIRSCAFLVLDGVMPSNEGRGYVLRRIIRRAVRHGNKLGAKGTFFHKIVAELVAQMGDAYPELAQKQGIIEKVLRIEEEQFGKTLERGLAILEEHLSSLEG----DVIPGDVVFKLYDTYGFPADLTADVARERFLTIDEKGFQECMDVQRKKAQQAGKFGADYNNQLRSDKSTEFKGYDAQQHSATVVEMFAAGEKVSLLNDGEEGIVILNRTPFYAESGGQVGDTGVLATANGQFIVTDTQKL-GNAFAHHGKV-VGQIGVNDKVDARIDAERRDRIKKNHTATHLIHEALRQELGEHVNQKGSLVQADRLRFDFSHFEGVTPEQLQHVEKKVNDEIRHNFALQTELMAIDAAKEKGAMALFGEKYDDEVRVVSIGDYSIELCGGTHVERTGDIGLFKIVSEAGIAAGVRRIEAVTGADALNHIAAQEEKLTQIAALLKADSGNVLDKVQGLLERSKGLEKQVAQLNDKLASAAGASLLDGAKDINGVKVLIANVAGTESKALRGMVDDLK 785          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1356008029|ref|WP_105190615.1| (alanine--tRNA ligase [Pseudoalteromonas sp. T1lg48])

HSP 1 Score: 962.214 bits (2486), Expect = 0.000e+0
Identity = 468/791 (59.17%), Postives = 583/791 (73.70%), Query Frame = 0

Query:   59 LITHKAKNSALDAEEYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQ 849
            L T + +   LD   YF +  H +V S+SLVP NDPTLLF NAGMV FKDVFLGA+KR Y RA ++QRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKE+AI FAW+ LT++  L +EKLWVTVF EDDEA  IW + IGVPKERV RIGAKDNFW MGDTGPCGPC+EIFYDHGE+V GGPPG+P+EDGDRYIEIWNLVFMQ++R +DGT+ PLP P +DTGMGLERIAA+LQ VHSNYEIDLF  LI  AA+V +  D  +KSLRVIADHIRS +FL++DGV+PSNEGRGYVLRRIIRRA+RHG ++G K  F H LV  LV +MG+AYPELAK++  IE++L+ E E+F +TLE GLA+L+ H+ S++      +I GDV+FKLYDTYGFP DLT DVARER L++D  GF+  M+ QR KA+ A  FG +    +    +TEF GY   Q SA VV L+ G   V  ++ G + I++LN+TPFY ESGGQV D G L  AN  F V++ QK  G  FAH+G +  G I + D V+A I+ + R+ I  NH+ATHL+H AL   LG+HV QKGSLV+ DRLRFDFSHFE +T +++  IE+ VN E+R N+ + T LM I+ A+  GA ALFGEKYDD VRVV++G  SIELCGGTHV RTG+IGLF + SE+G+A+GVRRIEA+TG  A++    Q+  L  +A+ LK+  AN LD  +  +++ + LEKQ   L  KLASA+    L   + IN V VL   +A  ++K L G  + ++
Sbjct:    4 LTTAQLRQKFLD---YFAKHQHQVVPSASLVPGNDPTLLFNNAGMVPFKDVFLGAEKRPYTRAASAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEQAIAFAWKFLTEELQLPKEKLWVTVFTEDDEAADIWINQIGVPKERVSRIGAKDNFWSMGDTGPCGPCSEIFYDHGEEVWGGPPGTPEEDGDRYIEIWNLVFMQFNRHADGTMEPLPNPSIDTGMGLERIAAILQGVHSNYEIDLFQALIKAAAEVTNAQDMEDKSLRVIADHIRSCAFLVLDGVMPSNEGRGYVLRRIIRRAVRHGNKLGAKGTFFHKLVAELVAQMGDAYPELAKKQAIIEKVLRIEEEQFGKTLERGLAILEEHLSSLEG----DVIPGDVVFKLYDTYGFPADLTADVARERFLTIDEKGFQECMDVQRKKAQQAGKFGADYNTQLRSDKSTEFKGYDAQQHSATVVELFAGGEKVSVLNDGQEGIVILNRTPFYAESGGQVGDTGVLATANGEFIVTDTQKL-GNAFAHHGKV-VGQIGVNDKVDARIDAERRDRIKKNHTATHLIHEALRQELGEHVNQKGSLVQPDRLRFDFSHFEGVTPEQLQRIEKQVNDEIRRNFALQTELMAIDAAKEKGAMALFGEKYDDEVRVVSIGDYSIELCGGTHVERTGDIGLFKIVSEAGIAAGVRRIEAVTGSDALNHVAAQEEKLAQIAALLKADSANVLDKVQGLLERSKGLEKQVTQLNDKLASAAGASLLDAAKDINGVKVLIANVAGTESKALRGMVDDLK 785          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1356562816|ref|WP_105264468.1| (alanine--tRNA ligase [Pseudoalteromonas sp. T1lg76])

HSP 1 Score: 961.444 bits (2484), Expect = 0.000e+0
Identity = 465/791 (58.79%), Postives = 585/791 (73.96%), Query Frame = 0

Query:   59 LITHKAKNSALDAEEYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQ 849
            L T + +   LD   YF +  H +V S+SLVP NDPTLLF NAGMV FKDVFLGA+KR Y RA ++QRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKE+AI FAW+ LT++  L +EKLWVTVF EDDEA  IW + IGVPK+RV RIGAKDNFW MGDTGPCGPC+EIFYDHGE+V GGPPG+P+EDGDRYIEIWNLVFMQ++R +DGT+ PLP P +DTGMGLERIAA+LQ VHSNYEIDLF  LI  AAKV +  D  +KSLRVIADHIRS +FL++DGV+PSNEGRGYVLRRIIRRA+RHG ++G K  F H +V  LV +MG+AYPELA+++G IE++L+ E E+F +TLE GLA+L+ H+ S++      +I GDV+FKLYDTYGFP DLT DVARER L++D  GF+  M+ QR KA+ A  FG +    +    +TEF GY   Q SA VV ++     V  ++ G++ I++LN+TPFY ESGGQV D G L  AN  F V++ QK  G  FAH+G +  G I + D V+A I+ + R+ I  NH+ATHL+H AL   LG+HV QKGSLV+ADRLRFDFSHFE +T +++  +E+ VN E+R N+ + T LM I+ A+  GA ALFGEKYDD VRVV++G  SIELCGGTHV RTG+IGLF + SE+G+A+GVRRIEA+TG  A++    Q+  L  +A+ LK+   N LD  +  +++ + LEKQ A L  KLASA+    L   + IN V VL   +A  ++K L G  + ++
Sbjct:    4 LTTAQLRQKFLD---YFAKHQHQVVPSASLVPGNDPTLLFNNAGMVPFKDVFLGAEKRPYTRAASAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEQAIAFAWKFLTEELQLPKEKLWVTVFTEDDEAADIWINQIGVPKDRVSRIGAKDNFWSMGDTGPCGPCSEIFYDHGEEVWGGPPGTPEEDGDRYIEIWNLVFMQFNRHADGTMEPLPNPSIDTGMGLERIAAILQGVHSNYEIDLFQALISSAAKVTNAQDMEDKSLRVIADHIRSCAFLVLDGVMPSNEGRGYVLRRIIRRAVRHGNKLGAKGTFFHKIVAELVAQMGDAYPELAQKQGIIEKVLRIEEEQFGKTLERGLAILEEHLSSLEG----DVIPGDVVFKLYDTYGFPADLTADVARERFLTIDEKGFQECMDVQRKKAQQAGKFGADYNNQLRSDKSTEFKGYDAQQHSATVVEMFAAGDKVSLLNDGEEGIVILNRTPFYAESGGQVGDTGVLATANGQFIVTDTQKL-GNAFAHHGKV-VGQIGVNDKVDARIDAERRDRIKKNHTATHLIHEALRQELGEHVNQKGSLVQADRLRFDFSHFEGVTPEQLQRVEKKVNDEIRHNFALQTELMAIDAAKEKGAMALFGEKYDDEVRVVSIGDYSIELCGGTHVERTGDIGLFKIVSEAGIAAGVRRIEAVTGADALNHIAAQEEKLTQIAALLKADSGNVLDKVQGLLERSKGLEKQVAQLNDKLASAAGASLLDGAKDINGVKVLIANVAGTESKALRGMVDDLK 785          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1057286216|ref|WP_068649803.1| (alanine--tRNA ligase [Thiomicrospira sp. XS5] >gi|972925321|gb|KUJ75817.1| alanine--tRNA ligase [Thiomicrospira sp. XS5])

HSP 1 Score: 959.903 bits (2480), Expect = 0.000e+0
Identity = 482/808 (59.65%), Postives = 588/808 (72.77%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQAVAENVAKSHAQGHGMIVVLSAMSGETNRLL 880
            ++F +Q H  V SS +VP NDPTLLFTNAGMVQFK+ FLG + RDY+RAT+ QRC+RAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK EAIQ+AWQ LT++ GL E+KLWVTVFEED EAE IW   +GV  ER  R GAKDNFW MGDTGPCGPC+EIFYDHG DVAGGPPGSPDEDGDRYIEIWNLVFMQ+DRS DGTLTPLPKP VDTGMGLER+AAV+Q+VH+NY+IDLF  ++  AA++    D SN SLRVIADHIRS +F+I+DGVLPSNEGRGYVLRRIIRRAIRHGY++G K  F + LV TLV++MGEAYPEL K++ ++E+ L+ E ERF ETLE G+ +L+  I  +D      +I+G   FKLYDTYGFP+DLT DVARER LS+D  GFE +ME QRA+ARSAS+F    +  V +   TEF GY + +A+A+V+A++ G  +VER+  G + I++L+ TPFY ESGGQV D+G+L     SF V   QK  G  F H G +  G+IS+G  + A INT +R     NHSATHLLHAAL  VLG HV QKGSLV+ +RLRFDFSHFEPI+ +++L IEQ VN  +  N +V    M IE A+A GA ALFGEKY DVVRVV MG  SIELCGGTHV  TG IG F +TSESG+ASGVRRIEA+TG+ A    +  + +L  +A  LK+  A       + +   +ELEKQ   LQSK+AS+  DD    VE + EVNVL  ++   D   L  T ++++   E  A          +VL+A+ GE   L+
Sbjct:   12 DFFVKQGHTAVHSSPVVPGNDPTLLFTNAGMVQFKETFLGQEPRDYSRATSVQRCIRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWQFLTEELGLPEDKLWVTVFEEDQEAEDIWLKDMGVSAERFSRCGAKDNFWSMGDTGPCGPCSEIFYDHGPDVAGGPPGSPDEDGDRYIEIWNLVFMQFDRSEDGTLTPLPKPSVDTGMGLERLAAVMQKVHNNYDIDLFQAIVAKAAELTGEKDLSNSSLRVIADHIRSCAFMIVDGVLPSNEGRGYVLRRIIRRAIRHGYKLGQKDVFFYRLVPTLVEQMGEAYPELVKEQANVERALKLEEERFAETLENGMKILEEDIAQLDGT----VISGSTAFKLYDTYGFPLDLTADVARERQLSVDEAGFEKEMEAQRARARSASNFASQGQHKVDYNEPTEFAGYDQDEANAKVMAIFAGQESVERLDEGQEGIVILDTTPFYAESGGQVGDQGSLTEGMNSFHVDGCQKQGG-TFLHIGKVTAGAISVGQTIHAHINTKARRASERNHSATHLLHAALRQVLGTHVGQKGSLVQPERLRFDFSHFEPISAEQLLEIEQLVNHNIMLNADVQMEEMDIESAKAKGAMALFGEKYGDVVRVVDMGDFSIELCGGTHVNSTGEIGPFRITSESGIASGVRRIEAVTGEAAWQTIYDTEQVLLDIAGRLKTDKAQVDAKVTQLVNDQKELEKQLKQLQSKMASSQGDDLANAVEKVGEVNVLAAQLEGADVNTLRETLDKLKDKLEPAA----------IVLAAVDGEKVSLV 804          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1356548026|ref|WP_105254863.1| (alanine--tRNA ligase [Pseudoalteromonas sp. T1lg75])

HSP 1 Score: 959.133 bits (2478), Expect = 0.000e+0
Identity = 465/791 (58.79%), Postives = 584/791 (73.83%), Query Frame = 0

Query:   59 LITHKAKNSALDAEEYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQ 849
            L T + +   LD   YF +  H +V S+SLVP NDPTLLF NAGMV FKDVFLGA+KR Y RA ++QRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKE+AI FAW+ LT++  L++EKLWVTVF EDDEA  IW + IGVPKERV RIGAKDNFW MGDTGPCGPC+EIFYDHGE+V GGPPG+P+EDGDRYIEIWNLVFMQ++R +DGT+ PLP P +DTGMGLERIAA+LQ VHSNYEIDLF  LI  AA+V +  D  +KSLRVIADHIRS +FL++DGV+PSNEGRGYVLRRIIRRA+RHG ++G K  F H LV  LV +MG+AYPELAK++  IE++L+ E E+F +TLE GLA+L+ H+ +++      +I GDV+FKLYDTYGFP DLT DVARER L++D  GF+  M+ QR KA+ A  FG +    +    +TEF GY   Q SA VV ++     V  ++ G + I++LN+TPFY ESGGQV D G L  AN  F V++ QK  G  FAH+G +  G I + D V+A I+ + R+ I  NH+ATHL+H AL   LG+HV QKGSLV+ DRLRFDFSHFE +T +++  IE+ VN E+R N+ + T LM I+ A+  GA ALFGEKYDD VRVV++G  SIELCGGTHV RTG+IGLF + SE+G+A+GVRRIEA+TG  A++    Q+  L  +A+ LK+  AN LD  +  +++ + LEKQ + L  KLASA+    L   + IN V VL   +A  ++K L G  + ++
Sbjct:    4 LTTAQLRQKFLD---YFAKHQHQVVPSASLVPGNDPTLLFNNAGMVPFKDVFLGAEKRPYTRAASAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEQAIAFAWKFLTEELQLSKEKLWVTVFTEDDEAADIWINQIGVPKERVSRIGAKDNFWSMGDTGPCGPCSEIFYDHGEEVWGGPPGTPEEDGDRYIEIWNLVFMQFNRHADGTMEPLPNPSIDTGMGLERIAAILQGVHSNYEIDLFQALIKAAAEVTNAQDMEDKSLRVIADHIRSCAFLVLDGVMPSNEGRGYVLRRIIRRAVRHGNKLGAKGTFFHKLVAELVAQMGDAYPELAKKQAIIEKVLRIEEEQFGKTLERGLAILEEHLSTLEG----DVIPGDVVFKLYDTYGFPADLTADVARERFLTIDEKGFQECMDVQRKKAQQAGKFGADYNTQLRSDKSTEFKGYDAQQHSATVVEMFAEGDKVSVLNDGQEGIVILNRTPFYAESGGQVGDTGVLATANGEFIVTDTQKL-GNAFAHHGKV-VGQIGVNDKVDARIDAERRDRIKKNHTATHLIHEALRQELGEHVNQKGSLVQPDRLRFDFSHFEGVTPEQLQRIEKQVNDEIRRNFALQTELMAIDAAKKKGAMALFGEKYDDEVRVVSIGDYSIELCGGTHVERTGDIGLFKIVSEAGIAAGVRRIEAVTGADALNHIAAQEEKLAQIAALLKADSANVLDKVQGLLERSKGLEKQVSQLNDKLASAAGASLLDGAKDINGVKVLIANVAGTESKALRGMVDDLK 785          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|740300064|ref|WP_038137676.1| (alanine--tRNA ligase [Hydrogenovibrio sp. Milos-T1])

HSP 1 Score: 957.977 bits (2475), Expect = 0.000e+0
Identity = 470/808 (58.17%), Postives = 581/808 (71.91%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQAVAENVAKSHAQGHGMIVVLSAMSGETNRLL 880
            ++F +Q H  V SS +VP NDPTLLFTNAGMVQFK+ FLG + RDY RAT+ QRC+RAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK EAIQFAW  LTK+  L EEKLW+TVFEED EAE IW   + V   R  R GAKDNFW MGDTGPCGPC+EIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQ+DRS+DGTLTPLPKP VDTGMGLER+AAVLQ VH+NY+IDLF  ++  A+++    D +N SLRVIADHIRS +F+I+DGVLPSNEGRGYVLRRI+RRAIRHGY++G    F H LV+ LV++MG+AYPELAK++ ++E+ L+ E ERF ETLE G+ +L+  I  +DS    K+I+G   FKLYDTYGFP+DLT DVARERGLS+D  GFE +ME QR +ARSAS+F    K  +     T+F+GY + +    + A++ G+  V+    G DAI++L+QTPFYGESGGQV D G+L     SF V + QK  G  F H G +  G + +G  + A ++  +R     NHSATHLLHAAL +VLG HVQQKGSLVE +RLRFDF+HFEPI+D+++L IE+ VN  V  N  V T  M I+ A+  GA ALFGEKY DVVRVV MG  SIELCGGTHV  TG IG F + SESG+ASGVRRIEA+TG+ A +  ++ +  L ++AS +KS  A   +   + +   RE EK    LQSKLAS+   D    V  + +VNVL   +   D   L    E + ++ + +  +       ++VLSA+SG+   L+
Sbjct:   12 DFFVQQGHTAVHSSPVVPANDPTLLFTNAGMVQFKETFLGQEVRDYTRATSVQRCIRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQFAWDFLTKELKLPEEKLWITVFEEDQEAEDIWLKEMKVDASRFSRCGAKDNFWSMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQFDRSADGTLTPLPKPSVDTGMGLERLAAVLQNVHNNYDIDLFKAIVKKASELTGEKDLANSSLRVIADHIRSCAFMIVDGVLPSNEGRGYVLRRIVRRAIRHGYKLGQTQAFFHQLVDVLVEQMGDAYPELAKEQANVERALKLEEERFAETLENGMKILEEDIAGLDS----KLISGTTAFKLYDTYGFPLDLTADVARERGLSVDEAGFEKEMEAQRERARSASNFSSVSKGKIDFTGQTQFMGYHQDEGDGVIEAIFVGEQRVDTALEGQDAIVILDQTPFYGESGGQVGDAGSLTEGMHSFHVDDCQKQGG-AFLHIGRVTAGQLKVGQTLHAQVDVAARRASEKNHSATHLLHAALREVLGTHVQQKGSLVEPERLRFDFAHFEPISDEDLLKIEKLVNHNVMLNTPVCTEEMSIDAAKTKGAMALFGEKYGDVVRVVDMGEFSIELCGGTHVNSTGEIGPFRILSESGIASGVRRIEAVTGEGAWNAIYRNEQSLINIASAVKSDKAQVENKVLQLVADHREQEKLVKQLQSKLASSQGSDLASSVVKVGDVNVLAANIEGADGNTL---RETLDSLKDKLKPA-------VIVLSAVSGDKVTLV 804          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1249832244|gb|ATG89695.1| (alanyl-tRNA synthetase [Methylomonas koyamae])

HSP 1 Score: 957.592 bits (2474), Expect = 0.000e+0
Identity = 472/855 (55.20%), Postives = 592/855 (69.24%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQAVAENVAKSHAQGHGMIVVLSAMSGETNRLLDLAEKIDPNAVGRERDVLVSTGEQVTIALLAMALNKNGIAARSYTGG 927
            E+F ++ HA+  SSSLVP NDPTLLFTNAGMVQFKDVFLG +K D+NRA TSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI FAW  LTK+ G+   KLWVTVF+ED EAE IW + + +   R  RIGAKDNFW MGD GPCGPCTEIFYDHGE VAGGPPGSPDEDGDRYIEIWNLVFMQY+R  DG LTPLP P VDTGMGLERIAAV+Q VHSNYEID+F  L+  AA +   TD +N SLRVIADHIRS +FL+ DGVLPSNEGRGYVLRRI+RRAIRHGY++G +  F + LV  L  EMG AYPEL K +  +E++L++E ERF ETL  G+ +L++ +  +D      +I GDV+F LYDTYGFPVDLT D ARE  LS+D  GFE +M  QR +AR+   F  +   ++ H + TEF GY     S +V+ L+K    VE + AGD+ +++L+QTPFY ESGGQV D G +     SF+V++ QK  GQ+F H G + KG+I+ G   E S+N + R+    NHSATHLLHAAL  VLG+HVQQKGS V A+RLRFDFSHFEP+T ++I A+E+ VN+++R N  V    M  +EA   GA ALFGEKY D VRV+ MG  S ELCGGTHV RTG+IGL  + SE+GVA+GVRR+EA+TG+ A++   Q++  L  +A  LK++     D  ++ ++K R LEK+   L+ KLAS++ D+   Q   +N V VL VK+  VD K L    ++I+    + A          +VL+ +SG+    + L   +  +A+G+     V  GE V      +     G    +  GG
Sbjct:   16 EFFRQRGHAVRPSSSLVPGNDPTLLFTNAGMVQFKDVFLGREKVDFNRAATSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAINFAWDFLTKEMGIPPAKLWVTVFDEDSEAEAIWLEEVKIDPTRFSRIGAKDNFWAMGDVGPCGPCTEIFYDHGEHVAGGPPGSPDEDGDRYIEIWNLVFMQYERDKDGNLTPLPAPSVDTGMGLERIAAVMQGVHSNYEIDIFQNLVKTAAALAGTTDLTNSSLRVIADHIRSCAFLVADGVLPSNEGRGYVLRRIVRRAIRHGYRLGIQDTFFYKLVAPLAAEMGAAYPELVKAQEQVERVLKKEEERFAETLGQGMKILENCVAKLDG----HVIPGDVVFLLYDTYGFPVDLTADFAREHNLSVDHAGFEVEMSAQRDRARAGGQFAADYDQDIKHDSVTEFTGYSHLDGSVQVIGLFKKGQPVESLEAGDEGVVILDQTPFYAESGGQVGDSGLIECTGGSFEVTDTQKQGGQLFLHKGRVVKGTIAEGMVCEVSVNAEIRKATERNHSATHLLHAALRSVLGEHVQQKGSQVNAERLRFDFSHFEPMTTEQIAAVERLVNQQIRMNNAVCAQTMAKDEAVKAGAMALFGEKYGDEVRVLTMGDFSTELCGGTHVQRTGDIGLLKIVSETGVAAGVRRVEAMTGEGALEWIAQREQALLSIAGLLKAAPDKTADKVQQLLEKNRHLEKELEKLKGKLASSAGDELTSQAVDVNGVKVLAVKLDDVDPKALRDLADQIKNKLGSAA----------LVLATVSGDK---VSLIASVSKDAMGK-----VKAGELVNFVATQVGGKGGGRPDMAQAGG 848          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|495760552|ref|WP_008485131.1| (alanine--tRNA ligase [Gallaecimonas xiamenensis] >gi|407201682|gb|EKE71679.1| alanyl-tRNA ligase [Gallaecimonas xiamenensis 3-C-1])

HSP 1 Score: 957.592 bits (2474), Expect = 0.000e+0
Identity = 464/783 (59.26%), Postives = 578/783 (73.82%), Query Frame = 0

Query:   74 YFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQ 849
            YF ++ H IV SSSL+P NDPTLLFTNAGMVQFKDVFLG+DKR Y RAT+SQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKE AIQ+AW+ LT+   L  EKLWVTV+E DDEA  IW   +GVP +R+IRIG       A DNFW MGDTGPCGPCTEIFYDHGE + GGPPGSP+EDGDRYIEIWN+VFMQ++R  DG + PLPKP VDTGMGLERI+A+LQ VHSNYEID+F  LI  AAK +   D  NKSLRV+ADHIRS SFL++DGV+PSNEGRGYVLRRI+RRA+RHG Q+G   PF + LV  L+  MG+AYPEL +++  IE+ L  E E+F +TLE GL LLD  +  I  +    +I GDV+FKLYDTYGFPVDLTNDVARERGL+LD  GFET ME QR +A+ AS+FG +    +     T+F GY   +  + VVAL+KG  AV+ + AGD+ ++VL +TPFYGESGGQV D G L +  A   + N  K +G  F H+G + +GSI +GD ++A ++   R+ I  NHSATHL HAAL  VLG+HV QKGSLV+++R+RFDFSHFE +   EI  IE+ VN E+RAN+ VTT LM IEEA+A GA ALFGEKYDD VRVV MG  SIELCGGTHV+RTG+IGLF + SE G+A+GVRRIEA+TG+ A++    ++ LL  +A +LK+ VA   D   + + K ++LEK+    +++LA+++     +Q E +    +L  ++   D K L    ++++
Sbjct:   15 YFKDKGHQIVPSSSLIPGNDPTLLFTNAGMVQFKDVFLGSDKRAYTRATSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEGAIQYAWEFLTQVVKLPAEKLWVTVYETDDEAYDIWAKEVGVPTDRIIRIGDNKGAPYASDNFWSMGDTGPCGPCTEIFYDHGEHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRHGDGRMEPLPKPSVDTGMGLERISAILQHVHSNYEIDIFQHLIKAAAKAIGSEDLENKSLRVVADHIRSCSFLVVDGVMPSNEGRGYVLRRIVRRAVRHGAQLGASEPFFYKLVAALIDVMGDAYPELKEKQAIIEKALLMEEEQFRKTLERGLKLLDEALADIQGS----VIPGDVVFKLYDTYGFPVDLTNDVARERGLTLDEAGFETAMEAQRQRAKQASNFGADYNSRLDIDCQTQFSGYDGVRDVSPVVALFKGGDAVDSVQAGDEVLVVLERTPFYGESGGQVGDTGELSSDGARILIHNTTK-SGDAFVHHGKVAEGSIKLGDKLKAEVDKTRRDAIRLNHSATHLAHAALRKVLGEHVTQKGSLVDSERMRFDFSHFEGVKGVEISEIERLVNNEIRANHAVTTRLMQIEEAKAAGAMALFGEKYDDEVRVVQMGDFSIELCGGTHVSRTGDIGLFKVISEGGIAAGVRRIEAVTGEAALEWLQGREQLLGELAGSLKTDVAGLADRFAQQLDKTKQLEKELEKAKAQLAASAGSALAEQAEDLGGTKLLVAELPGADPKSLRELVDKLK 792          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1310923457|ref|WP_101051630.1| (alanine--tRNA ligase [Methylomonas sp. Kb3] >gi|1304539441|gb|PKD42209.1| alanine--tRNA ligase [Methylomonas sp. Kb3])

HSP 1 Score: 956.822 bits (2472), Expect = 0.000e+0
Identity = 464/808 (57.43%), Postives = 583/808 (72.15%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQAVAENVAKSHAQGHGMIVVLSAMSGETNRLL 880
            E+F +  HA+  SSSLVP NDPTLLFTNAGMVQFKDVFLG +K D+ RA TSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI FAW  LTK+ G+ +E+LWVTVF+ED EAE IW + +    +R  RIGAKDNFW MGD GPCGPCTEIFYDHGE VAGGPPGSPDEDGDRYIEIWNLVFMQYDR  DG LTPLP P VDTGMGLERI+AV+Q VHSNYEIDLF  L+  AA++   TD +N SLRVIADHIRS +FL+ DGVLPSNEGRGYVLRRI+RRAIRHGY++G +  F + LV  LV EMGEAYPELA  +  +E++L++E ERF ETLE G+ +L++ +  +D      +I GDV+F LYDTYGFPVDLT D ARE  LS+D  GFE +M  QR +AR+  SF  +   ++ H ++TEF GY     S +++ L+K    VE + AGD+ +++L+QTPFY ESGGQV D G +   +A F+V + QK  G++F H G + +G++S G A E S++ + R+    NHSATHLLHAAL   LG+HVQQKGS V ++RLRFDFSHFEP+T  E+  +E+ VN ++R N  V   +M  ++A   GA ALFGEKY D VRV+ MG  S ELCGGTHV RTG+IGLF +  E+GVA+GVRR+EA+TGQ A+D   Q++  L  +A  LK++   A D   + M+K R LEK+   L++KLAS++ D+   Q   +N V VL VK+  VD K L    ++++    + A          +VL+A+SG+   L+
Sbjct:   12 EFFRQHGHAVRPSSSLVPGNDPTLLFTNAGMVQFKDVFLGREKLDFTRAATSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHFAWDFLTKELGIPKERLWVTVFDEDSEAEAIWLEDVKHDPKRFSRIGAKDNFWSMGDVGPCGPCTEIFYDHGEHVAGGPPGSPDEDGDRYIEIWNLVFMQYDRDKDGNLTPLPAPSVDTGMGLERISAVMQGVHSNYEIDLFQNLLKVAAQLAGTTDLANSSLRVIADHIRSCAFLVADGVLPSNEGRGYVLRRIVRRAIRHGYRLGIQDTFFYKLVAPLVAEMGEAYPELANAQEQVERVLKKEEERFAETLEQGMKILEACVAKMDG----HVIPGDVVFLLYDTYGFPVDLTADFAREHKLSVDHAGFEVEMAAQRDRARAGGSFAADYDQDIKHDSSTEFTGYFHLDGSVQILGLFKQGQPVEALEAGDEGVVILDQTPFYAESGGQVGDTGRIECGDAVFEVHDTQKQGGKLFLHKGKVLRGTLSEGQACEVSVDAEIRKATERNHSATHLLHAALRATLGEHVQQKGSQVNSERLRFDFSHFEPMTPAEVATVERLVNEQIRLNNAVAAEIMAKDDAVKAGAMALFGEKYGDEVRVLTMGEFSTELCGGTHVQRTGDIGLFKIIGETGVAAGVRRLEAVTGQGALDWIDQREKALLSIAGLLKAAPDKAADKVHQLMEKNRGLEKELEKLKAKLASSAGDEMTSQAVDVNGVKVLAVKLDDVDPKALRDLADQLKNKLGSAA----------LVLAAVSGDKVSLI 805