BLAST of sodium-potassium-chloride cotransporter vs. L. salmonis genes
Match: EMLSAG00000003059 (supercontig:LSalAtl2s:LSalAtl2s1734:14347:19068:1 gene:EMLSAG00000003059 transcript:EMLSAT00000003059 description:"maker-LSalAtl2s1734-augustus-gene-0.5")

HSP 1 Score: 574.704 bits (1480), Expect = 0.000e+0
Identity = 316/755 (41.85%), Postives = 459/755 (60.79%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNN----CTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDE--DDDEDLDTGMTL-------KISSILGDKLKKSRQRHRSKR-----RSVFTGQNGVAVP------------QVILDDLNVFKQKKKL--GVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDI-------SSKAG---------------------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP VAIPKG                    TL  ++ TYV+Y  Y +MI +    EASGD  E + ++N+    EV +  N    C  R C YG  + QQ +  IS    L+YAGCFAATLSSAIASLVGAPRV QA+AKDKL+P I  F+ G GKNN+P +GY+L F I+L C+++G+L+ VSSLLSNFFVAAYALINFSVFHAS+ KSPGWRP F+YYN+W+SL GT LC+ VMFLMD +TA +T  +V  LY Y+  RKPE +WGSST+AQ FM  L +V  L+   DHVKNYRPKIMV SG+P +R  L+DFA+L+TK++SLL   D + ED+  K+  +IK     W  ++ +K F  V       +GA + M L+GLGK   NMIL+GF+  W   +   + YY I+  A D+ L++ +LR+  G D +       ++E + + ++ + L YD+  + +  +D+G+ L         S+  G+  K+     R+K+     + +F  +   ++P            + I++ L+ F+ K  +  G IDV+WLYDDG        IL+ R +++ C++RVF L +  D+LD +T++M SLL KFRID++DV+++ D T +  + T+ +F  +       +S +G                     ++   +I N+E++N  LR++E +K+ S+++ ++VMTLPMP+  ++   +YM+WL+  TKD+  PFL VRGNQ+SVLTFYS
Sbjct:  408 LSGDLKDPGVAIPKG--------------------TLLXIVSTYVTYTIYGVMIAAXTXREASGDVLELD-YVNETIK-EVNNITNSFWECENRTCSYGILHSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVFQAVAKDKLFPGIASFADGWGKNNDPVKGYLLVFLISLICILIGDLNYVSSLLSNFFVAAYALINFSVFHASITKSPGWRPGFKYYNKWISLLGTVLCICVMFLMDMVTALVTFIIVFFLYMYVSIRKPEVNWGSSTQAQSFMTALNSVLSLTKVEDHVKNYRPKIMVMSGSPLNRPTLIDFANLVTKRISLLESVDVVPEDLDWKKSQAIKNSGQNWFTENHIKGFFSVVRNSKISEGARTAMELSGLGKLRTNMILLGFQDTWWKKDESAKEYYKIIQTAFDLRLAVGVLRINGGLDVTSMTN---LME-DSSPNQSNALNYDDRCESNMSVDSGLDLATKSGAKNTSTQSGEDDKEDGSLKRNKKPNGFEKLLFKKKCPSSIPLPPSEKDVKPLDEDIVNRLSQFRDKTTVNTGTIDVYWLYDDGXXXXH---ILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVPPLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLIVMTLPMPKKEIVPQELYMAWLDYTTKDM-PPFLLVRGNQTSVLTFYS 1132          

BLAST of sodium-potassium-chloride cotransporter vs. L. salmonis genes
Match: EMLSAG00000009267 (supercontig:LSalAtl2s:LSalAtl2s58:680408:685072:1 gene:EMLSAG00000009267 transcript:EMLSAT00000009267 description:"augustus_masked-LSalAtl2s58-processed-gene-6.1")

HSP 1 Score: 408.683 bits (1049), Expect = 8.124e-131
Identity = 204/425 (48.00%), Postives = 289/425 (68.00%), Query Frame = 0

Query:  273 EAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIR-EDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDI--SSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            + QQF+ TL+++  L+ TP+HVKNYRPK++VF+G P+HR PL+DFA+L+TKKLSLLMCA +   D   K  + ++     W   HK+K+F+ +   KNFE+GA++CMNLAG+GK  PNM+L G+K DWL+D  G + Y ++LH A D++L + ILRL  GCDFS+ +  E   + E+  DEE++      D+++   G +L+        L++ + R R    +V+TG +G  +P+  + D+  F+ KK+ G IDVWWLYDDGGLTLLLP IL  R QFA CQLRVFAL +  D+LD ET+NM  LL KFRI Y  V +IPDVT +A + T+ +F  I    K  +    +I  +E++NR+LRL+E L+E+S  S+M++MTL +PR     + +YMSWLE+MTK++  PFL +RGNQSSVLTFYS
Sbjct:  537 KXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHVNIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLAGIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKVIGEEEQQQHEIELDEENE------DEKNGKQGKSLE--------LQQKKVRXRKVSTAVYTGIDGNPLPKNTVQDITQFQIKKRKGNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKILDEIKPPLEESEIISQKEKTNRHLRLTELLREHSKRSEMIIMTLSIPRRGTAVAQLYMSWLEMMTKNM-PPFLLIRGNQSSVLTFYS 946          

HSP 2 Score: 196.438 bits (498), Expect = 4.436e-53
Identity = 96/182 (52.75%), Postives = 122/182 (67.03%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRK----CEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMV 178
            +SGDLKDP VAIPKG                    TL A+I+T+++Y+GY  +I     +EASG+  EY  ++N         FNNC+ R     C+YG+SNDQQ + +ISYT YL+YAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFI +F+ G G NN+P RGY L F I++ C+++
Sbjct:  364 LSGDLKDPGVAIPKG--------------------TLLAIIVTFITYIGYGTIITGVMHTEASGNQTEYENWIN--GVEGALYFNNCSGRPADDFCQYGSSNDQQAMERISYTGYLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIHIFAPGFGANNDPIRGYFLVFIISMGCILI 523          

BLAST of sodium-potassium-chloride cotransporter vs. L. salmonis genes
Match: EMLSAG00000007778 (supercontig:LSalAtl2s:LSalAtl2s455:327857:377250:-1 gene:EMLSAG00000007778 transcript:EMLSAT00000007778 description:"maker-LSalAtl2s455-augustus-gene-2.20")

HSP 1 Score: 167.162 bits (422), Expect = 3.259e-43
Identity = 91/299 (30.43%), Postives = 143/299 (47.83%), Query Frame = 0

Query:  112 LVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSH----RVPLMDFAHLLTKKLSLLMCADIREDVTIK---EKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDE 403
            +V  G   +T+ + + SL GAPR+LQA+AKD + PF+  F     K  EP R   ++  I    V++GN+D ++ LLS FF+  Y  +N +    +++++P WRP F+YY+  +SL G  LC+AVMF+     A + + + GI+Y YI YR  E  WG             ++  L   P H KN+RP+I++ +   S        L   A  L     L +   + +    K   E  + K    + +    +K F  V   K+  DG        GLG   PN +++G+   W   E
Sbjct:  535 VVEIGSLLSTIGAGLQSLTGAPRLLQAIAKDGIIPFLAPFQVQSAKG-EPVRALGITLLICEVAVLIGNVDEIAPLLSMFFLMCYGFVNLAXALQTLLRTPNWRPRFKYYHWSLSLLGVVLCIAVMFMSSWYYALLAMGIAGIIYKYIEYRGAEKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILAKLDSQLKVKHKRLFALASQLKAGKGLTIGVTVLDGHFGKMYGEAQAAKFSIQQVIKDEAVKGFADVVVSKDISDGVCHLTQTTGLGGMKPNTVILGWPNSWRKKE 832          

HSP 2 Score: 62.7734 bits (151), Expect = 2.352e-10
Identity = 55/204 (26.96%), Postives = 97/204 (47.55%), Query Frame = 0

Query:  520 KKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSKAGITTDMLIENQERS--NRYLRLSESLKEYSTNSQMVVMT-------------------LP-----MPRPSMISSGI---YMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            K  G ID+WW+  DGGL +LLP +LK  + + NC+LR+F++    D+     K++ + L   RI+  DV ++    ++ SE T    L +  +  +  +M + ++ +    R L L++ + EY     +++ T                   +P     +P     S  I   +M +LE++T+ L+   L VRG    V+T YS
Sbjct:  868 KVKGNIDIWWIVHDGGLLMLLPFLLKQHRTWKNCKLRIFSVAQHEDNSIQMKKDLKTFLYHLRIE-ADVEVVEMQDSDISEYTYERTLLMEQRNEMLKEMRVSDKRKGKMTRRLSLNKGVGEYKVVDDIIIHTDKVTNEIEVETVIENSRADVPNIEDKIPPKVKFSEEIDEVHMEFLEVLTEGLER-VLMVRGGGREVITIYS 1069          

BLAST of sodium-potassium-chloride cotransporter vs. L. salmonis genes
Match: EMLSAG00000003697 (supercontig:LSalAtl2s:LSalAtl2s1:1949199:1954526:1 gene:EMLSAG00000003697 transcript:EMLSAT00000003697 description:"maker-LSalAtl2s1-augustus-gene-19.17")

HSP 1 Score: 122.479 bits (306), Expect = 5.051e-29
Identity = 97/409 (23.72%), Postives = 178/409 (43.52%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMD-TMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIRE-DVTIKEKDSIKADANEW--LVKH-KMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEM 404
            +SGDLK+P+ +IPKG                    TL A   T+++YV    +                 A   D   L          R C Y        + + S+    V  G   AT  ++ + L+G+ RVL+A++KD ++     + +     N P    ++++ ++     +G ++ ++  ++  F+ +YA +N S     +  +P +RP FR ++ +    G    + +MF+++  ++A +  A +            + +WGS ++A  F    + +  L    DHVK +RP++++   NP +   L+DFA+ L KK  L +   +RE +    E+D        W  LV H K+KAF  +        G    + ++G+G   PN I+ GF  +  D+ M
Sbjct:  267 LSGDLKEPSKSIPKG--------------------TLLASFFTFITYVILFTL----------------TAMTCDKELL---------LRDCSY--------MIEFSFWKPFVIIGTVLATFCASTSCLIGSSRVLEAISKDTMFGIFLEWITYGTVGNNPVLAVVVTWLLSNIFFFIGGVNTIAQFVAVLFLLSYASVNLSCLSLDLASAPNFRPIFRCFSWYTCALGLIGTLTMMFMINPVISALVLFACIFFFLSLSFLSPTKENWGSISQALLFHQVRKYLLLLDSRKDHVKFWRPQVLLLVSNPRNHCSLIDFANAL-KKGGLYVLGHVREGNFEGMEEDECGLLMPHWLNLVDHLKVKAFVELTVAPTVRSGIQQLIRISGIGAMKPNTIIFGFPYE--DENM 619          

BLAST of sodium-potassium-chloride cotransporter vs. L. salmonis genes
Match: EMLSAG00000004234 (supercontig:LSalAtl2s:LSalAtl2s222:1210791:1213007:1 gene:EMLSAG00000004234 transcript:EMLSAT00000004234 description:"maker-LSalAtl2s222-snap-gene-12.7")

HSP 1 Score: 119.013 bits (297), Expect = 3.197e-28
Identity = 80/293 (27.30%), Postives = 144/293 (49.15%), Query Frame = 0

Query:  113 VYAGCFAATLSSAIASLVGAPRVLQALAKDKLY-PFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAH--WGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIRED-VTIKEKDSIKADANEWLVK---HKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTD 398
            V  G F AT S+++ +L+G  R+L+A+AKD ++ P       G  K N P    I ++ +    +M+G+L+ ++ L S  F+ +Y  IN +     +  +P +RP ++Y++ +  L G    + +MF +  + + + I +   L   +    P  H  WGS ++A  F    + +  L     HVK +RP++++   NP     L++F + L KK  L +   +  D +   +KD    +   WL      K+KAF  V   ++   G    + ++G+G   PN +L+GFK D
Sbjct:  283 VTIGVFMATFSASLNNLIGXSRILEAIAKDVVFGPLFNFVLKGTYKEN-PVTAVISTWFVVELFLMMGSLNNIAQLCSVLFLLSYGTINLTCLFLHLASAPNFRPTYKYFSTYTCLIGLLGSIIMMFAVSAIYSAVGILLCLSLILLLNLFSPIRHTNWGSISQALIFHQVRKYLLLLDQRKSHVKFWRPQMLLLVSNPRRNCSLIEFGNAL-KKSGLYVLGHVHVDNLEEHDKDPTLNEIPHWLKLIDFLKVKAFVEVTIARSLRLGVDQLVRISGIGAMKPNTVLLGFKDD 573          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|1709292|sp|P55011.1|S12A2_HUMAN (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1)

HSP 1 Score: 531.561 bits (1368), Expect = 1.149e-173
Identity = 288/740 (38.92%), Postives = 432/740 (58.38%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEV-----------FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIR---EDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEM-GTERYYNILHQALDMNLSLTILRLPRGCDFSQ-HVQHECIVEVEVTNDEEDDLCYDE-DDDEDLDTGMTLK---ISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVP-----QVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF---------------LDISSKAG------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL DP  AIPKG                    TL A+++T + YVG A+ +GSC + +A+G+       +ND    E+           F F++C    C YG  N+ Q +S +S  + L+ AG F+ATLSSA+ASLV AP++ QAL KD +YP   +F+ G+GKNNEP RGYIL+F IAL  +++  L+V++ ++SNFF+A+YALINFSVFHAS+ KSPGWRPAF+YYN W+SL G  LC  VMF+++   A +T  +V  LY Y+ Y+KP+ +WGSST+A  ++N L++   LS   DHVKN+RP+ +V +G P+ R  L+   H  TK + L++C  +        +KE    +A    WL+K+KMKAF+   +  +  +GA   M  AGLG+  PN +++GFK DWL  +M   + Y N+ H A D+   + ++RL  G D S    Q E +   E +   +D +   E     DLDT   L    I+  + ++  K+  +   K+ S      G  VP     Q +L+    F++K+    IDVWWL+DDGGLTLL+P +L  +K++ +C++RVF +G  ++ +D + + M +LL KFRID+ D++++ D+ T+  +     F                DI+ K        IT + L   + ++ R +RL+E LKE+S+ + ++VM+LP+ R   +SS +YM+WLE ++KDL  P L VRGN  SVLTFYS
Sbjct:  507 ISGDLADPQSAIPKG--------------------TLLAILITTLVYVGIAVSVGSCVVRDATGN-------VNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKES-----KGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDL-PPILLVRGNHQSVLTFYS 1212          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|341941994|sp|P55012.2|S12A2_MOUSE (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1)

HSP 1 Score: 530.02 bits (1364), Expect = 4.183e-173
Identity = 285/740 (38.51%), Postives = 433/740 (58.51%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEV-----------FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIR---EDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEM-GTERYYNILHQALDMNLSLTILRLPRGCDFSQ-HVQHECIVEVEVTNDEED---DLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQN-GVAVP-----QVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF---------------LDISSKAG------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL DP  AIPKG                    TL A+++T V Y+G A+ +GSC + +A+G+       +ND  T E+           F F+ C    C YG  N+ Q +S +S  + L+ AG F+ATLSSA+ASLV AP++ QAL KD +YP   +F+ G+GKNNEP RGYIL+F IAL  +++  L+V++ ++SNFF+A+YALINFSVFHAS+ KSPGWRPAF+YYN W+SL G  LC  VMF+++   A +T  +V  LY Y+ Y+KP+ +WGSST+A  +++ L++   LS   DHVKN+RP+ +V +G+P+ R  L+   H  TK + L++C  +        +KE    +A    WL+K+KMKAF+   +  +  +GA   M  AGLG+  PN +++GFK DWL  +M   + Y N+ H A D+   + ++RL  G D S    Q E +   E +   +D   ++ Y +  D+D     T K S       K   +  ++  + +   ++ G  VP     Q +L+    F++K+    IDVWWL+DDGGLTLL+P +L  +K++ +C++RVF +G  ++ +D + + M +LL KFRID+ D++++ D+ T+  +     F                DI+ K        IT + L   + ++ R +RL+E LKE+S+ + ++VM+LP+ R   +SS +YM+WLE ++KDL  P L VRGN  SVLTFYS
Sbjct:  500 ISGDLADPQSAIPKG--------------------TLLAILITTVVYIGIAVSVGSCVVRDATGN-------VNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQD-----TCKSSGEKSITQKDEEEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDL-PPVLLVRGNHQSVLTFYS 1205          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|1709294|sp|P55013.1|S12A2_SQUAC (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; AltName: Full=NKCC; AltName: Full=Na-K-CL symporter)

HSP 1 Score: 519.235 bits (1336), Expect = 3.632e-169
Identity = 281/735 (38.23%), Postives = 425/735 (57.82%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASG---DAAEYNAFLN-DNATLEV-FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIR---EDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMG-TERYYNILHQALDMNLSLTILRLPRGCDFSQ-HVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKIS-----SILGDKLKKSRQRHRSKRRSVFTGQNGVAVP-----QVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDI---------------SSKAG------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL DP +AIPKG                    TL A+++T + Y G A+ +GSC + EA+G   DA       N  N   ++ F+F++C   KC YG  ND Q +S +S    L+ AG F+ATLSSA+ASLV AP++ QAL KD +YP + VFS G+GKNNEP RGY+L+F I L  +++  L+V++ ++SNFF+A+YALINFSVFHAS+ KSPGWRPAFR+YN W+SL G  LC  VMF+++   A +T  +V  LY Y+ Y+KP+ +WGSST+A  ++N L++   L+   DHVKN+RP+ ++ +G P+ R  L+   H  TK + L++C  +        +KE  + +A    WL+K+KMKAF+   Y ++  +G    +   GLG+  PN ++ GFK DW    M   E Y N +H A D    + ++RL  G + S    Q E     E +   +D +   E  D D     +  +S     ++  D+  +      S +  +       +VP     Q +L   + F++K+  G IDVWWL+DDGGLTLL+P +L  +K++ +C++RVF +G  ++ +D + + M +LL KFRID+ D+ ++ D+ T+ S+   T F ++               S K        IT + L   + ++ R +RL+E L+E S  + ++VM+LP+ R   +SS +YM+W+E ++KDL  P L VRGN  SVLTFYS
Sbjct:  479 ISGDLADPQLAIPKG--------------------TLLAILITTIVYAGAAVSVGSCIVREATGNLTDAIIPGTVTNCTNVACKLGFNFSSCATNKCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHVFSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFRFYNMWISLIGAILCCGVMFVINWWAALLTNVIVLALYIYVTYKKPDVNWGSSTQALTYLNALQHAIRLTGVEDHVKNFRPQCLLMTGAPTSRPALLHLVHAFTKNVGLVVCGHVHTGPRRQALKEISTDQAKYQRWLIKNKMKAFYAPVYAEDLREGTQFLLQAVGLGRMRPNTLVFGFKKDWRQALMKDVENYINAIHDAFDYQYGVVVIRLKEGFNISHLQAQEELCTSQEKSAHPKDIVVNLEHSDADSSKPSSKSVSETNSPAVCQDQKDEEDDGKASTQPLLKKEVKDPSVPLNMTDQKLLQASSQFQKKQGKGTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMIEPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLIVMSLPVARKGAVSSALYMAWIETLSKDL-PPILLVRGNHQSVLTFYS 1191          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|1709297|sp|P55016.1|S12A1_RAT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 503.442 bits (1295), Expect = 5.139e-164
Identity = 276/730 (37.81%), Postives = 423/730 (57.95%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASG---DAAEYNAFLNDNATLEV-FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI---REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGT-ERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYD---EDDDEDLDTGMTLKISSILGD----KLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEA---------------------TQTDFLDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL+DP  AIP+G                    T+ A+ +T V+Y+G AI + +C + +A+G   D        N +A   + + F+ C    C+YG  N+ Q +S +S    L+ AG F+ATLSSA+ASLV AP+V QAL KD ++  +  F+ G+GKNNEP RGY L+F IA+A +++  L+V++ ++SNFF+A+YALINFS FHAS  KSPGWRPA+  YN WVSL G  LC AVMF+++   A IT  +   LY Y+ Y+KP+ +WGSST+A  +++ L+N  +L+   DHVKN+RP+ +V +G P  R  L+D  H  TK   L +C ++      + +KE +S  A    WL+K+K+KAF+       F DG  S +  +GLG+  PN +++G+K +W    +   E Y  I+H A D  + + I+R+ +G D S  +Q    V+ E+   E++ L  +   +D+D +   G    +    G     K    +  + S  +S+  G+      Q +++    FK+K+  G IDVWWL+DDGGLTLL+P IL +RK++ +C+LR++ +G  ++ ++ E  +M SLL KFRI + D+ II D+  + ++                      T  + L   S   IT   L   +E+S R +RL+E L+E+S  + ++V++LP+ R   IS  +YM+WLEI+TK+L  P L VRGN  +VLTFYS
Sbjct:  396 ISGDLEDPQDAIPRG--------------------TMLAIFITTVAYIGVAICVRACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQ----VQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKPAPKKDSNISTIQSMHVGE----FNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNL-PPVLLVRGNHKNVLTFYS 1095          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|27151793|sp|P55014.2|S12A1_MOUSE (RecName: Full=Solute carrier family 12 member 1; AltName: Full=BSC1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 502.671 bits (1293), Expect = 8.569e-164
Identity = 274/730 (37.53%), Postives = 421/730 (57.67%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASG---DAAEYNAFLNDNATLEV-FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI---REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGT-ERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDE-------DDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEA---------------------TQTDFLDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL+DP  AIP+G                    T+ A+ +T V+Y+G AI + +C + +A+G   D        N +A   + + F+ C    C+YG  N+ Q +S +S    L+ AG F+ATLSSA+ASLV AP+V QAL KD ++  +  F+ G+GKNNEP RGY L+F IA+A +++  L+V++ ++SNFF+A+YALINFS FHAS  KSPGWRPA+  YN WVSL G  LC AVMF+++   A IT  +   LY Y+ Y+KP+ +WGSST+A  +++ L+N  +L+   DHVKN+RP+ +V +G P  R  L+D  H  TK   L +C ++      + +KE +S  A    WL+K+K+KAF+       F DG  S +  +GLG+  PN +++G+K +W    +   E Y  I+H A D  + + I+R+ +G D S  +Q    V+ E+   E++ L  +        ++ +    G+  K   +   K    +  + S  +S+  G+      Q +++    FK+K+  G IDVWWL+DDGGLTLL+P IL +RK++ +C+LR++ +G  +  ++ E  +M SLL KFRI + D+ II D+  + ++                      T  + L   S   IT   L   +E+S R +RL+E L+E+S  + ++V++LP+ R   IS  +YM+WLEI+TK+L  P L VRGN  +VLTFYS
Sbjct:  396 ISGDLEDPQDAIPRG--------------------TMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQ----VQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGE----FNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNL-PPVLLVRGNHKNVLTFYS 1095          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|212276464|sp|Q13621.2|S12A1_HUMAN (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 499.204 bits (1284), Expect = 2.049e-162
Identity = 274/731 (37.48%), Postives = 422/731 (57.73%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAE-YNAFLNDNATLEV---FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI---REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMG-TERYYNILHQALDMNLSLTILRLPRGCDFSQHVQ--------HECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEA---------------------TQTDFLDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL+DP  AIP+G                    T+ A+ +T V+Y+G AI +G+C + +A+G+  +   + +N N +      + F+ C    C+YG  N+ Q +S +S    L+ AG F+ATLSSA+ASLV AP+V QAL KD +Y  +  F+ G+GKNNEP RGYIL+F IA+A +++  L+ ++ ++SNFF+A+YALINFS FHAS  KSPGWRPA+  YN WVSL G  LC AVMF+++   A IT  +   LY Y+  +KP+ +WGSST+A  +++ L+N  +L+   DHVKN+RP+ +V +G P  R  L+D  H  TK   L +C ++      + +KE +S  A    WL+K+K+KAF+       F DG  S +  +GLG+  PN +++G+K +W    +   E Y  I+H A D  + + I+R+ +G D SQ +Q         +  + +E T   +D+ C +E        G+  K   +   K    +    +  +S+  G+      Q +++    FK+K++ G IDVWWL+DDGGLTLL+P IL +RK++ +C+LR++ +G  ++ ++ E   M SLL KFRI + D+ II D+    ++                      T  + L   +   IT   L   +E+S R +RL+E L+E+S  + ++V++LP+ R   IS  +YM+WLEI+TK+L  P L VRGN  +VLTFYS
Sbjct:  400 ISGDLEDPQDAIPRG--------------------TMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEAT--IKDNECEEESGG---IRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGE----FNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNL-PPVLLVRGNHKNVLTFYS 1099          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|1709296|sp|P55015.1|S12A1_RABIT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 497.664 bits (1280), Expect = 9.586e-162
Identity = 269/723 (37.21%), Postives = 416/723 (57.54%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASG---DAAEYNAFLNDNATLEV-FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI---REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMG-TERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEA---------------------TQTDFLDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL+DP  AIP+G                    T+ A+ +T V+Y+G AI +G+C + +A+G   D        N +A   + + F+ C    C+YG  N+ Q +S +S    L+ AG F+ATLSSA+ASLV A +V QAL KD +Y  +  F+ G+GKNNEP RGYIL+F IA+A +++  L+ ++ ++SNFF+A+YALINFS FHAS  KSPGWRPA+  YN WVSL G  LC AVMF+++   A IT  +   LY Y+ Y+KP+ +WGSST+A  +++ L+N  +L+   DHVKN+RP+  V +G P  R  L+D  +  TK   L +C ++      + +KE +S  A    WL+K+K+KAF+       F DG  S +  +GLG+  PN +++G+K  W    +   E Y  I+H A D  + + I+R+ +G D SQ +Q +  +E         +    +++ E+ + G+      +    + K   +  S   ++ +   G    Q +++    FK+K+  G IDVWWL+DDGGLTLL+P IL +RK++ +C+LR++ +G  ++ ++ E   M SLL KFRI + D+ +I D+  + ++                      T  + L   +   IT   L   +E+S R +RL+E L+E+S  + ++V++LP+ R   IS  +YM+WLEI+TK+L  P L VRGN  +VLTFYS
Sbjct:  400 ISGDLEDPQDAIPRG--------------------TMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGINCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAAKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCFVLTGGPMTRPALLDITYAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKKWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELEKLEQERLALEATIKDNECEEGNGGIRGLFKKVGKLNITKPTPKKDSSINTIQSMHVG-EFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINIKPNKESWKFFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLP-PVLLVRGNHKNVLTFYS 1099          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|2500792|sp|Q25479.1|NKCL_MANSE (RecName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter; AltName: Full=Na-K-CL symporter)

HSP 1 Score: 490.345 bits (1261), Expect = 2.109e-159
Identity = 271/736 (36.82%), Postives = 432/736 (58.70%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEY---NAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI-REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMG-TERYYNILHQALDMNLSLTILRLPRGCDF----SQHVQHECIVEVEVTND------EEDDLCYDEDDDEDL-DTGMTLKISSILGDKLKKSRQRHRS-----------------KRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSK---AGITTDMLIENQE------RSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDPA AIPKG                    TL A++++ VSY    +  G   L +ASG+  +    N  + D +++ + + NN     CEYG  N    +  +S     +Y GC+AATLS+A+ +L+  PR++QAL  D++YP +  FS  +G++ EP+RGY+L+F ++L  +++ +L+ ++ L+SNF++A+YALINF  FH ++V+  GWRP FRYYN W+SLAG  +CVA+M L+  + + +T A+   LY  + YR+P+ +WGSST+AQ +   L + + L+ T +HVKNY P+++V +G P  R  L+D  +L++K  SL++  DI +E ++ K + +     +EWL   K++AF    +  +FE GA + +  +G+G+ APN++LMG+K+DW          Y+N+LH A +  L++ I+R+  G D+    S+  +      +  T+         D L    D D D+ DT     +S++L   L  SR    S                  R+ V+   +G+ + +  L  +++F++K++ G +DVWWLYDDGGLT+LLP I+  R  +ANC+LR+FAL + + +++ E +NM +LL KFRIDY  + ++ D+T      T+  F +   K      + D  I + E      ++NR LRL E L   S ++++VVM+LPMPR   IS+ +YM+WLE+M++DL  P LFVRGN +SVLTFYS
Sbjct:  352 ISGDLKDPASAIPKG--------------------TLLALLISMVSYTLMVLFAGGGALRDASGNITDLLIVNGTVTDYSSVSLCALNNT----CEYGLHNSYSVMQLMSAWGPFIYGGCWAATLSTALTNLLSVPRLIQALGVDRIYPGLIFFSKPYGRHGEPYRGYVLTFFVSLLFLLIADLNTIAPLISNFYLASYALINFCTFHRALVRPLGWRPTFRYYNMWLSLAGFLMCVAIMLLVHWVMSLVTFAIFFTLYLIVHYRRPDVNWGSSTQAQMYKTALSSAHALARTGEHVKNYWPQLLVLAGRPQARPALVDLGNLISKAGSLMIVGDISQEKLSYKVRSARARSDDEWLRGRKVRAFCSRVHGFSFEPGARALVQGSGVGRLAPNVLLMGYKSDWTTCPANDLVSYFNVLHTAFENRLAVAIVRVSGGLDYSAVVSEGAEEGAAGSLTATSSSGELRVRRDGLIMHADSDLDIRDTQPKHNLSNLL--TLTTSRSFTISECKEKDKKKKERKPNDMHRQIVYNTASGLELSKFQLAQMSLFQKKQESGTLDVWWLYDDGGLTILLPYIISQRSAWANCKLRIFALANRLHEMELEERNMANLLAKFRIDYSSLTMVQDITDPPQPETKALFDETIKKFTEESASPDCRISDMELQTLAVKTNRQLRLRELLLANSKDARLVVMSLPMPRKGSISAPLYMAWLEMMSRDLP-PMLFVRGNHTSVLTFYS 1060          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|313104194|sp|P55017.3|S12A3_HUMAN (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl cotransporter; Short=NCC; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter)

HSP 1 Score: 442.58 bits (1137), Expect = 1.553e-141
Identity = 256/730 (35.07%), Postives = 393/730 (53.84%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEV-----------FSFNNCTFR-KCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIREDVTIKEKDSIKADAN---EWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGT-ERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISS---------------------KAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFY 693
            +SGDLKDPA+AIPKG                    TL A+  T +SY+  +  IGSC + +ASG        LND  T              ++F  CT +  C YG  N  QT+S +S  + L+ AG F ATLSSA+A LV A +V Q L +D+LYP IG F  G+GKN EP RGY+L++ IA+A +++  L+ ++ ++SNFF+ +YALINFS FHAS+  SPGWRP+F+YYN+W +L G  + V +MFL+    A I I VV  L  Y+ Y+KPE +WGSS +A  +   L     L+   DH+KNYRP+ +V +G P+ R  L+DF    T+ LSL++C  +      +    ++  AN   +WL K K+KAF+     ++   G    M  AGLG+  PN++++GFK +W      T E Y  ILH A D N  + ++R+  G + S+ +Q      +    D  +D    ++    +D    +K                  +  ++F  + G                KK    ID++WL+DDGGLTLL+P +L  +++++ C++RVF +G  ++ +D E K + SLL KFR+ + +V I+PD+           F D+ +                        I+ + + +N+ +S R +RL+E + +YS ++ ++V+TLP+ R     S +YM+WLE +++DL  P + +RGNQ +VLTFY
Sbjct:  360 ISGDLKDPAIAIPKG--------------------TLMAIFWTTISYLAISATIGSCVVRDASG-------VLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQ----AHINPVFDPAED---GKEASARVDPKALVK----------------EEQATTIFQSEQG----------------KK---TIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVF-VGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFY 1019          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|1717802|sp|P55019.1|S12A3_PSEAM (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter)

HSP 1 Score: 430.639 bits (1106), Expect = 7.587e-137
Identity = 252/727 (34.66%), Postives = 385/727 (52.96%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFR----------KCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDW-LDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSKAGITTD------------------MLIE-----NQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFY 693
            +SGDLKDP VAIP+G                    TL A+  T +SY+  +  IG+C + +ASG   E N  L+ +++ E  S   C +R           C++G  N  Q++S +S  + L+ AG F ATLSSA+A LV AP+V Q L KD+LYP IG F  G+GKN EP R Y+L++ IA+  V++  L+ ++ ++SNFF+ +YALINFS FHAS+  SPGWRP+FR+Y++W+SL G   CV +MFL+    A I   VV  L  Y  Y+KP  +WGSS +A  +   L     L+   DHVKNYRP+ +V +G P  R  L+D    LTK+LSL+MC      V       +      WL + K+++F+      +   G    +  AGLG+  PN++LMGFK DW  D       Y  ILH A D++  + +LR+  G D S   Q       +   +  + +C        + + +++                                 PQ      +VF++K+    ID++WL DDGGLTLLLP +L  RK++A C++RVF  G T D  + + + + +L++KFR+ + DV ++PD+           F D  ++  + T+                  M+ E     N+ +S R +RL+E L+ +S  + ++V+T+P+ R  +  S ++++WL+++++DL  P L VRGNQ +VLTFY
Sbjct:  357 ISGDLKDPTVAIPRG--------------------TLMAIFWTTISYLIISATIGACVVRDASG---ELNDTLSYSSSSENCSGLACQYRWDFSECIKNNTCKHGIMNYYQSMSLVSAFAPLISAGIFGATLSSALACLVSAPKVFQCLCKDQLYPLIGFFGKGYGKNAEPLRAYLLTYVIAVCFVLIAELNTIAPIISNFFLCSYALINFSCFHASVTNSPGWRPSFRFYSKWLSLLGAVCCVVIMFLLTWWAALIAFGVVFFLLGYTLYKKPAVNWGSSVQASSYSMALNQCVGLNQVEDHVKNYRPQCLVLTGPPCCRPALVDLVGCLTKRLSLMMCG----HVVTAGPSPVSERHVTWLNQRKVRSFYRGVVAADLRSGVNMLLQGAGLGRIKPNVLLMGFKKDWGCDSPQAAHHYIGILHDAFDLHYGVCVLRVKEGLDASHPPQCHVNPGFDGGPESINTVCAPACVQTSVTSSVSMDPD------------------------------PQ----PSSVFQKKQGKKTIDIYWLSDDGGLTLLLPYLLTRRKRWAGCKVRVFVGGDT-DKKEEQKEEVLALIKKFRLGFHDVEVLPDIHQPPQPGNVDHFEDSVNRFRLETNPKQDSDSGPQQQQQEEPWMITEQDLERNRAKSLRQIRLNEVLQVHSREAALIVITMPVGRRGVCPSTLFLAWLDVLSRDLRPPVLLVRGNQENVLTFY 1021          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1325281518|ref|XP_023325442.1| (solute carrier family 12 member 2-like [Eurytemora affinis])

HSP 1 Score: 716.457 bits (1848), Expect = 0.000e+0
Identity = 361/715 (50.49%), Postives = 483/715 (67.55%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLE----VFSFNNCTF--------RKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKS------RQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF---LDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDPA AIPKG                    TL A+  TY++Y+ Y +++   Y    SG A EY+ + ND+  LE    V  +++C+          +C +GT NDQQT++ IS+T YL+Y GCFAATLSSAIASLVGAPRVLQALAKDKLYP I  F+ G G NN+P RGYIL F I+L CV++G L++VSSLLSNFFVAAYALINFSVFHAS+ KSPGWRPAF+YY+ WVSL GT LCV VMF+MD +TA +T  +V +LYF+I YRKPEA+WGSS +AQ F++ L+ V  L+ T DHVKNYRPK++V +GNP+HR PL+DFA+L+TKKLSLL+CA +  +   K    ++     WL  HK+K F+ V    ++E+GA +C+ LAGLGK APN++L+GFK+DW+ +  G  +Y+N+LH A D  LS+ ILR+  G DFS+H+  E  V               E + ++ +     +    +G+   K+      RQR R+  R  +   +G  + + I+D +  F+ +KK G IDVWWLYDDGGLTLL+P IL  RK + +C LRVF LG+  DDLD +T+NM +LL KFRID+ DV++IPDVT  A +AT+ +    LD   +  I+ D L+ N+E++NR+LRL+E L+E+S  SQ +VMTLPMPR   +   +YM+WLEIMT+DL  P L +RGNQ+SVLTFYS
Sbjct:  275 LSGDLKDPATAIPKG--------------------TLLAIGTTYLTYILYGVVVAFTYSPYGSGVAEEYSIWTNDSIPLEEKMLVPRWDDCSADATLLRNGTECRFGTLNDQQTMAVISFTGYLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPKIEFFAPGFGANNDPIRGYILVFLISLGCVLIGELNMVSSLLSNFFVAAYALINFSVFHASITKSPGWRPAFKYYSPWVSLLGTLLCVCVMFMMDWVTALLTYVIVIVLYFFIYYRKPEANWGSSAQAQVFVSALKGVQSLTETQDHVKNYRPKVLVLTGNPAHRPPLVDFANLITKKLSLLICAHVITEPGSKHLALMRESTQAWLNDHKIKGFYTVTESGSWEEGAKACLTLAGLGKLAPNLVLIGFKSDWMTNLEGLHQYFNVLHSAYDHALSIGILRVKNGLDFSEHILAEETVGDHSEERRGSGDEEKEKEKKEDEEEGDGEDKDEIGELQSKTEVVQAVRQRTRTISRVTYETHDGTPLDKTIIDSIQQFQGEKKKGFIDVWWLYDDGGLTLLIPYILSTRKMYTDCSLRVFTLGNKKDDLDRDTRNMCTLLAKFRIDFSDVIVIPDVTKRAQDATKEELKQILDKLPEDCISEDDLLMNKEKTNRHLRLAELLREHSKESQQIVMTLPMPRKGSVCPELYMAWLEIMTRDL-PPTLLIRGNQTSVLTFYS 968          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1228359575|ref|XP_021952830.1| (solute carrier family 12 member 3-like [Folsomia candida])

HSP 1 Score: 677.167 bits (1746), Expect = 0.000e+0
Identity = 339/729 (46.50%), Postives = 477/729 (65.43%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATL---EVF-SFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIV----------EVEV-TNDEEDDLCYDEDDDEDLDTGMTLKIS------SILGDKLKKSRQRHRSKRRSV---FTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSK---------AGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDPA AIPKG                    TL A+ +TY+SY+GYA+M+G C + EASG+  EYN  +    T+   E F +++NCT R CEYG  N  Q +  IS    L+YAGCFAATLSSAIASLVGAPRV QAL KDKLYP I  F+ G+G NN+P RGY L F I+L C+++  L+ ++ LLSNFF+ AY LINFSVFHAS+  SPGWRPAF+YYN WVSL G  LC+AVMFL++  TA  T+A V  LY Y+ YRKPE +WGSST+AQ F + L+++ DL+   +HVKNYRP+I+V +G P +R PL+DFA+L+ K LSL++C   ++   + K K ++   A  WL++HK K F+ +  +++FE GA S M + GLGK  PNM+LMG+K DW+  +    + Y+N++H+ALDM+L++ ILR P G D+S  ++ E  +          E E+  N  E  L  D    E      + K+        ++ ++L       +S++ S+   + G  G  +P+ +L ++  F++K+K G IDVWWLYDDGGL++LLP +L  RKQ+A C LR+F+L S  D+L  E +NM +LL KFRIDY DV++IPD+T +A + T+ +F  + ++           IT   L+ ++E++NR+LRL E L+ +S +S  VVMTLPMPR   +S+ +YM+WLE++T+D+  PFL +RGNQ+SVLTFYS
Sbjct:  362 LSGDLKDPASAIPKG--------------------TLLAITITYLSYIGYAVMMGGCAMREASGNLTEYNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVIEEEGQLTPQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPDQVVNNQLVGFSSNKKSRKHSLSAMYKGPGGAILPRHVLSNITQFQRKQKKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVIAEFKSDDNQDGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFVVMTLPMPRKGTVSAPLYMAWLEMLTRDM-PPFLLLRGNQTSVLTFYS 1069          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1215275570|gb|OXA61879.1| (Bumetanide-sensitive sodium-(potassium)-chloride cotransporter [Folsomia candida])

HSP 1 Score: 677.167 bits (1746), Expect = 0.000e+0
Identity = 339/729 (46.50%), Postives = 477/729 (65.43%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATL---EVF-SFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIV----------EVEV-TNDEEDDLCYDEDDDEDLDTGMTLKIS------SILGDKLKKSRQRHRSKRRSV---FTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSK---------AGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDPA AIPKG                    TL A+ +TY+SY+GYA+M+G C + EASG+  EYN  +    T+   E F +++NCT R CEYG  N  Q +  IS    L+YAGCFAATLSSAIASLVGAPRV QAL KDKLYP I  F+ G+G NN+P RGY L F I+L C+++  L+ ++ LLSNFF+ AY LINFSVFHAS+  SPGWRPAF+YYN WVSL G  LC+AVMFL++  TA  T+A V  LY Y+ YRKPE +WGSST+AQ F + L+++ DL+   +HVKNYRP+I+V +G P +R PL+DFA+L+ K LSL++C   ++   + K K ++   A  WL++HK K F+ +  +++FE GA S M + GLGK  PNM+LMG+K DW+  +    + Y+N++H+ALDM+L++ ILR P G D+S  ++ E  +          E E+  N  E  L  D    E      + K+        ++ ++L       +S++ S+   + G  G  +P+ +L ++  F++K+K G IDVWWLYDDGGL++LLP +L  RKQ+A C LR+F+L S  D+L  E +NM +LL KFRIDY DV++IPD+T +A + T+ +F  + ++           IT   L+ ++E++NR+LRL E L+ +S +S  VVMTLPMPR   +S+ +YM+WLE++T+D+  PFL +RGNQ+SVLTFYS
Sbjct:  371 LSGDLKDPASAIPKG--------------------TLLAITITYLSYIGYAVMMGGCAMREASGNLTEYNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVIEEEGQLTPQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPDQVVNNQLVGFSSNKKSRKHSLSAMYKGPGGAILPRHVLSNITQFQRKQKKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVIAEFKSDDNQDGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFVVMTLPMPRKGTVSAPLYMAWLEMLTRDM-PPFLLLRGNQTSVLTFYS 1078          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1330891876|gb|PNF22995.1| (hypothetical protein B7P43_G10392 [Cryptotermes secundus])

HSP 1 Score: 663.3 bits (1710), Expect = 0.000e+0
Identity = 347/755 (45.96%), Postives = 473/755 (62.65%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIREDV-TIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEV---------------EVTNDEE----------DDLCYDEDDDE-----------DLDTGMTLKI------SSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDI-------------SSKA----GITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP+ AIP                     GTL A+ +T++SY+GYA M+G C L  ASG+  EY     +     VFS  NCT R C YG  ND Q +   S    L+Y GCFAATLSSAIASLVGAPRVLQALAKDKLYPFIG FS GHG NN+P RGY+L F I+  C+++  L+ ++ LLSNFF+AAYALINFSVFHAS+ KSPGWRPAF+YYN WVSLAGT LC+AVMFLM  + A IT  VV  LY Y+ YRKPE +WGSST+A  + + L+ + +L+   +HVKN+RP+++V +G P  R PL+DFA+L+TK LSLL+CADI +   + +  D++   A  WL++HK+KAF  +  + +FE G+ + M ++GLGK  PNMILMG+K +W   D    ++Y+N++H ALD  L++ ILR+  G D+S  +Q E  V +               E TN  E          D L  D D              + D   T K+       +I   K++K  + H+S   + + G  G  +P+ IL+++  F++K+  G+IDVWWLYDDGGL LL+P IL  R QF+ CQLR+F+L S  DDLD + +N+ +LL KFRIDY DVVII D+  +A E+T+ +F                +SKA     IT   LI  +E++NR++R+ E L +YS  S  +VM LP+PR  ++S+ +YM+WLE++TK +  P+L +RGNQ+SV+T YS
Sbjct:  177 LSGDLKDPSSAIP--------------------VGTLLAIFVTFLSYIGYAFMMGGCVLRTASGNVTEYLQVKGEPDEWAVFS--NCTDRSCLYGLHNDSQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSKGHGANNDPVRGYVLVFVISFGCILIAQLNAIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLAGTLLCIAVMFLMSWVMALITFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCADILKGTHSQRVHDALTRKAYHWLIRHKVKAFFNIVEDDSFEAGSQALMQISGLGKLRPNMILMGYKGNWRTCDREELKQYFNVIHNALDRYLAVGILRVQEGLDYSGVIQDEEFVPISKLDKKDAEEKMRRNESTNQLESQDVNITVLSDALSSDTDSPPVTPKMQHGGTVNCDPAATEKLLQSSTTVTITSKKMRKVSKNHKSSFPT-YVGPGGEPLPKSILNNITQFQRKQPKGLIDVWWLYDDGGLALLIPYILSTRSQFSGCQLRIFSLASKRDDLDKDQRNLAALLSKFRIDYSDVVIISDIHKKAMESTRKEFEIFIQPFRVKDEENFETSKAIEQTYITDSELIALREKTNRHMRIRELLLQYSKESTFIVMALPIPRKGVVSAPLYMAWLELLTKGM-PPYLIIRGNQTSVITVYS 907          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1228009299|ref|XP_021936562.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X1 [Zootermopsis nevadensis])

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 333/744 (44.76%), Postives = 472/744 (63.44%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI-REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTN--DEEDDLCYDEDDDEDLDTGMTLKISS----------------ILGDKL-----------KKSRQRHRSKRRSV--FTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF-----------------LDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP+ AIP                     GTL A+++T++SYVGY  M+G C L  ASG+  EY     +     +FS  NCT R C YG  ND Q +   S    L+Y GCFAATLSSAIASLVGAPRVLQALAKDKLYP IG FS GHG NN+P RGYIL F I+  C+++  L+V++ LLSNFF+AAYALINFSVFHAS+ KSPGWRPAF+YYN WVSL GT LC+AVMFLM  + A  T  VV  LY Y+ YRKPE +WGSST+A  + + L+ + +L+   +HVKN+RP+++V +G P  R PL+DFA+L+TK LSLL+CA I +   + +  +++   A +WL++HK+KAF  +  + +FE G    M ++GLGK  PNMILMG+K +W   D    ++Y+N++H ALD  L++ ILR+  G D+S  +Q E  V +   +  D E+ +  ++  ++ +   + + + S                +  D L           KKSR+  ++++ ++  +    G  +P+ IL+++  F++K+  G+IDVWWLYDDGGLTLL+P IL  R QF+ CQLR+F+L S  DDLD++ +N+ +LL KFRIDY DVVII D+  +A ++T+ +F                    S+K  IT   L+  +E++NR++R+ E L +YS  S  +VM LP+PR  ++S+ +YM+WLE++TK +  P L +RGNQ+SV+T YS
Sbjct:  352 LSGDLKDPSSAIP--------------------IGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTEYLQVKGEPNEWTIFS--NCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRTCDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDPVASDSLLESSTTVTVTSKKSRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPKGLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGM-PPCLIIRGNQTSVITVYS 1072          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1228009301|ref|XP_021936563.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X2 [Zootermopsis nevadensis])

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 333/744 (44.76%), Postives = 472/744 (63.44%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI-REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTN--DEEDDLCYDEDDDEDLDTGMTLKISS----------------ILGDKL-----------KKSRQRHRSKRRSV--FTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF-----------------LDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP+ AIP                     GTL A+++T++SYVGY  M+G C L  ASG+  EY     +     +FS  NCT R C YG  ND Q +   S    L+Y GCFAATLSSAIASLVGAPRVLQALAKDKLYP IG FS GHG NN+P RGYIL F I+  C+++  L+V++ LLSNFF+AAYALINFSVFHAS+ KSPGWRPAF+YYN WVSL GT LC+AVMFLM  + A  T  VV  LY Y+ YRKPE +WGSST+A  + + L+ + +L+   +HVKN+RP+++V +G P  R PL+DFA+L+TK LSLL+CA I +   + +  +++   A +WL++HK+KAF  +  + +FE G    M ++GLGK  PNMILMG+K +W   D    ++Y+N++H ALD  L++ ILR+  G D+S  +Q E  V +   +  D E+ +  ++  ++ +   + + + S                +  D L           KKSR+  ++++ ++  +    G  +P+ IL+++  F++K+  G+IDVWWLYDDGGLTLL+P IL  R QF+ CQLR+F+L S  DDLD++ +N+ +LL KFRIDY DVVII D+  +A ++T+ +F                    S+K  IT   L+  +E++NR++R+ E L +YS  S  +VM LP+PR  ++S+ +YM+WLE++TK +  P L +RGNQ+SV+T YS
Sbjct:  349 LSGDLKDPSSAIP--------------------IGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTEYLQVKGEPNEWTIFS--NCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRTCDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDPVASDSLLESSTTVTVTSKKSRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPKGLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGM-PPCLIIRGNQTSVITVYS 1069          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1228009303|ref|XP_021936564.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X3 [Zootermopsis nevadensis] >gi|646699945|gb|KDR10324.1| Bumetanide-sensitive sodium-(potassium)-chloride cotransporter [Zootermopsis nevadensis])

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 333/744 (44.76%), Postives = 472/744 (63.44%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI-REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTN--DEEDDLCYDEDDDEDLDTGMTLKISS----------------ILGDKL-----------KKSRQRHRSKRRSV--FTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF-----------------LDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP+ AIP G                    TL A+++T++SYVGY  M+G C L  ASG+  EY     +     +FS  NCT R C YG  ND Q +   S    L+Y GCFAATLSSAIASLVGAPRVLQALAKDKLYP IG FS GHG NN+P RGYIL F I+  C+++  L+V++ LLSNFF+AAYALINFSVFHAS+ KSPGWRPAF+YYN WVSL GT LC+AVMFLM  + A  T  VV  LY Y+ YRKPE +WGSST+A  + + L+ + +L+   +HVKN+RP+++V +G P  R PL+DFA+L+TK LSLL+CA I +   + +  +++   A +WL++HK+KAF  +  + +FE G    M ++GLGK  PNMILMG+K +W   D    ++Y+N++H ALD  L++ ILR+  G D+S  +Q E  V +   +  D E+ +  ++  ++ +   + + + S                +  D L           KKSR+  ++++ ++  +    G  +P+ IL+++  F++K+  G+IDVWWLYDDGGLTLL+P IL  R QF+ CQLR+F+L S  DDLD++ +N+ +LL KFRIDY DVVII D+  +A ++T+ +F                    S+K  IT   L+  +E++NR++R+ E L +YS  S  +VM LP+PR  ++S+ +YM+WLE++TK +  P L +RGNQ+SV+T YS
Sbjct:  222 LSGDLKDPSSAIPIG--------------------TLLAIVITFLSYVGYGFMMGGCVLRTASGNVTEYLQVKGEPNEWTIFS--NCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRTCDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDPVASDSLLESSTTVTVTSKKSRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPKGLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGM-PPCLIIRGNQTSVITVYS 942          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1061481000|gb|ODM98387.1| (Bumetanide-sensitive sodium-(potassium)-chloride cotransporter [Orchesella cincta])

HSP 1 Score: 644.81 bits (1662), Expect = 0.000e+0
Identity = 339/732 (46.31%), Postives = 465/732 (63.52%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATL-----EVFSFNNCT--FRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDW-LDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFS-------QHVQHECIVEVE-------VTNDEEDDLCYDEDDDEDLDTGMTLKI-----SSILGDKLKKSRQRHRSKRRS-VFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISS---------KAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDPA AIPKG                    TL A+ +TY++Y+ YA+M+G C L EA+G+  +Y   LN NAT      +   + +C    + C+YG  N  Q +  IS    L+Y GCFAATLSSAIASLVGAPRV QAL KDKLYP I  F+ G+G NN+P RGYIL F I+L C+++  L+ ++ LLSNFF+ AY LINFS FHA++ KSPGWRPAF+YYN WVSL G  LC+AVMFLM  +TA IT+A V  LY Y+ YRKPE +WGSST+AQ + + L+++ DL++  +HVKNYRP+++V SG PS R PL+DFA+L+ K LSL++CA  ++     K ++++   A  WL KHK K F+ +  +++FE G  S M + GLGK  PN +LMG+KTDW   +    + Y+N++H+ALDM L + ILR+  G D++       Q    + +++++       V N  E  L  D    E      + KI     +   G+     ++R R    S ++ G NG A+P+ ++  L  F++K+K G IDVWWLYDDGGLTLLLP IL  R QF+ C LR+F+L S  D+L  E +NM +LL KFRIDY DV++IPDVT +A E T+ +F  + +            +T   LI  +E++NR+LRL E L  +S  S  +VMTLPMPR   +S+ +YM+WLE+MTK +  PFL +RGNQ+SVLTFYS
Sbjct:  282 LSGDLKDPASAIPKG--------------------TLLAITITYITYILYAVMMGGCALREATGNETQY--ILNMNATFMEENPDFLLYTDCKDGLQTCDYGMLNSAQVMELISSWGPLIYMGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYILVFIISLGCLLIAELNAIAPLLSNFFLMAYFLINFSCFHATVTKSPGWRPAFKYYNAWVSLVGCVLCIAVMFLMSWVTALITVACVIFLYLYVAYRKPEVNWGSSTQAQSYNSALKSIQDLNVVEEHVKNYRPQLLVLSGMPSSRPPLIDFANLIVKNLSLMLCAQVVKGSYNQKVRNALSRQAYNWLAKHKKKGFYTMIEDESFERGCRSIMQICGLGKLRPNTVLMGYKTDWQRGNRQELKEYFNVIHEALDMYLGVAILRVQEGLDYTGVIEDEDQATPQQILLDMKKDKEKGIVRNQSESQLSQDGTSSEASSPPSSPKIERSNPAGPTGETAVGLKKRQRKHSLSALYAGPNGSALPRDVISHLTQFQRKQKKGTIDVWWLYDDGGLTLLLPYILTSRSQFSGCALRIFSLASKKDELGREQRNMAALLSKFRIDYSDVIVIPDVTKKAHENTKKEFDAVIAPFKSNNSDDGTSVTEAELIACREKTNRHLRLRELLLTHSKESTFIVMTLPMPRKGTVSAPLYMAWLEMMTKGMP-PFLMIRGNQTSVLTFYS 990          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1045308339|gb|ANU78813.1| (Na+/K+/2Cl- cotransporter [Portunus trituberculatus])

HSP 1 Score: 637.106 bits (1642), Expect = 0.000e+0
Identity = 327/733 (44.61%), Postives = 472/733 (64.39%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTF--------RKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECI---------VEVEVTNDE-----EDDLCYDEDDDEDLDTGMTLKI--SSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF----LDISSKAG---------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDPA AIPKG                    TL+A++ T+ +Y+ Y IMIG+  L +A+GD   Y  + N +   E  +F NC+         + C +G  N  Q +  +S    L+YAGCFAATLSSAIASLVGAPRVLQALAKDKLYP I +FS G G NN+P RGYIL F I+  C+M+G+L+VVS+LLSNFF+A+Y+LINFS FHAS++KSPGWRP+F+YYN W+S  G  LC+ VMFL+D +TA  T  ++  LY ++ YR P  +WGSST+AQ +++ L+   DL+   +HVKNYRP+I+V +G    R PL+DFA+ +TK +SLL C   I+   T + ++S+   +  WL++H+++AF+ +    N EDGA +   L GLGK  PN +++G+K +W   D M  + Y+N LH+ALDM   L ILR+P+G D+SQ ++ E              + T D+      + L  D  D E      + ++  S++  D   ++ ++ R+   ++F G  G  + + +L+++ +FK+K+K G IDVWWLYDDGGLTLL+P IL  R Q++ C+LRVFAL +  D+LD E ++M +LL KFRIDY DV++IPDV  +A+E+++ +F     D  +K           I+   L+  +E++NR++RL E L E S +S +VVMTLPMPR + +S+ +YM+WLE +T+D+  PFL +RGNQ+SVLTFYS
Sbjct:  345 LSGDLKDPAEAIPKG--------------------TLAAIVTTFCTYIIYPIMIGAAVLRDATGDVNVYQMYQNHSID-ENPAFTNCSTTGHVDNGTQVCPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYILVFVISFICIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWLGGILCLIVMFLIDWITALATFLIIVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNTIEEHVKNYRPQILVLTGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLIRHRIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPMELKAYFNTLHEALDMYFGLVILRVPQGLDYSQIIEDEDSPITMNGNEGAITQTTGDKPGQSTANQLTQDGTDSEASSPPGSPQVERSTVATDANTENSKKRRTSLANLFRGPGGSELSKDVLNNITMFKRKQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKTKDEVDKENDGLLISEAELLGQREKTNRHIRLRELLLENSRDSSLVVMTLPMPRKTSVSAPLYMAWLETLTRDM-PPFLLIRGNQTSVLTFYS 1055          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1036124418|gb|ANJ60741.1| (Na+/K+/2Cl- cotransporter [Scylla paramamosain])

HSP 1 Score: 632.869 bits (1631), Expect = 0.000e+0
Identity = 328/733 (44.75%), Postives = 470/733 (64.12%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTF--------RKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIV------EVEVTNDEED--------DLCYDEDDDEDLDTGMTLKI--SSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF----LDISSKAGITTD----MLIE-----NQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDPA AIPKG                    TL+A++ T+ +Y+ Y IMIG+  L +A+GD   Y  + N +   E  +F NC+         + C +G  N  Q +  +S    L+YAGCFAATLSSAIASLVGAPRVLQALAKDKLYP I +FS G G NN+P RGY+L F I+  C+M+G+L+VVS+LLSNFF+A+Y+LINFS FHAS++KSPGWRP+F+YYN W+S  G  LC+ VMFL+D +TA IT  +   LY ++ YR P  +WGSST+AQ +++ L+   DL+   +HVKNYRP+I+V SG    R PL+DFA+ +TK +SLL C   I+   T + ++S+   +  WL +H ++AF+ +    N EDGA +   L GLGK  PN +++G+K +W   D +  + Y+N LH+ALDM   + ILR+P+G D+SQ ++ E         E  +T   ED         L  D  D E      + ++  S+   D   ++ ++ R+   ++F G  G  + + +L+++ +FK+K+K G IDVWWLYDDGGLTLL+P IL  R Q++ C+LRVFAL +  D+LD E ++M +LL KFRIDY DV++IPDV  +A+E+++ +F     D  +K  +  +    M+ E      +E++NR++RL E L E S +S +VVMTLPMPR + +S+ +YM+WLE +T+D+  PFL +RGNQ+SVLTFYS
Sbjct:  343 LSGDLKDPAEAIPKG--------------------TLAAILTTFCTYIIYPIMIGAAVLRDATGDKDVYVMYQNLSID-ENPAFTNCSLIGKVDNGTQVCPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYVLVFVISFVCIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWVGGILCLIVMFLIDWVTALITFLITVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNSIEEHVKNYRPQILVLSGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLTRHSIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPLELKAYFNTLHEALDMYFGVVILRVPQGLDYSQIIEDEDSPITMNGNESAITQSPEDKPGQSTANQLTQDGTDSEASSPPGSPQVERSAATTDANGENSKKRRTSMANLFRGPGGTELSKDVLNNITMFKRKQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFRTKDEVDKENDGLMISEAEFLGQREKTNRHIRLRELLLENSRDSSLVVMTLPMPRKTSVSAPLYMAWLETLTRDM-PPFLLIRGNQTSVLTFYS 1053