kunitz-like protease inhibitor precursor, maker-scaffold580_size130538-snap-gene-0.29 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000005801 (supercontig:LSalAtl2s:LSalAtl2s312:520364:527173:1 gene:EMLSAG00000005801 transcript:EMLSAT00000005801 description:"maker-LSalAtl2s312-augustus-gene-5.18") HSP 1 Score: 217.238 bits (552), Expect = 6.637e-60 Identity = 165/550 (30.00%), Postives = 247/550 (44.91%), Query Frame = 0
Query: 170 KLLRIESWPGRR--NNSFIVMNDLVVLQ-----------------TNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH-KPEKDDPCISPLLKG--SCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIF----------RVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCE-IQIAPRCRIEHTFEPCLPSPSY----TSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALE---GCLLRPERGS--CHGSLPRFYYEPMDETCHSFTFSGCH-GNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQP---RFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVAEIFIGIALSGLVILGVYFGFR 673
++LRIESW G N+ ++++D + Q T + + T STT +P+T S + PC L M GPC IR++++ +EKC F +GGCKGN N FK+ ++C + C K + D C P G CS E++ FD Q TC F+YSGCGGN NNF S EC++ C +D S+ + F + P R +CLKP G C + ++ +N CV +IY GCGG+DN F + + C+ C+ ++ +E +P T+ + Y + C GC + K G F + + C ++C S S V P + C GS C+ SL RFY++ TC F ++GC NGN F + C A C + S C PK G + D R++++ ETC F + GC G++NNF C +C N ++ I A + ST S VAE+ +G+ L L +G+ G +
Sbjct: 425 QILRIESWKGSALDNSEVVILDDFSLKQELLQHELPSSSTPKVPKTTKVTSLDELTTASTTINVPTTLLQEITSFKSDADDNFPCDLPMNSGPCRGNFIRYYFDTASEKCLEFSYGGCKGNANRFKAKKDCYSLCPHRDKSKAIDSCKLPRDPGDVKCSFYSERYFFDSQLGTCLIFLYSGCGGNSNNFKSRKECKNKCS--VDEDSRSIAKKTFLRLTGSVKRDKFPSR--QECLKPAEVGPCGGNYVAFAYDLNKNDCVRFIYGGCGGNDNRFYTIDKCRTVCQKKKVKNHLDLEDPCNDIIPKQKKKQHCTNRYFYDPRSKRCY--SGCPSLKVKG--FVSRASCFKSCAPASSVSTTVMPELLITPNVKCTNPLSTGSNLCNDSLIRFYFDQSTNTCEKFIYTGCGVRNGNIFPRKEVCAAVCRVP--------VGEGSPCYSPKDVGSSKCDAAKSIRYYFDFWKETCLPFEFLGCNGNKNNFVDFQSCNTSC-NFIINESIPSSAPDMSI----STISAPTVAEVVLGVILIILFTIGIVMGLK 953 HSP 2 Score: 51.6026 bits (122), Expect = 6.369e-7 Identity = 22/47 (46.81%), Postives = 27/47 (57.45%), Query Frame = 0
Query: 26 PERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMC 72
P C FY RYFF+ G C F +SGCG N NNF+ +K C+ C
Sbjct: 577 PGDVKCSFYSERYFFDSQLGTCLIFLYSGCGGNSNNFKSRKECKNKC 623
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000007668 (supercontig:LSalAtl2s:LSalAtl2s446:196154:280179:1 gene:EMLSAG00000007668 transcript:EMLSAT00000007668 description:"maker-LSalAtl2s446-snap-gene-2.16") HSP 1 Score: 183.726 bits (465), Expect = 2.748e-48 Identity = 140/487 (28.75%), Postives = 212/487 (43.53%), Query Frame = 0
Query: 179 GRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISP--------------LLK--GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD-CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQI----------APRCRIEHTFEPC-LPSPS---YTSV-WVY---SEEQRMCVVTVGCSNTKRIGNEFSTSSECERTC-----LSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC----HDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
G N+F +++ NQ +P+ D T LP + + + C+L+ G C L R++Y+ + KC F + GC GN NNFK+L EC++ C + + + P + P L K G C ++++D S C +F Y+GCGGN NNF +L EC+ C+ N L P R++ + C P +G C Y ++ +C+ + Y+GCGG+ N F + CKN CE Q P C LP S Y + W Y S + T C + N F T ECE C +SD ++L F ++ C L + G C ++ R+YY+ C F ++GC GN NNF+ Q C C HD N ++C K KG C + R++++ + C++F Y GC G+RNNF C+ C
Sbjct: 381 GGNQNNFKTISECKSYCKNQYYKPNI--HDEKVTPLPPSKDVNEV-----------CRLSKEAGHCYNLLWRYYYDSYSGKCLRFTYTGCGGNRNNFKTLPECKSYCQKQYYKPNIPLVKPRPLPPLRDVIDVCRLSKETGHCYDKLWRYYYDSYSGKCLRFTYTGCGGNQNNFKTLPECKRYCEKQYYKPHNPLVHSQSLPPSRNVINVCGLPKEAGHCYNLLWRYHYDSYSGKCLKFSYTGCGGNQNNFKTVTDCKNYCEKQYYKPPTHLVKPKPLTHPSGVINVCGLPKESGHCYNKLMWRYYYDSYYGKCLKFTFSCGGNQ---NNFKTLHECENYCKVKHYMSD--NTLVASNPFYVKSCHLPKKTGHCFRNIWRYYYDSTIGKCEKFIYTGCGGNLNNFERLQDCQKVCIAHVHDLPKVTPTPPEPNTNVCLLSKEKGHCFNNVWRYYFDKKTQKCQQFHYSGCGGNRNNFKIITDCKKRC 849 HSP 2 Score: 180.644 bits (457), Expect = 2.792e-47 Identity = 179/664 (26.96%), Postives = 274/664 (41.27%), Query Frame = 0
Query: 18 IPEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEE-EILRQEPRRSLKNLTLDFDQGFEDWDNYD--WQII--SYNSDLAR--------GLNLYSPDPG-SRSCA----GPLRPQGFSKSHPKLMHPIQV--DRNEAITITLSLKLLRIESWPGR-----------RNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPC-DLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCIS-----------PLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPS----YTSVWVY----SEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFA---LEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILT----NASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
+ E C L E GHC+ RY+++ SG C F+++GCG N NNF+ C+ C +++ P L+++ E YD W+ SY+ R N + P R C P P S+S P + I V EA L +S+ G+ N+F + D Q +P T P T P+ ++ C L G C + + R++Y+ KC F F C GN NNFK+L ECE C D+ ++ P G C + ++++D C KF+Y+GCGGN NNF+ L +CQ C H ++ L + P+ + + CL G C Y F++ +C + YSGCGG+ N F CK RCE+++ P + CL S + S+W Y S ++ + GC GN F +C+ C + V+P + C L E+G C+ + R+Y++ C F ++G GNGNNF+ C C + + T + + C PK KG C + R++Y+ +I C++F Y GC G+ NNF CE C +
Sbjct: 420 VNEVCRLSKEAGHCYNLLWRYYYDSYSGKCLRFTYTGCGGNRNNFKTLPECKSYCQKQYYKPNIPLVKPRPLPPLRDVIDVCRLSKETGHCYDKLWRYYYDSYSGKCLRFTYTGCGGNQNNFKTLPECKRYCEKQYYKPHNPLVHSQSLPPSRNVINVCGLPKEAGHCYNLLWRYHYDSYSGKCLKFSYTGCGGNQNNFKTVTDCKNYCEKQYYKPPT----HLVKPKPLTHPSGVINV---------CGLPKESGHCYNKLMWRYYYDSYYGKCLKFTF-SCGGNQNNFKTLHECENYCKVKHYMSDNTLVASNPFYVKSCHLPKKTGHCFRNIWRYYYDSTIGKCEKFIYTGCGGNLNNFERLQDCQKVCIAH----VHDLPKVTPTPPEPNTNVCLLSKEKGHCFNNVWRYYFDKKTQKCQQFHYSGCGGNRNNFKIITDCKKRCEVEVTPT-PPKQDINVCLLSKVRGNCHKSIWRYYFDKSTQKCRKFIYSGCGEN---GNNFKRIIDCKVRC------EVTVKPTTPNPNINVCFLSKEQGRCYKRIWRYYFDKKTHRCEKFVYTGXGGNGNNFKRXIDCNKRCDVQQTQPPIVTPTPPKSDYNSCYLPKEKGRCSKSIWRYYYDSNIGKCKKFIYTGCGGNLNNFERLQDCENVCTD 1055 HSP 3 Score: 174.096 bits (440), Expect = 3.765e-45 Identity = 133/467 (28.48%), Postives = 211/467 (45.18%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSE--HKPEKDDPCISPLL--------------KGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC-KDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ-IAPRCRIEH--TFEPC--------LPSPS---YTSVWVYSEEQR----MCVVTVGCSNTKRIGNEFSTSSECERTC----LSDRSHSLEVEPMFALEG----CLLRPERGSCHGSLP-RFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTN----ASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVAE 653
C+L+ G C L R++Y+ + KC F + GC GN NNFK++ EC++ C +KP D ++PL G C ++++D S C +F Y+GCGGN NNF +L EC+S C K + P + + + + C +G C + Y ++ +C+ + Y+GCGG+ N F + CK CE Q P + H + P LP + Y +W Y + + GC + N F T ++C+ C +H ++ +P+ G C L E G C+ L R+YY+ C FTFS C GN NNF++ C C +D +++ + C PK G C R+ R++Y+ +I CE+F Y GC G+ NNF C+ C+ A + L + + T E +T+ ++ E
Sbjct: 347 CRLSKETGHCYDKLWRYYYDSYSGKCIRFTYTGCGGNQNNFKTISECKSYCKNQYYKPNIHDEKVTPLPPSKDVNEVCRLSKEAGHCYNLLWRYYYDSYSGKCLRFTYTGCGGNRNNFKTLPECKSYCQKQYYKPNIPLVKPRPLPPLRDVIDVCRLSKETGHCYDKLWRYYYDSYSGKCLRFTYTGCGGNQNNFKTLPECKRYCEKQYYKPHNPLVHSQSLPPSRNVINVCGLPKEAGHCYNLLWRYHYDSYSGKCLKFSYTGCGGNQ---NNFKTVTDCKNYCEKQYYKPPTHLVKPKPLTHPSGVINVCGLPKESGHCYNKLMWRYYYDSYYGKCLKFTFS-CGGNQNNFKTLHECENYCKVKHYMSDNTLVASNPFYVKSCHLPKKTGHCFRNIWRYYYDSTIGKCEKFIYTGCGGNLNNFERLQDCQKVCI-----AHVHDLPKVTPTPPEPNTNVCLLSKE 804 HSP 4 Score: 166.007 bits (419), Expect = 1.596e-42 Identity = 170/661 (25.72%), Postives = 258/661 (39.03%), Query Frame = 0
Query: 19 PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCS-------------EHKPEKDD---PCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD-HMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ--------IAPR-----------CRIEHTFEPCLPSPSYTSVWVYSEEQR----MCVVTVGCSNTKRIGNEFSTSSECERTCLSDR-------SHSLEVEP-MFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSI-----LTNAS----ICEQPKLKGPC-RRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
P CLL G C Y RY+FN + +CE F +SGC N NNF+ K C C +L +P + + +D I+S +S L S R P PK++ V++ I L +P F ++ L T+ + P P L+ S G C L G C L R++++ + KC F++ GC GN NNFK+L C+ C +H P D C G C ++++D S C +F Y+GCGGN NNF +++EC+S CK+ + P ++ K C +G C Y ++ +C+ + Y+GCGG+ N F + CK+ C+ Q + PR CR+ C Y +W Y + + GC + N F T EC+R C HS + P + C L E G C+ L R++Y+ C F+++GC GN NNF++ C C + + LT+ S +C PK G C + R++Y+ C +F + C G++NNF T +CE C
Sbjct: 94 PPTCLLPKVTGPCEGYFERYYFNPDTDSCEGFIYSGCRGNANNFQSYKDCCVTC--------HVLSSKPEGKSIDFIEPSSKFLDDSSVAKNGIVSSHS-----LKFTSLVSQWRKTHSPF---------PKIL--AHVNKGPIIHFPL---------YPNIHRRPF--LHKLHFKPTHPQIHPRLPGYHPHL----HPKPKLVLAYGHSYTKGV-CHLPKKAGNCYNKLRRYYFDSYSGKCLKFIYTGCGGNQNNFKTLYMCKNHCKNRHYTVNIPLVKRKHLPSSRDVINVCRLSKETGHCYDKLWRYYYDSYSGKCIRFTYTGCGGNQNNFKTISECKSYCKNQYYKPNIHDEKVTPLPPSKDVNEVCRLSKEAGHCYNLLWRYYYDSYSGKCLRFTYTGCGGNRNNFKTLPECKSYCQKQYYKPNIPLVKPRPLPPLRDVIDVCRLSKETGHC-----YDKLWRYYYDSYSGKCLRFTYTGCGGNQ---NNFKTLPECKRYCEKQYYKPHNPLVHSQSLPPSRNVINVCGLPKEAGHCYNLLWRYHYDSYSGKCLKFSYTGCGGNQNNFKTVTDCKNYCEKQYYKPPTHLVKPKPLTHPSGVINVCGLPKESGHCYNKLMWRYYYDSYYGKCLKFTF-SCGGNQNNFKTLHECENYC 705 HSP 5 Score: 150.599 bits (379), Expect = 1.209e-37 Identity = 118/415 (28.43%), Postives = 186/415 (44.82%), Query Frame = 0
Query: 222 TLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHK---------PEKDD--PCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPC---LPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEP-MFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
T+ +T + C L+ +G C + R++++ KT +CE FV+ G GNGNNFK +C C + P K D C P KG CS S ++++D C KF+Y+GCGGN NNF+ L +C++ C DH+ + + P + T + + E+ + + +PY+ S + ++ +++ +I I C+ E T+ C L Y S+ E + GC+ N F + S C+R C S ++ + GC L E G C+ L R+Y++ C F ++GC GN NNF S++ C C GS +C K G C ++F+N CE+F Y GC G++NNF +C TC
Sbjct: 918 TVKPTTPNPNINVCFLSKEQGRCYKRIWRYYFDKKTHRCEKFVYTGXGGNGNNFKRXIDCNKRCDVQQTQPPIVTPTPPKSDYNSCYLPKEKGRCSKSIWRYYYDSNIGKCKKFIYTGCGGNLNNFERLQDCENVCTDHIPKY-----KEVTPTPPKVEYSLEDKDTGKNSEGEKSMV-------ELLPYLESL---HKIYYGGNKGGQHKSQITIDWACKFEKTYGTCSQELKRYFYNSITSKCES----FIYTGCAGNP---NNFLSQSRCDRICAKSISQKTVLQSGKYIFPGCYLGREVGYCNSYLERYYFDTNLGRCVQFIYTGCGGNTNNFNSYKKCNFLC-------GGSAERPIKVCRLEKNIGYCHDQIDKYFFNTHTRKCEKFIYSGCGGNKNNFDNETECNETC 1303 HSP 6 Score: 147.902 bits (372), Expect = 9.871e-37 Identity = 120/480 (25.00%), Postives = 197/480 (41.04%), Query Frame = 0
Query: 214 LPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC----SEHKPEKD-DPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ---------IAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC----VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPM-----FALE---------------------------------------------GCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASI----CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
LP TP + E + C L+ +G C + R++++ KT+KC+ F + GC GN NNFK + +C+ C + P++D + C+ ++G+C S +++FD + C KF+YSGCG NGNNF + +C+ C+ + PT P +++ C G C Y F++ ++C ++Y+G GG+ N F C RC++Q P+ + P S+W Y + + + GC N F +CE C EV P ++LE C G+C L R++Y + C SF ++GC GN NNF S C C A+ + ++L + C + G C R++++ ++ C +F Y GC G+ NNF + +C C
Sbjct: 784 LPKVTP------TPPEPNTNVCLLSKEKGHCFNNVWRYYFDKKTQKCQQFHYSGCGGNRNNFKIITDCKKRCEVEVTPTPPKQDINVCLLSKVRGNCHKSIWRYYFDKSTQKCRKFIYSGCGENGNNFKRIIDCKVRCEVTVKPT----------TPNPNINVCFLSKEQGRCYKRIWRYYFDKKTHRCEKFVYTGXGGNGNNFKRXIDCNKRCDVQQTQPPIVTPTPPKSDYNSCYLPKEKGRCSKSIWRYYYDSNIGKCKKFIYTGCGGNL---NNFERLQDCENVCTDHIPKYKEVTPTPPKVEYSLEDKDTGKNSEGEKSMVELLPYLESLHKIYYGGNKGGQHKSQITIDWACKFEKTYGTCSQELKRYFYNSITSKCESFIYTGCAGNPNNFLSQSRCDRIC--AKSISQKTVLQSGKYIFPGCYLGREVGYCNSYLERYYFDTNLGRCVQFIYTGCGGNTNNFNSYKKCNFLC 1242 HSP 7 Score: 105.145 bits (261), Expect = 2.501e-23 Identity = 90/357 (25.21%), Postives = 141/357 (39.50%), Query Frame = 0
Query: 208 DSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIE---HTFEPCLPS------PSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
D T P TP S S C L +G C + R++Y+ KC+ F++ GC GN NNF+ L++CE C++H P+ + +P K + + K G + + L +S K + N G+H ++ C T G C E Y +N ++C +IY+GC G+ N FLS C C I+ + ++ + F C SY + + CV + GC N F++ +C C ++V C L G CH + ++++ C F +SGC GN NNF + C TC
Sbjct: 982 DVQQTQPPIVTPTPPKSDYNS------CYLPKEKGRCSKSIWRYYYDSNIGKCKKFIYTGCGGNLNNFERLQDCENVCTDHIPKYKEVTPTP----------PKVEYSLEDKDTGK----NSEGEKSMVELLPYLESLHKIYY--GGNKGGQHKSQITIDWA--CKFEKTYGTCSQELKRYFYNSITSKCESFIYTGCAGNPNNFLSQSRCDRICAKSISQKTVLQSGKYIFPGCYLGREVGYCNSYLERYYFDTNLGRCVQFIYTGCGGNT---NNFNSYKKCNFLCGGSAERPIKV--------CRLEKNIGYCHDQIDKYFFNTHTRKCEKFIYSGCGGNKNNFDNETECNETC 1303 HSP 8 Score: 92.8189 bits (229), Expect = 1.630e-19 Identity = 47/136 (34.56%), Postives = 68/136 (50.00%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLS 370
C L G C+ YL R++++ +C F++ GC GN NNF S ++C C C G C +K+ F+ + C KF+YSGCGGN NNFD+ EC TC +N G + VP++ L+
Sbjct: 1192 CYLGREVGYCNSYLERYYFDTNLGRCVQFIYTGCGGNTNNFNSYKKCNFLCGGSAERPIKVCRLEKNIGYCHDQIDKYFFNTHTRKCEKFIYSGCGGNKNNFDNETECNETCTQDKHGLINEPGHGLISVPEKGLN 1327 HSP 9 Score: 89.7373 bits (221), Expect = 1.497e-18 Identity = 46/130 (35.38%), Postives = 74/130 (56.92%), Query Frame = 0
Query: 226 STSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC-------SEHKPEKDDP--CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
+ ++ + C A+ G C+ ++++N +T +CE FV+GGCKG+ N F SL +C+T C S ++ P C+ P + G C G E+++F+ + +C F+YSGC GN NNF S +C TC
Sbjct: 18 AATKTSSPKCNEALDIGECNQNETKYYFNNETNECEEFVYGGCKGSENRFDSLSDCKTLCESKDQNSSAYRQWGSYPPTCLLPKVTGPCEGYFERYYFNPDTDSCEGFIYSGCRGNANNFQSYKDCCVTC 147 HSP 10 Score: 77.411 bits (189), Expect = 8.873e-15 Identity = 65/222 (29.28%), Postives = 97/222 (43.69%), Query Frame = 0
Query: 303 GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC-KDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC------EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGS 515
G+CS +++ ++ + C F+Y+GC GN NNF S + C C K T+ G++IF C G C Y F+ +CV +IY+GCGG+ N F S++ C C I++ CR+E C + ++ R C + GC K N F +EC TC D+ H L EP L + PE+G G+
Sbjct: 1129 GTCSQELKRYFYNSITSKCESFIYTGCAGNPNNFLSQSRCDRICAKSISQKTVLQSGKYIF-------PGCYLGREVGYCNSYLERYYFDTNLGRCVQFIYTGCGGNTNNFNSYKKCNFLCGGSAERPIKV---CRLEKNIGYCHDQ---IDKYFFNTHTRKCEKFIYSGCGGNK---NNFDNETECNETCTQDK-HGLINEPGHGL---ISVPEKGLNSGT 1330 HSP 11 Score: 73.9442 bits (180), Expect = 1.175e-13 Identity = 42/148 (28.38%), Postives = 67/148 (45.27%), Query Frame = 0
Query: 510 GSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCH--DAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL---NREVDAFIQFLAEESETFHEESTSSGVVVA 652
G C+ + ++Y+ C F + GC G+ N F S C C D A + C PK+ GPC R+++NP ++CE F Y GC+G+ NNF + C TC ++ I F+ S+ + S + +V+
Sbjct: 34 GECNQNETKYYFNNETNECEEFVYGGCKGSENRFDSLSDCKTLCESKDQNSSAYRQWGSYPPTCLLPKVTGPCEGYFERYYFNPDTDSCEGFIYSGCRGNANNFQSYKDCCVTCHVLSSKPEGKSIDFIEPSSKFLDDSSVAKNGIVS 181 HSP 12 Score: 71.633 bits (174), Expect = 6.349e-13 Identity = 37/131 (28.24%), Postives = 63/131 (48.09%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNA---------SICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNR 624
C L + G+C+ L R+Y++ C F ++GC GN NNF++ C C + + ++ ++C K G C R++Y+ C F Y GC G++NNF T ++C++ C N+
Sbjct: 270 CHLPKKAGNCYNKLRRYYFDSYSGKCLKFIYTGCGGNQNNFKTLYMCKNHCKNRHYTVNIPLVKRKHLPSSRDVINVCRLSKETGHCYDKLWRYYYDSYSGKCIRFTYTGCGGNQNNFKTISECKSYCKNQ 400 HSP 13 Score: 70.4774 bits (171), Expect = 1.203e-12 Identity = 43/131 (32.82%), Postives = 66/131 (50.38%), Query Frame = 0
Query: 296 CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC--KDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEI 424
C L G C+ + K++F+ +++ C +FVY GC G+ N FDSL++C++ C KD G + CL P +G C+ Y FN + C +IYSGC G+ N F S++ C C +
Sbjct: 27 CNEALDIGECNQNETKYYFNNETNECEEFVYGGCKGSENRFDSLSDCKTLCESKDQNSSAYRQWGSYP--------PTCLLPKVTGPCEGYFERYYFNPDTDSCEGFIYSGCRGNANNFQSYKDCCVTCHV 149 HSP 14 Score: 66.6254 bits (161), Expect = 2.119e-11 Identity = 39/120 (32.50%), Postives = 60/120 (50.00%), Query Frame = 0
Query: 449 WVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLS-DRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSIL 565
+ ++ E C V GC ++ N F + S+C+ C S D++ S + CLL G C G R+Y+ P ++C F +SGC GN NNFQS++ C TCH + +G +
Sbjct: 43 YYFNNETNECEEFVYGGCKGSE---NRFDSLSDCKTLCESKDQNSSAYRQWGSYPPTCLLPKVTGPCEGYFERYYFNPDTDSCEGFIYSGCRGNANNFQSYKDCCVTCHVLSSKPEGKSI 159 HSP 15 Score: 59.6918 bits (143), Expect = 2.656e-9 Identity = 76/325 (23.38%), Postives = 111/325 (34.15%), Query Frame = 0
Query: 366 KRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ------------IAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTC--LSDR----------------------------SHSLEVEPMFA--------LEGCLLRPERGS-CHGSL-PRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEE 636
K S C + + G+C + Y FN N+C ++Y GC GS+N F S CK CE + P C + PC Y + ++ + C + GC N F + +C TC LS + SHSL+ + + L +G H L P + P H F H + H V A G T +C PK G C R++++ C +F Y GC G++NNF T C+ C NR I + +
Sbjct: 21 KTSSPKCNEALDIGECNQNETKYYFNNETNECEEFVYGGCKGSENRFDSLSDCKTLCESKDQNSSAYRQWGSYPPTCLLPKVTGPC---EGYFERYYFNPDTDSCEGFIYSGCRGN---ANNFQSYKDCCVTCHVLSSKPEGKSIDFIEPSSKFLDDSSVAKNGIVSSHSLKFTSLVSQWRKTHSPFPKILAHVNKGPIIHFPLYPNIHRRPF---LHKLHFKPTHPQIHPRLPGYHPHLHPKPKLVLAYGHSYTKG-VCHLPKKAGNCYNKLRRYYFDSYSGKCLKFIYTGCGGNQNNFKTLYMCKNHCKNRHYTVNIPLVKRK 335 HSP 16 Score: 53.1434 bits (126), Expect = 2.461e-7 Identity = 27/79 (34.18%), Postives = 40/79 (50.63%), Query Frame = 0
Query: 211 TTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHK 289
T S + L ++E C+L G C + ++F+N T KCE F++ GC GN NNF + EC TC++ K
Sbjct: 1229 TNNFNSYKKCNFLCGGSAERPIKVCRLEKNIGYCHDQIDKYFFNTHTRKCEKFIYSGCGGNKNNFDNETECNETCTQDK 1307
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000007907 (supercontig:LSalAtl2s:LSalAtl2s467:26502:36182:-1 gene:EMLSAG00000007907 transcript:EMLSAT00000007907 description:"maker-LSalAtl2s467-augustus-gene-0.15") HSP 1 Score: 150.984 bits (380), Expect = 1.038e-37 Identity = 112/394 (28.43%), Postives = 173/394 (43.91%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCL--KPITSGDCQ--LEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
C L GPC L +++++ ++C F +GGC GN N F LE C+ +C + + D C P + G+C E+F+FD F Y GC GN NNF S+ CQS C S+ I + L CL K + D Q EQ + ++ ++ C + G G+ N FL+ + C+ C + C + PC + S W + E++ C GC GN F + CE C D + ++ C L + G C +P +Y+ + C +F +SGC GN N F + + C C I ++C GPC +++++ TC EF +GGC+G+ N F + +CE CL
Sbjct: 1269 CLLPRDPGPCKDNLPKWYFDNFEKRCVPFYYGGCDGNDNKFDDLESCQKSCPKEFLQAD-VCKLPQVPGTCGDYLERFYFDIDEGVXKPFYYGGCDGNKNNFKSMENCQSRC---------SVDYSIPIAQEFKLEFCLLSKDVGVVDEQNPKEQNRWYYDSSDGVCKQMQFKGRRGNGNRFLTRQDCEASCR-ESQNICTLPKVRGPC--NSQIDSFW-FDEDKNSCFKFTWGGCQGN---GNRFESMEFCEAKC-KDTGSGAPSKEKANVDICNLPIDSGPCTDRIPNWYFNTKNGKCSAFIYSGCEGNANRFITQEQCDRNC---------GIFKGKNVCGSQMDIGPCVGRFKKWYFDLHTRTCHEFTFGGCEGNGNRFSSLGECETVCL 1635 HSP 2 Score: 147.132 bits (370), Expect = 1.533e-36 Identity = 113/397 (28.46%), Postives = 159/397 (40.05%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPS----------PSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C L +GPC+ Y R+ YN G N + C+ P G C + K++FD C F Y GC GN N FD L CQ +C L + ++P+ P T GD LE+ + F+ E P+ Y GC G+ N F S E C++RC + + E E CL S P + W Y +C + + GN F T +CE +C ++ C L RG C+ + F+++ +C FT+ GC GNGN F+S + C A C D A N IC P GPC P +++N C F Y GC+G+ N F T+ QC+ C
Sbjct: 1241 CVLPEVKGPCEGYYPRYSYN---------------SEGFNRQM------------------CLLPRDPGPCKDNLPKWYFDNFEKRCVPFYYGGCDGNDNKFDDLESCQKSCPKEF------LQADVCKLPQ-------VPGTCGD-YLER--FYFDIDEGVXKPFYYGGCDGNKNNFKSMENCQSRCSVDYSIPIAQEFKLEFCLLSKDVGVVDEQNPKEQNRWYYDSSDGVCK-QMQFKGRRGNGNRFLTRQDCEASCRESQNI------------CTLPKVRGPCNSQIDSFWFDEDKNSCFKFTWGGCQGNGNRFESMEFCEAKCKDTGSGAPSKEKANVDICNLPIDSGPCTDRIPNWYFNTKNGKCSAFIYSGCEGNANRFITQEQCDRNC 1575 HSP 3 Score: 139.428 bits (350), Expect = 4.441e-34 Identity = 107/417 (25.66%), Postives = 172/417 (41.25%), Query Frame = 0
Query: 230 LAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH------KPEKDDPCISPLLKGSCSGSGEK----FHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC----------EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC---HDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
L C+L G C YL RF+++ + F +GGC GN NNFKS+E C++ CS + K + C+ G K +++D C + + G GNGN F + +C+++C++ S + C P G C + + F++ +N C + + GC G+ N F S E C+ +C E C + PC W ++ + C + GC N F T +C+R C + ++ C + + G C G ++Y++ TCH FTF GC GNGN F S C C + ++ + + ++ + IC +G C R+++N C F Y GC G+RN F + C C
Sbjct: 1324 LQADVCKLPQVPGTCGDYLERFYFDIDEGVXKPFYYGGCDGNKNNFKSMENCQSRCSVDYSIPIAQEFKLEFCLLSKDVGVVDEQNPKEQNRWYYDSSDGVCKQMQFKGRRGNGNRFLTRQDCEASCRE-------------------SQNICTLPKVRGPCNSQIDSFWFDEDKNSCFKFTWGGCQGNGNRFESMEFCEAKCKDTGSGAPSKEKANVDICNLPIDSGPCT---DRIPNWYFNTKNGKCSAFIYSGCEGN---ANRFITQEQCDRNCGIFKGKNV----------CGSQMDIGPCVGRFKKWYFDLHTRTCHEFTFGGCEGNGNRFSSLGECETVCLVQDELPIQGNNTEVSRSEICMLQLDEGSCDDQLKRWYFNEERNICAPFLYTGCAGNRNRFKSYDICMGFC 1705 HSP 4 Score: 80.8777 bits (198), Expect = 9.671e-16 Identity = 43/132 (32.58%), Postives = 68/132 (51.52%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC----------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNS 356
C M GPC +++++ T C F FGGC+GNGN F SL ECET C + + + + C+ L +GSC ++++F+ + + C+ F+Y+GC GN N F S C C P+ ++
Sbjct: 1584 CGSQMDIGPCVGRFKKWYFDLHTRTCHEFTFGGCEGNGNRFSSLGECETVCLVQDELPIQGNNTEVSRSEICMLQLDEGSCDDQLKRWYFNEERNICAPFLYTGCAGNRNRFKSYDICMGFCGPKNPPSKDN 1715
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000011000 (supercontig:LSalAtl2s:LSalAtl2s73:722677:726549:1 gene:EMLSAG00000011000 transcript:EMLSAT00000011000 description:"maker-LSalAtl2s73-augustus-gene-7.20") HSP 1 Score: 142.51 bits (358), Expect = 9.162e-37 Identity = 99/349 (28.37%), Postives = 155/349 (44.41%), Query Frame = 0
Query: 208 DSTTTTLPSTTPA-STLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP--EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
DS+TT S S S T + +PC LA GPC ++RF YN ++ C+ F++GGC GN NNF+++E C+ C P +D PC P +G C G+ ++++F+ C F++ GC N NNF SL +C C D + ++ + C PI G+C+ YGF+ +C+ + YSGC G++N F S E CK C S S+ +++ K++G E ++ + L C + GSC G R+Y++ + C +F + GC GN NN++S + C C
Sbjct: 140 DSSTTPFESVEDCNSQCISPTYAVNKNPCTLAPDPGPCRGSMVRFHYNHESRLCDKFIYGGCVGNENNFRTVESCQKKCDVPLPISLEDPPCSIPPYRGPCRGNIQRWYFNNVKKICETFIFGGCLSNKNNFLSLEDCNDKC-DQRSGSPSTANK------------CFLPIDVGNCKASLPRYGFDVGSKKCIVFNYSGCEGNNNRFESLEDCKRNC-------------------SGSFLDTEGDNQDDD--------EEEKKVGEEITSKT---------------------LVDCTSDADSGSCKGMFMRYYFD--GKQCSTFIYGGCLGNNNNYKSAEKCKEAC 425 HSP 2 Score: 105.916 bits (263), Expect = 1.719e-24 Identity = 91/346 (26.30%), Postives = 152/346 (43.93%), Query Frame = 0
Query: 301 LKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIA-----PRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC-------------HDAEVRADGSILTNASI--CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNR 624
L +C G+ +++F + TC S C + F+S+ +C S C + PT + V K + P G C+ + +N C +IY GC G++N F + E+C+ +C++ + P C I PC + W ++ +++C + GC + K N F + +C C RS S P A C L + G+C SLPR+ ++ + C F +SGC GN N F+S + C C D E + G +T+ ++ C G C+ R++++ + C F YGGC G+ NN+ + +C+ C R
Sbjct: 111 LPKNCKGTNSQWYFAEEEGTCVIRNLSECDSSTTPFESVEDCNSQC---ISPT--------YAVNKNPCTLAPDP---GPCRGSMVRFHYNHESRLCDKFIYGGCVGNENNFRTVESCQKKCDVPLPISLEDPPCSIPPYRGPCRGN---IQRWYFNNVKKICETFIFGGCLSNK---NNFLSLEDCNDKC-DQRSGS----PSTA-NKCFLPIDVGNCKASLPRYGFDVGSKKCIVFNYSGCEGNNNRFESLEDCKRNCSGSFLDTEGDNQDDDEEEKKVGEEITSKTLVDCTSDADSGSCKGMFMRYYFDG--KQCSTFIYGGCLGNNNNYKSAEKCKEACSGR 428 HSP 3 Score: 96.2857 bits (238), Expect = 2.636e-21 Identity = 91/293 (31.06%), Postives = 130/293 (44.37%), Query Frame = 0
Query: 378 SGDCQLEQGVYG------FNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCL----PSPSYTSV--WVYSEEQRMC--VVTVGCSNTKRIGNE--FSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC----LNREVDAFIQFLAEESETFHEESTSSGVV 650
+G C+L + G F + E CV S C S F S E C ++C I+P + PC P P S+ + Y+ E R+C + GC +GNE F T C++ C SLE P C + P RG C G++ R+Y+ + + C +F F GC N NNF S + C C + GS T A+ C P G C+ PR+ ++ + C F Y GC+G+ N F + C+ C L+ E D Q EE + EE TS +V
Sbjct: 106 NGKCKLPKNCKGTNSQWYFAEEEGTCVIRNLSECDSSTTPFESVEDCNSQC---ISPTYAVNKN--PCTLAPDPGPCRGSMVRFHYNHESRLCDKFIYGGC-----VGNENNFRTVESCQKKCDVPLPISLEDPP------CSIPPYRGPCRGNIQRWYFNNVKKICETFIFGGCLSNKNNFLSLEDCNDKCD----QRSGSPST-ANKCFLPIDVGNCKASLPRYGFDVGSKKCIVFNYSGCEGNNNRFESLEDCKRNCSGSFLDTEGDN--QDDDEEEKKVGEEITSKTLV 375
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000009168 (supercontig:LSalAtl2s:LSalAtl2s579:234046:258823:-1 gene:EMLSAG00000009168 transcript:EMLSAT00000009168 description:"maker-LSalAtl2s579-augustus-gene-2.12") HSP 1 Score: 145.591 bits (366), Expect = 4.357e-36 Identity = 127/443 (28.67%), Postives = 197/443 (44.47%), Query Frame = 0
Query: 232 GSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC---------SEHKPE-KDDPCISPLLKGS--CSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNS-----LGRHIFRVP----KRSLSDCLKPITSG----DCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLS-----------HEACKNRCEIQIAPRCRIEH---TFEPCLPSP----SYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRP---ERG-SCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
G+ C GPC + LI ++YN T++CE+F FGGC+GN NNFKSL +C+ C SE P K + C S + G C+G +KF F++ + TC +VY GCG N +D+L EC S+C PT + G+ ++P + + D L P+ G + +E+ Y ++ N CV + +S E+ +++ I I P+ + E P P +Y W Y +++ CV + GC TK N F++ ++CE +C S S + + C L E G C + + +C + GC N F S Q C + C E +GS +C P G CR +P++ +N + CE+F Y GC+G+ N F + +C TC E
Sbjct: 114 GNKCSQPKETGPCRMILIAYYYNANTDRCESFYFGGCRGNDNNFKSLADCQKECHSSSYNPSSSELNPSVKSEHCKSDPIAGQMFCAGYFDKFTFNYDTLTCEPYVYGGCGATANLYDTLQECMSSCVHGSAPTGGAPMESRQGKEAIKLPHWKEEEDICD-LPPVHRGPITCEAYIEKWTYD-KKSGNSCVHGQVGASVTEKTIPVSFFRSAPTGGAPMESRQDKDAI-IFPQWKEEEDICDLPPVHKGPIMCAAYIEKWTYDKKRGKCVQFIHGGCFGTK---NNFASKADCENSCPKTPSRSTKPPQFIRPDFCSLPAVDNEGGIQCLALSYGYTFNGAKGSCEQIVYGGCGATANYFSSLQKCKSAC---EGEGNGS-QKFIDVCTLPTEAGKCRARKPKYTFNQETQRCEKFFYTGCEGNGNGFDSVEECFDTCGGAE 546 HSP 2 Score: 134.42 bits (337), Expect = 1.452e-32 Identity = 117/436 (26.83%), Postives = 188/436 (43.12%), Query Frame = 0
Query: 250 RFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKD----------DPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMN-SLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSV--WVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSD------RSHSLEV-EPMF--------ALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCH---DAEVRA----------DGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHE 642
R+ Y+ ++CE + G CK N F + +ECE C + P D C ++ G+C +++ + C F Y+GC GN NNF + +C+ C + + + + + + C +P SG C+ + Y F++ E C ++Y GC G+ N F + + C CE+ I + + + P P + + W Y ++ C + GC+ GN+F + ECE TC SD R + P+ ++ CLL G C +L +FY++ + C F + GC+GNGNNF S C C VR+ +G + + C P G CR + R +N + CE+F YGGC G+ NNF + +C TCL V + + HE
Sbjct: 930 RYRYDLSKDRCEPYTTGLCKPGPNRFSTQQECELKCVQSPPRIDLTKDNIPGLLPKCNLNVIPGNCRSRIKRYFYSPAHGGCRFFYYTGCQGNENNFLTAEKCEKACVPSTEVRSSFQVATEPNEIVEEIKNPCDQPKNSGLCKAMKPSYYFDKDEGVCKFFLYGGCMGNANRFFTLKECSLTCEVTIEKQSEGNYCKKAPEPGPCFAIIRKWYYDPKENDCKLFFYGGCAGN---GNKFDSKQECELTCSSDFDIPQGRIKDKNIPNPVIDDSILSTGMVDPCLLDKTIGDCRAALNKFYFDKESKECKLFKYGGCNGNGNNFPSEDICKQVCSRHIQVPVRSNEPPKTVTTEEGDEVAFSPNCFSPVDVGVCRGSKIRHHFNAASGLCEKFIYGGCLGNENNFLSEEECYDTCLPNLVHSQTDLKHKVDAKIHE 1362 HSP 3 Score: 126.716 bits (317), Expect = 4.591e-30 Identity = 133/517 (25.73%), Postives = 208/517 (40.23%), Query Frame = 0
Query: 191 LVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNG---NNFKSLEECETTCSEHKPEKDDP------------CISPLLKGSCSGS-----GEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC----------EIQIAPRCRIEHTFEPCLPSPSYTSV-------WVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC-----------HDAEVR---ADGSILTNASI--CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVA 652
+V ++ +SL+ + + ++ +PAS + S C L A GPC + +FF+N KC F +GG + N FKS ECE C P K+ P C++PL G SG ++ +D C + C N F + EC+ C P ++ +I + L C + G+C+ Y ++ C + Y+GC G++N FL+ E C+ C ++ P +E PC P + + + + +++ +C + GC N F T EC TC + +E C PE G C + ++YY+P + C F + GC GNGN F S Q C TC D + D SIL+ + C K G CR +F+++ + C+ F+YGGC G+ NNFP+ C+ C IQ +E +T G VA
Sbjct: 794 VVQMRIPKSLQ-NMMKNAPLDSSFQKHSPASVGAQGKSH---DDCLLPPAVGPCKGFRDKFFFNIDNRKCTAFKYGGFDEDAQGFNVFKSKGECEKVCERLLPSKEIPKPVLQQQQVDSECVTPLHHGITSGQLCLPLXRRYRYDLSKDRCEPYTTGLCKPGPNRFSTQQECELKCV-QSPPRIDLTKDNIPGL----LPKCNLNVIPGNCRSRIKRYFYSPAHGGCRFFYYTGCQGNENNFLTAEKCEKACVPSTEVRSSFQVATEPNEIVEEIKNPC-DQPKNSGLCKAMKPSYYFDKDEGVCKFFLYGGCMGN---ANRFFTLKECSLTC------EVTIEKQSEGNYCKKAPEPGPCFAIIRKWYYDPKENDCKLFFYGGCAGNGNKFDSKQECELTCSSDFDIPQGRIKDKNIPNPVIDDSILSTGMVDPCLLDKTIGDCRAALNKFYFDKESKECKLFKYGGCNGNGNNFPSEDICKQVCSRH-----IQVPVRSNEPPKTVTTEEGDEVA 1286 HSP 4 Score: 107.071 bits (266), Expect = 6.665e-24 Identity = 133/564 (23.58%), Postives = 205/564 (36.35%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNE---NNFRYKKACERMC---MNGNTTEEEILRQEPRRS-----------LKNLTLDFDQGFEDWDNYDWQIISYNSDLAR-GLNLYSPDPGS--RSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC--SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD----------CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
CLL P G C + ++FFN + C F + G + N F+ K CE++C + + +L+Q+ S L L + + +D + Y + L + G N +S + P R + P L+ ++ I ++ R P F + N +T + S+ +T + E +PC G C ++++ C+ F++GGC GN N F +L+EC TC + K + + C G C K+++D + + C F Y GC GNGN FDS EC+ TC D + +P + D CL T GDC+ + F++ +C + Y GC G+ N F S + CK C I V V S E V T G+E + S C P+ + G C GS R ++ C F + GC GN NNF S + C TC
Sbjct: 834 CLLPPAVGPCKGFRDKFFFNIDNRKCTAFKYGGFDEDAQGFNVFKSKGECEKVCERLLPSKEIPKPVLQQQQVDSECVTPLHHGITSGQLCLPLXRRYR-YDLSKDRCEPYTTGLCKPGPNRFSTQQECELKCVQSPPRIDLTKDNIPGLLPKCNLN---VIPGNCRSRIKRYFYSPAHGGCRFFYYTGCQGNENNFLTAEKCEKACVPSTEVRSSFQVATEPNEIVEEIKNPCDQPKNSGLCKAMKPSYYFDKDEGVCKFFLYGGCMGNANRFFTLKECSLTCEVTIEKQSEGNYCKKAPEPGPCFAIIRKWYYDPKENDCKLFFYGGCAGNGNKFDSKQECELTCSSDFDIPQGRIKDK--NIPNPVIDDSILSTGMVDPCLLDKTIGDCRAALNKFYFDKESKECKLFKYGGCNGNGNNFPSEDICKQVCSRHI--------------------QVPVRSNEPPKTVTT-------EEGDEVAFSPNC-------------FSPV----------DVGVCRGSKIRHHFNAASGLCEKFIYGGCLGNENNFLSEEECYDTC 1341 HSP 5 Score: 94.3597 bits (233), Expect = 4.784e-20 Identity = 100/441 (22.68%), Postives = 156/441 (35.37%), Query Frame = 0
Query: 244 CDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH----------------KPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC--------KDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-------------------------CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVV--TVGCSNT-KRIGNEFSTSSECERTCLSDRSHSLEVE-PMFALEG--CLLRPERGS---CHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSI-LTNASICEQPKLKG----PCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C Y +F + + T CE +++GGC N + +L+EC ++C + C P G C ++++ + C F + GC GN NNF SLA+CQ C ++P++ S H P C + FN C PY+Y GCG + NL+ + + C + C AP C + + +Y W Y ++ V VG S T K I F S+ + R + P + E C L P C + ++ Y+ C F GC G NNF S C +C R+ C P + C + +N + +CE+ YGGC + N F + +C++ C
Sbjct: 49 CAGYFPKFTFKYDTMSCEPYIYGGCGATANLYDTLQECMSSCVHGMRFCALLQILLCFNVVTARPGNKCSQPKETGPCRMILIAYYYNANTDRCESFYFGGCRGNDNNFKSLADCQKECHSSSYNPSSSELNPSVKS--EHCKSDPIAGQMFCAGYFDK---------FTFNYDTLTCEPYVYGGCGATANLYDTLQECMSSCVHGSAPTGGAPMESRQGKEAIKLPHWKEEEDICDLPPVHRGPITCEAYIEKWTYDKKSGNSCVHGQVGASVTEKTIPVSFFRSAPTGGAPMESRQDKDAIIFPQWKEEEDICDLPPVHKGPIMCAAYIEKWTYDKKRGKCVQFIHGGCFGTKNNFASKADCENSCPKTPSRSTKPPQFIRPDFCSLPAVDNEGGIQCLALSYGYTFNGAKGSCEQIVYGGCGATANYFSSLQKCKSAC 478 HSP 6 Score: 73.9442 bits (180), Expect = 1.020e-13 Identity = 42/132 (31.82%), Postives = 64/132 (48.48%), Query Frame = 0
Query: 501 EGCLLRPERGS--CHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC-HDAEVRADGSILTNASI--------CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
E C P G C G P+F ++ +C + + GC N + + Q C+++C H A IL ++ C QPK GPCR ++YN + + CE F +GGC+G+ NNF + A C+ C
Sbjct: 36 EHCKSEPVTGEIFCAGYFPKFTFKYDTMSCEPYIYGGCGATANLYDTLQECMSSCVHGMRFCALLQILLCFNVVTARPGNKCSQPKETGPCRMILIAYYYNANTDRCESFYFGGCRGNDNNFKSLADCQKEC 167 HSP 7 Score: 65.855 bits (159), Expect = 2.987e-11 Identity = 51/175 (29.14%), Postives = 74/175 (42.29%), Query Frame = 0
Query: 463 GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRP--------ERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASI----CEQPKLKGP--CRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
GC T N + T EC +C+ ++ + RP E G C L +YY + C SF F GC GN NNF+S C CH + S L N S+ C+ + G C +F +N TCE + YGGC + N + T +C ++C++
Sbjct: 72 GCGAT---ANLYDTLQECMSSCVHGMRFCALLQILLCFNVVTARPGNKCSQPKETGPCRMILIAYYYNANTDRCESFYFGGCRGNDNNFKSLADCQKECHSSSYNPSSSEL-NPSVKSEHCKSDPIAGQMFCAGYFDKFTFNYDTLTCEPYVYGGCGATANLYDTLQECMSSCVH 242 HSP 8 Score: 53.9138 bits (128), Expect = 1.449e-7 Identity = 25/62 (40.32%), Postives = 37/62 (59.68%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPC 296
C L G C ++ +N +T++CE F + GC+GNGN F S+EEC TC +PE++ C
Sbjct: 492 CTLPTEAGKCRARKPKYTFNQETQRCEKFFYTGCEGNGNGFDSVEECFDTCGGAEPEQNAFC 553
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000012691 (supercontig:LSalAtl2s:LSalAtl2s961:84758:119265:1 gene:EMLSAG00000012691 transcript:EMLSAT00000012691 description:"maker-LSalAtl2s961-snap-gene-1.23") HSP 1 Score: 127.872 bits (320), Expect = 1.752e-30 Identity = 98/335 (29.25%), Postives = 143/335 (42.69%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP-------EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKP--------ITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSD-RSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
C L G C + R++YN ++C+ FV+GGCKGNGNNF + +C C E + D C +G C G+ +F+FD ++ C F Y GCGGN NNF++ EC + C + VP + LKP ++ G+C + F++ + C +I+ GC G+DN F + E C C P+ V + + R E S SS L D + +S V C L + G C R+YY+P +TC F +SGC GN NNF+S + C+ TC
Sbjct: 1046 CTLPKNSGGCSNKIFRYYYNAIEQRCKLFVYGGCKGNGNNFLTERQCLLRCGEEGSLSQTEITTEVDTCSQKRDEGICPGNVPRFYFDKKAGRCLLFSYGGCGGNSNNFENQDECIARCGGPKGTII---------VPSSLTNGSLKPRKLICSLQLSRGNCXATLRRFYFDEKQG-CKLFIFGGCQGNDNNFETMEDCIRNCG------------------GPNEHGVSEFINQLR----------------EQSLSSSSTNLALPDLKENSANV--------CSLPKDFGHCMTVTSRYYYDPKTDTCKKFRYSGCGGNTNNFRSGERCIETC 1328 HSP 2 Score: 101.293 bits (251), Expect = 3.415e-22 Identity = 87/316 (27.53%), Postives = 133/316 (42.09%), Query Frame = 0
Query: 372 CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPRCRIEHTFEPCLPS------PSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSL--------EVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC------------HDAEVRADGSILTN----------ASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN-REVDAFIQFLAEESETFHEESTSS 647
C P SG C + Y +N E +C ++Y GC G+ N FL+ C RC E + I + C P + + ++ C++ GC N F EC C + + ++P + C L+ RG+C +L RFY++ + C F F GC GN NNF++ + C+ C + ++ S TN A++C PK G C R++Y+P +TC++FRY GC G+ NNF + +C TC E D I L T + +TSS
Sbjct: 1046 CTLPKNSGGCSNKIFRYYYNAIEQRCKLFVYGGCKGNGNNFLTERQCLLRCGEEGSLSQTEITTEVDTCSQKRDEGICPGNVPRFYFDKKAGRCLLFSYGGCGGN---SNNFENQDECIARCGGPKGTIIVPSSLTNGSLKPRKLI--CSLQLSRGNCXATLRRFYFDE-KQGCKLFIFGGCQGNDNNFETMEDCIRNCGGPNEHGVSEFINQLREQSLSSSSTNLALPDLKENSANVCSLPKDFGHCMTVTSRYYYDPKTDTCKKFRYSGCGGNTNNFRSGERCIETCGGLLEKDNRISALTLSPFTIIKATTSS 1355 HSP 3 Score: 62.3882 bits (150), Expect = 3.389e-10 Identity = 84/338 (24.85%), Postives = 124/338 (36.69%), Query Frame = 0
Query: 21 FCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMC-MNGNTTEEEILRQEPRRSLKNLTLDFDQG---------FEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGP----LRPQGFSKSH---PKLMHPIQVDRNEAITITLSL--------KLLRIESWPGRRNNSFIVMNDLV----------VLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPC----ISP--LLKGSCSGSGEKFHFDFQ 317
C L G C RY++N C+ F + GC N NNF ++ C C G+ ++ EI + S K D+G + D + SY N + D C GP + P + KL+ +Q+ R KL G NN F M D + V + LR ++++ ST LP E + + C L G C R++Y+ KT+ C+ F + GC GN NNF+S E C TC EKD+ +SP ++K + S + H F
Sbjct: 1045 ICTLPKNSGGCSNKIFRYYYNAIEQRCKLFVYGGCKGNGNNFLTERQCLLRCGEEGSLSQTEITTEVDTCSQKR-----DEGICPGNVPRFYFDKKAGRCLLFSYGGCGGNSNNFENQDECIARCGGPKGTIIVPSSLTNGSLKPRKLICSLQLSRGNCXATLRRFYFDEKQGCKLFIFGGCQGNDNN-FETMEDCIRNCGGPNEHGVSEFINQLREQSLSSSSTNLALPDL----------KENSANVCSLPKDFGHCMTVTSRYYYDPKTDTCKKFRYSGCGGNTNNFRSGERCIETCGGLL-EKDNRISALTLSPFTIIKATTSSTSNTLHKSFH 1365
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000000151 (supercontig:LSalAtl2s:LSalAtl2s1023:177971:188369:1 gene:EMLSAG00000000151 transcript:EMLSAT00000000151 description:"maker-LSalAtl2s1023-augustus-gene-1.11") HSP 1 Score: 122.865 bits (307), Expect = 3.918e-29 Identity = 118/488 (24.18%), Postives = 193/488 (39.55%), Query Frame = 0
Query: 17 VIPEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPG--RRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCS---EHKPEKDDPCISPLLKGSCSGSGE-KFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHI-----------FRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTC 484
++ C L + G C +Y+FN+ +CE+F++ GC N+N F C +C + +E+ P S K T + ED+D Y+ I N + N+ D C P+ +K P+ + +Q + W G + N+FI + + + S+I ++ TLP P+ C +FFYN K KC + CK N N + +++ +HK + C P G+C+ + KF+FD + + C KF YSGC GNGN FDS+ C +CK + +GR + + + C +G C+ Y N +C ++Y GC G+ N F S ++C+ C + C +E CL + Y + Q+ C+ + GC N FST +C++TC
Sbjct: 24 ILKSTCTLNKDPGTCAKNITQYYFNKDLKSCESFTWGGCEGNKNRFDTVLECNDVCQ---PSSKELSDNHPELSQKKKTQESSD--EDYDYYNLNFIQENKNEEVARNI--SDRDGSICGLPVEIGNCTKHSPRFFYNMQSGSCQFFI------------WKGCVKNENNFISIKKCLDKCHGVVKKESSITQVTSQCTLPPKNPS-----------------------CHELRSKFFYNPKKNKCVKRLH--CKDNPNGYXXIKDMLKAMQAKFKHKRKSIRRCFEPRDPGTCTKNFRLKFYFDSKQNKCLKFYYSGCNGNGNRFDSITNCVLSCKLKEQFSFQGVGRAVKYLNYLLDPKLLIIATPTYDTCSLQSETGPCEESVVQYFHNAKTGKCETFVYGGCYGNGNNFDSMKSCEEHCIQNSYNDACLVEKRRGYCLKK---KVAYHYDKNQQKCLSFIYGGCGGN---ANRFSTMDQCQKTC 461 HSP 2 Score: 121.324 bits (303), Expect = 1.336e-28 Identity = 107/461 (23.21%), Postives = 183/461 (39.70%), Query Frame = 0
Query: 230 LAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC-------SEHKPEKDDP--------------------------------------CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIA-PRCRIEHTFEPCLP---SPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLE------------VEPMFAL------EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
+ S C L G C + ++++N + CE+F +GGC+GN N F ++ EC C S++ PE C P+ G+C+ +F ++ QS +C F++ GC N NNF S+ +C C + + I +V S C P + C + + +N +N+CV ++ C + N + + + + R I FEP P + ++ + + +Q C+ GC+ GN F + + C +C S + ++P + + C L+ E G C S+ ++++ C +F + GC+GNGNNF S + C C C K +G C + + + Y+ + + C F YGGC G+ N F T QC+ TC
Sbjct: 24 ILKSTCTLNKDPGTCAKNITQYYFNKDLKSCESFTWGGCEGNKNRFDTVLECNDVCQPSSKELSDNHPELSQKKKTQESSDEDYDYYNLNFIQENKNEEVARNISDRDGSICGLPVEIGNCTKHSPRFFYNMQSGSCQFFIWKGCVKNENNFISIKKCLDKCHGVVKKESS-----ITQV----TSQCTLPPKNPSCHELRSKFFYNPKKNKCVKRLH--CKDNPNGYXXIKDMLKAMQAKFKHKRKSIRRCFEPRDPGTCTKNFRLKFYFDSKQNKCLKFYYSGCNGN---GNRFDSITNCVLSCKLKEQFSFQGVGRAVKYLNYLLDPKLLIIATPTYDTCSLQSETGPCEESVVQYFHNAKTGKCETFVYGGCYGNGNNFDSMKSCEEHCIQNSYN---------DACLVEKRRGYCLKKKVAYHYDKNQQKCLSFIYGGCGGNANRFSTMDQCQKTC 461 HSP 3 Score: 110.153 bits (274), Expect = 4.106e-25 Identity = 86/376 (22.87%), Postives = 150/376 (39.89%), Query Frame = 0
Query: 303 GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCK-------------------------DHMDPTMNSLGRHIFRVPKRSLSD-----CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPS------YTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLP-RFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAE---VRADGSILTNASICEQPKL----------------KGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
G+C+ + +++F+ +C F + GC GN N FD++ EC C+ D+ +N + + R++SD C P+ G+C + +N C +I+ GC ++N F+S + C ++C + I C P S + Y+ ++ CV + C + N + + + + H + ++ C + G+C + +FY++ C F +SGC+GNGN F S +C+ +C E + G + + PKL GPC ++F+N CE F YGGC G+ NNF + CE C+
Sbjct: 36 GTCAKNITQYYFNKDLKSCESFTWGGCEGNKNRFDTVLECNDVCQPSSKELSDNHPELSQKKKTQESSDEDYDYYNLNFIQENKNEEVARNISDRDGSICGLPVEIGNCTKHSPRFFYNMQSGSCQFFIWKGCVKNENNFISIKKCLDKCHGVVKKESSITQVTSQCTLPPKNPSCHELRSKFFYNPKKNKCVKRLHCKDNP---NGYXXIKDMLKAMQAKFKHKRK-----SIRRCFEPRDPGTCTKNFRLKFYFDSKQNKCLKFYYSGCNGNGNRFDSITNCVLSCKLKEQFSFQGVGRAVKYLNYLLDPKLLIIATPTYDTCSLQSETGPCEESVVQYFHNAKTGKCETFVYGGCYGNGNNFDSMKSCEEHCI 403 HSP 4 Score: 103.219 bits (256), Expect = 7.538e-23 Identity = 94/379 (24.80%), Postives = 158/379 (41.69%), Query Frame = 0
Query: 223 LSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCIS--------PLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLE-QGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEI------QIAPRC--RIEHTFEPCL---PSPSYTSVWVYSE----EQRMC-------------VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSI 564
++ + S+ GS C L + G C + RFFYN ++ C+ F++ GC N NNF S+++C C H K + I+ P SC KF ++ + + C K ++ C N N + + + M + F+ ++S+ C +P G C + + F+ +N+C+ + YSGC G+ N F S C C++ Q R + + +P L +P+Y + + SE E+ + V GC GN F + CE C+ + + + CL+ RG C ++Y+ + C SF + GC GN N F + C TC + + SI
Sbjct: 123 VARNISDRDGSICGLPVEIGNCTKHSPRFFYNMQSGSCQFFIWKGCVKNENNFISIKKCLDKC--HGVVKKESSITQVTSQCTLPPKNPSCHELRSKFFYNPKKNKCVKRLH--CKDNPNGYXXIKD------------MLKAMQAKFKHKRKSIRRCFEPRDPGTCTKNFRLKFYFDSKQNKCLKFYYSGCNGNGNRFDSITNCVLSCKLKEQFSFQGVGRAVKYLNYLLDPKLLIIATPTYDTCSLQSETGPCEESVVQYFHNAKTGKCETFVYGGCYGN---GNNFDSMKSCEEHCIQNSYN----------DACLVEKRRGYCLKKKVAYHYDKNQQKCLSFIYGGCGGNANRFSTMDQCQKTCTPIKALSSASI 472 HSP 5 Score: 95.9005 bits (237), Expect = 1.145e-20 Identity = 47/105 (44.76%), Postives = 64/105 (60.95%), Query Frame = 0
Query: 242 GPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
GPC ++ RF+YN + KCE+F FGGC NGN+FK LE C C+ ++ K CI P G C +KF+++ + C F+YSGCGGN N FDS +C+ C
Sbjct: 732 GPCQNFVKRFYYNANSTKCESFYFGGCFDNGNHFKELESCINHCAINEYPKS--CIEPQDVGECKEKIKKFYYEKDTGACHSFIYSGCGGNTNRFDSQRDCERIC 834 HSP 6 Score: 70.8626 bits (172), Expect = 7.423e-13 Identity = 45/143 (31.47%), Postives = 62/143 (43.36%), Query Frame = 0
Query: 505 LRPE-RGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTS 646
LRP+ G C + RFYY C SF F GC NGN+F+ + C+ C +I C +P+ G C+ +F+Y C F Y GC G+ N F ++ CE C V+ EE FH + S
Sbjct: 726 LRPKFIGPCQNFVKRFYYNANSTKCESFYFGGCFDNGNHFKELESCINHC---------AINEYPKSCIEPQDVGECKEKIKKFYYEKDTGACHSFIYSGCGGNTNRFDSQRDCERICSPYFVEK-----EEERVEFHNQKES 854 HSP 7 Score: 67.3958 bits (163), Expect = 8.052e-12 Identity = 39/133 (29.32%), Postives = 62/133 (46.62%), Query Frame = 0
Query: 290 PEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
PE+++ C+ P G C ++F+++ S C F + GC NGN+F L C + C I PK C++P G+C+ + + + + C +IYSGCGG+ N F S C+ C
Sbjct: 719 PEQEEICLRPKFIGPCQNFVKRFYYNANSTKCESFYFGGCFDNGNHFKELESCINHCA-------------INEYPK----SCIEPQDVGECKEKIKKFYYEKDTGACHSFIYSGCGGNTNRFDSQRDCERIC 834
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000008877 (supercontig:LSalAtl2s:LSalAtl2s554:325027:327698:-1 gene:EMLSAG00000008877 transcript:EMLSAT00000008877 description:"snap_masked-LSalAtl2s554-processed-gene-3.24") HSP 1 Score: 110.538 bits (275), Expect = 2.419e-25 Identity = 77/249 (30.92%), Postives = 107/249 (42.97%), Query Frame = 0
Query: 242 GPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-----CRIEHTFEPCLPS-PSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTC 484
G C + I++FY+ C F +GGC N+F + EEC C P C + GSC + +F++D++ +C FVY GC GNGN F S C C ++ D MN VP C KP+ G C + + ++ E +C + YSGCGG++N FLS C N C R CR + P + + W Y R C+ S FST EC C
Sbjct: 222 GSCSDFQIKWFYDATWGGCNKFWYGGCDPGENHFDTEEECIGICV--NPRGSQVCYLDMNTGSCDATYNEFYYDYKRGSCVPFVYGGCLGNGNRFTSKKSCNELCGNNND--MN--------VPV-----CSKPMAEGMCLGDFNRWYYDYEEGKCKNFSYSGCGGNNNRFLSEVECLNTCRKDTKERLSKTVCRSSYQITPSIKDGGTIFPAWTYDSLVRRCIPLYASSAEMEQDILFSTWEECSNIC 453 HSP 2 Score: 98.9821 bits (245), Expect = 1.289e-21 Identity = 62/197 (31.47%), Postives = 90/197 (45.69%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC-KDHMDPTMNSLGRHIFRV-PKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVP-YIYSGCGGSDNLFLSHEACKNRCEIQIAP 428
C L M G CD F+Y++K C FV+GGC GNGN F S + C C + C P+ +G C G ++++D++ C F YSGCGGN N F S EC +TC KD + ++ R +++ P + P + ++ +C+P Y S D LF + E C N C P
Sbjct: 274 CYLDMNTGSCDATYNEFYYDYKRGSCVPFVYGGCLGNGNRFTSKKSCNELCGNNNDMNVPVCSKPMAEGMCLGDFNRWYYDYEEGKCKNFSYSGCGGNNNRFLSEVECLNTCRKDTKERLSKTVCRSSYQITPSIKDGGTIFP-----------AWTYDSLVRRCIPLYASSAEMEQDILFSTWEECSNICPTTYPP 459 HSP 3 Score: 77.0258 bits (188), Expect = 9.139e-15 Identity = 62/237 (26.16%), Postives = 94/237 (39.66%), Query Frame = 0
Query: 389 GFNQTENQCVPYIYSGC--GGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
G +++ ++C Y C G + L L E C + ++ +C + C + W Y C GC + N F T EC C++ R + C L GSC + FYY+ +C F + GC GNGN F S + C C + N +C +P +G C D R++Y+ C+ F Y GC G+ N F + +C TC
Sbjct: 172 GASESCDECQNTKYGCCFDGNTTALGLHFEGCGSNLLEELPKKCSSKSRIGSC---SDFQIKWFYDATWGGCNKFWYGGCDPGE---NHFDTEEECIGICVNPRGSQV----------CYLDMNTGSCDATYNEFYYDYKRGSCVPFVYGGCLGNGNRFTSKKSCNELCGNNND-------MNVPVCSKPMAEGMCLGDFNRWYYDYEEGKCKNFSYSGCGGNNNRFLSEVECLNTC 385 HSP 4 Score: 52.373 bits (124), Expect = 3.720e-7 Identity = 33/121 (27.27%), Postives = 51/121 (42.15%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
C + GSC +++Y+ C+ F + GC N+F + + C+ C V GS +C G C F+Y+ +C F YGGC G+ N F ++ C C N
Sbjct: 215 CSSKSRIGSCSDFQIKWFYDATWGGCNKFWYGGCDPGENHFDTEEECIGIC----VNPRGS-----QVCYLDMNTGSCDATYNEFYYDYKRGSCVPFVYGGCLGNGNRFTSKKSCNELCGN 326
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000003009 (supercontig:LSalAtl2s:LSalAtl2s170:853996:854817:1 gene:EMLSAG00000003009 transcript:EMLSAT00000003009 description:"augustus_masked-LSalAtl2s170-processed-gene-8.0") HSP 1 Score: 93.2041 bits (230), Expect = 2.652e-21 Identity = 43/123 (34.96%), Postives = 67/123 (54.47%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C ++P+ G C LP+FYY+ C SF + GC GN N F + ++CL+ C E + ++C+ GPCR R+F++ + C EF YGGC+G+ NNF ++ CE C+ +
Sbjct: 137 CHMKPDPGPCRSLLPQFYYDFETGRCKSFYYGGCQGNANRFDNEENCLSICSSEEKKKGRE--NKGNVCKLKADPGPCRALFQRYFFDSEKKVCVEFGYGGCQGNENNFVKKSDCEDVCVKND 257 HSP 2 Score: 81.6481 bits (200), Expect = 2.619e-17 Identity = 41/129 (31.78%), Postives = 68/129 (52.71%), Query Frame = 0
Query: 223 LSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPE-----KDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
++ S+ S C + GPC L +F+Y+++T +C++F +GGC+GN N F + E C + CS + + K + C G C +++ FD + C +F Y GC GN NNF ++C+ C
Sbjct: 125 ITDMKSKEKPSQCHMKPDPGPCRSLLPQFYYDFETGRCKSFYYGGCQGNANRFDNEENCLSICSSEEKKKGRENKGNVCKLKADPGPCRALFQRYFFDSEKKVCVEFGYGGCQGNENNFVKKSDCEDVC 253 HSP 3 Score: 56.9954 bits (136), Expect = 3.937e-9 Identity = 36/120 (30.00%), Postives = 55/120 (45.83%), Query Frame = 0
Query: 303 GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
G C +F++DF++ C F Y GC GN N FD+ C S C +S + R K ++ C G C+ Y F+ + CV + Y GC G++N F+ C++ C
Sbjct: 144 GPCRSLLPQFYYDFETGRCKSFYYGGCQGNANRFDNEENCLSIC--------SSEEKKKGRENKGNV--CKLKADPGPCRALFQRYFFDSEKKVCVEFGYGGCQGNENNFVKKSDCEDVC 253 HSP 4 Score: 52.373 bits (124), Expect = 1.305e-7 Identity = 23/62 (37.10%), Postives = 35/62 (56.45%), Query Frame = 0
Query: 229 ELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP 290
E G+ C+L GPC R+F++ + + C F +GGC+GN NNF +CE C ++ P
Sbjct: 197 ENKGNVCKLKADPGPCRALFQRYFFDSEKKVCVEFGYGGCQGNENNFVKKSDCEDVCVKNDP 258
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000007269 (supercontig:LSalAtl2s:LSalAtl2s408:65936:69603:1 gene:EMLSAG00000007269 transcript:EMLSAT00000007269 description:"maker-LSalAtl2s408-snap-gene-0.18") HSP 1 Score: 97.8265 bits (242), Expect = 2.900e-21 Identity = 66/238 (27.73%), Postives = 105/238 (44.12%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLI--RFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKG--SCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCE-----IQIAPRCR----IEHTFEPCLPSPSYTSVWVYSEEQRMCV 459
C + G C Y I ++++N C F FGGC GNGN F+ CE+ C P C P + G SC+ ++FD + C +F+Y+GC GN N F + A C+ C M +L + + P C +P+ G C + ++ C ++Y GC G+ N F + E+C+N C+ ++I +CR + C S S + W + + C
Sbjct: 348 CHMTKDSGACRNYNITRQWYFNMAYGGCSRFWFGGCGGNGNRFEDRNSCESICI--NPSGTVKCYLPKVSGPPSCTSKQPHYYFDSNTKECREFIYNGCLGNANRFTTQASCEVEC-------MKALKKSGDQDP------CKQPVEPGPCNGYFPRWYYDFLRGTCSEFVYGGCKGNANQFETKESCQNSCKHKEMSLKIQKQCRKPRLVPTDGRDCHDSNSVVAKWYFDDNTHSCT 570 HSP 2 Score: 95.5153 bits (236), Expect = 1.574e-20 Identity = 76/266 (28.57%), Postives = 116/266 (43.61%), Query Frame = 0
Query: 235 CQLAMARGP--CDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSE--HKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGV---YGFNQTENQCVPYIYS-----GCG-------GSDNLFLSHEACKNRCEIQIAPRCRI---------EHTF---EPCLPSPSYTSVW------VYSEEQRMCVVTVG 463
C L GP C ++++ T++C F++ GC GN N F + CE C + K DPC P+ G C+G ++++DF TCS+FVY GC GN N F++ CQ++CK H + ++ ++ K+ L P DC V + F+ + C P+ S CG +D +F S E C + C AP I EHT +PS T +W + S + R+ + T G
Sbjct: 409 CYLPKVSGPPSCTSKQPHYYFDSNTKECREFIYNGCLGNANRFTTQASCEVECMKALKKSGDQDPCKQPVEPGPCNGYFPRWYYDFLRGTCSEFVYGGCKGNANQFETKESCQNSCK-HKEMSL--------KIQKQCRKPRLVPTDGRDCHDSNSVVAKWYFDDNTHSCTPFYLSKTDHLKCGSIMVEENANDTIFHSLEECMSICPNTYAPVISITSHVVIVKEEHTAVLQVQITSNPSPTIIWEFNGTLINSNDPRVSIDTPG 665 HSP 3 Score: 84.7297 bits (208), Expect = 3.720e-17 Identity = 52/163 (31.90%), Postives = 77/163 (47.24%), Query Frame = 0
Query: 471 GNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERG--SCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQ 631
GN F + CE C + P ++ C L G SC P +Y++ + C F ++GC GN N F + C C A ++ + C+QP GPC PR++Y+ TC EF YGGCKG+ N F T+ C+ +C ++E+ IQ
Sbjct: 387 GNRFEDRNSCESIC---------INPSGTVK-CYLPKVSGPPSCTSKQPHYYFDSNTKECREFIYNGCLGNANRFTTQASCEVECMKALKKSG-----DQDPCKQPVEPGPCNGYFPRWYYDFLRGTCSEFVYGGCKGNANQFETKESCQNSCKHKEMSLKIQ 534 HSP 4 Score: 73.1738 bits (178), Expect = 1.301e-13 Identity = 82/320 (25.62%), Postives = 128/320 (40.00%), Query Frame = 0
Query: 260 CENFVFGGCKGNGNNFK--SLEECETTCSEHKPEKDDPCISPLLKGSCSGSG--EKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSG--DCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSP--SYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERG-SCHGS---LPRFYYEPMDETCHSFTFSGC------------HGNGNNFQSFQHCLATC 553
CE FG C N + + EEC+ +KP D C G+C +++F+ CS+F + GCGGNGN F+ C+S C ++P+ C P SG C +Q Y F+ +C +IY+GC G+ N F + +C+ C + + +P P P Y W Y + C V GC K N+F T C+ +C + SL+++ L P G CH S + ++Y++ +C F S + N F S + C++ C
Sbjct: 317 CETSEFGCCPDGKNPARGENFEECQ-----NKPA--DICHMTKDSGACRNYNITRQWYFNMAYGGCSRFWFGGCGGNGNRFEDRNSCESIC---INPS--------------GTVKCYLPKVSGPPSCTSKQPHYYFDSNTKECREFIYNGCLGNANRFTTQASCEVECMKALKKSGDQDPCKQPVEPGPCNGYFPRWYYDFLRGTCSEFVYGGC---KGNANQFETKESCQNSC-KHKEMSLKIQKQCRKP--RLVPTDGRDCHDSNSVVAKWYFDDNTHSCTPFYLSKTDHLKCGSIMVEENANDTIFHSLEECMSIC 606 HSP 5 Score: 72.4034 bits (176), Expect = 2.236e-13 Identity = 52/166 (31.33%), Postives = 77/166 (46.39%), Query Frame = 0
Query: 234 PCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH----KPEKD--DPCISPLLKGSCSGSGE---KFHFDFQSHTCSKFVYS-----GCG-------GNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITS 378
PC+ + GPC+ Y R++Y++ C FV+GGCKGN N F++ E C+ +C K +K P + P C S K++FD +H+C+ F S CG N F SL EC S C + P + S+ H+ V + + ITS
Sbjct: 473 PCKQPVEPGPCNGYFPRWYYDFLRGTCSEFVYGGCKGNANQFETKESCQNSCKHKEMSLKIQKQCRKPRLVPTDGRDCHDSNSVVAKWYFDDNTHSCTPFYLSKTDHLKCGSIMVEENANDTIFHSLEECMSICPNTYAPVI-SITSHVVIVKEEHTAVLQVQITS 637
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|114152125|sp|Q868Z9.2|PPN_DROME (RecName: Full=Papilin; Flags: Precursor) HSP 1 Score: 185.267 bits (469), Expect = 2.630e-47 Identity = 129/419 (30.79%), Postives = 178/419 (42.48%), Query Frame = 0
Query: 229 ELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP------------EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRV---PKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRC---RIEHTFEPCL----PSPSYTSV--WVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
++A C++ G C Y+ ++Y+ + + C F +GGC GN N F + E C C + D C G CS K+HFD + C +F Y CGGNGN F++ +CQ C P P +++ C +P G C + +N+TE +C + Y GCGG+DN F + E C RC + I R +EH C P Y +V W Y+ + +C V GC N ++T EC+ C A C L P RG C R+Y++ CH F F+GC GN NNF S CL C E + S T S+C +P G C +FY+ C F Y GC G+ N F TR QCE C
Sbjct: 1843 QVAKDICEIPAEVGECANYVTSWYYDTQDQACRQFYYGGCGGNENRFPTEESCLARCDRKPEPTTTTPATRPQPSRQDVCDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRCLSQEPPAPTPPRAPAPTRQPDPAPTVAQCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARCSVNIDIRIGADPVEHDTSKCFLAFEPGNCYNNVTRWFYNSAEGLCDEFVYTGCGGN---ANNYATEEECQNECND------------AQTTCALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFCI-GEPVVEPSAPT-YSVCAEPPEAGECDNRTTAWFYDSENMACTAFTYTGCGGNGNRFETRDQCERQC 2244 HSP 2 Score: 167.933 bits (424), Expect = 1.243e-41 Identity = 113/400 (28.25%), Postives = 173/400 (43.25%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ--IAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCH-----DAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C L G C+ Y ++++++ C F +GGC GN N F+S EC+ TC ++ + C+ P G C+G +K++FD + C +F Y GC G N FDSL +CQ TC +L C +P+ SG C + ++ + C P+ Y GC G+ N + + AC C + RC + C + W +SE ++ CV GC K N F T CE C A + C + E G C + +YY+ D+ C F + GC GN N F + + CLA C A + +C++ G C ++ ++ I C +F YG C G+ N F T C+ CL++E
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQDYTGKH--VCLLPKSAGPCTGFTKKWYFDVDRNRCEEFQYGGCYGTNNRFDSLEQCQGTC-----------------AASENLPTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQPGVLKDRCALPKQTGDCS---EKLAKWHFSESEKRCVPFYYSGCGGNK---NNFPTLESCEDHCPR----------QVAKDICEIPAEVGECANYVTSWYYDTQDQACRQFYYGGCGGNENRFPTEESCLARCDRKPEPTTTTPATRPQPSRQDVCDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRCLSQE 1976 HSP 3 Score: 153.68 bits (387), Expect = 4.674e-37 Identity = 106/341 (31.09%), Postives = 161/341 (47.21%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLP---RFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTN 567
S C LA G C + R+FYN C+ FV+ GC GN NN+ + EEC+ C++ + C P ++G CS +++FD +S C +F ++GC GN NNF S ++C + C +G + + S C +P +G+C + ++ C + Y+GCGG+ N F + + C+ +C E + C T PC + + + ++ + C GC T GN FS EC+ CL+ R E A E CLL G C+G R+YY+ C SF ++GC GN NNF+SF+ C C + D I N
Sbjct: 2069 SKCFLAFEPGNCYNNVTRWFYNSAEGLCDEFVYTGCGGNANNYATEEECQNECNDAQTT----CALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFC----------IGEPVVEPSAPTYSVCAEPPEAGECDNRTTAWFYDSENMACTAFTYTGCGGNGNRFETRDQCERQCGEFKGVDVCNEPVTTGPCT---DWQTKYYFNTASQACEPFTYGGCDGT---GNRFSDLFECQTVCLAGR----EPRVGSAKEICLLPVATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQCRPEPNKQDNEIGQN 2385 HSP 4 Score: 152.14 bits (383), Expect = 1.443e-36 Identity = 107/413 (25.91%), Postives = 175/413 (42.37%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC----------------EIQIAPRCRIEHTFEPCLPS--PSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPM-FALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREV 626
C L G C L ++ ++ ++C F + GC GN NNF +LE CE C + D C P G C+ +++D Q C +F Y GCGGN N F + C + C D + + ++ + C + G+C + F++ C + Y CGG+ N F + C+ RC Q P + +P P + W Y+E + C GC N F+T EC C + + +P+ C L E G+C+ ++ R++Y + C F ++GC GN NN+ + + C C+DA+ + C P ++G C R++++ C EF + GC+G+RNNF +++ C C+ V
Sbjct: 1790 CALPKQTGDCSEKLAKWHFSESEKRCVPFYYSGCGGNKNNFPTLESCEDHCPRQVAK--DICEIPAEVGECANYVTSWYYDTQDQACRQFYYGGCGGNENRFPTEESCLARC-DRKPEPTTTTPATRPQPSRQDV--CDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRCLSQEPPAPTPPRAPAPTRQPDPAPTVAQCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGND---NRFATEEECSARCSVNIDIRIGADPVEHDTSKCFLAFEPGNCYNNVTRWFYNSAEGLCDEFVYTGCGGNANNYATEEECQNECNDAQ-----------TTCALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFCIGEPV 2183 HSP 5 Score: 145.591 bits (366), Expect = 1.875e-34 Identity = 113/412 (27.43%), Postives = 163/412 (39.56%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCS------------EHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC--EIQIAPRCRIEHTFEPCLPSP------SYTSVWVYSEEQRMCVV--TVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRD---QPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C G CD + + + YN +C++F +GGC GN N F + EEC CS EH K C G+C + ++ ++ C +FVY+GCGGN NN+ + ECQ+ C D + + C P G C + F++ +C + ++GC G+ N F+S C N C E + P T+ C P + T+ W Y E C GC GN F T +CER C + + EP+ G C ++Y+ + C FT+ GC G GN F C C GS IC P G C + R++Y+ C F Y GC G++NNF + C C
Sbjct: 2001 CSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARCSVNIDIRIGADPVEHDTSK---CFLAFEPGNCYNNVTRWFYNSAEGLCDEFVYTGCGGNANNYATEEECQNECND-------------------AQTTCALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFCIGEPVVEPS---APTYSVCAEPPEAGECDNRTTAWFYDSENMACTAFTYTGCGGN---GNRFETRDQCERQCGEFKGVDVCNEPVTT----------GPCTDWQTKYYFNTASQACEPFTYGGCDGTGNRFSDLFECQTVCLAGREPRVGSA---KEICLLPVATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQC 2371 HSP 6 Score: 106.301 bits (264), Expect = 3.475e-22 Identity = 77/270 (28.52%), Postives = 111/270 (41.11%), Query Frame = 0
Query: 372 CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESE 638
C P +G C Y F+ + C + Y GC G+DN F S CK+ C+ C + + PC +T W + ++ C GC T N F + +C+ TC + + L C E G C G+ R+YY+ + C FT+ GC GN NN+ + C C V D C PK G C ++ ++ S + C F Y GC G++NNFPT CE C + + AE E
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQDYTGKHVCLLPKSAGPCT---GFTKKWYFDVDRNRCEEFQYGGCYGTN---NRFDSLEQCQGTCAASEN----------LPTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQPGVLKD--------RCALPKQTGDCSEKLAKWHFSESEKRCVPFYYSGCGGNKNNFPTLESCEDHCPRQVAKDICEIPAEVGE 1857 HSP 7 Score: 88.9669 bits (219), Expect = 8.429e-17 Identity = 48/172 (27.91%), Postives = 82/172 (47.67%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEK----DDPCISPLLKGSCSGSG---EKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRH-----------------IFRVPKRSLSDCLKPITSGDCQ 382
C + GPC + ++++N ++ CE F +GGC G GN F L EC+T C + + + C+ P+ G C+G ++++D ++ C F+Y+GC GN NNF S C + C+ + N +G++ + RV RS +C + I C+
Sbjct: 2253 CNEPVTTGPCTDWQTKYYFNTASQACEPFTYGGCDGTGNRFSDLFECQTVCLAGREPRVGSAKEICLLPVATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQCRPEPNKQDNEIGQNPCDTFDAECQELRCPYGVRRVAARSQPECTQCICENPCE 2424
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|74960830|sp|O76840.1|PPN1_CAEEL (RecName: Full=Papilin; AltName: Full=Abnormal cell migration protein 6; Flags: Precursor) HSP 1 Score: 162.54 bits (410), Expect = 6.593e-40 Identity = 141/579 (24.35%), Postives = 228/579 (39.38%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSE------------------------------------HKPEKDDP--------------------CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD----------------------------------CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPRCRIEHTFEPC-LPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFA--LEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVAEIFIGIALSGLVILGVYFGFRLILEQVLVKEMQSHGSGLVFSDHFGQK----IELTDSFTDLFGV 713
CQL +GPC ++YN + +CE F +GGC GN N F ++EEC+ C + H +D P C + GSC +H+ S++C +F Y GC GN N F++ +C+++C ++ + S+ + + L+D C+ P G C + F+ ++QCV ++YSGC + N F S E C+ C + + C + C L P W + + C ++ GC GN FS+ ++CE C VE +++ + C L G C S+ +Y++ C FT+ GC GN N F S + C +C + +++ IC GPCR ++FY+P I++C F YGGC+G+ N F + C C + +V+ A ESE + +++S V I I + +G F R VL +G L F ++ +E+ D+ T GV
Sbjct: 1504 CQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARCPKDDQTTTTSQPEELPSLPLVQEDPQPRPAFSLKQSFAHSRRRDAPFARSVSARHHTPDSEEERVDCYAVPDPGSCGDYRLVWHYSATSNSCRQFYYGGCAGNTNRFETRDKCETSCVAKIEERVESVSEASKSLEEVRLTDPRMDSHFGYHDPEVDQIEEEAEYVIVDTGALPELCMLPEQRGSCYDNILRWRFDSEKSQCVTFMYSGCNPNANHFTSQETCERACGKWRNVAVCELPAEHGDCQLAIPR----WYHDPKTSQCQMMMWTGCGGN---GNAFSSKADCESLC--------RVETLWSNNTDFCTLERSAGPCTDSISMWYFDSTHLDCKPFTYGGCRGNQNRFVSKEQCQQSCRPGDTKSE-------DICTLRPEPGPCRLGLEKYFYDPVIQSCHMFHYGGCEGNANRFDSELDCFRRCSSVKVE------ASESERVGQLTSASTPV-----IYIVNKTAIFVGNTFRIRCNSYGVLPITWYKNGGLLQFGSRITEENDDTLEIVDALTADAGV 2049 HSP 2 Score: 119.013 bits (297), Expect = 3.367e-26 Identity = 112/478 (23.43%), Postives = 181/478 (37.87%), Query Frame = 0
Query: 216 STTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEK-----------DDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPS--PSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC----------------------------------HDAEV-----RADGSILTNAS---ICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESE 638
S +P S+S A C L + +G C ++Y T C F + GC GN N F S ++CE+ C + E + C G C+ K++++ TC++F Y GC G N FD+ +C++ C++H D C P G C + Y +N +QC + Y GC G+ N F + E C+ RC + + T P PS V + + + ++++R F+ S +S R H+ + E C P+ GSC ++Y +C F + GC GN N F++ C +C HD EV A+ I+ + +C P+ +G C + R+ ++ C F Y GC + N+F ++ CE C A + AE +
Sbjct: 1359 SASPVVVPSNSKQRDA---CHLNVDQGRCKGAFDSWYYEVATGSCVTFKYTGCGGNANRFASKDQCESLCVKPASEAASAGIDGAAGINSVCDEAKDTGPCTNFVTKWYYNKADGTCNRFHYGGCQGTNNRFDNEQQCKAACQNHKDA-------------------CQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARCP-------KDDQTTTTSQPEELPSLPLVQEDPQPRPAFSLKQSFAHSRRRDAPFARS-------VSARHHTPDSEEERV--DCYAVPDPGSCGDYRLVWHYSATSNSCRQFYYGGCAGNTNRFETRDKCETSCVAKIEERVESVSEASKSLEEVRLTDPRMDSHFGYHDPEVDQIEEEAEYVIVDTGALPELCMLPEQRGSCYDNILRWRFDSEKSQCVTFMYSGCNPNANHFTSQETCERACGKWRNVAVCELPAEHGD 1798 HSP 3 Score: 93.5893 bits (231), Expect = 3.079e-18 Identity = 99/443 (22.35%), Postives = 160/443 (36.12%), Query Frame = 0
Query: 293 DDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC----------------KDHMDPTMNSLGRHIFRVPKRSLSDCLKP------------ITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC--------------EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSI---LTNASICEQPKLKGPC-----------RRDQP---------------------------------RFFYNPSI--ETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHE 642
+D C S G C +++ ++ S C F Y GCGGN N F S EC+ C + P + +I P++S S + P + G C+ + + CV + Y+GCGG+ N F S + C++ C I C PC ++ + W Y++ C GC T N F +C+ C + + + C L +G C G +YY C +FT+ GC GN N F + + C A C + S L + + ++ P RRD P R ++ S +C +F YGGC G+ N F TR +CE +C+ + ++ ++ ++E S++ E
Sbjct: 1268 EDICRSRQDAGPCETYSDQWFYNAFSQECETFTYGGCGGNLNRFRSKDECEQRCFFVHGAQPSAARQEQAQPAAQPAQPAQPSNIVSPPQQSASPVVVPSNSKQRDACHLNVDQGRCKGAFDSWYYEVATGSCVTFKYTGCGGNANRFASKDQCESLCVKPASEAASAGIDGAAGINSVCDEAKDTGPCT---NFVTKWYYNKADGTCNRFHYGGCQGTN---NRFDNEQQCKAACQNHK------------DACQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARCPKDDQTTTTSQPEELPSLPLVQEDPQPRPAFSLKQSFAHSRRRDAPFARSVSARHHTPDSEEERVDCYAVPDPGSCGDYRLVWHYSATSNSCRQFYYGGCAGNTNRFETRDKCETSCVAK-IEERVESVSEASKSLEE 1691 HSP 4 Score: 73.559 bits (179), Expect = 4.295e-12 Identity = 90/387 (23.26%), Postives = 146/387 (37.73%), Query Frame = 0
Query: 290 PEKDDPCISPLLKGSCSGSGEK--FHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGR-HIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAP---------RCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC---HDAE-----------------VRADGSILTNASICEQPKL----------------KGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDA 628
P K C G+ G+G K +H+D C++F Y GCGGN NNF S C++ C ++P GR ++ RV D L+P Y ++ ++ CV + + GC G+ N F S E C C+ + P + + LP+P + + S EQ ++ + S E C S R + G C +++Y + C +FT+ GC GN N F+S C C H A+ +I++ P + +G C+ ++Y + +C F+Y GC G+ N F ++ QCE+ C+ +A
Sbjct: 1083 PHKIAACNQTQESGTVCGAGYKLAWHYDTTEGRCNQFWYGGCGGNDNNFASQDMCETIC---VEPP--GKGRCYLPRVDGPLRCDQLQP-----------RYYYDHSKKHCVAFWWRGCLGNANNFNSFEECSMFCK-DVGPYDAPTTAAPPPPPQQNAQQYLPTPEVQQIEIQSAEQPQPQQPQQQQQQQQQQPQQPRQSM-EDICRS-------------------RQDAGPCETYSDQWFYNAFSQECETFTYGGCGGNLNRFRSKDECEQRCFFVHGAQPSAARQEQAQPAAQPAQPAQPSNIVSPPQQSASPVVVPSNSKQRDACHLNVDQGRCKGAFDSWYYEVATGSCVTFKYTGCGGNANRFASKDQCESLCVKPASEA 1432 HSP 5 Score: 73.1738 bits (178), Expect = 5.451e-12 Identity = 46/138 (33.33%), Postives = 64/138 (46.38%), Query Frame = 0
Query: 215 PSTTPASTLSSSTSELAGSPCQLAMARGP-CDL-YLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKG--SCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD 348
P+TTP + + + + C G C Y + + Y+ +C F +GGC GN NNF S + CET C E P C P + G C ++++D C F + GC GN NNF+S EC CKD
Sbjct: 1069 PTTTPPFLMGGTVAPHKIAACNQTQESGTVCGAGYKLAWHYDTTEGRCNQFWYGGCGGNDNNFASQDMCETICVE--PPGKGRCYLPRVDGPLRCDQLQPRYYYDHSKKHCVAFWWRGCLGNANNFNSFEECSMFCKD 1204 HSP 6 Score: 65.4698 bits (158), Expect = 1.565e-9 Identity = 78/304 (25.66%), Postives = 114/304 (37.50%), Query Frame = 0
Query: 4 DQIPEGGNGSVEHVI------PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIES---------WPGRRNNSFIVMNDLVVL-QTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPE 291
DQI E E+VI PE C+L +RG C+ R+ F+ C TF +SGC PN N+F ++ CER C L E + L + + D Q++ + G N +S C R + ++ ++R+ A T S+ + +S + G R N N V Q QS RP ++ T P P C L L ++FY+ + C F +GGC+GN N F S +C CS K E
Sbjct: 1711 DQIEE----EAEYVIVDTGALPELCMLPEQRGSCYDNILRWRFDSEKSQCVTFMYSGCNPNANHFTSQETCERACGKWRNVAVCELPAE----HGDCQLAIPRWYHDPKTSQCQMMMWTGCGGNG-NAFS---SKADCESLCRVETLWSNNTDF---CTLERS-AGPCTDSISMWYFDSTHLDCKPFTYGGCRGN----QNRFVSKEQCQQSCRPGDTKSEDICTLRPEPGP------------------------CRLGLEKYFYDPVIQSCHMFHYGGCEGNANRFDSELDCFRRCSSVKVE 1970
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|125934|sp|P19761.2|TFPI1_RABIT (RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor) HSP 1 Score: 138.658 bits (348), Expect = 3.151e-35 Identity = 80/221 (36.20%), Postives = 116/221 (52.49%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPE----------KDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCK---------DH---MDPTMNSLGRHIFRVPKRSL----SDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR 429
C + + GPC Y+ RFF+N T +CE F++GGC+GN N F+SLEEC+ C+ P+ K D C G C G ++ ++ QS C +F Y GC GN NNF+SL EC++TC+ DH ++ N+L + P+R S CL P G CQ + + +N +C P+ YSGCGG++N F S +AC C+ P+
Sbjct: 50 CAMKVDDGPCRAYIKRFFFNILTHQCEEFIYGGCEGNENRFESLEECKEKCARDYPKMTTKLTFQKGKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNLNNFESLEECKNTCENPTSDFQVDDHRTQLNTVNNTLINQPTKAPRRWAFHGPSWCLPPADRGLCQANEIRFFYNAIIGKCRPFKYSGCGGNENNFTSKKACITACKKGFIPK 270 HSP 2 Score: 83.5741 bits (205), Expect = 2.268e-16 Identity = 52/177 (29.38%), Postives = 76/177 (42.94%), Query Frame = 0
Query: 472 NEFSTSSECERTCLSDRSHSLEVEPMFAL---EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC-----------HDAEVRADGSILTN-------------ASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
N F + EC+ C D + + F + C L + G C G + R++Y + C F + GC GN NNF+S + C TC H ++ + L N S C P +G C+ ++ RFFYN I C F+Y GC G+ NNF ++ C C
Sbjct: 88 NRFESLEECKEKCARDYP-KMTTKLTFQKGKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNLNNFESLEECKNTCENPTSDFQVDDHRTQLNTVNNTLINQPTKAPRRWAFHGPSWCLPPADRGLCQANEIRFFYNAIIGKCRPFKYSGCGGNENNFTSKKACITAC 263 HSP 3 Score: 77.0258 bits (188), Expect = 3.821e-14 Identity = 40/128 (31.25%), Postives = 60/128 (46.88%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSIL---TNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
C ++ + G C + RF++ + C F + GC GN N F+S + C C + + C + G CR R+FYN + CE F+YGGC G+ NNF + +C+ TC N D
Sbjct: 50 CAMKVDDGPCRAYIKRFFFNILTHQCEEFIYGGCEGNENRFESLEECKEKCARDYPKMTTKLTFQKGKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNLNNFESLEECKNTCENPTSD 177 HSP 4 Score: 74.3294 bits (181), Expect = 3.514e-13 Identity = 62/229 (27.07%), Postives = 96/229 (41.92%), Query Frame = 0
Query: 366 KRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPS-----------PSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTC---------------LSDRSHSLEVEPM-----FALEG---CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEV 558
K + S C + G C+ + FN +QC +IY GC G++N F S E CK +C + P+ + TF+ P Y + + Y+ + + C GC N F + EC+ TC L+ +++L +P +A G CL +RG C + RF+Y + C F +SGC GN NNF S + C+ C +
Sbjct: 44 KPTHSFCAMKVDDGPCRAYIKRFFFNILTHQCEEFIYGGCEGNENRFESLEECKEKC-ARDYPKMTTKLTFQKGKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNL---NNFESLEECKNTCENPTSDFQVDDHRTQLNTVNNTLINQPTKAPRRWAFHGPSWCLPPADRGLCQANEIRFFYNAIIGKCRPFKYSGCGGNENNFTSKKACITACKKGFI 268 HSP 5 Score: 56.6102 bits (135), Expect = 2.051e-7 Identity = 28/57 (49.12%), Postives = 33/57 (57.89%), Query Frame = 0
Query: 19 PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNG 75
P +CL +RG C E R+F+N G C F +SGCG NENNF KKAC C G
Sbjct: 210 PSWCLPPADRGLCQANEIRFFYNAIIGKCRPFKYSGCGGNENNFTSKKACITACKKG 266
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|125932|sp|P10646.1|TFPI1_HUMAN (RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor) HSP 1 Score: 129.413 bits (324), Expect = 6.622e-32 Identity = 76/227 (33.48%), Postives = 111/227 (48.90%), Query Frame = 0
Query: 229 ELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH----------KPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD------------HMDPTMNSLGRHIFRVPK----RSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR 429
+L S C GPC + RFF+N T +CE F++GGC+GN N F+SLEEC+ C+ + EK D C G C G ++ ++ Q+ C +F Y GC GN NNF++L EC++ C+D ++ NSL +VP S CL P G C+ + + +N +C P+ YSGCGG++N F S + C C+ R
Sbjct: 48 KLMHSFCAFKADDGPCKAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMCTRDNANRIIKTTLQQEKPDFCFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDGPNGFQVDNYGTQLNAVNNSLTPQSTKVPSLFEFHGPSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRACKKGFIQR 274 HSP 2 Score: 90.1225 bits (222), Expect = 1.619e-18 Identity = 73/261 (27.97%), Postives = 102/261 (39.08%), Query Frame = 0
Query: 303 GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD-CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG---CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVR 559
G C ++F F+ + C +F+Y GC GN N F+SL EC+ C T ++ R I ++ D C G C+ Y +N QC + Y GC G+ N F + E CKN CE P+ V Y + + N + S +V +F G CL +RG C + RFYY + C F +SGC GN NNF S Q CL C ++
Sbjct: 61 GPCKAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMC------TRDNANRIIKTTLQQEKPDFCFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICE-----------------DGPNGFQVDNYG------------TQLNAVNNSLT-------------PQSTKVPSLFEFHGPSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRACKKGFIQ 273 HSP 3 Score: 88.5817 bits (218), Expect = 5.458e-18 Identity = 55/194 (28.35%), Postives = 87/194 (44.85%), Query Frame = 0
Query: 472 NEFSTSSECERTCLSDRSHSL------EVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA-----------EVRADGSILT-------------NASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAE 635
N F + EC++ C D ++ + + +P F C L + G C G + R++Y + C F + GC GN NNF++ + C C D ++ A + LT S C P +G CR ++ RF+YN I C F+Y GC G+ NNF ++ +C C FIQ +++
Sbjct: 92 NRFESLEECKKMCTRDNANRIIKTTLQQEKPDF----CFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDGPNGFQVDNYGTQLNAVNNSLTPQSTKVPSLFEFHGPSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRAC----KKGFIQRISK 277 HSP 4 Score: 56.225 bits (134), Expect = 2.864e-7 Identity = 26/57 (45.61%), Postives = 33/57 (57.89%), Query Frame = 0
Query: 19 PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNG 75
P +CL +RG C E R+++N G C F +SGCG NENNF K+ C R C G
Sbjct: 214 PSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRACKKG 270
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|6094466|sp|Q28864.1|TFPI1_MACMU (RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor) HSP 1 Score: 127.102 bits (318), Expect = 3.872e-31 Identity = 80/251 (31.87%), Postives = 118/251 (47.01%), Query Frame = 0
Query: 199 SLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH----------KPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD------------HMDPTMNSLGRHIFRVPK----RSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCE 423
+L P+ + DS + + L +L S C GPC + RFF+N T +CE F++GGC GN N F+S+EEC+ C+ + EK D C G C G ++ ++ QS C +F Y GC GN NNF++L EC++TC+D ++ NS +VP S CL P G C+ + + +N +C P+ YSGCGG++N F S C C+
Sbjct: 20 NLAPAPLNADSEEDEEYTIITDTELPPL--KLMHSFCAFKPDDGPCKAIMKRFFFNIFTRQCEEFIYGGCGGNQNRFESMEECKKVCTRDNVHRIIQTALQQEKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNMNNFETLEECKNTCEDGLNGFQVDNYGTQLNAVNNSQTPQSTKVPSFFEFHGPSWCLAPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKRECLRACK 268 HSP 2 Score: 92.4337 bits (228), Expect = 2.762e-19 Identity = 56/191 (29.32%), Postives = 85/191 (44.50%), Query Frame = 0
Query: 472 NEFSTSSECERTCLSDRSHSLEVEPMFALEG---CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA----EVRADGSILTNA--------------------SICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAE 635
N F + EC++ C D H + ++ E C L + G C G + R++Y + C F + GC GN NNF++ + C TC D +V G+ L S C P +G CR ++ RF+YN I C F+Y GC G+ NNF ++ +C C FIQ +++
Sbjct: 92 NRFESMEECKKVCTRDNVHRI-IQTALQQEKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNMNNFETLEECKNTCEDGLNGFQVDNYGTQLNAVNNSQTPQSTKVPSFFEFHGPSWCLAPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKRECLRAC----KKGFIQRISK 277 HSP 3 Score: 83.1889 bits (204), Expect = 3.618e-16 Identity = 44/125 (35.20%), Postives = 60/125 (48.00%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKL------KGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C +P+ G C + RF++ C F + GC GN N F+S + C C V I+ A E+P G CR R+FYN + CE F+YGGC G+ NNF T +C+ TC
Sbjct: 54 CAFKPDDGPCKAIMKRFFFNIFTRQCEEFIYGGCGGNQNRFESMEECKKVCTRDNVH---RIIQTALQQEKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNMNNFETLEECKNTC 175 HSP 4 Score: 56.6102 bits (135), Expect = 2.266e-7 Identity = 26/57 (45.61%), Postives = 33/57 (57.89%), Query Frame = 0
Query: 19 PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNG 75
P +CL +RG C E R+++N G C F +SGCG NENNF K+ C R C G
Sbjct: 214 PSWCLAPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKRECLRACKKG 270
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|401174|sp|Q02445.1|TFPI1_RAT (RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor) HSP 1 Score: 117.087 bits (292), Expect = 1.255e-27 Identity = 66/220 (30.00%), Postives = 105/220 (47.73%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC----------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTM------------------NSLGRHIFRVPKRSLSD----CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
C + GPC + +++N + +CE F++GGC+GN N F +LEEC TC + EK D C G C G ++ ++ QS C +F Y GC GN NNF++L EC++TC+D ++ N + + P + D CL+P SG C+ + + +N +C + Y+GCGG++N F + + C C
Sbjct: 53 CAMKAEDGPCKAMIRSYYFNMNSHQCEEFIYGGCRGNKNRFDTLEECRKTCIPGYKKTTIKTTSGAEKPDFCFLEEDPGICRGFMTRYFYNNQSKQCEQFKYGGCLGNSNNFETLEECRNTCEDPVNEVQKGDYVTNQITVTDRTTVNNVVIPQATKAPSQWDYDGPSWCLEPADSGLCKASEKRFYYNPAIGKCRQFNYTGCGGNNNNFTTKQDCNRAC 272 HSP 2 Score: 93.9745 bits (232), Expect = 7.901e-20 Identity = 60/195 (30.77%), Postives = 86/195 (44.10%), Query Frame = 0
Query: 463 GCSNTKRIGNEFSTSSECERTCL------SDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA--EVR-----------ADGSILTNASI-----------------CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
GC K N F T EC +TC+ + ++ S +P F C L + G C G + R++Y + C F + GC GN NNF++ + C TC D EV+ D + + N I C +P G C+ + RF+YNP+I C +F Y GC G+ NNF T+ C C
Sbjct: 85 GCRGNK---NRFDTLEECRKTCIPGYKKTTIKTTSGAEKPDF----CFLEEDPGICRGFMTRYFYNNQSKQCEQFKYGGCLGNSNNFETLEECRNTCEDPVNEVQKGDYVTNQITVTDRTTVNNVVIPQATKAPSQWDYDGPSWCLEPADSGLCKASEKRFYYNPAIGKCRQFNYTGCGGNNNNFTTKQDCNRAC 272 HSP 3 Score: 82.0333 bits (201), Expect = 7.825e-16 Identity = 49/168 (29.17%), Postives = 75/168 (44.64%), Query Frame = 0
Query: 493 EVEPMFALEG-CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKL------KGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVAE 653
E+ PM L C ++ E G C + +Y+ C F + GC GN N F + + C TC + + + S E+P G CR R+FYN + CE+F+YGGC G+ NNF T +C TC + + T + +T + VV+ +
Sbjct: 42 ELRPMKPLHTFCAMKAEDGPCKAMIRSYYFNMNSHQCEEFIYGGCRGNKNRFDTLEECRKTCIPGYKK---TTIKTTSGAEKPDFCFLEEDPGICRGFMTRYFYNNQSKQCEQFKYGGCLGNSNNFETLEECRNTCEDPVNEVQKGDYVTNQITVTDRTTVNNVVIPQ 206 HSP 4 Score: 81.6481 bits (200), Expect = 1.257e-15 Identity = 47/163 (28.83%), Postives = 75/163 (46.01%), Query Frame = 0
Query: 221 STLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSE-----------------------------------HKPEKDDP--CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
+T+ +++ C L G C ++ R+FYN ++++CE F +GGC GN NNF++LEEC TC + + + D P C+ P G C S ++F+++ C +F Y+GCGGN NNF + +C C
Sbjct: 110 TTIKTTSGAEKPDFCFLEEDPGICRGFMTRYFYNNQSKQCEQFKYGGCLGNSNNFETLEECRNTCEDPVNEVQKGDYVTNQITVTDRTTVNNVVIPQATKAPSQWDYDGPSWCLEPADSGLCKASEKRFYYNPAIGKCRQFNYTGCGGNNNNFTTKQDCNRAC 272
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|13124581|sp|O54819.1|TFPI1_MOUSE (RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor) HSP 1 Score: 115.931 bits (289), Expect = 2.999e-27 Identity = 71/258 (27.52%), Postives = 115/258 (44.57%), Query Frame = 0
Query: 207 TDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC----------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD--HMDPTMNSLGRHIFRVPKRSLSD--------------------------CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQI 426
D T + L S P T C + GPC + +F+N T +CE F++GGC+GN N F +LEEC+ TC + E+ D C G C G +++ ++ Q+ C +FVY GC GN NNF++L EC+ C++ H +N + + +++D CL+P SG C+ + + +N +C + Y+GCGG++N F + C C+ +
Sbjct: 33 ADDTDSELGSMKPLHTF-----------CAMKADDGPCKAMIRSYFFNMYTHQCEEFIYGGCEGNENRFDTLEECKKTCIPGYEKTAVKAASGAERPDFCFLEEDPGLCRGYMKRYLYNNQTKQCERFVYGGCLGNRNNFETLDECKKICENPVHSPSPVNEVQMSDYVTDGNTVTDRSTVNNIVVPQSPKVPRRRDYRGRPWCLQPADSGLCKASERRFYYNSATGKCHRFNYTGCGGNNNNFTTRRRCLRSCKTGL 279 HSP 2 Score: 84.3445 bits (207), Expect = 1.819e-16 Identity = 54/192 (28.12%), Postives = 80/192 (41.67%), Query Frame = 0
Query: 472 NEFSTSSECERTCL------SDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHD-------------AEVRADGSILTNASI-----------------------CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
N F T EC++TC+ + ++ S P F C L + G C G + R+ Y + C F + GC GN NNF++ C C + ++ DG+ +T+ S C QP G C+ + RF+YN + C F Y GC G+ NNF TR +C +C
Sbjct: 88 NRFDTLEECKKTCIPGYEKTAVKAASGAERPDF----CFLEEDPGLCRGYMKRYLYNNQTKQCERFVYGGCLGNRNNFETLDECKKICENPVHSPSPVNEVQMSDYVTDGNTVTDRSTVNNIVVPQSPKVPRRRDYRGRPWCLQPADSGLCKASERRFYYNSATGKCHRFNYTGCGGNNNNFTTRRRCLRSC 275 HSP 3 Score: 76.2554 bits (186), Expect = 8.552e-14 Identity = 40/127 (31.50%), Postives = 59/127 (46.46%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKL------KGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
C ++ + G C + +++ C F + GC GN N F + + C TC + + + AS E+P G CR R+ YN + CE F YGGC G+RNNF T +C+ C N
Sbjct: 50 CAMKADDGPCKAMIRSYFFNMYTHQCEEFIYGGCEGNENRFDTLEECKKTCIPG---YEKTAVKAASGAERPDFCFLEEDPGLCRGYMKRYLYNNQTKQCERFVYGGCLGNRNNFETLDECKKICEN 173
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|124015206|sp|P84875.1|PCPI_SABMA (RecName: Full=Carboxypeptidase inhibitor SmCI) HSP 1 Score: 103.99 bits (258), Expect = 1.443e-24 Identity = 60/190 (31.58%), Postives = 90/190 (47.37%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
S C L RG C Y+ ++F+N TE CE FV+GGC+GN N F++ ++C C + P K PC S + + ++ C F+Y GC GN N F + ECQ C + P C +P +G C+ Y ++ + +C +IY GC G+ N F + E+C+N C
Sbjct: 2 SVCDLPADRGQCTAYIPQWFFNKTTEDCEKFVYGGCQGNANRFETKDDCIANCGCNLPSKVGPCRV---------SARMWFHNPETEKCEVFIYGGCHGNANRFATETECQEVCDRYQKPGF-----------------CYQPSETGPCKGSFPRYYYDYEDGECKEFIYGGCEGNANNFETKESCENAC 165 HSP 2 Score: 93.2041 bits (230), Expect = 8.707e-21 Identity = 45/127 (35.43%), Postives = 70/127 (55.12%), Query Frame = 0
Query: 220 ASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
A+ + +A C L GPC + +F+N +TEKCE F++GGC GN N F + EC+ C + +K C P G C GS ++++D++ C +F+Y GC GN NNF++ C++ C
Sbjct: 41 ANRFETKDDCIANCGCNLPSKVGPCRVSARMWFHNPETEKCEVFIYGGCHGNANRFATETECQEVCDRY--QKPGFCYQPSETGPCKGSFPRYYYDYEDGECKEFIYGGCEGNANNFETKESCENAC 165 HSP 3 Score: 83.1889 bits (204), Expect = 2.222e-17 Identity = 59/187 (31.55%), Postives = 82/187 (43.85%), Query Frame = 0
Query: 369 LSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
+S C P G C + FN+T C ++Y GC G+ N F + + C C C + PC S +W ++ E C V + GC N F+T +EC+ C DR +P F C E G C GS PR+YY+ D C F + GC GN NNF++ + C C
Sbjct: 1 ISVCDLPADRGQCTAYIPQWFFNKTTEDCEKFVYGGCQGNANRFETKDDCIANC------GCNLPSKVGPCRVS---ARMWFHNPETEKCEVFIYGGCHGN---ANRFATETECQEVC--DRYQ----KPGF----CYQPSETGPCKGSFPRYYYDYEDGECKEFIYGGCEGNANNFETKESCENAC 165 HSP 4 Score: 82.8037 bits (203), Expect = 3.704e-17 Identity = 54/180 (30.00%), Postives = 74/180 (41.11%), Query Frame = 0
Query: 444 SYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
+Y W +++ C V GC N F T +C C GC L + G C S +++ P E C F + GCHGN N F + C C + C QP GPC+ PR++Y+ C+EF YGGC+G+ NNF T+ CE C
Sbjct: 15 AYIPQWFFNKTTEDCEKFVYGGCQGN---ANRFETKDDCIANC-----------------GCNLPSKVGPCRVSARMWFHNPETEKCEVFIYGGCHGNANRFATETECQEVCDRYQ---------KPGFCYQPSETGPCKGSFPRYYYDYEDGECKEFIYGGCEGNANNFETKESCENAC 165 HSP 5 Score: 82.0333 bits (201), Expect = 6.734e-17 Identity = 42/127 (33.07%), Postives = 58/127 (45.67%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAF 629
C L +RG C +P++++ E C F + GC GN N F++ C+A C C P GPCR +F+NP E CE F YGGC G+ N F T +C+ C + F
Sbjct: 4 CDLPADRGQCTAYIPQWFFNKTTEDCEKFVYGGCQGNANRFETKDDCIANCG----------------CNLPSKVGPCRVSARMWFHNPETEKCEVFIYGGCHGNANRFATETECQEVCDRYQKPGF 114
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|1351226|sp|P48307.1|TFPI2_HUMAN (RecName: Full=Tissue factor pathway inhibitor 2; Short=TFPI-2; AltName: Full=Placental protein 5; Short=PP5; Flags: Precursor) HSP 1 Score: 105.916 bits (263), Expect = 1.914e-24 Identity = 63/186 (33.87%), Postives = 84/186 (45.16%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNG--NNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEAC 418
C L + GPC L+R++Y+ T+ C F++GGC+GN NNF + E C+ C + + + C GS EK+ F+ S TC KF GC N N F A C C PK+ S C P G C Y FN C + Y+GCGG+DN F+S E C
Sbjct: 36 CLLPLDYGPCRALLLRYYYDRYTQSCRQFLYGGCEGNANNFYTWEACDDACWRIEKVPKVCRLQVSVDDQCEGSTEKYFFNLSSMTCEKFFSGGCHRNRIENRFPDEATCMGFC-----------------APKKIPSFCYSPKDEGLCSANVTRYYFNPRYRTCDAFTYTGCGGNDNNFVSREDC 204 HSP 2 Score: 72.4034 bits (176), Expect = 4.864e-13 Identity = 51/182 (28.02%), Postives = 83/182 (45.60%), Query Frame = 0
Query: 372 CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPR-CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHC 549
CL P+ G C+ Y +++ C ++Y GC G+ N F + EAC + C I+ P+ CR++ + + T + ++ C + GC + RI N F + C C + S P + G C ++ R+Y+ P TC +FT++GC GN NNF S + C
Sbjct: 36 CLLPLDYGPCRALLLRYYYDRYTQSCRQFLYGGCEGNANNFYTWEACDDACWRIEKVPKVCRLQVSVDD--QCEGSTEKYFFNLSSMTCEKFFSGGC-HRNRIENRFPDEATCMGFCAPKKIPSFCYSPK----------DEGLCSANVTRYYFNPRYRTCDAFTYTGCGGNDNNFVSREDC 204 HSP 3 Score: 71.633 bits (174), Expect = 1.034e-12 Identity = 43/132 (32.58%), Postives = 63/132 (47.73%), Query Frame = 0
Query: 501 EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICE-QPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSR--NNFPTRAQCEATCLNREVDAF 629
E CLL + G C L R+YY+ ++C F + GC GN NNF +++ C C E +C Q + C ++F+N S TCE+F GGC +R N FP A C C +++ +F
Sbjct: 34 EICLLPLDYGPCRALLLRYYYDRYTQSCRQFLYGGCEGNANNFYTWEACDDACWRIE--------KVPKVCRLQVSVDDQCEGSTEKYFFNLSSMTCEKFFSGGCHRNRIENRFPDEATCMGFCAPKKIPSF 157 HSP 4 Score: 62.3882 bits (150), Expect = 1.393e-9 Identity = 35/108 (32.41%), Postives = 50/108 (46.30%), Query Frame = 0
Query: 512 CHGSLPRFYYEPMDETCHSFTFSGCHGNG--NNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQC 617
C GS ++++ TC F GCH N N F C+ C ++ S C PK +G C + R+++NP TC+ F Y GC G+ NNF +R C
Sbjct: 106 CEGSTEKYFFNLSSMTCEKFFSGGCHRNRIENRFPDEATCMGFCAPKKI---------PSFCYSPKDEGLCSANVTRYYFNPRYRTCDAFTYTGCGGNDNNFVSREDC 204
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|82203423|sp|Q6T269.1|VKT3_BITGA (RecName: Full=Kunitz-type serine protease inhibitor bitisilin-3; AltName: Full=Two-Kunitz protease inhibitor) HSP 1 Score: 101.679 bits (252), Expect = 6.098e-24 Identity = 54/136 (39.71%), Postives = 71/136 (52.21%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD------HMDPTMNSLGRHIFRV 364
C L GPC Y RF+Y+ + KCE F++GGC GN NNFK+ EEC TC E CI P G C +F++D S+ C F+Y GC GN NNF + EC+ TC + + P GR + R+
Sbjct: 18 CNLPADLGPCKNYTGRFYYDSASNKCEVFIYGGCPGNANNFKTREECRKTCVEI-------CILPAELGPCDEYTGRFYYDSASNKCEVFIYGGCQGNANNFKTRDECRKTCVEICILPAELGPCDEYTGRLLLRL 146 HSP 2 Score: 86.6557 bits (213), Expect = 1.212e-18 Identity = 47/125 (37.60%), Postives = 60/125 (48.00%), Query Frame = 0
Query: 498 FALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
F E C L + G C RFYY+ C F + GC GN NNF++ + C TC IC P GPC RF+Y+ + CE F YGGC+G+ NNF TR +C TC+
Sbjct: 13 FPAEFCNLPADLGPCKNYTGRFYYDSASNKCEVFIYGGCPGNANNFKTREECRKTC--------------VEICILPAELGPCDEYTGRFYYDSASNKCEVFIYGGCQGNANNFKTRDECRKTCV 123 HSP 3 Score: 68.5514 bits (166), Expect = 2.012e-12 Identity = 41/134 (30.60%), Postives = 59/134 (44.03%), Query Frame = 0
Query: 296 CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAP 428
C P G C +F++D S+ C F+Y GC GN NNF + EC+ TC + C+ P G C G + ++ N+C +IY GC G+ N F + + C+ C EI I P
Sbjct: 18 CNLPADLGPCKNYTGRFYYDSASNKCEVFIYGGCPGNANNFKTREECRKTC----------------------VEICILPAELGPCDEYTGRFYYDSASNKCEVFIYGGCQGNANNFKTRDECRKTCVEICILP 129
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|669301659|gb|KFD45427.1| (hypothetical protein M513_13698 [Trichuris suis] >gi|669328879|gb|KFD69066.1| hypothetical protein M514_13698 [Trichuris suis]) HSP 1 Score: 244.973 bits (624), Expect = 2.033e-66 Identity = 181/610 (29.67%), Postives = 267/610 (43.77%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR--CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
C L RG C Y +++N+ S C+ F++ GC N N F ++ CE C+ G EE + +L + WD +Y N S C K +P + L E+ P R SFI Q+ Q + S ++ +PC+L GPC IR++YNW ++C F +GGC+GN NNF+S+ +CE TC++ K +KD+PC P G C S +++++ QS C FVY GC GN N+F++L +C++ C +H N C P +G C+ Y +N+ +C ++Y GC G++N F S E C+ +C Q C + PC S + + Y + + CV GC N F +++ECE TC ++R +S + C L + G C GSL RFYY C +F + GC GN NNF+S Q+C A C G T S CE P GPC RF+YN + + CE F YGGC+G+ NNF + +CEA C ++ +
Sbjct: 84 CELPAVRGRCKAYIVVFYYNKESKKCDKFAYGGCEGNGNRFTTQEQCEAKCV-GQKKEENPCKLPAETGPCKASLT--RYHYAWDLKKCVAFTYGGCKGNSNNFESIAECEEKCG----------KKEKDENPCE---------------LPAETGP--RMASFIRF--YYDKQSKQCKTFTYGGRQGNANNFESLQECEARCVDQNKKEENPCKLRADPGPCKAVFIRYYYNWDWKECMKFTYGGCEGNANNFESIAQCEATCADKK-KKDNPCDLPAEPGPCRASFIRYYYNKQSKKCETFVYGGCEGNENSFETLEDCEARCIEHKKTEKNP---------------CDLPADAGPCKASIPRYYYNKNSKKCDTFVYGGCHGNENNFGSLEECQAKCAEQKKQENPCMLPADSGPCKAS---FTKYYYDWDLKKCVTFTYGGCQGN---ANNFESAAECEATC-AERKNSDKGP-------CELPADTGPCDGSLIRFYYNKDAGKCKTFKYGGCLGNQNNFESLQNCEAKCA-------GQKKTQQSPCELPAETGPCMASFIRFYYNKASKKCETFTYGGCQGNENNFESLQECEAKCAEQKKE 624 HSP 2 Score: 226.483 bits (576), Expect = 8.157e-60 Identity = 170/600 (28.33%), Postives = 260/600 (43.33%), Query Frame = 0
Query: 37 RYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEE--ILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTI---ATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC----EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
R+++++ S C+TF++ G N NNF + CE C++ N EE LR +P F + + +WD + +Y N S +CA + K +P + + PG SFI QS + T + + + + +PC L GPC + R++YN ++KC+ FV+GGC GN NNF SLEEC+ C+E K ++++PC+ P G C S K+++D+ C F Y GC GN NNF+S AEC++TC + + C P +G C + +N+ +C + Y GC G+ N F S + C+ +C + Q +P C + PC+ S + Y++ + C GC + N F + ECE C + E P C LR + G C R+YY + C FT+ GC GN NNF+S C ATC D + + + C+ P GPC R++YN + CE F YGGC+G+ N+F T CEA C+ ++
Sbjct: 219 RFYYDKQSKQCKTFTYGGRQGNANNFESLQECEARCVDQNKKEENPCKLRADPGP----CKAVFIRYYYNWDWKECMKFTYGGCEGNANNFESIAQCEATCADKKK-----KDNP----------------------CDLPAEPGPCRASFIRY-----YYNKQSKKCETFVYGGCEGNENSFETLEDCEARCIEHKKTEKNPCDLPADAGPCKASIPRYYYNKNSKKCDTFVYGGCHGNENNFGSLEECQAKCAEQK-KQENPCMLPADSGPCKASFTKYYYDWDLKKCVTFTYGGCQGNANNFESAAECEATCAERKN---------------SDKGPCELPADTGPCDGSLIRFYYNKDAGKCKTFKYGGCLGNQNNFESLQNCEAKCAGQKKTQQSP-CELPAETGPCMAS---FIRFYYNKASKKCETFTYGGCQGNE---NNFESLQECEAKCAEQKK---EENP------CKLRADAGPCKAIFVRYYYNWDWKECLKFTYGGCEGNANNFESIAQCEATCADKKKKDN--------PCDLPAEPGPCMASFIRYYYNKESKKCETFTYGGCEGNENSFETLKDCEARCVKQK 742 HSP 3 Score: 219.164 bits (557), Expect = 3.130e-57 Identity = 171/609 (28.08%), Postives = 257/609 (42.20%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPR--CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVV--TVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C L E G C RY + C F++ GC N NNF CE C G ++E + P + + F + + D + + +Y N S C VD+N+ L+ + PG FI + ++ + + S + + + +PC L GPC IR++YN +++KCE FV+GGC+GN N+F++LE+CE C EHK + +PC P G C S +++++ S C FVY GC GN NNF SL ECQ+ C + K+ + C+ P SG C+ Y ++ +CV + Y GC G+ N F S C+ C E + + + C + PC S + Y+++ C GC + N F + CE C + C L E G C S RFYY + C +FT+ GC GN NNF+S Q C A C + + + L + GPC+ R++YN + C +F YGGC+G+ NNF + AQCEATC +++
Sbjct: 144 CKLPAETGPCKASLTRYHYAWDLKKCVAFTYGGCKGNSNNFESIAECEEKC--GKKEKDENPCELPAETGPRMA-SFIRFYYDKQSKQCKTFTYGGRQGNANNFESLQECEARC---------------------VDQNKKEENPCKLR-----ADPGPCKAVFIRY--YYNWDWKECMKFTYGGCEGNANNFESIAQCEA-TCADKKKKDNPCDLPAEPGPCRASFIRYYYNKQSKKCETFVYGGCEGNENSFETLEDCEARCIEHKKTEKNPCDLPADAGPCKASIPRYYYNKNSKKCDTFVYGGCHGNENNFGSLEECQAKCAEQ----------------KKQENPCMLPADSGPCKASFTKYYYDWDLKKCVTFTYGGCQGNANNFESAAECEATCAERKNSDKGPCELPADTGPCDGS---LIRFYYNKDAGKCKTFKYGGCLGNQ---NNFESLQNCEAKCAGQKKTQQSP--------CELPAETGPCMASFIRFYYNKASKKCETFTYGGCQGNENNFESLQECEAKCAEQKKEENPCKLRADA--------GPCKAIFVRYYYNWDWKECLKFTYGGCEGNANNFESIAQCEATCADKK 682 HSP 4 Score: 208.379 bits (529), Expect = 1.546e-53 Identity = 129/395 (32.66%), Postives = 190/395 (48.10%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPC-LPS---PSYTS-VWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
+PC+L G C R++Y+W +++C F +GGC+GN NN+ SLEEC+ TC + +DPC P ++G C F+++ +S C KF Y GC GNGN F + +C++ C +G+ K+ + C P +G C+ Y + +CV + Y GC G+ N F S C+ +C + E PC LP+ P S + Y ++Q T + N F + ECE C+ + E P C LR + G C R+YY + C FT+ GC GN NNF+S C ATC D + + + C+ P GPCR R++YN + CE F YGGC+G+ N+F T CEA C+
Sbjct: 21 NPCKLPADTGACKGSFPRYYYDWNSKQCRQFTYGGCQGNANNYASLEECKATCPGEEQNDEDPCELPAVRGRCKAYIVVFYYNKESKKCDKFAYGGCEGNGNRFTTQEQCEAKC----------VGQ------KKEENPCKLPAETGPCKASLTRYHYAWDLKKCVAFTYGGCKGNSNNFESIAECEEKCG-------KKEKDENPCELPAETGPRMASFIRFYYDKQSKQCKTFTYGGRQGNANNFESLQECEARCVD--QNKKEENP------CKLRADPGPCKAVFIRYYYNWDWKECMKFTYGGCEGNANNFESIAQCEATCADKKKKDN--------PCDLPAEPGPCRASFIRYYYNKQSKKCETFVYGGCEGNENSFETLEDCEARCI 376 HSP 5 Score: 162.925 bits (411), Expect = 4.967e-38 Identity = 108/343 (31.49%), Postives = 160/343 (46.65%), Query Frame = 0
Query: 291 EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC----KDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR--CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
+K++PC P G+C GS ++++D+ S C +F Y GC GN NN+ SL EC++TC ++ DP C P G C+ V+ +N+ +C + Y GC G+ N F + E C+ +C Q C++ PC S + + Y+ + + CV GC K N F + +ECE C C L E G S RFYY+ + C +FT+ G GN NNF+S Q C A C D + + A GPC+ R++YN + C +F YGGC+G+ NNF + AQCEATC +++
Sbjct: 18 QKENPCKLPADTGACKGSFPRYYYDWNSKQCRQFTYGGCQGNANNYASLEECKATCPGEEQNDEDP-------------------CELPAVRGRCKAYIVVFYYNKESKKCDKFAYGGCEGNGNRFTTQEQCEAKCVGQKKEENPCKLPAETGPCKAS---LTRYHYAWDLKKCVAFTYGGC---KGNSNNFESIAECEEKCGKKEKDE---------NPCELPAETGPRMASFIRFYYDKQSKQCKTFTYGGRQGNANNFESLQECEARCVDQNKKEENPCKLRADP-------GPCKAVFIRYYYNWDWKECMKFTYGGCEGNANNFESIAQCEATCADKK 319 HSP 6 Score: 102.064 bits (253), Expect = 1.274e-18 Identity = 48/116 (41.38%), Postives = 74/116 (63.79%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEK--DDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
+PC L GPC IR++YN +++KCE F +GGC+GN N+F++L++CE C + K K + PC P G C S +++++ S C +F Y GC GN NNF+S+ +C++TC
Sbjct: 686 NPCDLPAEPGPCMASFIRYYYNKESKKCETFTYGGCEGNENSFETLKDCEARCVKQKKRKREESPCKLPADIGPCKMSIVRYYYNNGSKKCEQFTYGGCHGNENNFESMEKCEATC 801 HSP 7 Score: 89.7373 bits (221), Expect = 1.083e-14 Identity = 67/264 (25.38%), Postives = 107/264 (40.53%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC 285
C L E G C R+++N++S CETF++ GC NENNF + CE C E + K + + + + +WD + +Y N S +CA + K +P + + PG SFI + ++ + + A + + SPC+L GPC + ++R++YN ++KCE F +GGC GN NNF+S+E+CE TC
Sbjct: 568 CELPAETGPCMASFIRFYYNKASKKCETFTYGGCQGNENNFESLQECEAKCAEQKKEENPCKLRADAGPCKAIFVRY---YYNWDWKECLKFTYGGCEGNANNFESIAQCEATCADKKK-----KDNP----------------------CDLPAEPGPCMASFIRYYYNKESKKCETFTYGGCEGNENSFETLKDCEARCVKQKKRKREESPCKLPADIGPCKMSIVRYYYNNGSKKCEQFTYGGCHGNENNFESMEKCEATC 801 HSP 8 Score: 85.1149 bits (209), Expect = 2.661e-13 Identity = 49/157 (31.21%), Postives = 72/157 (45.86%), Query Frame = 0
Query: 471 GNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
N +++ EC+ TC + + + C L RG C + FYY + C F + GC GNGN F + + C A C + + C+ P GPC+ R+ Y ++ C F YGGCKG+ NNF + A+CE C +E D
Sbjct: 60 ANNYASLEECKATCPGEEQNDEDP--------CELPAVRGRCKAYIVVFYYNKESKKCDKFAYGGCEGNGNRFTTQEQCEAKCVGQKKEEN--------PCKLPAETGPCKASLTRYHYAWDLKKCVAFTYGGCKGNSNNFESIAECEEKCGKKEKD 200
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|730370971|gb|KHJ42564.1| (Kunitz/Bovine pancreatic trypsin inhibitor domain protein [Trichuris suis]) HSP 1 Score: 236.498 bits (602), Expect = 3.040e-62 Identity = 141/399 (35.34%), Postives = 206/399 (51.63%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR--CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
+PC+L GPC IR++YNW ++C F +GGC+GN NNF+S+ +CE TC++ K +KD+PC P G C S +++++ QS C FVY GC GN N+F++L +C++ C +H N C P +G C+ Y +N+ +C ++Y GC G++N F S E C+ +C Q C + PC S + + Y + + CV GC N F +++ECE TC ++R +S + C L + G C GSL RFYY C +F + GC GN NNF+S Q+C A C G T S CE P GPC RF+YN + + CE F YGGC+G+ NNF + +CEA C ++ +
Sbjct: 556 NPCKLRADPGPCKAVFIRYYYNWDWKECMKFTYGGCEGNANNFESIAQCEATCADKK-KKDNPCDLPAEPGPCRASFIRYYYNKQSKKCETFVYGGCEGNENSFETLEDCEARCIEHKKTEKNP---------------CDLPADAGPCKASIPRYYYNKNSKKCDTFVYGGCHGNENNFGSLEECQAKCAEQKKQENPCMLPADSGPCKAS---FTKYYYDWDLKKCVTFTYGGCQGN---ANNFESAAECEATC-AERKNSDKGP-------CELPADTGPCDGSLIRFYYNKDAGKCKTFKYGGCLGNQNNFESLQNCEAKCA-------GQKKTQQSPCELPAETGPCMASFIRFYYNKASKKCETFTYGGCQGNENNFESLQECEAKCAEQKKE 917 HSP 2 Score: 227.254 bits (578), Expect = 3.756e-59 Identity = 170/600 (28.33%), Postives = 260/600 (43.33%), Query Frame = 0
Query: 37 RYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEE--ILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTI---ATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC----EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
R+++++ S C+TF++ G N NNF + CE C++ N EE LR +P F + + +WD + +Y N S +CA + K +P + + PG SFI QS + T + + + + +PC L GPC + R++YN ++KC+ FV+GGC GN NNF SLEEC+ C+E K ++++PC+ P G C S K+++D+ C F Y GC GN NNF+S AEC++TC + + C P +G C + +N+ +C + Y GC G+ N F S + C+ +C + Q +P C + PC+ S + Y++ + C GC + N F + ECE C + E P C LR + G C R+YY + C FT+ GC GN NNF+S C ATC D + + + C+ P GPC R++YN + CE F YGGC+G+ N+F T CEA C+ ++
Sbjct: 512 RFYYDKQSKQCKTFTYGGRQGNANNFESLQECEARCVDQNKKEENPCKLRADPGP----CKAVFIRYYYNWDWKECMKFTYGGCEGNANNFESIAQCEATCADKKK-----KDNP----------------------CDLPAEPGPCRASFIRY-----YYNKQSKKCETFVYGGCEGNENSFETLEDCEARCIEHKKTEKNPCDLPADAGPCKASIPRYYYNKNSKKCDTFVYGGCHGNENNFGSLEECQAKCAEQK-KQENPCMLPADSGPCKASFTKYYYDWDLKKCVTFTYGGCQGNANNFESAAECEATCAERKN---------------SDKGPCELPADTGPCDGSLIRFYYNKDAGKCKTFKYGGCLGNQNNFESLQNCEAKCAGQKKTQQSP-CELPAETGPCMAS---FIRFYYNKASKKCETFTYGGCQGNE---NNFESLQECEAKCAEQKK---EENP------CKLRADAGPCKAIFVRYYYNWDWKECLKFTYGGCEGNANNFESIAQCEATCADKKKKDN--------PCDLPAEPGPCMASFIRYYYNKESKKCETFTYGGCEGNENSFETLKDCEARCVKQK 1035 HSP 3 Score: 219.935 bits (559), Expect = 1.208e-56 Identity = 167/609 (27.42%), Postives = 257/609 (42.20%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPR--CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C L E G C RY + C F++ GC N NNF CE C G ++E + P + + F + + D + + +Y N S C + K +P ++ + + ++ W + ++ + + S + + +PC L GPC IR++YN +++KCE FV+GGC+GN N+F++LE+CE C EHK + +PC P G C S +++++ S C FVY GC GN NNF SL ECQ+ C + K+ + C+ P SG C+ Y ++ +CV + Y GC G+ N F S C+ C E + + + C + PC S + Y+++ C GC + N F + CE C + + P C L E G C S RFYY + C +FT+ GC GN NNF+S Q C A C + + + L + GPC+ R++YN + C +F YGGC+G+ NNF + AQCEATC +++
Sbjct: 437 CKLPAETGPCKASLTRYHYAWDLKKCVAFTYGGCKGNSNNFESIAECEEKC--GKKEKDENPCELPAETGPRMA-SFIRFYYDKQSKQCKTFTYGGRQGNANNFESLQECEARCV---------DQNKKEENPCKLRADPGPCKAVFIRYYYNWDW-------------------KECMKFTYGGCEGNANNFESIAQCEATCADKKK-KDNPCDLPAEPGPCRASFIRYYYNKQSKKCETFVYGGCEGNENSFETLEDCEARCIEHKKTEKNPCDLPADAGPCKASIPRYYYNKNSKKCDTFVYGGCHGNENNFGSLEECQAKCAEQ----------------KKQENPCMLPADSGPCKASFTKYYYDWDLKKCVTFTYGGCQGNANNFESAAECEATCAERKNSDKGPCELPADTGPCDGS---LIRFYYNKDAGKCKTFKYGGCLGNQ---NNFESLQNCEAKCAGQK--KTQQSP------CELPAETGPCMASFIRFYYNKASKKCETFTYGGCQGNENNFESLQECEAKCAEQKKEENPCKLRADA--------GPCKAIFVRYYYNWDWKECLKFTYGGCEGNANNFESIAQCEATCADKK 975 HSP 4 Score: 216.468 bits (550), Expect = 1.989e-55 Identity = 138/442 (31.22%), Postives = 211/442 (47.74%), Query Frame = 0
Query: 220 ASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ---IAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHS------------------LEVEPMFAL-----------EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
+ST SS S A CQL +GPC+ ++R++Y+++T+ C F + GC GN NNF++ E+C+ C K ++PC P KG C K+ ++ +H C +FVY GC GN NNF + +C+ C T S+ +R L+ C P G C+ Y +N+ QC +IY GC G+ N F + CK RC I C + C + +W +S ++ +C + GC N + T ECER C++ + ++V + L C L + G+C GS PR+YY+ + CH FT+ GC GN NN+ S + C ATC G + CE P ++G C+ F+YN + C++F YGGC+G+ N F T+ QCEA C+ ++ +
Sbjct: 21 SSTQESSWS--ANDVCQLPAEQGPCEHQVMRWYYDFQTDTCMPFSYSGCGGNKNNFQTYEDCKAKCIVSKVLNENPCEQPKDKGRCFAVINKWFYNKTTHQCQQFVYGGCEGNQNNFKTKQQCEERC------TKQSI--------RRPLNPCELPAEVGPCKATLTRYYYNKASKQCETFIYGGCEGNKNNFGTKAECKKRCWADSALIVDGCNVPKDPGTCTKN---LYMWYFSRKRLVCEPFIFGGCGGN---ANRYVTKEECERRCVAPLPATAICSLPNAAGSCRRSLTRMKVTAILLLLFGATITLQKENPCNLPADTGACKGSFPRYYYDWNSKQCHQFTYGGCQGNANNYASLEECKATC-------PGEEQNDEDPCELPAVRGRCKAYIVVFYYNKESKKCDKFAYGGCEGNGNRFTTQEQCEAKCVGQKKE 433 HSP 5 Score: 209.149 bits (531), Expect = 5.918e-53 Identity = 129/395 (32.66%), Postives = 189/395 (47.85%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPC-LPS---PSYTS-VWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
+PC L G C R++Y+W +++C F +GGC+GN NN+ SLEEC+ TC + +DPC P ++G C F+++ +S C KF Y GC GNGN F + +C++ C +G+ K+ + C P +G C+ Y + +CV + Y GC G+ N F S C+ +C + E PC LP+ P S + Y ++Q T + N F + ECE C+ + E P C LR + G C R+YY + C FT+ GC GN NNF+S C ATC D + + + C+ P GPCR R++YN + CE F YGGC+G+ N+F T CEA C+
Sbjct: 314 NPCNLPADTGACKGSFPRYYYDWNSKQCHQFTYGGCQGNANNYASLEECKATCPGEEQNDEDPCELPAVRGRCKAYIVVFYYNKESKKCDKFAYGGCEGNGNRFTTQEQCEAKC----------VGQ------KKEENPCKLPAETGPCKASLTRYHYAWDLKKCVAFTYGGCKGNSNNFESIAECEEKCG-------KKEKDENPCELPAETGPRMASFIRFYYDKQSKQCKTFTYGGRQGNANNFESLQECEARCVD--QNKKEENP------CKLRADPGPCKAVFIRYYYNWDWKECMKFTYGGCEGNANNFESIAQCEATCADKKKKDN--------PCDLPAEPGPCRASFIRYYYNKQSKKCETFVYGGCEGNENSFETLEDCEARCI 669 HSP 6 Score: 182.57 bits (462), Expect = 3.256e-44 Identity = 131/445 (29.44%), Postives = 192/445 (43.15%), Query Frame = 0
Query: 226 STSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP-------------------------------------EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC----KDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR--CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
+ S L C + G C L ++++ K CE F+FGGC GN N + + EECE C P +K++PC P G+C GS ++++D+ S C +F Y GC GN NN+ SL EC++TC ++ DP C P G C+ V+ +N+ +C + Y GC G+ N F + E C+ +C Q C++ PC S + + Y+ + + CV GC K N F + +ECE C C L E G S RFYY+ + C +FT+ G GN NNF+S Q C A C D + + A GPC+ R++YN + C +F YGGC+G+ NNF + AQCEATC +++
Sbjct: 209 ADSALIVDGCNVPKDPGTCTKNLYMWYFSRKRLVCEPFIFGGCGGNANRYVTKEECERRCVAPLPATAICSLPNAAGSCRRSLTRMKVTAILLLLFGATITLQKENPCNLPADTGACKGSFPRYYYDWNSKQCHQFTYGGCQGNANNYASLEECKATCPGEEQNDEDP-------------------CELPAVRGRCKAYIVVFYYNKESKKCDKFAYGGCEGNGNRFTTQEQCEAKCVGQKKEENPCKLPAETGPCKAS---LTRYHYAWDLKKCVAFTYGGC---KGNSNNFESIAECEEKCGKKEKDE---------NPCELPAETGPRMASFIRFYYDKQSKQCKTFTYGGRQGNANNFESLQECEARCVDQNKKEENPCKLRAD-------PGPCKAVFIRYYYNWDWKECMKFTYGGCEGNANNFESIAQCEATCADKK 612 HSP 7 Score: 102.834 bits (255), Expect = 1.021e-18 Identity = 48/116 (41.38%), Postives = 74/116 (63.79%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEK--DDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
+PC L GPC IR++YN +++KCE F +GGC+GN N+F++L++CE C + K K + PC P G C S +++++ S C +F Y GC GN NNF+S+ +C++TC
Sbjct: 979 NPCDLPAEPGPCMASFIRYYYNKESKKCETFTYGGCEGNENSFETLKDCEARCVKQKKRKREESPCKLPADIGPCKMSIVRYYYNNGSKKCEQFTYGGCHGNENNFESMEKCEATC 1094 HSP 8 Score: 89.3521 bits (220), Expect = 1.423e-14 Identity = 67/264 (25.38%), Postives = 107/264 (40.53%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC 285
C L E G C R+++N++S CETF++ GC NENNF + CE C E + K + + + + +WD + +Y N S +CA + K +P + + PG SFI + ++ + + A + + SPC+L GPC + ++R++YN ++KCE F +GGC GN NNF+S+E+CE TC
Sbjct: 861 CELPAETGPCMASFIRFYYNKASKKCETFTYGGCQGNENNFESLQECEAKCAEQKKEENPCKLRADAGPCKAIFVRY---YYNWDWKECLKFTYGGCEGNANNFESIAQCEATCADKKK-----KDNP----------------------CDLPAEPGPCMASFIRYYYNKESKKCETFTYGGCEGNENSFETLKDCEARCVKQKKRKREESPCKLPADIGPCKMSIVRYYYNNGSKKCEQFTYGGCHGNENNFESMEKCEATC 1094
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|22901764|gb|AAN10061.1| (Kunitz-like protease inhibitor precursor [Ancylostoma caninum]) HSP 1 Score: 231.491 bits (589), Expect = 5.452e-62 Identity = 138/405 (34.07%), Postives = 194/405 (47.90%), Query Frame = 0
Query: 222 TLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKD-DPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEP--CLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
T + E C + GPC L R+ Y+ K KC F++GGCKGN NNF+S+EEC TC + PE + D C P+ G C +++ +D + + C +F+Y GC GN NNF+S+ EC TCK + P+ C +PI G C+ Y ++ +N+CV +IY GC G+ N F S E C C+ + P + +P P + + Y ++ CV + GC K N F + EC RTC EP E C E G C + RF Y+ E C F + GC GN NNF++ + C TC + + +C QP GPCR PR+ Y+ C +F YGGCKG+ N FPT+ +CE TC
Sbjct: 254 TCKKAVPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAVPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAVPE------------PEPEKETCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAV-PEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNK---NNFESMEECTRTC-----KKAVPEPEPEKETCSQPIEAGPCKAMVRRFAYDNAKEKCVEFFYGGCKGNKNNFETMEDCTFTCEQRLAKPE----LEKDVCSQPITAGPCRASIPRYGYDSKKRKCVKFTYGGCKGNGNRFPTKNECEKTC 633 HSP 2 Score: 226.098 bits (575), Expect = 4.709e-60 Identity = 183/623 (29.37%), Postives = 266/623 (42.70%), Query Frame = 0
Query: 13 SVEHVIPEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMN--GNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTN--QSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKD---DPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIA-PRCRIEHTFEPCLPSPSYTSV--WVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
S E +P+ CLL E G C ERR++F+Q G C++F F GCG N NNF K C C TE+++ Q P ++ + + +++ Q I Y N S + +R+C K+ P P Q ++ I + +L+ ++ ++N + N +S+ T T + E C + GPC L R+ Y+ K KC F++GGCKGN NNF+S+EEC TC + PE + + C P+ G C +++ +D + + C +F+Y GC GN NNF+S+ EC TCK VP+ C +PI G C+ Y ++ +N+CV +IY GC G+ N F S E C C+ + P E +P P V + Y + CV GC K N F T +C TC +P + C G C S+PR+ Y+ C FT+ GC GNGN F + C TC N C P + GPC+ R+ YN C F YGGC G+ NNF T+ C+ C N
Sbjct: 133 SEEESVPDACLLPSEVGPCKGKERRFYFDQKRGNCKSFFFGGCGGNGNNFMTKAKCMETCSKHIKPETEQDVCSQ-PIKAGPCMAMLKRYAYDNKKKRCVQFI-YGGCKGNKNNFESMEECTRTC---------KKAVP---EPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECT----------------RTCKKAVPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAVPEPEPEKETCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKA--------------VPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAVPEPEPEKETCSQPIEAGPCKAMVRRFAYDNAKEKCVEFFYGGCKGNK---NNFETMEDCTFTC-----EQRLAKPELEKDVCSQPITAGPCRASIPRYGYDSKKRKCVKFTYGGCKGNGNRFPTKNECEKTCKRGATGTTNPGGENDK-CLLPIVTGPCKGKNRRYAYNNKTGKCVRFTYGGCGGNENNFKTKKDCQDACEN 702 HSP 3 Score: 223.787 bits (569), Expect = 3.780e-59 Identity = 138/396 (34.85%), Postives = 191/396 (48.23%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKD-DPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIA-PRCRIEHTFEP--CLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNR 624
C + GPC L R+ Y+ K ++C F++GGCKGN NNF+S+EEC TC + PE + D C P+ G C +++ +D + + C +F+Y GC GN NNF+S+ EC TCK VP+ C +PI G C+ Y ++ +N+CV +IY GC G+ N F S E C C+ + P E +P P + + Y ++ CV + GC K N F + EC RTC + P + C E G C L R+ Y+ C F + GC GN NNF+S + C TC A + T C QP GPC+ RF Y+ + E C EF YGGCKG++NNF T C TC R
Sbjct: 205 CSQPIKAGPCMAMLKRYAYDNKKKRCVQFIYGGCKGNKNNFESMEECTRTCKKAVPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKA--------------VPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAVPEPEPEKETCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNK---NNFESMEECTRTCK-------KAVPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAVPEPEPEKET----CSQPIEAGPCKAMVRRFAYDNAKEKCVEFFYGGCKGNKNNFETMEDCTFTCEQR 572 HSP 4 Score: 219.935 bits (559), Expect = 7.062e-58 Identity = 171/617 (27.71%), Postives = 265/617 (42.95%), Query Frame = 0
Query: 15 EHVIPE----FCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKD-DPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIA-PRCRIEHTFEPCLPSPSYTSV--WVYSEEQRMCV-VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
+H+ PE C + G C +RY ++ C F + GC N+NNF + C R C E+ +P + +++ N + I Y N S + +R+C K+ P P Q ++ I + +L+ ++ ++N + N + T + +P P S E+ GPC L R+ Y+ K KC F++GGCKGN NNF+S+EEC TC + PE + D C P+ G C +++ +D + + C +F+Y GC GN NNF+S+ EC TCK + P+ C +PI +G C+ + ++ + +CV + Y GC G+ N F + E C CE ++A P + +P P S+ + Y ++R CV T G K GN F T +ECE+TC R + P + CLL G C G R+ Y C FT+ GC GN NNF++ + C C + + AS C P KG + R+ + C F+YGG +G+ NNF ++A C+ CL
Sbjct: 194 KHIKPETEQDVCSQPIKAGPCMAMLKRYAYDNKKKRCVQFIYGGCKGNKNNFESMEECTRTCKKAVPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFI-YGGCKGNKNNFESMEECTRTC---------KKAVP---EPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKN-NFESMEECTRTCKKAVPEPEPEKETCSQPIEV-----------GPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAVPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAVPE------------PEPEKETCSQPIEAGPCKAMVRRFAYDNAKEKCVEFFYGGCKGNKNNFETMEDCTFTCEQRLAKPELEKDVCSQPITAGPCRASIPRYGYDSKKRKCVKFTYG--GCKGNGNRFPTKNECEKTC--KRGATGTTNPGGENDKCLLPIVTGPCKGKNRRYAYNNKTGKCVRFTYGGCGGNENNFKTKKDCQDACEN---------INAASPCTLPIDKGEGDLNLTRYGFKNG--KCVAFKYGGRRGNLNNFGSKADCKEACL 758 HSP 5 Score: 186.037 bits (471), Expect = 5.874e-46 Identity = 135/454 (29.74%), Postives = 192/454 (42.29%), Query Frame = 0
Query: 223 LSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD----------------HMDPTMNSLGRHIFR-----------------------------------VPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEP--CLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
L ++T+ C+ + G C +F Y+ T KC + +GGC G+ N F +LEEC+ C KPE D C PL +G C S +++ +D S C F+Y GCGGN NNF+++AEC+ TCKD + P R F P+ C +PI +G C Y ++ + +CV +IY GC G+ N F S E C C+ + P + +P P + + Y ++ CV + GC K N F + EC RTC + P + C E G C L R+ Y+ C F + GC GN NNF+S + C TC A + T C QP GPC+ R+ Y+ C F YGGCKG++NNF + +C TC
Sbjct: 9 LWAATATALLDICKEEIKTGNCRGAFRKFGYDRCTNKCIPYTYGGCGGSSNMFDTLEECQEKCG--KPE--DRCSKPLERGICLASMKRYGYDTSSKKCKAFIYGGCGGNENNFETMAECRETCKDTSSEEESVPDACLLPSEVGPCKGKERRFYFDQKRGNCKSFFFGGCGGNGNNFMTKAKCMETCSKHIKPETEQDVCSQPIKAGPCMAMLKRYAYDNKKKRCVQFIYGGCKGNKNNFESMEECTRTCKKAV-PEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNK---NNFESMEECTRTC-------KKAVPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAVPEPEPEKET----CSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTC 443 HSP 6 Score: 182.57 bits (462), Expect = 9.273e-45 Identity = 137/404 (33.91%), Postives = 189/404 (46.78%), Query Frame = 0
Query: 224 SSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH-KPEKD-DPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEP--CLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
+SS E C L GPC RF+++ K C++F FGGC GNGNNF + +C TCS+H KPE + D C P+ G C +++ +D + C +F+Y GC GN NNF+S+ EC TCK VP+ C +PI G C+ Y ++ +N+CV +IY GC G+ N F S E C C+ + P + +P P + + Y ++ CV + GC K N F + EC RTC EP E C E G C L R+ Y+ C F + GC GN NNF+S + C TC A + C QP GPC+ R+ Y+ C F YGGCKG++NNF + +C TC
Sbjct: 131 TSSEEESVPDACLLPSEVGPCKGKERRFYFDQKRGNCKSFFFGGCGGNGNNFMTKAKCMETCSKHIKPETEQDVCSQPIKAGPCMAMLKRYAYDNKKKRCVQFIYGGCKGNKNNFESMEECTRTCKKA--------------VPEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAV-PEPEQDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNK---NNFESMEECTRTC-----KKAVPEPEPEKETCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTCKKAVPEPE------QDTCSQPIEVGPCKAMLKRYAYDNKKNKCVRFIYGGCKGNKNNFESMEECTRTC 505
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|669328878|gb|KFD69065.1| (hypothetical protein M514_18642 [Trichuris suis]) HSP 1 Score: 230.335 bits (586), Expect = 1.040e-60 Identity = 179/660 (27.12%), Postives = 280/660 (42.42%), Query Frame = 0
Query: 15 EHVIPEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLL------RIESW-----PGRRNNSFIVMNDLVVLQTNQSLRPSTIATDS--TTTTLP---------------------STTPASTLSSSTSELAG-------SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR--CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG----CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
EH C L + G C Y +Y++N+ S CETF + GC NEN F + CE MC+ EEE P S F + + DWD +Y N S +C G + PK P ++ + + K + E++ G ++ + D + + P + DS P + ++L + G PC+L GPC Y +++YN +++KCE FV+GGC+GN N F +LEECE C+E K E ++PC P G C GS ++++D+ C +F Y GC GN NN+ SL EC++TC + + + S C +G C Y +N+ +C ++Y GCGG++N F + E C+ +C Q C++ PC S + + Y + + C GC K N +++ EC+ TC ++ E C L + G C ++YY + C +F + GC GN N F + + C A C AE + + + C+ P GPC+ R++Y+ ++ C EF YGGCKG+ NN+ + +C+ATC E
Sbjct: 77 EHKEQNPCELPADSGPCMAYFTKYYYNKESKKCETFVYGGCAGNENRFDTLEDCEAMCIE----EEENPCILPADSGP-CKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPG--------EEEPKDEDPCELPADSGPCMAYFTKYYYNKESKKCETFVYGGCQGNEADAQAMKIDCEAVCIEEEENPCILPADSGPCKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPGEEEPKDEDPCELPADSGPCLAYFTKYYYNKESKKCETFVYGGCQGNDNRFDTLEECEAKCAEQKRE-ENPCKLPADSGPCRGSFPRYYYDWDLKQCREFTYGGCKGNANNYASLQECEATCPGEK---HKEEPKEEPKEEPKVESPCELQSDTGPCMAYFTKYYYNKESRKCETFVYGGCGGNENRFDTLEECEAKCAEQKKEENPCKLPADTGPCKAS---FTRYYYDWDLKQCREFTYGGC---KGNANNYASLQECQATCPTEEHKEEPKEEPKEEPKVENPCELPSDTGPCMAYFTKYYYNKESKKCETFVYGGCGGNENRFDTLEECEAKC--AEQKKE------ENPCKLPADTGPCKASFTRYYYDWDLKQCREFTYGGCKGNANNYASLQECQATCPGEE 705 HSP 2 Score: 216.853 bits (551), Expect = 4.863e-56 Identity = 128/402 (31.84%), Postives = 199/402 (49.50%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR----CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSS---ECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
+PC+L GPC R++Y+W ++C F +GGC+GN NN+ S++ECE TCS+ + ++ +PC P G C K++++ +S C FVY GC GN N FD+L +C++ C + + + C+ P SG C+ + Y ++ +CV + Y GC G+ N + S + C+ C + P+ C + PC+ +Y + + Y++E + C V GC GNE + +CE C+ + + C+L + G C G PR+YY+ + C FT+ GC GN NN+ S Q C ATC E D CE P GPC +++YN + CE F YGGC+G+ N F T +CEA C ++
Sbjct: 21 NPCKLPAETGPCRGSFTRYYYDWDLKQCLEFTYGGCEGNANNYDSVQECEATCSDGEHKEQNPCELPADSGPCMAYFTKYYYNKESKKCETFVYGGCAGNENRFDTLEDCEAMCIEEEE------------------NPCILPADSGPCKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPGEEEPKDEDPCELPADSGPCM---AYFTKYYYNKESKKCETFVYGGCQ-----GNEADAQAMKIDCEAVCIEEEENP-----------CILPADSGPCKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPGEEEPKD------EDPCELPADSGPCLAYFTKYYYNKESKKCETFVYGGCQGNDNRFDTLEECEAKCAEQK 379 HSP 3 Score: 211.846 bits (538), Expect = 2.940e-54 Identity = 164/618 (26.54%), Postives = 261/618 (42.23%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPT--MNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR--CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDR--------SHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAE---VRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
C+L + G C RY+++ C F++ GC N NN+ + CE C ++E + P S L F + + + ++ + Y + + CA R + K P P + LK R ++ G + N+ N+ LQ ++ P + SPC+L GPC Y +++YN ++ KCE FV+GGC GN N F +LEECE C+E K E ++PC P G C S ++++D+ C +F Y GC GN NN+ SL ECQ+TC PT + + + + C P +G C Y +N+ +C ++Y GCGG++N F + E C+ +C Q C++ PC S + + Y + + C GC K N +++ EC+ TC + E C L + G C ++YY + C +F + GC GN N F + + C A C + + V G C PK GPC+ +++++ + C F YGGCKG+ N F T+ +C+ C+
Sbjct: 263 CILPADSGPCKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPGEEEPKDEDPCELPADSGPCLAY-FTKYYYNKESKKCETFVYGGCQGNDNRFDTLEECEAKCAEQKREENPCK-LPADSGPCRGSFPR-YYYDWDLKQCREFTYGGCKGNA----NNYASLQECEATCPGEKHKEEPKE-----------EPKEEPKVESPCELQSDTGPCMAYFTKYYYNKESRKCETFVYGGCGGNENRFDTLEECEAKCAEQKKE-ENPCKLPADTGPCKASFTRYYYDWDLKQCREFTYGGCKGNANNYASLQECQATC-----PTEEHKEEPKEEPKEEPKVENPCELPSDTGPCMAYFTKYYYNKESKKCETFVYGGCGGNENRFDTLEECEAKCAEQKKEENPCKLPADTGPCKAS---FTRYYYDWDLKQCREFTYGGC---KGNANNYASLQECQATCPGEEHKEEPKQEPKEEPKEEPKVENPCELPSDSGPCMAYFTKYYYNKESKKCETFVYGGCQGNENRFDTLEECEAKCGEDKKEGVPPAGPDAPTDDPCILPKDSGPCKASHRKWYFDTHRKVCRMFVYGGCKGNANRFDTKEECQQRCV 850 HSP 4 Score: 211.46 bits (537), Expect = 3.698e-54 Identity = 173/639 (27.07%), Postives = 259/639 (40.53%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKA-CERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC------------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR---------------CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAE---------VRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C L + G C Y +Y++N+ S CETF + GC NE + + K CE +C+ EEE P S F + + DWD +Y N S +C G + PK P ++ + + K + ++ +F+ D+ TL + + +PC+L GPC R++Y+W ++C F +GGCKGN NN+ SL+ECE TC + +P+ + PC G C K++++ +S C FVY GCGGN N FD+L EC++ C + K+ + C P +G C+ Y ++ QC + Y GC G+ N + S + C+ C + C + PC+ +Y + + Y++E + C V GC + N F T ECE C + E P C L + G C S R+YY+ + C FT+ GC GN NN+ S Q C ATC E + CE P GPC +++YN + CE F YGGC+G+ N F T +CEA C
Sbjct: 204 CELPADSGPCMAYFTKYYYNKESKKCETFVYGGCQGNEADAQAMKIDCEAVCIE----EEENPCILPADSGP-CKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPG--------EEEPKDEDPCELPADSGPCLAYFTKYYYNKE--SKKCETFVYG--------------GCQGNDNRFDTLEECE----AKCAEQKREENPCKLPADSGPCRGSFPRYYYDWDLKQCREFTYGGCKGNANNYASLQECEATCPGEKHKEEPKEEPKEEPKVESPCELQSDTGPCMAYFTKYYYNKESRKCETFVYGGCGGNENRFDTLEECEAKCAEQ----------------KKEENPCKLPADTGPCKASFTRYYYDWDLKQCREFTYGGCKGNANNYASLQECQATCPTEEHKEEPKEEPKEEPKVENPCELPSDTGPCM---AYFTKYYYNKESKKCETFVYGGCGGNE---NRFDTLEECEAKCAEQKK---EENP------CKLPADTGPCKASFTRYYYDWDLKQCREFTYGGCKGNANNYASLQECQATCPGEEHKEEPKQEPKEEPKEEPKVENPCELPSDSGPCMAYFTKYYYNKESKKCETFVYGGCQGNENRFDTLEECEAKC 778 HSP 5 Score: 181.8 bits (460), Expect = 4.356e-44 Identity = 121/426 (28.40%), Postives = 189/426 (44.37%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC------------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ-------------------IAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFAL--EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
+PC+L GPC R++Y+W ++C F +GGCKGN NN+ SL+EC+ TC + +P+ ++PC P G C K++++ +S C FVY GCGGN N FD+L EC++ C + K+ + C P +G C+ Y ++ QC + Y GC G+ N + S + C+ C + + C + PC+ +Y + + Y++E + C V GC + N F T ECE C D+ + A + C+L + G C S ++Y++ + C F + GC GN N F + + C C + L + IC P G C RFFY+ + C F Y GC+G+ NNF + C C +
Sbjct: 516 NPCKLPADTGPCKASFTRYYYDWDLKQCREFTYGGCKGNANNYASLQECQATCPTEEHKEEPKEEPKEEPKVENPCELPSDTGPCMAYFTKYYYNKESKKCETFVYGGCGGNENRFDTLEECEAKCAEQ----------------KKEENPCKLPADTGPCKASFTRYYYDWDLKQCREFTYGGCKGNANNYASLQECQATCPGEEHKEEPKQEPKEEPKEEPKVENPCELPSDSGPCM---AYFTKYYYNKESKKCETFVYGGCQGNE---NRFDTLEECEAKCGEDKKEGVPPAGPDAPTDDPCILPKDSGPCKASHRKWYFDTHRKVCRMFVYGGCKGNANRFDTKEECQQRCV--------ATLPASVICRLPYAMGSCSSKIRRFFYDFETKQCRAFTYTGCEGNANNFISAHACYEKCAS 911
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|674589112|emb|CDS31835.1| (Papilin [Hymenolepis microstoma]) HSP 1 Score: 226.098 bits (575), Expect = 4.715e-59 Identity = 180/646 (27.86%), Postives = 279/646 (43.19%), Query Frame = 0
Query: 19 PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRS---LKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGR---RNNSFIVMNDLVV------LQTNQSLRPSTIATDSTTTTLPSTT---PASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMD-PTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC--------------------EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG-CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSF----QHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
P+FC+L + G C+ RYF+++ C F F GCG N NNF + CE+ C + + + S L + T + + W ++S L R + G K + PIQ D+ + L +S P R R S+ +L V Q N + + A ++ T PS PA + S++ + C L GPC L R+ ++ +C F +GGC+GN NNF++L++CE C + C PL G C G +K+ FD S C +FVY GCGGN NNF++ C+ C+ + P N+ ++ + L D G C+ + F+ + +C ++Y GC G+ N F + EAC+ +C +Q P C++ H C+ +Y W ++ + CV + GC + N F T ECE+ C ++ L +++ C L E G C S+ R+ ++ C T+ GC GN NNF+S Q CL + + +L + +C P+ GPCR R+ Y+ S +C EF YGGC+G+ NNF TR CE C
Sbjct: 132 PDFCMLEKDPGPCYAALPRYFYDKRLDKCTQFIFGGCGGNRNNFESIELCEKQCKDPKGDSDPVSENPGDTSVCLLPHETGNCRGYIKRW--------GFDSSLGRCVQFIYGGCGGNGNNFESLEACEQKCLAHIPMPIQTDKLSEMDPVCKLAQ---DSGPCRGSIRRWSYDATRNLCVEFIYGGCQGNSNNFETREACEAKCLTNPSRNVIPPALSGGISSNPI----CMLPQDSGPCRAQLPRWAFDASKGQCVQFTYGGCRGNENNFETLKQCEDACGRSEIMDTSQCHLPLEPGRCRGFFKKWGFDPSSGQCIQFVYGGCGGNSNNFETKEACEQQCRASIPMPLGNNDNLNVADPVCKLLKD------PGPCRGSIRRWAFDSQKGECTEFVYGGCQGNGNNFETREACEAKCLLSSSPAVQPISNVDHVVRNNVQQIPICQLPHERGECI---AYIPRWGFNAQLGKCVQFIYGGCGGNE---NNFETQEECEKRCNANAPMPLPGASSNSIDPICKLPKEVGPCRASIRRWAFDYSKGICEELTYGGCRGNANNFESKEACEQKCLLSSSPQTSATNNDVLDD--VCRLPQDTGPCRSSIKRWSYDVSKGSCVEFIYGGCRGNDNNFETREACEQRC 748 HSP 2 Score: 218.394 bits (555), Expect = 2.318e-56 Identity = 141/423 (33.33%), Postives = 193/423 (45.63%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP---------EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD-CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCE-------------------IQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFAL-----EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
CQL RG C Y+ R+ +N + KC F++GGC GN NNF++ EECE C+ + P D C P G C S ++ FD+ C + Y GC GN NNF+S C+ C P ++ + L D C P +G C+ + ++ ++ CV +IY GC G+DN F + EAC+ RC+ + C + PC S W Y+ Q CV + GC N F + CE C + S EP L + C L ERG+C+G R+ +EP C F + GC GN NNF+S + C C + + T IC PK GPCR Q R+ Y+ S TC EF YGGC+G+RNNF +R CEA C
Sbjct: 558 CQLPHERGECIAYIPRWGFNAQLGKCVQFIYGGCGGNENNFETQEECEKRCNANAPMPLPGASSNSIDPICKLPKEVGPCRASIRRWAFDYSKGICEELTYGGCRGNANNFESKEACEQKCLLSSSPQTSATNNDV-------LDDVCRLPQDTGPCRSSIKRWSYDVSKGSCVEFIYGGCRGNDNNFETREACEQRCQPSSSSNLAEVPATLDLDENSNLDQVCSLPAVVGPC---KSSYRRWAYNALQGGCVEFIYGGCGGN---ANNFESKEACEARCGQNDGSSTGSEPKIPLIMGDNDICFLPQERGNCYGFFERWGFEPKTGQCTMFIYGGCGGNENNFESKEACELRCIPGAPQTPKNDGTKDPICLLPKDVGPCRASQRRWAYDTSTNTCVEFIYGGCRGNRNNFESREACEAKC 967 HSP 3 Score: 207.223 bits (526), Expect = 1.322e-52 Identity = 141/426 (33.10%), Postives = 209/426 (49.06%), Query Frame = 0
Query: 220 ASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD---HMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIA------------PRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG-------CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
A+ ST E+ C L A GPC + R+FY+ + C++F++GGC GNGNNF++ ++C TCS K D C+ G C + ++ +D + C++F++ GCGGN NNF+S+ C+ CKD DP + G S CL P +G+C+ +GF+ + +CV +IY GCGG+ N F S EAC+ +C I P C++ PC S W Y + +CV + GC N F T CE CL++ S ++ + P AL G C+L + G C LPR+ ++ C FT+ GC GN NNF++ + C C +E+ + S C P G CR ++ ++PS C +F YGGC G+ NNF T+ CE C
Sbjct: 63 AAASGYSTPEI----CILPAASGPCKANIKRYFYDSTSGVCKDFIYGGCSGNGNNFETFDDCMRTCSSKPVNKPDFCMLEKDPGPCYAALPRYFYDKRLDKCTQFIFGGCGGNRNNFESIELCEKQCKDPKGDSDPVSENPG---------DTSVCLLPHETGNCRGYIKRWGFDSSLGRCVQFIYGGCGGNGNNFESLEACEQKCLAHIPMPIQTDKLSEMDPVCKLAQDSGPCRGS---IRRWSYDATRNLCVEFIYGGCQGNS---NNFETREACEAKCLTNPSRNV-IPP--ALSGGISSNPICMLPQDSGPCRAQLPRWAFDASKGQCVQFTYGGCRGNENNFETLKQCEDACGRSEI-------MDTSQCHLPLEPGRCRGFFKKWGFDPSSGQCIQFVYGGCGGNSNNFETKEACEQQC 459 HSP 4 Score: 201.83 bits (512), Expect = 1.084e-50 Identity = 135/426 (31.69%), Postives = 196/426 (46.01%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP---------EKDDPCISPLLK--GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGR--HIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ------------IAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG-CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHD------AEVRADGSILTNAS---ICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
S C L + G C + ++ ++ + +C FV+GGC GN NNF++ E CE C P DP + LLK G C GS ++ FD Q C++FVY GC GNGNNF++ C++ C P + + H+ R + + C P G+C +GFN +CV +IY GCGG++N F + E C+ RC I P C++ PC S W + + +C + GC N F + CE+ CL S L+ C L + G C S+ R+ Y+ +C F + GC GN NNF++ + C C AEV A + N++ +C P + GPC+ R+ YN C EF YGGC G+ NNF ++ CEA C
Sbjct: 407 SQCHLPLEPGRCRGFFKKWGFDPSSGQCIQFVYGGCGGNSNNFETKEACEQQCRASIPMPLGNNDNLNVADP-VCKLLKDPGPCRGSIRRWAFDSQKGECTEFVYGGCQGNGNNFETREACEAKCLLSSSPAVQPISNVDHVVRNNVQQIPICQLPHERGECIAYIPRWGFNAQLGKCVQFIYGGCGGNENNFETQEECEKRCNANAPMPLPGASSNSIDPICKLPKEVGPCRAS---IRRWAFDYSKGICEELTYGGCRGN---ANNFESKEACEQKCLLSSSPQTSATNNDVLDDVCRLPQDTGPCRSSIKRWSYDVSKGSCVEFIYGGCRGNDNNFETREACEQRCQPSSSSNLAEVPATLDLDENSNLDQVCSLPAVVGPCKSSYRRWAYNALQGGCVEFIYGGCGGNANNFESKEACEARC 825 HSP 5 Score: 192.2 bits (487), Expect = 1.610e-47 Identity = 132/428 (30.84%), Postives = 191/428 (44.63%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDP------------CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIA-------------PRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG-----------CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCH-DAEVRADGSILTNAS-ICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
C+LA GPC + R+ Y+ C F++GGC+GN NNF++ E CE C + P C+ P G C ++ FD C +F Y GC GN NNF++L +C+ C R S C P+ G C+ +GF+ + QC+ ++Y GCGG+ N F + EAC+ +C I P C++ PC S W + ++ C V GC GN F T CE CL S S V+P+ ++ C L ERG C +PR+ + C F + GC GN NNF++ + C C+ +A + G+ + IC+ PK GPCR R+ ++ S CEE YGGC+G+ NNF ++ CE CL
Sbjct: 275 CKLAQDSGPCRGSIRRWSYDATRNLCVEFIYGGCQGNSNNFETREACEAKCLTNPSRNVIPPALSGGISSNPICMLPQDSGPCRAQLPRWAFDASKGQCVQFTYGGCRGNENNFETLKQCEDACG---------------RSEIMDTSQCHLPLEPGRCRGFFKKWGFDPSSGQCIQFVYGGCGGNSNNFETKEACEQQCRASIPMPLGNNDNLNVADPVCKLLKDPGPCRGS---IRRWAFDSQKGECTEFVYGGCQGN---GNNFETREACEAKCL--LSSSPAVQPISNVDHVVRNNVQQIPICQLPHERGECIAYIPRWGFNAQLGKCVQFIYGGCGGNENNFETQEECEKRCNANAPMPLPGASSNSIDPICKLPKEVGPCRASIRRWAFDYSKGICEELTYGGCRGNANNFESKEACEQKCL 679 HSP 6 Score: 173.711 bits (439), Expect = 2.541e-41 Identity = 130/453 (28.70%), Postives = 192/453 (42.38%), Query Frame = 0
Query: 216 STTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDP-----------------CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMD-PTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR------------CRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSL-EVEPMFALEG-------CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGS------ILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
++ P ++ +A C+L GPC + R+ ++ + +C FV+GGC+GNGNNF++ E CE C P C P +G C ++ F+ Q C +F+Y GCGGN NNF++ EC+ C + P + I + C P G C+ + F+ ++ C Y GC G+ N F S EAC+ +C + +P+ CR+ PC S W Y + CV + GC N F T CE+ C S +L EV L+ C L G C S R+ Y + C F + GC GN NNF+S + C A C + + GS I+ + IC P+ +G C R+ + P C F YGGC G+ NNF ++ CE C+
Sbjct: 461 ASIPMPLGNNDNLNVADPVCKLLKDPGPCRGSIRRWAFDSQKGECTEFVYGGCQGNGNNFETREACEAKCLLSSSPAVQPISNVDHVVRNNVQQIPICQLPHERGECIAYIPRWGFNAQLGKCVQFIYGGCGGNENNFETQEECEKRCNANAPMPLPGASSNSIDPI-------CKLPKEVGPCRASIRRWAFDYSKGICEELTYGGCRGNANNFESKEACEQKCLLSSSPQTSATNNDVLDDVCRLPQDTGPCR---SSIKRWSYDVSKGSCVEFIYGGCRGND---NNFETREACEQRCQPSSSSNLAEVPATLDLDENSNLDQVCSLPAVVGPCKSSYRRWAYNALQGGCVEFIYGGCGGNANNFESKEACEARCGQNDGSSTGSEPKIPLIMGDNDICFLPQERGNCYGFFERWGFEPKTGQCTMFIYGGCGGNENNFESKEACELRCI 900 HSP 7 Score: 150.984 bits (380), Expect = 5.150e-34 Identity = 103/350 (29.43%), Postives = 154/350 (44.00%), Query Frame = 0
Query: 296 CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCE--------IQIAPR----CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG-CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVR------ADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREV 626
CI P G C + +++ +D S C F+Y GC GNGNNF++ +C TC P C+ G C Y +++ ++C +I+ GCGG+ N F S E C+ +C+ + P C + H C Y W + CV + GN F + CE+ CL+ ++ + + ++ C L + G C GS+ R+ Y+ C F + GC GN NNF++ + C A C R G I +N IC P+ GPCR PR+ ++ S C +F YGGC+G+ NNF T QCE C E+
Sbjct: 74 CILPAASGPCKANIKRYFYDSTSGVCKDFIYGGCSGNGNNFETFDDCMRTCSSK---------------PVNKPDFCMLEKDPGPCYAALPRYFYDKRLDKCTQFIFGGCGGNRNNFESIELCEKQCKDPKGDSDPVSENPGDTSVCLLPHETGNCR---GYIKRWGFDSSLGRCVQFIY-GGCGGNGNNFESLEACEQKCLAHIPMPIQTDKLSEMDPVCKLAQDSGPCRGSIRRWSYDATRNLCVEFIYGGCQGNSNNFETREACEAKCLTNPSRNVIPPALSGGISSNP-ICMLPQDSGPCRAQLPRWAFDASKGQCVQFTYGGCRGNENNFETLKQCEDACGRSEI 403 HSP 8 Score: 120.168 bits (300), Expect = 3.137e-24 Identity = 87/303 (28.71%), Postives = 132/303 (43.56%), Query Frame = 0
Query: 214 LPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCS----------------EHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-----------CRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSD 487
L S++P + S++ +++ C+L GPC + R+ Y+ C F++GGC+GN NNF++ E CE C + D C P + G C S ++ ++ C +F+Y GCGGN NNF+S C++ C + + S + + + C P G+C +GF QC +IY GCGG++N F S EAC+ RC I AP+ C + PC S W Y CV + GC + N F + CE C S+
Sbjct: 679 LLSSSPQT--SATNNDVLDDVCRLPQDTGPCRSSIKRWSYDVSKGSCVEFIYGGCRGNDNNFETREACEQRCQPSSSSNLAEVPATLDLDENSNLDQVCSLPAVVGPCKSSYRRWAYNALQGGCVEFIYGGCGGNANNFESKEACEARCGQNDGSSTGSEPKIPLIMGDNDI--CFLPQERGNCYGFFERWGFEPKTGQCTMFIYGGCGGNENNFESKEACELRC-IPGAPQTPKNDGTKDPICLLPKDVGPCRAS---QRRWAYDTSTNTCVEFIYGGCRGNR---NNFESREACEAKCGSN 970 HSP 9 Score: 93.2041 bits (230), Expect = 8.207e-16 Identity = 45/128 (35.16%), Postives = 66/128 (51.56%), Query Frame = 0
Query: 501 EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDA 628
E C+L G C ++ R++Y+ C F + GC GNGNNF++F C+ TC V C K GPC PR+FY+ ++ C +F +GGC G+RNNF + CE C + + D+
Sbjct: 72 EICILPAASGPCKANIKRYFYDSTSGVCKDFIYGGCSGNGNNFETFDDCMRTCSSKPV-------NKPDFCMLEKDPGPCYAALPRYFYDKRLDKCTQFIFGGCGGNRNNFESIELCEKQCKDPKGDS 192
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|730368910|gb|KHJ40845.1| (Kunitz/Bovine pancreatic trypsin inhibitor domain protein [Trichuris suis]) HSP 1 Score: 227.639 bits (579), Expect = 7.026e-59 Identity = 150/468 (32.05%), Postives = 213/468 (45.51%), Query Frame = 0
Query: 212 TTLPSTTPAST--LSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKR------SLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTS--VWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAE--------VRADGS--------ILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVV 651
+T P PA+T + C G C R++YN EKCE F++ GC GN NNF S+ C+ TC E D C P G+C+G ++++FD + C +YSGCGGNGNNFDS A C+ C + +NS + VP + L+ C P G C + + +++ E C P+ Y+GCGG+ N F S E C N C F P P S W Y + MC GC+ N F + + C C P CL +RG C G R++Y+ +TC F + GC G GNNF + Q CL C +AE ++A+ + IL C+QPK++G C + + R+F+N + CE F Y GC G+RNNF T +QCE C R + + ++++ F ES S V
Sbjct: 420 STAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLELD---ADICKHPPNVGTCTGRIQRWYFDRPARKCKVLLYSGCGGNGNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGNGNNFESREVCMNTCPNDDTSHLPPNPCFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGN---ANNFESFASCMVKC----------GPEILSSRCLHPTDRGDCSGHFVRYHYDSDSKTCKEFVYGGCGGTGNNFATKQECLRACANAEADDEVKKLLKAERTPVSIQPTLILPTEDSCKQPKMRGNCNKYELRYFFNAQVGRCEFFFYSGCGGNRNNFKTLSQCELFCSKRRPNPLLFGSSDDATAFSSESYGSTEAV 871 HSP 2 Score: 199.134 bits (505), Expect = 1.761e-49 Identity = 135/448 (30.13%), Postives = 196/448 (43.75%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC--------------------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSV---------------------WVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESET 639
C+ RGPC + +RFFY+ + +C F + GC GN NNFKSL +C+ C + P+ ++ C P GSC S ++++++ C F+YSGCGGN NNFDS+ CQ TC + +D + C P G C + F++ +C +YSGCGG+ N F S +C+ C +++ + +P +V W Y E + MC GC GN F + C TC +D + L P C R + G C S R++Y+ CH FT+ GC GN NNF+SF C+ C IL+ S C P +G C R+ Y+ +TC+EF YGGC G+ NNF T+ +C C N E D ++ L + T
Sbjct: 364 CRFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLSDCQKFCIRGLKSTAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLE-LDADI-----------------CKHPPNVGTCTGRIQRWYFDRPARKCKVLLYSGCGGNGNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGN---GNNFESREVCMNTCPNDDTSHLPPNP------CFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGNANNFESFASCMVKCGP-------EILS--SRCLHPTDRGDCSGHFVRYHYDSDSKTCKEFVYGGCGGTGNNFATKQECLRACANAEADDEVKKLLKAERT 775 HSP 3 Score: 177.948 bits (450), Expect = 1.526e-42 Identity = 113/351 (32.19%), Postives = 160/351 (45.58%), Query Frame = 0
Query: 291 EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHM-----DPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEP-------------MFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
E + C P +G C +F +D + C F YSGCGGN NNF SL++CQ C + D + R + P + + C P +G C+ + +N E +C P+IYSGCGG+ N F S C+ C A C+ C W + R C V + GC GN F + + CER CL + S + +P + L C GSC G R+YY+ ++E C FT++GC GNGNNF+S + C+ TC + D + + C + GPCR R+FY+ + C F YGGC G+ NNF + A C C
Sbjct: 359 EDESICRFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLSDCQKFCIRGLKSTAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLELDADICKHPPNVGTCT---GRIQRWYFDRPARKCKVLLYSGCGGN---GNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGNGNNFESREVCMNTCPN-----DDTSHLPPNPCFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGNANNFESFASCMVKC 698 HSP 4 Score: 95.1301 bits (235), Expect = 2.732e-16 Identity = 79/284 (27.82%), Postives = 115/284 (40.49%), Query Frame = 0
Query: 358 GRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG-CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA--EVRADGSILTNASI-----------CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
G+ + R CL+ G C + + F+ + +C IY GCGG+ N F S E C+ +C +A + F P ++SV +T C +G + S C + ++ L +F E C +RG C RF+Y+ + C F +SGC GN NNF+S C C D T A C P+ G C+ R++YN E CE F Y GC G+ NNF + C+ TCL + D
Sbjct: 232 GQEVAAPVYRLDEKCLEVPEPGPCDDQLERWHFDAEDRRCKILIYGGCGGNSNNFPSKEICEKKCNAAVA-----ANRF-----CPDWSSVLTDENGVTFECLTKTCP----LGYKCFISGNEGYCCANADTNDLAAPLLFEDESICRFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLSDCQKFCIRGLKSTAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLELDAD 501 HSP 5 Score: 77.7962 bits (190), Expect = 6.092e-11 Identity = 48/112 (42.86%), Postives = 66/112 (58.93%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
C RGPC +L R+++N KCE+F++GGC+GN NNF + +C C K D C P G C G +++FD C +F+Y GCGGNGNNF +L EC++TC
Sbjct: 1049 CDYPKERGPCSGHLRRWYWNKVRSKCESFLYGGCRGNANNFGTEAKCLAEC---KGLPADVCQHPKDVGVCKGYFRRWYFDSSVGWCKQFIYGGCGGNGNNFATLQECRTTC 1157 HSP 6 Score: 72.4034 bits (176), Expect = 3.324e-9 Identity = 63/204 (30.88%), Postives = 89/204 (43.63%), Query Frame = 0
Query: 508 ERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREV---DAFIQF--LAEESETFHEESTSSGVVVAEIFIGIALSGLVILGVYFGFRLILEQVLVKEMQSHGSGLVFSDHFGQKIELTDS 706
ERG C G L R+Y+ + C SF + GC GN NNF + CLA C A +C+ PK G C+ R++++ S+ C++F YGGC G+ NNF T +C TCL+ DA ++ L V+ +G A GL + G I E V Q+ G G S+H + S
Sbjct: 1054 ERGPCSGHLRRWYWNKVRSKCESFLYGGCRGNANNFGTEAKCLAECKGLP----------ADVCQHPKDVGVCKGYFRRWYFDSSVGWCKQFIYGGCGGNGNNFATLQECRTTCLSGRTKVDDATAEYAPLPPAGADLSNYGREHAGTVSTTSVGTA--GLGVYGT-----TISEYV---RTQTGGYGTPESEHVVHTVSTIPS 1237
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|1316134575|ref|XP_023226201.1| (papilin-like [Centruroides sculpturatus]) HSP 1 Score: 224.557 bits (571), Expect = 2.095e-58 Identity = 180/648 (27.78%), Postives = 279/648 (43.06%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRS--LKN-LTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLR-PQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSP-CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLL--KGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQI-APRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVAEIFIGIA 659
C PE G C Y RY++++ G C+ F + GC N+NNF+ + CE C + + QE + L N +L+ FE +I + A L++ S D ++ L F + L HP V + +N + N ++ ++ + STI + + ++ + L + A P C+L G C RF+Y+ + + C+ FV+GGC GN NN+++ EEC C E + D L +G C +++++ ++ C KF+Y GCGGN NNF+S C+ C + P + VP C +P G C + +++TE +C +IY GCGG+DN F + E C+ +C Q C+ + PC Y + Y EE+ C V GC N F T +CE TC + S V C G C + PRFYY C SF + GC GN NNF++ C TC + D C + GPC R++Y+ + +TC+EF YGGC+G+ NN+ T +C TC + E+ A F ++ + + V +I IA
Sbjct: 52 CQQKPETGPCRGYFPRYYYDEEKGECKKFVYGGCKGNDNNFQTVEDCETTCKTQGSQPRRVCFQEKVVASPLGNRCSLNLGAVFEQLTAEQPGMIYF---YAVALDV-SADLKTKIMISNLSIANNFFSNRCFLKHPFHVYL--------------------KNDNRNHIFNKVLCIKAD-----STIHSFTYFSSRSNMKVLLILCTVALAYASKPSCRLEPETGLCRARFPRFYYDPEDDTCKKFVYGGCGGNDNNYETEEECLADCRETEYSVDSVAYCHLKPERGMCLAYMPRYYYNAKTRKCEKFIYGGCGGNANNFESREACEDKC---IQPEV-----EFAEVPV-----CEQPAEPGMCLAYMPRFYYDKTEGKCKKFIYGGCGGNDNNFRTLEECEQQCGAQYYESTCQQKPETGPCR---GYFPRYYYDEEKGECKKFVYGGCKGND---NNFQTVEDCETTCKTQGSQPRRV--------CFQEKVVGPCRAAFPRFYYNQKSGRCESFIYGGCQGNENNFETLDECQDTCEVSSAEVD---------CSSEPVTGPCLAYFRRYYYDQNTQTCKEFVYGGCQGNGNNYKTEKECLQTCASVELCA-KNFYLQDRQLLRLSKKMASKRVKQIRRDIA 633 HSP 2 Score: 195.282 bits (495), Expect = 1.534e-48 Identity = 128/421 (30.40%), Postives = 187/421 (44.42%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLF----------LSHEACKNRCEIQIAPRCRIEHTFE---------------PCLPSPSYTSVWVYSEEQRMCVVTV-GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
S CQ GPC Y R++Y+ + +C+ FV+GGCKGN NNF+++E+CETTC + C + G C + +F+++ +S C F+Y GC GN NNF++L ECQ TC+ + DC +G C Y ++Q C ++Y GC G+ N + S E C +Q R+ P S S+ Y R+ +T+ SN K + L + + +P C L PE G C PRFYY+P D+TC F + GC GN NN+++ + CLA C + E D + + C +G C PR++YN CE+F YGGC G+ NNF +R CE C+ EV+
Sbjct: 424 STCQQKPETGPCRGYFPRYYYDEEKGECKKFVYGGCKGNDNNFQTVEDCETTCKTQGSQPRRVCFQEKVVGPCRAAFPRFYYNQKSGRCESFIYGGCQGNENNFETLDECQDTCE-----------------VSSAEVDCSSEPVTGPCLAYFRRYYYDQNTQTCKEFVYGGCQGNGNNYKTEKECLQTCASVELCAKNFYLQDRQLLRLSKKMASKRVKQIRRDIAKDWPKFVSKKINSIPSYKSGFRIRYLTLLQVSNMKVL------------LILCTVALAYASKP-----SCRLEPETGLCRAYFPRFYYDPEDDTCKKFVYGGCGGNDNNYETEEECLADCRETEYSVD-----SVAYCHLKPERGMCLAYMPRYYYNAKTRKCEKFIYGGCGGNANNFESREACEDKCVQPEVE 805 HSP 3 Score: 175.252 bits (443), Expect = 6.951e-42 Identity = 123/391 (31.46%), Postives = 182/391 (46.55%), Query Frame = 0
Query: 244 CDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQG-VYGFNQTENQCVPYIYS-----GCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALE-GCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
C Y+ RF+Y+ KC+ F++GGC GN NNF++LEECE C E C G C G ++++D + C KFVY GC GN NNF ++ +C++TCK T S R + K S P+ C L G V+ E + Y Y+ + +S+ + N + RC ++H F V++ ++ + V C + F+ S R+ + V +A + C L PE G C PRFYY+P D+TC F + GC GN NN+++ + CLA C + E D + + C +G C PR++YN CE+F YGGC G+ NNF +R CE C+ EV+
Sbjct: 2 CLAYMPRFYYDKTEGKCKKFIYGGCGGNDNNFRTLEECEQQCGAQYYE--STCQQKPETGPCRGYFPRYYYDEEKGECKKFVYGGCKGNDNNFQTVEDCETTCK-----TQGSQPRRVCFQEKVVAS----PL-GNRCSLNLGAVFEQLTAEQPGMIYFYAVALDVSADLKTKIMISNLSIANN---FFSNRCFLKHPFH----------VYLKNDNRNHIFNKVLCIKADSTIHSFTYFS--SRSNMKVLLILCTVALAYASKPSCRLEPETGLCRARFPRFYYDPEDDTCKKFVYGGCGGNDNNYETEEECLADCRETEYSVD-----SVAYCHLKPERGMCLAYMPRYYYNAKTRKCEKFIYGGCGGNANNFESREACEDKCIQPEVE 360 HSP 4 Score: 167.548 bits (423), Expect = 2.267e-39 Identity = 160/627 (25.52%), Postives = 250/627 (39.87%), Query Frame = 0
Query: 14 VEHVIPEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIE-SWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSE-----HKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRV------PKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCE-----IQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
VE C E G C Y R++++++ G C+ F + GCG N+NNFR + CE+ C G E +Q+P F + + D + + + Y N + + +C + QG P +V E + R + R SFI ++ TL +SS+ + + P GPC Y R++Y+ T+ C+ FV+GGC+GNGNN+K+ +EC TC+ D + L K S ++ D + KFV +S+ +S + + + + S C +G C+ + ++ ++ C ++Y GCGG+DN + + E C C + C ++ CL +Y + Y+ + R C + GC N F + CE C+ +V P+ C E G C +PRFYY+ + C F + GC GN NNF F+ C C + + GS +C Q K+ GPCR PRF+YN CE F YGGC+G+ NNF T +C TC
Sbjct: 359 VEFAEVPVCEQPAEPGMCLAYMPRFYYDKTEGKCKKFIYGGCGGNDNNFRTLEECEQQC--GAQYYESTCQQKPETG--PCRGYFPRYYYDEEKGECKKFVYGGCKGNDNNFQTVEDCETTC----KTQG--------SQPRRVCFQEKVVGPCRAAFPRFYYNQKSGRCESFIY--------------GGCQGNENNFETLDECQDTCEVSSAEVDCSSEP-----VTGPCLAYFRRYYYDQNTQTCKEFVYGGCQGNGNNYKTEKECLQTCASVELCAKNFYLQDRQLLRLSKKMASKRVKQIRRDI-AKDWPKFV-------SKKINSIPSYKSGFRIRYLTLLQVSNMKVLLILCTVALAYASKPSCRLEPETGLCRAYFPRFYYDPEDDTCKKFVYGGCGGNDNNYETEEECLADCRETEYSVDSVAYCHLKPERGMCL---AYMPRYYYNAKTRKCEKFIYGGCGGN---ANNFESREACEDKCVQPEVEFADV-PV-----CEQPVEPGKCLAFMPRFYYDKTEGRCRKFIYGGCGGNDNNFLFFEECEKQCGETQ----GS--QPRRVCFQEKVVGPCRAAFPRFYYNQKSGRCESFIYGGCQGNENNFETLDECRDTC 924 HSP 5 Score: 164.851 bits (416), Expect = 1.803e-38 Identity = 151/531 (28.44%), Postives = 236/531 (44.44%), Query Frame = 0
Query: 37 RYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLT----LDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAG-PLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSP-CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLL--KGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIA-PR--CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
R+++NQ SG CE+F + GC NENNF C+ C E+ E S + +T F + + D + + Y G N + ++CA L + F +L+ ++ + A ++ + WP + +N + ++ +R T+ S L L + A P C+L G C Y RF+Y+ + + C+ FV+GGC GN NN+++ EEC C E + D L +G C +++++ ++ C KF+Y GCGGN NNF+S C+ C + P + VP C +P+ G C + +++TE +C +IY GCGG+DN FL E C+ +C E Q + PR C E PC + + Y+++ C + GC + N F T EC TC + S V+ C PE G C R+YY+ ETC F + GC GNGNN+++ + CL TC
Sbjct: 502 RFYYNQKSGRCESFIYGGCQGNENNFETLDECQDTC--------EVSSAEVDCSSEPVTGPCLAYFRRYYYDQNTQTCKEFVYGGCQGNGNNYKTEKECLQTCASVELCAKNFYLQDRQLL---RLSKKMASKRVKQIRRDIAKDWPKFVSKK---INSIPSYKSGFRIRYLTLLQVSNMKVL------LILCTVALAYASKPSCRLEPETGLCRAYFPRFYYDPEDDTCKKFVYGGCGGNDNNYETEEECLADCRETEYSVDSVAYCHLKPERGMCLAYMPRYYYNAKTRKCEKFIYGGCGGNANNFESREACEDKC---VQPEV-----EFADVPV-----CEQPVEPGKCLAFMPRFYYDKTEGRCRKFIYGGCGGNDNNFLFFEECEKQCGETQGSQPRRVCFQEKVVGPCRAA---FPRFYYNQKSGRCESFIYGGCQGNE---NNFETLDECRDTC---QVSSAAVD-------CSSEPETGLCDALFRRYYYDDESETCKEFVYGGCGGNGNNYRTKEECLKTC 983 HSP 6 Score: 127.102 bits (318), Expect = 2.378e-26 Identity = 100/326 (30.67%), Postives = 150/326 (46.01%), Query Frame = 0
Query: 303 GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC---EIQIAPRCRIEHTFEP--CLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
G C +F++D + TC KFVY GCGGN NN+++ EC + C++ + +++S+ + P+R + P Y +N +C +IY GCGG+ N F S EAC+++C E++ A E EP CL ++ + Y + + C + GC N F ECE+ C E + C G C + PRFYY C SF + GC GN NNF++ C TC + D S +C+ R++Y+ ETC+EF YGGC G+ NN+ T+ +C TC
Sbjct: 693 GLCRAYFPRFYYDPEDDTCKKFVYGGCGGNDNNYETEEECLADCRE-TEYSVDSVA-YCHLKPERGMCLAYMP-----------RYYYNAKTRKCEKFIYGGCGGNANNFESREACEDKCVQPEVEFADVPVCEQPVEPGKCL---AFMPRFYYDKTEGRCRKFIYGGCGGND---NNFLFFEECEKQCG-------ETQGSQPRRVCFQEKVVGPCRAAFPRFYYNQKSGRCESFIYGGCQGNENNFETLDECRDTCQVSSAAVDCSSEPETGLCDALFR---------RYYYDDESETCKEFVYGGCGGNGNNYRTKEECLKTC 983
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|669328881|gb|KFD69068.1| (hypothetical protein M514_18645 [Trichuris suis]) HSP 1 Score: 213.001 bits (541), Expect = 7.298e-58 Identity = 131/418 (31.34%), Postives = 200/418 (47.85%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR----CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSD----RSHSLE------VEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC---------HDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
+PC+L GPC R++Y+W ++C F +GGC+GN NN+ S+EECE TC + + ++ +PC P G C K++++ +S C FVY GC GN N FD+L +C++ C + + + C+ P SG C+ + Y ++ +CV + Y GC G+ N + S + C+ C + P+ C + PC+ +Y + + Y++E + C V GC + N F T ECE C R +LE E C L + C GS PR+YY+ + C FT+ GC GN NN+ S Q C ATC + + CE P GPC+ +++YN + CE F YGGC+G+ N F T +CEA C ++
Sbjct: 21 NPCKLPAETGPCRGNFPRYYYDWDLQQCLEFTYGGCEGNANNYDSVEECEATCPDGEHKEQNPCELPADSGPCMAYFTKYYYNKESKKCETFVYGGCAGNENRFDTLEDCEAMCIEEEE------------------NPCILPADSGPCKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPGEEEPKDEDPCELPADSGPCM---AYFTKFYYNKESKKCETFVYGGCQGNE---NRFDTLEECEAKCAEQNAKVRFDTLEECEAKCAEQKKEESPCKLPADLRICRGSFPRYYYDWDLKQCREFTYGGCKGNANNYASLQECEATCPTEEHKEEPKEEPKEEPKEEPKVENPCELPSDSGPCKAFFKKYYYNKESKKCETFVYGGCQGNENRFDTLEECEAKCAEQK 414 HSP 2 Score: 171.785 bits (434), Expect = 5.194e-43 Identity = 115/388 (29.64%), Postives = 174/388 (44.85%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIA-----------PRCRIEHTFE-PC-LPS---------PSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG--------CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYN 590
+PC+L GPC Y +++YN +++KCE FV+GGC GN N F +LE+CE C E E+++PCI P G C G ++++D+ C +F Y GC GN NN+ SL EC++TC +P + C P SG C + +N+ +C ++Y GC G++N F + E C+ +C Q A +C + E PC LP+ P Y W + + GC K N +++ ECE TC ++ E C L + G C ++YY + C +F + GC GN N F + + C A C + + R CE P GPC +++YN
Sbjct: 82 NPCELPADSGPCMAYFTKYYYNKESKKCETFVYGGCAGNENRFDTLEDCEAMCIE---EEENPCILPADSGPCKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPGEEEP--------------KDEDPCELPADSGPCMAYFTKFYYNKESKKCETFVYGGCQGNENRFDTLEECEAKCAEQNAKVRFDTLEECEAKCAEQKKEESPCKLPADLRICRGSFPRYYYDWDLKQCREF--TYGGC---KGNANNYASLQECEATCPTEEHKEEPKEEPKEEPKEEPKVENPCELPSDSGPCKAFFKKYYYNKESKKCETFVYGGCQGNENRFDTLEECEAKCAEQKNR--------RRPCELPADSGPCMAYFTKYYYN 439 HSP 3 Score: 166.007 bits (419), Expect = 5.734e-41 Identity = 108/345 (31.30%), Postives = 161/345 (46.67%), Query Frame = 0
Query: 291 EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPS-PSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC--HDAEVRADGSILTNASICEQPKLKGP---------CRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
++++PC P G C G+ ++++D+ C +F Y GC GN NN+DS+ EC++TC D G H + P C P SG C Y +N+ +C ++Y GC G++N F + E C+ C + C + PC P Y W + + CV GC N +++ ECE TC + E + C L + G C +FYY + C +F + GC GN N F + + C A C +A+VR D A EQ K + P CR PR++Y+ ++ C EF YGGCKG+ NN+ + +CEATC
Sbjct: 18 QEENPCKLPAETGPCRGNFPRYYYDWDLQQCLEFTYGGCEGNANNYDSVEECEATCPD---------GEHKEQNP------CELPADSGPCMAYFTKYYYNKESKKCETFVYGGCAGNENRFDTLEDCEAMCIEEEENPCILPADSGPCKGKFPRYYYDW----DLKKCVEFTYGGCEGN---ANNYASLQECEATCPGEEEPKDE-------DPCELPADSGPCMAYFTKFYYNKESKKCETFVYGGCQGNENRFDTLEECEAKCAEQNAKVRFDTLEECEAKCAEQKKEESPCKLPADLRICRGSFPRYYYDWDLKQCREFTYGGCKGNANNYASLQECEATC 333 HSP 4 Score: 129.028 bits (323), Expect = 4.083e-28 Identity = 85/269 (31.60%), Postives = 123/269 (45.72%), Query Frame = 0
Query: 372 CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC---EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAE 635
C P +G C+ Y ++ QC+ + Y GC G+ N + S E C+ C E + C + PC+ +Y + + Y++E + C V GC+ + N F T +CE C+ + + C+L + G C G PR+YY+ + C FT+ GC GN NN+ S Q C ATC E D CE P GPC +F+YN + CE F YGGC+G+ N F T +CEA C + L E
Sbjct: 23 CKLPAETGPCRGNFPRYYYDWDLQQCLEFTYGGCEGNANNYDSVEECEATCPDGEHKEQNPCELPADSGPCM---AYFTKYYYNKESKKCETFVYGGCAGNE---NRFDTLEDCEAMCIEEEENP-----------CILPADSGPCKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPGEEEPKD------EDPCELPADSGPCMAYFTKFYYNKESKKCETFVYGGCQGNENRFDTLEECEAKCAEQNAKVRFDTLEE 268
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|669225304|emb|CDW53418.1| (papilin [Trichuris trichiura]) HSP 1 Score: 222.246 bits (565), Expect = 4.427e-57 Identity = 149/470 (31.70%), Postives = 210/470 (44.68%), Query Frame = 0
Query: 216 STTPASTLSSSTSELAGSP------CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHM--DPTMNSLGRHIFRVPKR------SLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTS--VWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGS----------------ILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVV 651
S P L+++ ++ P C GPC R++YN EKCE F++ GC GN NNF S+ C+ TC E D C P G+C+G ++++FD C +YSGCGGNGNNFDS A C+ C + D + +S + VP + L+ C P +G C + + +++ E C + YSGCGG+ N F S E C N C + + F P P S W Y MC GC N F + S C C P CL +RG C G RF+Y+ +TC F + GC G GNNF + Q CL C AE + IL C+QPK++G C + + R+F+N + CE F Y GC G+RNNF T +QCE C + + + ++++ F E+ S V
Sbjct: 414 SPAPEDALATTPHFVSDPPPKIENKCNFPKDAGPCKENHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLELD---ADICKHPPNVGTCTGRIQRWYFDRWVRKCKPLLYSGCGGNGNNFDSQAICERYCLPELSSDVSASSQPVRVTMVPHKVLPTVLPLNRCFHPKNAGTCTGQFRRWYYDELEEMCKAFTYSGCGGNGNNFESREVCMNTCPNDDSSHLPLNPCFHRRDPGPCRASHRRWFYDSRVNMCHPFTYGGCGGN---ANNFESFSSCMVKC----------GPEILSSRCLHPTDRGDCSGHFVRFHYDSDSKTCKEFVYGGCGGTGNNFATKQECLKACAKAEPEDESKKFLKAVHTPVSIQPTLILPTEDSCKQPKMRGNCNKYELRYFFNAHVNRCEFFFYSGCGGNRNNFKTLSQCELFCSKKRPNPLLFGSSDDATPFSSETYGSTETV 867 HSP 2 Score: 193.741 bits (491), Expect = 1.124e-47 Identity = 135/436 (30.96%), Postives = 191/436 (43.81%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC--------------------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-------CRIEHTFEPCLPS---------PSYTSV-------WVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C+ RGPC + +RFFY+ + +C F + GC GN NNFKSL +C+ C S+ P+ ++ C P G C + ++++++ C F+YSGCGGN NNFDS+ CQ TC + +D + C P G C + F++ +C P +YSGCGG+ N F S C+ C +++ R+ LP+ P W Y E + MC GC GN F + C TC +D S L + P C R + G C S R++Y+ CH FT+ GC GN NNF+SF C+ C IL+ S C P +G C RF Y+ +TC+EF YGGC G+ NNF T+ +C C E
Sbjct: 358 CKFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLLDCQKFCMRGVKSPAPEDALATTPHFVSDPPPKIENKCNFPKDAGPCKENHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLE-LDADI-----------------CKHPPNVGTCTGRIQRWYFDRWVRKCKPLLYSGCGGNGNNFDSQAICERYCLPELSSDVSASSQPVRVTMVPHKVLPTVLPLNRCFHPKNAGTCTGQFRRWYYDELEEMCKAFTYSGCGGN---GNNFESREVCMNTCPNDDSSHLPLNP------CFHRRDPGPCRASHRRWFYDSRVNMCHPFTYGGCGGNANNFESFSSCMVKCGP-------EILS--SRCLHPTDRGDCSGHFVRFHYDSDSKTCKEFVYGGCGGTGNNFATKQECLKACAKAE 757 HSP 3 Score: 99.3673 bits (246), Expect = 1.421e-17 Identity = 78/270 (28.89%), Postives = 114/270 (42.22%), Query Frame = 0
Query: 372 CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG-CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCH---DAEVRADGSILTNASI----------CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
CL+ G C + + F+ + +C IY GCGG+ N F S E C+ +C +A + F P ++SV +T C +G + S C + ++ L +F E C +RG C RF+Y+ + C F +SGC GN NNF+S C C + D T + C PK GPC+ + R++YN E CE F Y GC G+ NNF + C+ TCL + D
Sbjct: 240 CLETPEPGPCDDQLERWHFDAEDRRCKILIYGGCGGNSNNFPSKEICEKKCNAAVA-----ANRF-----CPDWSSVLTDETGVTFECLTKTCP----LGYKCFISGNEGYCCANADTNDLAAPLLFEDESICKFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLLDCQKFCMRGVKSPAPEDALATTPHFVSDPPPKIENKCNFPKDAGPCKENHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLELDAD 495 HSP 4 Score: 79.337 bits (194), Expect = 2.162e-11 Identity = 49/112 (43.75%), Postives = 67/112 (59.82%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
C RGPC +L R+++N KCE+F++GGC+GN NNF + +C C K D C P G+C G +++FD C +FVY GCGGNGNNF +L EC++TC
Sbjct: 1036 CDYPKERGPCSGHLRRWYWNKLRSKCESFLYGGCRGNANNFDTEAKCLAEC---KGLPADVCQHPKDVGTCKGYFHRWYFDSNIGWCKRFVYGGCGGNGNNFATLQECRTTC 1144 HSP 5 Score: 72.7886 bits (177), Expect = 2.357e-9 Identity = 46/125 (36.80%), Postives = 65/125 (52.00%), Query Frame = 0
Query: 499 ALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
++ C ERG C G L R+Y+ + C SF + GC GN NNF + CLA C A +C+ PK G C+ R++++ +I C+ F YGGC G+ NNF T +C TCL+
Sbjct: 1032 SMHKCDYPKERGPCSGHLRRWYWNKLRSKCESFLYGGCRGNANNFDTEAKCLAECKGLP----------ADVCQHPKDVGTCKGYFHRWYFDSNIGWCKRFVYGGCGGNGNNFATLQECRTTCLS 1146 HSP 6 Score: 70.8626 bits (172), Expect = 9.453e-9 Identity = 54/181 (29.83%), Postives = 73/181 (40.33%), Query Frame = 0
Query: 494 VEPMFAL-EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRAD------GSILTNA---------------------------------------------SICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
V P++ L E CL PE G C L R++++ D C + GC GN NNF S + C C +A V A+ S+LT+ SIC+ PK +GPC RFFY+ C F Y GC G+RNNF + C+ C+
Sbjct: 230 VAPVYRLDEKCLETPEPGPCDDQLERWHFDAEDRRCKILIYGGCGGNSNNFPSKEICEKKC-NAAVAANRFCPDWSSVLTDETGVTFECLTKTCPLGYKCFISGNEGYCCANADTNDLAAPLLFEDESICKFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLLDCQKFCM 409
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|669309056|gb|KFD52549.1| (hypothetical protein M513_06583 [Trichuris suis]) HSP 1 Score: 220.705 bits (561), Expect = 7.829e-57 Identity = 154/490 (31.43%), Postives = 219/490 (44.69%), Query Frame = 0
Query: 212 TTLPSTTPAST--LSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKR------SLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC---EIQIAP--------------RCRIEH-TFEPCL----PSPSYTS--VWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAE--------VRADGS--------ILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVV 651
+T P PA+T + C G C R++YN EKCE F++ GC GN NNF S+ C+ TC E D C P G+C+G ++++FD + C +YSGCGGNGNNFDS A C+ C + +NS + VP + L+ C P G C + + +++ E C P+ Y+GCGG+ N F S E C N C + AP + H PC P P S W Y + MC GC+ N F + + C C P CL +RG C G R++Y+ +TC F + GC G GNNF + Q CL C +AE ++A+ + IL C+QPK++G C + + R+F+N + CE F Y GC G+RNNF T +QCE C R + + ++++ F ES S V
Sbjct: 420 STAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLELD---ADICKHPPNVGTCTGRIQRWYFDRPARKCKVLLYSGCGGNGNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGNGNNFESREVCMNTCPNAKKTTAPTTVATLPAMTTLPFKDDTSHLPPNPCFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGN---ANNFESFASCMVKC----------GPEILSSRCLHPTDRGDCSGHFVRYHYDSDSKTCKEFVYGGCGGTGNNFATKQECLRACANAEADDEVKKLLKAERTPVSIQPTLILPTEDSCKQPKMRGNCNKYELRYFFNAQVGRCEFFFYSGCGGNRNNFKTLSQCELFCSKRRPNPLLFGSSDDATAFSSESYGSTEAV 893 HSP 2 Score: 188.734 bits (478), Expect = 3.580e-46 Identity = 134/464 (28.88%), Postives = 198/464 (42.67%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC--------------------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSV---------------------WVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLE--VEPMFAL--------------EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESET 639
C+ RGPC + +RFFY+ + +C F + GC GN NNFKSL +C+ C + P+ ++ C P GSC S ++++++ C F+YSGCGGN NNFDS+ CQ TC + +D + C P G C + F++ +C +YSGCGG+ N F S +C+ C +++ + +P +V W Y E + MC GC GN F + C TC + + + V + A+ C R + G C S R++Y+ CH FT+ GC GN NNF+SF C+ C IL+ S C P +G C R+ Y+ +TC+EF YGGC G+ NNF T+ +C C N E D ++ L + T
Sbjct: 364 CRFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLSDCQKFCIRGLKSTAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLE-LDADI-----------------CKHPPNVGTCTGRIQRWYFDRPARKCKVLLYSGCGGNGNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGN---GNNFESREVCMNTCPNAKKTTAPTTVATLPAMTTLPFKDDTSHLPPNPCFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGNANNFESFASCMVKCGP-------EILS--SRCLHPTDRGDCSGHFVRYHYDSDSKTCKEFVYGGCGGTGNNFATKQECLRACANAEADDEVKKLLKAERT 797 HSP 3 Score: 176.792 bits (447), Expect = 3.073e-42 Identity = 115/368 (31.25%), Postives = 165/368 (44.84%), Query Frame = 0
Query: 291 EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHM-----DPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPM-------------FALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA-EVRADGSILTNASICEQP----------------KLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
E + C P +G C +F +D + C F YSGCGGN NNF SL++CQ C + D + R + P + + C P +G C+ + +N E +C P+IYSGCGG+ N F S C+ C A C+ C W + R C V + GC GN F + + CER CL + S + +P+ L C GSC G R+YY+ ++E C FT++GC GNGNNF+S + C+ TC +A + A ++ T ++ P + GPCR R+FY+ + C F YGGC G+ NNF + A C C
Sbjct: 359 EDESICRFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLSDCQKFCIRGLKSTAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLELDADICKHPPNVGTCT---GRIQRWYFDRPARKCKVLLYSGCGGN---GNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGNGNNFESREVCMNTCPNAKKTTAPTTVATLPAMTTLPFKDDTSHLPPNPCFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGNANNFESFASCMVKC 720 HSP 4 Score: 142.51 bits (358), Expect = 3.899e-31 Identity = 123/436 (28.21%), Postives = 175/436 (40.14%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPL------RPQGFSKSHPKLM----------HPIQVDRNEAITITLSLKLLRIESWP------GRRNNSF----IVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPE-KDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCK-----DHMDPTMNSLGRHIFRVPKRSL---SDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
C + G C +R+++N CE F +SGCG N NNF C+ C+ + +I + P ++ T + + D +++ Y+ G N S R C L +P S K++ HP V L P G N+F + MN ++ P+T+AT TTLP TS L +PC GPC R+FY+ K C F +GGC GN NNF+S C C PE C+ P +G CSG ++H+D S TC +FVY GCGG GNNF + EC C D + + + + P L C +P G+C + Y FN +C + YSGCGG+ N F + C+ C
Sbjct: 445 CNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLE---LDADICKHPP--NVGTCTGRIQRWYFDRPARKCKVLLYSGCGGNGNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGNGNNFESREVCMN--TCPNAKKTTAPTTVATLPAMTTLP-------FKDDTSHLPPNPCFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGNANNFESFASCMVKCG---PEILSSRCLHPTDRGDCSGHFVRYHYDSDSKTCKEFVYGGCGGTGNNFATKQECLRACANAEADDEVKKLLKAERTPVSIQPTLILPTEDSCKQPKMRGNCNKYELRYFFNAQVGRCEFFFYSGCGGNRNNFKTLSQCELFC 863 HSP 5 Score: 96.2857 bits (238), Expect = 1.133e-16 Identity = 82/287 (28.57%), Postives = 118/287 (41.11%), Query Frame = 0
Query: 358 GRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG-CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA--EVRADGSILTNASI-----------CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFI 630
G+ + R CL+ G C + + F+ + +C IY GCGG+ N F S E C+ +C +A + F P ++SV +T C +G + S C + ++ L +F E C +RG C RF+Y+ + C F +SGC GN NNF+S C C D T A C P+ G C+ R++YN E CE F Y GC G+ NNF + C+ TCL E+DA I
Sbjct: 232 GQEVAAPVYRLDEKCLEVPEPGPCDDQLERWHFDAEDRRCKILIYGGCGGNSNNFPSKEICEKKCNAAVA-----ANRF-----CPDWSSVLTDENGVTFECLTKTCP----LGYKCFISGNEGYCCANADTNDLAAPLLFEDESICRFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLSDCQKFCIRGLKSTAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCL--ELDADI 502 HSP 6 Score: 77.411 bits (189), Expect = 7.295e-11 Identity = 48/112 (42.86%), Postives = 66/112 (58.93%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
C RGPC +L R+++N KCE+F++GGC+GN NNF + +C C K D C P G C G +++FD C +F+Y GCGGNGNNF +L EC++TC
Sbjct: 1053 CDYPKERGPCSGHLRRWYWNKVRSKCESFLYGGCRGNANNFGTEAKCLAEC---KGLPADVCQHPKDVGVCKGYFRRWYFDSSVGWCKQFIYGGCGGNGNNFATLQECRTTC 1161 HSP 7 Score: 73.1738 bits (178), Expect = 1.431e-9 Identity = 101/405 (24.94%), Postives = 149/405 (36.79%), Query Frame = 0
Query: 28 RGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESW-----PGRRNNSFIVM-----NDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
RG+C YE RYFFN G CE F +SGCG N NNF+ CE C RR L D D + SY S A ++SP + L + P P+ + + +L E+ PG S + L LQT+ +P++IAT+ T+T P ++ + A P +L + G + G G +F+ HK C P +G CSG +++++ C F+Y GC GN NNF + A+C + CK L + C P G C+ + F+ + C +IY GCGG+ N F + + C+ C
Sbjct: 819 RGNCNKYELRYFFNAQVGRCEFFFYSGCGGNRNNFKTLSQCELFCSK-------------RRPNPLLFGSSD------DATAFSSESYGSTEA----VHSPSANAYPPGSSLPSD--HREEPSTSSPVTQYSGRLQSSSTTLSGTEKETSYTFLPPGYTEGSLLSQYPGNRPSLPGLQTHHVKKPASIATNRPTSTY--VGPDASQQAKEDVSAYEPGELPLQPGVSETTAKAKPPVSPAPPPMPPGSAGVAEAGTSFR-----------HK------CDYPKERGPCSGHLRRWYWNKVRSKCESFLYGGCRGNANNFGTEAKCLAECK--------GLPADV----------CQHPKDVGVCKGYFRRWYFDSSVGWCKQFIYGGCGGNGNNFATLQECRTTC 1161 HSP 8 Score: 72.0182 bits (175), Expect = 3.196e-9 Identity = 46/121 (38.02%), Postives = 64/121 (52.89%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
C ERG C G L R+Y+ + C SF + GC GN NNF + CLA C A +C+ PK G C+ R++++ S+ C++F YGGC G+ NNF T +C TCL+
Sbjct: 1053 CDYPKERGPCSGHLRRWYWNKVRSKCESFLYGGCRGNANNFGTEAKCLAECKGLP----------ADVCQHPKDVGVCKGYFRRWYFDSSVGWCKQFIYGGCGGNGNNFATLQECRTTCLS 1163 The following BLAST results are available for this feature:
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 14
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BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of kunitz-like protease inhibitor precursor vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold580_size130538:108918..111884+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold580_size130538-snap-gene-0.29 ID=maker-scaffold580_size130538-snap-gene-0.29|Name=kunitz-like protease inhibitor precursor|organism=Tigriopus kingsejongensis|type=gene|length=2967bp|location=Sequence derived from alignment at scaffold580_size130538:108918..111884+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'kunitz-like protease inhibitor precursor' has the following synonyms
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