BLAST of hypothetical protein HMPREF1544_09400 vs. L. salmonis genes
Match: EMLSAG00000000679 (supercontig:LSalAtl2s:LSalAtl2s1107:264170:270518:1 gene:EMLSAG00000000679 transcript:EMLSAT00000000679 description:"maker-LSalAtl2s1107-augustus-gene-1.38")

HSP 1 Score: 776.933 bits (2005), Expect = 0.000e+0
Identity = 518/1400 (37.00%), Postives = 728/1400 (52.00%), Query Frame = 0

Query:  254 LNCWQCPSCPALYRQQSSFKQHASADHALSLVEVLAVEPIQMTLAEFTRRKVSRSQRPVGNVVATTSKTFPLDKSKRKPIGKEWAPNSFSSTFTCFFCNEQFRKDYKLKLHLMLNHKSENAEDMIKAKEELTKSK-LDGCVHKCALCGSKYNSVANFTRHIKDVHNMSRAQYREDYG--SSEVVSRMFKCELCEKEVKHTRNIIGAHMKMVHLISWREYQEILVKIRQGESVGDLPAPELFDCVICG-VSVKYKREHLNKKHQITEDVYDELILKKNRGEDISDSLPARELVRCLICDRECLDFKKHIERSHQITEEMYEEIITSRGLEDQRRSISQFEESLVTNGKAKFSSKSSPGKSNKRSCSDSFGTSSVRSESPGTLQVAGANPLSTDLQCYFGCDEVFKKDYQLYLHLKLKHRSETTDEIARAYEAADEEIALTKRSGSVFQCSLCPKQFNDNGAFYSHIQSKHDMQWRDYKEQY---GRCEVESSP--------------FECKICSRVIKYDRNTIHTHLKNVHGINWTLYLDRLRKMRRGEQPDALPQIEMIECRVCNVQVKYLKDHLRNAHKITEQEYESLFEGEHDLFGSTTQAMELGPSHVESGTSKVKDPITPQMVITDGVNLLSSQPVAIPKPPKSDIQNKTNKACSSCEITFESRRGFIEHCTTVHSMKFKTKSGITISAP----------------TLQHPM------KRKLN-------------SEPIVDLPKRIRRGTDTSGCGQSMGESNISSGST-DRHGGSSATQYTPNGVSKWNQCVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPT----TSGTLVSKSEPKLVASPPAQSVAKPSC-STPLLVGKLSNSSVNLSKEDKWNRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDRVG---GYDSSTAQEVP--FMTHGDDITSEP---SNEQPIIVHSEALVDNGNQP------SRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKYGRVPDEPAIPLQPAANSDEQLSFGSHFLLEENGDDSNDLSNGAA--GPPKAKRVRPSAKIPGGLPCDYCGKIFSNRS-------NCNRHMILSCESRKGGSNEDSNSDVKPSH------------DDTDESQEAESNEAEQAPLE--------KPRQP-----------LKCPYPDCEVTHVRSALLKRHLFDAHNIQNVSVQLPELENDQISAEALSFIQVKSEPSDN 1537
            ++CW C +CP ++R+ +  K+H+   H     + L   P +MT  E+ +    R   PV                                 ++C+FC E FR D++LK HL   HK+E+  +M+KAKEEL K +       KC+LCGS   +     +H+  VHN+++ QY+E++G  S+E   + FKCELC++E++H R  I  HMK  HLISW EYQEIL+KIR+ +  G   + E FDC +CG   +K K+ HL KKHQ+++++YD+L+     G   S S   +    C++C+ E  D +KH+ER H+++ + Y E+ TS   ED        + S++TNG                                      G+N  S  L+C +G ++ F K+YQLYLH +LKH S         Y+  DEE + + + G   +C LC  +F D  +  +HI S HD   +D           +  S+P              FECK+C+R  KYDR  I+ HL+  HGINW  YLDR+R                 +C++C+  VK LK+HL   HK+TE+EYE+                       ES  S  +  ++    ++   +   +    IPKPPKSDIQNKTNK CSSC ITF SRR FIEHCTTVH+MKFKTKSG+TI AP                ++ H +      KRK +             S P +    R+  G D S     + +  +   +  + +  +  + Y   GVS+WNQC YQCCFC K+TQSRSSMTSHI N+HGIPIKEYKE+NYPDIEV T WFQCRLCS +TKFVKDCI+PHLKMSH+++IE YE+  M+PEDWP     + GT   KSE +   +   +S+ KP+  S+P      S+ S   +++ KWN+CRF+C +C+   +D+RQMR HI+  H ++Y+ YV   G++E+VT KF+CELC+SEMK CRQNIYAHMKDVHKITL EYE +VG     D     E P   + + D  T++P    +     +HS+     G QP      S+WNKCR++C ICQ+++SEK  IRDHI+K HG  + DYEA YGD EV TEYF C++C AEVKH  +NISLHLQ VH ++  +Y++   +  + P++    + ++ E   FG+HFLL+ N  DS+  + G++  GPP     R     P    C  C + F+ R        N +R  I      +  S E     V PS             D   +     SN      L          PR P           +KCP  DC+      + L  HL DAH I      L + E+D+   E L+   +K++  DN
Sbjct:  128 ISCWFCSNCPKVFRKSAHLKEHSIEIHKSD--KELDTLPKRMTEEEYYQH---RHGIPV-------------------------------VYYSCYFCKELFRGDHRLKKHLHTEHKNESFAEMVKAKEELRKVRDARKKSVKCSLCGSGXITEPEXEKHLLSVHNLTKTQYQEEFGKISTEATLKRFKCELCDEEIEHVRETIALHMKNSHLISWTEYQEILLKIRRED--GTESSMEPFDCQVCGERRIKNKKYHLRKKHQMSDEIYDKLL-----GHRQSSSSTIKT---CMLCEHETQDLRKHLERIHRVSYDSYMEL-TSESKED--------DSSVMTNG--------------------------------------GSNNHS--LRCRYGXEDXFXKEYQLYLHXRLKHDS---------YDG-DEEGSESNKRGLEEKCYLCRMKFFDVASLMAHISSVHDGPSKDNMSNLSGPNSNDSLSTPKNISPSNNNSIGGSFECKLCNRNFKYDRTAINEHLQAAHGINWLGYLDRIRVK--------------ADCKICDTSVKNLKEHLMTVHKLTEKEYEAR----------------------ESDKSMNQSALSIDQSVSQNADDFLAVETNIPKPPKSDIQNKTNKTCSSCTITFASRRNFIEHCTTVHNMKFKTKSGLTIQAPKSSSSNAPVVNRKAVTSVAHQLTPSVTIKRKFDTNSNTNFSPPNFASSPSLLKRPRVEGGNDLSSILPQLPQHPLPPAAVIEANEKTLGSHYQTTGVSRWNQCKYQCCFCKKTTQSRSSMTSHIANTHGIPIKEYKERNYPDIEVVTNWFQCRLCSTKTKFVKDCISPHLKMSHSIDIEDYERQYMRPEDWPNCQRPSGGT---KSEMRNSETTTTRSLLKPAVPSSP------SHPSDEENEKKKWNKCRFQCKICQLITIDARQMRVHISNAHKLSYDNYVAQYGSSEIVTKKFRCELCSSEMKFCRQNIYAHMKDVHKITLSEYEGKVGYVPPPDDDDIDESPEHIVINSDVGTTKPYDCDDNDGSSMHSQ-----GTQPQQDHFTSKWNKCRYKCQICQRITSEKPVIRDHIMKQHGFDLEDYEAQYGDVEVVTEYFTCSICSAEVKHCHRNISLHLQKVHNMTTSEYDEGMSQ-NESPSV----SKDNGEIGGFGAHFLLDSNSSDSSSPTRGSSSNGPPIRPPPREDIINPKNKYCMPCNRDFNRRQAFVEHCRNVHRMTINFTRDTRADSEEGQVQTVHPSVVTVTTTPGSLNCDYCGKQFSNRSNRNRHMLLSCEVVKAGGVPRTPHQQMIAEDGGSIKCPMSDCDSYFNVGSSLSGHLADAHGI------LDKSEDDE---ERLTASPIKTKDHDN 1358          

HSP 2 Score: 106.686 bits (265), Expect = 3.726e-23
Identity = 109/436 (25.00%), Postives = 191/436 (43.81%), Query Frame = 0

Query:   62 RKTLSCFLCGGTHIYPGPRFENHLMNEHGAVFDLEFIIQLSLFKQEHGRL----PHVEGEPAASNGHWENGNAPTPKSADTQDGFAQTETKPLCSDCQGPVDDPAKSNGSSLRRSKRAPQSKSRSRSRTVPFGFGDEATLDELASLGLDETEEEEEADDEDLSRDEYIGLDPHHYLASQGSIEENGASHLDRAEPALNCWQCPSCPALYRQQSSFKQHASADHA-LSLVEVLAVEPIQMTLAEFTRRKVSRSQRPVGNVVATTSKTFPLD--KSKRKPIGKEWAPNSFSST---FTCFFCNEQFRKDYK-LKLHLMLNHK---SENAEDMIKA-KEELTKSKLDGCVHKCALCGSKYNSVANFTRHIKDVHNMSRAQYREDYGSSEVVSRMFK-CELCEKEVKHTRNIIGAHMKMVHLISWREYQEILVKIRQGES 481
            R TL+C LCGG  IYPGPR++NHLMNEHG + DL F++Q+S+FK++ G L    PH++  P       E+      K   TQ    ++  K  C  C  PV + ++    +    ++     S       P  F   A L E  S+ + ++++E +   + ++ +EY       Y    G              P +  + C  C  L+R     K+H   +H   S  E++  +     + +  ++ V  S    G +     +   L      +    +E+   S  +T   F C  C+E+     + + LH+  +H    +E  E ++K  +E+ T+S ++     C +CG +   + N   H++  H MS   Y +  G  +  S   K C LCE E +  R     H++ +H +S+  Y E+  + ++ +S
Sbjct:   16 RSTLNCILCGGKQIYPGPRYKNHLMNEHGVLLDLTFLLQISIFKRDKGALPDLSPHLDPLPR------EDDEKKASKVISTQ---TESPIKLSCVKCSDPVQESSEEEEENEEDVRKDDGLISCWFCSNCPKVFRKSAHLKE-HSIEIHKSDKELDTLPKRMTEEEY-------YQHRHGI-------------PVVY-YSCYFCKELFRGDHRLKKHLHTEHKNESFAEMVKAKEELRKVRDARKKSVKCSLCGSGXITEPEXEKHLLSVHNLTKTQYQEEFGKISTEATLKRFKCELCDEEIEHVRETIALHMKNSHLISWTEYQEILLKIRREDGTESSMEP--FDCQVCGER--RIKNKKYHLRKKHQMSDEIYDKLLGHRQSSSSTIKTCMLCEHETQDLR----KHLERIHRVSYDSYMELTSESKEDDS 412          

BLAST of hypothetical protein HMPREF1544_09400 vs. nr
Match: gi|1325328627|ref|XP_023346167.1| (uncharacterized protein LOC111715132 [Eurytemora affinis])

HSP 1 Score: 951.429 bits (2458), Expect = 0.000e+0
Identity = 535/1227 (43.60%), Postives = 704/1227 (57.38%), Query Frame = 0

Query:  366 MLNHKSENAEDMIKAKEELTKSKLDGCVHKCALCGSKYNSVANFTRHIKDVHNMSRAQYREDYGSSEVVSRMFKCELCEKEVKHTRNIIGAHMKMVHLISWREYQEILVKIRQGESVGDLPAPELFDCVICGVSVKYKREHLNKKHQITEDVYDELILKKNRGEDISDSLPARELVRCLICDRECLDFKKHIERSHQITEEMYE-----------EIITSRGLE----DQRRSISQFEESLVTNGKAKFSSKSSPGKSNKRSCSDSFGTSSVRSESPGTLQVAGANPLSTDLQCYFGCDEVFKKDYQLYLHLKLKHRSETTDEIARAYEAADEEIALTKRSGSVFQCSLCPKQFNDNGAFYSHIQSKHDMQWRDYKEQYGRCEVESSPFECKICSRVIKYDRNTIHTHLKNVHGINWTLYLDRLRKMRRGEQPDALPQIEMIECRVCNVQVKYLKDHLRNAHKITEQEYESLFEGEHDLFGSTTQAMELGPSHVESGTSKVKDPITPQMVITDGVNLLSSQPVAIPKPPKSDIQNKTNKACSSCEITFESRRGFIEHCTTVHSMKFKTKSGITISAPTLQHPMKRKLNSEPIVDLPKRIRRGTDTSG----------CGQSMGESNISSGSTDRHGGSSATQYTPNGVSKWNQCVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWP------TTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNSSVNLSKE------DKWNRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGYDSSTAQEVPFMTHGDDITSEPSNEQPIIVHSEALVDNGNQPSRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKYGRVPDEPAIPLQPAANSDEQLSFGSHFLLEENGDDSNDLSNGAAGPPKAKRVRPSAKIPGGLPCDYCGKIFSNRSNCNRHMILSCESRKGGSNEDSNSDVKPSHDDTDESQEAESNEAEQAPLEKPRQPLKCPYPDCEVTHVRSALLKRHLFDAHNI-QNVSVQLPELENDQISAEALSFIQVKSEP---------SDNEEDRKVPP 1545
            M+ HK + A  M +AKEEL K+KLDGCVHKC LC +KYNS+ANFTRHIKDVHN+SR QY+ +YGSSE+VSR F CELC+KEVKHTRNIIGAHMKMVHLISW+EYQ+I+ K+++G  + DLP PELFDC ICGVSVKYK+EHLNKKHQI E+VY+ELI KKNRGEDIS+ LP RE+  CLIC+REC+D K+H++  H+++EE Y            ++I    ++    D  R +S      +   + K   KSSP    KR        S V+ E         A PLSTDLQCYF C EVFKKDYQL+LHLKLKHR E  DE+ +AY AA+EEIALT+RS S+F C+LCP++FNDNGAFY HIQ+KHD+ ++DYKE+YGRCE ES PFEC ICS+VIKYDRNTIHTHLKNVHGINWT YL+R+RK RRG  PD LP ++ +ECRVCN+ VKYL+DHLRNAHKITEQEYE LF    D      Q  E      E+G              +  + L     + + +P K +IQNK N+ CS CE  F+SR+ FIEHC  +H MKFKTK+G++I  PT Q  +KR+++       P++  R  +TS           C Q + +  +   S+     +S+       +SKWN C +QC  C K++ S+  +  HI   HG+  + YKE +YPDIE+ + WF CR+C    KF+KD I  H+K +H + +E YE+  M+ EDWP      +  GT + +     V +   QS  K   S         NS  N+S +      D WNRC F+C LC    +D R +RAHI  +H ++Y+ Y+   G  E+ T K+ C++C +E +H R NIY H+++ H +T+D+Y     G   +  +       G   T+                    +  RWNKC F+C +C+K S+EK++IR H+   H   + D E  +GDCE+HTEYFFC +C AEVKH  KNI +HLQ  H L+   YE K                           FL             G     K  R R +      +PC+ CGK FS+ SN  RH    C    G                      AE +  E+  L       +CP   C +   RS  +K+HL + H I QNVSV+L E   D+ +AE      +K EP          D+EE++ + P
Sbjct:    1 MMKHKDQPAHLMEQAKEELIKAKLDGCVHKCGLCNNKYNSIANFTRHIKDVHNISRIQYKSEYGSSEIVSRTFTCELCQKEVKHTRNIIGAHMKMVHLISWKEYQDIIFKLKKGGEMMDLPNPELFDCSICGVSVKYKKEHLNKKHQIDEEVYEELIEKKNRGEDISEDLPDREVFTCLICERECMDLKRHLQVCHKLSEEQYRSEFLNGSSPVAKVIRQESIKPMSTDLLRLVSSELNMNLNQAEKKPEFKSSPPPPLKR-------LSRVKHE---------AEPLSTDLQCYFNCQEVFKKDYQLFLHLKLKHRLEDPDELKKAYAAANEEIALTRRSASLFNCALCPREFNDNGAFYGHIQNKHDISYKDYKEKYGRCETESKPFECNICSKVIKYDRNTIHTHLKNVHGINWTKYLERIRKTRRGVSPDPLPTLKTVECRVCNINVKYLRDHLRNAHKITEQEYEELFTDGSDYKTGKVQIKEEIEESFENGQE------------SGSLEL----DLKVLRPSKLEIQNKGNRNCSVCEFEFDSRKEFIEHCQVIHGMKFKTKTGVSIPPPT-QATIKRRVDEIEQDFQPEKKFRTFETSDGYEIEIEGVPCHQEVSQDFMGETSSSTSQFTSSL------LSKWNLCQFQCAICSKTSNSKKGLMGHIMTHHGLGSRAYKE-SYPDIEINSEWFNCRVCGNSVKFMKDTIVSHIK-THGLNLEKYEREYMEEEDWPYHPNHQSRPGTTLRRESLSSVENISEQSGRKSENS--------ENSDENMSDQPNSKPGDIWNRCEFQCKLCSSIYIDRRTIRAHIQNQHKIHYKDYISDYGEPELPTPKWICKICGAETRHARNNIYTHLRECHSLTIDQYAIE-NGIPGAKVKVKSITWSGTARTA--------------------KTERWNKCIFKCPLCEKESTEKKNIRQHVQVEHDAVLEDLEQQHGDCELHTEYFFCQICSAEVKHCYKNIQMHLQRSHELNIAAYESK---------------------------FL-------------GKTISKKMGRGRIAKNDGSTVPCEKCGKKFSSNSNKQRHKRDYCAFAVG----------------------AEEDAQEELGLN------RCPVSGCGMKFERSLQMKKHLGEVHKINQNVSVKLREA--DRQAAE------IKQEPEQVTPSFLQEDSEEEKDIIP 1081          

HSP 2 Score: 145.976 bits (367), Expect = 2.558e-31
Identity = 73/186 (39.25%), Postives = 111/186 (59.68%), Query Frame = 0

Query:  342 FSSTFTCFF-CNEQFRKDYKLKLHLMLNHKSENAEDMIKA----KEELTKSKLDGCVHKCALCGSKYNSVANFTRHIKDVHNMSRAQYREDYGSSEVVSRMFKCELCEKEVKHTRNIIGAHMKMVHLISWREYQEILVKIRQGESVGDLPAPELFDCVICGVSVKYKREHLNKKHQITEDVYDELI 522
             S+   C+F C E F+KDY+L LHL L H+ E+ +++ KA     EE+  ++    +  CALC  ++N    F  HI++ H++S   Y+E YG  E  S+ F+C +C K +K+ RN I  H+K VH I+W +Y E + K R+G S   LP  +  +C +C ++VKY R+HL   H+ITE  Y+EL 
Sbjct:  272 LSTDLQCYFNCQEVFKKDYQLFLHLKLKHRLEDPDELKKAYAAANEEIALTRRSASLFNCALCPREFNDNGAFYGHIQNKHDISYKDYKEKYGRCETESKPFECNICSKVIKYDRNTIHTHLKNVHGINWTKYLERIRKTRRGVSPDPLPTLKTVECRVCNINVKYLRDHLRNAHKITEQEYEELF 457          

BLAST of hypothetical protein HMPREF1544_09400 vs. nr
Match: gi|1325276322|ref|XP_023322849.1| (uncharacterized protein LOC111697178 [Eurytemora affinis])

HSP 1 Score: 142.124 bits (357), Expect = 7.746e-30
Identity = 234/1044 (22.41%), Postives = 408/1044 (39.08%), Query Frame = 0

Query:  379 KAKEELTKSKLDGCVHKCALCGSKYNSVANFTRHIKDVHNMSRAQYRE-------DYGSSEVVSR-MFKCELCEKEVKHTRNIIGAHMKMVHLISWREYQEI--LVKIRQGES--VGD-----LPAPELFDCVICGVSVKYKREHLNKKHQITEDVYDELILKKNRGEDISDSLPARELVR--CLICDRECL--DFKKHIERSHQITEEMYEEIITSRGLEDQRRSISQFEESLVTNGKAKFSSKSSPGKSNKRSCSDSFGTSSVRSESPGTL-QVAGANPLSTDLQCYFGCDEVFKKDYQLYLHLKLKHRSETTDEIARAYEAADEEIALTKRSGSVFQCSLCPKQFNDNGAFYSHIQSKHDMQWRD-YKEQYGRCEVESSPFECKICSRVIKYDRNTIHTHLKNVHGINW----TLYLDRL--RKMRRGEQ-PDALPQIEMIE--------CRVCNVQVKY----LKDHLRNAHKITEQEYESLFEGEHDLFGSTTQAMELGPSH--VESGTSKVKDPITPQMVITDGVNLLSS---QPVAIPKPPKSDIQNKTNKACSSCEITFESRRGFIEHCTTVHS------MKFKTKSGITISAPTLQHPMKR------KLNSEPIVDLPKRIRRGTDTSGCG--------QSMGESNISSGSTDRHGGSSATQYTPNGVSKW-NQCVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHY----EKNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNSSVNLSKEDKW-NRCRFKCVLCKWT-NVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGYDSSTAQEVPFMTHGDDITSEPSNEQPIIVHSEALVDNGNQPSRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKY 1348
            +A+  L +      V +C +C    + V +   HI+D H MS+  Y +        + SS   S+  + C++CE++ ++T   I  H++ +H I  ++Y++   + K  + ES  V D     LP  E+    +    V  K+E      +I ED    ++L  +RG   S   P  E ++  C  CD       F KHI ++H++  ++Y+ +     +  +R+ I +     +++  +  S   + G+ N            ++ + P  L +     P +T           F  DY     L L   S     +A  ++       L      +  C +C +++   G+F  H+  +HD   R  Y E +   ++    + CKIC   IK+ R+ I  HL+  H         LY+D    +    G+Q P+ +P    +E        C++CNV V      +++HL+  H ++  EY+         F ++       P+    +   S    P+    +I     LL     +P A P  P     NK    C  C   F S  GF  H +  HS      ++   K+GI I     +   K+       +N+    D    ++      G          Q+  +  I   +   H  S +  +  +  +KW N C Y+C  CG+   S + ++ H+ ++H +    Y  + +    +  R ++C++C     +    I+ H+K +HN+ ++ Y    E    Q   +       V   +P     PP      PS    L                KW N+C + C +C  T + +S     HIA  H +  + Y + +G    V V   C+LCN ++      +  H K  H + L+EY+ R             FM   DD +S      P   H             +NK  ++C IC+  +      + HI K H +   DY   +G+  +H   F C +C A +  N   I+ HL N H  +  +YE  Y
Sbjct:  874 EAEHALQQEAESASVVQCGICD---DMVKSLMTHIEDYHKMSKEAYLKICVGDDKKFSSSPAKSKPKWSCQICEEKAENTEKSIKEHLE-IHAIDLKDYEKTFDIPKGNENESKEVEDDDEIVLPDSEIMMEEVEDEDVNIKKEVDTNGDEINED---NVVL--HRG---SPETPWFESIKMKCKECDVYFWLGAFMKHIIQAHRLPIKVYK-VKYPETVIPKRQYICRICGVSISHYASPISGHLT-GRHNITMTDYYKKYEKLKVKPPSPLPEPEIVIPSNT-----------FPDDY-----LSLDSTSSKAQRMAEYHKTG----KLPWFESKITICRICNEEWL-LGSFRRHLYDRHDKISRTTYSEMFPDSDISIPTWRCKICGNGIKWVRDNIFYHLR-THNFTMEGYRQLYIDGSDDKTTALGKQMPNLVPISNSLETKEEKQFRCKLCNVLVALESTAIRNHLKTFHNMSINEYQP--------FKNSPNPTGFSPAFPLADPNNSAYNIPLYNNPMIQKPPPLLKKILPRPSAPPAQPGLPWYNKCKWTCMLCNKAFSS--GFWRHVSENHSYTKENYLQEFGKNGIDIVHYFCKICNKKIPWSGASINAHTKADHAMSLKEYEGLYGPKSDELVPDPQAEPDIPILPINMFPHLPSGSQIHYVHSPNKWFNGCEYKCQVCGRILHSVAGLSMHLKDTHMMDKFSYF-RQFGRAGINIRKYKCKICFKSFPWSGVSISKHVKQAHNISLQRYSNMYENGSTQESIYQEELANGVPGMDPIRENQPP------PSRDKGL----------------KWYNKCSWTCQICGTTISTNSSAFYKHIAQDHGVPIDEYKEKHGNTGFVYVDHTCKLCNKKVPFNGLAMCKHFKHTHNMQLEEYQAR-------------FMI-DDDGSSTGEYYAPTDEH------------WYNKGIWKCQICKNKNKSLGSYKKHIQKMHNMHYEDYFDKFGNAGIHEVVFRCKMCAATMSCNGVTIASHLLNNHKFTLAEYEKTY 1822          

HSP 2 Score: 135.191 bits (339), Expect = 8.086e-28
Identity = 216/1048 (20.61%), Postives = 368/1048 (35.11%), Query Frame = 0

Query:  395 KCALCGSKYNSVANFTRHIKDVHNMSRAQYREDYGSSEVVSRMFKCELCEKEVKHTRNIIGAHMKMVHLIS----WREYQEILVK---------------------------------IRQGESVGDLPAPE--LFDCVICGVS--VKYKREHLNKKH-QITEDVYDELILKKNRGEDISDSLPARELVRCLICDRECLDFKKHI---ERSHQITEEMYEEIIT------SRGLEDQRRSISQFEESLVTNGKAKFSSKSSPGKSNKRSCSDSFGTSSVRSESPGTLQVAGANPLSTDLQCYFGCDEVFKKDYQ--LYLHLKLKHRSETTDEIARAYEAADEEIALTKRSGSVFQCSLCPKQFNDNGAFYSHIQSKHDMQWRDYKEQYGRCEVESSPFECKICSRVIKYDRNTIHTHLKNVHGINWTLYLDRLRKMRRGEQPDALPQIEMIECRVCNVQVKY----LKDHLRNAHKITEQEYESLFEGEHDLFGSTTQAMELGPSHVESGTSKVKDPITPQMVITDGVNLLSSQPVAIPKPPKSDIQNKTNKACSSCEITFESRRGFIEHCTTVHSM-KFKT-----KSGITISAPTLQHPMKRKLNSEPI--VDLPKRIRRGTDTS-----------GCGQSMGESNISSGSTDRHGGSSATQYTP-----NGVSKWNQCVYQCCFCGKSTQSRSS-MTSHIHNSHGIPIKEYKEK--NYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNSSVNLSKEDKWNRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGYDSSTAQEVP---------FMTHGDDITSEPSNEQPIIVHSEALVDNGNQPSRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYG-DCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKY 1348
            KC  C   Y  +  F +HI   H +    Y+  Y  + +  R + C +C   + H  + I  H+   H I+    +++Y+++ VK                                 + +    G LP  E  +  C IC     +   R HL  +H +I+   Y E+        D   S+P     RC IC       + +I    R+H  T E Y ++        +  L  Q  ++     SL T  + +F  K         S +      +  + S    Q    +P  T     F   +     Y   LY +  ++       +I     A   +  L   +   + C LC K F+    F+ H+   H     +Y +++G+                               +GI+   Y                       C++CN ++ +    +  H +  H ++ +EYE L+                GP   E       +P  P + I    +L S   +     P     N     C  C     S  G   H    H M KF       ++GI I     ++  K    S P   V + K +++  + S              +S+ +  +++G     G     +  P      G+  +N+C + C  CG +  + SS    HI   HG+PI EYKEK  N   + V+     C+LC+ +  F    +  H K +HNM++E Y+   M  +D                                    G  +      + E  +N+  +KC +CK  N      + HI   H+M+YE Y    G A +  V F+C++C + M      I +H+ + HK TL EYE           +E           F+      +SEP+   P++   E L    ++P  + KC++ C IC         +R+H    H +    Y   YG D     E + C +C+  +K   ++++ H++N H +   +Y  +Y
Sbjct: 1044 KCKECDV-YFWLGAFMKHIIQAHRLPIKVYKVKYPETVIPKRQYICRICGVSISHYASPISGHLTGRHNITMTDYYKKYEKLKVKPPSPLPEPEIVIPSNTFPDDYLSLDSTSSKAQRMAEYHKTGKLPWFESKITICRICNEEWLLGSFRRHLYDRHDKISRTTYSEMF------PDSDISIPTW---RCKICGNGIKWVRDNIFYHLRTHNFTMEGYRQLYIDGSDDKTTALGKQMPNLVPISNSLETKEEKQFRCKLCNVLVALESTAIRNHLKTFHNMSINEYQPFKNSPNPTGFSPAFPLADPNNSAYNIPLYNNPMIQKPPPLLKKILPRPSAPPAQPGLPWYNKCKWTCMLCNKAFS--SGFWRHVSENHSYTKENYLQEFGK-------------------------------NGIDIVHYF----------------------CKICNKKIPWSGASINAHTKADHAMSLKEYEGLY----------------GPKSDELVPDPQAEPDIPILPINMFPHLPSGSQIHYVHSPNKWF-NGCEYKCQVCGRILHSVAGLSMHLKDTHMMDKFSYFRQFGRAGINIR----KYKCKICFKSFPWSGVSISKHVKQAHNISLQRYSNMYENGSTQESIYQEELANGVP---GMDPIRENQPPPSRDKGLKWYNKCSWTCQICGTTISTNSSAFYKHIAQDHGVPIDEYKEKHGNTGFVYVD---HTCKLCNKKVPFNGLAMCKHFKHTHNMQLEEYQARFMIDDD------------------------------------GSSTGEYYAPTDEHWYNKGIWKCQICKNKNKSLGSYKKHIQKMHNMHYEDYFDKFGNAGIHEVVFRCKMCAATMSCNGVTIASHLLNNHKFTLAEYEKTYLKDGEDEGEEGIEVQPQDGEHFLAGMKATSSEPA---PVLSKEEML--RKDKP-WYQKCKYFCQICNNPYFSISALRNHTKVKHNMDRDTYVGIYGLDAGRVIEDYSCKICNRTLKCEGRSLNAHMRNCHKMGIVEYSKRY 1957          

HSP 3 Score: 128.257 bits (321), Expect = 1.086e-25
Identity = 216/1020 (21.18%), Postives = 383/1020 (37.55%), Query Frame = 0

Query:  362 KLHLMLNHKSENAEDMIKAKEELTKSKL-------DGCVHKCALCGSKYNSVANFTRHIKDVHNMSRAQYREDYGSSEVVSRMFKCELCEKEVKHTRNIIGAHMKMVHLISWREYQEILV-----KIRQGESVGDLPAPE--LFDCVICGVSVKYK--REHLNKKHQITEDVYDELILKKNRGEDISDSLPARELVRCLICDRECLDFK----KHIERSHQITEEMYEEIITSRGLEDQRRSISQFEESLVTNGKAKFSSKSSPGKSNKRSCSDSFGTSSVRSESPGTLQVAGANPLSTDLQCYFGCDEVFKKDYQLYLHLKLKHRSETTDEIARAYEAADEEIALTKRSGSVFQCSLCPKQFNDNGAFYSHIQSKHD-MQWRDYKEQYGRCEVESSPFECKICSRVIKYDRNTIHTHLKNVHGIN-----WTLYLDRLRKM------------------------RRGEQPDALPQ----IEMIECRVCNVQVKYLKDHLRNAHKITEQEYESLFEGEHDLFGSTTQAMELGPSHVESGTSKVKDPITPQMVITDGVNLLSSQPVAIPKPPKSDIQNKTNKACSSCEITFESRRGFIEHCTTVHSMKFKTKSGITISAPTLQHPMKRKLNSEPIVDLPK-------------RIRRGTDTSGCGQSMGESNISSGSTDRHGGSSATQYTPNGVSKWNQC-VYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKLVASP---PAQSVAKPSCSTP---LLVGKLSNSSVNLSKEDKWNRCRF---KCVLCKWTNVDSR--QMRAHIATKH-HMNYETYVQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGYDSSTAQEVPFMTHGDDITSEPSNEQPIIVHSEALVDNGNQPSRWNKCRFQCAICQKLSS-EKRHIRDHIVKAHGVAMPDYE 1300
            ++  +LN  +E     ++  EEL  S         +   H C +C  K+ ++  F+ H+ D H MS   YRE + S ++  +++ C LCEKE+K T++ I  H+K +H +S +EY   ++     K++Q      LP  E     C IC     +   ++HL  KH  +   Y  L  + +   + S   P   + +C+IC  +    K    +HI R H ++ E Y     +  +ED ++     +   +   +   + K  P +         F +  VR E   T  +  + P         G DE   K +  +   KL H+                        G V  C L         +F +HI  KH  M  + Y +++   ++E   + C +C   IK+ +  I  HLK +H +       T  +    K+                           E   AL Q      +++C +C+  VK L  H+ + HK++++ Y  +  G+   F S+                                            P KS    K   +C  CE   E+    I+    +H++  K     T   P       +++  +  + LP               I++  DT+  G  + E N+       H GS  T +  +   K  +C VY   + G       +   HI  +H +PIK YK K YP+  +  R + CR+C          I+ HL   HN+ +  Y K                 K E   V  P   P   +  PS + P   L +   S+ +  +++  K  +  +   K  +C+  N +      R H+  +H  ++  TY +    +++    ++C++C + +K  R NI+ H++  H  T++ Y                ++   DD T+    + P       LV   N      + +F+C +C  L + E   IR+H+   H +++ +Y+
Sbjct:  455 RVSYLLNPTTEEDVSALQITEELELSNFMVSLPWYEARYHHCLIC-EKFYTLGTFSSHVLDAHGMSMVLYREKFPSDDLSVKLWNCSLCEKELKWTQSNISKHLKSMHRLSLQEYSLTILGPDGFKVKQVRLEPSLPWYESRTHRCRICNHVFIFSELKKHLRLKHNCSFSNYKLLFPQSSLSAEASSEDP---VWKCMICSAKTAWTKEPIIQHI-RKHNLSLESY----AAEHVEDAKQDEDVDDTQPLPPVECVLNVKDEPEEE-----KSDFNSRIVRLEPRDTTPMKKSMPRE---MVKVGSDE---KLWVPWFESKL-HKCLIC--------------------GEVRTCGL---------SFKNHIYDKHQYMSRKQYVKRFPGVDIEPDAWNCPLCHNTIKWTKKCIVDHLKTIHMLTREEFELTFNIPSRHKVDAPEGGEFNFNARTELGIANKEVAEAQEAEHALQQEAESASVVQCGICDDMVKSLMTHIEDYHKMSKEAYLKICVGDDKKFSSS--------------------------------------------PAKS----KPKWSCQICEEKAENTEKSIKEHLEIHAIDLKDYEK-TFDIPKGNENESKEVEDDDEIVLPDSEIMMEEVEDEDVNIKKEVDTN--GDEINEDNVV-----LHRGSPETPWFESIKMKCKECDVY--FWLG-------AFMKHIIQAHRLPIKVYKVK-YPETVIPKRQYICRICGVSISHYASPISGHLTGRHNITMTDYYK-----------------KYEKLKVKPPSPLPEPEIVIPSNTFPDDYLSLDSTSSKAQRMAEYHKTGKLPWFESKITICRICNEEWLLGSFRRHLYDRHDKISRTTYSEMFPDSDISIPTWRCKICGNGIKWVRDNIFYHLR-THNFTMEGYRQL-------------YIDGSDDKTTALGKQMP------NLVPISNSLETKEEKQFRCKLCNVLVALESTAIRNHLKTFHNMSINEYQ 1321          

HSP 4 Score: 100.908 bits (250), Expect = 2.616e-17
Identity = 84/341 (24.63%), Postives = 142/341 (41.64%), Query Frame = 0

Query: 1037 ETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYE--KNCMQPEDW--------PTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNSSVNLSKED--KWNRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGYDSSTAQEVPFMTHGDDITSEPSNEQPIIVHSEALVDNGNQPSRW-NKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKYGRVPDEPAIPLQPAAN 1364
            E + F+C+LC+         I  HLK  HNM I  Y+  KN   P  +        P  S   +      ++  PP           PLL   L   S   ++     +N+C++ C+LC      S     H++  H    E Y+Q  G   +  V + C++CN ++     +I AH K  H ++L EYE   G Y   + + VP      DI   P N  P +  S + +   + P++W N C ++C +C ++      +  H+   H +    Y   +G   ++   + C +C      +  +IS H++  H +S Q Y + Y     + +I  +  AN
Sbjct: 1285 EEKQFRCKLCNVLVALESTAIRNHLKTFHNMSINEYQPFKNSPNPTGFSPAFPLADPNNSAYNIPLYNNPMIQKPP-----------PLLKKILPRPSAPPAQPGLPWYNKCKWTCMLC--NKAFSSGFWRHVSENHSYTKENYLQEFGKNGIDIVHYFCKICNKKIPWSGASINAHTKADHAMSLKEYE---GLYGPKSDELVPDPQAEPDIPILPINMFPHLP-SGSQIHYVHSPNKWFNGCEYKCQVCGRILHSVAGLSMHLKDTHMMDKFSYFRQFGRAGINIRKYKCKICFKSFPWSGVSISKHVKQAHNISLQRYSNMYENGSTQESIYQEELAN 1608          

BLAST of hypothetical protein HMPREF1544_09400 vs. nr
Match: gi|1325281336|ref|XP_023325348.1| (uncharacterized protein LOC111699037 isoform X2 [Eurytemora affinis])

HSP 1 Score: 108.997 bits (271), Expect = 7.237e-20
Identity = 145/709 (20.45%), Postives = 288/709 (40.62%), Query Frame = 0

Query:  672 DEIARAYEAADEEIALTKRSGSVFQCSLCPKQFNDNGAFYS---HIQSKHDMQWRDYKEQYGRCEVESSPFECKICSRVIKYDRNTIHTHLKNVHGINWTLYLDRLR----------KMRRGEQ----PDALPQIEMIECRVCNVQVK--YLKDHLRNAHKITEQEYESLFEGEHDLFGSTTQAMELGPSHVESGTSKVKDPITP--QMVITDGVNLLSSQPVAIPKPPKSDIQNKTNKACSSCEITFESRRGFIEHCTTVHSMKFKTKSGITISAPTLQHPMKRKLNSEPIVDLPKRIRRGTDTSGCGQSMGESN-ISSGSTDRHGGSSATQYTPNGVSKWNQCVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWF-QCRLCSARTKFVKDCIAPHLKMSHNMEIEHYE-KNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNSSVNLSKEDKW--------NRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKF-QCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGY-DSSTAQ-EVPFMTHGDDITSEPSNEQPIIVHSEALVDNGNQPSRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYG-DCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDY 1344
            D I    +  D E   T+R   + QC LC K  N    F S   H++ +H M  + YKE++G      +   CK+C +VIK   + ++ H+  VH +++  Y  +            K + G       D + ++ + +C +C ++++   ++ H+++ HKI   EY   + GE +    T    +L    +     K+   +     + I+  +NL  S      +   ++I   +   C  C ++F S + F  H  + HS+                                       +T    ++ G  N     S+D  G               + C ++C  C    Q++   + H+   H + +K Y+ + +  +  E + F +C+ C     F    I  HL+  H+M ++ Y+ K  +  E     S  LV ++          +   K +    LL  K   S   L K  +           C++ C++CK   +    ++ H+  +H ++ + Y++  G  + V + + +C++C+SE+   R+++  H+K  H +T  +Y+    G+ D  TA+ E+P         S  ++ +P I  S+A         R   C++ C  C  + + ++ I+ H++K H +   DY + +G D  +   Y  C +C   +      +  H ++ H ++ ++Y
Sbjct:  431 DAILDTEDVPDHEKVFTERIEEMCQC-LC-KICNTTMKFRSLREHVRFQHSMNIQMYKEKHGNIYTRLTYHRCKMCDKVIKASYDFLNNHVSKVHKMSFAQYRTKFEIFKGRSKKIFKRKNGGMVKVFTDRVEEMCLCKCALCELELEPYNMRHHVKSKHKIALDEYRDTY-GEIEFTRETFHRCKLCQKEILFEYQKLSSHLYSGHSLGISKYINLFLSSSDNSTENENTEIS--SEHECRICCVSFPSNK-FRYHLQSEHSL---------------------------------------NTRKYEETFGHINDFQYRSSDDVG---------------DMCSFKCKICDIVLQAQD-FSVHLSTEHSLNLKAYRGE-HGKLTFENKTFHRCKECEKDILFTIAAIKRHLETKHSMTLKKYKMKYNIFTELKDKVSSVLVKQT---------GKYQDKNNVERFLLKLKEEGSGEELLKAPEGAIFANSIAEMCKYSCLMCK-EELTLLAIKKHLPKEHGLSLQKYIRQFGVLQPVQLVYHRCKVCSSELIFNREDVTGHLKRAHSLTYGQYKSFFNGFSDVITAKPELPLPRRSRRNNSLSADLEPEIFTSDA---------RIPVCQYACKECNSVFNRRQLIK-HVIKVHKLTYKDYRSKHGSDTPLVKIYHSCKICSTRLLACYDTLKSHARDKHNITFREY 1057          

BLAST of hypothetical protein HMPREF1544_09400 vs. nr
Match: gi|1325284048|ref|XP_023326721.1| (uncharacterized protein LOC111700133 isoform X1 [Eurytemora affinis])

HSP 1 Score: 108.997 bits (271), Expect = 7.669e-20
Identity = 92/393 (23.41%), Postives = 164/393 (41.73%), Query Frame = 0

Query:  990 KWNQ-CVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNSSVNLSKEDKWNR-CRFKCVL--CKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYE-DRVGGYDSSTAQEVPFMTHGDDITSEPSNEQP----------------------IIVH------SEALVDNGNQPSRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYG-DCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKY 1348
            +W + C Y C  C K+     S   H+   H + +++Y  +   + E   R++ C++C+    +    I  HL+  HN+ +E Y                  +K EP   +     S AK + +   +V K  N    L  E+ W   C + C +  C      S     HI + H ++ +TY +  GT   V  +++C +CN+ + H   NI +H+  +H +++ EYE D +   D+    E+  +   D  +      +                       II+        E + D  ++ + ++     C IC K  +  +    H  K HG+    Y   YG D E++ +Y  C +C+++++    +I  HL+ VHG + QDYE KY
Sbjct:  478 RWYEGCSYVCIPCNKNYDIYFSYHGHVKKIHNLSMEQYHAEYGQEGET-NRYYSCKICNTNVVWRMFNIRKHLESQHNLSMEDYS-----------------AKYEPDSRSKTQDFSKAKSAGNNLSIVQKTENKI--LDDEEVWYEGCLYTCQMLGCNKMFNLSESYHKHITSFHQIDLQTYHKKFGTKGEVYKQYECLICNARIAHKYNNIASHITLIHDLSMSEYEQDYIPYEDTIATGEMDIIEDQDQESELIIESEEGEDGNEGEEGNEEERYEFTEEDIIIEETPPEVKEPITDGASEHAWYHGSDLTCQICFKNFANDQWYFHHFSKIHGITKEQYNEKYGKDAEIYHQYQ-CLICNSKIRWKYSSIRQHLKQVHGTNIQDYEVKY 849          

HSP 2 Score: 102.064 bits (253), Expect = 1.063e-17
Identity = 106/429 (24.71%), Postives = 172/429 (40.09%), Query Frame = 0

Query:  998 CCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVA--------------KPSCSTPLLVGKLSN------SSVNLSK----------EDKW-NRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDR--VGGYDSSTAQEVPFMTHGDDITSEPSNEQPIIVHSE-----------------------ALVDNGNQPS-------------RW-NKCRFQCAIC--QKLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKYGRVPDE 1354
            C  C K+  +      H    HGI  ++Y EK   D E+  + +QC +C+++ ++    I  HLK  H   I+ YE   M  E    T   L +  E    A  P   V+                +    LL G++S+      S   LSK          E +W +     C++C  + VD      HI T H ++ E Y    GT       ++C +C+  +K   Q+I  H+K  H+I++ +YE +  V    S+ + ++  +T    +        P  +  E                        LV N  +P              +W N+C+F C  C  +K  +  + +R H+   H +   +YE+ YG+ E  T+Y+ C +C+ E+K     I  HL NVH L   DYE +Y    D+
Sbjct:  772 CQICFKNFANDQWYFHHFSKIHGITKEQYNEKYGKDAEIYHQ-YQCLICNSKIRWKYSSIRQHLKQVHGTNIQDYEVKYMDSEPELLTIDPLSALREQ---ARNPCHDVSSIKPVPSSLPPPPPLKASPAALLAGRVSSTQGAMFSPPTLSKAGSLTFESRPETQWYHGSDLTCLICNKSFVDFTYYCTHIRTVHQISKERYNDKYGTGAEAFRLYRCLVCHISIKWTYQSICGHLKS-HEISIKDYELKYPVLENQSTESGKISDLTMNVRVGGGEGEGSPKKMKLEDVPPVLPTLSDMMGSPRQFDLNSMLVPNPQKPKPSRISADISSPTVKWYNQCKFTCKACPEEKYFTVPQALRMHLNGIHSLTRKEYESRYGNAETVTKYWTCLMCNKELKCQKGIIIQHLLNVHTLELVDYERQYMDAGDQ 1195          

HSP 3 Score: 91.2781 bits (225), Expect = 1.925e-14
Identity = 92/362 (25.41%), Postives = 152/362 (41.99%), Query Frame = 0

Query:  994 CVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNSSVNLSKEDK--WNR-CRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCEL--CNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGYDSSTAQEVPFMTHGDDITSEPSNEQPIIVHSEALVDNGNQPSRWNKCRFQ-CAICQ-KLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKY 1348
            C Y C  C K+     +   H+ + H +   +Y E  Y   E+ETR F C LC+   K  +D I  HL+  H+M +E YE+    P          + +S     AS    S    +   P+  G  +    + S + +  W R   F C+ C           +HI +KH MN   Y +  G+ E +  ++ C +  C   +      I  H+K  H +T+ EYE +      +  + +P     D+I +EP      +   E +VD       W+    + CA+C+  +  +      HI ++H +++  Y   +G  E   ++  C VC  +V+     ++ HLQ  H L PQ Y  KY
Sbjct: 1303 CRYSCQICNKNWHYPQACRYHLDSVHKLSRAQY-ETEYGSCEIETRIFNCSLCTRTMKCERDIIKYHLEAKHSMSLEEYEEQVPNP-------VVTIGESRRSNSASHSRTSSLTGTQFIPISEGSPAKPGADGSSDPRIPWYRGSNFTCIPCNRQFTAQVSYISHINSKHGMNLREYREQFGSQEEIK-QYNCRVPGCTRFVSWTYSGINDHLKS-HSLTMGEYEQQY----LNQLEHLP----KDEINAEP------VESLEKMVD------MWSVGESRICAVCETTIFGDVGKFNTHITESHAISLDQYTTLHGSSEKKKKH-CCLVCGEDVELLPGILAAHLQ-THSLFPQQYYHKY 1632          

HSP 4 Score: 72.0182 bits (175), Expect = 1.329e-8
Identity = 36/110 (32.73%), Postives = 58/110 (52.73%), Query Frame = 0

Query:  368 NHKSENAEDMIKAKEELTKSKLD------GCVHKCALCGSKYNSVANFTRHIKDVHNMSRAQYREDYGSSEVVSRMFKCELCEKEVKHTRNIIGAHMKMVHLISWREYQE 471
            N +  N +D + +   L    +D      GC + C +C   ++       H+  VH +SRAQY  +YGS E+ +R+F C LC + +K  R+II  H++  H +S  EY+E
Sbjct: 1273 NPQHSNLDDSVTSMSSLEVEGMDYVPWYFGCRYSCQICNKNWHYPQACRYHLDSVHKLSRAQYETEYGSCEIETRIFNCSLCTRTMKCERDIIKYHLEAKHSMSLEEYEE 1382          

BLAST of hypothetical protein HMPREF1544_09400 vs. nr
Match: gi|1325284050|ref|XP_023326722.1| (uncharacterized protein LOC111700133 isoform X2 [Eurytemora affinis])

HSP 1 Score: 108.612 bits (270), Expect = 9.133e-20
Identity = 92/393 (23.41%), Postives = 164/393 (41.73%), Query Frame = 0

Query:  990 KWNQ-CVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNSSVNLSKEDKWNR-CRFKCVL--CKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYE-DRVGGYDSSTAQEVPFMTHGDDITSEPSNEQP----------------------IIVH------SEALVDNGNQPSRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYG-DCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKY 1348
            +W + C Y C  C K+     S   H+   H + +++Y  +   + E   R++ C++C+    +    I  HL+  HN+ +E Y                  +K EP   +     S AK + +   +V K  N    L  E+ W   C + C +  C      S     HI + H ++ +TY +  GT   V  +++C +CN+ + H   NI +H+  +H +++ EYE D +   D+    E+  +   D  +      +                       II+        E + D  ++ + ++     C IC K  +  +    H  K HG+    Y   YG D E++ +Y  C +C+++++    +I  HL+ VHG + QDYE KY
Sbjct:  465 RWYEGCSYVCIPCNKNYDIYFSYHGHVKKIHNLSMEQYHAEYGQEGET-NRYYSCKICNTNVVWRMFNIRKHLESQHNLSMEDYS-----------------AKYEPDSRSKTQDFSKAKSAGNNLSIVQKTENKI--LDDEEVWYEGCLYTCQMLGCNKMFNLSESYHKHITSFHQIDLQTYHKKFGTKGEVYKQYECLICNARIAHKYNNIASHITLIHDLSMSEYEQDYIPYEDTIATGEMDIIEDQDQESELIIESEEGEDGNEGEEGNEEERYEFTEEDIIIEETPPEVKEPITDGASEHAWYHGSDLTCQICFKNFANDQWYFHHFSKIHGITKEQYNEKYGKDAEIYHQYQ-CLICNSKIRWKYSSIRQHLKQVHGTNIQDYEVKY 836          

HSP 2 Score: 101.679 bits (252), Expect = 1.321e-17
Identity = 106/429 (24.71%), Postives = 172/429 (40.09%), Query Frame = 0

Query:  998 CCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVA--------------KPSCSTPLLVGKLSN------SSVNLSK----------EDKW-NRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDR--VGGYDSSTAQEVPFMTHGDDITSEPSNEQPIIVHSE-----------------------ALVDNGNQPS-------------RW-NKCRFQCAIC--QKLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKYGRVPDE 1354
            C  C K+  +      H    HGI  ++Y EK   D E+  + +QC +C+++ ++    I  HLK  H   I+ YE   M  E    T   L +  E    A  P   V+                +    LL G++S+      S   LSK          E +W +     C++C  + VD      HI T H ++ E Y    GT       ++C +C+  +K   Q+I  H+K  H+I++ +YE +  V    S+ + ++  +T    +        P  +  E                        LV N  +P              +W N+C+F C  C  +K  +  + +R H+   H +   +YE+ YG+ E  T+Y+ C +C+ E+K     I  HL NVH L   DYE +Y    D+
Sbjct:  759 CQICFKNFANDQWYFHHFSKIHGITKEQYNEKYGKDAEIYHQ-YQCLICNSKIRWKYSSIRQHLKQVHGTNIQDYEVKYMDSEPELLTIDPLSALREQ---ARNPCHDVSSIKPVPSSLPPPPPLKASPAALLAGRVSSTQGAMFSPPTLSKAGSLTFESRPETQWYHGSDLTCLICNKSFVDFTYYCTHIRTVHQISKERYNDKYGTGAEAFRLYRCLVCHISIKWTYQSICGHLKS-HEISIKDYELKYPVLENQSTESGKISDLTMNVRVGGGEGEGSPKKMKLEDVPPVLPTLSDMMGSPRQFDLNSMLVPNPQKPKPSRISADISSPTVKWYNQCKFTCKACPEEKYFTVPQALRMHLNGIHSLTRKEYESRYGNAETVTKYWTCLMCNKELKCQKGIIIQHLLNVHTLELVDYERQYMDAGDQ 1182          

HSP 3 Score: 90.8929 bits (224), Expect = 2.333e-14
Identity = 92/362 (25.41%), Postives = 152/362 (41.99%), Query Frame = 0

Query:  994 CVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNSSVNLSKEDK--WNR-CRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKFQCEL--CNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGYDSSTAQEVPFMTHGDDITSEPSNEQPIIVHSEALVDNGNQPSRWNKCRFQ-CAICQ-KLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKY 1348
            C Y C  C K+     +   H+ + H +   +Y E  Y   E+ETR F C LC+   K  +D I  HL+  H+M +E YE+    P          + +S     AS    S    +   P+  G  +    + S + +  W R   F C+ C           +HI +KH MN   Y +  G+ E +  ++ C +  C   +      I  H+K  H +T+ EYE +      +  + +P     D+I +EP      +   E +VD       W+    + CA+C+  +  +      HI ++H +++  Y   +G  E   ++  C VC  +V+     ++ HLQ  H L PQ Y  KY
Sbjct: 1290 CRYSCQICNKNWHYPQACRYHLDSVHKLSRAQY-ETEYGSCEIETRIFNCSLCTRTMKCERDIIKYHLEAKHSMSLEEYEEQVPNP-------VVTIGESRRSNSASHSRTSSLTGTQFIPISEGSPAKPGADGSSDPRIPWYRGSNFTCIPCNRQFTAQVSYISHINSKHGMNLREYREQFGSQEEIK-QYNCRVPGCTRFVSWTYSGINDHLKS-HSLTMGEYEQQY----LNQLEHLP----KDEINAEP------VESLEKMVD------MWSVGESRICAVCETTIFGDVGKFNTHITESHAISLDQYTTLHGSSEKKKKH-CCLVCGEDVELLPGILAAHLQ-THSLFPQQYYHKY 1619          

HSP 4 Score: 72.0182 bits (175), Expect = 1.373e-8
Identity = 36/110 (32.73%), Postives = 58/110 (52.73%), Query Frame = 0

Query:  368 NHKSENAEDMIKAKEELTKSKLD------GCVHKCALCGSKYNSVANFTRHIKDVHNMSRAQYREDYGSSEVVSRMFKCELCEKEVKHTRNIIGAHMKMVHLISWREYQE 471
            N +  N +D + +   L    +D      GC + C +C   ++       H+  VH +SRAQY  +YGS E+ +R+F C LC + +K  R+II  H++  H +S  EY+E
Sbjct: 1260 NPQHSNLDDSVTSMSSLEVEGMDYVPWYFGCRYSCQICNKNWHYPQACRYHLDSVHKLSRAQYETEYGSCEIETRIFNCSLCTRTMKCERDIIKYHLEAKHSMSLEEYEE 1369          

BLAST of hypothetical protein HMPREF1544_09400 vs. nr
Match: gi|1325281334|ref|XP_023325347.1| (uncharacterized protein LOC111699037 isoform X1 [Eurytemora affinis])

HSP 1 Score: 108.227 bits (269), Expect = 1.144e-19
Identity = 145/709 (20.45%), Postives = 288/709 (40.62%), Query Frame = 0

Query:  672 DEIARAYEAADEEIALTKRSGSVFQCSLCPKQFNDNGAFYS---HIQSKHDMQWRDYKEQYGRCEVESSPFECKICSRVIKYDRNTIHTHLKNVHGINWTLYLDRLR----------KMRRGEQ----PDALPQIEMIECRVCNVQVK--YLKDHLRNAHKITEQEYESLFEGEHDLFGSTTQAMELGPSHVESGTSKVKDPITP--QMVITDGVNLLSSQPVAIPKPPKSDIQNKTNKACSSCEITFESRRGFIEHCTTVHSMKFKTKSGITISAPTLQHPMKRKLNSEPIVDLPKRIRRGTDTSGCGQSMGESN-ISSGSTDRHGGSSATQYTPNGVSKWNQCVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWF-QCRLCSARTKFVKDCIAPHLKMSHNMEIEHYE-KNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNSSVNLSKEDKW--------NRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVVTVKF-QCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGY-DSSTAQ-EVPFMTHGDDITSEPSNEQPIIVHSEALVDNGNQPSRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYG-DCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDY 1344
            D I    +  D E   T+R   + QC LC K  N    F S   H++ +H M  + YKE++G      +   CK+C +VIK   + ++ H+  VH +++  Y  +            K + G       D + ++ + +C +C ++++   ++ H+++ HKI   EY   + GE +    T    +L    +     K+   +     + I+  +NL  S      +   ++I   +   C  C ++F S + F  H  + HS+                                       +T    ++ G  N     S+D  G               + C ++C  C    Q++   + H+   H + +K Y+ + +  +  E + F +C+ C     F    I  HL+  H+M ++ Y+ K  +  E     S  LV ++          +   K +    LL  K   S   L K  +           C++ C++CK   +    ++ H+  +H ++ + Y++  G  + V + + +C++C+SE+   R+++  H+K  H +T  +Y+    G+ D  TA+ E+P         S  ++ +P I  S+A         R   C++ C  C  + + ++ I+ H++K H +   DY + +G D  +   Y  C +C   +      +  H ++ H ++ ++Y
Sbjct:  463 DAILDTEDVPDHEKVFTERIEEMCQC-LC-KICNTTMKFRSLREHVRFQHSMNIQMYKEKHGNIYTRLTYHRCKMCDKVIKASYDFLNNHVSKVHKMSFAQYRTKFEIFKGRSKKIFKRKNGGMVKVFTDRVEEMCLCKCALCELELEPYNMRHHVKSKHKIALDEYRDTY-GEIEFTRETFHRCKLCQKEILFEYQKLSSHLYSGHSLGISKYINLFLSSSDNSTENENTEIS--SEHECRICCVSFPSNK-FRYHLQSEHSL---------------------------------------NTRKYEETFGHINDFQYRSSDDVG---------------DMCSFKCKICDIVLQAQD-FSVHLSTEHSLNLKAYRGE-HGKLTFENKTFHRCKECEKDILFTIAAIKRHLETKHSMTLKKYKMKYNIFTELKDKVSSVLVKQT---------GKYQDKNNVERFLLKLKEEGSGEELLKAPEGAIFANSIAEMCKYSCLMCK-EELTLLAIKKHLPKEHGLSLQKYIRQFGVLQPVQLVYHRCKVCSSELIFNREDVTGHLKRAHSLTYGQYKSFFNGFSDVITAKPELPLPRRSRRNNSLSADLEPEIFTSDA---------RIPVCQYACKECNSVFNRRQLIK-HVIKVHKLTYKDYRSKHGSDTPLVKIYHSCKICSTRLLACYDTLKSHARDKHNITFREY 1089          

BLAST of hypothetical protein HMPREF1544_09400 vs. nr
Match: gi|1325328631|ref|XP_023346169.1| (uncharacterized protein LOC111715135 [Eurytemora affinis])

HSP 1 Score: 92.8189 bits (229), Expect = 4.814e-17
Identity = 42/85 (49.41%), Postives = 59/85 (69.41%), Query Frame = 0

Query:   65 LSCFLCGGTHIYPGPRFENHLMNEHGAVFDLEFIIQLSLFKQEHGRLPHVEGEPAASNGHWENGNAPTPKSADTQDGFAQTETKP 149
            ++CFLCGGTHIYPGPRFE HL+NEHGA+FD+EF+I++SL+K +H  LP ++ +P       ENG        D++    QT+ +P
Sbjct:    8 ITCFLCGGTHIYPGPRFEAHLINEHGALFDIEFLIKVSLYKSQHSSLPELQIDPQPK----ENGIVTQIPRIDSE---TQTDQEP 85          

BLAST of hypothetical protein HMPREF1544_09400 vs. nr
Match: gi|1325273295|ref|XP_023321291.1| (uncharacterized protein LOC111696008 isoform X1 [Eurytemora affinis])

HSP 1 Score: 79.337 bits (194), Expect = 8.794e-11
Identity = 95/440 (21.59%), Postives = 166/440 (37.73%), Query Frame = 0

Query:  995 VYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNS------------SVNLSKEDKW-------NRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVV-TVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGYDSSTAQEVPFMTHGDDITSEP----------SNEQPIIVHSEAL---------VDNGNQP---------------------SRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKYGRVPDE-------PAIPLQPAANSDE 1367
             YQC  C  S Q R  +  HI N H + + EY+ K    I  E     C +C     +  D +  HLK SHNM + HY  + ++  D   + GT  S ++   ++S      ++ S S+  L+ + SN               N  K DK+       N   +KC +CK +  + R+   H+   H M +    +          V  +C++C  +       +  H++  H +T+ +Y D+    +    QEV   T  D+   E           + E  + ++  A+         +DN ++P                       ++ C ++C  C+  +  K     H+   H       +  +GDC   +    C VC  ++ +  +++S HL + H +    Y   +    D+       P I LQ A N  E
Sbjct:  524 TYQCQICTFSGQYRRFL-KHIANKHKLSVSEYRRKYGELIFDELVNHSCLVCHKSLVYTWDSVFTHLK-SHNMTLSHYNSSYLKRAD---SEGTRASNNDSSGMSSKD----SRKSFSSEDLIIENSNQHDQSCVRSESEEDSNDLKCDKFKYSDTLGNYSIYKCPICKLS-FEYRKFLQHLVKDHEMKFSVCKKKYSDDMFADKVMHKCKICGKDFVFAEDLLLCHLRK-HGLTIKQYSDQYLTDELKEVQEVKVDTESDEAKDETILGNNCDNGLTVENYLEINKAAVESEPTKINQLDNSSRPVGKSIVPYTEQKKCSKIRYSDKPYDYCTYKCPFCEICNLNKSSFSSHLTSKHKKKYS--QLMFGDCMFESVRHICKVCKEDISYEYRDLSQHLLDSHKMHIFIYLKTFLTKTDDEHVSTVIPEIHLQNAENQHE 950          

HSP 2 Score: 68.9366 bits (167), Expect = 1.129e-7
Identity = 102/457 (22.32%), Postives = 183/457 (40.04%), Query Frame = 0

Query:  965 SNISSGSTDRHGGSSATQYTPNGVSKWNQCVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWF-QCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQ-----------------PEDWPTTSGTLVSKSE-------PKLVASPPAQSVAKPSCSTPLLVGKLSNSSVN---------LSKEDKWNRCR-------------FKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVV---TVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYED-----------RVGGYDSS--TAQEVPFMTHGDDITSEPSNEQP-IIVHSEALVDNGN--------QPSRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFF-CAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKY 1348
            +NI+S +T++   + A +Y+ N     ++ V   C   K   +   +  H+  +H   +K Y EKNY  + +  + + QC +C     F K  +  HLK SHN +I  YE+  ++                 P + P+ + +  S           K +AS   + +  P  S      K+ N  V          ++  +   +CR             ++C +C ++    R+   HIA KH ++   Y +  G  E++    V   C +C+  + +   +++ H+K  H +TL  Y             R    DSS  ++++       +D+  E SN+     V SE+  D+ +          +  N   ++C IC KLS E R    H+VK H +     +  Y D     +    C +C  +       +  HL+  HGL+ + Y D+Y
Sbjct:  314 TNITSSNTNKQIKNGALKYS-NDFKNMSRTV---CLICKLELNHDILEFHLKTTHNTSLKVY-EKNYGTVNITKKIYHQCYICQNVLVFTKIFLREHLK-SHNTDIRTYEEELLKRSNDDKNVDHDEHDRTLPAESPSINQSFSSNVVSSSVRILAKSLASQKNKLLESPGISKK---RKIDNEEVENKRRKTMDYMNSNEMKEKCRVMRYSDVVNDFSTYQCQICTFSG-QYRRFLKHIANKHKLSVSEYRRKYG--ELIFDELVNHSCLVCHKSLVYTWDSVFTHLKS-HNMTLSHYNSSYLKRADSEGTRASNNDSSGMSSKDSRKSFSSEDLIIENSNQHDQSCVRSESEEDSNDLKCDKFKYSDTLGNYSIYKCPIC-KLSFEYRKFLQHLVKDHEMKFSVCKKKYSDDMFADKVMHKCKICGKDFVFAEDLLLCHLRK-HGLTIKQYSDQY 755          

BLAST of hypothetical protein HMPREF1544_09400 vs. nr
Match: gi|1325273297|ref|XP_023321292.1| (uncharacterized protein LOC111696008 isoform X2 [Eurytemora affinis])

HSP 1 Score: 78.9518 bits (193), Expect = 1.055e-10
Identity = 95/440 (21.59%), Postives = 166/440 (37.73%), Query Frame = 0

Query:  995 VYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWFQCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKLVASPPAQSVAKPSCSTPLLVGKLSNS------------SVNLSKEDKW-------NRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVV-TVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYEDRVGGYDSSTAQEVPFMTHGDDITSEP----------SNEQPIIVHSEAL---------VDNGNQP---------------------SRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFFCAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKYGRVPDE-------PAIPLQPAANSDE 1367
             YQC  C  S Q R  +  HI N H + + EY+ K    I  E     C +C     +  D +  HLK SHNM + HY  + ++  D   + GT  S ++   ++S      ++ S S+  L+ + SN               N  K DK+       N   +KC +CK +  + R+   H+   H M +    +          V  +C++C  +       +  H++  H +T+ +Y D+    +    QEV   T  D+   E           + E  + ++  A+         +DN ++P                       ++ C ++C  C+  +  K     H+   H       +  +GDC   +    C VC  ++ +  +++S HL + H +    Y   +    D+       P I LQ A N  E
Sbjct:  409 TYQCQICTFSGQYRRFL-KHIANKHKLSVSEYRRKYGELIFDELVNHSCLVCHKSLVYTWDSVFTHLK-SHNMTLSHYNSSYLKRAD---SEGTRASNNDSSGMSSKD----SRKSFSSEDLIIENSNQHDQSCVRSESEEDSNDLKCDKFKYSDTLGNYSIYKCPICKLS-FEYRKFLQHLVKDHEMKFSVCKKKYSDDMFADKVMHKCKICGKDFVFAEDLLLCHLRK-HGLTIKQYSDQYLTDELKEVQEVKVDTESDEAKDETILGNNCDNGLTVENYLEINKAAVESEPTKINQLDNSSRPVGKSIVPYTEQKKCSKIRYSDKPYDYCTYKCPFCEICNLNKSSFSSHLTSKHKKKYS--QLMFGDCMFESVRHICKVCKEDISYEYRDLSQHLLDSHKMHIFIYLKTFLTKTDDEHVSTVIPEIHLQNAENQHE 835          

HSP 2 Score: 68.5514 bits (166), Expect = 1.622e-7
Identity = 102/457 (22.32%), Postives = 183/457 (40.04%), Query Frame = 0

Query:  965 SNISSGSTDRHGGSSATQYTPNGVSKWNQCVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWF-QCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQ-----------------PEDWPTTSGTLVSKSE-------PKLVASPPAQSVAKPSCSTPLLVGKLSNSSVN---------LSKEDKWNRCR-------------FKCVLCKWTNVDSRQMRAHIATKHHMNYETYVQANGTAEVV---TVKFQCELCNSEMKHCRQNIYAHMKDVHKITLDEYED-----------RVGGYDSS--TAQEVPFMTHGDDITSEPSNEQP-IIVHSEALVDNGN--------QPSRWNKCRFQCAICQKLSSEKRHIRDHIVKAHGVAMPDYEATYGDCEVHTEYFF-CAVCHAEVKHNLKNISLHLQNVHGLSPQDYEDKY 1348
            +NI+S +T++   + A +Y+ N     ++ V   C   K   +   +  H+  +H   +K Y EKNY  + +  + + QC +C     F K  +  HLK SHN +I  YE+  ++                 P + P+ + +  S           K +AS   + +  P  S      K+ N  V          ++  +   +CR             ++C +C ++    R+   HIA KH ++   Y +  G  E++    V   C +C+  + +   +++ H+K  H +TL  Y             R    DSS  ++++       +D+  E SN+     V SE+  D+ +          +  N   ++C IC KLS E R    H+VK H +     +  Y D     +    C +C  +       +  HL+  HGL+ + Y D+Y
Sbjct:  199 TNITSSNTNKQIKNGALKYS-NDFKNMSRTV---CLICKLELNHDILEFHLKTTHNTSLKVY-EKNYGTVNITKKIYHQCYICQNVLVFTKIFLREHLK-SHNTDIRTYEEELLKRSNDDKNVDHDEHDRTLPAESPSINQSFSSNVVSSSVRILAKSLASQKNKLLESPGISKK---RKIDNEEVENKRRKTMDYMNSNEMKEKCRVMRYSDVVNDFSTYQCQICTFSG-QYRRFLKHIANKHKLSVSEYRRKYG--ELIFDELVNHSCLVCHKSLVYTWDSVFTHLKS-HNMTLSHYNSSYLKRADSEGTRASNNDSSGMSSKDSRKSFSSEDLIIENSNQHDQSCVRSESEEDSNDLKCDKFKYSDTLGNYSIYKCPIC-KLSFEYRKFLQHLVKDHEMKFSVCKKKYSDDMFADKVMHKCKICGKDFVFAEDLLLCHLRK-HGLTIKQYSDQY 640          

BLAST of hypothetical protein HMPREF1544_09400 vs. nr
Match: gi|758370470|emb|CEP07101.1| (hypothetical protein [Parasitella parasitica])

HSP 1 Score: 68.5514 bits (166), Expect = 1.193e-7
Identity = 115/551 (20.87%), Postives = 189/551 (34.30%), Query Frame = 0

Query:  697 CSLCPKQFNDNGAFYSHIQSKHDMQW--------RDYKEQYGRCEVESSPFECKICSRVIKYDRNTIHTHLKNVHGINWTLYLDRLRKMRRGEQPDALPQIEMIECRVCNVQVKYLKDHLRNAHKI---TEQEYESLFEGEHDLFGSTTQAMELGPSHVESGTSKVKDPITPQMVITDGVNLLSSQPVAIPKPPKSDIQNKTNKACSSCEITFESRRGFIEHCTTVHSMKFKTKSGITISAPTLQHPMKRKLNSEPIVDLPKRIRRGTDTSGCGQSMGESNISSGSTDRHGGSSATQYTPNGVSKWNQCVYQCCFCGKSTQSRSSMTSHIHNSHGIPIKEYKEKNYPDIEVETRWF----QCRLCSARTKFVKDCIAPHLKMSHNMEIEHYEKNCMQPEDWPTTSGTLVSKSEPKL------VASPPA---------QSVAKPSCSTPLLVGKLSNSSVNLSKEDKWNRCRFKCVLCKWTNVDSRQMRAHIATKHHMNYETY------VQANGTAEVVTVKFQCELCNSEMKHCRQNIYAHMKDVHKITLD 1211
            C  C  +F D   +  H+ SKH   W        R+     G  +++     C+ C    K      HTHL   H +N    LD +R  +   Q    P I  + C+     +     H ++AH +   + + ++   + + D      +A ++   +  +    +K P   +MV+   + +  + P   P P   D        C SC  TFESR  +  H   +H M  K+     I+ P        K   EP +D P           C ++ G  +I  G                                           H+ + H   +   +   +PD+ +E         CR C+   K  +D  A HL+  H+M++E   K C        T G L    +P        VA P A         +   KPS        +L  ++ N + E   +   F C+ C+    D    R H+  +H +  E        V A    +V    F C  C+   +  +    AH+K  HK+ L+
Sbjct:  168 CRACQAKFPDRNGYLYHLNSKH---WKELPPRALRNRTNMGGIPDMDDKDSHCRSCDAEYKRTPEAYHTHLVTKHKMN----LDSIRDFQANGQDIPNPHIYCLSCKTSFDTLALFVSHYKSAHYVRITSRRTFDPNIKLDPDDQALRCRACDIQYDNRTAYRGHLKGPH--RMVLESLLEISQATPGVEPDPADPDFH------CRSCNRTFESRGDYRIHLRAMHKMNLKS----LIAQP--------KEGVEPDLDDP-----NFHCRSCNRTYGTRHIYRG-------------------------------------------HLMSVHK--VAPLRTMTHPDMSIEPDVMDANNHCRSCNKTFKRNQDYRA-HLRSRHSMKLEPLVKLCT------PTPGVLPDPDDPNFFCRSCSVAYPNASDYRSHLRRRHALKPS-------RQLDVTTPNPNIEPDIHSTDFFCISCQKQFKDRGHYRGHLQYRHKIKLEKLASTKYNVNAAIIPDVDDPDFHCRSCDFTFED-KHKYRAHLKSTHKMKLN 626