BLAST of methionine synthase isoform 3 vs. L. salmonis genes
Match: EMLSAG00000001211 (supercontig:LSalAtl2s:LSalAtl2s11:1267259:1268974:-1 gene:EMLSAG00000001211 transcript:EMLSAT00000001211 description:"maker-LSalAtl2s11-augustus-gene-12.23")

HSP 1 Score: 56.9954 bits (136), Expect = 1.313e-8
Identity = 74/298 (24.83%), Postives = 123/298 (41.28%), Query Frame = 0

Query:   40 LTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDK----VYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGY-RAISFDELVTAFKLQVEALVEGGVDMLLVETV--IDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNC----------------ALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLD---------EGLLNIVGGCCGTSPAHIKAIAE 305
            L  P+ +  +H ++  AGADIV+  TF G+    E+ G + K       +N  +A++A++VA E            +G++       ++  V   P Y    S +E+   F+ QV   VE  +D +L E    I+ +      +AIE +   K+   P+  S  I  +    L G ++    +    A    +G+NC                AL   DL   + +M +    L    P+AG    F +  E P  +  +I    D         E  +  +GGCCG  P HI+A++E
Sbjct:   43 LENPEAVLQLHREFAAAGADIVQALTFYGSDDKLENRGNDAKNKFTGRSLNAKAAELAKKVAGE------------SGAL-------TLGGVCQCPSYLSGKSKEEVQEEFRKQVNVFVEEDLDFVLAEYFEHIEEME-----WAIEVL---KESNKPVAASMCIGPEG--DLHGVSSGECAVRMVKAGANVVGINCHFDPFICLEGMKLMKQALLEADLLDKVHLMVQP---LAYHTPDAG-KQGFIDLPEFPFALESRICTRFDMHRYAREAYELGIRYIGGCCGFEPYHIRALSE 307          

BLAST of methionine synthase isoform 3 vs. SwissProt
Match: gi|81622416|sp|Q9I2Q2.1|METH_PSEAE (RecName: Full=Methionine synthase; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase; AltName: Full=Methionine synthase, vitamin-B12 dependent; Short=MS)

HSP 1 Score: 712.605 bits (1838), Expect = 0.000e+0
Identity = 348/597 (58.29%), Postives = 436/597 (73.03%), Query Frame = 0

Query:  463 ATEALLEYAESVKGDGKKKE-RDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSK-EFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            ATEALL  A+  KG G  KE  D  WR   V+ R+ HALVKGIT +IVEDTEECR     PIEVIEGPLM GMNVVGDLFG+GKMFLPQVVKSARVMKQAVA+L PFIE  K  + +  GK+LMATVKGDVHDIGKNIVGVVLGCN YD+VD+GVMVP + I++ AI E  D+IGLSGLITPSLDEMV VA+EM++   ++PL+IGGATTS+ HTAVKI+  Y +   ++V DASR+V V   LL    K ++VA  + +Y  VR   A +  +   ++Y+ AI N+   DW   +  TP+F G +  +   L+ L  +IDWTPFF +W LAG+YPRILTDE+VGE ATSLF DAQ ML K+  EK + A+ V G WPAN    DD+ ++  +  T    T + LRQQT K    PN SLADF+APKE+G++DYIGGF+ TAG+G EE  K ++   DDY++I++KALADRLAEA AE +H++VR+E WGYA +E LDNE LIKE+Y GIRPAPGYPACPDHTEK TLF+ L+     GV LTE  AM+PAA+VSG+Y+AHPQ++YF +GKI+ DQV+  ++RKG E +  ++WL P
Sbjct:  634 ATEALLAIADDYKGGGAVKEAEDEEWRSYSVEKRLEHALVKGITTWIVEDTEECRQQCARPIEVIEGPLMSGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFIEAEKGDKPEAKGKILMATVKGDVHDIGKNIVGVVLGCNGYDVVDLGVMVPAEKILQTAIAEKCDIIGLSGLITPSLDEMVHVAKEMQRQNFQLPLMIGGATTSKAHTAVKIDPQYSNDAVVYVTDASRAVGVATSLLSKELKADYVARTRADYAVVRERTANRSARTERLSYEQAIANKPAFDWAGYQAPTPSFTGVRVLDEIDLAVLAEYIDWTPFFISWDLAGKYPRILTDEVVGEAATSLFNDAQAMLKKLIDEKLIKARAVFGFWPANQVEHDDLEVYGADGETLA--TLHHLRQQTIKPDGKPNLSLADFVAPKESGVRDYIGGFITTAGIGAEEVAKAYEAKGDDYNSIMVKALADRLAEACAEWLHERVRKEYWGYARDEHLDNEALIKEQYVGIRPAPGYPACPDHTEKGTLFELLDPQGLSGVSLTEHYAMFPAAAVSGWYFAHPQAQYFAVGKIDKDQVERYSQRKGQEASVSERWLAP 1228          

HSP 2 Score: 613.224 bits (1580), Expect = 0.000e+0
Identity = 298/488 (61.07%), Postives = 368/488 (75.41%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIAE-LAK--------------------------TNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSND 461
            LDG MGTMIQ YKL E D+R   F D    +KGNNDLL L+RPD+I+AI   Y +AGADI+ETNTF+ T ++Q DYG +   YE+N   A++AR+VAD  TA+ P+KPRFVAG +GPT++T SISP +N+PGYR ++FDELV  +      L+EGG D++L+ET+ DTLNAKAA+FA++ VF+E  +ELPIM+SGTITD SGRTLSGQTTEAF  S  HA   S+GLNCALGAK+LRPY++ ++ K+   VSAHPNAGLPN FGEYDE+P  MA  ++ F   G LNIVGGCCGT+PAHI+AIA+ +AK                          + FVN+GERTN+TGS KF RLI+EE + EAL+VA+ QVE GAQ++D+NMDEGMLD K AMV FLNLIASEPDI+R+P+MIDSSKWE+IEAGLKCIQGKG+VNSIS+KEG E F   A+  K YGAA +VMAFDEDGQAD+  R+ EICKRS D
Sbjct:   26 LDGGMGTMIQSYKLEEADYRGERFADWPSDVKGNNDLLLLSRPDVIQAIEKAYLDAGADILETNTFNATQVSQADYGMQSLAYELNVEGARLARQVADAKTAETPDKPRFVAGVLGPTSRTCSISPDVNNPGYRNVTFDELVENYVEATRGLIEGGADLILIETIFDTLNAKAAIFAVQGVFEELGVELPIMISGTITDASGRTLSGQTTEAFWNSVRHARPISVGLNCALGAKELRPYIEELSTKADTHVSAHPNAGLPNAFGEYDESPAEMAVVVEEFAAAGFLNIVGGCCGTTPAHIEAIAKAVAKYPPRAIPEIPRACRLSGLEPFTIDRSSLFVNVGERTNITGSAKFARLIREENYAEALEVAQQQVEAGAQVIDINMDEGMLDSKAAMVTFLNLIASEPDISRVPIMIDSSKWEVIEAGLKCIQGKGIVNSISMKEGVEAFKHHARLCKRYGAAVVVMAFDEDGQADTQARKEEICKRSYD 513          

BLAST of methionine synthase isoform 3 vs. SwissProt
Match: gi|34395941|sp|P13009.5|METH_ECOLI (RecName: Full=Methionine synthase; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase; AltName: Full=Methionine synthase, vitamin-B12-dependent; Short=MS)

HSP 1 Score: 697.582 bits (1799), Expect = 0.000e+0
Identity = 351/603 (58.21%), Postives = 438/603 (72.64%), Query Frame = 0

Query:  461 DNATEALLEYAESVKG---DGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKE-FVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNF--SLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            D+ TE LLE AE  +G   D     +   WR  +V  R+ ++LVKGITEFI +DTEE R     PIEVIEGPLMDGMNVVGDLFG GKMFLPQVVKSARVMKQAVAYL PFIE +K  + K NGK+++ATVKGDVHDIGKNIVGVVL CNNY+IVD+GVMVP + I++ A E N D+IGLSGLITPSLDEMV+VA+EME+ G  IPLLIGGATTS+ HTAVKIE+NY SG T++V +ASR+V VV  LL +T ++ FVA  + EYE VR+ H +KK +   V  + A +N    DW+A   YTP             S+  L  +IDWTPFF TW LAG+YPRIL DE+VG +A  LF DA +ML K+  EK L  +GV+G++PAN  G DD+ I+ +E RT V    + LRQQT+K     N+ LADF+APK +G  DYIG F VT GL  +     F+  HDDY+ I++KALADRLAEAFAE +H++VR+  WGYAPNE L NEELI+E YQGIRPAPGYPACP+HTEK T+++ L   K  G++LTES AM+P ASVSG+Y++HP SKY+ + +I+ DQV+D A+RKGM   ++++WL P
Sbjct:  625 DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEARQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASKE-QGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNADLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNY-SGPTVYVQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDFAFDWQA---YTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVEAQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVG-DDIEIYRDETRTHVINVSHHLRQQTEKT-GFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMVKALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHTEKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYARRKGMSVTEVERWLAP 1220          

HSP 2 Score: 594.734 bits (1532), Expect = 0.000e+0
Identity = 305/542 (56.27%), Postives = 378/542 (69.74%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIAELAK---------------------------TNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSNDNATEALLEYAESVKGD------------GKKKERDLTWRETDVKGRITHALVKG 503
            LDG MGTMIQ Y+L E DFR   F D    LKGNNDLL L++P++I AIH  YFEAGADI+ETNTF+ TTIA  DY  E    EIN+ +AK+AR  ADE+TA+ P KPR+VAG +GPTN+TASISP +NDP +R I+FD LV A++   +ALVEGG D++L+ETV DTLNAKAA+FA++  F+   +ELPIM+SGTITD SGRTLSGQTTEAF  S  HA   + GLNCALG  +LR Y+Q +++ +   V+AHPNAGLPN FGEYD   + MA+QI+ +   G LNIVGGCCGT+P HI A++   +                           + FVN+GERTNVTGS KF RLIKEEK+ EALDVAR QVE GAQI+D+NMDEGMLD + AMV+FLNLIA EPDIAR+P+MIDSSKW++IE GLKCIQGKG+VNSIS+KEG + FI  AK ++ YGAA +VMAFDE GQAD+  R+IEIC+R+    TE +    E +  D                 +D      D+K  + HAL+ G
Sbjct:   19 LDGGMGTMIQSYRLNEADFRGERFADWPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAEINFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLVAAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDASGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAGLPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLPEIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVENGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQGKGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKILTEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISG 560          

BLAST of methionine synthase isoform 3 vs. SwissProt
Match: gi|17865736|sp|P37586.3|METH_SALTY (RecName: Full=Methionine synthase; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase; AltName: Full=Methionine synthase, vitamin-B12 dependent; Short=MS)

HSP 1 Score: 696.041 bits (1795), Expect = 0.000e+0
Identity = 352/603 (58.37%), Postives = 439/603 (72.80%), Query Frame = 0

Query:  461 DNATEALLEYAESVKG---DGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKE-FVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNF--SLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            D+ TE LL+ AE  +G   D     +   WR  DVK R+ ++LVKGITEFI +DTEE R     PIEVIEGPLMDGMNVVGDLFG GKMFLPQVVKSARVMKQAVAYL PFIE +K  +   NGK+++ATVKGDVHDIGKNIVGVVL CNNY+IVD+GVMVP + I++ A E N D+IGLSGLITPSLDEMV+VA+EME+ G  IPLLIGGATTS+ HTAVKIE+NY SG T++V +ASR+V VV  LL +T ++ FVA  + EYE VR+ HA+KK +   V  + A +N L  DW   E YTP             S+  L  +IDWTPFF TW LAG+YPRIL DE+VG +A  LF DA +ML K+  EK L  +GV+G++PAN  G DD+ I+ +E RT V    + LRQQT+K     N+ LADF+APK +G  DYIG F VT GL  +     F+  HDDY+ I++KA+ADRLAEAFAE +H++VR+  WGYAPNE+L N+ELI+E YQGIRPAPGYPACP+HTEK T+++ L+  K  G++LTES AM+P ASVSG+Y++HP+SKYF + +I+ DQV D A RKGM    +++WL P
Sbjct:  625 DDGTERLLDLAEKYRGSKTDEAANAQQAEWRSWDVKKRLEYSLVKGITEFIEQDTEEARQQAARPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASKE-KGSSNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAREVNADLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNY-SGPTVYVQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHARKKPRTPPVTLEAARDNDLAFDW---ERYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVEAQRLFKDANDMLDKLSAEKLLNPRGVVGLFPANRVG-DDIEIYRDETRTHVLTVSHHLRQQTEKV-GFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMVKAIADRLAEAFAEYLHERVRKVYWGYAPNESLSNDELIRENYQGIRPAPGYPACPEHTEKGTIWQLLDVEKHTGMKLTESFAMWPGASVSGWYFSHPESKYFAVAQIQRDQVTDYAFRKGMSVEDVERWLAP 1220          

HSP 2 Score: 599.742 bits (1545), Expect = 0.000e+0
Identity = 309/542 (57.01%), Postives = 382/542 (70.48%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIAE---------------------LAKTN------FVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSNDNATEALLEYAESVKGD------------GKKKERDLTWRETDVKGRITHALVKG 503
            LDG MGTMIQ Y+L E+DFR   F D    LKGNNDLL L++P++I AIH  YFEAGADI+ETNTF+ TTIA  DY  E    EINY +AK+AR  ADE+TA+ P KPRFVAG +GPTN+TASISP +NDP +R I+FD+LV A++   +ALVEGG D++L+ETV DTLNAKAA+FA+++ F+   ++LPIM+SGTITD SGRTLSGQTTEAF  S  HA   + GLNCALG  +LR Y+Q +++ +   V+AHPNAGLPN FGEYD   + MA+QI+ + + G LNIVGGCCGT+P HI A++                      L   N      FVN+GERTNVTGS KF RLIKEEK+ EALDVAR QVE GAQI+D+NMDEGMLD + AMV+FL+LIA EPDIAR+P+MIDSSKWE+IE GLKCIQGKG+VNSIS+KEG E FI  AK ++ YGAA +VMAFDE GQAD+  R+IEIC+R+    TE +    E +  D                 +D      D+K  + HAL+ G
Sbjct:   19 LDGGMGTMIQSYRLHEEDFRGERFADWPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYRMESLSAEINYAAAKLARACADEWTARTPEKPRFVAGVLGPTNRTASISPDVNDPAFRNITFDQLVAAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKEEFEALGVDLPIMISGTITDASGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAGLPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPEHIAAMSRAVAGLLPRQLPDIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVESGAQIIDINMDEGMLDAEAAMVRFLSLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQGKGIVNSISMKEGVEAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKILTEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISG 560          

BLAST of methionine synthase isoform 3 vs. SwissProt
Match: gi|81726828|sp|Q87L95.1|METH_VIBPA (RecName: Full=Methionine synthase; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase; AltName: Full=Methionine synthase, vitamin-B12 dependent; Short=MS)

HSP 1 Score: 691.034 bits (1782), Expect = 0.000e+0
Identity = 342/601 (56.91%), Postives = 440/601 (73.21%), Query Frame = 0

Query:  461 DNATEALLEYAESVKGDGKKKERD---LTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSK-EFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            D+ATE LL+ A      G  KE D   L WR   V  R+ HALVKGITEFIV DTEE R+   +P+EVIEGPLMDGMNVVGDLFG GKMFLPQVVKSARVMKQAVA+L PFI   K      NGK+L+ATVKGDVHDIGKNIVGVVL CNNY+I+D+GVMVP + I+K AIEENVD+IGLSGLITPSLDEMV VA+EME+    +PLLIGGATTS+ HTAVKIE+NYK+   ++V +ASR+V V   LL +  +  F+  +  +YE+VR  H +KK + + V  + A  N++ IDW A     P   G   F++F ++ L  +IDWTPFF TW L G+YP I   E VGE+A  LF DA E+L ++E+E  L A+G+ G++PA S G DD+ ++T+E RTEV +   +LRQQT+K K   N+ L+D+IAPKE+G QD++G F VT G+G  E   E++   DDY+AI+++A+ADRLAEAFAE +H++VR+E+WGYA +E L N+ELI+EKYQGIRPAPGYPACP+HTEK  L++ LN  + IG+ LT S AMYP ASVSG+Y++HP S+YF + +I+ DQ++  A RKG +  + +KWL P
Sbjct:  626 DDATERLLDIAAEYADKGVGKEEDASALEWRTWPVAKRLEHALVKGITEFIVADTEEARVNAVKPLEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAHLEPFINAEKQSG-SSNGKILLATVKGDVHDIGKNIVGVVLQCNNYEIIDLGVMVPCEKILKVAIEENVDIIGLSGLITPSLDEMVHVAKEMERLNFDLPLLIGGATTSKAHTAVKIEQNYKN-PVVYVNNASRAVGVCSSLLSDERRPAFIEKLDADYERVRDQHNRKKPRTKPVTLEQARANKVAIDWDAYTPPVPAKPGLHIFDDFDVATLRKYIDWTPFFMTWSLVGKYPTIFKHEEVGEEAQRLFHDANELLDRVEREGLLKARGICGLFPAASVG-DDIEVYTDESRTEVAKVLRNLRQQTEKPKGF-NYCLSDYIAPKESGKQDWVGAFAVTGGIGERELADEYKAQGDDYNAIMIQAVADRLAEAFAEYLHERVRKEIWGYAADENLSNDELIREKYQGIRPAPGYPACPEHTEKGPLWELLNVEENIGMSLTTSYAMYPGASVSGWYFSHPDSRYFAIAQIQDDQLESYADRKGWDRIEAEKWLGP 1222          

HSP 2 Score: 609.757 bits (1571), Expect = 0.000e+0
Identity = 291/483 (60.25%), Postives = 364/483 (75.36%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIAELA---------------------------KTNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEIC 456
            +DG MGTMIQ YKL E D+R   F +    LKGNNDLL L++P +IK IH+ Y EAGADI+ETNTF+ TTIA  DY  E    EIN+ +AK+AREVADE+TAK P+KPR+VAG +GPTN+T SISP +NDPGYR +SFDELV A+     AL+ GG D++L+ET+ DTLNAKA  FA++ VF+E  + LP+M+SGTITD SGRTLSGQTTEAF  S  H    S GLNCALG  +LRPY++ +++ S   VSAHPNAGLPN FGEYD +PE MAE +K +   G LN++GGCCGT+P HI+ +A+                             +T F+N+GERTNVTGS +F RLIKEE++DEAL+VAR QVE GAQI+D+NMDEGMLD +  MV+FLNL ASEP+I+++P+M+DSSKWE+IEAGLKCIQGKG+VNSISLKEG+EKF+EQAK ++ YGAA IVMAFDE GQA++  R++EIC
Sbjct:   20 IDGGMGTMIQGYKLEEQDYRGERFANWHCDLKGNNDLLVLSQPQLIKEIHSAYLEAGADILETNTFNATTIAMADYEMESLSEEINFAAAKLAREVADEWTAKTPDKPRYVAGVLGPTNRTCSISPDVNDPGYRNVSFDELVEAYSESTRALIRGGADLILIETIFDTLNAKACAFAVDSVFEELGVALPVMISGTITDASGRTLSGQTTEAFYNSLRHVRPLSFGLNCALGPDELRPYVEELSRISESFVSAHPNAGLPNAFGEYDLSPEDMAEHVKEWASSGFLNLIGGCCGTTPEHIRQMAQAVEGVTPRALPDLPVACRLSGLEPLTIEKETLFINVGERTNVTGSARFKRLIKEEQYDEALEVARQQVENGAQIIDINMDEGMLDAQACMVRFLNLCASEPEISKVPIMVDSSKWEVIEAGLKCIQGKGIVNSISLKEGKEKFVEQAKLIRRYGAAVIVMAFDEVGQAETRTRKLEIC 502          

BLAST of methionine synthase isoform 3 vs. SwissProt
Match: gi|75463102|sp|Q9AJQ8.1|METH_ALIFS (RecName: Full=Methionine synthase; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase; AltName: Full=Methionine synthase, vitamin-B12 dependent; Short=MS)

HSP 1 Score: 690.649 bits (1781), Expect = 0.000e+0
Identity = 346/603 (57.38%), Postives = 446/603 (73.96%), Query Frame = 0

Query:  461 DNATEALL----EYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEV-KPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSK-EFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            D++TE LL    EY E   G  + K   L WR+  V+ R+ H+LVKGITEFIVEDTEE R+    PIEVIEGPLMDGMNVVGDLFG GKMFLPQVVKSARVMKQAVA+L PFI  +K  EV   NGK+L+ATVKGDVHDIGKNIVGVVL CNNY+I+D+GVMV  + I+K A EENVD+IGLSGLITPSLDEMV VA+EME+ G  +PLLIGGATTS+ HTAVKIE+NY S   ++V +ASR+V V   LL N  K  FV  +  +YE+VR  H++K+ + + V  ++A  N++ IDW +     P   G   F+NF +S L  +IDWTPFF TW L G+YP+IL  E VGE+A  LF DA ++L ++EKE  L A+G+  ++PA+S G DD+ ++T+E RT V +  ++LRQQT+K K   N+ L+D+IAPKE+G  D+IGGF VT G+G  E   E++   DDY+AI+++A+ADRLAEAFAE +H+KVR+E+WGY+PNETL N++LI+EKYQGIRPAPGYPACP+HTEK  L++ +N  + IG+ LT S AM+P ASVSG Y++HP S+YF + +I+ DQ +  A RKG    + +KWL P
Sbjct:  627 DDSTERLLDIATEYLERAVGKVEDKSA-LEWRDWPVEKRLEHSLVKGITEFIVEDTEEARINAERPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAHLEPFINASK--EVGATNGKILLATVKGDVHDIGKNIVGVVLQCNNYEIIDLGVMVSCETILKVAKEENVDIIGLSGLITPSLDEMVHVAKEMERQGFDLPLLIGGATTSKAHTAVKIEQNY-SQPVVYVNNASRAVGVCTSLLSNELKPSFVEKLDIDYERVREQHSRKQPRTKPVTLEVARANKVAIDWASYTPPVPLKPGVHIFDNFDVSTLRNYIDWTPFFMTWSLVGKYPKILEHEEVGEEAKRLFKDANDLLDRVEKEGLLKARGMCALFPASSVG-DDIEVYTDESRTTVAKVLHNLRQQTEKPKGF-NYCLSDYIAPKESGKNDWIGGFAVTGGIGERELADEYKANGDDYNAIMIQAVADRLAEAFAEYLHEKVRKEIWGYSPNETLSNDDLIREKYQGIRPAPGYPACPEHTEKGALWELMNVEESIGMSLTSSYAMWPGASVSGMYFSHPDSRYFAIAQIQQDQAESYADRKGWNMLEAEKWLGP 1223          

HSP 2 Score: 598.971 bits (1543), Expect = 0.000e+0
Identity = 289/483 (59.83%), Postives = 357/483 (73.91%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIAELAK---------------------------TNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEIC 456
            +DG MGTMIQ YK  E D+R G F      LKGNNDLL L++P II+ IH  Y EAGADI+ETNTF+ TTIA  DY  E    EIN+ +AK+AREVAD++T K PNKPR+VAG +GPTN+T SISP +NDPG+R +SFDELV A+     AL+ GG D++L+ET+ DTLNAKA  FA+E VF+E  I LP+M+SGTITD SGRTLSGQTTEAF  +  H    S GLNCALG  +LR Y+  +++ S   VSAHPNAGLPN FGEYD +PE MAE +  +   G LN++GGCCGT+P HI+ +A + +                           + F+N+GERTNVTGS +F RLIKEE +DEAL VA++QVE GAQI+D+NMDEGMLD +  MV+FLNL ASEP+I+++PVM+DSSKWE+IEAGLKCIQGKG+VNSISLKEG+EKF+ QAK ++ YGAA IVMAFDE GQAD+ ER+IEIC
Sbjct:   21 IDGGMGTMIQGYKFEEKDYRGGRFNQWHCDLKGNNDLLVLSQPQIIRDIHEAYLEAGADILETNTFNATTIAMADYDMESLSEEINFEAAKLAREVADKWTEKTPNKPRYVAGVLGPTNRTCSISPDVNDPGFRNVSFDELVEAYSESTRALIRGGSDLILIETIFDTLNAKACSFAVESVFEELGITLPVMISGTITDASGRTLSGQTTEAFYNALRHVKPISFGLNCALGPDELREYVSELSRISECYVSAHPNAGLPNAFGEYDLSPEDMAEHVAEWASSGFLNLIGGCCGTTPEHIRQMALVVEGVKPRQLPELPVACRLSGLEPLTIEKDSLFINVGERTNVTGSARFKRLIKEELYDEALSVAQEQVENGAQIIDINMDEGMLDAEACMVRFLNLCASEPEISKVPVMVDSSKWEVIEAGLKCIQGKGIVNSISLKEGKEKFVHQAKLIRRYGAAVIVMAFDEVGQADTRERKIEIC 503          

BLAST of methionine synthase isoform 3 vs. SwissProt
Match: gi|75431838|sp|Q5E814.1|METH_VIBF1 (RecName: Full=Methionine synthase; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase; AltName: Full=Methionine synthase, vitamin-B12 dependent; Short=MS)

HSP 1 Score: 689.493 bits (1778), Expect = 0.000e+0
Identity = 345/603 (57.21%), Postives = 448/603 (74.30%), Query Frame = 0

Query:  461 DNATEALL----EYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEV-KPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSK-EFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            D++TE LL    EY E   G  + K   L WR+  V+ R+ H+LVKGITEFIVEDTEE R+   +PIEVIEGPLMDGMNVVGDLFG GKMFLPQVVKSARVMKQAVA+L PFI  +K  EV   NGK+L+ATVKGDVHDIGKNIVGVVL CNNY+I+D+GVMV  + I+K A EENVD+IGLSGLITPSLDEMV VA+EME+ G  +PLLIGGATTS+ HTAVKIE+NY S   ++V +ASR+V V   LL +  K  FV  +  +YE+VR  H++K+ + + V  ++A  N++ IDW +     P   G   F+NF +S L  +IDWTPFF TW L G+YP+IL  E VGE+A  LF DA ++L ++EKE  L A+G+  ++PA+S G DD+ ++T+E RT+V +  ++LRQQT+K K   N+ L+D+IAPKE+G  D+IGGF VT G+G  E   E++   DDY+AI+++A+ADRLAEAFAE +H+KVR+E+WGY+PNETL N++LI+EKYQGIRPAPGYPACP+HTEK  L++ +N  + IG+ LT S AM+P ASVSG Y++HP S+YF + +I+ DQ +  A RKG    + +KWL P
Sbjct:  627 DDSTERLLDIATEYLERAVGKVEDKSA-LEWRDWPVEKRLEHSLVKGITEFIVEDTEEARINAEKPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAHLEPFINASK--EVGATNGKILLATVKGDVHDIGKNIVGVVLQCNNYEIIDLGVMVSCETILKVAKEENVDIIGLSGLITPSLDEMVHVAKEMERQGFDLPLLIGGATTSKAHTAVKIEQNY-SQPVVYVNNASRAVGVCTSLLSDELKPSFVEKLDIDYERVREQHSRKQPRTKPVTLEVARANKVAIDWASYTPPVPLKPGVHIFDNFDVSTLRNYIDWTPFFMTWSLVGKYPKILDHEEVGEEAKRLFKDANDLLDRVEKEGLLKARGMCALFPASSVG-DDIEVYTDESRTKVAKVLHNLRQQTEKPKGF-NYCLSDYIAPKESGKNDWIGGFAVTGGIGERELADEYKANGDDYNAIMIQAVADRLAEAFAEYLHEKVRKEIWGYSPNETLSNDDLIREKYQGIRPAPGYPACPEHTEKGALWELMNVEESIGMSLTSSYAMWPGASVSGMYFSHPDSRYFAIAQIQQDQAESYADRKGWNMLEAEKWLGP 1223          

HSP 2 Score: 598.586 bits (1542), Expect = 0.000e+0
Identity = 289/483 (59.83%), Postives = 358/483 (74.12%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIAELAK---------------------------TNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEIC 456
            +DG MGTMIQ YK  E+D+R   F      LKGNNDLL L++P II+ IH  Y EAGADI+ETNTF+ TTIA  DY  E    EIN+ +AK+AREVAD++T K PNKPR+VAG +GPTN+T SISP +NDPG+R +SFDELV A+     AL+ GG D++L+ET+ DTLNAKA  FA+E VF+E DI LP+M+SGTITD SGRTLSGQTTEAF  +  H    S GLNCALG  +LR Y+  +++ S   VSAHPNAGLPN FGEYD +PE MAE +  +   G LN++GGCCGT+P HI+ +A + +                           + F+N+GERTNVTGS +F RLIKEE +DEAL VA++QVE GAQI+D+NMDEGMLD +  MV+FLNL ASEP+I+++PVM+DSSKWE+IEAGLKCIQGKG+VNSISLKEG+EKF+ QAK ++ YGAA IVMAFDE GQAD+ ER+IEIC
Sbjct:   21 IDGGMGTMIQGYKFEEEDYRGERFNKWHCDLKGNNDLLVLSQPQIIRDIHEAYLEAGADILETNTFNATTIAMADYDMESLSEEINFEAAKLAREVADKWTEKTPNKPRYVAGVLGPTNRTCSISPDVNDPGFRNVSFDELVEAYSESTRALIRGGSDLILIETIFDTLNAKACSFAVESVFEELDITLPVMISGTITDASGRTLSGQTTEAFYNALRHVKPISFGLNCALGPDELREYVSDLSRISECYVSAHPNAGLPNAFGEYDLSPEDMAEHVAEWASSGFLNLIGGCCGTTPEHIRQMALVVEGVKPRQLPELPVACRLSGLEPLTIEKDSLFINVGERTNVTGSARFKRLIKEELYDEALSVAQEQVENGAQIIDINMDEGMLDAEACMVRFLNLCASEPEISKVPVMVDSSKWEVIEAGLKCIQGKGIVNSISLKEGKEKFVHQAKLIRRYGAAVIVMAFDEVGQADTRERKIEIC 503          

BLAST of methionine synthase isoform 3 vs. SwissProt
Match: gi|81587851|sp|Q8DCJ7.1|METH_VIBVU (RecName: Full=Methionine synthase; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase; AltName: Full=Methionine synthase, vitamin-B12 dependent; Short=MS)

HSP 1 Score: 687.952 bits (1774), Expect = 0.000e+0
Identity = 341/601 (56.74%), Postives = 444/601 (73.88%), Query Frame = 0

Query:  461 DNATEALLEYAESVKGDGKKKERD---LTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSK-EFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            D+ATE LLE AE  + +   K+ D   L WR   V+ R+ HALVKGITEFIVEDTEE RL   +P+EVIEGPLMDGMNVVGDLFG GKMFLPQVVKSARVMKQAVA+L PFI  +K      NGK+L+ATVKGDVHDIGKNIVGVVL CNNY+I+D+GVMVP + I+K A E+ VD+IGLSGLITPSLDEMV VA+EME+ G  +PLLIGGATTS+ HTAVKIE+NY S   ++V +ASR+V V   LL +  +  FV  ++ +YE VR  H +KK + + V  + A  N++ IDW++     P+  G   F++F ++ L  +IDWTPFF TW L G+YP I   E VGE+A  LF DA E L +IE+E  L A+G+ G++PA S G DD+ ++T+E RT V +  ++LRQQT+K K   N+ L+D++APKE+G +D+IG F VT G+   E   +F+   DDY+AI+++A+ADRLAEAFAE +H++VR+E+WGYA +E L NEELI+EKYQGIRPAPGYPACP+HTEK  L++ LN  + IG+ LT S AM+P ASVSG+Y++HP S+YF + +I+ DQV+  A+RKG +  + +KWL P
Sbjct:  626 DDATERLLEIAEEYRENAVGKQEDASALEWRTWSVEKRLEHALVKGITEFIVEDTEEARLNASKPLEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAHLEPFINASKQAG-SSNGKILLATVKGDVHDIGKNIVGVVLQCNNYEIIDLGVMVPCEQILKVAKEQQVDIIGLSGLITPSLDEMVHVAKEMERLGFDLPLLIGGATTSKAHTAVKIEQNY-SHPVVYVNNASRAVGVCTSLLSDELRPAFVERLQADYELVRDQHNRKKPRTKPVTLEAARANKVAIDWQSYTPPAPSQPGVHVFDDFDVATLRQYIDWTPFFLTWSLVGKYPTIFEHEEVGEEAKRLFGDANEWLDRIEQEGLLKARGMCGLFPAASVG-DDIEVYTDESRTHVAKVLHNLRQQTEKPKG-ANYCLSDYVAPKESGKKDWIGAFAVTGGVNERELADQFKAQGDDYNAIMIQAVADRLAEAFAEYLHERVRKEIWGYAADENLSNEELIREKYQGIRPAPGYPACPEHTEKGPLWELLNVEETIGMSLTSSYAMWPGASVSGWYFSHPDSRYFAIAQIQQDQVESYAERKGWDLLEAEKWLGP 1222          

HSP 2 Score: 596.66 bits (1537), Expect = 0.000e+0
Identity = 295/486 (60.70%), Postives = 363/486 (74.69%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIA--------------------------ELAK-TNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRS 459
            +DG MGTMIQ YKL E D+R   F D    LKGNNDLL LT+P +IK IH  Y EAGADI+ETNTF+ TTIA  DY  E    EIN+ +A++ARE ADE+TA+ P KPR+VAG +GPTN+T SISP +NDPGYR +SFDELV A+     AL+ GG D++L+ET+ DTLNAKA  FA++ VF+E    LP+M+SGTITD SGRTLSGQTTEAF  S  H    S GLNCALG  +LRPY++ +++ S   VS HPNAGLPN FGEYD +PE MAE +K +   G LN++GGCCGT+P HI+ +A                           +AK T FVN+GERTNVTGS +F RLIKEE +DEALDVAR+QVE GAQI+D+NMDEGMLD +  MV+FLNL ASEP+I+++P+M+DSSKWE+IEAGLKCIQGKG+VNSISLKEG+EKF+EQAK ++ YGAA IVMAFDE GQAD+ ER++EIC ++
Sbjct:   20 IDGGMGTMIQGYKLQEQDYRGERFADWHSDLKGNNDLLVLTQPQLIKEIHHAYLEAGADILETNTFNATTIAMADYDMESLSEEINFAAARLAREAADEWTAQNPAKPRYVAGVLGPTNRTCSISPDVNDPGYRNVSFDELVEAYSESTRALIRGGSDLILIETIFDTLNAKACAFAVDSVFEELGFALPVMISGTITDASGRTLSGQTTEAFYNSLRHVRPISFGLNCALGPDELRPYVEELSRISETFVSTHPNAGLPNAFGEYDLSPEEMAEHVKEWAQSGFLNLIGGCCGTTPEHIRHMAMAVEGVSPRVLPEIPVACRLSGLEPLTIAKDTLFVNVGERTNVTGSARFKRLIKEELYDEALDVAREQVENGAQIIDINMDEGMLDAEACMVRFLNLCASEPEISKVPIMVDSSKWEVIEAGLKCIQGKGIVNSISLKEGKEKFVEQAKLIRRYGAAVIVMAFDEVGQADTRERKLEICTKA 505          

BLAST of methionine synthase isoform 3 vs. SwissProt
Match: gi|81756509|sp|Q7MHB1.1|METH_VIBVY (RecName: Full=Methionine synthase; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase; AltName: Full=Methionine synthase, vitamin-B12 dependent; Short=MS)

HSP 1 Score: 687.567 bits (1773), Expect = 0.000e+0
Identity = 342/601 (56.91%), Postives = 445/601 (74.04%), Query Frame = 0

Query:  461 DNATEALLEYAESVKGDGKKKERD---LTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSK-EFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            D+ATE LLE AE  + +   K+ D   L WR   V+ R+ HALVKGITEFIVEDTEE RL   +P+EVIEGPLMDGMNVVGDLFG GKMFLPQVVKSARVMKQAVA+L PFI  +K      NGK+L+ATVKGDVHDIGKNIVGVVL CNNY+I+D+GVMVP + I+K A E+ VD+IGLSGLITPSLDEMV VA+EME+ G  +PLLIGGATTS+ HTAVKIE+NY S   ++V +ASR+V V   LL +  +  FV  ++ +YE VR  H +KK + + V  + A  N++ IDW++     P+  G   F++F ++ L  +IDWTPFF TW L G+YP I   E VGE+A  LF DA E L +IE+E  L A+G+ G++PA S G DD+ ++T+E RT+V +  ++LRQQT+K K   N+ L+D++APKE+G +D+IG F VT G+   E   +F+   DDY+AI+++A+ADRLAEAFAE +H++VR+E+WGYA +E L NEELI+EKYQGIRPAPGYPACP+HTEK  L++ LN  + IG+ LT S AM+P ASVSG+Y++HP S+YF + +I+ DQV+  AKRKG +  + +KWL P
Sbjct:  626 DDATERLLEIAEEYRENAVGKQEDASALEWRTWSVEKRLEHALVKGITEFIVEDTEEARLNASKPLEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAHLEPFINASKQVG-SSNGKILLATVKGDVHDIGKNIVGVVLQCNNYEIIDLGVMVPCEQILKVAKEQQVDIIGLSGLITPSLDEMVHVAKEMERLGFDLPLLIGGATTSKAHTAVKIEQNY-SHPVVYVNNASRAVGVCTSLLSDELRPAFVERLQADYELVRDQHNRKKPRTKPVTLEAARANKVAIDWQSYTPPAPSQPGVHVFDDFDVATLRQYIDWTPFFLTWSLVGKYPTIFEHEEVGEEAKRLFEDANEWLDRIEQEGLLKARGMCGLFPAASVG-DDIEVYTDESRTQVAKVLHNLRQQTEKPKG-ANYCLSDYVAPKESGKKDWIGAFAVTGGVNERELADQFKAQGDDYNAIMIQAVADRLAEAFAEYLHERVRKEIWGYAADENLSNEELIREKYQGIRPAPGYPACPEHTEKGPLWELLNVEETIGMSLTSSYAMWPGASVSGWYFSHPDSRYFAIAQIQQDQVESYAKRKGWDLLEAEKWLGP 1222          

HSP 2 Score: 599.356 bits (1544), Expect = 0.000e+0
Identity = 298/486 (61.32%), Postives = 363/486 (74.69%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIA--------------------------ELAK-TNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRS 459
            +DG MGTMIQ YKL E D+R   F D    LKGNNDLL LT+P +IK IH  Y EAGADI+ETNTF+ TTIA  DY  E    EIN+ +AK+ARE ADE+TAK P KPR+VAG +GPTN+T SISP +NDPGYR +SFDELV A+     AL+ GG D++L+ET+ DTLNAKA  FA+E VF+E    LP+M+SGTITD SGRTLSGQTTEAF  S  H    S GLNCALG  +LRPY++ +++ S   VS HPNAGLPN FGEYD +PE MAE +K +   G LN++GGCCGT+P HI+ +A                           +AK T FVN+GERTNVTGS +F RLIKEE +DEALDVAR+QVE GAQI+D+NMDEGMLD +  MV+FLNL ASEP+I+++P+M+DSSKWE+IEAGLKCIQGKG+VNSISLKEG+EKF+EQAK ++ YGAA IVMAFDE GQAD+ ER++EIC ++
Sbjct:   20 IDGGMGTMIQGYKLQEQDYRGERFADWHSDLKGNNDLLVLTQPQLIKEIHHAYLEAGADILETNTFNATTIAMADYDMESLSEEINFAAAKLAREAADEWTAKNPAKPRYVAGVLGPTNRTCSISPDVNDPGYRNVSFDELVEAYSESTRALIRGGSDLILIETIFDTLNAKACAFAVESVFEELGFALPVMISGTITDASGRTLSGQTTEAFYNSLRHVRPISFGLNCALGPDELRPYVEELSRISETFVSTHPNAGLPNAFGEYDLSPEEMAEHVKEWAQSGFLNLIGGCCGTTPEHIRHMAMAVEGESPRVLPEIPVACRLSGLEPLTIAKDTLFVNVGERTNVTGSARFKRLIKEELYDEALDVAREQVENGAQIIDINMDEGMLDAEACMVRFLNLCASEPEISKVPIMVDSSKWEVIEAGLKCIQGKGIVNSISLKEGKEKFVEQAKLIRRYGAAVIVMAFDEVGQADTRERKLEICTKA 505          

BLAST of methionine synthase isoform 3 vs. SwissProt
Match: gi|81623236|sp|Q9KUW9.1|METH_VIBCH (RecName: Full=Methionine synthase; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase; AltName: Full=Methionine synthase, vitamin-B12 dependent; Short=MS)

HSP 1 Score: 684.871 bits (1766), Expect = 0.000e+0
Identity = 338/607 (55.68%), Postives = 439/607 (72.32%), Query Frame = 0

Query:  455 ICKRSNDNATEALLEYAESVKGDGKKKERD---LTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKE-FVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            I  R +D  TE LLE AE+ + +   KE D   L WR   V  R+ HALVKGITEFIV+DTEE R    +P+EVIEGPLMDGMNVVGDLFG GKMFLPQVVKSARVMKQAVAYL PFI   K+G    NGK+L+ATVKGDVHDIGKNIVGVVL CNN++I+D+GVMVP + I+K A E+NVD+IGLSGLITPSLDEMV VA+EME+ G ++PLLIGGATTS+ HTAVKIE+NY +   ++V +ASR+V V   LL +  +  F+  +  +YE+ R  HA+K  + R V  + A  N+  +DW       P   G   F N +L+ L P+IDWTPFF TW L G+YP IL  E VGE+A  LF DA  +L K+E+E  L A G+  ++PA S G DD+ ++++E RT+V    Y+LRQQT+K K   N+ L+D++APKE+G +D+IG F VT G+G       ++   DDY+AI+++A+ADRLAEAFAE +H+KVR+E+WGYA +E L N++LI+E+YQGIRPAPGYPACP+HTEK TL++ LN  + IG+ LT S AM+P ASVSG+Y++HP S+YF + +I+ DQ+   A+RKG    + +KWL P
Sbjct:  621 ILNRRSD-GTERLLEIAEAYRENSVGKEEDASALEWRAWPVAKRLEHALVKGITEFIVQDTEEARQQASKPLEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFINAQKSGSTS-NGKILLATVKGDVHDIGKNIVGVVLQCNNFEIIDLGVMVPCEQILKVAREQNVDIIGLSGLITPSLDEMVHVAKEMERQGFELPLLIGGATTSKAHTAVKIEQNYHA-PVVYVNNASRAVGVCTSLLSDEQRPGFIERLDLDYERTRDQHARKTPKSRPVTLEQARANKAALDWANYTPPAPAKPGVHVFENIALATLRPYIDWTPFFMTWSLMGKYPAILEHEEVGEEAKRLFHDANALLDKVEREGLLKASGMCALFPAASVG-DDIEVYSDESRTQVAHVLYNLRQQTEKPKG-ANYCLSDYVAPKESGKRDWIGAFAVTGGIGERALADAYKAQGDDYNAIMIQAVADRLAEAFAEYLHEKVRKEIWGYASDENLSNDDLIRERYQGIRPAPGYPACPEHTEKATLWQMLNVEETIGMSLTTSYAMWPGASVSGWYFSHPDSRYFAVAQIQPDQLHSYAERKGWRLEEAEKWLAP 1222          

HSP 2 Score: 613.609 bits (1581), Expect = 0.000e+0
Identity = 297/486 (61.11%), Postives = 367/486 (75.51%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIAELA---------------------------KTNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRS 459
            +DG MGTMIQ YKL E+D+R   F D    LKGNNDLL LT+P IIK IH+ Y EAGADI+ETNTF+ TTIA  DY  +    EIN+ +AK+AREVADE+TAK+P++PR+VAG +GPTN+T SISP +NDPG+R ++FD LV A+     AL++GG D++L+ET+ DTLNAKA  FA++ VF+E  I LP+M+SGTITD SGRTLSGQTTEAF  +  H    S GLNCALG  +LR Y++ +++ S   VSAHPNAGLPN FGEYD + E MAE I  +   G LN+VGGCCGT+P HI AIA+                             +T FVN+GERTNVTGS +F RLIKEE++DEALDVAR+QVE GAQI+D+NMDEGMLD +  MV+FLNL ASEP+I+++PVM+DSSKWE+IEAGLKCIQGKG+VNSISLKEG+EKFI QAK V+ YGAA IVMAFDE GQAD+ ER++EIC+R+
Sbjct:   20 IDGGMGTMIQSYKLQEEDYRGARFVDWHCDLKGNNDLLVLTQPQIIKEIHSAYLEAGADILETNTFNSTTIAMADYDMQSLSAEINFAAAKLAREVADEWTAKDPSRPRYVAGVLGPTNRTCSISPDVNDPGFRNVTFDGLVEAYSESTRALIKGGSDLILIETIFDTLNAKACAFAVDSVFEELGISLPVMISGTITDASGRTLSGQTTEAFYNALRHVRPISFGLNCALGPDELRQYVEELSRISECYVSAHPNAGLPNAFGEYDLSAEEMAEHIAEWAQAGFLNLVGGCCGTTPEHIAAIAKAVEGVKPRALPDLKVECRLSGLEPLNIGPETLFVNVGERTNVTGSARFKRLIKEEQYDEALDVAREQVENGAQIIDINMDEGMLDAEACMVRFLNLCASEPEISKVPVMVDSSKWEVIEAGLKCIQGKGIVNSISLKEGKEKFIAQAKLVRRYGAAVIVMAFDEVGQADTRERKLEICRRA 505          

BLAST of methionine synthase isoform 3 vs. SwissProt
Match: gi|74854141|sp|Q54P92.1|METH_DICDI (RecName: Full=Methionine synthase; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase; AltName: Full=Vitamin-B12 dependent methionine synthase; Short=MS)

HSP 1 Score: 629.402 bits (1622), Expect = 0.000e+0
Identity = 318/623 (51.04%), Postives = 426/623 (68.38%), Query Frame = 0

Query:  455 ICKRSNDNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKN-----------GEVKPN--GKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKE-FVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYP-----RILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNED--RTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLN 1056
            I  R+ND ATE LLEYA++     K       WR   V  RI HALVKGIT +I+EDTEE R   P  + VIEGPLM GMNVVGDLFG+GKMFLPQV+KSARVMK+AVA+L PF+EE K             E +P+  G V++ATVKGDVHDIGKNIVGVVLGCNNY ++D+GVM P + I++  I    DV+GLSGLITPSLDEM+ VA E+E+   KIPL+IGGATTS++HTAVKI  +Y S  T+HVLDASRSVTVV+ LL   +KE F  D+  +Y ++R  H      ++  + + A ++ +K++WK  +   P F+G + F  +SL +L+  IDW PFF TWQL G+YP     RI  DE VG +A  LF DAQ ML +I  +K L A+GVIG +PANS  +D +    N+D  R++   T + LRQQ +K  + P  ++ D+IAP  +G++DYIG F V++G G+E+ V++++K +DDYS+I+ KALADRLAEA AE +H+ VR E W Y  ++ L NE+L K KY+GIRPAPGYPA PDHTE  T++  +N  +   +ELT+ MAM P A+V G Y++H  +KYF +GKI  +Q++  A RK + + + ++WL+
Sbjct:  628 ILNRTND-ATEKLLEYAQANNKSEKANVEVEEWRNKPVSERIAHALVKGITTYIIEDTEEARNTLPSSLSVIEGPLMGGMNVVGDLFGAGKMFLPQVIKSARVMKKAVAHLIPFMEEEKRLKRLEKGNDEAAEDEPDNAGVVVLATVKGDVHDIGKNIVGVVLGCNNYKVIDIGVMTPCEKIVEAIIANKADVVGLSGLITPSLDEMIYVASELERLKFKIPLMIGGATTSQIHTAVKISPHY-SQPTVHVLDASRSVTVVQSLLDPNNKEVFAEDVSQQYAELREKHYASLKDRKYTSLEKARQHCVKVNWKTIQPVKPTFLGTQVFKEYSLEKLVTKIDWNPFFVTWQLRGKYPNRGYPRIFNDETVGAEAKKLFDDAQTMLKEIVDKKLLNARGVIGFYPANSIDEDIIIYDHNDDETRSKPIATLFGLRQQNEKETDEPYIAIGDYIAPVSSGVKDYIGLFAVSSGFGLEDMVEKYKKENDDYSSIMAKALADRLAEALAEAVHEDVRREHWAYEKDQALSNEDLFKIKYKGIRPAPGYPAQPDHTEMKTIWSLMNVNENTSIELTDHMAMLPGAAVCGVYFSHEHAKYFSVGKITKEQIESYASRKQITKEEAERWLS 1248          

HSP 2 Score: 578.941 bits (1491), Expect = 0.000e+0
Identity = 287/504 (56.94%), Postives = 362/504 (71.83%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKA-------IAELA--------------------KTNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSNDNATEALLEYAESVKGD 477
            LDGAMGT IQ++KL ++D+R   F+D    L GNNDLLSLT+P II+ IH KY EAGAD +ETNTF+G   +Q DY  E  V  IN  SA++A+   +E+T K+P++PRFV G++GPTNKTASISP +  P  R + FDELV+ +  QVEALVEGG+D++LVETV D+LN KAALFAIE+ F+     LP+ +SGTI D+SGRTLSGQT EAF  S   A +   GLNCALGA+++RP+LQ ++K S   VS +PNAGLPN FG YDETPEMMAEQIK F + GLLNIVGGCCGTSP HI+A       IA  A                    + NFVN+GER NV+GSR+F  LIK  K++EAL VAR QVE GAQI+D+NMDEGM+D   A+ KFL  I SEP+I+++P+M+DSS ++++EAGLKC+QGK +VNSISLK GEE FI+QAK VK YGA+ +VMAFDE+GQA S E ++ IC RS    TE +  Y + +  D
Sbjct:   27 LDGAMGTEIQKFKLKDNDYRGEEFKDFPHELGGNNDLLSLTQPHIIREIHCKYLEAGADFIETNTFNGNIFSQADYKMEHLVKRINIESARLAKSACEEYTKKDPSRPRFVCGAVGPTNKTASISPSVERPEARNVLFDELVSGYLEQVEALVEGGIDVILVETVFDSLNCKAALFAIEEFFKTYSPRLPVFVSGTIVDKSGRTLSGQTGEAFYTSVASANLMVFGLNCALGAQEMRPFLQNISKCSECYVSCYPNAGLPNTFGGYDETPEMMAEQIKEFAESGLLNIVGGCCGTSPDHIRAFCNAIEGIAPRAIPTLVPNTTLSGLEPLVFTKELNFVNVGERCNVSGSRRFANLIKANKYEEALSVARQQVEAGAQIIDINMDEGMIDAVAAIQKFLFFIGSEPEISKVPIMLDSSNFDVVEAGLKCVQGKCIVNSISLKVGEELFIKQAKIVKQYGASVVVMAFDENGQATSKEEKVRICYRSYKILTEQVGFYPQDIIFD 530          

BLAST of methionine synthase isoform 3 vs. nr
Match: gi|837789131|ref|XP_012785394.1| (PREDICTED: methionine synthase isoform X3 [Ochotona princeps])

HSP 1 Score: 1144.41 bits (2959), Expect = 0.000e+0
Identity = 589/1112 (52.97%), Postives = 767/1112 (68.97%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIAELAK-----------------------------TNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSNDNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRL---LYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAK------NGEVKP----NGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLG-NTSKEFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEV-YTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRY-----PRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVD----ETFYSLRQQTQK--AKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            LDG MGTMIQ+YKL E+ FR   F+D    LKGNND+LS+T+PDI+  IH  Y  AGADI+ETNTFS T+IAQ DYG E   Y +N  SA++AR+ A+E T +   K RFVAG++GPTNKT S+SP +  P YR I+FDELV A++ Q   L++GGVD+LL+ET+ DT NAKAA+FA++Q+FQEK    PI +SGTI D+SGRTLSGQT EAF+IS  HA    IGLNCALGA ++RP+++ + K +   V  +PNAGLPN FG+YDETP +MA  +K F   GL+NIVGGCCGT+PAHI+ IAE  K                             TNFVNIGER NV GS+KF +LI    ++EAL VA++QV+ GAQ+LDVNMD+GMLDG  AM +F NLIASEPDIA++P+ IDSS + +IEAGLKC QGK +VNSISLKEGE+ F+E+A+KVK +GAA +VMAFDE+GQ                               DGKK  +   WR   V+ R+ +ALVKGI ++I+EDTEE RL    YP P+ +IEGPLM+GM VVGDLFG+GKMFLPQV+KSARVMK+AV +L PF+E+ +      NG ++      G +++ATVKGDVHDIGKNIVGVVLGCNN+ ++D+GVM P   I+K A++   D+IGLSGLITPSLDEM+ VA+EME+  +KIPLLIGGATTSR HTAVKI   Y S   +HVLDAS+SV V  +LL  N   ++  +I  EYE +R +H +   ++R +    A +N  +IDW +E     P FIG + F ++ L +L+ +IDW PFF  WQL G+Y     P+I  D+ VGE+A  ++ DAQ ML  +  EK L AKG++G WPA S  +DD+ ++  ED    D     TFY LRQQ +K  A   P   LADFIAP  TG++DY+G F V A  G+E+  K +++  DDYS+I++KAL DRLAEAFAE +H++VR ELW Y  +E LD  +L + +Y+GIRPAPGYP+ PDHTEKLT+++  N  +  G+ LTES+AM PA++VSG Y+++ +SKYF +GKI  DQ++D A RK M  A+++KWL P
Sbjct:    3 LDGGMGTMIQRYKLSEEHFRDEEFKDHARSLKGNNDILSITQPDIVYQIHKDYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNRCSARLARKAAEEVTLQTGIK-RFVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYQEQARGLLDGGVDILLIETIFDTANAKAAVFALQQLFQEKYTPRPIFISGTIVDKSGRTLSGQTGEAFVISVSHADPLCIGLNCALGAAEMRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPTIMATHLKDFAMNGLVNIVGGCCGTTPAHIREIAETVKNCKPRVPPASLFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSKKFAKLIMAGNYEEALSVAKEQVQMGAQVLDVNMDDGMLDGPSAMSRFCNLIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKVKKFGAAVVVMAFDEEGQTHG---------------------------KDGKKNVQSDEWRNNPVEERLEYALVKGIEKYIIEDTEEARLNQEKYPRPLNIIEGPLMNGMKVVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREANRIQNGTLEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRY-SAPVVHVLDASKSVVVCSQLLDENLKDDYFEEIIEEYEDIRQDHYESLKERRYLPLIQARKNAFQIDWLSEPYPVKPTFIGTQVFEDYDLQKLVNYIDWKPFFDVWQLRGKYPNRGFPKIFDDKTVGEEARKVYDDAQNMLNTVISEKKLQAKGIVGFWPAQSV-QDDIHLYA-EDAVPQDAEPIATFYGLRQQAEKDSASTDPYHCLADFIAPLHTGVRDYLGLFAV-ACFGVEKLSKAYEENGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYEGIRPAPGYPSQPDHTEKLTMWRLANIEESTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQIEDYALRKKMSVAEVEKWLGP 1082          

BLAST of methionine synthase isoform 3 vs. nr
Match: gi|194389568|dbj|BAG61745.1| (unnamed protein product [Homo sapiens])

HSP 1 Score: 1125.92 bits (2911), Expect = 0.000e+0
Identity = 574/1110 (51.71%), Postives = 760/1110 (68.47%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIAELAK-----------------------------TNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSNDNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRL---LYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAK------NGEVKP----NGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLG-NTSKEFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAE-EVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRY-----PRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDE---TFYSLRQQTQK--AKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLN 1056
            LDG MGTMIQ+ KL E+ FR   F+D   PLKGNND+LS+T+PD+I  IH +Y  AGADI+ETNTFS T+IAQ DYG E   Y +N  SA +AR+ A+E T +   K RFVAG++GPTNKT S+SP +  P YR I+FDELV A++ Q + L++GGVD+LL+ET+ DT NAKAALFA++ +F+EK    PI +SGTI D+SGRTLSGQT E F+IS  H     IGLNCALGA ++RP+++++ K +   V  +PNAGLPN FG+YDETP MMA+ +K F  +GL+NIVGGCCG++P HI+ IAE  K                             TNFVNIGER NV GSRKF +LI    ++EAL VA+ QVE GAQ+LDVNMD+GMLDG  AM +F NLIASEPDIA++P+ IDSS + +IEAGLKC QGK +VNSISLKEGE+ F+E+A+K+K YGAA +VMAFDE+GQ                                GKK  +   WR   V+ R+ +ALVKGI + I+EDTEE RL    YP P+ +IEGPLM+GM +VGDLFG+GKMFLPQV+KSARVMK+AV +L PF+E+ +      NG V+      G +++ATVKGDVHDIGKNIVGVVLGCNN+ ++D+GVM P   I+K A++   D+IGLSGLITPSLDEM+ VA+EME+  ++IPLLIGGATTS+ HTAVKI   Y S   IHVLDAS+SV V  +LL  N   E+  +I  EYE +R +H +   ++R +    A ++  ++DW +E     P FIG + F ++ L +L+ +IDW PFF  WQL G+Y     P+I  D+ VG +A  ++ DA  ML  +  +K L A+GV+G WPA S  +DD+ ++      +  E   TFY LRQQ +K  A   P + L+DFIAP  +G++DY+G F V A  G+EE  K ++   DDYS+I++KAL DRLAEAFAE +H++VR ELW Y  +E LD  +L + +Y+GIRPAPGYP+ PDHTEKLT+++  +  +  G+ LTES+AM PA++VSG Y+++ +SKYF +GKI  DQV+D A RK +  A+++K ++
Sbjct:   33 LDGGMGTMIQREKLNEEHFRGQEFKDHARPLKGNNDILSITQPDVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEEVTLQTGIK-RFVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYQEQAKGLLDGGVDILLIETIFDTANAKAALFALQNLFEEKYAPRPIFISGTIVDKSGRTLSGQTGEGFVISVSHGEPLCIGLNCALGAAEMRPFIEIIGKCTTAYVLCYPNAGLPNTFGDYDETPSMMAKHLKDFAMDGLVNIVGGCCGSTPDHIREIAEAVKNCKPRVPPATAFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALCVAKVQVEMGAQVLDVNMDDGMLDGPSAMTRFCNLIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKYGAAMVVMAFDEEGQTQG---------------------------TGGKKVIQTDEWRNGPVEERLEYALVKGIEKHIIEDTEEARLNQKKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETRVLNGTVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIRIPLLIGGATTSKTHTAVKIAPRY-SAPVIHVLDASKSVVVCSQLLDENLKDEYFEEIMEEYEDIRQDHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRARGVVGFWPAQSI-QDDIHLYAEAAVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYLGLFAV-ACFGVEELSKAYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYKGIRPAPGYPSQPDHTEKLTMWRLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQVEDYALRKNISVAEVEKCMS 1111          

BLAST of methionine synthase isoform 3 vs. nr
Match: gi|1270354678|gb|PHR49565.1| (methionine synthase [Fluviicola sp.])

HSP 1 Score: 877.085 bits (2265), Expect = 0.000e+0
Identity = 414/604 (68.54%), Postives = 500/604 (82.78%), Query Frame = 0

Query:  454 EICKRSNDNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKEFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            ++     D+ATE LLEYAE+ KG+GKK+E DL+WR+  V  R+ HALVKGI EFI EDTEECR  +  PIEVIEGPLMDGMN+VGDLFGSGKMFLPQVVKSARVMK+AVAYL PFIE  K    KPNG++LMATVKGDVHDIGKNIVGVVLGCNNYD++DMGVMVP + II KAIEENVDVIGLSGLITPSLDEMV++A+EM++A LKIPLLIGGATTSRVHTAVKIEE+Y++ QT+HVLDASRSVTVVE LLG    EFV +IK +Y K+RV+HAK +  K  ++   A +N+++IDWKAE+VY PN +G + F N+ L+EL+ +IDWTPFFQTW+LAGRYP ILTDE+VGE AT LF DA++ML KI  EKWL A+ VIG++PANS   DD+ I+T+E RTEV    +++RQQ +K+    N +L+DF+APKETGL+DYIGGFVVT GLGIEEH+  F+  HDDY++I+LKALADRLAEAFAE MH++VR+E WGYA +E  +N+ LIKEKYQGIRPAPGYPACPDHTEK  LF+ LNAT+  GV LTESMAM PAA+VSG+Y+AHP+SKYFG+GKI  DQV+ +A+RKGM   +M+KWL P
Sbjct:  286 DVLLNRRDDATERLLEYAENFKGEGKKREVDLSWRDQPVNDRLAHALVKGIVEFIEEDTEECRHQFERPIEVIEGPLMDGMNIVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPFIEADKREGDKPNGRILMATVKGDVHDIGKNIVGVVLGCNNYDVIDMGVMVPAEKIIAKAIEENVDVIGLSGLITPSLDEMVNMAKEMQRADLKIPLLIGGATTSRVHTAVKIEEHYQNNQTVHVLDASRSVTVVESLLGQKRDEFVKNIKADYNKLRVHHAKSRASKSYLSLADARKNKIQIDWKAEDVYAPNELGLREFVNYDLAELVDYIDWTPFFQTWELAGRYPNILTDEVVGEAATKLFEDAKKMLQKIIDEKWLEARAVIGLFPANSVNDDDIEIYTDESRTEVLSVQHAMRQQLKKSSKADNIALSDFVAPKETGLKDYIGGFVVTTGLGIEEHIARFEADHDDYNSIMLKALADRLAEAFAERMHERVRKEFWGYAADEKFENDALIKEKYQGIRPAPGYPACPDHTEKPDLFRILNATETTGVSLTESMAMTPAAAVSGWYFAHPESKYFGVGKIAKDQVESLAERKGMSFDEMEKWLGP 889          

HSP 2 Score: 278.1 bits (710), Expect = 1.657e-75
Identity = 132/154 (85.71%), Postives = 147/154 (95.45%), Query Frame = 0

Query:  308 KTNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSND 461
            ++NF+N+GERTNVTGS KFLRLIKE+ ++EAL+VA DQV+GGAQI+DVNMDEGM+DGKEAMVKFLNLIASEPDIAR+PVM+DSSKWEIIEAGLKCIQGKGVVNSISLKEGEE+FIE AKKVK YGAA IVMAFDEDGQADS+ERRIEICKRS D
Sbjct:   21 ESNFINVGERTNVTGSAKFLRLIKEDNYEEALEVALDQVQGGAQIIDVNMDEGMIDGKEAMVKFLNLIASEPDIARVPVMVDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEQFIEHAKKVKRYGAAVIVMAFDEDGQADSFERRIEICKRSYD 174          

BLAST of methionine synthase isoform 3 vs. nr
Match: gi|1316693333|ref|WP_101335588.1| (methionine synthase [Brumimicrobium sp. LHR20] >gi|1315119969|gb|PKR79762.1| methionine synthase [Brumimicrobium sp. LHR20])

HSP 1 Score: 827.395 bits (2136), Expect = 0.000e+0
Identity = 388/595 (65.21%), Postives = 482/595 (81.01%), Query Frame = 0

Query:  461 DNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKEFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWL 1055
            ++ATE LL++AE+  G+ K+K  DL WR   VK R+ HALVKGITE+I EDTEECR  Y  PIEVIEGPLMDGMN+VGDLFGSGKMFLPQVVKSARVMK+AVAYL P+IE+ K    KPNGK++MATVKGDVHDIGKNIVGVVLGCNNYD++D+GVMVP + II+ AIE N D IGLSGLITPSLDEMV+VA+EME+A + IPLLIGGATTSRVHTAVKIE+ Y   QT+H+LDASRSVT VE LLGN   EFV++IK EY+K+R +H K K +K  +  + A  N+  I+WK  ++  PN +G   F ++SL E++ + DWTPFFQ W+LAGR+P+IL DE+VGE AT L+ +AQE+L  I +EKWLTAKGV G++PANS   DD+ I+T+E RTEV    +S+R Q +K+K   N +LADFIAPKE+G++DYIGGFVVT+G+GI+EH+K F+  HDDY+AI+L+ALADRLAE+FAEL+H +VR+E WGY+P+E LDNE LI+EKY+GIRPAPGYPACPDHTEK  LFK LNAT+   VELTE MAMYP A+V+G+Y+ HP++KYFG+GKI  DQV+ +AKRKG    +M+KWL
Sbjct:  295 EDATERLLDFAENFTGEKKEKVVDLAWRNQPVKDRLAHALVKGITEYIEEDTEECRHNYKRPIEVIEGPLMDGMNIVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPYIEDDKQEGDKPNGKIVMATVKGDVHDIGKNIVGVVLGCNNYDVIDLGVMVPAEKIIQTAIENNADAIGLSGLITPSLDEMVTVAKEMERADIDIPLLIGGATTSRVHTAVKIEQFYTKNQTVHILDASRSVTAVESLLGNKRNEFVSNIKEEYKKLREHHLKSKSRKTYIPLEEARANKFPIEWKQSDIKVPNQLGVFNFEDYSLEEILEYTDWTPFFQAWELAGRFPKILEDEVVGESATKLYEEAQELLKTIIEEKWLTAKGVYGLFPANSINDDDIEIYTDESRTEVLHVQHSMRSQLKKSKKAHNLALADFIAPKESGIKDYIGGFVVTSGIGIDEHIKRFKDDHDDYNAIMLQALADRLAESFAELLHARVRKEFWGYSPDEDLDNEALIREKYKGIRPAPGYPACPDHTEKPGLFKILNATEHTTVELTEHMAMYPTAAVAGWYFGHPEAKYFGVGKIAKDQVESLAKRKGKSVEEMEKWL 889          

HSP 2 Score: 270.011 bits (689), Expect = 1.232e-72
Identity = 126/155 (81.29%), Postives = 147/155 (94.84%), Query Frame = 0

Query:  307 AKTNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSND 461
            +++NF+N+GERTNVTGS+KFLRLIKE+K++EAL+VA DQVEGGAQI+DVNMDEGM+DGKEAMVKFLNLIASEPDIAR+P+M+DSSKWEIIEAGLKC+QGKGVVNSISLK+GEE F E+AK ++  GAA IVMAFDEDGQAD+YERRIEICKRS D
Sbjct:   22 SESNFINVGERTNVTGSKKFLRLIKEDKYEEALEVALDQVEGGAQIIDVNMDEGMIDGKEAMVKFLNLIASEPDIARVPIMVDSSKWEIIEAGLKCVQGKGVVNSISLKDGEELFKERAKTIRRLGAAVIVMAFDEDGQADNYERRIEICKRSYD 176          

BLAST of methionine synthase isoform 3 vs. nr
Match: gi|1222197964|ref|WP_090252966.1| (methionine synthase [Lishizhenia tianjinensis] >gi|1097373168|emb|SFT90073.1| methionine synthase (B12-dependent) [Lishizhenia tianjinensis])

HSP 1 Score: 825.469 bits (2131), Expect = 0.000e+0
Identity = 410/617 (66.45%), Postives = 500/617 (81.04%), Query Frame = 0

Query:  441 DEDGQADSYERRIEICKRSNDNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKEFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            D D +  +Y   + + +R +  ATE LL++AE+VKGDGKKKE+DL+WRET V+ R+ HALVKGI EFI EDTEECR  +  PI+VIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMK+AVAYL P+IE  K+G     GKVLMATVKGDVHDIGKNIVGVVLGCNNYD++D+GVMV  + II+ AI+ENVDVIGLSGLITPSLDEMV+VA+EM++A LKIPL+IGGATTSRVHTAVKIEENY++ QT+HVLDASRSVTVVE LLG    ++VA++K EY+K+R +HAK + +K  +    A  N++ I WKAE+VYTP  +G   F ++SL EL+ FIDWTPFFQTW+LAG +PRIL DEIVGE AT L+ DA+ ML +I  EKWL AK V G++PAN+ G DD+ I+ +E R+EV    ++LRQQ +K+ N  N +L+DF+APKE+GL+DYIG FVVT G+GI+E VK F+  HDDY++I+LKALADRLAEAFAE +H+ VR E WGYA NE+LDNE LIKEKYQGIRPAPGYPACPDHTEK TLF+ LNAT+  GV LTES+AM P ASVSG+Y+AHPQ+KYFG+GKI  +QV+ IA RKG    +M+KWL P
Sbjct:  277 DIDKELLTYVEDVLLNRRPD--ATERLLDFAETVKGDGKKKEKDLSWRETTVEKRLEHALVKGIVEFIEEDTEECRQKFDRPIQVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPYIEAEKDGVQSAAGKVLMATVKGDVHDIGKNIVGVVLGCNNYDVIDLGVMVSAEKIIETAIKENVDVIGLSGLITPSLDEMVTVAKEMQRADLKIPLMIGGATTSRVHTAVKIEENYQNDQTVHVLDASRSVTVVESLLGQRKADYVANVKAEYKKLRDHHAKNRGRKTYLPLTQAQANKMNISWKAEDVYTPKQLGVHHFEDYSLEELVDFIDWTPFFQTWELAGPFPRILEDEIVGEAATKLYEDARAMLKQIVDEKWLQAKAVFGLFPANTVG-DDIEIYKDETRSEVLAVQHTLRQQLKKSANADNLALSDFVAPKESGLEDYIGAFVVTTGIGIDERVKAFEADHDDYNSIMLKALADRLAEAFAEKLHQDVRREHWGYAANESLDNEALIKEKYQGIRPAPGYPACPDHTEKETLFELLNATELTGVSLTESLAMLPTASVSGWYFAHPQAKYFGVGKITEEQVQSIADRKGKSFEEMEKWLRP 890          

HSP 2 Score: 268.085 bits (684), Expect = 5.263e-72
Identity = 127/152 (83.55%), Postives = 140/152 (92.11%), Query Frame = 0

Query:  310 NFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSND 461
            NF+NIGERTNVTGSRKFLRLI+EE ++EAL VA +QVEGGAQI+DVNMDEGMLDG EAM KFLNLIASEPDIAR+P+M+DSSKWEII AGLKCIQGKGVVNSISLKEGEE FI QAK +K +GAA IVMAFDE+GQADSYERR+EICKRS D
Sbjct:   25 NFINIGERTNVTGSRKFLRLIQEENYEEALSVALEQVEGGAQIIDVNMDEGMLDGVEAMTKFLNLIASEPDIARVPIMVDSSKWEIIVAGLKCIQGKGVVNSISLKEGEENFIYQAKTIKRFGAAVIVMAFDENGQADSYERRVEICKRSYD 176          

BLAST of methionine synthase isoform 3 vs. nr
Match: gi|1200339042|gb|OUU59318.1| (methionine synthase [Verrucomicrobia bacterium TMED60])

HSP 1 Score: 822.387 bits (2123), Expect = 0.000e+0
Identity = 386/594 (64.98%), Postives = 485/594 (81.65%), Query Frame = 0

Query:  461 DNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKEFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKW 1054
            +++TE LLEYAE+VKG GKKK  DL+WR+ DV+ R++H+LVKGI EFI ED EECR  Y  PI+VIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMK+AVAYL PFIEE K G+V+ +GK+LMATVKGDVHDIGKNIVGVVLGCNNY+I+D+GVMVP + II  A EENVD+IGLSGLITPSLDEMV VA+E+EKA L IPLLIGGATTS+VHTAVKI+E+Y  GQT+HVLDASRSVTV E LLG     F+ ++ T+YE++R+ H K    K+L++   A  N L++++ +E +  P  +G    +N S++ELIPFIDWTPFFQTW+L GRYP +L DE+VG++A  LF+DAQEML KI+KE W+ AKGV+G++PANS G DD+ I+++  R++   T  +LRQQT+KAK  PN +LADFIAPK T + D+IG F VT+GLGIE+++ +F+K HDDY++ILLKALADRLAEAFAE +H++VR  +W YA NE   NEELIKEKY GIRPAPGYPACPDHTEK+ LF+ LN ++ IG+ LT+S+AM PA+SVSG+Y+AHP SKYFGLGK+  +Q++DI++RKG++    KKW
Sbjct:  291 EDSTERLLEYAETVKGSGKKKTIDLSWRKQDVEARLSHSLVKGIIEFIDEDVEECRTKYERPIQVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPFIEEEKGGKVESSGKILMATVKGDVHDIGKNIVGVVLGCNNYEIIDLGVMVPAEKIIAVAQEENVDIIGLSGLITPSLDEMVHVAQELEKAELNIPLLIGGATTSKVHTAVKIDEHYNRGQTVHVLDASRSVTVAESLLGEKKDTFIDELITDYERLRLQHEKHLKAKKLISVADARANALRLNFNSETIQEPKNLGVTIVDNVSIAELIPFIDWTPFFQTWELHGRYPNVLEDEVVGKEAVQLFSDAQEMLDKIQKENWMKAKGVLGLFPANSVG-DDIEIYSDTKRSKTLYTQRTLRQQTKKAKGQPNLALADFIAPKSTEVIDFIGAFAVTSGLGIEKYITQFEKDHDDYNSILLKALADRLAEAFAEYLHQEVRTNIWAYAENENFSNEELIKEKYAGIRPAPGYPACPDHTEKIGLFELLNVSESIGISLTDSLAMMPASSVSGWYFAHPDSKYFGLGKVNEEQIRDISQRKGLDFEVNKKW 883          

HSP 2 Score: 254.988 bits (650), Expect = 1.570e-67
Identity = 120/153 (78.43%), Postives = 136/153 (88.89%), Query Frame = 0

Query:  309 TNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSND 461
            +NFVNIGERTNVTGSR FLRLI+   ++ A+ VA +QV GGAQI+D+NMDEGM+DGKEAMV FLNLIA+EPDIAR+P+MIDSSKWEIIEAGLKCIQGKG+VNSISLKEGE +FI QA  +K +GAA IVMAFDE GQADSYERRIEICKRS D
Sbjct:   20 SNFVNIGERTNVTGSRAFLRLIQSGDYESAVAVALEQVNGGAQIIDINMDEGMIDGKEAMVTFLNLIAAEPDIARVPIMIDSSKWEIIEAGLKCIQGKGIVNSISLKEGETQFIAQANTIKNFGAAVIVMAFDEQGQADSYERRIEICKRSYD 172          

BLAST of methionine synthase isoform 3 vs. nr
Match: gi|1200248756|gb|OUT72048.1| (methionine synthase [Crocinitomicaceae bacterium TMED16])

HSP 1 Score: 822.002 bits (2122), Expect = 0.000e+0
Identity = 386/594 (64.98%), Postives = 484/594 (81.48%), Query Frame = 0

Query:  461 DNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKEFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKW 1054
            +++TE LLEYAE+VKG GKKK  DL+WR+ DV+ R++H+LVKGI EFI ED EECR  Y  PI+VIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMK+AVAYL PFIEE K G+V+ +GK+LMATVKGDVHDIGKNIVGVVLGCNNY+I+D+GVMVP + II  A EENVD+IGLSGLITPSLDEMV VA+E+EKA L IPLLIGGATTS+VHTAVKI+E+Y  GQT+HVLDASRSVTV E LLG     F+ ++ T+YE++R+ H K    K+L++   A  N L++++ +E +  P  +G    +N S++ELIPFIDWTPFFQTW+L GRYP +L DE+VG++A  LF+DAQEML KI+KE W+ AKGV+G++PANS G DD+ I+++  R++   T  +LRQQT+KAK  PN +LADFIAPK T + D+IG F VT+GLGIE+++ +F+K HDDY++ILLKALADRLAEAFAE +H +VR  +W YA NE   NEELIKEKY GIRPAPGYPACPDHTEK+ LF+ LN ++ IG+ LT+S+AM PA+SVSG+Y+AHP SKYFGLGK+  +Q++DI++RKG++    KKW
Sbjct:  291 EDSTERLLEYAETVKGSGKKKTIDLSWRKQDVEARLSHSLVKGIVEFIDEDVEECRTKYERPIQVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPFIEEEKGGKVESSGKILMATVKGDVHDIGKNIVGVVLGCNNYEIIDLGVMVPAEKIIAVAQEENVDIIGLSGLITPSLDEMVHVAQELEKAELNIPLLIGGATTSKVHTAVKIDEHYNRGQTVHVLDASRSVTVAESLLGEKKDAFIDELITDYERLRLQHEKHLKAKKLISVADARANALRLNFNSETIQEPKNLGVTIVDNVSIAELIPFIDWTPFFQTWELHGRYPNVLEDEVVGKEAVQLFSDAQEMLDKIQKENWMKAKGVLGLFPANSVG-DDIEIYSDTKRSKTLYTQRTLRQQTKKAKGQPNLALADFIAPKSTEVIDFIGAFAVTSGLGIEKYITQFEKDHDDYNSILLKALADRLAEAFAEYLHHEVRTNIWAYAENENFSNEELIKEKYAGIRPAPGYPACPDHTEKIGLFELLNVSESIGISLTDSLAMMPASSVSGWYFAHPDSKYFGLGKVNEEQIRDISQRKGLDFEVNKKW 883          

HSP 2 Score: 255.373 bits (651), Expect = 1.284e-67
Identity = 120/154 (77.92%), Postives = 137/154 (88.96%), Query Frame = 0

Query:  308 KTNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSND 461
            ++NFVNIGERTNVTGSR FLRLI+   ++ A+ VA +QV GGAQI+D+NMDEGM+DGKEAMV FLNLIA+EPDIAR+P+MIDSSKWEIIEAGLKCIQGKG+VNSISLKEGE +FI QA  +K +GAA IVMAFDE GQADSYERRIEICKRS D
Sbjct:   19 ESNFVNIGERTNVTGSRAFLRLIQSGDYESAVAVALEQVNGGAQIIDINMDEGMIDGKEAMVTFLNLIAAEPDIARVPIMIDSSKWEIIEAGLKCIQGKGIVNSISLKEGETQFITQANTIKNFGAAVIVMAFDEQGQADSYERRIEICKRSYD 172          

BLAST of methionine synthase isoform 3 vs. nr
Match: gi|1247526921|gb|PCJ82431.1| (methionine synthase [Flavobacteriales bacterium])

HSP 1 Score: 818.15 bits (2112), Expect = 0.000e+0
Identity = 393/611 (64.32%), Postives = 484/611 (79.21%), Query Frame = 0

Query:  447 DSYERRIEICKRSNDNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKEFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLNP 1057
            D  E   ++     ++ATE LLE+A++VKG+ + +E+   WR   V+ R++H+LVKGIT++I EDTEE R    +P+ VIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMK+AVAYL P++E  K G     GKVL+ATVKGDVHDIGKNIVGVVLGCNNY+I+D+GVMVP+Q I++KA EENVD+IGLSGLITPSLDEMV VA+E+E+ G   PLLIGGATTSRVHTAVKIE+NYK  QTIHVLDASRSV VV KLLGN   +F  +I+ EY KVR NHAKKK  K  ++   A EN+ +IDWK E+V+ P F G KTF+ + L E+  +IDWTPFFQTW+LAGR+P+IL D+IVGE+A  L+ +A  ML KI  EKWL A+ VIG+WPANS  +DD+ I+ N  R +V  T ++LRQQ++KA    N +L+DF+APK+T L DYIGGFVVTAG+GIE+H++ F+K +DDY++ILLKALADRLAEAFAELMHKKVR E W Y   E+  NE+LIKE+Y+GIRPAPGYPACPDHTEK  L+K L+A K   V+LTES+AMYPA+SVSGFY+AHP+SKYFGLGKI  DQV+D AKRKGM   + ++WL P
Sbjct:  607 DLLEHVEDVLLNRREDATERLLEFAQTVKGEVQAEEKLQEWRNASVEERLSHSLVKGITDYIEEDTEEARQKSEKPLHVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPYLEAEKGGGRLSAGKVLLATVKGDVHDIGKNIVGVVLGCNNYEIIDLGVMVPSQTILQKAKEENVDIIGLSGLITPSLDEMVHVAKELEREGFSTPLLIGGATTSRVHTAVKIEQNYKRAQTIHVLDASRSVPVVGKLLGNKKVDFEKEIRAEYAKVRENHAKKKAAKEFLSIGQARENKFQIDWKTEDVFHPKFEGIKTFDEYPLEEIRNYIDWTPFFQTWELAGRFPKILEDKIVGEEAQKLYDEANAMLDKIVAEKWLKARAVIGLWPANSI-EDDIEIYANGSRKDVKITLHTLRQQSKKAARAANIALSDFVAPKQTELNDYIGGFVVTAGIGIEKHIERFEKDYDDYNSILLKALADRLAEAFAELMHKKVRREYWSYGAKESFSNEQLIKEEYKGIRPAPGYPACPDHTEKWQLWKLLDAEKNAAVKLTESLAMYPASSVSGFYFAHPESKYFGLGKIGKDQVEDYAKRKGMSIEETERWLGP 1216          

HSP 2 Score: 713.375 bits (1840), Expect = 0.000e+0
Identity = 339/492 (68.90%), Postives = 404/492 (82.11%), Query Frame = 0

Query:    1 LDGAMGTMIQQYKLGEDDFRKGWFEDSTFPLKGNNDLLSLTRPDIIKAIHAKYFEAGADIVETNTFSGTTIAQEDYGTEDKVYEINYHSAKIAREVADEFTAKEPNKPRFVAGSIGPTNKTASISPVINDPGYRAISFDELVTAFKLQVEALVEGGVDMLLVETVIDTLNAKAALFAIEQVFQEKDIELPIMLSGTITDQSGRTLSGQTTEAFLISTEHAPITSIGLNCALGAKDLRPYLQVMAKKSPFLVSAHPNAGLPNEFGEYDETPEMMAEQIKVFLDEGLLNIVGGCCGTSPAHIKAIAEL---------------------------AKTNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRSNDNATE 465
            LDGAMGTMIQ+Y L E+DFR   F+D   PL+GNNDLLS+TRPD+IK IH+ YFEAG+D+VETNTFS T+IAQ DY  E+  YE+NY SAKIA+E+A+EFT   P KPRFVAG++GPTNKTAS+SP +NDPGYRAI+FD+L  A+  Q + LVEGG D+LLVET+ DTLNAKAA+FAI+Q+F+EK IELPIM+SGTITD SGRTLSGQTTEAFLIS  H P+ S+GLNCALGAK+LRPY++ +AKKSPF VSA+PNAGLPNE GEYDE PE MA+Q++ F+  G +NIVGGCCGT+P HI+AIAE+                           A++NFVNIGERTNVTGSRKFLRLIKEE ++EAL VARDQVEGGAQ++DVNMDEG+LD  EAM KFLNLIA+EPDIA++P+MIDSSKWE+IE GLKCIQGKG+VNSIS+KEGEEKFI QAK V+ YGAA IVMAFDE GQAD+YE+RI+IC+R+    TE
Sbjct:   15 LDGAMGTMIQRYNLSEEDFRGERFKDHPCPLQGNNDLLSITRPDVIKEIHSAYFEAGSDMVETNTFSSTSIAQADYQLEELAYELNYQSAKIAKEIAEEFTINNPEKPRFVAGALGPTNKTASLSPAVNDPGYRAITFDQLKDAYYEQAKGLVEGGADVLLVETIFDTLNAKAAIFAIDQLFEEKGIELPIMISGTITDASGRTLSGQTTEAFLISVSHIPLLSVGLNCALGAKELRPYIETLAKKSPFFVSAYPNAGLPNEMGEYDELPEDMAQQLEDFIGSGFVNIVGGCCGTTPEHIQAIAEVAAKFPPREIPEIEAIPQFSGMEPVHVYAESNFVNIGERTNVTGSRKFLRLIKEENYEEALSVARDQVEGGAQLIDVNMDEGLLDANEAMPKFLNLIAAEPDIAKLPIMIDSSKWEVIEEGLKCIQGKGIVNSISMKEGEEKFISQAKLVRRYGAAVIVMAFDEKGQADTYEKRIQICERAYKILTE 506          

BLAST of methionine synthase isoform 3 vs. nr
Match: gi|503451177|ref|WP_013685838.1| (methionine synthase [Fluviicola taffensis] >gi|327318574|gb|AEA43066.1| methionine synthase (B12-dependent) [Fluviicola taffensis DSM 16823])

HSP 1 Score: 809.675 bits (2090), Expect = 0.000e+0
Identity = 388/607 (63.92%), Postives = 479/607 (78.91%), Query Frame = 0

Query:  450 ERRIEICKRSNDNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKEFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWLN 1056
            ER  ++     D++TE LL++AE+ KG+ KKKE  L WR   V  R+ HALVKGI +FI ED EECR  +  PI+VIEGPLMDGMN+VGDLFGSGKMFLPQVVKSARVMK+AVAYL PFI+ AK+G+    GKV+MATVKGDVHDIGKNIV VVL CNNY+IVD+GVMVP + II+ AI+E  DVIGLSGLITPSLDEMV +A+EME+A L IPLLIGGATTS+VHTAVKIE+NY  GQT+HVLDASRSVTVVE+LLG   ++FV DI+ +Y ++R +H K +  K++++Y  A++N+L +++  E +  P  +G +      L+ L+P+IDWTPFFQTW+L G++PRILTDE+VG++AT L+ADAQ ML +I  EKWLTAK   GI+P NS   DD+ I+++E RT V    +SLRQQT+KA   PN SLADFIAPKE+GL DY+G FVVTAG GIEEHVK F+  HDDY++I++KALADR AEAFAE +H KVR E WGYA +ETLDN+ LI EKY GIRPAPGYPACPDHTEK  LFK LNA +  GV LTE++AM+PAASVSG+Y+AHP SKYFGLGK+ +DQV  I+ RK M+   MK+WL+
Sbjct:  278 ERVEDVLLNRRDDSTERLLDFAETFKGEDKKKEVALEWRNAPVNERLAHALVKGIVDFIDEDVEECRHQFERPIQVIEGPLMDGMNIVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPFIDAAKDGKSSSAGKVIMATVKGDVHDIGKNIVSVVLSCNNYEIVDLGVMVPAERIIQAAIDEKADVIGLSGLITPSLDEMVHMAKEMERANLSIPLLIGGATTSKVHTAVKIEQNYTKGQTVHVLDASRSVTVVEQLLGYKKEQFVKDIQADYARLREHHEKHRGAKQVLSYADALKNKLVLEFNGETIKKPKQLGVQVIEEQDLALLVPYIDWTPFFQTWELHGKFPRILTDEVVGKEATQLYADAQAMLKQIVDEKWLTAKAAFGIFPVNSI-DDDIEIYSDEQRTSVLGIQHSLRQQTKKAAGQPNLSLADFIAPKESGLIDYLGAFVVTAGHGIEEHVKRFEADHDDYNSIMIKALADRFAEAFAEFLHAKVRRETWGYANDETLDNDALIAEKYIGIRPAPGYPACPDHTEKPNLFKLLNAEELSGVSLTETLAMWPAASVSGWYFAHPDSKYFGLGKVTLDQVDSISTRKNMDINHMKRWLS 883          

HSP 2 Score: 265.003 bits (676), Expect = 6.095e-71
Identity = 121/160 (75.62%), Postives = 146/160 (91.25%), Query Frame = 0

Query:  300 IKAIAELAKTNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRS 459
            ++AI    ++NF NIGERTNVTGSR FLR+IK+  ++ AL VAR+QVEGGAQI+D+NMDEGM+DGKEAMVKFLNLIA+EPDI+++P+M+DSSKWEIIEAGL+CIQGKG+VNSISLKEGEE FI QAK +K YGAA+++MAFDEDGQADSYERRIEIC+RS
Sbjct:    9 LEAITITPESNFTNIGERTNVTGSRAFLRMIKDNDYEAALSVAREQVEGGAQIIDINMDEGMIDGKEAMVKFLNLIAAEPDISKVPIMVDSSKWEIIEAGLQCIQGKGIVNSISLKEGEENFIRQAKLIKRYGAASVIMAFDEDGQADSYERRIEICERS 168          

BLAST of methionine synthase isoform 3 vs. nr
Match: gi|1085078391|gb|OGS77561.1| (methionine synthase [Candidatus Fluviicola riflensis] >gi|1085085256|gb|OGS84142.1| methionine synthase [Fluviicola sp. RIFCSPHIGHO2_12_FULL_43_24] >gi|1085085742|gb|OGS84627.1| methionine synthase [Fluviicola sp. RIFCSPHIGHO2_01_FULL_43_53] >gi|1229049053|gb|ASS50132.1| methionine synthase [Candidatus Fluviicola riflensis])

HSP 1 Score: 803.512 bits (2074), Expect = 0.000e+0
Identity = 396/601 (65.89%), Postives = 485/601 (80.70%), Query Frame = 0

Query:  455 ICKRSNDNATEALLEYAESVKGDGKKKERDLTWRETDVKGRITHALVKGITEFIVEDTEECRLLYPEPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAYLNPFIEEAKNGEVKPNGKVLMATVKGDVHDIGKNIVGVVLGCNNYDIVDMGVMVPTQDIIKKAIEENVDVIGLSGLITPSLDEMVSVAEEMEKAGLKIPLLIGGATTSRVHTAVKIEENYKSGQTIHVLDASRSVTVVEKLLGNTSKEFVADIKTEYEKVRVNHAKKKVQKRLVAYDMAIENRLKIDWKAEEVYTPNFIGNKTFNNFSLSELIPFIDWTPFFQTWQLAGRYPRILTDEIVGEQATSLFADAQEMLAKIEKEKWLTAKGVIGIWPANSNGKDDVSIFTNEDRTEVDETFYSLRQQTQKAKNLPNFSLADFIAPKETGLQDYIGGFVVTAGLGIEEHVKEFQKTHDDYSAILLKALADRLAEAFAELMHKKVREELWGYAPNETLDNEELIKEKYQGIRPAPGYPACPDHTEKLTLFKKLNATKEIGVELTESMAMYPAASVSGFYYAHPQSKYFGLGKIEMDQVKDIAKRKGMEEAKMKKWL 1055
            I  R  D ATE LL++AES KG+ KKKE +L WR   V  R++HALVKGI ++I ED EECR  +  PIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMK+AVAYL PFIEE K G+ +  G+++MATVKGDVHDIGKNIVGVVLGCNNY+++D+GVMVP   II+ AIE    VIGLSGLITPSLDEMV VA+EME+A L IPLLIGGATTS+VHTAVKIE++Y+ GQT+HVLDASRSVTVVE+LLG+   +FV D+K EY ++R +H K +  K+L++   A  N+L++ +    V  P  +G +TF +F L +L+ +IDWTPFFQTW+L GRYP ILTDE+VG QAT LFADAQ ML K+  EKWL AK V+GI+PANS G DD+ I+ +E R+EV    ++LRQQT K    PN +L+DFIAPKE+G++DYIGGFVVT G GI+EHV+ F+  HDDYS+ILLKALADRLAEAFAE MH  VR++ WGYAP+ETLDN ELI+EKY+GIRPAPGYPACPDHTEK  LF+ LNAT+  GV LTE++AM+PA+SVSG+Y+AHP SKYFGLGKI  +QV+DIA+RK ++ A+M++WL
Sbjct:  285 ILNRRPD-ATERLLDFAESFKGEEKKKEVELEWRSALVNERLSHALVKGIVDYIDEDVEECRHQFKRPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLLPFIEEEKGGKSETAGRIIMATVKGDVHDIGKNIVGVVLGCNNYEVIDLGVMVPADKIIQAAIEHKAHVIGLSGLITPSLDEMVYVAKEMERAQLDIPLLIGGATTSKVHTAVKIEQSYERGQTVHVLDASRSVTVVEQLLGHKKGQFVEDLKLEYTRLREHHEKHRGAKQLLSLAAAQANKLQLTFDESTVSAPKELGTQTFEHFPLEKLVDYIDWTPFFQTWELHGRYPNILTDEVVGAQATELFADAQAMLKKLIDEKWLVAKAVVGIFPANSVG-DDIEIYADETRSEVINIQHTLRQQTTKVSGQPNLALSDFIAPKESGVKDYIGGFVVTTGHGIDEHVQRFEDDHDDYSSILLKALADRLAEAFAEYMHAYVRKQKWGYAPDETLDNSELIQEKYRGIRPAPGYPACPDHTEKPGLFRILNATEVSGVSLTETLAMWPASSVSGWYFAHPDSKYFGLGKITNEQVEDIARRKHIDLAEMRRWL 883          

HSP 2 Score: 269.626 bits (688), Expect = 1.150e-72
Identity = 126/160 (78.75%), Postives = 146/160 (91.25%), Query Frame = 0

Query:  300 IKAIAELAKTNFVNIGERTNVTGSRKFLRLIKEEKFDEALDVARDQVEGGAQILDVNMDEGMLDGKEAMVKFLNLIASEPDIARIPVMIDSSKWEIIEAGLKCIQGKGVVNSISLKEGEEKFIEQAKKVKMYGAATIVMAFDEDGQADSYERRIEICKRS 459
            ++AI    ++NF+NIGERTNVTGSR FLR+I++  F+ AL VAR+QVEGGAQI+D+NMDEGM+DGKEAMVKFLNLIA+EPDIAR+PVMIDSSKWEIIEAGLKCIQGKG+VNSISLKEGEE FI QAKK+K +GAA +VMAFDE GQADSYERRIEIC+RS
Sbjct:   10 LEAITVTPESNFINIGERTNVTGSRAFLRMIRDNDFEAALSVAREQVEGGAQIIDINMDEGMIDGKEAMVKFLNLIAAEPDIARVPVMIDSSKWEIIEAGLKCIQGKGIVNSISLKEGEENFIAQAKKIKRFGAAVVVMAFDEKGQADSYERRIEICERS 169