hypothetical protein DAPPUDRAFT_300683, snap_masked-scaffold1086_size63525-processed-gene-0.0 (gene) Tigriopus kingsejongensis

Overview
Namehypothetical protein DAPPUDRAFT_300683
Unique Namesnap_masked-scaffold1086_size63525-processed-gene-0.0
Typegene
OrganismTigriopus kingsejongensis (Tigriopus kingsejongensis)
Associated RNAi Experiments

Nothing found

Homology
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Match: EMLSAG00000009160 (supercontig:LSalAtl2s:LSalAtl2s577:434769:489672:1 gene:EMLSAG00000009160 transcript:EMLSAT00000009160 description:"maker-LSalAtl2s577-augustus-gene-4.7")

HSP 1 Score: 315.079 bits (806), Expect = 4.257e-94
Identity = 256/730 (35.07%), Postives = 363/730 (49.73%), Query Frame = 0
Query:  219 LDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSL------------------------PLDTFHKMTRLQHLYLQ---DNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLR-------RIRVLDLSHNCISHIEDPTFGA-ANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQ 913
            L+L++  I EL    L+ L NSL+ LDLS N L  +PE IF D   +  L L+ N +    ++ F      + ++ L G  +  + L+E+ +            N  G +    F +   S+    L  + + SIE + F ++  I  + L +  +E +   AF+ +  +L+ L +S A   + +P+  F     LR L LS NH+ S                         PL+ +  +  L+ L +    D +    K  TF +   P L ILDL     + V YDTFRF +LQ LLL+DN IKK+D +SFVEMT L+ L+L GN I  L DETFQNLH L+ LD+                          S+N L+ L     ++  TLSN + +D+S+N +       +     DL+        I+E+   +             I  LDLS+N  + +    F +  N L  L L+ N L  L G  +   ++L+++DLSHN +  L   +F         +A  RL                       Q+ ++ +SQL+QI LLV L+L  N IS ID  AFCC+PNL  LNL  NP+  ++ D F G+++ L+ LN+AN SLTLLP  QL  LK LNVS NQLTF+P N LANLSQV +LDLS NELTSPPS+AW  M HL  L L GNPI +V+NDSF GL  LESLDIS +  + +Q
Sbjct:   37 LNLADNNISELLREDLKSLTNSLKTLDLSGNCLTSVPEGIFWDLQRLVSLDLSRNNIIQIHSMAFQNGVPLLAEIYLSGNLLGSLKLDELNL----------DYNYLGPLKAGSFENFE-SINYTRLQGNPIGSIEEDTFSNI-KIKYLFLRDASLEELLPKAFKGLEKSLEVLDLS-ANSLKEIPDDIFEDHDYLRKLVLSENHLVSFSPNASGCRFNVVDLDLIGPDNGFTPLEEYGLVQTLRVLGMSRIGDEKSGDLKSETFEAFV-PGLEILDLR----KHVPYDTFRFAELQHLLLSDNLIKKIDGKSFVEMTDLIDLNLAGNAIEGLEDETFQNLHSLENLDI--------------------------SYNHLKTLNFRAFDSLGTLSN-LRIDVSHNALGEEGQELY-----DLR----KXGXIAEVPWSEASSSSSLSSSSSNIASLDLSYNNFTALRPGYFYSLQNGLKVLNLSCNSLEYLDG--ISGLRQLRILDLSHNKICELDPRVF---------IASERL-----------------------QMLSLRNSQLSQIHLLVTLDLSRNLISIIDDKAFCCLPNLVYLNLGRNPIKTLNADLFHGVKNTLETLNIANASLTLLPKFQLHKLKHLNVSGNQLTFVPLNNLANLSQVNKLDLSYNELTSPPSSAWQYMHHLNELSLRGNPISKVLNDSFVGLTRLESLDISRIHPEEYQ 678          

HSP 2 Score: 233.032 bits (593), Expect = 9.123e-65
Identity = 215/721 (29.82%), Postives = 347/721 (48.13%), Query Frame = 0
Query:   67 SLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFF----------------IGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDN-MLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNN--YGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRL----------------QHLY---------------------------------LQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVN----RSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQ-NDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEEL-FLRTSLEIFKV 713
             L  +L EL+L  N LT +PE     L++L RL+L+ NNI ++     ++    L  LDL+ N +  +P      ++ L  LDLS N I ++    F                +G L LD L L  N +  L A +F+NFE INYT L GNP+  IE D F   +IK L L +  ++EL   A +GLE SLE LDLSAN L+E+P+DIF D D +R+L L++N ++ FSPN +  G R+ + DL+L+G D  + PLEE G+++ LR +G+S + +   G + ++ F    P LE L+L       + ++ F+    +  + LS+N I+ I+  +F E+ + +      NA+    L +  F++L +L  LD+S NH+++L    F  +  L                Q LY                                 L  N  +  + G F+S  N  L +L+LS N +E ++       +L+ L L+ N+I +LD + F+   RL +LSL  +Q+ Q        +H L  LDL+ N +  ++  AF  +  L +L  +L  N ++ L  +      NT  TL           NI+   +T     Q + LK LN+S N ++ +  +++  L ++  LDLS+N ++      +   + L+ L L  N ++ +         RL+ +D+S       PEE  FLR+     +V
Sbjct:    5 GLERALWELHLQHNRLTKIPEESISLLKKLTRLNLADNNISELLREDLKSLTNSLKTLDLSGNCLTSVPEGIFWDLQRLVSLDLSRNNIIQIHSMAFQNGVPLLAEIYLSGNLLGSLKLDELNLDYNYLGPLKAGSFENFESINYTRLQGNPIGSIEEDTFSNIKIKYLFLRDASLEELLPKAFKGLEKSLEVLDLSANSLKEIPDDIFEDHDYLRKLVLSENHLVSFSPNAS--GCRFNVVDLDLIGPDNGFTPLEEYGLVQTLRVLGMSRIGDEKSGDLKSETFEAFVPGLEILDLR----KHVPYDTFRFA-ELQHLLLSDNLIKKIDGKSFVEMTDLIDLNLAGNAIE--GLEDETFQNLHSLENLDISYNHLKTLNFRAFDSLGTLSNLRIDVSHNALGEEGQELYDLRKXGXIAEVPWSEASSSSSLSSSSSNIASLDLSYNNFTALRPGYFYSLQN-GLKVLNLSCNSLEYLD-GISGLRQLRILDLSHNKICELDPRVFIASERLQMLSLRNSQLSQ--------IHLLVTLDLSRNLISIIDDKAFCCLPNLVYL--NLGRNPIKTLNADLFHGVKNTLETL-----------NIANASLTLLPKFQLHKLKHLNVSGNQLTFVPLNNLANLSQVNKLDLSYNELTSPPSSAWQYMHHLNELSLRGNPISKVLNDSFVGLTRLESLDISR----IHPEEYQFLRSKTLTLRV 689          

HSP 3 Score: 93.9745 bits (232), Expect = 8.854e-20
Identity = 158/589 (26.83%), Postives = 250/589 (42.44%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAV-LDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVAD-------PFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLEN-------SLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMY---------YVPLEEI-------GIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEH-NAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQ---------ANPSLAI-----------LDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLA------YNRLES--------------LNFAAFDSIGTLSHLAIDLS 567
            L D  + EL  +  + L  SL  L+L+ N+L  +P+ IF D   L +L LS N+++     A  +G     V LDL     G  P      V++LRVL +S    EK  D        F  G   L+ L LR    +++  + F+ F  + +  L+ N ++ I+  +F+E    L+DL+      LA NA+ GLE+       SLE LD+S N L+ L    F    T+  LR+         +++ N        L   GQ++Y          VP  E            N+ ++ L S NN+ ++    F  L   L+ LNL      S+E+ +    +  +  +DLS+N+I  ++   F            S  L   +L N     +  L TLDLS N I  +    F  +  L +L L  N I T     FH           AN SL +           L++S N +  V  +      ++ +L L+ N +    S ++  M  L  LSL GN I ++ +++F  L RL+ LD++      Y  L S              LN   FD++G++ +L +DL+
Sbjct:  175 LRDASLEELLPKAFKGLEKSLEVLDLSANSLKEIPDDIFEDHDYLRKLVLSENHLVSFSPNA--SGCRFNVVDLDLIGPDNGFTPLEEYGLVQTLRVLGMSRIGDEKSGDLKSETFEAFVPG---LEILDLR----KHVPYDTFR-FAELQHLLLSDNLIKKIDGKSFVE-MTDLIDLN------LAGNAIEGLEDETFQNLHSLENLDISYNHLKTLNFRAFDSLGTLSNLRI---------DVSHNA-------LGEEGQELYDLRKXGXIAEVPWSEASSSSSLSSSSSNIASLDL-SYNNFTALRPGYFYSLQNGLKVLNL---SCNSLEYLDGISGLRQLRILDLSHNKICELDPRVF----------IASERLQMLSLRNSQLSQIHLLVTLDLSRNLISIIDDKAFCCLPNLVYLNLGRNPIKTLNADLFHGVKNTLETLNIANASLTLLPKFQLHKLKHLNVSGNQLTFVPLNNLANLSQVNKLDLSYNELTSPPSSAWQYMHHLNELSLRGNPISKVLNDSFVGLTRLESLDISRIHPEEYQFLRSKTLTLRVFGRESTTLNSDLFDNLGSVQNLTLDLT 716          

HSP 4 Score: 80.8777 bits (198), Expect = 1.007e-15
Identity = 97/380 (25.53%), Postives = 160/380 (42.11%), Query Frame = 0
Query:  566 LSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFG----------------AANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFG-----------GIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRASD 918
            L HN+L  +      +   L  +  L+L+ NNIS +     + + N LK L+LS N ++ +       L+R+  LDLS N I  I    F                  + KL  L L++N L  L      N + +    L  N + ++ E+ F    ++   +    L E+  KA   ++ SL+ LDLSAN +  I D        L  L L  N + +   +A  C  N+  L+L         L+ +G            I D   G   + T    +P L++  L+  +V ++   F         ++++ L LS N +      ++  M  L+ L+L+GN I  + +++F  L  LE+LDIS    K+   RA D
Sbjct:   15 LQHNRLTKIP---EESISLLKKLTRLNLADNNISELLREDLKSLTNSLKTLDLSGNCLTSVPEGIFWDLQRLVSLDLSRNNIIQIHSMAFQNGVPLLAEIYLSGNLLGSLKLDELNLDYNYLGPLKAGSFENFESINYTRLQGNPIGSIEEDTFSNIKIKYLFLRDASLEELLPKAFKGLEKSLEVLDLSANSLKEIPDDIFEDHDYLRKLVLSENHLVSFSPNASGCRFNVVDLDLIGPDNGFTPLEEYGLVQTLRVLGMSRIGDEKSGDLKSETFEAFVPGLEILDLRK-HVPYDTFRF---------AELQHLLLSDNLIKKIDGKSFVEMTDLIDLNLAGNAIEGLEDETFQNLHSLENLDISYNHLKTLNFRAFD 381          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Match: EMLSAG00000009585 (supercontig:LSalAtl2s:LSalAtl2s616:5915:14813:-1 gene:EMLSAG00000009585 transcript:EMLSAT00000009585 description:"maker-LSalAtl2s616-snap-gene-0.5")

HSP 1 Score: 311.997 bits (798), Expect = 2.732e-90
Identity = 259/866 (29.91%), Postives = 425/866 (49.08%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALH-FRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSH--LAIDLSHNQLQALR--VNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDD--VGQLR--RIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLA---NLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDI 904
            L  N+IS++   +L +L  S+  L L GN L  +P   F   + L  L+L  N+I  I    F +    L  L L+ NL+ K P  +IA++ SL+   +  N IE++    F    HLD L + EN I +L  N F     +   +L  N +E I P  F     + + L    I+ +   A   +E+ ++ +DL +N+L ELP  +            N+ + +      F+ F  ++   +  G  +   P   +   + LR + L     Y      +F   +  L+ L L ++ LT +  + F+    +  + LS N  + ++  AF ++G  LQ L +S  L+  +A P+   R L  L  L   NN + S    + +    LQ+L L++N +    R   H   + SL  + L+ N I  ++  TF    +LQ + L+ N I  L+S  F  +  L  L L  N I Q+  +TF NL  LQ+LD++YN+L    F +FD+     +  L +DLSHNQ+  ++  V      P +S    LD+SYN ++ +    F+ + + LKILNLS N IS I  DD  +  L   +++VL+LSHN +  ++   F     +  LYLN N L  L          L+++DL  N L +LP ++F  +S+E   ++ N    +P  ALN + SSL+YL+LS N+I  +  +  +    L+ L+L  NK++ +  + F  + +L  L+L+ N + R +L         L+ L +A + L  LP+  L SL  L+VS N L+ +P N L     L +++ L+LSGN+L   P+ +W+ +  L SL +S NP+  +  +SF GL  L+ L +
Sbjct:  192 LYGNEISDIGTSNLLNLKASVSTLFLGGNDLKDIPPYFFNTFKNLTWLNLDHNHIFHIPDTCFPST---LITLSLSYNLINKFPSGAIANMPSLKWFTIRGNYIEQIPQIRFGSTKHLDKLDIGENFIVSLPENMFNGSVDVIDLNLDYNYVERIGPRVFKSLNARRIYLGFNRIEFIDDEAFEDVEDMIDLIDLESNKLLELPSALGRLRRLRYLYLQNNYISKIESPKIFDSFCSSLLAFSAAGNGLTEFPYTTLHNCKVLRHLNLGYNKIYH--IKPEFFVWASKLDTLILRNNRLTQLGPHVFRDCTKLRELSLSFNDFQVLDSEAFTDIGTTLQSLEISFGLNGIKAFPHNALRPLQKLMWLAFDNNELSSAEDTSLYNAGDLQYLNLENNNLHDLPRNLLHKNVHSSLIDVRLAHNRISIIKTGTFVSLKELQTIDLSGNSIVILESTGFFNLKNLFTLILSHNYINQIDPKTFSNLLNLQKLDMSYNKLTKFEFDSFDNCTKHVNNPLHLDLSHNQIDYIQEAVFSKTASPIIS---ELDVSYNILTRIPKEIFKSIVDHLKILNLSFNQISRIRKDDFIISTLNALKVQVLNLSHNNLRDLDKRCFDTLTGIQILYLNDNALEMLQFAQFSGLTGLRILDLHKNKLRSLPRDVFQFSSVETLDLSWNEFVALPTSALNEISSSLRYLNLSHNKIEHLDSTMFSNFPHLLSLSLASNKLTILPDNIFSGLGSLIILDLSYNAI-RANLRELFHYVHRLRELRLAGSFLRELPSFTLPSLVKLDVSDNHLSDLPYNYLTVEDGLYRLKYLNLSGNDLIRLPARSWYYVPFLKSLDVSWNPLRVLTKESFYGLARLQILSV 1048          

HSP 2 Score: 167.548 bits (423), Expect = 1.782e-42
Identity = 229/885 (25.88%), Postives = 392/885 (44.29%), Query Frame = 0
Query:   72 LRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIG-QLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIG--IMRNL--RTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNG----PFRSL---SALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLL---LNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFK---VAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISD-----SQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGI-----------------RDHLQ-----GLNVANTSLTLLPNLQL----SSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISG 906
            L  +++    L  V    F+    +E + L +N I  I S AF+T    L  LDL+ N + KIP  + + +  L+ L+L  N I  +     +  +  + TL L  N+++++    F  F+ + + +L  N +  I PD    + +  L LS  LI +  S A+  +  SL+   +  N ++++P+  F     + +L + +N +   P   FNG    + DLNL   D  YV  E IG  + ++L  R + L   N    +  + F D+   ++ ++L  + L  +  +A   +  +  + L NN I  IE     +          S+ L F A  NG    P+ +L     LR L+L  N I  +  + F   ++L  L L++N ++      F       L  L LSFN  + ++ + F   G   Q L      N IK     +   + +L+ L+ + N++    D +  N   LQ L+L  N L  L           S + + L+HN++  ++   + T+ +L  + ++DLS N+I  +E T F  ++N L  L LS N I++I P     L  ++ LD+S+N ++  E  +F    K    ++N+     LH            +DLSHN ++ + E +F +T+  I     V++N L  IP +    +   LK L+LS NQI+ I       S LN +++ V LNL  N +  +D   F  +  ++ L L  N L  +    F G+                 RD  Q      L+++      LP   L    SSL+ LN+S N++  +     +N   +  L L+ N+LT  P   +  +  L+ L LS N I   + + F  +  L  L ++G
Sbjct:  114 LSSIDIIDGNLEIVESSAFFSSTSIESVRLMKNKIRLIESDAFRTLATTLKSLDLSYNSLDKIPENAFSRLTVLKWLNLYGNEISDIGTSNLLNLKASVSTLFLGGNDLKDIPPYFFNTFKNLTWLNLDHNHIFHI-PDTCFPSTLITLSLSYNLINKFPSGAIANMP-SLKWFTIRGNYIEQIPQIRFGSTKHLDKLDIGENFIVSLPENMFNG-SVDVIDLNL---DYNYV--ERIGPRVFKSLNARRIYLG-FNRIEFIDDEAFEDVEDMIDLIDLESNKLLELP-SALGRLRRLRYLYLQNNYISKIESPKIFDSF-------CSSLLAFSAAGNGLTEFPYTTLHNCKVLRHLNLGYNKIYHIKPEFFVWASKLDTLILRNNRLTQLGPHVFRDCT--KLRELSLSFNDFQVLDSEAFTDIGTTLQSLEISFGLNGIKAFPHNALRPLQKLMWLAFDNNELSSAEDTSLYNAGDLQYLNLENNNLHDLPRNLLHKNVHSSLIDVRLAHNRISIIK---TGTFVSLKELQTIDLSGNSIVILESTGFFNLKN-LFTLILSHNYINQIDPKTFSNLLNLQKLDMSYNKLTKFEFDSFDNCTK----HVNN----PLH------------LDLSHNQIDYIQEAVFSKTASPIISELDVSYNILTRIPKEIFKSIVDHLKILNLSFNQISRIRKDDFIISTLNALKVQV-LNLSHNNLRDLDKRCFDTLTGIQILYLNDNALEMLQFAQFSGLTGLRILDLHKNKLRSLPRDVFQFSSVETLDLSWNEFVALPTSALNEISSSLRYLNLSHNKIEHLDSTMFSNFPHLLSLSLASNKLTILPDNIFSGLGSLIILDLSYNAIRANLRELFHYVHRLRELRLAG 954          

HSP 3 Score: 151.369 bits (381), Expect = 1.703e-37
Identity = 176/681 (25.84%), Postives = 302/681 (44.35%), Query Frame = 0
Query:  302 YVPLEEIGIMRNLRTVG--LSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMS-NALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKM-TRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQAL--RVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVG--------------------------------------------------QLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELF--LRTSLEIFKVAH--NRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHR-IHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRASD-CS 920
            Y P E I   R++R VG  L+ + NY            P L +++++D +L  +E +AF    SI S+ L  N+I  IE +AFR +   L+ L +S N+L    +P   F  L+ L+ L+L  N I  +       +   +  L+L  N++       F++  N  L  L+L  NHI  +  DT     L  L L+ N I K  S +   M  L   ++ GN I Q+P   F +   L +LD+  N + SL    F+  G++  + ++L +N ++ +  RV +S       N   + L +N I F++   FE V++ + +++L  N + E+ P  +G                                                    + +R L+L +N I HI+   F  A+KL +L L +NRLT L   + R+  +L+ + LS N  + L  E F  + T+L+  +++   N +   P  AL P+Q  L +L    N++++  D+ L     L YLNLE N +                 +L  N LH+ +H                             SSL  + ++ N+++ I   T  +L +++ +DLSGN +    ST + ++ +L +L LS N I ++   +F+ L +L+ LD+S  +   F+  + D C+
Sbjct:   87 YSPEEYI---RDIRCVGIPLAKIPNYPI----------PFLSSIDIIDGNLEIVESSAFFSSTSIESVRLMKNKIRLIESDAFRTLATTLKSLDLSYNSLD--KIPENAFSRLTVLKWLNLYGNEISDIGTSNLLNLKASVSTLFLGGNDLKDIPPYFFNTFKN--LTWLNLDHNHIFHIP-DTCFPSTLITLSLSYNLINKFPSGAIANMPSLKWFTIRGNYIEQIPQIRFGSTKHLDKLDIGENFIVSLPENMFN--GSVDVIDLNLDYNYVERIGPRVFKS------LNARRIYLGFNRIEFIDDEAFEDVEDMIDLIDLESNKLLEL-PSALGRLRRLRYLYLQNNYISKIESPKIFDSFCSSLLAFSAAGNGLTEFPYTTLHNCKVLRHLNLGYNKIYHIKPEFFVWASKLDTLILRNNRLTQLGPHVFRDCTKLRELSLSFNDFQVLDSEAFTDIGTTLQSLEISFGLNGIKAFPHNALRPLQK-LMWLAFDNNELSSAEDTSLYNAGDLQYLNLENNNLH----------------DLPRNLLHKNVH-----------------------------SSLIDVRLAHNRISIIKTGTFVSLKELQTIDLSGNSIVILESTGFFNLKNLFTLILSHNYINQIDPKTFSNLLNLQKLDMSYNKLTKFEFDSFDNCT 694          

HSP 4 Score: 126.331 bits (316), Expect = 1.232e-29
Identity = 207/863 (23.99%), Postives = 364/863 (42.18%), Query Frame = 0
Query:  135 PFISIAHVKSL----RVLDLSNNRIEKVADPFFIGQLHL---DTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIE--PDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDF-DTIRRLRLNDNMLQFSPNITFNGFRYTI-KDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLS--FNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLL-ILSLEGNQIRQLPDE--TFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSN---IMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFG---------------------------AANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELF---LRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQG-----------------------------LNVANTSLTLLPNLQLSS----LKSLNVSFNQLTFIPPNTLA----NLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKS 911
            PF  +   KS+     +L+    R     +P F GQ  +   + + LR+ + +   ++ +   EYI      G PL  I   P  FL +    +D+ +  ++ + S+A      S+E++ L  N+++ +  D F     T++ L L+ N L   P   F+  R T+ K LNL G ++      +IG                    T    +L  S+  L L  +DL  I    F    +++ ++L +N I +I +  F    + L  L +S  L     P+G   ++ +L+   +  N+I  +P   F     L  L + +N I +     F    N S+ ++DL+  +N++ER+    F+    +++ L  NRI+ +D ++F ++  ++ ++ LE N++ +LP      + L  L   +   +++ES     FDS  + S LA   + N L          Y TL N   +  L+L YN I  ++  FF    + L  L L  N ++++ P       ++R L LS N    ++   F                               KL  L  ++N L+S     L N   LQ ++L +N L  LP  L    + +SL   ++AHNR+  I       ++  L+ +DLS N I  +  +    ++ L  L L  N I+ ID   F  + NL++L+++ N L +   D+F     H+                               L+V+   LT +P     S    LK LN+SFNQ++ I  +       N  +V+ L+LS N L       + ++  +  L+L+ N +  +    F+GL  L  LD+   + +S
Sbjct:   28 PFNQLCSCKSVPHIPELLESQKRRSGDATEPTFHGQFGVFYENDMDLRKEDGKKKDSSVYSPEEYIRDIRCVGIPLAKIPNYPIPFLSS----IDIIDGNLEIVESSAFFS-STSIESVRLMKNKIRLIESDAFRTLATTLKSLDLSYNSLDKIPENAFS--RLTVLKWLNLYGNEI-----SDIG--------------------TSNLLNLKASVSTLFLGGNDLKDIPPYFFNTFKNLTWLNLDHNHIFHIPDTCF---PSTLITLSLSYNL-INKFPSGAIANMPSLKWFTIRGNYIEQIPQIRFGSTKHLDKLDIGENFIVSLPENMF----NGSVDVIDLNLDYNYVERIGPRVFKSLNARRIYLGFNRIEFIDDEAFEDVEDMIDLIDLESNKLLELPSALGRLRRLRYLYLQNNYISKIESPKI--FDSFCS-SLLAFSAAGNGLTEF------PYTTLHNCKVLRHLNLGYNKIYHIKPEFFV-WASKLDTLILRNNRLTQLGPHVFRDCTKLRELSLSFNDFQVLDSEAFTDIGTTLQSLEISFGLNGIKAFPHNALRPLQKLMWLAFDNNELSSAEDTSLYNAGDLQYLNLENNNLHDLPRNLLHKNVHSSLIDVRLAHNRISIIKTGTFVSLKE-LQTIDLSGNSIVILESTGFFNLKNLFTLILSHNYINQIDPKTFSNLLNLQKLDMSYNKLTKFEFDSFDNCTKHVNNPLHLDLSHNQIDYIQEAVFSKTASPIISELDVSYNILTRIPKEIFKSIVDHLKILNLSFNQISRIRKDDFIISTLNALKVQVLNLSHNNLRDLDKRCFDTLTGIQILYLNDNALEMLQFAQFSGLTGLRILDLHKNKLRS 839          

HSP 5 Score: 81.6481 bits (200), Expect = 6.832e-16
Identity = 117/437 (26.77%), Postives = 200/437 (45.77%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLE----RLDLSRNNIIQIHSMAF-QTGVPHLAVLDLADNLMGKIP---FISIAHVKSLRVLDLSNNRIEKV-ADPFFIGQLH---LDTLQLRENNIQNLTA----------------NAFQNFEYINYTSLAG--------NPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGF-RYTIKDLNL------LGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQD 445
            L  N I+++  +   +L L+L++L+++ N LT      F +  +       LDLS N I  I    F +T  P ++ LD++ N++ +IP   F SI  V  L++L+LS N+I ++  D F I  L+   +  L L  NN+++L                  NA +  ++  ++ L G        N L  +  D F  + ++ LDLS      L ++AL  + +SL  L+LS N+++ L   +F +F  +  L L  N L   P+  F+G     I DL+       L +  +YV       +R LR  G S +    S T        PSL  L++ D+ L+ + +N                    +E+  +R     L+YL +S     R LP   +  +  L++LD+S N +R L  ++F+ + RLQ L +++
Sbjct:  653 LSHNYINQIDPKTFSNL-LNLQKLDMSYNKLTKFEFDSFDNCTKHVNNPLHLDLSHNQIDYIQEAVFSKTASPIISELDVSYNILTRIPKEIFKSI--VDHLKILNLSFNQISRIRKDDFIISTLNALKVQVLNLSHNNLRDLDKRCFDTLTGIQILYLNDNALEMLQFAQFSGLTGLRILDLHKNKLRSLPRDVFQFSSVETLDLSWNEFVALPTSALNEISSSLRYLNLSHNKIEHLDSTMFSNFPHLLSLSLASNKLTILPDNIFSGLGSLIILDLSYNAIRANLRELFHYVHR-----LRELRLAG-SFLRELPSFTL-------PSLVKLDVSDNHLSDLPYNYLT-----------------VEDGLYR-----LKYLNLSGNDLIR-LPARSWYYVPFLKSLDVSWNPLRVLTKESFYGLARLQILSVRN 1050          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Match: EMLSAG00000009829 (supercontig:LSalAtl2s:LSalAtl2s63:1003125:1030351:1 gene:EMLSAG00000009829 transcript:EMLSAT00000009829 description:"maker-LSalAtl2s63-augustus-gene-10.41")

HSP 1 Score: 209.149 bits (531), Expect = 1.395e-55
Identity = 243/873 (27.84%), Postives = 412/873 (47.19%), Query Frame = 0
Query:   96 LERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKI--PFISIAHVK---SLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAF-----------QNFEYINYTSLAGN---------------PLEWIEPDAFLE-TQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMY-YVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRL-QHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDE-----------------------TFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRS-NTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALN-PVQSSLKYLDLSANQITTIS-DSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNE---LTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
            L+ LD S N I ++    F      L VL L +NL+G    P  S +  +   +LR LDLS+N +  + D  F G  +L  L++  N ++ +   A            Q+ +  N   L G+                +  I P  F   T++  L L+   +  + S+  + L   +E LDLS+N L  +P    +   T+R L L  N+++      F     +++ L+L   +++  +P   +GI  NL+ + LS VN+   V  D F  L+ SLE L+L D+ ++ +  NA + +P++      +N I+ I    F  + + L+ L +S  +  +  PN  F SLS+L+ L+L  N I+SL    F  +  L ++L L  N I       F +     L  ++L  N++E +E        L  L+L  N+I  L++++   +  L+ L + GN++  L                          TF+ L  L+ LD++ N++  +   AFDSI  L    IDLSHNQL + + +   N YP  +++ +LD+S+N ++++    F      L+ ++ S N+ S      +  L  ++ L+L HN + +I+D  F     L  L L HN + S+     +N  +LQ +DLSHN +  L  + F  T   +  ++HN L  +P    + P    L+ LDLS N+   I  D   +Q   L  L +  N IS I +DA   V N+++++L+ NPL    +         ++ LN+A T  T++P+L+   L SLN+S N ++ +  + L N   +  +D+S NE   L+   S+AW  + +L  L +S NP+  ++   F  L  L SL +S
Sbjct:   93 LKHLDFSHNQIRRLTERLFDGIEDSLEVLRLGNNLLGYNLNPVYSSSEFQNLGALRELDLSSNSLVDIDDGLFRGCKNLKDLKINGNKLKKVPTGALRGPKSLQNLFLQDNQISNGVKLGGSFITQPKLLSLNLTNNLIRVIRPTTFANVTRLVRLILTRNKLSSIVSDEFKELTGLVE-LDLSSNFLSTVPMQALVPLKTLRFLNLGSNLIKSIKEANFEALS-SLEYLDLSRNNIHEIIPGTFLGIG-NLKGLDLS-VNSLRKVEDDAFEGLT-SLEYLDLSDNKISQLPSNALERLPNLKRFKAHHNTIDSISSEDFIPIQD-LEELNLSYNMISQIFPN-TFASLSSLKILNLEYNFIKSLEGTAFQGLEDLLEYLNLAHNGIEILGPLNFSA-----LKYINLGSNYLEDIEGRFKNLRDLNVLILRYNKITNLNAKTLEGIDNLIKLDISGNELSDLEPGVLINPLLNEINLSSNALSEINTGTFKELPILEMLDISSNKISMIKKGAFDSIPRLK--TIDLSHNQLSSYKDDYFFNMYPNDTDLHTLDISHNELTYLYPESFR-FHQLLERVDFSNNLFSFFPTQFMKDLPNLKTLNLRHNLLKNIDDSEFANFLSLKVLNLEHNEVESVSETAFQNSSQLQFLDLSHNRISNLEGDTFKGTIRLLLDLSHNNLSSMPNGIFDRPKVVKLQSLDLSHNKFLRIPVDVLQSQYFFLDILKVSNNLISEIPSDANILV-NIKEIDLSFNPLSEDSVYNVLNEPKTVRSLNMAGTGXTVVPSLETPFLSSLNLSHNFISELNEDNL-NKPSLEVIDISHNEIPDLSYGLSSAWPLLTNLKYLDISSNPVKFIIKGDFKYLDGLRSLKMS 948          

HSP 2 Score: 85.1149 bits (209), Expect = 6.522e-17
Identity = 113/455 (24.84%), Postives = 201/455 (44.18%), Query Frame = 0
Query:   72 LRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLA----------------------------------------------------DNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNAL-RGLENSLEALDLSANRLQELPEDI------FMDFDTIRRLRLNDNMLQFSP---NITFNGFRYTIKDLNL----LGQDMYYVPLEEIGIMRNLRTVG-------------LSSVNNYGSVTTDQFADL--SPSLEALNLVDSDLTSIEH---NAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLP-LDTFHKMTRLQHL 441
            L E+NL+ NAL+ +  G F +L  LE LD+S N I  I   AF + +P L  +DL+                                                    +NL    P   +  + +L+ L+L +N ++ + D  F   L L  L L  N +++++  AFQN   + +  L+ N +  +E D F  T   LLDLS+  +  + +    R     L++LDLS N+   +P D+      F+D      L++++N++   P   NI  N     IK+++L    L +D  Y  L E   +R+L   G             LSS+N   +  ++   D    PSLE +++  +++  + +   +A+  + ++  +D+S+N +++I +  F+ + + L+ L+MSN      +    F+ L  L+ LDL +   + +P LDT   +   Q L
Sbjct:  553 LNEINLSSNALSEINTGTFKELPILEMLDISSNKISMIKKGAFDS-IPRLKTIDLSHNQLSSYKDDYFFNMYPNDTDLHTLDISHNELTYLYPESFRFHQLLERVDFSNNLFSFFPTQFMKDLPNLKTLNLRHNLLKNIDDSEFANFLSLKVLNLEHNEVESVSETAFQNSSQLQFLDLSHNRISNLEGDTFKGTIRLLLDLSHNNLSSMPNGIFDRPKVVKLQSLDLSHNKFLRIPVDVLQSQYFFLDI-----LKVSNNLISEIPSDANILVN-----IKEIDLSFNPLSEDSVYNVLNEPKTVRSLNMAGTGXTVVPSLETPFLSSLNLSHNFISELNEDNLNKPSLEVIDISHNEIPDLSYGLSSAWPLLTNLKYLDISSNPVKFIIKGDFKYL-DGLRSLKMSNLSLCNRIEGDAFKDLKELKELDLYS--YKKIPFLDTKGVLDHFQAL 993          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Match: EMLSAG00000009983 (supercontig:LSalAtl2s:LSalAtl2s652:91522:194127:1 gene:EMLSAG00000009983 transcript:EMLSAT00000009983 description:"maker-LSalAtl2s652-augustus-gene-2.26")

HSP 1 Score: 164.466 bits (415), Expect = 2.057e-41
Identity = 240/927 (25.89%), Postives = 396/927 (42.72%), Query Frame = 0
Query:  101 LSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFE-YINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEA---------------------------LDLSANRLQELPEDIFMDFDTIRRLRLNDNML-QFSPNITFNGFRYTIKDLN---LLGQDMYY---VPLEEIGIMRN-LRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRF-GKLQQLLLNDNRIKKLDSQSFV------------------------EMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIG---TLS------------------HLA-IDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRN------IISEITPD-DVGQLRRIRVLDLSHNCISHI---EDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLR-TSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQI-TTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTF-------GGIRDH---------LQGLNVANT---------SLTLLPNLQ--LSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
            + R+ I ++H   F +    L  L +++  +  +P  +I  V SL  L+L NNRI  V    F G   L  L L+ N + +L  N     E  I    LA N    I P + ++  +K L L N    +L++ +  G  N L++                           LD+S N+L  + ED F     +  L L+ N L +  P+I     R T  DL+   L G    +   + LEE+ +  N L ++G    NN            SP+L+ + L  + +  I+  + + +  +S + LS N +  I    FR    AL  L + N  H   +  G FR +  LR L L NN ++ +  + F  + +L  L+LQDN+I+  +   F   A   L  LDL  N + +VE    R+   L+ + L+ NRI     ++F                         E+  L  L+L  N+I  + D   Q LH+++ +DL+ NR+  L  A    +    TLS                  HL  +DLSHN+   +R+ R+  +     + +L L+   I F+     +  +  L +L +S+N       I  +TP+     L  ++VL LSHN +S+    +D T    + L ++ L    L+    + L    +L+ + L  N LE L EE+F +   LE   +  N+   +P +A   +  +L+ L+LS N +   + +S  + +  L  L+L GN I  I    F  +  L QLN+++N        TF         I +H         +QGL    T          L  +P  +  + S+ +  +S   L+ +    L     +  LDLS N+LT     ++  +  LL L LS N I+++ ++ FAGL  L +L++S
Sbjct:   71 IKRSGIKELHENIFMSTGNRLHTLVISECQLEAVPR-AITKVSSLMRLELDNNRISTVYPFTFFGPSKLSYLSLKSNRLDSLVENGLMGLENTIRELILADNHFS-IFPMSAIKI-LKKLTLLNLSQNKLSNVSFEGYNNRLDSLLNLEMSFNEFEILHMQTFSSMPNLKHLDVSHNQLHFIQEDTFFSQSNLAILDLSYNSLAELPPDIFHRCHRLTFVDLSHNRLKGLSQVFKDLIALEEVALNNNMLLSLGQDWFNN------------SPNLKTIRLQHNIIFEIQSESLQPLKRLSELHLSFNFLSVITRMDFR-YNVALNTLNLDNN-HISVIETGSFRGVPRLRELRLQNNVLKEVSRELFSSLAQLSELHLQDNKITKIEPQAFSGLA--LLQYLDLRGNKLTKVEDVMMRYPSTLRSMYLDGNRISSFHKETFSGQTTVEVLSISNNRLEIITPKMFEELINLFRLTLSNNRIVSIEDYALQGLHQVRVMDLSNNRIVKLTNATLKGLQQAETLSLNNNRINSISNEAFSGFHHLKELDLSHNR---IRMIRNGAFRGCLTLHTLRLNDAGIRFIAPNALDDFKK-LSVLEMSQNQLLIPEAIHRLTPNPGPSPLSSLKVLVLSHNDLSNATRSKDYTLSGLSNLETIELEGCNLSKFRKEFLWENTKLKTIRLGSNKLEKLEEEIFSKQVFLEELHLDRNKFKSVPREATRYL-FNLRLLNLSGNMLRENVDESSFDNVAGLKLLDLSGNSIKHIPERTFHNISGLLQLNISNNSFLGFQSRTFVPLVQLSSLISNHNTLPFIPIAIQGLRSVRTLCVGGNPLERLKEIPESRAVMHSVTTAQISGTNLSILETGDLGLFPNLINLDLSRNQLTMIAPYSFSKLTQLLRLDLSYNQILQLTSERFAGLESLSTLNLS 973          

HSP 2 Score: 138.658 bits (348), Expect = 1.736e-33
Identity = 217/856 (25.35%), Postives = 363/856 (42.41%), Query Frame = 0
Query:   71 SLRELNLAGNALTSVPEGIFWDLQRLERLDLSRN---NIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQL-HLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRN-LRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRF-GKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQ---LNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNEL-TSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRA 916
            SL  L L  N +++V    F+   +L  L L  N   ++++   M  +  +  L    LADN     P  +I  +K L +L+LS N++  V+   +  +L  L  L++  N  + L    F +   + +  ++ N L +I+ D F                          +++L  LDLS N L ELP DIF     +  + L+ N L+        G     KDL         + LEE+ +  N L ++G    NN            SP+L+ + L  + +  I+  + + +  +S + LS N +  I    FR    AL  L + N  H   +  G FR +  LR L L NN ++ +  + F  + +L  L+LQDN+I+  +   F   A   L  LDL  N + +VE    R+   L+ + L+ NRI     ++F   T + +LS+  N++  +  + F+ L  L RL L+ NR+ S+   A   +  +    +DLS+N++  L      T  TL  +   +                       L+L+ N I+ I+ +       ++ LDLSHN I  I +  F     L +L LN   +  +    L + K+L V+++S N L  +PE             A +RL   P    +P+ SSLK L LS N ++  + S+   L+ +  L  + LEG  +S    +       L+ + L SN L ++  + F                       +   L+ L++  N+   +P      L  +R L+LSGN L  +   +++ ++  L  L LSGN I  +   +F  +  L  L+IS      FQ R 
Sbjct:  113 SLMRLELDNNRISTVYPFTFFGPSKLSYLSLKSNRLDSLVENGLMGLENTIRELI---LADNHFSIFPMSAIKILKKLTLLNLSQNKLSNVSFEGYNNRLDSLLNLEMSFNEFEILHMQTFSSMPNLKHLDVSHNQLHFIQEDTFFS------------------------QSNLAILDLSYNSLAELPPDIFHRCHRLTFVDLSHNRLK--------GLSQVFKDL---------IALEEVALNNNMLLSLGQDWFNN------------SPNLKTIRLQHNIIFEIQSESLQPLKRLSELHLSFNFLSVITRMDFR-YNVALNTLNLDNN-HISVIETGSFRGVPRLRELRLQNNVLKEVSRELFSSLAQLSELHLQDNKITKIEPQAFSGLA--LLQYLDLRGNKLTKVEDVMMRYPSTLRSMYLDGNRISSFHKETFSGQTTVEVLSISNNRLEIITPKMFEELINLFRLTLSNNRIVSIEDYALQGLHQVR--VMDLSNNRIVKL------TNATLKGLQQAE----------------------TLSLNNNRINSISNEAFSGFHHLKELDLSHNRIRMIRNGAFRGCLTLHTLRLNDAGIRFIAPNALDDFKKLSVLEMSQNQL-LIPE-------------AIHRL--TPNPGPSPL-SSLKVLVLSHNDLSNATRSKDYTLSGLSNLETIELEGCNLSKFRKEFLWENTKLKTIRLGSNKLEKLEEEIFS----------------------KQVFLEELHLDRNKFKSVPREATRYLFNLRLLNLSGNMLRENVDESSFDNVAGLKLLDLSGNSIKHIPERTFHNISGLLQLNISNNSFLGFQSRT 839          

HSP 3 Score: 133.65 bits (335), Expect = 6.498e-32
Identity = 203/846 (24.00%), Postives = 359/846 (42.43%), Query Frame = 0
Query:   48 ERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE--TQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSN-------ALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLD---TFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKL-------------------------DSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQL-------QALRVNRS---------------NTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLP--------------------------EELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVY-----LNLEGNKIS---------------TIDADAFCCVPNLEQLNLA 787
            E  AL +N +  L  +D  + + +L+ + L  N +  +       L+RL  L LS N +  I  M F+  V  L  L+L +N +  I   S   V  LR L L NN +++V+   F     L  L L++N I  +   AF     + Y  L GN L  +E D  +   + ++ + L    I         G + ++E L +S NRL+ +   +F +   + RL L++N +    +    G  + ++ ++L    +  +    +  ++   T+ L++     S++ + F+     L+ L+L  + +  I + AF+   ++ ++ L++  I +I  NA  +    L  L MS        A+H R  PN     LS+L+ L LS+N + +       T   ++ L+ + L+   +S F++       N  L  + L  N +E++E + F +   L++L L+ N+ K +                         D  SF  +  L +L L GN I+ +P+ TF N+  L +L+++ N         F  +  LS L  +  HN L       Q LR  R+                +   + ++ +  +S  N+S +E        N L  L+LSRN ++ I P    +L ++  LDLS+N I  +    F     LS+L L++N L+SL     +  K L+++D+S N L++L                             L   TSL    ++ N   +IP+  LN VQ+SL+ LD   N      +S   +I L  +     ++  G+K+                TI     C +P++E L  +
Sbjct:  308 EEVALNNNMLLSLG-QDWFNNSPNLKTIRLQHNIIFEIQSESLQPLKRLSELHLSFNFLSVITRMDFRYNVA-LNTLNLDNNHISVIETGSFRGVPRLRELRLQNNVLKEVSRELFSSLAQLSELHLQDNKITKIEPQAFSGLALLQYLDLRGNKLTKVE-DVMMRYPSTLRSMYLDGNRISSFHKETFSG-QTTVEVLSISNNRLEIITPKMFEELINLFRLTLSNNRIVSIEDYALQGL-HQVRVMDLSNNRIVKLTNATLKGLQQAETLSLNNNRIN-SISNEAFSGFH-HLKELDLSHNRIRMIRNGAFRGCLTLHTLRLNDAGIRFIAPNALDDF-KKLSVLEMSQNQLLIPEAIH-RLTPNPGPSPLSSLKVLVLSHNDLSNATRSKDYTLSGLSNLETIELEGCNLSKFRKEFL--WENTKLKTIRLGSNKLEKLEEEIFSKQVFLEELHLDRNKFKSVPREATRYLFNLRLLNLSGNMLRENVDESSFDNVAGLKLLDLSGNSIKHIPERTFHNISGLLQLNISNNSFLGFQSRTFVPLVQLSSLISN--HNTLPFIPIAIQGLRSVRTLCVGGNPLERLKEIPESRAVMHSVTTAQISGTNLSILETGDLGLFPN-LINLDLSRNQLTMIAPYSFSKLTQLLRLDLSYNQILQLTSERFAGLESLSTLNLSYNLLSSLF-TFPKENKNLKILDVSFNRLQSLSIDSRSFIYLSSLLRLDLRGNRLSSIDSPLSPLTSLRALDLSDNLFTDIPLSDLNSVQNSLERLDFDGNPFICSCNSYTQKIWLXQHRKXLXVDRRGSKVGPSCFEPIHLKDRYLLTIKDSELCPLPSVESLGTS 1138          

HSP 4 Score: 130.568 bits (327), Expect = 7.044e-31
Identity = 151/561 (26.92%), Postives = 264/561 (47.06%), Query Frame = 0
Query:  357 AFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAH---NRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSL---KSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDI--SGVR 908
            A   V S+  ++L NNRI  +    F    + L YL + +      + NG     + +R L L++NH    P+     + +L  L L  N++S      ++++ + SL  L++SFN  E +   TF     L+ L ++ N++  +   +F   + L IL L  N + +LP + F   HRL  +DL++NRL+ L+   F  +  L  +A  L++N L +L  +  N  P                            +LK + L  NII EI  + +  L+R+  L LS N +S I    F     L++L L++N ++ +     R   RL+ + L +N L+ +  ELF  +SL      H   N++ +I  +A + + + L+YLDL  N++T + D  +     L  + L+GN+IS+   + F     +E L++++N L  I    F  + + L  L ++N  +  + +  L  L   + +++S N++  +   TL  L Q   L L+ N + S  + A+    HL  L LS N I  + N +F G   L +L +  +G+R
Sbjct:  107 AITKVSSLMRLELDNNRISTVYPFTFFGP-SKLSYLSLKSNRLDSLVENGLMGLENTIRELILADNHFSIFPMSAIKILKKLTLLNLSQNKLSNVSFEGYNNRLD-SLLNLEMSFNEFEILHMQTFSSMPNLKHLDVSHNQLHFIQEDTFFSQSNLAILDLSYNSLAELPPDIFHRCHRLTFVDLSHNRLKGLS-QVFKDLIALEEVA--LNNNMLLSLGQDWFNNSP----------------------------NLKTIRLQHNIIFEIQSESLQPLKRLSELHLSFNFLSVITRMDFRYNVALNTLNLDNNHISVIETGSFRGVPRLRELRLQNNVLKEVSRELF--SSLAQLSELHLQDNKITKIEPQAFSGL-ALLQYLDLRGNKLTKVEDVMMRYPSTLRSMYLDGNRISSFHKETFSGQTTVEVLSISNNRLEIITPKMFEELIN-LFRLTLSNNRIVSIEDYALQGLHQVRVMDLSNNRIVKLTNATLKGLQQAETLSLNNNRINSISNEAFSGFHHLKELDLSHNRIRMIRNGAFRGCLTLHTLRLNDAGIR 630          

HSP 5 Score: 115.931 bits (289), Expect = 2.197e-26
Identity = 215/919 (23.39%), Postives = 390/919 (42.44%), Query Frame = 0
Query:   51 ALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRN---LRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-------------------------FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRL---ESLN-FAAFDSIGTLSHLAI-DLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNIS------------------------------FVEVTFFEPVQND-----------------LKILNLSRNIISE-ITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHN---RLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNL-----QLSSLKSLNVSFNQLTF--IPPNTLANLSQVRELDLSGNELTS-----PPSTAWHSMD 873
            +L  N++  L    L  L  ++REL LA N  +  P      L++L  L+LS+N +  +    +   +  L  L+++ N    +   + + + +L+ LD+S+N++  + +  F  Q +L  L L  N++  L  + F     + +  L+ N L+ +         ++ + L+N ++  L  +      N L+ + L  N + E+  +       +  L L+ N L     IT   FRY +  LN L  D  ++ + E G  R    LR + L + N    V+ + F+ L+  L  L+L D+ +T IE  AF  +  +  +DL  N++  +E+   R   + L+ + + +     +     F   + +  L +SNN +  +    F ++  L  L L +N I + +   +  Q    + ++DLS N I ++   T +                         F  L++L L+ NRI+ + + +F     L  L L    IR +      +  +L  L+++ N+L   E+++          LS L +  LSHN L     ++  T   LSN+ +++L   N+S                              F +  F E +  D                 L++LNLS N++ E +       +  +++LDLS N I HI + TF   + L  L +++N       +      +L  +  +HN L  +P  +    S+    V  N   RL EIP      V  S+    +S   ++ +    L     L+ L+L  N+++ I   +F  +  L +L+L+ N + ++  + F G    L+ L+  N S  LL +L     +  +LK L+VSFN+L    I   +   LS +  LDL GN L+S      P T+  ++D
Sbjct:  142 SLKSNRLDSLVENGLMGLENTIRELILADNHFSIFPMSAIKILKKLTLLNLSQNKLSNVSFEGYNNRLDSLLNLEMSFNEFEILHMQTFSSMPNLKHLDVSHNQLHFIQEDTFFSQSNLAILDLSYNSLAELPPDIFHRCHRLTFVDLSHNRLKGLSQVFKDLIALEEVALNNNMLLSLGQDWFNNSPN-LKTIRLQHNIIFEIQSESLQPLKRLSELHLSFNFLSV---ITRMDFRYNVA-LNTLNLDNNHISVIETGSFRGVPRLRELRLQN-NVLKEVSRELFSSLA-QLSELHLQDNKITKIEPQAFSGLALLQYLDLRGNKLTKVEDVMMR-YPSTLRSMYL-DGNRISSFHKETFSGQTTVEVLSISNNRLEIITPKMFEELINLFRLTLSNNRIVSIE--DYALQGLHQVRVMDLSNNRIVKLTNATLKGLQQAETLSLNNNRINSISNEAFSGFHHLKELDLSHNRIRMIRNGAFRGCLTLHTLRLNDAGIRFIAPNALDDFKKLSVLEMSQNQLLIPEAIHRLTPNPGPSPLSSLKVLVLSHNDLSNATRSKDYTLSGLSNLETIELEGCNLSKFRKEFLWENTKLKTIRLGSNKLEKLEEEIFSKQVFLEELHLDRNKFKSVPREATRYLFNLRLLNLSGNMLRENVDESSFDNVAGLKLLDLSGNSIKHIPERTFHNISGLLQLNISNNSFLGFQSRTFVPLVQLSSLISNHNTLPFIPIAIQGLRSVRTLCVGGNPLERLKEIPES--RAVMHSVTTAQISGTNLSILETGDLGLFPNLINLDLSRNQLTMIAPYSFSKLTQLLRLDLSYNQILQLTSERFAG----LESLSTLNLSYNLLSSLFTFPKENKNLKILDVSFNRLQSLSIDSRSFIYLSSLLRLDLRGNRLSSIDSPLSPLTSLRALD 1043          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Match: EMLSAG00000007692 (supercontig:LSalAtl2s:LSalAtl2s447:512720:516392:1 gene:EMLSAG00000007692 transcript:EMLSAT00000007692 description:"maker-LSalAtl2s447-snap-gene-4.51")

HSP 1 Score: 161.384 bits (407), Expect = 1.085e-40
Identity = 192/733 (26.19%), Postives = 332/733 (45.29%), Query Frame = 0
Query:   46 RGERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALH--FRALPNGPFRSLSALRTLDLSNNHIRSLP-LDTFHKMTRLQHLYLQDNEIS-TFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTS------LEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKI 767
            R ++ +L  NQ+S L R+  +S    L  L+++ N  + +    F  L++L  L++S N I +I S  F      L VL L  N + ++   S+  ++ LR L LS+N I  V    F     + T+ L  NN+  +    F +  +I+  SL  N ++ I+ +AF    +  +++S+  +  L+S   +  EN +  LDLS N++  + ED F +        L  N L     +     +  +K LN+    +  +P      +  L  +   S NN   +    F+ L  +L  ++L  + LT ++   F  VP++  +DLS N++  +     R V   L  +R     H   + +P+ P     +L T+D S N+I+ +     +  M  L  L L  N++  + K G F       L  L L  N+I +  ++  R    L  L L+ N I+ L   +F  +  L  L L  N I  +  E F  L +LQ+LDL+ N +  +   AF  +  L    I++S N++Q L+      +    ++  ++LS+N ISF+    F                     P+      R++ +DLS+N I  +          L  L +++NRL  +   +L N   L+++D+S N   +L E++F   S      L +FK+ +N+   +PVKAL  +QS+LK LD+  N+I         +I+  + L  EGN +
Sbjct:  193 RLKKLSLFKNQLSSLPRDAFKSQG-QLEFLDISNNQFSKLEAHYFPKLRKLLWLNISHNEIPRIQSRVFARN-SLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDNFIPSVGRGAFRAVSRIGTVDLARNNLTKVDYQLFSDLRFIDTISLQENQIKNIDKEAFSNLYLTKINISHNQLPFLSSGLFKNCEN-MTFLDLSHNQIAHISEDAFNENTYATEFLLQYNKLTSMSGVPLAS-QKGLKFLNMSHNAIVDIPRNTFPKLYELFRLDF-SYNNISIIGKSIFSSLL-TLRHIDLSHNSLTKLDSGMFGKVPTLLDLDLSYNKLNQVR----RSVFGGLSSIRFVKLHHNQLQEIPSPPI----SLNTMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGNSIKAGRFDGLI--VLKSLGLRGNNITKPPWEALRSLISLNYLDLDYNLIQNLTKNAFGYLPVLFELGLSNNLIHNVSVEAFDGLLQLQKLDLSQNNITYIPPGAFKGMSAL--YDINISFNKIQHLQNRTHGLFEDTLSLRKIELSHNEISFITPKMF---------------------PESPWAPYRVKHIDLSYNLIHVLTKSILKGTKYLDYLNISNNRLNDVRKGILTNLTSLKILDISGN---SLKEDIFNNGSFGNMPNLTVFKLHNNKFETLPVKAL-LLQSNLKVLDIRNNKIKEYYTEFTPRIKKGMELYYEGNPL 882          

HSP 2 Score: 159.844 bits (403), Expect = 2.834e-40
Identity = 190/762 (24.93%), Postives = 354/762 (46.46%), Query Frame = 0
Query:  212 LETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDT-IRRLRLNDNML-QFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF---RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFD-------------SIGTLSHLAI---------DLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSL-------------------------KYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPN---LQLSSLKSLNVSFNQLTFIPPNT---LANLSQVRELDLSGNELT--SP---PSTAW--HSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVR 908
            ++T I  L +SNC I++L  +  + L  ++  L +    ++++ +  F    + +  L + +++L + SP+I       ++K L +    + Y+P   +  M +L  + +S      S+    F D+   L+ L+L  + L+S+  +AFK    +  +D+SNN+   +E + F ++   L +L +S+      + +  F   S LR L L+ N I  L  ++   M  L+ LYL DN I +  RG F  +A   +  +DL+ N++ +V+Y  F   RF  +  + L +N+IK +D ++F  +  L  +++  NQ+  L    F+N   +  LDL++N++  ++  AF+              + ++S + +         ++SHN +  +     NT+P L  +  LD SYNNIS +  + F                           L  +R +DLSHN ++ ++   FG    L  L L++N+L  +   +      ++ V L HN L+ +P       SL     ++N + EI  +   PV +SL                         K L L  N IT      L  +  L YL+L+ N I  +  +AF  +P L +L L++N +H + ++ F G+   LQ L+++  ++T +P      +S+L  +N+SFN++  +   T     +   +R+++LS NE++  +P   P + W  + + H   + LS N I  +      G ++L+ L+IS  R
Sbjct:   71 VKTLIHTLIISNCNIEKLYGSLFKLL--TIRILKIHDTPIKDISDGTFDQLSSSLEELHIQNSLLTRLSPSIKN---LTSLKILFIENSQISYLPEGVLNGMNSLEELSISH-GKIQSIGNKVFNDVR-RLKKLSLFKNQLSSLPRDAFKSQGQLEFLDISNNQFSKLEAHYFPKL-RKLLWLNISHN-EIPRIQSRVFARNSLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDNFIPSVGRGAF--RAVSRIGTVDLARNNLTKVDYQLFSDLRF--IDTISLQENQIKNIDKEAFSNLY-LTKINISHNQLPFLSSGLFKNCENMTFLDLSHNQIAHISEDAFNENTYATEFLLQYNKLTSMSGVPLASQKGLKFLNMSHNAIVDIP---RNTFPKLYELFRLDFSYNNISIIGKSIF-------------------------SSLLTLRHIDLSHNSLTKLDSGMFGKVPTLLDLDLSYNKLNQVRRSVFGGLSSIRFVKLHHNQLQEIPSP---PISLNTMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGNSIKAGRFDGLIVLKSLGLRGNNITKPPWEALRSLISLNYLDLDYNLIQNLTKNAFGYLPVLFELGLSNNLIHNVSVEAFDGLL-QLQKLDLSQNNITYIPPGAFKGMSALYDINISFNKIQHLQNRTHGLFEDTLSLRKIELSHNEISFITPKMFPESPWAPYRVKH---IDLSYNLIHVLTKSILKGTKYLDYLNISNNR 783          

HSP 3 Score: 155.606 bits (392), Expect = 6.416e-39
Identity = 200/809 (24.72%), Postives = 364/809 (44.99%), Query Frame = 0
Query:  138 SIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFL-ETQIKLLDLSNCLIQELASN---ALRGL-------------------ENSL-EALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYT--IKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLL---LNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLP---------DETFQNLHRLQ------------RLDLAYNRL-ESLNFAAFDSIGTLSHLAI---DLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFE--PVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSL----HGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIV 886
            SI ++ SL++L + N++I  + +    G   L+ L +    IQ++    F +   +   SL  N L  +  DAF  + Q++ LD+SN    +L ++    LR L                    NSL   L L+AN++  L  +       +RRL L+DN   F P++    FR    I  ++L   ++  V  +    +R + T+ L   N   ++  + F++L   L  +N+  + L  +    FK+  +++ +DLS+N+I +I E+AF E   A ++L   N L   ++   P  S   L+ L++S+N I  +P +TF K+  L  L    N IS   +  F S    +L  +DLS N + ++  D+  FGK+  LL   L+ N++ ++    F  ++ +  + L  NQ++++P         D ++ N+  ++             L+L YN+L  S+    FD +  L  L +   +++    +ALR        +L ++  LDL YN I  +    F   PV   L  L LS N+I  ++ +    L +++ LDLS N I++I    F   + L  + ++ N++  L    HG L  +   L+ ++LSHN +  +  ++F  +    ++V H                    +DLS N I  ++ S L   + L YLN+  N+++ +       + +L+ L+++ N L     D F             N S   +PNL +  L +     N+   +P   L   S ++ LD+  N++    +     +   + L+  GNP++
Sbjct:  139 SIKNLTSLKILFIENSQISYLPEGVLNGMNSLEELSISHGKIQSIGNKVFNDVRRLKKLSLFKNQLSSLPRDAFKSQGQLEFLDISNNQFSKLEAHYFPKLRKLLWLNISHNEIPRIQSRVFARNSLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDN---FIPSVGRGAFRAVSRIGTVDLARNNLTKVDYQLFSDLRFIDTISLQE-NQIKNIDKEAFSNLY--LTKINISHNQLPFLSSGLFKNCENMTFLDLSHNQIAHISEDAFNENTYATEFLLQYNKL--TSMSGVPLASQKGLKFLNMSHNAIVDIPRNTFPKLYELFRLDFSYNNISIIGKSIFSSLL--TLRHIDLSHNSLTKL--DSGMFGKVPTLLDLDLSYNKLNQVRRSVFGGLSSIRFVKLHHNQLQEIPSPPISLNTMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGNSIKAGRFDGLIVLKSLGLRGNNITKPPWEALR--------SLISLNYLDLDYNLIQNLTKNAFGYLPV---LFELGLSNNLIHNVSVEAFDGLLQLQKLDLSQNNITYIPPGAFKGMSALYDINISFNKIQHLQNRTHG-LFEDTLSLRKIELSHNEISFITPKMFPESPWAPYRVKH--------------------IDLSYNLIHVLTKSILKGTKYLDYLNISNNRLNDVRKGILTNLTSLKILDISGNSLKE---DIFN------------NGSFGNMPNLTVFKLHN-----NKFETLPVKALLLQSNLKVLDIRNNKIKEYYTEFTPRIKKGMELYYEGNPLM 883          

HSP 4 Score: 155.221 bits (391), Expect = 9.247e-39
Identity = 169/617 (27.39%), Postives = 275/617 (44.57%), Query Frame = 0
Query:  328 VTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQAL-RVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGI---------RDHLQGLNVANTSLTLL--------------PNLQLSSLKSLNVSFNQL-TFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRASD 918
            ++   F  LS SLE L++ +S LT +   + K++ S+  + + N++I Y+ E     + N+L+ L +S+    +++ N  F  +  L+ L L  N + SLP D F    +L+ L + +N+ S  +   F       L  L++S N I R++   F R   L+ L LN N+I +LD+ S   M  L  L L  N I  +    F+ + R+  +DLA N L  +++  F  +  +    I L  NQ++ + +   SN Y T  NI     S+N + F+    F+  +N                         +  LDLSHN I+HI +  F      +   L +N+LTS+ G  L +QK L+ +++SHN +  +P   F             +LYE            L  LD S N I+ I  S  + +  L +++L  N ++ +D+  F  VP L  L+L+ N L+++    FGG+          + LQ +     SL  +              P   ++SL  LN+ +NQL   I       L  ++ L L GN +T PP  A  S+  L  L L  N I  +  ++F  L  L  L +S     +  V A D
Sbjct:  111 ISDGTFDQLSSSLEELHIQNSLLTRLSP-SIKNLTSLKILFIENSQISYLPEGVLNGM-NSLEELSISHG-KIQSIGNKVFNDVRRLKKLSLFKNQLSSLPRDAFKSQGQLEFLDISNNQFSKLEAHYFPKLR--KLLWLNISHNEIPRIQSRVFARNSLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDNFIPSVGRGAFRAVSRIGTVDLARNNLTKVDYQLFSDLRFID--TISLQENQIKNIDKEAFSNLYLTKINI-----SHNQLPFLSSGLFKNCEN-------------------------MTFLDLSHNQIAHISEDAFNENTYATEFLLQYNKLTSMSGVPLASQKGLKFLNMSHNAIVDIPRNTF------------PKLYE------------LFRLDFSYNNISIIGKSIFSSLLTLRHIDLSHNSLTKLDSGMFGKVPTLLDLDLSYNKLNQVRRSVFGGLSSIRFVKLHHNQLQEIPSPPISLNTMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGNSIKAGRFDGLIVLKSLGLRGNNITKPPWEALRSLISLNYLDLDYNLIQNLTKNAFGYLPVLFELGLSNNLIHNVSVEAFD 666          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Match: EMLSAG00000010969 (supercontig:LSalAtl2s:LSalAtl2s736:33395:37479:1 gene:EMLSAG00000010969 transcript:EMLSAT00000010969 description:"maker-LSalAtl2s736-augustus-gene-0.4")

HSP 1 Score: 153.68 bits (387), Expect = 1.155e-38
Identity = 182/703 (25.89%), Postives = 324/703 (46.09%), Query Frame = 0
Query:  212 LETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFD-TIRRLRLNDNML-QFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVN--RSNTYPTLSNIMSLDLSYN---NISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLR-TSLEIFKVAHNRLYEIPVKALN--------------------PVQSSLKYLDLSANQIT-TISDSQLNQIQLLVYLNLEGNKIST--------------IDAD----------AFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNEL 861
            ++T I  L ++NC I++L  N  + L  ++  L +    ++++ +  F     ++  L + +++L + SP+I       ++K L +    + Y+P   +  + +L+ + +S   N  S+    F D+   L+ L+L  + L+S+  +AFK    +  +D+S+N    +E + F  +   L +L +S+      + +  F   S LR L L+ N I  L  ++   M  L+ LYL DN I +  RG F  +A   +  +DL+ N++ +V+Y                       Q F ++  +  +SL+ NQI+ +  E F NL+ L ++++++N++  L    F +   ++ L  DLSHNQ+  +  +    NTY T        L YN   ++S V +T+    Q  LK+LN+S N I +I  +   +L  +  LD SHN IS I    F     L  + L+HN LT L   +L     L  +DLS+N L  +   +F   +S+   K+ HN L E+P   ++                    PV +SL  L+L  NQ+  ++   + + + +L  + L GN IS               ID D          AF  +P L +L L++N ++ I ++ FGG                      L  L+ L++S N +T+IPP     +S + +++LS N++
Sbjct:   71 VKTLIHTLIINNCNIEKLYGNLFKPL--TIRILKIFDTPIKDISDGTFDQLSVSLEELHIKNSLLTRLSPSIKN---LTSLKILFIENSQISYLPEGVLNGLNSLKELSISH-GNIKSIGKKVFNDVR-QLKKLSLFKNQLSSLPRDAFKSQGQLEFLDISHNHFSKLEPHYFPNL-RKLLWLNISHN-EIPRIQSRVFARNSLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDNFIPSVGRGAF--RAVSRIGTVDLARNNLTKVDY-----------------------QLFSDLRFIDTISLQQNQIKNIDKEAFTNLY-LTKINISHNQIPFLGSGLFKNCENMTFL--DLSHNQIVHIAEDAFNENTYAT-----EFLLQYNKLTSMSGVPLTY----QKGLKVLNVSHNEIVDIPKNTFPKLYEVFRLDFSHNNISIIGKSVFSTLLSLRHIDLSHNSLTKLDSGMLGKVPTLLDIDLSYNKLNRVRRSVFGGLSSIRFVKLHHNELNEVPAPPISLNAMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGDSLEAGRFDGLIVLKSIGLRGNNISKPPWEALRSLRSVNYIDLDDNHIRNLSRNAFGRLPILFELGLSNNSINNISVEAFGG----------------------LLQLQKLDLSQNNITYIPPGAFKGMSALYDINLSFNKI 705          

HSP 2 Score: 150.599 bits (379), Expect = 1.198e-37
Identity = 157/610 (25.74%), Postives = 283/610 (46.39%), Query Frame = 0
Query:  328 VTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKV--AHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQ----LSSLKSLNVSFNQLT-FIPPNTLANLSQVRELDLSGNELTSPPSTAWHSM----------DH--------------LLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
            ++   F  LS SLE L++ +S LT +   + K++ S+  + + N++I Y                          LP G    L++L+ L +S+ +I+S+    F+ + +L+ L L  N++S+  R  F SQ    L  LD+S NH  ++E   F    KL  L ++ N I ++ S+ F   + L +L L  N+I +L   + + +  L+RL L+ N + S+   AF ++  +    +DL+ N L  +       +  L  I ++ L  N I  ++   F  +   L  +N+S N I  +          +  LDLSHN I HI +  F      +   L +N+LTS+ G  L  QK L+V+++SHN +  +P+  F +   E+F++  +HN +  I  K++     SL+++DLS N +T +    L ++  L+ ++L  NK++ +    F  + ++  + L  N L+ +           L  ++ +  ++  +   Q    ++SL  LN+ +NQL   +       L  ++ + L GN ++ PP  A  S+          +H              L  L LS N I  +  ++F GL  L+ LD+S
Sbjct:  111 ISDGTFDQLSVSLEELHIKNSLLTRLSP-SIKNLTSLKILFIENSQISY--------------------------LPEGVLNGLNSLKELSISHGNIKSIGKKVFNDVRQLKKLSLFKNQLSSLPRDAFKSQG--QLEFLDISHNHFSKLEPHYFPNLRKLLWLNISHNEIPRIQSRVFARNSLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDNFIPSVGRGAFRAVSRIG--TVDLARNNLTKVDYQ---LFSDLRFIDTISLQQNQIKNIDKEAFTNLY--LTKINISHNQIPFLGSGLFKNCENMTFLDLSHNQIVHIAEDAFNENTYATEFLLQYNKLTSMSGVPLTYQKGLKVLNVSHNEIVDIPKNTFPKL-YEVFRLDFSHNNI-SIIGKSVFSTLLSLRHIDLSHNSLTKLDSGMLGKVPTLLDIDLSYNKLNRVRRSVFGGLSSIRFVKLHHNELNEVPAPPIS-----LNAMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGDSLEAGRFDGLIVLKSIGLRGNNISKPPWEALRSLRSVNYIDLDDNHIRNLSRNAFGRLPILFELGLSNNSINNISVEAFGGLLQLQKLDLS 677          

HSP 3 Score: 132.494 bits (332), Expect = 6.320e-32
Identity = 165/671 (24.59%), Postives = 315/671 (46.94%), Query Frame = 0
Query:   70 LSLRELNLAGNALTSVPEGIFWDLQ-RLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE-TQIKLLDLSNCLIQELASNALRGLENS-LEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYT--IKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLL---LNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLP---------DETFQNLHRLQ------------RLDLAYNRL-ESLNFAAFDSIGTLSHLAI---DLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSL----HGKLLRNQKRLQVVDLSHNFLETLPEELF 703
            L++R L +    +  + +G F  L   LE L + +N+++   S + +  +  L +L + ++ +  +P   +  + SL+ L +S+  I+ +    F     L  L L +N + +L  +AF++   + +  ++ N    +EP  F    ++  L++S+  I  + S       NS L  L L+AN++  L  +       +RRL L+DN   F P++    FR    I  ++L   ++  V  +    +R + T+ L   N   ++  + F +L   L  +N+  + +  +    FK+  +++ +DLS+N+I +I E+AF E   A ++L   N L   ++   P      L+ L++S+N I  +P +TF K+  +  L    N IS   +  F +    SL  +DLS N + ++  D+   GK+  LL   L+ N++ ++    F  ++ +  + L  N++ ++P         D ++ N+  ++             L+L YN+L +SL    FD +  L  + +   ++S    +ALR  RS  Y  L +    +LS N    + + F          L LS N I+ I+ +  G L +++ LDLS N I++I    F   + L  + L+ N++  L    HG L  +   ++ +DLSHN +  +  ++F
Sbjct:   96 LTIRILKIFDTPIKDISDGTFDQLSVSLEELHI-KNSLLTRLSPSIKN-LTSLKILFIENSQISYLPEGVLNGLNSLKELSISHGNIKSIGKKVFNDVRQLKKLSLFKNQLSSLPRDAFKSQGQLEFLDISHNHFSKLEPHYFPNLRKLLWLNISHNEIPRIQSRVFA--RNSLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDN---FIPSVGRGAFRAVSRIGTVDLARNNLTKVDYQLFSDLRFIDTISLQQ-NQIKNIDKEAFTNL--YLTKINISHNQIPFLGSGLFKNCENMTFLDLSHNQIVHIAEDAFNENTYATEFLLQYNKL--TSMSGVPLTYQKGLKVLNVSHNEIVDIPKNTFPKLYEVFRLDFSHNNISIIGKSVFSTLL--SLRHIDLSHNSLTKL--DSGMLGKVPTLLDIDLSYNKLNRVRRSVFGGLSSIRFVKLHHNELNEVPAPPISLNAMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGDSLEAGRFDGLIVLKSIGLRGNNISKPPWEALRSLRSVNYIDLDDNHIRNLSRNAFGRLPILF---------ELGLSNNSINNISVEAFGGLLQLQKLDLSQNNITYIPPGAFKGMSALYDINLSFNKIKHLQNRTHG-LFEDTLSIRKIDLSHNQIPFITPKMF 740          

HSP 4 Score: 124.79 bits (312), Expect = 1.742e-29
Identity = 146/562 (25.98%), Postives = 255/562 (45.37%), Query Frame = 0
Query:   48 ERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLP-LDTFHKMTRLQHLYLQDNEIS-TFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFF 606
            ++ +L  NQ+S L R+  +S    L  L+++ N  + +    F +L++L  L++S N I +I S  F      L VL L  N + ++   S+  ++ LR L LS+N I  V    F     + T+ L  NN+  +    F +  +I+  SL  N ++ I+ +AF    +  +++S+  I  L S   +  EN +  LDLS N++  + ED F +        L  N L     +    ++  +K LN+   ++  +P      +  +  +  S  NN   +    F+ L  SL  ++L  + LT ++      VP++  +DLS N++  +     R V   L  +R    LH   L   P   +S L  +D S N+I+ +     +  M  L  L L  N++  + + G F       L  + L  N+I +  ++  R    +  + L+DN I+ L   +F  +  L  L L  N I  +  E F  L +LQ+LDL+ N +  +   AF  +  L    I+LS N+++ L+      +    +I  +DLS+N I F+    F
Sbjct:  195 KKLSLFKNQLSSLPRDAFKSQG-QLEFLDISHNHFSKLEPHYFPNLRKLLWLNISHNEIPRIQSRVFARN-SLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDNFIPSVGRGAFRAVSRIGTVDLARNNLTKVDYQLFSDLRFIDTISLQQNQIKNIDKEAFTNLYLTKINISHNQIPFLGSGLFKNCEN-MTFLDLSHNQIVHIAEDAFNENTYATEFLLQYNKLTSMSGVPLT-YQKGLKVLNVSHNEIVDIPKNTFPKLYEVFRLDFSH-NNISIIGKSVFSTLL-SLRHIDLSHNSLTKLDSGMLGKVPTLLDIDLSYNKLNRVR----RSVFGGLSSIRFV-KLHHNELNEVPAPPIS-LNAMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGDSLEAGRFDGLI--VLKSIGLRGNNISKPPWEALRSLRSVNYIDLDDNHIRNLSRNAFGRLPILFELGLSNNSINNISVEAFGGLLQLQKLDLSQNNITYIPPGAFKGMSAL--YDINLSFNKIKHLQNRTHGLFEDTLSIRKIDLSHNQIPFITPKMF 740          

HSP 5 Score: 68.1662 bits (165), Expect = 7.303e-12
Identity = 81/323 (25.08%), Postives = 145/323 (44.89%), Query Frame = 0
Query:   71 SLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQ----NFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRY--TIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIE---HNAFKHVPSISSMDLSNNRIEYIEENAFRE 384
             L+ LN++ N +  +P+  F  L  + RLD S NNI  I    F T +  L  +DL+ N + K+    +  V +L  +DLS N++ +V    F G   +  ++L  N +  + A        +  Y N   + G    W   ++ LE  +      N L   L +    GL   L+++ L  N + + P +      ++  + L+DN ++   N++ N F     + +L L    +  + +E  G +  L+ + LS  NN   +    F  +S +L  +NL  + +  ++   H  F+   SI  +DLS+N+I +I    F E
Sbjct:  432 GLKVLNVSHNEIVDIPKNTFPKLYEVFRLDFSHNNISIIGKSVFST-LLSLRHIDLSHNSLTKLDSGMLGKVPTLLDIDLSYNKLNRVRRSVFGGLSSIRFVKLHHNELNEVPAPPISLNAMDCSYNNIKEIKGRQ-PWPVMNSLLELNLDY----NQLGDSLEAGRFDGLI-VLKSIGLRGNNISKPPWEALRSLRSVNYIDLDDNHIR---NLSRNAFGRLPILFELGLSNNSINNISVEAFGGLLQLQKLDLSQ-NNITYIPPGAFKGMS-ALYDINLSFNKIKHLQNRTHGLFEDTLSIRKIDLSHNQIPFITPKMFPE 742          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Match: EMLSAG00000008016 (supercontig:LSalAtl2s:LSalAtl2s477:388792:422860:1 gene:EMLSAG00000008016 transcript:EMLSAT00000008016 description:"maker-LSalAtl2s477-augustus-gene-3.27")

HSP 1 Score: 149.828 bits (377), Expect = 6.561e-37
Identity = 182/666 (27.33%), Postives = 332/666 (49.85%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWD----LQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDT--------------------------LQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE-TQIKLLDLS-NCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRL-----------RLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGA-ANKLSSLYLNHNRLT 672
              DN IS +      S +  L E+NL GNA++    GI       +Q LE LD+S N + ++   +F + +P L  +DL+ N + ++   S  ++KSL++++LS NRIE+     F+   +++                           L+L EN I+++    F+++  +    L+GN +  I  +   +   +  LDLS N L  +  ++ +  LEN L+ L +S N+L+++ + I      +RRL            L++N LQ  P+ +       +K L L    +   P + I  ++NL+ +     N+   +T+  F  L+ SLE++ L D+ +  I+++ F+H+ S+  +DLS N I  +   A   + N ++ L +S+  +   LP   F SL  L TLD+SNN IR +  + F  M  L++L L  N +   ++GTF     PS+  L L  N I  +    F R  KL +L    N ++ + +++F ++  L  L +  N I+ L D +F++L+ L++L L  N + ++N  AF  I ++ +L  ++S N +Q L   RS  +  L  + ++DLS+N+I  ++   F+ ++   ++L L+ N + E+  +    +  +  LDL +N +S I   +F +  N++SS  +  N +T
Sbjct:  215 FSDNAISWIHPRAF-SYSHYLTEINLKGNAISDA--GIIGRAMGYVQSLEHLDISHNLLTEVKQGSF-SQIPQLRYIDLSYNSIYELGKRSFVNLKSLKIINLSRNRIERFMSDAFVDSSNIEKLFLNRNKIQSLSDINFLMDTLPRLRYLELSENEIRDIPFGVFRSYPKLERLFLSGNQISQISTEGLADMPSLSELDLSHNKLNVDQTASPIFNLEN-LKILKISDNKLRQINDKILKHLPLLRRLDITSQNKLEYISLSNNQLQNLPS-SLLSLDNRVKHLXLTSNFVRKFPDDLINKLKNLKKLLFIR-NSLDKLTSQSFLKLT-SLESIQLHDNRIEIIDNDTFQHLHSLKHLDLSLNIISTVGX-ALNPLSNLIK-LNLSDN-YITVLPQKTFTSLKRLSTLDVSNNTIREIS-EAFKDMPALKYLDLSYNHLPIIEKGTFSDL--PSILSLKLDHNSIVEIVPGAFDRLWKLDKLSFTKNGLQTIKTKTFTDLPSLESLYINDNLIKSLEDNSFEDLYSLKKLYLDQNLIVNINERAFKRIPSVRYL--NMSLNLIQELP--RSG-FLGLETLETIDLSFNDIRRIDDGTFDNMKWLTELL-LNDNNVCEVGKNVFNDVISLSSLDLQNNRLSRINIDSFSSLKNQISSFRIAGNPIT 860          

HSP 2 Score: 143.665 bits (361), Expect = 5.858e-35
Identity = 208/867 (23.99%), Postives = 349/867 (40.25%), Query Frame = 0
Query:   64 DLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINY------------------------TSLAGNPLEWIEPDAF-------------------------------LE--------------------TQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITF-----NGFRY------TIKDLNLLGQDMYYVPLEEIGIMRN----LRTVGLSSVN-------NYGSVTTDQFADLSP-----SLEALNLVDSDLTSIEHNAFKHVPSISSMDL-SNNRIEYIE---------ENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLR-TSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLT 817
            ++R    S+  L L  N + ++P   F  +  + RL +  N +  I   AF      +  L + +  +   P  S+ ++  L V  + N++I  +  P   G  +L   +L  +N++NL + + QN   + Y                         + + N + WI P AF                               LE                     Q++ +DLS   I EL   +   L+ SL+ ++LS NR++    D F+D   I +L LN N +Q   +I F        RY       I+D+   G    Y  LE + +  N    + T GL+ +        ++  +  DQ A  SP     +L+ L + D+ L  I     KH+P +  +D+ S N++EYI           ++   + N +++L +++    R  P+     L  L+ L    N +  L   +F K+T L+ + L DN I      TF  Q   SL  LDLS N I  V         L +L L+DN I  L  ++F  + RL  L +  N IR++  E F+++  L+ LDL+YN                 HL I                T+  L +I+SL L +N+                         I EI P    +L ++  L  + N +  I+  TF     L SLY+N N + SL      +   L+ + L  N +  + E  F R  S+    ++ N + E+P      ++ +L+ +DLS N I  I D   + ++ L  L L  N +  +  + F  V +L  L+L +N L RI++D+F  +++ +    +A   +T
Sbjct:   60 NIRKNVDSIDVLILEDNTILNLPSRAFGSIN-VNRLFIKNNRLHSIDRNAFAGIEDFITELYIKEPNLKNFPLESVDYLHKLEVFSIENSKIIDI--PRIAGLSNLKLFKLDGSNVRNLNSQSLQNLPTLKYLHVSRSNIRRIEVGLLESLHSLALANFSDNAISWIHPRAFSYSHYLTEINLKGNAISDAGIIGRAMGYVQSLEHLDISHNLLTEVKQGSFSQIPQLRYIDLSYNSIYELGKRSFVNLK-SLKIINLSRNRIERFMSDAFVDSSNIEKLFLNRNKIQSLSDINFLMDTLPRLRYLELSENEIRDIP-FGVFRSYPKLERLFLSGNQISQISTEGLADMPSLSELDLSHNKLNVDQTA--SPIFNLENLKILKISDNKLRQINDKILKHLPLLRRLDITSQNKLEYISLSNNQLQNLPSSLLSLDNRVKHLXLTSNF-VRKFPDDLINKLKNLKKLLFIRNSLDKLTSQSFLKLTSLESIQLHDNRIEIIDNDTF--QHLHSLKHLDLSLNIISTVGXALNPLSNLIKLNLSDNYITVLPQKTFTSLKRLSTLDVSNNTIREI-SEAFKDMPALKYLDLSYN-----------------HLPII------------EKGTFSDLPSILSLKLDHNS-------------------------IVEIVPGAFDRLWKLDKLSFTKNGLQTIKTKTFTDLPSLESLYINDNLIKSLEDNSFEDLYSLKKLYLDQNLIVNINERAFKRIPSVRYLNMSLNLIQELPRSGFLGLE-TLETIDLSFNDIRRIDDGTFDNMKWLTELLLNDNNVCEVGKNVFNDVISLSSLDLQNNRLSRINIDSFSSLKNQISSFRIAGNPIT 860          

HSP 3 Score: 135.576 bits (340), Expect = 1.678e-32
Identity = 199/828 (24.03%), Postives = 354/828 (42.75%), Query Frame = 0
Query:  141 HVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFE------YINYTSLAGNPLEWIEPDAFLE------------------TQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRY---------TIKDLNLLGQDMYYV-PLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFN--HIERVEYDTFRFGKLQQLLLNDNRIKKLDSQ-----------SFVEMTRLLILSLEGNQ------------------------IRQLPD------------------------ETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLR-TSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNL--QLSSLKSLNVSFNQLTFIPPNTLANLS-QVRELDLSGNELTSPPSTAW 869
            +V S+ VL L +N I  +    F G ++++ L ++ N + ++  NAF   E      YI   +L   PLE ++    LE                  + +KL  L    ++ L S +L+ L  +L+ L +S + ++ +   +     ++     +DN + +     F+   Y          I D  ++G+ M YV  LE + I  NL T           V    F+ + P L  ++L  + +  +   +F ++ S+  ++LS NRIE    +AF +  N  +     N +   +  N    +L  LR L+LS N IR +P   F    +L+ L+L  N+IS            PSL+ LDLS N  ++++     F    L+ L ++DN++++++ +                 +L  +SL  NQ                        +R+ PD                        ++F  L  L+ + L  NR+E ++   F  + +L HL  DLS N +  +          LSN++ L+LS N I+ +    F  ++  L  L++S N I EI+ +    +  ++ LDLS+N +  IE  TF     + SL L+HN +  +         +L  +  + N L+T+  + F    SLE   +  N +  +   +   +  SLK L L  N I  I++    +I  + YLN+  N I  +    F  +  LE ++L+ N + RI   TF  ++   + L   N    +  N+   + SL SL++  N+L+ I  ++ ++L  Q+    ++GN +T    T W
Sbjct:   64 NVDSIDVLILEDNTILNLPSRAF-GSINVNRLFIKNNRLHSIDRNAFAGIEDFITELYIKEPNLKNFPLESVDYLHKLEVFSIENSKIIDIPRIAGLSNLKLFKLDGSNVRNLNSQSLQNLP-TLKYLHVSRSNIRRIEVGLLESLHSLALANFSDNAISWIHPRAFSYSHYLTEINLKGNAISDAGIIGRAMGYVQSLEHLDISHNLLT----------EVKQGSFSQI-PQLRYIDLSYNSIYELGKRSFVNLKSLKIINLSRNRIERFMSDAFVDSSNIEKLFLNRNKIQSLSDINFLMDTLPRLRYLELSENEIRDIPFGVFRSYPKLERLFLSGNQISQISTEGLADM--PSLSELDLSHNKLNVDQTASPIFNLENLKILKISDNKLRQINDKILKHLPLLRRLDITSQNKLEYISLSNNQLQNLPSSLLSLDNRVKHLXLTSNFVRKFPDDLINKLKNLKKLLFIRNSLDKLTSQSFLKLTSLESIQLHDNRIEIIDNDTFQHLHSLKHL--DLSLNIISTV----GXALNPLSNLIKLNLSDNYITVLPQKTFTSLKR-LSTLDVSNNTIREIS-EAFKDMPALKYLDLSYNHLPIIEKGTFSDLPSILSLKLDHNSIVEIVPGAFDRLWKLDKLSFTKNGLQTIKTKTFTDLPSLESLYINDNLIKSLEDNSFEDLY-SLKKLYLDQNLIVNINERAFKRIPSVRYLNMSLNLIQELPRSGFLGLETLETIDLSFNDIRRIDDGTFDNMKWLTELLLNDNNVCEVGKNVFNDVISLSSLDLQNNRLSRINIDSFSSLKNQISSFRIAGNPITCDCDTLW 867          

HSP 4 Score: 57.7658 bits (138), Expect = 1.434e-8
Identity = 65/273 (23.81%), Postives = 129/273 (47.25%), Query Frame = 0
Query:  637 IRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRN-QKRLQVVDLSHNFLETLP-EELFLRTSLEIFKVAHNRLYEIP-VKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGG----IRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESL 902
            I VL L  N I ++    FG+ N ++ L++ +NRL S+        +  +  + +    L+  P E +     LE+F + ++++ +IP +  L    S+LK   L  + +  ++   L  +  L YL++  + I  I+      + +L   N + N +  IH   F         +L+G  +++  +       + SL+ L++S N LT +   + + + Q+R +DLS N +      ++ ++  L  ++LS N I R M+D+F    ++E L
Sbjct:   68 IDVLILEDNTILNLPSRAFGSIN-VNRLFIKNNRLHSIDRNAFAGIEDFITELYIKEPNLKNFPLESVDYLHKLEVFSIENSKIIDIPRIAGL----SNLKLFKLDGSNVRNLNSQSLQNLPTLKYLHVSRSNIRRIEVGLLESLHSLALANFSDNAISWIHPRAFSYSHYLTEINLKGNAISDAGIIGRAMGYVQSLEHLDISHNLLTEVKQGSFSQIPQLRYIDLSYNSIYELGKRSFVNLKSLKIINLSRNRIERFMSDAFVDSSNIEKL 335          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Match: EMLSAG00000008764 (supercontig:LSalAtl2s:LSalAtl2s543:55755:60198:-1 gene:EMLSAG00000008764 transcript:EMLSAT00000008764 description:"maker-LSalAtl2s543-augustus-gene-0.22")

HSP 1 Score: 141.354 bits (355), Expect = 2.263e-34
Identity = 170/579 (29.36%), Postives = 280/579 (48.36%), Query Frame = 0
Query:  225 LIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMS-NALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEIST--FKRGTFHSQANPSLAILDLSFNHIERVEY-DTFRF-GKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDT 798
            L+ E     L G E SLE LDLS N + + PE +     +I+             N +FN                    LE++G +RN               ++ ++     SLE+L L  + + SI   A   +PS+ S+D+SNN +  +  +AF+ +GN L++L +S NAL   ALP   F+ LS L +L + NN + ++ ++ F  +  L+ L L  N +     K G F      SL +LDLSFNH+ ++E  + F +   L+ L L+ NR+K + SQ F     L+IL+L  N++  +  +T + L  L+ L L +N++ SL    F++   L  L   L++N L  +     N +  L  ++ LDLS N I  ++   F+ +  +LK L ++ N +S +         ++  LDLS N    +E  TF   N L SL +  N L  ++G +L +  +L+ +++S N L    +  FL  +LE   V +NR+  +          SLKYLD S N+I  +    L+    L +L L  N I  I+A+ F    NL +++L  N L ++ +++
Sbjct:  122 LVMEPXYEXLXGXE-SLETLDLSFNNVLKFPEGLLCPLKSIQLF-----------NASFNNL----------------TNLEDLG-LRN---------------SSGKYCQNISSLESLILKGNSIQSISPGAISALPSLLSLDISNNGLRVLVNDAFQGLGN-LKFLDLSHNAL--VALPPDIFKGLSKLESLSIGNNSLGTIDINVFSNLGSLKSLNLSGNSLDENWLKPGIFDHLI--SLLLLDLSFNHLSKLEQPELFAYIENLEALNLDSNRLKSISSQIFRLTPNLVILTLSRNELETIQYKTLEGLTHLESLFLDHNKIHSLQSGTFENCTRLQKLK--LNNNYLTQV----PNAFHDLEALIYLDLSSNVIGVIKRESFKGL-GELKTLRIANNGLSRVGEGAFKDTSQLMHLDLSINRFKALERETFTVLNNLQSLNMAENLLEDING-VLSSLVQLKWLNISSNHLGWF-DYAFLPLNLEWLXVBNNRVXSLANYFGIKGDYSLKYLDASFNKIRIL--KPLSIPSSLTHLYLNDNNIEEIEANTFLDKRNLSRVHLERNNLSKLEMNS 640          

HSP 2 Score: 121.709 bits (304), Expect = 2.814e-28
Identity = 217/832 (26.08%), Postives = 346/832 (41.59%), Query Frame = 0
Query:  117 GVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKL-LDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFR--YTIKDLNLLGQDMYYVPLEEIGIMRN--LRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSH----------NQLQALRVNRSNTYPTLSNIMSLD----LSYNNISFVEVTFFEP--VQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIE--------------DPTF-----------------GAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIF-KVAHNRLYEIPVKALNP-VQSSLKYLDLSANQ-ITTISDSQLNQIQLLV-----YLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQL----SSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGN 883
            G   L  LDL+ N + K P   +  +KS+++ + S N +  + D           L LR     N +    QN   +    L GN ++ I P A       L LD+SN  ++ L ++A +GL N L+ LDLS N L  LP DIF     +  L + +N L     I  N F    ++K LNL G  +    L+  GI  +     +   S N+   +   +      +LEALNL  + L SI    F+  P++  + LS N +E I            QY  +    H              L +L L +N I SL   TF   TRLQ L L +N ++      FH     +L  LDLS N I  ++ ++F+  G+L+ L + +N + ++   +F + ++L+ L L  N+ + L  ETF  L+ LQ L++A N LE +N     S+  L  L I  +H            L+ L V+ +N   +L+N   +     L Y + SF ++   +P  + + L  L L+ N I EI  +     R +  + L  N +S +E               PTF                 G   +L   Y +   + +L     RN     ++ LS    +  PEE   R     F        +    +   P  +    + D + N  +   S   LN I   +      L+L+GN  + + +  F     L +L L ++ +  +   +F G+   L  L+++   L  L   +     S+L+ L +  NQL  +  N   NL ++R L L GN L S P  +      L+SL L+ N
Sbjct:  133 GXESLETLDLSFNNVLKFPEGLLCPLKSIQLFNASFNNLTNLED-----------LGLR-----NSSGKYCQNISSLESLILKGNSIQSISPGAISALPSLLSLDISNNGLRVLVNDAFQGLGN-LKFLDLSHNALVALPPDIFKGLSKLESLSIGNNSLG---TIDINVFSNLGSLKSLNLSGNSLDENWLKP-GIFDHLISLLLLDLSFNHLSKLEQPELFAYIENLEALNLDSNRLKSISSQIFRLTPNLVILTLSRNELETI------------QYKTLEGLTH--------------LESLFLDHNKIHSLQSGTFENCTRLQKLKLNNNYLTQVPNA-FHDLE--ALIYLDLSSNVIGVIKRESFKGLGELKTLRIANNGLSRVGEGAFKDTSQLMHLDLSINRFKALERETFTVLNNLQSLNMAENLLEDIN-GVLSSLVQLKWLNISSNHLGWFDYAFLPLNLEWLXVB-NNRVXSLANYFGIKGDYSLKYLDASFNKIRILKPLSIPSSLTHLYLNDNNIEEIEANTFLDKRNLSRVHLERNNLSKLEMNSLVLSSSDTIIDSPTFYFEDNPFICDCHLQWFQGINTRLHH-YPHVMDMDTLTCSSRRNSVYAVLIPLS----DVPPEEFLCRYESHCFPSCMCCDFFACDCRLKCPEEEGCFCFRDSTWNSNVVKCSTRSLNSIPSTIPVDATTLHLDGNNFTEVSSAMFIGRVKLTKLFLNNSRVTTMSNGSFAGL-PRLTILDLSQNRLQELNGPEFAGLESTLRELYLQGNQLVRMKANAFRNLGELRVLRLDGNLLISFPVWSLAHNPSLISLSLAHN 906          

HSP 3 Score: 102.064 bits (253), Expect = 4.036e-22
Identity = 185/819 (22.59%), Postives = 318/819 (38.83%), Query Frame = 0
Query:   71 SLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQ-------------------------------TGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLE--WIEPDAFLETQIKLLDLSNC-LIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNL-RTVGLSSVNNYGSVTTDQFADLSP-----------------------SLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRS-----LPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQL----------------PDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYP----------TLSNIMSLDL-----------SYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASN 789
            SL  L+L+ N +   PEG+   L+ ++  + S NN+  +  +  +                               + +P L  LD+++N +  +   +   + +L+ LDLS+N +  +    F G   L++L +  N++  +  N F N   +   +L+GN L+  W++P  F      LL   +   + +L    L     +LEAL+L +NRL+ +   IF     +  L L+ N L+     T  G  +    L  L  D   +   + G   N  R   L   NNY +   + F DL                          L+ L + ++ L+ +   AFK    +  +DLS NR + +E   F  V N LQ L M+  L      NG   SL  L+ L++S+NH+       LPL+       L+ L + +N + +     F  + + SL  LD SFN I R+         L  L LNDN I+++++ +F++   L  + LE N + +L                P   F++   +    L + +  +     +  +  +  L      N + A+ +  S+  P             + M  D                  F + T+     N +K    S N I    P D         L L  N  + +    F    KL+ L+LN++R+T++         RL ++DLS                        NRL E+       ++S+L+ L L  NQ+  +  +    +  L  L L+GN + +    +    P+L  L+LA N
Sbjct:  136 SLETLDLSFNNVLKFPEGLLCPLKSIQLFNASFNNLTNLEDLGLRNSSGKYCQNISSLESLILKGNSIQSISPGAISALPSLLSLDISNNGLRVLVNDAFQGLGNLKFLDLSHNALVALPPDIFKGLSKLESLSIGNNSLGTIDINVFSNLGSLKSLNLSGNSLDENWLKPGIFDHLISLLLLDLSFNHLSKLEQPELFAYIENLEALNLDSNRLKSISSQIFRLTPNLVILTLSRNELETIQYKTLEGLTH----LESLFLDHNKIHSLQSGTFENCTRLQKLKLNNNYLTQVPNAFHDLEALIYLDLSSNVIGVIKRESFKGLGELKTLRIANNGLSRVGEGAFKDTSQLMHLDLSINRFKALERETF-TVLNNLQSLNMAENLLEDI--NGVLSSLVQLKWLNISSNHLGWFDYAFLPLN-------LEWLXVBNNRVXSLA-NYFGIKGDYSLKYLDASFNKI-RILKPLSIPSSLTHLYLNDNNIEEIEANTFLDKRNLSRVHLERNNLSKLEMNSLVLSSSDTIIDSPTFYFEDNPFICDCHLQWFQGINTRLHHYPHVMDMDTLTCSSRRNSVYAVLIPLSDVPPEEFLCRYESHCFPSCMCCDFFACDCRLKCPEEEGCFCFRDSTW---NSNVVKCSTRSLNSIPSTIPVDA------TTLHLDGNNFTEVSSAMFIGRVKLTKLFLNNSRVTTMSNGSFAGLPRLTILDLS-----------------------QNRLQELNGPEFAGLESTLRELYLQGNQLVRMKANAFRNLGELRVLRLDGNLLISFPVWSLAHNPSLISLSLAHN 906          

HSP 4 Score: 96.2857 bits (238), Expect = 2.217e-20
Identity = 159/625 (25.44%), Postives = 261/625 (41.76%), Query Frame = 0
Query:  394 MSNALHFRA-LPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQ--DNEISTFKRGTFHSQ--ANPSLAILDLSFNHI-----------ERVEYDTFRF---------------GK-------LQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLS--------------------------YNNISFVE-VTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNL--QLSSLKSLNVSFNQL-----TFIPPN------------TLANLSQVRE------LDLSGNELT-----SPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLR-----HLESLDISGVRAKSFQVRASD 918
             S+ L+F + L +  F  LS L TL + N  + +LP  +F  + RL+ L +Q  + ++S       +       SL  LDLSFN++           + ++     F               GK       L+ L+L  N I+ +   +   +  LL L +  N +R L ++ FQ L  L+ LDL++N L +L    F  +  L  L+I   +N L  + +N    +  L ++ SL+LS                          +N++S +E    F  ++N L+ LNL  N +  I+         + +L LS N +  I+  T      L SL+L+HN++ SL      N  RLQ + L++N+L  +P                N  +++          +L YLDLS+N I  I       +  L  L +  N +S +   AF     L  L+L+ N    +  +TF  + ++LQ LN+A   L  +  +   L  LK LN+S N L      F+P N            +LAN   ++       LD S N++      S PS+  H       L+L+ N I  +  ++F   R     HLE  ++S +   S  + +SD
Sbjct:   62 CSDVLYFESQLRSDHFGELSRLSTLRIHNCKVHTLPPRSFVSLIRLRELVIQTHNADMSGLVMEPXYEXLXGXESLETLDLSFNNVLKFPEGLLCPLKSIQLFNASFNNLTNLEDLGLRNSSGKYCQNISSLESLILKGNSIQSISPGAISALPSLLSLDISNNGLRVLVNDAFQGLGNLKFLDLSHNALVALPPDIFKGLSKLESLSI--GNNSLGTIDIN---VFSNLGSLKSLNLSGNSLDENWLKPGIFDHLISLLLLDLSFNHLSKLEQPELFAYIEN-LEALNLDSNRLKSISSQIFRLTPNLVILTLSRNELETIQYKTLEGLTHLESLFLDHNKIHSLQSGTFENCTRLQKLKLNNNYLTQVP----------------NAFHDL---------EALIYLDLSSNVIGVIKRESFKGLGELKTLRIANNGLSRVGEGAFKDTSQLMHLDLSINRFKALERETF-TVLNNLQSLNMAENLLEDINGVLSSLVQLKWLNISSNHLGWFDYAFLPLNLEWLXVBNNRVXSLANYFGIKGDYSLKYLDASFNKIRILKPLSIPSSLTH-------LYLNDNNIEEIEANTFLDKRNLSRVHLERNNLSKLEMNSLVLSSSD 647          

HSP 5 Score: 68.1662 bits (165), Expect = 9.079e-12
Identity = 158/623 (25.36%), Postives = 249/623 (39.97%), Query Frame = 0
Query:   48 ERCALGDNQISELSREDLRSLALSLRELNLAGNALTS--VPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAF--LETQIKLLDLSNCLIQELASNALRGL-----------------------ENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDL-------NLLGQ-DMYYVPLE-EIGIMRNLRTVGLSSVNNYGSVTTD---QFADLS-------------PSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLP----LDTFHKMTRLQHLY-----LQDNEISTFKRGTFHSQANPSLAILDLSFNHIERV----EYDTFRFG-------------------------KLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLN---FAAFDSIGTLSHLAIDLSHNQLQALRVN 577
            E  ++G+N +  +      +L  SL+ LNL+GN+L    +  GIF  L  L  LDLS N++ ++        + +L  L+L  N +  I         +L +L LS N +E +      G  HL++L L  N I +L +  F+N   +    L  N L  + P+AF  LE  I  LDLS+ +I  +   + +GL                        + L  LDLS NR + L  + F   + ++ L + +N+L+       NG   ++  L       N LG  D  ++PL  E   + N R   L+   NY  +  D   ++ D S              SL  L L D+++  IE N F    ++S + L  N +  +E N+   V ++   +  S   +F    + PF     L+     N  +   P    +DT    +R   +Y     L D     F    + S   PS    D  F    R+    E   F F                              L L+ N   ++ S  F+   +L  L L  +++  + + +F  L RL  LDL+ NRL+ LN   FA  +S  TL  L   L  NQL  ++ N
Sbjct:  266 ESLSIGNNSLGTIDINVFSNLG-SLKSLNLSGNSLDENWLKPGIFDHLISLLLLDLSFNHLSKLEQPELFAYIENLEALNLDSNRLKSISSQIFRLTPNLVILTLSRNELETIQYKTLEGLTHLESLFLDHNKIHSLQSGTFENCTRLQKLKLNNNYLTQV-PNAFHDLEALI-YLDLSSNVIGVIKRESFKGLGELKTLRIANNGLSRVGEGAFKDTSQLMHLDLSINRFKALERETFTVLNNLQSLNMAENLLE-----DINGVLSSLVQLKWLNISSNHLGWFDYAFLPLNLEWLXVBNNRVXSLA---NYFGIKGDYSLKYLDASFNKIRILKPLSIPSSLTHLYLNDNNIEEIEANTFLDKRNLSRVHLERNNLSKLEMNSL--VLSSSDTIIDSPTFYFE---DNPFICDCHLQWFQGINTRLHHYPHVMDMDTLTCSSRRNSVYAVLIPLSDVPPEEF-LCRYESHCFPSCMCCDF-FACDCRLKCPEEEGCFCFRDSTWNSNVVKCSTRSLNSIPSTIPVDATTLHLDGNNFTEVSSAMFIGRVKLTKLFLNNSRVTTMSNGSFAGLPRLTILDLSQNRLQELNGPEFAGLES--TLRELY--LQGNQLVRMKAN 866          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Match: EMLSAG00000012107 (supercontig:LSalAtl2s:LSalAtl2s869:139642:146365:-1 gene:EMLSAG00000012107 transcript:EMLSAT00000012107 description:"maker-LSalAtl2s869-augustus-gene-1.6")

HSP 1 Score: 137.502 bits (345), Expect = 3.631e-33
Identity = 154/581 (26.51%), Postives = 267/581 (45.96%), Query Frame = 0
Query:  302 YVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGK--------LQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNIS--FVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLR--TSLEIFKVAHNRLYEIP--VKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNL--QLSSLKSLNVSFNQL-----TFIPPNTLANLSQVRELDLSGNEL 861
            + P +EI +  +++++  +  N+  + +  + + L  +   +  ++S+L S     F H+P++  + +    ++ I   +F+ +G  L  L +             F  L  LR+L+LS+N++ S+P  TF +++                           L+ L+LSFN ++ +   +F   K        L+ L+L+DN   ++ SQ+   +++L  L L  N I  + D  F NL  L+ L LA N+L +L    F     L  L++     Q  +L V     +  LS ++ L++S N I   ++    F  +   L  L+LS N IS + P  +  L  +++LDLSHN ++ ++  TF + N L  L LNHN L SLH +       +  ++L  N L  L E  FL   ++L    +++NR   +P  ++ L+     LK LDL  N I  I +     ++ L  L L  N I+ I    F  +P ++ LNLA N +  +    F  +R+ L+ L +    L  +  L      L+ LNVS N+L      FIP N       +  LD+ GN++
Sbjct:   48 FEPKKEI-LKCSIQSLDFAVGNSTNTASYSKASVLEFTCSQIYFLESNLRS---GHFGHLPTLKELRIQGCPMKKIPSGSFQGLG-GLTSLSVVTQDSLELDSERAFEGLDHLRSLNLSHNNLWSVPPGTFCRLS--------------------------GLSTLNLSFNRLQDMHELSFSPSKRSTSCKLPLENLILSDNSYVEIPSQALSHVSKLETLELSSNAINLVQDNAFDNLRDLKYLSLANNQLIALPPHTFAPTPHLRELSL-----QNNSLGVLAPKIFSGLSRLLVLNVSRNEIGNEWLTPALFASLVR-LVSLDLSHNRISSLDPSVLRPLTSLQILDLSHNLLTTLDSGTFLSQNNLHILRLNHNDLKSLHARSFDGLSVVSSINLDSNALSALEENAFLDCCSTLNDLTLSNNRFSRVPDGLQGLD----GLKTLDLGGNNIKEIHNGSFTGLRALYGLKLSSNGITNISESIFKPIPGVQVLNLAFNKIKSLGQGVFNKLRN-LKMLRLDGNELIDINGLLSGQEELQWLNVSGNRLQWFDYAFIPKN-------LELLDIHGNQI 579          

HSP 2 Score: 135.576 bits (340), Expect = 1.507e-32
Identity = 211/866 (24.36%), Postives = 361/866 (41.69%), Query Frame = 0
Query:   72 LRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQ-------TGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE-TQIKLLDLS-NCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSN---IMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSS----------LYLNHNRL-TSLHGKLLRN-----QKRLQVV----------DLSHNFLETLPEELFLRTSLEIFKVAH-NRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLN-----LEGNKIST-IDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQ---LSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWH-----SMDHLLSLHLSGNP 884
            LR LNL+ N L SVP G F  L  L  L+LS N +  +H ++F          +P L  L L+DN   +IP  +++HV  L  L+LS+N I  V D  F     L  L L  N +  L  + F    ++   SL  N L  + P  F   +++ +L++S N +  E  + AL      L +LDLS NR+  L   +     +++ L L+ N+L             T+     L Q+                                       +L  L L  +DL S+   +F  +  +SS++L +N +  +EENAF +  + L  L +SN   F  +P+G  + L  L+TLDL  N+I+ +   +F  +  L  L L  N I+      F  +  P + +L+L+FN                       +IK L    F ++  L +L L+GN++  + +        LQ L+++ NRL+  ++A       L    +D+  NQ++ L       Y  L++   + + D S+N I  +E   F      +++ NL+ N I  +TP       ++R +DLSHN +  ++  +F  A    S           Y+ HN L      +  +N     Q RL ++          D S + L+   E    +     F +      +    +   P      +    +  +   S      +  L+ ++     ++GN  +  +D  +F     +  + L ++ +  I   TF G+ + L+ L++ +  +  L   +   +SSL+ L +  N++ +I   T  NL  +R L L GN L S  ++ W      S+  L S+ LS NP
Sbjct:  155 LRSLNLSHNNLWSVPPGTFCRLSGLSTLNLSFNRLQDMHELSFSPSKRSTSCKLP-LENLILSDNSYVEIPSQALSHVSKLETLELSSNAINLVQDNAFDNLRDLKYLSLANNQLIALPPHTFAPTPHLRELSLQNNSLGVLAPKIFSGLSRLLVLNVSRNEIGNEWLTPALFASLVRLVSLDLSHNRISSLDPSVLRPLTSLQILDLSHNLLT------------TLDSGTFLSQN---------------------------------------NLHILRLNHNDLKSLHARSFDGLSVVSSINLDSNALSALEENAFLDCCSTLNDLTLSNN-RFSRVPDG-LQGLDGLKTLDLGGNNIKEIHNGSFTGLRALYGLKLSSNGITNISESIF--KPIPGVQVLNLAFN-----------------------KIKSLGQGVFNKLRNLKMLRLDGNELIDI-NGLLSGQEELQWLNVSGNRLQWFDYAFIPKNLEL----LDIHGNQIENL-----GNYXKLTDQFELKTXDXSHNKIKKLETLSFP---KSIQVANLNHNRIRLLTPGMFRDKIKLRKVDLSHNYLKRLQISSFNIAPYSESTSIQEEPRPHFYMGHNPLHCDCEMEWFKNMNEMTQARLPIIMDYSSIQCHGDSSTSILDIPNEHFLCQYEAHCFSLCMCCDFFACDCRMQCPEGCDCYHDSSWSVNVIQCSXRHHALVPPLIPMDATNIYMDGNNFTGLLDTQSFIGRKRISSMFLNNSNIGGIDNQTFNGLME-LKILHLEDNQVQRLEGYEFVNISSLQELYLHRNRIEYIHERTFDNLLSLRILTLDGNNLIS--TSIWEFPLLSSLQDLTSITLSNNP 925          

HSP 3 Score: 114.005 bits (284), Expect = 6.990e-26
Identity = 146/581 (25.13%), Postives = 252/581 (43.37%), Query Frame = 0
Query:  181 NLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLE--NSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQ------FSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF---------------------------------------RFGK----------LQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRR------IRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETL 698
            NL +  F +   +    + G P++ I P    +    L  LS      L  ++ R  E  + L +L+LS N L  +P   F     +  L L+ N LQ      FSP+      +  +++L L       +P + +  +  L T+ LSS N    V  + F +L   L+ L+L ++ L ++  + F   P +  + L NN +  +    F  +   L      N +    L    F SL  L +LDLS+N I SL       +T LQ L L  N ++T   GTF SQ N  L IL L+ N ++ +   +F                                       RF +          L+ L L  N IK++ + SF  +  L  L L  N I  + +  F+ +  +Q L+LA+N+++SL    F+ +  L  L +D   N+L    ++ +        +  L++S N + + +  F   +  +L++L++  N I     +++G   +      ++  D SHN I  +E  +F  + ++++  LNHNR+  L   + R++ +L+ VDLSHN+L+ L
Sbjct:   94 NLRSGHFGHLPTLKELRIQGCPMKKI-PSGSFQGLGGLTSLSVVTQDSLELDSERAFEGLDHLRSLNLSHNNLWSVPPGTFCRLSGLSTLNLSFNRLQDMHELSFSPSKRSTSCKLPLENLILSDNSYVEIPSQALSHVSKLETLELSS-NAINLVQDNAFDNLR-DLKYLSLANNQLIALPPHTFAPTPHLRELSLQNNSLGVLAPKIFSGLSRLLVLNVSRNEIGNEWLTPALFASLVRLVSLDLSHNRISSLDPSVLRPLTSLQILDLSHNLLTTLDSGTFLSQNN--LHILRLNHNDLKSLHARSFDGLSVVSSINLDSNALSALEENAFLDCCSTLNDLTLSNNRFSRVPDGLQGLDGLKTLDLGGNNIKEIHNGSFTGLRALYGLKLSSNGITNISESIFKPIPGVQVLNLAFNKIKSLGQGVFNKLRNLKMLRLD--GNEL----IDINGLLSGQEELQWLNVSGNRLQWFDYAF---IPKNLELLDIHGNQI-----ENLGNYXKLTDQFELKTXDXSHNKIKKLETLSFPKSIQVAN--LNHNRIRLLTPGMFRDKIKLRKVDLSHNYLKRL 653          

HSP 4 Score: 98.9821 bits (245), Expect = 3.165e-21
Identity = 115/425 (27.06%), Postives = 200/425 (47.06%), Query Frame = 0
Query:  500 LDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHL-AIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQND------LKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTS-LEIFKVAHNRLY-EIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQL----SSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKS 911
            L S  F  +  L  L ++G  ++++P  +FQ L  L  L +     +SL   +  +   L HL +++LSHN L ++      T+  LS + +L+LS+N +  +    F P +        L+ L LS N   EI    +  + ++  L+LS N I+ ++D  F     L  L L +N+L +L          L+ + L +N L  L  ++F   S L +  V+ N +  E    AL      L  LDLS N+I+++  S L  +  L  L+L  N ++T+D+  F    NL  L L  N L  +H  +F G+   +  +N+ + +L+ L         S+L  L +S N+ + +P + L  L  ++ LDL GN +    + ++  +  L  L LS N I  +    F  +  ++ L+++  + KS
Sbjct:   95 LRSGHFGHLPTLKELRIQGCPMKKIPSGSFQGLGGLTSLSVVTQ--DSLELDSERAFEGLDHLRSLNLSHNNLWSVP---PGTFCRLSGLSTLNLSFNRLQDMHELSFSPSKRSTSCKLPLENLILSDNSYVEIPSQALSHVSKLETLELSSNAINLVQDNAFDNLRDLKYLSLANNQLIALPPHTFAPTPHLRELSLQNNSLGVLAPKIFSGLSRLLVLNVSRNEIGNEWLTPALFASLVRLVSLDLSHNRISSLDPSVLRPLTSLQILDLSHNLLTTLDSGTFLSQNNLHILRLNHNDLKSLHARSFDGL-SVVSSINLDSNALSALEENAFLDCCSTLNDLTLSNNRFSRVP-DGLQGLDGLKTLDLGGNNIKEIHNGSFTGLRALYGLKLSSNGITNISESIFKPIPGVQVLNLAFNKIKS 512          

HSP 5 Score: 88.5817 bits (218), Expect = 4.183e-18
Identity = 160/686 (23.32%), Postives = 285/686 (41.55%), Query Frame = 0
Query:   72 LRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNII-QIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLET-------------------------QIKLLDLSNCLIQELASNALRGLEN-----------------------SLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGF---RYTIKDLNLLGQ-----DMYYVP--LEEIGIMRNLRTVGLSSVNNYGSVTTDQF----ADLS----PSLEALNLVDS-DLTSIEHN--------AFKHVPSISSMDLSNNRIE-----------YIEENAFRE--------------VGNALQYLRMSNALHFRALP------NGPFRSLSALRTLDLSNNHIRS----------LPLDTFHKMTRLQHLYLQDN------EISTFKRGTFHSQANPSLAILDLSFNHIERVEY----DTFRF---GKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEIT 627
            LREL+L  N+L  +   IF  L RL  L++SRN I  +  + A    +  L  LDL+ N +  +    +  + SL++LDLS+N +  +    F+ Q +L  L+L  N++++L A +F     ++  +L  N L  +E +AFL+                           +K LDL    I+E+ + +  GL                          ++ L+L+ N+++ L + +F     ++ LRL+ N L     I  NG    +  ++ LN+ G      D  ++P  LE + I  N     + ++ NY  +T DQF     D S      LE L+   S  + ++ HN         F+    +  +DLS+N ++           Y E  + +E                  +++ +  N +    LP      +      S+   LD+ N H             +  D F    R+Q     D        ++  +    H    P L  +D +  +++   +    DT  F    ++  + LN++ I  +D+Q+F  +  L IL LE NQ+++L    F N+  LQ L L  NR+E ++   FD++ +L  L +D   N L +  +       +L ++ S+ LS NN    +  F +  QN    +  +R++ S+I+
Sbjct:  282 LRELSLQNNSLGVLAPKIFSGLSRLLVLNVSRNEIGNEWLTPALFASLVRLVSLDLSHNRISSLDPSVLRPLTSLQILDLSHNLLTTLDSGTFLSQNNLHILRLNHNDLKSLHARSFDGLSVVSSINLDSNALSALEENAFLDCCSTLNDLTLSNNRFSRVPDGLQGLDGLKTLDLGGNNIKEIHNGSFTGLRALYGLKLSSNGITNISESIFKPIPGVQVLNLAFNKIKSLGQGVFNKLRNLKMLRLDGNEL-----IDINGLLSGQEELQWLNVSGNRLQWFDYAFIPKNLELLDIHGN----QIENLGNYXKLT-DQFELKTXDXSHNKIKKLETLSFPKSIQVANLNHNRIRLLTPGMFRDKIKLRKVDLSHNYLKRLQISSFNIAPYSESTSIQEEPRPHFYMGHNPLHCDCEMEWFKNMNEMTQARLPIIMDYSSIQCHGDSSTSILDIPNEHFLCQYEAHCFSLCMCCDFFACDCRMQCPEGCDCYHDSSWSVNVIQCSXRHHALVPPLIPMDATNIYMDGNNFTGLLDTQSFIGRKRISSMFLNNSNIGGIDNQTFNGLMELKILHLEDNQVQRLEGYEFVNISSLQELYLHRNRIEYIHERTFDNLLSLRILTLD--GNNLISTSIWEFPLLSSLQDLTSITLS-NNPWSCKCEFVQKFQN---FIAENRDVFSDIS 951          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Match: EMLSAG00000003751 (supercontig:LSalAtl2s:LSalAtl2s201:72137:78450:1 gene:EMLSAG00000003751 transcript:EMLSAT00000003751 description:"maker-LSalAtl2s201-augustus-gene-0.5")

HSP 1 Score: 107.457 bits (267), Expect = 3.317e-24
Identity = 132/522 (25.29%), Postives = 234/522 (44.83%), Query Frame = 0
Query:  338 PSLEALNLVDSDL----TSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGN----ALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQ-----HLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNT-YPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISD--SQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNL--QLSSLKSLNVSFNQLTFI 840
            PS ++L +  SD+    + +  N F + P +  +D+   +I  I    F  + +    ++Q +          +    F  L++L  +DL+ N++ SLP   F  +  L+     H YLQD ++  F   T     N  L  LDLS+N +  +    F    +L+ LLLN+N + ++  Q    +  L IL+L  N++  LP ++      ++ L L  N + +L          L+ L   +     +      S   + +L+N+++LDLSYN  +++  +    ++  L+IL+L  N I+ ++ D      +++ L LSHN I  + D     +  L SL L+ N LTS+      N  +LQ + L++N L +LP        L  F+  H                 L+ LD+  NQI ++ +       ++ LV L L GN +  +  D F  + NL+ L+L +N    +    F  +   L+ L++ N  L  +  L    S L+ LN+S N + + 
Sbjct:   40 PSAKSLTVQCSDVFLYESILRTNHFGYRPDLKELDIQFCKIRRIPALTFSGLSDLKTLSIQSMNSEWPAMTMDIARDAFTGLNSLNFIDLTFNNLWSLPPAAFCSLHHLKTLNISHNYLQDADLVGFASPTDGLGCNVPLLSLDLSYNSLSTLPPRVFASLTQLKSLLLNNNDLTEIQDQGLDNLLCLEILNLSHNRLVTLPPDSLP--PSIKELHLQNNSITTLT--------PLNDLENLVLLXLSRNXLSEESXAIFSSLTNLLALDLSYNRFTYLSPSLLRGLET-LQILDLRYNAITTLSNDVFSHTPQLKALLLSHNVIQSMGDALRSTSASLQSLSLDFNDLTSVESTDFHNLTQLQDLALNNNLLMSLP--------LGFFESLH----------------ELRTLDVGENQIESLQNHSKPFKHLKSLVALRLAGNGLKILPEDVFHGLDNLQVLHLGNNQFSDLPQKIFNPLM-QLRVLSLDNNQLKDINGLLTAQSELQWLNISSNHIQWF 525          

HSP 2 Score: 99.3673 bits (246), Expect = 1.156e-21
Identity = 129/518 (24.90%), Postives = 231/518 (44.59%), Query Frame = 0
Query:  285 GFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNN-YGSVTTDQFADLSPSLEALNLVD---SDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKL----------------------DSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLR-TSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAF 775
            G+R  +K+L++    +  +P      + +L+T+ + S+N+ + ++T D   D    L +LN +D   ++L S+   AF  +  + ++++S+N                  YL+ ++ + F A P         L +LDLS N + +LP   F  +T+L+ L L +N+++  +     +     L IL+LS N +  +  D+     +++L L +N I  L                          F  +T LL L L  N+   L     + L  LQ LDL YN + +L+   F     L   A+ LSHN +Q++     +T  +L    SL L +N+++ VE T F  +   L+ L L+ N++  +       L  +R LD+  N I  +++                      H K  ++ K L  + L+ N L+ LPE++F    +L++  + +N+  ++P K  NP+   L+ L L  NQ+  I+     Q + L +LN+  N I   D   F
Sbjct:   65 GYRPDLKELDIQFCKIRRIPALTFSGLSDLKTLSIQSMNSEWPAMTMDIARDAFTGLNSLNFIDLTFNNLWSLPPAAFCSLHHLKTLNISHN------------------YLQDADLVGF-ASPTDGLGCNVPLLSLDLSYNSLSTLPPRVFASLTQLKSLLLNNNDLTEIQDQGLDNLL--CLEILNLSHNRLVTLPPDSLP-PSIKELHLQNNSITTLTPLNDLENLVLLXLSRNXLSEESXAIFSSLTNLLALDLSYNRFTYLSPSLLRGLETLQILDLRYNAITTLSNDVFSHTPQLK--ALLLSHNVIQSMGDALRSTSASLQ---SLSLDFNDLTSVESTDFHNL-TQLQDLALNNNLLMSLPLGFFESLHELRTLDVGENQIESLQN----------------------HSKPFKHLKSLVALRLAGNGLKILPEDVFHGLDNLQVLHLGNNQFSDLPQKIFNPLM-QLRVLSLDNNQLKDINGLLTAQSE-LQWLNISSNHIQWFDYAFF 530          

HSP 3 Score: 93.9745 bits (232), Expect = 5.643e-20
Identity = 111/403 (27.54%), Postives = 197/403 (48.88%), Query Frame = 0
Query:  535 LQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRS-NTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRN------IISEITP-DDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEI-PVKALN-----------------PVQSSLK---YLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDS--FAGLRHLESLDISG 906
            L+ LD+ + ++  +    F  +  L  L+I   +++  A+ ++ + + +  L+++  +DL++NN+  +    F  + + LK LN+S N      ++   +P D +G    +  LDLS+N +S +    F +  +L SL LN+N LT +  + L N   L++++LSHN L TLP +  L  S++   + +N +  + P+  L                   + SSL     LDLS N+ T +S S L  ++ L  L+L  N I+T+  D F   P L+ L L+ N +  +             G  + +TS         +SL+SL++ FN LT +      NL+Q+++L L+ N L S P   + S+  L +L +  N I  + N S  F  L+ L +L ++G
Sbjct:   70 LKELDIQFCKIRRIPALTFSGLSDLKTLSIQSMNSEWPAMTMDIARDAFTGLNSLNFIDLTFNNLWSLPPAAFCSLHH-LKTLNISHNYLQDADLVGFASPTDGLGCNVPLLSLDLSYNSLSTLPPRVFASLTQLKSLLLNNNDLTEIQDQGLDNLLCLEILNLSHNRLVTLPPD-SLPPSIKELHLQNNSITTLTPLNDLENLVLLXLSRNXLSEESXAIFSSLTNLLALDLSYNRFTYLSPSLLRGLETLQILDLRYNAITTLSNDVFSHTPQLKALLLSHNVIQSM-------------GDALRSTS---------ASLQSLSLDFNDLTSVESTDFHNLTQLQDLALNNNLLMSLPLGFFESLHELRTLDVGENQIESLQNHSKPFKHLKSLVALRLAG 448          

HSP 4 Score: 92.0485 bits (227), Expect = 2.482e-19
Identity = 117/452 (25.88%), Postives = 205/452 (45.35%), Query Frame = 0
Query:  455 TFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQ-----------SFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAF----DSIGT-LSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHI----------------------EDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRT-SLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRI--HLDTFGGIRDHLQGLNVANTSLTLLPN---LQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNEL 861
            T H    P L  LD+ F  I R+   TF     L+ L      I+ ++S+           +F  +  L  + L  N +  LP   F +LH L+ L++++N L+  +   F    D +G  +  L++DLS+N L  L       + +L+ + SL L+ N+++ ++    + +   L+ILNLS N +  + PD +     I+ L L +N I+ +                          F +   L +L L++NR T L   LLR  + LQ++DL +N + TL  ++F  T  L+   ++HN +  +   AL    +SL+ L L  N +T++  +  + +  L  L L  N + ++    F  +  L  L++  N +  +  H   F  ++  L  L +A   L +LP      L +L+ L++  NQ + +P      L Q+R L L  N+L
Sbjct:   61 TNHFGYRPDLKELDIQFCKIRRIPALTFSGLSDLKTL-----SIQSMNSEWPAMTMDIARDAFTGLNSLNFIDLTFNNLWSLPPAAFCSLHHLKTLNISHNYLQDADLVGFASPTDGLGCNVPLLSLDLSYNSLSTLP---PRVFASLTQLKSLLLNNNDLTEIQDQGLDNLLC-LEILNLSHNRLVTLPPDSLPP--SIKELHLQNNSITTLTPLNDLENLVLLXLSRNXLSEESXAIFSSLTNLLALDLSYNRFTYLSPSLLRGLETLQILDLRYNAITTLSNDVFSHTPQLKALLLSHNVIQSMG-DALRSTSASLQSLSLDFNDLTSVESTDFHNLTQLQDLALNNNLLMSLPLGFFESLHELRTLDVGENQIESLQNHSKPFKHLKS-LVALRLAGNGLKILPEDVFHGLDNLQVLHLGNNQFSDLPQKIFNPLMQLRVLSLDNNQL 499          

HSP 5 Score: 88.1965 bits (217), Expect = 4.237e-18
Identity = 147/583 (25.21%), Postives = 251/583 (43.05%), Query Frame = 0
Query:  225 LIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAF----REV---GNALQYLRMSNALHFRAL-----------PNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRF--GKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLA 787
            +  ++A +A  GL NSL  +DL+ N L  LP   F     ++ L ++ N LQ               D +L+G   +  P + +G    L ++ LS  N+  ++    FA L+  L++L L ++DLT I+     ++  +  ++LS+NR+  +  ++     +E+    N++  L   N L    L               F SL+ L  LDLS N    L       +  LQ L L+ N I+T     F     P L  L LS N I+ +  D  R     LQ L L+ N +  ++S  F  +T+L  L+L  N +  LP   F++LH L+ LD+  N++ESL                            N S  +  L ++++L L+ N +  +    F  + N L++L+L  N  S++       L ++RVL                         L++N+L  ++G LL  Q  LQ +++S N ++      F R S+E      NR+ ++       V++S ++  LS N      D ++  ++ +  L+   N+ + +D D   C    EQL L+
Sbjct:  109 MTMDIARDAFTGL-NSLNFIDLTFNNLWSLPPAAFCSLHHLKTLNISHNYLQ---------------DADLVG---FASPTDGLGCNVPLLSLDLS-YNSLSTLPPRVFASLT-QLKSLLLNNNDLTEIQDQGLDNLLCLEILNLSHNRLVTLPPDSLPPSIKELHLQNNSITTLTPLNDLENLVLLXLSRNXLSEESXAIFSSLTNLLALDLSYNRFTYLSPSLLRGLETLQILDLRYNAITTLSNDVF--SHTPQLKALLLSHNVIQSMG-DALRSTSASLQSLSLDFNDLTSVESTDFHNLTQLQDLALNNNLLMSLPLGFFESLHELRTLDVGENQIESLQ---------------------------NHSKPFKHLKSLVALRLAGNGLKILPEDVFHGLDN-LQVLHLGNNQFSDLPQKIFNPLMQLRVLS------------------------LDNNQLKDING-LLTAQSELQWLNISSNHIQWFDYAFFPR-SIEWLDAHENRIEDL-------VETSPEFF-LSQNPYHC--DCEMEYLKNINSLSRNPNRPAVMDLDDIKCTS--EQLFLS 601          

HSP 6 Score: 84.3445 bits (207), Expect = 5.462e-17
Identity = 127/487 (26.08%), Postives = 230/487 (47.23%), Query Frame = 0
Query:   96 LERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISI-------AHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQ--------NFEYINYTSLAGNPLEWIEPDAFLE-TQIKLLDLSNCLIQELASNALRGLEN--SLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGS--VTTDQFADLSPS----LEALNLVD---SDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMS-NALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAF 553
            L+ LD+    I +I ++ F +G+  L  L +  ++  + P +++         + SL  +DL+ N +  +    F    HL TL +  N +Q+     F         N   ++   L+ N L  + P  F   TQ+K L L+N  + E+     +GL+N   LE L+LS NRL  LP D      +I+ L L +N +             T+  LN L  +   +       +        SS+ N  +  ++ ++F  LSPS    LE L ++D   + +T++ ++ F H P + ++ LS+N I+ + + A R    +LQ L +  N L   ++ +  F +L+ L+ L L+NN + SLPL  F  +  L+ L + +N+I + +  +   +   SL  L L+ N ++ +  D F     LQ L L +N+   L  + F  + +L +LSL+ NQ++ + +        LQ L+++ N ++  ++A F
Sbjct:   70 LKELDIQFCKIRRIPALTF-SGLSDLKTLSI-QSMNSEWPAMTMDIARDAFTGLNSLNFIDLTFNNLWSLPPAAFCSLHHLKTLNISHNYLQDADLVGFASPTDGLGCNVPLLSL-DLSYNSLSTLPPRVFASLTQLKSLLLNNNDLTEIQD---QGLDNLLCLEILNLSHNRLVTLPPDSLP--PSIKELHLQNNSIT------------TLTPLNDL--ENLVLLXLSRNXLSEESXAIFSSLTNLLALDLSYNRFTYLSPSLLRGLETLQILDLRYNAITTLSNDVFSHTPQLKALLLSHNVIQSMGD-ALRSTSASLQSLSLDFNDL--TSVESTDFHNLTQLQDLALNNNLLMSLPLGFFESLHELRTLDVGENQIESLQNHSKPFKHLKSLVALRLAGNGLKILPEDVFHGLDNLQVLHLGNNQFSDLPQKIFNPLMQLRVLSLDNNQLKDI-NGLLTAQSELQWLNISSNHIQWFDYAFF 530          

HSP 7 Score: 79.7221 bits (195), Expect = 1.810e-15
Identity = 118/462 (25.54%), Postives = 199/462 (43.07%), Query Frame = 0
Query:   71 SLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQT-------GVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE-----------------------TQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENA--FREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKL 500
            SL  ++L  N L S+P   F  L  L+ L++S N +     + F +        VP L+ LDL+ N +  +P    A +  L+ L L+NN + ++ D      L L+ L L  N +  L  ++      I    L  N +  + P   LE                       T +  LDLS      L+ + LRGLE +L+ LDL  N +  L  D+F     ++ L L+ N++Q       +  R T   L  L  D                       N+  SV +  F +L+  L+ L L ++ L S+    F+ +  + ++D+  N+IE ++ ++  F+ + + +      N L  + LP   F  L  L+ L L NN    LP   F+ + +L+ L L +N++     G   +Q+   L  L++S NHI+  +Y  F    ++ L  ++NRI+ L
Sbjct:  123 SLNFIDLTFNNLWSLPPAAFCSLHHLKTLNISHNYLQDADLVGFASPTDGLGCNVPLLS-LDLSYNSLSTLPPRVFASLTQLKSLLLNNNDLTEIQDQGLDNLLCLEILNLSHNRLVTLPPDSLP--PSIKELHLQNNSITTLTPLNDLENLVLLXLSRNXLSEESXAIFSSLTNLLALDLSYNRFTYLSPSLLRGLE-TLQILDLRYNAITTLSNDVFSHTPQLKALLLSHNVIQ----SMGDALRSTSASLQSLSLDF----------------------NDLTSVESTDFHNLT-QLQDLALNNNLLMSLPLGFFESLHELRTLDVGENQIESLQNHSKPFKHLKSLVALRLAGNGL--KILPEDVFHGLDNLQVLHLGNNQFSDLPQKIFNPLMQLRVLSLDNNQLKDIN-GLLTAQS--ELQWLNISSNHIQWFDYAFFP-RSIEWLDAHENRIEDL 547          

HSP 8 Score: 61.6178 bits (148), Expect = 7.460e-10
Identity = 69/244 (28.28%), Postives = 110/244 (45.08%), Query Frame = 0
Query:   67 SLALSLRELNLAGNALTSVPE----------------------GIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLET--QIKLLDLSNCLIQELA--SNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFN 284
            SL  S++EL+L  N++T++                         IF  L  L  LDLS N    + S +   G+  L +LDL  N +  +     +H   L+ L LS+N I+ + D        L +L L  N++ ++ +  F N   +   +L  N L  + P  F E+  +++ LD+    I+ L   S   + L+ SL AL L+ N L+ LPED+F   D ++ L L +N     P   FN
Sbjct:  244 SLPPSIKELHLQNNSITTLTPLNDLENLVLLXLSRNXLSEESXAIFSSLTNLLALDLSYNRFTYL-SPSLLRGLETLQILDLRYNAITTLSNDVFSHTPQLKALLLSHNVIQSMGDALRSTSASLQSLSLDFNDLTSVESTDFHNLTQLQDLALNNNLLMSL-PLGFFESLHELRTLDVGENQIESLQNHSKPFKHLK-SLVALRLAGNGLKILPEDVFHGLDNLQVLHLGNNQFSDLPQKIFN 484          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Match: gi|14424439|sp|P12024.2|CHAO_DROME (RecName: Full=Chaoptin; AltName: Full=Photoreceptor cell-specific membrane protein; Flags: Precursor)

HSP 1 Score: 480.33 bits (1235), Expect = 3.217e-150
Identity = 319/886 (36.00%), Postives = 492/886 (55.53%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVA--DPFFIG---QLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQF-SPNITFNGFRYTIKDLNLLGQ---DMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDT-FRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRS----NTYPTL--SNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIP-VKALNPVQSSLKYLDLSANQITTISDSQL-NQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRH-LESLDISGVRAKSFQVRASD 918
            LG N I+ +  +  R L  SL+ L L  N ++ +    F  L  LE LDLS NN+ +I    F  G+P L  L L DN++ +IP+ ++  +KSLR LD+S+N I  ++  + + I    +L+LD L L  N+I+ L  N+F+ F+ +N T   GNP+  +  DAF   +I+ + +  C +  ++  A   L NSL+ LDLS N L +L   +F +FD +R + + DN ++   P  TFN   YT+  L+L G          L  +  MRN+R++ +S + +  SV  + F D    LE L +  + L+ I+ +AFKHV  +  +D S N I  IE +AF E+G++L  L+MS+     ALP  P R L++L+ LD SNNHI S+   +FH +  L+ L L DN I    +GTF    +  L  + L FNH+  +   T F    L++L L+DN+I K++ ++F+ +  L  LSL GN+I  L DE+FQNL +L+ LD+A+N+L + NF  FD +GTLS+L +++SHNQ++ L  N S    N +  +  SNI  LDLS+NNIS +   +F P +  L  L+L  N +   T D  G +  ++ LDLS+N I  ++   F    +L  ++  HN L+ +   + +  + L++VD SHN L  LP+ LF    +E   V+HN + +IP     +    +L  L LS N I+TI    L N+ + L YL++  N +  ID   F  +P L  L+L+ N   ++   +F G+ + L  L + N SL+ +P ++L  L+   + +N+L  IP     N+S +R LDLS N+LT+ P     ++ HL  L LSGNPI  + N+SF G+   LE LDIS  R   F+    D
Sbjct:  158 LGYNHITHIQHDSFRGLEDSLQTLILRENCISQLMSHSFSGLLILETLDLSGNNLFEIDPNVFVDGMPRLTRLLLTDNILSEIPYDALGPLKSLRTLDISHNVIWSLSGNETYEIKASTKLNLDNLHLEYNHIEVLPPNSFKYFDTVNRTFFDGNPIHTLREDAFKPARIREIYMRYCGLTNISPVAFDSLVNSLQILDLSGNNLTKLHHKLFNNFDVLRVISMRDNKIKIQKPTETFNAVHYTLLKLDLSGDRNDPTNLQTLRNMTRMRNMRSLSISRLGS-SSVGPEDFKDFGVELEDLQITRASLSGIQSHAFKHVRGLKRLDFSENGISSIENDAFHEIGHSLISLKMSHGYSGSALPAEPLRHLTSLQELDFSNNHISSMSDTSFHFLKNLRLLELHDNRIEQVLKGTFQGDIHSKLEEISLRFNHLTSISQHTFFDLEALRKLHLDDNKIDKIERRAFMNLDELEYLSLRGNKINNLADESFQNLPKLEILDMAFNQLPNFNFDYFDQVGTLSNLNVNVSHNQIRQLMYNSSWSGRNEHGGMYHSNIKILDLSHNNISIIHPGYFRPAEISLTHLHLGYNSLMNTTRDVFGNMPHLQWLDLSYNWIHELDFDAFKNTKQLQLVFFGHNYLSDIPQDIFKPVQGLRIVDFSHNHLRGLPDNLFYNGGMEKLDVSHNMMLKIPSSSLSSLAALTLCELHLSNNFISTIHSMDLSNKFRSLRYLDISYNYLLRIDDAVFATMPKLAVLDLSHNRDLKVMDKSFMGLENSLIKLGLENISLSTVPEIRLKYLREFRLGYNELPSIPQELAHNMSNLRMLDLSNNDLTNVPLMT-QALPHLRRLMLSGNPITSLNNNSFDGVNEDLEMLDISNFRLHYFEYGCLD 1041          

HSP 2 Score: 144.436 bits (363), Expect = 8.876e-34
Identity = 191/727 (26.27%), Postives = 316/727 (43.47%), Query Frame = 0
Query:  203 LEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIR-QLPDETFQNLH-RLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGA-ANKLSSLYLNHNRLTS-LHGKLLRNQKRLQVVDLSHNFLETLPEELF-LRTSLEIFKVAHNRLYEIPVKAL-NPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNL------QLSSLKSL--NVSFNQLTFIPPNTLAN---------LSQVRELDLSGNELT-SPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
            L  IEP     T +  L +S   + E+  +A  GLE SL  L L  N L E+P         +R L L  N +    + +F G   +++ L L    +  +       +  L T+ LS  NN   +  + F D  P L  L L D+ L+ I ++A   + S+ ++D+S+N I  +        GN    ++ S  L+              L  L L  NHI  LP ++F     +   +   N I T +   F       + +      +I  V +D+     LQ L L+ N + KL  + F     L ++S+  N+I+ Q P ETF  +H  L +LDL+ +R +  N                     LQ LR         + N+ SL +S    S V    F+    +L+ L ++R  +S I       +R ++ LD S N IS IE+  F    + L SL ++H    S L  + LR+   LQ +D S+N + ++ +  F    +L + ++  NR+ ++        + S L+ + L  N +T+IS      ++ L  L+L+ NKI  I+  AF  +  LE L+L  N ++ +  ++F     +L  L + + +   LPN       Q+ +L +L  NVS NQ+  +  N+  +          S ++ LDLS N ++   P     +   L  LHL  N ++    D F  + HL+ LD+S
Sbjct:   91 LREIEPYFLQSTGMYRLKISGNHLTEIPDDAFTGLERSLWELILPQNDLVEIPSKSLRHLQKLRHLDLGYNHITHIQHDSFRGLEDSLQTLILRENCISQLMSHSFSGLLILETLDLSG-NNLFEIDPNVFVDGMPRLTRLLLTDNILSEIPYDALGPLKSLRTLDISHNVIWSLS-------GNETYEIKASTKLN--------------LDNLHLEYNHIEVLPPNSFKYFDTVNRTFFDGNPIHTLREDAFKPARIREIYMRYCGLTNISPVAFDSL-VNSLQILDLSGNNLTKLHHKLFNNFDVLRVISMRDNKIKIQKPTETFNAVHYTLLKLDLSGDRNDPTN---------------------LQTLR-----NMTRMRNMRSLSISRLGSSSVGPEDFKDFGVELEDLQITRASLSGIQSHAFKHVRGLKRLDFSENGISSIENDAFHEIGHSLISLKMSHGYSGSALPAEPLRHLTSLQELDFSNNHISSMSDTSFHFLKNLRLLELHDNRIEQVLKGTFQGDIHSKLEEISLRFNHLTSISQHTFFDLEALRKLHLDDNKIDKIERRAFMNLDELEYLSLRGNKINNLADESF----QNLPKLEILDMAFNQLPNFNFDYFDQVGTLSNLNVNVSHNQIRQLMYNSSWSGRNEHGGMYHSNIKILDLSHNNISIIHPGYFRPAEISLTHLHLGYNSLMNTTRDVFGNMPHLQWLDLS 764          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Match: gi|14423663|sp|P82963.1|CHAO_TRICA (RecName: Full=Chaoptin; AltName: Full=Photoreceptor cell-specific membrane protein)

HSP 1 Score: 297.745 bits (761), Expect = 7.015e-87
Identity = 200/540 (37.04%), Postives = 309/540 (57.22%), Query Frame = 0
Query:  382 FREVGNALQYLRMSNALH--FRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYP------TLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQS-SLKYLDLSANQITTIS-DSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLD--TFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAG-LRHLESLDISGV 907
            F ++G++L  L++++AL    +  P+   + L+ L  LDLSNN +R++P ++FH +  L+ ++LQDN I    RGTF    +  L  +  SFN +  V+  TF    +L+Q+ L+DNRI+ L+ ++F+ +  L  L+L+GN+I  +  ETFQNL  L+ LDLAYN + SL+F  FD +G+L    +++SHN+L  L V  S  +        L NI  LDLS+NNI+ V   FF PV+  L  L L  N +   T D  G +  ++VLDLSHN +  ++  TF    KL  L  +HNR++ +   L R    L++VD SHN L +LP+ LF  T LE   V+HN L ++P+ +L+   + +L  LDLS N I+++S   QL + + L +L+L  N++  IDA  F  +P L  LNL  N    + ++  +F G+   L  LN+ N SL+ +P L   +L SL+++FN L  +      N+S +R L+L  N+L++ P    HS+  L  L L GNPI  + N S  G    LE L++  +
Sbjct:   10 FVDIGDSLLTLKLTHALSSSVQNFPSDAIKILNRLEELDLSNNRLRNVPDNSFHFLRSLKKVHLQDNTIEMIHRGTFQGDIHRDLTEVYFSFNSVRNVQQHTFADLIQLEQIHLDDNRIESLERRAFMNLKSLKRLNLKGNKIATIAYETFQNLPELEDLDLAYNSISSLDFNIFDQVGSLGMFHVNMSHNKLINLVVAPSVPFEQDTGLGGLQNIKVLDLSFNNITSVAKQFFRPVELSLMQLYLGHNKLLNATKDLFGNMPHLQVLDLSHNSLYELDFDTFRNTKKLQWLDTSHNRISEIPNDLFRFLGNLRIVDFSHNRLRSLPDNLFRETGLERLDVSHNLLGKLPLTSLSLASAQTLSELDLSWNSISSLSHGGQLARFKCLSWLDLSYNRLGQIDAGTFKGIPRLASLNLGHNSQLTLEINGLSFQGLEYTLLHLNLDNVSLSQVPALSTPNLLSLSLAFNSLPTVALEVAGNISSLRYLNLDYNDLSAVPIVT-HSLTELRHLSLEGNPITTLSNTSLLGAANQLEELNLKNI 548          

HSP 2 Score: 134.42 bits (337), Expect = 5.260e-31
Identity = 173/640 (27.03%), Postives = 293/640 (45.78%), Query Frame = 0
Query:  226 IQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNG-FRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGN-ALQYLRMSN--ALHFRALPNGPFR------SLSALRTLDLSNNHIRSLPLDTFHKMT-RLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFD--SIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVG---QLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLL------VYLNLEGNKI---------------------STIDADAFCCVP-NLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLP 820
            +Q   S+A++ L N LE LDLS NRL+ +P++ F    +++++ L DN ++     TF G     + ++      +  V       +  L  + L   N   S+    F +L  SL+ LNL  + + +I +  F+++P +  +DL+ N I  ++ N F +VG+  + ++ MS+   ++    P+ PF        L  ++ LDLS N+I S+    F  +   L  LYL  N++    +  F +   P L +LDLS N +  +++DTFR   KLQ L  + NRI ++ +  F  +  L I+    N++R LPD  F+    L+RLD+++N L  L   +    S  TLS L  DLS N + +L  +          +  LDLSYN +  ++   F+ +   L  LNL  N  S++T +  G   Q     +L L+ + +S  + P     N LS L L  N L ++  ++  N   L+ ++L +N L  +P      T L    +  N +  +   +L    + L+ L+L    +T +      ++Q L      VY N++   I                      T+D +     P  L+ L L+   L +I    F G+R     L V NTS+  +P
Sbjct:   30 VQNFPSDAIKIL-NRLEELDLSNNRLRNVPDNSFHFLRSLKKVHLQDNTIEMIHRGTFQGDIHRDLTEVYFSFNSVRNVQQHTFADLIQLEQIHLDD-NRIESLERRAFMNLK-SLKRLNLKGNKIATIAYETFQNLPELEDLDLAYNSISSLDFNIFDQVGSLGMFHVNMSHNKLINLVVAPSVPFEQDTGLGGLQNIKVLDLSFNNITSVAKQFFRPVELSLMQLYLGHNKLLNATKDLFGNM--PHLQVLDLSHNSLYELDFDTFRNTKKLQWLDTSHNRISEIPNDLFRFLGNLRIVDFSHNRLRSLPDNLFRETG-LERLDVSHNLLGKLPLTSLSLASAQTLSEL--DLSWNSISSL--SHGGQLARFKCLSWLDLSYNRLGQIDAGTFKGIPR-LASLNLGHN--SQLTLEINGLSFQGLEYTLLHLNLDNVSLSQVPALSTPNLLS-LSLAFNSLPTVALEVAGNISSLRYLNLDYNDLSAVPIVTHSLTELRHLSLEGNPITTLSNTSLLGAANQLEELNLKNIDLTVLESGAFCKMQPLRTLKIGVYRNIKNFNIPSILQFNDGLKNLEIHVTKDTDTLDNEMRGAWPLKLQNLTLSGKGLKKITGKMFQGVRSPTFHLTVRNTSIIKIP 655          

HSP 3 Score: 114.39 bits (285), Expect = 9.123e-25
Identity = 135/479 (28.18%), Postives = 232/479 (48.43%), Query Frame = 0
Query:   48 ERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNII--------QIHSMA-FQTGVPHLAVLDLADNLMGKIPF---ISIAHVKSLRVLDLSNNRIEKVADPFFIG-QLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLET-QIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEEN--AFREVGNALQYLRMSN-----------------ALHFRALPNGPFR---SLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQL 490
            E+  L DN+I  L R    +L  SL+ LNL GN + ++    F +L  LE LDL+ N+I         Q+ S+  F   + H  +++L   +   +PF     +  +++++VLDLS N I  VA  FF   +L L  L L  N + N T + F N  ++    L+ N L  ++ D F  T +++ LD S+  I E+ ++  R L N L  +D S NRL+ LP+++F +   + RL ++ N+L   P          +  L+L         L E+ +  N     +SS+++ G +   +F  LS     L+L  + L  I+   FK +P ++S++L +N    +E N  +F+ +   L +L + N                 +L F +LP        ++S+LR L+L  N + ++P+ T H +T L+HL L+ N I+T    +    AN    + +L+  +I+    ++  F K+Q L
Sbjct:  119 EQIHLDDNRIESLERRAFMNLK-SLKRLNLKGNKIATIAYETFQNLPELEDLDLAYNSISSLDFNIFDQVGSLGMFHVNMSHNKLINLV--VAPSVPFEQDTGLGGLQNIKVLDLSFNNITSVAKQFFRPVELSLMQLYLGHNKLLNATKDLFGNMPHLQVLDLSHNSLYELDFDTFRNTKKLQWLDTSHNRISEIPNDLFRFLGN-LRIVDFSHNRLRSLPDNLFRE-TGLERLDVSHNLLGKLP----------LTSLSLASAQT----LSELDLSWN----SISSLSHGGQLA--RFKCLS----WLDLSYNRLGQIDAGTFKGIPRLASLNLGHNSQLTLEINGLSFQGLEYTLLHLNLDNVSLSQVPALSTPNLLSLSLAFNSLPTVALEVAGNISSLRYLNLDYNDLSAVPIVT-HSLTELRHLSLEGNPITTLSNTSLLGAAN---QLEELNLKNIDLTVLESGAFCKMQPL 564          

HSP 4 Score: 85.5001 bits (210), Expect = 9.367e-16
Identity = 142/520 (27.31%), Postives = 228/520 (43.85%), Query Frame = 0
Query:   72 LRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPH-LAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDL------SNCLIQELAS--------NALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLS-----ALRTLDLSNNHIRSLP-LDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEM-----TRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAID 565
            L EL+L+ N L +VP+  F  L+ L+++ L  N I  IH   FQ  +   L  +  + N +  +   + A +  L  + L +NRIE +    F+    L  L L+ N I  +    FQN   +    LA N +  ++ + F   Q+  L +       N LI  + +          L GL+N ++ LDLS N +  + +  F      R + L+   L    N   N      KDL               G M +L+ + LS  N+   +  D F + +  L+ L+   + ++ I ++ F+ + ++  +D S+NR+  + +N FRE G  L+ L +S+ L    L   P  SLS      L  LDLS N I SL       +   L  L L  N +     GTF  +  P LA L+L  N    +E +   F  L+  LL+ N    LD+ S  ++       LL LSL  N +  +  E   N+  L+ L+L YN L ++      S+  L HL+++
Sbjct:   44 LEELDLSNNRLRNVPDNSFHFLRSLKKVHLQDNTIEMIHRGTFQGDIHRDLTEVYFSFNSVRNVQQHTFADLIQLEQIHLDDNRIESLERRAFMNLKSLKRLNLKGNKIATIAYETFQNLPELEDLDLAYNSISSLDFNIF--DQVGSLGMFHVNMSHNKLINLVVAPSVPFEQDTGLGGLQN-IKVLDLSFNNITSVAKQFF------RPVELSLMQLYLGHNKLLNA----TKDL--------------FGNMPHLQVLDLSH-NSLYELDFDTFRN-TKKLQWLDTSHNRISEIPNDLFRFLGNLRIVDFSHNRLRSLPDNLFRETG--LERLDVSHNL----LGKLPLTSLSLASAQTLSELDLSWNSISSLSHGGQLARFKCLSWLDLSYNRLGQIDAGTF--KGIPRLASLNLGHNSQLTLEINGLSFQGLEYTLLHLN----LDNVSLSQVPALSTPNLLSLSLAFNSLPTVALEVAGNISSLRYLNLDYNDLSAVPIVT-HSLTELRHLSLE 521          

HSP 5 Score: 71.2478 bits (173), Expect = 2.284e-11
Identity = 94/368 (25.54%), Postives = 161/368 (43.75%), Query Frame = 0
Query:   55 NQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDN-MLQFSPN-ITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLS 420
            N I+ ++++  R + LSL +L L  N L +  + +F ++  L+ LDLS N++ ++    F+     L  LD + N + +IP      + +LR++D S+NR+  + D  F           RE  ++ L          +++  L   PL  +   +     +  LDLS   I  L+          L  LDLS NRL ++    F     +  L L  N  L    N ++F G  YT+  LNL    +  VP        NL ++ L + N+  +V  +   ++S SL  LNL  +DL+++       +  +  + L  N I  +   +     N L+ L + N +    L +G F  +  LRTL + 
Sbjct:  233 NNITSVAKQFFRPVELSLMQLYLGHNKLLNATKDLFGNMPHLQVLDLSHNSLYELDFDTFRN-TKKLQWLDTSHNRISEIPNDLFRFLGNLRIVDFSHNRLRSLPDNLF-----------RETGLERLD---------VSHNLLGKLPLTSLSLAS--AQTLSELDLSWNSISSLSHGGQLARFKCLSWLDLSYNRLGQIDAGTFKGIPRLASLNLGHNSQLTLEINGLSFQGLEYTLLHLNLDNVSLSQVPALSTP---NLLSLSL-AFNSLPTVALEVAGNIS-SLRYLNLDYNDLSAVPI-VTHSLTELRHLSLEGNPITTLSNTSLLGAANQLEELNLKN-IDLTVLESGAFCKMQPLRTLKIG 570          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Match: gi|75027275|sp|Q9VPF0.2|ATK_DROME (RecName: Full=Protein artichoke; Flags: Precursor)

HSP 1 Score: 195.282 bits (495), Expect = 1.021e-49
Identity = 212/772 (27.46%), Postives = 345/772 (44.69%), Query Frame = 0
Query:   78 AGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFIS--IAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLET-----------------------------QIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREV------GNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETL-PEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNV 811
             G+ LT +  G+F  L  L+ LDLS N +  IH  A  + +P+L  L L+ N +  +  +   +  ++ L+ L L NN I  + D  F+   +L  L L +N I  L   AF     +    L  N +  I P++ L+                              +++ LD+S  L+ EL   ALRG   +LE L L+ N L+ +  D  M    +R LR+ +N L     + F      +K L+L       V  + +  + +LR + LS  N    +  + F   +P LE LN+  ++LT I  +   H+  +  +D S N+++ +     R V      GN +  L  + +   + LPN        LR LDLS N I  LP   F     L+ L L  NE+   K               D SF  I+R+E           L L +N++ + D ++ + +  L  L+L+ N++  + D  F N  RL++LDL+ N + S++  AFD+  +L +L  DLS N L  + V   N    L+N+  +DLSYN IS ++       +N ++I  LS N+I E+       L +++ LDLS N I ++E       ++L    L  N+L  L   +      L      +N L  + PE      SL    +++N    +    L  ++ +L+ LDLS N +  +S   L  +  LV L ++ N+I  I    F  +P L  L++ +N L  I   TF  +R ++  L+V
Sbjct:  192 GGSGLTRLEAGLFDGLISLKNLDLSHNGLNWIHLRAL-SRLPNLVSLKLSHNQISDVGMVGRIVKDLEHLKKLRLDNNLITVIEDGSFVDLPNLSELHLNDNRITELQYGAFLRTPQLKTIYLQNNLIRRIHPESLLQASGSGVEAVHMYNNEIGHVEALRALLDALPRLRYLDMSGNLLSELPYGALRG-HGTLEQLHLNHNHLRLIERDALMAMPALRELRMRNNSLSSDLPLPFWNLP-GLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSE-NGLIELAPNSFRH-NPLLETLNISSNELTKIHSSTLIHLERLFEVDASYNQLKSVIAGLPRIVERISLKGNQITSLPAAASKDLQ-LPN--------LRMLDLSQNRIEQLPRHGFQGAMELRVLSLAQNELRQLK---------------DTSFIGIQRLEL----------LHLQENQLGEADERALLPLAELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRNLIRSISPTAFDTQRSLEYL--DLSGNALLDISVGLGN----LNNLRDIDLSYNQISRIQSDVIGGWRNVVEI-RLSNNLIVELQQGTFRNLPKLQYLDLSSNEIRNVEPGALKGLDELQEFVLADNKLVELKDHVFEELPSLLASHFQYNKLRYISPESFHNANSLVFLNLSNNHFRNMENIGLRSMR-NLEVLDLSTNGVKLVSTMPLKALNWLVELKMDNNQICRIQGSPFETMPRLRVLSMRNNQLRSIKERTFRNVRGNIAILDV 916          

HSP 2 Score: 147.517 bits (371), Expect = 9.717e-35
Identity = 220/917 (23.99%), Postives = 385/917 (41.98%), Query Frame = 0
Query:  107 IQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLD-----------------LSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLEN-----SLEALDLSANRLQELPEDIFMDFDTIRRLRL--NDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLS-----SVNNYGSVTTD--------------------QFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAF---------------REVGNA------------LQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLA--IDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETL-PEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLL--------PNLQLS-------------------SLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRA 916
            I I+ +  ++  P  A+L    +L GK   I  +H    +++D                 L NN++  +   FF G L +  L LR N+I+ ++       E          P     P   L   I +L ++      + S  L+ L +     SL  L +    LQELP  +F     ++ + +     + +    + F+G   ++K+L+L    + ++ L  +  + NL ++ LS      V   G +  D                     F DL P+L  L+L D+ +T +++ AF   P + ++ L NN I  I   +                 E+G+             L+YL MS  L    LP G  R    L  L L++NH+R +  D    M  L+ L +++N +S+     F +   P L  LDL+ N   RV+         L++L L++N + +L   SF     L  L++  N++ ++   T  +L RL  +D +YN+L+S+       I  L  +   I L  NQ+ +L    S     L N+  LDLS N I  +    F+    +L++L+L++N + ++       ++R+ +L L  N +   ++       +L +L L  N+L ++      N  RL+ +DLS N + ++ P     + SLE   ++ N L +I V   N   ++L+ +DLS NQI+ I    +   + +V + L  N I  +    F  +P L+ L+L+SN +  +      G+ D LQ   +A+  L  L        P+L  S                   SL  LN+S N    +    L ++  +  LDLS N +    +    +++ L+ L +  N I R+    F  +  L  L +   + +S + R 
Sbjct:   12 IGINLIRAESCPPSQAILPCRCSLRGKEIQIWCSHSNLPQIMDGLKAVERNIKGRIDELVLENNQLPALPGRFF-GSLQIVRLMLRHNSIERVSNGWLNELENGLVEIFVVEPQLRSIPAESLNGMINMLAIT------IQSEELKHLPDFSGLLSLTYLSVQTGALQELPSHLFRHLPKLQHIHITGGSGLTRLEAGL-FDGL-ISLKNLDLSHNGLNWIHLRALSRLPNLVSLKLSHNQISDVGMVGRIVKDLEHLKKLRLDNNLITVIEDGSFVDL-PNLSELHLNDNRITELQYGAFLRTPQLKTIYLQNNLIRRIHPESLLQASGSGVEAVHMYNNEIGHVEALRALLDALPRLRYLDMSGNL-LSELPYGALRGHGTLEQLHLNHNHLRLIERDALMAMPALRELRMRNNSLSSDLPLPFWNL--PGLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSENGLIELAPNSFRHNPLLETLNISSNELTKIHSSTLIHLERLFEVDASYNQLKSV-------IAGLPRIVERISLKGNQITSLPAAASKDL-QLPNLRMLDLSQNRIEQLPRHGFQGAM-ELRVLSLAQNELRQLKDTSFIGIQRLELLHLQENQLGEADERALLPLAELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRNLIRSISPTAFDTQRSLEYLDLSGNALLDISVGLGN--LNNLRDIDLSYNQISRIQSDVIGGWRNVVEIRLSNNLIVELQQGTFRNLPKLQYLDLSSNEIRNVEPGALKGL-DELQEFVLADNKLVELKDHVFEELPSLLASHFQYNKLRYISPESFHNANSLVFLNLSNNHFRNMENIGLRSMRNLEVLDLSTNGVKLVSTMPLKALNWLVELKMDNNQICRIQGSPFETMPRLRVLSMRNNQLRSIKERT 903          

HSP 3 Score: 126.331 bits (316), Expect = 3.753e-28
Identity = 166/614 (27.04%), Postives = 271/614 (44.14%), Query Frame = 0
Query:   45 GRGERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLG-QDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMS-NALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTF 655
            G  E+  L  N +  + R+ L ++  +LREL +  N+L+S     FW+L  L+ LDL++N   ++ S     G+P L  LDL++N + ++   S  H   L  L++S+N + K+     I    L  +    N ++++ A   +  E I   SL GN +  +   A  + Q+                       +L  LDLS NR+++LP   F     +R L L  N L+             +KD + +G Q +  + L+E               N  G         L+  L  LNL  + L +I  N F +   +  +DLS N I  I   AF +   +L+YL +S NAL   ++  G   +L+ LR +DLS N I  +  D       +  + L +N I   ++GTF +   P L  LDLS N I  VE    +   +LQ+ +L DN++ +L    F E+  LL    + N++R +  E+F N + L  L+L+ N     +F   ++IG             L+++R           N+  LDLS N +  V     + + N L  L +  N I  I       + R+RVL + +N +  I++ TF
Sbjct:  382 GTLEQLHLNHNHLRLIERDALMAMP-ALRELRMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFARVDSQLL-AGLPSLRRLDLSENGLIELAPNSFRHNPLLETLNISSNELTKIHSSTLIHLERLFEVDASYNQLKSVIAGLPRIVERI---SLKGNQITSLPAAASKDLQLP----------------------NLRMLDLSQNRIEQLPRHGFQGAMELRVLSLAQNELR------------QLKDTSFIGIQRLELLHLQE---------------NQLGEADERALLPLA-ELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRNLIRSISPTAF-DTQRSLEYLDLSGNALLDISVGLG---NLNNLRDIDLSYNQISRIQSDVIGGWRNVVEIRLSNNLIVELQQGTFRNL--PKLQYLDLSSNEIRNVEPGALKGLDELQEFVLADNKLVELKDHVFEELPSLLASHFQYNKLRYISPESFHNANSLVFLNLSNN-----HFRNMENIG-------------LRSMR-----------NLEVLDLSTNGVKLVSTMPLKAL-NWLVELKMDNNQICRIQGSPFETMPRLRVLSMRNNQLRSIKERTF 904          

HSP 4 Score: 120.553 bits (301), Expect = 2.149e-26
Identity = 163/658 (24.77%), Postives = 287/658 (43.62%), Query Frame = 0
Query:   72 LRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMA-FQTGVPHLAVLDLADNLMGKIPFIS--IAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE-TQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEI--GIMRNLRTVGLSSVNNYGSVTTDQFADLS-PSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGN----ALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELF--LRTSLEIFKVAHN 716
            L EL+L  N +T +  G F    +L+ + L  N I +IH  +  Q     +  + + +N +G +  +   +  +  LR LD+S N + ++      G   L+ L L  N+++ +  +A      +    +  N L    P  F     +K LDL+      + S  L GL  SL  LDLS N L EL  + F     +  L ++ N L    + T       I    L   D  Y  L+ +  G+ R +  + L   N   S+      DL  P+L  L+L  + +  +  + F+    +  + L+ N +  +++ +F  +       LQ  ++  A     LP      L+ LR L+L +N + ++  + F   +RL+ L L  N I +     F +Q   SL  LDLS N +  +         L+ + L+ N+I ++ S        ++ + L  N I +L   TF+NL +LQ LDL+ N + ++   A   +  L      L+ N+L  L   + + +  L ++++    YN + ++    F    N L  LNLS N    +    +  +R + VLDLS N +  +      A N L  L +++N++  + G       RL+V+ + +N L ++ E  F  +R ++ I  V  N
Sbjct:  284 LSELHLNDNRITELQYGAFLRTPQLKTIYLQNNLIRRIHPESLLQASGSGVEAVHMYNNEIGHVEALRALLDALPRLRYLDMSGNLLSELPYGALRGHGTLEQLHLNHNHLRLIERDALMAMPALRELRMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFARVDSQLLAGLP-SLRRLDLSENGLIELAPNSFRHNPLLETLNISSNELTKIHSSTL------IHLERLFEVDASYNQLKSVIAGLPRIVERISLKG-NQITSLPAAASKDLQLPNLRMLDLSQNRIEQLPRHGFQGAMELRVLSLAQNELRQLKDTSFIGIQRLELLHLQENQLGEADERALLP------LAELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRNLIRSISPTAFDTQR--SLEYLDLSGNALLDISVGLGNLNNLRDIDLSYNQISRIQSDVIGGWRNVVEIRLSNNLIVELQQGTFRNLPKLQYLDLSSNEIRNVEPGALKGLDELQEFV--LADNKLVEL---KDHVFEELPSLLASHFQYNKLRYISPESFHNA-NSLVFLNLSNNHFRNMENIGLRSMRNLEVLDLSTNGVKLVSTMPLKALNWLVELKMDNNQICRIQGSPFETMPRLRVLSMRNNQLRSIKERTFRNVRGNIAILDVDGN 919          

HSP 5 Score: 92.8189 bits (229), Expect = 7.212e-18
Identity = 98/343 (28.57%), Postives = 163/343 (47.52%), Query Frame = 0
Query:   46 RGERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQF-SPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGI--MRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREV 385
            R E   L +NQ+ E     L  LA  LR LNL  N L ++ +  F +  RLE+LDLSRN I  I   AF T    L  LDL+ N +  I  + + ++ +LR +DLS N+I ++      G  ++  ++L  N I  L    F+N   + Y  L+ N +  +EP                        AL+GL+  L+   L+ N+L EL + +F +  ++       N L++ SP    N       +L+    + ++  +E IG+  MRNL  + LS+ N    V+T     L+  +E L + ++ +  I+ + F+ +P +  + + NN++  I+E  FR V
Sbjct:  598 RLELLHLQENQLGEADERALLPLA-ELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRNLIRSISPTAFDTQR-SLEYLDLSGNALLDIS-VGLGNLNNLRDIDLSYNQISRIQSDVIGGWRNVVEIRLSNNLIVELQQGTFRNLPKLQYLDLSSNEIRNVEP-----------------------GALKGLD-ELQEFVLADNKLVELKDHVFEELPSLLASHFQYNKLRYISPESFHNANSLVFLNLS----NNHFRNMENIGLRSMRNLEVLDLST-NGVKLVSTMPLKALNWLVE-LKMDNNQICRIQGSPFETMPRLRVLSMRNNQLRSIKERTFRNV 907          

HSP 6 Score: 82.0333 bits (201), Expect = 1.624e-14
Identity = 150/620 (24.19%), Postives = 243/620 (39.19%), Query Frame = 0
Query:  437 RLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNH--IERVEYDTFRF--GKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSH--------NCISHIEDPTFGAANKLSSLYLNHNRLTSL-HGKLLRNQK---------------------------------------------------RLQVVDLSHNFLETLP-EELFLRTSLEIFKVAHNRLYEIPVKALNPVQS-----------------------SLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTF--------------------GGIRDHLQGLNVANTSLTLLP-----NLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGN-----------PIVRVMN-------------DSFAGLRHLESLDISGVRAKSFQVRASDC 919
            R+  L L++N++     G F      SL I+ L   H  IERV           L ++ + + +++ + ++S   M  +L ++++  +++ LPD  F  L  L  L +    L+ L    F  +  L H+ I         L    +  +  L ++ +LDLS+N ++++ +     + N L  L LS N IS     DVG + RI V DL H        N I+ IED +F     LS L+LN NR+T L +G  LR  +                                                   RL+ +D+S N L  LP   L    +LE   + HN L  I   AL  + +                        LK LDL+ NQ   +    L  +  L  L+L  N +  +  ++F   P LE LN++SN L +IH  T                      G+   ++ +++    +T LP     +LQL +L+ L++S N++  +P +      ++R L L+ NEL     T++  +  L  LHL  N           P+  + N             + F+    LE LD+S    +S    A D 
Sbjct:   66 RIDELVLENNQLPALP-GRFFG----SLQIVRLMLRHNSIERVSNGWLNELENGLVEIFVVEPQLRSIPAESLNGMINMLAITIQSEELKHLPD--FSGLLSLTYLSVQTGALQELPSHLFRHLPKLQHIHITGG----SGLTRLEAGLFDGLISLKNLDLSHNGLNWIHLRALSRLPN-LVSLKLSHNQIS-----DVGMVGRI-VKDLEHLKKLRLDNNLITVIEDGSFVDLPNLSELHLNDNRITELQYGAFLRTPQLKTIYLQNNLIRRIHPESLLQASGSGVEAVHMYNNEIGHVEALRALLDALPRLRYLDMSGNLLSELPYGALRGHGTLEQLHLNHNHLRLIERDALMAMPALRELRMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSENGLIELAPNSFRHNPLLETLNISSNELTKIHSSTLIHLERLFEVDASYNQLKSVIAGLPRIVERISLKGNQITSLPAAASKDLQLPNLRMLDLSQNRIEQLPRHGFQGAMELRVLSLAQNELRQLKDTSFIGIQRLELLHLQENQLGEADERALLPLAELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRNLIRSISPTAFDT 667          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Match: gi|2498124|sp|P70389.1|ALS_MOUSE (RecName: Full=Insulin-like growth factor-binding protein complex acid labile subunit; Short=ALS; Flags: Precursor)

HSP 1 Score: 151.369 bits (381), Expect = 5.528e-37
Identity = 153/485 (31.55%), Postives = 235/485 (48.45%), Query Frame = 0
Query:  309 GIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQ-LRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTS-LEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNP 790
            GI  + R + L   NN  S+ +  F +LS SL+ LNL  S L S+E  A   + ++  + L  N +  +    FR    +L  L + N L  R L  G FR LS L  L+L  N +  LP   F  +  L  L L  N+++  +           L  LDLS N +  V+ + F    +LQ+L L+ N I  +  ++F+ M  L  L L  N++  L ++TF  L  L  L LA+N + SL    F  +  L  L   L HN+++ L      T+  L  +  L L+ N I  V+V  F  + N + ++NLS N +  + P+ V Q L R+  L L H+C+  I   TF   + L  L+L  N ++S+  + L     L  +DL+ N L  LP +LF     LE   +++N+L  +    L P+Q +  +LDLS N++ T ++   + +  L YLNL  N + T     F   P LE+L L +NP
Sbjct:   71 GIPVSTRALWLDG-NNLSSIPSAAFQNLS-SLDFLNLQGSWLRSLEPQALLGLQNLYHLHLERNLLRSLAAGLFRHT-PSLASLSLGNNLLGR-LEEGLFRGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQPALLCGLG--ELRELDLSRNALRSVKANVFIHLPRLQKLYLDRNLITAVAPRAFLGMKALRWLDLSHNRVAGLLEDTFPGLLGLHVLRLAHNAITSLRPRTFKDLHFLEEL--QLGHNRIRQLG---EKTFEGLGQLEVLTLNDNQIHEVKVGAFFGLFN-VAVMNLSGNCLRSL-PEHVFQGLGRLHSLHLEHSCLGRIRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTANQLTHLPRQLFQGLGQLEYLLLSNNQLTMLSEDVLGPLQRAF-WLDLSHNRLETPAEGLFSSLGRLRYLNLRNNSLQT-----FVPQPGLERLWLDANP 536          

HSP 2 Score: 131.724 bits (330), Expect = 1.534e-30
Identity = 136/488 (27.87%), Postives = 219/488 (44.88%), Query Frame = 0
Query:  402 ALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTS-LEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPN---LQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPI 885
            ++P+  F++LS+L  L+L  + +RSL       +  L HL+L+ N + +   G F  +  PSLA L L  N + R+E   FR                        ++ L  L+L  N +  LPD  FQ L  L  L LA N+L  L  A    +G L  L  DLS N L++++ N     P L                              L L RN+I+ + P     ++ +R LDLSHN ++ + + TF     L  L L HN +TSL  +  ++   L+ + L HN +  L E+ F     LE+  +  N+++E+ V A   +  ++  ++LS N + ++ +     +  L  L+LE + +  I    F  +  L +L L  N +  I   +  G+ + L+ L++    LT LP      L  L+ L +S NQLT +  + L  L +   LDLS N L +P    + S+  L  L+L  N +
Sbjct:   88 SIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYHLHLERNLLRSLAAGLF--RHTPSLASLSLGNNLLGRLEEGLFR-----------------------GLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQPALLCGLGELREL--DLSRNALRSVKANVFIHLPRLQK----------------------------LYLDRNLITAVAPRAFLGMKALRWLDLSHNRVAGLLEDTFPGLLGLHVLRLAHNAITSLRPRTFKDLHFLEELQLGHNRIRQLGEKTFEGLGQLEVLTLNDNQIHEVKVGAFFGL-FNVAVMNLSGNCLRSLPEHVFQGLGRLHSLHLEHSCLGRIRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLE-LDLTANQLTHLPRQLFQGLGQLEYLLLSNNQLTMLSEDVLGPLQRAFWLDLSHNRLETPAEGLFSSLGRLRYLNLRNNSL 518          

HSP 3 Score: 131.339 bits (329), Expect = 1.708e-30
Identity = 150/559 (26.83%), Postives = 239/559 (42.75%), Query Frame = 0
Query:  171 TLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQ-IKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLET-LPEELFLRTSLEIFKVAHNRLYEIPVKAL 726
             L L  NN+ ++ + AFQN   +++ +L G+ L  +EP A L  Q +  L L   L++ LA+   R    SL +L L  N L  L E +F     +  L L  N L   P+  F G    + +L L G  + Y+    +  +  LR + LS                           + L S++ N F H+P +  + L  N I  +   AF                            + ALR LDLS+N +  L  DTF  +  L  L L  N I++ +  TF       L  L L  N I ++   TF   G+L+ L LNDN+I ++   +F  +  + +++L GN +R LP+  FQ L RL  L L ++ L  +    F  +  L  L   L  N + ++      +   LS ++ LDL+ N ++ +    F+ +   L+ L LS N ++ ++ D +G L+R   LDLSH                        NRL +    L  +  RL+ ++L +N L+T +P+    R  L+    A+      P+KAL
Sbjct:   78 ALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYHLHLERNLLRSLAAGLFRH-TPSLASLSLGNNLLGRLEEGLFRGLSHLWDLNLGWNSLVVLPDTVFQGLG-NLHELVLAGNKLTYLQPALLCGLGELRELDLSR--------------------------NALRSVKANVFIHLPRLQKLYLDRNLITAVAPRAFL--------------------------GMKALRWLDLSHNRVAGLLEDTFPGLLGLHVLRLAHNAITSLRPRTFKDLH--FLEELQLGHNRIRQLGEKTFEGLGQLEVLTLNDNQIHEVKVGAFFGLFNVAVMNLSGNCLRSLPEHVFQGLGRLHSLHLEHSCLGRIRLHTFAGLSGLRRLF--LRDNSISSIE---EQSLAGLSELLELDLTANQLTHLPRQLFQGL-GQLEYLLLSNNQLTMLSEDVLGPLQRAFWLDLSH------------------------NRLETPAEGLFSSLGRLRYLNLRNNSLQTFVPQPGLERLWLD----ANPWDCSCPLKAL 546          

HSP 4 Score: 127.487 bits (319), Expect = 3.228e-29
Identity = 137/508 (26.97%), Postives = 218/508 (42.91%), Query Frame = 0
Query:   67 SLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMS-NALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSH-LAIDLSHNQLQ 572
             + +S R L L GN L+S+P   F +L  L+ L+L  + +  +   A   G+ +L  L L  NL+  +      H  SL  L L NN + ++ +  F G  HL  L L  N++  L    FQ    ++   LAGN L +++P               C + E            L  LDLS N L+ +  ++F+    +++L L+ N++       F G                         M+ LR + LS  N    +  D F  L   L  L L  + +TS+    FK +  +  + L +NRI  + E  F  +G  L+ L ++ N +H   +  G F  L  +  ++LS N +RSLP   F  + RL  L+L+ + +   +  TF                              L++L L DN I  ++ QS   ++ LL L L  NQ+  LP + FQ L +L+ L L+ N+L  L   + D +G L     +DLSHN+L+
Sbjct:   71 GIPVSTRALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQAL-LGLQNLYHLHLERNLLRSLAAGLFRHTPSLASLSLGNNLLGRLEEGLFRGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQPALL------------CGLGE------------LRELDLSRNALRSVKANVFIHLPRLQKLYLDRNLITAVAPRAFLG-------------------------MKALRWLDLSH-NRVAGLLEDTFPGLL-GLHVLRLAHNAITSLRPRTFKDLHFLEELQLGHNRIRQLGEKTFEGLGQ-LEVLTLNDNQIH--EVKVGAFFGLFNVAVMNLSGNCLRSLPEHVFQGLGRLHSLHLEHSCLGRIRLHTFAG-------------------------LSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTANQLTHLPRQLFQGLGQLEYLLLSNNQLTML---SEDVLGPLQRAFWLDLSHNRLE 495          

HSP 5 Score: 120.939 bits (302), Expect = 4.102e-27
Identity = 119/428 (27.80%), Postives = 205/428 (47.90%), Query Frame = 0
Query:  488 QQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRT-SLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLT---LLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKS 911
            + L L+ N +  + S +F  ++ L  L+L+G+ +R L  +    L  L  L L  N L SL    F    +L+ L+  L +N L  L       +  LS++  L+L +N++  +  T F+ + N L  L L+ N ++ + P  +  L  +R LDLS N +  ++   F    +L  LYL+ N +T++  +     K L+ +DLSHN +  L E+ F     L + ++AHN +  +  +    +   L+ L L  N+I  + +     +  L  L L  N+I  +   AF  + N+  +NL+ N L  +    F G+   L  L++ ++ L    L     LS L+ L +  N ++ I   +LA LS++ ELDL+ N+LT  P   +  +  L  L LS N +  +  D    L+    LD+S  R ++
Sbjct:   77 RALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYHLHLERNLLRSLAAGLFRHTPSLASLS--LGNNLLGRLE---EGLFRGLSHLWDLNLGWNSLVVLPDTVFQGLGN-LHELVLAGNKLTYLQPALLCGLGELRELDLSRNALRSVKANVFIHLPRLQKLYLDRNLITAVAPRAFLGMKALRWLDLSHNRVAGLLEDTFPGLLGLHVLRLAHNAITSLRPRTFKDLHF-LEELQLGHNRIRQLGEKTFEGLGQLEVLTLNDNQIHEVKVGAFFGLFNVAVMNLSGNCLRSLPEHVFQGL-GRLHSLHLEHSCLGRIRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTANQLTHLPRQLFQGLGQLEYLLLSNNQLTMLSEDVLGPLQRAFWLDLSHNRLET 496          

HSP 6 Score: 85.1149 bits (209), Expect = 1.029e-15
Identity = 101/403 (25.06%), Postives = 162/403 (40.20%), Query Frame = 0
Query:   50 CALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE-TQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTF 451
            C LG+ +  +LSR  LR                 SV   +F  L RL++L L RN I  +   AF                +G         +K+LR LDLS+NR+  + +  F G L L  L+L  N I +L    F++  ++    L  N +  +    F    Q+++L L++  I E+   A  GL N +  ++LS N L+ LPE +F     +  L L  + L                                                  G +    FA LS  L  L L D+ ++SIE  +   +  +  +DL+ N++ ++    F+ +G  L+YL +SN      L       L     LDLS+N + +     F  + RL++L L++N + TF
Sbjct:  215 CGLGELRELDLSRNALR-----------------SVKANVFIHLPRLQKLYLDRNLITAVAPRAF----------------LG---------MKALRWLDLSHNRVAGLLEDTFPGLLGLHVLRLAHNAITSLRPRTFKDLHFLEELQLGHNRIRQLGEKTFEGLGQLEVLTLNDNQIHEVKVGAFFGLFN-VAVMNLSGNCLRSLPEHVFQGLGRLHSLHLEHSCL--------------------------------------------------GRIRLHTFAGLS-GLRRLFLRDNSISSIEEQSLAGLSELLELDLTANQLTHLPRQLFQGLGQ-LEYLLLSNN-QLTMLSEDVLGPLQRAFWLDLSHNRLETPAEGLFSSLGRLRYLNLRNNSLQTF 521          

HSP 7 Score: 59.6918 bits (143), Expect = 8.769e-8
Identity = 83/318 (26.10%), Postives = 139/318 (43.71%), Query Frame = 0
Query:  606 FEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRT-SLEIFKVAHN---RLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPN---LQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRA 916
            ++   ++L +   SRN+     PD +      R L L  N +S I    F   + L  L L  + L SL  + L   + L  + L  N L +L   LF  T SL    + +N   RL E   + L    S L  L+L  N +  + D+    +  L  L L GNK++ +     C +  L +L+L+ N L  +  + F  +   LQ L +    +T +     L + +L+ L++S N++  +  +T   L  +  L L+ N +TS     +  +  L  L L  N I ++   +F GL  LE L ++  +    +V A
Sbjct:   49 YDDYTDELSVFCSSRNLTQ--LPDGIPV--STRALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYHLHLERNLLRSLAAGLFRHTPSLASLSLGNNLLGRLEEGLFRGL----SHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQPALLCGLGELRELDLSRNALRSVKANVFIHL-PRLQKLYLDRNLITAVAPRAFLGMKALRWLDLSHNRVAGLLEDTFPGLLGLHVLRLAHNAITSLRPRTFKDLHFLEELQLGHNRIRQLGEKTFEGLGQLEVLTLNDNQIHEVKVGA 357          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Match: gi|543801|sp|P35859.1|ALS_RAT (RecName: Full=Insulin-like growth factor-binding protein complex acid labile subunit; Short=ALS; Flags: Precursor)

HSP 1 Score: 147.132 bits (370), Expect = 1.492e-35
Identity = 139/471 (29.51%), Postives = 225/471 (47.77%), Query Frame = 0
Query:  323 NNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQ-LRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTS-LEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNP 790
            NN  S+ +  F +LS SL+ LNL  S L S+E  A   + ++  + L  NR+  +    F             ++     L  G F+ LS L  L+L  N +  LP   F  +  L  L L  N+++  +   F       L  LDLS N +  V+ + F    +LQ+L L+ N I  +   +F+ M  L  L L  N++  L ++TF  L  L  L LA+N + SL    F  +  L  L   L HN+++ L      T+  L  +  L L+ N I+ V V  F  + N + ++NLS N +  + P+ V Q L ++  L L H+C+ H+   TF   + L  L+L  N ++S+  + L     L  +DL+ N L  LP +LF     LE   +++N+L  +  + L P+Q +  +LD+S N + T+++   + +  + YL+L  N + T         P LE+L L +NP
Sbjct:   84 NNLSSIPSAAFQNLS-SLDFLNLQGSWLRSLEPQALLGLQNLYYLHLERNRLRNLAVGLFTHT--PSLASLSLSSNLLGRLEEGLFQGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQPALFCGLG--ELRELDLSRNALRSVKANVFVHLPRLQKLYLDRNLITAVAPGAFLGMKALRWLDLSHNRVAGLMEDTFPGLLGLHVLRLAHNAIASLRPRTFKDLHFLEEL--QLGHNRIRQLG---ERTFEGLGQLEVLTLNDNQITEVRVGAFSGLFN-VAVMNLSGNCLRSL-PERVFQGLDKLHSLHLEHSCLGHVRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTTNRLTHLPRQLFQGLGHLEYLLLSYNQLTTLSAEVLGPLQRAF-WLDISHNHLETLAEGLFSSLGRVRYLSLRNNSLQTFSPQ-----PGLERLWLDANP 536          

HSP 2 Score: 134.42 bits (337), Expect = 1.960e-31
Identity = 141/466 (30.26%), Postives = 224/466 (48.07%), Query Frame = 0
Query:  240 SLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLG-QDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELF 703
            S  AL L  N L  +P   F +  ++  L L  + L+            +++   LLG Q++YY+ LE    +RNL  VGL +               +PSL +L+L  + L  +E   F+ +  +  ++L  N +  + +  F+ +GN  + +   N L +  L    F  L  LR LDLS N +RS+  + F  + RLQ LYL  N I+    G F      +L  LDLS N +  +  DTF     L  L L  N I  L  ++F ++  L  L L  N+IRQL + TF+ L +L+ L L  N++  +   AF  +  ++   ++LS N L++L       +  L  + SL L ++ +  V +  F  + + L+ L L  N IS I    +  L  +  LDL+ N ++H+    F     L  L L++N+LT+L  ++L   +R   +D+SHN LETL E LF
Sbjct:   75 STRALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLR------------SLEPQALLGLQNLYYLHLER-NRLRNL-AVGLFTH--------------TPSLASLSLSSNLLGRLEEGLFQGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTY--LQPALFCGLGELRELDLSRNALRSVKANVFVHLPRLQKLYLDRNLITAVAPGAFLGMK--ALRWLDLSHNRVAGLMEDTFPGLLGLHVLRLAHNAIASLRPRTFKDLHFLEELQLGHNRIRQLGERTFEGLGQLEVLTLNDNQITEVRVGAFSGLFNVA--VMNLSGNCLRSLP---ERVFQGLDKLHSLHLEHSCLGHVRLHTFAGL-SGLRRLFLRDNSISSIEEQSLAGLSELLELDLTTNRLTHLPRQLFQGLGHLEYLLLSYNQLTTLSAEVLGPLQRAFWLDISHNHLETLAEGLF 502          

HSP 3 Score: 118.242 bits (295), Expect = 3.464e-26
Identity = 117/423 (27.66%), Postives = 200/423 (47.28%), Query Frame = 0
Query:  488 QQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVN-RSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRT-SLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLT---LLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
            + L L+ N +  + S +F  ++ L  L+L+G+ +R L  +    L  L  L L  NRL +L      ++G  +H     S +    L        +  LS++  L+L +N++  +  T F+ + N L  L L+ N ++ + P     L  +R LDLS N +  ++   F    +L  LYL+ N +T++        K L+ +DLSHN +  L E+ F     L + ++AHN +  +  +    +   L+ L L  N+I  + +     +  L  L L  N+I+ +   AF  + N+  +NL+ N L  +    F G+ D L  L++ ++ L    L     LS L+ L +  N ++ I   +LA LS++ ELDL+ N LT  P   +  + HL  L LS N +  +  +    L+    LDIS
Sbjct:   77 RALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYYLHLERNRLRNL------AVGLFTHTPSLASLSLSSNLLGRLEEGLFQGLSHLWDLNLGWNSLVVLPDTVFQGLGN-LHELVLAGNKLTYLQPALFCGLGELRELDLSRNALRSVKANVFVHLPRLQKLYLDRNLITAVAPGAFLGMKALRWLDLSHNRVAGLMEDTFPGLLGLHVLRLAHNAIASLRPRTFKDLHF-LEELQLGHNRIRQLGERTFEGLGQLEVLTLNDNQITEVRVGAFSGLFNVAVMNLSGNCLRSLPERVFQGL-DKLHSLHLEHSCLGHVRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTTNRLTHLPRQLFQGLGHLEYLLLSYNQLTTLSAEVLGPLQRAFWLDIS 490          

HSP 4 Score: 113.62 bits (283), Expect = 8.722e-25
Identity = 136/532 (25.56%), Postives = 226/532 (42.48%), Query Frame = 0
Query:   68 LALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSH-LAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNI 598
            + +S R L L GN L+S+P   F +L  L+ L+L  + +  +   A   G+ +L  L L  N +  +      H  SL  L LS+N + ++ +  F G  HL  L L  N++  L    FQ    ++   LAGN L +++P  F            C + E            L  LDLS N L+ +  ++F+    +++L L+ N++       F G                         M+ LR + LS  N    +  D F  L   L  L L  + + S+    FK +  +  + L +NRI  + E  F  +G  L+ L +++      +  G F  L  +  ++LS N +RSLP   F  + +L  L+L+ + +   +  TF                              L++L L DN I  ++ QS   ++ LL L L  N++  LP + FQ L  L+ L L+YN+L +L   + + +G L     +D+SHN L+ L       + +L  +  L L  N++
Sbjct:   72 IPVSTRALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQAL-LGLQNLYYLHLERNRLRNLAVGLFTHTPSLASLSLSSNLLGRLEEGLFQGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQPALF------------CGLGE------------LRELDLSRNALRSVKANVFVHLPRLQKLYLDRNLITAVAPGAFLG-------------------------MKALRWLDLSH-NRVAGLMEDTFPGLL-GLHVLRLAHNAIASLRPRTFKDLHFLEELQLGHNRIRQLGERTFEGLGQ-LEVLTLNDN-QITEVRVGAFSGLFNVAVMNLSGNCLRSLPERVFQGLDKLHSLHLEHSCLGHVRLHTFAG-------------------------LSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTTNRLTHLPRQLFQGLGHLEYLLLSYNQLTTL---SAEVLGPLQRAFWLDISHNHLETL---AEGLFSSLGRVRYLSLRNNSL 518          

HSP 5 Score: 111.694 bits (278), Expect = 3.403e-24
Identity = 127/481 (26.40%), Postives = 213/481 (44.28%), Query Frame = 0
Query:  415 RTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQ------LLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTS-LEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNL---QLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPI 885
            R L L  N++ S+P   F  ++ L  L LQ + + +        +    L + +L + H+ER        G          L L+ N + +L+   F  ++ L  L+L  N +  LPD  FQ L  L  L LA N+L  L  A F  +G L  L  DLS N L++++ N                      FV +         L+ L L RN+I+ + P     ++ +R LDLSHN ++ + + TF     L  L L HN + SL  +  ++   L+ + L HN +  L E  F     LE+  +  N++ E+ V A + +  ++  ++LS N + ++ +     +  L  L+LE + +  +    F  +  L +L L  N +  I   +  G+ + L+ L++    LT LP      L  L+ L +S+NQLT +    L  L +   LD+S N L +     + S+  +  L L  N +
Sbjct:   77 RALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSL-------EPQALLGLQNLYYLHLERNRLRNLAVGLFTHTPSLASLSLSSNLLGRLEEGLFQGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQPALFCGLGELREL--DLSRNALRSVKAN---------------------VFVHLP-------RLQKLYLDRNLITAVAPGAFLGMKALRWLDLSHNRVAGLMEDTFPGLLGLHVLRLAHNAIASLRPRTFKDLHFLEELQLGHNRIRQLGERTFEGLGQLEVLTLNDNQITEVRVGAFSGL-FNVAVMNLSGNCLRSLPERVFQGLDKLHSLHLEHSCLGHVRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLE-LDLTTNRLTHLPRQLFQGLGHLEYLLLSYNQLTTLSAEVLGPLQRAFWLDISHNHLETLAEGLFSSLGRVRYLSLRNNSL 518          

HSP 6 Score: 71.2478 bits (173), Expect = 2.234e-11
Identity = 75/259 (28.96%), Postives = 113/259 (43.63%), Query Frame = 0
Query:   50 CALGDNQISELSREDLRSLALS-------LRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDL---SNCL-----------------------IQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNML 275
            C LG+ +  +LSR  LRS+  +       L++L L  N +T+V  G F  ++ L  LDLS N +  +    F  G+  L VL LA N +  +   +   +  L  L L +NRI ++ +  F G   L+ L L +N I  +   AF     +   +L+GN L  + P+   +   KL  L    +CL                       I  +   +L GL   LE LDL+ NRL  LP  +F     +  L L+ N L
Sbjct:  215 CGLGELRELDLSRNALRSVKANVFVHLPRLQKLYLDRNLITAVAPGAFLGMKALRWLDLSHNRVAGLMEDTF-PGLLGLHVLRLAHNAIASLRPRTFKDLHFLEELQLGHNRIRQLGERTFEGLGQLEVLTLNDNQITEVRVGAFSGLFNVAVMNLSGNCLRSL-PERVFQGLDKLHSLHLEHSCLGHVRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLE-LDLTTNRLTHLPRQLFQGLGHLEYLLLSYNQL 470          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Match: gi|2498123|sp|O02833.1|ALS_PAPHA (RecName: Full=Insulin-like growth factor-binding protein complex acid labile subunit; Short=ALS; Flags: Precursor)

HSP 1 Score: 141.354 bits (355), Expect = 1.190e-33
Identity = 143/502 (28.49%), Postives = 225/502 (44.82%), Query Frame = 0
Query:  402 ALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTS-LEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPN---LQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHL 899
            ++P   FR+LS+L  L+L    + SL       +  L HL+L+ N++ +   GTF     P+LA+L LS                       +NR+ +L+   F  +  L  L+L  N +  LPD  F+ L  L+ L LA NRL  L  A F  +  L  L  DLS N L+A++ N     P L                              L L RN+I+ + P     L+ +R LDLSHN ++ + + TF     L  L L+HN + SL  +   +   L+ + L HN +  L E  F     LE+  + HN+L E+ V A   + +++  ++LS N +  + +     +  L  L+LEG+ +  I    F  +  L +L L  N L  I   +  G+ + L+ L++ +  LT LP+     L  L+ L +S N+L  +P + L  L +   LD+S N L + P +   S+  L  L+L  N + R       GL  L
Sbjct:   88 SIPPAAFRNLSSLAFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLAVGTF--AYTPALALLGLS-----------------------NNRLSRLEDGLFEGLGNLWDLNLGWNSLAVLPDAAFRGLGGLRELVLAGNRLAYLQPALFSGLAELREL--DLSRNALRAIKANVFAQLPRLQK----------------------------LYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFEDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKVGAFLGL-TNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFAGLSGLRRLFLKDNGLVGIEEQSLWGLAELLE-LDLTSNQLTHLPHQLFQGLGKLEYLLLSHNRLAELPADALGPLQRAFWLDVSHNRLEALPGSLLASLGRLRYLNLRNNSL-RTFTPQPPGLERL 531          

HSP 2 Score: 127.102 bits (318), Expect = 4.600e-29
Identity = 131/470 (27.87%), Postives = 210/470 (44.68%), Query Frame = 0
Query:  171 TLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLE-------NSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVG 632
             L L  NN+ ++   AF+N   + + +L G  L  +EP A       LL L N     L  N LR L         +L  L LS NRL  L + +F     +  L L  N L   P+  F G    +++L L G  + Y+       +  LR + LS  N   ++  + FA L P L+ L L  + + ++   AF  + ++  +DLS+NR+  + E+ F  +          NA+   +L    F  L  L  L L +N IR L   +F  + +L+ L L  N++   K G F    N  +A+++LS N +  +    FR  GKL  L L  + + ++   +F  ++ L  L L+ N +  + +++   L  L  LDL  N+L  L    F  +G L +L   LSHN+L  L    ++    L     LD+S+N +  +  +    +   L+ LNL  N +   TP   G
Sbjct:   78 ALWLDSNNLSSIPPAAFRNLSSLAFLNLQGGQLGSLEPQA-------LLGLENLCHLHLERNQLRSLAVGTFAYTPALALLGLSNNRLSRLEDGLFEGLGNLWDLNLGWNSLAVLPDAAFRGLG-GLRELVLAGNRLAYLQPALFSGLAELRELDLSR-NALRAIKANVFAQL-PRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIA--SLRPRTFEDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKVGAFLGLTN--VAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFAGLSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQLTHLPHQLFQGLGKLEYLL--LSHNRLAEL---PADALGPLQRAFWLDVSHNRLEALPGSLLASL-GRLRYLNLRNNSLRTFTPQPPG 527          

HSP 3 Score: 124.02 bits (310), Expect = 4.249e-28
Identity = 140/574 (24.39%), Postives = 235/574 (40.94%), Query Frame = 0
Query:  236 GLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLG-QDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQG 808
            G+    +AL L +N L  +P   F +  ++  L L    L             +++   LLG +++ ++ LE                N   S+    FA  +P+L  L L ++ L+ +E   F+ + ++  ++L  N +  + + AFR +G   + +   N L +  L    F  L+ LR LDLS N +R++  + F ++ RLQ LYL  N I+    G F                              L+ L L+ NR+  L   +F  +  L +L L  N I  L   TF++LH L+ L L +NR+  L   +F+ +G L  L +D  HNQLQ ++V                      +F+ +T       ++ ++NLS N +  +       L ++  L L  +C+  I   TF   + L  L+L  N L  +  + L     L  +DL+ N L  LP +LF                    + L      L+YL LS N++  +    L  +Q   +L++  N++  +       +  L  LNL +N L        G  R  L+G
Sbjct:   71 GIPGGTQALWLDSNNLSSIPPAAFRNLSSLAFLNLQGGQLG------------SLEPQALLGLENLCHLHLER---------------NQLRSLAVGTFA-YTPALALLGLSNNRLSRLEDGLFEGLGNLWDLNLGWNSLAVLPDAAFRGLGGLRELVLAGNRLAY--LQPALFSGLAELRELDLSRNALRAIKANVFAQLPRLQKLYLDRNLIAAVAPGAF-------------------------LGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFEDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLD--HNQLQEVKVG---------------------AFLGLT-------NVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFAGLSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQLTHLPHQLF--------------------QGL----GKLEYLLLSHNRLAELPADALGPLQRAFWLDVSHNRLEALPGSLLASLGRLRYLNLRNNSLRTFTPQPPGLERLWLEG 535          

HSP 4 Score: 115.546 bits (288), Expect = 2.465e-25
Identity = 122/469 (26.01%), Postives = 211/469 (44.99%), Query Frame = 0
Query:   80 NALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLES 547
            N L+S+P   F +L  L  L+L    +  +   A   G+ +L  L L  N +  +   + A+  +L +L LSNNR+ ++ D  F G  +L  L L  N++  L   AF+    +    LAGN L +++P  F                        GL   L  LDLS N L+ +  ++F     +++L L+ N++       F G +  ++ L+L    +  + LE+         V   S N   S+    F DL   LE L L  + +  +   +F+ +  +  + L +N+++ ++  AF  + N        N L  R LP   FR L  L +L L  + +  +   TF  ++ L+ L+L+DN +   +  +    A   L  LDL+ N +  + +  F+  GKL+ LLL+ NR+ +L + +   + R   L +  N++  LP     +L RL+ L+L  N L +
Sbjct:   84 NNLSSIPPAAFRNLSSLAFLNLQGGQLGSLEPQAL-LGLENLCHLHLERNQLRSLAVGTFAYTPALALLGLSNNRLSRLEDGLFEGLGNLWDLNLGWNSLAVLPDAAFRGLGGLRELVLAGNRLAYLQPALF-----------------------SGLA-ELRELDLSRNALRAIKANVFAQLPRLQKLYLDRNLIAAVAPGAFLGLK-ALRWLDLSHNRVAGL-LEDTFPGLLGLRVLRLSHNAIASLRPRTFEDLH-FLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKVGAFLGLTNVAVMNLSGNCL--RNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFAGLSGLRRLFLKDNGLVGIEEQSLWGLA--ELLELDLTSNQLTHLPHQLFQGLGKLEYLLLSHNRLAELPADALGPLQRAFWLDVSHNRLEALPGSLLASLGRLRYLNLRNNSLRT 520          

HSP 5 Score: 106.301 bits (264), Expect = 1.998e-22
Identity = 113/400 (28.25%), Postives = 176/400 (44.00%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTF 451
            LG N ++ L     R L   LREL LAGN L  +   +F  L  L  LDLSRN +  I +  F   +P L  L L  NL+  +   +   +K+LR LDLS+NR+  + +  F G L L  L+L  N I +L    F++  ++    L  N                        I++LA  +  GL   LE L L  N+LQE+    F+    +  + L+ N L+  P   F G                      +G + +L   G    +  G +    FA LS  L  L L D+ L  IE  +   +  +  +DL++N++ ++    F+ +G  L+YL +S+      LP      L     LD+S+N + +LP      + RL++L L++N + TF
Sbjct:  177 LGWNSLAVLPDAAFRGLG-GLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFAQ-LPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFEDLHFLEELQLGHN-----------------------RIRQLAERSFEGL-GQLEVLTLDHNQLQEVKVGAFLGLTNVAVMNLSGNCLRNLPEQVFRG----------------------LGKLHSLHLEG----SCLGRIRPHTFAGLS-GLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQLTHLPHQLFQGLGK-LEYLLLSHN-RLAELPADALGPLQRAFWLDVSHNRLEALPGSLLASLGRLRYLNLRNNSLRTF 521          

HSP 6 Score: 87.8113 bits (216), Expect = 1.277e-16
Identity = 103/389 (26.48%), Postives = 164/389 (42.16%), Query Frame = 0
Query:  514 LSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESL 902
            L L+ N +  +P   F+NL  L  L+L   +L SL   A   +  L HL   L  NQL++L V      P L+    L LS N +S +E   FE + N L  LNL  N ++ +       L  +R L L+ N +++++   F    +L  L L+ N L ++   +     RLQ + L  N +  +    FL                           +L++LDLS N++  + +     +  L  L L  N I+++    F  +  LE+L L  N + ++   +F G                      L  L+ L +  NQL  +       L+ V  ++LSGN L + P   +  +  L SLHL G+ + R+   +FAGL  L  L
Sbjct:   79 LWLDSNNLSSIPPAAFRNLSSLAFLNLQGGQLGSLEPQALLGLENLCHL--HLERNQLRSLAVGTFAYTPALA---LLGLSNNRLSRLEDGLFEGLGN-LWDLNLGWNSLAVLPDAAFRGLGGLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFAQLPRLQKLYLDRNLIAAVAPGAFLGLK------------------------ALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFEDLHFLEELQLGHNRIRQLAERSFEG----------------------LGQLEVLTLDHNQLQEVKVGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFAGLSGLRRL 415          

HSP 7 Score: 83.5741 bits (205), Expect = 2.615e-15
Identity = 108/400 (27.00%), Postives = 174/400 (43.50%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE-TQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGF----RYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDN 446
            L  N+++ L       LA  LREL+L+ NAL ++   +F  L RL++L L RN I  +   AF                +G         +K+LR LDLS+NR+  + +  F G L L  L+L  N I +L    F++  ++    L  N +  +   +F    Q+++L L +  +QE+   A  GL N +  ++LS N L+ LPE +F     +  L L  + L      TF G     R  +KD  L+G                              +       L+  LE L+L  + LT + H  F+ +  +  + LS+NR+  +  +A   +  A  +L +S+     ALP     SL  LR L+L NN +R+       +   L+ L+L+ N
Sbjct:  201 LAGNRLAYLQPALFSGLA-ELRELDLSRNALRAIKANVFAQLPRLQKLYLDRNLIAAVAPGAF----------------LG---------LKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFEDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKVGAFLGLTN-VAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFAGLSGLRRLFLKDNGLVG------------------------------IEEQSLWGLAELLE-LDLTSNQLTHLPHQLFQGLGKLEYLLLSHNRLAELPADALGPLQRAF-WLDVSHN-RLEALPGSLLASLGRLRYLNLRNNSLRTF----TPQPPGLERLWLEGN 536          

HSP 8 Score: 83.5741 bits (205), Expect = 3.247e-15
Identity = 62/193 (32.12%), Postives = 102/193 (52.85%), Query Frame = 0
Query:  716 NRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQ---LSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
            N L  IP  A   + SSL +L+L   Q+ ++    L  ++ L +L+LE N++ ++    F   P L  L L++N L R+    F G+  +L  LN+   SL +LP+     L  L+ L ++ N+L ++ P   + L+++RELDLS N L +  +  +  +  L  L+L  N I  V   +F GL+ L  LD+S
Sbjct:   84 NNLSSIPPAAFRNL-SSLAFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLAVGTFAYTPALALLGLSNNRLSRLEDGLFEGL-GNLWDLNLGWNSLAVLPDAAFRGLGGLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFAQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLS 274          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Match: gi|122126327|sp|Q7KIN0.1|TOLL7_DROME (RecName: Full=Toll-like receptor 7; Flags: Precursor)

HSP 1 Score: 137.887 bits (346), Expect = 8.708e-32
Identity = 166/630 (26.35%), Postives = 295/630 (46.83%), Query Frame = 0
Query:   83 TSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLA--DNLMGKIPFI-----SIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSS---VNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNR------SNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLR---RIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHN 693
            +++P  +F  LQ LE L L    ++Q+ + AF+ G+  L  L L+  ++  G    +     S+  +K L  LDL +N + ++   F     +L  L L  N I+      F +      +  AG             +++++LD S+  ++ ++ +        L+ L+L+ N L EL  +      ++R + L++N L+  P   F G +  +++++L   ++Y +P      +  L  V LS     +N+   TT  FA L   L  LNL  + LT I++  FK +  +  ++L NN I +IE+NAF                    LP      L  L TL+L+ N + +L    F+ +  L  L L +N IS  +   F + ++  L  LDLS N +  V         L+ L L +N+I+  D+QSF  + +L  L L  NQI  +    FQ+L RL  L+LA NR++S+   +FD             + +L+A+R++R      +  + TL +++ L+LS N++ + +  F   + ++LK L++  N I  +   +  +L+   R++ LD SHN I+ I        N +  L++N+N + ++      ++  L  VDL  N
Sbjct:  123 STLPVAVFARLQTLEALRLDSCKLLQLPNNAFE-GLATLKSLRLSTHNSEWGPTRTLELFPDSLGGLKQLTDLDLGDNNLRQLPSGFLCPVGNLQVLNLTRNRIRTAEQMGFADMNCGAGSGSAG-------------SELQVLDASHNELRSISESWGISRLRRLQHLNLAYNNLSELSGEALAGLASLRIVNLSNNHLETLPEGLFAGSK-ELREIHLQQNELYELPKGLFHRLEQLLVVDLSGNQLTSNHVDNTT--FAGLI-RLIVLNLAHNALTRIDYRTFKELYFLQILNLRNNSIGHIEDNAF--------------------LP------LYNLHTLNLAENRLHTLDDKLFNGLYVLSKLTLNNNLISVVEPAVFKNCSD--LKELDLSSNQLNEVPRALQDLAMLRTLDLGENQIRTFDNQSFKNLHQLTGLRLIDNQIGNITVGMFQDLPRLSVLNLAKNRIQSIERGSFD------------KNFELEAIRLDRNFLADINGVFATLVSLLWLNLSENHLVWFDYAF---IPSNLKWLDIHGNYIEAL--GNYYKLQEEIRVKTLDASHNRITEIG--PMSIPNTIELLFINNNLIGNVQPNAFVDKANLARVDLYAN 687          

HSP 2 Score: 119.783 bits (299), Expect = 3.481e-26
Identity = 147/548 (26.82%), Postives = 238/548 (43.43%), Query Frame = 0
Query:  333 FADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKR--------GTFHSQANPSLAILDLSFNHIERVE--YDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNL--QLSSLKSLNVSFNQL-----TFIPPNTLANLSQVRELDLSGNELTS 863
            FA L  +LEAL L    L  + +NAF+ + ++ S+ LS +  E+          ++L  L+                    L  LDL +N++R LP      +  LQ L L  N I T ++        G     A   L +LD S N +  +   +   R  +LQ L L  N + +L  ++   +  L I++L  N +  LP+  F     L+ + L  N L  L    F  +  L  L +DLS NQL +  V+ + T+  L  ++ L+L++N ++ ++   F+ +   L+ILNL  N I  I  +    L  +  L+L+ N +  ++D  F     LS L LN+N ++ +   + +N   L+ +DLS N L  +P                        +AL  + + L+ LDL  NQI T  +     +  L  L L  N+I  I    F  +P L  LNLA N +  I   +F    + L+ + +    L  +  +   L SL  LN+S N L      FIP N       ++ LD+ GN + +
Sbjct:  130 FARLQ-TLEALRLDSCKLLQLPNNAFEGLATLKSLRLSTHNSEWGPTRTLELFPDSLGGLKQ-------------------LTDLDLGDNNLRQLPSGFLCPVGNLQVLNLTRNRIRTAEQMGFADMNCGAGSGSAGSELQVLDASHNELRSISESWGISRLRRLQHLNLAYNNLSELSGEALAGLASLRIVNLSNNHLETLPEGLFAGSKELREIHLQQNELYELPKGLFHRLEQL--LVVDLSGNQLTSNHVDNT-TFAGLIRLIVLNLAHNALTRIDYRTFKELYF-LQILNLRNNSIGHIEDNAFLPLYNLHTLNLAENRLHTLDDKLFNGLYVLSKLTLNNNLISVVEPAVFKNCSDLKELDLSSNQLNEVP------------------------RALQDL-AMLRTLDLGENQIRTFDNQSFKNLHQLTGLRLIDNQIGNITVGMFQDLPRLSVLNLAKNRIQSIERGSFDKNFE-LEAIRLDRNFLADINGVFATLVSLLWLNLSENHLVWFDYAFIPSN-------LKWLDIHGNYIEA 620          

HSP 3 Score: 100.908 bits (250), Expect = 2.347e-20
Identity = 134/531 (25.24%), Postives = 232/531 (43.69%), Query Frame = 0
Query:   53 GDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQ---------TGVPHLAVLD-------------------------LADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFE---------------YINYTSLAG-----------NPLEWIEPDAFLETQ-IKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVP--LEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHS-QANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGN 519
            G  +  EL  + L  L   L +L+L  N L  +P G    +  L+ L+L+RN I     M F          +    L VLD                         LA N + ++   ++A + SLR+++LSNN +E + +  F G   L  + L++N +  L    F   E               +++ T+ AG           N L  I+   F E   +++L+L N  I  +  NA   L N L  L+L+ NRL  L + +F     + +L LN+N++       F      +K+L+L    +  VP  L+++ ++R L  +G + +  + +     F +L   L  L L+D+ + +I    F+ +P +S ++L+ NRI+ IE  +F +    L+ +R+    +F A  NG F +L +L  L+LS NH+  +  D     + L+ L +  N I     G ++  Q    +  LD S N I  +   +     ++ L +N+N I  +   +FV+   L  + L  N
Sbjct:  173 GPTRTLELFPDSLGGLK-QLTDLDLGDNNLRQLPSGFLCPVGNLQVLNLTRNRIRTAEQMGFADMNCGAGSGSAGSELQVLDASHNELRSISESWGISRLRRLQHLNLAYNNLSELSGEALAGLASLRIVNLSNNHLETLPEGLFAGSKELREIHLQQNELYELPKGLFHRLEQLLVVDLSGNQLTSNHVDNTTFAGLIRLIVLNLAHNALTRIDYRTFKELYFLQILNLRNNSIGHIEDNAFLPLYN-LHTLNLAENRLHTLDDKLFNGLYVLSKLTLNNNLISVVEPAVFKNCS-DLKELDLSSNQLNEVPRALQDLAMLRTL-DLGENQIRTFDN---QSFKNLH-QLTGLRLIDNQIGNITVGMFQDLPRLSVLNLAKNRIQSIERGSF-DKNFELEAIRLDR--NFLADINGVFATLVSLLWLNLSENHL--VWFDYAFIPSNLKWLDIHGNYIEAL--GNYYKLQEEIRVKTLDASHNRITEIGPMSIP-NTIELLFINNNLIGNVQPNAFVDKANLARVDLYAN 687          

HSP 4 Score: 75.8702 bits (185), Expect = 1.225e-12
Identity = 148/658 (22.49%), Postives = 248/658 (37.69%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQ-------------------------TGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE-TQIKLLDLSNCLIQE---------------LASNALRGLENS-------LEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQ------FSPNITFNGFRYT---IKDLN------------------LLGQDMYYVP------------LEEIG----IMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNN----------RIE-----------YIEENAFREVGNALQYLRMSNALHFRALP----------------NGPFRSLSALRTLDL---SNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHI----ERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQAL 574
            L  N +SELS E L  LA SLR +NL+ N L ++PEG+F   + L  + L +N + ++    F                           G+  L VL+LA N + +I + +   +  L++L+L NN I  + D  F+   +L TL L EN +  L    F     ++  +L  N +  +EP  F   + +K LDLS+  + E               L  N +R  +N        L  L L  N++  +   +F D   +  L L  N +Q      F  N      R     + D+N                  L+  D  ++P            +E +G    +   +R   L + +N   +T      +  ++E L + ++ + +++ NAF    +++ +DL  N          R+            Y+  N F E    + +L+  N L  R  P                    R LS LR  D      +H  +L                 D E++     T +     S  ++D           RV  D+        + L+ N    L + +F+    L  L + G+Q+  + + TF +L  LQ L LA N+L +L+   F+ +  L  L   L +NQL  +
Sbjct:  279 LAYNNLSELSGEALAGLA-SLRIVNLSNNHLETLPEGLFAGSKELREIHLQQNELYELPKGLFHRLEQLLVVDLSGNQLTSNHVDNTTFAGLIRLIVLNLAHNALTRIDYRTFKELYFLQILNLRNNSIGHIEDNAFLPLYNLHTLNLAENRLHTLDDKLFNGLYVLSKLTLNNNLISVVEPAVFKNCSDLKELDLSSNQLNEVPRALQDLAMLRTLDLGENQIRTFDNQSFKNLHQLTGLRLIDNQIGNITVGMFQDLPRLSVLNLAKNRIQSIERGSFDKNFELEAIRLDRNFLADINGVFATLVSLLWLNLSENHLVWFDYAFIPSNLKWLDIHGNYIEALGNYYKLQEEIRVKTLDASHN--RITEIGPMSIPNTIELLFINNNLIGNVQPNAFVDKANLARVDLYANQLSKLQLQQLRVAPVVAPKPLPEFYLGGNPF-ECDCTMDWLQRINNLTTRQHPRVMDMANIECVMPHARGAAVRPLSGLRPQDFLCRYESHCFAL--------CHCCDFDACDCEMTCPSNCTCYHDQIWSTNVVDCGGQQTTELPRRVPMDS------SVVYLDGNNFPVLKNHAFIGRKNLRALYVNGSQVAAIQNRTFASLASLQLLHLADNKLRTLHGYEFEQLSALRELY--LQNNQLTTI 916          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Match: gi|543800|sp|P35858.1|ALS_HUMAN (RecName: Full=Insulin-like growth factor-binding protein complex acid labile subunit; Short=ALS; Flags: Precursor)

HSP 1 Score: 134.42 bits (337), Expect = 1.695e-31
Identity = 141/502 (28.09%), Postives = 227/502 (45.22%), Query Frame = 0
Query:  402 ALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTS-LEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNL---QLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHL 899
            ++P   F++LS+L  L+L    + SL       +  L HL+L+ N++ +   GTF     P+LA L LS                       +NR+ +L+   F  +  L  L+L  N +  LPD  F+ L  L+ L LA NRL  L  A F  +  L  L  DLS N L+A++ N                      FV++         L+ L L RN+I+ + P     L+ +R LDLSHN ++ + + TF     L  L L+HN + SL  +  ++   L+ + L HN +  L E  F     LE+  + HN+L E+   A   + +++  ++LS N +  + +     +  L  L+LEG+ +  I    F  +  L +L L  N L  I   +  G+ + L+ L++ +  LT LP+     L  L+ L +S N+L  +P + L  L +   LD+S N L + P++    +  L  L L  N + R       GL  L
Sbjct:   88 SVPPAAFQNLSSLGFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALGTFAH--TPALASLGLS-----------------------NNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGLGSLRELVLAGNRLAYLQPALFSGLAELREL--DLSRNALRAIKAN---------------------VFVQLP-------RLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGL-TNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFTGLSGLRRLFLKDNGLVGIEEQSLWGLAELLE-LDLTSNQLTHLPHRLFQGLGKLEYLLLSRNRLAELPADALGPLQRAFWLDVSHNRLEALPNSLLAPLGRLRYLSLRNNSL-RTFTPQPPGLERL 531          

HSP 2 Score: 130.183 bits (326), Expect = 4.737e-30
Identity = 130/470 (27.66%), Postives = 213/470 (45.32%), Query Frame = 0
Query:  171 TLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLE-------NSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVG 632
             L L  NN+ ++   AFQN   + + +L G  L  +EP A       LL L N     L  N LR L         +L +L LS NRL  L + +F    ++  L L  N L   P+  F G   ++++L L G  + Y+       +  LR + LS  N   ++  + F  L P L+ L L  + + ++   AF  + ++  +DLS+NR+  + E+ F  +          NA+   +L    F+ L  L  L L +N IR L   +F  + +L+ L L  N++   K G F    N  +A+++LS N +  +    FR  GKL  L L  + + ++   +F  ++ L  L L+ N +  + +++   L  L  LDL  N+L  L    F  +G L +L   LS N+L  L    ++    L     LD+S+N +  +  +   P+   L+ L+L  N +   TP   G
Sbjct:   78 ALWLDGNNLSSVPPAAFQNLSSLGFLNLQGGQLGSLEPQA-------LLGLENLCHLHLERNQLRSLALGTFAHTPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGL-GSLRELVLAGNRLAYLQPALFSGLAELRELDLSR-NALRAIKANVFVQL-PRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIA--SLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGLTN--VAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFTGLSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQLTHLPHRLFQGLGKLEYLL--LSRNRLAEL---PADALGPLQRAFWLDVSHNRLEALPNSLLAPL-GRLRYLSLRNNSLRTFTPQPPG 527          

HSP 3 Score: 120.939 bits (302), Expect = 3.835e-27
Identity = 133/503 (26.44%), Postives = 214/503 (42.54%), Query Frame = 0
Query:   73 RELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSH-LAIDLSHNQLQAL 574
            + L L GN L+SVP   F +L  L  L+L    +  +   A   G+ +L  L L  N +  +   + AH  +L  L LSNNR+ ++ D  F G   L  L L  N++  L   AF+    +    LAGN L +++P  F                        GL   L  LDLS N L+ +  ++F+    +++L L+ N++       F G                         ++ LR + LS  N    +  D F  L   L  L L  + + S+    FK +  +  + L +NRI  + E +F  +G  L+ L + +    + +  G F  L+ +  ++LS N +R+LP   F  + +L  L+L+ + +   +  TF                              L++L L DN +  ++ QS   +  LL L L  NQ+  LP   FQ L +L+ L L+ NRL  L     D++G L     +D+SHN+L+AL
Sbjct:   77 QALWLDGNNLSSVPPAAFQNLSSLGFLNLQGGQLGSLEPQAL-LGLENLCHLHLERNQLRSLALGTFAHTPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGLGSLRELVLAGNRLAYLQPALF-----------------------SGLA-ELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLG-------------------------LKALRWLDLSH-NRVAGLLEDTFPGLL-GLRVLRLSHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQ-LEVLTLDHN-QLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFTG-------------------------LSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQLTHLPHRLFQGLGKLEYLLLSRNRLAEL---PADALGPLQRAFWLDVSHNRLEAL 497          

HSP 4 Score: 119.398 bits (298), Expect = 1.367e-26
Identity = 131/491 (26.68%), Postives = 214/491 (43.58%), Query Frame = 0
Query:  415 RTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
            + L L  N++ S+P   F  ++ L  L LQ  ++     G+   QA   L + +L   H+ER                  N+++ L   +F     L  L L  N++ +L D  F+ L  L  L+L +N L  L  AAF  +G+L  L   L+ N+L  L+      +  L+ +  LDLS N +  ++   F  +   L+ L L RN+I+ + P     L+ +R LDLSHN ++ + + TF     L  L L+HN + SL  +  +        DL  +FLE L             ++ HNR+ ++  ++   +   L+ L L  NQ+  +       +  +  +NL GN +  +    F  +  L  L+L  + L RI   TF G                      LS L+ L +  N L  I   +L  L+++ ELDL+ N+LT  P   +  +  L  L LS N +  +  D+   L+    LD+S
Sbjct:   77 QALWLDGNNLSSVPPAAFQNLSSLGFLNLQGGQL-----GSLEPQA--LLGLENLCHLHLER------------------NQLRSLALGTFAHTPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGLGSLRELV--LAGNRLAYLQ---PALFSGLAELRELDLSRNALRAIKANVFVQLPR-LQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFK--------DL--HFLEEL-------------QLGHNRIRQLAERSFEGL-GQLEVLTLDHNQLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFTG----------------------LSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQLTHLPHRLFQGLGKLEYLLLSRNRLAELPADALGPLQRAFWLDVS 490          

HSP 5 Score: 105.531 bits (262), Expect = 3.213e-22
Identity = 113/400 (28.25%), Postives = 175/400 (43.75%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTF 451
            LG N ++ L     R L  SLREL LAGN L  +   +F  L  L  LDLSRN +  I +  F   +P L  L L  NL+  +   +   +K+LR LDLS+NR+  + +  F G L L  L+L  N I +L    F++  ++    L  N                        I++LA  +  GL   LE L L  N+LQE+    F+    +  + L+ N L+  P   F G                      +G + +L   G    +  G +    F  LS  L  L L D+ L  IE  +   +  +  +DL++N++ ++    F+ +G  L+YL +S       LP      L     LD+S+N + +LP      + RL++L L++N + TF
Sbjct:  177 LGWNSLAVLPDAAFRGLG-SLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVF-VQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFKDLHFLEELQLGHN-----------------------RIRQLAERSFEGL-GQLEVLTLDHNQLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRG----------------------LGKLHSLHLEG----SCLGRIRPHTFTGLS-GLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQLTHLPHRLFQGLGK-LEYLLLSRN-RLAELPADALGPLQRAFWLDVSHNRLEALPNSLLAPLGRLRYLSLRNNSLRTF 521          

HSP 6 Score: 59.3066 bits (142), Expect = 1.089e-7
Identity = 83/345 (24.06%), Postives = 134/345 (38.84%), Query Frame = 0
Query:  609 VQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLR-TSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIR---------DHLQGL-------------------NVAN------TSLTLLPNLQL----------------SSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESL 902
            V    + L L  N +S + P     L  +  L+L    +  +E         L  L+L  N+L SL      +   L  + LS+N L  L + LF    SL    +  N L  +P  A   +  SL+ L L+ N++  +  +  + +  L  L+L  N +  I A+ F  +P L++L L  N +  +    F G++         + + GL                    +A+        L  L  LQL                  L+ L +  NQL  +       L+ V  ++LSGN L + P   +  +  L SLHL G+ + R+   +F GL  L  L
Sbjct:   72 VPGGTQALWLDGNNLSSVPPAAFQNLSSLGFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALGTFAHTPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGL-GSLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFTGLSGLRRL 415          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Match: gi|45477220|sp|O88280.1|SLIT3_RAT (RecName: Full=Slit homolog 3 protein; Short=Slit-3; AltName: Full=Multiple epidermal growth factor-like domains protein 5; Short=Multiple EGF-like domains protein 5; Flags: Precursor)

HSP 1 Score: 124.405 bits (311), Expect = 1.483e-27
Identity = 214/893 (23.96%), Postives = 351/893 (39.31%), Query Frame = 0
Query:   68 LALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNI-----------TFNGFRYTIKDLNLLG------QDMYYV---PLEEIGIMRNLRTVGLSS--------VNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRG---------TFHSQANPSLAILDLSF-------NHIERVEYDTFRFGKLQQLLLNDNRIKK------------LDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYN---RLESLNFAAFDSIGTLSHLA----IDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYL-----NHNRLTSLHGKLLRNQKRLQVVDLSHN--FLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSL--KYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPN---LQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPI 885
               S   ++  G  L +VP GI    +  ERLDL RNNI +I  M F TG+ +L VL L DN +  I   +   +K L  L L+ N+++ + +  F     L  L L EN IQ +   AF+    +    L  N +  IE  AF                     ALR     LE L L+ N +  +    F     IR LRL+ N L    ++           T   F   +  ++L G      Q   YV   P  E     N  ++   S        V+  G   T+  A+L   +  + L  + + SI   AF     +  +D+S N+I  I  +AF+ + +    +   N +    +P G F  L +L+ L L+ N I  L ++TF  +  L  L L DN++ T  +G         T H   NP +    L +       N IE          +L    ++  + KK              S+ F+++        EG  +    D + Q L R+      Y    RL   + A  ++ G    L     I+LS+N+++ +   R   +   + +  L L+ N +  +    F  + + LK L L  N+IS +  D    L  +R+L L  N I+ I    F     LS++ L     N N   +  G+ LR ++ +          FL+ +P       +++ F    N      +    P Q +     +  S   + T+       +     L LEGN ++ +  +                      L TF      L  ++++N S+++L N     +S L +L +S+N+L  IP +    L  +R L L GN+++S P  +++ +  L  L L  NP+
Sbjct:   38 CTCSAASVDCHGLGLRAVPRGI---PRNAERLDLDRNNITRITKMDF-TGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAF--------------------RALR----DLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTIGQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPAC-NANSLSCPSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFIQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKI--TEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSSECFMDLVCPEKCRCEGTIV----DCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEV---REGAFDGAAGVQELMLTGNQLETMHGRMFRGL-SGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIP---IQDVAIQDFTCEGNEENSCQLSPRCPEQCTCVETVVRCSNRGLHTLPKGMPKDV---TELYLEGNHLTAVPKE----------------------LSTF----RQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGINPL 859          

HSP 2 Score: 85.8853 bits (211), Expect = 1.086e-15
Identity = 119/469 (25.37%), Postives = 194/469 (41.36%), Query Frame = 0
Query:  488 QQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHN--------------------------CISHIEDPTFGAANKLSSLYL-----------NHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQ-SSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRASD 918
            ++L L+ N I ++    F  +  L +L LE NQ+  +    FQ+L +L+RL L  N+L+ L    F S   L+ L  DLS NQ+Q +       +  ++ + +L L  N+IS +E   F  ++ DL+IL L+ N IS I       + +IR L L  N                          C++ +    F  A+     Y+           N N L+        N     +VD     L  +P    L   +   ++  N +  IP  A   +Q   LK +D+S NQI+ I+      ++ L  L L GNKI+ I    F  + +L+ L L +N ++ + ++TF      LQ LN+                  L++  N+L  I     A L  ++ L L+ N         W      L+ +L  NPI        +  R L +  IS +++K F+   S+
Sbjct:   64 ERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRL--DLSENQIQGI---PRKAFRGVTGVKNLQLDNNHISCIEDGAFRALR-DLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTIGQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANSLSCPSACSCSN----NIVDCRGKGLTEIPAN--LPEGIVEIRLEQNSIKSIPAGAF--IQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTF----QDLQNLNL------------------LSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKW------LADYLQDNPI-ETSGARCSSPRRLANKRISQIKSKKFRCSGSE 489          

HSP 3 Score: 83.9593 bits (206), Expect = 3.953e-15
Identity = 141/605 (23.31%), Postives = 241/605 (39.83%), Query Frame = 0
Query:  395 SNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHS----------------------QANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAF--------DSIG--TLSHLAIDL-----------------SHNQLQALRVNRSNTYPTLS----------------------NIMSLDLSYNNISFVEVTFFEPVQ-NDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLR-TSLEIFKVAHNRLYEIPVKALNPVQSSLKYLD--LSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQG--LNVANTSLTLLPN-------------------------LQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGL 896
             + L  RA+P G  R+      LDL  N+I  +    F  +  L+ L+L+DN++S  +RG F                        Q+ P L  LDLS N I+ +    FR    ++ L L++N I  ++  +F  +  L IL+L  N I ++   +F ++ +++ L L  N L      A+         +IG  TL    + L                  H++  A   N  +     S                       I+ + L  N+I  +    F  +Q   LK +++S+N IS+I PD    L+ +  L L  N I+ I    F     L  L LN N++  L     ++ + L ++ L  N L+T+ + LF    S++   +A N                LK+L   L  N I T S ++ +  +      L   +IS I +  F C  + +  N  S+      +D     +   +G  ++ +N  L+ +P+                          +L +L+ +N+S N++  +        + V+EL L+GN+L +     +  +  L +L L  N I  V ND+FAGL
Sbjct:   47 CHGLGLRAVPRGIPRNA---ERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTIGQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANSLSCPSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAF--IQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFV---------CDCHLKWLADYLQDNPIET-SGARCSSPR-----RLANKRISQIKSKKFRCSGSEDYRNRFSS---ECFMDLVCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGL 628          

HSP 4 Score: 62.003 bits (149), Expect = 2.144e-8
Identity = 90/349 (25.79%), Postives = 154/349 (44.13%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMS-NALH 399
            L DN I+ L    +     +LR++NL+ N +  V EG F     ++ L L+ N +  +H   F+ G+  L  L L  NL+  +   + A + S+R+L L +NRI  ++   F   + L T+ L  N        A+          ++GNP    +P  F   +I + D++   IQ+          N   +  LS       PE      +T+  +R ++  L   P     G    + +L L G  +  VP +E+   R L  + LS+ N+   +T   F+++S  L  L L  + L  I  +AF  + S+  + L  N I  + E +F ++  +L +L +  N LH
Sbjct:  539 LNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFR-GLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNP-RCQKP--FFLKEIPIQDVA---IQDFTCEG-----NEENSCQLSP----RCPEQCTC-VETV--VRCSNRGLHTLP----KGMPKDVTELYLEGNHLTAVP-KELSTFRQLTLIDLSN-NSISMLTNHTFSNMS-HLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDL-TSLSHLALGINPLH 860          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Match: gi|341942040|sp|Q9R1B9.2|SLIT2_MOUSE (RecName: Full=Slit homolog 2 protein; Short=Slit-2; Contains: RecName: Full=Slit homolog 2 protein N-product; Contains: RecName: Full=Slit homolog 2 protein C-product; Flags: Precursor)

HSP 1 Score: 124.02 bits (310), Expect = 2.179e-27
Identity = 194/832 (23.32%), Postives = 331/832 (39.78%), Query Frame = 0
Query:   48 ERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPL----------EWI--EPDAFLETQI-----------------------------KLLDLSNCLIQELASNAL---RG---------LENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNML-QFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRI----------EYIEENAFREVGNAL----------------QYLRMSNALHFRALPNG--------PFRSLSALRTLDLSNNHIRSLP----------------------LDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLR-TSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKI 767
            ER  L  N I+ +++ D   L   LR L L  N ++++  G F DL+ LERL L+RNN+     + F  G   L  LDL++N +  IP  +      ++ L L  N+I  + D  F     L+ L L  NNI  L+  +F +   +    L  N L          +W+   P   L TQ                                  + +C      SN +   RG         L  ++  + L  N ++ +P   F  +  +RRL L++N + + +P+  F G R ++  L L G  +  +P + +        + L + N    +  D F DL  +L  L+L D+ L ++    F  + +I +M L+ N            +Y+  N     G                   +  R S    +R+  +G        P +      T+D SN  +  +P                         F K+ +L+ +   +N+I+  + G F   +  +  +  L+ N +E V++  F+    L+ L+L  NRI  + + SF+ +  + +LSL  NQI  +    F +LH L  L+L  N         F+    L+ L   L   ++          Y  L  I   D++  + +       +   +D     LSR   SE T  D        V+  S+  +  +     G    ++ LYL+ N+ T L  K L N K L ++DLS+N + TL  + F   T L    +++NRL  IP +  + ++ SL+ L L  N I+ + +   N +  L +L +  N +
Sbjct:   58 ERLDLNGNNITRITKIDFAGLR-HLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLF-LGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPD-AFQGLR-SLNSLVLYGNKITELP-KSLFEGLFSLQLLLLNANKINCLRVDAFQDLH-NLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEIL--LTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLAN--------PFNCNCHLAWLGEWLRRKRIVTGNPRCQKPY-FLKEIPIQDVAIQDFT------CDDGNDDNSCSPLSR-CPSECTCLDT-------VVRCSNKGLKVLPK---GIPKDVTELYLDGNQFT-LVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLK-SLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPL 853          

HSP 2 Score: 109.383 bits (272), Expect = 6.456e-23
Identity = 206/912 (22.59%), Postives = 342/912 (37.50%), Query Frame = 0
Query:   68 LALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITF-NGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALH---FRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRL----------ESLNFAAFDSIGTLSHLAIDLSHNQLQALRVN--RSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQ-----------------------LRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKIST----------------IDADAFCCVPNLEQLNLASNPLHRIHLDTF----GGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNV--------------SFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISG 906
             + S   ++  G AL SVP  I    +  ERLDL+ NNI +I  + F  G+ HL VL L +N +  I   +   +K L  L L+ N ++   +  F+G   L  L L EN IQ +   AF+    I    L  N +  IE  AF                     ALR     LE L L+ N +  L    F     +R  RL+ N L    ++ + + +      + L  Q M            +LR   ++ V     V +   + ++PS   L                H P+  +   SNN ++        E+   L        L     R +P G F     LR LDLSNN I  L  D F  +  L  L L  N+I+   +  F                              LQ LLLN N+I  L   +F ++  L +LSL  N+++ +   TF  L  +Q + LA N            + L+    ++ G        L++ ++  ++    R +      + +S D       F ++   E  + +   ++ S   +++I PD + Q                       L ++R ++ S+N I+ IE+  F  A+ ++ + L  NRL ++  K+ +  + L+ + L  N +  +  + F+                           S++ L L  NQITT++    + +  L  LNL  N  +                 +  +  C  P      L   P+  + +  F    G   +    L+   +  T L  +   S K L V                NQ T + P  L+N   +  +DLS N +++  + ++ +M  LL+L LS N +  +   +F GL+ L  L + G
Sbjct:   32 CSCSGSTVDCHGLALRSVPRNI---PRNTERLDLNGNNITRITKIDF-AGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAF--------------------RALR----DLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMG---------PSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVL----------------HCPAACT--CSNNIVD-CRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLF-------------------------SLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDC------FADLACPEKCRCEGTTVDCSNQRLNKI-PDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGL------------------------GSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYF----LKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLV-PKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHG 826          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Match: gi|1325301348|ref|XP_023332215.1| (chaoptin-like, partial [Eurytemora affinis])

HSP 1 Score: 711.449 bits (1835), Expect = 0.000e+0
Identity = 390/759 (51.38%), Postives = 534/759 (70.36%), Query Frame = 0
Query:  150 LSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLS-HLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGV 907
            LS NRIE++ D F   ++ LD + L  N I  L  N F NF ++N+TSL GNPL  I+  AF E++I+ LDL NC I  +   A RGLE SL+ L+L++NRL  LP ++F DFD+IR++ L+DN+L  SPN TF+ FRY + DL+L G  M +VP  EIG++RNLR + L S+++YG++   QF D  P LE ++LV+SD+  I +NAF HVPS+SS+DLS N+I+ IEE AF+E+GN+L+ LRM +ALH   LPN PF++L +L  LDLS+NH+ S+PLDT HKM  L+ LYLQDN+++ F RGT HSQANP L ILDLSFNHI R+E+D FRF KL+ LL+NDNRI+ ++S++FVEM  L  L+LEGNQI  L DE+FQNLH LQ L+LAYN L S+NF + D +G+LS  L +DLSHN +  L  N+S  + + S I SLD+SYNNI+ ++  FFE + + L+ +N++ N I  +  + +G LR++ V+DLS+N I  IE+ T      L  L L +N L S+  K  R Q+ LQV+DLS N L+ LPE +F    LEIFK++ N L EIPVKALNPVQS+L++LDLS N I+ IS+SQL+QI+ LV L+L  N+I  ID+ AFCC+P L  L+L+ NPL     +   G+   L+ L+++NTSL+LLPNL L  L SLN+S N+LTF+P ++LAN+S +RELDLS N + SPP   W+ + +L SL LS NP+  + N+SF  L  L+ LD+ G+
Sbjct:    1 LSGNRIERIEDTFSKKRMRLDRMNLANNRILTLEKNEFSNFAWLNFTSLQGNPLHTIQDYAFSESEIRHLDLQNCQIGNILPAAFRGLERSLQELNLASNRLSILPYNVFEDFDSIRKIDLSDNVLAISPNYTFSEFRYALLDLDLSGDLMRHVPSREIGLLRNLRHLSLPSLHSYGNLRLSQFEDFPPGLEEISLVNSDIKVIRNNAFYHVPSVSSLDLSGNKIQRIEEIAFKEIGNSLRSLRMRHALHLTELPNQPFQNLVSLILLDLSDNHLVSIPLDTLHKMKNLRELYLQDNKLTGFMRGTLHSQANPELRILDLSFNHIRRIEHDMFRFEKLETLLINDNRIQTIESRAFVEMRALKHLNLEGNQIVSLNDESFQNLHSLQILNLAYNNLRSVNFDSLDYVGSLSPTLHLDLSHNNIPTLASNQSQRFSSNSRIRSLDISYNNITSIQPGFFETMSSSLRTINIAYNKIPRVNKETIGSLRKLVVMDLSNNLIDSIEENTLEPITNLQRLNLANNNLGSIKDKTFRMQRSLQVLDLSGNQLQELPEHIFQVCRLEIFKISSNLLMEIPVKALNPVQSTLRHLDLSRNNISLISESQLSQIRSLVVLDLSNNRIKEIDSRAFCCLPELVSLSLSHNPLQMFSDELLDGVGTQLEHLDLSNTSLSLLPNLNLPELISLNMSLNKLTFVPSSSLANMSNIRELDLSWNSMPSPPHNVWYILPYLASLSLSYNPMTSIKNESFLSLDRLQRLDLGGL 759          

HSP 2 Score: 166.392 bits (420), Expect = 2.192e-38
Identity = 227/806 (28.16%), Postives = 378/806 (46.90%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDL------SNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE--TQIKLLDLSNCL-IQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQ---HLYLQDNEISTFKRGTFHS-QANPSLAILDLSFNHIERVEYDTFRF--GKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQAL-----RVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRN-QKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQ 836
            L + QI  +     R L  SL+ELNLA N L+ +P  +F D   + ++DLS N +    +  F      L  LDL+ +LM  +P   I  +++LR L L       N R+ +  D F  G   L+ + L  ++I+ +  NAF +   ++   L+GN ++ IE  AF E    ++ L + + L + EL +   + L  SL  LDLS N L  +P D       +R L L DN L                                 G MR             G++ +      +P L  L+L  + +  IEH+ F+    + ++ +++NRI+ IE  AF E+  AL++L +       +L +  F++L +L+ L+L+ N++RS+  D+   +  L    HL L  N I T          +N  +  LD+S+N+I  ++   F      L+ + +  N+I +++ ++   + +L+++ L  N I  + + T + +  LQRL+LA N L S+    F    +L    +DLS NQLQ L     +V R   +   SN++           + V    PVQ+ L+ L+LSRN IS I+   + Q+R + VLDLS+N I  I+   F    +L SL L+HN L     +LL     +L+ +DLS N   +L   L L   + +  ++ N+L  +P  +L  + S+++ LDLS N + +   +    +  L  L+L  N +++I  ++F  +  L++L+L             GG    L+  NV + S   LPN     LK L +S NQ
Sbjct:   72 LQNCQIGNILPAAFRGLERSLQELNLASNRLSILPYNVFEDFDSIRKIDLSDNVLAISPNYTFSEFRYALLDLDLSGDLMRHVPSREIGLLRNLRHLSLPSLHSYGNLRLSQFED-FPPG---LEEISLVNSDIKVIRNNAFYHVPSVSSLDLSGNKIQRIEEIAFKEIGNSLRSLRMRHALHLTELPNQPFQNLV-SLILLDLSDNHLVSIPLDTLHKMKNLRELYLQDNKL--------------------------------TGFMR-------------GTLHSQA----NPELRILDLSFNHIRRIEHDMFR-FEKLETLLINDNRIQTIESRAFVEM-RALKHLNLEGN-QIVSLNDESFQNLHSLQILNLAYNNLRSVNFDSLDYVGSLSPTLHLDLSHNNIPTLASNQSQRFSSNSRIRSLDISYNNITSIQPGFFETMSSSLRTINIAYNKIPRVNKETIGSLRKLVVMDLSNNLIDSIEENTLEPITNLQRLNLANNNLGSIKDKTFRMQRSLQ--VLDLSGNQLQELPEHIFQVCRLEIFKISSNLL---------MEIPVKALNPVQSTLRHLDLSRNNISLISESQLSQIRSLVVLDLSNNRIKEIDSRAFCCLPELVSLSLSHNPLQMFSDELLDGVGTQLEHLDLS-NTSLSLLPNLNLPELISL-NMSLNKLTFVPSSSLANM-SNIRELDLSWNSMPSPPHNVWYILPYLASLSLSYNPMTSIKNESFLSLDRLQRLDL-------------GG----LELTNVESGSFATLPN-----LKHLTISVNQ 784          

HSP 3 Score: 78.9518 bits (193), Expect = 4.044e-11
Identity = 114/478 (23.85%), Postives = 206/478 (43.10%), Query Frame = 0
Query:   95 RLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLEN------------------------SLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGT-FHSQANPSLAILDLSF------NHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEM-TRLLILSLEGNQIRQLPDETFQNLHRLQRLDL 540
            R+  LD+S NNI  I    F+T    L  +++A N + ++   +I  ++ L V+DLSNN I+ + +       +L  L L  NN+ ++    F+    +    L+GN L+ +    F   ++++  +S+ L+ E+   AL  +++                        SL  LDLS NR++E+    F     +  L L+ N LQ   +   +G    ++ L+L    +  +P   +  + +L      S+N    V +   A++S ++  L+L  + + S  HN +  +P ++S+ LS N +  I+                          N  F SL  L+ LDL    + ++   +F  +  L+HL +  N+      G   HS  NP+L +L +        NH+     D+    KL+ +++    I  L    F  + +R L L+L  ++I  +  + F NL R Q L L
Sbjct:  439 RIRSLDISYNNITSIQPGFFETMSSSLRTINIAYNKIPRVNKETIGSLRKLVVMDLSNNLIDSIEENTLEPITNLQRLNLANNNLGSIKDKTFRMQRSLQVLDLSGNQLQELPEHIFQVCRLEIFKISSNLLMEIPVKALNPVQSTLRHLDLSRNNISLISESQLSQIRSLVVLDLSNNRIKEIDSRAFCCLPELVSLSLSHNPLQMFSDELLDGVGTQLEHLDLSNTSLSLLPNLNLPELISLNM----SLNKLTFVPSSSLANMS-NIRELDLSWNSMPSPPHNVWYILPYLASLSLSYNPMTSIK--------------------------NESFLSLDRLQRLDLGGLELTNVESGSFATLPNLKHLTISVNQDKLENLGDLLHS--NPALEVLHIHSNGASLKNHLSSSSPDSGLPDKLKHIIITSETITNLHPAVFKGLQSRELSLTLISDKI-LVERDLFLNLGRAQNLTL 882          

HSP 4 Score: 70.0922 bits (170), Expect = 2.105e-8
Identity = 113/446 (25.34%), Postives = 197/446 (44.17%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKS-LRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAF--LETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLN 493
            L +N I  +    L  +  +L+ LNLA N L S+ +  F   + L+ LDLS N + ++    FQ  V  L +  ++ NL+ +IP  ++  V+S LR LDLS N I  +++        L  L L  N I+ + + AF     +   SL+ NPL+    +    + TQ++ LDLSN  +  L +         L +L++S N+L  +P     +   IR L L+ N +   P                   +++Y       I+  L ++ L S N   S+  + F  L   L+ L+L   +LT++E  +F  +P++  + +S      + ++    +G+ L        LH  +  NG     ++L+      NH+ S   D+     +L+H+ +    I+      F    +  L++  +S   +  VE D F   G+ Q L LN
Sbjct:  494 LSNNLIDSIEENTLEPIT-NLQRLNLANNNLGSIKDKTFRMQRSLQVLDLSGNQLQELPEHIFQ--VCRLEIFKISSNLLMEIPVKALNPVQSTLRHLDLSRNNISLISESQLSQIRSLVVLDLSNNRIKEIDSRAFCCLPELVSLSLSHNPLQMFSDELLDGVGTQLEHLDLSNTSLSLLPNLN----LPELISLNMSLNKLTFVPSSSLANMSNIRELDLSWNSMPSPP------------------HNVWY-------ILPYLASLSL-SYNPMTSIKNESFLSLD-RLQRLDLGGLELTNVESGSFATLPNLKHLTIS------VNQDKLENLGDLLHSNPALEVLHIHS--NG-----ASLK------NHLSSSSPDSGLP-DKLKHIIITSETITNLHPAVFKGLQSRELSLTLISDKIL--VERDLFLNLGRAQNLTLN 883          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Match: gi|321458747|gb|EFX69810.1| (hypothetical protein DAPPUDRAFT_300683 [Daphnia pulex])

HSP 1 Score: 576.63 bits (1485), Expect = 0.000e+0
Identity = 337/863 (39.05%), Postives = 519/863 (60.14%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQ 913
            LG N I E++RED   +  SL+ L+LA N L ++    F   QRLERLDL  N+I+ +  +   +G   L+ L+LADN +  IPFIS+A ++SL  ++L+NNRI    D FF G++ +DTL L  N I NL   AFQNF  IN TSL GN +  I  DAF + +I+ L LS+C +  + + + RGLE+SL+ LDLS N L  LPE++   FD ++ L LNDN L F      +GFRYT++ +NL+G+ M  +P++++  +RNLR++GLSS+N+   V+   F     +LE L+L  + L +I  N+F+HVP +  +DLS NRI  IE +AF +VG +L +L ++N +    LP+ PF+ L AL+++DLSNN I +LP D FH M  ++ + LQDN I    +  F ++  P+L  + L+FN I  +E  TF     L+ L L DN+I ++   +F  +  L  +SLEGN I  +  E F NL +L+ L+L +N LE L+F   D +GTLS + +D+SHN +Q L  NR+  + + S+I SLDL YNNISF+   +FEP+++ L  L L  N +  I+ D    ++ +  LD+S N I  ++   F  A  L  L L+HN ++ ++  +L     L+V+++SHN L  LP+ LF  T LEI  V+HN++ +IP   L+ + ++L++LD S N+IT+I+  QL ++  LVYL+L  N IST+    F  +  L  L+L+SNP+  +    F  +   L  LN+A+     L +  L  L SLN+S+N +  +P +     + +++ D+S  +LT  P + W +   L SL+LSGN +  + N + A ++ LE L+I  +   +F+
Sbjct:  158 LGANLIVEVNREDFSGVEDSLQHLSLADNYLVTLQLESFTGFQRLERLDLRGNSILTVAPLG-SSGTLKLSHLNLADNALEHIPFISLAQMRSLNTINLANNRISTTFDVFFQGRISIDTLILDNNMIGNLPPFAFQNFNLINKTSLNGNLIREIAEDAFKDAKIRDLSLSDCSVHGVDARSFRGLESSLQRLDLSYNNLSTLPENLLDKFDFLKALILNDNPLTFKAEEVLSGFRYTLQTINLVGEKMGQIPVKQMNDIRNLRSLGLSSLND--RVSIGDFEGFGAALEHLSLSKNKLKTISSNSFRHVPGLKVLDLSENRISQIEADAFADVGTSLTHLHLTNGIGVGTLPSDPFKKLIALQSIDLSNNRITNLPDDFFHSMKEIRSINLQDNSIEKVPQQMFDNEHTPNLVNISLNFNFINAIEAQTFSDLPHLKILNLEDNKINRIAKGAFQNIESLEYISLEGNMINTIEAEAFHNLPKLETLNLGHNNLEKLSFDWLDQVGTLSAIKLDVSHNLIQQLSSNRTG-WSSYSSIRSLDLGYNNISFISRNYFEPIRSSLTHLVLQHNQLRNISRDVYSDMQHLLWLDISDNNIQLVDSDAFANAKSLQVLLLDHNDISEIYQDMLSRSSTLRVINISHNRLRFLPDTLFKDTQLEILDVSHNQISKIPDGCLSRIATTLRHLDASHNEITSITPDQLKKLTDLVYLDLSNNAISTLSEKTFSSLNRLSYLDLSSNPIQTVADHIFDNLLQSLVHLNLADIGSINLGDFHLPELLSLNISYNTVENLPSDFFTRYANLKDFDISYCQLTVLPESPWSTASKLRSLNLSGNNLTVMANGTLASMKSLEYLNIKNLPLHTFE 1016          

HSP 2 Score: 181.8 bits (460), Expect = 4.330e-43
Identity = 224/816 (27.45%), Postives = 362/816 (44.36%), Query Frame = 0
Query:  201 NPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFM---------------------DF----DTIRRLRLNDNMLQFSPNITFNGFR-----------------------YTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSL-SALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDL-----------SFNHI------------ERVEYDTFR-FG-KLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQ--------------------------LPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVV--DLSHNFLETLPEELFLRT------SLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPN--LQLSSLKSLNVSFNQLTFIPPNTLANL-SQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
            N L  +E +AF  T +  +D+ N  +  +   A  GLE SL  L L  NRLQ +P+                         DF    D+++ L L DN L      +F GF+                         +  LNL    + ++P   +  MR+L T+ L+  NN  S T D F     S++ L L ++ + ++   AF++   I+   L+ N I  I E+AF++    ++ L +S+      +    FR L S+L+ LDLS N++ +LP +   K   L+ L L DN + TFK     S    +L  ++L             N I            +RV    F  FG  L+ L L+ N++K + S SF  +  L +L L  N+I Q                          LP + F+ L  LQ +DL+ NR+ +L    F S+  +   +I+L  N ++ +     +   T  N++++ L++N I+ +E   F  + + LKILNL  N I+ I       +  +  + L  N I+ IE   F    KL +L L HN L  L    L     L  +  D+SHN ++ L      RT      S+    + +N +  I      P++SSL +L L  NQ+  IS    + +Q L++L++  N I  +D+DAF    +L+ L L  N +  I+ D        L+ +N+++  L  LP+   + + L+ L+VS NQ++ IP   L+ + + +R LD S NE+TS        +  L+ L LS N I  +   +F+ L  L  LD+S
Sbjct:   89 NGLRRVEENAFHRTGLWRIDIRNNHLYSVPEMAFAGLERSLGELYLPFNRLQRVPQKALQNLEKLKVLDLGANLIVEVNREDFSGVEDSLQHLSLADNYLVTLQLESFTGFQRLERLDLRGNSILTVAPLGSSGTLKLSHLNLADNALEHIPFISLAQMRSLNTINLA--NNRISTTFDVFFQGRISIDTLILDNNMIGNLPPFAFQNFNLINKTSLNGNLIREIAEDAFKDA--KIRDLSLSDC-SVHGVDARSFRGLESSLQRLDLSYNNLSTLPENLLDKFDFLKALILNDNPL-TFKAEEVLSGFRYTLQTINLVGEKMGQIPVKQMNDIRNLRSLGLSSLNDRVSIGDFEGFGAALEHLSLSKNKLKTISSNSFRHVPGLKVLDLSENRISQIEADAFADVGTSLTHLHLTNGIGVGTLPSDPFKKLIALQSIDLSNNRITNLPDDFFHSMKEIR--SINLQDNSIEKVPQQMFDNEHT-PNLVNISLNFNFINAIEAQTFSDLPH-LKILNLEDNKINRIAKGAFQNIESLEYISLEGNMINTIEAEAFHNLPKLETLNLGHNNLEKLSFDWLDQVGTLSAIKLDVSHNLIQQLSSN---RTGWSSYSSIRSLDLGYNNISFISRNYFEPIRSSLTHLVLQHNQLRNISRDVYSDMQHLLWLDISDNNIQLVDSDAFANAKSLQVLLLDHNDISEIYQDMLSR-SSTLRVINISHNRLRFLPDTLFKDTQLEILDVSHNQISKIPDGCLSRIATTLRHLDASHNEITSITPDQLKKLTDLVYLDLSNNAISTLSEKTFSSLNRLSYLDLS 890          

HSP 3 Score: 171.014 bits (432), Expect = 1.089e-39
Identity = 225/904 (24.89%), Postives = 421/904 (46.57%), Query Frame = 0
Query:   51 ALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQ-----TGVPH-LAVLDLADNLMGKIPFISIAHVKSLRVLDLS--NNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE--TQIKLLDLSNCL-IQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITF-NGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVG--NALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKM-TRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGK-LQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGT-LSHLAIDLSHNQLQALRVNR---------------------SNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQN---------------------DLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPE-ELFLRTSLEIFKVA-HNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVP-NLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVR--ELDLSGNELTS--PPSTAWHSMDH----LLSLHLSGNP 884
            +L D  +  +     R L  SL+ L+L+ N L+++PE +      L++ D  +  I+  + + F+     +G  + L  ++L    MG+IP   +  +++LR L LS  N+R+  + D    G   L+ L L +N ++ +++N+F++   +    L+ N +  IE DAF +  T +  L L+N + +  L S+  + L  +L+++DLS NR+  LP+D F     IR + L DN ++  P   F N     + +++L    +  +  +    + +L+ + L   N    +    F ++  SLE ++L  + + +IE  AF ++P + +++L +N +E +  +   +VG  +A++     N +   +     + S S++R+LDL  N+I  +  + F  + + L HL LQ N++    R  +    +  L  LD+S N+I+ V+ D F   K LQ LLL+ N I ++        + L ++++  N++R LPD  F++  +L+ LD+++N++  +       I T L HL  D SHN++ ++  ++                       T+ +L+ +  LDLS N I  V    F+ +                       +L  LN+S N +  +  D   +   ++  D+S+  ++ + +  +  A+KL SL L+ N LT +    L + K L+ +++ +  L T  E  LF  T+L    +  + R+  + +  L     ++K+L++  N    ISD  L  + +   L +E     T +      +P  L  + ++ N L      T GG                 L ++Q  SL +L +  N +T +      NL  VR  +LD+  N+L+S   PST  H        L  LH++ NP
Sbjct:  325 SLSDCSVHGVDARSFRGLESSLQRLDLSYNNLSTLPENL------LDKFDFLKALILNDNPLTFKAEEVLSGFRYTLQTINLVGEKMGQIPVKQMNDIRNLRSLGLSSLNDRVS-IGDFEGFGA-ALEHLSLSKNKLKTISSNSFRHVPGLKVLDLSENRISQIEADAFADVGTSLTHLHLTNGIGVGTLPSDPFKKLI-ALQSIDLSNNRITNLPDDFFHSMKEIRSINLQDNSIEKVPQQMFDNEHTPNLVNISLNFNFINAIEAQTFSDLPHLKILNLED-NKINRIAKGAFQNIE-SLEYISLEGNMINTIEAEAFHNLPKLETLNLGHNNLEKLSFDWLDQVGTLSAIKLDVSHNLIQQLSSNRTGWSSYSSIRSLDLGYNNISFISRNYFEPIRSSLTHLVLQHNQLRNISRDVYSDMQH--LLWLDISDNNIQLVDSDAFANAKSLQVLLLDHNDISEIYQDMLSRSSTLRVINISHNRLRFLPDTLFKDT-QLEILDVSHNQISKIPDGCLSRIATTLRHL--DASHNEITSITPDQLKKLTDLVYLDLSNNAISTLSEKTFSSLNRLSYLDLSSNPIQTVADHIFDNLLQSLVHLNLADIGSINLGDFHLPELLSLNISYNTVENLPSDFFTRYANLKDFDISYCQLTVLPESPWSTASKLRSLNLSGNNLTVMANGTLASMKSLEYLNIKNLPLHTFEEGSLFQMTNLRHLAIGTYERVKGMDIPKLLDFNHAIKHLEIDVNAEENISDG-LRDLHMKFLLQVEA---GTFENQLRGKLPFKLGNITISGNKLK-----TIGG---------------NALKDIQGKSL-TLTLRDNSVTELSRALFQNLGNVRWLQLDIRHNKLSSVAEPSTTIHPGTSGSVFLTQLHMADNP 1187          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Match: gi|1067081576|ref|XP_018024567.1| (PREDICTED: chaoptin-like [Hyalella azteca])

HSP 1 Score: 572.778 bits (1475), Expect = 0.000e+0
Identity = 338/864 (39.12%), Postives = 515/864 (59.61%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQV 914
            L  N I+ ++ ED       L  L +A N+L  +P+  F  L  L  LDL  NNI  I + AF+   P L  L+LA+NL+  IPF++ +++K L+ L+L  N I +  D  F+G L LDTL L  N I+ L  ++FQNF+ +N +S  GNPL+ I  DAF  ++I+ L L +C I  L+  + RG+E +LE LDLS N L ++PE+IF   D+++ L L  N L   P  +FNGF YT++ LN+LG+ M  +P++ +  MRN+RT   S+   +  ++ + F    P++E L L++++++S++  AF+HVP +  +DLS+N I   +  AF  VG  L+ LR+S+ L    LP  PF  L  LR LDLSNN +  L  D    M +L+HL L+DN+IS      F  Q  P L  + LSFN I  +E  TF     L+ + L+DN I  +   +F  +  L  LSL GN IR L  E FQN+ +++ LDL+YN + +LN  AF+ +GTLS L ID+SHN++ AL++N +  + ++S+I ++D SYNNIS ++  +FE V+  L+ L  S N +  ++ +   ++ +I++LD S+N IS ++   F   + L S+ L++N +  L   L +N + L  VDLS N +  +P++LF    +EI K++ N  + +P   L+ +  +L+ LDLS N +T ISD+ +  ++ L+ L++  NKI  I   +F  +  L  L++++NPL+ I L TF G++D LQ L+VANTS+   P LQ   L  LN S N L+F+P NT+ANL+ +R LD+S NELT+P S AW  M +L  L L GN +  + N +FA L  L+ LDI       FQ 
Sbjct:  178 LTGNHITSVAAEDFEGFNRYLVRLLMASNSLVEIPDEAFGPLSSLTELDLQDNNIRSIRNRAFEGASPTLRHLNLANNLLSSIPFMAFSYLKGLQYLNLERNLISQPYDVMFMGSLSLDTLILDFNAIEYLPPHSFQNFDSVNTSSFRGNPLQKIGADAFKGSKIRELYLQDCDIWNLSEKSFRGIEATLETLDLSYNNLTDIPENIFDRIDSLKWLSLAHNKLILDPKKSFNGFTYTLQYLNMLGEYMGIIPMDALKNMRNVRTFAFSTFPGF-YLSNEDFVGFGPAVEKLYLMNNEISSVKALAFEHVPGLKVLDLSHNVINSFDNRAFANVG-GLEELRVSSGLTLTRLPPQPFHYLINLRILDLSNNKLTFLQNDCLSHMKKLRHLNLEDNKISKLSSHQFRGQFTPELTTIQLSFNAIPVIESMTFHDLDHLKYIYLDDNMITSVKRFAFANLADLEYLSLVGNNIRDLEFEAFQNIPKVRHLDLSYNEMINLNLDAFEQVGTLSSLKIDVSHNKISALKMNGTR-WSSISSIKTIDFSYNNISSIDTEYFESVRISLQHLRFSHNNMYNLSANIFSKMLQIQLLDFSNNHISVVDPEAFRETDLLRSIDLSYNNIKDLPLTLFKNHRYLSYVDLSGNLMHRMPDDLFRLCPVEILKLSKNNFHGVPDNTLHHITETLRVLDLSWNSLTNISDAVIGGLENLIALDISHNKIHLIGYRSFHDLWRLTSLDVSNNPLNDIDLYTFDGLQDSLQNLSVANTSIKSFPELQHPKLAVLNASNNILSFLPTNTMANLTGIRTLDVSHNELTAPGSNAWQVMPYLRDLRLQGNFVRSITNSTFASLSRLQVLDIRDFPLHVFQA 1038          

HSP 2 Score: 167.548 bits (423), Expect = 1.233e-38
Identity = 215/863 (24.91%), Postives = 385/863 (44.61%), Query Frame = 0
Query:  131 MGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEY------INYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLS----------------------------------SVNNYGSVTTDQF---------AD---------------------------LSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKM-TRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDN-RIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLN--HNRLTSL--HGKLLRNQKRLQVVDLSHNFLETLPEELF--LRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPN--LQLSSLKSLNVSFNQLTFIPPNTLANLSQ-VRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
            +G++P   + +   +  L L +N++ ++ +  F G   L  L++R N++  +   AF           + Y  L+  P E ++       ++KLLDL+   I  +A+    G    L  L +++N L E+P++ F    ++  L L DN ++   N  F G   T++ LNL    +  +P      ++ L+ + L                                   S  N+ SV T  F         AD                           +  +LE L+L  ++LT I  N F  + S+  + L++N++    + +F      LQYL M    +   +P    +++  +RT   S      L  + F      ++ LYL +NEIS+ K   F  +  P L +LDLS N I   +   F   G L++L ++    + +L  Q F  +  L IL L  N++  L ++   ++ +L+ L+L  N++  L+   F    T     I LS N   A+ V  S T+  L ++  + L  N I+ V+   F  +  DL+ L+L  N I ++  +    + ++R LDLS+N + ++    F     LSSL ++  HN++++L  +G    +   ++ +D S+N + ++  E F  +R SL+  + +HN +Y                 +LSAN       S++ QIQLL + N   N IS +D +AF     L  ++L+ N +  + L  F   R +L  ++++   +  +P+   +L  ++ L +S N    +P NTL ++++ +R LDLS N LT+        +++L++L +S N I  +   SF  L  L SLD+S
Sbjct:   88 LGRVP--PVLNSSRVHTLTLRSNKLRQLDERSFYGSSGLWRLEIRHNDLFRIPEKAFLGIGRSLSELDLRYNELSKMPREAMQG----LRKLKLLDLTGNHITSVAAEDFEGFNRYLVRLLMASNSLVEIPDEAFGPLSSLTELDLQDNNIRSIRNRAFEGASPTLRHLNLANNLLSSIPFMAFSYLKGLQYLNLERNLISQPYDVMFMGSLSLDTLILDFNAIEYLPPHSFQNFDSVNTSSFRGNPLQKIGADAFKGSKIRELYLQDCDIWNLSEKSFRGIEATLETLDLSYNNLTDIPENIFDRIDSLKWLSLAHNKLILDPKKSFNGFTYTLQYLNMLGE-YMGIIPMDALKNMRNVRTFAFSTFPGFYLSNEDFVGFGPAVEKLYLMNNEISSVKALAF--EHVPGLKVLDLSHNVINSFDNRAFANVGGLEELRVSSGLTLTRLPPQPFHYLINLRILDLSNNKLTFLQNDCLSHMKKLRHLNLEDNKISKLSSHQFRGQFTPELTTIQLSFN---AIPVIESMTFHDLDHLKYIYLDDNMITSVKRFAFANLA-DLEYLSLVGNNIRDLEFEAFQNIPKVRHLDLSYNEMINLNLDAFEQVGTLSSLKIDVSHNKISALKMNGTRWSSISSIKTIDFSYNNISSIDTEYFESVRISLQHLRFSHNNMY-----------------NLSANIF-----SKMLQIQLLDFSN---NHISVVDPEAFRETDLLRSIDLSYNNIKDLPLTLFKNHR-YLSYVDLSGNLMHRMPDDLFRLCPVEILKLSKNNFHGVPDNTLHHITETLRVLDLSWNSLTNISDAVIGGLENLIALDISHNKIHLIGYRSFHDLWRLTSLDVS 911          

HSP 3 Score: 156.762 bits (395), Expect = 3.088e-35
Identity = 192/728 (26.37%), Postives = 326/728 (44.78%), Query Frame = 0
Query:   35 QWISLGLWVGGRGERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIG-QLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLET-QIKLLDLSNCL-IQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNML----------QFSPNIT-----FNGF----RYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVG--NALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKM-TRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGK-LQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQ------------NLH------------RLQRLDLAYNRLESLNFAAFDSIGTLSHL-AIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLK---------------------ILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDL 690
            Q I    + G +     L D  I  LS +  R +  +L  L+L+ N LT +PE IF  +  L+ L L+ N +I     +F      L  L++    MG IP  ++ +++++R    S      +++  F+G    ++ L L  N I ++ A AF++   +    L+ N +   +  AF     ++ L +S+ L +  L       L N L  LDLS N+L  L  D       +R L L DN +          QF+P +T     FN        T  DL+ L     Y+ L++               N   SV    FA+L+  LE L+LV +++  +E  AF+++P +  +DLS N +  +  +AF +VG  ++L+     N +    +    + S+S+++T+D S N+I S+  + F  +   LQHL    N +       F       + +LD S NHI  V+ + FR    L+ + L+ N IK L    F     L  + L GN + ++PD+ F+            N H             L+ LDL++N L +++ A    IG L +L A+D+SHN++  +      ++  L  + SLD+S N ++ +++  F+ +Q+ L+                     +LN S NI+S +  + +  L  IR LD+SHN ++      +     L  L L  N + S+      +  RLQV+D+
Sbjct:  330 QKIGADAFKGSKIRELYLQDCDIWNLSEKSFRGIEATLETLDLSYNNLTDIPENIFDRIDSLKWLSLAHNKLILDPKKSFNGFTYTLQYLNMLGEYMGIIPMDALKNMRNVRTFAFSTFPGFYLSNEDFVGFGPAVEKLYLMNNEISSVKALAFEHVPGLKVLDLSHNVINSFDNRAFANVGGLEELRVSSGLTLTRLPPQPFHYLIN-LRILDLSNNKLTFLQNDCLSHMKKLRHLNLEDNKISKLSSHQFRGQFTPELTTIQLSFNAIPVIESMTFHDLDHLK----YIYLDD---------------NMITSVKRFAFANLA-DLEYLSLVGNNIRDLEFEAFQNIPKVRHLDLSYNEMINLNLDAFEQVGTLSSLKIDVSHNKISALKMNGTRWSSISSIKTIDFSYNNISSIDTEYFESVRISLQHLRFSHNNMYNLSANIFSKML--QIQLLDFSNNHISVVDPEAFRETDLLRSIDLSYNNIKDLPLTLFKNHRYLSYVDLSGNLMHRMPDDLFRLCPVEILKLSKNNFHGVPDNTLHHITETLRVLDLSWNSLTNISDAV---IGGLENLIALDISHNKIHLIGY---RSFHDLWRLTSLDVSNNPLNDIDLYTFDGLQDSLQNLSVANTSIKSFPELQHPKLAVLNASNNILSFLPTNTMANLTGIRTLDVSHNELTAPGSNAWQVMPYLRDLRLQGNFVRSITNSTFASLSRLQVLDI 1028          

HSP 4 Score: 121.709 bits (304), Expect = 2.566e-24
Identity = 149/570 (26.14%), Postives = 251/570 (44.04%), Query Frame = 0
Query:  407 PFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLI-LSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIG-TLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNIS-FVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRN-QKRLQVVDLSHNFLETLPEELFLRT-SLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQ-------------------------LLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTF---GGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELT-----------SPP---------------STAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRA 916
            P  + S + TL L +N +R L   +F+  + L  L ++ N++       F      SL+ LDL +N + ++  +  +   KL+ L L  N I  + ++ F    R L+ L +  N + ++PDE F  L  L  LDL  N + S+   AF+    TL HL  +L++N L ++       +  L  +  L+L  N IS   +V F   +  D  IL+   N I  + P        +       N +  I    F  + K+  LYL    + +L  K  R  +  L+ +DLS+N L  +PE +F R  SL+   +AHN+L   P K+ N    +L+YL++    +  I    L  ++                          +  L L  N+IS++ A AF  VP L+ L+L+ N ++      F   GG+ +      +  T L   P   L +L+ L++S N+LTF+  + L+++ ++R L+L  N+++           +P                S  +H +DHL  ++L  N I  V   +FA L  LE L + G   +  +  A
Sbjct:   93 PVLNSSRVHTLTLRSNKLRQLDERSFYGSSGLWRLEIRHNDLFRIPEKAFLGIGR-SLSELDLRYNELSKMPREAMQGLRKLKLLDLTGNHITSVAAEDFEGFNRYLVRLLMASNSLVEIPDEAFGPLSSLTELDLQDNNIRSIRNRAFEGASPTLRHL--NLANNLLSSIPFM---AFSYLKGLQYLNLERNLISQPYDVMFMGSLSLDTLILDF--NAIEYLPPHSFQNFDSVNTSSFRGNPLQKIGADAFKGS-KIRELYLQDCDIWNLSEKSFRGIEATLETLDLSYNNLTDIPENIFDRIDSLKWLSLAHNKLILDPKKSFNGFTYTLQYLNMLGEYMGIIPMDALKNMRNVRTFAFSTFPGFYLSNEDFVGFGPAVEKLYLMNNEISSVKALAFEHVPGLKVLDLSHNVINSFDNRAFANVGGLEELRVSSGLTLTRLPPQPFHYLINLRILDLSNNKLTFLQNDCLSHMKKLRHLNLEDNKISKLSSHQFRGQFTPELTTIQLSFNAIPVIESMTFHDLDHLKYIYLDDNMITSVKRFAFANLADLEYLSLVGNNIRDLEFEA 653          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Match: gi|1022762791|gb|KZS08454.1| (Chaoptin [Daphnia magna])

HSP 1 Score: 550.821 bits (1418), Expect = 1.994e-175
Identity = 324/812 (39.90%), Postives = 493/812 (60.71%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELT 862
            LG N I E+SRED   +  SL+ L+LA N L ++    F   QRLERLDL  N+I+ I  ++  +G   L+ L LADN +  IPF S+A ++SL  ++L+NNRI    D FF G++ +DTL L  N I NL   AFQNF  IN TSL GN +  I  DAF + +I+ L LS C +  L S + RGLE+SL+ LDLS N L  LPE++   FD ++ L LNDN L F  +   +GFRY ++ +NL+G+ M  +P++++  +RNLR++GLSS++    ++T+ F     +LE L+L  + L +I  N+F+HVP + S+DLS+NRI  IE +AF +VG++L +L M+N +   +LP+ PF+ L +L+++DLSNN I SLP D FH M  ++ + LQDN I        ++   P+L  + LSFN I  +E  TF     L+ L L +N+I ++   +F  +  L  +SL+GN I  +  E F NL +L+ L+L +N LE L+F   D +GTLS + +D+SHN +Q L  NR+  + + S+I SLDL YNNISF+   +FEP+++ L  L L  N +  I+ D    ++ +  LD+S+N I  ++   F  A  L  L L+HN ++ ++  +L     L+V+++SHN L  LP+ LF  T LEI  V+HN++ +IP   L+ + ++L++LD S N+IT+I+  QL ++  LVYL+L  N IST+    F  +  L  L+L+SNP+  +    F  +   L  LN+A+     L +  L  L SLNVS+N +  +P +     + ++E D+S  +LT
Sbjct:  166 LGSNMIVEISREDFSGVEDSLQHLSLADNYLITLQLESFVGFQRLERLDLRGNSILNILPLS-GSGSLKLSHLSLADNALEHIPFTSLAQMRSLNTVNLANNRISTTFDVFFQGRISIDTLVLDNNMIGNLPPFAFQNFNLINKTSLNGNAIREIAEDAFKDAKIRELSLSECSVTVLESKSFRGLESSLQRLDLSYNNLSTLPENLLDKFDFLKALILNDNSLAFKADADLSGFRYALQTINLVGEKMGQIPVKQMNDIRNLRSLGLSSLDE--KISTNDFEGYGAALEHLSLSKNRLKTISSNSFRHVPGLKSLDLSDNRISQIEADAFADVGSSLIHLSMANGIGVGSLPSEPFKKLVSLQSIDLSNNRITSLPDDFFHLMKEIRSINLQDNAIEKIPLQMLNNDHTPNLVNVSLSFNFINAIEAQTFSDLPHLKILNLEENKITRIAKGAFQNIENLEYISLDGNMISTIESEAFHNLPKLETLNLGHNSLEKLSFDWLDQVGTLSAIKLDVSHNLIQQLSSNRTG-WSSYSSIRSLDLGYNNISFISRNYFEPIRSSLTHLVLHHNQLRNISRDVYSDMQHLLWLDISNNNIQLVDSDAFANAKSLQVLLLDHNDISEIYQDMLSRSSTLRVINISHNRLRFLPDTLFKDTQLEILDVSHNQISKIPDGCLSRIATTLRHLDASHNEITSITPDQLKKLTDLVYLDLSNNAISTLTEKTFSSLNRLAYLDLSSNPMQTVADHIFDNLLQSLVHLNLADIGSVDLGDFHLPELLSLNVSYNTVENLPSDFFTRYANLKEFDISYCQLT 973          

HSP 2 Score: 199.134 bits (505), Expect = 1.261e-48
Identity = 244/891 (27.38%), Postives = 412/891 (46.24%), Query Frame = 0
Query:  117 GVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEY-INYTSLAGNPLEWIEPDAF--LETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDN-MLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSL-SALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDN--------EISTFKR--------GTFHSQA-------------------------------NPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVEMTRLLI-LSLE-GNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVV--DLSHNFLETLPEELFLRT------SLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGG---------------------IRD-HLQGLNVANTSLTLLPNLQLS----SLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSF----AGLRHLESLDISGVRAKSFQV 914
            G P + ++   D  +  +P + I + K+   L+L  N + +V +  F  +  L  + +R N++  +   AF   E  +    L  N L+ +   A   LE ++K+LDL + +I E++     G+E+SL+ L L+ N L  L  + F+ F  + RL L  N +L   P ++ +G    +  L+L    + ++P   +  MR+L TV L+  NN  S T D F     S++ L L ++ + ++   AF++   I+   L+ N I  I E+AF++    ++ L +S       L +  FR L S+L+ LDLS N++ +LP +   K   L+ L L DN        ++S F+         G    Q                                  +L  L LS N ++ +  ++FR    L+ L L+DNRI ++++ +F ++   LI LS+  G  +  LP E F+ L  LQ +DL+ NR+ SL    F  +  +   +I+L  N ++ + +   N   T  N++++ LS+N I+ +E   F  + + LKILNL  N I+ I       +  +  + L  N IS IE   F    KL +L L HN L  L    L     L  +  D+SHN ++ L      RT      S+    + +N +  I      P++SSL +L L  NQ+  IS    + +Q L++L++  N I  +D+DAF    +L+ L L  N +  I+ D                          +D  L+ L+V++  ++ +P+  LS    +L+ L+ S N++T I P+ L  L+ +  LDLS N +++     + S++ L  L LS NP+  V +  F      L HL   DI  V    F +
Sbjct:   63 GGPEVGLIYCEDIPLASVP-VGINNTKAF-ALNLRRNGLRRVEENGF-HRTGLWRIDIRNNHLYTVPEMAFAGLERSLGELYLPSNHLQRVPQKALHNLE-KLKVLDLGSNMIVEISREDFSGVEDSLQHLSLADNYLITLQLESFVGFQRLERLDLRGNSILNILP-LSGSG-SLKLSHLSLADNALEHIPFTSLAQMRSLNTVNLA--NNRISTTFDVFFQGRISIDTLVLDNNMIGNLPPFAFQNFNLINKTSLNGNAIREIAEDAFKDA--KIRELSLSEC-SVTVLESKSFRGLESSLQRLDLSYNNLSTLPENLLDKFDFLKALILNDNSLAFKADADLSGFRYALQTINLVGEKMGQIPVKQMNDIRNLRSLGLSSLDEKISTNDFEGYGAALEHLSLSKNRLKTISSNSFRHVPGLKSLDLSDNRISQIEADAFADVGSSLIHLSMANGIGVGSLPSEPFKKLVSLQSIDLSNNRITSLPDDFFHLMKEIR--SINLQDNAIEKIPLQMLNNDHT-PNLVNVSLSFNFINAIEAQTFSDLPH-LKILNLEENKITRIAKGAFQNIENLEYISLDGNMISTIESEAFHNLPKLETLNLGHNSLEKLSFDWLDQVGTLSAIKLDVSHNLIQQLSSN---RTGWSSYSSIRSLDLGYNNISFISRNYFEPIRSSLTHLVLHHNQLRNISRDVYSDMQHLLWLDISNNNIQLVDSDAFANAKSLQVLLLDHNDISEIYQDMLSRSSTLRVINISHNRLRFLPDTLFKDTQLEILDVSHNQISKIPDGCLSRIATTLRHLDASHNEITSITPDQLKKLTDLVYLDLSNNAISTLTEKTFSSLNRLAYLDLSSNPMQTVADHIFDNLLQSLVHLNLADIGSVDLGDFHL 935          

HSP 3 Score: 113.62 bits (283), Expect = 8.457e-22
Identity = 152/637 (23.86%), Postives = 270/637 (42.39%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLA-GNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPF--ISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLE-TQIKLLDLSNCLIQELASNALRGLEN-SLEALDLSANRLQELPED--IFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLT----------------SIEHNAFKHVP-------SISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDN-----------------------EISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQL----------------------LLNDNRIKKLDSQSFVEMTRLLI-------------------------LSLEGNQIRQLPDETFQNLH-RLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLS 586
            L DN+IS++  +    +  SL  L++A G  + S+P   F  L  L+ +DLS N I  +    F   +  +  ++L DN + KIP   ++  H  +L  + LS N I  +    F    HL  L L EN I  +   AFQN E + Y SL GN +  IE +AF    +++ L+L +  +++L+ + L  +   S   LD+S N +Q+L  +   +  + +IR L L  N + F     F   R ++  L L    +  +  +    M++L  + +S+ NN   V +D FA+ + SL+ L L  +D++                +I HN  + +P        +  +D+S+N+I  I +     +   L++L  S+     ++     + L+ L  LDLSNN I +L   TF  + RL +L L  N                       +I +   G FH    P L  L++S+N +E +  D F R+  L++                       +    ++K LD    ++    +                          +++ G +++ +    F++   +   L L  N +  L  A F ++G +  L ++L HN+L  +    +  +P  S
Sbjct:  480 LSDNRISQIEADAFADVGSSLIHLSMANGIGVGSLPSEPFKKLVSLQSIDLSNNRITSLPDDFFHL-MKEIRSINLQDNAIEKIPLQMLNNDHTPNLVNVSLSFNFINAIEAQTFSDLPHLKILNLEENKITRIAKGAFQNIENLEYISLDGNMISTIESEAFHNLPKLETLNLGHNSLEKLSFDWLDQVGTLSAIKLDVSHNLIQQLSSNRTGWSSYSSIRSLDLGYNNISFISRNYFEPIRSSLTHLVLHHNQLRNISRDVYSDMQHLLWLDISN-NNIQLVDSDAFAN-AKSLQVLLLDHNDISEIYQDMLSRSSTLRVINISHNRLRFLPDTLFKDTQLEILDVSHNQISKIPDGCLSRIATTLRHLDASHN-EITSITPDQLKKLTDLVYLDLSNNAISTLTEKTFSSLNRLAYLDLSSNPMQTVADHIFDNLLQSLVHLNLADIGSVDLGDFHL---PELLSLNVSYNTVENLPSDFFTRYANLKEFDISYCQLTEGSLFQMTNLRHLAIGTYEKVKGLDIPKLLDFNHAIKHLEIDVETATLDSQLKGKLPFKIGNITISGKKLKSIASNAFKDFQGKALTLTLQDNGVTELTKALFQNLGNVRWLQLELRHNKLSTVAEPSTTIHPGTS 1109          

HSP 4 Score: 74.7146 bits (182), Expect = 8.787e-10
Identity = 107/479 (22.34%), Postives = 213/479 (44.47%), Query Frame = 0
Query:  441 LYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLI-LSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSI-GTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNI-SFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRA 916
            +Y +D  +++   G  +++A      L+L  N + RVE + F    L ++ + +N +  +   +F  + R L  L L  N ++++P +   NL +L+ LDL  N +  ++   F  +  +L HL+  L+ N L  L++    ++     +  LDL  N+I + + ++    ++  L  L+L+ N +  I    + Q+R +  ++L++N IS   D  F     + +L L++N + +L     +N   +    L+ N +  + E+ F    +    ++   +  +  K+   ++SSL+ LDLS N ++T+ ++ L++   L  L L  N ++   ADA                          G R  LQ +N+    +  +P  Q++ +++L                 LS + E  +S N+     +   H       L LS N +  + ++SF  +  L+SLD+S  R    +  A
Sbjct:   70 IYCEDIPLASVPVGINNTKA----FALNLRRNGLRRVEENGFHRTGLWRIDIRNNHLYTVPEMAFAGLERSLGELYLPSNHLQRVPQKALHNLEKLKVLDLGSNMIVEISREDFSGVEDSLQHLS--LADNYLITLQLE---SFVGFQRLERLDLRGNSILNILPLSGSGSLK--LSHLSLADNALEHIPFTSLAQMRSLNTVNLANNRISTTFDVFFQGRISIDTLVLDNNMIGNLPPFAFQNFNLINKTSLNGNAIREIAEDAFKDAKIRELSLSECSVTVLESKSFRGLESSLQRLDLSYNNLSTLPENLLDKFDFLKALILNDNSLA-FKADA-----------------------DLSGFRYALQTINLVGEKMGQIPVKQMNDIRNLRS-------------LGLSSLDE-KISTNDFEGYGAALEH-------LSLSKNRLKTISSNSFRHVPGLKSLDLSDNRISQIEADA 492          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Match: gi|970902791|ref|XP_015117217.1| (PREDICTED: chaoptin [Diachasma alloeum])

HSP 1 Score: 531.946 bits (1369), Expect = 2.014e-167
Identity = 322/866 (37.18%), Postives = 514/866 (59.35%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKV----ADPFFIG-QLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQ-FSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTL-----------SNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKAL-NPVQSSLKYLDLSANQITTI-SDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNP--LHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAG 895
            L  NQIS ++ ++ R L  SL+ L L  N +  +P   F  L+ LE LDL  NN+++  S  F+ G+ HL  L L+ N +  IP+  I+ +K L++LDLS N+I ++     +P   G Q+ LDTL+L  N I+ L   +FQ+F  +N T L+GNP+  +E  AF +++I+ L  ++C I+E+ S +LRGLE+SLE LDLS N + +LP  +F +FD +R L   +N +  FSP   F GF+Y++ +L L G++   V L+++  MRNLR + +S +    +++ D F +    ++ L+++ S+L +I+ + F HV  I  +D S N I  IEENAF EVG++L  L++++AL    +P  PF +L+ L+ LDLSNN +RS+P ++FH + R++ + +QDNEIS  K+GTF    + +L  ++  +N I  ++  TF     +  L L DN+I+K++ ++F+ M RL  L+L GN+++ + DE+FQNL  L+ LD+AYN+L   +F  FD +GTL+ L ++ SHN +  L++N S+  P             SNI  LDLS+NNI+ +   +F PV+  L  L LS N +  +T    G L  ++ LDL HN I  IE   F     +  L L+HN +  +   +LR  K+L+++DLSHN L TLP+ +F   +LE   ++HN+   +P+K+L  P   SL  LD+  N ++ I +   +++++ L +L++  N++  +D   F  +P+L +L+L+ N   L      TF G+ D L  L ++N SL  +P L    L++L ++ N+L  IP    +NLS +  LDLS N+LT  P    H++  L S +L+ NPI  V N SF G
Sbjct:  153 LTGNQISSIAADNWRGLENSLQTLRLGKNTIDKLPADAFSGLKFLETLDLRENNLMEFDSSVFRDGMAHLNYLYLSGNQLTMIPYSQISPLKHLKILDLSYNQIRRMLQTQTEPEIQGLQISLDTLRLDWNQIETLPVASFQHFFKVNKTYLSGNPIGHVEEGAFKDSKIRELYFTDCDIREIDSASLRGLESSLEHLDLSGNNITDLPNHVFQEFDFLRTLIFRENKITTFSPQEVFTGFQYSLYNLELSGRENAAVSLQDLRQMRNLRFLSISKMPQ-STLSPDDFLEFGIDVKELHIIQSNLNTIKGHTFTHVRGIKYLDFSENSISSIEENAFTEVGHSLITLKLAHALSMTEVPAKPFAALTNLQYLDLSNNQLRSMPENSFHFLKRIKRIEIQDNEISDIKKGTFQGDIHSTLEEVNFGYNQISNLQTHTFVDLAAMTILNLQDNKIEKIERRAFMNMNRLKYLNLRGNKMKTINDESFQNLPDLEFLDMAYNQLPEFDFEWFDQVGTLAALKVNASHNGITRLKINSSSYLPATSMYFAVGGTLQSNIKVLDLSWNNITDIMKFYFRPVEYSLTHLYLSHNELMNMTQGVFGSLPHLQWLDLRHNDIMEIEFDCFRNTKNIQVLLLSHNDIVDIPADVLRTLKKLRILDLSHNRLRTLPDNVFTEPNLESLDLSHNQFMRLPIKSLAGPAAGSLSVLDMGWNMLSGIHTTDSISRLKGLTHLDVSYNRLVRLDDGVFSELPHLTELDLSHNKQLLLESRGRTFYGLEDSLLTLGLSNISLLSVPELPFHRLRTLYLANNELASIPAELASNLSSLHHLDLSHNDLTVVPLIT-HTLSELRSFNLAYNPITAVTNTSFLG 1016          

HSP 2 Score: 179.489 bits (454), Expect = 2.471e-42
Identity = 221/839 (26.34%), Postives = 379/839 (45.17%), Query Frame = 0
Query:  117 GVPHLAVLDLADNLMGKIPFISIAHVKSLRV--LDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYT-SLAGNPLEWIEPDAFLETQ-IKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLND-NMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSV-------TTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSL-SALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFR-FGKLQQLLLNDNRIKKLDSQSFVE----MTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIG------TLSHL------------------AIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEV-TFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRT-SLEIFKV--AHNRLYE--------IPVKAL-----NPVQSSLKYLDLSANQITTISDSQLNQIQL-LVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAG 895
             +P L +++  +  M +IP      + S +V  L L NN +  +  P F+    L  LQ+R N + ++   AF   E   +   L  N L  +   +F   Q +K+LDL+   I  +A++  RGLENSL+ L L  N + +LP D F     +  L L + N+++F  ++  +G  + +  L L G  +  +P  +I  +++L+ + LS    Y  +       T  +   L  SL+ L L  + + ++   +F+H   ++   LS N I ++EE AF++  + ++ L  ++    R + +   R L S+L  LDLS N+I  LP   F +   L+ L  ++N+I+TF      +    SL  L+LS      V     R    L+ L ++      L    F+E    +  L I+    N I+     TF ++  ++ LD + N + S+   AF  +G       L+H                    +DLS+NQL+++     N++  L  I  +++  N IS ++  TF   + + L+ +N   N IS +       L  + +L+L  N I  IE   F   N+L  L L  N++ +++ +  +N   L+ +D+++N L     E F +  +L   KV  +HN +          +P  ++       +QS++K LDLS N IT I       ++  L +L L  N++  +    F  +P+L+ L+L  N +  I  D F          N  N  + LL             S N +  IP + L  L ++R LDLS N L + P   + +  +L SL LS N  +R+   S AG
Sbjct:   50 AIPDLGIVNCENVPMPRIP----QQINSSKVFILKLGNNGLRHI-QPQFLVTTGLYNLQIRNNPLSDIPDEAFLGLERSLWELELPYNQLVKVPSKSFRHLQKLKILDLTGNQISSIAADNWRGLENSLQTLRLGKNTIDKLPADAFSGLKFLETLDLRENNLMEFDSSVFRDGMAH-LNYLYLSGNQLTMIPYSQISPLKHLKILDLS----YNQIRRMLQTQTEPEIQGLQISLDTLRLDWNQIETLPVASFQHFFKVNKTYLSGNPIGHVEEGAFKD--SKIRELYFTDC-DIREIDSASLRGLESSLEHLDLSGNNITDLPNHVFQEFDFLRTLIFRENKITTFSPQEVFTGFQYSLYNLELSGRENAAVSLQDLRQMRNLRFLSISKMPQSTLSPDDFLEFGIDVKELHIIQSNLNTIK---GHTFTHVRGIKYLDFSENSISSIEENAFTEVGHSLITLKLAHALSMTEVPAKPFAALTNLQYLDLSNNQLRSM---PENSFHFLKRIKRIEIQDNEISDIKKGTFQGDIHSTLEEVNFGYNQISNLQTHTFVDLAAMTILNLQDNKIEKIERRAFMNMNRLKYLNLRGNKMKTINDESFQNLPDLEFLDMAYNQLPEFDFEWFDQVGTLAALKVNASHNGITRLKINSSSYLPATSMYFAVGGTLQSNIKVLDLSWNNITDIMKFYFRPVEYSLTHLYLSHNELMNMTQGVFGSLPHLQWLDLRHNDIMEIEFDCFR---------NTKNIQVLLL-------------SHNDIVDIPADVLRTLKKLRILDLSHNRLRTLPDNVF-TEPNLESLDLSHNQFMRLPIKSLAG 846          

HSP 3 Score: 162.925 bits (411), Expect = 3.634e-37
Identity = 204/798 (25.56%), Postives = 357/798 (44.74%), Query Frame = 0
Query:   45 GRGERCALGDNQISELSRED----------LRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPH-LAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVA-DPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKL--LDLSNCL-IQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNG-FRYTIKDLNLLG------QDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVG-----------NALQYLRMSNALHFRALP-----NGPFRSLSALRTLDLSNNHIRSLPLDTFHKMT-RLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGK-LQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRN---IISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVP--NLEQLNLASNPLHRIHLD 797
            G  E  A  D++I EL   D          LR L  SL  L+L+GN +T +P  +F +   L  L    N I         TG  + L  L+L+      +    +  +++LR L +S      ++ D F    + +  L + ++N+  +  + F +   I Y   + N +  IE +AF E    L  L L++ L + E+ +     L N L+ LDLS N+L+ +PE+ F     I+R+ + DN +      TF G    T++++N         Q   +V L  + I+ NL+       N    +    F +++  L+ LNL  + + +I   +F+++P +  +D++ N++   +   F +VG           N +  L+++++ +  A        G  +S   ++ LDLS N+I  +    F  +   L HLYL  NE+    +G F S   P L  LDL  N I  +E+D FR  K +Q LLL+ N I  + +     + +L IL L  N++R LPD  F   + L+ LDL++N+   L   +       S   +D+  N L    ++ +++   L  +  LD+SYN +  ++   F  + + L  L+LS N   ++        G    +  L LS+  +  + +  F   ++L +LYL +N L S+  +L  N   L  +DLSHN L  +P      + L  F +A+N +  +   +      SL  LD+    + T     L           + +K+ ++   A+  +   NL  L   ++ L  +H+D
Sbjct:  310 GHVEEGAFKDSKIRELYFTDCDIREIDSASLRGLESSLEHLDLSGNNITDLPNHVFQEFDFLRTLIFRENKITTFSPQEVFTGFQYSLYNLELSGRENAAVSLQDLRQMRNLRFLSISKMPQSTLSPDDFLEFGIDVKELHIIQSNLNTIKGHTFTHVRGIKYLDFSENSISSIEENAFTEVGHSLITLKLAHALSMTEVPAKPFAALTN-LQYLDLSNNQLRSMPENSFHFLKRIKRIEIQDNEISDIKKGTFQGDIHSTLEEVNFGYNQISNLQTHTFVDLAAMTIL-NLQD------NKIEKIERRAFMNMN-RLKYLNLRGNKMKTINDESFQNLPDLEFLDMAYNQLPEFDFEWFDQVGTLAALKVNASHNGITRLKINSSSYLPATSMYFAVGGTLQS--NIKVLDLSWNNITDIMKFYFRPVEYSLTHLYLSHNELMNMTQGVFGSL--PHLQWLDLRHNDIMEIEFDCFRNTKNIQVLLLSHNDIVDIPADVLRTLKKLRILDLSHNRLRTLPDNVFTEPN-LESLDLSHNQFMRLPIKSLAGPAAGSLSVLDMGWNMLSG--IHTTDSISRLKGLTHLDVSYNRLVRLDDGVFSELPH-LTELDLSHNKQLLLESRGRTFYGLEDSLLTLGLSNISLLSVPELPF---HRLRTLYLANNELASIPAELASNLSSLHHLDLSHNDLTVVPLITHTLSELRSFNLAYNPITAVTNTSFLGPADSLIELDMRRLPLWTFESGAL----------CKASKLRSLHVTAYTAIKNFNLPSLLEHNHGLRHLHID 1077          

HSP 4 Score: 136.346 bits (342), Expect = 8.858e-29
Identity = 187/731 (25.58%), Postives = 323/731 (44.19%), Query Frame = 0
Query:  268 LRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRS-LPLDTFHKMTRLQ----HLYLQDNEISTFKRGTFHS--QANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEM-TRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLN----FAAFDSIGTLSHLAIDLSHN---QLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFG-AANKLSSLYLNHN-RLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELF-LRTSLEIFKVAHNRLYEIPVKAL-NPVQSSLK----------------YLDLSANQITTISDSQLNQIQL--------LVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANT----------SLTLLPNLQL---------SSLKSLNVSFNQLT-----------------FIPPNTLANLSQ--------VRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKS 911
            L++ +N L   P+  F G   ++ +L L    +  VP +    ++ L+ + L+  N   S+  D +  L  SL+ L L  + +  +  +AF  +  + ++DL  N +   + + FR+    L YL +S       +P      L  L+ LDLS N IR  L   T  ++  LQ     L L  N+I T    +F    + N +     LS N I  VE   F+  K+++L   D  I+++DS S   + + L  L L GN I  LP+  FQ    L+ L    N++ + +    F  F    +L +L +    N    LQ LR  R+  + ++S +    LS ++  F+E         D+K L++ ++ ++ I       +R I+ LD S N IS IE+  F    + L +L L H   +T +  K       LQ +DLS+N L ++PE  F     ++  ++  N + +I        + S+L+                ++DL+A  I  + D+++ +I+         L YLNL GNK+ TI+ ++F  +P+LE L++A N L     + F  +   L  L V  +          S + LP   +         S++K L++S+N +T                 ++  N L N++Q        ++ LDL  N++       + +  ++  L LS N IV +  D    L+ L  LD+S  R ++
Sbjct:  102 LQIRNNPLSDIPDEAFLGLERSLWELELPYNQLVKVPSKSFRHLQKLKILDLTG-NQISSIAADNWRGLENSLQTLRLGKNTIDKLPADAFSGLKFLETLDLRENNLMEFDSSVFRDGMAHLNYLYLSGN-QLTMIPYSQISPLKHLKILDLSYNQIRRMLQTQTEPEIQGLQISLDTLRLDWNQIETLPVASFQHFFKVNKTY----LSGNPIGHVEEGAFKDSKIRELYFTDCDIREIDSASLRGLESSLEHLDLSGNNITDLPNHVFQEFDFLRTLIFRENKITTFSPQEVFTGFQY--SLYNLELSGRENAAVSLQDLRQMRNLRFLSISKMPQSTLSPDD--FLEFGI------DVKELHIIQSNLNTIKGHTFTHVRGIKYLDFSENSISSIEENAFTEVGHSLITLKLAHALSMTEVPAKPFAALTNLQYLDLSNNQLRSMPENSFHFLKRIKRIEIQDNEISDIKKGTFQGDIHSTLEEVNFGYNQISNLQTHTFVDLAAMTILNLQDNKIEKIERRAFMNMNRLKYLNLRGNKMKTINDESFQNLPDLEFLDMAYNQLPEFDFEWFDQV-GTLAALKVNASHNGITRLKINSSSYLPATSMYFAVGGTLQSNIKVLDLSWNNITDIMKFYFRPVEYSLTHLYLSHNELMNMTQGVFGSLPHLQWLDLRHNDIMEIEFDCFRNTKNIQVLLLSHNDIVDIPADVLRTLKKLRILDLSHNRLRT 815          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Match: gi|1068414380|ref|XP_018053036.1| (PREDICTED: chaoptin [Atta colombica])

HSP 1 Score: 515.768 bits (1327), Expect = 2.676e-161
Identity = 332/890 (37.30%), Postives = 519/890 (58.31%), Query Frame = 0
Query:   48 ERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKV----ADPFFIG-QLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQ-FSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALH--FRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPT-------LSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSS-LKYLDLSANQITTI-SDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNP--LHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLR-HLESLDISGVRAKSFQVRA 916
            E   L  N+IS +  E+ R L  SL+ L L  NA+  +P   F  L  LE LDL  N++ +I    F+ G+ HL  L L DN +  +P+I +  +K ++VLDLS N+I K+     +P     Q+ LD LQL  N I+ L +  FQ+F  +N+T L GNPL  IE   F +++I+ L LS+C + E+ S  L GLE+SLE LD+S N +  LP  +F +FD +R L   +N +  FSP   F+GF+Y + +L+L G+    + L+++  MRNLR + +S +     ++ + F +    ++ L +++S+L +I+++AF HV  I  +D S N I  I+++AF EVG +L  LRMS+ L      +PN PF+SL+ L+ LDLSNN IRSL   +FH + R++ + LQDNEI +  +GTF    + +L  ++ +FN +  ++  TF     L  + L DN I++++ ++F+ M RL  ++L GN+IR + DE FQNL  L+ LDLAYN L   +FA+FD +GTLS   +++SHN++  L VN ++   +        SNI  LDLSYNNIS +   +F+PV+  L  L LS N +  IT D  G +  ++ LDL HN +  I        N L  L+ + N +T +  + LR+ K+L++VDLSHN L TLP+ +F  + +E   ++HN+   +PVK+++   ++ L  LD+S N ++ I +   + +++ LV+L+L  N++  +D   F  +P L  L+L+ N   +      TF G+ D L  L ++N SL  +P L L  L++LN++ N L  I P   +NL+ +  LDLS N+LT  P    H++  L + +L+ NPI  + N SF G+   LE LDI  +   SF+  A
Sbjct:  149 EVLELTGNKISRIVPENWRGLENSLQILRLGKNAIEKLPADAFVSLTYLEILDLRENSLKEIDPSVFRDGMAHLTHLYLNDNQLTYVPYIQLFSLKRMKVLDLSYNKISKMLHVQQEPEIRSIQMSLDVLQLDYNQIETLASGDFQHFYKVNHTYLRGNPLMTIEEGTFKDSRIRELYLSDCDLLEINSANLVGLESSLELLDVSGNNITMLPNHLFQEFDLLRTLIFRENRINTFSPTEVFSGFQY-LYNLDLSGKQNSMISLQDLRQMRNLRFLSISRMPQ-AILSANDFLEFGMDIKELRIINSNLNTIKNHAFMHVRGIKYLDFSENSISTIDDDAFSEVGYSLLTLRMSHGLSSSISEIPNRPFKSLTNLQHLDLSNNKIRSLSATSFHFLKRIKRIELQDNEIDSILKGTFQGDIHSTLEEINFAFNQVRDLQTHTFIDLSALMTINLEDNTIERIEKRAFINMNRLKYINLRGNKIRDITDEAFQNLPDLEFLDLAYNNLNEFDFASFDQVGTLSSFKVNVSHNEISRLWVNSTSFISSSGVSGNVQSNIKVLDLSYNNISDITKYYFKPVEYSLTHLYLSNNQLRNITQDVFGNMPHLQWLDLRHNGLIEIYFDCLKNTNNLQVLFFSWNEITDIPAETLRSLKKLRIVDLSHNKLRTLPDNMFTDSDIESLDLSHNQFTRLPVKSMSVTSTANLANLDMSWNILSGIHTTDTIFRLRGLVWLDLSYNRLVRLDDGVFSDLPYLAHLDLSHNKQLILESRGRTFYGLEDTLLYLGLSNISLLSIPELPLRRLQTLNLAHNGLASISPEMSSNLTSLHYLDLSYNDLTVVPLIT-HTLPELKTFNLANNPITAITNTSFLGVADSLEELDIRKLSLSSFEAGA 1035          

HSP 2 Score: 161.77 bits (408), Expect = 9.845e-37
Identity = 221/872 (25.34%), Postives = 392/872 (44.95%), Query Frame = 0
Query:  117 GVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEY-INYTSLAGNPLEWIEPDAFLETQ-IKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNML-QFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSP-------SLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHS------------QANPSLAILD------LSFNHIERVEY------DTFRFG-KLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNL-HRLQRLDLAYNRLESLNFA---AFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNI-SFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRT-SLEIFK--VAHNRLYEIPVKALN---------PVQSSLKYLDLSANQITTISDSQLNQIQL-LVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNV---SFNQLTFIPPNTLANLSQVRELDLSGNELTSPP-------STA--------W------HSMD------HLLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
             +P L  +      M +IP     +   + +L L NN + +V  P ++    L  LQ++ N + ++   AF   E  ++   L+ N L  I   +F   Q +++L+L+   I  +     RGLENSL+ L L  N +++LP D F+    +  L L +N L +  P++  +G  + +  L L    + YVP  ++  ++ ++ + LS    Y  ++        P       SL+ L L  + + ++    F+H   ++   L  N +  IEE  F++      YL   + L   +         S+L  LD+S N+I  LP   F +   L+ L  ++N I+TF      S            + N  +++ D      L F  I R+        D   FG  +++L + ++ +  + + +F+ +  +  L    N I  + D+ F  + + L  L +++    S++      F S+  L HL  DLS+N++++L    + ++  L  I  ++L  N I S ++ TF   + + L+ +N + N + ++       L  +  ++L  N I  IE   F   N+L  + L  N++  +  +  +N   L+ +DL++N L       F +  +L  FK  V+HN +  + V + +          VQS++K LDLS N I+ I+      ++  L +L L  N++  I  D F  +P+L+ L+L  N L  I+ D      ++LQ L  +   +T +P   L SLK L +   S N+L  +P N   + S +  LDLS N+ T  P       STA        W      H+ D       L+ L LS N +VR+ +  F+ L +L  LD+S
Sbjct:   50 AMPDLGKVACYSVPMPRIP--QPINSSKMYMLQLENNGL-RVLQPQYLMNTGLYQLQIKHNPLSDIPEEAFLGLERSLSMLDLSYNQLASIPSKSFKHLQKLEVLELTGNKISRIVPENWRGLENSLQILRLGKNAIEKLPADAFVSLTYLEILDLRENSLKEIDPSVFRDGMAH-LTHLYLNDNQLTYVPYIQLFSLKRMKVLDLS----YNKISKMLHVQQEPEIRSIQMSLDVLQLDYNQIETLASGDFQHFYKVNHTYLRGNPLMTIEEGTFKDSRIRELYLSDCDLLEINSANLVGLE--SSLELLDVSGNNITMLPNHLFQEFDLLRTLIFRENRINTFSPTEVFSGFQYLYNLDLSGKQNSMISLQDLRQMRNLRFLSISRMPQAILSANDFLEFGMDIKELRIINSNLNTIKNHAFMHVRGIKYLDFSENSISTIDDDAFSEVGYSLLTLRMSHGLSSSISEIPNRPFKSLTNLQHL--DLSNNKIRSL---SATSFHFLKRIKRIELQDNEIDSILKGTFQGDIHSTLEEINFAFNQVRDLQTHTFIDLSALMTINLEDNTIERIEKRAFINMNRLKYINLRGNKIRDITDEAFQNLPDLEFLDLAYNNLNEFDFASFDQVGTLSSFKVNVSHNEISRLWVNSTSFISSSGVSGNVQSNIKVLDLSYNNISDITKYYFKPVEYSLTHLYLSNNQLRNITQDVFGNMPHLQWLDLRHNGLIEIYFDCLKNT-NNLQVLFFSWNEITDIPAETLRSLKKLRIVDLSHNKLRTLPDNMFTD-SDIESLDLSHNQFTRLPVKSMSVTSTANLANLDMSWNILSGIHTTDTIFRLRGLVWLDLSYNRLVRLDDGVFSDLPYLAHLDLS 904          

HSP 3 Score: 102.064 bits (253), Expect = 3.197e-18
Identity = 143/558 (25.63%), Postives = 251/558 (44.98%), Query Frame = 0
Query:  403 LPNGPFRSLS-ALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVE-MTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAID---LSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDD-VGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTS-----------------LHGKL--------LRNQKRLQVVDLSHNFLETLPEELFLRTSLEI--FKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQL-LVYLNLE---GNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGI----RDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRASD 918
            +P   F  L  +L  LDLS N + S+P  +F  + +L+ L L  N+IS      +    N SL IL L  N IE++  D F     L+ L L +N +K++D   F + M  L  L L  NQ+  +P     +L R++ LDL+YN++  +     +       +++D   L +NQ++ L    S  +     +    L  N +  +E   F+   + ++ L LS   + EI   + VG    + +LD+S N I+ + +  F   + L +L    NR+ +                 L GK         LR  + L+ + +S      L    FL   ++I   ++ ++ L  I   A   V+  +KYLD S N I+TI D   +++   L+ L +     + IS I    F  + NL+ L+L++N +  +   +F  +    R  LQ   + +           S+L+ +N +FNQ+  +  +T  +LS +  ++L  N +      A+ +M+ L  ++L GN I  + +++F  L  LE LD++      F   + D
Sbjct:  112 IPEEAFLGLERSLSMLDLSYNQLASIPSKSFKHLQKLEVLELTGNKISRIVPENWRGLEN-SLQILRLGKNAIEKLPADAFVSLTYLEILDLRENSLKEIDPSVFRDGMAHLTHLYLNDNQLTYVPYIQLFSLKRMKVLDLSYNKISKMLHVQQEPEIRSIQMSLDVLQLDYNQIETLA---SGDFQHFYKVNHTYLRGNPLMTIEEGTFK--DSRIRELYLSDCDLLEINSANLVGLESSLELLDVSGNNITMLPNHLFQEFDLLRTLIFRENRINTFSPTEVFSGFQYLYNLDLSGKQNSMISLQDLRQMRNLRFLSISRMPQAILSANDFLEFGMDIKELRIINSNLNTIKNHAFMHVRG-IKYLDFSENSISTIDDDAFSEVGYSLLTLRMSHGLSSSISEIPNRPFKSLTNLQHLDLSNNKIRSLSATSFHFLKRIKRIELQDNEIDSILKGTFQGDIHSTLEEINFAFNQVRDLQTHTFIDLSALMTINLEDNTIERIEKRAFINMNRLKYINLRGNKIRDITDEAFQNLPDLEFLDLAYNNLNEFDFASFD 662          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Match: gi|780685750|ref|XP_011698502.1| (PREDICTED: chaoptin isoform X3 [Wasmannia auropunctata])

HSP 1 Score: 515.383 bits (1326), Expect = 3.511e-161
Identity = 330/886 (37.25%), Postives = 517/886 (58.35%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKV----ADPFFIG-QLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNML-QFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALH--FRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSN-TYPTL------SNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQS-SLKYLDLSANQITTI-SDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNP--LHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLR-HLESLDISGVRAKSFQVRA 916
            L  N+IS +  E+ R L  SL+ L L  NA+  +P   F  L  LE LDL  N++ +I    F+ G+ HL  L L DN +  +P+  ++ +K ++VLDLS NRI K+     +P   G Q+ LD LQL  N I+NL +  FQ+F  +N T L GNPL  IE   F  ++I+ L LS+C + E+ S  L GLE SLE LD+S N +  LP  +F +FD +R L   +N +  F P   FNGF+Y++ +L+L G+    + L+++  MRN+R++ +S +    +++ + F +    ++ L +V+S+L +I+ +AF HV  I  +D S N I  I++ AF EVG++L  LRMS+ L      +PN PF+SL+ L+ LD SNN IRSLP  +FH + R++ +  QDNEI    +GTF    + ++  ++ +FN ++ ++  TF     L  + L DN I+K++ ++F+ M RL  ++L GN+I+ + DE FQNL  L+ LDLAYN L   +FA+FD +GTLS   +++SHN++  L VN +  T P +      SNI  LDLSYNNIS +   +F+PV+  L  L LS N +  IT D    +  ++ LD+ HN ++  +   F     L  L  + N +T +  + LR  K+L++VDLSHN L TLP+ +F  +++E   ++HN+   +PVK+++ + + SL  LD+S N ++ I S   + +++ LV+L+L  N++  +D   F  +  L  L+L+ N   +      TF G+ D L  L ++N SL  +P L L  L++L ++ N+L  IPP   +NL+ +  LDLS N+LT  P    H++  L + +L+ NPI  + N SF G+   LE LDI  +    F+  A
Sbjct:  148 LTGNKISRIVPENWRGLENSLQTLRLGRNAIEKLPADAFVGLTYLEILDLRENSLKEIDPSVFRDGMAHLTHLYLNDNQLTYVPYAQLSLLKRMKVLDLSYNRISKMLQTQQEPEIRGIQMSLDVLQLDYNQIENLASGDFQHFFKVNRTYLRGNPLMIIEEGTFRNSRIRELYLSDCDLLEINSANLAGLELSLELLDVSGNNITMLPNGLFQEFDFLRTLVFRENRIGTFLPTEVFNGFQYSLYNLDLSGKQNSMISLQDLRQMRNMRSLSISRMPQ-TTLSPNDFLEFGMDIKELRIVNSNLNTIKGHAFMHVRGIKYLDFSENSISTIDDEAFSEVGHSLLTLRMSHGLSSTISEMPNRPFKSLTNLQHLDFSNNKIRSLPATSFHFLKRIKRIEFQDNEIDNIPKGTFQGDIHSTMEEINFAFNQVKNLQTHTFVDLSALMAINLEDNVIEKIERRTFMNMNRLKYINLRGNKIKDITDEAFQNLPDLEFLDLAYNNLNEFDFASFDQVGTLSSFKVNVSHNEISRLWVNNTVFTSPFVVGGNVQSNIKVLDLSYNNISEITKYYFKPVEYSLTHLYLSNNKLKNITQDIFDNMPHLQWLDMRHNVLTEPDFDCFKNTKNLQVLLFSWNEITDIPAETLRPLKKLRIVDLSHNKLRTLPDNMFTDSNVESLDLSHNQFTRLPVKSMSVISTASLANLDMSWNILSGIHSADTIFRLRGLVWLDLSYNRLVRLDDGVFSDLSYLAHLDLSHNKQLILESRGRTFYGLEDTLLYLGLSNISLLSVPELPLRRLQALYLAHNELASIPPEMSSNLTSLHYLDLSFNDLTVVPLIT-HTLPELKTFNLADNPITAITNTSFLGIADSLEELDIRRLTLSVFEAGA 1031          

HSP 2 Score: 168.318 bits (425), Expect = 7.274e-39
Identity = 223/869 (25.66%), Postives = 383/869 (44.07%), Query Frame = 0
Query:  117 GVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYT-SLAGNPLEWIEPDAFLETQ-IKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNML-QFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNA---FKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNH-------------------IERVEY------DTFRFG-KLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNL-HRLQRLDLAY---NRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFV-EVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRT-SLEIFKV-----------AHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQL-LVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNV---SFNQLTFIPPNTLANLSQVRELDLSGNELTSPP-------STA--------W------HSMD------HLLSLHLSGNPIVRVMNDSFAGLRHLESLDIS 905
             +P L ++   +  M +IP     +   + +L L NN + +   P ++    L  LQ++ N + ++   AF   E   +   L+ N L  +   +F   Q ++LL+L+   I  +     RGLENSL+ L L  N +++LP D F+    +  L L +N L +  P++  +G  + +  L L    + YVP  ++ +++ ++ + LS       + T Q  ++     +L+++  D   IE+ A   F+H   ++   L  N +  IEE  FR       YL   + L   +  N     LS L  LD+S N+I  LP   F +   L+ L  ++N I TF      +    SL  LDLS                      I R+        D   FG  +++L + ++ +  +   +F+ +  +  L    N I  + DE F  + H L  L +++   + +  +    F S+  L HL  D S+N++++L    + ++  L  I  ++   N I  + + TF   + + ++ +N + N +  +       L  +  ++L  N I  IE  TF   N+L  + L  N++  +  +  +N   L+ +DL++N L       F +  +L  FKV            +N ++  P      VQS++K LDLS N I+ I+      ++  L +L L  NK+  I  D F  +P+L+ L++  N L     D F   ++ LQ L  +   +T +P   L  LK L +   S N+L  +P N   + S V  LDLS N+ T  P       STA        W      HS D       L+ L LS N +VR+ +  F+ L +L  LD+S
Sbjct:   45 AIPDLGIVACYNVPMPRIP--QPINSSKVFMLQLENNGL-RFLQPQYLMNTGLYKLQIKHNPLADIPEEAFLGLERSLWELDLSYNQLASVPSKSFRHLQKLRLLELTGNKISRIVPENWRGLENSLQTLRLGRNAIEKLPADAFVGLTYLEILDLRENSLKEIDPSVFRDGMAH-LTHLYLNDNQLTYVPYAQLSLLKRMKVLDLSYNRISKMLQTQQEPEIRGIQMSLDVLQLDYNQIENLASGDFQHFFKVNRTYLRGNPLMIIEEGTFRNSRIRELYLSDCDLLEINS-ANLAGLELS-LELLDVSGNNITMLPNGLFQEFDFLRTLVFRENRIGTFLPTEVFNGFQYSLYNLDLSGKQNSMISLQDLRQMRNMRSLSISRMPQTTLSPNDFLEFGMDIKELRIVNSNLNTIKGHAFMHVRGIKYLDFSENSISTIDDEAFSEVGHSLLTLRMSHGLSSTISEMPNRPFKSLTNLQHL--DFSNNKIRSL---PATSFHFLKRIKRIEFQDNEIDNIPKGTFQGDIHSTMEEINFAFNQVKNLQTHTFVDLSALMAINLEDNVIEKIERRTFMNMNRLKYINLRGNKIKDITDEAFQNLPDLEFLDLAYNNLNEFDFASFDQVGTLSSFKVNVSHNEISRLWVNNTVFTSPFVVGGNVQSNIKVLDLSYNNISEITKYYFKPVEYSLTHLYLSNNKLKNITQDIFDNMPHLQWLDMRHNVLTEPDFDCFKNTKN-LQVLLFSWNEITDIPAETLRPLKKLRIVDLSHNKLRTLPDNMFTD-SNVESLDLSHNQFTRLPVKSMSVISTASLANLDMSWNILSGIHSADTIFRLRGLVWLDLSYNRLVRLDDGVFSDLSYLAHLDLS 900          

HSP 3 Score: 162.54 bits (410), Expect = 6.038e-37
Identity = 200/796 (25.13%), Postives = 355/796 (44.60%), Query Frame = 0
Query:   37 ISLGLWVGGRGERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPH-LAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVA---DPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLS-----NCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNG-FRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVG-----------NALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMT-RLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGK-LQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHN--CISHIEDPTF-GAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLR-TSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQ-LNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRD 804
            I  G +   R     L D  + E++  +L  L LSL  L+++GN +T +P G+F +   L  L    N I          G  + L  LDL+      I    +  ++++R L +S  R+ +     + F    + +  L++  +N+  +  +AF +   I Y   + N +  I+ +AF E    LL L      +  I E+ +   + L N L+ LD S N+++ LP   F     I+R+   DN +   P  TF G    T++++N     +  +       +  L  + L   N    +    F +++  L+ +NL  + +  I   AF+++P +  +DL+ N +   +  +F +VG           N +  L ++N +       G     S ++ LDLS N+I  +    F  +   L HLYL +N++    +  F +   P L  LD+  N +   ++D F+  K LQ LL + N I  + +++   + +L I+ L  N++R LPD  F +   ++ LDL++N+   L   +   I T S   +D+S N L    ++ ++T   L  ++ LDLSYN                 +++ L   + S+++         +  LDLSHN   I      TF G  + L  L L++  L S+    LR   RLQ + L+HN L ++P E+    TSL    ++ N L  +P+  +      LK  +L+ N IT I+++  L     L  L++    +S  +A A C           +  L ++H+  + GI++
Sbjct:  307 IEEGTFRNSRIRELYLSDCDLLEINSANLAGLELSLELLDVSGNNITMLPNGLFQEFDFLRTLVFRENRIGTFLPTEVFNGFQYSLYNLDLSGKQNSMISLQDLRQMRNMRSLSIS--RMPQTTLSPNDFLEFGMDIKELRIVNSNLNTIKGHAFMHVRGIKYLDFSENSISTIDDEAFSEVGHSLLTLRMSHGLSSTISEMPNRPFKSLTN-LQHLDFSNNKIRSLPATSFHFLKRIKRIEFQDNEIDNIPKGTFQGDIHSTMEEINFAFNQVKNLQTHTFVDLSALMAINLED-NVIEKIERRTFMNMN-RLKYINLRGNKIKDITDEAFQNLPDLEFLDLAYNNLNEFDFASFDQVGTLSSFKVNVSHNEISRLWVNNTVFTSPFVVGG-NVQSNIKVLDLSYNNISEITKYYFKPVEYSLTHLYLSNNKLKNITQDIFDNM--PHLQWLDMRHNVLTEPDFDCFKNTKNLQVLLFSWNEITDIPAETLRPLKKLRIVDLSHNKLRTLPDNMFTD-SNVESLDLSHNQFTRLPVKSMSVISTASLANLDMSWNILSG--IHSADTIFRLRGLVWLDLSYN-----------------RLVRLDDGVFSDLS--------YLAHLDLSHNKQLILESRGRTFYGLEDTLLYLGLSNISLLSVPELPLR---RLQALYLAHNELASIPPEMSSNLTSLHYLDLSFNDLTVVPL--ITHTLPELKTFNLADNPITAITNTSFLGIADSLEELDIRRLTLSVFEAGALC----------KATKLRKLHITAYNGIKN 1051          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Match: gi|1059881407|ref|XP_017796115.1| (PREDICTED: chaoptin [Habropoda laboriosa])

HSP 1 Score: 515.768 bits (1327), Expect = 3.902e-161
Identity = 335/897 (37.35%), Postives = 519/897 (57.86%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKV----ADPFFIG-QLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQ-FSPNI---------TFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALH--FRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSN-TYPTLSN--------IMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKA-LNPVQSSLKYLDLSANQITTI-SDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNP--LHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLR-HLESLDISGVRAKSFQVRA 916
            L  N+IS+++ ++ R L  SL++L L  NA+  +P   F  L  L+ LDL  NN+ +I    F+ G+ HL  L L  N +  IP+  ++ +K ++ LDLS NRI K+     +P   G Q+ LD L+L  N I+NL A  FQ+F  +N T L GNPL  IE   F +++I+ L  S+C + E+ S    GLE SLE LDLS N +  LP  IF ++D +R L   +N +Q FSP++          FNGF+Y++ +L+L G++   + L+++  MRN+R + +S +    +++TD F +    ++ L +V S+L +I+ +AF HV  I  +D S N I  IE+ AF EVG++L  LRMS+ L      +P GPF+ L+ L+ LD SNN I+SLP  +FH + R++ + LQDNEI   ++GTF    + +L  ++ +FN I+ ++  TF    K+  + L DN I +++ ++F+ M  L  ++L GN+I+ + DE FQNL  L+ LDLAYN L   +FA+FD +GTLS   ++ SHNQ+  L +N +  T PT SN        I  LDLSYNNIS +   +F+PV+  L  L LS N +S +T    G +  ++ LDLSHN +  I+   F     +  L+L++N +  +  + LR  K L++VDLSHN L TLP+ +F   ++E   ++HN+   +P+K       SSL  LDLS N ++ I +   + +++ L +L+L  N++  +D   F  +P L  L+L+ N   L      TF G+ D L  L+++N SL  +P L L  L++L ++ N+L  IP    +NL+ +  LDLS N+LT  P    H++  L + +L+ NPI  V N SF G+   LE LDI  +   +F+  A
Sbjct:  153 LTGNKISKIAADNWRGLENSLQKLRLGRNAIDRLPADAFAGLTYLDMLDLRENNLKEIDPSVFRDGMAHLIYLYLNGNQLTHIPYAQLSSLKRMKELDLSYNRISKMLNLQQEPEIRGLQMSLDVLRLDYNQIENLMAGDFQHFYKVNRTYLDGNPLTMIEEGTFRDSRIRELYFSDCHLMEVGSPDFAGLETSLELLDLSGNNITMLPSPIFQEYDFLRTLIFRENKIQTFSPDLNVTNFTLAEVFNGFQYSLYNLDLSGKENSVINLQDLRQMRNMRFLSISRMPQ-STLSTDDFMEYGMDIKELRIVKSNLNTIKGHAFMHVRGIKYLDFSENSISSIEDEAFSEVGHSLLTLRMSHGLSTSISEIPTGPFKFLTNLQHLDFSNNKIKSLPDTSFHFLKRIKRIELQDNEIDNIRKGTFQGDIHSTLEEVNFAFNKIKTIQTHTFVDLPKVTMINLEDNAIDRIERRAFMNMKLLKYINLRGNKIKDITDEAFQNLPDLEFLDLAYNDLAEFDFASFDQVGTLSSFKVNASHNQIPKLWINSTTFTPPTASNMGGAIQSNIRVLDLSYNNISDIMKFYFKPVEYSLTHLYLSHNQLSNVTQGVFGNMPHLQWLDLSHNELMEIDFDCFRNTKNIQVLFLSYNNIMDIPAEALRPLKMLRIVDLSHNKLRTLPDNMFFDANIESLDLSHNQFMRLPIKTMSLSAASSLSMLDLSWNTLSGIHTTDAIFRLRSLTWLDLSYNRLVRLDDGVFSDMPYLTHLDLSHNKQLLLESRGRTFQGLEDSLLYLDLSNISLLSVPELPLRRLQTLYLANNELASIPAEMASNLTSLHYLDLSANDLTVVPLIT-HTLPELKTFNLADNPITAVSNTSFLGIADSLEELDIRRLSLSTFESGA 1047          

HSP 2 Score: 156.377 bits (394), Expect = 4.286e-35
Identity = 219/906 (24.17%), Postives = 401/906 (44.26%), Query Frame = 0
Query:  117 GVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYT-SLAGNPLEWIEPDAFLETQ-IKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNML-QFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTT-------DQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSL-SALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLS----FNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLE-------GNQIRQL----------PDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNI------SF--------VEV-----------TFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRT-SLEIFKV--AHNRLYEI--------PVKALN---PVQSSLKYLDLSANQITTISDSQLNQIQL-LVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNV---SFNQLTFIPPNTLANLSQVRELDLSGNELTSPP---------------STAW------HSMDHLLSLH------LSGNPIVRVMNDSFAGLRHLESLDIS-------GVRAKSFQ 913
             +P L ++   +  M +IP     +   + +L L NN +     P F+    L  L+++ N + ++   AF   E   +   L  N LE +   ++   Q ++LLDL+   I ++A++  RGLENSL+ L L  N +  LP D F     +  L L +N L +  P++  +G  + I  L L G  + ++P  ++  ++ ++ + LS    Y  ++         +   L  SL+ L L  + + ++    F+H   ++   L  N +  IEE  FR+  + ++ L  S+  H   + +  F  L ++L  LDLS N+I  LP   F +   L+ L  ++N+I TF         +P L + + +    FN  +   Y+    GK +  ++N   ++++ +  F+ ++R+   +L        G  I++L              F ++  ++ LD + N + S+   AF  +G  S L + +SH    ++    +  +  L+N+  LD S N I      SF        +E+           TF   + + L+ +N + N I  I       L ++ +++L  N I  IE   F     L  + L  N++  +  +  +N   L+ +DL++N L       F +  +L  FKV  +HN++ ++        P  A N    +QS+++ LDLS N I+ I       ++  L +L L  N++S +    F  +P+L+ L+L+ N L  I  D F   ++ +Q L ++  ++  +P   L  LK L +   S N+L  +P N   + + +  LDLS N+    P                 +W      H+ D +  L       LS N +VR+ +  F+ + +L  LD+S         R ++FQ
Sbjct:   50 AIPDLGIVTCYNVPMPRIP--RPINSSKVFMLQLENNGL-MFLQPQFLMNTGLYNLRIKHNPLADIPDEAFLGLERSLWELELPYNRLERVPSRSYRHLQKLQLLDLTGNKISKIAADNWRGLENSLQKLRLGRNAIDRLPADAFAGLTYLDMLDLRENNLKEIDPSVFRDGMAHLIY-LYLNGNQLTHIPYAQLSSLKRMKELDLS----YNRISKMLNLQQEPEIRGLQMSLDVLRLDYNQIENLMAGDFQHFYKVNRTYLDGNPLTMIEEGTFRD--SRIRELYFSDC-HLMEVGSPDFAGLETSLELLDLSGNNITMLPSPIFQEYDFLRTLIFRENKIQTF---------SPDLNVTNFTLAEVFNGFQYSLYNLDLSGK-ENSVINLQDLRQMRNMRFLSISRMPQSTLSTDDFMEYGMDIKELRIVKSNLNTIKGHAFMHVRGIKYLDFSENSISSIEDEAFSEVGH-SLLTLRMSHGLSTSISEIPTGPFKFLTNLQHLDFSNNKIKSLPDTSFHFLKRIKRIELQDNEIDNIRKGTFQGDIHSTLEEVNFAFNKIKTIQTHTFVDLPKVTMINLEDNAIDRIERRAFMNMKLLKYINLRGNKIKDITDEAFQNLPDLEFLDLAYNDLAEFDFASFDQVGTLSSFKVNASHNQIPKLWINSTTFTPPTASNMGGAIQSNIRVLDLSYNNISDIMKFYFKPVEYSLTHLYLSHNQLSNVTQGVFGNMPHLQWLDLSHNELMEIDFDCFRNTKN-IQVLFLSYNNIMDIPAEALRPLKMLRIVDLSHNKLRTLPDNMFFD-ANIESLDLSHNQFMRLPIKTMSLSAASSLSMLDLSWNTLSGIHTTDAIFRLRSLTWLDLSYNRLVRLDDGVFSDMPYLTHLDLSHNKQLLLESRGRTFQ 931          

HSP 3 Score: 149.828 bits (377), Expect = 5.295e-33
Identity = 201/771 (26.07%), Postives = 334/771 (43.32%), Query Frame = 0
Query:   37 ISLGLWVGGRGERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHS-----------MAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVA-DPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLS-----NCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNG-FRYTIKDLNLLG------QDMYYVPLEEIGI------------------MRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDL--SNNRIE--YIEENAF-----REVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMT-RLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGK-LQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRN---IISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQL 751
            I  G +   R       D  + E+   D   L  SL  L+L+GN +T +P  IF +   L  L + R N IQ  S             F      L  LDL+      I    +  ++++R L +S      ++ D F    + +  L++ ++N+  +  +AF +   I Y   + N +  IE +AF E    LL L      +  I E+ +   + L N L+ LD S N+++ LP+  F     I+R+ L DN +      TF G    T++++N         Q   +V L ++ +                  M+ L+ + L   N    +T + F +L P LE L+L  +DL   +  +F  V ++SS  +  S+N+I   +I    F       +G A+Q                     S +R LDLS N+I  +    F  +   L HLYL  N++S   +G F +   P L  LDLS N +  +++D FR  K +Q L L+ N I  + +++   +  L I+ L  N++R LPD  F + + ++ LDL++N+   L           S   +DLS N L    ++ ++    L ++  LDLSYN +  ++   F  +   L  L+LS N   ++        G    +  LDLS+  IS +  P      +L +LYL +N L S+  ++  N   L  +DLS N L  +P        L+ F +A N +  +   +   +  SL+ LD+    ++T     L
Sbjct:  312 IEEGTFRDSRIRELYFSDCHLMEVGSPDFAGLETSLELLDLSGNNITMLPSPIFQEYDFLRTL-IFRENKIQTFSPDLNVTNFTLAEVFNGFQYSLYNLDLSGKENSVINLQDLRQMRNMRFLSISRMPQSTLSTDDFMEYGMDIKELRIVKSNLNTIKGHAFMHVRGIKYLDFSENSISSIEDEAFSEVGHSLLTLRMSHGLSTSISEIPTGPFKFLTN-LQHLDFSNNKIKSLPDTSFHFLKRIKRIELQDNEIDNIRKGTFQGDIHSTLEEVNFAFNKIKTIQTHTFVDLPKVTMINLEDNAIDRIERRAFMNMKLLKYINLRG-NKIKDITDEAFQNL-PDLEFLDLAYNDLAEFDFASFDQVGTLSSFKVNASHNQIPKLWINSTTFTPPTASNMGGAIQ---------------------SNIRVLDLSYNNISDIMKFYFKPVEYSLTHLYLSHNQLSNVTQGVFGNM--PHLQWLDLSHNELMEIDFDCFRNTKNIQVLFLSYNNIMDIPAEALRPLKMLRIVDLSHNKLRTLPDNMFFDAN-IESLDLSHNQFMRLPIKTMSLSAASSLSMLDLSWNTLSG--IHTTDAIFRLRSLTWLDLSYNRLVRLDDGVFSDMPY-LTHLDLSHNKQLLLESRGRTFQGLEDSLLYLDLSN--ISLLSVPEL-PLRRLQTLYLANNELASIPAEMASNLTSLHYLDLSANDLTVVPLITHTLPELKTFNLADNPITAVSNTSFLGIADSLEELDIRRLSLSTFESGAL 1048          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Match: gi|332022695|gb|EGI62972.1| (Chaoptin [Acromyrmex echinatior])

HSP 1 Score: 513.842 bits (1322), Expect = 9.956e-161
Identity = 331/890 (37.19%), Postives = 520/890 (58.43%), Query Frame = 0
Query:   48 ERCALGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKV----ADPFFIG-QLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQ-FSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALH--FRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTF-RFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPT-------LSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSS-LKYLDLSANQITTI-SDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNP--LHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLR-HLESLDISGVRAKSFQVRA 916
            E   L  N+IS +  E+ R L  SL+ L L  NA+  +P   F  L  LE LDL  N++ +I S  F+ G+ HL  L L DN +  +P+I +  +K ++VLDLS N+I K+     +P   G Q+ LD LQL  N I+ L  + FQ+F  +N+T L GNPL  IE   F +++I+ L LS+C + E+ S  L GLE+SLE LD+S N +  LP  +F +FD +R L   +N +  FSP   F+GF+Y + +L+L G+    + L+++  MRNLR + +S +     ++ + F +    ++ L +++S+L +I+++AF HV  I  +D S N I  I+++AF EVG +L  LRMS+ L      +PN PF+SL+ L+ LDLSNN IRSL   +FH + R++ + LQDNEI +  +GTF    + +L  ++ +FN +  ++  TF     L  + L DN I++++ ++F+ M RL  ++L GN+IR + DE FQNL  L+ LDLAYN L   +FA+FD +GTLS   +++SHN++  L +N ++   +        SNI  LDLSYNNIS +   +F+PV+  L  L LS N +  IT +  G +  ++ LDL HN +  I        N L  L  + N +T +  + LR+ K+L++VDLSHN L TLP+ +F+ + +E   ++HN+   +PVK+++   ++ L  LD+S N ++ I +   + +++ LV+L+L  N++  +D   F  +P L  L+L+ N   +      TF G+ D L  L ++N SL  +P L L  L++LN++ N L  I P   +NL+ +  LDLS N+LT  P    H++  L + +L+ NPI  + N SF G+   LE LDI  +    F+  A
Sbjct:  137 EVLELTGNKISRIVPENWRGLENSLKTLRLGKNAIEKLPADAFVSLTYLEILDLRENSLKEIDSSVFRDGMAHLTYLYLNDNQLTYVPYIQLFSLKRMKVLDLSYNKISKMLHAQQEPEVRGIQMSLDVLQLDYNQIETLAFSDFQHFYKVNHTYLRGNPLMTIEEGTFKDSRIRELYLSDCDLLEINSANLAGLESSLELLDVSGNNITVLPNRLFQEFDLLRTLIFRENRINTFSPTEVFSGFQY-LYNLDLSGKQNGMISLQDLRQMRNLRFLSISRMPQ-AILSANDFLEFGMDIKELRIINSNLNTIKNHAFMHVRGIKYLDFSENSISTIDDDAFSEVGYSLLTLRMSHGLSSSISEIPNQPFKSLTNLQHLDLSNNKIRSLSATSFHFLKRIKRIELQDNEIDSILKGTFQGDIHSTLEEINFAFNQVRDLQTHTFIDLSALMTINLEDNTIERIERRAFINMNRLKYINLRGNKIRDITDEAFQNLPDLEFLDLAYNNLNEFDFASFDQVGTLSSFKVNVSHNEISRLWINSTSFISSSVVNGNVQSNIKVLDLSYNNISDIAKYYFKPVEYSLTHLYLSNNQLRNITQNVFGNMPHLQWLDLRHNELIEIYFDCLKNTNNLQVLLFSWNEITDIPAETLRSLKKLRIVDLSHNKLRTLPDNMFIDSDIESLDLSHNQFTRLPVKSMSVTSTANLVNLDMSWNILSGIHTTDTIFRLRGLVWLDLSYNRLVRLDDGVFSDLPYLTHLDLSHNKQLILESRGRTFYGLEDTLLYLGLSNISLLSIPELPLRRLQTLNLAHNGLASISPEMSSNLTSLHYLDLSYNDLTVIPLIT-HTLPELKTFNLANNPITAITNTSFLGVADSLEELDIRKLSLSIFEAGA 1023          

HSP 2 Score: 157.147 bits (396), Expect = 2.965e-35
Identity = 213/850 (25.06%), Postives = 385/850 (45.29%), Query Frame = 0
Query:  117 GVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEY-INYTSLAGNPLEWIEPDAFLETQ-IKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSP-------SLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHS------------QANPSLAILD------LSFNHIERVEY------DTFRFG-KLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNL-HRLQRLDLAYNRLESLNF---AAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNI-SFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRT-SLEIFK--VAHNRLYEIPVKALN---------PVQSSLKYLDLSANQITTISDSQLNQIQL-LVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNV---SFNQLTFIPPNTLANLSQVRELDLSGNELTSPP--STAWHSMDHLLSLHLSGNPIVRV-MNDSFAGLRHLESLDISGVR 908
             +P L  +      M +IP     +   + +L L NN + +   P ++    L  LQ++ N + ++   AF   E  ++   L+ N L  I   +F   Q +++L+L+   I  +     RGLENSL+ L L  N +++LP D F+    +  L L +N L+   +  F      +  L L    + YVP  ++  ++ ++ + LS    Y  ++    A   P       SL+ L L  + + ++  + F+H   ++   L  N +  IEE  F++      YL   + L   +         S+L  LD+S N+I  LP   F +   L+ L  ++N I+TF      S            + N  +++ D      L F  I R+        D   FG  +++L + ++ +  + + +F+ +  +  L    N I  + D+ F  + + L  L +++    S++      F S+  L HL  DLS+N++++L    + ++  L  I  ++L  N I S ++ TF   + + L+ +N + N + ++       L  +  ++L  N I  IE   F   N+L  + L  N++  +  +  +N   L+ +DL++N L       F +  +L  FK  V+HN +  + + + +          VQS++K LDLS N I+ I+      ++  L +L L  N++  I  + F  +P+L+ L+L  N L  I+ D      ++LQ L  +   +T +P   L SLK L +   S N+L  +P N   + S +  LDLS N+ T  P  S +  S  +L++L +S N +  +   D+   LR L  LD+S  R
Sbjct:   38 AMPDLGRVACYSVPMPRIP--QPINSSKMYMLQLENNGL-RFLQPQYLMNTGLYQLQIKHNPLADIPEEAFLGLERSLSMLDLSYNQLASIPSKSFRHLQKLEVLELTGNKISRIVPENWRGLENSLKTLRLGKNAIEKLPADAFVSLTYLEILDLRENSLKEIDSSVFRDGMAHLTYLYLNDNQLTYVPYIQLFSLKRMKVLDLS----YNKISKMLHAQQEPEVRGIQMSLDVLQLDYNQIETLAFSDFQHFYKVNHTYLRGNPLMTIEEGTFKDSRIRELYLSDCDLLEINSANLAGLE--SSLELLDVSGNNITVLPNRLFQEFDLLRTLIFRENRINTFSPTEVFSGFQYLYNLDLSGKQNGMISLQDLRQMRNLRFLSISRMPQAILSANDFLEFGMDIKELRIINSNLNTIKNHAFMHVRGIKYLDFSENSISTIDDDAFSEVGYSLLTLRMSHGLSSSISEIPNQPFKSLTNLQHL--DLSNNKIRSL---SATSFHFLKRIKRIELQDNEIDSILKGTFQGDIHSTLEEINFAFNQVRDLQTHTFIDLSALMTINLEDNTIERIERRAFINMNRLKYINLRGNKIRDITDEAFQNLPDLEFLDLAYNNLNEFDFASFDQVGTLSSFKVNVSHNEISRLWINSTSFISSSVVNGNVQSNIKVLDLSYNNISDIAKYYFKPVEYSLTHLYLSNNQLRNITQNVFGNMPHLQWLDLRHNELIEIYFDCLKNT-NNLQVLLFSWNEITDIPAETLRSLKKLRIVDLSHNKLRTLPDNMFID-SDIESLDLSHNQFTRLPVKSMSVTSTANLVNLDMSWNILSGIHTTDTIFRLRGLVWLDLSYNR 871          

HSP 3 Score: 105.916 bits (263), Expect = 2.274e-19
Identity = 156/598 (26.09%), Postives = 269/598 (44.98%), Query Frame = 0
Query:  353 IEHNAFKHVP---------SISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSL-SALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEI-----STFKRGTFHSQANPSLAILDLSFNHIERVEY-DTFRFGKLQQLLLNDNRIKK-LDSQSFVEM----TRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDV-GQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEI--FKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQL-LVYLNLE---GNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGI----RDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRASD 918
            I+HN    +P         S+S +DLS N++  I   +FR +   L+ L ++     R +P   +R L ++L+TL L  N I  LP D F  +T L+ L L++N +     S F+ G  H      L  L L+ N +  V Y   F   +++ L L+ N+I K L +Q   E+      L +L L+ NQI  L    FQ+ +++    L  N L ++    F      S +  +L  +    L +N +N     S++  LD+S NNI+ +    F+   + L+ L    N I+  +P +V    + +  LDLS            G  N + SL            + LR  + L+ + +S      L    FL   ++I   ++ ++ L  I   A   V+  +KYLD S N I+TI D   +++   L+ L +     + IS I    F  + NL+ L+L++N +  +   +F  +    R  LQ   + +           S+L+ +N +FNQ+  +  +T  +LS +  ++L  N +      A+ +M+ L  ++L GN I  + +++F  L  LE LD++      F   + D
Sbjct:   92 IKHNPLADIPEEAFLGLERSLSMLDLSYNQLASIPSKSFRHL-QKLEVLELTGNKISRIVPEN-WRGLENSLKTLRLGKNAIEKLPADAFVSLTYLEILDLRENSLKEIDSSVFRDGMAH------LTYLYLNDNQLTYVPYIQLFSLKRMKVLDLSYNKISKMLHAQQEPEVRGIQMSLDVLQLDYNQIETLAFSDFQHFYKVNHTYLRGNPLMTIEEGTFKD----SRIR-ELYLSDCDLLEINSANLAGLESSLELLDVSGNNITVLPNRLFQEF-DLLRTLIFRENRINTFSPTEVFSGFQYLYNLDLS------------GKQNGMISL------------QDLRQMRNLRFLSISRMPQAILSANDFLEFGMDIKELRIINSNLNTIKNHAFMHVRG-IKYLDFSENSISTIDDDAFSEVGYSLLTLRMSHGLSSSISEIPNQPFKSLTNLQHLDLSNNKIRSLSATSFHFLKRIKRIELQDNEIDSILKGTFQGDIHSTLEEINFAFNQVRDLQTHTFIDLSALMTINLEDNTIERIERRAFINMNRLKYINLRGNKIRDITDEAFQNLPDLEFLDLAYNNLNEFDFASFD 650          
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Match: gi|1061479428|gb|ODM96941.1| (Chaoptin [Orchesella cincta])

HSP 1 Score: 514.998 bits (1325), Expect = 1.075e-160
Identity = 332/892 (37.22%), Postives = 526/892 (58.97%), Query Frame = 0
Query:   52 LGDNQISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIGQLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYG--SVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNT--------------------YPTL-----SNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSR-NIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQ-LSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQV 914
            LG N+I+++  +DL  L  S+  L L+GN +T + E  F  L RLE LDLS N+I  IH+ AF +    L +L L +NL  KIPF +++ ++ L+ LDLS+N +  + D F+  ++ +  L+L  N I ++   AFQNF   +  SLA NP+E +  DAF +  I LLDLS+C I+E+   A RGLENSLE+LD+S N +  +P ++F +FD +R L L+ N LQ + +   N   +T+   +++G  M    +E+I +MRNLR++ +S   N+G  S+    F     ++E + +    L++I+  AF  +  + S+DLS NRIE IE  AF  V ++L  L+M+N++    LP+  F+ LSALR LDLS N + ++P DTF +  RLQ+L L+ N++S+  +  F     P +  + LSFN+I+ V     R  +L+ + LN+N I+ +  ++F ++T L IL+L GN+I  L DE FQNL RLQ LDL++NRL S+N+ AF+ +GTLS   I LSHN +Q L ++  ++                     P L     S+I S+DLSYNNISF+   FF P+ + L  L+LS  N +  IT +++  +R I+ + L+HN +  +E  +F  +  +  + ++HN L  +   L      L+V+  + N +  L + +F  T LEI  V++N+L   P  AL  V ++L  LDLS N+I++++ SQ+  +Q L +L+L  N+I  +  ++F  + +L  L+L+ N L ++    F GI++ L  L++AN SL  LP     + L + N S+N+LT++P N   N+S +R LD+SGN++ +PP+T WH++  L  + +  NPI  + NDSF  L  L  LDI+ +  +S QV
Sbjct:  176 LGYNKITDVYPDDLAGLPTSITRLGLSGNMITYIQENAFASLPRLESLDLSDNSIQFIHNQAFASLSKSLKLLYLDNNLFLKIPFQALSPLE-LKTLDLSHNLLTSMFDIFYEKKITVGVLKLNYNRITSVPPYAFQNFRKASVVSLAFNPIESLVDDAFKDASIDLLDLSHCFIREVQPKAFRGLENSLESLDISFNNITHIPFELFENFDRLRHLNLDQNRLQITLDELENFRLFTLYRFSIIGDMMSPFSMEDIELMRNLRSLNIS---NFGMASLNPQVFEGFGLAVEDIKISQGKLSTIKAGAFSSLTGLKSIDLSENRIERIENQAFEGVEHSLLRLKMANSIRMGTLPSKAFQKLSALRYLDLSANGLNNVPEDTFAESKRLQYLNLRFNKLSSVPKNMFDCTKVPRIQTILLSFNNIKEVRSGFVRLKELEIVELNENVIETISPRAFEDLTNLRILNLAGNKISILEDEAFQNLPRLQILDLSHNRLSSINWEAFEQLGTLSSFRISLSHNNIQRLLISTEDSQIQDTDISGEDGSKEDDPIPVPVLSSISSSSIESIDLSYNNISFISPHFFFPISSTLIELDLSHNNRLQNITLENLSGMRFIQSIQLNHNSLDTLEYNSFRESKYIQIINMDHNMLRDIPPDLFEGCVNLRVLSATDNKIRALTDSIFKDTQLEILNVSNNQLVRFPENALARVATTLVQLDLSGNEISSLTASQVECLQKLRWLDLSNNRIVVVAENSFDNLYSLLHLDLSGNSLGKLSARFFEGIKETLLHLHIANMSLDALPEFDTFAKLLTFNSSYNRLTYLPTNFGVNVSSLRTLDISGNQIPAPPNTIWHTIPRLSQVFMQDNPIRSLTNDSFLQLERLHELDITDLPLESAQV 1063          

HSP 2 Score: 139.428 bits (350), Expect = 8.014e-30
Identity = 228/913 (24.97%), Postives = 403/913 (44.14%), Query Frame = 0
Query:   57 ISELSREDLRSLALSLRELNLAGNALTSVPEGIFWDLQRLERLDLSRNNIIQIHSMAFQTGVPHLAVLDLADNLMGKIPFISIAHVKSLRVLDLSNNRIEKVADPFFIG-QLHLDTLQLRENNIQNLTANAFQNFEYINYTSLAGNPLEWIEPDAF--LETQIKLLDLSNCL-IQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEI--GIMR--NLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSALRTLDLSNNHIRSLPLDTFHKMTRLQHLYLQDNEIS----------------------------------TFKRGTFHSQANPSLAILDLSFNH----IERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAI-DLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKR----LQVVDLSHNFLETLPE-ELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLS-----QVRELDLSGNELTSPPSTAWHSMD-HLLSLHLSGNPIVRVMNDSFAGLRHLE-----SLDISG 906
            I E+  +  R L  SL  L+++ N +T +P  +F +  RL  L+L +N +           +  L    +  ++M       I  +++LR L++SN  +  +    F G  L ++ +++ +  +  + A AF +   +    L+ N +E IE  AF  +E  +  L ++N + +  L S A + L ++L  LDLSAN L  +PED F +   ++ L L  N L   P   F+  +       LL     +  ++E+  G +R   L  V L+  N   +++   F DL+ +L  LNL  + ++ +E  AF+++P +  +DLS+NR+  I   AF ++G                       +LS+ R + LS+N+I+ L + T       +   +QD +IS                                  +F    F    + +L  LDLS N+    I        RF  +Q + LN N +  L+  SF E   + I++++ N +R +P + F+    L+ L    N++ +L     DSI   + L I ++S+NQL                   +    N ++ V  T  +        L+LS N IS +T   V  L+++R LDLS+N I  + + +F     L  L L+ N L  L  +     K     L + ++S   L+ LPE + F +  L  F  ++NRL  +P      V SSL+ LD+S NQI    ++  + I  L  + ++ N I ++  D+F  +  L +L++   PL    +  F  +   ++ ++    S ++ P L + +++SL   + +L  +P  ++   S     ++  + +SG EL S    A+  ++   L L +    I  + ND    L HL      SL I+G
Sbjct:  349 IREVQPKAFRGLENSLESLDISFNNITHIPFELFENFDRLRHLNLDQNRLQITLDELENFRLFTLYRFSIIGDMMSPFSMEDIELMRNLRSLNISNFGMASLNPQVFEGFGLAVEDIKISQGKLSTIKAGAFSSLTGLKSIDLSENRIERIENQAFEGVEHSLLRLKMANSIRMGTLPSKAFQKL-SALRYLDLSANGLNNVPEDTFAESKRLQYLNLRFNKLSSVPKNMFDCTKVPRIQTILLS----FNNIKEVRSGFVRLKELEIVELNE-NVIETISPRAFEDLT-NLRILNLAGNKISILEDEAFQNLPRLQILDLSHNRLSSINWEAFEQLG-----------------------TLSSFR-ISLSHNNIQRLLIST-------EDSQIQDTDISGEDGSKEDDPIPVPVLSSISSSSIESIDLSYNNISFISPHFFFPISSTLIELDLSHNNRLQNITLENLSGMRF--IQSIQLNHNSLDTLEYNSFRESKYIQIINMDHNMLRDIPPDLFEGCVNLRVLSATDNKIRALT----DSIFKDTQLEILNVSNNQL-------------------VRFPENALARVATTLVQ--------LDLSGNEISSLTASQVECLQKLRWLDLSNNRIVVVAENSFDNLYSLLHLDLSGNSLGKLSARFFEGIKETLLHLHIANMS---LDALPEFDTFAK--LLTFNSSYNRLTYLPTNFGVNV-SSLRTLDISGNQIPAPPNTIWHTIPRLSQVFMQDNPIRSLTNDSFLQLERLHELDITDLPLESAQVGVFTPLV-CMKKISFGPFSSSISPGLIIQAVESLQSLYLKLD-VPMMSVELTSYPLPEKISTITVSGEELLSISGGAFDEVEGRQLKLIIDNTGITDLPNDV---LHHLGDATQLSLKITG 1179          

HSP 3 Score: 139.043 bits (349), Expect = 1.131e-29
Identity = 184/731 (25.17%), Postives = 330/731 (45.14%), Query Frame = 0
Query:  197 SLAGNPLEWIEPDAFLETQIKLLDLSNCLIQELASNALRGLENSLEALDLSANRLQELPEDIFMDFDTIRRLRLNDNMLQFSPNITFNGFRYTIKDLNLLGQDMYYVPLEEIGIMRNLRTVGLSSVNNYGSVTTDQFADLSPSLEALNLVDSDLTSIEHNAFKHVPSISSMDLSNNRIEYIEENAFREVGNALQYLRMSNALHFRALPNGPFRSLSA--LRTLDLSNNHIRSLPLDTFH-KMTRLQHLYLQDNEISTFKRGTFHSQANPSLAILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEM-TRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLE-------------------------------------------------SLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQ-LRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQK--RLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKISTIDADAFCCVPNLE--QLNLASNPLHRIHLDTFGGIRDHLQGLNVAN---------TSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLSQ-VRELDLSGN 859
            S+ GN ++ ++    + + +  L++    +  +A +A  GL   L  LDL  N+L  +P        ++RRL                        L +L          E+G             N    V  D  A L  S+  L L  + +T I+ NAF  +P + S+DLS+N I++I   AF  +  +L+ L + N L  +     PF++LS   L+TLDLS+N + S+  D F+ K   +  L L  N I++     F +    S  ++ L+FN IE +  D F+   +  L L+   I+++  ++F  +   L  L +  N I  +P E F+N  RL+ L+L  NRL+                                                 SLN   F+  G L+   I +S  +L  +   ++  + +L+ + S+DLS N I  +E   FE V++ L  L ++ +I     P    Q L  +R LDLS N ++++ + TF  + +L  L L  N+L+S+   +    K  R+Q + LS N ++ +         LEI ++  N +  I  +A   + ++L+ L+L+ N+I+ + D     +  L  L+L  N++S+I+ +AF  +  L   +++L+ N + R+ + T       +Q  +++            + +L ++  SS++S+++S+N ++FI P+    +S  + ELDLS N
Sbjct:  100 SMVGNNMQVLDAKRLVGSGLWRLEIKENELNYVADDAFVGLSKFLAELDLQCNQLTSIPT------SSLRRLA-------------------KPAKLKIL----------ELG------------YNKITDVYPDDLAGLPTSITRLGLSGNMITYIQENAFASLPRLESLDLSDNSIQFIHNQAFASLSKSLKLLYLDNNLFLKI----PFQALSPLELKTLDLSHNLLTSM-FDIFYEKKITVGVLKLNYNRITSVPPYAFQNFRKAS--VVSLAFNPIESLVDDAFKDASIDLLDLSHCFIREVQPKAFRGLENSLESLDISFNNITHIPFELFENFDRLRHLNLDQNRLQITLDELENFRLFTLYRFSIIGDMMSPFSMEDIELMRNLRSLNISNFGMASLNPQVFEGFG-LAVEDIKISQGKLSTI---KAGAFSSLTGLKSIDLSENRIERIENQAFEGVEHSLLRLKMANSIRMGTLPSKAFQKLSALRYLDLSANGLNNVPEDTFAESKRLQYLNLRFNKLSSVPKNMFDCTKVPRIQTILLSFNNIKEVRSGFVRLKELEIVELNENVIETISPRAFEDL-TNLRILNLAGNKISILEDEAFQNLPRLQILDLSHNRLSSINWEAFEQLGTLSSFRISLSHNNIQRLLISTEDS---QIQDTDISGEDGSKEDDPIPVPVLSSISSSSIESIDLSYNNISFISPHFFFPISSTLIELDLSHN 768          

HSP 4 Score: 105.916 bits (263), Expect = 1.887e-19
Identity = 119/491 (24.24%), Postives = 236/491 (48.07%), Query Frame = 0
Query:  466 ILDLSFNHIERVEYDTFRFGKLQQLLLNDNRIKKLDSQSFVEMTRLLI-LSLEGNQIRQLPDETFQNLHR---LQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFGAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKIS-TIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLS-----SLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNE--------------------------LTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRASDCS 920
            +L +  N+++ ++        L +L + +N +  +   +FV +++ L  L L+ NQ+  +P  + + L +   L+ L+L YN++  +       + T S   + LS N +  ++    N + +L  + SLDLS N+I F+    F  +   LK+L L  N+  +I    +  L  ++ LDLSHN ++ + D  +     +  L LN+NR+TS+     +N ++  VV L+ N +E+L ++ F   S+++  ++H  + E+  KA   +++SL+ LD+S N IT I          L +LNL+ N++  T+D      +  L + ++  + +    ++    +R+ L+ LN++N  +  L N Q+      +++ + +S  +L+ I     ++L+ ++ +DLS N                           + + PS A+  +  L  L LS N +  V  D+FA  + L+ L++   +  S      DC+
Sbjct:   98 LLSMVGNNMQVLDAKRLVGSGLWRLEIKENELNYVADDAFVGLSKFLAELDLQCNQLTSIPTSSLRRLAKPAKLKILELGYNKITDVYPDDLAGLPT-SITRLGLSGNMITYIQ---ENAFASLPRLESLDLSDNSIQFIHNQAFASLSKSLKLLYLDNNLFLKIPFQALSPLE-LKTLDLSHNLLTSMFDIFYEKKITVGVLKLNYNRITSVPPYAFQNFRKASVVSLAFNPIESLVDDAFKDASIDLLDLSHCFIREVQPKAFRGLENSLESLDISFNNITHIPFELFENFDRLRHLNLDQNRLQITLDELENFRLFTLYRFSIIGDMMSPFSMEDIELMRN-LRSLNISNFGMASL-NPQVFEGFGLAVEDIKISQGKLSTIKAGAFSSLTGLKSIDLSENRIERIENQAFEGVEHSLLRLKMANSIRMGTLPSKAFQKLSALRYLDLSANGLNNVPEDTFAESKRLQYLNLRFNKLSSVPKNMFDCT 581          

HSP 5 Score: 103.219 bits (256), Expect = 1.390e-18
Identity = 142/566 (25.09%), Postives = 253/566 (44.70%), Query Frame = 0
Query:  412 SALRTLDLSNNHIRSLPLDTFHKMTR-LQHLYLQDNEISTFKRGTFHSQANPS-LAILDLSFNHIERVEYDTFRF--GKLQQLLLNDNRIKKLDSQSFVEMTRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHLAIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTF-GAANKLSSLYLNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEEL--FLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVY-LNLEGNKISTIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANT-SLTLLPNL---QLSSLKSLNVSFNQLTFIPPNTLANLSQVRELDLSGNELTSPPST-------------------------------------------------AWHSMDHLLSLHLSGNPIVRVMNDSFAGLRHLESLDISGVRAKSFQVRA 916
            S L  L++  N +  +  D F  +++ L  L LQ N++++    +    A P+ L IL+L +N I  V  D        + +L L+ N I  +   +F  + RL  L L  N I+ + ++ F +L +  +L    N L       F ++  L    +DLSHN L ++       Y     +  L L+YN I+ V    F+  +    +++L+ N I  +  DD  +   I +LDLSH  I  ++   F G  N L SL ++ N +T +  +L  N  RL+ ++L  N L+   +EL  F   +L  F +  + +    ++ +  +  +L+ L++S   + +++        L V  + +   K+STI A AF  +  L+ ++L+ N + RI    F G+   L  L +AN+  +  LP+    +LS+L+ L++S N L  +P +T A   +++ L+L  N+L+S P                                                   A+  + +L  L+L+GN I  + +++F  L  L+ LD+S  R  S    A
Sbjct:  117 SGLWRLEIKENELNYVADDAFVGLSKFLAELDLQCNQLTSIPTSSLRRLAKPAKLKILELGYNKITDVYPDDLAGLPTSITRLGLSGNMITYIQENAFASLPRLESLDLSDNSIQFIHNQAFASLSKSLKLLYLDNNL--FLKIPFQALSPLELKTLDLSHNLLTSMF---DIFYEKKITVGVLKLNYNRITSVPPYAFQNFRKA-SVVSLAFNPIESLV-DDAFKDASIDLLDLSHCFIREVQPKAFRGLENSLESLDISFNNITHIPFELFENFDRLRHLNLDQNRLQITLDELENFRLFTLYRFSIIGDMMSPFSMEDIE-LMRNLRSLNISNFGMASLNPQVFEGFGLAVEDIKISQGKLSTIKAGAFSSLTGLKSIDLSENRIERIENQAFEGVEHSLLRLKMANSIRMGTLPSKAFQKLSALRYLDLSANGLNNVPEDTFAESKRLQYLNLRFNKLSSVPKNMFDCTKVPRIQTILLSFNNIKEVRSGFVRLKELEIVELNENVIETISPRAFEDLTNLRILNLAGNKISILEDEAFQNLPRLQILDLSHNRLSSINWEA 674          

HSP 6 Score: 95.5153 bits (236), Expect = 3.803e-16
Identity = 116/464 (25.00%), Postives = 203/464 (43.75%), Query Frame = 0
Query:  509 TRLLILSLEGNQIRQLPDETFQNLHRLQRLDLAYNRLESLNFAAFDSIGTLSHL--AIDLSHNQLQALRVNRSNTYPTLSNIMSLDLSYNNISFVEVTFFEPVQNDLKILNLSRNIISEITPDDVGQLRRIRVLDLSHNCISHIEDPTFG----------------------------------AANKLSSLY--------------LNHNRLTSLHGKLLRNQKRLQVVDLSHNFLETLPEELFLRTSLEIFKVAHNRLYEIPVKALNPVQSSLKYLDLSANQITTISDSQLNQIQLLVYLNLEGNKIS-TIDADAFCCVPNLEQLNLASNPLHRIHLDTFGGIRDHLQGLNVANTSLTLLPNLQLSSLKSLNVSFNQLTFIPPNTLANLS-QVRELDLSGNELTSPPSTAWHSMDHLLSLHLSGNPIVRVMNDSFAGLRH-------LESLDISGVRAKSFQ 913
            +R  +LS+ GN ++ L  +       L RL++  N L   N+ A D+   LS     +DL  NQL ++  +        + +  L+L YN I+ V       +   +  L LS N+I+ I  +    L R+  LDLS N I  I +  F                                   + N L+S++              LN+NR+TS+     +N ++  VV L+ N +E+L ++ F   S+++  ++H  + E+  KA   +++SL+ LD+S N IT I          L +LNL+ N++  T+D         LE   L +  L+R     F  I D +   ++ +  L       + +L+SLN+S   +  + P         V ++ +S  +L++  + A+ S+  L S+ LS N I R+ N +F G+ H         S+ +  + +K+FQ
Sbjct:   94 SRAFLLSMVGNNMQVLDAKRLVG-SGLWRLEIKENEL---NYVADDAFVGLSKFLAELDLQCNQLTSIPTSSLRRLAKPAKLKILELGYNKITDVYPDDLAGLPTSITRLGLSGNMITYIQENAFASLPRLESLDLSDNSIQFIHNQAFASLSKSLKLLYLDNNLFLKIPFQALSPLELKTLDLSHNLLTSMFDIFYEKKITVGVLKLNYNRITSVPPYAFQNFRKASVVSLAFNPIESLVDDAFKDASIDLLDLSHCFIREVQPKAFRGLENSLESLDISFNNITHIPFELFENFDRLRHLNLDQNRLQITLD--------ELENFRLFT--LYR-----FSIIGDMMSPFSMEDIEL-------MRNLRSLNISNFGMASLNPQVFEGFGLAVEDIKISQGKLSTIKAGAFSSLTGLKSIDLSENRIERIENQAFEGVEHSLLRLKMANSIRMGTLPSKAFQ 531          
The following BLAST results are available for this feature:
BLAST of hypothetical protein DAPPUDRAFT_300683 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides)
Total hits: 25
Match NameE-valueIdentityDescription
EMLSAG000000091604.257e-9435.07supercontig:LSalAtl2s:LSalAtl2s577:434769:489672:1... [more]
EMLSAG000000095852.732e-9029.91supercontig:LSalAtl2s:LSalAtl2s616:5915:14813:-1 g... [more]
EMLSAG000000098291.395e-5527.84supercontig:LSalAtl2s:LSalAtl2s63:1003125:1030351:... [more]
EMLSAG000000099832.057e-4125.89supercontig:LSalAtl2s:LSalAtl2s652:91522:194127:1 ... [more]
EMLSAG000000076921.085e-4026.19supercontig:LSalAtl2s:LSalAtl2s447:512720:516392:1... [more]
EMLSAG000000109691.155e-3825.89supercontig:LSalAtl2s:LSalAtl2s736:33395:37479:1 g... [more]
EMLSAG000000080166.561e-3727.33supercontig:LSalAtl2s:LSalAtl2s477:388792:422860:1... [more]
EMLSAG000000087642.263e-3429.36supercontig:LSalAtl2s:LSalAtl2s543:55755:60198:-1 ... [more]
EMLSAG000000121073.631e-3326.51supercontig:LSalAtl2s:LSalAtl2s869:139642:146365:-... [more]
EMLSAG000000037513.317e-2425.29supercontig:LSalAtl2s:LSalAtl2s201:72137:78450:1 g... [more]

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BLAST of hypothetical protein DAPPUDRAFT_300683 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
gi|14424439|sp|P12024.2|CHAO_DROME3.217e-15036.00RecName: Full=Chaoptin; AltName: Full=Photorecepto... [more]
gi|14423663|sp|P82963.1|CHAO_TRICA7.015e-8737.04RecName: Full=Chaoptin; AltName: Full=Photorecepto... [more]
gi|75027275|sp|Q9VPF0.2|ATK_DROME1.021e-4927.46RecName: Full=Protein artichoke; Flags: Precursor[more]
gi|2498124|sp|P70389.1|ALS_MOUSE5.528e-3731.55RecName: Full=Insulin-like growth factor-binding p... [more]
gi|543801|sp|P35859.1|ALS_RAT1.492e-3529.51RecName: Full=Insulin-like growth factor-binding p... [more]
gi|2498123|sp|O02833.1|ALS_PAPHA1.190e-3328.49RecName: Full=Insulin-like growth factor-binding p... [more]
gi|122126327|sp|Q7KIN0.1|TOLL7_DROME8.708e-3226.35RecName: Full=Toll-like receptor 7; Flags: Precurs... [more]
gi|543800|sp|P35858.1|ALS_HUMAN1.695e-3128.09RecName: Full=Insulin-like growth factor-binding p... [more]
gi|45477220|sp|O88280.1|SLIT3_RAT1.483e-2723.96RecName: Full=Slit homolog 3 protein; Short=Slit-3... [more]
gi|341942040|sp|Q9R1B9.2|SLIT2_MOUSE2.179e-2723.32RecName: Full=Slit homolog 2 protein; Short=Slit-2... [more]

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BLAST of hypothetical protein DAPPUDRAFT_300683 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR)
Total hits: 25
Match NameE-valueIdentityDescription
gi|1325301348|ref|XP_023332215.1|2.192e-3851.38chaoptin-like, partial [Eurytemora affinis][more]
gi|321458747|gb|EFX69810.1|4.330e-4339.05hypothetical protein DAPPUDRAFT_300683 [Daphnia pu... [more]
gi|1067081576|ref|XP_018024567.1|1.233e-3839.12PREDICTED: chaoptin-like [Hyalella azteca][more]
gi|1022762791|gb|KZS08454.1|1.994e-17539.90Chaoptin [Daphnia magna][more]
gi|970902791|ref|XP_015117217.1|2.014e-16737.18PREDICTED: chaoptin [Diachasma alloeum][more]
gi|1068414380|ref|XP_018053036.1|2.676e-16137.30PREDICTED: chaoptin [Atta colombica][more]
gi|780685750|ref|XP_011698502.1|3.511e-16137.25PREDICTED: chaoptin isoform X3 [Wasmannia auropunc... [more]
gi|1059881407|ref|XP_017796115.1|3.902e-16137.35PREDICTED: chaoptin [Habropoda laboriosa][more]
gi|332022695|gb|EGI62972.1|9.956e-16137.19Chaoptin [Acromyrmex echinatior][more]
gi|1061479428|gb|ODM96941.1|1.075e-16037.22Chaoptin [Orchesella cincta][more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
scaffold1086_size63525supercontigscaffold1086_size63525:3519..6759 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
maker2018-02-12 .496401
T. kinsejongensis vs L. Salmonis peptides2018-04-19
T. kingejongensis peptided Blastp vs. SwissProt2018-04-19
T. kingsejongensis proteins Blastp vs. NR2018-05-15
Properties
Property NameValue
Note-
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
snap_masked-scaffold1086_size63525-processed-gene-0.0-mRNA-1snap_masked-scaffold1086_size63525-processed-gene-0.0-mRNA-1Tigriopus kingsejongensismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at scaffold1086_size63525:3519..6759+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>snap_masked-scaffold1086_size63525-processed-gene-0.0 ID=snap_masked-scaffold1086_size63525-processed-gene-0.0|Name=hypothetical protein DAPPUDRAFT_300683|organism=Tigriopus kingsejongensis|type=gene|length=3241bp|location=Sequence derived from alignment at scaffold1086_size63525:3519..6759+ (Tigriopus kingsejongensis)
ATGGCTGAGAACGTCGAGCTCTCTATCCACTGTTCTTTGCACTCCAAGGA CGAAAAACAAGCTCTTTATCATCATCCAGCAGCCGAGCTTTGGGACAGTG CTCAGTGGATATCCCTGGGTCTCTGGGTGGGCGGGAGAGGAGAGCGTTGT GCATTAGGGGGTGTTTTCGGGCGAACTGGAGCTCATTTGTCCTGTTTGTT TTCAGATAACCAGATTTCTGAATTGAGTCGAGAAGACTTGCGATCTCTGG CACTCTCCTTGCGCGAGCTCAATTTGGCCGGCAATGCGCTCACCTCGGTG CCTGAGGGCATCTTTTGGGATCTGCAACGCCTCGAGAGGCTCGATCTAAG TCGCAACAACATCATTCAAATCCACTCGATGGCATTTCAAACCGGGGTAG GTTGAACACTGCATGCACTAAGTGCTTTCGTTCCCTGCTGATGGGACACA TCGTCGTTCTCCAATGGAGCTCACGCTAATCTGCCTCTCCGACTTAGGTG CCCCATTTGGCCGTGCTGGATTTGGCGGATAATCTGATGGGGAAGATCCC GTTCATCTCCATAGCCCACGTGAAGTCGCTGCGCGTGTTGGATCTCTCCA ACAACCGGATTGAAAAAGTGGCGGATCCCTTCTTCATTGGCCAGCTCCAC CTGGACACACTGCAACTGCGGGAGAACAACATTCAAAATCTGACGGCCAA CGCCTTCCAGAACTTTGAGTACATCAATTACACGTCGCTCGCGGGCAATC CACTGGAGTGGATCGAACCCGACGCCTTCTTGGAGACGCAGATCAAGCTC CTGGATCTCTCCAATTGTCTCATCCAAGAGCTCGCCTCCAATGCGCTGCG AGGGCTGGAAAACAGTTTGGAGGCCTTGGATTTGAGTGCCAATCGACTCC AGGAGTTGCCCGAGGACATTTTCATGGATTTCGATACCATCCGACGGTTG CGACTCAACGACAACATGCTCCAATTCTCACCCAACATCACCTTCAACGG CTTCCGCTACACCATCAAGGACTTGAACCTGCTGGGCCAAGACATGTACT ACGTGCCCCTCGAGGAGATTGGGATCATGCGGAACCTGCGCACCGTGGGC CTGTCCTCGGTCAATAACTATGGCTCGGTGACGACGGATCAGTTCGCAGA CTTGTCGCCCTCCTTGGAGGCTCTGAACCTGGTGGACTCGGACCTGACCT CCATTGAGCACAACGCCTTCAAGCATGTGCCCAGCATCTCCTCCATGGAT CTCTCGAACAATCGCATCGAGTACATCGAGGAGAATGCCTTTCGCGAGGT GGGCAATGCCCTTCAATACCTCCGCATGTCGAATGCTCTCCACTTCCGAG CGCTGCCCAATGGGCCCTTCCGCAGCTTGAGTGCACTCCGCACACTCGAC CTGAGCAATAACCATATCCGAAGTTTGCCACTTGACACCTTTCACAAGAT GACTCGCCTCCAACACTTGTACCTGCAAGTAAGTACCCCAGCCTCCCCGC TTCCCTGCCCGTGCATGCCAGGCAGTGTGCACTCGAGGCACGTTGCCATA GCAGTAGATGCCAACGTCTCTCGGTCCCGACTAGTCTTGGTAGGACCGAT CGTTTCGTTTTCATTAACTCTCACTCTCGGGACTGGTTTTTTTGTCCCTC GTGCCATTTCCACCCACGATTCGCTAGGTAGTGGGCGAACAGCAAACATC CTCATCCTCGAATGAGTGGCTGTGTCCCGCTCTACCGTATGTAGCATCCC AAGCCGGTCTGATCTCTCGCTCTCACACACTATTTCTTCTCTCTTTCCGA ACGTGCGCAGGACAACGAGATCTCAACTTTCAAACGAGGCACTTTCCACA GTCAGGCCAACCCCAGCCTGGCCATCCTGGATCTGAGCTTTAATCACATC GAGAGAGTGGAATATGACACTTTTCGCTTTGGCAAACTCCAACAATTGCT CCTCAACGACAACCGAATCAAGAAATTGGATTCCCAGAGCTTCGTCGAGA TGACACGCTTGCTCATCCTCTCGCTGGAAGGAAATCAAATCCGACAGCTG CCAGATGAGACCTTTCAAAACCTTCACCGATTGCAGCGCCTCGACCTGGC CTATAACCGCTTGGAGTCGCTCAATTTTGCAGCCTTCGATTCCATTGGCA CCCTGTCGCACCTCGCGATTGATTTGAGCCACAACCAACTCCAGGCCTTG CGGGTCAATCGGAGCAACACCTATCCCACCCTGTCGAACATCATGAGCCT GGACTTGAGCTACAACAACATCTCCTTTGTCGAGGTCACCTTCTTCGAGC CCGTGCAGAATGATTTGAAAATTTTGAACCTTTCCCGCAACATCATCAGC GAGATCACGCCGGACGACGTGGGCCAATTGCGGCGGATCCGCGTTTTGGA CCTCAGTCATAACTGCATCTCCCACATTGAAGATCCGACCTTTGGGGCGG CCAACAAATTGTCGAGTCTCTACTTGAATCACAACCGTCTGACGAGCTTG CACGGAAAGTTGCTGCGAAATCAAAAGCGATTGCAAGTCGTGGACTTGTC CCATAACTTCTTGGAAACTCTTCCCGAAGAATTGTTTCTGCGCACTTCCT TGGAAATATTCAAAGTTGCCCACAATCGATTGTACGAGATCCCGGTGAAG GCACTCAACCCGGTGCAAAGCTCCCTAAAATATCTGGATTTATCCGCTAA TCAAATCACCACCATATCCGACTCGCAGCTCAACCAGATCCAGCTCCTGG TCTACCTCAATCTGGAGGGCAACAAGATCTCCACCATCGACGCAGATGCC TTCTGCTGTGTGCCCAATCTGGAGCAACTCAATCTGGCCAGCAATCCCCT TCATCGGATCCACCTCGACACGTTCGGCGGAATTAGGGACCATCTGCAGG GACTGAATGTGGCCAATACCTCGCTCACGCTCCTGCCCAATCTCCAATTA TCCAGCCTCAAGTCCCTTAATGTCTCTTTCAATCAATTGACTTTCATCCC ACCCAACACCTTGGCTAATTTATCTCAAGTCCGTGAATTGGATTTGAGTG GAAATGAGCTGACGAGCCCACCGAGCACGGCCTGGCACTCCATGGACCAC CTCCTCTCTCTCCACCTGTCAGGCAATCCCATCGTCCGCGTGATGAATGA CTCCTTCGCCGGACTCAGGCACTTGGAGAGCCTCGACATTTCTGGCGTGA GAGCCAAGTCATTTCAGGTACGTGCGAGTGACTGTTCGTAG
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Synonyms
The feature 'hypothetical protein DAPPUDRAFT_300683' has the following synonyms
Synonym
Tk01501
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