PREDICTED: neprilysin-2-like, snap_masked-scaffold243_size241480-processed-gene-1.14 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Match: EMLSAG00000009482 (supercontig:LSalAtl2s:LSalAtl2s611:16796:20138:-1 gene:EMLSAG00000009482 transcript:EMLSAT00000009482 description:"maker-LSalAtl2s611-augustus-gene-0.6") HSP 1 Score: 416.772 bits (1070), Expect = 1.370e-128 Identity = 224/702 (31.91%), Postives = 371/702 (52.85%), Query Frame = 0
Query: 23 CLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTL-------------GGWPVL---------------------------------------------EENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTH--NITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA--------------------------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
C+S+ C+ A+ + ++M+ S+DPCE+FY F+CG ++ T+IP+D++ S ++L+ +L + ++LLE E ++ F+ + Y +C+N+++++ + +P+ +LD L GG P + E LI+G D+ V+AY+++M D+ GA+ N + ++K+VL EM+LA S P+E RR+ KLYNP +KD L P W +++N++ + + E +++ P + K S ++ S R ANY+ WR V + + DA+ +L++SK +G RW+TCV A + S A G++Y + YF +E++ E+++++R F +I+N I+WMD TK A +K ++ IGYP E+L+++K+ Y+G+ + + Y + L++ + + LRE+++K W G A+VNAFY +N I FPAG+L+G FF+ P Y+NYGA+G T +++ +KA+CII+Q NY ++ + LNGINTQGENIADNGG+ +AY+A+ + +N + +LPGL ++ +QLFW+S VWC K R + LK +L H+P+ FRV G S EFARDF CP GS MNP ++C
Sbjct: 97 CMSKGCITTASEILRNMDESVDPCEDFYRFSCGGFIDKTIIPDDRTRMSSFSVLSDELLAQVRYLLESESLANEPRPFRMAKDVYNACMNKEQIEVLGQDPIKNILDRLGGWPVIIGDSWNNGGKPYVWYKQVYMLRRIGYSVDYLVDFSVAIDLKNSSRRILDLDQPRFVLDREYLIKGFNDEDVQAYYEFMMDVARFFGANENRAAKDMKEVLELEMKLANFSLPRENRRNATKLYNPMTIKDLTKLDPFTP-WLDYINNILTKEIIQVNDKEVIIVNVPSYIKNFSLLINNTSDRVKANYLLWRVVSASIKYLNDDAKKISLQFSKKLTGKTEETPRWRTCVDGA------KRSLTNAVGAMYIRKYFKEESRKSACEMVKDIRIEFDKILNQIDWMDAKTKAMAKKKANSIVDHIGYPPELLDDNKLEELYDGLQLGNLHYLENALNMTVFVANYAFSLLREKVNKTDWVRHGKPAIVNAFYSPLENSIQFPAGILQGIFFSSDRPRYMNYGAIGWVIGHEITHGFDDQGRQFDLEGIFIFFKNIDNIPFFLGNLVDWWHPTTKEQYLKKAKCIIEQYGNYFLPELNITLNGINTQGENIADNGGIKEAYRAYVNWVDRNEEEKLLPGLNYSQKQLFWVSAANVWCAKFRPKALKLRVLTGVHSPDMFRVRGPFSNMPEFARDFNCPKGSHMNPAKRC 791 HSP 2 Score: 374.785 bits (961), Expect = 3.037e-113 Identity = 192/479 (40.08%), Postives = 284/479 (59.29%), Query Frame = 0
Query: 894 GYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTH--NITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQN----------------GDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAYA 1354
GYS+D +VD + D KNS+ R + LDQP L REYLI+G D+ V+AY+++M D+ GA+ N + ++K+VL EM+LA S P+E RR+ KLYNP +KD L P W +++N++ + + E +++ P + K S ++ S R ANY+ WR V + + DA+ +L+++K +G E RW TCV ++ S G++Y + YF +E++ +++++R F +IL+ IDWMD TK A +K ++ IG+P E+L+++K+ +Y G+Q+ + +N L++ + + LRE+V+K W A+VNA YSP +N I FPAGIL G FF++D P Y NYGAIG VIGHEITHGFDDQGR+ D G+L DWW T +++ KA+CII Q N+ ++ + LNGINTQGENIADNGGI EAYRAY
Sbjct: 236 GYSVDYLVDFSVAIDLKNSSRRILDLDQPRFVLDREYLIKGFNDEDVQAYYEFMMDVARFFGANENRAAKDMKEVLELEMKLANFSLPRENRRNATKLYNPMTIKDLTKLDPFTP-WLDYINNILTKEIIQVNDKEVIIVNVPSYIKNFSLLINNTSDRVKANYLLWRVVSASIKYLNDDAKKISLQFSKKLTGKTEETPRWRTCVDG------AKRSLTNAVGAMYIRKYFKEESRKSACEMVKDIRIEFDKILNQIDWMDAKTKAMAKKKANSIVDHIGYPPELLDDNKLEELYDGLQLGNLHYLENALNMTVFVANYAFSLLREKVNKTDWVRHGKPAIVNAFYSPLENSIQFPAGILQGIFFSSDRPRYMNYGAIGWVIGHEITHGFDDQGRQFDLEGIFIFFKNIDNIPFFLGNLVDWWHPTTKEQYLKKAKCIIEQYGNYFLPELNITLNGINTQGENIADNGGIKEAYRAYV 707 HSP 3 Score: 227.254 bits (578), Expect = 4.042e-62 Identity = 105/296 (35.47%), Postives = 178/296 (60.14%), Query Frame = 0
Query: 600 KEFARDFQCPIGSPMNPEEKCRSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDR--TDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTH--NITEDERVLIGNPEFFKKLS 891
K +D+ G E R+M+ S+DPCE+FY F+CG ++ T+IP+D++ S ++L+ +L + ++LLE E ++ F+ + Y +C+N+++++ + +P+ +LD LGGWPV+ G +W+ Y WY+ +Y L + GYS+D +VD + D KNS+ R + LDQP L REYLI+G D+ V+AY+++M D+ GA+ N + ++K+VL EM+LA S P+E RR+ KLYNP +KD L P W +++N++ + + E +++ P + K S
Sbjct: 90 KASIQDYCMSKGCITTASEILRNMDESVDPCEDFYRFSCGGFIDKTIIPDDRTRMSSFSVLSDELLAQVRYLLESESLANEPRPFRMAKDVYNACMNKEQIEVLGQDPIKNILDRLGGWPVIIGDSWNNGGKPYVWYKQVYMLRRIGYSVDYLVDFSVAIDLKNSSRRILDLDQPRFVLDREYLIKGFNDEDVQAYYEFMMDVARFFGANENRAAKDMKEVLELEMKLANFSLPRENRRNATKLYNPMTIKDLTKLDPFTP-WLDYINNILTKEIIQVNDKEVIIVNVPSYIKNFS 384
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Match: EMLSAG00000007047 (supercontig:LSalAtl2s:LSalAtl2s3:1196101:1219563:1 gene:EMLSAG00000007047 transcript:EMLSAT00000007047 description:"maker-LSalAtl2s3-snap-gene-12.11") HSP 1 Score: 413.305 bits (1061), Expect = 2.139e-128 Identity = 234/636 (36.79%), Postives = 346/636 (54.40%), Query Frame = 0
Query: 23 CLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTL------GGWPVLEENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAG-FGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCRSM 623
C + C+ A+ L Q+MN + I +DK+ S+ +I+ +L ++ + +LE G+++D +F +R Y++C+NED ++ + EP+ + L +L + E L++G E ++V Y+ YMQ + L+GA + +LK+ LLFEM+LA S PKEERR+ +KLYNP L D VD+L+ N T+ + P+ R +ANY+ WR + + + AR+ +LEY K +GT A RWK CV AAG GT G LY YF ++AK M E+++ +++ F +I+ +I WMD+ T+ +AI KL A+ + I YP+EI++ + + Y+G+ + + YF + SL S ++ +++LRE+IDK W A+VNAFY +N I FPAG+L+G FFN P Y+NYG++G +T KKF EKAQCII Q NY SK V V LNG+NTQGENIADNGGL +AY+A+ + +G + LPGL F RQLFW+S WC K R + LK IL H+P FRV G S KEF++DF+CP+G+ MNP KC +
Sbjct: 88 CTTPDCIASAHSLLQNMN------------------PESRIADDKTYKSQFSIIGDELDEQIRDILESNGTENDSLVFNQVRSIYQACMNEDSLEGLGLEPMLKELKSLVMGFDQASLGLSREYLVQGFESENVNYYYSYMQKVAVLLGASPEEAATQLKESLLFEMKLANASLPKEERRNASKLYNPMKLID--------------VDDLMPGVNFTD----YVNEPK--------------RNVANYLLWRIASQGLFVLNKAAREISLEYRKNVTGTPADAPRWKKCVGAAGSLGT--------VVGHLYVSKYFKEDAKHNMMEMVKYIKKEFKKILQEITWMDEQTRTRAINKLAAIKEYIAYPEEIMKVENLEELYKGLKINNNTYFENEKSLSLWSYSYLWKKLREKIDKTDWKRHAEPAVVNAFYSSIENSIQFPAGILQGVFFNKNRPHYMNYGSIGWVIGHEITHGFDDRGRQSDSNGNLNDWWEMETKKKFLEKAQCIIWQYGNYTSKAVNVSLNGVNTQGENIADNGGLKEAYRAYVSWESVHGQENSLPGLNFNSRQLFWLSAANNWCSKFRPKVLKNRILTGYHSPGAFRVRGTFSNLKEFSQDFKCPLGTNMNPINKCECI 665 HSP 2 Score: 368.237 bits (944), Expect = 7.015e-112 Identity = 191/436 (43.81%), Postives = 266/436 (61.01%), Query Frame = 0
Query: 919 LDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAYA 1354
DQ LGLSREYL++G E ++V Y+ YMQ + L+GA + +LK+ LLFEM+LA S PKEERR+ +KLYNP L D VD+L+ N T+ + P+ R +ANY+ WR + + + AR+ +LEY K +GT RW+ CV A G S G LY YF ++AK + +++ ++ FK+IL +I WMD+ T+ +A KLAA+ + I +P+EI++ + + +YKG++I +F+N SL S + +++LRE++DK W A A+VNA YS +N I FPAGIL G FFN + P Y NYG+IG VIGHEITHGFDD+GR++D NG+L DWW+ +T +KF +KAQCII Q N+ S V + LNG+NTQGENIADNGG+ EAYRAY
Sbjct: 182 FDQASLGLSREYLVQGFESENVNYYYSYMQKVAVLLGASPEEAATQLKESLLFEMKLANASLPKEERRNASKLYNPMKLID--------------VDDLMPGVNFTD----YVNEPK--------------RNVANYLLWRIASQGLFVLNKAAREISLEYRKNVTGTPADAPRWKKCVGAAG-------SLGTVVGHLYVSKYFKEDAKHNMMEMVKYIKKEFKKILQEITWMDEQTRTRAINKLAAIKEYIAYPEEIMKVENLEELYKGLKINNNTYFENEKSLSLWSYSYLWKKLREKIDKTDWKRHAEPAVVNAFYSSIENSIQFPAGILQGVFFNKNRPHYMNYGSIGWVIGHEITHGFDDRGRQSDSNGNLNDWWEMETKKKFLEKAQCIIWQYGNYTSKAVNVSLNGVNTQGENIADNGGLKEAYRAYV 578 HSP 3 Score: 117.087 bits (292), Expect = 8.952e-27 Identity = 67/210 (31.90%), Postives = 106/210 (50.48%), Query Frame = 0
Query: 647 IPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESL-PG 855
I +DK+ S+ +I+ +L ++ + +LE G+++D +F +R Y++C+NED ++ + EP+ + L +L + DQ LGLSREYL++G E ++V Y+ YMQ + L+GA + +LK+ LLFEM+LA S PKEERR+ +KLYNP L D + L PG
Sbjct: 110 IADDKTYKSQFSIIGDELDEQIRDILESNGTENDSLVFNQVRSIYQACMNEDSLEGLGLEPMLKELKSL--------------------------------------------------VMGFDQASLGLSREYLVQGFESENVNYYYSYMQKVAVLLGASPEEAATQLKESLLFEMKLANASLPKEERRNASKLYNPMKLIDVDDLMPG 269
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Match: EMLSAG00000006703 (supercontig:LSalAtl2s:LSalAtl2s372:146954:340151:1 gene:EMLSAG00000006703 transcript:EMLSAT00000006703 description:"maker-LSalAtl2s372-augustus-gene-3.13") HSP 1 Score: 349.747 bits (896), Expect = 9.594e-105 Identity = 203/684 (29.68%), Postives = 342/684 (50.00%), Query Frame = 0
Query: 21 KMCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPV------------------LEENLIEGL-------------------------------------EDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
K+CL++ CV + L +M+ + DPC F+++ CG VIPED+SS S ++ LQ K LLE++ ++ D E ++ FY+SC+N ++++I+ PL + ++ LGGWPV L++N G+ D+ +KAY YM ++ L+GA++ ++ + ++V+ FE +A IS P+ +R + +Y LKD ++ +P +W + D ++ I ++E+++ + +FK L ++ + + + NY+ WR V ++ E+ +V G RW CV A A G+L+ + F ++K I +I ++RE+F +++ + EWMD+ T+ A +K +M++ IGYP+ I + ++ YE + + + + ++ R + +LR +DK W A+VNAFY+ +KN I FPAG+L+ F++ P +NYG +G+ T +F AQCIIDQ + Y + + +NG TQGENIADNGGL QA++A+ K + NG + +LPGL T +QLF++++ ++WC R E KT I H+P RV G LS + +FA + CP GS MNPE+KC
Sbjct: 68 KICLTQECVQTSAYLLGAMDTTADPCTXFFQYACGAWNRKHVIPEDRSSISTFEVMADQLQIILKGLLEEDHNEFDCEATLKVKNFYKSCMNISQIERISDTPLVQAINGLGGWPVVDPYWTSHNWTIERVIGILKKNFTLGIFIEEWVGPDDRNSSAHIIQIDQMTFGLPSRDYFLLEESDRDLKAYHIYMTEVGALLGANKTYAYEQFQNVIRFEKSIANISVPEHDRINTGAIYTKISLKDLKTEVP--EINWNDYFDTVMGIGYIGDNEQIVSYSMPYFKLLGKLLKQTDKKIIHNYMIWRTVLELMPHLPPQYESTRAEFRRVLLGVLTGRIRWHKCVDWAN------KKMGMAVGALFIRDNFNYDSKEIALSMIHDIREAFNDLLEENEWMDEETRLVAKDKANSMNERIGYPEYITNKARLNQEYENLSITPVNFLDNLFNILRFEASNNQRKLRLAVDKDKWQTEP--AVVNAFYNPNKNDIVFPAGILQPLFYSQHFPKSLNYGGIGVVIGHEITHGFDDKGRQFDKFGNLKQWWNNRTIAEFHNAAQCIIDQYSTYMISDIQLNINGRMTQGENIADNGGLKQAFRAYNKWVRINGEEDLLPGLNLTHKQLFFLNYAQIWCGSMRPEDAKTKIRAAVHSPGPIRVLGPLSNSVDFAEAYSCPKGSRMNPEQKC 741 HSP 2 Score: 284.263 bits (726), Expect = 1.148e-81 Identity = 180/593 (30.35%), Postives = 309/593 (52.11%), Query Frame = 0
Query: 777 SREYLIEGLEDQHVKAYFKYMQD-----ITELMGADRNASEAE----LKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHP---PSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAYAK 1355
+R+++I ED+ + F+ M D + L+ D N + E +K+ M ++QI ++ + L++ L G P P WTS HN T ERV+ + + +++ + ++ ++ D +NS+ I +DQ GL SR+Y + D+ +KAY YM ++ L+GA++ + + ++V+ FE +A IS P+ +R + +Y LKD ++ +P +W + D ++ I ++E+++ + +FK L ++ + + + NY+ WR V ++ E+ +V G RW CV + G+L+ + F ++K +I ++R +F ++L++ +WMD+ T+ A +K +M++ IG+P+ I + ++ Y+ + I N+ ++ R +LR VDK W A+VNA Y+P KN I+FPAGIL F++ P NYG IGVVIGHEITHGFDD+GR+ D+ G+L WW+ T +F + AQCII+Q + + + +QL +NG TQGENIADNGG+ +A+RAY K
Sbjct: 105 NRKHVIP--EDRSSISTFEVMADQLQIILKGLLEEDHNEFDCEATLKVKNFYKSCMNISQIE----------RISDTPLVQAINGLGGWPVVDPYWTS--------HNWT-IERVI--------GILKKNFTLGIFIEEWVGPDDRNSSAHIIQIDQMTFGLPSRDYFLLEESDRDLKAYHIYMTEVGALLGANKTYAYEQFQNVIRFEKSIANISVPEHDRINTGAIYTKISLKDLKTEVP--EINWNDYFDTVMGIGYIGDNEQIVSYSMPYFKLLGKLLKQTDKKIIHNYMIWRTVLELMPHLPPQYESTRAEFRRVLLGVLTGRIRWHKCVDW------ANKKMGMAVGALFIRDNFNYDSKEIALSMIHDIREAFNDLLEENEWMDEETRLVAKDKANSMNERIGYPEYITNKARLNQEYENLSITPVNFLDNLFNILRFEASNNQRKLRLAVDKDKWQTEP--AVVNAFYNPNKNDIVFPAGILQPLFYSQHFPKSLNYGGIGVVIGHEITHGFDDKGRQFDKFGNLKQWWNNRTIAEFHNAAQCIIDQYSTYMISDIQLNINGRMTQGENIADNGGLKQAFRAYNK 658 HSP 3 Score: 145.976 bits (367), Expect = 7.992e-36 Identity = 82/272 (30.15%), Postives = 156/272 (57.35%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKW-YENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKL 890
+M+ + DPC F+++ CG VIPED+SS S ++ LQ K LLE++ ++ D E ++ FY+SC+N ++++I+ PL + ++ LGGWPV++ W T + W E + + K+ +++ + ++ + D +NS+ I +DQ GL SR+Y + D+ +KAY YM ++ L+GA++ + + ++V+ FE +A IS P+ +R + +Y LKD ++ +P +W + D ++ I ++E+++ + +FK L
Sbjct: 85 AMDTTADPCTXFFQYACGAWNRKHVIPEDRSSISTFEVMADQLQIILKGLLEEDHNEFDCEATLKVKNFYKSCMNISQIERISDTPLVQAINGLGGWPVVD-PYW--TSHNWTIERVIGILKKNFTLGIFIEEWVGPDDRNSSAHIIQIDQMTFGLPSRDYFLLEESDRDLKAYHIYMTEVGALLGANKTYAYEQFQNVIRFEKSIANISVPEHDRINTGAIYTKISLKDLKTEVP--EINWNDYFDTVMGIGYIGDNEQIVSYSMPYFKLL 351
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Match: EMLSAG00000002013 (supercontig:LSalAtl2s:LSalAtl2s135:683680:686597:-1 gene:EMLSAG00000002013 transcript:EMLSAT00000002013 description:"maker-LSalAtl2s135-snap-gene-6.23") HSP 1 Score: 271.552 bits (693), Expect = 1.764e-78 Identity = 181/635 (28.50%), Postives = 296/635 (46.61%), Query Frame = 0
Query: 21 KMCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITE---DERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPM--LPGLP-FTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCR 621
K C ++AC A L ++N S++PCE+FY++ CG V TVIP + + L + L ++ K +LE S ++ F R FY+SC+NE +++ +PL ++L LGGW L H ++ +I+ + RN ++ E K + LYN +K+ +L P W ++ N+ T + + + +++ P + + L +VM S + + N++ W+ V++ + ++A +Y GT R CV A + A G++Y + +F +K + E+I N RE F ++I + +WM + TK A+EK +AM +GYP E L+ D +T RY G+ + YF + + F + L + ++K +W G A+VNA+Y +N + FPAG+L+G FF+ P Y+N G +G T++ +EEK +C+ Q NY + V V ++G T ENIADN G+ AY F K + LPG +T Q FW+S +C K + K +AP +R+ G+ S EF+ DF C GS MN EKC+
Sbjct: 24 KYCTTQACNDTAKKLLLNINPSINPCEDFYQYACGGFVNRTVIPPGRKGIDIASELTRKLNKQLKTVLEANSSSNEPGAFITARNFYKSCVNESQIEVQGLQPLKDILSKLGGW----------LNSTHNES------NISGIKYDWRNDANLEQKSIA--------------------DLYNKMSIKNLSNLNPCVP-WLDYI-NIFLTKELIQVNGSDEIIVTEPYYVRNLYNVMKNTSLKVVNNFVRWKIVQNSIPYLNKEASIIREKYISKIYGTSGPIDRSLICVEEAN------KNLQNAVGAMYVRSFFKNGSKEDVEEMIGNTREVFYQMIIESDWMTNGTKASALEKALAMFNHVGYPAEYLDNDTLTERYNGLELNSLDYFGNKHRIWTFDLDFSIKSLNKRVNKTSWIYNGESAVVNAYYSTDENSLIFPAGMLQGMFFSVDRPTYLNLGGIGWIIGHEISHGFDNVGSQIDKDGNLFNWWDNVTAQIYEEKIKCLKYQYGNYTVESVNVTVDGEYTLEENIADNMGIRNAYLTFLKWKTSQTERNIEYLPGFDNYTINQAFWLSAANDYCIKMTSKTEKQRAETLPYAPEIYRIIGSFSNQPEFSSDFNCLAGSKMNRTEKCK 614 HSP 2 Score: 217.238 bits (552), Expect = 4.907e-60 Identity = 119/375 (31.73%), Postives = 200/375 (53.33%), Query Frame = 0
Query: 984 ERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITE---DERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAYAK 1355
E++ LYN +K+ +L P W ++ N+ T + + + +++ P + + L +VM S + + N++ W+ V++ + ++A +Y GT+ R CV+ + + G++Y + +F +K ++ +I N R F +++ + DWM +GTK A EK AM +G+P E L+ D +T Y G+++ +F N + F + L + V+K +W A+VNA YS +N ++FPAG+L G FF+ D P Y N G IG +IGHEI+HGFD+ G + D++G+L +WWD TAQ +E+K +C+ Q N+ V + ++G T ENIADN GI AY + K
Sbjct: 161 EQKSIADLYNKMSIKNLSNLNPCVP-WLDYI-NIFLTKELIQVNGSDEIIVTEPYYVRNLYNVMKNTSLKVVNNFVRWKIVQNSIPYLNKEASIIREKYISKIYGTSGPIDRSLICVE------EANKNLQNAVGAMYVRSFFKNGSKEDVEEMIGNTREVFYQMIIESDWMTNGTKASALEKALAMFNHVGYPAEYLDNDTLTERYNGLELNSLDYFGNKHRIWTFDLDFSIKSLNKRVNKTSWIYNGESAVVNAYYSTDENSLIFPAGMLQGMFFSVDRPTYLNLGGIGWIIGHEISHGFDNVGSQIDKDGNLFNWWDNVTAQIYEEKIKCLKYQYGNYTVESVNVTVDGEYTLEENIADNMGIRNAYLTFLK 527 HSP 3 Score: 78.9518 bits (193), Expect = 5.223e-15 Identity = 35/97 (36.08%), Postives = 58/97 (59.79%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGW 718
++N S++PCE+FY++ CG V TVIP + + L + L ++ K +LE S ++ F R FY+SC+NE +++ +PL ++L LGGW
Sbjct: 41 NINPSINPCEDFYQYACGGFVNRTVIPPGRKGIDIASELTRKLNKQLKTVLEANSSSNEPGAFITARNFYKSCVNESQIEVQGLQPLKDILSKLGGW 137
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Match: EMLSAG00000001658 (supercontig:LSalAtl2s:LSalAtl2s128:1881693:1892323:1 gene:EMLSAG00000001658 transcript:EMLSAT00000001658 description:"maker-LSalAtl2s128-snap-gene-19.19") HSP 1 Score: 263.848 bits (673), Expect = 1.968e-78 Identity = 145/397 (36.52%), Postives = 219/397 (55.16%), Query Frame = 0
Query: 253 VMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGM-DPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
++ K R AN++ W + S + +AR +Y + +G + + RWKTCV ++S GS+Y + YFP++AK+ ++ +++ F +++ +W D+ TK KA++K+ AM + IGYP+EIL + YEG+ + + YF + SL + + + + ++ EI W +L +VNAFY+ N I PAG+L+GNFF+ P Y+N+GA+GMA T KF K +CII++ ++ ++ +NG+NT GENIADNGGL AY+A+ L + +P+LPGL FT +QLFWIS +WC K R + L+ I D H P FRV GA S +FA DFQCPIGSPMNP+ KC
Sbjct: 2 LIRKTPIRVQANFLIWSVISSSMSYLNEEARHIQFKYIQKLTGAKTMSERWKTCVGE------TKSRLGIHIGSMYVRQYFPEKAKTAAQSIVSYIKKEFEKLLRQNKWSDEETKAKALKKVKAMHENIGYPNEILNNTVIDILYEGLDLGESTYFENVHSLFKWTMDYVFLKIENEIKSYDWTDLVTPTVVNAFYNPLTNSINIPAGILQGNFFSLHRPNYLNFGAIGMAIGHEITHGFDDTGRQFNFEGDLFDWWNPSTKLKFINKTKCIIEEYGDFAYPKLNTTVNGVNTLGENIADNGGLKVAYRAYASWLNQTSFPEPLLPGLNFTEKQLFWISNANLWCTKIRGKTLEQKIRRDVHTPAMFRVLGAFSNMVKFAEDFQCPIGSPMNPKIKC 392 HSP 2 Score: 237.654 bits (605), Expect = 2.783e-69 Identity = 122/317 (38.49%), Postives = 185/317 (58.36%), Query Frame = 0
Query: 1043 VMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATG--FGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAYAKNL 1357
++ K R AN++ W + S + +AR +Y + +G + RW+TCV T G H GS+Y + YFP++AK + ++ ++ F+++L W D+ TK KA +K+ AM + IG+P+EIL + +Y+G+ + E +F+N+ SL + + + + ++ E+ W + T +VNA Y+P N I PAGIL G FF+ P Y N+GAIG+ IGHEITHGFDD GR+ + GDL DWW+ T KF +K +CII + +F ++ +NG+NT GENIADNGG+ AYRAYA L
Sbjct: 2 LIRKTPIRVQANFLIWSVISSSMSYLNEEARHIQFKYIQKLTGAKTMSERWKTCVGETKSRLGIH--------IGSMYVRQYFPEKAKTAAQSIVSYIKKEFEKLLRQNKWSDEETKAKALKKVKAMHENIGYPNEILNNTVIDILYEGLDLGESTYFENVHSLFKWTMDYVFLKIENEIKSYDWTDLVTPTVVNAFYNPLTNSINIPAGILQGNFFSLHRPNYLNFGAIGMAIGHEITHGFDDTGRQFNFEGDLFDWWNPSTKLKFINKTKCIIEEYGDFAYPKLNTTVNGVNTLGENIADNGGLKVAYRAYASWL 310
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Match: EMLSAG00000004915 (supercontig:LSalAtl2s:LSalAtl2s2594:1939:5295:-1 gene:EMLSAG00000004915 transcript:EMLSAT00000004915 description:"maker-LSalAtl2s2594-snap-gene-0.3") HSP 1 Score: 260.766 bits (665), Expect = 1.004e-74 Identity = 175/662 (26.44%), Postives = 308/662 (46.53%), Query Frame = 0
Query: 39 MNRSMDPCENFYEFTCGRLVENTVIPEDK---SSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGW----------------------PVLEENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP-SWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----DTSKKFEEKAQCII---DQAN-------------------------NYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKN---------------------GMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCR 621
MN S DPC++FY+F CG + TV+ D+ +++SEI+ + K + + +D + F +R YR+C+NE ++ EPL +++ LGGW +L EN +KAYF+YM+D L+G + +L+++L FE+++A ++ K+E++D + LYN +K+ +L +P W ++++ + T + ++ V++ P + K L +M +S + +Y WR ++S + A++ + +S GT + + TC+ + A G++Y + +F + +K + ++I ++R +I+ + W+ + TK A++K A+ + YP E L++ K+ Y+G+ + + ++K W G +VNAFY N + PA +L+G F+ P Y+N GA+G T + K +CI D+ N + K V ++G T GENIADNGG+ +AY+A+ + N G +LPGL FT QLFW+S +WC K R + + LI + H+ R++ + S N++F RDF P+ S MNP +KCR
Sbjct: 1 MNNSADPCDDFYQFACGGFLNKTVVKSDEIKVNTFSEISD-----RVTLKHYWKXXSTTEDTKPFVLVRNLYRACINETAIETAGLEPLQKMISKLGGWLDTKDIHDMLRKGKKYNWTTDLKLLRENXYSTNILLELKAYFQYMKDAAVLLGHNLTEVEGKLQNILDFEIKMANLTKSKDEKKDISVLYNKMQIKNLTTL--NPCIDWLDYINSFLKTTQVQNEDNVIVSEPSYVKNLYGLMFDSSLEVVKDYARWRVIESSIPNLNAAAQNISEAFSNNLYGTSSKQSIEITCIQLVS------NKLKHAVGAMYVRDFFKESSKKNIKDMIHSIRLELYKILANATWLGEETKKNALDKAHAIFVHVAYPSEYLDDTKLEELYKGLDL-------------------------DAVNKTDWVNNGKGVIVNAFYSGLNNALVLPAAILQGLVFSADRPNYLNLGAIGWIIGHEITHDQLTRKIKCIFLLWDRVNLSAISKSKTIIVFVFFHIYMLFKRHFFCYKSVNKTIDGELTLGENIADNGGIREAYQAYLEWKNNNTGSIEILPGLNFTIEWKNNNTGSIEILPGLNFTSNQLFWLSGANMWCTKIRTKSEEMLIKTNPHSFPIARLDFSFSNNEDFVRDFNRPVDSKMNPSKKCR 624 HSP 2 Score: 190.274 bits (482), Expect = 7.444e-51 Identity = 120/424 (28.30%), Postives = 207/424 (48.82%), Query Frame = 0
Query: 940 VKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP-SWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCII---------NQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
+KAYF+YM+D L+G + E +L+++L FE+++A ++ K+E++D + LYN +K+ +L +P W ++++ + T + ++ V++ P + K L +M +S + +Y WR ++S + A++ + ++ GT+ + TC++ N G++Y + +F + +K +K +I ++R +IL + W+ + TK A +K A+ + +P E L++ K+ +YKG+ + + V+K W +VNA YS N ++ PA IL G F+ D P Y N GAIG +IGHEITH DQ R + L WD I+ + + F V ++G T GENIADNGGI EAY+AY
Sbjct: 132 LKAYFQYMKDAAVLLGHNLTEVEGKLQNILDFEIKMANLTKSKDEKKDISVLYNKMQIKNLTTL--NPCIDWLDYINSFLKTTQVQNEDNVIVSEPSYVKNLYGLMFDSSLEVVKDYARWRVIESSIPNLNAAAQNISEAFSNNLYGTSSKQSIEITCIQLVS------NKLKHAVGAMYVRDFFKESSKKNIKDMIHSIRLELYKILANATWLGEETKKNALDKAHAIFVHVAYPSEYLDDTKLEELYKGLDL-------------------------DAVNKTDWVNNGKGVIVNAFYSGLNNALVLPAAILQGLVFSADRPNYLNLGAIGWIIGHEITH---DQLTRKIKCIFLL--WDRVNLSAISKSKTIIVFVFFHIYMLFKRHFFCYKSVNKTIDGELTLGENIADNGGIREAYQAY 517 HSP 3 Score: 113.62 bits (283), Expect = 7.134e-26 Identity = 80/325 (24.62%), Postives = 152/325 (46.77%), Query Frame = 0
Query: 623 MNRSMDPCENFYEFTCGRLVENTVIPEDK---SSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRT-----DYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP-SWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQ 938
MN S DPC++FY+F CG + TV+ D+ +++SEI+ + K + + +D + F +R YR+C+NE ++ EPL +++ LGGW L+ K+ Y W ++ L + YS +++++L KAYF+YM+D L+G + E +L+++L FE+++A ++ K+E++D + LYN +K+ +L +P W ++++ + T + ++ V++ P + K L Y + L + D + WR I P L + + + E +
Sbjct: 1 MNNSADPCDDFYQFACGGFLNKTVVKSDEIKVNTFSEISD-----RVTLKHYWKXXSTTEDTKPFVLVRNLYRACINETAIETAGLEPLQKMISKLGGW--LDTKDIHDMLRKGKKYNWTTDLKLLRENXYSTNILLEL-------------------------------------KAYFQYMKDAAVLLGHNLTEVEGKLQNILDFEIKMANLTKSKDEKKDISVLYNKMQIKNLTTL--NPCIDWLDYINSFLKTTQVQNEDNVIVSEPSYVKNL----YGLMFDSSLEVVKDY--ARWRVIESSIPNLNAAAQNISEAFSNN 273
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Match: EMLSAG00000000064 (supercontig:LSalAtl2s:LSalAtl2s100:449072:453177:1 gene:EMLSAG00000000064 transcript:EMLSAT00000000064 description:"maker-LSalAtl2s100-snap-gene-4.23") HSP 1 Score: 256.914 bits (655), Expect = 4.525e-73 Identity = 136/467 (29.12%), Postives = 240/467 (51.39%), Query Frame = 0
Query: 887 FKKLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
K L + GYS + +++ +I+ D N + R + +D P G+ R++L++G D V+AYFKYM+D +G + + L+ +L FE+++A ++ +ER + +LYN +K+ L P W ++++ + + +++ +++ P + L +M K S + + +Y+ WRF++S + + A++ + + SGTN R TC++ + G++Y + +F + +K +K ++ +VR F +IL++ +W+ + TK A EK AM + I +P E L + K+ +Y+ + ++ +F KN+ + PAG+L G FF+ D P Y N G +G +IGHEITHGFD GR+ ++G L +WWD T F +++QC + Q N+ V NG T ENIADNGGI AY+A+
Sbjct: 160 LKILREYGYSTNFIMEFHIFQDLNNHSKRILLIDTPGFGIYRDFLVKGRGDFEVEAYFKYMKDAAVFLGHNLTDVQENLQSILNFEIQMANLTKSIDERINITELYNRMQIKNLTQLNPCIP-WIQYINSFLKITQVQKEDEIIVYEPAYISGLYRLMNKTSLKVVKDYVRWRFIESSLPELNKAAQNISNAFNDELSGTNGQKERDITCIEIVS------DKLEHAVGAMYVRTFFKESSKENIKDMVESVRQVFYKILNNTNWVGEETKRTALEKAHAMFEHIAYPSEYLNDTKLEELYQELNLENLTYF-------------------------------------------KNLQILPAGVLQGLFFSADRPNYLNLGTMGWIIGHEITHGFDSNGRQYTKDGVLENWWDNQTVTNFLNRSQCFVEQYGNYSIKNVNQTNNGKRTLHENIADNGGIRAAYQAF 576 HSP 2 Score: 245.743 bits (626), Expect = 3.017e-69 Identity = 148/518 (28.57%), Postives = 248/518 (47.88%), Query Frame = 0
Query: 133 GWPVLEENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMD-PMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCR 621
G+ + + L++G D V+AYFKYM+D +G + L+ +L FE+++A ++ +ER + +LYN +K+ L P W ++++ + + +++ +++ P + L +M K S + + +Y+ WRF++S + + A++ + ++ SGT R TC+ A G++Y + +F + +K + +++E+VR+ F +I+N+ W+ + TK A+EK AM + I YP E L + K+ Y+ + +E+ YF KN+ PAGVL+G FF+ P Y+N G +G T F ++QC ++Q NY K V NG T ENIADNGG+ AY+AF + N +LPGL FT QLFW+S +WC K+ + ++L+L D H+ RV+ + S ++ FA DF CP SPMNP E+CR
Sbjct: 196 GFGIYRDFLVKGRGDFEVEAYFKYMKDAAVFLGHNLTDVQENLQSILNFEIQMANLTKSIDERINITELYNRMQIKNLTQLNPCIP-WIQYINSFLKITQVQKEDEIIVYEPAYISGLYRLMNKTSLKVVKDYVRWRFIESSLPELNKAAQNISNAFNDELSGTNGQKERDITCIEIVS------DKLEHAVGAMYVRTFFKESSKENIKDMVESVRQVFYKILNNTNWVGEETKRTALEKAHAMFEHIAYPSEYLNDTKLEELYQELNLENLTYF-------------------------------------------KNLQILPAGVLQGLFFSADRPNYLNLGTMGWIIGHEITHGFDSNGRQYTKDGVLENWWDNQTVTNFLNRSQCFVEQYGNYSIKNVNQTNNGKRTLHENIADNGGIRAAYQAFIEWKNNNTKSIELLPGLNFTSNQLFWLSGANMWCSKNLKKVEESLVLTDEHSLATARVDLSFSNHEGFALDFSCPNDSPMNPPERCR 663 HSP 3 Score: 166.007 bits (419), Expect = 1.421e-42 Identity = 94/333 (28.23%), Postives = 173/333 (51.95%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKN---WDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDIT 951
+MN+S+DPC++FY+F CG V TV +S + + + L+ + K LLE + +D + F +R FY++C+NE M+ + +PL E + +LGGW E K+ T Y W ++ L + GYS + +++ HI+ D N + R + +D P G+ R++L++G D V+AYFKYM+D +G + + L+ +L FE+++A ++ +ER + +LYN +K+ L P W ++++ + + +++ +++ P + L Y + L + D WR I P L + + + D+ + +DIT
Sbjct: 42 NMNKSVDPCDDFYQFACGGFVNKTVFRPGESRLNTFSEIRNLLKNQLKTLLEMNPTSNDIKPFVMVRNFYQACINETAMEMVGLKPLQETILSLGGWLDTEDKSDVSTKGTKYNWTSDLKILREYGYSTNFIMEFHIFQDLNNHSKRILLIDTPGFGIYRDFLVKGRGDFEVEAYFKYMKDAAVFLGHNLTDVQENLQSILNFEIQMANLTKSIDERINITELYNRMQIKNLTQLNPCIP-WIQYINSFLKITQVQKEDEIIVYEPAYISGL----YRLMNKTSLKVVKDY--VRWRFIESSLPELNKAAQNISNAFNDELSGTNGQKERDIT 367 HSP 4 Score: 92.8189 bits (229), Expect = 2.995e-19 Identity = 42/114 (36.84%), Postives = 69/114 (60.53%), Query Frame = 0
Query: 21 KMCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGW 134
K C++EAC A + +MN+S+DPC++FY+F CG V TV +S + + + L+ + K LLE + +D + F +R FY++C+NE M+ + +PL E + +LGGW
Sbjct: 25 KYCVTEACNDTAKTILFNMNKSVDPCDDFYQFACGGFVNKTVFRPGESRLNTFSEIRNLLKNQLKTLLEMNPTSNDIKPFVMVRNFYQACINETAMEMVGLKPLQETILSLGGW 138
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Match: EMLSAG00000006546 (supercontig:LSalAtl2s:LSalAtl2s358:80738:83514:1 gene:EMLSAG00000006546 transcript:EMLSAT00000006546 description:"maker-LSalAtl2s358-snap-gene-0.19") HSP 1 Score: 238.424 bits (607), Expect = 9.810e-66 Identity = 153/506 (30.24%), Postives = 253/506 (50.00%), Query Frame = 0
Query: 873 ITEDERVLIGNPEFFKK--LSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYLIEGLEDQH--VKAYFKYMQDITELMGADRNASEAELK-----------DVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHN--ITEDERVLIGNPEFFKKLSDVM-----TKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAYAK 1355
I D I N +F +K L Y I D NS+ I LDQ L L ++++ ++ E +KA M + L+ + ++ K D+ FE +A I+ +R+ N LYN L ++ + +W+ +V+ N I EDE V++ P++FK L+D++ T TL NY+ W+ ++ + ++ RD L K G RW +C+ T + G+++ + F ++K +G+I ++ +F L++ WMD TK +A +K ++ MIG+PD IL ++ + YKG+ + E +F+N L+ + ++L ++V+ W T VNA Y+P KN I+FP+G+L FF D P N+GA+GVV+GHE++H FDD+GR+ D+NG++ DWW T F++K C+ Q +++ ++G T GENIADNGG+ +YRAY K
Sbjct: 219 IVMDPSYNISNFDFQRKMYLLQNKYHFGGFFSWSIVEDESNSSQHVIQLDQGGLSLPTQDFYVKDTEGNKRVIKALEIMMTRLFRLLLQEEYPNDPITKEGEDNIKQTVLDITNFEKRIANITISTSQRKQENNLYNRMTLGQL-NVNYNFMNWSIYVNEAFSQINKTIPEDEAVVVNAPKYFKALTDLINEHKATDNGKMTLNNYMIWQAIRDMKNTLSKSYRDTDLILQKAMFGKEGHEERWRSCITDTD------AVIGYALGAMFVRKVFQGQSKTVAEGMINRIKEAFIANLNEAAWMDATTKARAVDKAHSITDMIGYPDYILNKNDLNKKYKGLNVSESDYFRNTLAHSQFVFVESLKKLFKKVNLTQW--SMTPPTVNAYYTPVKNQIVFPSGLLQNPFFALDYPKSLNFGAMGVVMGHELSHAFDDEGRKYDKNGNMVDWWLNSTTLNFKNKLTCMTQQYSHYTLHNES--VDGNITVGENIADNGGLKASYRAYTK 713 HSP 2 Score: 231.491 bits (589), Expect = 1.723e-63 Identity = 145/480 (30.21%), Postives = 242/480 (50.42%), Query Frame = 0
Query: 177 DVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHN--ITEDERVLIGNPEFFKKLSDVM-----TKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPM-LPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
D+ FE +A I+ +R+ N LYN L ++ + +W+ +V+ N I EDE V++ P++FK L+D++ T TL NY+ W+ ++ + ++ RD L K G + RW++C+ T +++ +A G+++ + F ++K++ +I ++E+F +N+ WMD TK +A++K ++ MIGYPD IL ++ + +Y+G+ V + YF + L+ + ++L ++++ W VNA+Y KN I FP+G+L+ FF P +N+GA+G+ T+ F+ K C+ Q ++Y V +G T GENIADNGGL +Y+A+ K L +N + + LPG+ RQLF+ VWC S E ++ D H+P +FRV G LS KEFA F C S MNP+E+C
Sbjct: 329 DITNFEKRIANITISTSQRKQENNLYNRMTLGQL-NVNYNFMNWSIYVNEAFSQINKTIPEDEAVVVNAPKYFKALTDLINEHKATDNGKMTLNNYMIWQAIRDMKNTLSKSYRDTDLILQKAMFGKEGHEERWRSCI------TDTDAVIGYALGAMFVRKVFQGQSKTVAEGMINRIKEAFIANLNEAAWMDATTKARAVDKAHSITDMIGYPDYILNKNDLNKKYKGLNVSESDYFRNTLAHSQFVFVESLKKLFKKVNLTQWSMTP--PTVNAYYTPVKNQIVFPSGLLQNPFFALDYPKSLNFGAMGVVMGHELSHAFDDEGRKYDKNGNMVDWWLNSTTLNFKNKLTCMTQQYSHYTLHNESV--DGNITVGENIADNGGLKASYRAYTKWLEENTIPEVRLPGIKLNSRQLFF-----VWCSSSTKETDHFQLMEDHHSPGKFRVLGPLSNFKEFANVFLCSPYSKMNPKERC 792 HSP 3 Score: 93.9745 bits (232), Expect = 1.418e-19 Identity = 81/290 (27.93%), Postives = 144/290 (49.66%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGK-FLLEKEGSQDDWEIFKDMRKFYRSCLNE-DEMDKIAYEPLTEVLDT-LGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYLIEGLEDQH--VKAYFKYMQDITELMGADRNASEAELK-----------DVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHN--ITEDERVLIGNPEFFKKLSD 892
SM++ +DPCE+FY+F CG+ + IP+ KSSWS L++ Q K L EK +Q K R FY SC +E +++++ EPL ++++ + GW V++ +++ +++ + +Y L + Y I D NS+ I LDQ L L ++++ ++ E +KA M + L+ + ++ K D+ FE +A I+ +R+ N LYN L ++ + +W+ +V+ N I EDE V++ P++FK L+D
Sbjct: 120 SMDQDVDPCEDFYKFACGKWELSNPIPDGKSSWSPFEKLHEANQLVLKNILFEKNNTQISGAEEKARRYFY-SCFDEGGKIEEVKGEPLRKIINNDMEGWIVMD-PSYNISNFDFQRKMYLLQNK-YHFGGFFSWSIVEDESNSSQHVIQLDQGGLSLPTQDFYVKDTEGNKRVIKALEIMMTRLFRLLLQEEYPNDPITKEGEDNIKQTVLDITNFEKRIANITISTSQRKQENNLYNRMTLGQL-NVNYNFMNWSIYVNEAFSQINKTIPEDEAVVVNAPKYFKALTD 405 HSP 4 Score: 72.0182 bits (175), Expect = 8.855e-13 Identity = 41/120 (34.17%), Postives = 71/120 (59.17%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGK-FLLEKEGSQDDWEIFKDMRKFYRSCLNE-DEMDKIAYEPLTEVLDT-LGGWPVLE 138
+CL+ C+ ++ + SM++ +DPCE+FY+F CG+ + IP+ KSSWS L++ Q K L EK +Q K R FY SC +E +++++ EPL ++++ + GW V++
Sbjct: 104 VCLTPDCIQISASILSSMDQDVDPCEDFYKFACGKWELSNPIPDGKSSWSPFEKLHEANQLVLKNILFEKNNTQISGAEEKARRYFY-SCFDEGGKIEEVKGEPLRKIINNDMEGWIVMD 222
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Match: EMLSAG00000000565 (supercontig:LSalAtl2s:LSalAtl2s1093:286030:293067:1 gene:EMLSAG00000000565 transcript:EMLSAT00000000565 description:"maker-LSalAtl2s1093-snap-gene-1.20") HSP 1 Score: 216.083 bits (549), Expect = 9.693e-59 Identity = 172/689 (24.96%), Postives = 307/689 (44.56%), Query Frame = 0
Query: 23 CLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDW-EIFKDMRKFYRSCLNEDEMD---KIAYEPLTEVLDTLGGWPVL-----------------------EENLIEG---LEDQHVKAYFKYMQDIT---------------ELMGADRN--ASHAE-------------------LKDVLLFEMELAQISAPKEERRDRNKLYNPT----LLKDFESLPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAP-------------------AYVNYGAVGM--ADTS-KKFEEKAQCIIDQANNYESKQVG--VKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGL-PFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMN 615
C +E C A + M+ ++ PCE+FYEFTCG+ V++ P+ +S S A L+ D+ +L E + DD E+ K R +Y+SC+N ++ K Y+ + LGG+P+L E LI+ L+ Q+ + YM + E++ A R +S AE L+ + FE +A I P+++RR+ +YNP ++K + + W + + L+ N I E++++++ P +F+KL++++ + + + NYI WR + + + R+ + K G + R C FA Y + F ++K+ + +++ ++ SF E++ + WMD T+ KA EK+ +M Q +GYPD + E ++ +Y+ + +E+ K E I NA + FPAG+L+ FF P Y N G + +++S + F+++ +CII+Q + + ++ +NG+ TQGEN D GGL ++++A++K + N +P LP L +T Q+F+I++ + WC+ L+ IL D H+P +FR G +S +K F++ F C PMN
Sbjct: 81 CTTEVCKTSAKSIISKMDVNVSPCEDFYEFTCGKYVKDNTNPDGQSDXSIFADLSNDIMTTTLEIL--EANDDDVPEVVKKARSYYKSCINMSSINDEGKKQYKTIFGSKGALGGYPILNSQWNLEEKSKELALGRVLYDYNEGILIQSYVYLDAQNTAQHILYMDQASLGLLRINFVDSEKYKEIIKAYRTLQSSTAETLFKYLDINKPDDDKLNEDLESMFQFEKAIAGIMVPEDQRRNSTAMYNPMSLAKIMKSYTQI-----KWKIYFNELLKGDNAIEENDKIIVAEPYYFEKLNELLNETDDKIIYNYIHWRILLQTLPNGPDEMREHYKTFLKDAMGIKKEVLRDNICAKRVAAPFDGMGGLGFAVAYEYIQKKFDDDSKNEVKKMVGGLKSSFKELVAESSWMDKETQNKAKEKVDSMVQSLGYPDWLKTESEIEKKYKEL---------------------------DELKPKTLLE-NIKKYNNA------AAVTFPAGILQYPFFESSNPIIGVVIGHEITHGFDDQGSQYDNNGNLVKWWSNSSLEAFQKEKECIIEQYSAFNVPEISEETYVNGVLTQGENXXDXGGLRESFRAYKKWVNSNNDEPKLPELEKYTSEQMFFIAYSQTWCQVKTKASLQNQILSDPHSPGKFRSWGPVSNSKSFSKAFNCKPSDPMN 728 HSP 2 Score: 207.223 bits (526), Expect = 9.480e-56 Identity = 130/477 (27.25%), Postives = 232/477 (48.64%), Query Frame = 0
Query: 895 YSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDIT--------ELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPT----LLKDFESLPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGV-QIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQV--QLKLNGINTQGENIADNGGISEAYRAYAK 1355
Y+ +++ Y+Y DA+N+ +++DQ LGL R ++ + + + ++ +Q T ++ D + +L+ + FE +A I P+++RR+ +YNP ++K + + W + + L+ N I E++++++ P +F+KL++++ + + + NYI WR + + + R+ + K G + R C K F Y + F ++K +K ++ +++SFKE++ + WMD T+ KA EK+ +M Q +G+PD + E ++ YK + +++ +NI K ++ A V FPAGIL FF + P IGVVIGHEITHGFDDQG + D NG+L WW + + F+ + +CII Q + F ++ + +NG+ TQGEN D GG+ E++RAY K
Sbjct: 219 YNEGILIQSYVYLDAQNTAQHILYMDQASLGLLRINFVDSEKYKEIIKAYRTLQSSTAETLFKYLDINKPDDDKLNEDLESMFQFEKAIAGIMVPEDQRRNSTAMYNPMSLAKIMKSYTQI-----KWKIYFNELLKGDNAIEENDKIIVAEPYYFEKLNELLNETDDKIIYNYIHWRILLQTLPNGPDEMREHYKTFLKDAMGIKKEVLRDNICAKRVAAPFDGMGGLGFAVAYEYIQKKFDDDSKNEVKKMVGGLKSSFKELVAESSWMDKETQNKAKEKVDSMVQSLGYPDWLKTESEIEKKYKELDELKPKTLLENI---------------------KKYNNAAAVT--------------FPAGILQYPFFESSNP------IIGVVIGHEITHGFDDQGSQYDNNGNLVKWWSNSSLEAFQKEKECIIEQYSAFNVPEISEETYVNGVLTQGENXXDXGGLRESFRAYKK 649 HSP 3 Score: 107.457 bits (267), Expect = 7.996e-24 Identity = 74/290 (25.52%), Postives = 149/290 (51.38%), Query Frame = 0
Query: 623 MNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDW-EIFKDMRKFYRSCLNEDEMD---KIAYEPLTEVLDTLGGWPVLEGKNWD---RTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDIT--------ELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPT----LLKDFESLPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSD 892
M+ ++ PCE+FYEFTCG+ V++ P+ +S S A L+ D+ +L E + DD E+ K R +Y+SC+N ++ K Y+ + LGG+P+L + W+ ++ + Y EG +++ ++Y DA+N+ +++DQ LGL R ++ + + + ++ +Q T ++ D + +L+ + FE +A I P+++RR+ +YNP ++K + + W + + L+ N I E++++++ P +F+KL++
Sbjct: 97 MDVNVSPCEDFYEFTCGKYVKDNTNPDGQSDXSIFADLSNDIMTTTLEIL--EANDDDVPEVVKKARSYYKSCINMSSINDEGKKQYKTIFGSKGALGGYPILNSQ-WNLEEKSKELALGRVLYDYNEG----ILIQSYVYLDAQNTAQHILYMDQASLGLLRINFVDSEKYKEIIKAYRTLQSSTAETLFKYLDINKPDDDKLNEDLESMFQFEKAIAGIMVPEDQRRNSTAMYNPMSLAKIMKSYTQI-----KWKIYFNELLKGDNAIEENDKIIVAEPYYFEKLNE 374
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Match: EMLSAG00000009818 (supercontig:LSalAtl2s:LSalAtl2s639:492787:496659:1 gene:EMLSAG00000009818 transcript:EMLSAT00000009818 description:"maker-LSalAtl2s639-augustus-gene-5.12") HSP 1 Score: 213.001 bits (541), Expect = 5.535e-58 Identity = 136/442 (30.77%), Postives = 218/442 (49.32%), Query Frame = 0
Query: 889 KLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNI--TEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYF---PKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQ-IQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESA 1324
KL G+S ++ + D KN R I LDQP +G S+ L G + + Y +YM ++ L+GA + E+ V F+ L IS D+ +L +KD L G W S+++ ++ +I ++E V++ + +KL +++ K R ANY+ WR V+ + ++A+D A + G R + C K S G S+Y + Y PK+ + L ++N + + L ++ WMDD TK +A K+ M +IG+P E+L + ++ Y+G+Q +++ +HF N+LSL S+ Y LR V K W + T+A+ N Y N AGI+ G + D P Y N+ + + GHEITH FD GR ++ G+ +WW T FEDK QC+INQ NN+E +
Sbjct: 168 KLRKLGFSAKYFLNFLVETDIKNPNKRIIMLDQPYVGFSKFLLQFG--NDGIIEYIQYMVNMAXLLGATEEKARKEMLQVFEFQKSLMNISIKDPNLTDQYELMK---VKDLRKLDG-CIDWQSYINGMISNSSIFVEKEEEVVVYGRSYIEKLKNLILKTPIRVQANYMLWRVVEDSLLYLNKEAQDIANNFVTPVFGYAPETDRTKVCYKIV------EESIGIGMSSMYIRRYLAKIPKDLSVMLDTYLKN---EYLKSLSEVSWMDDETKKRAEVKMRKMKILIGYPPELLSDVEMKKYYQGLQSLEKDKHFTNMLSLMHYSKTKDYSLLRVPVTKNHWTKSGTIAIGNGAYRSLVNAYEIYAGIIRGIIYAEDRPKYINFALLAFIAGHEITHAFDVHGRTINEKGEEENWWSNATLTMFEDKKQCLINQYNNYEHS 594 HSP 2 Score: 195.282 bits (495), Expect = 4.713e-52 Identity = 138/500 (27.60%), Postives = 224/500 (44.80%), Query Frame = 0
Query: 153 YFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNI--TEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGV-VVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCR 621
Y +YM ++ L+GA + E+ V F+ L IS D+ +L +KD L G W S+++ ++ +I ++E V++ + +KL +++ K R ANY+ WR V+ + ++A+D A + G R K C E S S+Y + Y K K + L ++ + + ++++ WMDD TK +A K+ M +IGYP E+L + ++ Y+G+ +E K+F + LSL S+T Y LR + K W + G +A+ N Y N AG++RG + P Y+N+ + T FE+K QC+I+Q NNYE +D+ L + + + +LPGL +T QL+WIS +WC EH+ I D H+PN++RV G LS EF++ F C +PMN EKC+
Sbjct: 220 YIQYMVNMAXLLGATEEKARKEMLQVFEFQKSLMNISIKDPNLTDQYELMK---VKDLRKLDG-CIDWQSYINGMISNSSIFVEKEEEVVVYGRSYIEKLKNLILKTPIRVQANYMLWRVVEDSLLYLNKEAQDIANNFVTPVFGYAPETDRTKVCYKIV------EESIGIGMSSMYIRRYLAKIPKDLSVMLDTYLKNEYLKSLSEVSWMDDETKKRAEVKMRKMKILIGYPPELLSDVEMKKYYQGLQSLEKDKHFTNMLSLMHYSKTKDYSLLRVPVTKNHWTKSGTIAIGNGAYRSLVNAYEIYAGIIRGIIYAEDRPKYINFALLAFIAGHEITHAFDVHGRTINEKGEEENWWSNATLTMFEDKKQCLINQYNNYEH-----------------SDDTPLNGEFLLISAIKNDDHDEDLLPGLNYTQEQLYWISSANLWCSVYNPEHIFNEI-FDDHSPNKYRVIGPLSNRPEFSKAFHCAANTPMNRTEKCQ 691 HSP 3 Score: 138.658 bits (348), Expect = 1.145e-33 Identity = 74/266 (27.82%), Postives = 138/266 (51.88%), Query Frame = 0
Query: 610 IGSPMNPEEKCRSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWD--RTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNI 873
IG + +M+ +++PC++FY F+CG + T++P + + + L + L+ +E + + +IFKD++ FY++C++E++++++ P+ ++L +GGWPV+E +W R Y+WY L K G+S ++ + D KN R I LDQP +G S+ L G + + Y +YM ++ L+GA + E+ V F+ L IS D+ +L +KD L G W S+++ ++ +I
Sbjct: 37 IGCQQTAKTILENMDSTVNPCDDFYAFSCGGFINKTIVPNGEEKVNVLTKTKDGLIRDINDLMNEELNSSELQIFKDLKTFYKTCIDENKIEELGVTPMKDILKQMGGWPVIECDSWSKPRQTYRWYNETLKLRKLGFSAKYFLNFLVETDIKNPNKRIIMLDQPYVGFSKFLLQFG--NDGIIEYIQYMVNMAXLLGATEEKARKEMLQVFEFQKSLMNISIKDPNLTDQYELMK---VKDLRKLDG-CIDWQSYINGMISNSSI 296 HSP 4 Score: 90.8929 bits (224), Expect = 1.022e-18 Identity = 34/118 (28.81%), Postives = 74/118 (62.71%), Query Frame = 0
Query: 21 KMCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLE 138
K+C S C A + ++M+ +++PC++FY F+CG + T++P + + + L + L+ +E + + +IFKD++ FY++C++E++++++ P+ ++L +GGWPV+E
Sbjct: 32 KVCNSIGCQQTAKTILENMDSTVNPCDDFYAFSCGGFINKTIVPNGEEKVNVLTKTKDGLIRDINDLMNEELNSSELQIFKDLKTFYKTCIDENKIEELGVTPMKDILKQMGGWPVIE 149
BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Match: gi|1270743871|sp|A0A0B4K692.1|NEP2_DROME (RecName: Full=Neprilysin-2; Contains: RecName: Full=Neprilysin-2, soluble form) HSP 1 Score: 377.096 bits (967), Expect = 7.803e-113 Identity = 205/518 (39.58%), Postives = 294/518 (56.76%), Query Frame = 0
Query: 139 ENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP--SWTSFVDNLVYTH-NITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAA--GFGTFSESSFI---FAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
E L++G + V AY+KYM DI L GA+R+ + EL L FEM LA IS P E+RR+ ++LYN L+ L P W +++ L+ N+ EDE + + P FF+ L ++ K R +ANY+ WR VG + + R L+Y+ SG Q ARWK CV A E F + GSLY +F K++K+ E++ +R F +I++++ WMD TK +A KL +M IGYPDE+L+ +K+ + Y + ++ KYF SFL + + + +LR ++K W A+VNAFY +N I FPAG+L+G+FFN + P Y+N+GA+G DT K + KA+CII+Q NY + G+ LNGINTQGENIADNGG+ ++Y A+ + K+G + LPGL +TP Q+FW++ G+ WC K R E LK I H+P+EFRV G+LS K+FA+DF CP GSPMNP +KC
Sbjct: 257 EYLVKGFNETLVTAYYKYMVDIAVLFGANRDLAKTELLLSLEFEMALANISWPNEKRRNSSELYN---LRTPAQLQAAYPYVQWVDYMNALLPEGLNVAEDEMINLSVPSFFEDLGKLLAKTPKRVIANYMFWRIHGFSVGFLSEEFRKRQLQYATALSGRQEQEARWKECVDIATSSMDEVCEDDFDSLGISVGSLYVGKHFHKDSKANALEMVNEIRNVFNDILDEVNWMDAKTKKEAKLKLHSMATHIGYPDEMLDNEKLAAYYAKLDIDPDKYFESFLGMNIFGTDYSFNKLRLPVNKTDWVRHARPAIVNAFYSSLENSIQFPAGILQGHFFNAQRPKYMNFGAIGYVIGHEITHGFDDQGRQFDVKGNLRDWWHPDTQKAYLAKAKCIIEQYGNYTERATGLNLNGINTQGENIADNGGVKESYIAYRRWAEKHGPEAKLPGLDYTPEQMFWVAAGQTWCAKYRKESLKMRITTGVHSPSEFRVLGSLSNMKDFAKDFHCPEGSPMNPVQKC 771 HSP 2 Score: 375.555 bits (963), Expect = 2.387e-112 Identity = 204/479 (42.59%), Postives = 278/479 (58.04%), Query Frame = 0
Query: 885 EFFKKLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP--SWTSFVDNLVYTH-NITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSR-----NSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAYAK 1355
E KK G+SMD ++D I D +NST R I LDQ L LSREYL++G + V AY+KYM DI L GA+R+ ++ EL L FEM LA IS P E+RR+ ++LYN L+ L P W +++ L+ N+ EDE + + P FF+ L ++ K R +ANY+ WR VG + + R L+YA SG E ARW+ CV +S GSLY +F K++K ++ +R F +ILD+++WMD TK +A KL +M IG+PDE+L+ +K+ + Y + I ++F++ L + + + +LR V+K W A A+VNA YS +N I FPAGIL G FFN P Y N+GAIG VIGHEITHGFDDQGR+ D G+L DWW DT + + KA+CII Q N+ L LNGINTQGENIADNGG+ E+Y AY +
Sbjct: 213 EQVKKFRTAGFSMDYIIDFSIGVDLQNSTKRLIDLDQSSLALSREYLVKGFNETLVTAYYKYMVDIAVLFGANRDLAKTELLLSLEFEMALANISWPNEKRRNSSELYN---LRTPAQLQAAYPYVQWVDYMNALLPEGLNVAEDEMINLSVPSFFEDLGKLLAKTPKRVIANYMFWRIHGFSVGFLSEEFRKRQLQYATALSGRQEQEARWKECVDIATSSMDEVCEDDFDSLGISVGSLYVGKHFHKDSKANALEMVNEIRNVFNDILDEVNWMDAKTKKEAKLKLHSMATHIGYPDEMLDNEKLAAYYAKLDIDPDKYFESFLGMNIFGTDYSFNKLRLPVNKTDWVRHARPAIVNAFYSSLENSIQFPAGILQGHFFNAQRPKYMNFGAIGYVIGHEITHGFDDQGRQFDVKGNLRDWWHPDTQKAYLAKAKCIIEQYGNYTERATGLNLNGINTQGENIADNGGVKESYIAYRR 688 HSP 3 Score: 195.667 bits (496), Expect = 2.769e-50 Identity = 105/274 (38.32%), Postives = 163/274 (59.49%), Query Frame = 0
Query: 621 RSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTD-YKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP--SWTSFVDNLVYTH-NITEDERVLIGNPEFFKKL 890
R M ++PC+NFYEF CG +E IP+DK S S ++++ LQ++ K ++ E + + + F+ Y++C+N+ ++ + EP+T V + LGGWP+++G +W+ D + W E + G+SMD ++D I D +NST R I LDQ L LSREYL++G + V AY+KYM DI L GA+R+ ++ EL L FEM LA IS P E+RR+ ++LYN L+ L P W +++ L+ N+ EDE + + P FF+ L
Sbjct: 98 RKMKPEVEPCDNFYEFACGTYLEEENIPDDKVSISTFSVISDKLQEQLKDIITAERPETEPKHFRLPNLLYKACMNKTLIETLGPEPITRVAERLGGWPLIKGDSWNADDSWTWQEQVKKFRTAGFSMDYIIDFSIGVDLQNSTKRLIDLDQSSLALSREYLVKGFNETLVTAYYKYMVDIAVLFGANRDLAKTELLLSLEFEMALANISWPNEKRRNSSELYN---LRTPAQLQAAYPYVQWVDYMNALLPEGLNVAEDEMINLSVPSFFEDL 368 HSP 4 Score: 92.8189 bits (229), Expect = 9.800e-18 Identity = 38/117 (32.48%), Postives = 74/117 (63.25%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLE 138
+CL++ C+ A+ + + M ++PC+NFYEF CG +E IP+DK S S ++++ LQ++ K ++ E + + + F+ Y++C+N+ ++ + EP+T V + LGGWP+++
Sbjct: 83 VCLTQECIHTASTVLRKMKPEVEPCDNFYEFACGTYLEEENIPDDKVSISTFSVISDKLQEQLKDIITAERPETEPKHFRLPNLLYKACMNKTLIETLGPEPITRVAERLGGWPLIK 199
BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Match: gi|128062|sp|P08473.2|NEP_HUMAN (RecName: Full=Neprilysin; AltName: Full=Atriopeptidase; AltName: Full=Common acute lymphocytic leukemia antigen; Short=CALLA; AltName: Full=Enkephalinase; AltName: Full=Neutral endopeptidase 24.11; Short=NEP; Short=Neutral endopeptidase; AltName: Full=Skin fibroblast elastase; Short=SFE; AltName: CD_antigen=CD10) HSP 1 Score: 360.918 bits (925), Expect = 2.779e-107 Identity = 221/705 (31.35%), Postives = 351/705 (49.79%), Query Frame = 0
Query: 19 DFKMCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEE-----------------------------NLIEGLEDQ----HV--------------------------KAYFKYMQDITEL------MGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-----LPGHPPSWTSFVDNLVYTHNI--TEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEED-KVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVK-LNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCR 621
D +C S C+ A L Q+M+ + +PC +F+++ CG ++ VIPE S + IL +L+ K +L++ ++D + K + YRSC+NE +D EPL ++L + GWPV E NL G +D+ HV AY +M + L + D N E+ V+ E E+A +A E+R D LYN L ++ + G P SW +F + ++ T NI T +E V++ PE+ KL ++TK S R L N + WRF+ +V +R +++ + K GT + A W+ C + + + A G LY + F E+K ++ +LI +RE F + ++D+ WMD TK +A EK +A+ + IGYPD+I+ D K+ + Y + ++ +YF + + + S++ ++LRE++DK W + A+VNAFY +N I FPAG+L+ FF+ + +NYG +GM ++ F+E++QC++ Q N+ G + LNGINT GENIADNGGL QAY+A++ ++ KNG + +LPGL +QLF+++F +VWC R E+ I D H+P FR+ G L + EF+ F C S MNPE+KCR
Sbjct: 53 DDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQK-AKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCAN------YVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEW--ISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCR 748 HSP 2 Score: 314.694 bits (805), Expect = 9.017e-91 Identity = 185/477 (38.78%), Postives = 271/477 (56.81%), Query Frame = 0
Query: 895 YSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYL-IEGLEDQHVKAYFKYMQDITEL------MGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-----LPGHPPSWTSFVDNLVYTHNI--TEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEED-KVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNF--ESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
Y ++++L++ D KNS IH+DQP LGL SR+Y G+ + AY +M + L + D N E+ V+ E E+A +A E+R D LYN L ++ + G P SW +F + ++ T NI T +E V++ PE+ KL ++TK S R L N + WRF+ +V +R +++ + K GT A W C A + N+ G LY + F E+K ++ LI +R F + LDD+ WMD TK +A EK A+ + IG+PD+I+ D K+ + Y + +E ++F+NI+ + S+ Q ++LRE+VDK W A A+VNA YS +N I+FPAGIL FF+ NYG IG+VIGHEITHGFDD GR +++GDL DWW + +A F++++QC++ Q NF + A Q LNGINT GENIADNGG+ +AYRAY
Sbjct: 195 YGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRC--ANYVNGNMENA----VGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGA--AVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQ-HLNGINTLGENIADNGGLGQAYRAY 662 HSP 3 Score: 134.035 bits (336), Expect = 1.233e-30 Identity = 101/329 (30.70%), Postives = 165/329 (50.15%), Query Frame = 0
Query: 621 RSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDR---TDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYL-IEGLEDQHVKAYFKYMQDITEL------MGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-----LPGHPPSWTSFVDNLVYTHN--ITEDERVLIGNPEFFKKLSDEGYSMDMMVDLYIYGDAKN-STWRTIHLDQPVLGLSREY 930
++M+ + +PC +F+++ CG ++ VIPE S + IL +L+ K +L++ ++D + K + YRSC+NE +D EPL ++L + GWPV +NW++ + + I LN + Y ++++L + D KNS IH+DQP LGL SR+Y G+ + AY +M + L + D N E+ V+ E E+A +A E+R D LYN L ++ + G P SW +F + ++ T N IT +E V++ PE+ KL ++ Y D +N +WR I V LSR Y
Sbjct: 71 QNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQK-AKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVAT-ENWEQKYGASWTAEKAIAQLNSK-YGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKP-------ILTKYSARDLQNLMSWRFIM--DLVSSLSRTY 387
BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Match: gi|182702115|sp|Q5RE69.2|NEP_PONAB (RecName: Full=Neprilysin; AltName: Full=Atriopeptidase; AltName: Full=Enkephalinase; AltName: Full=Neutral endopeptidase 24.11; Short=NEP; Short=Neutral endopeptidase; AltName: Full=Skin fibroblast elastase; Short=SFE; AltName: CD_antigen=CD10) HSP 1 Score: 358.607 bits (919), Expect = 2.389e-106 Identity = 220/705 (31.21%), Postives = 350/705 (49.65%), Query Frame = 0
Query: 19 DFKMCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEE-----------------------------NLIEGLEDQ----HV--------------------------KAYFKYMQDITEL------MGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-----LPGHPPSWTSFVDNLVYTHNI--TEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEED-KVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVK-LNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCR 621
D +C S C+ A L Q+M+ + +PC +F+++ CG ++ VIPE S + IL +L+ K +L++ ++D + K + YRSC+NE +D EPL ++L + GWPV E NL G +D+ HV AY +M + L + D N E+ V+ E E+A +A E+R D LY L ++ + G P SW +F + ++ T NI T +E V++ PE+ KL ++TK S R L N + WRF+ +V +R +++ + K GT + A W+ C + + + A G LY + F E+K ++ +LI +RE F + ++D+ WMD TK +A EK +A+ + IGYPD+I+ D K+ + Y + ++ +YF + + + S++ ++LRE++DK W + A+VNAFY +N I FPAG+L+ FF+ + +NYG +GM ++ F+E++QC++ Q N+ G + LNGINT GENIADNGGL QAY+A++ ++ KNG + +LPGL +QLF+++F +VWC R E+ I D H+P FR+ G L + EF+ F C S MNPE+KCR
Sbjct: 53 DDGICKSSDCIKSAARLIQNMDATAEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQK-AKTLYRSCINESAIDSRGGEPLLKLLPDVYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYKKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCAN------YVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEW--ISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCR 748 HSP 2 Score: 312.383 bits (799), Expect = 5.913e-90 Identity = 184/477 (38.57%), Postives = 270/477 (56.60%), Query Frame = 0
Query: 895 YSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYL-IEGLEDQHVKAYFKYMQDITEL------MGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-----LPGHPPSWTSFVDNLVYTHNI--TEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEED-KVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNF--ESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
Y ++++L++ D KNS IH+DQP LGL SR+Y G+ + AY +M + L + D N E+ V+ E E+A +A E+R D LY L ++ + G P SW +F + ++ T NI T +E V++ PE+ KL ++TK S R L N + WRF+ +V +R +++ + K GT A W C A + N+ G LY + F E+K ++ LI +R F + LDD+ WMD TK +A EK A+ + IG+PD+I+ D K+ + Y + +E ++F+NI+ + S+ Q ++LRE+VDK W A A+VNA YS +N I+FPAGIL FF+ NYG IG+VIGHEITHGFDD GR +++GDL DWW + +A F++++QC++ Q NF + A Q LNGINT GENIADNGG+ +AYRAY
Sbjct: 195 YGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYKKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRC--ANYVNGNMENA----VGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGA--AVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQ-HLNGINTLGENIADNGGLGQAYRAY 662 HSP 3 Score: 131.339 bits (329), Expect = 9.021e-30 Identity = 100/329 (30.40%), Postives = 164/329 (49.85%), Query Frame = 0
Query: 621 RSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDR---TDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYL-IEGLEDQHVKAYFKYMQDITEL------MGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-----LPGHPPSWTSFVDNLVYTHN--ITEDERVLIGNPEFFKKLSDEGYSMDMMVDLYIYGDAKN-STWRTIHLDQPVLGLSREY 930
++M+ + +PC +F+++ CG ++ VIPE S + IL +L+ K +L++ ++D + K + YRSC+NE +D EPL ++L + GWPV +NW++ + + I LN + Y ++++L + D KNS IH+DQP LGL SR+Y G+ + AY +M + L + D N E+ V+ E E+A +A E+R D LY L ++ + G P SW +F + ++ T N IT +E V++ PE+ KL ++ Y D +N +WR I V LSR Y
Sbjct: 71 QNMDATAEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQK-AKTLYRSCINESAIDSRGGEPLLKLLPDVYGWPVAT-ENWEQKYGASWTAEKAIAQLNSK-YGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYKKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKP-------ILTKYSARDLQNLMSWRFIM--DLVSSLSRTY 387
BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Match: gi|2499916|sp|P97739.1|ECE1_CAVPO (RecName: Full=Endothelin-converting enzyme 1; Short=ECE-1) HSP 1 Score: 343.584 bits (880), Expect = 5.148e-101 Identity = 207/686 (30.17%), Postives = 347/686 (50.58%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGW----PVLEENLIEGLE-------------------------------------------------DQHVKAYFKYMQDITELMGA-DRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQ--ALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEIL---EEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
+CLSEACV + + + SMN ++DPC++F+ + CG ++ +P+ S W + L + Q K LLE + E + + +YR+C+NE ++++ +PL E+++ LGGW P ++N + L+ ++ + Y YM + +L+G D NA A+++ +L FE LA I+ P+E+RRD +Y+ + ++L +W F++ + Y I E E +++ + E+ +++S ++ L NY+ W V+ + +DA ++ +V GT+ LP RWK CVS +E++ F G ++ K F +++K+I +E+I ++++F E ++ ++WMD+ T+ A EK A+ MIGYP+ I+ E DKV + Y V YF + + S EQLR+ ++ W MVNA+Y +KN I FPAG+L F+ +P +N+G +G+ + + F+++ +C+++Q +NY G +NG +T GENIADNGGL AY+A++ + KNG + LP L T QLF++ F +VWC E ++ D H+P+ FRV G+LS +KEF+ FQCP GSPMNP KC
Sbjct: 82 VCLSEACVSVTSSILNSMNPTVDPCQDFFSYACGGWIKANPVPDGHSRWGAFSNLWEHNQAIIKHLLENSTASVS-EAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNRNVIHVDQSGLGLPSRDYYLNKTENEKVLNGYLNYMVQLGKLLGGGDENAIRAQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTL-APAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQVSTLINTTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLP-RWKFCVSD------TENNLGFGLGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAV---PDLYFENAMRFFNFSWRVTAEQLRKAPNRDQWSM--TPPMVNAYYSPTKNEIVFPAGILPAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVEQYSNYSVN--GEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEETLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFQCPPGSPMNPRHKC 751 HSP 2 Score: 284.648 bits (727), Expect = 2.668e-80 Identity = 159/464 (34.27%), Postives = 257/464 (55.39%), Query Frame = 0
Query: 895 YSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYLIEGLEDQHV-KAYFKYMQDITELMGA-DRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNE--LPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
Y +Y+ D+KNS IH+DQ LGL SR+Y + E++ V Y YM + +L+G D NA A+++ +L FE LA I+ P+E+RRD +Y+ + ++L +W F++ + Y I E E +++ + E+ +++S ++ L NY+ W V+ + +DA ++ +V GT + LP RW+ CV T N+ FG G ++ K F +++K +I ++ +F+E L + WMD+ T+ A EK A+ MIG+P+ I++ ++ ++ +F+N + S + E+LR+ ++ W T MVNA YSPTKN I+FPAGIL F+ +P N+G IGVV+GHE+TH FDDQGR D++G+L WW + + F+ + +C++ Q +N+ +NG +T GENIADNGG+ AYRAY
Sbjct: 215 YRTSPFFSVYVSADSKNSNRNVIHVDQSGLGLPSRDYYLNKTENEKVLNGYLNYMVQLGKLLGGGDENAIRAQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTL-APAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQVSTLINTTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLP-RWKFCVSDT------ENNLGFGLGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTAEQLRKAPNRDQWS--MTPPMVNAYYSPTKNEIVFPAGILPAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVEQYSNYSVNGE--PVNGRHTLGENIADNGGLKAAYRAY 666 HSP 3 Score: 139.813 bits (351), Expect = 2.003e-32 Identity = 79/273 (28.94%), Postives = 148/273 (54.21%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYLIEGLEDQHV-KAYFKYMQDITELMGA-DRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLS 891
SMN ++DPC++F+ + CG ++ +P+ S W + L + Q K LLE + E + + +YR+C+NE ++++ +PL E+++ LGGW + W + ++ ++ + Y +++ D+KNS IH+DQ LGL SR+Y + E++ V Y YM + +L+G D NA A+++ +L FE LA I+ P+E+RRD +Y+ + ++L +W F++ + Y I E E +++ + E+ +++S
Sbjct: 98 SMNPTVDPCQDFFSYACGGWIKANPVPDGHSRWGAFSNLWEHNQAIIKHLLENSTASVS-EAERKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNIT--GPWAKDNF---QDTLQVVTAHYRTSPFFSVYVSADSKNSNRNVIHVDQSGLGLPSRDYYLNKTENEKVLNGYLNYMVQLGKLLGGGDENAIRAQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTL-APAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQVS 363
BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Match: gi|1372196287|sp|B2RQR8.1|ECE2_MOUSE (RecName: Full=Endothelin-converting enzyme 2; Short=ECE-2) HSP 1 Score: 342.428 bits (877), Expect = 2.018e-100 Identity = 196/680 (28.82%), Postives = 334/680 (49.12%), Query Frame = 0
Query: 23 CLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGW----PVLEENLIEGLE-------------------------------------------------DQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQ-ALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
C++EAC+ +A + +S++R + PC++FY+F+CG + +P +S W+ L Q K LLE E + R FY SCL + ++K+ +PL +++D +GGW P E++ ++ L+ ++ + AY YM ++ L+G ++ +++ VL E++LA I+ P+++RRD K+Y+ + + ++L W F+ L+ + + E V++ E+ +++S+++ + P L NY+ W V+ + A + + GT+ + RW+TC+S ++ + FA GSL+ K F +++K I +I +R +F E + D+ WMD+ T+ A EK A+ MIG+PD ILE ++ Y+G V + +F + L+L S +QLR+ + W VNA+Y +KN I FPAG+L+ F+ H P +N+G +G+ ++ F+ C+ +Q + Y+ G +LNG+ T GENIADNGGL AY A++ L K+G + LP + T QLF++ F +VWC E ++ D H+P FRV G LS +++F R F CP+GSPMNP + C
Sbjct: 92 CVTEACIRVAGKILESLDRGVSPCQDFYQFSCGGWIRRNPLPNGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGWNITGPWDEDSFMDVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQAL-APAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLETLYGTKKSCTPRWQTCISN------TDDALGFALGSLFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVWMDEKTRLAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSM--TPQTVNAYYLPTKNEIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLTAFQNHTACMEEQYSQYQVN--GERLNGLQTLGENIADNGGLKAAYNAYKAWLRKHGEEQPLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLC 760 HSP 2 Score: 283.493 bits (724), Expect = 1.006e-79 Identity = 157/462 (33.98%), Postives = 255/462 (55.19%), Query Frame = 0
Query: 895 YSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYLIEGLEDQHV-KAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNE-LPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
Y +Y+ D+K+S I +DQ L L SR+Y + ++ V AY YM ++ L+G ++ +++ VL E++LA I+ P+++RRD K+Y+ + + ++L W F+ L+ + + E V++ E+ +++S+++ + P L NY+ W V+ + A + + GT + RW+TC+ T ++ F GSL+ K F +++K +G+I +R++F+E L D+ WMD+ T+ A EK A+ MIGFPD ILE ++ +Y G ++ E FQN+L+L S + ++LR+ + W T VNA Y PTKN I+FPAGIL F+ + P N+G IGVV+GHE+TH FDDQGR D+ G+L WW ++ F++ C+ Q + ++ +LNG+ T GENIADNGG+ AY AY
Sbjct: 225 YRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQAL-APAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLETLYGTKKSCTPRWQTCISNTD------DALGFALGSLFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVWMDEKTRLAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQW--SMTPQTVNAYYLPTKNEIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLTAFQNHTACMEEQYSQYQVNGE--RLNGLQTLGENIADNGGLKAAYNAY 675 HSP 3 Score: 127.487 bits (319), Expect = 1.660e-28 Identity = 73/273 (26.74%), Postives = 139/273 (50.92%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYLIEGLEDQHV-KAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSD 892
S++R + PC++FY+F+CG + +P +S W+ L Q K LLE E + R FY SCL + ++K+ +PL +++D +GGW + WD + ++ Y +++ D+K+S I +DQ L L SR+Y + ++ V AY YM ++ L+G ++ +++ VL E++LA I+ P+++RRD K+Y+ + + ++L W F+ L+ + + E V++ E+ +++S+
Sbjct: 107 SLDRGVSPCQDFYQFSCGGWIRRNPLPNGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGWNIT--GPWDEDSFM---DVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQAL-APAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVSE 373
BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Match: gi|74930356|sp|Q8IS64.1|ECE_LOCMI (RecName: Full=Endothelin-converting enzyme homolog; Short=ECE) HSP 1 Score: 340.502 bits (872), Expect = 3.376e-100 Identity = 211/692 (30.49%), Postives = 348/692 (50.29%), Query Frame = 0
Query: 23 CLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLN-EDEMDKIAYEPLTEVLDTLGG--------------------------------WPVLEEN-----------------------LIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP--SWTSFVDNLV--YTHNITEDERVLIGNPEFFKKLSDVMTKASPRT-----LANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVA-MVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
C+S +CV A + M+ + DPC +FY++ CG V IP+ KS W L + Q K +LE+ S+ E + + +Y SCL+ D ++ + +P+ ++L +GG W V E++ L E ++D+ + AY +YM I L+G + ++K V+ FE ELA+I +P+E+RRD KLYN + + + G PP +W +F N + T I++ E+V++ PE+ +KL+D++ + T L NY+ W+ V+S+ ++ RDA K G++ W+ CV T + + FA G+++ + F +K +I +R +F + +++WMD T+ A +K A+ MIG+PD IL +++ +Y+ + +++ +YF + L + + + E+L + ++K W G+ VNA+Y +KN I FPAG+L+ F++ P +NYGA+G+ T + F+++ QC++DQ +NY V NG T GENIADNGGL AY A+ + +N + LPGL F+ +QLF++SF +VWC S DE +K + D HAP +FRV G LS +EF+ +F+CP+GS MNP KC
Sbjct: 47 CVSMSCVTAAASVLSLMDATADPCSDFYQYACGGWVRANPIPDTKSMWGTFVKLEQQNQLVIKNVLEQPMSEFKSEAERKAKLYYMSCLDVNDTIETLGPKPMLDLLVKIGGWNITGNFSIKNWSLQKSLETLQNRYNMGGLFTWAVGEDDRDSKKHIIQIDQGGLTLPTRDNYLNETMDDKVLSAYLEYMTKIGVLLGGEEKNVRTQMKAVIEFETELAKIMSPQEDRRDEEKLYNNM---ELDKVQGRPPFINWHAFFSNAMENITRKISKKEKVVVYAPEYLEKLNDIIRNYTNTTDGKIILNNYLVWQTVRSMTSYLSKAFRDAYKGLRKALVGSEGGEKPWRYCV------TDTNNVIGFAIGAMFVREAFHGNSKPAAENMINQIRTAFKSNLKNLKWMDAETRRAAEKKADAISDMIGFPDYILNPEELDKKYKDLEIKEDEYFENNLRVNKYNLKSNLEKLDQPVNKTRW---GMTPPTVNAYYTPTKNQIVFPAGILQAPFYDIGHPKSMNYGAMGVVMGHELTHAFDDQGREYDQNGNLHKWWNNQTIEAFKKRTQCVVDQYSNYTVDNKHV--NGKQTLGENIADNGGLKAAYHAYLEWEQRNPRELPLPGLNFSHKQLFFLSFAQVWCSASTDEAIKLQLEKDPHAPPKFRVIGPLSNLQEFSTEFRCPLGSKMNPVHKC 724 HSP 2 Score: 303.138 bits (775), Expect = 5.366e-87 Identity = 174/472 (36.86%), Postives = 266/472 (56.36%), Query Frame = 0
Query: 895 YSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSRE--YLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP--SWTSFVDNLV--YTHNITEDERVLIGNPEFFKKLSDVMTKASPRT-----LANYIGWRFVKSIVGITTRDARDA--ALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
Y+M + + D ++S I +DQ L L YL E ++D+ + AY +YM I L+G + ++K V+ FE ELA+I +P+E+RRD KLYN + + + G PP +W +F N + T I++ E+V++ PE+ +KL+D++ + T L NY+ W+ V+S+ ++ RDA L A V S E P W CV T N F G+++ + F +K + +I +R +FK L ++ WMD T+ A +K A+ MIGFPD IL +++ YK ++I+E ++F+N L + + + + E+L + V+K W T VNA Y+PTKN I+FPAGIL F++ P NYGA+GVV+GHE+TH FDDQGR DQNG+L WW+ T + F+ + QC+++Q +N+ +NG T GENIADNGG+ AY AY
Sbjct: 183 YNMGGLFTWAVGEDDRDSKKHIIQIDQGGLTLPTRDNYLNETMDDKVLSAYLEYMTKIGVLLGGEEKNVRTQMKAVIEFETELAKIMSPQEDRRDEEKLYNNM---ELDKVQGRPPFINWHAFFSNAMENITRKISKKEKVVVYAPEYLEKLNDIIRNYTNTTDGKIILNNYLVWQTVRSMTSYLSKAFRDAYKGLRKALVGSEGGEKP--WRYCVTDTN------NVIGFAIGAMFVREAFHGNSKPAAENMINQIRTAFKSNLKNLKWMDAETRRAAEKKADAISDMIGFPDYILNPEELDKKYKDLEIKEDEYFENNLRVNKYNLKSNLEKLDQPVNKTRWG--MTPPTVNAYYTPTKNQIVFPAGILQAPFYDIGHPKSMNYGAMGVVMGHELTHAFDDQGREYDQNGNLHKWWNNQTIEAFKKRTQCVVDQYSNYTVDNKH--VNGKQTLGENIADNGGLKAAYHAY 639 HSP 3 Score: 134.806 bits (338), Expect = 7.470e-31 Identity = 88/280 (31.43%), Postives = 147/280 (52.50%), Query Frame = 0
Query: 623 MNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLN-EDEMDKIAYEPLTEVLDTLGGWPV---LEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSRE--YLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP--SWTSFVDNLV--YTHNITEDERVLIGNPEFFKKLSD 892
M+ + DPC +FY++ CG V IP+ KS W L + Q K +LE+ S+ E + + +Y SCL+ D ++ + +P+ ++L +GGW + KNW K E + + Y+M + + D ++S I +DQ L L YL E ++D+ + AY +YM I L+G + ++K V+ FE ELA+I +P+E+RRD KLYN + + + G PP +W +F N + T I++ E+V++ PE+ +KL+D
Sbjct: 63 MDATADPCSDFYQYACGGWVRANPIPDTKSMWGTFVKLEQQNQLVIKNVLEQPMSEFKSEAERKAKLYYMSCLDVNDTIETLGPKPMLDLLVKIGGWNITGNFSIKNWSLQ--KSLETL----QNRYNMGGLFTWAVGEDDRDSKKHIIQIDQGGLTLPTRDNYLNETMDDKVLSAYLEYMTKIGVLLGGEEKNVRTQMKAVIEFETELAKIMSPQEDRRDEEKLYNNM---ELDKVQGRPPFINWHAFFSNAMENITRKISKKEKVVVYAPEYLEKLND 333
BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Match: gi|1706564|sp|P42893.2|ECE1_RAT (RecName: Full=Endothelin-converting enzyme 1; Short=ECE-1) HSP 1 Score: 341.658 bits (875), Expect = 3.397e-100 Identity = 203/686 (29.59%), Postives = 349/686 (50.87%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGW----PVLEENLIEGLE-------------------------------------------------DQHVKAYFKYMQDITELMGA-DRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQ--ALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEIL---EEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
+CL+EACV + + + SM+ ++DPC++F+ + CG ++ +P+ S W + L + Q K LLE + E K + +YR+C+NE ++++ +PL E+++ LGGW P ++N + L+ ++ + Y YM + +L+G D ++ +++ +L FE LA I+ P+E+RRD +Y+ + ++L +W F++ + Y I E E +++ + E+ +++S ++ L NY+ W V+ + +DA ++ +V GT+ LP RWK CVS +E++ FA G ++ K F +++K+I +E+I ++++F E ++ ++WMD+ T+ A EK A+ MIGYP+ I+ E DKV + Y V YF + + S +QLR+ ++ W MVNA+Y +KN I FPAG+L+ F+ +P +N+G +G+ + + F+++ +C++ Q NNY G +NG +T GENIADNGGL AY+A++ + KNG + +LP L T QLF++ F +VWC E ++ D H+P+ FRV G+LS +KEF+ F+CP+GSPMNP KC
Sbjct: 90 VCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASAS-EAEKKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDSIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTL-APAINWLPFLNAIFYPVEINESEPIVVYDKEYLRQVSTLINSTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLP-RWKFCVSD------TENNLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAV---PDLYFENAMRFFNFSLRVTADQLRKAPNRDQWSM--TPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYNNYSVN--GEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQILPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPLGSPMNPRHKC 759 HSP 2 Score: 275.404 bits (703), Expect = 5.389e-77 Identity = 154/464 (33.19%), Postives = 254/464 (54.74%), Query Frame = 0
Query: 895 YSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYLIEGLEDQHV-KAYFKYMQDITELMGA-DRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNE--LPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
Y +Y+ D+KNS I +DQ LGL SR+Y + E++ V Y YM + +L+G D ++ +++ +L FE LA I+ P+E+RRD +Y+ + ++L +W F++ + Y I E E +++ + E+ +++S ++ L NY+ W V+ + +DA ++ +V GT + LP RW+ CV T N+ F G ++ K F +++K +I ++ +F+E L + WMD+ T+ A EK A+ MIG+P+ I++ ++ ++ +F+N + S + ++LR+ ++ W T MVNA YSPTKN I+FPAGIL F+ +P N+G IGVV+GHE+TH FDDQGR D++G+L WW + + F+ + +C++ Q NN+ +NG +T GENIADNGG+ AYRAY
Sbjct: 223 YRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDSIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTL-APAINWLPFLNAIFYPVEINESEPIVVYDKEYLRQVSTLINSTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLP-RWKFCVSDT------ENNLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSLRVTADQLRKAPNRDQWS--MTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYNNYSVNGE--PVNGRHTLGENIADNGGLKAAYRAY 674 HSP 3 Score: 131.724 bits (330), Expect = 6.916e-30 Identity = 75/273 (27.47%), Postives = 146/273 (53.48%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYLIEGLEDQHV-KAYFKYMQDITELM-GADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLS 891
SM+ ++DPC++F+ + CG ++ +P+ S W + L + Q K LLE + E K + +YR+C+NE ++++ +PL E+++ LGGW + W + + +++ + Y +++ D+KNS I +DQ LGL SR+Y + E++ V Y YM + +L+ G D ++ +++ +L FE LA I+ P+E+RRD +Y+ + ++L +W F++ + Y I E E +++ + E+ +++S
Sbjct: 106 SMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASAS-EAEKKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNIT--GPWAKDN---FQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDSIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTL-APAINWLPFLNAIFYPVEINESEPIVVYDKEYLRQVS 371
BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Match: gi|114150029|sp|P0C1T0.1|MMEL1_RAT (RecName: Full=Membrane metallo-endopeptidase-like 1; AltName: Full=NEP2(m); AltName: Full=Neprilysin II; Short=NEPII; AltName: Full=Neprilysin-2; Short=NEP2; Short=NL2; Contains: RecName: Full=Membrane metallo-endopeptidase-like 1, soluble form; AltName: Full=Neprilysin-2 secreted; Short=NEP2(s)) HSP 1 Score: 339.347 bits (869), Expect = 3.167e-99 Identity = 217/698 (31.09%), Postives = 347/698 (49.71%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEENLIEGL-----------------------------EDQ----HV-------------KAYFK------------YMQDITELMGADRNAS------HAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP-SWTSFVDNLVYTHNI--TEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDK--VTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMAD----------------------------TSKKFEEKAQCIIDQANNYESKQV-GVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCR 621
+C + +CV A + Q+M++S PC+NFY++ CG + + VIPE S +S IL +L+ K +LE Q + K + YRSC+N+ ++K EPL VLD +GGWPV + E + +DQ HV + YFK +M + ++ D N E+ VL E LA + P+E+R D LY+ L++ + G +WT F+ N++ + + +E V++ + + L +++ +TL NY+ WR V +G ++ ++A ++Y K GT RW+ CVS + S+ A GSLY K F K++KSI++ELIE +R F + ++++ WMD+ +K KA EK + + + IGYPD ILE++ + Y + + YF + L + + ++LRE++D+ W + A+VNAFY ++N+I FPAG+L+ FF+ P +N+G +GM +++ F +++QC+I Q +N+ + +NG +T GENIADNGG+ QAYKA+ + LA+ G D LPGL T QLF+I++ +VWC R E I D H+P +V G+L F+ F CP GSPM+P +CR
Sbjct: 84 ICTTPSCVIAAARILQNMDQSKKPCDNFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGVLEDSSVQHRPAVEK-AKTLYRSCMNQSVIEKRDSEPLLNVLDMIGGWPVAMDKWNETMGPKWELERQLAVLNSQFNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFKEDSHRVREAYLQFMTSVATMLRRDLNLPGETDLVQEEMAQVLHLETHLANATVPQEKRHDVTALYHRMGLEELQERFGLKGFNWTLFIQNVLSSVQVELLPNEEVVVYGIPYLENLEEIIDVFPAQTLQNYLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTMEEVRWRECVS------YVNSNMESAVGSLYIKRAFSKDSKSIVSELIEKIRSVFVDNLDELNWMDEESKKKAQEKALNIREQIGYPDYILEDNNRHLDEEYSSLTFSEDLYFENGLQNLKNNAQRSLKKLREKVDQNLW--IIGAAVVNAFYSPNRNLIVFPAGILQPPFFSKDQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMLDWWSNFSARHFRQQSQCMIYQYSNFSWELADNQNVNGFSTLGENIADNGGVRQAYKAYLQWLAEGGRDQRLPGLNLTYAQLFFINYAQVWCGSYRPEFAIQSIKTDVHSPLNAQVLGSLQNLPGFSEAFHCPRGSPMHPMNRCR 772 HSP 2 Score: 307.375 bits (786), Expect = 4.964e-88 Identity = 178/482 (36.93%), Postives = 277/482 (57.47%), Query Frame = 0
Query: 895 YSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYLIEGLEDQHV--KAYFKYMQDITELMGADRNAS------EAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP-SWTSFVDNLVYTHNI--TEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDK--VTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNF--ESAQVQLKLNGINTQGENIADNGGISEAYRAYAKNLASG 1360
++ +++DL+I+ D +NS+ I++DQP LG+ SREY + ED H +AY ++M + ++ D N + E+ VL E LA + P+E+R D LY+ L++ + G +WT F+ N++ + + +E V++ + + L +++ +TL NY+ WR V +G ++ ++A ++Y K GT RW CV S GSLY K F K++K + LI +R+ F + LD+++WMD+ +K KA EK + + IG+PD ILE++ + Y + E +F+N L + + Q ++LRE+VD+ W A A+VNA YSP +N+I+FPAGIL FF+ D P N+G IG+VIGHEITHGFDD GR D+NG++ DWW +A+ F ++QC+I Q +NF E A Q +NG +T GENIADNGG+ +AY+AY + LA G
Sbjct: 223 FNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFK--EDSHRVREAYLQFMTSVATMLRRDLNLPGETDLVQEEMAQVLHLETHLANATVPQEKRHDVTALYHRMGLEELQERFGLKGFNWTLFIQNVLSSVQVELLPNEEVVVYGIPYLENLEEIIDVFPAQTLQNYLVWRLVLDRIGSLSQRFKEARVDYRKALYGTTMEEVRWRECVSYVNSNMES------AVGSLYIKRAFSKDSKSIVSELIEKIRSVFVDNLDELNWMDEESKKKAQEKALNIREQIGYPDYILEDNNRHLDEEYSSLTFSEDLYFENGLQNLKNNAQRSLKKLREKVDQNLWIIGA--AVVNAFYSPNRNLIVFPAGILQPPFFSKDQPQALNFGGIGMVIGHEITHGFDDNGRNFDKNGNMLDWWSNFSARHFRQQSQCMIYQYSNFSWELADNQ-NVNGFSTLGENIADNGGVRQAYKAYLQWLAEG 693 HSP 3 Score: 135.961 bits (341), Expect = 3.262e-31 Identity = 92/280 (32.86%), Postives = 150/280 (53.57%), Query Frame = 0
Query: 621 RSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRT---DYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYLIEGLEDQH--VKAYFKYMQDITELMGADRNAS------EAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPP-SWTSFVDNL---VYTHNITEDERVLIGNP 884
++M++S PC+NFY++ CG + + VIPE S +S IL +L+ K +LE Q + K + YRSC+N+ ++K EPL VLD +GGWPV K W+ T ++ + LN + ++ +++DL I+ D +NS+ I++DQP LG+ SREY + ED H +AY ++M + ++ D N + E+ VL E LA + P+E+R D LY+ L++ + G +WT F+ N+ V + +E V+ G P
Sbjct: 99 QNMDQSKKPCDNFYQYACGGWLRHHVIPETNSRYSVFDILRDELEVILKGVLEDSSVQHRPAVEK-AKTLYRSCMNQSVIEKRDSEPLLNVLDMIGGWPVAMDK-WNETMGPKWELERQLAVLNSQ-FNRRVLIDLFIWNDDQNSSRHVIYIDQPTLGMPSREYYFK--EDSHRVREAYLQFMTSVATMLRRDLNLPGETDLVQEEMAQVLHLETHLANATVPQEKRHDVTALYHRMGLEELQERFGLKGFNWTLFIQNVLSSVQVELLPNEEVVVYGIP 373
BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Match: gi|1352911976|sp|P0DPD9.1|EFCE2_MOUSE (RecName: Full=EEF1AKMT4-ECE2 readthrough transcript protein; Includes: RecName: Full=Methyltransferase-like region; Includes: RecName: Full=Endothelin-converting enzyme 2 region) HSP 1 Score: 342.043 bits (876), Expect = 3.292e-99 Identity = 196/680 (28.82%), Postives = 334/680 (49.12%), Query Frame = 0
Query: 23 CLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGW----PVLEENLIEGLE-------------------------------------------------DQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQ-ALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
C++EAC+ +A + +S++R + PC++FY+F+CG + +P +S W+ L Q K LLE E + R FY SCL + ++K+ +PL +++D +GGW P E++ ++ L+ ++ + AY YM ++ L+G ++ +++ VL E++LA I+ P+++RRD K+Y+ + + ++L W F+ L+ + + E V++ E+ +++S+++ + P L NY+ W V+ + A + + GT+ + RW+TC+S ++ + FA GSL+ K F +++K I +I +R +F E + D+ WMD+ T+ A EK A+ MIG+PD ILE ++ Y+G V + +F + L+L S +QLR+ + W VNA+Y +KN I FPAG+L+ F+ H P +N+G +G+ ++ F+ C+ +Q + Y+ G +LNG+ T GENIADNGGL AY A++ L K+G + LP + T QLF++ F +VWC E ++ D H+P FRV G LS +++F R F CP+GSPMNP + C
Sbjct: 210 CVTEACIRVAGKILESLDRGVSPCQDFYQFSCGGWIRRNPLPNGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGWNITGPWDEDSFMDVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQAL-APAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLETLYGTKKSCTPRWQTCISN------TDDALGFALGSLFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVWMDEKTRLAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSM--TPQTVNAYYLPTKNEIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLTAFQNHTACMEEQYSQYQVN--GERLNGLQTLGENIADNGGLKAAYNAYKAWLRKHGEEQPLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLC 878 HSP 2 Score: 283.493 bits (724), Expect = 6.866e-79 Identity = 157/462 (33.98%), Postives = 255/462 (55.19%), Query Frame = 0
Query: 895 YSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYLIEGLEDQHV-KAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNE-LPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
Y +Y+ D+K+S I +DQ L L SR+Y + ++ V AY YM ++ L+G ++ +++ VL E++LA I+ P+++RRD K+Y+ + + ++L W F+ L+ + + E V++ E+ +++S+++ + P L NY+ W V+ + A + + GT + RW+TC+ T ++ F GSL+ K F +++K +G+I +R++F+E L D+ WMD+ T+ A EK A+ MIGFPD ILE ++ +Y G ++ E FQN+L+L S + ++LR+ + W T VNA Y PTKN I+FPAGIL F+ + P N+G IGVV+GHE+TH FDDQGR D+ G+L WW ++ F++ C+ Q + ++ +LNG+ T GENIADNGG+ AY AY
Sbjct: 343 YRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQAL-APAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLETLYGTKKSCTPRWQTCISNTD------DALGFALGSLFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVWMDEKTRLAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWS--MTPQTVNAYYLPTKNEIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLTAFQNHTACMEEQYSQYQVNGE--RLNGLQTLGENIADNGGLKAAYNAY 793 HSP 3 Score: 127.487 bits (319), Expect = 2.349e-28 Identity = 73/273 (26.74%), Postives = 139/273 (50.92%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYLIEGLEDQHV-KAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPSWTSFVDNLVYTHNITEDERVLIGNPEFFKKLSD 892
S++R + PC++FY+F+CG + +P +S W+ L Q K LLE E + R FY SCL + ++K+ +PL +++D +GGW + WD + ++ Y +++ D+K+S I +DQ L L SR+Y + ++ V AY YM ++ L+G ++ +++ VL E++LA I+ P+++RRD K+Y+ + + ++L W F+ L+ + + E V++ E+ +++S+
Sbjct: 225 SLDRGVSPCQDFYQFSCGGWIRRNPLPNGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGWNIT--GPWDEDSFM---DVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQAL-APAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVSE 491
BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Match: gi|1352911972|sp|P0DPD6.1|ECE2_HUMAN (RecName: Full=Endothelin-converting enzyme 2; Short=ECE-2) HSP 1 Score: 339.347 bits (869), Expect = 7.555e-99 Identity = 198/682 (29.03%), Postives = 336/682 (49.27%), Query Frame = 0
Query: 23 CLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGW----PVLEENLIEGLE------------------------------DQH-------------------VKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPS--WTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQ-ALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
CL+EAC+ +A + +S++R + PCE+FY+F+CG + +P+ +S W+ L Q K LLE E + ++FY SCL + ++++ +PL ++++ +GGW P ++N +E L+ DQ + AY YM+++ L+G ++ +++ VL E++LA I+ P+++RRD K+Y+ + + ++L PS W F+ L+ +++ E V++ ++ +++S+++ + P L NY+ W V+ R A + + GT+ + RW+TC+S ++ + FA GSL+ K F +++K I +I +R +F E + + WMD+ T+ A EK A+ MIG+PD ILE ++ Y+G + + +F + L+L S +QLR+ + W VNA+Y +KN I FPAG+L+ F+ P +N+G +G+ ++ F C+ +Q N Y+ G +LNG T GENIADNGGL AY A++ L K+G + LP + T QLF++ F +VWC E ++ D H+P FRV G LS +++F R F CP+GSPMNP + C
Sbjct: 140 CLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALA---PSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISN------TDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSM--TPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVN--GERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLC 808 HSP 2 Score: 284.263 bits (726), Expect = 1.060e-79 Identity = 161/464 (34.70%), Postives = 255/464 (54.96%), Query Frame = 0
Query: 895 YSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYLIEGLEDQHV-KAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPS--WTSFVDNLVYTHNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNE-LPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
Y +YI D+K+S I +DQ L L SR+Y + ++ V AY YM+++ L+G ++ +++ VL E++LA I+ P+++RRD K+Y+ + + ++L PS W F+ L+ +++ E V++ ++ +++S+++ + P L NY+ W V+ R A + + GT + RW+TC+ T ++ F GSL+ K F +++K +G+I +R +F+E L + WMD+ T+ A EK A+ MIGFPD ILE ++ +Y G +I E FQN+L+L S + ++LR+ + W T VNA Y PTKN I+FPAGIL F+ + P N+G IGVV+GHE+TH FDDQGR D+ G+L WW ++ F + C+ Q N ++ +LNG T GENIADNGG+ AY AY
Sbjct: 273 YRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALA---PSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTD------DALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWS--MTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGE--RLNGRQTLGENIADNGGLKAAYNAY 723 HSP 3 Score: 126.716 bits (317), Expect = 3.017e-28 Identity = 73/275 (26.55%), Postives = 146/275 (53.09%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYLIEGLEDQHV-KAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESLPGHPPS--WTSFVDNLVYTHNITEDERVLIGNPEFFKKLSD 892
S++R + PCE+FY+F+CG + +P+ +S W+ L Q K LLE E + ++FY SCL + ++++ +PL ++++ +GGW + WD+ ++ + Y ++I D+K+S I +DQ L L SR+Y + ++ V AY YM+++ L+G ++ +++ VL E++LA I+ P+++RRD K+Y+ + + ++L PS W F+ L+ +++ E V++ ++ +++S+
Sbjct: 155 SLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNIT--GPWDQDNFM---EVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQAL---APSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSE 421
BLAST of PREDICTED: neprilysin-2-like vs. nr
Match: gi|546677064|gb|ERL87973.1| (hypothetical protein D910_05361 [Dendroctonus ponderosae]) HSP 1 Score: 471.47 bits (1212), Expect = 8.423e-146 Identity = 245/691 (35.46%), Postives = 378/691 (54.70%), Query Frame = 0
Query: 7 TMRIGNPGPGEEDFKMCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLE----------------------------------------------ENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCR 621
T I P +E KMCL+ C+ A+ + + M++S+DPC++FY+FTCG ++NT IP+DKSS + +I++ LQ++ + ++E+ D+ + F+ +K Y++C+N+ ++ + ++L LGGWP LE E L +GL D+ VKAY+ YM DI L GADR + +L + L FEM+LA IS P E+RR+ LYNP +++ + P P W +++ L+ ++ +E +++ P++ ++++ R ANY+ WR V S V + D R L+Y+ + +G ARWK C+ S S AAG+LY + +F +EA+ E++ ++R F +I+ ++WMDD T+ A++K +M I YPDE+L + K+ YEG+ ++ +Y S L+L F +++LR+ ++K W G A+VNAFY +N I FPAG+L+G FF+ P Y+NYGA+G +T K F +KAQCIIDQ NY ++ + LNGINTQGENIAD GG+ ++Y A+ K +NG +P LPGL F RQ+FWIS WC +R E L+ L++ D HAP+ +RV G LS + FA DF CP GS MNP +KC+
Sbjct: 49 TYVINAPDDAKEGQKMCLTPGCIHTASRVLEYMDQSVDPCDDFYQFTCGNFIKNTNIPDDKSSVTSFSIISDTLQEQLRTMIEEPLKADEPKPFQLTKKLYKACMNKTIIEADGLNTMRQILKQLGGWPALEGSRWNEGDFDWRQSVYKFRTAGYSVDYFIDFSVGIDLDQGSLGLRREFLTKGLNDKLVKAYYDYMVDIAVLFGADRAVASKDLAESLDFEMKLANISLPSEKRRNATALYNPMSIRELQQKFPSIP--WLEYINTLLAPDTRVSNEEVIVVSIPKYISDFEALVSRTPKRAQANYVMWRAVASSVSYLSDDLRKRQLDYTTIVTGRTEREARWKECID------ISAGSLSIAAGALYVRKFFNEEARQNAKEMVSDIRAEFQDILKKVDWMDDKTRQNALDKARSMSTHIAYPDEMLNDAKLEEFYEGLELDQNQYLRSILNLTLFGTRFSFKRLRQPVNKTDWITHGRPAVVNAFYSAIENSIQFPAGILQGVFFSADRPRYMNYGAIGFVIGHEITHGFDDQGRQFDKEGNLVDWWQEETKKAFVQKAQCIIDQYGNYTVPELKLNLNGINTQGENIADIGGIKESYLAYRKWTGRNGNEPFLPGLKFNGRQMFWISAALTWCSATRVEELRQLVITDEHAPDRYRVIGPLSDMEYFAEDFGCPKGSKMNPVQKCQ 731 HSP 2 Score: 389.423 bits (999), Expect = 2.196e-115 Identity = 196/469 (41.79%), Postives = 283/469 (60.34%), Query Frame = 0
Query: 889 KLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAYAK 1355
K GYS+D +D + I LDQ LGL RE+L +GL D+ VKAY+ YM DI L GADR + +L + L FEM+LA IS P E+RR+ LYNP +++ + P P W +++ L+ ++ +E +++ P++ ++++ R ANY+ WR V S V + D R L+Y + +G E ARW+ C+ S S G+LY + +F +EA+ K ++ ++RA F++IL +DWMDD T+ A +K +M I +PDE+L + K+ Y+G+++ + Q+ ++IL+L +F ++RLR+ V+K W A+VNA YS +N I FPAGIL G FF+ D P Y NYGAIG VIGHEITHGFDDQGR+ D+ G+L DWW E+T + F KAQCII+Q N+ +++L LNGINTQGENIAD GGI E+Y AY K
Sbjct: 197 KFRTAGYSVDYFIDFSV----------GIDLDQGSLGLRREFLTKGLNDKLVKAYYDYMVDIAVLFGADRAVASKDLAESLDFEMKLANISLPSEKRRNATALYNPMSIRELQQKFPSIP--WLEYINTLLAPDTRVSNEEVIVVSIPKYISDFEALVSRTPKRAQANYVMWRAVASSVSYLSDDLRKRQLDYTTIVTGRTEREARWKECIDI------SAGSLSIAAGALYVRKFFNEEARQNAKEMVSDIRAEFQDILKKVDWMDDKTRQNALDKARSMSTHIAYPDEMLNDAKLEEFYEGLELDQNQYLRSILNLTLFGTRFSFKRLRQPVNKTDWITHGRPAVVNAFYSAIENSIQFPAGILQGVFFSADRPRYMNYGAIGFVIGHEITHGFDDQGRQFDKEGNLVDWWQEETKKAFVQKAQCIIDQYGNYTVPELKLNLNGINTQGENIADIGGIKESYLAYRK 647 HSP 3 Score: 200.675 bits (509), Expect = 9.894e-50 Identity = 96/260 (36.92%), Postives = 157/260 (60.38%), Query Frame = 0
Query: 623 MNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHNITEDERVLI 881
M++S+DPC++FY+FTCG ++NT IP+DKSS + +I++ LQ++ + ++E+ D+ + F+ +K Y++C+N+ ++ + ++L LGGWP LEG W+ D+ W +++Y GYS+D +D + I LDQ LGL RE+L +GL D+ VKAY+ YM DI L GADR + +L + L FEM+LA IS P E+RR+ LYNP +++ + P P W +++ L+ +E V++
Sbjct: 81 MDQSVDPCDDFYQFTCGNFIKNTNIPDDKSSVTSFSIISDTLQEQLRTMIEEPLKADEPKPFQLTKKLYKACMNKTIIEADGLNTMRQILKQLGGWPALEGSRWNEGDFDWRQSVYKFRTAGYSVDYFIDFSV----------GIDLDQGSLGLRREFLTKGLNDKLVKAYYDYMVDIAVLFGADRAVASKDLAESLDFEMKLANISLPSEKRRNATALYNPMSIRELQQKFPSIP--WLEYINTLLAPDTRVSNEEVIV 328
BLAST of PREDICTED: neprilysin-2-like vs. nr
Match: gi|1325280747|ref|XP_023325038.1| (neprilysin-2-like [Eurytemora affinis]) HSP 1 Score: 457.988 bits (1177), Expect = 9.475e-141 Identity = 240/692 (34.68%), Postives = 374/692 (54.05%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEM----------------------------DKIAYEPLTEVLDTLG----------------------------GWPVLEENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESL-PGHPPSWTSFVDNLVYTHN-----ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFG-TFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVG----------------------------MADTSKKFEEKAQCIIDQANNYESKQVG--VKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
+C++ CV A L + +N MDPCE+FYEF CG ++ T IP+DK+S +L + L + + L E D+ E++K +RK++ +C++ED + D ++ L EV + +G G + E LI+G ED+ V+AY+ YM D +GA R + ELK+ L+FE+++A+I+ P+EERR+ + L N +++ L PG +WT ++N+ HN +T +E + + P++ KL++ + R +NY WR++KSI+ + ++ R L++SK +G + L RW+TCV A F E S A GS+YAK YFP+ +K + E++ ++++ F +++++EWMD +TK A K+ M Q+IGY EI+ + YEG+ + Y ++L L + + R+ ID ++W G A+VNAFY +N I FPAG+L G FF P+Y+NYGA+G +T + + ++AQCII+Q N+ + +NGINTQGENIADNGG QAY+A+E+ + G + LPGLP+TPRQLFW+S +WC RDE LK+ +L D H+P+ FRVNG+ KEF++D+ CP SPMNPE+KC
Sbjct: 38 VCITPGCVKSAATLLEMINPEMDPCEDFYEFACGTFIKETTIPDDKTSIGSFTVLGEKLNMRLRKLFESSPKPDEPEVYKSVRKYFSACMDEDNIEETGRQELLRIINELGGWPTLQEDQWNQDNFSFHDLEEVANRIGLFTGVILSFGISTNLDNSTRRIMEVDQPGLGLDREYLIKGFEDKDVQAYYSYMVDTAVYLGAQRKVAEQELKESLMFELKIAEITLPQEERRNTSALNNQMTIEEGNKLYPGF--NWTQHINNM---HNSLEVPLTSNEVINVAVPKYIIKLAEYLPTVPARVQSNYQVWRYIKSILSLMDKEVRQIQLKFSKALTGKEELSPRWETCVKATSSKFYFYEGSLTNAIGSMYAKEYFPESSKLVAEEMVNSIKDEFKLMLDELEWMDAITKSAAQTKVDKMAQIIGYAREIINNTLINEFYEGLSISSTSYLQNYLDLSNYISNYYIREFRKPIDNQSWKNRGGAAVVNAFYSSKENSIKFPAGILDGIFFKDDRPSYMNYGAIGSVVGHEITHGFDDRGSQKDGDGNLVNWWQQETKQTYLQRAQCIIEQYGNFTVDVADEVLNVNGINTQGENIADNGGFKQAYRAYERLVKMKGEELSLPGLPYTPRQLFWLSGASIWCSSIRDEALKSRVLTDPHSPSRFRVNGSFRNQKEFSKDWNCPRESPMNPEKKC 724 HSP 2 Score: 386.726 bits (992), Expect = 2.386e-114 Identity = 195/471 (41.40%), Postives = 287/471 (60.93%), Query Frame = 0
Query: 894 GYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESL-PGHPPSWTSFVDNLVYTHN-----ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAH-SRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNF--ESAQVQLKLNGINTQGENIADNGGISEAYRAYAK 1355
G +++ I + NST R + +DQP LGL REYLI+G ED+ V+AY+ YM D +GA R +E ELK+ L+FE+++A+I+ P+EERR+ + L N +++ L PG +WT ++N+ HN +T +E + + P++ KL++ + R +NY WR++KSI+ + ++ R L+++K +G EL RWETCVKAT + S GS+YAK YFP+ +KL + ++ +++ FK +LD+++WMD TK A K+ M Q+IG+ EI+ + Y+G+ I + QN L L + R+ +D ++W R A+VNA YS +N I FPAGIL G FF D P Y NYGAIG V+GHEITHGFDD+G + D +G+L +WW ++T Q + +AQCII Q NF + A L +NGINTQGENIADNGG +AYRAY +
Sbjct: 176 GLFTGVILSFGISTNLDNSTRRIMEVDQPGLGLDREYLIKGFEDKDVQAYYSYMVDTAVYLGAQRKVAEQELKESLMFELKIAEITLPQEERRNTSALNNQMTIEEGNKLYPGF--NWTQHINNM---HNSLEVPLTSNEVINVAVPKYIIKLAEYLPTVPARVQSNYQVWRYIKSILSLMDKEVRQIQLKFSKALTGKEELSPRWETCVKATSSKFYFYEGSLTNAIGSMYAKEYFPESSKLVAEEMVNSIKDEFKLMLDELEWMDAITKSAAQTKVDKMAQIIGYAREIINNTLINEFYEGLSISSTSYLQNYLDLSNYISNYYIREFRKPIDNQSWKNRGGAAVVNAFYSSKENSIKFPAGILDGIFFKDDRPSYMNYGAIGSVVGHEITHGFDDRGSQKDGDGNLVNWWQQETKQTYLQRAQCIIEQYGNFTVDVADEVLNVNGINTQGENIADNGGFKQAYRAYER 641 HSP 3 Score: 199.904 bits (507), Expect = 1.765e-49 Identity = 97/276 (35.14%), Postives = 169/276 (61.23%), Query Frame = 0
Query: 623 MNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFESL-PGHPPSWTSFVDNLVYTHN-----ITEDERVLIGNPEFFKKLSD 892
+N MDPCE+FYEF CG ++ T IP+DK+S +L + L + + L E D+ E++K +RK++ +C++ED +++ + L +++ LGGWP L+ W++ ++ +++ N+ G +++ I + NST R + +DQP LGL REYLI+G ED+ V+AY+ YM D +GA R +E ELK+ L+FE+++A+I+ P+EERR+ + L N +++ L PG +WT ++N+ HN +T +E + + P++ KL++
Sbjct: 55 INPEMDPCEDFYEFACGTFIKETTIPDDKTSIGSFTVLGEKLNMRLRKLFESSPKPDEPEVYKSVRKYFSACMDEDNIEETGRQELLRIINELGGWPTLQEDQWNQDNFSFHDLEEVANRIGLFTGVILSFGISTNLDNSTRRIMEVDQPGLGLDREYLIKGFEDKDVQAYYSYMVDTAVYLGAQRKVAEQELKESLMFELKIAEITLPQEERRNTSALNNQMTIEEGNKLYPGF--NWTQHINNM---HNSLEVPLTSNEVINVAVPKYIIKLAE 325
BLAST of PREDICTED: neprilysin-2-like vs. nr
Match: gi|1067094761|ref|XP_018009276.1| (PREDICTED: neprilysin-like [Hyalella azteca]) HSP 1 Score: 464.151 bits (1193), Expect = 7.608e-140 Identity = 241/686 (35.13%), Postives = 381/686 (55.54%), Query Frame = 0
Query: 21 KMCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGG----------------------------------------------WPVLE----------ENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTH-NITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
+ CL+++C+ A+ L +M+ +DPCE+F+E+ CG +++ IP++KSS ++ + ++ LQ+K + L+E E S +D +++ Y+SC+N + + + +PL ++L +GG W +++ E L+ GL D V+AY Y + L+GA+R A+ EL + LLFE++LA S P E+RR+ ++LYN + + ++ +P P W S+++ L+ NITEDE V++ P + + L ++ K R +AN++ WR + + + DARD LEYSK +GT RWK C+ A S +A LYA+ +F K+AK+ +E++ VR F +I+ ++WMDD T+ +AI K A+ I YPDE+L+E K+ Y+ + + DG+ ++ +L S + +++LRE IDK W G A+VNA+Y N I FPAG+L+G FFN + P Y+N+G++G +T KF EK++CII Q NY + +VG+KLNG+NTQGENIADNGG+ +AY A+ ++++ +G + LP L T +QLFW+S VWC K R E LK LIL +H+P FRVNG LS + EF+RD+ C + S MNP +KC
Sbjct: 327 EFCLTKSCITSASELIANMDSQVDPCEDFFEYACGNYIKSRNIPDEKSSITQFSDVSDVLQEKLRMLIESEDSSEDTPSGLMVKQLYKSCMNTERIAEQGLQPLKDILREMGGWPAVEGAAWGHNRFDWVQNVYINRRLGYSVDYLLDFSVTTNIKNSTWRIIDIDQPSFGMSREYLMRGLNDSDVRAYLDYQVSLAALLGANRTAAQLELTESLLFEIQLANFSLPNEKRRNASELYNKMTVAELQARVPNIP--WLSYINTLLAPFTNITEDEEVIVNVPSYLENLDKLLLKTPKRVIANFLMWRASAASISYLSEDARDLQLEYSKKITGTGKRNPRWKECIGAVS------GSLSYAVSKLYAEKFFKKDAKAAADEMVSYVRREFDKILRAVDWMDDETRLRAINKSQAITSHIAYPDELLDESKIAEVYKDLQISDGELLSNMRNLTIYSADYSFKRLREVIDKNDWKRHGTAAVVNAYYSRVDNSINFPAGILQGTFFNAERPKYLNFGSIGFVIGHEITHGFDDVGRQFDYKGDLKEWWEPETKAKFIEKSKCIIHQYGNYTAPEVGLKLNGVNTQGENIADNGGIKEAYYAYNQYVSDHGEENPLPALGLTAKQLFWLSAANVWCGKYRPESLKFLILTGAHSPVRFRVNGELSNSPEFSRDWNCKLNSKMNPSQKC 1004 HSP 2 Score: 422.165 bits (1084), Expect = 2.479e-124 Identity = 205/462 (44.37%), Postives = 293/462 (63.42%), Query Frame = 0
Query: 894 GYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTH-NITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
GYS+D ++D + + KNSTWR I +DQP G+SREYL+ GL D V+AY Y + L+GA+R A++ EL + LLFE++LA S P E+RR+ ++LYN + + ++ +P P W S+++ L+ NITEDE V++ P + + L ++ K R +AN++ WR + + + DARD LEY+K +GT + RW+ C+ A S + LYA+ +F K+AK ++ VR F +IL +DWMDD T+ +A K A+ I +PDE+L+E K+ +YK +QI +G+ N+ +L S + ++RLRE +DK W T A+VNA YS N I FPAGIL G FFN + P Y N+G+IG VIGHEITHGFDD GR+ D GDL +WW+ +T KF +K++CII+Q N+ + +V LKLNG+NTQGENIADNGGI EAY AY
Sbjct: 466 GYSVDYLLDFSVTTNIKNSTWRIIDIDQPSFGMSREYLMRGLNDSDVRAYLDYQVSLAALLGANRTAAQLELTESLLFEIQLANFSLPNEKRRNASELYNKMTVAELQARVPNIP--WLSYINTLLAPFTNITEDEEVIVNVPSYLENLDKLLLKTPKRVIANFLMWRASAASISYLSEDARDLQLEYSKKITGTGKRNPRWKECIGAVS------GSLSYAVSKLYAEKFFKKDAKAAADEMVSYVRREFDKILRAVDWMDDETRLRAINKSQAITSHIAYPDELLDESKIAEVYKDLQISDGELLSNMRNLTIYSADYSFKRLREVIDKNDWKRHGTAAVVNAYYSRVDNSINFPAGILQGTFFNAERPKYLNFGSIGFVIGHEITHGFDDVGRQFDYKGDLKEWWEPETKAKFIEKSKCIIHQYGNYTAPEVGLKLNGVNTQGENIADNGGIKEAYYAY 919 HSP 3 Score: 226.483 bits (576), Expect = 6.711e-57 Identity = 112/316 (35.44%), Postives = 185/316 (58.54%), Query Frame = 0
Query: 583 DSHAPNEFRV------NGALSYNKEFARDFQCPIGSPMNPEEKCRSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTH-NITEDERVLIGNPEFFKKL 890
D P+E V G S EF C + E +M+ +DPCE+F+E+ CG +++ IP++KSS ++ + ++ LQ+K + L+E E S +D +++ Y+SC+N + + + +PL ++L +GGWP +EG W + W +N+Y + GYS+D ++D + + KNSTWR I +DQP G+SREYL+ GL D V+AY Y + L+GA+R A++ EL + LLFE++LA S P E+RR+ ++LYN + + ++ +P P W S+++ L+ NITEDE V++ P + + L
Sbjct: 303 DVSDPSELSVVSCKASGGQTSSTSEFCLTKSCITSA----SELIANMDSQVDPCEDFFEYACGNYIKSRNIPDEKSSITQFSDVSDVLQEKLRMLIESEDSSEDTPSGLMVKQLYKSCMNTERIAEQGLQPLKDILREMGGWPAVEGAAWGHNRFDWVQNVYINRRLGYSVDYLLDFSVTTNIKNSTWRIIDIDQPSFGMSREYLMRGLNDSDVRAYLDYQVSLAALLGANRTAAQLELTESLLFEIQLANFSLPNEKRRNASELYNKMTVAELQARVPNIP--WLSYINTLLAPFTNITEDEEVIVNVPSYLENL 612
BLAST of PREDICTED: neprilysin-2-like vs. nr
Match: gi|1209695928|gb|OWR48508.1| (zinc metalloprotease [Danaus plexippus plexippus]) HSP 1 Score: 454.521 bits (1168), Expect = 3.708e-139 Identity = 248/696 (35.63%), Postives = 372/696 (53.45%), Query Frame = 0
Query: 3 KVTPTMRIGNPGPGEEDFKMCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLE----------------------------------------------ENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTH-NITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGL-PFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCR 621
+++P+M P +D+ +C S C+ A+ L +M+ DPC++FY+F CG V+NT IP+DK+S + +I+ LQ++ + LL++ S+++ F + Y++C+N ++ +PL E+L LGGWPVL+ E L G D+ V+AY++YM DI L+GA+R + ELK+ L FEM+LA IS P E+RR+ LYNP + + + P P W ++++ L+ H + DE ++ P++ L D++ K R ANY+ WR + V T D R L Y SG +RWK C S A G+LY + YF + +KS E++ ++R+ F + + ++WMD+ T+ +A+EK AM I YP E+L+ DK+T Y G+ + K S L+L + + +LRE ++K W G A+VNAFY +N I FPAG+L+G FF+ PAY+NYGA+G T +K+ EKA+CIIDQ NY K+V +KLNG+NTQGENIADNGG+ +AY A++ ++G + LPGL ++ RQLFW+S WC R+E +K I HAP FRV G +S +EFA DF+CP GSPMNP +KC+
Sbjct: 73 RLSPSM---PPAVISKDYNLCTSPGCIHTASKLLINMDDKTDPCDDFYDFACGSFVKNTRIPDDKTSVNTFSIITDQLQEQIRALLDEPISENEPRPFVLAKTLYQACMNRTAIEARGVQPLLEMLLRLGGWPVLQGDTWNERSFSWEESVYRFREAGYSVDYFLDFSISVDLDQASLGLSREYLNRGFSDKLVQAYYEYMVDIATLLGAERAKAEVELKESLQFEMKLANISLPLEKRRNATSLYNPMTIAELQQKFPRIP--WLAYINRLLSPHVQVGLDEVTIVNVPKYITDLEDLLEKTPKRVQANYVMWRVAGASVSYLTEDLRRRQLAYVTALSGKTERESRWKECADTTSV------SMSIAVGALYIRKYFNENSKSNALEMVNDIRQQFRKTLETVDWMDEKTRREALEKADAMASHIAYPSEMLDNDKLTEFYSGLEMSSDKLMESVLNLTLFGTEYLFGKLREPVNKTDWVTHGRPAIVNAFYSSIENSIQFPAGILQGAFFSANRPAYMNYGAIGFVIGHEITHGFDDQGRQFDKNGNLVDWWQETTKQKYLEKAKCIIDQYFNYTVKEVNMKLNGVNTQGENIADNGGIKEAYYAYQAWTQRHGEEARLPGLEKYSARQLFWMSAANTWCSVYRNEAIKLRITTGFHAPGRFRVIGPMSNMEEFAADFKCPAGSPMNPVKKCK 757 HSP 2 Score: 391.349 bits (1004), Expect = 8.914e-116 Identity = 198/467 (42.40%), Postives = 281/467 (60.17%), Query Frame = 0
Query: 889 KLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTH-NITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
+ + GYS+D +D I ++ LDQ LGLSREYL G D+ V+AY++YM DI L+GA+R +E ELK+ L FEM+LA IS P E+RR+ LYNP + + + P P W ++++ L+ H + DE ++ P++ L D++ K R ANY+ WR + V T D R L Y SG E +RW+ C T S G+LY + YF + +K ++ ++R F++ L+ +DWMD+ T+ +A EK AM I +P E+L+ DK+T Y G+++ + +++L+L ++ + +LRE V+K W A+VNA YS +N I FPAGIL G FF+ + P Y NYGAIG VIGHEITHGFDDQGR+ D+NG+L DWW E T QK+ +KA+CII+Q N+ +V +KLNG+NTQGENIADNGGI EAY AY
Sbjct: 222 RFREAGYSVDYFLDFSI----------SVDLDQASLGLSREYLNRGFSDKLVQAYYEYMVDIATLLGAERAKAEVELKESLQFEMKLANISLPLEKRRNATSLYNPMTIAELQQKFPRIP--WLAYINRLLSPHVQVGLDEVTIVNVPKYITDLEDLLEKTPKRVQANYVMWRVAGASVSYLTEDLRRRQLAYVTALSGKTERESRWKECADTTSV------SMSIAVGALYIRKYFNENSKSNALEMVNDIRQQFRKTLETVDWMDEKTRREALEKADAMASHIAYPSEMLDNDKLTEFYSGLEMSSDKLMESVLNLTLFGTEYLFGKLREPVNKTDWVTHGRPAIVNAFYSSIENSIQFPAGILQGAFFSANRPAYMNYGAIGFVIGHEITHGFDDQGRQFDKNGNLVDWWQETTKQKYLEKAKCIIDQYFNYTVKEVNMKLNGVNTQGENIADNGGIKEAYYAY 670 HSP 3 Score: 207.608 bits (527), Expect = 8.113e-52 Identity = 124/358 (34.64%), Postives = 191/358 (53.35%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTH-NITEDERVLIGNPEFFKKLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMG-ADRNASEAELKDVLLFEMELA 976
+M+ DPC++FY+F CG V+NT IP+DK+S + +I+ LQ++ + LL++ S+++ F + Y++C+N ++ +PL E+L LGGWPVL+G W+ + W E++Y + GYS+D +D I ++ LDQ LGLSREYL G D+ V+AY++YM DI L+GA+R +E ELK+ L FEM+LA IS P E+RR+ LYNP + + + P P W ++++ L+ H + DE ++ P++ L D V A WR V G S YL E L + + AY +T L G +R + E D M +A
Sbjct: 105 NMDDKTDPCDDFYDFACGSFVKNTRIPDDKTSVNTFSIITDQLQEQIRALLDEPISENEPRPFVLAKTLYQACMNRTAIEARGVQPLLEMLLRLGGWPVLQGDTWNERSFSWEESVYRFREAGYSVDYFLDFSI----------SVDLDQASLGLSREYLNRGFSDKLVQAYYEYMVDIATLLGAERAKAEVELKESLQFEMKLANISLPLEKRRNATSLYNPMTIAELQQKFPRIP--WLAYINRLLSPHVQVGLDEVTIVNVPKYITDLEDLLEKTPKRV------QANYVMWR-------VAGASVSYLTEDLRRRQL-AY------VTALSGKTERESRWKECADTTSVSMSIA 430
BLAST of PREDICTED: neprilysin-2-like vs. nr
Match: gi|972184594|ref|XP_015173767.1| (PREDICTED: membrane metallo-endopeptidase-like 1 isoform X3 [Polistes dominula]) HSP 1 Score: 454.136 bits (1167), Expect = 6.081e-139 Identity = 239/688 (34.74%), Postives = 373/688 (54.22%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEE--------NLIEGLEDQHVKAY------------------------------------------------FKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLL----KDFESLPGHPPSWTSFVDNLVY-THNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
+C+S CV A+ + ++M+ ++PC++FY+F CG +++T+IP+DK+S + +I++ +LQQ+ + +E++ ++ + F + FY++C+N+ +++ EPL +L TLGGWPVLE N I+ + K Y + YM DI ++GAD+N + ELK+ L FEM+LA IS P E+RR+ LYNP + K+F S+P W + + ++ T + E+E V++ P + L ++++K R ANY+ WR V T + R L+YS SG ARWK CV S + G++Y + YF ++AK E++ ++RE F +I+ ++WMD+ TK A++K +M I YPDE+L++ K+ Y+ + + DG Y S L+L + + +LR+ ++K W G A+VNAFY +N I FPAG+L+G FFN+ P Y+NYGA+G +T + + E+A+CI+ Q NY ++VG+ LNGINTQGENIADNGG+ +AY A+++ + +N +P LPGL +TP Q+FWIS WC K R E +K I H+P EFRV G S EF++DF CP+GS MNPE+KC
Sbjct: 81 ICVSPGCVHTASKILENMDSDVEPCDDFYQFACGGFLKSTIIPDDKTSVNTFSIISDNLQQQLRTSIEEKSPPNEPKPFTLAKIFYKTCMNKTAIEERGLEPLHNILKTLGGWPVLEGDNWKEEDFNWIDSVYKFRTKGYSVDYFIDFSIGVDLKNSTMRIIDLDQASLGLSREYLSKGFDDKIVQAYYDYMVDIAVILGADKNRAKTELKESLEFEMKLANISLPNEKRRNATLLYNPMTITELNKNFPSIP-----WKEYFNTILAPTVQVDENEVVIVSVPSYITSLEELLSKTPKRIQANYVMWRAAAGSVSYLTENIRRRQLQYSTALSGKTEREARWKECVDIVS------GSLSISVGAMYVRKYFKEDAKKNAIEMVSDIREEFKKILKKVDWMDENTKESALDKAESMSSHIAYPDELLDDKKLEEFYKKLELTDGSYLESILNLTLFGTEYSFSKLRKPVNKSDWVTHGRPAIVNAFYSSIENSIQFPAGILQGTFFNNDRPRYMNYGAIGFVIGHEITHGFDDQGRQFDKEGNLVDWWAPETKENYLERAECIVHQYGNYTVEEVGLNLNGINTQGENIADNGGIKEAYFAYKEWVKRNHPEPKLPGLSYTPEQMFWISAANTWCSKYRPEAMKLRITTGFHSPGEFRVLGPFSNMNEFSKDFNCPLGSKMNPEKKC 757 HSP 2 Score: 407.142 bits (1045), Expect = 1.750e-121 Identity = 204/470 (43.40%), Postives = 290/470 (61.70%), Query Frame = 0
Query: 889 KLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLL----KDFESLPGHPPSWTSFVDNLVY-THNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
K +GYS+D +D I D KNST R I LDQ LGLSREYL +G +D+ V+AY+ YM DI ++GAD+N ++ ELK+ L FEM+LA IS P E+RR+ LYNP + K+F S+P W + + ++ T + E+E V++ P + L ++++K R ANY+ WR V T + R L+Y+ SG E ARW+ CV S G++Y + YF ++AK ++ ++R FK+IL +DWMD+ TK A +K +M I +PDE+L++ K+ YK +++ +G + ++IL+L ++ + +LR+ V+K W A+VNA YS +N I FPAGIL G FFN D P Y NYGAIG VIGHEITHGFDDQGR+ D+ G+L DWW +T + + ++A+CI++Q N+ +V L LNGINTQGENIADNGGI EAY AY
Sbjct: 214 KFRTKGYSVDYFIDFSIGVDLKNSTMRIIDLDQASLGLSREYLSKGFDDKIVQAYYDYMVDIAVILGADKNRAKTELKESLEFEMKLANISLPNEKRRNATLLYNPMTITELNKNFPSIP-----WKEYFNTILAPTVQVDENEVVIVSVPSYITSLEELLSKTPKRIQANYVMWRAAAGSVSYLTENIRRRQLQYSTALSGKTEREARWKECVDIVS------GSLSISVGAMYVRKYFKEDAKKNAIEMVSDIREEFKKILKKVDWMDENTKESALDKAESMSSHIAYPDELLDDKKLEEFYKKLELTDGSYLESILNLTLFGTEYSFSKLRKPVNKSDWVTHGRPAIVNAFYSSIENSIQFPAGILQGTFFNNDRPRYMNYGAIGFVIGHEITHGFDDQGRQFDKEGNLVDWWAPETKENYLERAECIVHQYGNYTVEEVGLNLNGINTQGENIADNGGIKEAYFAY 672 HSP 3 Score: 229.18 bits (583), Expect = 5.525e-59 Identity = 110/276 (39.86%), Postives = 179/276 (64.86%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLL----KDFESLPGHPPSWTSFVDNLVY-THNITEDERVLIGNPEFFKKLSD 892
+M+ ++PC++FY+F CG +++T+IP+DK+S + +I++ +LQQ+ + +E++ ++ + F + FY++C+N+ +++ EPL +L TLGGWPVLEG NW D+ W +++Y +GYS+D +D I D KNST R I LDQ LGLSREYL +G +D+ V+AY+ YM DI ++GAD+N ++ ELK+ L FEM+LA IS P E+RR+ LYNP + K+F S+P W + + ++ T + E+E V++ P + L +
Sbjct: 97 NMDSDVEPCDDFYQFACGGFLKSTIIPDDKTSVNTFSIISDNLQQQLRTSIEEKSPPNEPKPFTLAKIFYKTCMNKTAIEERGLEPLHNILKTLGGWPVLEGDNWKEEDFNWIDSVYKFRTKGYSVDYFIDFSIGVDLKNSTMRIIDLDQASLGLSREYLSKGFDDKIVQAYYDYMVDIAVILGADKNRAKTELKESLEFEMKLANISLPNEKRRNATLLYNPMTITELNKNFPSIP-----WKEYFNTILAPTVQVDENEVVIVSVPSYITSLEE 367
BLAST of PREDICTED: neprilysin-2-like vs. nr
Match: gi|972184592|ref|XP_015173766.1| (PREDICTED: neprilysin isoform X2 [Polistes dominula]) HSP 1 Score: 454.136 bits (1167), Expect = 6.247e-139 Identity = 239/688 (34.74%), Postives = 373/688 (54.22%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEE--------NLIEGLEDQHVKAY------------------------------------------------FKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLL----KDFESLPGHPPSWTSFVDNLVY-THNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
+C+S CV A+ + ++M+ ++PC++FY+F CG +++T+IP+DK+S + +I++ +LQQ+ + +E++ ++ + F + FY++C+N+ +++ EPL +L TLGGWPVLE N I+ + K Y + YM DI ++GAD+N + ELK+ L FEM+LA IS P E+RR+ LYNP + K+F S+P W + + ++ T + E+E V++ P + L ++++K R ANY+ WR V T + R L+YS SG ARWK CV S + G++Y + YF ++AK E++ ++RE F +I+ ++WMD+ TK A++K +M I YPDE+L++ K+ Y+ + + DG Y S L+L + + +LR+ ++K W G A+VNAFY +N I FPAG+L+G FFN+ P Y+NYGA+G +T + + E+A+CI+ Q NY ++VG+ LNGINTQGENIADNGG+ +AY A+++ + +N +P LPGL +TP Q+FWIS WC K R E +K I H+P EFRV G S EF++DF CP+GS MNPE+KC
Sbjct: 87 ICVSPGCVHTASKILENMDSDVEPCDDFYQFACGGFLKSTIIPDDKTSVNTFSIISDNLQQQLRTSIEEKSPPNEPKPFTLAKIFYKTCMNKTAIEERGLEPLHNILKTLGGWPVLEGDNWKEEDFNWIDSVYKFRTKGYSVDYFIDFSIGVDLKNSTMRIIDLDQASLGLSREYLSKGFDDKIVQAYYDYMVDIAVILGADKNRAKTELKESLEFEMKLANISLPNEKRRNATLLYNPMTITELNKNFPSIP-----WKEYFNTILAPTVQVDENEVVIVSVPSYITSLEELLSKTPKRIQANYVMWRAAAGSVSYLTENIRRRQLQYSTALSGKTEREARWKECVDIVS------GSLSISVGAMYVRKYFKEDAKKNAIEMVSDIREEFKKILKKVDWMDENTKESALDKAESMSSHIAYPDELLDDKKLEEFYKKLELTDGSYLESILNLTLFGTEYSFSKLRKPVNKSDWVTHGRPAIVNAFYSSIENSIQFPAGILQGTFFNNDRPRYMNYGAIGFVIGHEITHGFDDQGRQFDKEGNLVDWWAPETKENYLERAECIVHQYGNYTVEEVGLNLNGINTQGENIADNGGIKEAYFAYKEWVKRNHPEPKLPGLSYTPEQMFWISAANTWCSKYRPEAMKLRITTGFHSPGEFRVLGPFSNMNEFSKDFNCPLGSKMNPEKKC 763 HSP 2 Score: 407.142 bits (1045), Expect = 2.036e-121 Identity = 204/470 (43.40%), Postives = 290/470 (61.70%), Query Frame = 0
Query: 889 KLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLL----KDFESLPGHPPSWTSFVDNLVY-THNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
K +GYS+D +D I D KNST R I LDQ LGLSREYL +G +D+ V+AY+ YM DI ++GAD+N ++ ELK+ L FEM+LA IS P E+RR+ LYNP + K+F S+P W + + ++ T + E+E V++ P + L ++++K R ANY+ WR V T + R L+Y+ SG E ARW+ CV S G++Y + YF ++AK ++ ++R FK+IL +DWMD+ TK A +K +M I +PDE+L++ K+ YK +++ +G + ++IL+L ++ + +LR+ V+K W A+VNA YS +N I FPAGIL G FFN D P Y NYGAIG VIGHEITHGFDDQGR+ D+ G+L DWW +T + + ++A+CI++Q N+ +V L LNGINTQGENIADNGGI EAY AY
Sbjct: 220 KFRTKGYSVDYFIDFSIGVDLKNSTMRIIDLDQASLGLSREYLSKGFDDKIVQAYYDYMVDIAVILGADKNRAKTELKESLEFEMKLANISLPNEKRRNATLLYNPMTITELNKNFPSIP-----WKEYFNTILAPTVQVDENEVVIVSVPSYITSLEELLSKTPKRIQANYVMWRAAAGSVSYLTENIRRRQLQYSTALSGKTEREARWKECVDIVS------GSLSISVGAMYVRKYFKEDAKKNAIEMVSDIREEFKKILKKVDWMDENTKESALDKAESMSSHIAYPDELLDDKKLEEFYKKLELTDGSYLESILNLTLFGTEYSFSKLRKPVNKSDWVTHGRPAIVNAFYSSIENSIQFPAGILQGTFFNNDRPRYMNYGAIGFVIGHEITHGFDDQGRQFDKEGNLVDWWAPETKENYLERAECIVHQYGNYTVEEVGLNLNGINTQGENIADNGGIKEAYFAY 678 HSP 3 Score: 229.18 bits (583), Expect = 6.397e-59 Identity = 110/276 (39.86%), Postives = 179/276 (64.86%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLL----KDFESLPGHPPSWTSFVDNLVY-THNITEDERVLIGNPEFFKKLSD 892
+M+ ++PC++FY+F CG +++T+IP+DK+S + +I++ +LQQ+ + +E++ ++ + F + FY++C+N+ +++ EPL +L TLGGWPVLEG NW D+ W +++Y +GYS+D +D I D KNST R I LDQ LGLSREYL +G +D+ V+AY+ YM DI ++GAD+N ++ ELK+ L FEM+LA IS P E+RR+ LYNP + K+F S+P W + + ++ T + E+E V++ P + L +
Sbjct: 103 NMDSDVEPCDDFYQFACGGFLKSTIIPDDKTSVNTFSIISDNLQQQLRTSIEEKSPPNEPKPFTLAKIFYKTCMNKTAIEERGLEPLHNILKTLGGWPVLEGDNWKEEDFNWIDSVYKFRTKGYSVDYFIDFSIGVDLKNSTMRIIDLDQASLGLSREYLSKGFDDKIVQAYYDYMVDIAVILGADKNRAKTELKESLEFEMKLANISLPNEKRRNATLLYNPMTITELNKNFPSIP-----WKEYFNTILAPTVQVDENEVVIVSVPSYITSLEE 373
BLAST of PREDICTED: neprilysin-2-like vs. nr
Match: gi|972184590|ref|XP_015173765.1| (PREDICTED: neprilysin isoform X1 [Polistes dominula]) HSP 1 Score: 454.136 bits (1167), Expect = 7.666e-139 Identity = 239/688 (34.74%), Postives = 373/688 (54.22%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEE--------NLIEGLEDQHVKAY------------------------------------------------FKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLL----KDFESLPGHPPSWTSFVDNLVY-THNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
+C+S CV A+ + ++M+ ++PC++FY+F CG +++T+IP+DK+S + +I++ +LQQ+ + +E++ ++ + F + FY++C+N+ +++ EPL +L TLGGWPVLE N I+ + K Y + YM DI ++GAD+N + ELK+ L FEM+LA IS P E+RR+ LYNP + K+F S+P W + + ++ T + E+E V++ P + L ++++K R ANY+ WR V T + R L+YS SG ARWK CV S + G++Y + YF ++AK E++ ++RE F +I+ ++WMD+ TK A++K +M I YPDE+L++ K+ Y+ + + DG Y S L+L + + +LR+ ++K W G A+VNAFY +N I FPAG+L+G FFN+ P Y+NYGA+G +T + + E+A+CI+ Q NY ++VG+ LNGINTQGENIADNGG+ +AY A+++ + +N +P LPGL +TP Q+FWIS WC K R E +K I H+P EFRV G S EF++DF CP+GS MNPE+KC
Sbjct: 92 ICVSPGCVHTASKILENMDSDVEPCDDFYQFACGGFLKSTIIPDDKTSVNTFSIISDNLQQQLRTSIEEKSPPNEPKPFTLAKIFYKTCMNKTAIEERGLEPLHNILKTLGGWPVLEGDNWKEEDFNWIDSVYKFRTKGYSVDYFIDFSIGVDLKNSTMRIIDLDQASLGLSREYLSKGFDDKIVQAYYDYMVDIAVILGADKNRAKTELKESLEFEMKLANISLPNEKRRNATLLYNPMTITELNKNFPSIP-----WKEYFNTILAPTVQVDENEVVIVSVPSYITSLEELLSKTPKRIQANYVMWRAAAGSVSYLTENIRRRQLQYSTALSGKTEREARWKECVDIVS------GSLSISVGAMYVRKYFKEDAKKNAIEMVSDIREEFKKILKKVDWMDENTKESALDKAESMSSHIAYPDELLDDKKLEEFYKKLELTDGSYLESILNLTLFGTEYSFSKLRKPVNKSDWVTHGRPAIVNAFYSSIENSIQFPAGILQGTFFNNDRPRYMNYGAIGFVIGHEITHGFDDQGRQFDKEGNLVDWWAPETKENYLERAECIVHQYGNYTVEEVGLNLNGINTQGENIADNGGIKEAYFAYKEWVKRNHPEPKLPGLSYTPEQMFWISAANTWCSKYRPEAMKLRITTGFHSPGEFRVLGPFSNMNEFSKDFNCPLGSKMNPEKKC 768 HSP 2 Score: 407.142 bits (1045), Expect = 2.352e-121 Identity = 204/470 (43.40%), Postives = 290/470 (61.70%), Query Frame = 0
Query: 889 KLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLL----KDFESLPGHPPSWTSFVDNLVY-THNITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
K +GYS+D +D I D KNST R I LDQ LGLSREYL +G +D+ V+AY+ YM DI ++GAD+N ++ ELK+ L FEM+LA IS P E+RR+ LYNP + K+F S+P W + + ++ T + E+E V++ P + L ++++K R ANY+ WR V T + R L+Y+ SG E ARW+ CV S G++Y + YF ++AK ++ ++R FK+IL +DWMD+ TK A +K +M I +PDE+L++ K+ YK +++ +G + ++IL+L ++ + +LR+ V+K W A+VNA YS +N I FPAGIL G FFN D P Y NYGAIG VIGHEITHGFDDQGR+ D+ G+L DWW +T + + ++A+CI++Q N+ +V L LNGINTQGENIADNGGI EAY AY
Sbjct: 225 KFRTKGYSVDYFIDFSIGVDLKNSTMRIIDLDQASLGLSREYLSKGFDDKIVQAYYDYMVDIAVILGADKNRAKTELKESLEFEMKLANISLPNEKRRNATLLYNPMTITELNKNFPSIP-----WKEYFNTILAPTVQVDENEVVIVSVPSYITSLEELLSKTPKRIQANYVMWRAAAGSVSYLTENIRRRQLQYSTALSGKTEREARWKECVDIVS------GSLSISVGAMYVRKYFKEDAKKNAIEMVSDIREEFKKILKKVDWMDENTKESALDKAESMSSHIAYPDELLDDKKLEEFYKKLELTDGSYLESILNLTLFGTEYSFSKLRKPVNKSDWVTHGRPAIVNAFYSSIENSIQFPAGILQGTFFNNDRPRYMNYGAIGFVIGHEITHGFDDQGRQFDKEGNLVDWWAPETKENYLERAECIVHQYGNYTVEEVGLNLNGINTQGENIADNGGIKEAYFAY 683 HSP 3 Score: 229.565 bits (584), Expect = 5.951e-59 Identity = 110/276 (39.86%), Postives = 179/276 (64.86%), Query Frame = 0
Query: 622 SMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLL----KDFESLPGHPPSWTSFVDNLVY-THNITEDERVLIGNPEFFKKLSD 892
+M+ ++PC++FY+F CG +++T+IP+DK+S + +I++ +LQQ+ + +E++ ++ + F + FY++C+N+ +++ EPL +L TLGGWPVLEG NW D+ W +++Y +GYS+D +D I D KNST R I LDQ LGLSREYL +G +D+ V+AY+ YM DI ++GAD+N ++ ELK+ L FEM+LA IS P E+RR+ LYNP + K+F S+P W + + ++ T + E+E V++ P + L +
Sbjct: 108 NMDSDVEPCDDFYQFACGGFLKSTIIPDDKTSVNTFSIISDNLQQQLRTSIEEKSPPNEPKPFTLAKIFYKTCMNKTAIEERGLEPLHNILKTLGGWPVLEGDNWKEEDFNWIDSVYKFRTKGYSVDYFIDFSIGVDLKNSTMRIIDLDQASLGLSREYLSKGFDDKIVQAYYDYMVDIAVILGADKNRAKTELKESLEFEMKLANISLPNEKRRNATLLYNPMTITELNKNFPSIP-----WKEYFNTILAPTVQVDENEVVIVSVPSYITSLEE 378
BLAST of PREDICTED: neprilysin-2-like vs. nr
Match: gi|662208230|ref|XP_008477654.1| (PREDICTED: neprilysin-11-like [Diaphorina citri]) HSP 1 Score: 454.136 bits (1167), Expect = 9.429e-139 Identity = 240/693 (34.63%), Postives = 383/693 (55.27%), Query Frame = 0
Query: 16 GEEDFKMCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLE-----------------------------------------ENLIE------GLEDQHV---------KAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLP-FTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKCR 621
G+ K+C +E CV A+ L ++M+ ++DPC++FY F+CG+ ++ T IP+DK++ + ++++ L+++ + ++E+ +++ + FK ++ Y++C+N ++ + EP+ +L LGGWPVLE E +I+ GL +++ AY++YM DI L+GAD++ + ELK+ L FE++LA IS P E+RR+ KLYNP L + ++ P P W +++ L+Y + + E + V++ P + KL +++ + ANY WR + V T R+ L+YS SG + RWK CV SS A G+LY + +F ++AK+ E++E +RE +I+N +EWMD+ T+ KA+EK AM I YPDE+L+++K++ Y+ + ++ Y+ S L+L + + + LR+ ++K W G A+VNAFY +N I FPAG+L+G+FF++ P Y+N+GA+G DT + + EKA+CII+Q NY +V + LNG+N QGENIADNGG+ +AY A+ A + +P LPGL FTP+Q+FWIS WC K R E LK I H+P EFRV G +S + F+RDFQCP GS MNP KC+
Sbjct: 89 GDNKPKICFTEGCVHTASNLLKNMDLNVDPCDDFYRFSCGKFLKETQIPDDKTAQTAFSVISDALEEQLRTIVEEPIRENEPKPFKLVKNLYKACMNVKNIEILGLEPMKRMLQDLGGWPVLEGQNWNEETFSWKDSVYKFHENGYSVDYFIDFSIVINVKNNSERIIDLDQATLGLSREYLVKGINEKLVNAYYRYMIDIAVLLGADKDVATEELKESLEFEIKLANISLPLEQRRNATKLYNPMSLDELQTKFPSIP--WVEYLNTLLYPKDRLRETDIVVVDVPNYISKLEELLQTTPKKVQANYALWRATAASVSYLTEAIRNRQLQYSTALSGRTQMEPRWKECVDTVS------SSISLAMGALYVRKFFKEDAKANAIEMVELIREEMNKILNTVEWMDNKTRRKALEKAKAMTAHIAYPDELLDDNKISEFYDKLEIDPNNYYKSILNLTKFGTGYSFSLLRKPVNKTEWISHGHPALVNAFYSSIENSITFPAGILQGSFFSNDRPKYMNFGAIGFVIGHEITHGFDDQGRQFDKNGNLVDWWQEDTKRSYIEKARCIIEQYGNYSVPEVNLTLNGVNNQGENIADNGGMKEAYNAYVNWAATHPAEPRLPGLQNFTPQQMFWISAASTWCSKHRPETLKLRITTGFHSPGEFRVLGPVSNLESFSRDFQCPSGSRMNPVHKCK 773 HSP 2 Score: 397.127 bits (1019), Expect = 1.150e-117 Identity = 194/468 (41.45%), Postives = 289/468 (61.75%), Query Frame = 0
Query: 889 KLSDEGYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAYA 1354
K + GYS+D +D I + KN++ R I LDQ LGLSREYL++G+ ++ V AY++YM DI L+GAD++ + ELK+ L FE++LA IS P E+RR+ KLYNP L + ++ P P W +++ L+Y + + E + V++ P + KL +++ + ANY WR + V T R+ L+Y+ SG ++ RW+ CV +S G+LY + +F ++AK ++ +R +IL+ ++WMD+ T+ KA EK AM I +PDE+L+++K++ Y ++I ++++IL+L + + + LR+ V+K W A+VNA YS +N I FPAGIL G FF+ D P Y N+GAIG VIGHEITHGFDDQGR+ D+NG+L DWW EDT + + +KA+CII Q N+ +V L LNG+N QGENIADNGG+ EAY AY
Sbjct: 228 KFHENGYSVDYFIDFSIVINVKNNSERIIDLDQATLGLSREYLVKGINEKLVNAYYRYMIDIAVLLGADKDVATEELKESLEFEIKLANISLPLEQRRNATKLYNPMSLDELQTKFPSIP--WVEYLNTLLYPKDRLRETDIVVVDVPNYISKLEELLQTTPKKVQANYALWRATAASVSYLTEAIRNRQLQYSTALSGRTQMEPRWKECVDTVS------SSISLAMGALYVRKFFKEDAKANAIEMVELIREEMNKILNTVEWMDNKTRRKALEKAKAMTAHIAYPDELLDDNKISEFYDKLEIDPNNYYKSILNLTKFGTGYSFSLLRKPVNKTEWISHGHPALVNAFYSSIENSITFPAGILQGSFFSNDRPKYMNFGAIGFVIGHEITHGFDDQGRQFDKNGNLVDWWQEDTKRSYIEKARCIIEQYGNYSVPEVNLTLNGVNNQGENIADNGGMKEAYNAYV 687 HSP 3 Score: 220.705 bits (561), Expect = 4.270e-56 Identity = 100/274 (36.50%), Postives = 183/274 (66.79%), Query Frame = 0
Query: 621 RSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGLSREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSD 892
++M+ ++DPC++FY F+CG+ ++ T IP+DK++ + ++++ L+++ + ++E+ +++ + FK ++ Y++C+N ++ + EP+ +L LGGWPVLEG+NW+ + W +++Y ++ GYS+D +D I + KN++ R I LDQ LGLSREYL++G+ ++ V AY++YM DI L+GAD++ + ELK+ L FE++LA IS P E+RR+ KLYNP L + ++ P P W +++ L+Y + + E + V++ P + KL +
Sbjct: 110 KNMDLNVDPCDDFYRFSCGKFLKETQIPDDKTAQTAFSVISDALEEQLRTIVEEPIRENEPKPFKLVKNLYKACMNVKNIEILGLEPMKRMLQDLGGWPVLEGQNWNEETFSWKDSVYKFHENGYSVDYFIDFSIVINVKNNSERIIDLDQATLGLSREYLVKGINEKLVNAYYRYMIDIAVLLGADKDVATEELKESLEFEIKLANISLPLEQRRNATKLYNPMSLDELQTKFPSIP--WVEYLNTLLYPKDRLRETDIVVVDVPNYISKLEE 381
BLAST of PREDICTED: neprilysin-2-like vs. nr
Match: gi|1067094767|ref|XP_018009279.1| (PREDICTED: neprilysin-like isoform X2 [Hyalella azteca]) HSP 1 Score: 453.366 bits (1165), Expect = 1.471e-138 Identity = 240/686 (34.99%), Postives = 375/686 (54.66%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGG----------------------------------------------WPVLE-----------ENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
+CL++ CV A+ L ++M+ MDPCE+F+E+ CG ++ IP++KSS S+ + ++ LQ+K + L+E E S +D +++ Y+SC+N + + + +PL ++L +GG W +++ E L+ GL D V+AY Y + L+GA+R A+ EL + LLFE++LA S PKE+RR+ KLYN + + + +P P W S+++ ++ +TE E V++ P + + L ++ + R +ANY+ WR + + + DARD L YSK +GT RWK C+ A S +A G LYA+ +F K+AK+ +E++ +R F +I+ ++WMDD T+ +AI K +A+ I YP+E+L+E K+T Y+ + + DG+ ++ +L + +++LRE++DK W + G A+VNAFY +N I FPAG+L+G FFN P Y+NYGA+G +T KF +K++CII Q NY +VG+KLNG+NTQGENIADNGG+ +AY A+ ++++ +G + LP L T +QLFW+S VWC K R E LK IL +H+P FRVNG LS EF+RD+ C S MNP KC
Sbjct: 83 ICLTKGCVTSASELIENMDPQMDPCEDFFEYACGNYLKTKNIPDEKSSISQFSDVSDVLQEKLRTLVESEDSSEDTPSSLMVKQLYKSCMNTERIAEQGLQPLKDILREMGGWPAVEGFAWDRNRFDWVQNVYINRRLGYSVDLLFDFSITTNIKNSSWRIIDIDQPSLGMPSREYLLRGLNDSDVRAYLDYQVSLAALLGANRTAAELELTESLLFEIQLANFSLPKEQRRNATKLYNKMTVAELQGRVPNIP--WLSYINTILSPFTTVTEREEVIVNVPSYIENLGKLLLRTDKRVIANYMMWRLSAASISYLSEDARDLQLVYSKKITGTGKRKPRWKECMGAVS------GSLSYAVGKLYAEKFFKKDAKAAADEMVSYIRAEFDKILRTVDWMDDETRIRAINKSLAITPHIAYPEELLDETKLTELYKDLQISDGELLSNMRNLTIFGTDYSFKRLREKVDKNDWKKHGAAAVVNAFYSPLENSIQFPAGILQGTFFNADRPKYLNYGAIGFVIGHEITHGFDDMGRQFDYKGDLKEWWKPETRAKFIDKSKCIIYQYGNYTEPEVGLKLNGVNTQGENIADNGGIKEAYYAYNQYVSDHGEENPLPALGLTAKQLFWLSAANVWCGKYRPETLKLRILTGAHSPARFRVNGPLSNLPEFSRDWNCKSSSKMNPSHKC 760 HSP 2 Score: 423.705 bits (1088), Expect = 1.515e-127 Identity = 209/463 (45.14%), Postives = 292/463 (63.07%), Query Frame = 0
Query: 894 GYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
GYS+D++ D I + KNS+WR I +DQP LG+ SREYL+ GL D V+AY Y + L+GA+R A+E EL + LLFE++LA S PKE+RR+ KLYN + + + +P P W S+++ ++ +TE E V++ P + + L ++ + R +ANY+ WR + + + DARD L Y+K +GT + RW+ C+ A S + G LYA+ +F K+AK ++ +RA F +IL +DWMDD T+ +A K A+ I +P+E+L+E K+T +YK +QI +G+ N+ +L + ++RLRE+VDK W + A+VNA YSP +N I FPAGIL G FFN D P Y NYGAIG VIGHEITHGFDD GR+ D GDL +WW +T KF DK++CII Q N+ +V LKLNG+NTQGENIADNGGI EAY AY
Sbjct: 221 GYSVDLLFDFSITTNIKNSSWRIIDIDQPSLGMPSREYLLRGLNDSDVRAYLDYQVSLAALLGANRTAAELELTESLLFEIQLANFSLPKEQRRNATKLYNKMTVAELQGRVPNIP--WLSYINTILSPFTTVTEREEVIVNVPSYIENLGKLLLRTDKRVIANYMMWRLSAASISYLSEDARDLQLVYSKKITGTGKRKPRWKECMGAVS------GSLSYAVGKLYAEKFFKKDAKAAADEMVSYIRAEFDKILRTVDWMDDETRIRAINKSLAITPHIAYPEELLDETKLTELYKDLQISDGELLSNMRNLTIFGTDYSFKRLREKVDKNDWKKHGAAAVVNAFYSPLENSIQFPAGILQGTFFNADRPKYLNYGAIGFVIGHEITHGFDDMGRQFDYKGDLKEWWKPETRAKFIDKSKCIIYQYGNYTEPEVGLKLNGVNTQGENIADNGGIKEAYYAY 675 HSP 3 Score: 223.016 bits (567), Expect = 6.891e-57 Identity = 108/283 (38.16%), Postives = 175/283 (61.84%), Query Frame = 0
Query: 611 GSPMNPEEKCRSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTH-NITEDERVLIGNPEFFKKL 890
G + E +M+ MDPCE+F+E+ CG ++ IP++KSS S+ + ++ LQ+K + L+E E S +D +++ Y+SC+N + + + +PL ++L +GGWP +EG WDR + W +N+Y + GYS+D++ D I + KNS+WR I +DQP LG+ SREYL+ GL D V+AY Y + L+GA+R A+E EL + LLFE++LA S PKE+RR+ KLYN + + + +P P W S+++ ++ +TE E V++ P + + L
Sbjct: 88 GCVTSASELIENMDPQMDPCEDFFEYACGNYLKTKNIPDEKSSISQFSDVSDVLQEKLRTLVESEDSSEDTPSSLMVKQLYKSCMNTERIAEQGLQPLKDILREMGGWPAVEGFAWDRNRFDWVQNVYINRRLGYSVDLLFDFSITTNIKNSSWRIIDIDQPSLGMPSREYLLRGLNDSDVRAYLDYQVSLAALLGANRTAAELELTESLLFEIQLANFSLPKEQRRNATKLYNKMTVAELQGRVPNIP--WLSYINTILSPFTTVTEREEVIVNVPSYIENL 368
BLAST of PREDICTED: neprilysin-2-like vs. nr
Match: gi|1067094765|ref|XP_018009278.1| (PREDICTED: neprilysin-like isoform X1 [Hyalella azteca]) HSP 1 Score: 455.292 bits (1170), Expect = 1.063e-136 Identity = 240/686 (34.99%), Postives = 375/686 (54.66%), Query Frame = 0
Query: 22 MCLSEACVGMANGLFQSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGG----------------------------------------------WPVLE-----------ENLIEGLEDQHVKAYFKYMQDITELMGADRNASHAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYSKVTSGTQALPARWKTCVSAAGFGTFSESSFIFAAGSLYAKHYFPKEAKSIMNELIENVRESFTEIINDIEWMDDVTKGKAIEKLVAMDQMIGYPDEILEEDKVTSRYEGVVVEDGKYFASFLSLRRRSRTFGYEQLREEIDKKAWHELGIVAMVNAFYDWSKNMIFFPAGVLRGNFFNHKAPAYVNYGAVGMA----------------------------DTSKKFEEKAQCIIDQANNYESKQVGVKLNGINTQGENIADNGGLAQAYKAFEKHLAKNGMDPMLPGLPFTPRQLFWISFGRVWCKKSRDEHLKTLILLDSHAPNEFRVNGALSYNKEFARDFQCPIGSPMNPEEKC 620
+CL++ CV A+ L ++M+ MDPCE+F+E+ CG ++ IP++KSS S+ + ++ LQ+K + L+E E S +D +++ Y+SC+N + + + +PL ++L +GG W +++ E L+ GL D V+AY Y + L+GA+R A+ EL + LLFE++LA S PKE+RR+ KLYN + + + +P P W S+++ ++ +TE E V++ P + + L ++ + R +ANY+ WR + + + DARD L YSK +GT RWK C+ A S +A G LYA+ +F K+AK+ +E++ +R F +I+ ++WMDD T+ +AI K +A+ I YP+E+L+E K+T Y+ + + DG+ ++ +L + +++LRE++DK W + G A+VNAFY +N I FPAG+L+G FFN P Y+NYGA+G +T KF +K++CII Q NY +VG+KLNG+NTQGENIADNGG+ +AY A+ ++++ +G + LP L T +QLFW+S VWC K R E LK IL +H+P FRVNG LS EF+RD+ C S MNP KC
Sbjct: 318 ICLTKGCVTSASELIENMDPQMDPCEDFFEYACGNYLKTKNIPDEKSSISQFSDVSDVLQEKLRTLVESEDSSEDTPSSLMVKQLYKSCMNTERIAEQGLQPLKDILREMGGWPAVEGFAWDRNRFDWVQNVYINRRLGYSVDLLFDFSITTNIKNSSWRIIDIDQPSLGMPSREYLLRGLNDSDVRAYLDYQVSLAALLGANRTAAELELTESLLFEIQLANFSLPKEQRRNATKLYNKMTVAELQGRVPNIP--WLSYINTILSPFTTVTEREEVIVNVPSYIENLGKLLLRTDKRVIANYMMWRLSAASISYLSEDARDLQLVYSKKITGTGKRKPRWKECMGAVS------GSLSYAVGKLYAEKFFKKDAKAAADEMVSYIRAEFDKILRTVDWMDDETRIRAINKSLAITPHIAYPEELLDETKLTELYKDLQISDGELLSNMRNLTIFGTDYSFKRLREKVDKNDWKKHGAAAVVNAFYSPLENSIQFPAGILQGTFFNADRPKYLNYGAIGFVIGHEITHGFDDMGRQFDYKGDLKEWWKPETRAKFIDKSKCIIYQYGNYTEPEVGLKLNGVNTQGENIADNGGIKEAYYAYNQYVSDHGEENPLPALGLTAKQLFWLSAANVWCGKYRPETLKLRILTGAHSPARFRVNGPLSNLPEFSRDWNCKSSSKMNPSHKC 995 HSP 2 Score: 425.246 bits (1092), Expect = 1.432e-125 Identity = 209/463 (45.14%), Postives = 292/463 (63.07%), Query Frame = 0
Query: 894 GYSMDMMVDLYIYGDAKNSTWRTIHLDQPVLGL-SREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTHN-ITEDERVLIGNPEFFKKLSDVMTKASPRTLANYIGWRFVKSIVGITTRDARDAALEYAKVTSGTNELPARWETCVKATGFGAHSRNSFLFGTGSLYAKHYFPKEAKLGLKGLIRNVRASFKEILDDIDWMDDGTKGKAYEKLAAMDQMIGFPDEILEEDKVTSMYKGVQIQEGQHFQNILSLRRRSRQFQYERLREEVDKKAWHERATVAMVNALYSPTKNMILFPAGILGGFFFNTDAPPYHNYGAIGVVIGHEITHGFDDQGRRNDQNGDLADWWDEDTAQKFEDKAQCIINQANNFESAQVQLKLNGINTQGENIADNGGISEAYRAY 1353
GYS+D++ D I + KNS+WR I +DQP LG+ SREYL+ GL D V+AY Y + L+GA+R A+E EL + LLFE++LA S PKE+RR+ KLYN + + + +P P W S+++ ++ +TE E V++ P + + L ++ + R +ANY+ WR + + + DARD L Y+K +GT + RW+ C+ A S + G LYA+ +F K+AK ++ +RA F +IL +DWMDD T+ +A K A+ I +P+E+L+E K+T +YK +QI +G+ N+ +L + ++RLRE+VDK W + A+VNA YSP +N I FPAGIL G FFN D P Y NYGAIG VIGHEITHGFDD GR+ D GDL +WW +T KF DK++CII Q N+ +V LKLNG+NTQGENIADNGGI EAY AY
Sbjct: 456 GYSVDLLFDFSITTNIKNSSWRIIDIDQPSLGMPSREYLLRGLNDSDVRAYLDYQVSLAALLGANRTAAELELTESLLFEIQLANFSLPKEQRRNATKLYNKMTVAELQGRVPNIP--WLSYINTILSPFTTVTEREEVIVNVPSYIENLGKLLLRTDKRVIANYMMWRLSAASISYLSEDARDLQLVYSKKITGTGKRKPRWKECMGAVS------GSLSYAVGKLYAEKFFKKDAKAAADEMVSYIRAEFDKILRTVDWMDDETRIRAINKSLAITPHIAYPEELLDETKLTELYKDLQISDGELLSNMRNLTIFGTDYSFKRLREKVDKNDWKKHGAAAVVNAFYSPLENSIQFPAGILQGTFFNADRPKYLNYGAIGFVIGHEITHGFDDMGRQFDYKGDLKEWWKPETRAKFIDKSKCIIYQYGNYTEPEVGLKLNGVNTQGENIADNGGIKEAYYAY 910 HSP 3 Score: 224.557 bits (571), Expect = 2.980e-56 Identity = 108/283 (38.16%), Postives = 175/283 (61.84%), Query Frame = 0
Query: 611 GSPMNPEEKCRSMNRSMDPCENFYEFTCGRLVENTVIPEDKSSWSEIAILNKDLQQKGKFLLEKEGSQDDWEIFKDMRKFYRSCLNEDEMDKIAYEPLTEVLDTLGGWPVLEGKNWDRTDYKWYENIYYLNKEGYSMDMVVDLHIYGDAKNSTWRTIHLDQPMLGL-SREYLIEGLEDQHVKAYFKYMQDITELMGADRNASEAELKDVLLFEMELAQISAPKEERRDRNKLYNPTLLKDFES-LPGHPPSWTSFVDNLVYTH-NITEDERVLIGNPEFFKKL 890
G + E +M+ MDPCE+F+E+ CG ++ IP++KSS S+ + ++ LQ+K + L+E E S +D +++ Y+SC+N + + + +PL ++L +GGWP +EG WDR + W +N+Y + GYS+D++ D I + KNS+WR I +DQP LG+ SREYL+ GL D V+AY Y + L+GA+R A+E EL + LLFE++LA S PKE+RR+ KLYN + + + +P P W S+++ ++ +TE E V++ P + + L
Sbjct: 323 GCVTSASELIENMDPQMDPCEDFFEYACGNYLKTKNIPDEKSSISQFSDVSDVLQEKLRTLVESEDSSEDTPSSLMVKQLYKSCMNTERIAEQGLQPLKDILREMGGWPAVEGFAWDRNRFDWVQNVYINRRLGYSVDLLFDFSITTNIKNSSWRIIDIDQPSLGMPSREYLLRGLNDSDVRAYLDYQVSLAALLGANRTAAELELTESLLFEIQLANFSLPKEQRRNATKLYNKMTVAELQGRVPNIP--WLSYINTILSPFTTVTEREEVIVNVPSYIENL 603 The following BLAST results are available for this feature:
BLAST of PREDICTED: neprilysin-2-like vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 21
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BLAST of PREDICTED: neprilysin-2-like vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of PREDICTED: neprilysin-2-like vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold243_size241480:195202..207118- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold243_size241480-processed-gene-1.14 ID=snap_masked-scaffold243_size241480-processed-gene-1.14|Name=PREDICTED: neprilysin-2-like|organism=Tigriopus kingsejongensis|type=gene|length=11917bp|location=Sequence derived from alignment at scaffold243_size241480:195202..207118- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'PREDICTED: neprilysin-2-like' has the following synonyms
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