BLAST of hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000004591 (supercontig:LSalAtl2s:LSalAtl2s23:256216:264493:1 gene:EMLSAG00000004591 transcript:EMLSAT00000004591 description:"maker-LSalAtl2s23-augustus-gene-2.34")

HSP 1 Score: 393.66 bits (1010), Expect = 7.139e-126
Identity = 222/531 (41.81%), Postives = 317/531 (59.70%), Query Frame = 0

Query:  128 TRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLF---TAEQPDSVDGGYLNCLNPESLTVVKKAKVEPS-LAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWG 654
            TRFPPEPNG LHIGHA AI +NF  A  +      C+LR+DDTNP KE+ ++  +IK  + WLGY+  + +  +SD F+  Y+ A  LI+K LAY+  Q  ++IR           PSP + R + E+L+LF+ M+ G F +G A LR  + +E   +   DPV YR+ +A HH+TGD+WCIYP YD+ H L D+ E ITHSLC+ EF+N R  Y W+  N      P Q E+ RLN+ Y V+SKRK  +L+E G  + WDDPR+ TL+ +RRRG+PPEA+  FV R+G+T      D ++LE  VRS+LN  A R M VL+P+KV +TN      +   VP  P++ + G+R L+ + E +IE  DF     K + RL PG +V LR A I+ +    +G +    EI  EF+  +  KS P+       IHWV  ++  +  +RLYDRLF     E P  V GG+L  +NP+SL V++ + ++ S L  +   ++ QFER G+F  D  D  + + VFNR V+L++  G
Sbjct:  405 TRFPPEPNGILHIGHAKAININFAYADVH---NGTCNLRYDDTNPEKEEEKFFVAIKDMVEWLGYK-PDKITHSSDNFDQLYEWAKVLIQKDLAYICHQKVEDIRGFNP------PPSPWKNRPIEESLQLFEDMKHGKFEEGEATLRLKLTLEEGKV---DPVAYRIKYAAHHRTGDKWCIYPTYDYTHCLCDSIEDITHSLCTKEFQNRRSSYYWLC-NALDIYCPVQWEYGRLNVSYGVISKRKIAKLIETGVCSDWDDPRLFTLTALRRRGFPPEAVNNFVARLGVTGAQMTVDPSMLEASVRSYLNVHAPRCMVVLEPLKVKITNGTSLPKKVNDVPLFPDDPSKGTRSLSLSSEIYIEISDFNEVPEKGYRRLCPGQSVGLRYAGIVLNFLKKIGPN----EIAAEFVHVSEVKSKPKAF-----IHWVDASDSSNITIRLYDRLFKHANPEDPSVVPGGFLTDVNPDSLRVLEDSVIDSSVLKGMKNYDKFQFERNGFFSVDP-DTNSYRYVFNRTVSLKEDSG 911          

BLAST of hypothetical protein vs. L. salmonis genes
Match: EMLSAG00000000806 (supercontig:LSalAtl2s:LSalAtl2s1136:171985:178909:-1 gene:EMLSAG00000000806 transcript:EMLSAT00000000806 description:"maker-LSalAtl2s1136-augustus-gene-1.66")

HSP 1 Score: 210.69 bits (535), Expect = 9.348e-58
Identity = 164/519 (31.60%), Postives = 255/519 (49.13%), Query Frame = 0

Query:  116 ADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIERED--FLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCAD 632
             DL   +    I RFPPE +GYLH+GHA A  +N      +  QG +  LRFDDTNP KE  E+ + I  D+  L  ++ ++    SD+FE   +    +I++  AYVD    + ++A+R       T S +R  TV +N+ L+K+M+ G        +RA I+M S N  MRDP +YR  +  H  TG ++ +YP YDFA P+ D+ EG+TH+L + E+ +    + W + N      P    ++RL++  TVMSKRK    VE G V GW+DPR  T+ G+ RRG   EA++ F+   G ++     ++  +    R  ++  A R   V +   V+V N      E V    +P+N   G + L  + +  I+ +D   L +  K  F       + L G  +IR +     A+G+VS I  E L D   K   + ++  T +  VS A  V  +   +D + +    +  D  + N +N +S   V     +     LA G+  Q +R GYF  D
Sbjct:  830 VDLPGAEMGKVIVRFPPEASGYLHVGHAKACLLNQHYRDHF--QG-KLILRFDDTNPAKEKEEFENVILEDLKLLKVKY-DYFSRTSDHFETMLNYCEKMIKEETAYVDDTDAETMKAER----ETRTXSKNRSNTVEKNMNLWKEMKAGTDKGISCCVRAKINMLSDNGCMRDPTIYRCKNEPHPSTGSKYKVYPTYDFACPIVDSIEGVTHALRTTEYMDRDEQFFWFI-NALGLRQPHIYAYSRLSLTNTVMSKRKLTWFVESGQVEGWNDPRFPTVRGVIRRGLTIEALKNFILAQGSSRSVVFMEWDKIWAFNRKIIDPIAPRYTTVDESYHVLV-NINGLKEEXVEFDKHPKNSDVGKKTLWRSSKVIIDGDDAEALKENEKATF-------INL-GNVMIRKIHK---ANGKVSSIDGEPLLD--DKDYKKTLKV-TWLANVSGAGFVPTQCVYFDHIISKSVLEKND-DFKNFVNRDSKFTVNMIG-DHEFKKLAVGDIIQVQRRGYFIVD 1322          

BLAST of hypothetical protein vs. SwissProt
Match: gi|46577241|sp|Q87YQ1.1|SYQ_PSESM (RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS)

HSP 1 Score: 651.744 bits (1680), Expect = 0.000e+0
Identity = 306/544 (56.25%), Postives = 395/544 (72.61%), Query Frame = 0

Query:  112 PIISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGG--YLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSW 653
            PI+ ADL SGKH+  ITRFPPEPNGYLHIGHA +I VNFGLA+E+   G   HLRFDDTNP KED EY+D+I  D+ WLG+EW   + +AS YF+  +D AV LI+ G AYVD  TP++ R  RG +T  G  SP RER+V EN++LF +M+ G F DG  VLRA IDM S NMN+RDP+LYR+ HAHHH+TGD+WCIYP+YDF H   DA EGITHS+C+LEFE+HRPLY+W ++N PV   PRQ EF+RLN+ YT+ SKRK  QLV+E HVNGWDDPRMSTLSG RRRGY P++IR F E +G  + + + DF +LEF +R  L+ +A R M VL P+KVV+TNYPE   E + +P +P+ +  G R+L F+RE +I+R+D++ + PK + RL+P G VRLRG+Y+IR  + +  ADG + E+ C + PDT+GK+ PEG + +  +HWV  AE V+ EVRLYDRLF +  P+  + G  +L+ +NP+SL V+   + EPSL    P +R QFER GYFCAD +D    QPVFNR VTLRDSW
Sbjct:   30 PIVQADLDSGKHSKIITRFPPEPNGYLHIGHAKSICVNFGLAKEF---GGDTHLRFDDTNPAKEDQEYIDAIMNDVKWLGFEWAGEVRYASQYFDQLHDWAVELIKAGKAYVDDLTPEQAREYRGTLTEPGKNSPFRERSVEENIDLFARMKAGEFEDGARVLRAKIDMASPNMNLRDPILYRIRHAHHHQTGDKWCIYPIYDFTHGQSDAIEGITHSICTLEFESHRPLYDWFLDNLPVPCKPRQYEFSRLNLNYTITSKRKLKQLVDEKHVNGWDDPRMSTLSGFRRRGYTPKSIRNFCEMIGTNRSDGVVDFGMLEFSIRDDLDHSAPRAMCVLRPLKVVITNYPEGQVEKLELPRHPKEDL-GMRELPFSREIYIDRDDYMEEPPKGYKRLEPNGEVRLRGSYVIRADEAIKDADGNIVELRCSYDPDTLGKN-PEGRKVKGVVHWVPAAESVECEVRLYDRLFRSANPEKAEDGASFLDNINPDSLQVLTGCRAEPSLGHAQPEDRFQFEREGYFCADIKDSKPGQPVFNRTVTLRDSW 568          

BLAST of hypothetical protein vs. SwissProt
Match: gi|46577243|sp|Q88IU5.1|SYQ_PSEPK (RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS)

HSP 1 Score: 649.432 bits (1674), Expect = 0.000e+0
Identity = 306/546 (56.04%), Postives = 396/546 (72.53%), Query Frame = 0

Query:  112 PIISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVD--GGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWGA 655
            PII ADL SGKH++ +TRFPPEPNGYLHIGHA +I VNFGLA+E+   G  CHLRFDDTNP KED EY+D+I+ D+ WLG+EWG  + +ASDYF+  +D AV LI++G AYV   TP++ +  RGN+   G  SP RER+V ENL+LF +M+ G F DG  VLRA IDM S NMN+RDP+LYR+ HAHHH+TGD+WCIYP YDF H   DA EGITHS+C+LEFE HRPLY+W ++N PV + PRQ EF+RLN+ YT+ SKRK  QLV+E HV+ WDDPRMSTLSG RRRGY P +IR F E +G  + + + D ++LEF +R  L++ A R M VL P+KVV+TNYPE   E + +P +P+ +  G R L F+RE +I+R+DF+ + PK + RL+P G VRLRG+Y+IR  + +  ADG + E+ C + PDT+GK+ PEG + +  IHWV     V+ EVRLYDRLF +  P+  +  G +L+ +NP SL V+   + EPSLA   P +R QFER GYFCAD +D    +PVFNR VTLRDSWG+
Sbjct:   27 PIIQADLDSGKHSSIVTRFPPEPNGYLHIGHAKSICVNFGLAKEF---GGVCHLRFDDTNPAKEDQEYIDAIQSDVKWLGFEWGGDVRYASDYFDQLHDWAVELIKRGKAYVCDLTPEQAKEYRGNLKEPGKNSPFRERSVEENLDLFARMKAGEFKDGERVLRAKIDMASPNMNLRDPILYRIRHAHHHQTGDKWCIYPNYDFTHGQSDAIEGITHSICTLEFEGHRPLYDWFLDNLPVPAHPRQYEFSRLNLNYTITSKRKLKQLVDEKHVDAWDDPRMSTLSGFRRRGYTPASIRNFCEMIGTNRSDGVVDMSMLEFSIRDDLDRTAPRAMCVLRPLKVVITNYPEGQVEQLELPRHPKEDM-GVRVLPFSRELYIDRDDFMEEPPKGYKRLEPAGEVRLRGSYVIRADEAIKDADGNIVELRCSYDPDTLGKN-PEGRKVKGVIHWVPAEGSVECEVRLYDRLFRSPNPEKTEEGGSFLDNINPASLQVLSGCRAEPSLAQAQPEDRFQFEREGYFCADLKDSQPGRPVFNRTVTLRDSWGS 567          

BLAST of hypothetical protein vs. SwissProt
Match: gi|229558677|sp|A4VL72.1|SYQ_PSEU5 (RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS)

HSP 1 Score: 644.04 bits (1660), Expect = 0.000e+0
Identity = 311/545 (57.06%), Postives = 387/545 (71.01%), Query Frame = 0

Query:  112 PIISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQP--DSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWG 654
            PI+ ADL SGKHA  ITRFPPEPNGYLHIGHA +I +NFGLA+E+   G  C+LRFDDTNP KED EY+D+IK D+ WLG++W     +ASDYF+  +D AV LI+ G AYV   TP++ R  RG++T  GT SP RER+V ENL+LF +M+ G FPDG   LRA IDM S NMN+RDP+LYR+ HAHHH+TGD+WCIYP YDF H   DA EGITHS+C+LEFE+HRPLY W + N PV + PRQ EFARLN+ YT+ SKRK  QLV+E HV+GWDDPRMSTLSG RRRGY P +IR F + +G+ +   + D  +LEF +R  L+  A R M VL P+KVV+TNYP+D  E + +P +P+ +  G R L F+RE +I+  DF    P  F RL PGG VRLRG+Y+IR  + V    G + E+ C +  +T+GK+ PEG + +  IHWV  AE V+ EVRLYDRLF +  P  D   G +L+ +NPESL V+K  + EPSLA  AP ER QFER GYFCAD +D     PVFNR VTLRDSWG
Sbjct:   15 PIVQADLDSGKHAKIITRFPPEPNGYLHIGHAKSICLNFGLAKEF---GGECNLRFDDTNPAKEDQEYIDAIKSDVQWLGFQWAGEERYASDYFDQLHDWAVHLIKAGKAYVCDLTPEQAREYRGSLTEPGTNSPFRERSVEENLDLFARMKAGEFPDGARALRAKIDMASPNMNLRDPILYRIRHAHHHQTGDKWCIYPSYDFTHGQSDAIEGITHSICTLEFEDHRPLYEWFLANLPVPAQPRQYEFARLNLNYTITSKRKLKQLVDEKHVSGWDDPRMSTLSGFRRRGYTPASIRNFCDMIGVNRAGGVVDIGMLEFAIREDLDANAARAMCVLKPLKVVITNYPQDQVENLELPRHPKQDM-GVRVLPFSREIYIDAGDFEEVPPAGFKRLVPGGEVRLRGSYVIRADEAVKDDQGNIVELRCSYDENTLGKN-PEGRKVKGVIHWVPAAESVECEVRLYDRLFRSANPEKDEEGGSFLDNINPESLVVLKGCRAEPSLANAAPEERFQFEREGYFCADMKDSKPGAPVFNRTVTLRDSWG 554          

BLAST of hypothetical protein vs. SwissProt
Match: gi|229558676|sp|A4XVG2.1|SYQ_PSEMY (RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS)

HSP 1 Score: 630.943 bits (1626), Expect = 0.000e+0
Identity = 303/545 (55.60%), Postives = 386/545 (70.83%), Query Frame = 0

Query:  112 PIISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVD--GGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWG 654
            PI+ ADL SGKHA  +TRFPPEPNGYLHIGHA +I +NFGLA E+   G +C+LRFDDTNP KED EY+D+IK D+ WLG++W     +ASDYF+  +  AV LI+ G A+V     +E+R  RG++T  G  SP R+R+V ENL+LF +M+ G FPDG   LRA IDM S N+N+RDP+LYR+ HAHHH+TGD+WCIYP YDF H   DA EGITHS+C+LEFE+HRPLY W +EN PV + PRQ EFARLN+ YT+ SKRK  QLV+EGHV GWDDPRMSTLSG RRRGY P +IRAF + +G+ +   + D  +LEF +R  L+  A R M VL P+KVV+TNYPE   E + +P +P+ +  G R L F+RE +I+  DF    P  F RL PGG VRLRG+Y+IR  + +  A G + E+ C +  +T+GK+ PEG + +  IHWV  AE V+ EVRLYDRLF +  P+  +  G +L+ +NP+SL V+   + EPSLA  AP ER QFER GYF AD +D    +PVFNR VTLRDSWG
Sbjct:   15 PIVQADLDSGKHAKIVTRFPPEPNGYLHIGHAKSICLNFGLAEEF---GGQCNLRFDDTNPAKEDQEYIDAIKADVEWLGFKWAGEERYASDYFDQLHAWAVELIKAGKAFVCDLNAEEMRTYRGSLTEPGKNSPFRDRSVEENLDLFARMKAGEFPDGARSLRAKIDMASPNINLRDPILYRIRHAHHHQTGDKWCIYPSYDFTHGQSDAIEGITHSICTLEFEDHRPLYEWFLENLPVPAQPRQYEFARLNLNYTITSKRKLKQLVDEGHVQGWDDPRMSTLSGYRRRGYTPASIRAFCDMIGVNRAGGVVDIGMLEFAIREDLDANAARAMCVLKPLKVVITNYPEGKVENLELPRHPKQDM-GVRVLPFSREIYIDASDFEETPPDGFKRLIPGGEVRLRGSYVIRADEAIKDAAGNIVELRCSYDENTLGKN-PEGRKVKGVIHWVPAAESVECEVRLYDRLFRSANPEKSEEGGSFLDNINPDSLVVLTGCRAEPSLAQAAPEERFQFEREGYFVADLKDSQPGKPVFNRTVTLRDSWG 554          

BLAST of hypothetical protein vs. SwissProt
Match: gi|46577250|sp|Q89KR6.1|SYQ_BRADU (RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS)

HSP 1 Score: 629.402 bits (1622), Expect = 0.000e+0
Identity = 295/544 (54.23%), Postives = 392/544 (72.06%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWGAK 656
            II ADL   K+   +TRFPPEPNGYLHIGHA +I +NFG+A+E+     RCHLRFDDTNPVKE+ EY+DSI+ D+ WLG++WG++L+FASDYF+  Y+ A  LI  GLAYVD QT +EIR  RG +T  G  SP R+R+V ENL+LF++M+ G FP+G  VLRA IDM + N+N+RDPVLYR+LHAHH +TG+ WCIYP YD+AH   DA EGITHS+C+LEFE+HRPLY+W +E  PV S P Q EFARLN+ YT++SKR   QLV EGHV GWDDPRM T++GMRRRG PP A+R FV+R+G+ K NS+ D  +LEF +R  LN+ A R+MAVL P+KVV+ NYPE  +E +   N+P++ + G+RK+TF RE +IE++DF+ + PKKFFRL PG  VRLR AY ++    +    GEV E+ C + P T G +AP+G + +  +HW+S A    AE+R+Y++LF    PD+    +   LNP+SL ++  A+VE S+A     E  QFER GYF  D +D T  +PVF+R + LRD++  +
Sbjct:   17 IIQADLDQAKYREIVTRFPPEPNGYLHIGHAKSIALNFGIAQEFP---GRCHLRFDDTNPVKEEQEYIDSIQADVHWLGFDWGKNLFFASDYFDRLYEWAETLIRDGLAYVDDQTQEEIRTSRGTLTEPGKNSPFRDRSVDENLDLFRRMKAGEFPNGARVLRAKIDMAAGNINLRDPVLYRILHAHHPRTGNAWCIYPSYDYAHGQSDAIEGITHSICTLEFEDHRPLYDWFIEKLPVPSKPHQYEFARLNLTYTLLSKRVLTQLVREGHVAGWDDPRMPTMAGMRRRGVPPAALREFVKRIGVAKANSVVDVGMLEFCIREELNRTAQRRMAVLKPLKVVIENYPEGQTEELEAINHPDDPSAGTRKITFGRELYIEQDDFMENPPKKFFRLSPGNEVRLRYAYFVKCTGVIKNESGEVVELRCTYDPATRGGNAPDGRKVKATMHWLSAAASKPAEIRIYNQLFANPSPDA--SNFAADLNPQSLEILSNARVEASVAESNSTEPMQFERQGYFVRD-KDSTPGKPVFSRTIGLRDTFAKE 554          

BLAST of hypothetical protein vs. SwissProt
Match: gi|259511632|sp|C1DHE5.1|SYQ_AZOVD (RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS)

HSP 1 Score: 626.706 bits (1615), Expect = 0.000e+0
Identity = 301/545 (55.23%), Postives = 380/545 (69.72%), Query Frame = 0

Query:  112 PIISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVD--GGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWG 654
            PI+ ADL SGKHA  +TRFPPEPNGYLHIGHA +I +NFGLA+E+   G  C+LRFDDTNP KED EY+D+IK D+ WLG++W     +AS YF+  YD A+ LIE G AYV   +PDE R  RG++T  G  SP+RER+V ENL+LF +MR G FPDG   LRA IDM + NMN+RDP+LYR+ HAHHH+TGD+WCIYP YDF H   DA EGITHS+C+LEFE+HRPLY W ++N PV   PRQ EFARLN+ YT+ SKRK  QLV+EGHV GWDDPRMSTLSG RRRGY P +IRAF + +G+ +   + D  +LEF +R  L+  A R M VL P+KVV+TNYP+   E++ +P +P+ +  G R L F+RE +I+  DF    P  + RL PGG VRLRG+Y+IR  + +    G + E+ C +  +T+GK+ PEG + +  IHWV     +  EVRLYDRLF    P+  +  G +L+ +N  SL V+   + EPSLA   P ER QFER GYFCAD +D     PVFNR VTLRDSWG
Sbjct:   15 PIVQADLESGKHAKIVTRFPPEPNGYLHIGHAKSICLNFGLAQEF---GGECNLRFDDTNPAKEDQEYIDAIKSDVRWLGFQWAGEERYASSYFDQLYDWAIHLIEAGKAYVCDLSPDEAREYRGSLTEPGRNSPYRERSVEENLDLFARMRAGEFPDGARALRAKIDMAAPNMNLRDPILYRIRHAHHHQTGDKWCIYPSYDFTHGQSDAIEGITHSICTLEFEDHRPLYEWFLDNLPVPCRPRQYEFARLNLNYTITSKRKLKQLVDEGHVGGWDDPRMSTLSGYRRRGYTPASIRAFCDMIGVNRAGGVVDIGMLEFAIREDLDANAARAMCVLKPLKVVITNYPQGRVESLELPRHPKQDM-GVRVLPFSREIYIDAGDFEETPPAGYKRLIPGGEVRLRGSYVIRADEAIKDEAGNIVELRCSYDENTLGKN-PEGRKVKGVIHWVPLEGSIKCEVRLYDRLFKVANPEKSEEGGSFLDNINSGSLVVLTGCRAEPSLANARPEERFQFEREGYFCADLKDSRPGAPVFNRTVTLRDSWG 554          

BLAST of hypothetical protein vs. SwissProt
Match: gi|22096205|sp|Q9I2U8.1|SYQ_PSEAE (RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS)

HSP 1 Score: 621.698 bits (1602), Expect = 0.000e+0
Identity = 299/544 (54.96%), Postives = 384/544 (70.59%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVD--GGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWG 654
            I+ ADL +GKHA  +TRFPPEPNGYLHIGHA +I +NFGLA+E+A     CHLRFDDTNP KED EY+D+I+ DI WLG++W   + +AS+YF+  +  AV LI+ G A+V    P+E+R  RG +T  G  SP+R+R+V ENL+LF +M+ G FPDG   LRA IDM S NMN+RDP+LYR+ HAHHH+TGD+WCIYP YDF H   DA EGITHS+C+LEFE+HRPLY W + N PV + PRQ EF+RLN+ YTV SKRK  QLV+EGHV+GWDDPRMSTLSG RRRGY PE+IR F E +G+ + + + D  +LEF +R  L+  A R M VL P+KVV+TNYPE   E + +P +P+ +  G R L F RE +I+  DF    P  + RL PGG VRLRG+Y+IR  + +  ADG + E+ C + PDT+GK+ PEG + +  IHWV     V+ EVRLYDRLF +  P+  +  G +L+ +N +SL V+   + EPSL    P +R QFER GYF AD +D    +PVFNR VTLRDSWG
Sbjct:   16 IVQADLDAGKHAKIVTRFPPEPNGYLHIGHAKSICLNFGLAQEFA---GDCHLRFDDTNPAKEDQEYIDAIEADIKWLGFQWSGEVCYASNYFDQLHAWAVELIKAGKAFVCDLGPEEMREYRGTLTEPGRNSPYRDRSVEENLDLFARMKAGEFPDGARSLRAKIDMGSPNMNLRDPILYRIRHAHHHQTGDKWCIYPSYDFTHGQSDAIEGITHSICTLEFEDHRPLYEWFLANLPVPAQPRQYEFSRLNLNYTVTSKRKLKQLVDEGHVSGWDDPRMSTLSGYRRRGYTPESIRNFCEMIGVNRASGVVDIGMLEFSIRDHLDATAPRAMCVLKPLKVVITNYPEGQVENLELPRHPKEDM-GVRVLPFGRELFIDAGDFEEVPPAGYKRLIPGGEVRLRGSYVIRADEAIKDADGNIVELRCSYDPDTLGKN-PEGRKVKGVIHWVPAEGSVECEVRLYDRLFRSANPEKAEEGGSFLDNINADSLQVLAGCRAEPSLGQANPEDRFQFEREGYFVADLKDSRPGKPVFNRTVTLRDSWG 554          

BLAST of hypothetical protein vs. SwissProt
Match: gi|122258853|sp|Q02KT5.1|SYQ_PSEAB (RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS >gi|226701369|sp|B7VB85.1|SYQ_PSEA8 RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS)

HSP 1 Score: 621.698 bits (1602), Expect = 0.000e+0
Identity = 298/544 (54.78%), Postives = 384/544 (70.59%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVD--GGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWG 654
            I+ ADL +GKHA  +TRFPPEPNGYLHIGHA +I +NFGLA+E+A     CHLRFDDTNP KED EY+D+I+ DI WLG++W   + +AS+YF+  +  A+ LI+ G A+V    P+E+R  RG +T  G  SP+R+R+V ENL+LF +M+ G FPDG   LRA IDM S NMN+RDP+LYR+ HAHHH+TGD+WCIYP YDF H   DA EGITHS+C+LEFE+HRPLY W + N PV + PRQ EF+RLN+ YTV SKRK  QLV+EGHV+GWDDPRMSTLSG RRRGY PE+IR F E +G+ + + + D  +LEF +R  L+  A R M VL P+KVV+TNYPE   E + +P +P+ +  G R L F RE +I+  DF    P  + RL PGG VRLRG+Y+IR  + +  ADG + E+ C + PDT+GK+ PEG + +  IHWV     V+ EVRLYDRLF +  P+  +  G +L+ +N +SL V+   + EPSL    P +R QFER GYF AD +D    +PVFNR VTLRDSWG
Sbjct:   16 IVQADLDAGKHAKIVTRFPPEPNGYLHIGHAKSICLNFGLAQEFA---GDCHLRFDDTNPAKEDQEYIDAIEADIKWLGFQWSGEVCYASNYFDQLHAWAIELIKAGKAFVCDLGPEEMREYRGTLTEPGRNSPYRDRSVEENLDLFARMKAGEFPDGARSLRAKIDMGSPNMNLRDPILYRIRHAHHHQTGDKWCIYPSYDFTHGQSDAIEGITHSICTLEFEDHRPLYEWFLANLPVPAQPRQYEFSRLNLNYTVTSKRKLKQLVDEGHVSGWDDPRMSTLSGYRRRGYTPESIRNFCEMIGVNRASGVVDIGMLEFSIRDHLDATAPRAMCVLKPLKVVITNYPEGQVENLELPRHPKEDM-GVRVLPFGRELFIDAGDFEEVPPAGYKRLIPGGEVRLRGSYVIRADEAIKDADGNIVELRCSYDPDTLGKN-PEGRKVKGVIHWVPAEGSVECEVRLYDRLFRSANPEKAEEGGSFLDNINADSLQVLTGCRAEPSLGQANPEDRFQFEREGYFVADLKDSRPGKPVFNRTVTLRDSWG 554          

BLAST of hypothetical protein vs. SwissProt
Match: gi|123759309|sp|Q3SRI8.1|SYQ_NITWN (RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS)

HSP 1 Score: 617.076 bits (1590), Expect = 0.000e+0
Identity = 294/542 (54.24%), Postives = 388/542 (71.59%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWG 654
            I++ADL SG+H + +TRFPPEPNGYLH+GHA +I +NFG+A ++   G RCHLRFDDTNP KE+ E++D+I+RD+ WLG++WGEHLY+ASDYFE  Y  A  LI +G AYVD QT +EIR  RG +T  G  SP R+R   ENL+LF +MR G FP+G  VLRA IDM + N+N+RDPVLYR+LHA H +TG+ W IYP YDFAH   D+ E ITHS+C+LEFE+HRPLY+W ++N PV S PRQ EFARLN+ +T++SKR   +LV  GHV GWDDPRM TL+G++RRG P EA+R FV+R+G+ K NS  D  +L+F +R  LN++A R+MAVL P+KVV+ NYPE  SE +   N+P++ + G+R++ F RE +IE+EDF+ + PKKFFRL PG  VRLR AY I   + V    G+V E+ C + P T G +AP+G + +  +HWV+ A+ V AEVRLY  LF  EQPD+ +  + + LNP+S+ ++    VEP+LA    G   QFER GYF  D  D    + VFNR V LRD+W 
Sbjct:   17 IVAADLASGRHKSIVTRFPPEPNGYLHLGHAKSICLNFGIAEDF---GGRCHLRFDDTNPAKEEQEFIDAIQRDVRWLGFDWGEHLYYASDYFEQLYAWAQHLIREGKAYVDDQTQEEIRLARGTLTEPGRNSPFRDRPADENLDLFARMRAGEFPNGARVLRAKIDMAAGNINLRDPVLYRILHATHPRTGNVWSIYPSYDFAHGQSDSIEHITHSVCTLEFEDHRPLYDWFLDNLPVPSRPRQYEFARLNVTHTLLSKRVLTELVRGGHVAGWDDPRMPTLAGLQRRGVPAEALREFVKRIGVAKANSTVDVGMLDFAIREALNRSASRRMAVLRPLKVVIENYPEGQSEELEAVNHPDDPSQGTRRIRFGREIYIEQEDFMENPPKKFFRLSPGREVRLRYAYFITCREVVKNPAGDVLELRCTYDPATRGGNAPDGRKVKATMHWVNTADAVPAEVRLYGHLFETEQPDAAN--FASELNPQSVQILSGCMVEPALARDDAGAAVQFERQGYFYRD-RDSAPGRLVFNRTVGLRDTWA 552          

BLAST of hypothetical protein vs. SwissProt
Match: gi|22096197|sp|Q8Y199.1|SYQ_RALSO (RecName: Full=Glutamine--tRNA ligase; AltName: Full=Glutaminyl-tRNA synthetase; Short=GlnRS)

HSP 1 Score: 614.379 bits (1583), Expect = 0.000e+0
Identity = 313/563 (55.60%), Postives = 383/563 (68.03%), Query Frame = 0

Query:  113 IISADLTSGK---------HAAP--ITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEW----GEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVAST-----PRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDT-IGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWG 654
            II  DL  G          HA P  ITRFPPEPNGYLHIGHA +I VNFGLA+EY   G RCHLRFDDTNPVKEDTEYVDSI   + WLGY W    GEHLY+ASDYFE  Y  A  LI++G AY+D+Q+ ++I A RG+ T  GTPSP R+R+V ENL LF+ MR G + DG  VLRA IDM + N+ MRDPVLYR+ HAHHH+TGD WCIYPMYDF H + DA E ITHSLC+LEFEN+RPLY+WV+++   A       P Q EFARL++ Y + SKRK +QLV E  V+GWDDPRM TL G+RRRGY PE+I+ F ERVG++K +S  D ++LE  VR  L+  A R +AVLDPVK+++ N P D +E    P +P+    G R+   TRE WIEREDF    PK +FRL PG  VRLR  Y+I        ADG ++ +H   +PDT  G    + V+ +  IHWVS A  ++AEVRLYDRLFT  QPDS D  +L+ LNP++  VV  A +EP+LA   P +R QFER GYF AD  D    +PVFNR+V L+DSWG
Sbjct:   21 IIDTDLEQGTYAGRQDTAGHALPPIITRFPPEPNGYLHIGHAKSIWVNFGLAKEY---GGRCHLRFDDTNPVKEDTEYVDSIIDAVHWLGYSWQNGTGEHLYYASDYFEQLYGFAEVLIQRGAAYIDSQSAEQIAANRGDFTRPGTPSPFRDRSVEENLALFRDMRAGKYQDGQHVLRARIDMAAPNIVMRDPVLYRIRHAHHHRTGDAWCIYPMYDFTHCISDALENITHSLCTLEFENNRPLYDWVLDHLRDAGALPAPLPHQYEFARLHLTYAITSKRKLLQLVNEKRVDGWDDPRMPTLVGIRRRGYTPESIQLFCERVGVSKADSWIDMSILEAAVRDDLDARAPRSVAVLDPVKLILDNVPADFNEPCSAPVHPKQPELGRREFPLTRELWIEREDFTETPPKGYFRLFPGNKVRLRYGYVIECTGCDKDADGNITAVHANIIPDTKSGTPGADSVKVKGNIHWVSAAHALEAEVRLYDRLFTDPQPDSGDKNFLDALNPDAKRVV-TAYLEPTLATAKPEDRFQFERHGYFVADRIDSQPGKPVFNRVVGLKDSWG 579          

BLAST of hypothetical protein vs. nr
Match: gi|1200465827|gb|OUV81182.1| (glutamine--tRNA ligase [Verrucomicrobiaceae bacterium TMED137])

HSP 1 Score: 970.304 bits (2507), Expect = 0.000e+0
Identity = 449/544 (82.54%), Postives = 494/544 (90.81%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWGAK 656
            II+ D   GKH APITRFPPEPNGYLHIGHA AIGV FG+A EYAE GARCHLRFDDTNPVKED EYV+SIK DI WLGY+WGE+L+FASDYF++FYDCAV+LI+KGLAYVDTQT +EIR+QRGNV+ VGTPSPHR+R+V ENL+LF+KMREG F DGGAVLRANIDMESFNMNMRDPVLYRV+HAHHH TG +WCIYPMYDFAHPLEDAKE ITHSLCSLEFENHRPLYNWVVENCPV S PRQIEFARLNIGYT+MSKRKFIQLV+E HV GW+DPRMSTLSGMRRRGYPPEAIRAFV+RVGLTKQNSLSDFALLEFEVR++LNK ALRKMAVLDP+KV+VTNYPE  SE V VPNNPENE DG+R+LTFTREFWIER+DF+IDAPKK+FRLKPGGAVRLRGAYI + +DH +  DGEVSEIHC FLP+TIGKSAPEGVQCRTAIHWVSC E V AEVRLYDRLFT E PDS +GGYL+CLNP+SL+VV  A+VEPSLA   PGERCQFER+GYFC+DSEDHT  +PVFNRI TLRDSWG+K
Sbjct:   12 IIANDXXXGKHTAPITRFPPEPNGYLHIGHAKAIGVXFGIANEYAELGARCHLRFDDTNPVKEDAEYVESIKHDIEWLGYDWGEYLFFASDYFDFFYDCAVSLIKKGLAYVDTQTSEEIRSQRGNVSIVGTPSPHRDRSVDENLDLFRKMREGAFEDGGAVLRANIDMESFNMNMRDPVLYRVVHAHHHXTGXKWCIYPMYDFAHPLEDAKEQITHSLCSLEFENHRPLYNWVVENCPVPSKPRQIEFARLNIGYTIMSKRKFIQLVDEKHVVGWNDPRMSTLSGMRRRGYPPEAIRAFVDRVGLTKQNSLSDFALLEFEVRNYLNKVALRKMAVLDPIKVIVTNYPEGDSEKVTVPNNPENEXDGTRELTFTREFWIERDDFMIDAPKKYFRLKPGGAVRLRGAYIXQXIDHTLXDDGEVSEIHCXFLPNTIGKSAPEGVQCRTAIHWVSCQEAVSAEVRLYDRLFTEEAPDSAEGGYLSCLNPDSLSVVVNAQVEPSLATTTPGERCQFERIGYFCSDSEDHTLEKPVFNRISTLRDSWGSK 555          

BLAST of hypothetical protein vs. nr
Match: gi|517940193|ref|WP_019110401.1| (MULTISPECIES: glutamine--tRNA ligase/YqeY domain fusion protein [unclassified Verrucomicrobia (miscellaneous)])

HSP 1 Score: 968.378 bits (2502), Expect = 0.000e+0
Identity = 446/544 (81.99%), Postives = 496/544 (91.18%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWGAK 656
            II+ DL SGKH APITRFPPEPNGYLHIGHA AIG+NFG+A EYA  G+RCHLRFDDTNPVKEDTEYV+SIKRDI WLGY+WG+HL+FASDYF++FYDCAV+LI+KGLAYVDTQ P+EIR+QRGNV  VGTPSPHR+R+V ENL+LF++MREG F DGGAVLRANIDMESFNMNMRDPVLYRV+H  HH TGD+WCIYPMYDFAHPLEDAKE ITHSLCSLEFENHRPLYNWVVENCPV S PRQIEFARLNIGYT+MSKRKFIQLV+E HV GW+DPRMSTLSGMRRRGYPPEAIRAFV+RVGLTKQNSLSDFALLEFEVR+ LNK A RKMAVLDP+KV+VTNYPE   E V VPNNPEN+ DG+R+LTFTREFWIEREDF+IDAPKK+FRLKPGGAVRLRGAYII+H +H++  DGEV EIHC FLP+TIG+SAPEGVQCRTAIHWVSC E V+AEVRLYDRLFT E PDS +GGYL+CLNP+SL+VV  A+VEPSLAA+A GERCQFER+GYFC D+EDHT  +PVFNRI TLRDSWG+K
Sbjct:   12 IIANDLESGKHTAPITRFPPEPNGYLHIGHAKAIGLNFGIANEYAGSGSRCHLRFDDTNPVKEDTEYVESIKRDIEWLGYDWGDHLFFASDYFDFFYDCAVSLIKKGLAYVDTQKPEEIRSQRGNVNIVGTPSPHRDRSVDENLDLFRQMREGVFKDGGAVLRANIDMESFNMNMRDPVLYRVVHTQHHHTGDKWCIYPMYDFAHPLEDAKEHITHSLCSLEFENHRPLYNWVVENCPVPSKPRQIEFARLNIGYTIMSKRKFIQLVDEKHVGGWNDPRMSTLSGMRRRGYPPEAIRAFVDRVGLTKQNSLSDFALLEFEVRNHLNKVAFRKMAVLDPIKVIVTNYPEGDCEKVTVPNNPENKDDGTRELTFTREFWIEREDFMIDAPKKYFRLKPGGAVRLRGAYIIQHTEHLLDDDGEVLEIHCHFLPNTIGQSAPEGVQCRTAIHWVSCQEAVNAEVRLYDRLFTEEVPDSAEGGYLSCLNPDSLSVVTNAQVEPSLAAMAAGERCQFERIGYFCTDTEDHTLERPVFNRISTLRDSWGSK 555          

BLAST of hypothetical protein vs. nr
Match: gi|1200391747|gb|OUV09883.1| (glutamine--tRNA ligase [Verrucomicrobiaceae bacterium TMED86])

HSP 1 Score: 964.14 bits (2491), Expect = 0.000e+0
Identity = 450/545 (82.57%), Postives = 490/545 (89.91%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQPVFNRIVTLRDSWGAKN 657
            II++D+ SGKH APITRFPPEPNGYLHIGHA AIGVNFGLA+EY E GARCHLRFDDTNPVKEDTEYVD+IK+ IAWLGY+WGEHLYFASDYFE+FYDCAV+LIEK LAYVDTQTP+EIR QRGNVT  GTPSPHRER+V E+LELFKKMREGGF DGGAVLRANIDMES NMNMRDPVLYRV+HA HH TGD WC+YPMYDFAHPLEDAKEGITHSLCSLEFENHRPLY+WVV NCPV S PRQIEFARLNIGYTVMSKRKFIQLV+EGHVNGWD PRMSTLSGMRRRGYPPEAIRAFV+RVGLTKQNSLSDFALLEFEVR +LNK ALRKMAVLDPVKVVVTNYPE  SE V  PNNPENE DG R+L FTREFWIER+DF++DAPKKFFRL+P GAVRLRGAYII+H+ HV+G DGEV+EIH EF+PDTIG+ APEG++CRTAIHWVS  + VDAEVR YDRLF+ E PD  +GGYL CLNP SL V+  AKVEPSLAA++ G  CQFERLGYF AD EDHTA +PVFNRIVTL+DSWG K+
Sbjct:   12 IIASDIESGKHXAPITRFPPEPNGYLHIGHAKAIGVNFGLAKEYGEIGARCHLRFDDTNPVKEDTEYVDAIKKXIAWLGYDWGEHLYFASDYFEFFYDCAVSLIEKKLAYVDTQTPEEIRKQRGNVTEAGTPSPHRERSVEESLELFKKMREGGFEDGGAVLRANIDMESGNMNMRDPVLYRVVHATHHNTGDAWCMYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYDWVVNNCPVPSVPRQIEFARLNIGYTVMSKRKFIQLVDEGHVNGWDXPRMSTLSGMRRRGYPPEAIRAFVDRVGLTKQNSLSDFALLEFEVRDYLNKVALRKMAVLDPVKVVVTNYPEGQSEIVXAPNNPENEEDGLRELKFTREFWIERDDFMVDAPKKFFRLRPEGAVRLRGAYIIKHVGHVLGDDGEVNEIHVEFIPDTIGQPAPEGIKCRTAIHWVSVEDAVDAEVRQYDRLFSEELPDKAEGGYLECLNPNSLEVITGAKVEPSLAAMSAGSHCQFERLGYFSADLEDHTAEKPVFNRIVTLKDSWGKKH 556          

BLAST of hypothetical protein vs. nr
Match: gi|1353604271|ref|WP_105041816.1| (glutamine--tRNA ligase/YqeY domain fusion protein [Rubritalea profundi] >gi|1350981252|gb|PQJ27334.1| glutamine--tRNA ligase [Rubritalea profundi])

HSP 1 Score: 763.066 bits (1969), Expect = 0.000e+0
Identity = 355/545 (65.14%), Postives = 434/545 (79.63%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQP-VFNRIVTLRDSWGAK 656
            II+ DLT+GKH   ITRFPPEPNGYLHIGHA AI +NFG+A + A  GA CHLRFDDTNPVKED  YVD+IK+D+AWLG++WGE+LYFASDYFEYFYDCAV LI++G AYVD Q  +E+RA RGNV   GT SP+R+ +V ENL  F++M++G F  GGAVLRA IDM + NMNMRDP +YRVL+  HHKTGD W IYPMYDFAHPLEDAKE +THSLCSLEFE+HRPLY WVV++CP A+TPRQIEF+RLN+ YT+MSKRK +QLVEE  V+GWDDPR+ TLSGMRRRGY PEAI++F+++VG+TK N+++D ALLEFE+R+ LNK A R+MAVLDP+K+V+ N+  D+ E +   NNPE+   G+R +   +E +IER+DF+ D PKK+FRL PG AVRLRG YII+++ H   ADG V+E+H EF+P T+G+S PEGV+C+ AIHWVS A  VDAEVRLYDRLFT E PD  +GGY+ C NP+SL V   AK+EPSL   A G   QFER+GYFC+DSE H + Q  VFNR V LRDSW  K
Sbjct:   14 IIAEDLTNGKHQTTITRFPPEPNGYLHIGHAKAICINFGIAEDNAGSGAVCHLRFDDTNPVKEDAAYVDAIKKDVAWLGFDWGENLYFASDYFEYFYDCAVHLIKQGNAYVDLQNVEEMRAGRGNVKTPGTDSPYRDSSVEENLTRFEQMKKGEFEVGGAVLRAKIDMAARNMNMRDPAIYRVLNIDHHKTGDAWNIYPMYDFAHPLEDAKEKVTHSLCSLEFEDHRPLYEWVVDHCPTAATPRQIEFSRLNLTYTIMSKRKLLQLVEEEVVDGWDDPRLPTLSGMRRRGYTPEAIKSFIQKVGVTKFNAITDVALLEFELRAHLNKIAERRMAVLDPLKIVIDNWDGDSVEEMECKNNPEDAEAGTRLVPLGKEVFIERDDFMEDPPKKYFRLGPGRAVRLRGGYIIQYVSHENDADGNVTEVHVEFIPGTVGQSPPEGVKCKAAIHWVSAAHAVDAEVRLYDRLFTEENPDGFEGGYMACWNPDSLEVKTTAKLEPSLGVAAAGTTFQFERVGYFCSDSEQHISGQKAVFNRTVGLRDSWAKK 558          

BLAST of hypothetical protein vs. nr
Match: gi|1332714349|ref|WP_102725544.1| (glutamine--tRNA ligase/YqeY domain fusion protein [Akkermansia muciniphila] >gi|1329749006|gb|PNC04882.1| glutamine--tRNA ligase [Akkermansia muciniphila])

HSP 1 Score: 761.14 bits (1964), Expect = 0.000e+0
Identity = 354/545 (64.95%), Postives = 424/545 (77.80%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQ-PVFNRIVTLRDSWGAK 656
            +IS DL SGKH AP+TRFPPEPNGYLHIGHA +I +NFG+A+E+   GARCHLRFDDTNP KED EYVDSI+ DI WLG++WG++L+FAS+ F +F++CAVALI+KGLAYVD QT +EIRAQRGNV   G  SP+R R+V ENL  F+ M+ G  P+G A+LRA IDM S NMNMRDPVLYR++ A HH TG+ WCIYPMYDFAHPLEDA E ITHSLC+LEFENHRPLY+WV+ENCPV + PRQ EFARLN+ YTVMSKRK +QLV+EGHV GWDDPRM T+SGMRRRGY P AIR F + +G+TK N  +D ALLE+ VR  LN  A R+MAVL+P+KV +T  PEDA E   V NNPE   +GSR++  TRE WIER+DF++D PKK+FRL PG  VRLRG Y I   D+   ADG ++EI CE +P TIG + PEG+QCR AIHWVS  + VD E+R+YDRLFT E PD+ + G+L+ +NP SLTV+  AK+EPSL+A  P  RCQFERLGYF AD  DH   Q PVFNR V L+DSW  K
Sbjct:   15 MISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFP--GARCHLRFDDTNPSKEDQEYVDSIQEDIRWLGFDWGKNLFFASNMFGFFHECAVALIKKGLAYVDEQTVEEIRAQRGNVNVPGVESPYRNRSVEENLARFQAMKNGEIPEGKAILRAKIDMASSNMNMRDPVLYRIMFAEHHNTGNTWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRPLYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVTGWDDPRMPTVSGMRRRGYTPAAIRNFCQTIGITKFNGFTDVALLEYSVREDLNANAPRRMAVLNPLKVTITTLPEDAEEMAEVLNNPEKPEEGSRQIPLTREVWIERDDFMLDPPKKYFRLAPGRTVRLRGGYCITCTDYRQDADGNITEILCEHIPGTIGSNPPEGIQCRAAIHWVSTRDAVDGEIRIYDRLFTEENPDAAEEGFLSVMNPGSLTVITNAKLEPSLSAAEPEFRCQFERLGYFVADRRDHVPGQRPVFNRTVALKDSWAKK 557          

BLAST of hypothetical protein vs. nr
Match: gi|1332695897|ref|WP_102711914.1| (glutamine--tRNA ligase/YqeY domain fusion protein [Akkermansia muciniphila] >gi|1329764361|gb|PNC19751.1| glutamine--tRNA ligase [Akkermansia muciniphila])

HSP 1 Score: 760.755 bits (1963), Expect = 0.000e+0
Identity = 354/545 (64.95%), Postives = 423/545 (77.61%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAAQ-PVFNRIVTLRDSWGAK 656
            +IS DL SGKH AP+TRFPPEPNGYLHIGHA +I +NFG+A+E+   GARCHLRFDDTNP KED EYVDSI+ DI WLG++WG++L+FAS+ F +F++CAVALI+KGLAYVD QT +EIRAQRGNV   G  SP+R R+V ENL  F+ M+ G  P+G A+LRA IDM S NMNMRDPVLYR++ A HH TG+ WCIYPMYDFAHPLEDA E ITHSLC+LEFENHRPLY+WV+ENCPV + PRQ EFARLN+ YTVMSKRK +QLV+EGHV GWDDPRM T+SGMRRRGY P AIR F + +G+TK N  +D ALLE+ VR  LN  A R+MAVL+P+KV +T  PEDA E   V NNPE   +GSR++  TRE WIER+DF++D PKK+FRL PG  VRLRG Y I   D+   ADG ++EI CE +P TIG + PEG+QCR AIHWVS  + VD E+R+YDRLFT E PD+ + G+L+ +NP SLTV+  AK+EPSL A  P  RCQFERLGYF AD  DH   Q PVFNR V L+DSW  K
Sbjct:   15 MISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFP--GARCHLRFDDTNPSKEDQEYVDSIQEDIRWLGFDWGKNLFFASNMFGFFHECAVALIKKGLAYVDEQTVEEIRAQRGNVNVPGVESPYRNRSVEENLARFQAMKNGEIPEGKAILRAKIDMASSNMNMRDPVLYRIMFAEHHNTGNTWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRPLYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVTGWDDPRMPTVSGMRRRGYTPAAIRNFCQTIGITKFNGFTDVALLEYSVREDLNANAPRRMAVLNPLKVTITTLPEDAEEMAEVLNNPEKPEEGSRQIPLTREVWIERDDFMLDPPKKYFRLAPGRTVRLRGGYCITCTDYRQDADGNITEILCEHIPGTIGSNPPEGIQCRAAIHWVSTRDAVDGEIRIYDRLFTEENPDAAEEGFLSVMNPGSLTVITNAKLEPSLGAAEPEFRCQFERLGYFVADRRDHVPGQRPVFNRTVALKDSWAKK 557          

BLAST of hypothetical protein vs. nr
Match: gi|1332717280|ref|WP_102728445.1| (glutamine--tRNA ligase/YqeY domain fusion protein [Akkermansia muciniphila] >gi|1329771083|gb|PNC26413.1| glutamine--tRNA ligase [Akkermansia muciniphila] >gi|1329788741|gb|PNC43798.1| glutamine--tRNA ligase [Akkermansia muciniphila])

HSP 1 Score: 755.362 bits (1949), Expect = 0.000e+0
Identity = 351/545 (64.40%), Postives = 421/545 (77.25%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHT-AAQPVFNRIVTLRDSWGAK 656
            +IS DL SGKH AP+TRFPPEPNGYLHIGHA +I +NFG+A+E+   GARCHLRFDDTNP KED EYVDSI+ DI WLG++WG++L+FAS+ F++FY+CAVALI KGLAYVD QT +EIRAQRGNV   G  SP+R R+V ENL  F+ M+ G  P+G A+LRA IDM S NMNMRDPVLYR++ A HH TG  WCIYPMYDFAHPLEDA E ITHSLC+LEFENHRPLY+WV+ENCPV + PRQ EFARLN+ YTVMSKRK +QLV+EGHV+GWDDPRM T+SGMRRRGY P AIR F + +G+TK N  +D ALLE+ VR  LN  A R+MAVL+P+KV +T  PE + E   V NNPE   +G+R+L  TRE WIER+DF++D PKK+FRL PG  VRLRG Y I   D+   ADG ++EI CE +P TIG + PEG+QCR AIHWVS  + VD E+R+YDRLFT E PD+ + G+L+ +NP SLTV+  AK+EPSLA   P  RCQFERLGYF AD  DH    + VFNR V L+DSW  K
Sbjct:   15 MISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFP--GARCHLRFDDTNPSKEDQEYVDSIQEDIRWLGFDWGDNLFFASNMFDFFYECAVALINKGLAYVDEQTVEEIRAQRGNVNVPGQESPYRNRSVEENLARFQAMKNGEIPEGKAILRAKIDMASSNMNMRDPVLYRIMFAEHHNTGGTWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRPLYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVSGWDDPRMPTVSGMRRRGYTPAAIRNFCQTIGITKFNGFTDVALLEYSVREDLNANAPRRMAVLNPLKVTITTLPEGSEEMAEVLNNPEKPEEGTRRLPITREVWIERDDFMLDPPKKYFRLAPGRTVRLRGGYCITCTDYRQDADGNITEIFCEHIPGTIGSNPPEGIQCRAAIHWVSTRDAVDGEIRIYDRLFTEENPDAAEEGFLSVMNPSSLTVITNAKLEPSLAEAEPEFRCQFERLGYFVADRRDHVPGKRAVFNRTVALKDSWAKK 557          

BLAST of hypothetical protein vs. nr
Match: gi|1228037280|ref|WP_094137397.1| (glutamine--tRNA ligase/YqeY domain fusion protein [Akkermansia muciniphila])

HSP 1 Score: 755.362 bits (1949), Expect = 0.000e+0
Identity = 351/545 (64.40%), Postives = 421/545 (77.25%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAA-QPVFNRIVTLRDSWGAK 656
            +IS DL SGKH AP+TRFPPEPNGYLHIGHA +I +NFG+A+E+   GARCHLRFDDTNP KED EYVDSI+ DI WLG++WG++L+FAS+ F +FY+CAV+LI KGLAYVD QT +EIRAQRGNV   G  SP+R R+V ENLE F+ M+ G  P+G A+LRA IDM S NMNMRDPVLYR++ A HH TG  WCIYPMYDFAHPLEDA E ITHSLC+LEFENHRPLY+WV+ENCPV + PRQ EFARLN+ YTVMSKRK +QLV+EGHV+GWDDPRM T+SGMRRRGY P AIR F + +G+TK N  +D ALLE+ VR  LN  A R+MAVL+P+KV +T  PE+A E   V NNPE   +G R+L  TRE WIER+DF++D PKK+FRL PG  VRLRG Y I   D+   A+G ++EI CE +P TIG + PEG+QCR AIHWVS    VD E+R+YDRLFT E PD+ + G+L+ +NP SL+V+  AK+EPSLA   P  RCQFERLGYF AD  DH    +PVFNR V L+DSW  K
Sbjct:   15 MISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFP--GARCHLRFDDTNPSKEDQEYVDSIQEDIRWLGFDWGDNLFFASNMFGFFYECAVSLIRKGLAYVDEQTVEEIRAQRGNVNVPGQESPYRNRSVEENLERFQAMKNGEIPEGRAILRARIDMSSSNMNMRDPVLYRIMFAEHHNTGSSWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRPLYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVSGWDDPRMPTVSGMRRRGYTPAAIRNFCQTIGITKFNGFTDVALLEYSVREDLNANAPRRMAVLNPLKVTITTLPENAEEMAEVLNNPEKPEEGVRRLPITREVWIERDDFMLDPPKKYFRLAPGRTVRLRGGYCITCTDYKQDAEGNITEIFCEHIPGTIGSNPPEGIQCRAAIHWVSTKYAVDGEIRIYDRLFTEENPDAAEEGFLSVMNPSSLSVITNAKLEPSLAGAEPEFRCQFERLGYFVADRRDHVPGRRPVFNRTVALKDSWAKK 557          

BLAST of hypothetical protein vs. nr
Match: gi|1332774038|ref|WP_102758692.1| (glutamine--tRNA ligase/YqeY domain fusion protein [Akkermansia muciniphila] >gi|1125127130|gb|OLA88142.1| glutamine--tRNA ligase [Akkermansia sp. 54_46] >gi|1329808119|gb|PNC62897.1| glutamine--tRNA ligase [Akkermansia muciniphila])

HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0
Identity = 351/546 (64.29%), Postives = 420/546 (76.92%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAA-QPVFNRIVTLRDSWGAKN 657
            +IS DL SGKH AP+TRFPPEPNGYLHIGHA +I +NFG+A+E+   GARCHLRFDDTNP KED EYVDSI+ DI WLG++WG++L+FAS+ F++FY+CAV+LI KGLAYVD QT +EIRAQRGNV   G  SP+R R+V ENL  F+ M+ G  P+G A+LRA IDM S NMNMRDPVLYR++ A HH TG  WCIYPMYDFAHPLEDA E ITHSLC+LEFENHRPLY+WV+ENCPV + PRQ EFARLN+ YTVMSKRK +QLV+EGHV+GWDDPRM T+SGMRRRGY P AIR F   +G+TK N  +D ALLE+ VR  LN  A R+MAVL+P+KV +T  PE+A E   V NNPE   +G R+L  TRE WIER+DF++D PKK+FRL PG  VRLRG Y I   D+   A+G ++EI CE +P TIG + PEG+QCR AIHWVS    VD E+R+YDRLFT E PD+ + G+L+ +NP SLTV+  AK+EPSLA   P  RCQFERLGYF AD  DH    +PVFNR V L+DSW  K 
Sbjct:   15 MISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFP--GARCHLRFDDTNPSKEDQEYVDSIQEDIRWLGFDWGDNLFFASNMFDFFYECAVSLIRKGLAYVDEQTVEEIRAQRGNVNVPGQESPYRNRSVEENLARFQAMKNGEIPEGKAILRARIDMASSNMNMRDPVLYRIMFAEHHNTGSSWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRPLYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVSGWDDPRMPTVSGMRRRGYTPAAIRNFCHTIGITKFNGFTDVALLEYSVREDLNANAPRRMAVLNPLKVTITTLPENAEEMAEVLNNPEKPEEGVRRLPITREVWIERDDFMLDPPKKYFRLAPGRTVRLRGGYCITCTDYKQDAEGTITEIFCEHIPGTIGSNPPEGIQCRAAIHWVSTKYAVDGEIRIYDRLFTEENPDAAEEGFLSVMNPSSLTVITNAKLEPSLAGAEPEFRCQFERLGYFVADRRDHVPGRRPVFNRTVALKDSWAKKQ 558          

BLAST of hypothetical protein vs. nr
Match: gi|1332727867|ref|WP_102735465.1| (glutamine--tRNA ligase/YqeY domain fusion protein [Akkermansia muciniphila] >gi|1329802021|gb|PNC56878.1| glutamine--tRNA ligase [Akkermansia muciniphila])

HSP 1 Score: 754.592 bits (1947), Expect = 0.000e+0
Identity = 351/546 (64.29%), Postives = 420/546 (76.92%), Query Frame = 0

Query:  113 IISADLTSGKHAAPITRFPPEPNGYLHIGHANAIGVNFGLAREYAEQGARCHLRFDDTNPVKEDTEYVDSIKRDIAWLGYEWGEHLYFASDYFEYFYDCAVALIEKGLAYVDTQTPDEIRAQRGNVTAVGTPSPHRERTVAENLELFKKMREGGFPDGGAVLRANIDMESFNMNMRDPVLYRVLHAHHHKTGDEWCIYPMYDFAHPLEDAKEGITHSLCSLEFENHRPLYNWVVENCPVASTPRQIEFARLNIGYTVMSKRKFIQLVEEGHVNGWDDPRMSTLSGMRRRGYPPEAIRAFVERVGLTKQNSLSDFALLEFEVRSFLNKAALRKMAVLDPVKVVVTNYPEDASETVIVPNNPENEADGSRKLTFTREFWIEREDFLIDAPKKFFRLKPGGAVRLRGAYIIRHLDHVVGADGEVSEIHCEFLPDTIGKSAPEGVQCRTAIHWVSCAEGVDAEVRLYDRLFTAEQPDSVDGGYLNCLNPESLTVVKKAKVEPSLAALAPGERCQFERLGYFCADSEDHTAA-QPVFNRIVTLRDSWGAKN 657
            +IS DL SGKH AP+TRFPPEPNGYLHIGHA +I +NFG+A+E+   GARCHLRFDDTNP KED EYVDSI+ DI WLG++WG++L+FAS+ F++FY+CAV+LI KGLAYVD QT +EIRAQRGNV   G  SP+R R+V ENL  F+ M+ G  P+G A+LRA IDM S NMNMRDPVLYR++ A HH TG  WCIYPMYDFAHPLEDA E ITHSLC+LEFENHRPLY+WV+ENCPV + PRQ EFARLN+ YTVMSKRK +QLV+EGHV+GWDDPRM T+SGMRRRGY P AIR F   +G+TK N  +D ALLE+ VR  LN  A R+MAVL+P+KV +T  PE+A E   V NNPE   +G R+L  TRE WIER+DF++D PKK+FRL PG  VRLRG Y I   D+   A+G ++EI CE +P TIG + PEG+QCR AIHWVS    VD E+R+YDRLFT E PD+ + G+L+ +NP SLTV+  AK+EPSLA   P  RCQFERLGYF AD  DH    +PVFNR V L+DSW  K 
Sbjct:   15 MISDDLASGKHQAPVTRFPPEPNGYLHIGHAKSICLNFGIAQEFP--GARCHLRFDDTNPSKEDQEYVDSIQEDIRWLGFDWGDNLFFASNMFDFFYECAVSLIRKGLAYVDEQTVEEIRAQRGNVNVPGQESPYRNRSVEENLARFQAMKNGEIPEGKAILRARIDMASSNMNMRDPVLYRIMFAEHHNTGSSWCIYPMYDFAHPLEDAYEHITHSLCTLEFENHRPLYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQLVQEGHVSGWDDPRMPTVSGMRRRGYTPAAIRNFCHTIGITKFNGFTDAALLEYSVREDLNANAPRRMAVLNPLKVTITTLPENAEEMAEVLNNPEKPEEGVRRLPITREVWIERDDFMLDPPKKYFRLAPGRTVRLRGGYCITCTDYKQDAEGTITEIFCEHIPGTIGSNPPEGIQCRAAIHWVSTKYAVDGEIRIYDRLFTEENPDAAEEGFLSVMNPSSLTVITNAKLEPSLAGAEPEFRCQFERLGYFVADRRDHVPGRRPVFNRTVALKDSWAKKQ 558