long-chain fatty acid-- ligase, snap_masked-scaffold4073_size6756-processed-gene-0.2 (gene) Tigriopus kingsejongensis

Overview
Namelong-chain fatty acid-- ligase
Unique Namesnap_masked-scaffold4073_size6756-processed-gene-0.2
Typegene
OrganismTigriopus kingsejongensis (Tigriopus kingsejongensis)
Associated RNAi Experiments

Nothing found

Homology
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 251.906 bits (642), Expect = 5.109e-77
Identity = 169/515 (32.82%), Postives = 262/515 (50.87%), Query Frame = 0
Query:    2 GNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHP-VEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCV-VTALPLYHIFALTANLLTFTRLGVRTLLITNPR-DMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPL--LEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRD-ENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGY-KIPRYVQFVDELPMTNVGKILRRELR 509
            G   T+  V   +  F S L   +G  KGD M +++PN  ++ IA +G    GV +   NP Y P E+  QL +SGA+ ++ + LF   +++    +  ++ +++  + +     K  +  A+                  F+D    G+     +    H D+  L Y+ GTTG  KG  LTH N+V N+ Q       + I  E +  + +  LP +HIFA+T +L    R GV+  LIT P+ +   FI  L   + T    V  L + + ++P  K    +  K+  GG       + E++ +     L   E YG+TE SP   + P +   Y GS G  I +T   + D + G+ L  GE GELC+ GPQ+M+GY++   AT +++ Q+GW  TGD+A  ++ G  YIVDRLK+++ V G  V P+E+ED+   +  +  V  +GVPDER+GE  +   VR + SV EQ +IDF  E +  + K+   V FVD LP    GKILRREL+
Sbjct:   85 GRTYTYEMVHSLSQKFGSALLR-LGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQKIIVIGMEETPSDCKSFMEMAI------------------FDD----GSFFDQRETIDPHKDILALPYSSGTTGPPKGVALTHSNLVNNITQLSHPSIHNFIGDEVEQHISIAVLPFFHIFAMTCSLSAGLRFGVK--LITLPKFEPEMFINSLVQYKPTHMLVVPPLVSFMTSSPLIKPSLLSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQEGYGMTECSPVTHVQPKDGIIY-GSCGNLIPNTIGKIIDIDTGKVLGPGENGELCVSGPQIMQGYYKNKKATDKII-QNGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVVRKNRSVMEQGIIDFVTEKVAPHKKLGAGVMFVDTLPKNQTGKILRRELK 572          
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 144.821 bits (364), Expect = 6.186e-37
Identity = 95/346 (27.46%), Postives = 161/346 (46.53%), Query Frame = 0
Query:  167 LQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSE-NDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASI-NPMN-IEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELR 509
            +Q+T GTTG+ K   L+H  ++ N L     L+ + I  +     +   +PL+H F      L     G + +L ++  D    +  +   + T   G  T++  +L                                    K L   YG TET P + I  P +  E    ++G P+   +  + D+NG+ L +   GEL +R P +M GY++   ++ +V+  D +  +GD+  + EKG+  I+ RL DMV+  G N+YP EIED    H  I+ V ++GVPD+  GE +    +  ++   E DL + C+  +  +KIP+Y  FV + P T  GK+ + E++
Sbjct:    4 IQFTSGTTGLPKAVALSHFCVINNAL-----LTGNRIGFDLKYHXINLWVPLFHTFGCVTGTLISLCYGSKLVLPSDGFDPTACLNCIPAEKCTYLFGTPTMYIAVLEAQ---------------------------------KKLRNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEEL-CAWICLEEGAKEFDLKEHCKGKIAHFKIPKYPMFVKDFPKTLSGKVKKFEMK 310          

HSP 2 Score: 138.658 bits (348), Expect = 6.539e-35
Identity = 74/222 (33.33%), Postives = 123/222 (55.41%), Query Frame = 0
Query:  290 GGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASI-NPMN-IEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELR 509
            GG   +  A  +  + +  K L+  YG TET P + I  P +  E    ++G P+   +  + D+NG+ L +   GEL +R P +M GY++   ++ +V+  D +  +GD+  + EKG+  I+ RL DMV+  G N+YP EIEDV   H  I+ V ++GVPD   GE +    +  ++   E DL + C+  ++ +KIP+Y  FV + P T  GK+ + E++
Sbjct:  318 GGSNCLVEACKDFEKHLNMKSLMNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEEL-CAWICLEEGAKEFDLKEHCKGKISHFKIPKYPMFVKDFPKTLSGKVKKFEMK 538          

HSP 3 Score: 138.658 bits (348), Expect = 7.435e-35
Identity = 80/258 (31.01%), Postives = 136/258 (52.71%), Query Frame = 0
Query:  257 RFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQD-VTGKPLLEAYGLTETSPAASI-NPMN-IEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRDL 511
            + T   G  T++  +L       +  +  ++ + GG      V E ++  +  K L+  YG TET PA  + +P +  E    ++G P+   +  + D+NG+ L +   GEL +R P +M GY++   ++ + +  D +  +GD+A + EKG+  I+ RL DMV   G N+YP EIED    H  I+ V +IGVPD+  GE +    +  ++   E DL + C+  +  +KIP+Y  FV + P T  GK+ + E+R +
Sbjct:  655 KCTYLFGTPTMYVAVLEAQKKLKVDVSSVELGITGGSNCLVEVCEDFEKHLNMKSLMNIYGATETGPATFVPSPEDSFEVRINTVGPPLGHVEAKIVDQNGKILPVNTPGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEEL-CAWICLEEGAKEFDLKEHCKGKIAHFKIPKYSMFVKDFPKTLSGKVKKLEMRGI 911          
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Match: EMLSAG00000009904 (supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-1 gene:EMLSAG00000009904 transcript:EMLSAT00000009904 description:"maker-LSalAtl2s645-snap-gene-2.24")

HSP 1 Score: 142.895 bits (359), Expect = 2.696e-36
Identity = 107/374 (28.61%), Postives = 175/374 (46.79%), Query Frame = 0
Query:  162 DDLAFLQYTGGTTGVAKGAMLTHGNMVANVL-QAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLIT--NPRDMLGFIKELRNNRFTIFTGVNTLFAGLL--------NTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIG----------ETGELCIRGPQVMKGYWQRPDATAEVLDQDG-WLSTGDVARMDEKGFVYIVDRLK-DMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLID-FCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRDL 511
            +D A + YT GT+G  KG  +TH N+ A V    + W  TS      DD ++  LPLYH+  L   LL    +G R  ++   + + +  ++     N  ++F GV T+++ L         N     D+  + +++ + G  ++   +  +WQ ++G  LLE YG+TE       +    +   G +G P  + +  +  E G  LA            ++GEL IRGP V   Y+    AT E  D +G W  TGD A M+ +G   I+ R   D++   GF +   EIE    S+  I  + V+G+ DE  GE V  I V +       D++  F  + L  Y +PR    V+ +   ++GKI ++EL+ +
Sbjct:  147 NDGALILYTSGTSGSPKGVWITHRNIEAQVSGMIKFWAWTS------DDHILHVLPLYHLHGLINCLLVPLSIGARVKMLPGFDAKKVWSYLTNSSMN-ISVFMGVPTIYSLLTKEYELDKRNKTEIHDMLRSTYRLMVCGSASLPEPLLRKWQTISGHILLERYGMTEIGMGLGNSLEEDKRLPGFVGTPFPNVEARIMSEEGSVLAYSNDVEFSYLSDKSGELQIRGPSVFSFYYGNEKATKESFDPNGDWFLTGDTASMNSRGVFKILGRTSADIIKSGGFKLSALEIEREYLSYPDISEIAVVGLEDEIFGEIVAAIIVPNKGYYLNSDILRGFGYKKLPPYSVPRQFMLVNSIDKNHMGKINKKELKKM 513          
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Match: EMLSAG00000007052 (supercontig:LSalAtl2s:LSalAtl2s3:1758018:1760492:-1 gene:EMLSAG00000007052 transcript:EMLSAT00000007052 description:"maker-LSalAtl2s3-snap-gene-17.37")

HSP 1 Score: 101.679 bits (252), Expect = 6.595e-23
Identity = 118/468 (25.21%), Postives = 198/468 (42.31%), Query Frame = 0
Query:    6 TFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAH-DDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVR-------TLLITN---------------PRDMLGF----------IKELRNNRFTIFTGV-------------NTLFAGLLNTPGF---KDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGET----GELCIRGPQVMKGYWQRPDATAE-VLDQDG--WLSTGDVARMDEKGFVYIVDRLKDMV 417
            ++ + D   + F   LRH IGL   D++A+     +++ +A   A    + IV       P  + H +  + A+              VI+ + +   LL  L  +    K +++          VP   + ++ +F + + AG+  K  K +    DD+A + YT G+TGV KG +LTH N+V+ +       ST  I   ++D  +  LPL H+  L   ++   R GVR       TLL T+               P  M G           I +  ++R  +F  +             N L   LLN   F     L     K+ + GG  +     +  +   G  +L+ YGLTET+ +A++  +N +   G++G P    D+ + +       + E     GE+ I G  V  GY++ P+ T E  L++ G  W  TGD+  + E G + I+DR KD+V
Sbjct:  162 SYRDCDIIVDNFGRGLRH-IGLCPKDKVAVYAETRAEWFLATQAAFSQNISIVTLYTNLGPEGITHGINQTEAKC-------------VISSNELLPKLLNLLPQLPNVEKVIVIPDHTSITNSKVPEGGV-EIYKFEELVDAGSNWKPAKSEPPQPDDIAIIMYTSGSTGVPKGVLLTHRNLVSTLKSLN---STFEIKISSNDKYIAFLPLAHVLELLCEMMMVIR-GVRIGYSSPNTLLDTSTMIKSGTHGDAAVLQPTVMAGVPLVLTRIYKGISQKMDSRGELFNSIFRFCYDYRLKSIRNGLPTPLLNKILFGKIHKLIGGKVKLIISGGAPLSDEXHDFXRVCLGVDVLQGYGLTETTASATVMDIN-DISTGTVGPPNQFLDIKLVNWEDGNYKVTEQPRPRGEILIGGDCVSNGYYKMPEKTKEDFLEERGKRWFRTGDIGEVTELGTIKIIDRKKDLV 609          
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Match: EMLSAG00000007051 (supercontig:LSalAtl2s:LSalAtl2s3:1688664:1690882:-1 gene:EMLSAG00000007051 transcript:EMLSAT00000007051 description:"augustus_masked-LSalAtl2s3-processed-gene-17.11")

HSP 1 Score: 89.7373 bits (221), Expect = 3.598e-19
Identity = 111/465 (23.87%), Postives = 186/465 (40.00%), Query Frame = 0
Query:    6 TFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAH-DDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVR-------TLLITNPRDMLGF-------------------------IKELRNNRFTIFTGVNTLFAGL--------LNTPGFKDLPFADF--------KVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASI-NPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAE-VLDQDG--WLSTGDVARMDEKGFVYIVDRLKDMV 417
            +F + D     F   LRH IGL   D++A+     +Q  +A LGA    + IV       P  + H +  + A+              VI+ + +   LL  L  +    K +++          VP  ++ K+  F++ + AG+  K VK +    DD+A + YT G+TGV KG +LTH N+V  ++      ST  I + ++D  +  LPL H+  L   ++   R G+R       TLL T+     G                          I +  + R  +F  +   F           L TP    + F  F        K    GG  +   V +  +   G  +++ YGLTET+  A++ +  +I    G+ G P    D          + +    ++  +       Y++  + T E   ++ G  W  TGD+  + E G + I+DR KD+V
Sbjct:  121 SFRDCDIIVENFGRGLRH-IGLCPKDKVAVYAETRAQGLLATLGAFSQNISIVTLYTNLGPEGITHGINQTEAKC-------------VISSNELLPKLLNLLPQLPNVNKVIVIPDHTSISNSQVPEVEV-KIYMFDELVDAGSNWKPVKSEPPQPDDIAIVMYTSGSTGVPKGVLLTHRNLVTTLISIN---STIDIKTSSNDKYIAFLPLAHVLELLCEMMMVIR-GIRIGYSSPNTLLDTSTMIKSGTHGDATVLQPTLMTCVPLVLTRIYNGINQKMDTRGKLFKSIFRFFYDYRLKSIRNGLPTPVLNKILFGKFHKLIGGKVKFIFAGGAPLSDEVHDFIRVCLGVDVIQGYGLTETTACATLMDSSDIS--TGTAGAPNQCID----------IKLVNWEDVFCKFFFFCYSYYKIHEKTKEDFFEEKGKRWFRTGDIGEVTELGTIKIIDRKKDLV 554          
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 87.0409 bits (214), Expect = 2.252e-18
Identity = 113/538 (21.00%), Postives = 212/538 (39.41%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHD---DLAFLQYTGGTTGVAKGAMLTHGNMVANVLQ----AEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGG----GMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGS-------------IGLP--ISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVK-LIAVRSDDSVSEQDLIDFCRESLTGY-KIPRYVQFVDELPMTNVGKILRRELRD 510
            +G    FG V    N  AS L    G  KGD + +   N  +Y I  LG    G +++  N    P EL+ Q+++S  + IL+ D F                      D + F +    N + + +          KV    D L      K V + +  D   D+ ++ +T  ++G AKG + T+ ++++        A  W           D +  A   +H+     N    +  G+    ++   +   F++ L + +    +        L  +P   +   +  KV + G       ++  V +R   +  K + EAYGL E        P   +    S             +GLP   S   +  R +        + GE+C++ PQ M GY  + +++A   D D +  TGD+   D +G VY V+++ +++    + V P+ +E        I+   V+G+P++ +G+  +  + ++     +E++L +     L  + +I   + FV  +P     K+LR  L +
Sbjct:   84 IGRMERFGNVHSLVNKVASGLTKN-GFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTK-ILVCDDFNYV-------------------DGLDFLEN--TNMIEKLIIIGNECTAQDKVILMTDLL--NDDGKKVPQKVIFDWAKDVIYMPFTSTSSG-AKGILHTNKSLMSQFYSPDGAANHWFDQLI-----GDSIHCANYFFHMTGFY-NFALGSIYGISIYTLSEFSEQ-NFLEALVDXKTASISCYAWQIRILSQSPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRIPSI--KYVREAYGLNECGLVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYLNKEESSA--FDNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLRSVLAE 584          
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Match: EMLSAG00000006946 (supercontig:LSalAtl2s:LSalAtl2s393:601207:603901:-1 gene:EMLSAG00000006946 transcript:EMLSAT00000006946 description:"maker-LSalAtl2s393-augustus-gene-5.43")

HSP 1 Score: 86.2705 bits (212), Expect = 4.953e-18
Identity = 118/540 (21.85%), Postives = 212/540 (39.26%), Query Frame = 0
Query:    5 LTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLP--KVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKG-AMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMN---------IEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQD------------GWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGE-AVKLIAVRS-----DDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRDLYGG 514
            LT+ ++ +  + FA  LR    + KGDR+ + MP   +  IA+L   R G +       ++  EL  ++K   A+ ++           +     V+  L+ +    +P         V+ Y ++ +   +L   + + + D ++  +        +  +D  ++ YT GTTG   G A  T+ +M   V     ++  S +   N   V    P     A T +   F+R       +     + GF        F   TG+  L     N       P    K    GG     +  E        P+L+ +  TET+     +PM          +     S G P+   D+ V DENG+ +A GE G +  + P         P   + +   D            G+  T D   ++  G+V  + R  D++ V+G  +  + +E+V  SH  ++   V+GV DE  G+  + LI ++      D S  +++L+   R ++      + +  V+ LP T  GKI R+ + D+  G
Sbjct:  114 LTYSDLKKEVSQFAKVLRDTYDVKKGDRVIIYMPMILETIIAMLAVARLGAIHSVVFGGFSAKELSLRIKHIEAKLVITASCGIEPNRLIKYKPIVDEALIRAEVPELP---------VIVYQRENICQAELKPGRDSEWQDLIEGASVGDLDCVPVLSNDPLYVLYTSGTTGRHPGDAWFTYSDMGWAV--GHGYVCYSPLLHRNTSIVFEGKP-----AGTPDAGQFSR-------VIAENQVKGF--------FLAPTGLRFLKREDPNFEQGSKYPSNSLKAIFVGGERCDNSTKEWASHHFKVPILDHWWQTETA-----HPMTGHCIGLGNTLNPPKDSTGRPVPGFDIRVVDENGKNVANGEMGRIVCKLPIA-------PGCLSTLYGNDEGFVGTYFSRFPGFYDTMDAGXIESNGYVKXLARDDDVINVAGHRLSISRLEEVLISHPDVVDCAVVGVRDELKGQIPIGLIVLKKHFHQEDLSSLKEELVQQVRSNVGAVAXLKILLSVNNLPRTRSGKITRKCIADMAMG 610          
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Match: EMLSAG00000004934 (supercontig:LSalAtl2s:LSalAtl2s259:871050:904452:1 gene:EMLSAG00000004934 transcript:EMLSAT00000004934 description:"augustus_masked-LSalAtl2s259-processed-gene-9.9")

HSP 1 Score: 85.8853 bits (211), Expect = 6.470e-18
Identity = 124/571 (21.72%), Postives = 238/571 (41.68%), Query Frame = 0
Query:    5 LTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLP-----KVTRFNDALKAGATAKAVKEDIA-----HDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTR------LGVRTLLITN---PRDMLGFIKELRNNRFTIFTGV--------------NTLFAGLL----------------NTPGFKDLPFADFKVALG--------GGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYN-GSIGLPISS--TDMCVRDENGQTLAIG--ETGELCIRGPQVMKGYWQRPDATAE-VLDQDG--WLSTGDVARMDEKGFVYIVDRLKDMV-LVSGFNVYPNEIEDVAASH------------EKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLI-DFCRESLTGYKIPRYVQFVDELP 496
            LT+ +V+  +  F+  LRH+  L K D++ +   + +++ ++  G  +  + +V          + H ++ + A+ I+        L++++A                   K  L+  ++   K+     Q        V  FN+ L+ G  + +  ++        +D+A + YT G++GV KG +L+H N+++N+     +L+ + +  + +   +  LPL HIF L+  +  F +       G  TL+ +        +G I EL+     +   +              N  F+ LL                NTP    L F   +  LG        GG  +     E  + V    L + YGLTET     +     E ++ G +G P       +   DE   T+       GE+ + G  +  GY++  D T E   +++G  W  TGD+ ++   G + I+DR KD++ L +G  V   ++E    +H            E+   VG+I +P+E+S + +K      ++SV + +L+    R++L  +   + ++F  E+P
Sbjct:  214 LTYEDVNTISRNFSKGLRHLKFLPK-DKILLYAESSAKWLVSAFGCFKNSMTVVTIYTNLGDEGVYHCIEQTDAKVIITSQNLLKRLQKILAS------------------KGGLIQTIIYLEKEEFSLAQTTPEQDVNVYSFNEILEKGLKSYSQNDNEEDWRPNSEDIAVIMYTSGSSGVPKGVVLSHFNIMSNIFGIMSFLNQNFVRLDKES-YIGYLPLAHIFELSVEIALFGKGIPIGFSGPNTLMNSGSMVKEGQIGDICELKPTYMAVVPLIADRIYKIIIQKVSENGPFSELLFNFCYEYKSKWRKLGMNTPIINLLFFKKIQQFLGGNVSTIVSGGAPLSAQAHEFMEIVFDLKLSQGYGLTETCGGFILGRK--EDFSLGEVGHPFPGPFVKLTNWDEGEYTVKDKPYPRGEIHVGGGLIAMGYFELMDETEENFYEENGVRWFKTGDIGQLQSSGALKIIDRKKDIIKLQNGEYVSLGKVETYLGTHPLVDIICVYGEPERNCIVGLI-IPNEKSIKILKESLGLRNESVYDNNLLRSQVRQALANHSEAKLLKF--EIP 759          
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Match: EMLSAG00000012891 (supercontig:LSalAtl2s:LSalAtl2s981:104426:107346:-1 gene:EMLSAG00000012891 transcript:EMLSAT00000012891 description:"maker-LSalAtl2s981-augustus-gene-1.58")

HSP 1 Score: 85.5001 bits (210), Expect = 9.468e-18
Identity = 131/555 (23.60%), Postives = 226/555 (40.72%), Query Frame = 0
Query:    6 TFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQL-----PKVTRFNDALKAGATAKAVKEDI--AHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGM--AVQRAVAERWQDVTGKPLL-EAYGLTETSPAASINPMNIEGYNGSIGL--------------PIS------STDMCVRDENGQTLA--IGETGE---LCIRGPQV--MKGYWQRPDATAEVLDQ-----DGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAAS-HEKILSVGVIGVP----DERSGEAVKLIAVRSDDSVSEQ---DLIDFCRESLTGYKIPRYVQFV-DELPMTNVGKILRRELR 509
            TF  +DQ ++  A+Y  + +GL KGD +A+ M N  ++    LG  + GV+    N       + + LK   A+A+    ++   L++ + +      +  SL D+ P       N  +   K  V    +     P    F++ +      +   ++I  +   + F  YT GTTG+ K   +TH   +  ++  +  L  +     ++D     LPLYH   +   +      G + ++I        F K+    + T    +  +   LL T    +      ++  G G+   + R+  ER++     PL+ E YG TE     ++N +N +   G++G               PI        T    RDENG  +   +GE GE     ++G  V    GY    +   ++L       D +  +GD+   DE G++Y  DR+ D     G NV   E+E  A S  +    V V GV     D R+G    +IA+ S D   ++    L D  +  L  Y  P +V+ +  E+ +T   KI +  L+
Sbjct:  147 TFEMIDQESSRVANYFSNNVGLKKGDCVALYMTNRPEFIYITLGLAKIGVIAALVNSHLRQDSMVYALKAGNAKAL----IYGIELQDAVDE------MEKSLLDLSP-------NFTLYCSKSGVSPEDITRKRSPVSKDFDEIVSKSPANRGASQNISLSPSSVIFYIYTSGTTGLPKPVKITHMRQMVILMAIKNLLDLN-----SEDTSYCYLPLYHASGIQLGIGNAFANGGK-VVIKKKFSARNFWKDCVKYKVTATQYIGEICRFLLATKPIPEETQHSIRIIYGNGLRPEIWRSFVERFK----IPLVGEFYGSTE----GNMNLINFDNTVGAVGCIPNIIPKFLIELITPIHLIKVNPQTLEVYRDENGLCVQCDVGEIGEGIGRIVKGHPVKEFNGYANEEENGNKILRDVLKKGDIFFRSGDLLVQDELGYLYFKDRVGDTFRWKGENVSTYEVEMTANSLLDNQTEVAVYGVKVSGMDGRAG----MIALPSKDEDVKKLLDKLFDGFKSKLPLYARPVFVRLLHSEMALTATYKIKKFSLK 666          
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 82.4185 bits (202), Expect = 6.204e-17
Identity = 60/213 (28.17%), Postives = 106/213 (49.77%), Query Frame = 0
Query:  305 DVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDEN-GQTLAIGETGELCIRGPQVMK-GYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIED-VAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYK-IPRYVQFVDE-LPMTNVGKILRRELRDLY 512
            +V  K +   YG TE+     ++    +  NG +   ++ T+  +RD N GQ L   + GEL  R       GY  R +   ++ ++DGW  TGD     E+G  +   R+ +M+   G +VYP E+E  + A H+ I+ VGV+ V +++      +I V++D  ++ + +     + +  YK I   +    E LP   +GK+LR E++ L+
Sbjct:  280 EVLKKCVPNFYGSTESGGGFLVSTN--QHSNGGV---VARTETYIRDLNTGQHLGPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVVPVVEKKQTVLGAIIVVKNDKKINVETIRKELNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEIQKLF 487          
BLAST of long-chain fatty acid-- ligase vs. SwissProt
Match: gi|24637994|sp|Q8ZES9.1|LCFA_YERPE (RecName: Full=Long-chain-fatty-acid--CoA ligase; AltName: Full=Long-chain acyl-CoA synthetase)

HSP 1 Score: 600.897 bits (1548), Expect = 0.000e+0
Identity = 295/510 (57.84%), Postives = 380/510 (74.51%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
            MG  +TF ++++ + AFA+YL+  +GL KGDR+A+MMPN  QYPIA+ G LRAG+++VN NPLYTP EL+HQL DSGA AI+I+  FA TLE+V+    V HV+LT +GD +   K  LVN VV+Y+K++VP Y LP    F   L+ G   + VK D+ + D AFLQYTGGTTGVAKGA+LTH NM +N+ QA+   + + +     D VVTALPLYHIFALT N L F  LG R+LLITNPRD+ G +KEL    FT  TGVNTLF  LLN   F  L F+  ++++GGGM VQ+AVAE+W+ +TGK LLE YGLTE SP  + NP +++ Y+GSIGLP+ STD+ +RD++G  + +G+ GEL +RGPQVM GYWQRPDAT +VL +DGWL+TGD+A MDE GF+ IVDR KDM+LVSGFNVYPNEIE+V A H K+L   VIGVP+E SGEAVK+  V++D S++ ++L+  CR  LTGYK+P+ V+F DELP +NVGKILRRELRD
Sbjct:   45 MGEVMTFRKLEERSRAFAAYLQQGLGLQKGDRVALMMPNLLQYPIALFGVLRAGMIVVNVNPLYTPRELEHQLSDSGAVAIVIVSNFAHTLEKVVFKTQVRHVILTRMGDQLSAAKGTLVNFVVKYIKRLVPKYYLPDAISFRTVLQKGRRMQYVKPDVINTDTAFLQYTGGTTGVAKGAILTHRNMQSNLEQAKA--AYAPLLQPGRDLVVTALPLYHIFALTVNCLLFIELGGRSLLITNPRDIPGMVKELSRYPFTAITGVNTLFNALLNNEEFTHLDFSTLRLSVGGGMPVQKAVAEKWETLTGKHLLEGYGLTECSPLVTGNPYDLKHYSGSIGLPVPSTDVRLRDDDGNDVELGKPGELWVRGPQVMLGYWQRPDATDDVL-KDGWLATGDIATMDEDGFLRIVDRKKDMILVSGFNVYPNEIEEVVALHAKVLESAVIGVPNEVSGEAVKVFVVKNDASLTPEELLTHCRRYLTGYKVPKIVEFRDELPKSNVGKILRRELRD 551          
BLAST of long-chain fatty acid-- ligase vs. SwissProt
Match: gi|24637992|sp|Q8XDR6.1|LCFA_ECO57 (RecName: Full=Long-chain-fatty-acid--CoA ligase; AltName: Full=Long-chain acyl-CoA synthetase; Short=Acyl-CoA synthetase)

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 289/511 (56.56%), Postives = 372/511 (72.80%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSG-ISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
            MG  +TF ++++ + AFA+YL+  +GL KGDR+A+MMPN  QYP+A+ G LRAG+++VN NPLYTP EL+HQL DSGA AI+I+  FA TLE+V+    V+HV+LT +GD +   K  LVN VV+Y+K++VP Y LP    F  AL  G   + VK ++  +DLAFLQYTGGTTGVAKGAMLTH NM+AN+ Q     +T G +     + VVTALPLYHIFALT N L F  LG + LLITNPRD+ G +KEL    FT  TGVNTLF  LLN   F+ L F+   ++ GGGM VQ+ VAERW  +TG+ LLE YGLTE +P  S+NP +I+ ++GSIGLP+ ST+  + D++   ++ G+ GELC+RGPQVM GYWQRPDAT E++ ++GWL TGD+A MDE+GF+ IVDR KDM+LVSGFNVYPNEIEDV   H  +  V  +GVP   SGEAVK+  V+ D S++E+ L+ FCR  LTGYK+P+ V+F DELP +NVGKILRRELRD
Sbjct:   45 MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAKGTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVN---ATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELGGQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLVDDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEII-KNGWLHTGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSLTEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRD 551          
BLAST of long-chain fatty acid-- ligase vs. SwissProt
Match: gi|54037718|sp|P63522.1|LCFA_SALTI (RecName: Full=Long-chain-fatty-acid--CoA ligase; AltName: Full=Long-chain acyl-CoA synthetase >gi|54041251|sp|P63521.1|LCFA_SALTY RecName: Full=Long-chain-fatty-acid--CoA ligase; AltName: Full=Long-chain acyl-CoA synthetase)

HSP 1 Score: 589.341 bits (1518), Expect = 0.000e+0
Identity = 289/511 (56.56%), Postives = 371/511 (72.60%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSG-ISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
            MG  +TF ++++ + AFA+YL+  +GL KGDR+A+MMPN  QYP+A+ G LRAG+++VN NPLYTP EL+HQL DSGA AI+I+  FA TLE+V+    V+HV+LT +GD +   K  +VN VV+Y+K++VP Y LP    F  AL+ G   + VK ++  +DLAFLQYTGGTTGVAKGAMLTH NM+AN+ Q +   +T G +     + VVTALPLYHIFALT N L F  LG + LLITNPRD+ G +KEL    FT  TGVNTLF  LLN   F+ L F+   ++ GGGM VQ  VAERW  +TG+ LLE YGLTE +P  S+NP +I+ ++GSIGLP+ ST+  + D++   +A GE GELC++GPQVM GYWQRPDAT E++ +DGWL TGD+A MDE GF+ IVDR KDM+LVSGFNVYPNEIEDV   H  +  V  +GVP   SGEAVKL  V+ D ++++  LI FCR  LTGYK+P+ V+F +ELP +NVGKILRRELRD
Sbjct:   45 MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAAAIIIVSNFAHTLEKVVEKTSVQHVILTRMGDQLSTAKGTVVNFVVKYIKRLVPKYHLPDAISFRSALQHGYRMQYVKPEVVAEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVK---ATYGPLLHPGKELVVTALPLYHIFALTMNCLLFIELGGQNLLITNPRDIPGLVKELAKYPFTAMTGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQNVVAERWVKLTGQYLLEGYGLTECAPLVSVNPHDIDYHSGSIGLPVPSTEAKLVDDDDNEVAPGEAGELCVKGPQVMLGYWQRPDATDEII-KDGWLHTGDIAVMDEDGFLRIVDRKKDMILVSGFNVYPNEIEDVVMQHSGVQEVAAVGVPSGSSGEAVKLFVVKKDPALTDDALITFCRRHLTGYKVPKQVEFREELPKSNVGKILRRELRD 551          
BLAST of long-chain fatty acid-- ligase vs. SwissProt
Match: gi|60392603|sp|P69451.1|LCFA_ECOLI (RecName: Full=Long-chain-fatty-acid--CoA ligase; AltName: Full=Long-chain acyl-CoA synthetase; Short=Acyl-CoA synthetase >gi|60392604|sp|P69452.1|LCFA_ECOL6 RecName: Full=Long-chain-fatty-acid--CoA ligase; AltName: Full=Long-chain acyl-CoA synthetase; Short=Acyl-CoA synthetase)

HSP 1 Score: 587.03 bits (1512), Expect = 0.000e+0
Identity = 287/511 (56.16%), Postives = 371/511 (72.60%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSG-ISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
            MG  +TF ++++ + AFA+YL+  +GL KGDR+A+MMPN  QYP+A+ G LRAG+++VN NPLYTP EL+HQL DSGA AI+I+  FA TLE+V+    V+HV+LT +GD +   K  +VN VV+Y+K++VP Y LP    F  AL  G   + VK ++  +DLAFLQYTGGTTGVAKGAMLTH NM+AN+ Q     +T G +     + VVTALPLYHIFALT N L F  LG + LLITNPRD+ G +KEL    FT  TGVNTLF  LLN   F+ L F+   ++ GGGM VQ+ VAERW  +TG+ LLE YGLTE +P  S+NP +I+ ++GSIGLP+ ST+  + D++   +  G+ GELC++GPQVM GYWQRPDAT E++ ++GWL TGD+A MDE+GF+ IVDR KDM+LVSGFNVYPNEIEDV   H  +  V  +GVP   SGEAVK+  V+ D S++E+ L+ FCR  LTGYK+P+ V+F DELP +NVGKILRRELRD
Sbjct:   45 MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAKGTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVN---ATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELGGQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLVDDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEII-KNGWLHTGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSLTEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRD 551          
BLAST of long-chain fatty acid-- ligase vs. SwissProt
Match: gi|1170729|sp|P46450.1|LCFA_HAEIN (RecName: Full=Long-chain-fatty-acid--CoA ligase; AltName: Full=Long-chain acyl-CoA synthetase)

HSP 1 Score: 575.859 bits (1483), Expect = 0.000e+0
Identity = 275/511 (53.82%), Postives = 375/511 (73.39%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSEN-DDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
            MG  LTF ++++ + AFA+YL++   L +GDR+A+MMPN  QYPIA+ G LRAG++ VN NPLYTP EL+ QL+DSGA AI+++  FA+TLE+V+ +  V+HV+LT +GD + F KR LVN VV+YVKK+VP Y+LP    F + L  G   + V+ +I+ +DLAFLQYTGGTTGVAKGAMLTHGN++ NV QA +W++   I   +     + ALPLYH+FALT N L F  LGV  +LITNPRD+ GF+KEL+  RF   TGVNTLF  LLN   FK++ F+  K+++GGGMA+Q++VA RW ++TG  ++E YG+TE SP  +  P+N+  +NG+IG+P+ +TD+ +  ++G    IGE GEL ++G QVM+GYWQRP+AT+EVL +DGW++TGD+  MDE   + IVDR KD++LVSGFNVYPNEIEDV   + K+     IGVP   SGE +K+  V+ DDS++  +L + CR+ LTGYK+P+ ++F DELP TNVGKILRR LRD
Sbjct:   45 MGQVLTFRKLEERSRAFAAYLQNEFKLQRGDRVALMMPNLLQYPIALFGILRAGLIAVNVNPLYTPRELELQLQDSGAVAIVVVSNFASTLEKVVFNTNVKHVILTRMGDQLSFGKRTLVNFVVKYVKKLVPKYKLPHAVTFREVLSIGKYRQYVRPEISREDLAFLQYTGGTTGVAKGAMLTHGNIITNVFQA-KWIAEPFIGDHSRTRSAILALPLYHVFALTVNCLLFLELGVTAILITNPRDIEGFVKELKKYRFEAITGVNTLFNALLNNENFKEVDFSALKLSVGGGMAIQQSVATRWHELTGCNIIEGYGMTECSPLIAACPINVVKHNGTIGVPVPNTDIKIIKDDGSDAKIGEAGELWVKGDQVMRGYWQRPEATSEVL-KDGWMATGDIVIMDESYSLRIVDRKKDIILVSGFNVYPNEIEDVVMLNYKVSEAVAIGVPHAVSGETIKIFVVKKDDSLTRDELRNHCRQYLTGYKVPKEIEFRDELPKTNVGKILRRVLRD 553          
BLAST of long-chain fatty acid-- ligase vs. SwissProt
Match: gi|2829810|sp|P94547.1|LCFA_BACSU (RecName: Full=Long-chain-fatty-acid--CoA ligase; AltName: Full=Long-chain acyl-CoA synthetase)

HSP 1 Score: 351.673 bits (901), Expect = 4.215e-114
Identity = 201/511 (39.33%), Postives = 292/511 (57.14%), Query Frame = 0
Query:    2 GNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATA--KAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCV-RDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLID-FCRESLTGYKIPRYVQFVDELPMTNVGKILRREL 508
            G KLTF ++   A   A++L+   GL KGDR+A+M+PNC Q  I+  G L AG ++V TNPLYT  EL++QL+D+    I+ +DL      ++     V+ +L+TS+ D +PFPK +L   + +  K  +   +   +  F   +K   T      K D  HD +A LQYTGGTTG  KG MLTH N++AN      W+       E  + V+  +P +H++ LTA +    +LG   +L+    D L  +K +  ++ T+F G  T++ GLL+ P  +    +  K  L G  A+   V ++++ VTG  L+E YGL+E SP    N +  +   GSIG P  STD  +  +E G+  A  E GE+ ++GPQVMKGYW +P+ TA VL +DGWL TGD+  MDE+GF YI DR KD+++  G+N+YP E+E+    HE I  + V GVPD   GE VK   V    + ++ + +D F R  L  YK+P+  +F  ELP T VGKILRR L
Sbjct:   47 GKKLTFHDILTDALKLAAFLQ-CNGLQKGDRVAVMLPNCPQTVISYYGVLFAGGIVVQTNPLYTEHELEYQLRDAQVSVIITLDLLFPKAIKMKTLSIVDQILITSVKDYLPFPKNILY-PLTQKQKVHIDFDKTANIHTFASCMKQEKTELLTIPKIDPEHD-IAVLQYTGGTTGAPKGVMLTHQNILANTEMCAAWMYDV---KEGAEKVLGIVPFFHVYGLTAVMNYSIKLGFEMILLPK-FDPLETLKIIDKHKPTLFPGAPTIYIGLLHHPELQHYDLSSIKSCLSGSAALPVEVKQKFEKVTGGKLVEGYGLSEASPVTHANFIWGKNKPGSIGCPWPSTDAAIYSEETGELAAPYEHGEIIVKGPQVMKGYWNKPEETAAVL-RDGWLFTGDMGYMDEEGFFYIADRKKDIIIAGGYNIYPREVEEALYEHEAIQEIVVAGVPDSYRGETVKAFVVLKKGAKADTEELDAFARSRLAPYKVPKAYEFRKELPKTAVGKILRRRL 549          
BLAST of long-chain fatty acid-- ligase vs. SwissProt
Match: gi|239938656|sp|O07610.2|LCFB_BACSU (RecName: Full=Long-chain-fatty-acid--CoA ligase; AltName: Full=Long-chain acyl-CoA synthetase)

HSP 1 Score: 319.316 bits (817), Expect = 4.298e-102
Identity = 190/508 (37.40%), Postives = 278/508 (54.72%), Query Frame = 0
Query:    5 LTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHP-VEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRDL 511
            +T+ E+++Y   FA  L+   G+ KGD +A+++ N   + IA  GAL+AG+V+V  NPLYTPTE+ + L +   +AI+ +       E +    P VE V+L   G+  P              +   P  ++ K+T F   L+  + AK  +E +  DD A + YT GTTG  KGAMLTH N+ +N      +L       +  D VV ALP++H+F LT  +      G  T+LI          K ++  + TIF GV T++  L      K   F+  ++ + GG ++  A+   +++  G  +LE YGL+E SP    NP +     GSIG  I   +  V D  G+ L   + GEL ++GP VMKGY++ P  T   L +DGWL TGD+AR DE G+ YIVDR KDM++V G+NVYP E+E+V  SH  +    VIGVPD +SGEAVK   V     V+E+D++  C + L  YK P  + F+D++P    GK+LRR LRD+
Sbjct:   27 MTYQELNEYIQRFADGLQEA-GMEKGDHLALLLGNSPDFIIAFFGALKAGIVVVPINPLYTPTEIGYMLTNGDVKAIVGVSQLLPLYESMHESLPKVELVILCQTGEAEP--------------EAADPEVRM-KMTTFAKILRPTSAAKQNQEPVP-DDTAVILYTSGTTGKPKGAMLTHQNLYSNANDVAGYLGM-----DERDNVVCALPMFHVFCLTVCMNAPLMSGA-TVLIEPQFSPASVFKLVKQQQATIFAGVPTMYNYLFQHENGKKDDFSSIRLCISGGASMPVALLTAFEEKFGVTILEGYGLSEASPVTCFNPFDRGRKPGSIGTSILHVENKVVDPLGRELPAHQVGELIVKGPNVMKGYYKMPMETEHAL-KDGWLYTGDLARRDEDGYFYIVDRKKDMIIVGGYNVYPREVEEVLYSHPDVKEAVVIGVPDPQSGEAVKGYVVPKRSGVTEEDIMQHCEKHLAKYKRPAAITFLDDIPKNATGKMLRRALRDI 510          
BLAST of long-chain fatty acid-- ligase vs. SwissProt
Match: gi|75075869|sp|Q4R4Z9.1|ACSF2_MACFA (RecName: Full=Acyl-CoA synthetase family member 2, mitochondrial; Flags: Precursor)

HSP 1 Score: 237.269 bits (604), Expect = 1.257e-69
Identity = 166/521 (31.86%), Postives = 255/521 (48.94%), Query Frame = 0
Query:    4 KLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATA------KAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTG----KPLLEAYGLTETSPAASIN-PMN-IEGYNGSIGLPISSTDMCVRD-ENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAV-KLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
            +LTF ++ +  +  AS L   IGL KGDR+ M  PN   + +  L   +AG+++V+ NP Y  TEL++ LK  G +A++    F       I       V     G +    +RL     V  V   +P   L      ++ L AG+T       + +++ ++  D   +Q+T GTTG  KGA L+H N+V N     E L     + E    ++ + PLYH        +     G   +L +   +    ++ +   R +   G  T+F  +LN P F      D     GG +A   A  E  + +      K L+ AYG TE SP    N P + +E    S+G  +  T+  + + E G    +   GELCIRG  VM GYW  P  T E +DQD W  TGD+A M+E+GF  IV R KDM++  G N+YP E+ED   +H K+  V V+GV D+R GE +   I ++  +  + +++  FC+  ++ +KIPRY+ FV   P+T  GKI + +LR+
Sbjct:  102 RLTFAQLKEEVDKAASGLLS-IGLCKGDRLGMWGPNSYAWVLIQLATAQAGIILVSVNPAYQATELEYVLKKVGCKALVFPKQFKTQQYYNILKQICPEVENAQPGALK--SQRLPDLTTVISVDAPLPGTLL-----LDEVLAAGSTQQHLEQLQHIQQFLSCHDPINIQFTSGTTGSPKGATLSHYNIVNNSSILGERLKLHEKTPEQLRMILPS-PLYHCLGSVGGTMMCLMYGATLILASPVFNGKKALEAISRERGSFLYGTPTMFVDILNQPDFSSY---DISTMRGGVIAGSPAPPELIRAIINKINMKDLVVAYGTTENSPVTFANFPEDTVEQKAESVGRIMPHTEARIMNMEAGMLAELNTPGELCIRGYCVMLGYWGEPQKTGEAVDQDKWYRTGDIATMNEQGFCKIVGRSKDMIIRGGENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTAEEMKAFCKGKISHFKIPRYIVFVTNYPLTTSGKIQKFKLRE 610          
BLAST of long-chain fatty acid-- ligase vs. SwissProt
Match: gi|123781622|sp|Q499N5.1|ACSF2_RAT (RecName: Full=Acyl-CoA synthetase family member 2, mitochondrial; Flags: Precursor)

HSP 1 Score: 236.884 bits (603), Expect = 1.774e-69
Identity = 167/523 (31.93%), Postives = 259/523 (49.52%), Query Frame = 0
Query:    9 EVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAAT-----LEEVIAD----HP----VEHV--LLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPR-DMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVA-LGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASIN-PMN-IEGYNGSIGLPISSTD-MCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAV-KLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
            EVD+ A+   S     IGL KGDR+ M  PN   + +  L   +AG+++V+ NP Y  +EL++ L+  G + I+    F        L++V  +     P     E +  L T +    P P  LL++ VV    K     QL    R++               ++  D   +Q+T GTTG  KGA L+H N+V N     + L     ++E    V+   PLYH        +     G  TLL+++P  +    ++ +   + T+  G  T+F  +LN P F    F   +   + G +A    +      +  K L+  YG TE SP   +N P + +E   GS+G  +  T+   V  E G+   +   GELCIRG  VM+GYW  P  T E + QD W  TGD+A MDE+GF  IV R KDM++  G N+YP E+ED    H ++    V+GV D+R GE +   I ++S ++ +E+++  FC+  ++ +KIPRY+ FV+  P+T  GKI + +LR+
Sbjct:  108 EVDRAASGLLS-----IGLRKGDRLGMWGPNSYAWVLIQLATAQAGIILVSVNPAYQASELEYVLRKVGCKGIVFPKQFKTQQYYNILKQVCPELEKAQPGALKSERLPDLTTVISVDAPLPGTLLLDEVVAAGGKEQNLAQL----RYHQGF------------LSCYDPINIQFTSGTTGNPKGATLSHHNIVNNSNLIGQRLKMPAKTAEELRMVLPC-PLYHCLGSVGGTMVSVVHGA-TLLLSSPSFNGKKALEAISREKGTLLYGTPTMFVDILNQPDFSSYDFTTIRGGVIAGSLAPPELIRAIISKMNMKELVVVYGTTENSPVTFMNFPEDTLEQKAGSVGRIMPHTEAQIVNMETGELTKLNMPGELCIRGYCVMQGYWGEPQKTFETVGQDRWYRTGDIASMDEQGFCRIVGRSKDMIIRGGENIYPAELEDFFHKHPQVQEAQVVGVKDDRMGEEICACIRLKSGETTTEEEIKAFCKGKISHFKIPRYIVFVEGYPLTVSGKIQKFKLRE 607          
BLAST of long-chain fatty acid-- ligase vs. SwissProt
Match: gi|81669033|sp|O31826.1|YNGI_BACSU (RecName: Full=Putative acyl-CoA synthetase YngI)

HSP 1 Score: 234.958 bits (598), Expect = 2.449e-69
Identity = 165/524 (31.49%), Postives = 254/524 (48.47%), Query Frame = 0
Query:    4 KLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDM----MPFPKRLLVNAVVRYV--KKMVPAYQLPKVTRFNDALKAGATAKAVKE---DIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGM----AVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYN--GSIGLPISSTDM-CVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSG-EAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
            + T+ + D      A  L   +G+ KGD +A+   N S++        + G V+V  N  Y   EL + LK S A A++IMD +  T    I +  +  +     G +     PF K L+      Y+  K++   Y         + L    T   ++E    +  D++  +QYT GTTG  KG MLTH N++ N     E ++   ++S++  C+   +P +H F     +L    +G   ++     D +  +K +   + T+  GV T+F   L+ P F     +  +  +  G      V +AV ER   +  K +  AYG TE SP  +    N        + G  +  T++  V     Q +  G  GELC RG  VMKGY++  DAT + ++ DGWL TGD+A MDE G+  I  RLKDM++  G N+YP EIE+    H  +L V V+GVPD + G EA   I ++   SVS  +L  +C+  +  +KIPRYV F D+ PMT  GKI + +LR+
Sbjct:   34 RYTYAQFDSLCRQTAKGLMR-MGIGKGDHVAIWASNISEWLAVQFATAKIGAVLVTVNTNYQAHELDYLLKQSDAAALIIMDSYRGTSYPDIVNSLIPELQEAKPGQLKSERYPFLKTLI------YIGNKRLSGMYHWDD----TEILAKTVTDAELEERMNSLDKDNVINMQYTSGTTGFPKGVMLTHFNVINNAANIAECMA---LTSQDRMCI--PVPFFHCFGCVLGVLACVSVGA-AMIPVQEFDPVTVLKTVEKEKCTVLHGVPTMFIAELHHPDFDAYDLSTLRTGIMAGSPCPSEVMKAVIER---MGMKDITIAYGQTEASPVITQTRANDSFIRRVETTGRALPHTEVKIVEPGTCQEVQRGMQGELCTRGYHVMKGYYKDKDATRKAINHDGWLFTGDLAVMDEDGYCRITGRLKDMLIRGGENIYPREIEEFLYQHPAVLDVQVVGVPDAKFGEEAAAWIKLKDGKSVSPDELKAYCKGKIARHKIPRYVIFTDDYPMTASGKIQKYKLRE 537          
BLAST of long-chain fatty acid-- ligase vs. nr
Match: gi|906867123|ref|WP_049725553.1| (long-chain fatty acid--CoA ligase [Wenzhouxiangella marina] >gi|906396522|gb|AKS41951.1| Long-chain fatty acid--CoA ligase [Wenzhouxiangella marina])

HSP 1 Score: 687.567 bits (1773), Expect = 0.000e+0
Identity = 338/517 (65.38%), Postives = 406/517 (78.53%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRDLYGGQQS 517
             G +L++ +VD Y+ AF + ++  +GL KGDR+A+MMPN  QYPIAV GALRAG+V+VNTNPLYT  ELKHQLKDSGARAI+IMD FA+ LE V+ D  VEHV+LT +GDM+   K  ++N V+R VKKMVP Y LP    F   L  GA A   +  + HDDLAFLQYTGGTTGV+KGAMLTHGNMVAN+ Q+  WL +  + +  ++ +VTALPLYHIFALTAN L F + G R LLITNPRDM GF+KEL + RFT  TGVNTLF GLLNTPGF DL F+  ++ LGGGMAVQRAVAERW+  TG PL+EAYGLTETSPAA INPM+++ YNG+IGLPI ST   V D +GQ +A GETGELC+RGPQVMKGYWQRP+ TA+VL +DGWL TGD+ARMDE G+ YIVDR KDM+LVSGFNVYPNEIEDV A H K+L VG IG PDE+SGE VK++ VR D S++ ++L + CR+ LTGYK+P+YV+FVDELP TNVGKILRRELRD YG  +S
Sbjct:   46 FGAELSYKQVDDYSRAFGAQMQ-ALGLGKGDRIAIMMPNVLQYPIAVFGALRAGLVVVNTNPLYTARELKHQLKDSGARAIVIMDNFASVLEAVVDDTQVEHVILTRMGDMVGGLKGAIMNFVIRRVKKMVPPYSLPGSLTFKAVLADGARASLDEPALNHDDLAFLQYTGGTTGVSKGAMLTHGNMVANMQQSSAWLGS--LVTPGEETIVTALPLYHIFALTANCLVFLKFGGRNLLITNPRDMAGFVKELGSIRFTAITGVNTLFNGLLNTPGFADLDFSSLRLTLGGGMAVQRAVAERWKKTTGVPLIEAYGLTETSPAACINPMDLKDYNGAIGLPIPSTSCQVVDPDGQPVAQGETGELCVRGPQVMKGYWQRPEETAKVLTEDGWLRTGDMARMDENGYFYIVDRKKDMILVSGFNVYPNEIEDVVADHPKVLEVGAIGAPDEKSGEVVKVVVVRKDPSLTVKELREHCRKELTGYKVPKYVEFVDELPKTNVGKILRRELRDQYGDPRS 559          
BLAST of long-chain fatty acid-- ligase vs. nr
Match: gi|1148783658|ref|WP_077513564.1| (long-chain-fatty-acid--CoA ligase [Rhodanobacter sp. B05] >gi|1147851752|gb|OOG55845.1| long-chain-fatty-acid--CoA ligase [Rhodanobacter sp. B05])

HSP 1 Score: 681.789 bits (1758), Expect = 0.000e+0
Identity = 322/510 (63.14%), Postives = 399/510 (78.24%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
             G KL++G++D  +  FA YL  V+ L KGDR+A+MMPN  QYPIA+ GALRAG+V+VNTNPLYT  EL+HQL+DSGA+AI+++D FAATL++V+A+  V+H++ T LGDM+ FPK  L+N  ++++KKMVPA+ LP+  RF DAL  GAT       +AHDDLAFLQYTGGTTGVAKGAMLTHGNMVAN+LQA+ W+S S  +   ++ ++TALPLYHIF+LTAN L FTRLG    LITNPRDM GF+KEL+ + FT  TGVNTLF GLLNTPGF DL F+   + LGGGMAVQRAVAERW+ VTG  L EAYGLTETSPA  INP++++ YNGSIGLP+ ST++ +  E+G+ L +GE GEL + GPQVMKGYW RPD TA+VL  DGWL TGD+A+MDEKG+ YIVDR KDM+LVSGFNVYPNE+EDV   H  +L V  +GVPDE SGE VKL  VR D +++ +DL  FC + LTGYK PR ++F +ELP +NVGKILRRELRD
Sbjct:   47 FGRKLSYGQIDDMSRQFAGYLTGVLKLGKGDRIAIMMPNVMQYPIALFGALRAGLVVVNTNPLYTARELRHQLQDSGAKAIVVLDNFAATLQQVVAETSVQHIVTTGLGDMLGFPKGALINFALKHIKKMVPAFNLPQAVRFRDALAQGATHPLQPVALAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANMLQADAWISAS--TKVGEEVIITALPLYHIFSLTANGLVFTRLGGLNWLITNPRDMPGFVKELKASSFTALTGVNTLFNGLLNTPGFADLDFSRLHMTLGGGMAVQRAVAERWKKVTGCTLAEAYGLTETSPAVCINPLDLKEYNGSIGLPVPSTNVAIWSEDGKPLLVGEVGELMVHGPQVMKGYWNRPDETAKVLGADGWLHTGDIAKMDEKGYFYIVDRKKDMILVSGFNVYPNEVEDVVMEHPGVLEVAAVGVPDEHSGEVVKLFVVRKDPNLTVEDLKQFCHDRLTGYKRPRQIEFRNELPKSNVGKILRRELRD 554          
BLAST of long-chain fatty acid-- ligase vs. nr
Match: gi|947300907|ref|WP_056007295.1| (long-chain fatty acid--CoA ligase [Frateuria sp. Soil773] >gi|946438875|gb|KRE89194.1| long-chain fatty acid--CoA ligase [Frateuria sp. Soil773])

HSP 1 Score: 681.404 bits (1757), Expect = 0.000e+0
Identity = 324/510 (63.53%), Postives = 397/510 (77.84%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
             G +L++GE+DQ +  FA YL  V+ L KGDR+A+MMPN  QYPIA+ GALRAG+V+VNTNPLYT  ELKHQL+DSGA+AI+++D FAATL++V+A+  VEH++ T +GDM+ FPK  LVN V+++VKKMVPA++LP   RF DAL  GA     K ++ H DLAFLQYTGGTTGVAKGAML+HGNMVAN+LQA  W+ T  +    ++ ++TALPLYHIF+LTAN L F RLG    LITNPRDM GF+KEL+ +RFT  TGVNTLF GLLNTPGF +L F+   + LGGGMAVQRAVAERW+ VTG  L EAYGLTETSPA  INP++++ YNGSIGLP+ ST++ +  E+ Q LA GE GEL +RGPQVM+GYWQRPD TA+VL  DGWL TGD+ARMDE G+ YIVDR KDM+LVSGFNVYPNE+ED    H  +  V  +GVPDE SGE VKL  VR D +++E+ L  FCRE+LTGYK P+ ++F D LP TNVGKILRRELRD
Sbjct:   47 FGKQLSYGEIDQLSRRFAGYLTGVLKLGKGDRIAIMMPNVLQYPIALFGALRAGLVVVNTNPLYTGRELKHQLEDSGAKAIVVLDNFAATLQQVLAETHVEHIVTTGIGDMLGFPKGALVNFVLKHVKKMVPAFKLPHAVRFRDALARGAAHPLPKVELGHADLAFLQYTGGTTGVAKGAMLSHGNMVANMLQAGAWIGTD-LVKPGEEVIITALPLYHIFSLTANGLVFMRLGGLNWLITNPRDMPGFVKELKQSRFTALTGVNTLFNGLLNTPGFAELDFSRLHLTLGGGMAVQRAVAERWKKVTGCTLAEAYGLTETSPAVCINPLDLKAYNGSIGLPVPSTEVAIWSEDNQPLATGEVGELMVRGPQVMQGYWQRPDETAKVLGADGWLHTGDIARMDENGYFYIVDRKKDMILVSGFNVYPNEVEDAVMLHPGVAEVAAVGVPDEHSGEVVKLFVVRKDPNLTEEALKQFCRENLTGYKRPKLIEFRDALPKTNVGKILRRELRD 555          
BLAST of long-chain fatty acid-- ligase vs. nr
Match: gi|946850140|ref|WP_055773187.1| (MULTISPECIES: long-chain fatty acid--CoA ligase [Stenotrophomonas] >gi|944743558|gb|KQN94616.1| long-chain fatty acid--CoA ligase [Stenotrophomonas sp. Leaf70] >gi|947001701|gb|KRG55352.1| long-chain fatty acid--CoA ligase [Stenotrophomonas nitritireducens])

HSP 1 Score: 679.863 bits (1753), Expect = 0.000e+0
Identity = 324/510 (63.53%), Postives = 395/510 (77.45%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
             G  LT+G+ D     FASYL   + L KGDR+A+MMPNC QYPIA  G LRAG+ +VN NPLYT  ELKHQL D+GA  ++++D F  T+++VIAD PV+ V+ T LGDM+ FPK  +VN V++YVKKMVP Y++    RF +AL+ GA       D++HDD+AFLQYTGGTTGVAKGAMLTH N+VAN+ QA  W+S SG+    ++ ++TALPLYHIFALTAN L F +LG    LITNPRDM GF+KELRNNRFT  TGVNTLF GLLNTPGF+D+ F+  KV LGGGMAVQRAVAERW+ +TG  L+EAYGLTETSPAA INP+++  YNG+IGLPI STD CV+D++G  +A GE GELCIRGPQVMKGYW RPD TA+ +D DGWL TGD+ARMDEKGF YIVDR KDM+LVSGFNVYPNE+EDV A    +L V  +GVP+E+SGE VK++ VR D +++E+ + +F R +LTGYK PR V+F  ELP TNVGKILRRELRD
Sbjct:   47 FGKVLTYGQADALVTQFASYLLGELQLKKGDRVAVMMPNCLQYPIATFGILRAGLTVVNVNPLYTARELKHQLVDAGASVLVVVDNFGDTVQQVIADTPVKQVVTTGLGDMLGFPKGAIVNFVLKYVKKMVPDYRIHGAVRFREALRLGARHALPPVDVSHDDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQASAWISASGVKP-GEEVIITALPLYHIFALTANGLVFMKLGGLNYLITNPRDMKGFVKELRNNRFTAITGVNTLFNGLLNTPGFEDVDFSALKVTLGGGMAVQRAVAERWKKITGTTLVEAYGLTETSPAACINPLDLAEYNGAIGLPIPSTDACVKDDDGNMVATGEVGELCIRGPQVMKGYWHRPDETAKAMDADGWLHTGDMARMDEKGFFYIVDRKKDMILVSGFNVYPNEVEDVIAMMPGVLEVAAVGVPNEKSGEVVKVVIVRKDPNLTEEQVKEFARANLTGYKHPRIVEFRKELPKTNVGKILRRELRD 555          
BLAST of long-chain fatty acid-- ligase vs. nr
Match: gi|1064135682|gb|ODU34842.1| (long-chain-fatty-acid--CoA ligase [Xanthomonadaceae bacterium SCN 69-320] >gi|1064224462|gb|ODV19735.1| long-chain-fatty-acid--CoA ligase [Xanthomonadaceae bacterium SCN 69-25] >gi|1113636300|gb|OJY95094.1| long-chain-fatty-acid--CoA ligase [Xanthomonadales bacterium 69-70])

HSP 1 Score: 677.937 bits (1748), Expect = 0.000e+0
Identity = 319/510 (62.55%), Postives = 400/510 (78.43%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
            MG  L++ +VD+ +  FA++L   +GL +GDR+A+MMPN  QYPIA+ G LRAG+ +VNTNP+YTP EL+HQL DSGA AI+++D FAAT+ EV+AD PV+HV+ T LGD++ FPK L+ N VV+YVKK+VP +++    RFNDAL  GA     + ++ HDD+AFLQYTGGTTGVAKGAMLTHGN+VAN+ QA  W+  +  +    + ++TALPLYHIFALTAN L F + G    LITNPRDM GF+KEL   RFT  TGVNTLF GLLNTPGF  + F+   + LGGGMAVQRAVAERW+ VTG  L+EAYGLTETSPAA +NP+N+  YNGSIGLPI ST++ ++D+NG  L IG+ GELC+RGPQVMKGYW+RP+ TA+VL  +GWL TGD+ARMDEKG+V+IVDR KDM+LVSGFNVYPNEIEDV A+H  +L V  IGVPDE+SGEAVK+I VR D +++ +DL   C+ +LTGYK PRY++F D LP +NVGKILRRELRD
Sbjct:   47 MGKVLSYDDVDRLSRQFAAFLTGDLGLQRGDRVALMMPNVLQYPIAIFGVLRAGLTVVNTNPMYTPRELRHQLVDSGAAAIVVLDNFAATVAEVLADTPVKHVITTGLGDLLGFPKALITNLVVKYVKKLVPPFRIDGQIRFNDALARGARQTFHRTELGHDDIAFLQYTGGTTGVAKGAMLTHGNLVANMQQASIWIGRN--AKLGQEIIITALPLYHIFALTANGLVFMKFGGLNYLITNPRDMPGFVKELSQVRFTAITGVNTLFNGLLNTPGFDQVDFSSLHLTLGGGMAVQRAVAERWKKVTGVTLVEAYGLTETSPAACMNPLNLSEYNGSIGLPIPSTEVSIQDDNGNELPIGDIGELCVRGPQVMKGYWKRPEETAKVLTDNGWLHTGDIARMDEKGYVFIVDRKKDMILVSGFNVYPNEIEDVVATHPGVLEVAAIGVPDEKSGEAVKIIVVRKDPNLTAEDLRQHCKANLTGYKQPRYIEFRDSLPKSNVGKILRRELRD 554          
BLAST of long-chain fatty acid-- ligase vs. nr
Match: gi|655561819|ref|WP_028915634.1| (long-chain-fatty-acid--CoA ligase FadD [Pseudoxanthomonas sp. J31])

HSP 1 Score: 677.937 bits (1748), Expect = 0.000e+0
Identity = 320/510 (62.75%), Postives = 399/510 (78.24%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
             G  LT+ EVD+ A  FA+YL   +GL KGDR+A+MMPNC QYPIA  G LRAG+ +VN NPLYT  ELKHQL D+GA AI+++D F  T++EV+A+ P++ V+ T LGDM+ FPK  +VN V++YVKKMVP Y +P   RF DAL AG      + DI   D+AFLQYTGGTTGVAKGAMLTH N+VAN+ QA  WL  + +    ++ ++TALPLYHIFALTAN L F  LG    LITNPRDM  F+KEL+ + FT  TGVNTLF GLLNTPGF ++ F+  K+ LGGGMAVQRAVAERW+ VTG  L+EAYGLTETSPAA INP++++ +NGSIGLPI STD+C++DE+G+ +A+GE GELCI+GPQVMKGYWQRP+ TA+ +D DGWL TGD+AR+DEKGFVYIVDR KDM+LVSGFNVYPNEIEDV A+   +L V  +GVPDERSGEAVKL  V+ D +++ +D+  +CR++LTGYK PR+++F DELP +NVGKILR+ELRD
Sbjct:   47 FGKTLTYAEVDRLATQFAAYLLGELGLKKGDRVALMMPNCLQYPIATFGILRAGLTVVNVNPLYTARELKHQLVDAGAAAIVVVDNFGHTVQEVLAETPLKQVITTGLGDMLGFPKGAIVNFVLKYVKKMVPEYDIPGAVRFRDALAAGKRHALPQVDINPSDIAFLQYTGGTTGVAKGAMLTHRNLVANMCQASAWLRPNDLRP-GEEVIITALPLYHIFALTANCLVFMELGGLNHLITNPRDMPNFVKELKTSGFTAITGVNTLFNGLLNTPGFDEVDFSKLKITLGGGMAVQRAVAERWKKVTGVTLVEAYGLTETSPAACINPLDLKEFNGSIGLPIPSTDVCIKDEDGKEVALGEIGELCIKGPQVMKGYWQRPEETAKAIDADGWLHTGDMARIDEKGFVYIVDRKKDMILVSGFNVYPNEIEDVVAAMPGVLEVAAVGVPDERSGEAVKLFVVKKDPALTAEDIKAYCRQALTGYKQPRHIEFRDELPKSNVGKILRKELRD 555          
BLAST of long-chain fatty acid-- ligase vs. nr
Match: gi|518228582|ref|WP_019398790.1| (long-chain fatty acid--CoA ligase [Pseudoxanthomonas sp. GW2])

HSP 1 Score: 677.552 bits (1747), Expect = 0.000e+0
Identity = 320/510 (62.75%), Postives = 399/510 (78.24%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
             G  LT+ EVD+ A  FA+YL   +GL KGDR+A+MMPNC QYPIA  G LRAG+ +VN NPLYT  ELKHQL D+GA AI+++D F  T++EV+A+ P++ V+ T LGDM+ FPK  +VN V++YVKKMVP Y +P   RF DAL AG      + DI   D+AFLQYTGGTTGVAKGAMLTH N+VAN+ QA  WL  + +    ++ ++TALPLYHIFALTAN L F  LG    LITNPRDM  F+KEL+ + FT  TGVNTLF GLLNTPGF ++ F+  K+ LGGGMAVQRAVAERW+ VTG  L+EAYGLTETSPAA INP++++ +NGSIGLPI STD+C++DE+G+ +A+GE GELCI+GPQVMKGYWQRP+ TA+ +D DGWL TGD+AR+DEKGFVYIVDR KDM+LVSGFNVYPNEIEDV A+   +L V  +GVPDERSGEAVKL  V+ D +++ +D+  +CR++LTGYK PR+++F DELP +NVGKILR+ELRD
Sbjct:   47 FGKTLTYAEVDRLATQFAAYLLGELGLKKGDRVALMMPNCLQYPIATFGILRAGLTVVNVNPLYTARELKHQLVDAGAAAIVVVDNFGHTVQEVLAETPLKQVITTGLGDMLGFPKGAIVNFVLKYVKKMVPEYDIPGAVRFRDALAAGKRHALPQVDINPSDIAFLQYTGGTTGVAKGAMLTHRNLVANMCQASAWLRPNDLRP-GEEVIITALPLYHIFALTANCLVFMELGGLNHLITNPRDMPNFVKELKTSGFTAITGVNTLFNGLLNTPGFDEVDFSKLKITLGGGMAVQRAVAERWKKVTGVTLVEAYGLTETSPAACINPLDLKEFNGSIGLPIPSTDVCIKDEDGKEVALGEIGELCIKGPQVMKGYWQRPEETAKAIDADGWLRTGDMARIDEKGFVYIVDRKKDMILVSGFNVYPNEIEDVVAAMPGVLEVAAVGVPDERSGEAVKLFVVKKDPALTAEDIKAYCRQALTGYKQPRHIEFRDELPKSNVGKILRKELRD 555          
BLAST of long-chain fatty acid-- ligase vs. nr
Match: gi|1351068274|ref|WP_104604436.1| (long-chain fatty acid--CoA ligase [Xanthomonas cucurbitae] >gi|1348844959|gb|PPU75317.1| long-chain-fatty-acid--CoA ligase [Xanthomonas cucurbitae])

HSP 1 Score: 677.167 bits (1746), Expect = 0.000e+0
Identity = 317/510 (62.16%), Postives = 397/510 (77.84%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
             G  +T+ EVD+  + FA YL   + L KGDR+A+MMPNC QYPIA  G LRAG+ +VN NPLYTP ELKHQL DSGA  ++++D F  T+++VIAD PV+ V+ T LGDM+ FPK  LVN VV+Y+KK+VP Y+L    RF +AL  G+        +  DD+AFLQYTGGTTGVAKGAMLTH N+VAN+ QA +W++ +G   E ++ V+TALPLYHIFALTAN L F ++G    LI+NPRDM GF+KEL+  RFT FTGVNTLF GLLNTPGF  + F+  K+ LGGGMAVQR+VAERW++VTG+ L+EAYGLTETSPAA INPM+++ YNGSIGLPI STD C++D+ G TL IGE GELC++GPQVMKGYW++P+ TA+V+D +GWL TGD+ARMDE+GFVYIVDR KDM+LVSGFNVYPNEIEDV A+   +L V  +GVPDE+SGE VK++ V+ D +++ +D+   CR +LTGYK PR V+F  ELP TNVGKILRRELRD
Sbjct:   47 FGKTITYREVDRLVDQFAGYLLGQLQLKKGDRVALMMPNCLQYPIATFGILRAGLTVVNVNPLYTPRELKHQLVDSGASVLVVIDNFGTTVQQVIADTPVKQVITTGLGDMLGFPKGALVNFVVKYIKKLVPDYRLDGAIRFREALALGSKHNVPAVQVESDDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQAHQWIAGTGKMEEGNEVVITALPLYHIFALTANGLVFMKVGGCNHLISNPRDMPGFVKELKKTRFTAFTGVNTLFNGLLNTPGFDQIDFSSLKMTLGGGMAVQRSVAERWKNVTGQTLVEAYGLTETSPAACINPMDLKDYNGSIGLPIPSTDACIKDDAGNTLPIGEIGELCLKGPQVMKGYWKKPEETAKVMDAEGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVVATLPGVLEVAAVGVPDEKSGEIVKVVIVKKDPALTAEDVKAHCRSNLTGYKQPRVVEFRKELPKTNVGKILRRELRD 556          
BLAST of long-chain fatty acid-- ligase vs. nr
Match: gi|947723672|ref|WP_056385932.1| (long-chain fatty acid--CoA ligase [Rhodanobacter sp. Soil772] >gi|946436985|gb|KRE87314.1| long-chain fatty acid--CoA ligase [Rhodanobacter sp. Soil772])

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 318/510 (62.35%), Postives = 396/510 (77.65%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
             G +L++G++D+ +  FA YL  V+ L KGDR+A+MMPN  QYPIA+ GALRAG+V+VNTNP+YT  ELKHQL+DSGA+AI+++D FA TL+ V+A+  V+HV+ T +GD++ FPK  L+N V+++VKKMVPAY LP+  RF DAL  GA        + HDD+AFLQYTGGTTGVAKGAMLTHGNMVAN+LQA  W+ T  +    ++ ++TALPLYHIF+LTAN L F RLG    LITNPRDM GF+KEL+ + FT FTGVNTLF GLLNTPGF +L F+   ++LGGGMAVQRAVAERW+ VTG  L EAYGLTETSPA  INP++++ YNGSIGLP+ ST++ +  E+GQ L +GE GEL + GPQVMKGYW RPD TA+VL  DGWL TGD+A+MDEKG+ YIVDR KDM+LVSGFNVYPNE+ED    H  +L V  +GVPDE SGE VKL  VR D +++E+ L  FCRE+LTGYK P+ ++F D LP +NVGKILRRELRD
Sbjct:   47 FGRQLSYGQIDEMSRQFAGYLTGVLKLGKGDRIAIMMPNVLQYPIALFGALRAGLVVVNTNPMYTARELKHQLEDSGAKAIVVLDNFAGTLQHVVAETEVQHVITTGIGDLLGFPKGALINFVLKHVKKMVPAYDLPQAVRFRDALARGAAHPLQPVTLTHDDIAFLQYTGGTTGVAKGAMLTHGNMVANMLQAAAWIGTD-LVKPGEEVIITALPLYHIFSLTANGLVFMRLGGLNWLITNPRDMPGFVKELKKSGFTAFTGVNTLFNGLLNTPGFAELDFSRLHLSLGGGMAVQRAVAERWKKVTGCTLAEAYGLTETSPAVCINPLDLKEYNGSIGLPVPSTNVAIWSEDGQPLPVGEVGELMVHGPQVMKGYWNRPDETAKVLGADGWLHTGDIAKMDEKGYFYIVDRKKDMILVSGFNVYPNEVEDAVMEHPGVLEVAAVGVPDEHSGEVVKLFVVRKDPNLTEEALKQFCRENLTGYKRPKLIEFRDSLPKSNVGKILRRELRD 555          
BLAST of long-chain fatty acid-- ligase vs. nr
Match: gi|1350909666|ref|WP_104540886.1| (long-chain fatty acid--CoA ligase [Xanthomonas codiaei] >gi|1348833079|gb|PPU64062.1| long-chain-fatty-acid--CoA ligase [Xanthomonas codiaei])

HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0
Identity = 320/510 (62.75%), Postives = 391/510 (76.67%), Query Frame = 0
Query:    1 MGNKLTFGEVDQYANAFASYLRHVIGLNKGDRMAMMMPNCSQYPIAVLGALRAGVVIVNTNPLYTPTELKHQLKDSGARAILIMDLFAATLEEVIADHPVEHVLLTSLGDMMPFPKRLLVNAVVRYVKKMVPAYQLPKVTRFNDALKAGATAKAVKEDIAHDDLAFLQYTGGTTGVAKGAMLTHGNMVANVLQAEEWLSTSGISSENDDCVVTALPLYHIFALTANLLTFTRLGVRTLLITNPRDMLGFIKELRNNRFTIFTGVNTLFAGLLNTPGFKDLPFADFKVALGGGMAVQRAVAERWQDVTGKPLLEAYGLTETSPAASINPMNIEGYNGSIGLPISSTDMCVRDENGQTLAIGETGELCIRGPQVMKGYWQRPDATAEVLDQDGWLSTGDVARMDEKGFVYIVDRLKDMVLVSGFNVYPNEIEDVAASHEKILSVGVIGVPDERSGEAVKLIAVRSDDSVSEQDLIDFCRESLTGYKIPRYVQFVDELPMTNVGKILRRELRD 510
             G  +T+ E DQ    FA+YL   + L KGDR+A+MMPNC QYPIA  G LRAG+ +VN NPLYTP ELKHQL DSGA  ++++D F  T+++VIA+ PV+ V+ T LGDM+ FPK  L+N VV+YVKK+VP Y++    RF +AL  G         I  DD+AFLQYTGGTTGVAKGAMLTH N+VAN+ QA +WLS +G   E  + V+TALPLYHIFALTAN L F ++G    LI+NPRDM GF+KEL+  RFT FTGVNTLF GLLNTPGF  + F+  K+ LGGGMAVQR+VAERW+ VTG+ L+EAYGLTETSPAA INPM+++ YNGSIGLPI STD C++D+ G TL IGE GELCI+GPQVMKGYW++PD TA+V+D DGWL TGD+ARMDE+GFVYIVDR KDM+LVSGFNVYPNEIEDV A+   +L V  +GVPDE+SGE VK + V+ D +++  D+   CR +LTGYK PR ++F  ELP TNVGKILRRELRD
Sbjct:   47 FGKTITYREADQLVEQFAAYLLGELQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVIDNFGTTVQQVIAETPVKQVITTGLGDMLGFPKGTLINFVVKYVKKLVPDYRIDGAIRFREALALGRKHSVPTLQIEADDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQAHQWLSGTGKMEEGKEVVITALPLYHIFALTANGLVFMKIGGCNHLISNPRDMPGFVKELKKTRFTAFTGVNTLFNGLLNTPGFDQIDFSSLKMTLGGGMAVQRSVAERWKKVTGQTLVEAYGLTETSPAACINPMDLKDYNGSIGLPIPSTDACIKDDAGATLPIGEIGELCIKGPQVMKGYWKKPDETAKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIATLPGVLEVAAVGVPDEKSGELVKAVIVKKDPALTADDVKAHCRANLTGYKQPRVIEFRKELPKTNVGKILRRELRD 556          
The following BLAST results are available for this feature:
BLAST of long-chain fatty acid-- ligase vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides)
Total hits: 18
Match NameE-valueIdentityDescription
EMLSAG000000017095.109e-7732.82supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1... [more]
EMLSAG000000063096.186e-3727.46supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-... [more]
EMLSAG000000099042.696e-3628.61supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-... [more]
EMLSAG000000070526.595e-2325.21supercontig:LSalAtl2s:LSalAtl2s3:1758018:1760492:-... [more]
EMLSAG000000070513.598e-1923.87supercontig:LSalAtl2s:LSalAtl2s3:1688664:1690882:-... [more]
EMLSAG000000020812.252e-1821.00supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1... [more]
EMLSAG000000069464.953e-1821.85supercontig:LSalAtl2s:LSalAtl2s393:601207:603901:-... [more]
EMLSAG000000049346.470e-1821.72supercontig:LSalAtl2s:LSalAtl2s259:871050:904452:1... [more]
EMLSAG000000128919.468e-1823.60supercontig:LSalAtl2s:LSalAtl2s981:104426:107346:-... [more]
EMLSAG000000104016.204e-1728.17supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 ... [more]

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BLAST of long-chain fatty acid-- ligase vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
gi|24637994|sp|Q8ZES9.1|LCFA_YERPE0.000e+057.84RecName: Full=Long-chain-fatty-acid--CoA ligase; A... [more]
gi|24637992|sp|Q8XDR6.1|LCFA_ECO570.000e+056.56RecName: Full=Long-chain-fatty-acid--CoA ligase; A... [more]
gi|54037718|sp|P63522.1|LCFA_SALTI0.000e+056.56RecName: Full=Long-chain-fatty-acid--CoA ligase; A... [more]
gi|60392603|sp|P69451.1|LCFA_ECOLI0.000e+056.16RecName: Full=Long-chain-fatty-acid--CoA ligase; A... [more]
gi|1170729|sp|P46450.1|LCFA_HAEIN0.000e+053.82RecName: Full=Long-chain-fatty-acid--CoA ligase; A... [more]
gi|2829810|sp|P94547.1|LCFA_BACSU4.215e-11439.33RecName: Full=Long-chain-fatty-acid--CoA ligase; A... [more]
gi|239938656|sp|O07610.2|LCFB_BACSU4.298e-10237.40RecName: Full=Long-chain-fatty-acid--CoA ligase; A... [more]
gi|75075869|sp|Q4R4Z9.1|ACSF2_MACFA1.257e-6931.86RecName: Full=Acyl-CoA synthetase family member 2,... [more]
gi|123781622|sp|Q499N5.1|ACSF2_RAT1.774e-6931.93RecName: Full=Acyl-CoA synthetase family member 2,... [more]
gi|81669033|sp|O31826.1|YNGI_BACSU2.449e-6931.49RecName: Full=Putative acyl-CoA synthetase YngI[more]

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BLAST of long-chain fatty acid-- ligase vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR)
Total hits: 25
Match NameE-valueIdentityDescription
gi|906867123|ref|WP_049725553.1|0.000e+065.38long-chain fatty acid--CoA ligase [Wenzhouxiangell... [more]
gi|1148783658|ref|WP_077513564.1|0.000e+063.14long-chain-fatty-acid--CoA ligase [Rhodanobacter s... [more]
gi|947300907|ref|WP_056007295.1|0.000e+063.53long-chain fatty acid--CoA ligase [Frateuria sp. S... [more]
gi|946850140|ref|WP_055773187.1|0.000e+063.53MULTISPECIES: long-chain fatty acid--CoA ligase [S... [more]
gi|1064135682|gb|ODU34842.1|0.000e+062.55long-chain-fatty-acid--CoA ligase [Xanthomonadacea... [more]
gi|655561819|ref|WP_028915634.1|0.000e+062.75long-chain-fatty-acid--CoA ligase FadD [Pseudoxant... [more]
gi|518228582|ref|WP_019398790.1|0.000e+062.75long-chain fatty acid--CoA ligase [Pseudoxanthomon... [more]
gi|1351068274|ref|WP_104604436.1|0.000e+062.16long-chain fatty acid--CoA ligase [Xanthomonas cuc... [more]
gi|947723672|ref|WP_056385932.1|0.000e+062.35long-chain fatty acid--CoA ligase [Rhodanobacter s... [more]
gi|1350909666|ref|WP_104540886.1|0.000e+062.75long-chain fatty acid--CoA ligase [Xanthomonas cod... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
scaffold4073_size6756supercontigscaffold4073_size6756:2545..4098 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
maker2018-02-12 .496401
T. kinsejongensis vs L. Salmonis peptides2018-04-19
T. kingejongensis peptided Blastp vs. SwissProt2018-04-19
T. kingsejongensis proteins Blastp vs. NR2018-05-15
Properties
Property NameValue
Note-
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
snap_masked-scaffold4073_size6756-processed-gene-0.2-mRNA-1snap_masked-scaffold4073_size6756-processed-gene-0.2-mRNA-1Tigriopus kingsejongensismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at scaffold4073_size6756:2545..4098-

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>snap_masked-scaffold4073_size6756-processed-gene-0.2 ID=snap_masked-scaffold4073_size6756-processed-gene-0.2|Name=long-chain fatty acid-- ligase|organism=Tigriopus kingsejongensis|type=gene|length=1554bp|location=Sequence derived from alignment at scaffold4073_size6756:2545..4098- (Tigriopus kingsejongensis)
ATGGGCAACAAGTTGACCTTTGGCGAAGTGGATCAGTACGCCAATGCCTT TGCGTCCTATCTTCGTCATGTGATTGGCCTGAACAAGGGCGATCGCATGG CCATGATGATGCCCAACTGCAGCCAGTACCCGATCGCGGTATTGGGGGCC TTGCGGGCAGGGGTGGTGATCGTCAACACCAATCCGCTCTATACGCCCAC CGAGCTCAAACACCAGCTGAAAGACTCGGGTGCGCGGGCTATTTTGATCA TGGACTTGTTTGCTGCAACCCTTGAAGAAGTGATTGCAGATCACCCCGTC GAGCATGTTTTGTTGACTTCGCTGGGTGACATGATGCCGTTTCCTAAGCG TCTGCTGGTCAATGCGGTAGTGCGCTATGTGAAGAAAATGGTGCCAGCTT ATCAGTTGCCAAAAGTGACGCGTTTTAATGATGCGCTAAAAGCCGGCGCT ACTGCAAAAGCAGTCAAAGAGGACATTGCACACGATGACCTCGCTTTTCT GCAATACACGGGTGGCACGACCGGTGTGGCTAAAGGCGCGATGCTGACCC ACGGCAATATGGTGGCCAATGTATTACAAGCCGAAGAATGGCTGTCTACT TCTGGTATCTCCTCAGAAAACGACGATTGTGTTGTAACGGCTCTGCCGCT CTACCATATCTTCGCTTTAACAGCGAACCTATTAACCTTCACGCGGCTAG GCGTTCGCACGTTGCTGATCACAAACCCGCGTGATATGCTCGGCTTTATT AAAGAGCTCCGAAACAACCGCTTCACCATTTTTACCGGTGTGAACACTTT GTTCGCAGGCTTGTTAAACACGCCCGGATTCAAAGACCTGCCTTTTGCTG ATTTTAAAGTTGCGCTTGGTGGTGGTATGGCGGTGCAACGTGCGGTGGCA GAGCGCTGGCAAGACGTGACCGGCAAGCCATTGCTCGAGGCCTATGGGCT GACAGAGACTTCGCCTGCCGCCTCTATTAACCCGATGAACATTGAGGGTT ATAACGGTTCTATCGGCCTGCCTATTTCATCTACTGATATGTGCGTGCGA GACGAAAACGGCCAAACACTGGCTATCGGTGAGACCGGTGAGCTGTGTAT TCGTGGACCACAGGTCATGAAGGGCTACTGGCAGCGGCCTGACGCCACAG CTGAAGTACTCGATCAAGACGGTTGGCTGAGCACGGGGGATGTTGCCCGT ATGGACGAAAAGGGCTTTGTCTATATTGTCGATCGCTTGAAAGATATGGT GCTGGTGTCCGGTTTTAATGTGTATCCCAACGAAATAGAAGACGTGGCCG CCAGCCACGAAAAAATATTGTCCGTTGGTGTCATCGGCGTGCCGGATGAG CGCTCGGGTGAAGCCGTCAAGTTGATCGCTGTGCGCAGTGACGACAGTGT ATCTGAGCAGGATTTGATCGACTTTTGTCGTGAGAGTTTGACCGGCTACA AAATCCCGCGCTATGTGCAATTTGTCGACGAGCTGCCCATGACAAACGTC GGTAAAATCTTGCGTCGCGAATTGCGCGATCTCTACGGTGGGCAGCAATC GTGA
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Synonyms
The feature 'long-chain fatty acid-- ligase' has the following synonyms
Synonym
Tk10916
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