BLAST of predicted protein vs. L. salmonis genes
Match: EMLSAG00000001163 (supercontig:LSalAtl2s:LSalAtl2s1193:681:9273:1 gene:EMLSAG00000001163 transcript:EMLSAT00000001163 description:"maker-LSalAtl2s1193-augustus-gene-0.21")

HSP 1 Score: 502.671 bits (1293), Expect = 7.462e-172
Identity = 259/634 (40.85%), Postives = 389/634 (61.36%), Query Frame = 0

Query:   18 GWFFSNPEVISAIKVLVVLATGFLLVQVIRWSRMAEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAG-RRVLVLEQHD-QAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFF-ALLDE--TSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQ-FRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMS---RDLPMIFISFPSTKDPRWNERPGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQEAKAKSD 642
             W FS P +  +IK+ + L++ FL VQ+ RW + A+ +NP +  + +K+     E +++ R     F P+++P   +AI IGSG   L  A +++R+G + +++LE HD + GGS H + E G+EFDVGIHYIG+M + ++ ++LV+ ++E  LEWE +    D V          +PV+   + W   L   FPKE  GI+ FF   LD+      +     LK  PL +   +I   ++ +F  LW  + +R+S  ++++ LT DKDLQ ++ Y W DYG+ P+ + + MQ+++ +H+ EG  YPVGG+S IA  +IP IE++GG+    APV  I++NG K MGVRV +G   Y I+AP+++S AG+ NTF  LLP  V+  SY+ E+L      PG++ MS+F+G + + E+L++ K N+WA+   E       +L   V+DI++     +P++FISFPSTKD    E    + KS+C +I  ANW W+ +++    KKRGDEY  LK  +G   ID+   ++P L+HHI YV +G+PVTN+ Y+GS  GEIYGLDH   R  P   A LRP TDIPGLYL+GQDV+TCGF GA F G+  A +VL RN+M DL+  H++  +E  AK D
Sbjct:    5 AWLFSVPILKLSIKIAIALSSLFLFVQIARWRKQAQYKNPFESKISRKKP---GENKKKRR-----FDPNELPPYPEAIFIGSGTSSLMCANIMSRSGYKNIVILESHDTEVGGSTHVFNESGFEFDVGIHYIGEMTKRSLAKSLVDTLTENNLEWEDMGDPFDCVY----TSKELHPVHVGLQNWKEYLLKKFPKESDGIETFFDKYLDKHTCKKFSTTQFILKSIPLLIANFLIKFRILELFG-LWNDKIYRTSVKDLVESLTQDKDLQKLMCYSWADYGTPPNLAPWAMQALIFKHFGEGGAYPVGGSSRIAECLIPPIEEAGGKXFCNAPVSEILYNGSKVMGVRVMRGSSSYDIKAPLVVSGAGIRNTFNYLLPPTVSSLSYYKEMLNHELKDPGMSMMSIFLGFDKAQEELQINKSNIWAHFG-ESLNSVTEFLGLRVEDIVNSKENQVPLLFISFPSTKDSSRLENEDLKYKSSCTLIGPANWEWFSKWDKYEVKKRGDEYNTLKSQLGQKYIDRLLEIFPSLQHHISYVRMGSPVTNKTYLGSPFGEIYGLDHTIERFHPNNIAKLRPKTDIPGLYLTGQDVLTCGFVGAAFSGVLTANSVLDRNIMNDLLRHHQRSAREKDAKKD 624          

BLAST of predicted protein vs. SwissProt
Match: gi|82230779|sp|Q5BLE8.1|RETST_DANRE (RecName: Full=Putative all-trans-retinol 13,14-reductase; AltName: Full=All-trans-13,14-dihydroretinol saturase; Short=RetSat; Flags: Precursor)

HSP 1 Score: 488.034 bits (1255), Expect = 7.709e-165
Identity = 249/605 (41.16%), Postives = 373/605 (61.65%), Query Frame = 0

Query:   33 LVVLATGFLLVQVIRWSRMAEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGHK-AMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQ 635
              V+A    LV  +    +    NP   D R+  +P + + + + +++++ F   +VP DLDA+V+GSGIGGL  A LLA+ G++VLVLEQHD+AGG CHT+ E+G+EFDVGIHYIG++     LR +++Q++ GQL+W+PLE   D V IG   + R Y +Y  R+R++  LK  FP E + ID +  L  E      V+  LK  P  +   ++ TGL +  T   R   RS T +++ ELT +KDL+ VLSY +  YG  P  + F M S++  HYM GA+YP GGA+EIA ++IP+IEK+GG VLVRAPV  I+ N  K A+GV V KG E   + AP++IS AG+ NT+  LLPK V       + L+   ++ G + +S+F+GL+ + E+L LK +N + Y  N  ++  + Y   + ++   ++ P+IF++ PS KD  W ER PG   KST  ++SFAN+ W++++++   K R  +Y++LK+   N +++    +YP++K  I+YV+ GTP+TNQ YI + +GEIYG DH   R     NA +R  T I  LYL+GQD+M CGF GAL G L C   +L RN+  +   L K+++ 
Sbjct:    3 FAVVAIFLALVAFLYRYVVGSGPNPFAIDTREPLKPMVFDRKLKNKVLKQGFLASRVPEDLDAVVVGSGIGGLAIAVLLAKVGKKVLVLEQHDRAGGCCHTFKEQGFEFDVGIHYIGELSNHKPLRCIIDQMTNGQLQWDPLENPFDNVVIGPPENRRIYQIYSGRKRYMDELKKCFPGEEKAIDEYVRLCKEVGQGVWVMVLLKFLPTPIANFLVRTGLANRLTSFSRYASRSLT-DVVNELTQNKDLRAVLSYIFGTYGKIPKEASFSMHSLIVNHYMNGAWYPKGGATEIAYHMIPIIEKAGGAVLVRAPVNRILLNDAKEAIGVSVLKGQEEVHVRAPIVISDAGIFNTYEYLLPKDVQTMPAIQKQLS--MLQHGDSGLSIFIGLDGTKEELGLKADNYFIYPENNIDELLEDYRSGNREESAKKN-PLIFVASPSAKDSTWPERTPG---KSTLTVVSFANYEWFEEWKDDKVKNRSTDYKQLKELFINYILEAVTEIYPKIKDRIEYVDAGTPITNQHYIAAPRGEIYGADHGIPRFSAELNATIRAQTPIKNLYLTGQDLMLCGFAGALTGALTCGSVILNRNLHLEAFSLAKRVQN 600          

BLAST of predicted protein vs. SwissProt
Match: gi|341942157|sp|Q64FW2.3|RETST_MOUSE (RecName: Full=All-trans-retinol 13,14-reductase; AltName: Full=All-trans-13,14-dihydroretinol saturase; Short=RetSat; AltName: Full=PPAR-alpha-regulated and starvation-induced gene protein; Flags: Precursor)

HSP 1 Score: 463.766 bits (1192), Expect = 2.752e-155
Identity = 237/589 (40.24%), Postives = 364/589 (61.80%), Query Frame = 0

Query:   52 AEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSI-VHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQEAK 638
            A + NP   D+++   P +T+   R ++++++F   +VP  LDA+VIGSGIGGL +AA+LA+AG+RVLVLEQH +AGG CHT+ E G EFD GIHYIG+M    I R +++Q++EGQL+W P+    D++ +      +++P+Y  R+ +I  LK  FPKE   ID +  L+   +   +    LK  PL L +L+   GL+  F+   R   +S   E++Q+L + ++LQ VLSY +P YG  PS + F + ++L  HY++GA+YP GG+SEIA + IP+I+++GG VL RA VQS+ + +  +A GV V+KG E+  I  PV+IS AG+ NT++ LLP+ V       + LA   +RPG++ +S+F+ L  + EDLKL+  N + Y   + +K  + Y+ S  K+     +P++FI+FPS+KDP W ER P R   +  A++  A   + +  +     KRG +YE LK+      +    +L+PQL+  ++ V  G+P+TNQ+Y+ + +G  YG DHD  R  P   A +R  T IP LYL+GQD+ TCG  GAL G L C+ A+L RN+  DL  L  +++ + K
Sbjct:   26 ASSPNPFAEDVKRPPEPLVTDKEARKKVLKQAFSVSRVPEKLDAVVIGSGIGGLASAAVLAKAGKRVLVLEQHTKAGGCCHTFGENGLEFDTGIHYIGRMREGNIGRFILDQITEGQLDWAPMASPFDLMILEGPNGRKEFPMYSGRKEYIQGLKKKFPKEEAVIDKYMELVKVVARGVSHAVLLKFLPLPLTQLLSKFGLLTRFSPFCRASTQSLA-EVLQQLGASRELQAVLSYIFPTYGVTPSHTAFSLHALLVDHYIQGAYYPRGGSSEIAFHTIPLIQRAGGAVLTRATVQSVLLDSAGRACGVSVKKGQELVNIYCPVVISNAGMFNTYQHLLPETVRHLPDVKKQLA--MVRPGLSMLSIFICLKGTKEDLKLQSTNYYVYFDTDMDKAMERYV-SMPKEKAPEHIPLLFIAFPSSKDPTWEERFPDRSTMT--ALVPMAFEWFEEW-QEEPKGKRGVDYETLKNAFVEASMSVIMKLFPQLEGKVESVTGGSPLTNQYYLAAPRGATYGADHDLARLHPHAMASIRAQTPIPNLYLTGQDIFTCGLMGALQGALLCSSAILKRNLYSDLQALGSKVKAQKK 607          

BLAST of predicted protein vs. SwissProt
Match: gi|81871478|sp|Q8VHE9.1|RETST_RAT (RecName: Full=All-trans-retinol 13,14-reductase; AltName: Full=All-trans-13,14-dihydroretinol saturase; Short=RetSat; AltName: Full=PPAR-alpha-regulated and starvation-induced gene protein; AltName: Full=RMT-7; Flags: Precursor)

HSP 1 Score: 456.062 bits (1172), Expect = 2.963e-152
Identity = 233/586 (39.76%), Postives = 364/586 (62.12%), Query Frame = 0

Query:   56 NPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSI-VHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNERPGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQEAKAK 640
            NP   D+++   P +T+   R ++++++F   +VP  LDA+VIGSGIGGL +AA+LA+AG+RVLVLEQH +AGG CHT+ E G EFD GIHYIG+M    I R +++Q++EGQL+W P+    D++ +      +++P+Y  R+ +I  LK  FPKE   ID +  L+   ++  +    LK  PL L +L+   GL+  F+   R   +S   E++++L +  +LQ VLSY  P YG  PS + F + ++L  HY++GA+YP  G+SEIA + IP+I+++GG VL RA VQS+ + +  +A GV V+KG E+  I  PV+IS AG+ NT++ LLP++V       + L    ++PG++ +S+F+ L  + E+LKL+  N + Y   + +K  + Y+ S  K+     +P++FI FPS+KDP W +R    ++ST  ++    + W+++++     KRG +YE LK+T     +    +L+PQL+  ++ V  G+P+TNQ+Y+ + +G  YG DHD  R  P   A LR  T IP LYL+GQD+ TCG  GAL G L C+ A+L RN+  DL  L  ++R + K K
Sbjct:   30 NPFAEDVKRPPEPLVTDKEARKKVLKQAFSVSRVPEKLDAVVIGSGIGGLASAAVLAKAGKRVLVLEQHTKAGGCCHTFGENGLEFDTGIHYIGRMREGNIGRFILDQITEGQLDWAPMASPFDLMILEGPNGRKEFPMYSGRKEYIQGLKEKFPKEEAVIDKYMELVKVVAHGVSHAILLKFLPLPLTQLLNKFGLLTRFSPFCRASTQSLA-EVLKQLGASPELQAVLSYILPTYGVTPSHTTFSLHALLVDHYIQGAYYPRRGSSEIAFHTIPLIQRAGGAVLTRATVQSVLLDSAGRACGVSVKKGQELVNIYCPVVISNAGMFNTYQHLLPESVRYLPDVKKQL--TMVKPGLSMLSIFICLKGTKEELKLQSTNYYVYFDTDMDKAMECYV-SMPKEKAPEHIPLLFIPFPSSKDPTWEDR--FPDRSTMTVLVPTAFEWFEEWQEEPKGKRGVDYETLKNTFREASMSVIMKLFPQLEGKVESVTGGSPLTNQYYLAAHRGATYGADHDLARLHPHAMASLRAQTPIPNLYLTGQDIFTCGLMGALQGALLCSSAILKRNLYSDLQALGSKVRAQKKKK 609          

BLAST of predicted protein vs. SwissProt
Match: gi|75043060|sp|Q64FG0.1|RETST_MACFA (RecName: Full=All-trans-retinol 13,14-reductase; AltName: Full=All-trans-13,14-dihydroretinol saturase; Short=RetSat; AltName: Full=PPAR-alpha-regulated and starvation-induced gene protein; Flags: Precursor)

HSP 1 Score: 440.654 bits (1132), Expect = 2.272e-146
Identity = 237/588 (40.31%), Postives = 361/588 (61.39%), Query Frame = 0

Query:   53 EARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSI-VHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQEAK 638
            ++ NP   D+++   P +T+   R ++++++F   +VP  LD +VIGSG GGL AAA+LA+AG+RVLVLEQH +AGG+CHT+ E G EFD GIHYIG+M   +I R +++Q++EGQL+W P+    D++ +      ++YP+Y   + +I  LK  FP+E   ID +  L+   SN  A    LK  PL +++L+   GL+  F+       +S   E++Q+L +  +LQ VLSY +P YG  P  S F M ++L  HY++GAFYP GG+SEIA + IPVI+++GG VL RA VQS+ + +  KA GV V+KG E+  I  PV++S AG+ NT+  LLP          + L    +RPG+  MSVF+ L  + EDL L   N + Y   + ++  + Y+ S  ++  +  +P++FI+FPS KDP W +R PGR   S+  ++  + + W+++++     KRG +YE  K++     +  A +L+PQL+  ++ V  G+P+TNQFY+ + +G  YG DHD  R  P   A LR  + IP LYL+GQD+ TCG  GAL G L C+ A+L RN+  DL  L  +I+ + K
Sbjct:   28 KSPNPFSEDVKRPPAPLVTDKEARKKVLKQAFSASRVPEKLDVVVIGSGFGGLAAAAILAKAGKRVLVLEQHTKAGGACHTFGENGLEFDTGIHYIGRMEEGSIGRFILDQITEGQLDWVPMSSPFDIMVLEGPNGRKEYPMYSGEKAYIQGLKEKFPQEEAIIDKYIKLVKVVSNGVAHAILLKFLPLPVIQLLDRCGLLTRFSPFLHASTQSLA-EVLQQLGASSELQAVLSYIFPTYGVTPRHSAFSMHALLVNHYLKGAFYPRGGSSEIAFHTIPVIQRAGGAVLTRATVQSVLLDSAGKACGVSVKKGHELVNIYCPVVVSNAGLFNTYEHLLPGNARCLPGVKQQLG--MVRPGLGMMSVFICLQGTKEDLHLPSTNYYVYHDTDMDQAMERYV-SMPREKAAEHIPLLFIAFPSAKDPTWEDRFPGR---SSMIMLIPSAYEWFEEWQAELKGKRGSDYETYKNSFVEASMSVAMKLFPQLEGKVESVTAGSPLTNQFYLAAPRGACYGADHDLGRLHPRVMASLRAQSPIPNLYLTGQDIFTCGLVGALQGALLCSSAILKRNLYSDLKDLGSRIQAQKK 608          

BLAST of predicted protein vs. SwissProt
Match: gi|90108452|sp|Q6NUM9.2|RETST_HUMAN (RecName: Full=All-trans-retinol 13,14-reductase; AltName: Full=All-trans-13,14-dihydroretinol saturase; Short=RetSat; AltName: Full=PPAR-alpha-regulated and starvation-induced gene protein; Flags: Precursor)

HSP 1 Score: 438.343 bits (1126), Expect = 1.803e-145
Identity = 234/585 (40.00%), Postives = 356/585 (60.85%), Query Frame = 0

Query:   56 NPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSI-VHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQEAK 638
            NP   D+++   P +T+   R ++++++F  +QVP  LD +VIGSG GGL AAA+LA+AG+RVLVLEQH +AGG CHT+ + G EFD GIHYIG+M   +I R +++Q++EGQL+W PL    D++ +      ++YP+Y   + +I  LK  FP+E   ID +  L+   S+       LK  PL +V+L+   GL+  F+   +   +S   E++Q+L +  +LQ VLSY +P YG  P+ S F M ++L  HYM+G FYP GG+SEIA + IPVI+++GG VL +A VQS+ + +  KA GV V+KG E+  I  P+++S AG+ NT+  LLP          + L +  +RPG+   SVF+ L  + EDL L   N + Y   + ++  + Y+ S  ++  +  +P++F +FPS KDP W +R PGR   ST  ++    + W+++++     KRG +YE  K++     +    +L+PQL+  ++ V  G+P+TNQFY+ + +G  YG DHD  R  P   A LR  + IP LYL+GQD+ TCG  GAL G L C+ A+L RN+  DL  L  +IR + K
Sbjct:   31 NPFSEDVKRPPAPLVTDKEARKKVLKQAFSANQVPEKLDVVVIGSGFGGLAAAAILAKAGKRVLVLEQHTKAGGCCHTFGKNGLEFDTGIHYIGRMEEGSIGRFILDQITEGQLDWAPLSSPFDIMVLEGPNGRKEYPMYSGEKAYIQGLKEKFPQEEAIIDKYIKLVKVVSSGAPHAILLKFLPLPVVQLLDRCGLLTRFSPFLQASTQSLA-EVLQQLGASSELQAVLSYIFPTYGVTPNHSAFSMHALLVNHYMKGGFYPRGGSSEIAFHTIPVIQRAGGAVLTKATVQSVLLDSAGKACGVSVKKGHELVNIYCPIVVSNAGLFNTYEHLLPGNARCLPGVKQQLGT--VRPGLGMTSVFICLRGTKEDLHLPSTNYYVYYDTDMDQAMERYV-SMPREEAAEHIPLLFFAFPSAKDPTWEDRFPGR---STMIMLIPTAYEWFEEWQAELKGKRGSDYETFKNSFVEASMSVVLKLFPQLEGKVESVTAGSPLTNQFYLAAPRGACYGADHDLGRLHPCVMASLRAQSPIPNLYLTGQDIFTCGLVGALQGALLCSSAILKRNLYSDLKNLDSRIRAQKK 608          

BLAST of predicted protein vs. SwissProt
Match: gi|75282667|sp|Q52QW3.1|CRTS1_ONCHC (RecName: Full=Prolycopene isomerase 1, chloroplastic; Short=CrtISO1; AltName: Full=Carotenoid isomerase 1; AltName: Full=OcrtISO22; Flags: Precursor)

HSP 1 Score: 113.235 bits (282), Expect = 4.588e-25
Identity = 157/554 (28.34%), Postives = 233/554 (42.06%), Query Frame = 0

Query:   92 DLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVG---IHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYG-ADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTL---------EIIQELTSDKDLQTVL-SYCWPDYGSKPSSSHFMMQSIL--NRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWA--YLS-NEGEKDFQTYLDSDVKDIMSRD------LPM--IFISFPSTKDPRWNERPGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQ-ACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRP--TTDIPGLYLSGQDVMTC-GFGGALFGGLFCA 614
            + DAIVIGSGIGGL AA  LA  G RVLVLE++   GGS   +   G+ FDVG   +      G   ++   +E V + +L   P     D  ++ +   DN    V+ + + +I+ L   FP E +GI  F+   +E       L +L+L  L           +++F + +R      TL         +I ++   D  L + + + C+     K   +  +  S++  +RH+  G  YPVGG   IA  +   +   G ++L +A V  I+    KA+GV++  G E +   A  +IS A   +TF KLL                                +   E+   KK  + A  +LS + G K F    D+D    +  D      LP   IF+S P+  DP      G          S  NW      E  SHK    EYEE K+ V + +I +   +L+P LK  +   EVGTP T++ ++    G  YG      R+ P    L  P  TT I GLY  G       G     F G+ CA
Sbjct:   69 EYDAIVIGSGIGGLVAATQLAVKGARVLVLEKYVIPGGSSGFFQRDGFTFDVGSSVMFGFSDKGNLNLITQALEAV-DCKLRTIP-----DPTTVHFHLPDNLSIRVHREYDMFISELVNYFPHEKEGIRRFY---NECWKIFNSLNSLELKSL--------EEPMYLFGQFFRKPVECLTLAYYLPQNAGDIARKFIKDPQLLSFIDAECFIVSTVKALHTPMINASMVLCDRHF-GGINYPVGGVGGIAKALANGLVNKGSKLLYKANVTKILLKDGKAVGVKLSNGREFF---AKTVISNATRWDTFGKLLKVE-----------------------------DIPQEEKNFKKIYLKAPSFLSIHMGVKAFVLPPDTDCHHFILEDNWGRLELPYGSIFLSIPTVLDPSLAPE-GHHIFHIFTTSSIENW------EGLSHK----EYEEKKELVADEIITRLEKKLFPGLKDSVVLKEVGTPKTHRRFLARDSG-TYG---PMPRKVP-KGLLGMPFNTTAINGLYCVGDSCFPGQGVIAVAFSGVMCA 556          

BLAST of predicted protein vs. SwissProt
Match: gi|75282666|sp|Q52QW2.1|CRTS2_ONCHC (RecName: Full=Prolycopene isomerase 2, chloroplastic; Short=CrtISO2; AltName: Full=Carotenoid isomerase 2; AltName: Full=OcrtISO24; Flags: Precursor)

HSP 1 Score: 113.235 bits (282), Expect = 5.741e-25
Identity = 157/554 (28.34%), Postives = 233/554 (42.06%), Query Frame = 0

Query:   92 DLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVG---IHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYG-ADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTL---------EIIQELTSDKDLQTVL-SYCWPDYGSKPSSSHFMMQSIL--NRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWA--YLS-NEGEKDFQTYLDSDVKDIMSRD------LPM--IFISFPSTKDPRWNERPGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQ-ACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRP--TTDIPGLYLSGQDVMTC-GFGGALFGGLFCA 614
            + DAIVIGSGIGGL AA  LA  G RVLVLE++   GGS   +   G+ FDVG   +      G   ++   +E V + +L   P     D  ++ +   DN    V+ + + +I+ L   FP E +GI  F+   +E       L +L+L  L           +++F + +R      TL         +I ++   D  L + + + C+     K   +  +  S++  +RH+  G  YPVGG   IA  +   +   G ++L +A V  I+    KA+GV++  G E +   A  +IS A   +TF KLL                                +   E+   KK  + A  +LS + G K F    D+D    +  D      LP   IF+S P+  DP      G          S  NW      E  SHK    EYEE K+ V + +I +   +L+P LK  +   EVGTP T++ ++    G  YG      R+ P    L  P  TT I GLY  G       G     F G+ CA
Sbjct:   69 EYDAIVIGSGIGGLVAATQLAVKGARVLVLEKYVIPGGSSGFFQRDGFTFDVGSSVMFGFSDKGNLNLITQALEAV-DCKLRTIP-----DPTTVHFHLPDNLSIRVHREYDMFISELVNYFPHEKEGIRRFY---NECWKIFNSLNSLELKSL--------EEPMYLFGQFFRKPVECLTLAFYLPQNAGDIARKFIKDPQLLSFIDAECFIVSTVKALHTPMINASMVLCDRHF-GGINYPVGGVGGIAKALANGLVNKGSKLLYKANVTKILLKDGKAVGVKLSNGREFF---AKTVISNATRWDTFGKLLKVE-----------------------------DIPQEEKNFKKIYLKAPSFLSIHMGVKAFVLPPDTDCHHFILEDNWGRLELPYGSIFLSIPTVLDPSLAPE-GHHIFHIFTTSSIENW------EGLSHK----EYEEKKELVADEIITRLEKKLFPGLKDSVVLKEVGTPKTHRRFLARDSG-TYG---PMPRKVP-KGLLGMPFNTTAINGLYCVGDSCFPGQGVIAVAFSGVMCA 556          

BLAST of predicted protein vs. SwissProt
Match: gi|75159637|sp|Q8S4R4.1|CRTSO_SOLLC (RecName: Full=Prolycopene isomerase, chloroplastic; Short=CrtISO; AltName: Full=Carotenoid isomerase; AltName: Full=Protein tangerine; Flags: Precursor)

HSP 1 Score: 111.309 bits (277), Expect = 2.615e-24
Identity = 146/536 (27.24%), Postives = 227/536 (42.35%), Query Frame = 0

Query:   94 DAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNR-QYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTL---------EIIQELTSDKDLQTVL-SYCWPDYGSKPSSSHFMMQSIL--NRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNERPGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACR-LYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTC-GFGGALFGGLFCA 614
            DAIVIGSGIGGL AA  LA  G +VLVLE++   GGS   Y   GY+FDVG   +        L  + + ++        LE+  D  ++ +   N     ++ + + +I  L   FP E +GI  F++   +  N+   L +L+L  L           I++F + ++      TL          I ++   D  L + + + C+         +  +  S++  +RH+  G  YPVGG  EIA ++   ++  G Q+L RA V SI+ +  KA+GV++  G + Y   A  I+S A   +TF KLL    AE     E           + +S+ +G+ A                    + D   ++  D    + +    IF+S P+  D       G          S  +W      E  S K    +YE  K+ V   +I +  + L+P LK  I + EVGTP T++ Y+    G  YG     T +  L   +   TT I GLY  G       G     F G+ CA
Sbjct:   99 DAIVIGSGIGGLVAATQLAVKGAKVLVLEKYVIPGGSSGFYERDGYKFDVGSSVMFGFSDKGNLNLITQALAA---VGRKLEVIPDPTTVHFHLPNDLSVRIHREYDDFIEELVSKFPHEKEGIIKFYSECWKIFNS---LNSLELKSL--------EEPIYLFGQFFKKPLECLTLAYYLPQNAGSIARKYIRDPGLLSFIDAECFIVSTVNALQTPMINASMVLCDRHF-GGINYPVGGVGEIAKSLAKGLDDHGSQILYRANVTSIILDNGKAVGVKLSDGRKFY---AKTIVSNATRWDTFGKLLK---AENLPKEEENFQKAYVKAPSFLSIHMGVKAD---------------VLPPDTDCHHFVLEDDWTNLEKPYGSIFLSIPTVLDSSLAPE-GHHILHIFTTSSIEDW------EGLSPK----DYEAKKEVVAERIISRLEKTLFPGLKSSILFKEVGTPKTHRRYLARDSG-TYGPMPRGTPKGLL--GMPFNTTAIDGLYCVGDSCFPGQGVIAVAFSGVMCA 584          

BLAST of predicted protein vs. SwissProt
Match: gi|209572620|sp|Q9M9Y8.2|CRTSO_ARATH (RecName: Full=Prolycopene isomerase, chloroplastic; Short=CrtISO; AltName: Full=Carotenoid and chloroplast regulation protein 2; AltName: Full=Carotenoid isomerase; Flags: Precursor)

HSP 1 Score: 108.227 bits (269), Expect = 2.219e-23
Identity = 150/558 (26.88%), Postives = 233/558 (41.76%), Query Frame = 0

Query:   94 DAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYG-ADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTL---------EIIQELTSDKDLQTVL-SYCWPDYGSKPSSSHFMMQSIL--NRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLP--KAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNERPGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQ-ACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTC-GFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIR 634
            DAIVIGSGIGGL AA  LA    RVLVLE++   GGS   Y   GY FDVG   +        L  + + +   +     +E+  D  ++ +   +N    ++ + + +IA L   FP E +GI GF+    +  N+   L +L+L  L           I++F + ++      TL          I ++   D  L + + + C+         +  +  S++  +RHY  G  YPVGG   IA ++   +   G ++  +A V+SI+ +  KA+GVR+  G E +   A  IIS A   +TF KLL   K   E   F ++          + +S+ +G+ A  E L               + D   ++  D    +      IF+S P+  D       GR         S  +W                EYE  K+ V   +I +   +L+P L   I + EVGTP T++ ++   +G  YG     T +  L   +   TT I GLY  G       G     F G+ CA  V         +GL K+ R
Sbjct:   79 DAIVIGSGIGGLVAATQLAVKEARVLVLEKYLIPGGSSGFYERDGYTFDVGSSVMFGFSDKGNLNLITQAL---KAVGRKMEVIPDPTTVHFHLPNNLSVRIHREYDDFIAELTSKFPHEKEGILGFYGDCWKIFNS---LNSLELKSL--------EEPIYLFGQFFQKPLECLTLAYYLPQNAGAIARKYIKDPQLLSFIDAECFIVSTVNALQTPMINASMVLCDRHY-GGINYPVGGVGGIAKSLAEGLVDQGSEIQYKANVKSIILDHGKAVGVRLADGREFF---AKTIISNATRWDTFGKLLKGEKLPKEEENFQKVYVK-----APSFLSIHMGVKA--EVL-------------PPDTDCHHFVLEDDWKNLEEPYGSIFLSIPTILDSSLAPD-GRHILHIFTTSSIEDWEGLPP----------KEYEAKKEDVAARIIQRLEKKLFPGLSSSITFKEVGTPRTHRRFLARDKG-TYGPMPRGTPKGLL--GMPFNTTAIDGLYCVGDSCFPGQGVIAVAFSGVMCAHRVAAD------IGLEKKSR 578          

BLAST of predicted protein vs. SwissProt
Match: gi|90101585|sp|Q2VEX9.1|CRTSO_DAUCA (RecName: Full=Prolycopene isomerase, chloroplastic; Short=CrtISO; AltName: Full=Carotenoid isomerase; Flags: Precursor)

HSP 1 Score: 107.842 bits (268), Expect = 3.046e-23
Identity = 150/576 (26.04%), Postives = 242/576 (42.01%), Query Frame = 0

Query:   63 RQKRRPYITEARERARIIQKSFHPDQV-----PSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYG-ADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTL---------EIIQELTSDKDLQTVL-SYCWPDYGSKPSSSHFMMQSIL--NRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPK--AVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPM--IFISFPSTKDPRWNERPGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQ-ACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTC-GFGGALFGGLFCA 614
            R+KR+P           + K    D+        + DAIVIGSGIGGL AA  LA  G +VLVLE++   GGS   Y   G+ FDVG   +        L  + + ++    +   +E+  D  ++ +    N    V+ +   + + L   FP E +GI  F+    +  NA   L +L+L  L           I++F + ++      TL         +I ++   D ++ + + + C+         +  +  S++  +RHY  G  YPVGG   IA ++   +   G ++  +A V+SI+    KA+GVR+  G EI+   A  IIS A   +TF KLL +     E   F +L          + +S+ +G+          K +V        + D   ++  D  D  + ++P   IF+S P+  D       G          S  +W    Q           +YE+ K+ V + +I +   +L+P L+  I   EVGTP T++ Y+    G  YG     T +  L   +   TT I GLY  G       G     F G+ CA
Sbjct:   65 RRKRKPNFV--LNSVLSVDKELESDETVGLGRSREYDAIVIGSGIGGLVAATQLAVKGAKVLVLEKYLIPGGSSGYYERDGFTFDVGSSVMFGFSDKGNLNLITQALAAVGCK---MEVIPDPSTVHFHLPSNLSVLVHREYNEFFSELTSKFPHEKEGIFKFYGECWKIFNA---LNSLELKSL--------EEPIYLFGQFFKKPMECLTLAYYLPQNAGDIARKFIKDPEVLSFIDAECFIVSTVNALKTPMINASMVLCDRHY-GGINYPVGGVGGIAKSLAKGLVDQGSEIQYKANVKSIIVENGKAVGVRLANGNEIF---AKNIISNATRWDTFGKLLKQDELPKEEENFQKLYIK-----APSFLSIHLGV----------KSDVLP-----PDTDCHHFVLED--DWSNLEVPYGSIFLSIPTVLDSSLAPE-GNHILHIFTTSSIEDWQGMSQ----------KDYEKKKELVADEIISRLEKKLFPGLQSSIVLKEVGTPKTHRRYLARDSG-TYGPMPQGTPKGLL--GMPFNTTAIDGLYCVGDSCFPGQGVIAVAFSGVMCA 584          

BLAST of predicted protein vs. nr
Match: gi|1325269061|ref|XP_023348951.1| (putative all-trans-retinol 13,14-reductase isoform X1 [Eurytemora affinis])

HSP 1 Score: 742.265 bits (1915), Expect = 0.000e+0
Identity = 336/621 (54.11%), Postives = 466/621 (75.04%), Query Frame = 0

Query:   19 WFFSNPEVISAIKVLVVLATGFLLVQVIRWSRMAEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYME-GAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNERPGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQEAK 638
            W F+ P +  AIK+ + L   FL +Q+ RWS  +++RNPL++D+R+KR+PY+ + ++R  I+ + F  D++PSDLDAI++GSGIGGLT AALLA++G+RVLVLEQHDQAGG CH++ +KGYEFDVGIHYIGQ+  PTI RTL +Q++EGQLEWEPL   +DVVSIG+G +NR+Y V  D + W   LK  FP+E  GI+ +F L+   + +T ++G LKL PLWLVKLV+ TGL+  FTKLW   F + T++++ ELT++KDLQT+ +YCW DYG+ PS S+F MQ++LNRH+ E GA YP+GGASEIA ++IPVIEK+GG+VLVRA V  I   G K  GV+VEKGG+  +I AP+IIS+AGV NTF+ LLPK ++  SYF ++  +  ++PGVA+MS+F+GLN S ++++ KK+NVW++  N  ++    Y D   ++ M+ ++P++FISFPSTKDP W   PGR +KST A+++ ANW W+K +++   KKRGD Y+ELKD +G+ M++   +L+P+L+  IDYVE+G+PVTN  Y+ +  GEIYGLDH   R +PL  A LRP TDIPGLYL+GQD+++CGF GALF G+  A A LGRNVM DL+ LHK+I  + K
Sbjct:    5 WLFTVPVLKLAIKIAIGLIAIFLSIQIFRWSMQSKSRNPLEKDVRKKRKPYVHDQKKRNEILCQGFSKDKIPSDLDAIIVGSGIGGLTTAALLAKSGKRVLVLEQHDQAGGCCHSFIDKGYEFDVGIHYIGQLNSPTINRTLCDQITEGQLEWEPLNPDYDVVSIGFGEENRKYLVKTDLQEWKNYLKTEFPEERSGIEKYFELVLSCNKSTTLMGILKLTPLWLVKLVLWTGLLKRFTKLWSKVFLTKTMDVVNELTTNKDLQTIFTYCWGDYGTPPSRSNFNMQAMLNRHFSECGASYPIGGASEIAFSIIPVIEKTGGRVLVRANVTEITTRGDKVCGVKVEKGGDKIEILAPIIISSAGVYNTFQSLLPKELSSKSYFTDI--ARTLKPGVASMSIFIGLNVSNDEIESKKQNVWSFPVNNTDQAALDYFDLSAEEAMTAEVPLLFISFPSTKDPEWANHPGRSDKSTLALVTLANWEWFKDWKDKGVKKRGDAYKELKDCIGHQMVEHTLKLFPELRDKIDYVEIGSPVTNNHYLAAPHGEIYGLDHCSERFDPLMIAKLRPETDIPGLYLTGQDILSCGFTGALFSGILTAQACLGRNVMADLINLHKKINSDKK 623          

BLAST of predicted protein vs. nr
Match: gi|1325269063|ref|XP_023348959.1| (putative all-trans-retinol 13,14-reductase isoform X2 [Eurytemora affinis])

HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0
Identity = 323/588 (54.93%), Postives = 447/588 (76.02%), Query Frame = 0

Query:   52 AEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYME-GAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNERPGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQEAK 638
            +++RNPL++D+R+KR+PY+ + ++R  I+ + F  D++PSDLDAI++GSGIGGLT AALLA++G+RVLVLEQHDQAGG CH++ +KGYEFDVGIHYIGQ+  PTI RTL +Q++EGQLEWEPL   +DVVSIG+G +NR+Y V  D + W   LK  FP+E  GI+ +F L+   + +T ++G LKL PLWLVKLV+ TGL+  FTKLW   F + T++++ ELT++KDLQT+ +YCW DYG+ PS S+F MQ++LNRH+ E GA YP+GGASEIA ++IPVIEK+GG+VLVRA V  I   G K  GV+VEKGG+  +I AP+IIS+AGV NTF+ LLPK ++  SYF ++  +  ++PGVA+MS+F+GLN S ++++ KK+NVW++  N  ++    Y D   ++ M+ ++P++FISFPSTKDP W   PGR +KST A+++ ANW W+K +++   KKRGD Y+ELKD +G+ M++   +L+P+L+  IDYVE+G+PVTN  Y+ +  GEIYGLDH   R +PL  A LRP TDIPGLYL+GQD+++CGF GALF G+  A A LGRNVM DL+ LHK+I  + K
Sbjct:    3 SKSRNPLEKDVRKKRKPYVHDQKKRNEILCQGFSKDKIPSDLDAIIVGSGIGGLTTAALLAKSGKRVLVLEQHDQAGGCCHSFIDKGYEFDVGIHYIGQLNSPTINRTLCDQITEGQLEWEPLNPDYDVVSIGFGEENRKYLVKTDLQEWKNYLKTEFPEERSGIEKYFELVLSCNKSTTLMGILKLTPLWLVKLVLWTGLLKRFTKLWSKVFLTKTMDVVNELTTNKDLQTIFTYCWGDYGTPPSRSNFNMQAMLNRHFSECGASYPIGGASEIAFSIIPVIEKTGGRVLVRANVTEITTRGDKVCGVKVEKGGDKIEILAPIIISSAGVYNTFQSLLPKELSSKSYFTDI--ARTLKPGVASMSIFIGLNVSNDEIESKKQNVWSFPVNNTDQAALDYFDLSAEEAMTAEVPLLFISFPSTKDPEWANHPGRSDKSTLALVTLANWEWFKDWKDKGVKKRGDAYKELKDCIGHQMVEHTLKLFPELRDKIDYVEIGSPVTNNHYLAAPHGEIYGLDHCSERFDPLMIAKLRPETDIPGLYLTGQDILSCGFTGALFSGILTAQACLGRNVMADLINLHKKINSDKK 588          

BLAST of predicted protein vs. nr
Match: gi|1191057475|ref|XP_020906857.1| (putative all-trans-retinol 13,14-reductase [Exaiptasia pallida] >gi|999986336|gb|KXJ25475.1| putative all-trans-retinol 13,14-reductase [Exaiptasia pallida])

HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0
Identity = 271/611 (44.35%), Postives = 404/611 (66.12%), Query Frame = 0

Query:   28 SAIKVLVVLATGFLLVQVIRWSRMAEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGH--KAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQ 635
            +A+ V V+L    ++V ++R ++ +  +NP  +D  +K  P I    ER RI+++ F   ++P +LDAIVIGSGIGG++ A LLAR+G++VLVLEQHDQAGG CHT+ +KG+EFD GIHYIG+M   T  + L++Q+++GQL W PLE  +D V+IG   + R YP+   RE +  AL   FPKE   ID +  LL +   +      LK  P +L + ++ TG+IH+FT  ++   +S T E++ ELT +++L+TVL+Y + DYG+ P    F M +IL  H+M G+ YP GGASEIAL +   +EK G  V+VRA V  I+ +    +A GVRV K      I AP+++S+AG  NT+ KLLP++V+  +    L  +  ++ GV A++V+ GL  S  DL LK  N+WA++ N  ++  + YL    ++  ++D+P++FISFPSTKDP W +R PG   KSTC I+SFA + W++++++    KRGD+YEELK  +G  M +Q CRL+P +K   ++ +VG+P++NQ+YI S +GEIYGLDH++ R  P T   LRP T IPGLYL+GQD++TCGF GA+F G+F A ++L RNVM D++ L + ++ 
Sbjct:   16 NALLVAVILGVVPIIVFLVRRAK-SXGKNPFSKDTTRKPEPIILNQAERDRILKQGFSAKKIPENLDAIVIGSGIGGMSCACLLARSGKKVLVLEQHDQAGGCCHTFHDKGFEFDTGIHYIGEMQNRTATKFLIDQLTDGQLLWAPLEKQYDTVAIGDANNPRLYPIMSGREEYRKALIEKFPKEKBAIDKYLELLKKMRKSVMSYFILKYLPRYLGQFLVSTGIIHLFTNYFKMSKKSLT-EVLDELTDNRELKTVLAYSFGDYGNIPKEGSFAMHAILTNHFMNGSSYPQGGASEIALRLTQALEKMGSAVMVRAHVTQILLDEETGRAKGVRVSKSSGDIDIRAPLVVSSAGFYNTYEKLLPQSVSPLNSI--LKTTKGVKHGVGAITVYXGLKGSKHDLGLKATNMWAFIDNNLDQISEKYLQMQPEEAGNQDVPLLFISFPSTKDPEWEKRFPG---KSTCEIVSFAPYEWFEEWKDKRVMKRGDDYEELKHRIGRRMWEQTCRLFPHIKDKEEFFDVGSPLSNQYYIASPRGEIYGLDHNKDRFSPETCVNLRPETGIPGLYLTGQDILTCGFAGAMFSGVFTASSILNRNVMSDMMNLREHVKH 619          

BLAST of predicted protein vs. nr
Match: gi|260801056|ref|XP_002595412.1| (hypothetical protein BRAFLDRAFT_119025 [Branchiostoma floridae] >gi|229280658|gb|EEN51424.1| hypothetical protein BRAFLDRAFT_119025 [Branchiostoma floridae])

HSP 1 Score: 550.821 bits (1418), Expect = 0.000e+0
Identity = 285/627 (45.45%), Postives = 402/627 (64.11%), Query Frame = 0

Query:   13 NVVAQGWFFSNPEVISAIKVLVVLATGFLLVQVIRWSRMAEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADN-RQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYM-EGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPV-QSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQ 635
            NV A   F S+P ++  +  L+ L      V ++  +R    +NP   D R+     +T+   R R+I+  F   +VP  LDA+V+GSGIGGL  AA++A+AG+RVLVLEQHDQAGG CHT+ EKG+EFDVG+HYIG+M   T+ + L +Q++EGQL+WE L+   DVVS+G  A+N R++P+    E +  AL   FP E + IDGF  LL +   +      L+  P WL KL++ TGL++  T  ++   +S   + + ELT ++DL+ VLSY + DYG+ P  + F M + L  HY   G +YP+GGASEIA ++IPVIEK+GG VLVRAPV Q ++ +   A GVRV K      I AP++IS AG+ NT++ LLPK VA       +L+  R+R GV A+S+FVGL  + E+L LK +N WA+ +N+ +  F+ Y     +D    D+P++FISFPS+KDP   +R PG   KSTC +I+   + W++Q+E+    KRG EY++LK  V   + DQ CRLYPQ+K  ++Y E+GTPVTN++YIG+ +GEIYG+DH+  R    T   L P T +P LYL+GQD+  CG  GALFG L  A AVL RNVM+DL+ L K+I +
Sbjct:    6 NVAAS--FMSSPGLLVLVVALIAL------VLMVTSNRKPRGKNPFATDCRRPAAALVTDQSARDRVIKNGFAIKKVPETLDAVVVGSGIGGLGVAAMMAKAGKRVLVLEQHDQAGGCCHTFIEKGFEFDVGVHYIGEMCGSTLNKVLFDQLTEGQLQWEKLDDELDVVSLG--AENPRKFPIVQGNEAFKKALLEKFPDEEKAIDGFLLLLKKVRKSMMGYVGLRSMPQWLAKLLVKTGLVNYMTDYFKYAGKSVK-QTLDELTDNQDLKAVLSYSFGDYGTPPKDAPFTMHATLISHYYYNGGYYPIGGASEIAFHMIPVIEKAGGAVLVRAPVNQILIDDSGVACGVRVHKSSGDIDIHAPIVISDAGLYNTYQSLLPKEVALKHGLDNVLS--RVRHGVGAVSLFVGLRGTKEELGLKAQNCWAFTNNDLDAGFEEYASRSAEDAGKSDVPLLFISFPSSKDPTHEQRYPG---KSTCTVITLCPYEWFEQWEDERVMKRGQEYKDLKMAVAARIWDQTCRLYPQIKDKVEYFEMGTPVTNKYYIGAPRGEIYGIDHNMARFSADTAIQLNPETPVPNLYLTGQDIFCCGLAGALFGSLLSANAVLNRNVMFDLLNLRKKIAK 616          

BLAST of predicted protein vs. nr
Match: gi|1229128343|ref|XP_022085438.1| (putative all-trans-retinol 13,14-reductase [Acanthaster planci])

HSP 1 Score: 550.051 bits (1416), Expect = 0.000e+0
Identity = 277/609 (45.48%), Postives = 398/609 (65.35%), Query Frame = 0

Query:   32 VLVVLATGFLLVQVI-RWSRMAEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVY-GDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGH-KAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQE 636
            V + L   +LL +++ +    + + NP  +D R+  +P  T+   + +++++ F   ++P  LDAIVIGSG+GGLT A++L+R G++VLVLEQHDQAGG CH + EKGYEFDVGIHYIG+M   TI +  ++Q+++GQL+W PL+   D V IG     RQYP+  G ++++  AL  +FP E + I+ F  LL      + + GA+KL P  +   +I  G+I+  +  +    RS   E++ +LT ++DL+ VLSY + DYG+ PS S F+M  +L  H+M+G F+PVGGASEIA ++IP IE+SGG+VLVRAPV  I+ +   KA GVRV+K      I AP+IISAAGV NTF++LLP          E   +  ++ GV AMSVFVGL  + E+L L    VWA+  ++ +K+ + Y++   ++    D+P++FI+FPS KDP W ER PG   KSTC +++ ANW W+ ++EN    KRG+EY+ +KD +G  M +Q   LYPQL+  +DY  VG+PVTN++YI + +GEIYGLDH++ R    T   LRP TDIP LYL+GQDVMTCGF GA F GL     +L RN+M D+V L K+I Q+
Sbjct:   21 VCLTLTFIYLLNKILNKLMITSNSPNPFAKDTRRPPQPLETDHAVKDKVLKQGFTAQKIPEGLDAIVIGSGMGGLTTASVLSRIGKKVLVLEQHDQAGGCCHIFREKGYEFDVGIHYIGEMTNNTISKVFIDQLTDGQLQWYPLDETFDTVVIGQEGKQRQYPIKAGPKDQYRRALLESFPGEEKAINKFMKLLRTVKRQSLIAGAMKLIPRNVCNFLIQYGIINFISSFYSYAHRSLK-EVLDDLTDNEDLKAVLSYNFGDYGTPPSKSSFVMHCLLVNHFMKGTFFPVGGASEIAYHIIPTIERSGGKVLVRAPVTQILLDSKGKACGVRVKKTSGDIDIHAPMIISAAGVYNTFQRLLPPTNPVVQKPIEKRHTG-VQHGVGAMSVFVGLKGTKEELGLPAGQVWAFTGSDHDKNMEEYMNRSAEESGKDDIPLLFITFPSAKDPSWPERYPG---KSTCTMVTLANWEWFSKWENGRTGKRGEEYDRIKDAIGEKMWEQCLNLYPQLRDKVDYFSVGSPVTNKYYIEAMKGEIYGLDHNKERFSTQTWHTLRPETDIPNLYLTGQDVMTCGFVGAAFSGLISCSKILSRNLMADVVALRKRIIQQ 624          

BLAST of predicted protein vs. nr
Match: gi|1126163392|ref|XP_019641120.1| (PREDICTED: putative all-trans-retinol 13,14-reductase [Branchiostoma belcheri])

HSP 1 Score: 547.354 bits (1409), Expect = 0.000e+0
Identity = 280/627 (44.66%), Postives = 405/627 (64.59%), Query Frame = 0

Query:   13 NVVAQGWFFSNPEVISAIKVLVVLATGFLLVQVIRWSRMAEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADN-RQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRH-YMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPV-QSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQ 635
            NV A   F SNP ++  +  L+ +      V ++  +R    +NP   D R+   P +T+   R R+I+  F   +VP  LDA+V+GSGIGGL  AA++A+AG+RVLVLEQHDQAGG CHT+ EKG+EFDVG+HYIG+M   TI + L +Q++EGQL+WE L+ A DVV++  GA+N R++P+    E +  AL   FP+E + IDGF  LL +   +      L+  P WL +L++ TGL++  T  ++   +S   + + ELT ++DL+ VLSY + DYG+ P  + F M + L  H Y  G +YP+GGASEIA ++IPVIEK+GG VLVRAPV Q ++ +   A GVRV K      I AP++IS AG+ NT++ LLPK VA       +L+  ++R GV A+S+FVGL  + E+L LK +N WA+  N+ +  F+ Y +   +D    D+P++FISFPS+KDP   +R PG   KSTC +I+   + W++++E+    KRG EY++LK +V   + DQ CRLYPQ+K  ++Y E+GTP+TN++YI + +GEIYG+DH+  R    T   L P T +P LYL+GQD+  CG  GALFG +  A +VL RNVM+DL+ L K+I +
Sbjct:    6 NVAAS--FMSNPGLLVLVVALIAI------VFMVTSNRKPRGKNPFAADCRRPPSPLVTDQAARDRVIKNGFVIKKVPETLDAVVVGSGIGGLGVAAMMAKAGKRVLVLEQHDQAGGCCHTFIEKGFEFDVGVHYIGEMCGSTINKVLFDQLTEGQLQWEKLDDAMDVVAL--GAENPRKFPIVQGNEAFKKALLEKFPEEEKAIDGFLLLLKKVRKSMLGYVGLRSLPQWLARLLVKTGLVNYMTNYFKYASKSVK-QTLDELTDNQDLKAVLSYSFGDYGTPPKDAPFTMHATLISHYYYNGGYYPIGGASEIAFHMIPVIEKAGGAVLVRAPVTQILIDSSGAACGVRVHKSSGDIDIHAPIVISDAGLYNTYQTLLPKEVAVKHGLDCVLS--KVRHGVGAVSLFVGLRGTKEELGLKAQNCWAFTHNDLDAGFEEYANRSAEDAGKADVPLLFISFPSSKDPSHEQRYPG---KSTCTVITLCPYEWFEKWEDERVMKRGQEYKDLKMSVAARIWDQTCRLYPQIKDRVEYFEMGTPITNKYYIAAPRGEIYGIDHNMARFSADTVTQLHPETPVPNLYLTGQDIFCCGLAGALFGSILSANSVLNRNVMFDLMNLRKKIAK 616          

BLAST of predicted protein vs. nr
Match: gi|156391123|ref|XP_001635618.1| (predicted protein [Nematostella vectensis] >gi|156222714|gb|EDO43555.1| predicted protein [Nematostella vectensis])

HSP 1 Score: 545.428 bits (1404), Expect = 0.000e+0
Identity = 272/595 (45.71%), Postives = 391/595 (65.71%), Query Frame = 0

Query:   45 VIRWSRMAEAR---NPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIV--HNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNR-IRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQ 632
            +IR+ R A ++   NP  RD R+   P + +  ER RI+++ F   ++P ++DA+VIGSGIGG+T A+LLA+ G+++LVLEQHDQAGG CHT+ EKG+EFD GIHYIG+M   + ++ L++Q+++GQL W PLE  +D V+IG  A  + +P+   RE +  AL   FPKE + ID +  LL E       L  +K  P WL  L+I TGL+H  T  ++   +S T  ++ ELTSDKDL+ VL+YC+ DYG+ P+ + F M + L  H++ G  YP GGASEIA ++I  ++K+   +LVRA V+ I+         GVRVEK      I AP+++S AG  NT++KLLP++V   S   ++L+  R I+ G+ AMSV+VGL+ + E+L LK  N+WA+  N+ +     YL  D     + D+P++FISFPSTKDP W++R PG   KSTC II+ A + W++Q+EN    KRG +YE  K  +G  M +Q CR +P L+  ++Y +VG+PV+N +YI S +GEIYG+DH+  R  P +   LRP T IPGLYL+GQDV++CGF GA+FGGLF A  +L RN+M DL+ L K+
Sbjct:   35 IIRFFRKAFSKRGPNPFARDTRRAPEPLVLDQSERDRILKQGFVAKKIPDEVDAVVIGSGIGGMTCASLLAKTGKKILVLEQHDQAGGCCHTFHEKGFEFDSGIHYIGEMMSNSAIKFLIDQITDGQLLWSPLEKHYDTVAIGDMAKPKLFPIMSGREEFRNALYEKFPKEKKAIDQYIVLLKEARKNMVGLVTIKSLPKWLGNLLIRTGLVHYVTGYFKMSAKSLT-HVLNELTSDKDLKAVLAYCFGDYGTLPADASFAMHATLVNHFLYGCAYPRGGASEIAFHIIRSLQKNNSTILVRANVKQILLDETTGSVKGVRVEKKSGDIDIYAPLVVSGAGFFNTYKKLLPQSV---SPLKQVLSETRGIQHGIGAMSVYVGLHGTKEELGLKARNMWAFTGNDLDSICSEYLGLDGDAAGNEDIPLLFISFPSTKDPEWDKRHPG---KSTCTIITLARYEWFEQWENERVMKRGADYELRKQRIGKRMWEQTCRFFPNLEDKVEYFDVGSPVSNNYYIASPRGEIYGIDHNVERFSPESAVNLRPETAIPGLYLTGQDVLSCGFAGAMFGGLFTASTILNRNLMADLMDLRKR 622          

BLAST of predicted protein vs. nr
Match: gi|443686691|gb|ELT89885.1| (hypothetical protein CAPTEDRAFT_174975 [Capitella teleta])

HSP 1 Score: 543.502 bits (1399), Expect = 0.000e+0
Identity = 281/619 (45.40%), Postives = 405/619 (65.43%), Query Frame = 0

Query:   19 WFFSNPEVISAIKVLVVLATGFLLVQVIRWSRMAEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPV-YGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHN-GHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTR-QEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQI 633
            +  ++P ++ ++ +L V    F+ V  I  S     RNP   D  +K +  +T+   R + +++ F P +VP ++DAIV+GSGIGGLT+AA+LA+AG++VLVLEQHDQAGG CHT+ +KG+EFDVGIHYIG+M   T+ R LVEQ+++GQL+W  L    DVV+IG   + +  P   G+ + W   L   FP+E   ID + A+L E   +T  L   K+   W+V+ +++TGLIH  TK ++   R S  E+++E+T +KDLQ V SY + DYG+ PSS+ F M + L  HYM GA YP GG+SEIA ++IPVIEK+GG+VLVRA V  I+ +   K  GVRV +      I AP++IS AG VNTF+ L+PK +A  S   EL+ S R+  GV  MSVF+GLN + E+LK+K +N W++ +N+ ++  + YL    +++M  D+P++FISFPS+KDP +N+R PG   K+ C II+ A W W+ Q+++    KRG +Y+ +K+ +G  M  QA  ++PQLK  + Y +V +P TN +Y+ S +GEIYGLDH + R  +P     LR  T IPGLY++GQD   CGF GA+FGGLF AG VL RN+  DL+ L K +
Sbjct:   10 YLVAHPGLVVSLFLLYV----FIYVLSILLSGRKPGRNPFRTDSARKPKELVTDQATRDQRLKQGFLPKKVPENVDAIVVGSGIGGLTSAAVLAKAGKKVLVLEQHDQAGGCCHTFIDKGFEFDVGIHYIGEMRSNTVGRLLVEQITDGQLQWVDLIDDFDVVAIGKAGEQKMIPFRTGNWKIWRENLVKHFPEEEVAIDKYMAMLKECRKSTLGLVLAKVMNKWVVRFLVNTGLIHWMTKYFKIA-RRSLKEVVEEVTDNKDLQCVFSYSFGDYGTVPSSASFAMHAALINHYMYGASYPRGGSSEIAHSIIPVIEKAGGKVLVRAKVTEILFDEAGKTKGVRVGRSSGDVDIFAPIVISDAGAVNTFKSLVPKEIAAKSSITELIGS-RVTSGVGCMSVFIGLNGTCEELKIKPQNFWSFNNNQIDQSARDYLKLSAEEVMEADVPLMFISFPSSKDPTFNDRYPG---KTVCEIITLAKWEWFSQWKDGRVMKRGSDYDAVKNAIGERMWQQALGIFPQLKDKVAYFDVASPATNNWYLNSPKGEIYGLDHTKERFGDPDIAMRLRAETGIPGLYMAGQDAFACGFMGAMFGGLFAAGKVLNRNLYDDLLRLKKLV 619          

BLAST of predicted protein vs. nr
Match: gi|1126194714|ref|XP_019630441.1| (PREDICTED: putative all-trans-retinol 13,14-reductase [Branchiostoma belcheri])

HSP 1 Score: 525.783 bits (1353), Expect = 1.225e-176
Identity = 282/635 (44.41%), Postives = 394/635 (62.05%), Query Frame = 0

Query:   22 SNPEVISAIKVLVVLATGFLLVQVIRWSRMAEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGR----PTILR------TLVEQVSEGQLEWEPLELAHDVVSIGYGADNR-QYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTLEIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPV-QSIVHNGHKAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDL--PMIFISFPSTKDPRWNER-PGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTR--QEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGLHKQIRQEAKA 639
            S P V   I V V          +++W R     NP   D  +   P +T+  +R +++++     +VP+DLDAIV+GSGIGGL  AALLA+ G++VLVLEQHDQAGG CHTY +KG+EFDVGIHY G+       P +L+       L++Q++EGQL+W  L+ + D+++I +  D R + P+   R      L   FP E + ID FF L D  +   +    +K  PLWLVKL I TGL+   T  +    RS T E++   T ++DL+ VLSY + D+GS P  S F+MQ +  +H+ EGAFYP GGASEIA ++IPVIE++GG VLV APV Q ++ +  KA GVRV K      I APV++SAAGV NT++KL+P+ V       E+    ++RPG+ A+SVFVGL  + ++L LK  N W +  N+ ++  + Y    +++    +   P++F+SFPS KDP WN R PG   KSTC IISF  + W+ +++    K RG+EYE LK  +   M  Q C+ YPQL+   +Y++VGTP++N +Y+GS++GEIYGLDH+R R  Q+ L N  LRP T IPGLYL+GQD++T GF GAL  GL  AGAVL R ++ DL+ L      EAKA
Sbjct:   16 SAPVVFGLICVTVTSVAAL----IVKWHR--SPANPFSADRARSPEPLVTDHAKRDKVLKQRLTTKKVPADLDAIVVGSGIGGLCTAALLAKTGKKVLVLEQHDQAGGCCHTYVDKGFEFDVGIHYTGKQNSVRTWPQVLQGGTVNQALIQQITEGQLQWAELDKSFDIITIQHDQDKRIEVPIKSSRSEHHKTLLEHFPTEGKAIDEFFRLCDVYTGTLSGFILMKTLPLWLVKLFIRTGLVSRLTSFFHYMSRS-TREVMDSFTDNQDLKAVLSYNYGDFGSPPKVSGFLMQGMTAQHFHEGAFYPRGGASEIAFHIIPVIERAGGAVLVAAPVNQILIDSDGKAFGVRVHKSSGDVDIHAPVVVSAAGVCNTYQKLVPQPVVRKHGLDEMF--QQVRPGLGALSVFVGLTGTSQELGLKAHNAWMFPGNDHDQLIEDYSRLTLQEACEEETKTPVLFVSFPSAKDPTWNTRYPG---KSTCIIISFTPYEWFAEWQEGRVKHRGEEYESLKTRLAQKMWRQVCQQYPQLQGKDEYMDVGTPLSNNYYLGSTRGEIYGLDHNRERFSQDLLVN--LRPDTAIPGLYLTGQDIVTAGFAGALVSGLLTAGAVLNRQLILDLIIL------EAKA 630          

BLAST of predicted protein vs. nr
Match: gi|1160114909|ref|XP_020366885.1| (all-trans-retinol 13,14-reductase isoform X1 [Rhincodon typus] >gi|1160114911|ref|XP_020366886.1| all-trans-retinol 13,14-reductase isoform X2 [Rhincodon typus])

HSP 1 Score: 522.316 bits (1344), Expect = 1.150e-175
Identity = 267/598 (44.65%), Postives = 389/598 (65.05%), Query Frame = 0

Query:   34 VVLATGFLLVQVIRWSRMAEARNPLDRDLRQKRRPYITEARERARIIQKSFHPDQVPSDLDAIVIGSGIGGLTAAALLARAGRRVLVLEQHDQAGGSCHTYTEKGYEFDVGIHYIGQMGRPTILRTLVEQVSEGQLEWEPLELAHDVVSIGYGADNRQYPVYGDRERWIAALKIAFPKEYQGIDGFFALLDETSNATAVLGALKLAPLWLVKLVIHTGLIHVFTKLWRGQFRSSTL-EIIQELTSDKDLQTVLSYCWPDYGSKPSSSHFMMQSILNRHYMEGAFYPVGGASEIALNVIPVIEKSGGQVLVRAPVQSIVHNGH-KAMGVRVEKGGEIYQIEAPVIISAAGVVNTFRKLLPKAVAETSYFHELLASNRIRPGVAAMSVFVGLNASGEDLKLKKENVWAYLSNEGEKDFQTYLDSDVKDIMSRDLPMIFISFPSTKDPRWNERPGRENKSTCAIISFANWNWYKQFENSSHKKRGDEYEELKDTVGNIMIDQACRLYPQLKHHIDYVEVGTPVTNQFYIGSSQGEIYGLDHDRTRQEPLTNALLRPTTDIPGLYLSGQDVMTCGFGGALFGGLFCAGAVLGRNVMYDLVGL 629
            +VL   F+  +V R  R + + NP  RD R+   P +T+   R R+I K F  D+VP +LDAIVIGSGIGGL+ AA+LA+AG+RVLVLEQHDQAGG CHT+ +KG+EFDVGIHYIGQ+   + LRTL+EQ+++GQLEW  ++   D V +G     R Y VYG  + + + LK+ FP+E + ID F   +   S   ++L  +K+ PL L + +I +G+IH  + +++    S+ L +++ +LT +KDL+TV+ Y   DYG  P  + FMM +++ + Y  GA+YP GG+SEIA ++IPVI+KSGG VL+RAPVQ I+ N   +A GV V+KG E   + APV+IS AG+ NT+ +LLP+ +         L+   +R G+   SVFVGL+ S E+L LK +N W Y  N  ++ F  Y     +DI +   P +F+SFPS KDP W+ R     KS   I++ A++ W++++++   KKRG++YE LK  +   ++D+    +PQLK  I+Y+ VG+P++++FYI SS+GEIYG +HD +R +P   A LR  T I  LYL+GQD+  CGF GA+ GGL CA  VL RN+  D++ L
Sbjct:    7 LVLILYFIFHKVFR--RSSPSLNPFARDTRRPPEPLVTDPEARKRLIHKGFTADKVPENLDAIVIGSGIGGLSVAAILAKAGKRVLVLEQHDQAGGCCHTFIKKGFEFDVGIHYIGQLYERSFLRTLLEQITDGQLEWVKMDDLFDNVILGDQQTQRIYQVYGGNQNYRSCLKMQFPQETEAIDQFLEHIKMVSKKASLLVIIKMIPLPLARFLIKSGIIHWISSIFK--LASTPLSDVVNKLTQNKDLRTVMCYVCGDYGVIPKEASFMMHALVVQSYQSGAWYPKGGSSEIAFHIIPVIKKSGGDVLMRAPVQRILINKDGQACGVTVKKGKEEINLFAPVVISDAGIFNTYERLLPQELQRKPAIQAQLS--MVRHGMGGFSVFVGLDGSKEELGLKAQNYWFYHKNNIDELFSKYCTLSKEDI-TEGYPFMFVSFPSAKDPAWDAR--YTGKSNMVIVTLAHYEWFEEWKDERVKKRGEDYESLKMDIARKLMDRVIEYFPQLKDRIEYIRVGSPLSHEFYIASSRGEIYGANHDVSRFKPEVIASLRAGTPIKNLYLTGQDIFFCGFTGAIHGGLICASRVLHRNLYNDIMNL 595