af190129_1na+ k+ 2cl- cotransporter, snap_masked-scaffold609_size125094-processed-gene-0.16 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000003059 (supercontig:LSalAtl2s:LSalAtl2s1734:14347:19068:1 gene:EMLSAG00000003059 transcript:EMLSAT00000003059 description:"maker-LSalAtl2s1734-augustus-gene-0.5") HSP 1 Score: 805.053 bits (2078), Expect = 0.000e+0 Identity = 448/1006 (44.53%), Postives = 625/1006 (62.13%), Query Frame = 0
Query: 38 YVYDADQSFRK-LTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLP-AKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASG--IEAEY----LAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDPAGFESLAKVIHHSFDINMAVAILRLQNGCD--------------YSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSN--------KRVPKTVLLG---SDGKPLPAKLVQEINAFKTKKRV--GFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQAN----------------------------ISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
+ + +S R LT EA+P HYR S +SS RPT+ +L + ++ ++ES + +D G VIKFGWI+GV GL +I++ N+VT +T++SMSAVSTNG I+ GGIY+MISRSLG EFGGAIG+MFT+ANSIAVA YIIGF ++L DML + +PGF GI +T + L+D+R IG+V+L +L LA+VGMEWVTRV+K LL+LL+ SQ+DF+IG+FLP ++ + GFVGYN +NL + + KN FF V AVFFPAVTGIVAGANLSGDL+DP +AIPKGTL +V TY +Y IY +I + EASG +E +Y + + N+T+ F C R C YG + QQ + IS G L+YAGCFAATLSSAIASLVGAPRV QA+AKD L+P + F G+G NNDP++GY++VF I+ CI+IG+L+ VSSLLSNFFVA YALINFSVFHASITKSPGWRP FKYYNKW+SL T+LC+ VMFLM VTA++TF+ + LY+++S RKPE NWGSS AQ F+T+L ++ +L V+ HVKNYRP I+++SG P +R L+DFANL+TK++SLL V E + K ++ + + ++W ++ IK FFS+ + EGA +++ L+GLGKL+ N++++G++ W + + K+I +FD+ +AV +LR+ G D SN + + + S D G ++ ++++E+ K+ K N K+ P ++ L D KPL +V ++ F+ K V G IDV+WLYDDG HIL RA+++ C MRVF L+ KND LD Q+RSMA+LLAKFRIDF DV+M+ D K+ KDEFK +L + +S+ ++++N EKTN +LR+AE+++++SS ++ +
Sbjct: 100 FQFTTQRSLRHYLTREAIPHADHYRNKMSFAKGKPDSS--RPTLDDLLNDDPYSKDNS---DLESQTDDQD------TGSVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLAIITLSNIVTVITSISMSAVSTNGQIKAGGIYFMISRSLGPEFGGAIGLMFTLANSIAVAMYIIGFCEALSDMLKQYIPGFIGILNTPEDGLHDIRLIGSVSLVLLLGLAIVGMEWVTRVQKGLLVLLLFSQLDFIIGSFLPPSEDKLAKGFVGYNGTVLMENLFSEYIPNEGTEKNN-SFFTVFAVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLXIVSTYVTYTIYGVMIAAXTXREASGDVLELDYVNETIKEVNNITNS----FWECENRTCSYGILHSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVFQAVAKDKLFPGIASFADGWGKNNDPVKGYLLVFLISLICILIGDLNYVSSLLSNFFVAAYALINFSVFHASITKSPGWRPGFKYYNKWISLLGTVLCICVMFLMDMVTALVTFIIVFFLYMYVSIRKPEVNWGSSTQAQSFMTALNSVLSLTKVEDHVKNYRPKIMVMSGSPLNRPTLIDFANLVTKRISLLESVDVVPEDLDWKKSQAIKNSGQNWFTENHIKGFFSVVRNSKISEGARTAMELSGLGKLRTNMILLGFQDTWWKKDESAKEYYKIIQTAFDLRLAVGVLRINGGLDVTSMTNLMEDSSPNQSNALNYDDRCESNMSVDSGLDLATKSGAKNTSTQSGEDDKEDGSLKRNKKPNGFEKLLFKKKCPSSIPLPPSEKDVKPLDEDIVNRLSQFRDKTTVNTGTIDVYWLYDDGXXXX---HILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVPPLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLI 1086 HSP 2 Score: 122.865 bits (307), Expect = 1.969e-28 Identity = 65/160 (40.62%), Postives = 94/160 (58.75%), Query Frame = 0
Query: 954 TKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQAN----------------------------ISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
T G IDV+WLYDDG HIL RA+++ C MRVF L+ KND LD Q+RSMA+LLAKFRIDF DV+M+ D K+ KDEFK +L + +S+ ++++N EKTN +LR+AE+++++SS ++ +V+
Sbjct: 932 TTVNTGTIDVYWLYDDGXXXX---HILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVPPLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLIVM 1088
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000009267 (supercontig:LSalAtl2s:LSalAtl2s58:680408:685072:1 gene:EMLSAG00000009267 transcript:EMLSAT00000009267 description:"augustus_masked-LSalAtl2s58-processed-gene-6.1") HSP 1 Score: 486.878 bits (1252), Expect = 6.927e-156 Identity = 271/482 (56.22%), Postives = 333/482 (69.09%), Query Frame = 0
Query: 37 TYVYDADQSFRK-LTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL--------GLL-------------------VISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRE----CQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMI 486
TY +SFR+ LT +ALPSE HYR L SI + P S RPT+ EL + ++ ++ + +VE + KGKV++FGWI+GV G++ VI++ NLVT +TTLSMSAVSTNG IRGGGIYYMISRSLG EFGGAIGIMFT+ANSIAV+ YIIGF SLLDM + F+ ND+R IGA++L VL LA+VGMEWVTRV+ +LL LLI SQ+DFV+G+FLP K +A YGFVGYN F NL T YHDYK+P NPP FF V VFFPAVTGIVAGANLSGDL+DP +AIPKGTL A+++T+ +YI Y T+I +EASG + EY ++ V + L+F+NC+ R CQYGSSNDQQ + +IS TGYL+YAGCFAATLSSAIASLVGAPRVLQALAKD LYP++ +F GFGANNDP+RGY +VF I+ CI+I
Sbjct: 53 TYSTQYFKSFRQYLTRDALPSESHYRNLLSISNHPRKFS--RPTLEELQE---EQAENGRLLDKKDMDDVEKNEEEQSKGKVVRFGWIEGVFMRCLLNIWGVMLFLRLTWVVGQGGLIQGLGVIALGNLVTVITTLSMSAVSTNGQIRGGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCQSLLDMCKQY---FELEHLIFSDTTNDVRIIGAISLVAVLALAIVGMEWVTRVQMILLFLLIGSQIDFVVGSFLPTKEDAAYGFVGYNGTLFVDNLSPT-YHDYKDPGNPPSFFSVFGVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLAIIVTFITYIGYGTIITGVMHTEASGNQTEYENWINGV--EGALYFNNCSGRPADDFCQYGSSNDQQAMERISYTGYLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIHIFAPGFGANNDPIRGYFLVFIISMGCILI 523 HSP 2 Score: 406.757 bits (1044), Expect = 1.367e-125 Identity = 193/382 (50.52%), Postives = 260/382 (68.06%), Query Frame = 0
Query: 583 QQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKS---------NKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQAN--ISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
QQFVT+L+++Q+LN +HVKNYRP +L+ +GLPAHR PLVDFANL+TKKLSLL+CAHV P K E + + ++ W DH+IK+F+S+T ++NF EGA++ +NL G+GK++PN+V+ GYK DW+EDP G + V+HH+FD+++ V ILRL++GCD+S I E + +DEE E+E+ K+ KS ++V V G DG PLP VQ+I F+ KKR G IDVWWLYDDGGL+LLLP+ILT RAQFA+C +RVF+LA + D LD ++R+MA LLAKFRI + V +IPD+ KKA DP K+EFK IL + + + E++S EKTNRHLRL ELLREHS SE +
Sbjct: 539 QQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHVNIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLAGIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKVIGEEEQQQ--------------------HEIELDEENEDEKNGKQGKSLELQQKKVRXRKVSTAVYTGIDGNPLPKNTVQDITQFQIKKRKGNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKILDEIKPPLEESEIISQKEKTNRHLRLTELLREHSKRSEMI 900 HSP 3 Score: 177.178 bits (448), Expect = 1.441e-45 Identity = 82/133 (61.65%), Postives = 103/133 (77.44%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQAN--ISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
KKR G IDVWWLYDDGGL+LLLP+ILT RAQFA+C +RVF+LA + D LD ++R+MA LLAKFRI + V +IPD+ KKA DP K+EFK IL + + + E++S EKTNRHLRL ELLREHS SE +++
Sbjct: 770 KKRKGNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKILDEIKPPLEESEIISQKEKTNRHLRLTELLREHSKRSEMIIM 902
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000003697 (supercontig:LSalAtl2s:LSalAtl2s1:1949199:1954526:1 gene:EMLSAG00000003697 transcript:EMLSAT00000003697 description:"maker-LSalAtl2s1-augustus-gene-19.17") HSP 1 Score: 253.062 bits (645), Expect = 6.720e-71 Identity = 175/613 (28.55%), Postives = 300/613 (48.94%), Query Frame = 0
Query: 118 IKFGWIDGVLG----LLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCV----LILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKY------GFVG-----YNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGK--GFG-ANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPV-TAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLD--DH-RIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGY 704
++ G+I G G LL I + + T LS+SA++TNG ++GGG+Y+MISR+LG EFGG+IG++F AN + A Y + + ++ E + T+ H L + W + + L + ++G + ++L+ ++ + + F + E + F G ++ E F +L + D + P F V AV F VTGI+AGANLSGDL++P+ +IPKGTL A T+ +Y+I TL A + E L +R+C Y + + S V G AT ++ + L+G+ RVL+A++KD ++ G+F + +G N+P+ VV + ++ IG ++ ++ ++ F+ YA +N S + +P +RP F+ ++ + I + +MF+++PV +A++ F CI + NWGS A F + L L++ + HVK +RP +LLL P + L+DFAN L KK L + HV + + ++ + HWL+ DH ++KAF +T + G I ++G+G +KPN ++ G+
Sbjct: 44 LRVGYILGNAGILHSLLQIVIAYGILVFTILSVSAIATNGAVKGGGVYFMISRTLGPEFGGSIGVLFYAANIASSALYAVACTECIISSFGEE-------SGTLAHYLPESFWYQILYRVLINLLNLSICLIGPNLFGKTTIMVLISVVFCTLTVITSFFSASTVEVHFNETISGSFTGILSKNWSIENFQSHLYPSYSLDCNDDTKPVNFAIVFAVLFSGVTGIMAGANLSGDLKEPSKSIPKGTLLASFFTFITYVILFTL-------TAMTCDKELL------------------LRDCSY--------MIEFSFWKPFVIIGTVLATFCASTSCLIGSSRVLEAISKDTMF---GIFLEWITYGTVGNNPVLAVVVTWLLSNIFFFIGGVNTIAQFVAVLFLLSYASVNLSCLSLDLASAPNFRPIFRCFSWYTCALGLIGTLTMMFMINPVISALVLFACIFFFLSLSFLSPTKENWGSISQALLFHQVRKYLLLLDSRKDHVKFWRPQVLLLVSNPRNHCSLIDFANAL-KKGGLYVLGHVREGNFEGMEEDECGLLMPHWLNLVDHLKVKAFVELTVAPTVRSGIQQLIRISGIGAMKPNTIIFGF 612
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000004234 (supercontig:LSalAtl2s:LSalAtl2s222:1210791:1213007:1 gene:EMLSAG00000004234 transcript:EMLSAT00000004234 description:"maker-LSalAtl2s222-snap-gene-12.7") HSP 1 Score: 221.09 bits (562), Expect = 1.084e-61 Identity = 166/606 (27.39%), Postives = 281/606 (46.37%), Query Frame = 0
Query: 147 SAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLS--ENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQV--------------DFVIGTFLPAK------------------SEAKYGFVGYNTET-----FTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLY-PYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKP--EANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWL---DDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSD 707
SA+S+NG + GGG+Y+M+SR+LG EFGGAIG +F AN ++ A Y+ G + L+ P + + T + LN L + +VG ++ + L+ +++V + DF+ P K S K+ +G ET F NL +D FF + V F VTGI++GAN+ G+L P+ +IP+GT++A T YII L L ++T L +C Y ++ ++V V G F AT S+++ +L+G R+L+A+AKD ++ P KG N P+ + +F+ +M+G+L+ ++ L S F+ Y IN + + +P +RP++KY++ + L + + +MF +S + + + + L L ++ P NWGS A F + L L+ + HVK +RP +LLL P L++F N L KK L + HV +++ + + + I HWL D ++KAF +T +++ G + ++G+G +KPN V++G+K D
Sbjct: 16 SALSSNGAVEGGGVYFMLSRTLGPEFGGAIGTLFFFANVVSSALYLAGCTEGLVSNFKFLARGPWIEFLYSTSINSLN-------------LFICLVGSKFFGKTSVLIFFMVLVCVLMTSVSFFLDYTITEDFIFNATDPLKCVQPSKNDTNQTPNCTLTSTGKFTGIGSWNETILVQNFYDNLYPKYEYDCSGSDKSLNFFIIFGVLFSGVTGIMSGANMCGELVSPSKSIPRGTISACGFTLVIYII--------------------LTVLTSLTCSPTLL-----QHDCMY--------MNIVNVLPPAVTIGVFMATFSASLNNLIGXSRILEAIAKDVVFGPLFNFVLKGTYKEN-PVTAVISTWFVVELFLMMGSLNNIAQLCSVLFLLSYGTINLTCLFLHLASAPNFRPTYKYFSTYTCLIGLLGSIIMMFAVSAIYSAVGILLCLSLILLLNLFSPIRHTNWGSISQALIFHQVRKYLLLLDQRKSHVKFWRPQMLLLVSNPRRNCSLIEFGNAL-KKSGLYVLGHVHVDNLEEHDKDPTLNEIPHWLKLIDFLKVKAFVEVTIARSLRLGVDQLVRISGIGAMKPNTVLLGFKDD 573
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000007778 (supercontig:LSalAtl2s:LSalAtl2s455:327857:377250:-1 gene:EMLSAG00000007778 transcript:EMLSAT00000007778 description:"maker-LSalAtl2s455-augustus-gene-2.20") HSP 1 Score: 193.356 bits (490), Expect = 1.968e-50 Identity = 127/453 (28.04%), Postives = 210/453 (46.36%), Query Frame = 0
Query: 309 FFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYI----IYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLL----SGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSH--IEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWM-EDPAGFESLAKVIHHSFDINMAVAILR-LQNGCDYSNKIKS 749
F ++ +FFP+ TGI+AG+N SGDL P IP GT+ A++ T Y+ ++A + L + G +GN+ +V G +T+ + + SL GAPR+LQA+AKD + P+L F + A +P+R + I ++IGN+D ++ LLS FF+ Y +N + ++ ++P WRP FKYY+ +SL +LC+AVMF+ S A++ I+Y +I YR E WG + +L L H KN+RP IL+L S L L A+ L L I V + E ++ I+ + D +K F + S++ +G TGLG +KPN V++G+ + W ++ ++ + ++ MA+ + + +Q D +K+K
Sbjct: 449 FVILVGIFFPSCTGIMAGSNRSGDLAXPQKXIPIGTIMAILTTSTVYLSSVLLFAATVDPLLLRDKFGESIGGSLVVGNIAWPHP-----------------------------KVVEIGSLLSTIGAGLQSLTGAPRLLQAIAKDGIIPFLAPF-QVQSAKGEPVRALGITLLICEVAVLIGNVDEIAPLLSMFFLMCYGFVNLAXALQTLLRTPNWRPRFKYYHWSLSLLGVVLCIAVMFMSSWYYALLAMGIAGIIYKYIEYRGAEKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILAKLDSQLKVKHKRLFALASQLKAGKGLTIGVTVLDGHFGKMYGEAQAAKFSIQQVIKDEAVKGFADVVVSKDISDGVCHLTQTTGLGGMKPNTVILGWPNSWRKKEEDSWKVFVDSVRYASAAKMALLVPKGIQRYPDSGDKVKG 871 HSP 2 Score: 80.8777 bits (198), Expect = 1.179e-15 Identity = 59/178 (33.15%), Postives = 86/178 (48.31%), Query Frame = 0
Query: 118 IKFGWIDG----VLGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVA-----------TYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKYGF 280
I+ WI G V G V+ + VT LT +SM+ ++TNGV+ GGG +MISR+LG EFGGA+GI F A +A A YI+G SL G D D V + N+ R G L + + +G+ +V + + L +++S V +G F+ K F
Sbjct: 168 IRMTWIVGTAGAVEGFFVVFMGCCVTLLTAISMAXIATNGVVPGGGSXFMISRALGPEFGGAVGIXFYXAXXVAAAMYIIGAIEILIIYIVGPAMSLF--------GPDITNDFV--KFNNYRVYGTFLLITMGTIVFIGVRFVNKFAAVALACVLLSIVSIYVGIFVNIGGNDKANF 335
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709294|sp|P55013.1|S12A2_SQUAC (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; AltName: Full=NKCC; AltName: Full=Na-K-CL symporter) HSP 1 Score: 654.055 bits (1686), Expect = 0.000e+0 Identity = 386/1031 (37.44%), Postives = 587/1031 (56.94%), Query Frame = 0
Query: 13 KSEKGEPPNGGFYQSTSISENNMNT------YVYDAD------QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPK--TPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASG-----IEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEH--WLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDW----MEDPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSN----------KIKSEH----LVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPL---PAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL---------------------TQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
+S G P NG T +SE ++++ + YD ++F T++A+P HYR + + + RP++ EL E ++ E E+ K V+KFGWI GVL LLVI +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGGAIG++F AN++AVA Y++GF +++ D+L E+ A +D ++D+R IG+VT+ + ++V GMEW + + +LL +L+++ V+F +GTF+PA + GF Y E F++N + D+++ ++ FF V A+FFPA TGI+AGAN+SGDL DP +AIPKGTL A+++T Y A +GSC + EA+G I + NV K G F +C +C YG ND Q +S +S G L+ AG F+ATLSSA+ASLV AP++ QAL KDN+YP L VF G+G NN+PLRGYV+ FFI I+I L+V++ ++SNFF+A YALINFSVFHAS+ KSPGWRP+F++YN W+SL ILC VMF+++ A++T V + LY++++Y+KP+ NWGSS A ++ +L++ L V+ HVKN+RP LL++G P R L+ + TK + L++C HV Q + E + ++ WL +++KAF++ ++++ EG + GLG+++PN ++ G+K DW M+D E+ IH +FD V ++RL+ G + S+ + KS H +V + SDADS V E + A+ +++++EE+ K+ + + K V PL KL+Q + F+ K+ G IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF + K + +D R+MA LL+KFRIDF D+ ++ D+ K S F+ ++ I+D E+ KT R +RL ELLRE+S + +
Sbjct: 144 ESMNGYPQNG----DTMMSEGSLHSSGTGAHHYYDTHTNTYYLRTFGHNTIDAVPRIDHYRHTVAQLGE----KLIRPSLAELHDELDKEPFEDGYVNGEESSPAEEAVSKHVADNKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGHAGIGLALLVIGTATVVTTITGLSTSAITTNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVRDLLVEHN------ALMID-EMSDIRIIGSVTIVVLFGISVAGMEWEAKAQIVLLGILLLAIVNFTVGTFIPANDKRAKGFFNYRGEIFSENFVP----DFRDGED---FFSVFAIFFPAATGILAGANISGDLADPQLAIPKGTLLAILITTIVYAGAAVSVGSCIVREATGNLTDAIIPGTVTNCTNVACKLGFNFSSCATNKCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHVFSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFRFYNMWISLIGAILCCGVMFVINWWAALLTNVIVLALYIYVTYKKPDVNWGSSTQALTYLNALQHAIRLTGVEDHVKNFRPQCLLMTGAPTSRPALLHLVHAFTKNVGLVVCGHVHTGPRRQALKEISTDQAKYQRWLIKNKMKAFYAPVYAEDLREGTQFLLQAVGLGRMRPNTLVFGFKKDWRQALMKD---VENYINAIHDAFDYQYGVVVIRLKEGFNISHLQAQEELCTSQEKSAHPKDIVVNLEHSDADSSKPSSKS-VSETNSPAVCQDQKDEEDDGKASTQPLLKKEV--KDPSVPLNMTDQKLLQASSQFQKKQGKGTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMIEPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLI 1145 HSP 2 Score: 103.605 bits (257), Expect = 4.618e-21 Identity = 66/210 (31.43%), Postives = 106/210 (50.48%), Query Frame = 0
Query: 899 DIAKKASDPI-KDEFKTILTQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL---------------------TQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
D K ++ P+ K E K N++D+++L + S+ K+ G IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF + K + +D R+MA LL+KFRIDF D+ ++ D+ K S F+ ++ I+D E+ KT R +RL ELLRE+S + +V++
Sbjct: 959 DDGKASTQPLLKKEVKDPSVPLNMTDQKLLQ-------------------ASSQFQKKQGKGTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMIEPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLIVMS 1148
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709292|sp|P55011.1|S12A2_HUMAN (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1) HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0 Identity = 380/992 (38.31%), Postives = 570/992 (57.46%), Query Frame = 0
Query: 32 ENNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPS-PKTPKGK-VIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASG-IEAEYLAYLGNVTS---KSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQM--SSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQV--------DLGVLEDIAEAIDEEEEEEEEKKKS-----KSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQA---------------------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
+ + NTY ++F TM+A+P HYR A+ + + RP++ EL E F E D T + K V+KFGWI GVL +LVI + +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGGAIG++F AN++AVA Y++GF ++++++L E+ + + +ND+R IGA+T+ +L ++V GMEW + + +LL++L+++ DFVIGTF+P +S+ GF GY +E F +N D++ + FF V A+FFPA TGI+AGAN+SGDL DP AIPKGTL A+++T Y+ A +GSC + +A+G + + L N TS K F +C C YG N+ Q +S +S L+ AG F+ATLSSA+ASLV AP++ QAL KDN+YP +F KG+G NN+PLRGY++ F IA I+I L+V++ ++SNFF+A YALINFSVFHAS+ KSPGWRP+FKYYN W+SL ILC VMF+++ A++T+V + LY++++Y+KP+ NWGSS A ++ +L++ L+ V+ HVKN+RP L+++G P R L+ + TK + L+IC HV Q M E + + WL +++KAF++ H+ + EGA + GLG++KPN +++G+K DW++ D + + H +FDI V ++RL+ G D S+ E L+ + D V DL + ++E + EEE+ K + K + P L +D KL++ F+ K+ IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF + K + +D R+MA LL+KFRIDF D++++ DI K F+ I+ I+D E+ KT R +RL ELL+EHSS + +
Sbjct: 202 DTHTNTYYL---RTFGHNTMDAVPRIDHYRHTAAQLGE----KLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEH-------SILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGP----DFREEET---FFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVAD-----QKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANII 1166 HSP 2 Score: 105.916 bits (263), Expect = 9.026e-22 Identity = 68/210 (32.38%), Postives = 108/210 (51.43%), Query Frame = 0
Query: 901 AKKASDPI-KDEFKTILTQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVTKKRVG--FIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQA---------------------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
K A+ P+ K E K + N++D+++L ++ T +K+ G IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF + K + +D R+MA LL+KFRIDF D++++ DI K F+ I+ I+D E+ KT R +RL ELL+EHSS + +V++
Sbjct: 982 GKTATQPLLKKESKGPIVPLNVADQKLL---------------------EASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMS 1169
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|341941994|sp|P55012.2|S12A2_MOUSE (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1) HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0 Identity = 382/991 (38.55%), Postives = 578/991 (58.32%), Query Frame = 0
Query: 32 ENNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPS-PKTPKGK-VIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASG-IEAEYLAYLGNVTS---KSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQM--SSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEE-----EEEEEKKKSKSNKRVPKTVLLGSDGK----PL---PAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKAS-----------DPIK----DEFKTILTQAN------ISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
+ + NTY ++F TM+A+P HYR A+ + + RP++ EL E F E D T + K V+KFGWI GVL ++VI++ +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGGAIG++F AN++AVA Y++GF ++++++L E+ + + +ND+R IGA+T+ +L ++V GMEW + + +LL++L+++ DFVIGTF+ +S+ GF GY +E F +N D++ + FF V A+FFPA TGI+AGAN+SGDL DP AIPKGTL A+++T YI A +GSC + +A+G + L N TS K F C C YG N+ Q +S +S L+ AG F+ATLSSA+ASLV AP++ QAL KDN+YP +F KG+G NN+PLRGY++ F IA I+I L+V++ ++SNFF+A YALINFSVFHAS+ KSPGWRP+FKYYN W+SL ILC VMF+++ A++T+V + LY++++Y+KP+ NWGSS A ++++L++ L+ V+ HVKN+RP L+++G P R L+ + TK + L+IC HV Q M E + + WL +++KAF++ H+ + EGA + GLG++KPN +++G+K DW++ D + + H +FDI V ++RL+ G D S+ E L+ S SP + D+ V D ++ D++ E+ +K + + + P LL + K PL KL++ F+ K+ IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF + K + +D R+MA LL+KFRIDF D++++ DI K +P + D+ + I + I+D E+ KT R +RL ELL+EHSS + +
Sbjct: 195 DTHTNTYYL---RTFGHNTMDAVPRIDHYRHTAAQLGE----KLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEH-------SILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGP----DFREEET---FFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELLS---SQEKSPGTK-DVVVNVDYSKKSDQDTCKSSGEKSITQKDEEEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANII 1159 HSP 2 Score: 103.605 bits (257), Expect = 4.344e-21 Identity = 68/210 (32.38%), Postives = 111/210 (52.86%), Query Frame = 0
Query: 901 AKKASDPI-KDEFKTILTQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVTKKRVG--FIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKAS-----------DPIK----DEFKTILTQAN------ISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
K + P+ K E K + N++D+++L ++ T +K+ G IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF + K + +D R+MA LL+KFRIDF D++++ DI K +P + D+ + I + I+D E+ KT R +RL ELL+EHSS + +V++
Sbjct: 975 GKTPTQPLLKKESKGPIVPLNVADQKLL---------------------EASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMS 1162
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|2500792|sp|Q25479.1|NKCL_MANSE (RecName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter; AltName: Full=Na-K-CL symporter) HSP 1 Score: 631.328 bits (1627), Expect = 0.000e+0 Identity = 378/1023 (36.95%), Postives = 575/1023 (56.21%), Query Frame = 0
Query: 5 SLDNVAFQKSEKGEPPNGGFYQSTSISENNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKV-IKFGWIDGV---------------------------LGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAK--SEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRE-CQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDPAG-FESLAKVIHHSFDINMAVAILRLQNGCDYSN-----------------------KIKSEHLVKIPFSDADSPDAQVD------LGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ-------------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
S++NV E+G PN S SE + + +S +LT EALP +YR + + +RP++GEL H + E K+ K+P V IK GWI GV L L++I++ +V +TTLSMSA+ TNG ++GGGIYY+ISRSLG EFG ++GI+F AN++A + IGF DSL D+L N G+ T D +ND+R +G V L + I+ +GM+W ++ + L+ +++ + VDFV+GT + K SE GFVG ++ TF +N + K +N FF V A+FFP+VTGI AGAN+SGDL+DPA AIPKGTL A++++ SY + G L +ASG + L G VT S + C + C+YG N + +S G +Y GC+AATLS+A+ +L+ PR++QAL D +YP L F K +G + +P RGYV+ FF++ ++I +L+ ++ L+SNF++A YALINF FH ++ + GWRP+F+YYN W+SL ++CVA+M L+ V +++TF LYL + YR+P+ NWGSS AQ + T+L + AL +HVKNY P +L+L+G P R LVD NL++K SL+I + +E + K+ + + WL +++AF S H +F GA + + +G+G+L PN+++MGYKSDW PA S V+H +F+ +AVAI+R+ G DYS +++ + L+ SD D D Q L + + I E +E++++KK+ K N + V + G L + +++ F+ K+ G +DVWWLYDDGGL++LLP+I+++R+ +A+C +R+F+LA + ++++ R+MANLLAKFRID+ + M+ DI +DP + E K + + ISD E+ + KTNR LRL ELL +S D+ V
Sbjct: 35 SVENV-----ERGVAPN-------SQSEGWHHESGWKRRRSLAQLTREALPRMENYRN--------SKRALKRPSLGELHGDH--------LITEEDEKDQNHRDTKSPTPAVGIKLGWIQGVFIPCLLNIWGVMLFLRLSWVVSQAGIGLSLVIIAISAIVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFGASVGIIFAFANAVAASMNTIGFCDSLNDLLRSN-----GLKITEDP-INDVRIVGTVALLVMCIICAIGMDWESKAQNFLIAIIVGAMVDFVVGTIMGPKDNSEIAKGFVGLSSATFVENFKSDFRFSEKLDQN---FFSVFAIFFPSVTGIQAGANISGDLKDPASAIPKGTLLALLISMVSYTLMVLFAGGGALRDASGNITDLLIVNGTVTDYSSVSL--CALNNTCEYGLHNSYSVMQLMSAWGPFIYGGCWAATLSTALTNLLSVPRLIQALGVDRIYPGLIFFSKPYGRHGEPYRGYVLTFFVSLLFLLIADLNTIAPLISNFYLASYALINFCTFHRALVRPLGWRPTFRYYNMWLSLAGFLMCVAIMLLVHWVMSLVTFAIFFTLYLIVHYRRPDVNWGSSTQAQMYKTALSSAHALARTGEHVKNYWPQLLVLAGRPQARPALVDLGNLISKAGSLMIVGDISQEKLSYKVRSARARSDDEWLRGRKVRAFCSRVHGFSFEPGARALVQGSGVGRLAPNVLLMGYKSDWTTCPANDLVSYFNVLHTAFENRLAVAIVRVSGGLDYSAVVSEGAEEGAAGSLTATSSSGELRVRRDGLIMHADSDLDIRDTQPKHNLSNLLTLTTSRSFTISECKEKDKKKKERKPNDMHRQIVYNTASGLELSKFQLAQMSLFQKKQESGTLDVWWLYDDGGLTILLPYIISQRSAWANCKLRIFALANRLHEMELEERNMANLLAKFRIDYSSLTMVQDI----TDPPQPETKALFDETIKKFTEESASPDCRISDMELQTLAVKTNRQLRLRELLLANSKDARLV 1014 HSP 2 Score: 108.227 bits (269), Expect = 1.672e-22 Identity = 61/145 (42.07%), Postives = 94/145 (64.83%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ-------------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
K+ G +DVWWLYDDGGL++LLP+I+++R+ +A+C +R+F+LA + ++++ R+MANLLAKFRID+ + M+ DI +DP + E K + + ISD E+ + KTNR LRL ELL +S D+ VV++
Sbjct: 877 KQESGTLDVWWLYDDGGLTILLPYIISQRSAWANCKLRIFALANRLHEMELEERNMANLLAKFRIDYSSLTMVQDI----TDPPQPETKALFDETIKKFTEESASPDCRISDMELQTLAVKTNRQLRLRELLLANSKDARLVVMS 1017
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709297|sp|P55016.1|S12A1_RAT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 626.32 bits (1614), Expect = 0.000e+0 Identity = 380/1011 (37.59%), Postives = 570/1011 (56.38%), Query Frame = 0
Query: 24 FYQSTSISENNMNTYVYDAD------QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGEL----------ASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVLGLLVISVCNL---------------------------VTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK-YGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKS----GLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMA-EQMSSHI---EHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDP-AGFESLAKVIHHSFDINMAVAILRLQNGCDYS---------NKIKSEHL-VKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHS 947
F Q+ ++ + + YD+ Q+F TM+A+P +YR S+ N RP++ E+ A G + M G E + + G V+KFGW+ GVL ++++ + VT LT +SMSA+ TNGV+RGGG YY+ISRSLG EFGG+IG++F AN++ VA Y++GF ++++D+L E+ D + VD ND+R IG++T+ +L ++V GMEW + + +LL++L++ +F IGT +P+ +E K GF Y F +N + GFF V A+FFPA TGI+AGAN+SGDL DP AIP+GT+ A+ +T +YI A + +C + +A+G + + N + G F C CQYG N+ Q +S +S G L+ AG F+ATLSSA+ASLV AP+V QAL KDN++ L F KG+G NN+PLRGY + F IA A I+I L+V++ ++SNFF+A YALINFS FHAS KSPGWRP++ YN WVSLF ILC AVMF+++ AVIT+V LY++++Y+KP+ NWGSS A +V++L N L TV+ HVKN+RP ++L+G P R L+D + TK L IC V P+K+ ++M+S + + WL ++IKAF++ + F +G S + +GLG++KPN +++GYK +W + P + E+ +IH +FD + V I+R+ G D S K++ E L ++ D D + + + L A ++ + K SN +++ +G KLV+ FK K+ G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ + K + ++ + SMA+LL+KFRI F D+ +I DI K P K+ +K LT A I+D E+ + EK+ R +RL ELL+EHS
Sbjct: 69 FLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVN----RPSLQEIHEQLAKNVAVAPGSADRVANGDGM---PGDEQAENKEEDVTG-VVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFRFANAVRVAMYVVGFAETVVDLLKES----DSM--MVDPT-NDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIGIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTEG-------EGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVRACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVG--PRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLN----ITKPAPKKDSNISTIQSMHVGE----FNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIK---PNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHS 1043 HSP 2 Score: 101.293 bits (251), Expect = 1.978e-20 Identity = 60/156 (38.46%), Postives = 91/156 (58.33%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
K+ G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ + K + ++ + SMA+LL+KFRI F D+ +I DI K P K+ +K LT A I+D E+ + EK+ R +RL ELL+EHS + +V++
Sbjct: 901 KQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIK---PNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLS 1052
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|212276464|sp|Q13621.2|S12A1_HUMAN (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 616.305 bits (1588), Expect = 0.000e+0 Identity = 383/1020 (37.55%), Postives = 575/1020 (56.37%), Query Frame = 0
Query: 24 FYQSTSISENNMNTYVYDAD------QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFM-----EVESGKEV----EDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK-YGFVGYNTETFTKNLLTTNYHDYKNPK--NPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKS----GLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMA-EQMSSHI---EHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDP-AGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEE------KKKSKSN--KRVPKTVLLGSDGK----------PLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHS 947
F QS ++ + + + YD+ Q+F TM+A+P +YR SI N RP++ E+ H K+ + V +G + + + + + V+KFGW+ GVL G+L+I + +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGG+IG++F AN++AVA Y++GF ++++D+L E+ D + VD ND+R IG++T+ +L ++V GMEW + + +LL++L+++ +F IGT +P+ +E K GF Y F +N P+ GFF V A+FFPA TGI+AGAN+SGDL DP AIP+GT+ A+ +T +Y+ A +G+C + +A+G + + N + G F C CQYG N+ Q +S +S G L+ AG F+ATLSSA+ASLV AP+V QAL KDN+Y L F KG+G NN+PLRGY++ F IA A I+I L+ ++ ++SNFF+A YALINFS FHAS KSPGWRP++ YN WVSLF +LC AVMF+++ AVIT+V LY++++ +KP+ NWGSS A +V++L N L TV+ HVKN+RP ++L+G P R L+D + TK L IC V P+K+ ++M+S + + WL ++IKAF++ + F +G S + +GLG++KPN +++GYK +W + P E+ +IH +FD + V I+R+ G D S ++ + ++ + + +A + D E EEE KK K N K PK DG KLV+ FK K+ G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ + K + ++ + MA+LL+KFRI F D+ +I DI + P K+ +K LT A I+D E+ + EK+ R +RL ELL+EHS
Sbjct: 73 FLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVN----RPSLLEI---HEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKES----DSM--MVDPT-NDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENF---------GPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVG--PRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIK-----------DNECEEESGGIRGLFKKAGKLNITKTTPK-----KDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIR---PNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHS 1047 HSP 2 Score: 99.3673 bits (246), Expect = 9.919e-20 Identity = 62/179 (34.64%), Postives = 100/179 (55.87%), Query Frame = 0
Query: 933 TNRHLRLAELLREHSSDSETVTKKR-VGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
T++ + + E ++ S KK+ G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ + K + ++ + MA+LL+KFRI F D+ +I DI + P K+ +K LT A I+D E+ + EK+ R +RL ELL+EHS + +V++
Sbjct: 882 TSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIR---PNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLS 1056
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|27151793|sp|P55014.2|S12A1_MOUSE (RecName: Full=Solute carrier family 12 member 1; AltName: Full=BSC1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 614.379 bits (1583), Expect = 0.000e+0 Identity = 387/1023 (37.83%), Postives = 572/1023 (55.91%), Query Frame = 0
Query: 24 FYQSTSISENNMNTYVYDAD------QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVED---PSPKTPKGK------VIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK-YGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKS----GLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMA-EQMSSHI---EHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDP-AGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLED----IAEAIDEEEEEEEE-------KKKSKSN--KRVPKTVLLGSDGK----------PLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHS 947
F Q+ ++ + + YD+ Q+F TM+A+P +YR S+ N RP++ E+ H K+ + + + P + + K V+KFGW+ GVL G+L+I + +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGG+IG++F AN++AVA Y++GF ++++D+L E+ D + VD ND+R IG++T+ +L ++V GMEW + + +LL++L+++ +F IGT +P+ +E K GF Y F +N + GFF V A+FFPA TGI+AGAN+SGDL DP AIP+GT+ A+ +T +YI A + +C + +A+G + + N + G F C CQYG N+ Q +S +S G L+ AG F+ATLSSA+ASLV AP+V QAL KDN++ L F KG+G NN+PLRGY + F IA A I+I L+V++ ++SNFF+A YALINFS FHAS KSPGWRP++ YN WVSLF ILC AVMF+++ AVIT+V LY++++Y+KP+ NWGSS A +V++L N L TV+ HVKN+RP ++L+G P R L+D + TK L IC V P+K+ ++M+S + + WL ++IKAF++ + F +G S + +GLG++KPN +++GYK +W + P + E+ +IH +FD + V I+R+ G D S ++ Q +L LE + AI + E EE + KK K N K PK DG KLV+ FK K+ G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ + K ++ + SMA+LL+KFRI F D+ +I DI K P K+ +K LT A I+D E+ + EK+ R +RL ELL+EHS
Sbjct: 69 FLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVN----RPSLLEI---HEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKES----DSM--MVDPT-NDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKG-------EGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVG--PRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQ----------------VQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPK-----KDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIK---PNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHS 1043 HSP 2 Score: 100.523 bits (249), Expect = 3.412e-20 Identity = 60/156 (38.46%), Postives = 90/156 (57.69%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
K+ G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ + K ++ + SMA+LL+KFRI F D+ +I DI K P K+ +K LT A I+D E+ + EK+ R +RL ELL+EHS + +V++
Sbjct: 901 KQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIK---PNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLS 1052
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709296|sp|P55015.1|S12A1_RABIT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0 Identity = 377/1013 (37.22%), Postives = 569/1013 (56.17%), Query Frame = 0
Query: 24 FYQSTSISENNMNTYVYDAD------QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFM----------EVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK-YGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYL--AYLGNVTSKSGLFFD--NCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMA-EQMSSHI---EHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDP-AGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEE--------KKKSKSN--KRVPK-----TVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL---------------------TQANISDEEVLSNLEKTNRHLRLAELLREHS 947
F Q+ ++ + + + YD+ Q+F TM+A+P +YR S+ N RP++ E+ H K+ + E G+E + + K +KFGW+ GVL G+++I + +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGG+IG++F AN++AVA Y++GF ++++D+L E+ D + VD ND+R IG++T+ +L ++V GMEW + + +LL++L+++ +F IGT +P+ +E K GF Y F +N + GFF V A+FFPA TGI+AGAN+SGDL DP AIP+GT+ A+ +T +YI A +G+C + +A+G + + N ++ GL +D C CQYG N+ Q +S +S G L+ AG F+ATLSSA+ASLV A +V QAL KDN+Y L F KG+G NN+PLRGY++ F IA A I+I L+ ++ ++SNFF+A YALINFS FHAS KSPGWRP++ YN WVSLF +LC AVMF+++ AVIT+V LY++++Y+KP+ NWGSS A +V++L N L TV+ HVKN+RP +L+G P R L+D TK L IC V P+K+ ++M+S + + WL ++IKAF++ + F +G S + +GLG++KPN +++GYK W + P E+ +IH +FD + V I+R+ G D S ++++ ++ L EA ++ E EE KK K N K PK + KLV+ FK K+ G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ + K + ++ + +MA+LL+KFRI F D+ +I DI K + F+ ++ T I+D E+ + EK+ R +RL ELL+EHS
Sbjct: 72 FLQTGETTKTDASFHTYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVN----RPSLLEI---HEQLAKNVAVTPGSADRVANGEGMPGEEHAENKEEDNKAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVVIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKES----DSM--MVDPT-NDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKG-------EGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGINCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAAKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCFVLTGGPMTRPALLDITYAFTKNSGLCICCEVFVG--PRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKKWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDIS------QVLQVQEELEKLEQERLAL-------EATIKDNECEEGNGGIRGLFKKVGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINIKPNKESWKFFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHS 1047 HSP 2 Score: 99.7525 bits (247), Expect = 7.425e-20 Identity = 55/153 (35.95%), Postives = 89/153 (58.17%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL---------------------TQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
K+ G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ + K + ++ + +MA+LL+KFRI F D+ +I DI K + F+ ++ T I+D E+ + EK+ R +RL ELL+EHS + +V++
Sbjct: 905 KQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINIKPNKESWKFFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLS 1056
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|313104194|sp|P55017.3|S12A3_HUMAN (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl cotransporter; Short=NCC; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter) HSP 1 Score: 593.193 bits (1528), Expect = 0.000e+0 Identity = 353/1029 (34.31%), Postives = 562/1029 (54.62%), Query Frame = 0
Query: 1 MCTDSLDNVAFQKSEKGEPPNGGFYQSTSISE-NNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHA---------------SFMEVESGKEVEDPSPKTPKGKVIKFGWIDGV---------------------------LGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSE-NVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLP-AKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYL----AYLGNVTSKSGLFFDNCTMRE-CQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAE--QMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKI--PFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAK-----------------KASDPIKDEFKTILTQAN----ISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
+C+ S++ PP Y S+ S + +T+ ++F T++ +P+ HY + P RPT+ +L S + E +H +E E+G E +P+ P ++FGW+ GV L ++I + VT +T LS+SA+STNG ++ GG Y++ISRSLG E GG+IG++F AN++ VA + +GF +++ D+L E P D I ND+R I V++T +L +++ GMEW ++ + L L+++VS ++++GT +P ++ +A GF Y + F +NL+ D++ P FF + ++FFP+ TGI+AGAN+SGDL+DPA+AIPKGTL A+ T SY+ + IGSC + +ASG+ + + + G F CT + C YG N QT+S +S L+ AG F ATLSSA+A LV A +V Q L +D LYP +G FGKG+G N +P+RGY++ + IA A I+I L+ ++ ++SNFF+ YALINFS FHASIT SPGWRPSF+YYNKW +LF I+ V +MFL++ A+I + L L++ Y+KPE NWGSS A + +L LN V+ H+KNYRP L+L+G P R LVDF T+ LSL+IC HV Q+M E +++ WL+ +IKAF+S +++ G + GLG++KPN++++G+K +W PA E ++H +FD N V ++R++ G + S K+ H+ + P D A+VD +A+ +EE Q F++++ ID++WL+DDGGL+LL+P++L ++ +++ C +RVF + + + +D + +++ +LL+KFR+ FH+V ++PDI + + +D KDE + + ISDEE+ N K+ R +RL E++ ++S D+ +
Sbjct: 15 LCSGRFTISTLLSSDEPSPPAA--YDSSHPSHLTHSSTFCM---RTFGYNTIDVVPTYEHYAN----STQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEK-NPEEP----VRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPI--------NDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVP----DWRGPDGT--FFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVS-KMMQAHINPVFDPAEDGKEASARVD-------PKALVKEE---------------------------------QATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVF-VGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALI 973 HSP 2 Score: 94.3597 bits (233), Expect = 3.193e-18 Identity = 50/165 (30.30%), Postives = 98/165 (59.39%), Query Frame = 0
Query: 942 LLREHSSDSETVTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAK-----------------KASDPIKDEFKTILTQAN----ISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
L++E + + +++ ID++WL+DDGGL+LL+P++L ++ +++ C +RVF + + + +D + +++ +LL+KFR+ FH+V ++PDI + + +D KDE + + ISDEE+ N K+ R +RL E++ ++S D+ +VI
Sbjct: 812 LVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVF-VGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVI 975
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|27151789|sp|P55018.2|S12A3_RAT (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter) HSP 1 Score: 592.423 bits (1526), Expect = 0.000e+0 Identity = 350/979 (35.75%), Postives = 545/979 (55.67%), Query Frame = 0
Query: 44 QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKH--ASFMEVESGKEVEDP---------SPKTPKGKVIKFGWIDGV---------------------------LGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSEN-VPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPA-KSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYL----AYLGNVTSKSGLFFDNCTMRE-CQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAE--QMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKK-------------ASDPIKDEFKTILTQA--------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
++F T++ +P+ HY A P RPT+ +L S + E H A + G E+ D S K+P G+ ++FGW+ GV L L+I + +VT +T LS+SA+STNG ++ GG Y++ISRSLG E GG+IG++F AN++ VA + +GF +++ D+L EN P D I ND+R IG VT+T +L +++ GMEW ++ + L L+++VS ++++GT +PA K +A GF Y+ + F +NL+ D++ FF + ++FFP+ TGI+AGAN+SGDL+DPA+AIPKGTL A+ T SY+ + IGSC + +ASG + + + G F C+ + C+YG N QT+S +S L+ AG F ATLSSA+A LV A +V Q L +D LYP +G FGKG+G N +P+RGY++ + IA A I+I L+ ++ ++SNFF+ YALINFS FHASIT SPGWRPSF+YY+KW +LF ++ V +MFL++ A+I + L L++ Y+KPE NWGSS A + +L LN V+ H+KNYRP L+L+G P R LVDF + T+ LSL+IC HV Q++ E ++S WL+ +IKAF+S +++ G + +GLG++KPN++++G+K +W PA E ++H +FD N V I+R++ G + S +++ +P+A V +EE+ +S+ K+ ID++WL+DDGGL+LL+P++L ++ ++ C +RVF + + + +D + +++ +LL+KFR+ FH+V ++PDI +K A + D FK T A ISDEE+ N K+ R +RL E+L ++S D+ +
Sbjct: 52 RTFGYNTIDVVPAYEHYANSAL----PGEPRKVRPTLADLHSFLKQEGSHLHALAFDGRPGHELTDGLVEDETGANSEKSP-GEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVDPI--------NDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASKDKASKGFYSYHGDIFVQNLVP----DWRGIDG--SFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTITPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIAPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNVSEALQTH----------TAPEALV--------------QEEQTSTIFQSEQGKKT--------------------------------IDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVF-VGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALI 954 HSP 2 Score: 96.6709 bits (239), Expect = 5.307e-19 Identity = 56/183 (30.60%), Postives = 102/183 (55.74%), Query Frame = 0
Query: 924 EEVLSNLEKTNRHLRLAELLREHSSDSETVTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKK-------------ASDPIKDEFKTILTQA--------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
E L+ E H L++E + + +++ ID++WL+DDGGL+LL+P++L ++ ++ C +RVF + + + +D + +++ +LL+KFR+ FH+V ++PDI +K A + D FK T A ISDEE+ N K+ R +RL E+L ++S D+ +++
Sbjct: 775 REGLNVSEALQTHTAPEALVQEEQTSTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVF-VGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALIIL 956
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1325281518|ref|XP_023325442.1| (solute carrier family 12 member 2-like [Eurytemora affinis]) HSP 1 Score: 892.493 bits (2305), Expect = 0.000e+0 Identity = 486/945 (51.43%), Postives = 640/945 (67.72%), Query Frame = 0
Query: 67 MSDPTNSST-ERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADT-VDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKY--GFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGN-VTSKSGLF---FDNCTM--------RECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLV------------KIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---LTQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
MS NS +RPTI EL ++ + + +VE K KGKVIKFGWI+GV GLLV++ NLVT +T +SMSAV+TNG I+GGGIY+MISR+LG EFGGAIG+MFT+ANSIAV+ YIIGF +SLLDM + +PG+ GI D ++RLND+R IG VTL +L+LA VGM+WVTRV+K LLLLL+ SQVDF+IGTF+P KS+ + GF G++ ET TKN+ +Y + F V AVFFPAVTGIVAGANLSGDL+DPA AIPKGTL A+ TY +YI+Y ++ Y SG+ EY + + + + + +D+C+ EC++G+ NDQQT++ IS TGYL+Y GCFAATLSSAIASLVGAPRVLQALAKD LYP + F GFGANNDP+RGY++VF I+ C++IG L++VSSLLSNFFVA YALINFSVFHASITKSPGWRP+FKYY+ WVSL T+LCV VMF+M VTA++T+V + +LY FI YRKPEANWGSS AQ FV++L+ +Q+L Q HVKNYRP +L+L+G PAHR PLVDFANL+TKKLSLLICAHV E + +A M + WL+DH+IK F+++T S ++ EGA + + L GLGKL PNLV++G+KSDWM + G V+H ++D +++ ILR++NG D+S I +E V + + + + D ++I E + E + ++++++ RV DG PL ++ I F+ +K+ GFIDVWWLYDDGGL+LL+P+IL+ R + DC +RVF+L K D LD +R+M LLAKFRIDF DV++IPD+ K+A D K+E K I L + IS++++L N EKTNRHLRLAELLREHS +S+ +
Sbjct: 1 MSFGRNSGKGQRPTIEELRDEEYSKPEQSGAGDVEGQK----------KGKVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQCGIVQGLLVMTTANLVTLVTAISMSAVATNGQIKGGGIYFMISRALGPEFGGAIGLMFTLANSIAVSMYIIGFAESLLDMFQDYIPGWSGIVDADFENRLNDVRIIGVVTLVLILVLAFVGMDWVTRVQKGLLLLLLCSQVDFLIGTFIP-KSDLDHSKGFTGWSLETVTKNV-QPHYEEQSF-------FSVFAVFFPAVTGIVAGANLSGDLKDPATAIPKGTLLAIGTTYLTYILYGVVVAFTYSPYGSGVAEEYSIWTNDSIPLEEKMLVPRWDDCSADATLLRNGTECRFGTLNDQQTMAVISFTGYLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPKIEFFAPGFGANNDPIRGYILVFLISLGCVLIGELNMVSSLLSNFFVAAYALINFSVFHASITKSPGWRPAFKYYSPWVSLLGTLLCVCVMFMMDWVTALLTYVIVIVLYFFIYYRKPEANWGSSAQAQVFVSALKGVQSLTETQDHVKNYRPKVLVLTGNPAHRPPLVDFANLITKKLSLLICAHVITEPGSKHLA-LMRESTQAWLNDHKIKGFYTVTESGSWEEGAKACLTLAGLGKLAPNLVLIGFKSDWMTNLEGLHQYFNVLHSAYDHALSIGILRVKNGLDFSEHILAEETVGDHSEERRGSGDEEKEKEKKEDEEEGDGEDKDEIGELQSKTEVVQAVRQRTRTISRVTYET---HDGTPLDKTIIDSIQQFQGEKKKGFIDVWWLYDDGGLTLLIPYILSTRKMYTDCSLRVFTLGNKKDDLDRDTRNMCTLLAKFRIDFSDVIVIPDVTKRAQDATKEELKQILDKLPEDCISEDDLLMNKEKTNRHLRLAELLREHSKESQQI 922 HSP 2 Score: 171.785 bits (434), Expect = 6.932e-40 Identity = 78/134 (58.21%), Postives = 103/134 (76.87%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---LTQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
+K+ GFIDVWWLYDDGGL+LL+P+IL+ R + DC +RVF+L K D LD +R+M LLAKFRIDF DV++IPD+ K+A D K+E K I L + IS++++L N EKTNRHLRLAELLREHS +S+ +V+
Sbjct: 791 EKKKGFIDVWWLYDDGGLTLLIPYILSTRKMYTDCSLRVFTLGNKKDDLDRDTRNMCTLLAKFRIDFSDVIVIPDVTKRAQDATKEELKQILDKLPEDCISEDDLLMNKEKTNRHLRLAELLREHSKESQQIVM 924
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1228359575|ref|XP_021952830.1| (solute carrier family 12 member 3-like [Folsomia candida]) HSP 1 Score: 889.412 bits (2297), Expect = 0.000e+0 Identity = 472/992 (47.58%), Postives = 631/992 (63.61%), Query Frame = 0
Query: 34 NMNTYVYDA--DQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFL-PAKS-EAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEY-LAYLGNVT---SKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLV------------KIPFSDADSPDAQ---------------VDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ---------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
N TYVYD +S +LT EALP+ +YR L S+ + P RPT+ EL + TE A M+ E+G+ + KGKVIKFGW++GVL G L+IS+ NLVTF+T SMSAVSTNG I+GGGIYYMISRSLG EFG AIG+MFT+ANSIAV+ YI+GF +SL D+L N G +D ND+R IG L +L+LA VGM+WVTR + LL++LI +QV+F+IG+F+ P E GF GYN ETF N + Y F+ V VFFPAVTGIVAGANLSGDL+DPA AIPKGTL A+ +TY SYI YA ++G C + EASG EY L +T ++ + +DNCT R+C+YG N Q + IS G L+YAGCFAATLSSAIASLVGAPRV QAL KD LYP + F KG+GANNDP+RGY +VF I+ C++I L+ ++ LLSNFF+ Y LINFSVFHASI+ SPGWRP+FKYYN WVSL +LC+AVMFL++ TA+ T C+ LYL++SYRKPE NWGSS AQ F ++L+++Q LN V++HVKNYRP IL+++G+P +R PL+DFA L+ K LSL++C HV K QK+ ++ WL H+ K F+++ ++F GA S + + GLGKL+PN+V+MGYK+DW++ + + VIH + D+++AV ILR G DYSN I+ E + +IP + ++S +Q V+ L D + ++ + KKS+ K + G G LP ++ I F+ K++ G IDVWWLYDDGGLS+LLP++LT R Q+A C +R+FSL ++ D L + R+MA LL+KFRID+ DV++IPDI KKA D K EF++++ + +I++ E++S+ EKTNRHLRL ELL+ HS DS V
Sbjct: 57 NQYTYVYDTRYHKSLGQLTREALPALDNYRDLLSVHAAP------RPTLDELHNATYTEKTKA--MDEETGEVL--------KGKVIKFGWLEGVLMRCMLNIWGVMLFLRLSWCVGQAGILEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTIANSIAVSLYIVGFCESLNDLLKANNWG-----QLIDGGSNDIRVIGVGVLVAILVLAFVGMDWVTRTQMFLLVVLIAAQVNFIIGSFIGPVDDLEKAKGFPGYNAETFVTNFWSDYRTAYGEDTTKQDFWTVFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYLSYIGYAVMMGGCAMREASGNLTEYNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVIEEEGQLTPQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPD--QVVNNQLVGFSSNKKSR--KHSLSAMYKGPGGAILPRHVLSNITQFQRKQKKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVIAEFKSDDNQDGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFV 1023 HSP 2 Score: 160.614 bits (405), Expect = 3.308e-36 Identity = 74/140 (52.86%), Postives = 103/140 (73.57%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ---------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
K++ G IDVWWLYDDGGLS+LLP++LT R Q+A C +R+FSL ++ D L + R+MA LL+KFRID+ DV++IPDI KKA D K EF++++ + +I++ E++S+ EKTNRHLRL ELL+ HS DS VV+
Sbjct: 886 KQKKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVIAEFKSDDNQDGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFVVM 1025
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1215275570|gb|OXA61879.1| (Bumetanide-sensitive sodium-(potassium)-chloride cotransporter [Folsomia candida]) HSP 1 Score: 885.174 bits (2286), Expect = 0.000e+0 Identity = 470/1001 (46.95%), Postives = 631/1001 (63.04%), Query Frame = 0
Query: 34 NMNTYVYDA--DQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL------------------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFL-PAKS-EAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEY----LAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLV------------KIPFSDADSPDAQ---------------VDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ---------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
N TYVYD +S +LT EALP+ +YR L S+ + P RPT+ EL + TE A M+ E+G+ + KGKVIKFGW++GVL G L+IS+ NLVTF+T SMSAVSTNG I+GGGIYYMISRSLG EFG AIG+MFT+ANSIAV+ YI+GF +SL D+L N G +D ND+R IG L +L+LA VGM+WVTR + LL++LI +QV+F+IG+F+ P E GF GYN ETF N + Y F+ V VFFPAVTGIVAGANLSGDL+DPA AIPKGTL A+ +TY SYI YA ++G C + EASG EY + + + ++ + +DNCT R+C+YG N Q + IS G L+YAGCFAATLSSAIASLVGAPRV QAL KD LYP + F KG+GANNDP+RGY +VF I+ C++I L+ ++ LLSNFF+ Y LINFSVFHASI+ SPGWRP+FKYYN WVSL +LC+AVMFL++ TA+ T C+ LYL++SYRKPE NWGSS AQ F ++L+++Q LN V++HVKNYRP IL+++G+P +R PL+DFA L+ K LSL++C HV K QK+ ++ WL H+ K F+++ ++F GA S + + GLGKL+PN+V+MGYK+DW++ + + VIH + D+++AV ILR G DYSN I+ E + +IP + ++S +Q V+ L D + ++ + KKS+ K + G G LP ++ I F+ K++ G IDVWWLYDDGGLS+LLP++LT R Q+A C +R+FSL ++ D L + R+MA LL+KFRID+ DV++IPDI KKA D K EF++++ + +I++ E++S+ EKTNRHLRL ELL+ HS DS V
Sbjct: 57 NQYTYVYDTRYHKSLGQLTREALPALDNYRDLLSVHAAP------RPTLDELHNATYTEKTKA--MDEETGEVL--------KGKVIKFGWLEGVLMRCMLNIWQVNANFENEGVMLFLRLSWCVGQAGILEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTIANSIAVSLYIVGFCESLNDLLKANNWG-----QLIDGGSNDIRVIGVGVLVAILVLAFVGMDWVTRTQMFLLVVLIAAQVNFIIGSFIGPVDDLEKAKGFPGYNAETFVTNFWSDYRTAYGEDTTKQDFWTVFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYLSYIGYAVMMGGCAMREASGNLTEYNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVIEEEGQLTPQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPD--QVVNNQLVGFSSNKKSR--KHSLSAMYKGPGGAILPRHVLSNITQFQRKQKKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVIAEFKSDDNQDGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFV 1032 HSP 2 Score: 160.614 bits (405), Expect = 3.448e-36 Identity = 74/140 (52.86%), Postives = 103/140 (73.57%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ---------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
K++ G IDVWWLYDDGGLS+LLP++LT R Q+A C +R+FSL ++ D L + R+MA LL+KFRID+ DV++IPDI KKA D K EF++++ + +I++ E++S+ EKTNRHLRL ELL+ HS DS VV+
Sbjct: 895 KQKKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVIAEFKSDDNQDGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFVVM 1034
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1339058872|ref|XP_023717973.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Cryptotermes secundus]) HSP 1 Score: 881.322 bits (2276), Expect = 0.000e+0 Identity = 474/1017 (46.61%), Postives = 632/1017 (62.14%), Query Frame = 0
Query: 28 TSISENNMNTYVYDAD-QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAK--SEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQ----------------VDLGVLEDIAEA------------------IDEEEEEE------------EEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILT-----------------QANISDEEVLSNLEKTNRHLRLAELLREHSSDS 950
TS + N +YVYD+ +S+ +LT E P HYR D + RPT+ EL H + +S E + + + KGKVIKFGW++GV G +I + N+VT +TT+SMSAV TNG I+GGGIYYMISRSLG EFGGAIG+MFT+ANS+AV+ YI+GF +SL D+L F +D ND+R +G VTL +L+LA+VGM+WVTR + LL +LI SQ+DF++G+FL + E GFVGYN+ETF NL +Y +Y+ + FF V AVFFPAVTGIVAGANLSGDL+DP+ AIP GTL A+ +T+ SYI YA ++G C L ASG EYL G + F NCT R C YG ND Q + S G L+Y GCFAATLSSAIASLVGAPRVLQALAKD LYP++G F KG GANNDP+RGYV+VF I+F CI+I L+ ++ LLSNFF+A YALINFSVFHASI+KSPGWRP+FKYYN WVSL T+LC+AVMFLMS V A+ITFV + LYL++SYRKPE NWGSS A + ++L+ + LN V++HVKN+RP +L+L+G+P R PLVDFA L+TK LSLL+CA + K + Q++ + ++ HWL H++KAFF+I +F G+ + + ++GLGKL+PN+++MGYK +W D + VIH++ D +AV ILR+Q G DYS I+ E V P S D DA+ V++ VL D + D E+ ++ +K N + +G G+PLP ++ I F+ K+ G IDVWWLYDDGGL+LL+P+IL+ R+QF+ C +R+FSLA+K D LD R++A LL+KFRID+ DVV+I DI KKA + + EF+ + Q I+D E+++ EKTNRH+R+ ELL ++S +S
Sbjct: 48 TSSTPNQQYSYVYDSRYKSWGQLTREVPPRVEHYR-------DLSIQHQYRPTLDEL---------HERNIPEKSDGEDKVEAQQQKKGKVIKFGWLEGVFMRCLLNIWGVMLFLRLSWVVGQAGIGEGTAIICLANIVTLVTTISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRT----FG--TTLIDGASNDIRVVGLVTLIGILVLAIVGMDWVTRTQMCLLFVLIASQIDFMVGSFLGPQDDEEIAKGFVGYNSETFRSNL-GPDYRNYEGVDH--DFFSVFAVFFPAVTGIVAGANLSGDLKDPSSAIPVGTLLAIFVTFLSYIGYAFMMGGCVLRTASGNVTEYLQVKGEPDEWA--VFSNCTDRSCLYGLHNDSQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSKGHGANNDPVRGYVLVFVISFGCILIAQLNAIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLAGTLLCIAVMFLMSWVMALITFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCADILKGTHSQRVHDALTRKAYHWLIRHKVKAFFNIVEDDSFEAGSQALMQISGLGKLRPNMILMGYKGNWRTCDREELKQYFNVIHNALDRYLAVGILRVQEGLDYSGVIQDEEFV--PISKLDKKDAEEKMRRNESTNQLESQDVNITVLSDALSSDTDSPPVTPKMQHGGTVNCDPAATEKLLQSSTTVTITSKKMRKVSKNHKSSFPTYVGPGGEPLPKSILNNITQFQRKQPKGLIDVWWLYDDGGLALLIPYILSTRSQFSGCQLRIFSLASKRDDLDKDQRNLAALLSKFRIDYSDVVIISDIHKKAMESTRKEFEIFIQPFRVKDEENFETSKAIEQTYITDSELIALREKTNRHMRIRELLLQYSKES 1035 HSP 2 Score: 145.591 bits (366), Expect = 1.405e-31 Identity = 70/149 (46.98%), Postives = 100/149 (67.11%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILT-----------------QANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
K+ G IDVWWLYDDGGL+LL+P+IL+ R+QF+ C +R+FSLA+K D LD R++A LL+KFRID+ DVV+I DI KKA + + EF+ + Q I+D E+++ EKTNRH+R+ ELL ++S +S +V+A
Sbjct: 893 KQPKGLIDVWWLYDDGGLALLIPYILSTRSQFSGCQLRIFSLASKRDDLDKDQRNLAALLSKFRIDYSDVVIISDIHKKAMESTRKEFEIFIQPFRVKDEENFETSKAIEQTYITDSELIALREKTNRHMRIRELLLQYSKESTFIVMA 1041
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1228009301|ref|XP_021936563.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X2 [Zootermopsis nevadensis]) HSP 1 Score: 869.766 bits (2246), Expect = 0.000e+0 Identity = 477/1005 (47.46%), Postives = 627/1005 (62.39%), Query Frame = 0
Query: 31 SENNMNTYVYDAD-QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKY--GFVGYNTETFTKNLLTTNYHDYKNPKN-PPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQ----------------VDLGVLEDIAEAIDEE--------------EEEEEEKKKSKSNKRVPKT------VLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL-----------------TQANISDEEVLSNLEKTNRHLRLAELLREHSSDS 950
S N ++Y D+ +S +LT E P HYR L SI + RPT+ EL H + + + VE + KGKVIKFGW++GV G +I + N+VT +T++SMSAV TNG I+GGGIYYMISRSLG EFGGAIG+MFT+ANS+AV+ YI+GF +SL D+L F +D ND+R +G VTL + ILA+VGM+WVTR + LL +LI SQ+DFVIG+FL + + + GFVGYN ETF NL Y DY+N + FF V +VFFPAVTGIVAGANLSGDL+DP+ AIP GTL A+V+T+ SY+ Y ++G C L ASG EYL G + F NCT R C YG ND Q + S G L+Y GCFAATLSSAIASLVGAPRVLQALAKD LYP +G F KG GANNDP+RGY++VF I+F CI+I L+V++ LLSNFF+A YALINFSVFHASI+KSPGWRP+FKYYN WVSL T+LC+AVMFLMS V A+ TFV + LYL++SYRKPE NWGSS A + ++L+ + LN V++HVKN+RP +L+L+G+P R PLVDFA L+TK LSLL+CAH+ K + Q++ E ++ WL H++KAFF IT +F G + ++GLGKL+PN+++MGYK +W DP + VIH++ D +AV ILR+Q G DYS I+ E V P S D DA+ V++ VL D + E E SK +++ KT + + G+PLP ++ I F+ K+ G IDVWWLYDDGGL+LL+P+IL+ R+QF+ C +R+FSLA+K D LD R++A LL+KFRID+ DVV+I DI KKA D + EF+T + + I++ E+L+ EKTNRH+R+ ELL ++S +S
Sbjct: 46 SPNLQHSYGCDSRYKSLGQLTREVPPRVEHYRHL-SIHHE------YRPTLEEL---------HERIIPEKRERNVEAEPQEQKKGKVIKFGWLEGVFMRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRS----FG--TTIIDGASNDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNL----YPDYRNYEGVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTEYLQVKGEPNEWT--IFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRTCDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFV--PISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDPVASDSLLESSTTVTVTSKKSRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPKGLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMES 1020 HSP 2 Score: 145.976 bits (367), Expect = 1.019e-31 Identity = 71/149 (47.65%), Postives = 101/149 (67.79%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL-----------------TQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
K+ G IDVWWLYDDGGL+LL+P+IL+ R+QF+ C +R+FSLA+K D LD R++A LL+KFRID+ DVV+I DI KKA D + EF+T + + I++ E+L+ EKTNRH+R+ ELL ++S +S +V+A
Sbjct: 878 KQPKGLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMA 1026
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1228009299|ref|XP_021936562.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X1 [Zootermopsis nevadensis]) HSP 1 Score: 869.381 bits (2245), Expect = 0.000e+0 Identity = 477/1005 (47.46%), Postives = 627/1005 (62.39%), Query Frame = 0
Query: 31 SENNMNTYVYDAD-QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKY--GFVGYNTETFTKNLLTTNYHDYKNPKN-PPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQ----------------VDLGVLEDIAEAIDEE--------------EEEEEEKKKSKSNKRVPKT------VLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL-----------------TQANISDEEVLSNLEKTNRHLRLAELLREHSSDS 950
S N ++Y D+ +S +LT E P HYR L SI + RPT+ EL H + + + VE + KGKVIKFGW++GV G +I + N+VT +T++SMSAV TNG I+GGGIYYMISRSLG EFGGAIG+MFT+ANS+AV+ YI+GF +SL D+L F +D ND+R +G VTL + ILA+VGM+WVTR + LL +LI SQ+DFVIG+FL + + + GFVGYN ETF NL Y DY+N + FF V +VFFPAVTGIVAGANLSGDL+DP+ AIP GTL A+V+T+ SY+ Y ++G C L ASG EYL G + F NCT R C YG ND Q + S G L+Y GCFAATLSSAIASLVGAPRVLQALAKD LYP +G F KG GANNDP+RGY++VF I+F CI+I L+V++ LLSNFF+A YALINFSVFHASI+KSPGWRP+FKYYN WVSL T+LC+AVMFLMS V A+ TFV + LYL++SYRKPE NWGSS A + ++L+ + LN V++HVKN+RP +L+L+G+P R PLVDFA L+TK LSLL+CAH+ K + Q++ E ++ WL H++KAFF IT +F G + ++GLGKL+PN+++MGYK +W DP + VIH++ D +AV ILR+Q G DYS I+ E V P S D DA+ V++ VL D + E E SK +++ KT + + G+PLP ++ I F+ K+ G IDVWWLYDDGGL+LL+P+IL+ R+QF+ C +R+FSLA+K D LD R++A LL+KFRID+ DVV+I DI KKA D + EF+T + + I++ E+L+ EKTNRH+R+ ELL ++S +S
Sbjct: 49 SPNLQHSYGCDSRYKSLGQLTREVPPRVEHYRHL-SIHHE------YRPTLEEL---------HERIIPEKRERNVEAEPQEQKKGKVIKFGWLEGVFMRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRS----FG--TTIIDGASNDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNL----YPDYRNYEGVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTEYLQVKGEPNEWT--IFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRTCDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFV--PISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDPVASDSLLESSTTVTVTSKKSRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPKGLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMES 1023 HSP 2 Score: 145.976 bits (367), Expect = 1.078e-31 Identity = 71/149 (47.65%), Postives = 101/149 (67.79%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL-----------------TQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
K+ G IDVWWLYDDGGL+LL+P+IL+ R+QF+ C +R+FSLA+K D LD R++A LL+KFRID+ DVV+I DI KKA D + EF+T + + I++ E+L+ EKTNRH+R+ ELL ++S +S +V+A
Sbjct: 881 KQPKGLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMA 1029
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1036124418|gb|ANJ60741.1| (Na+/K+/2Cl- cotransporter [Scylla paramamosain]) HSP 1 Score: 859.751 bits (2220), Expect = 0.000e+0 Identity = 464/984 (47.15%), Postives = 616/984 (62.60%), Query Frame = 0
Query: 49 LTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGV---------------------------LGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK--YGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTM--------RECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEH-----------LVKIPF-----------------SDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQANISDE-------------EVLSNLEKTNRHLRLAELLREHSSDSETV 953
LT EALP E HYR L SI+ + RPT+ +L E + ++ E+ + P+GKVIKFGW++GV L LL + + N+VT LTTLSMSAV+TNG I+ GG+YYMISRSLG EFGG+IG+MFT+ANSIA ATYIIGF DSL D++ FDG A VD +ND R +G +TL CVL LA+VGM+WVTRV+ LL LLI SQ+DFV+G F+ +++ + GF+G ++E KN+ +Y +++N FF V VFF AVTGIVAGANLSGDL+DPA AIPKGTL A++ T+ +YIIY +IG+ L +A+G + Y+ Y N++ F NC++ + C +G N Q + +S G L+YAGCFAATLSSAIASLVGAPRVLQALAKD LYP + +F KG GANNDP+RGYV+VF I+F CIMIG+L+VVS+LLSNFF+A Y+LINFS FHAS+ KSPGWRPSFKYYN W+S ILC+ VMFL+ VTA+ITF+ LYLF+SYR P NWGSS AQ +V++L+ LN++++HVKNYRP IL+LSG R PL+DFA +TK +SLL C HV + Q++ ++ +WL H I+AF+S+ N +GA + L GLGKL+PN V++GYK++W + DP ++ +H + D+ V ILR+ G DYS I+ E + + P S+A SP +E A D E ++++ S +N + G G L ++ I FK K++ G IDVWWLYDDGGL+LL+P+ILT R+Q++ C +RVF+LA + D LDM+ RSMANLLAKFRID+ DV++IPD+AKKA++ + EF ++ DE E L EKTNRH+RL ELL E+S DS V
Sbjct: 53 LTREALPKESHYRNLDSIVD-----GSARPTLDDLHHNTLRENQRQQGIDAEA-------AAANPRGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQSGIILALLTVILGNVVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLQDLM---FFYFDG-AKIVDGEVNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQNDLQRAQGFIGLSSEVMAKNV-GPDYREFENRGQ--NFFSVFGVFFTAVTGIVAGANLSGDLKDPAEAIPKGTLAAILTTFCTYIIYPIMIGAAVLRDATGDKDVYVMY-QNLSIDENPAFTNCSLIGKVDNGTQVCPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYVLVFVISFVCIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWVGGILCLIVMFLIDWVTALITFLITVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNSIEEHVKNYRPQILVLSGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLTRHSIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPLELKAYFNTLHEALDMYFGVVILRVPQGLDYSQIIEDEDSPITMNGNESAITQSPEDKPGQSTANQLTQDGTDSEASSPPGSPQ---VERSAATTDANGENSKKRRTSMAN------LFRGPGGTELSKDVLNNITMFKRKQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFRTKDEVDKENDGLMISEAEFLGQREKTNRHIRLRELLLENSRDSSLV 1007 HSP 2 Score: 160.614 bits (405), Expect = 2.949e-36 Identity = 77/144 (53.47%), Postives = 101/144 (70.14%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQANISDE-------------EVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
K++ G IDVWWLYDDGGL+LL+P+ILT R+Q++ C +RVF+LA + D LDM+ RSMANLLAKFRID+ DV++IPD+AKKA++ + EF ++ DE E L EKTNRH+RL ELL E+S DS VV+
Sbjct: 866 KQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFRTKDEVDKENDGLMISEAEFLGQREKTNRHIRLRELLLENSRDSSLVVM 1009
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1045308339|gb|ANU78813.1| (Na+/K+/2Cl- cotransporter [Portunus trituberculatus]) HSP 1 Score: 858.981 bits (2218), Expect = 0.000e+0 Identity = 473/1026 (46.10%), Postives = 630/1026 (61.40%), Query Frame = 0
Query: 6 LDNVAFQKSEKGEPPNGGFYQSTSISENNMNTYVYDADQSFRK-LTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGV---------------------------LGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK--YGFVGYNTETFTKNLLTTNYHDYKN-PKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTM--------RECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSE--------HLVKIPFSDADSPDAQVDLGVLEDIAE-----------------AIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQANISDE-------------EVLSNLEKTNRHLRLAELLREHSSDSETV 953
LD+V+ + G +T+ S +N + Y +S R LT EALP E HYR L SI+ + S RPT+ +L E + ++ E+ + P+GKVIKFGW++GV L +L + + N+VT LTTLSMSAV+TNG I+ GG+YYMISRSLG EFGG+IG+MFT+ANSIA ATYIIGF DSL D++ FDG A VD +ND R +G +TL CVL LA+VGM+WVTRV+ LL LLI SQ+DFV+G F+ +++ + GF+G + E KN+ DY+N P FF V VFF AVTGIVAGANLSGDL+DPA AIPKGTL A+V T+ +YIIY +IG+ L +A+G Y Y N + F NC+ + C +G N Q + +S G L+YAGCFAATLSSAIASLVGAPRVLQALAKD LYP + +F KG GANNDP+RGY++VF I+F CIMIG+L+VVS+LLSNFF+A Y+LINFS FHAS+ KSPGWRPSFKYYN W+S ILC+ VMFL+ +TA+ TF+ I LYLF+SYR P NWGSS AQ +V++L+ LNT+++HVKNYRP IL+L+G R PL+DFA +TK +SLL C HV + Q++ ++ +WL HRI+AF+S+ N +GA + L GLGKL+PN V++GYK++W + DP ++ +H + D+ + ILR+ G DYS I+ E + I + D P + +D + A D E ++++ S +N + G G L ++ I FK K++ G IDVWWLYDDGGL+LL+P+ILT R+Q++ C +RVF+LA + D LDM+ RSMANLLAKFRID+ DV++IPD+AKKA++ + EF ++ DE E+L EKTNRH+RL ELL E+S DS V
Sbjct: 11 LDSVSHVQVASGLDGMDSHLHATTHSHSN-SVYETRYQKSLRHYLTREALPKESHYRNLDSIV----DGSGPRPTLDDLHHNTLRENQRQQGIDAEA-------AAANPRGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQSGIVLAILTVILGNVVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLKDLMFYY---FDG-AKIVDGAVNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQNDVQRSQGFIGLSGEVLAKNV----GPDYRNFEGRPQNFFSVFGVFFTAVTGIVAGANLSGDLKDPAEAIPKGTLAAIVTTFCTYIIYPIMIGAAVLRDATGDVNVYQMY-QNHSIDENPAFTNCSTTGHVDNGTQVCPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYILVFVISFICIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWLGGILCLIVMFLIDWITALATFLIIVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNTIEEHVKNYRPQILVLTGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLIRHRIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPMELKAYFNTLHEALDMYFGLVILRVPQGLDYSQIIEDEDSPITMNGNEGAITQTTGDKPGQSTANQLTQDGTDSEASSPPGSPQVERSTVATDANTENSKKRRTSLAN------LFRGPGGSELSKDVLNNITMFKRKQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKTKDEVDKENDGLLISEAELLGQREKTNRHIRLRELLLENSRDSSLV 1009 HSP 2 Score: 162.155 bits (409), Expect = 1.095e-36 Identity = 91/198 (45.96%), Postives = 120/198 (60.61%), Query Frame = 0
Query: 911 EFKTILTQANISDEEVLSNLEKTNRHLRLAELLR-----EHSSDS-ETVT----KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQANISDE-------------EVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
E T+ T AN + + R LA L R E S D +T K++ G IDVWWLYDDGGL+LL+P+ILT R+Q++ C +RVF+LA + D LDM+ RSMANLLAKFRID+ DV++IPD+AKKA++ + EF ++ DE E+L EKTNRH+RL ELL E+S DS VV+
Sbjct: 822 ERSTVATDANTENSK--------KRRTSLANLFRGPGGSELSKDVLNNITMFKRKQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKTKDEVDKENDGLLISEAELLGQREKTNRHIRLRELLLENSRDSSLVVM 1011
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1325273257|ref|XP_023321269.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Eurytemora affinis]) HSP 1 Score: 852.047 bits (2200), Expect = 0.000e+0 Identity = 470/999 (47.05%), Postives = 637/999 (63.76%), Query Frame = 0
Query: 7 DNVAFQKSEKGEPPNGGFYQSTSISENNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTF-----LPAKSEAKY--GFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFD--NCT-----MRE--------CQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQA---NISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
+N+ F+ +PP G + + + +TYVY+ ++S R+LT+EALPSE +YR+L+ I D +RPTI EL + + + EV+ GKV+KFGWI+GV GLL++++CN VT +TTLSMSAV+TNG I GG+YYMISR+LG EFGGAIG+MFT+ANSI+VATY +GF D+LLD++ + V F GI ++RLND+R IG + +L+LA+VGM+WVTRV+KLLL+LL+++Q+D ++GTF L KS+ ++ GF G++ ET KN + K F V VFF AVTGIVAGANLSGDL+DP+ +IPKGTL A+++TY SY +A L+G +L +ASG EY T+ +G++ + NC+ +RE C+YGS++DQ+T++ IS TGYLVY GC+ ATLSSAIASLVGAPRVLQA+ +D +YP L F G GANNDP RGY++VF IAF C+MIG L+ + SL SNFF+A YAL+N SVFH+S+TKSPGWRP+F++YNKW+SLF I+CV +MFL+ A T ++ILY + Y KPEANWGSS +A F+ +L N ALN Q H+K YRP +LLL+G P HR PL+DFANLLTKK+SLLICA++ E ++ M +E WL DH I++F+SI + G +SI L+GLGKL PN+V+MG+ S+ ++ + + + +F+ MAV ILRL NG DYS+ I +E EE E K + V G DGK LP ++V +I F+TKKR GFIDVWWLYDDGGL+LLLP+IL R QF DC +RVF L+ K + D ++RS+ANLLAKFRI F +V++IPDI KKA K EF+ I+++ ++ E+ +N E+TNRHLR AELLRE S++SE +
Sbjct: 7 ENIGFEGDNVQQPP--GAFLAVGQQASGGHTYVYEPNKSLRRLTIEALPSEENYRKLSQIYMD------QRPTIEELMNDEKIQRIDEEKAEVKPAL----------TGKVVKFGWIEGVFMRCLLNIWGVMLFLRLSWVVGQAGLIQGLLILTLCNAVTAITTLSMSAVATNGQIASGGVYYMISRALGPEFGGAIGLMFTLANSISVATYTLGFCDNLLDLIYD-VSDFGGIVADPENRLNDLRLIGTPVIVLILLLAIVGMDWVTRVQKLLLVLLVLAQIDMIVGTFFTGDSLYMKSDERHAKGFTGWSLETGEKNYFEQYLVNESGTKE--SFMSVFGVFFTAVTGIVAGANLSGDLKDPSTSIPKGTLYAIIVTYISYAFFAVLVGFNFLPQASGNVIEY-------TNNTGMYPEIFNCSEEANRIREEFNLSATSCKYGSAHDQKTMTLISATGYLVYLGCYGATLSSAIASLVGAPRVLQAVGRDKIYPKLEFFAVGHGANNDPFRGYILVFVIAFGCMMIGELNTIGSLASNFFLAAYALMNLSVFHSSMTKSPGWRPTFRFYNKWLSLFGAIVCVVLMFLLDWRLACGTTALVAILYGIMYYLKPEANWGSSAEALVFLNALNNTYALNEQQDHIKTYRPKVLLLTGNPIHRQPLLDFANLLTKKVSLLICANIVNEDEKDQIT-SMKESVELWLKDHNIRSFYSIIPNNKLSLGVQNSILLSGLGKLTPNMVIMGFNSN-NQNIHSIQEYFQTLVFAFENKMAVGILRLPNGNDYSSYIATEETTI------------------------------EEVEANKKGKKDKKVTAVFRGKDGKVLPKQIVDDITQFRTKKREGFIDVWWLYDDGGLTLLLPYILQTRKQFRDCKLRVFLLSNKAEDFDSETRSLANLLAKFRISFSEVLVIPDITKKAKPETKAEFEEIVSKYPKDSVDMSELNANAERTNRHLRTAELLREKSANSELI 945 HSP 2 Score: 164.466 bits (415), Expect = 1.830e-37 Identity = 78/135 (57.78%), Postives = 102/135 (75.56%), Query Frame = 0
Query: 954 TKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQA---NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
TKKR GFIDVWWLYDDGGL+LLLP+IL R QF DC +RVF L+ K + D ++RS+ANLLAKFRI F +V++IPDI KKA K EF+ I+++ ++ E+ +N E+TNRHLR AELLRE S++SE +V+
Sbjct: 813 TKKREGFIDVWWLYDDGGLTLLLPYILQTRKQFRDCKLRVFLLSNKAEDFDSETRSLANLLAKFRISFSEVLVIPDITKKAKPETKAEFEEIVSKYPKDSVDMSELNANAERTNRHLRTAELLREKSANSELIVL 947
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|6179898|gb|AAF05702.1|AF190129_1 (Na+/K+/2Cl- cotransporter [Callinectes sapidus]) HSP 1 Score: 851.662 bits (2199), Expect = 0.000e+0 Identity = 460/983 (46.80%), Postives = 614/983 (62.46%), Query Frame = 0
Query: 52 EALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKT--PKGKVIKFGWIDGV---------------------------LGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK--YGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTM--------RECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSE---------------------------HLVKIPF-SDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQANISDE-------------EVLSNLEKTNRHLRLAELLREHSSDSETV 953
ALP E HYR L SI+ + S RPT+ +L H + E + + ++D T P GKVIKFGW++GV L L+ + + N+VT LTTLSMSAV+TNG I+ GG+YYMISRSLG EFGG+IG+MFT+ANSIA ATYIIGF DSL D++ FDG A VD +ND R +G +TL CVL LA+VGM+WVTRV+ LL LLI SQ+DFV+G F+ + + + GF+G++ E KN+ +Y D++N FF V VFF AVTGIVAGANLSGDL+DPA AIPKGTL A++ T+ +YIIY +IG+ L +A+G + YL Y N + F NC++ + C++G N Q + +S G L+YAGCFAATLSSAIASLVGAPRVLQALAKD LYP + +F KG GANNDP+RGY++VF I+F CIMIG+L+VVS+LLSNFF+A Y+LINFS FHAS+ KSPGWRPSFKYYN W+S ILC+ VMFL+ +TA+ TF+ I LYLF+SYR P NWGSS AQ +V++L+ LNT+++HVKNYRP IL+L+G R PL+DF+ +TK +SLL C HV + Q++ ++ +WL H I+AF+S+ +GA + L GLGKL+PN V++GYK++W + +P ++ +H + D+ V ILR+ G DYS I+ E L + S+A SP +E A +D E ++++ S +N + G G L ++ I FK K++ G IDVWWLYDDGGL+LL+P+ILT R+Q++ C +RVF+LA + D LDM+ RSMANLLAKFRID+ DV++IPD+AKKA++ + EF ++ DE E+L EKTNRH+RL ELL E+S DS V
Sbjct: 32 RALPKESHYRNLDSIV----DGSGPRPTLDDL--------HHNTLRENQRQQGIDDAEAATANPSGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQAGIILALMTVILGNIVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLKDLMFYY---FDG-AKIVDGAVNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQDDLQRSQGFIGFSGEVMAKNV-GPDYRDFENRGQ--NFFSVFGVFFTAVTGIVAGANLSGDLKDPADAIPKGTLAAILTTFCTYIIYPIMIGAAVLRDATGDKDVYLMY-QNHSIDENPAFTNCSLTGSVDNGTQVCKFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYILVFVISFVCIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWLGGILCLIVMFLIDWITALATFLIIIALYLFVSYRNPNVNWGSSTQAQTYVSALKTALDLNTIEEHVKNYRPQILVLTGPVGSRPPLIDFSYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLTRHSIRAFYSLVEGSTLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCEPKELKAYFNTLHEALDMYFGVVILRVPQGLDYSQIIEDEDSPITMNGNEGAITQTTDDKPGQSSANQLTQDGTDSEASSPPGSPQ---VERSAAVVDANGENSKKRRTSLAN------LFRGPGGSELSKDVLNNITMFKRKQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKTKDEVDKESDGLLISEAELLGQREKTNRHIRLRELLLENSRDSTLV 985 HSP 2 Score: 160.999 bits (406), Expect = 1.914e-36 Identity = 77/144 (53.47%), Postives = 102/144 (70.83%), Query Frame = 0
Query: 955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQANISDE-------------EVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
K++ G IDVWWLYDDGGL+LL+P+ILT R+Q++ C +RVF+LA + D LDM+ RSMANLLAKFRID+ DV++IPD+AKKA++ + EF ++ DE E+L EKTNRH+RL ELL E+S DS VV+
Sbjct: 844 KQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKTKDEVDKESDGLLISEAELLGQREKTNRHIRLRELLLENSRDSTLVVM 987 The following BLAST results are available for this feature:
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 5
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold609_size125094:96554..118609- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold609_size125094-processed-gene-0.16 ID=snap_masked-scaffold609_size125094-processed-gene-0.16|Name=af190129_1na+ k+ 2cl- cotransporter|organism=Tigriopus kingsejongensis|type=gene|length=22056bp|location=Sequence derived from alignment at scaffold609_size125094:96554..118609- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'af190129_1na+ k+ 2cl- cotransporter' has the following synonyms
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