BLAST of alanyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000005203 (supercontig:LSalAtl2s:LSalAtl2s274:26570:29820:-1 gene:EMLSAG00000005203 transcript:EMLSAT00000005203 description:"maker-LSalAtl2s274-augustus-gene-0.21")

HSP 1 Score: 526.939 bits (1356), Expect = 5.590e-174
Identity = 321/806 (39.83%), Postives = 470/806 (58.31%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQ-GHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGF-----DKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVF--------EEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGS--PDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLI------SAAAKVTNSR------DLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQ-LGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESC------MSQ-----------------QRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTA---TAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKE-KGAQALFGEKYEDQVRVVAM----------------DDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEK 734
            +K  +IR  F+++F+ +  H    SSS IP +DP+LLF NAGM QFK VF+G      DK    RAV SQ+CVRAGGKHNDLE+VG  + HHTFFEMLGN+SFGDYFK+E  E++W  LTKEL +P+D+L+++ F        + D + + IW+  +GVP+ER++    KDNFW MGDTGPCGPCSEI +D      GG   +   ++D    +EIWNLVF+Q+NR+AD  +  LPK  +D G+GLER+ + +Q+  SNY+ D+F  +       S A+K    +      D  + +  V+ADHIR+   +I DG  P N GRGYVLRRI+RR IR   + L  +  F   L+D ++S +G  +PEL +    ++ ++  E  +FL+TL+ G  LLD+ I  +  K +PGDV ++LYDTYGFP+DLT  +  ER +T+DL+G+E        MSQ                 Q  Q +S ++   K + H +   +   + Y  +   A I+AL+ + + V ++  G +  ++LD T FYAE GGQ  D+G I      + EFKV + Q  A  V+ H+G + +GE+KI ++V  Q++ I R+    NH+ TH+L+ ALR VL     QKGSLV S++LRFDF+  + +T+DQ+  V   VN+ IR N E+   +  L  AK  +G +A+F E Y D VRVV++                   S+E CGGTH+ R G++ +F I+SE  IA G+RRI A+T   A         K    + +L+  VD +   +++    ++E+ K
Sbjct:    5 LKADEIRKLFLDFFSKKKDHTYYHSSSTIPLDDPTLLFINAGMNQFKSVFVGTIDPNNDKAKLVRAVNSQKCVRAGGKHNDLEDVGKDSYHHTFFEMLGNWSFGDYFKKEICEWSWEFLTKELKMPEDRLYVSYFGGNEKTGLDPDLDCKQIWL-DLGVPEERLLPGNMKDNFWEMGDTGPCGPCSEIHFDR----VGGRNAAHLVNQDDPMVLEIWNLVFIQFNREADKSLKLLPKKHIDCGLGLERLVSAIQNKSSNYDTDIFTPIFEAIHERSGASKPYQGKFGDEDPDQIDMAYRVVADHIRTLTIVIADGGRPDNVGRGYVLRRILRRGIRFATEKLNAKPGFFGSLVDVVVSVLGHIFPELTKDPELIKDVINEEEIQFLKTLNRGRKLLDRTIGKMDSKILPGDVAWRLYDTYGFPIDLTQLMVEERNMTVDLEGYEKAKEKAVLMSQGKGAGTEDRLALDVHSIQELQDKSISTTDDKFKYHYEEKSSDFDSDYEFSGIEATIMALRYEKKFVDSIACGTECGIILDHTSFYAEQGGQIYDEGFIVKVNDDSVEFKVKNVQVRAGYVL-HVGTLGEGELKIGEKVFLQIDTIRRQNVMKNHTGTHILNFALRQVLEGDSDQKGSLVASDKLRFDFTCKKAMTTDQVKAVEMCVNEIIRKNEEIFAKEASLAVAKTIQGLRAVFDETYPDPVRVVSVGIRVEKMEADPTGAAGSVTSVEFCGGTHLKRAGHLQVFVISSEEAIAKGIRRIIALTGPEA--------KKATQKAVLLQNKVDKVSLVVQEAKLSSKEMVK 796          

BLAST of alanyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000005940 (supercontig:LSalAtl2s:LSalAtl2s320:546129:548925:-1 gene:EMLSAG00000005940 transcript:EMLSAT00000005940 description:"maker-LSalAtl2s320-augustus-gene-4.11")

HSP 1 Score: 255.758 bits (652), Expect = 4.260e-73
Identity = 200/684 (29.24%), Postives = 321/684 (46.93%), Query Frame = 0

Query:   91 MLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNL------ISAAAKVTNSRD-LKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIR---HGYQLGCQTPFMHLLIDSLISEMGEAYPE-----LVQQKAHLEKILYNENERFLETLDLGLGLLDK---------------------EIAVLSD---------------KQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHI-----------DGIENTL------FNGYTQTSGSAKIIAL---QVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAE--FKVCDTQKFANK-VIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYE-PITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQA----LFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAID 695
            MLG++SF DYFK++AI+FAW  LT  L +PK+ L +T F++D E  + W+   G+  +++I  G  DNFW MG TGPCGPC+EI Y                   +  EIWNLVFM+Y R  +G +  L K  VDTGMGLER+ ++L    SNY+ DLF+ +      +S A   + S D L++ +  ++ADH R  A  + DG+ P      Y LR IIRR ++     +    Q  F+  LI  +   +G+ + E      ++ +A L  +L  E++   E       LL+K                      I    D               K I G+  FKLYD+ GF  +     A       D   F    ++ ++Q++   S V++T + I           D  +         +  Y   S   ++I +   + D + V  L  G   +L+ +++PFY+E+GGQ    G I  +  E  F V D   F+ K V+ H+G V  G     D V  +++   R     NH+ +HLL+AA+  VL     Q+   V+    +F    ++  ++S +++ +    NQ I +   +   +    ++  K +++    L GEKY D+V ++ +  FS E C GTHV+ T ++   +IT    +++G++    +T Q AID
Sbjct:    1 MLGSWSFNDYFKKDAIQFAWEFLTGPLEIPKEFLNVTYFKDDLETREYWLSQ-GLSPDKIIPKGETDNFWEMGSTGPCGPCTEIHY-------------------KDTEIWNLVFMEYVRRPNGELASLDKKHVDTGMGLERLTSILNGYTSNYDTDLFIPIFDKISKLSGADPYSGSFDKLQDINYRILADHSRMIATCLSDGLLPD---VSYRLRNIIRRCMKISAESFNSKNQVDFLKELIYCVSESLGDHHREYLESPFLKDEAKLSLLLKFEHDVLKELEKTHKSLLNKLKDAVPFKTLFEKYESELDPSMIGRFYDAFKMYSCNALNNAEPKIISGEFAFKLYDSIGFKSEDIELFALLLDAKYDPYEFHLYSAKVKEQSKVTTS-VNRTNMEILSKITLAPKTDDSFKYKWVQSGFKYQFYIIESNILQLIQISNDKSDSQCVGKLNRGDTGILICEKSPFYSEAGGQAXXTGSITGSLGESVFHVHDV--FSLKGVVYHVGTVHSGTFMRQDLVKLKIDPFKRVTHMQNHTGSHLLNAAMNTVLP-FTTQRSLRVDXTGFKFXACIFKLNLSSSEINDIEEKANQMINNADPIVQAKDKYLDSFIKNSKSKVLTLPGEKYPDKVSMICLPQFS-EPCCGTHVNCTKDVEALTITHIKSVSSGIKSFRCLTGQKAID 656          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830024|sp|A8ANQ1.1|SYA_CITK8 (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 959.133 bits (2478), Expect = 0.000e+0
Identity = 476/836 (56.94%), Postives = 602/836 (72.01%), Query Frame = 0

Query:    4 SQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKE--LGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVS--KTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQ-----------SKNTVAIYVEKYGGTSVANVE--RINAVADNVERSHKLGHKLVVVLSAMSGETNRLLGLAKEIHPDAGAREIDVLVS 815
            ++IR  F+++F+S+GH+ VASSSL+P NDP+LLFTNAGM QFKDVFLG DKR+Y RA TSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI+FAW LLT E    LPKD+LW+TV+E DDEA +IW K +G+P+ER+IRIG       A DNFW MGDTGPCGPC+EIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ+NR ADG M PLPKP VDTGMGLERIAAVLQHV+SNYEIDLF  LI A AKVT + DL NKSL VIADHIRS AFLI DGV PSNE RGYVLRRIIRRA+RHG  LG +  F + L+  LI  MG A  EL +Q+A +E++L  E E+F  TL+ GL LLD+E+A LS   + G+  F+LYDTYGFPVDLT DV RER + +D  GFE+ M +QR++AR A+ F +     + +DG     F GY     + K+ AL VD +AV  ++ G++AV+VLDQTPFYAESGGQ GDKG +K A   F V DTQK+  + IGHIG +  G +K+ D V   V+D  R + + NHSATHL+HAALR +LG HV QKGSLV  + LRFDFSH E +   ++  V  +VN QIR N  V T  MDLE AK KGA ALFGEKY+D+VRV++M DFS ELCGGTH SRTG+IGLF I SE+G AAGVRRIEA+T + AI     +  +L+ ++Q+LK +  ++  K+  +L++ R+LEK+ QQL+           S   V I   K   + +A VE   +  + D+++  ++LG  ++V+ +   G+ + + G++K++     A E+  +V+
Sbjct:    6 AEIRQAFLDFFHSKGHQVVASSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTGENWFALPKDRLWVTVYETDDEAYEIWEKEVGIPRERIIRIGDNKGAAYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYEIDLFRTLIEAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVVPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEELKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDGASE--FKGYDHLELNGKVTALFVDGKAVDAISAGQEAVVVLDQTPFYAESGGQVGDKGELKGAGFAFAVSDTQKYG-QAIGHIGTLSTGSLKVGDAVQADVDDARRARIRLNHSATHLMHAALRQILGTHVAQKGSLVNDKVLRFDFSHNEAMKPSEIRAVEDLVNAQIRRNLPVETNIMDLEAAKAKGAMALFGEKYDDRVRVLSMGDFSTELCGGTHASRTGDIGLFRIVSESGTAAGVRRIEAVTGEGAIATLHAESDRLSDIAQLLKGDSQNLGEKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAVDINGVKLLVSELAGVEPKMLRTMVDDLK--NQLGSTVIVLATVAEGKVSLIAGVSKDVTDRVKAGELVGMVA 836          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|122257933|sp|Q02I63.1|SYA_PSEAB (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 956.051 bits (2470), Expect = 0.000e+0
Identity = 461/758 (60.82%), Postives = 575/758 (75.86%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKE--LGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVS--KTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAM-DDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSKNTVA 746
            MK ++IR  F+ +F  +GH  VASSSLIP NDP+LLFTNAGM QFKD FLG +KR Y RA TSQ+CVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK++AI +AW  LT E  L LPK+KLW+TV+  DDEA DIW K +GVP ER++RIG       A DNFWAMGDTGPCGPC+EIF+DHG ++ GGPPGSP+EDGDRYIEIWN VFMQ+NR ADGVM PLP P VDTGMGLER++AVLQHVHSNYEIDLF NL+ A+A+     +    SL V+ADHIRS +FLI DGV PSNEGRGYVLRRIIRRA RHG +LG +  F H ++ +L++EMG+A+PEL QQ+AH+E++L  E E+F +TL+ GL +L++++A L    IPG+V+FKLYDTYGFPVDLTND+ARER LT+D DGFE  M  QR++AR++++F     + V +DG   T F GY   SG+ +I+AL  D +AV+ L  G++ V+VLDQTPFYAESGGQ GD G ++ A   F V DT K     + H G V +G + +   V  +V+   R+ T  NHSATHLLHAALR VLGDHVQQKGSLV+S+RLRFDFSH+E I ++QL  +  IVN +IR N EV T + D++ AK KGA ALFGEKY DQVRV++M  DFS+ELCGGTHVSRTG+IGLF ITSE G+AAGVRRIEA+T  AA+ + +    +L   + ++K + D++  K+  LL++NR LEK+ +QL++K   A
Sbjct:    1 MKSAEIREAFLRFFEEKGHTRVASSSLIPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWEFLTGEKWLNLPKEKLWVTVYATDDEAYDIWTKEVGVPAERMVRIGDNKGAPYASDNFWAMGDTGPCGPCTEIFFDHGPEIWGGPPGSPEEDGDRYIEIWNNVFMQFNRTADGVMHPLPAPSVDTGMGLERVSAVLQHVHSNYEIDLFQNLLKASAEAIGCANDDAPSLKVVADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRACRHGNKLGARGTFFHKIVAALVAEMGDAFPELKQQQAHIERVLKTEEEQFAKTLEQGLKILEQDLAELQGSVIPGNVVFKLYDTYGFPVDLTNDIARERELTIDEDGFEREMEAQRERARASSAFGMDYNSLVKVDG--ETRFLGYQGVSGAGQIVALFRDGQAVERLEEGEEGVVVLDQTPFYAESGGQVGDSGYLEAAGVRFDVRDTTKAGGAHLHH-GVVARGNLSVGAAVKAEVDASVRQATALNHSATHLLHAALRQVLGDHVQQKGSLVDSQRLRFDFSHFEAIKAEQLKALEDIVNAEIRRNTEVETEETDIDTAKAKGAMALFGEKYGDQVRVLSMGGDFSVELCGGTHVSRTGDIGLFKITSEGGVAAGVRRIEAVTGAAALAYLNGAEEQLKEAASLVKGSRDNLLDKLGALLERNRSLEKELEQLKAKAASA 755          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|20532264|sp|Q9I553.1|SYA_PSEAE (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 954.51 bits (2466), Expect = 0.000e+0
Identity = 461/758 (60.82%), Postives = 575/758 (75.86%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKE--LGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVS--KTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAM-DDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSKNTVA 746
            MK ++IR  F+ +F  +GH  VASSSLIP NDP+LLFTNAGM QFKD FLG +KR Y RA TSQ+CVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK++AI +AW  LT E  L LPK+KLW+TV+  DDEA DIW K +GVP ER++RIG       A DNFWAMGDTGPCGPC+EIF+DHG ++ GGPPGSP+EDGDRYIEIWN VFMQ+NR ADGVM PLP P VDTGMGLER++AVLQHVHSNYEIDLF NL+ A+A+     +    SL V+ADHIRS +FLI DGV PSNEGRGYVLRRIIRRA RHG +LG +  F H ++ +L++EMG+A+PEL QQ+AH+E++L  E E+F +TL+ GL +L++++A L    IPG+V+FKLYDTYGFPVDLTND+ARER LT+D DGFE  M  QR++AR++++F     + V +DG   T F GY   SG+ +I+AL  D +AV+ L  G++ V+VLDQTPFYAESGGQ GD G ++ A   F V DT K     + H G V +G + +   V  +V+   R+ T  NHSATHLLHAALR VLGDHVQQKGSLV+S+RLRFDFSH+E I ++QL  +  IVN +IR N EV T + D++ AK KGA ALFGEKY DQVRV++M  DFS+ELCGGTHVSRTG+IGLF ITSE G+AAGVRRIEA+T  AA+ + +    +L   + ++K + D++  K+  LL++NR LEK+ +QL++K   A
Sbjct:    1 MKSAEIREAFLRFFEEKGHTRVASSSLIPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWEFLTGEKWLNLPKEKLWVTVYATDDEAYDIWTKEVGVPAERMVRIGDNKGAPYASDNFWAMGDTGPCGPCTEIFFDHGPEIWGGPPGSPEEDGDRYIEIWNNVFMQFNRTADGVMHPLPAPSVDTGMGLERVSAVLQHVHSNYEIDLFQNLLKASAEAIGCANDDAPSLKVVADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRACRHGNKLGARGTFFHKIVAALVAEMGDAFPELKQQQAHIERVLKTEEEQFAKTLEQGLKILEQDLAELQGSVIPGNVVFKLYDTYGFPVDLTNDIARERELTIDEDGFEREMEAQRERARASSAFGMDYNSLVKVDG--ETRFLGYQGVSGAGQIVALFRDGQAVERLEEGEEGVVVLDQTPFYAESGGQVGDSGYLEAAGVRFDVRDTTKAGGAHLHH-GVVARGNLSVGGAVKAEVDASVRQATALNHSATHLLHAALRQVLGDHVQQKGSLVDSQRLRFDFSHFEAIKAEQLKALEDIVNAEIRRNTEVETEETDIDTAKAKGAMALFGEKYGDQVRVLSMGGDFSVELCGGTHVSRTGDIGLFKITSEGGVAAGVRRIEAVTGAAALAYLNGAEEQLKEAASLVKGSRDNLLDKLGALLERNRSLEKELEQLKAKAASA 755          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830183|sp|A6VA71.1|SYA_PSEA7 (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 953.74 bits (2464), Expect = 0.000e+0
Identity = 462/758 (60.95%), Postives = 574/758 (75.73%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKE--LGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVS--KTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAM-DDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSKNTVA 746
            MK ++IR  F+ +F  +GH  VASSSLIP NDP+LLFTNAGM QFKD FLG +KR Y RA TSQ+CVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK++AI +AW  LT E  L LPK+KLW+TV+  DDEA DIW K +GVP ER++RIG       A DNFWAMGDTGPCGPC+EIF+DHG D+ GGPPGSP+EDGDRYIEIWN VFMQ+NR ADGVM PLP P VDTGMGLER++AVLQHVHSNYEIDLF NL+ A+A+     +    SL V+ADHIRS +FLI DGV PSNEGRGYVLRRIIRRA RHG +LG +  F H ++ +L++EMG+A+PEL QQ+AH+E++L  E E+F +TL+ GL +L++++A L    IPG+V+FKLYDTYGFPVDLTND+ARER LT+D DGFE  M  QR++AR++++F     + V +DG   T F GY   SG+ +I+AL  D +AV+ L  G++ V+VLDQTPFYAESGGQ GD G ++ A   F V DT K     + H G V +G + +   V  +V+   R+ T  NHSATHLLHAALR VLGDHVQQKGSLV+S+RLRFDFSH+E I  +QL  +  IVN +IR N EV T + D++ AK KGA ALFGEKY DQVRV++M  DFS+ELCGGTHVSRTG+IGLF ITSE G+AAGVRRIEA+T  AA+ + +    +L   + ++K + D++  K+  LL++NR LEK+ +QL++K   A
Sbjct:    1 MKSAEIREAFLRFFEEKGHTRVASSSLIPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWEFLTGEKWLNLPKEKLWVTVYATDDEAYDIWTKEVGVPAERMVRIGDNKGAPYASDNFWAMGDTGPCGPCTEIFFDHGPDIWGGPPGSPEEDGDRYIEIWNNVFMQFNRTADGVMHPLPAPSVDTGMGLERVSAVLQHVHSNYEIDLFQNLLKASAEAIGCANDDAPSLKVVADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRACRHGNKLGARGTFFHKIVAALVAEMGDAFPELKQQQAHIERVLKTEEEQFAKTLEQGLKILEQDLAELQGSVIPGNVVFKLYDTYGFPVDLTNDIARERELTIDEDGFEREMEAQRERARASSAFGMDYNSLVKVDG--ETRFLGYQGVSGAGQIVALFRDGQAVERLEEGEEGVVVLDQTPFYAESGGQVGDSGYLEAAGVRFDVRDTTKAGGAHLHH-GVVAEGGLSVGAAVKAEVDASVRQATALNHSATHLLHAALRQVLGDHVQQKGSLVDSQRLRFDFSHFEAIKPEQLKALEDIVNAEIRRNTEVETEETDIDTAKAKGAMALFGEKYGDQVRVLSMGGDFSVELCGGTHVSRTGDIGLFKITSEGGVAAGVRRIEAVTGAAALAYLNGAEEQLKEAAGLVKGSRDNLLDKLGALLERNRSLEKELEQLKAKAASA 755          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830256|sp|A4XWE2.2|SYA_PSEMY (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 950.273 bits (2455), Expect = 0.000e+0
Identity = 464/756 (61.38%), Postives = 572/756 (75.66%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLT--KELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAM-DDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSKNTVA 746
            MK ++IR  F+ +F  +GH  VASSSLIP NDP+LLFTNAGM QFKD FLG +KR Y RA TSQ+CVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK++AI +AW  LT  K L LPK+KLW+TV+  DDEA +IW K IGVP ER+IRIG       A DNFWAMGDTGPCGPC+EIF+DHGE + GGPPGSP+EDGDRYIEIWN VFMQ+NR ADGV+ PLP P VDTGMGLERI+AVLQHV+SNYEIDLF NL++A+AK     +    SL V+ADHIRS +FLI DGVTPSNEGRGYVLRRI+RRA RHG +LG +  F + ++ +L++EMGEA+PEL QQ+AH+E++L  E E+F +TL+ GL +L++++A L    IPGD++FKLYDTYGFPVDLT D+ARER L++D +GFE  M  QR++ARSA++F       +    +T F GY  TSGS KIIAL     AV +L  G++ V+VLDQTPFYAESGGQ GD G +++A   F V DT K     + H G V KG + +   V  +     R+ T  NHSATHLLHAALR VLGDHVQQKGSLV+S+RLRFDFSH+E I  +QL Q+  IVN +IR N EV T + D+E AK KGA ALFGEKY DQVRV++M  DFS+ELCGGTHVSRTG+IGLF ITSE G+AAGVRRIEA+T  AA+ W +    +L   + ++K + D++  K+  L+++NR+LEK+ +QL++K   A
Sbjct:    1 MKSAEIREAFLSFFEEKGHTRVASSSLIPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWEFLTSDKWLNLPKEKLWVTVYATDDEAYEIWNKEIGVPAERMIRIGDNKGAPYASDNFWAMGDTGPCGPCTEIFFDHGEHIWGGPPGSPEEDGDRYIEIWNNVFMQFNRTADGVLHPLPAPSVDTGMGLERISAVLQHVNSNYEIDLFQNLLAASAKAIGCANEGQASLKVVADHIRSCSFLIADGVTPSNEGRGYVLRRIVRRACRHGNKLGAKGSFFYQIVAALVAEMGEAFPELKQQQAHIERVLKTEEEQFAKTLEQGLKILEQDLAGLQGSVIPGDIVFKLYDTYGFPVDLTADIARERELSVDEEGFEREMEAQRERARSASAFGMDYNALVKVDADTRFLGYEGTSGSGKIIALFKAGAAVDSLAEGEEGVVVLDQTPFYAESGGQIGDCGYLESAGMRFDVRDTTKAGGAFLHH-GVVAKGGLSVGTSVKAEAEASVRQATALNHSATHLLHAALRQVLGDHVQQKGSLVDSQRLRFDFSHFEAIKPEQLKQLEDIVNAEIRKNSEVETEETDIETAKAKGAMALFGEKYGDQVRVLSMGGDFSVELCGGTHVSRTGDIGLFKITSEGGVAAGVRRIEAVTGAAALAWLNAAEEQLKEAASLVKGSRDNVLDKLSALIERNRQLEKELEQLKAKAASA 755          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|2507425|sp|P00957.2|SYA_ECOLI (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS >gi|205830082|sp|B1XCM5.1|SYA_ECODH RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 948.732 bits (2451), Expect = 0.000e+0
Identity = 466/837 (55.68%), Postives = 600/837 (71.68%), Query Frame = 0

Query:    4 SQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKE--LGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSK----------------NTVAIYVEKYGGTSVANVERINAVADNVERSHKLGHKLVVVLSAMSGETNRLLGLAKEIHPDAGAREIDVLVS 815
            ++IR  F+++F+S+GH+ VASSSL+P NDP+LLFTNAGM QFKDVFLG DKR+Y RA TSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI+FAW LLT E    LPK++LW+TV+E DDEA +IW K +G+P+ER+IRIG       A DNFW MGDTGPCGPC+EIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ+NR ADG M PLPKP VDTGMGLERIAAVLQHV+SNY+IDLF  LI A AKVT + DL NKSL VIADHIRS AFLI DGV PSNE RGYVLRRIIRRA+RHG  LG +  F + L+  LI  MG A  +L +Q+A +E++L  E E+F  TL+ GL LLD+E+A LS   + G+  F+LYDTYGFPVDLT DV RER + +D  GFE+ M +QR++AR A+ F +     I     + F GY     + K+ AL VD +AV  +  G++AV+VLDQTPFYAESGGQ GDKG +K A   F V DTQK+  + IGHIG++  G +K+ D V   V++  R + + NHSATHL+HAALR VLG HV QKGSLV  + LRFDFSH E +  +++  V  +VN QIR N  + T  MDLE AK KGA ALFGEKY+++VRV++M DFS ELCGGTH SRTG+IGLF I SE+G AAGVRRIEA+T + AI        +L+ ++ +LK + +++  K+  +L++ R+LEK+ QQL+ +                N V + V +  G      + +  + D+++  ++LG  ++V+ + + G+ + + G++K++     A E+  +V+
Sbjct:    6 AEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYG-QAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNTQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGV---EPKMLRTMVDDLK--NQLGSTIIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVA 836          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|123558824|sp|Q31X62.1|SYA_SHIBS (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 948.732 bits (2451), Expect = 0.000e+0
Identity = 466/837 (55.68%), Postives = 599/837 (71.57%), Query Frame = 0

Query:    4 SQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKE--LGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSK----------------NTVAIYVEKYGGTSVANVERINAVADNVERSHKLGHKLVVVLSAMSGETNRLLGLAKEIHPDAGAREIDVLVS 815
            ++IR  F+++F+S+GH+ VASSSL+P NDP+LLFTNAGM QFKDVFLG DKR+Y RA TSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI+FAW LLT E    LPK++LW+TV+E DDEA +IW K +G+P+ER+IRIG       A DNFW MGDTGPCGPC+EIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ+NR ADG M PLPKP VDTGMGLERIAAVLQHV+SNY+IDLF  LI A AKVT + DL NKSL VIADHIRS AFLI DGV PSNE RGYVLRRIIRRA+RHG  LG +  F + L+  LI  MG A  +L +Q+A +E++L  E E+F  TL+ GL LLD+E+A LS   + G+  F+LYDTYGFPVDLT DV RER + +D  GFE+ M +QR++AR A+ F +     I     + F GY     + K+ AL VD +AV  +  G++AV+VLDQTPFYAESGGQ GDKG +K A   F V DTQK+  + IGHIG++  G +K+ D V   V++  R + + NHSATHL+HAALR VLG HV QKGSLV  + LRFDFSH E +  +++  V  +VN QIR N  + T  MDLE AK KGA ALFGEKY+++VRV++M DFS ELCGGTH SRTG+IGLF I SE+G AAGVRRIEA+T + AI        +L+ ++ +LK + +++  K+  +L++ R+LEK+ QQL+ +                N V + V +  G      + +  + D+++  ++LG  ++V+ +   G+ + + G++K++     A E+  +V+
Sbjct:    6 AEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKDANFSFAVEDTQKYG-QAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGV---EPKMLRTMVDDLK--NQLGSTIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVA 836          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|123616373|sp|Q3YYG6.1|SYA_SHISS (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS >gi|205830081|sp|A7ZQC5.1|SYA_ECO24 RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 948.347 bits (2450), Expect = 0.000e+0
Identity = 466/837 (55.68%), Postives = 599/837 (71.57%), Query Frame = 0

Query:    4 SQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKE--LGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSK----------------NTVAIYVEKYGGTSVANVERINAVADNVERSHKLGHKLVVVLSAMSGETNRLLGLAKEIHPDAGAREIDVLVS 815
            ++IR  F+++F+S+GH+ VASSSL+P NDP+LLFTNAGM QFKDVFLG DKR+Y RA TSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI+FAW LLT E    LPK++LW+TV+E DDEA +IW K +G+P+ER+IRIG       A DNFW MGDTGPCGPC+EIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ+NR ADG M PLPKP VDTGMGLERIAAVLQHV+SNY+IDLF  LI A AKVT + DL NKSL VIADHIRS AFLI DGV PSNE RGYVLRRIIRRA+RHG  LG +  F + L+  LI  MG A  +L +Q+A +E++L  E E+F  TL+ GL LLD+E+A LS   + G+  F+LYDTYGFPVDLT DV RER + +D  GFE+ M +QR++AR A+ F +     I     + F GY     + K+ AL VD +AV  +  G++AV+VLDQTPFYAESGGQ GDKG +K A   F V DTQK+  + IGHIG++  G +K+ D V   V++  R + + NHSATHL+HAALR VLG HV QKGSLV  + LRFDFSH E +  +++  V  +VN QIR N  + T  MDLE AK KGA ALFGEKY+++VRV++M DFS ELCGGTH SRTG+IGLF I SE+G AAGVRRIEA+T + AI        +L+ ++ +LK + +++  K+  +L++ R+LEK+ QQL+ +                N V + V +  G      + +  + D+++  ++LG  ++V+ +   G+ + + G++K++     A E+  +V+
Sbjct:    6 AEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYG-QAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGV---EPKMLRTMVDDLK--NQLGSTIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVA 836          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830083|sp|A8A3H4.1|SYA_ECOHS (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 948.347 bits (2450), Expect = 0.000e+0
Identity = 466/837 (55.68%), Postives = 599/837 (71.57%), Query Frame = 0

Query:    4 SQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKE--LGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSK----------------NTVAIYVEKYGGTSVANVERINAVADNVERSHKLGHKLVVVLSAMSGETNRLLGLAKEIHPDAGAREIDVLVS 815
            ++IR  F+++F+S+GH+ VASSSL+P NDP+LLFTNAGM QFKDVFLG DKR+Y RA TSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI+FAW LLT E    LPK++LW+TV+E DDEA +IW K +G+P+ER+IRIG       A DNFW MGDTGPCGPC+EIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ+NR ADG M PLPKP VDTGMGLERIAAVLQHV+SNY+IDLF  LI A AKVT + DL NKSL VIADHIRS AFLI DGV PSNE RGYVLRRIIRRA+RHG  LG +  F + L+  LI  MG A  +L +Q+A +E++L  E E+F  TL+ GL LLD+E+A LS   + G+  F+LYDTYGFPVDLT DV RER + +D  GFE+ M +QR++AR A+ F +     I     + F GY     + K+ AL VD +AV  +  G++AV+VLDQTPFYAESGGQ GDKG +K A   F V DTQK+  + IGHIG++  G +K+ D V   V++  R + + NHSATHL+HAALR VLG HV QKGSLV  + LRFDFSH E +  +++  V  +VN QIR N  + T  MDLE AK KGA ALFGEKY+++VRV++M DFS ELCGGTH SRTG+IGLF I SE+G AAGVRRIEA+T + AI        +L+ ++ +LK + +++  K+  +L++ R+LEK+ QQL+ +                N V + V +  G      + +  + D+++  ++LG  ++V+ +   G+ + + G++K++     A E+  +V+
Sbjct:    6 AEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYG-QAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGV---EPKMLRTMVDDLK--NQLGSTIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVA 836          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830086|sp|B1LQ15.1|SYA_ECOSM (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 948.347 bits (2450), Expect = 0.000e+0
Identity = 466/837 (55.68%), Postives = 599/837 (71.57%), Query Frame = 0

Query:    4 SQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKE--LGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSK----------------NTVAIYVEKYGGTSVANVERINAVADNVERSHKLGHKLVVVLSAMSGETNRLLGLAKEIHPDAGAREIDVLVS 815
            ++IR  F+++F+S+GH+ VASSSL+P NDP+LLFTNAGM QFKDVFLG DKR+Y RA TSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI+FAW LLT E    LPK++LW+TV+E DDEA +IW K +G+P+ER+IRIG       A DNFW MGDTGPCGPC+EIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ+NR ADG M PLPKP VDTGMGLERIAAVLQHV+SNY+IDLF  LI A AKVT + DL NKSL VIADHIRS AFLI DGV PSNE RGYVLRRIIRRA+RHG  LG +  F + L+  LI  MG A  +L +Q+A +E++L  E E+F  TL+ GL LLD+E+A LS   + G+  F+LYDTYGFPVDLT DV RER + +D  GFE+ M +QR++AR A+ F +     I     + F GY     + K+ AL VD +AV  +  G++AV+VLDQTPFYAESGGQ GDKG +K A   F V DTQK+  + IGHIG++  G +K+ D V   V++  R + + NHSATHL+HAALR VLG HV QKGSLV  + LRFDFSH E +  +++  V  +VN QIR N  + T  MDLE AK KGA ALFGEKY+++VRV++M DFS ELCGGTH SRTG+IGLF I SE+G AAGVRRIEA+T + AI        +L+ ++ +LK + +++  K+  +L++ R+LEK+ QQL+ +                N V + V +  G      + +  + D+++  ++LG  ++V+ +   G+ + + G++K++     A E+  +V+
Sbjct:    6 AEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYG-QAIGHIGKLATGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGV---EPKMLRTMVDDLK--NQLGSTIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVA 836          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1011362591|ref|WP_062275569.1| (alanine--tRNA ligase [Marichromatium gracile] >gi|1003483950|gb|KXX64375.1| alanine--tRNA ligase [Marichromatium gracile])

HSP 1 Score: 995.342 bits (2572), Expect = 0.000e+0
Identity = 471/810 (58.15%), Postives = 604/810 (74.57%), Query Frame = 0

Query:    4 SQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLS-DKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSK------NTVAIYVEKYGGTSVANVERINAVADNVERS-----HKLGHKLVVVLSAMSGETNRLLGLAKEI 801
            +++R+ F++YF  + H++V+SS L+P NDP+LLFTNAGMVQFKDVFLG ++R Y RAVT+QRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK+EAI FAW  LT ELGLP ++LW+TV+E+DDEA DIW+K +GV  ER  R GAKDNFW+MGDTGPCGPCSEIFYDHG +VAGGPPGSPDEDGDRY+EIWNLVFMQ+NRDA+G MTPLP+P VDTGMGLER+AAVLQ VHSNY+IDLF NLI+AAA+ T   DL +KSL VIADHIRS+AFLIVDGVTP NEGRGYVLRRI+RRAIRHGY+LG   PF H L+ +L++EMG AYPELV  +AH+E+I+  E ERF ETL+ G+ +L++ IA L+ ++ IPG+ +FKLYDTYGFP DLT D+ARERGL +DL+GFE+ M+ Q+ +AR+A+ F     V ID    T F GY +    A ++AL  D  +  +L  G++ +++LD TPFY ESGGQ GD+G I ++   F+V DT K    ++ H+G++ KGE+ + D V  +V+   R     NHSATHLLHAALR VLG+HVQQKGSLV  ERLRFDFSH+EP++ +QL ++  +VN QIR N  V T  M LE+AK  GA ALFGEKY DQVRV+ M +FS+ELCGGTHV   G+IGL  I SE GIA+GVRRIEA+T   A+DW +    ++  ++ +LK   + ++ ++EQLL+++R LEK+ +QL+SK        +A   E+  G  V   E   A    +  +      KLG  ++V+ +   G+   + G+ K++
Sbjct:    5 AELRASFLDYFAQRQHEQVSSSPLVPANDPTLLFTNAGMVQFKDVFLGRERRGYSRAVTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIGFAWQYLTGELGLPAERLWVTVYEDDDEAADIWLKEVGVSAERFSRCGAKDNFWSMGDTGPCGPCSEIFYDHGPEVAGGPPGSPDEDGDRYVEIWNLVFMQFNRDAEGNMTPLPRPSVDTGMGLERLAAVLQGVHSNYDIDLFRNLIAAAAEATGCEDLGDKSLRVIADHIRSSAFLIVDGVTPGNEGRGYVLRRIMRRAIRHGYRLGQSQPFFHRLVAALVAEMGAAYPELVAAQAHVERIVLLEEERFAETLEHGMRILEEAIAGLAGERIIPGETVFKLYDTYGFPTDLTADIARERGLGVDLEGFEAAMADQKARARAASQFGGGQDVEIDLQGETEFRGYERCEDEATVVALYRDGESCDSLGAGERGMVILDLTPFYGESGGQVGDRGWIASSGGRFEVTDTLKKGGALVCHLGELRKGELHVGDAVRAEVDAERRRAIALNHSATHLLHAALRQVLGEHVQQKGSLVGPERLRFDFSHFEPVSREQLREIERLVNSQIRANHLVETRIMSLEDAKASGAMALFGEKYADQVRVLRMGEFSVELCGGTHVKAVGDIGLMKIVSEAGIASGVRRIEAVTGAGALDWVEADESRVLRIAGLLKGAREDVDERVEQLLERSRRLEKELEQLKSKLASAAGQDLAAQAEEIAGVRVLAAELEGADPKGLRETVDQLKDKLGSAVIVLATVAEGKVALVAGVTKDL 814          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|491366125|ref|WP_005224036.1| (alanine--tRNA ligase [Marichromatium purpuratum] >gi|570733796|gb|AHF04959.1| alanyl-tRNA synthetase [Marichromatium purpuratum 984])

HSP 1 Score: 992.645 bits (2565), Expect = 0.000e+0
Identity = 471/810 (58.15%), Postives = 602/810 (74.32%), Query Frame = 0

Query:    4 SQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDK-QIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSK------NTVAIYVEKYGGTSVANVERINAVADNVERS-----HKLGHKLVVVLSAMSGETNRLLGLAKEI 801
            +++R+ F++YF  + H++V+SS L+P NDP+LLFTNAGMVQFKDVFLG ++R Y RAVT+QRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK+EAI FAW  LT ELGLP ++LW+TV+E+DDEA DIW+K IGV  ER  R GAKDNFW+MGDTGPCGPCSEIF+DHG ++AGGPPGSPDEDGDRY+EIWNLVFMQ+NRDA+G MTPLP+P VDTGMGLER+AAVLQ VHSNY+IDLF NLI+AAA+ T   DL +KSL VIADHIRS+AFLIVDGVTP NEGRGYVLRRI+RRAIRHGY+LG   PF H L+ +L++EMG AYPELV  +AH+E+I+  E ERF ETL+ G+ +LD+ IA L D   IPG+ +FKLYDTYGFP DLT D+ARERGL +DL+GFE+ M+ Q+ +AR+A+ F     V ID    T F GY +    A ++AL  D  +  +L  G++ +++LD TPFY ESGGQ GD+G I  +   F+V DTQK    ++ HIG++ +GE+ + D V  +V+   R     NHSATHLLHAALR VLG+HVQQKGSLV  ERLRFDFSH+EP++ +QL ++  +VN +IR N  V T  M LE+AK  GA ALFGEKY DQVRV+ M +FS+ELCGGTHV   G+IGL  I SE GIA+GVRRIEA++   A+DW +    ++  ++ +LK   + ++ ++EQLL+++R LEK+ +QL+SK        +A   E+  G  V   E   A    +  +      KLG  ++V+ +   G+   + G+ K++
Sbjct:    5 AELRASFLDYFAQRQHEQVSSSPLVPANDPTLLFTNAGMVQFKDVFLGRERRGYSRAVTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIGFAWQYLTGELGLPAERLWVTVYEDDDEAADIWLKEIGVSAERFSRCGAKDNFWSMGDTGPCGPCSEIFFDHGPEIAGGPPGSPDEDGDRYVEIWNLVFMQFNRDAEGNMTPLPRPSVDTGMGLERLAAVLQGVHSNYDIDLFRNLIAAAAEATGCEDLGDKSLRVIADHIRSSAFLIVDGVTPGNEGRGYVLRRIMRRAIRHGYRLGQSQPFFHRLVAALVAEMGAAYPELVAAQAHVERIVRLEEERFAETLEHGMRILDEAIAGLGDTCTIPGETVFKLYDTYGFPTDLTADIARERGLGVDLEGFEAAMADQKARARAASQFSGGQDVEIDLQGETEFRGYERCEDEATVVALYRDGESCDSLGGGERGMVILDLTPFYGESGGQVGDRGWITGSGGRFEVTDTQKKGGALVCHIGELREGELCVGDMVCAEVDGERRRAIALNHSATHLLHAALRRVLGEHVQQKGSLVGPERLRFDFSHFEPVSREQLREIERLVNGEIRANHLVETRIMSLEDAKASGAMALFGEKYADQVRVLRMGEFSVELCGGTHVKAVGDIGLMKIVSEAGIASGVRRIEAVSGAGALDWIEADEQRVLRIAGLLKGAREDVDERVEQLLERSRRLEKELEQLKSKLASAAGQDLAAQAEEVAGVRVLAAELEGADPKGLRETVDQLKDKLGSAVIVLATVSEGKVALVAGVTKDL 814          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|931457615|gb|KPK40731.1| (alanyl-tRNA synthetase [Gammaproteobacteria bacterium SG8_47])

HSP 1 Score: 981.474 bits (2536), Expect = 0.000e+0
Identity = 464/743 (62.45%), Postives = 581/743 (78.20%), Query Frame = 0

Query:    1 MKVS-QIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSK 742
            MK S Q+R  F++YF+  GH+ VASS L+P NDP+LLFTNAGMVQFKDVFLG ++R   RA +SQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK+EAI FAW  LT  +GL  +KLW+TVFEEDDEA DIW+K + V   R  RIGAKDNFW+MGDTGPCGPCSEIFYDHGE VAGGPPG+P+ DGDRY+EIWNLVFMQY+R  DG +TPLP+P VDTGMGLER+AAV+Q VHSNYEIDLF NLI A A++  + +L N SL VIADHIRS+AFLI DGV PSNEGRGYVLRRI+RRAIRHG++LG + PF   L+  L++EMG AYPELV+ +AH+E+ L +E ERF ETLD GL +L+  IA LS + IPG  +FKLYDTYGFPVDLT D+ARERG++LD+ GF+  M+ QR++AR+A+ F  K    ++    T F GY + S  AKII + V+ + V  L  G+ A++VL++TPFYAESGGQ GDKG + +  A F V DTQK A +VI HIG++  G +++ D V  QVND  R  T +NHSATHL+HAALR VLG+HV QKGSLV+++RLRFDFSH+EP++ +QL+++  +VN QIR N EV T  M ++EA   GA ALFGEKY ++VRV+ M DFS+ELCGGTHV RTGNIGLF I +ETG+AAGVRR+EA+T   A+ W +E   +L  ++ VLK++ D++  ++ QLL+++R LEK+ +QL++K
Sbjct:    1 MKSSAQLRQAFLDYFHEHGHEIVASSPLVPANDPTLLFTNAGMVQFKDVFLGLEERAIHRACSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIHFAWEFLTGVMGLSPEKLWVTVFEEDDEAADIWLKEVKVDPARFSRIGAKDNFWSMGDTGPCGPCSEIFYDHGEAVAGGPPGTPEADGDRYVEIWNLVFMQYDRGKDGTLTPLPRPSVDTGMGLERLAAVMQGVHSNYEIDLFRNLIKAGAELAGTDELSNTSLKVIADHIRSSAFLITDGVVPSNEGRGYVLRRIVRRAIRHGHKLGLREPFFFRLVTPLVAEMGAAYPELVRAQAHVERALRSEEERFAETLDQGLQILEHTIAGLSGEVIPGAAVFKLYDTYGFPVDLTADIARERGMSLDMAGFDREMAAQRERARAASQFEGKHAAKLNIDVPTEFTGYERLSDKAKIIGIYVEGQPVDVLQAGQAALVVLERTPFYAESGGQVGDKGTLSSDAATFVVRDTQKQAGEVITHIGELQSGTLRLGDHVSAQVNDSERRDTASNHSATHLMHAALRAVLGEHVTQKGSLVDADRLRFDFSHFEPVSREQLAEIEAMVNAQIRANHEVETKLMAVDEALASGAMALFGEKYGERVRVLRMSDFSVELCGGTHVGRTGNIGLFKIVAETGVAAGVRRVEAVTGPKALAWVNEAEQRLERVASVLKSSRDAVSDRVVQLLEQHRALEKEYEQLKAK 743          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1110754940|ref|WP_071979628.1| (MULTISPECIES: alanine--tRNA ligase [Alteromonas] >gi|1108514318|gb|APE05977.1| alanine--tRNA ligase [Alteromonas sp. RW2A1] >gi|1304128075|gb|AUC87692.1| alanine--tRNA ligase [Alteromonas sp. MB-3u-76])

HSP 1 Score: 977.237 bits (2525), Expect = 0.000e+0
Identity = 481/832 (57.81%), Postives = 607/832 (72.96%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKD----KQQLQSK-------NTVAIYVEKYGGTSVANVERINA-----VADNVERSHKLGHKLVVVLSAMSGETNRLLGLAKEIHPDAGAREIDVLVST 816
            +  + +R+KFI+YF S GH  VASSSL+P +DP+LLFTNAGM QFKD  LG DKR Y RAV+SQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK+EAIEFAW  LTKE+GLPK+KL +TV+ EDDEA DIW  HIGVPKE++IRI   DNFW+MGDTGPCGPCSEIFYDHGE + GGPPG+P+EDGDR+IEIWNLVFMQYN+ ADG M  LP P +DTGMGLERI+A+LQ+VHSNYEIDLF NLI +AA +  + DL++KSL VIADHIRS +FLI DGV PSNEGRGYVLRRIIRRA+RHGY+LG    F + L+ SL SEMGEAYPELV QK  +EK+L  E E+F +TL  G+ +L+  +  L    IPGD++FKLYDTYGFPVDLTNDVARE  L +D  GFE+ M +QR +A+ A++F +     +   + T F GYT+  GSA ++ L  D+  V TL  G++ V+VLD TPFYAESGGQ+GDKG+++ A  EF V DTQK   K   H G V KG +K +D V   ++   RE  + NHSATHLLHAALR +LGDHV QKGSLVE+ R+RFDFSH+E +++ Q++++   VN ++R N  +TT  M+LE+AK  GA ALFGEKY+++VRVV M  FS+ELCGGTHV RTG+IGLF I SE GIA+GVRRIEA+T + AI+    Q   L  LS +LKT+  ++  ++  L    +ELEK     KQ+L S+       NTV I   K     VAN+E + A     + D+++  +++G  +VV+  A   + N ++G+ K++     A E+   V++
Sbjct:    3 ISTADLRNKFIDYFKSHGHHAVASSSLVPADDPTLLFTNAGMNQFKDTLLGADKRSYNRAVSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKKEAIEFAWTFLTKEVGLPKEKLLVTVYSEDDEAFDIWENHIGVPKEKIIRISTSDNFWSMGDTGPCGPCSEIFYDHGEHIWGGPPGTPEEDGDRFIEIWNLVFMQYNKQADGSMESLPAPSIDTGMGLERISAILQNVHSNYEIDLFQNLIKSAADIVGTTDLEDKSLRVIADHIRSCSFLICDGVMPSNEGRGYVLRRIIRRAVRHGYKLGADDIFFYKLVASLASEMGEAYPELVDQKPVVEKVLRVEEEQFSKTLARGMAMLNDTLESLKGDTIPGDIVFKLYDTYGFPVDLTNDVAREHELKIDEAGFEAAMQEQRARAQQASNFGTDYNSRLVVEQETAFTGYTEVEGSATVVELIADNEFVSTLEDGQEGVVVLDNTPFYAESGGQSGDKGVLRVANGEFVVSDTQKMG-KAFAHRG-VAKGTVKKEDTVKAAIDIANREAIKKNHSATHLLHAALREILGDHVTQKGSLVEAPRMRFDFSHFESLSAQQIAEIERRVNAEVRANHALTTQLMELEDAKASGAMALFGEKYDEKVRVVTMGPFSVELCGGTHVERTGDIGLFKIVSEAGIASGVRRIEAVTGEYAIEMIQNQQTTLASLSAMLKTDPTNVVDRVSVLQSHTKELEKSLTAAKQKLASQQGADMLSNTVEINGVK---VLVANLEGVEAKSLRSMMDDIK--NRIGEGIVVLGVANESKVNLIVGVTKKLTGQVKAGELVNFVAS 827          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|653062533|ref|WP_027313852.1| (alanine--tRNA ligase [Balneatrix alpica])

HSP 1 Score: 976.467 bits (2523), Expect = 0.000e+0
Identity = 483/828 (58.33%), Postives = 602/828 (72.71%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKE--LGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIG-------AKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSF-VSKTQ-VHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDD----FSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSK------NTVAIYVEKYGGTSVANVERINAVADNVER------SHKLGHKLVVVLSAMSGETNRLLGLAKEI 801
            MK ++IR  F++YF SQGH+ VASS LIPGNDP+LLFTNAGMVQFKDVFLG D R Y+RA TSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQ+AI FAW  LT    + +PK+KLW+TV+  DDEA  IW   +GVP +R+IRIG       A DNFWAMGDTGPCGPC+EIFYDHGEDV GGPPGSPDEDGDRYIEIWN+VFMQYNR ADG M PLP+P VDTGMGLERI+AV+Q VHSNYEIDLF NL+ A A+VT + D  N SL VIADHIRS AFLI DGV PSNEGRGYVLRRI+RRAIRHG++LG  + F H L+  L++EMGEAYPEL + +A +E+++  E E+F +TLD G+ +L++ IA L  K I GD +FKLYDTYGFPVDLT D+ARERGL LD+ GFE  M  QR++AR+A+ F V  +Q + ++G   T F GY     + K++AL  D   V+ L+ G +AV+VLD+T FYAESGGQ GD G++  A   F V DT K   KV  H G++++GE+++D  V T+V+   R+    NHSATHL+H ALR +LG+HVQQKGSLV  +RLRFDFSH+E ++++QL QV  +VN+QIR N +V    M LEEAK  GA ALFGEKYED+VRV+ M +    FS ELCGGTHV RTG+IGLF I SE+G+AAGVRRIEA+T   A+ + D+    L  L+  LK N +S+  K E LL++NR LE++ +QL+ K      N +A    +  G  V  V ++  V     R       +KLG  +V++ +   G+ + + G+ KE+
Sbjct:    1 MKSAEIRQAFLDYFKSQGHEVVASSPLIPGNDPTLLFTNAGMVQFKDVFLGTDPRSYRRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQDAIRFAWEFLTSAQWMNIPKEKLWVTVYASDDEAYAIWRDEVGVPADRIIRIGDNKGAPYASDNFWAMGDTGPCGPCTEIFYDHGEDVFGGPPGSPDEDGDRYIEIWNVVFMQYNRTADGQMHPLPRPSVDTGMGLERISAVMQGVHSNYEIDLFQNLLRAVAEVTAAEDPSNHSLKVIADHIRSCAFLITDGVLPSNEGRGYVLRRIMRRAIRHGHRLGATSSFFHKLVAPLVAEMGEAYPELAKAQAQVERVILKEEEQFAKTLDQGMRILEQAIAELQSKVIAGDTVFKLYDTYGFPVDLTADIARERGLELDMPGFEQAMEAQRERARAASQFGVDYSQTLQVEG--QTEFCGYEHLQDAGKVMALLCDGNKVEQLSAGMEAVVVLDKTAFYAESGGQVGDSGVLSWAGGRFAVRDTTK-EGKVFLHHGRMLEGELQLDQVVQTEVDQARRQAIVLNHSATHLMHEALRRILGEHVQQKGSLVAPDRLRFDFSHFEAVSAEQLQQVEAMVNEQIRANQDVQVQLMQLEEAKAAGAMALFGEKYEDEVRVIRMGEGSGAFSTELCGGTHVRRTGDIGLFRIISESGVAAGVRRIEAVTGSEALRYVDQSEQALRQLASALKANRESVLSKAEALLERNRLLERELEQLKLKLASSAGNDLAAQAAEINGVKVL-VTKVEGVEPKALRDLLDQLKNKLGSAVVLLATESDGKVSLVAGVTKEV 824          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1130442732|ref|WP_075610547.1| (MULTISPECIES: alanine--tRNA ligase [Alteromonadales] >gi|1253242664|gb|PDM57843.1| alanine--tRNA ligase [Alteromonas pelagimontana])

HSP 1 Score: 973.385 bits (2515), Expect = 0.000e+0
Identity = 477/823 (57.96%), Postives = 602/823 (73.15%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEK----DKQQLQSKNTVAIY---VEKYG-GTSVANVERINA-----VADNVERSHKLGHKLVVVLSAMSGETNRLLGLAKEIHPDAGAREI 810
            +  ++IR  FI+YF   GH+ VASSSL+P +DP++LFTNAGM QFKDVFLG +KR Y RAV+SQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK++AI FAW  LT+EL LPK+KL +TVF EDDEA  IW   IGVP E++IRI   DNFW+MGDTGPCGPCSEIFYDHGE + GGPPGSP+EDGDR+IEIWNLVFMQYN+ ADG M PLPKP +DTGMGLERI+A+LQ+VHSNYEIDLF NLI A+A +  + DL+NKSL VIADHIRS +FL+ DGV PSNEGRGYVLRR+IRRA+RHGY++G    F + L+ +L  EMG+AYPELV Q+  +EK+L  E E+F +TL  G+ +L   +A L    IPGDVIFKLYDTYGFPVDLTNDVARE  L +D  GFE+ M  QR++A+ A+ F +     +     T F GY    G A++I L  D+R  + L+ G++ V+VL+QTPFYAESGGQ+GDKGI+K A  EF V DTQK  N    H G+V+ GEI+  D+    ++   RE  + NHSATHLLHAALR +LG+HV QKGSLVE++R+RFDFSH+E +T  QL+ +   VN++IR N  +TT  MDL+ AK  GA ALFGEKY+++VRVV M  FS+ELCGGTHVSRTG+IGLF IT+E+GIA+GVRRIEAIT  AAID+   Q   L+ +S +LKT+   +  ++  L  +N+ELEK     KQ+L S+    I    V+  G    V NVE + A     + D+++  +++G  +VV+  A   + N + G+ K++     A E+
Sbjct:    3 LSTAEIRQTFIDYFKKHGHQPVASSSLVPADDPTILFTNAGMNQFKDVFLGAEKRSYTRAVSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKKQAITFAWTFLTEELKLPKEKLLVTVFAEDDEAFAIWENDIGVPAEKIIRINTSDNFWSMGDTGPCGPCSEIFYDHGEHIWGGPPGSPEEDGDRFIEIWNLVFMQYNKQADGSMEPLPKPSIDTGMGLERISAILQNVHSNYEIDLFQNLIKASADIVGTDDLENKSLRVIADHIRSCSFLVCDGVMPSNEGRGYVLRRVIRRAVRHGYKMGANDIFFYKLVAALAKEMGQAYPELVDQQPVIEKVLRVEEEQFSKTLARGMAMLHDTLAELDGDTIPGDVIFKLYDTYGFPVDLTNDVAREHDLKIDEAGFEAAMQAQRERAQQASQFGANYNDMVKIEHQTQFTGYVDVEGEAEVIELIADNRLCEALSDGQEGVVVLNQTPFYAESGGQSGDKGILKVANGEFIVTDTQKLGN-AFAHKGKVV-GEIRKGDKASASIDKANREAVKKNHSATHLLHAALREILGEHVSQKGSLVEAQRMRFDFSHFEAVTDAQLTIIEQRVNEEIRANHSLTTELMDLDAAKASGAMALFGEKYDEKVRVVTMGPFSVELCGGTHVSRTGDIGLFKITTESGIASGVRRIEAITGAAAIDYIQAQQALLSRVSALLKTDTQGVLERVGALQSQNKELEKALSAAKQKLTSQQGADILGQAVDINGVKVLVTNVEGVEASSLRGMMDDIK--NRIGEGIVVLGVANESKVNLIAGVTKQLTSKVKAGEL 821          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|966690529|gb|KTG23293.1| (alanine--tRNA ligase [Idiomarina sp. H105] >gi|1026835163|gb|OAE90686.1| alanine--tRNA ligase [Idiomarina sp. WRN-38])

HSP 1 Score: 973.385 bits (2515), Expect = 0.000e+0
Identity = 456/766 (59.53%), Postives = 578/766 (75.46%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSKNTVAIYVEKYGGTSVANVERINAV 766
            M  +QIR  F+ YF  +GH+ V S+S+IPGNDP+LLFTNAGMV FKDVFLG DKR Y +A ++QRC+RAGGKHNDLENVGYTARHHTFFEM+GNFSFGDYFK++AIE+AW+ LT EL LP +KLW+TVF EDDEA DIW K IGVP++R+ RIG KDNFW+MGDTGPCGPCSE+FYDHG +V GGPPG+P+EDGDRYIEIWNLVFMQYNR ADG + PLP P +DTGMGLERI+A++Q+VHSNYEIDLF  LI +AAK+  + DL NKSL VIADHIRS +FLI DGV PSNEGRGYVLRRIIRRA+RHG+ LG +  F   L+D L+++MGEAYPELV++++ ++  L  E ++F  TL+ GL +L  EI  L    IPGDV+FKLYDTYGFPVDLT D+ARE+ LT+D  GFE  M++QR++A+ A+ F       I   + T F GY Q +G AK++ L  + +AV+ L  G+  ++VL +TPFYAESGGQTGD G +   +  F+V DTQ +  K IGH G+     I++ D V  +++   RE  + NHSATHL+HAALR +LG+HV QKGSLVE++++RFDFSH+EP++++Q++Q+   VN QIR N  + T  MD+EEAK  GA ALFGEKY++QVRVV M DFS+ELCGGTHV  TG+IGLF ITSE GIA+GVRRIE +T +AAI ++  Q   LN ++  +K   D++  KI+Q+LQK RELE+  +QLQ K        + GG  V+    I  V
Sbjct:    3 MNSAQIRETFLSYFAKRGHERVPSASMIPGNDPTLLFTNAGMVPFKDVFLGSDKRRYDKATSAQRCLRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFKKQAIEYAWDFLTNELKLPAEKLWVTVFTEDDEAYDIWAKDIGVPEDRISRIGEKDNFWSMGDTGPCGPCSEVFYDHGPEVWGGPPGTPEEDGDRYIEIWNLVFMQYNRQADGTLEPLPNPSIDTGMGLERISAIMQNVHSNYEIDLFQALIQSAAKIIGTDDLNNKSLRVIADHIRSCSFLIADGVMPSNEGRGYVLRRIIRRAVRHGHLLGAKETFFFQLVDELVNQMGEAYPELVEKQSIIKDALEREEQQFARTLERGLAILSDEIKQLDGTVIPGDVVFKLYDTYGFPVDLTADIAREQELTVDEPGFEKAMAEQRKRAQQASQFGMDYNEQIKSEQKTAFTGYEQVNGDAKVVELFKEGKAVERLEAGESGIIVLSETPFYAESGGQTGDTGKLVANSGVFEVTDTQ-YIGKAIGHHGKA-SATIQLGDSVKAEIDAERRENIKRNHSATHLVHAALRNLLGEHVTQKGSLVEADKMRFDFSHFEPVSAEQIAQIEREVNAQIRANLSLETQLMDIEEAKAAGAMALFGEKYDEQVRVVRMGDFSMELCGGTHVRSTGDIGLFRITSEGGIASGVRRIEVVTGEAAIRYTQGQQAMLNQVAAEIKAKPDNLIEKIQQVLQKQRELERQNEQLQKK-----LASQAGGGLVSQAAEIAGV 761          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1057249389|ref|WP_068619407.1| (alanine--tRNA ligase [Woeseia oceani] >gi|1041529027|gb|ANO53256.1| alanine--tRNA ligase [Woeseia oceani])

HSP 1 Score: 971.844 bits (2511), Expect = 0.000e+0
Identity = 457/745 (61.34%), Postives = 571/745 (76.64%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKD----KQQLQS 741
            M   ++R  F+E+F ++ H+ VASS L+PGNDP+LLFTNAGMVQFKDVFLG ++R+Y+RAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK++AI++AW  LT+ +GL  +KLW+TV+E+DDEA DIW+K +GV K R  R+GAKDNFWAMGDTGPCGPCSEIFYDHG DV GGPPGSPDEDGDRY+EIWNLVFMQ+ R ADG +TPLPKP VDTGMGLERIAAV+Q VHSNY+IDLF NLI+AAAK+   ++  + SL VIADHIR+ AFLIVDGV P NEGRGYVLRRIIRRA+RHG +LG +  F   L   LI+EMGEAYPEL + +AH+EK+L  E ERF ETLD G+ +L+  IA L  K+IPGDV+FKLYDTYGFPVDLT D+ARER LT+D +GFES M  QR +AR+A+ F +    ++     + F+GY   + + K+IAL +D + V+ L  G+  ++VLD TPFYAESGGQ GD G +  A A F V DT+K + K   H+G V  G I + DE+   V++  R     NHSATHL+HAALR +LG+HV QKGSLV  +RLRFDFSHYEP+T +QL ++  +VN++IR N       M  +EA   GA ALFGEKY+D VRV+ + DFS+ELCGGTHV+R+G+IG+F ITSE G+A+GVRRIEA+T Q A++W D     L+ ++ ++K+  +    K+EQL+++NRELEK     KQQL S
Sbjct:    1 MTSKELRQAFLEFFRARDHEVVASSPLVPGNDPTLLFTNAGMVQFKDVFLGEEQRNYQRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEQAIQYAWEFLTETMGLTPEKLWVTVYEDDDEAADIWLKKMGVDKNRFSRLGAKDNFWAMGDTGPCGPCSEIFYDHGADVPGGPPGSPDEDGDRYVEIWNLVFMQFERAADGTLTPLPKPSVDTGMGLERIAAVMQGVHSNYQIDLFANLINAAAKIVGVQNNGSSSLNVIADHIRACAFLIVDGVLPGNEGRGYVLRRIIRRAVRHGKKLGAEGTFFWKLAQPLIAEMGEAYPELAKAQAHVEKVLRKEEERFAETLDQGMEILENAIADLQGKEIPGDVVFKLYDTYGFPVDLTADIARERDLTVDTEGFESEMDAQRDRARAASKFAAGDSGNVRTEVESAFSGYDGLADNGKVIALFLDSQPVEMLKEGQAGIVVLDNTPFYAESGGQVGDTGTLGNAAASFVVNDTRK-SGKANLHVGTVASGSINVGDELHADVDEQRRRAIVLNHSATHLMHAALRKILGEHVTQKGSLVAPDRLRFDFSHYEPVTKEQLERIEAMVNEEIRQNTAAEVNLMGYDEAVASGAMALFGEKYDDVVRVLRLGDFSVELCGGTHVARSGDIGVFKITSEGGVASGVRRIEAVTGQGALEWIDRNQHSLDEVAGLVKSTREQAAVKVEQLVRRNRELEKQLAEAKQQLLS 744          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1011630823|ref|WP_062481189.1| (alanine--tRNA ligase [Lacimicrobium alkaliphilum] >gi|962032880|gb|ALS99194.1| alanine--tRNA ligase [Lacimicrobium alkaliphilum])

HSP 1 Score: 970.689 bits (2508), Expect = 0.000e+0
Identity = 454/742 (61.19%), Postives = 580/742 (78.17%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEKDKQQLQSK 742
            +  + IR KF +YF+S GH+ V SSSL+P +DP+LLFTNAGM QFKDV LG +KR Y RAV++QRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK+EAIEFAW+ LTKEL LP+ KL +T++ EDDEA DIW +HIGVP E++IRI   DNFW+MGDTGPCGPCSEIFYDHGE + GGPPG+P+EDGDR+IEIWNLVFMQYNR ADG + PLPKP +DTGMGLERI+A++Q VHSNYEIDLF  LI AAA++T ++DL++KSL V+ADHIRS +FLI DGV PSNEGRGYVLRRIIRRA+RHGY+LG Q  F + L+D+LI +MGEAYPEL  Q++ +E++L  E E+F  TL+ G+ +L + +  L+DK IPG+++FKLYDTYGFP DLTNDVARE+G ++D  GFE  M++QR++A+ A++F       +    ++ F GY Q SG A+I+ L   D  V+ L  G+Q +++LD+TPFYAE+GGQ GD G +K A   FK+ DT K  +  I H G + +  IK+ D+   Q++   R+  + NHSATHLLHAAL+ VLG+HV QKGSLV+ ERLRFDFSH+E +++ Q++++  +VNQQIR N  +TT  MDLEEAK  GAQALFGEKY+DQVRVV+M DFS+ELCGGTHVS+TG+IGLF ITSE GIA+GVRRIEA+T   A+ +S  Q   L  ++  LK++   +  +I QL++++R LEK+ QQL+ K
Sbjct:    3 ISTADIRRKFFDYFSSHGHQLVPSSSLVPADDPTLLFTNAGMNQFKDVLLGAEKRSYNRAVSAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKKEAIEFAWHFLTKELKLPEQKLLVTIYAEDDEAFDIWSQHIGVPAEKIIRISTSDNFWSMGDTGPCGPCSEIFYDHGEHIWGGPPGTPEEDGDRFIEIWNLVFMQYNRQADGTLEPLPKPSIDTGMGLERISAIMQDVHSNYEIDLFQALIKAAAEITGAKDLEDKSLRVVADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGAQDIFFYRLVDALIGQMGEAYPELKTQRSTIERVLKVEEEQFSRTLERGMQILQEALESLTDKTIPGELVFKLYDTYGFPADLTNDVAREKGFSIDEAGFEKAMAEQRKRAQQASNFGVDYNSQLSSYSSSEFTGYDQQSGEARILELFSTDSKVQQLESGEQGMVILDRTPFYAEAGGQVGDTGYLKLANGGFKITDTIKLGS-AIAHKG-IAEAPIKVGDKASLQIDAERRQAIKLNHSATHLLHAALKNVLGEHVNQKGSLVDPERLRFDFSHFEGVSAGQITEIEDLVNQQIRLNHPLTTKLMDLEEAKSAGAQALFGEKYDDQVRVVSMGDFSMELCGGTHVSQTGDIGLFRITSEGGIASGVRRIEAVTGAQALKFSQHQSQLLCDVAAQLKSDWHKLPERIGQLVEQSRNLEKEVQQLKQK 742          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|764659822|ref|WP_044446526.1| (alanine--tRNA ligase [Alteromonas australica] >gi|761522919|gb|AJP43240.1| alanyl-tRNA synthetase [Alteromonas australica])

HSP 1 Score: 970.689 bits (2508), Expect = 0.000e+0
Identity = 471/823 (57.23%), Postives = 605/823 (73.51%), Query Frame = 0

Query:    1 MKVSQIRSKFIEYFNSQGHKEVASSSLIPGNDPSLLFTNAGMVQFKDVFLGFDKRDYKRAVTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIEFAWNLLTKELGLPKDKLWITVFEEDDEAEDIWVKHIGVPKERVIRIGAKDNFWAMGDTGPCGPCSEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYNRDADGVMTPLPKPCVDTGMGLERIAAVLQHVHSNYEIDLFVNLISAAAKVTNSRDLKNKSLAVIADHIRSTAFLIVDGVTPSNEGRGYVLRRIIRRAIRHGYQLGCQTPFMHLLIDSLISEMGEAYPELVQQKAHLEKILYNENERFLETLDLGLGLLDKEIAVLSDKQIPGDVIFKLYDTYGFPVDLTNDVARERGLTLDLDGFESCMSQQRQQARSANSFVSKTQVHIDGIENTLFNGYTQTSGSAKIIALQVDDRAVKTLTIGKQAVLVLDQTPFYAESGGQTGDKGIIKTATAEFKVCDTQKFANKVIGHIGQVIKGEIKIDDEVVTQVNDITREQTQNNHSATHLLHAALRVVLGDHVQQKGSLVESERLRFDFSHYEPITSDQLSQVGHIVNQQIRHNCEVTTTQMDLEEAKEKGAQALFGEKYEDQVRVVAMDDFSLELCGGTHVSRTGNIGLFSITSETGIAAGVRRIEAITAQAAIDWSDEQHLKLNHLSQVLKTNVDSIEHKIEQLLQKNRELEK----DKQQLQSKNTVAIY--VEKYGGTSV--ANVE-----RINAVADNVERSHKLGHKLVVVLSAMSGETNRLLGLAKEIHPDAGAREI 810
            +  + +R KFI+YF S GH+ VASSSL+P +DP+LLFTNAGM QFKD FLG ++R Y RAV+SQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK+EAIEFAWN LTKE+ LPK+KL +TV+ EDDEA DIW  HIGVPKE++IRI   DNFW+MGDTGPCGPCSEIFYDHG  + GGPPG+P+EDGDR+IEIWNLVFMQYN+ ADG M PLPKP +DTGMGLERI+A+LQ+VHSNYEIDLF NLI A+A +  ++DL+NKSL VIADHIRS +FLI DGV PSNEGRGYVLRRIIRRA+RHGY++G Q  F + L+++L  EMG AYPELV Q+  +EK+L  E E+F +TL  G+ +L+  +  L    IPGDV+FKLYDTYGFP DLTNDVARE  L +D  GFE+ M  QR++A+ A+SF +     +     T F GYT+  G A ++ L  D+    +L  G++ V+VLDQTPFYAESGGQ+GDKG+++ A  EF V DTQK  N    H G+V KG I   D+    +++  RE  + NHSATHLLHAALR +LG+HV QKGSLVE++R+RFDFSH+E +T++Q++QV   VN+++R N  + T  M+L+EAK  GA ALFGEKY+++VRVV M  FS+ELCGGTHV  TG IGLF I +E GIA+GVRRIEA+T + AI +  +Q   L+ +S +LK++V ++  +I  L    +ELEK     KQ+L S+    +   V +  GT V  AN+E      + ++ D+++  +++G  +VV+  A   + N + G+ K +     A E+
Sbjct:    3 LSTADLRQKFIDYFKSHGHQAVASSSLVPADDPTLLFTNAGMNQFKDTFLGTEQRSYTRAVSSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKKEAIEFAWNFLTKEIQLPKEKLLVTVYSEDDEAFDIWENHIGVPKEKIIRISTSDNFWSMGDTGPCGPCSEIFYDHGAHIWGGPPGTPEEDGDRFIEIWNLVFMQYNKQADGTMEPLPKPSIDTGMGLERISAILQNVHSNYEIDLFQNLIKASADIVGTQDLENKSLRVIADHIRSCSFLICDGVMPSNEGRGYVLRRIIRRAVRHGYKMGAQDIFFYKLVEALSKEMGAAYPELVDQRPVIEKVLRVEEEQFSKTLARGMAMLNDTLDALEGDTIPGDVVFKLYDTYGFPTDLTNDVAREHDLKIDEAGFEAAMQAQRERAQQASSFGADYNSKLAVEHQTSFTGYTEVEGEANVVELIADNAFCDSLADGQEGVVVLDQTPFYAESGGQSGDKGVLRVAKGEFIVTDTQKMGN-AFAHKGKV-KGVINKGDKASAAIDNSNREAVKKNHSATHLLHAALREILGEHVTQKGSLVEAQRMRFDFSHFEAMTTEQIAQVERRVNEEVRANHVLKTELMELDEAKASGAMALFGEKYDEKVRVVTMGPFSVELCGGTHVKSTGEIGLFKIVTEAGIASGVRRIEAVTGEHAIAYVQKQQSMLSEVSGLLKSDVQNVVERIISLQNHTKELEKALNSAKQKLASQQGADMLNSVIEIKGTKVLIANLEGVESKSLRSMMDDIK--NRIGEGIVVLGVAGDNKVNLIAGVTKGLTSKVKAGEL 821