af190129_1na+ k+ 2cl- cotransporter, maker-scaffold1253_size52701-snap-gene-0.12 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000003059 (supercontig:LSalAtl2s:LSalAtl2s1734:14347:19068:1 gene:EMLSAG00000003059 transcript:EMLSAT00000003059 description:"maker-LSalAtl2s1734-augustus-gene-0.5") HSP 1 Score: 1278.46 bits (3307), Expect = 0.000e+0 Identity = 672/1127 (59.63%), Postives = 851/1127 (75.51%), Query Frame = 0
Query: 66 GECPSGSDPSFPD---VPECLTPSPYLP---TRNGN--------RKRSSVTIA-PGTILEKPLPSQRKMTSKQRSSFILTPKPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVER-INDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGL----ASPATLTKRKLGRDGSVLGR--------------FQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNK-SIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMI---TIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
G PSG D +F + + + ++ YL R+ N R+R SVTI+ P EKP PS R+++ K+ S T + L+HYLT+E +PH DHYRN++SF+KG P+ SRPTLD+LL++ S N +D E + +D G IKFGW +GVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGL IITLSNIVTVIT+ISMSAVSTNGQIKAGGIY+MISRSLGPEFGGAIG+MFTLANSIAV+MYIIGFCE+L DML+QY+ F GI+++ E ++D+RLIGS+SLVL+L LAIVGMEWVTRVQ GLL+LLL SQ+DFIIGS +P S++++AKGFVGY+ VL NL++ Y +E + ++FF+VFAVFFPAVTGIVAGANLSGDLKDP VAIPKGTLL IV+TYVTY IYG+M+A + R ASG+V E+ D N+T+ E+ NIT ++ +CE R C+YGIL SQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRV QAVAKDKLFPGI SF++GWG NNDPV+GY+LVF+ISLICILIGDLN VSSLLSNFFVAAYALINFSVFHASIT SPGWRP FKYYNKWISLLGT+LCI VMFLMD TA++T + + LY+++ RKP+VNWGSS+Q+QSF+TAL +V +LT+V+DHVKNYRPKIMV+SG+P++RP+L+DFA+L+TKR+SLLES+ + LD+KK A+K++ W NHIK F S+ RN + EGAR A+E+ GLGKL NM+L+GF+ W + A+EYY+ ++TAFD+RLAVGVLRI+GGLD++ M E +S +LN++D ES MSVDSGL S A T + G D G F+KK SS+ L +KD +P++E+ V +++QFR+K ++ +GTID+YWLYDDG HIL TR K++K K+R+FFLS DELD TR+M +LL+KFRI+F DVI+L D TK+ K+T+D+FKAM+ ++ + SG+ +S + + + D + LV+E ++ + E+TNF LRIAEIV+ NSS ADL+VMTLP+PK+E +P LYMAWLD+TT+ MPPFL+VRGNQ SVLT
Sbjct: 27 GGDPSGGDDNFSENGSIDDSISYKNYLAYELNRSSNPGGGGGGDRRRPSVTISVPEN--EKPSPSSRRLSRKRSSFQFTTQRSLRHYLTREAIPHADHYRNKMSFAKGKPDSSRPTLDDLLNDDPYSKDN-SDLE---SQTDDQDTGSVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLAIITLSNIVTVITSISMSAVSTNGQIKAGGIYFMISRSLGPEFGGAIGLMFTLANSIAVAMYIIGFCEALSDMLKQYIPGFIGILNTPEDGLHDIRLIGSVSLVLLLGLAIVGMEWVTRVQKGLLVLLLFSQLDFIIGSFLPPSEDKLAKGFVGYNGTVLMENLFSEYIPNEGTEKNNSFFTVFAVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLXIVSTYVTYTIYGVMIAAXTXREASGDVLEL----DYVNETIKEVNNITNSFWECENRTCSYGILHSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVFQAVAKDKLFPGIASFADGWGKNNDPVKGYLLVFLISLICILIGDLNYVSSLLSNFFVAAYALINFSVFHASITKSPGWRPGFKYYNKWISLLGTVLCICVMFLMDMVTALVTFIIVFFLYMYVSIRKPEVNWGSSTQAQSFMTALNSVLSLTKVEDHVKNYRPKIMVMSGSPLNRPTLIDFANLVTKRISLLESVDVVPEDLDWKKSQAIKNSGQNWFTENHIKGFFSVVRNSKISEGARTAMELSGLGKLRTNMILLGFQDTWWKKDESAKEYYKIIQTAFDLRLAVGVLRINGGLDVTSMTNLMED----------SSPNQSNALNYDDRCESNMSVDSGLDLATKSGAKNTSTQSGEDDKEDGSLKRNKKPNGFEKLLFKKKCPSSIPLPPSEKDVKPLDEDIVNRLSQFRDKTTVNTGTIDVYWLYDDGXXXX---HILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVP-PLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLIVMTLPMPKKEIVPQELYMAWLDYTTKDMPPFLLVRGNQTSVLT 1129 HSP 2 Score: 1021.92 bits (2641), Expect = 0.000e+0 Identity = 548/1053 (52.04%), Postives = 717/1053 (68.09%), Query Frame = 0
Query: 1120 REQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPA-RFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLR-LFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELY---------FQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNV-------DSFGTSSTRNNSEVSY-----------------GIRDGNPIDERIVYRMTQFRDKSQ-KVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGD---------------------EQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RE IP+ D KM +G DS SRPTLD+LL N Y + +S T D +DTG +FGWI+GVY+ CLLNIWGVMLFLRLTWV+GQAGLL AIIT++N+VT +T+ISMSAV TNGQIK GGIYFMISRSLGPEFGGA+GLMFT A SIAV+MYIIGFCEAL DM+ Q+ F GI+ E +D+R++GS +LVLLLG++ +GM V ++Q LL++LL SQ+DF+IG+F+PPS++K A G VG++ +L NL+ ++ N E+ F VFAVFFPAVTG+VAGAN+SGDLKDPG AIPKGTL I++TY +Y +Y ++IAA R ASG+ EL D +E F C NR C+YG L +QQM+++ S WGPLIYAGCFAATLSSA+ASLVG PRVFQA+AKDKLFP I SF G+G++ +P++GYLL+F I+L CILIGDLN VSSLLSNFFVA YA+IN+SVFHAS SPGWRP FKY+NKWISLLGT LCI VM LMD+ TAL T I + LY++V R P+VNWGSS+Q+QSF+TAL S+ +L VEDHVKNYRPK++V++G+P +RP L DFANL+TKR SLLE V V+ ED++ + +K +W +NHIK F+++ RN G RTA+ELSGLGKL NM+++GFQ+ WW + +EYY+++ +FD+ L+VGVLR+ GGLD++ ++ E ++ +Q++ + +DR +S+D + + +KN D S RN + +D P+DE IV R++QFRDK+ GTIDVYWLYDDG +IL TR K++KCKMRVFFLSN+ D L + MASLL KFRI+F+DVI+L+D TK PKKET F+ M++ + E P+VSE++++ + ++T F+LRIAEI K+NS + L+VMTLP+P++ +P LY+AWLD+ ++ MPPFL VRGNQ SVLTFYS
Sbjct: 114 REAIPHA------DHYRNKMS---FAKGKPDS---SRPTLDDLL----NDDPYSKDNSDLESQTDD--------QDTGSVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLAIITLSNIVTVITSISMSAVSTNGQIKAGGIYFMISRSLGPEFGGAIGLMFTLANSIAVAMYIIGFCEALSDMLKQYIPGFIGILNTPEDGLHDIRLIGSVSLVLLLGLAIVGMEWVTRVQKGLLVLLLFSQLDFIIGSFLPPSEDKLAKGFVGYNGTVLMENLFSEYIPNEGTEKNNSFFTVFAVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLXIVSTYVTYTIYGVMIAAXTXREASGDVLEL--DYVNETIKEVNNITNSFWECENRTCSYGILHSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVFQAVAKDKLFPGIASFADGWGKNNDPVKGYLLVFLISLICILIGDLNYVSSLLSNFFVAAYALINFSVFHASITKSPGWRPGFKYYNKWISLLGTVLCICVMFLMDMVTALVTFIIVFFLYMYVSIRKPEVNWGSSTQAQSFMTALNSVLSLTKVEDHVKNYRPKIMVMSGSPLNRPTLIDFANLVTKRISLLESVDVVPEDLDWKKSQAIKNSGQNWFTENHIKGFFSVVRNSKISEGARTAMELSGLGKLRTNMILLGFQDTWWKKDESAKEYYKIIQTAFDLRLAVGVLRINGGLDVTSMTNLMED--SSPNQSNALNYDDR---CESNMSVDSGLDLATKSGAKNTSTQSGEDDKEDGSLKRNKKPNGFEKLLFKKKCPSSIPLPPSEKDVKPLDEDIVNRLSQFRDKTTVNTGTIDVYWLYDDGXXXX---HILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVPPLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLIVMTLPMPKKEIVPQELYMAWLDYTTKDMPPFLLVRGNQTSVLTFYS 1132
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000009267 (supercontig:LSalAtl2s:LSalAtl2s58:680408:685072:1 gene:EMLSAG00000009267 transcript:EMLSAT00000009267 description:"augustus_masked-LSalAtl2s58-processed-gene-6.1") HSP 1 Score: 514.998 bits (1325), Expect = 5.586e-159 Identity = 336/695 (48.35%), Postives = 429/695 (61.73%), Query Frame = 0
Query: 113 LPSQRKMTSKQRSSFILTP-----KPLKHYLTKEVLPHVDHYRNRLSFSKGHPER-SRPTL----------DELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRK----CNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQ----VSSLLSNFFVAAYALI-----------NFSVFHASITSSPGWRPSF-KYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKV-NWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVL--SGNPVDRPSLMD-FAHLLTKRLSLLESIQI--TEASLDFKKV 765
+P K ++ +SF T K + YLT++ LP HYRN LS S HP + SRPTL LLD+ + D+ N+ E+ S+GK ++FGW +GV+MRCLLNIWGVMLFLRLTWV+GQ GL++GLG+I L N+VTVITT+SMSAVSTNGQI+ GGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFC+SL+DM +QY + ++ S + NDVR+IG+ISLV +LALAIVGMEWVTRVQM LL LL+ SQ+DF++GS +P+ E+ A GFVGY+ + NL Y + G +FFSVF VFFPAVTGIVAGANLSGDLKDP VAIPKGTLLAI+ T++TY+ YG ++ ASGN E + N G + +++C GR C YG QQ ME IS G LIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+P IH F+ G+GANNDP+RGY LVFIIS+ CILI +Q V L FV I N+ T P RP + N L ++C V + A + ++ V ++FR K+ ++ S +Q+++F A L + RP +++ G+ ++ P +D + +L L + I E+ DF KV
Sbjct: 36 VPDHDKAGGEETNSFHPTYSTQYFKSFRQYLTRDALPSESHYRNLLSISN-HPRKFSRPTLEELQEEQAENGRLLDKKDMDDVEKNE--------EEQSKGKVVRFGWIEGVFMRCLLNIWGVMLFLRLTWVVGQGGLIQGLGVIALGNLVTVITTLSMSAVSTNGQIRGGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCQSLLDMCKQYF-ELEHLIFS-DTTNDVRIIGAISLVAVLALAIVGMEWVTRVQMILLFLLIGSQIDFVVGSFLPTK-EDAAYGFVGYNGTLFVDNLSPTYHDYKDPGNPPSFFSVFGVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLAIIVTFITYIGYGTIITGVMHTEASGNQTEYE---NWINGVEGALY-----FNNCSGRPADDFCQYGSSNDQQAMERISYTGYLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIHIFAPGFGANNDPIRGYFLVFIISMGCILIATESQRLTGVVQLKXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHV----NIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLA----GIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKV 702 HSP 2 Score: 419.853 bits (1078), Expect = 1.735e-125 Identity = 238/463 (51.40%), Postives = 304/463 (65.66%), Query Frame = 0
Query: 1185 DCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVD-GEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEEL---LLDREDELYFQNCSNRN----CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGD----LNRVSSLLSNFFVATYAVINYSVFHASWNNSP----GWRPSFKYF 1631
D + + + RFGWI+GV++ CLLNIWGVMLFLRLTWVVGQ GL+ +I + NLVT +T +SMSAV TNGQI+GGGIY+MISRSLGPEFGGA+G+MFT A SIAVSMYIIGFC++LLDM Q+ I D+ NDVRI+G+ +LV +L ++ +GM V ++Q++LL +L+ SQ+DF++G+F+ P+ E A G VG++ L NL P + D G F VF VFFPAVTG+VAGAN+SGDLKDPG AIPKGTL AI+ T+ +Y+ Y +I ASGN E + E LYF NCS R C YG+ +QQ ++ S G LIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P+I F GFG + +P+RGY L+F I++ CILI L V L FV T I S N++P +RP F
Sbjct: 116 DVEKNEEEQSKGKVVRFGWIEGVFMRCLLNIWGVMLFLRLTWVVGQGGLIQGLGVIALGNLVTVITTLSMSAVSTNGQIRGGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCQSLLDMCKQYFELEHLIFSDT---TNDVRIIGAISLVAVLALAIVGMEWVTRVQMILLFLLIGSQIDFVVGSFL-PTKEDAAYGFVGYNGTLFVDNLSPTYHDYKDPGNPPSFFSVFGVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLAIIVTFITYIGYGTIITGVMHTEASGNQTEYENWINGVEGALYFNNCSGRPADDFCQYGSSNDQQAMERISYTGYLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIHIFAPGFGANNDPIRGYFLVFIISMGCILIATESQRLTGVVQLKXQQFVTTLKSIQ------SLNDTPEHVKNYRPKVLVF 568 HSP 3 Score: 345.895 bits (886), Expect = 2.637e-100 Identity = 185/433 (42.73%), Postives = 263/433 (60.74%), Query Frame = 0
Query: 1683 QSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+ Q F+T LKS+Q+LN +HVKNYRPK++V TG P+ RP L DFANLITK+ SLL HV D + ++ ++ V W RD+ IK+FY+LT+N +F G + L+G+GK+ NM++ G++ W PK +EY VL +FD+HL VG+LR++ G D S + G E+ DE Q KS++L K V R S Y DGNP+ + V +TQF+ K +K G IDV+WLYDDGGLTLLLPYIL+TR +FA+C++RVF L+NR D L E MA LL KFRI + V ++ DVTK T F+ ++ DE +P + E ++ ++T HLR+ E+ +E+S S +++MTL +PR+ LY++WL+ ++++MPPFL +RGNQ SVLTFYS
Sbjct: 537 KXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHVNIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLAGIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKVIGEEEQQQHEIELDEENEDEKNGK-----------QGKSLELQQKKV------RXRKVSTAVYTGIDGNPLPKNTVQDITQFQIKKRK-GNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKIL--DE---IKPPLEESEIISQKEKTNRHLRLTELLREHSKRSEMIIMTLSIPRRGTAVAQLYMSWLEMMTKNMPPFLLIRGNQSSVLTFYS 946 HSP 4 Score: 337.421 bits (864), Expect = 2.045e-97 Identity = 183/462 (39.61%), Postives = 271/462 (58.66%), Query Frame = 0
Query: 695 QSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKS-SMSLAILDK-DGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
+ Q F+T LK++Q+L +HVKNYRPK++V +G P RP L+DFA+L+TK+LSLL + FK ++ L++ W + IKSF+S+T+N + EGA A + + G+GK+ PNMVL G+K DW + +EY L AFD+ L VG+LR++ G D S++ +E+ Q EL E++ ED K G+ G L QKK + +S A+ DG P+ + TV+ ITQF+ K R G ID++WLYDDGGLT+LLP+ILTTR +FA+ +LR+F L+ DELD +TRNM LL+KFRI + V ++ D+TKKA T+++FK ++ P + E E+ E+TN LR+ E++R++S +++++MTL +P+R LYM+WL+ T+ MPPFL++RGNQ SVLT
Sbjct: 537 KXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHVNIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLAGIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKVIGEEEQQQ-------HEIELDEEN---ED--------------------EKNGKQGKSLELQQKKVRXRKVSTAVYTGIDGNPLPKNTVQDITQFQIKK-RKGNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKILDEIKPP------------------------LEESEIISQKEKTNRHLRLTELLREHSKRSEMIIMTLSIPRRGTAVAQLYMSWLEMMTKNMPPFLLIRGNQSSVLT 943
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000003697 (supercontig:LSalAtl2s:LSalAtl2s1:1949199:1954526:1 gene:EMLSAG00000003697 transcript:EMLSAT00000003697 description:"maker-LSalAtl2s1-augustus-gene-19.17") HSP 1 Score: 278.1 bits (710), Expect = 2.704e-78 Identity = 200/665 (30.08%), Postives = 337/665 (50.68%), Query Frame = 0
Query: 191 AEDSSEGKAIK-FGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMD-------MLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSS-----DEEIAKGFVG-----YDTAVLESNLYNAYT---KDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCE--GRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMD-FKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLN-SNH--IKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLAD 828
AED G+ GWF G + L+++ +LFLR+ +++G AG+L L I ++ + V T +S+SA++TNG +K GG+Y+MISR+LGPEFGG+IG++F AN + ++Y + E ++ L YL + + + R+ LI +L L++ ++G + + +L+ ++ + I S+ +E I+ F G + +S+LY +Y+ D+T V NF VFAV F VTGI+AGANLSGDLK+P+ +IPKGTLLA T++TY+I + +T D E R C+Y M+E S W P + G AT ++ + L+G+ RVL+A++KD +F + N+PV ++ +++S I IG +N ++ ++ F+ +YA +N S + S+P +RP F+ ++ + LG I + +MF+++ +A++ CI + K NWGS SQ+ F K + L DHVK +RP++++L NP + SL+DFA+ L K+ L + E + + + D WLN +H +K+F +T ++ G + + I G+G + PN ++ GF + + D
Sbjct: 11 AEDEHVGRGSGGLGWFKGXFAPVSLSMFSSLLFLRVGYILGNAGILHSLLQIVIAYGILVFTILSVSAIATNGAVKGGGVYFMISRTLGPEFGGSIGVLFYAANIASSALYAVACTECIISSFGEESGTLAHYLPE-SFWYQILYRV----LIN----LLNLSICLIGPNLFGKTTIMVLISVVFCTLTVITSFFSASTVEVHFNETISGSFTGILSKNWSIENFQSHLYPSYSLDCNDDTKPV--NFAIVFAVLFSGVTGIMAGANLSGDLKEPSKSIPKGTLLASFFTFITYVI---------------------------------LFTLTAMTCDKELLLRDCSY-------MIE-FSFWKPFVIIGTVLATFCASTSCLIGSSRVLEAISKDTMFGIFLEWITYGTVGNNPVLAVVVTWLLSNIFFFIGGVNTIAQFVAVLFLLSYASVNLSCLSLDLASAPNFRPIFRCFSWYTCALGLIGTLTMMFMINPVISALVLFACIFFFLSLSFLSPTKENWGSISQALLFHQVRKYLLLLDSRKDHVKFWRPQVLLLVSNPRNHCSLIDFANAL-KKGGLYVLGHVREGNFEGMEEDECGLLMPHWLNLVDHLKVKAFVELTVAPTVRSGIQQLIRISGIGAMKPNTIIFGFPYEDENMCKD 622 HSP 2 Score: 271.937 bits (694), Expect = 3.037e-76 Identity = 189/627 (30.14%), Postives = 320/627 (51.04%), Query Frame = 0
Query: 1197 GPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKR--------AMGIV--GFDPDLLRSNLWPDFQ--KNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFP-YIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMD-LGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLR-DNH--IKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGF 1806
G GW +G + P L+++ +LFLR+ +++G AG+L S I IA + T +S+SA+ TNG +KGGG+YFMISR+LGPEFGG++G++F A + ++Y + E ++ + S + +S R+L + +L L I IG L K +++L+ ++ + + F + E GI+ + + +S+L+P + N D + + VFAV F VTG++AGAN+SGDLK+P +IPKGTL A T+ +Y++ + A + +ELLL R+C+Y MI+ S W P + G AT ++ + L+G RV +AI+KD +F ++E G P+ ++ + ++ IG +N ++ ++ F+ +YA +N S ++P +RP F+ F+ + LG + +M +++ + +AL CI NWGS SQ+ F K L L+ +DHVK +RP+++++ NP + +L DFAN + K+ L L HV + + E D + HWL +H +KAF LT + G++ + +SG+G + N ++ GF
Sbjct: 19 GSGGLGWFKGXFAPVSLSMFSSLLFLRVGYILGNAGILHSLLQIVIAYGILVFTILSVSAIATNGAVKGGGVYFMISRTLGPEFGGSIGVLFYAANIASSALYAVACTECIISSFGEESGTLAHYLPESFWYQILYRVLIN---LLNLSICLIGPNLFGKTTIMVLISVVFCTLTVITSFFSASTVEVHFNETISGSFTGILSKNWSIENFQSHLYPSYSLDCNDDTKPVNFAIVFAVLFSGVTGIMAGANLSGDLKEPSKSIPKGTLLASFFTFITYVILFTLTAMTC-------DKELLL-------------RDCSY-------MIEF-SFWKPFVIIGTVLATFCASTSCLIGSSRVLEAISKDTMFGIFLEWITYG-TVGNNPVLAVVVTWLLSNIFFFIGGVNTIAQFVAVLFLLSYASVNLSCLSLDLASAPNFRPIFRCFSWYTCALGLIGTLTMMFMINPVISALVLFACIFFFLSLSFLSPTKENWGSISQALLFHQVRKYLLLLDSRKDHVKFWRPQVLLLVSNPRNHCSLIDFANAL-KKGGLYVLGHVREGNFEGMEEDECGLLMPHWLNLVDHLKVKAFVELTVAPTVRSGIQQLIRISGIGAMKPNTIIFGF 612
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000004234 (supercontig:LSalAtl2s:LSalAtl2s222:1210791:1213007:1 gene:EMLSAG00000004234 transcript:EMLSAT00000004234 description:"maker-LSalAtl2s222-snap-gene-12.7") HSP 1 Score: 217.624 bits (553), Expect = 6.899e-60 Identity = 172/614 (28.01%), Postives = 290/614 (47.23%), Query Frame = 0
Query: 256 SAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISL-VLILALAIVGMEWVTRVQMGLLLLLLVSQV--------------DFIIGSVIPSSDEEIAK------------------GFVGYDTAVLESNLY-NAYTKDE--TSGVQH--NFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQM-MEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKP-----KVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR-LSLLESIQITEASLDFKKVDALKSAANEWL---NSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQD 821
SA+S+NG ++ GG+Y+M+SR+LGPEFGGAIG +F AN ++ ++Y+ G E L+ + + R + + S S+ L L + +VG ++ + + + ++LV + DFI + P + +K G ++ +L N Y N Y K E SG NFF +F V F VTGI++GAN+ G+L P+ +IP+GT+ A T V Y+I ++ + C+ +LQ M M +++ P + G F AT S+++ +L+G R+L+A+AKD +F + +F +PV I + + + +++G LN ++ L S F+ +Y IN + + S+P +RP++KY++ + L+G + I +MF + +AI + V I+L I NWGS SQ+ F K + L + HVK +RP++++L NP SL++F + L K L +L + + +L+ D + WL + +K+F +T SL G + I G+G + PN VL+GFK D
Sbjct: 16 SALSSNGAVEGGGVYFMLSRTLGPEFGGAIGTLFFFANVVSSALYLAGCTEGLVSNFK-----------FLARGPWIEFLYSTSINSLNLFICLVGSKFFGKTSVLIFFMVLVCVLMTSVSFFLDYTITEDFIFNATDPLKCVQPSKNDTNQTPNCTLTSTGKFTGIGSWNETILVQNFYDNLYPKYEYDCSGSDKSLNFFIIFGVLFSGVTGIMSGANMCGELVSPSKSIPRGTISACGFTLVIYIILTVLTSL----------------------------------------TCSPTLLQHDCMYMNIVNVLPPAVTIGVFMATFSASLNNLIGXSRILEAIAKDVVFGPLFNFVLKGTYKENPVTAVISTWFVVELFLMMGSLNNIAQLCSVLFLLSYGTINLTCLFLHLASAPNFRPTYKYFSTYTCLIGLLGSIIMMFAV---SAIYSAVGILLCLSLILLLNLFSPIRHTNWGSISQALIFHQVRKYLLLLDQRKSHVKFWRPQMLLLVSNPRRNCSLIEFGNALKKSGLYVLGHVHVD--NLEEHDKDPTLNEIPHWLKLIDFLKVKAFVEVTIARSLRLGVDQLVRISGIGAMKPNTVLLGFKDD 573 HSP 2 Score: 214.927 bits (546), Expect = 5.054e-59 Identity = 163/592 (27.53%), Postives = 287/592 (48.48%), Query Frame = 0
Query: 1255 SAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTF--------------VPPSDEK-------------RAMGIVGFDPDLL----RSNLWPDFQKNVDG--EQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNP--DVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLR---DNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQE 1808
SA+ +NG ++GGG+YFM+SR+LGPEFGGA+G +F A ++ ++Y+ G E L+ ++F ++ I N + + + L+G F G V ++L+ VL+ S FL T V PS + GI ++ +L NL+P ++ + G + L F +F V F VTG+++GANM G+L P +IP+GT++A C + + + I + + + +P L +C Y + N + P + G F AT S++L +L+G R+ +AIAKD +F + +F + P+ + + + +++G LN ++ L S F+ +Y IN + ++P +RP++KYF+ + L+G I++M + + ++ + L + + +P NWGS SQ+ F K L L+ + HVK +RP+++++ NP +L +F N + K+S L L HV +++E D + HWL+ +KAF +T S GV V +SG+G + N +++GF++
Sbjct: 16 SALSSNGAVEGGGVYFMLSRTLGPEFGGAIGTLFFFANVVSSALYLAGCTEGLVSN-FKFLARGPWIEFLYSTSINSLNLF-----ICLVGSKFFGKTSVLIFFMVLVCVLMTSVSFFLDYTITEDFIFNATDPLKCVQPSKNDTNQTPNCTLTSTGKFTGIGSWNETILVQNFYDNLYPKYEYDCSGSDKSLNFFIIFGVLFSGVTGIMSGANMCGELVSPSKSIPRGTISA-----CGFTL-VIYIILTVLTSLTCSPTLL--------------QHDCMYMNIVN--------VLPPAVTIGVFMATFSASLNNLIGXSRILEAIAKDVVFGPLFNFVLKGTYKENPVTAVISTWFVVELFLMMGSLNNIAQLCSVLFLLSYGTINLTCLFLHLASAPNFRPTYKYFSTYTCLIGLLGSIIMMFAVSAIYSAVGILLCLSLILLLNLFSPIRHTNWGSISQALIFHQVRKYLLLLDQRKSHVKFWRPQMLLLVSNPRRNCSLIEFGNAL-KKSGLYVLGHVHVDNLEEHDKDPTLNEIPHWLKLIDFLKVKAFVEVTIARSLRLGVDQLVRISGIGAMKPNTVLLGFKD 572
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000007778 (supercontig:LSalAtl2s:LSalAtl2s455:327857:377250:-1 gene:EMLSAG00000007778 transcript:EMLSAT00000007778 description:"maker-LSalAtl2s455-augustus-gene-2.20") HSP 1 Score: 186.037 bits (471), Expect = 1.310e-47 Identity = 119/432 (27.55%), Postives = 208/432 (48.15%), Query Frame = 0
Query: 410 TSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWG--PLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVL----SGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLD--FKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYY 833
TS + +F + +FFP+ TGI+AG+N SGDL P IP GT++AI+TT Y+ L+ A V+ + D F +++G L + AW ++ G +T+ + + SL GAPR+LQA+AKD + P + F + A +PVR + +I + +LIG++++++ LLS FF+ Y +N + ++ +P WRP FKYY+ +SLLG +LCI VMF+ + A++ ++Y +I +R + WG + + A ++ L H KN+RP+I++L S V L A L L + + + + + A K + + + +K F + + + +G + GLG + PN V++G+ W++ D+ + +
Sbjct: 442 TSDIYTSFVILVGIFFPSCTGIMAGSNRSGDLAXPQKXIPIGTIMAILTTSTVYLSSVLLFA--------ATVDPLLL-RDKFGESIGGSLVVGNI------------------------AWPHPKVVEIGSLLSTIGAGLQSLTGAPRLLQAIAKDGIIPFLAPF-QVQSAKGEPVRALGITLLICEVAVLIGNVDEIAPLLSMFFLMCYGFVNLAXALQTLLRTPNWRPRFKYYHWSLSLLGVVLCIAVMFMSSWYYALLAMGIAGIIYKYIEYRGAEKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILAKLDSQLKVKHKRLFALASQLKAGKGLTIGVTVLDGHFGKMYGEAQAAKFSIQQVIKDEAVKGFADVVVSKDISDGVCHLTQTTGLGGMKPNTVILGWPNSWRKKEEDSWKVF 839 HSP 2 Score: 162.54 bits (410), Expect = 3.122e-40 Identity = 116/424 (27.36%), Postives = 192/424 (45.28%), Query Frame = 0
Query: 1421 AVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWG--PLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKED----VEPRIMDN-----------MKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLS 1827
+FFP+ TG++AG+N SGDL P IP GT+ AILTT Y+ L+ AA+ + LL D+ +G ++ + W ++ G +T+ + L SL G PR+ QAIAKD + P++ F + EP+R + I +LIG+++ ++ LLS FF+ Y +N + + +P WRP FKY++ +SLLG LCI VM + AL + ++Y ++ R + WG + + A SL L H KN+RP+++++ S L K L L +K + ++D K + ++D +K F + + GV + +GLG + N +++G+ W K E+ ++V V S
Sbjct: 454 GIFFPSCTGIMAGSNRSGDLAXPQKXIPIGTIMAILTTSTVYLSSVLLFAATVDPL-------LLRDK---------------FGESIGGSLVVGNIAWPHPKVVEIGSLLSTIGAGLQSLTGAPRLLQAIAKDGIIPFLAPFQVQSAKG-EPVRALGITLLICEVAVLIGNVDEIAPLLSMFFLMCYGFVNLAXALQTLLRTPNWRPRFKYYHWSLSLLGVVLCIAVMFMSSWYYALLAMGIAGIIYKYIEYRGAEKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILAKLDS---------QLKVKHKRLFALASQLKAGKGLTIGVTVLDGHFGKMYGEAQAAKFSIQQVIKDEAVKGFADVVVSKDISDGVCHLTQTTGLGGMKPNTVILGWPNSW---RKKEEDSWKVFVDS 842 HSP 3 Score: 139.428 bits (350), Expect = 3.895e-33 Identity = 84/202 (41.58%), Postives = 119/202 (58.91%), Query Frame = 0
Query: 1178 SNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFS-SQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV 1378
+N S T D K A G + GVY+PC+ NI+G +LF+R+TW+VG AG + F ++ + VT +TAISM+ + TNG + GGG FMISR+LGPEFGGAVG+ F A +A +MYIIG E L+ I + S F + + + N+ R+ G+ L+ + I FIG+ VNK + L +L S V +G FV
Sbjct: 124 ANYSATIQSXPTDPDAKPVQSANLGTLMGVYLPCIQNIFGXILFIRMTWIVGTAGAVEGFFVVFMGCCVTLLTAISMAXIATNGVVPGGGSXFMISRALGPEFGGAVGIXFYXAXXVAAAMYIIGAIEILIIYIVGPAMSLFGPDITNDFVKFNNYRVYGTFLLITMGTIVFIGVRFVNKFAAVALACVLLSIVSIYVGIFV 325 HSP 4 Score: 119.398 bits (298), Expect = 5.810e-27 Identity = 67/185 (36.22%), Postives = 110/185 (59.46%), Query Frame = 0
Query: 193 DSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNG--IVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIG 375
D+ ++ G GVY+ C+ NI+G +LF+R+TW++G AG +EG ++ + VT++T ISM+ ++TNG + GG +MISR+LGPEFGGA+GI F A +A +MYIIG E L+ + G I + + N+ R+ G+ L+ + + +G+ +V + L +L+S V +G
Sbjct: 138 DAKPVQSANLGTLMGVYLPCIQNIFGXILFIRMTWIVGTAGAVEGFFVVFMGCCVTLLTAISMAXIATNGVVPGGGSXFMISRALGPEFGGAVGIXFYXAXXVAAAMYIIGAIEILIIYIVGPAMSLFGPDITNDFVKFNNYRVYGTFLLITMGTIVFIGVRFVNKFAAVALACVLLSIVSIYVG 322 HSP 5 Score: 54.6842 bits (130), Expect = 2.949e-7 Identity = 47/205 (22.93%), Postives = 106/205 (51.71%), Query Frame = 0
Query: 961 GTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFS-DVILLQ--DMTKKADKKT-----RDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPK---REQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
G IDI+W+ DGGL MLLP +L + KLRIF ++++ D + +++ L RIE +V+ +Q D+++ ++T R++ + + + G++++ ++ V ++ ++++++ H+++ ++ + ++ + ++ + +P PK E+I ++M +L+ T + LMVRG V+T
Sbjct: 871 GNIDIWWIVHDGGLLMLLPFLLKQHRTWKNCKLRIFSVAQHEDNSIQMKKDLKTFLYHLRIEADVEVVEMQDSDISEYTYERTLLMEQRNEMLKEMRVSDKRKGKMTRRLSLNKGVGEYKVVDDIII-------HTDKVTNEIEVETVIENSRADVPNIEDKIP-PKVKFSEEID-EVHMEFLEVLTEGLERVLMVRGGGREVIT 1066
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709294|sp|P55013.1|S12A2_SQUAC (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; AltName: Full=NKCC; AltName: Full=Na-K-CL symporter) HSP 1 Score: 791.186 bits (2042), Expect = 0.000e+0 Identity = 418/1039 (40.23%), Postives = 660/1039 (63.52%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDEL---LDESVLSD--INGND---AEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQA----YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLL--ESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSL-ADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFE----DGSESKMSVDSGLASPATLTKRKLGRD---GSVLGRFQKKTKS-SMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +DHYR+ + ++ + RP+L EL LD+ D +NG + AE+ V+ ++G +KFGW GV +RC+LNIWGVMLF+RL+W++G AG+ L +I + +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGGAIG++F AN++AV+MY++GF E++ D+L ++ N ++ ++ ++D+R+IGS+++V++ +++ GMEW + Q+ LL +LL++ V+F +G+ IP++D+ AKGF Y + N + E +FFSVFA+FFPA TGI+AGAN+SGDL DP +AIPKGTLLAI+ T + Y + V C +R A+GN+ + + TV N+ + C KC+YG++ Q+M ++S +GPLI AG F+ATLSSA+ASLV AP++ QA+ KD ++PG+H FS G+G NN+P+RGY+L F I L ILI +LN ++ ++SNFF+A+YALINFSVFHAS+ SPGWRP+F++YN WISL+G ILC GVMF++++ A++T V ++ LY+++ ++KP VNWGSS+Q+ +++ AL+ LT V+DHVKN+RP+ ++++G P RP+L+ H TK + L+ + K++ ++ WL N +K+F++ + L EG + L+ GLG++ PN ++ GFK+DW+Q+L D E Y + AFD + V V+R+ G +IS + A +E + ++ + +N E D S+ S SPA +K D S +K+ K S+ L + D ++ ++ +QF+ K + GTID++WL+DDGGLT+L+P++LTT+ K+ K+R+ F+ ++ +D D R M LLSKFRI+FSD+ +L DM K K F+ MI + ++ + + EA +K E +T+ EL+ + +T Q+R+ E++R+NS A+L+VM+LP+ ++ + LYMAW++ ++ +PP L+VRGN SVLT
Sbjct: 189 TIDAVPRIDHYRH--TVAQLGEKLIRPSLAELHDELDKEPFEDGYVNGEESSPAEEAVSKHVADNKG-VVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGHAGIGLALLVIGTATVVTTITGLSTSAITTNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVRDLLVEH----NALM--IDEMSDIRIIGSVTIVVLFGISVAGMEWEAKAQIVLLGILLLAIVNFTVGTFIPANDKR-AKGFFNYRGEIFSENFVPDFRDGE------DFFSVFAIFFPAATGILAGANISGDLADPQLAIPKGTLLAILITTIVYAGAAVSVGSCIVREATGNLTDA-----IIPGTVTNCTNVACKLGFNFSSCATNKCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHVFSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFRFYNMWISLIGAILCCGVMFVINWWAALLTNVIVLALYIYVTYKKPDVNWGSSTQALTYLNALQHAIRLTGVEDHVKNFRPQCLLMTGAPTSRPALLHLVHAFTKNVGLVVCGHVHTGPRRQALKEISTDQAKYQRWLIKNKMKAFYAPVYAEDLREGTQFLLQAVGLGRMRPNTLVFGFKKDWRQALMKDVENYINAIHDAFDYQYGVVVIRLKEGFNISHLQAQEE----LCTSQEKSAHPKDIVVNLEHSDADSSKPSSKSVSETNSPAVCQDQKDEEDDGKASTQPLLKKEVKDPSVPLNMTD-------QKLLQASSQFQKKQGK-GTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRV-FIGGKINRIDHDRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMI-----EPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLIVMSLPVARKGAVSSALYMAWIETLSKDLPPILLVRGNHQSVLT 1188 HSP 2 Score: 711.449 bits (1835), Expect = 0.000e+0 Identity = 405/1003 (40.38%), Postives = 603/1003 (60.12%), Query Frame = 0
Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDR----------EDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRI-----MDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERW-WLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHA---------LSLDEFQPKSVQLSSKNVDSFGTSSTRN--NSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLS--NRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS-------GDEQGVQRPI-------VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RP+L EL +S A+ V + G +FGWI+GV V C+LNIWGVMLF+RL+W+VG AG+ + +I A +VT++T +S SA+ TNG ++GGG Y++ISRSLGPEFGGA+GL+F A ++AV+MY++GF E + D++ + ++ ++ D +D+RI+GS T+V+L GIS GM K Q++LL +LL + V+F +GTF+P +D KRA G + ++ N PDF+ DGE F VFA+FFPA TG++AGAN+SGDL DP AIPKGTL AIL T Y A+ + + +R A+GN + ++ + F +C+ C+YG + + Q++ L S +GPLI AG F+ATLSSALASLV P++FQA+ KD ++P + F G+G++ EPLRGY+L F I L ILI +LN ++ ++SNFF+A+YA+IN+SVFHAS SPGWRP+F+++N WISL+G LC VM +++ AL T + ++ LYI+V + PDVNWGSS+Q+ +++ AL+ L VEDHVKN+RP+ +++TG P+ RPAL + TK L+ HV PR + +A WL N +KAFYA + G + ++ GLG++ N L+ GF++ W K E Y + +FD V V+R++ G +IS + A E++ T SQ A +D L H+ S+ E +V K+ + G +ST+ EV N D++++ +QF+ K K GTIDV+WL+DDGGLTLL+PY+L+T+KK+ CK+RVF NR DH + MA+LL KFRI+F D+ +L D+ P K+ + F+ M+ EQ + +++ L + +T +R+ E+ +ENS + L+VM+LP+ R+ + LY+AW++ +S+ +PP L VRGN +SVLTFYS
Sbjct: 212 RPSLAELHDELDKEPFEDGYVNGEESSPAEEAVSKHVADNKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGHAGIGLALLVIGTATVVTTITGLSTSAITTNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVRDLLVEHNA----LMID---EMSDIRIIGSVTIVVLFGISVAGMEWEAKAQIVLLGILLLAIVNFTVGTFIPAND-KRAKGFFNYRGEIFSENFVPDFR---DGEDF--FSVFAIFFPAATGILAGANISGDLADPQLAIPKGTLLAILITTIVYAGAAVSVGSCIVREATGNLTDAIIPGTVTNCTNVACKLGFNFSSCATNKCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHVFSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFRFYNMWISLIGAILCCGVMFVINWWAALLTNVIVLALYIYVTYKKPDVNWGSSTQALTYLNALQHAIRLTGVEDHVKNFRPQCLLMTGAPTSRPALLHLVHAFTKNVGLVVCGHV---HTGPRRQALKEISTDQAKYQRWLIKNKMKAFYAPVYAEDLREGTQFLLQAVGLGRMRPNTLVFGFKKDWRQALMKDVENYINAIHDAFDYQYGVVVIRLKEGFNISHLQ-AQEELCT--SQEKSAHPKDIVVNLEHSDADSSKPSSKSVSETNSPAVCQDQKDEEDDGKASTQPLLKKEVKDPSVPLNMTDQKLLQASSQFQKKQGK-GTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDH---DRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMIEPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLIVMSLPVARKGAVSSALYMAWIETLSKDLPPILLVRGNHQSVLTFYS 1191
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|2500792|sp|Q25479.1|NKCL_MANSE (RecName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter; AltName: Full=Na-K-CL symporter) HSP 1 Score: 788.875 bits (2036), Expect = 0.000e+0 Identity = 423/1038 (40.75%), Postives = 656/1038 (63.20%), Query Frame = 0
Query: 138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKA--IKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLF--NDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLA-DAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFA--SDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSV-----DSGLASPATLTKRK---LGRDGSVLGRFQKKTKSSMSLAIL--DKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKF---DALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
LT+E LP +++YRN K RP+L EL + ++++ + EK N + S A IK GW GV++ CLLNIWGVMLFLRL+WV+ QAG+ L II +S IV VITT+SMSA+ TNG++K GGIYY+ISRSLGPEFG ++GI+F AN++A SM IGFC+SL D+LR NG+ + + INDVR++G+++L+++ + +GM+W ++ Q L+ +++ + VDF++G+++ P + EIAKGFVG +A N + + E + NFFSVFA+FFP+VTGI AGAN+SGDLKDPA AIPKGTLLA++ + V+Y + L ++R ASGN+ TDL N TV + +++ C YG+ S +M+++SAWGP IY GC+AATLS+A+ +L+ PR++QA+ D+++PG+ FS+ +G + +P RGY+L F +SL+ +LI DLN ++ L+SNF++A+YALINF FH ++ GWRP+F+YYN W+SL G ++C+ +M L+ + +++T LY+ +++R+P VNWGSS+Q+Q + TAL + AL R +HVKNY P+++VL+G P RP+L+D +L++K SL+ I++ L +K A + +EWL +++F S S GARA ++ G+G+L+PN++LMG+K DW A D Y+ L TAF+ RLAV ++R+ GGLD S + + ++E A ++ EL + ++S + + L++ TLT + + + +++ + M I+ G +++ + +++ F+ K SGT+D++WLYDDGGLT+LLP+I++ R+ +A KLRIF L+ + E++ + RNM LL+KFRI++S + ++QD+T P P++ +L E + KF A + +++ ELQ + +TN QLR+ E++ NS +A LVVM+LP+P++ I PLYMAWL+ +R +PP L VRGN SVLT
Sbjct: 67 LTREALPRMENYRNSKRALK------RPSLGELHGDHLITE----EDEKDQNHRDTKSPTPAVGIKLGWIQGVFIPCLLNIWGVMLFLRLSWVVSQAGIGLSLVIIAISAIVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFGASVGIIFAFANAVAASMNTIGFCDSLNDLLRS-----NGLKITEDPINDVRIVGTVALLVMCIICAIGMDWESKAQNFLIAIIVGAMVDFVVGTIMGPKDNSEIAKGFVGLSSATFVENFKSDFRFSEK--LDQNFFSVFAIFFPSVTGIQAGANISGDLKDPASAIPKGTLLALLISMVSYTLMVLFAGGGALRDASGNI------TDLLIVNGTVTDYSSVSLC---ALNNTCEYGLHNSYSVMQLMSAWGPFIYGGCWAATLSTALTNLLSVPRLIQALGVDRIYPGLIFFSKPYGRHGEPYRGYVLTFFVSLLFLLIADLNTIAPLISNFYLASYALINFCTFHRALVRPLGWRPTFRYYNMWLSLAGFLMCVAIMLLVHWVMSLVTFAIFFTLYLIVHYRRPDVNWGSSTQAQMYKTALSSAHALARTGEHVKNYWPQLLVLAGRPQARPALVDLGNLISKAGSLMIVGDISQEKLSYKVRSARARSDDEWLRGRKVRAFCSRVHGFSFEPGARALVQGSGVGRLAPNVLLMGYKSDWTTCPANDLVSYFNVLHTAFENRLAVAIVRVSGGLDYSAVVSEGAEEGAAGSLTATSSSGELRVRRDGLIMHADSDLDIRDTQPKHNLSNLLTLTTSRSFTISECKEKDKKKKERKPNDMHRQIVYNTASGLELSKFQLAQMSLFQKKQ-ESGTLDVWWLYDDGGLTILLPYIISQRSAWANCKLRIFALANRLHEMELEERNMANLLAKFRIDYSSLTMVQDITDP---------------PQPET-----KALFDETIKKFTEESASPDCRISDMELQTLAVKTNRQLRLRELLLANSKDARLVVMSLPMPRKGSISAPLYMAWLEMMSRDLPPMLFVRGNHTSVLT 1057 HSP 2 Score: 722.62 bits (1864), Expect = 0.000e+0 Identity = 399/964 (41.39%), Postives = 580/964 (60.17%), Query Frame = 0
Query: 1189 KDTVKKDTG---PA---RFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKR-AMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCS----NRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDI--SGAHEKVMTA--ASQASGASDEDRFSMLNHA---LSLDEFQPK---SVQLSSKNVDSFGTSSTRN-------------NSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSG--DEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
KD +DT PA + GWIQGV++PCLLNIWGVMLFLRL+WVV QAG+ S II I+ +V +T +SMSA+CTNG++KGGGIY++ISRSLGPEFG +VG++F A ++A SM IGFC++L D++ + +E NDVRI+G+ L+++ I IGM +K Q L+ +++ + VDF++GT + P D A G VG N DF+ + +Q F VFA+FFP+VTG+ AGAN+SGDLKDP SAIPKGTL A+L + SY + L +R ASGN +LL+ + + S N C YG + ++QL S WGP IY GC+AATLS+AL +L+ PR+ QA+ D+++P + F + +GR EP RGY+L F ++L +LI DLN ++ L+SNF++A+YA+IN+ FH + GWRP+F+Y+N W+SL G +C+ +M+L+ +L T LY+ V R PDVNWGSS+Q+Q + TAL S L +HVKNY P+L+V+ G P RPAL D NLI+K SL+ + + +E + ++ WLR ++AF + SF G R V+ SG+G+L+ N+L++G++ W P Y+ VL +F+ L+V ++RV GGLD S + GA E + A+ +SG R ++ HA L + + QPK S L+ SF S + + ++ Y G + + + +M+ F+ K Q+ GT+DV+WLYDDGGLT+LLPYI+S R +A CK+R+F L+NR + E MA+LL KFRI++ + ++ D+T P+ ET A F + +E +S++ L +T LR+ E+ NS + LVVM+LP+PR+ I LY+AWL+ +SR +PP L+VRGN SVLTFYS
Sbjct: 105 KDQNHRDTKSPTPAVGIKLGWIQGVFIPCLLNIWGVMLFLRLSWVVSQAGIGLSLVIIAISAIVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFGASVGIIFAFANAVAASMNTIGFCDSLNDLLRSNGLKI------TEDPINDVRIVGTVALLVMCIICAIGMDWESKAQNFLIAIIVGAMVDFVVGTIMGPKDNSEIAKGFVGLSSATFVENFKSDFRFSEKLDQ-NFFSVFAIFFPSVTGIQAGANISGDLKDPASAIPKGTLLALLISMVSYTLMVLFAGGGALRDASGNITDLLIVNGTVTDYSSVSLCALNNTCEYGLHNSYSVMQLMSAWGPFIYGGCWAATLSTALTNLLSVPRLIQALGVDRIYPGLIFFSKPYGRHGEPYRGYVLTFFVSLLFLLIADLNTIAPLISNFYLASYALINFCTFHRALVRPLGWRPTFRYYNMWLSLAGFLMCVAIMLLVHWVMSLVTFAIFFTLYLIVHYRRPDVNWGSSTQAQMYKTALSSAHALARTGEHVKNYWPQLLVLAGRPQARPALVDLGNLISKAGSLMIVGDISQEKLSYKVRSARARSDDEWLRGRKVRAFCSRVHGFSFEPGARALVQGSGVGRLAPNVLLMGYKSDWTTCPANDLVSYFNVLHTAFENRLAVAIVRVSGGLDYSAVVSEGAEEGAAGSLTATSSSGELRVRRDGLIMHADSDLDIRDTQPKHNLSNLLTLTTSRSFTISECKEKDKKKKERKPNDMHRQIVYNTASGLELSKFQLAQMSLFQ-KKQESGTLDVWWLYDDGGLTILLPYIISQRSAWANCKLRIFALANRLHEMELEERNMANLLAKFRIDYSSLTMVQDITDPPQPETKALFDETIKKFTEESASPDCRISDMELQTLAVKTNRQLRLRELLLANSKDARLVVMSLPMPRKGSISAPLYMAWLEMMSRDLPPMLFVRGNHTSVLTFYS 1060
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|341941994|sp|P55012.2|S12A2_MOUSE (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1) HSP 1 Score: 783.097 bits (2021), Expect = 0.000e+0 Identity = 417/1034 (40.33%), Postives = 666/1034 (64.41%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSS-----------EGKAI-KFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEI-LNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLL--ESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDW-QQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLN--FEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +DHYR+ + ++ + RP+L EL DE + E G E+S+ E K + KFGW GV +RC+LNIWGVMLF+RL+W++GQAG+ + +I ++ +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGGAIG++F AN++AV+MY++GF E+++++L+++ + ++ IND+R+IG+I++V++L +++ GMEW + Q+ LL++LL++ DF+IG+ I S + + KGF GY + + N + ++ET FFSVFA+FFPA TGI+AGAN+SGDL DP AIPKGTLLAI+ T V Y+ + V C +R A+GNV + T+L N T LN +Y CE C+YG++ + Q+M ++S + PLI AG F+ATLSSA+ASLV AP++ QA+ KD ++P F++G+G NN+P+RGYIL F+I+L ILI +LN ++ ++SNFF+A+YALINFSVFHAS+ SPGWRP+FKYYN WISL+G ILC VMF++++ A++T V ++ LY+++ ++KP VNWGSS+Q+ ++++AL+ L+ V+DHVKN+RP+ +V++G+P RP+L+ H TK + L+ + + K++ ++ WL N +K+F++ D L EGA+ ++ GLG++ PN +++GFK+DW Q + D + Y AFD++ V V+R+ GLDIS + +E + + + + +N + S+ SG S +T++ DG + K +S + L+ +++ +E TQF+ K ++ TID++WL+DDGGLT+L+P++LTT+ K+ K+R+ F+ ++ +D D R M LLSKFRI+FSD+++L D+ K K+ F MI P+ ++ + + + DK E +T+ EL+ + +T Q+R+ E+++++SS A+++VM+LP+ ++ + LYMAWL+ ++ +PP L+VRGN SVLT
Sbjct: 210 TMDAVPRIDHYRH--TAAQLGEKLLRPSLAELHDE-----LEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEH------SILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFI-SLESKKPKGFFGYKSEIFNENFGPDFREEET------FFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDT-ITTELTNCTSAACKLNFDFSY--CESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEE----LLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKS---ITQKDEEEDGKTPTQPLLKKESKGPIVPLNV----ADQKLLEASTQFQKKQGKN-TIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRV-FIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIE-PY----RLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLT 1202 HSP 2 Score: 725.702 bits (1872), Expect = 0.000e+0 Identity = 401/956 (41.95%), Postives = 602/956 (62.97%), Query Frame = 0
Query: 1197 GPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSS-QFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDR---------EDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPR--IMDNM---KAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYP-KFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSV---QLSSKNVDSFGTSSTRN--NSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLS--NRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS-------GDEQGVQRPI-------VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
G +FGWI+GV V C+LNIWGVMLF+RL+W+VGQAG+ S +I +A +VT++T +S SA+ TNG ++GGG Y++ISRSLGPEFGGA+GL+F A ++AV+MY++GF E +++++ + S D I ND+RI+G+ T+V+LLGIS GM K Q++LL++LL + DF+IGTF+ + K+ G G+ ++ N PDF+ E+ F VFA+FFPA TG++AGAN+SGDL DP SAIPKGTL AIL T Y+ A+ + + +R A+GN + + + F C + C+YG + N Q++ + S + PLI AG F+ATLSSALASLV P++FQA+ KD ++P + F +G+G++ EPLRGY+L F IAL ILI +LN ++ ++SNFF+A+YA+IN+SVFHAS SPGWRP+FKY+N WISL+G LC +VM +++ AL T + ++ LYI+V + PDVNWGSS+Q+ ++++AL+ L+ VEDHVKN+RP+ +V+TG+P+ RPAL + TK L+ HV + PR M M +A WL N +KAFYA D G + ++ +GLG++ N L++GF++ W + + Y + +FD+ V V+R++ GLDIS + G E + +SQ +D ++++ D+ KS ++ K+ + G + T+ E I N D++++ TQF+ K K TIDV+WL+DDGGLTLL+PY+L+T+KK+ CK+RVF NR DH + MA+LL KFRI+F D+++L D+ PKKE + F M+ EQ + + +++ L + +T +R+ E+ KE+S + ++VM+LP+ R+ + LY+AWL+ +S+ +PP L VRGN +SVLTFYS
Sbjct: 274 GVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEI--------NDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFIS-LESKKPKGFFGYKSEIFNENFGPDFR-----EEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHV---HMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELL---SSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKDEEEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGK-NTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDH---DRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLTFYS 1205
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709292|sp|P55011.1|S12A2_HUMAN (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1) HSP 1 Score: 782.326 bits (2019), Expect = 0.000e+0 Identity = 414/1039 (39.85%), Postives = 663/1039 (63.81%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGK------------AIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDT-VGEILNITQA-------YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLL--ESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDW-QQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +DHYR+ + ++ + RP+L EL DE + E G E+S+ + +KFGW GV +RC+LNIWGVMLF+RL+W++GQAG+ + +I ++ +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGGAIG++F AN++AV+MY++GF E+++++L+++ + ++ IND+R+IG+I++V++L +++ GMEW + Q+ LL++LL++ DF+IG+ IP ++ KGF GY + + N + ++ET FFSVFA+FFPA TGI+AGAN+SGDL DP AIPKGTLLAI+ T + Y+ + V C +R A+GNV NDT V E+ N T A + CE C+YG++ + Q+M ++S + PLI AG F+ATLSSA+ASLV AP++ QA+ KD ++P F++G+G NN+P+RGYIL F+I+L ILI +LN ++ ++SNFF+A+YALINFSVFHAS+ SPGWRP+FKYYN WISLLG ILC VMF++++ A++T V ++ LY+++ ++KP VNWGSS+Q+ +++ AL+ L+ V+DHVKN+RP+ +V++G P RP+L+ H TK + L+ + + K++ ++ WL N +K+F++ D L EGA+ ++ GLG++ PN +++GFK+DW Q + D + Y AFD++ V V+R+ GLDIS + +E + + + + ++ E +S + L S +T + DG + K +S + L+ +++ +E TQF+ K ++ TID++WL+DDGGLT+L+P++LTT+ K+ K+R+ F+ ++ +D D R M LLSKFRI+FSD+++L D+ K K+ F+ +I P+ ++ + + + DK E +T+ EL+ + +T Q+R+ E+++++SS A+++VM+LP+ ++ + LYMAWL+ ++ +PP L+VRGN SVLT
Sbjct: 217 TMDAVPRIDHYRH--TAAQLGEKLLRPSLAELHDE-----LEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEH------SILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKK-PKGFFGYKSEIFNENFGPDFREEET------FFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNV----------NDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEE----LLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPL-SEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNV----ADQKLLEASTQFQKKQGKN-TIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRV-FIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIE-PY----RLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLT 1209 HSP 2 Score: 728.013 bits (1878), Expect = 0.000e+0 Identity = 402/954 (42.14%), Postives = 606/954 (63.52%), Query Frame = 0
Query: 1197 GPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSS-QFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDR---------EDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPR--IMDNM---KAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYP-KFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQ-LSSKNVDSFGTSSTRN--NSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLS--NRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS-------GDEQGVQRPI-------VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
G +FGWI+GV V C+LNIWGVMLF+RL+W+VGQAG+ S +I +A +VT++T +S SA+ TNG ++GGG Y++ISRSLGPEFGGA+GL+F A ++AV+MY++GF E +++++ + S D I ND+RI+G+ T+V+LLGIS GM K Q++LL++LL + DF+IGTF+P + K+ G G+ ++ N PDF+ E+ F VFA+FFPA TG++AGAN+SGDL DP SAIPKGTL AIL T Y+ A+ + + +R A+GN + ++ + F +C + C+YG + N Q++ + S + PLI AG F+ATLSSALASLV P++FQA+ KD ++P + F +G+G++ EPLRGY+L F IAL ILI +LN ++ ++SNFF+A+YA+IN+SVFHAS SPGWRP+FKY+N WISLLG LC +VM +++ AL T + ++ LYI+V + PDVNWGSS+Q+ +++ AL+ L+ VEDHVKN+RP+ +V+TG P+ RPAL + TK L+ HV + PR M M +A WL N +KAFYA D G + ++ +GLG++ N L++GF++ W + + Y + +FD+ V V+R++ GLDIS + G E+++++ ++ G D + LD +P S + ++ K + G ++T+ E I N D++++ TQF+ K K TIDV+WL+DDGGLTLL+PY+L+T+KK+ CK+RVF NR DH + MA+LL KFRI+F D+++L D+ PKKE + F+ ++ EQ + + +++ L + +T +R+ E+ KE+S + ++VM+LP+ R+ + LY+AWL+ +S+ +PP L VRGN +SVLTFYS
Sbjct: 281 GVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEI--------NDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIP-LESKKPKGFFGYKSEIFNENFGPDFR-----EEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHV---HMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQG-QEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGK-NTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDH---DRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS 1212
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709297|sp|P55016.1|S12A1_RAT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 776.933 bits (2005), Expect = 0.000e+0 Identity = 411/1050 (39.14%), Postives = 662/1050 (63.05%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLS-DINGNDAEKGVNG----AEDSSEGK------AIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDS--VERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQA--------YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR--LSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQS-LADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGR----------PVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +++YRN S S P+ +RP+L E+ ++ + + A++ NG ++ +E K +KFGW GV +RC+LNIWGVMLF+RL+W++G+AG+ G+ II LS +VT +T ISMSA+ TNG ++ GG YY+ISRSLGPEFGG+IG++F AN++ V+MY++GF E+++D+L++ DS V+ ND+R+IGSI++V++L +++ GMEW + Q+ LL++LL+ +F IG+VIPS++E+ ++GF Y ++ N ++T+ E FFSVFA+FFPA TGI+AGAN+SGDL+DP AIP+GT+LAI T V Y+ + V C +R A+G++ NDTV +N + + C+ C YG++ + Q+M ++S +GPLI AG F+ATLSSA+ASLV AP+V QA+ KD +F G+ F++G+G NN+P+RGY L F+I++ ILI +LN ++ ++SNFF+A+YALINFS FHAS SPGWRP++ YN W+SL G ILC VMF++++ A+IT V + LY+++ ++KP VNWGSS+Q+ S+++AL LT V+DHVKN+RP+ +VL+G P+ RP+L+D H TK L + + + L K++++ + WL N IK+F++ D +G R+ L+ GLG++ PN +++G+K++W+++ L++ E Y + AFD + V ++RI G DIS + ++ + + + R E + + + E+G G + G F+K K +++ KD N++ VE QF+ K + GTID++WL+DDGGLT+L+P+ILT R K+ KLRI ++ ++ ++E+ +M +LLSKFRI+F+D+ ++ D+ K +K++ F+ MI P+ ++ +S +K +T+ EL+ E++ Q+R+ E+++++S A+L+V++LP+ ++ I LYMAWL+ T+ +PP L+VRGN +VLT
Sbjct: 101 TMDAVPKIEYYRNTGSVSG--PKVNRPSLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFRFANAVRVAMYVVGFAETVVDLLKE--------SDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIGIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTEGE------GFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVRACVVRDATGSM----------NDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKL-EQERLALEAAIKDNDCEEGK------------------------GGIRGLFKKAGKLNITKPAPKKDSNISTIQSMHVGEFNQKLVEASAQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIE-PY----RLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLT 1092 HSP 2 Score: 716.842 bits (1849), Expect = 0.000e+0 Identity = 397/1005 (39.50%), Postives = 612/1005 (60.90%), Query Frame = 0
Query: 1155 SRPTLDELLCR-SRNGSIYPCEGRSNQSG---TADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDRED---------ELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRI-----MDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGA--------HEKVMTAASQASGASDEDR---FSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV-------------SGDEQGVQRPI-VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+RP+L E+ + ++N ++ P +G D QA++ + TG +FGW++GV V C+LNIWGVMLF+RL+W+VG+AG+ II ++ +VT++T ISMSA+CTNG ++GGG Y++ISRSLGPEFGG++GL+F A ++ V+MY++GF E ++D++ + D ++ D ND+RI+GS T+V+LLGIS GM K Q++LL++LL +F IGT +P ++EK++ G + + N P F +GE F VFA+FFPA TG++AGAN+SGDL+DP AIP+GT+ AI T +Y+ A+ + A +R A+G+ + ++ + F C + C YG + N Q++ + S +GPLI AG F+ATLSSALASLV P+VFQA+ KD +F ++ F +G+G++ EPLRGY L F IA+ ILI +LN ++ ++SNFF+A+YA+IN+S FHAS+ SPGWRP++ +N W+SL G LC VM +++ A+ T + + LYI+V + PDVNWGSS+Q+ S+++AL + L VEDHVKN+RP+ IV+TG P RPAL D + TK S L V V PR M++ A WL N IKAFYA D F GVR+ ++ SGLG++ N L+IG+++ W P E Y ++ +FD + V ++R+ G DIS + E++ A+ +E + + A L+ +P + K + T + + E ++++V QF+ K K GTIDV+WL+DDGGLTLL+PYIL+ RKK+ CK+R+ ++ + + + EE MASLL KFRI+F D+ I+ D+ P KE+ F+ M+ + ++ + P +++ L +++ +R+ E+ +E+S + L+V++LP+ R+ I LY+AWL+ +++++PP L VRGN ++VLTFYS
Sbjct: 123 NRPSLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFRFANAVRVAMYVVGFAETVVDLL----KESDSMMVDP---TNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIGIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSF---TEGEGF--FSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVRACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVF---VGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKP-----APKKDSNISTIQSMHVGE----------FNQKLVEASAQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS 1095
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|27151793|sp|P55014.2|S12A1_MOUSE (RecName: Full=Solute carrier family 12 member 1; AltName: Full=BSC1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 774.622 bits (1999), Expect = 0.000e+0 Identity = 413/1050 (39.33%), Postives = 664/1050 (63.24%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLS-DINGNDAEKGVNG----AEDSSEGK------AIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDS--VERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQA--------YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR--LSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQS-LADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGR----------PVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +++YRN S S P+ +RP+L E+ ++ + + A++ NG ++ +E K +KFGW GV +RC+LNIWGVMLF+RL+W++G+AG+ G+ II LS +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGG+IG++F AN++AV+MY++GF E+++D+L++ DS V+ ND+R+IGSI++V++L +++ GMEW + Q+ LL++LL++ +F IG+VIPS++E+ ++GF Y ++ N ++TK E FFSVFA+FFPA TGI+AGAN+SGDL+DP AIP+GT+LAI T V Y+ + VA C +R A+G++ NDT+ +N + + C+ C YG++ + Q+M ++S +GPLI AG F+ATLSSA+ASLV AP+V QA+ KD +F G+ F++G+G NN+P+RGY L F+I++ ILI +LN ++ ++SNFF+A+YALINFS FHAS SPGWRP++ YN W+SL G ILC VMF++++ A+IT V + LY+++ ++KP VNWGSS+Q+ S+++AL LT V+DHVKN+RP+ +VL+G P+ RP+L+D H TK L + + + L K++++ + WL N IK+F++ D +G R+ L+ GLG++ PN +++G+K++W+++ L++ E Y + AFD + V ++RI G DIS + ++ + + + R E + + E+G G + G F+K K +++ KDG N++ VE QF+ K + GTID++WL+DDGGLT+L+P+ILT R K+ KLRI ++ + ++E+ +M +LLSKFRI+F+D+ ++ D+ K +K++ F+ MI P+ ++ +S +K +T+ EL+ E++ Q+R+ E+++++S A+L+V++LP+ ++ I LYMAWL+ T+ +PP L+VRGN +VLT
Sbjct: 101 TMDAVPKIEYYRNTGSVSG--PKVNRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKE--------SDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGE------GFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSM----------NDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKL-EQERLALEAAIKDNECEEGK------------------------GGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIE-PY----RLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLT 1092 HSP 2 Score: 711.835 bits (1836), Expect = 0.000e+0 Identity = 397/1004 (39.54%), Postives = 613/1004 (61.06%), Query Frame = 0
Query: 1155 SRPTLDELLCR-SRNGSIYPCEGRSNQSG---TADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDRED---------ELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRI-----MDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGN----------PIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV-------------SGDEQGVQRPI-VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+RP+L E+ + ++N ++ P +G D QA++ + TG +FGW++GV V C+LNIWGVMLF+RL+W+VG+AG+ II ++ +VTS+T +S SA+ TNG ++GGG Y++ISRSLGPEFGG++GL+F A ++AV+MY++GF E ++D++ + D ++ D ND+RI+GS T+V+LLGIS GM K Q++LL++LL + +F IGT +P ++EK++ G + + N P F K GE F VFA+FFPA TG++AGAN+SGDL+DP AIP+GT+ AI T +Y+ A+ +AA +R A+G+ + ++ + F C + C YG + N Q++ + S +GPLI AG F+ATLSSALASLV P+VFQA+ KD +F ++ F +G+G++ EPLRGY L F IA+ ILI +LN ++ ++SNFF+A+YA+IN+S FHAS+ SPGWRP++ +N W+SL G LC VM +++ A+ T + + LYI+V + PDVNWGSS+Q+ S+++AL + L VEDHVKN+RP+ IV+TG P RPAL D + TK S L V V PR M++ A WL N IKAFYA D F GVR+ ++ SGLG++ N L+IG+++ W P E Y ++ +FD + V ++R+ G DIS + +++ +++R + L A+ +E + + + + N +DGN ++++V QF+ K K GTIDV+WL+DDGGLTLL+PYIL+ RKK+ CK+R+ ++ + + EE MASLL KFRI+F D+ I+ D+ P KE+ F+ M+ + ++ + P +++ L +++ +R+ E+ +E+S + L+V++LP+ R+ I LY+AWL+ +++++PP L VRGN ++VLTFYS
Sbjct: 123 NRPSLLEIHEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLL----KESDSMMVDP---TNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTK---GEGF--FSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVF---VGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKL--------EQERLA-LEAAIKDNE-----CEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGEFNQKLVEASAQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS 1095
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|212276464|sp|Q13621.2|S12A1_HUMAN (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 770.385 bits (1988), Expect = 0.000e+0 Identity = 428/1104 (38.77%), Postives = 677/1104 (61.32%), Query Frame = 0
Query: 85 PSPYLPTRNGN--RKRSSVTIAPGTILEKPLPSQRKMTSKQRSSFILTPKPLKHYL-------TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDE---------SVLSDINGNDAEKGVNGAEDSSEGKA--IKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDS--VERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQA--------YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR--LSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQS-LADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMF-ASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
P Y T G+ +KR ++ PG Q T+K +SF Y T + +P +++YRN S S P+ +RP+L E+ ++ S + D G AE+ + +A +KFGW GV +RC+LNIWGVMLF+RL+W++G+AG+ G+ II LS +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGG+IG++F AN++AV+MY++GF E+++D+L++ DS V+ ND+R+IGSI++V++L +++ GMEW + Q+ LL++LL++ +F IG+VIPS++E+ ++GF Y ++ N +TK E FFSVFA+FFPA TGI+AGAN+SGDL+DP AIP+GT+LAI T V Y+ + V C +R A+GN+ NDT+ +N + + C C YG++ + Q+M ++S +GPLI AG F+ATLSSA+ASLV AP+V QA+ KD ++ + F++G+G NN+P+RGYIL F+I++ ILI +LN ++ ++SNFF+A+YALINFS FHAS SPGWRP++ YN W+SL G +LC VMF++++ A+IT V LYV++ +KP VNWGSS+Q+ S+++AL LT V+DHVKN+RP+ +VL+G P+ RP+L+D H TK L + + + L K++++ + WL N IK+F++ D +G R+ L+ GLG++ PN +++G+K++W+++ L + E Y + AFD + V ++RI G DIS++ +E ++ R + + + E G + +G +TK +DGS+ T SM + N++ VE TQF+ K GTID++WL+DDGGLT+L+P+ILT R K+ KLRI ++ ++ ++E+ M +LLSKFRI+F+D+ ++ D+ + +K++ F+ MI P+ ++ +S +K +T+ EL+ E++ Q+R+ E+++++S A+L+V++LP+ ++ I LYMAWL+ T+ +PP L+VRGN +VLT
Sbjct: 42 PPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISG--PKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKE--------SDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGE------GFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNM----------NDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAG---KLNITKTTPKKDGSI------NTSQSMHVG-------EFNQKLVEASTQFKKKQ-EKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIE-PY----RLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLT 1096 HSP 2 Score: 713.761 bits (1841), Expect = 0.000e+0 Identity = 397/1000 (39.70%), Postives = 611/1000 (61.10%), Query Frame = 0
Query: 1155 SRPTLDELLCR-SRNGSIYPCEGRSNQSGTA---DCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDRED---------ELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRI-----MDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDI-----SGAHEKVMTAASQASGASDE-DRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV-------------SGDEQGVQRPI-VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+RP+L E+ + ++N ++ P +G D QA++ G +FGW++GV V C+LNIWGVMLF+RL+W+VG+AG+ II ++ +VTS+T +S SA+ TNG ++GGG Y++ISRSLGPEFGG++GL+F A ++AV+MY++GF E ++D++ + D ++ D ND+RI+GS T+V+LLGIS GM K Q++LL++LL + +F IGT +P ++EK++ G + + N P F K GE F VFA+FFPA TG++AGAN+SGDL+DP AIP+GT+ AI T +Y+ A+ + A +R A+GN + ++ + F C + C YG + N Q++ + S +GPLI AG F+ATLSSALASLV P+VFQA+ KD ++ ++ F +G+G++ EPLRGY+L F IA+ ILI +LN ++ ++SNFF+A+YA+IN+S FHAS+ SPGWRP++ +N W+SL G LC VM +++ A+ T + LY++V + PDVNWGSS+Q+ S+++AL + L VEDHVKN+RP+ IV+TG P RPAL D + TK S L V V PR M++ A WL N IKAFYA D F GVR+ ++ SGLG++ N L+IG+++ W P E Y ++ +FD + V ++R+ G DIS + + A +A+ +E + S L + + + K S TS + + E ++++V TQF+ K +K GTIDV+WL+DDGGLTLL+PYIL+ RKK+ CK+R+ ++ + + + EE MASLL KFRI+F D+ I+ D+ P KE+ F+ M+ + ++ + P +++ L +++ +R+ E+ +E+S + L+V++LP+ R+ I LY+AWL+ +++++PP L VRGN ++VLTFYS
Sbjct: 127 NRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLL----KESDSMMVDP---TNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTK---GEGF--FSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVF---VGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGE----------FNQKLVEASTQFKKKQEK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS 1099
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709296|sp|P55015.1|S12A1_RABIT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter) HSP 1 Score: 762.681 bits (1968), Expect = 0.000e+0 Identity = 408/1051 (38.82%), Postives = 658/1051 (62.61%), Query Frame = 0
Query: 139 TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESV------------LSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDS--VERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQA--------YDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR--LSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQS-LADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGR----------PVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
T + +P +++YRN S S P+ +RP+L E+ ++ +++ G E+ E+ ++ A+KFGW GV +RC+LNIWGVMLF+RL+W++G+AG+ G+ II LS +VT IT +S SA++TNG ++ GG YY+ISRSLGPEFGG+IG++F AN++AV+MY++GF E+++D+L++ DS V+ ND+R+IGSI++V++L +++ GMEW + Q+ LL++LL++ +F IG+VIPS++E+ ++GF Y ++ N ++TK E FFSVFA+FFPA TGI+AGAN+SGDL+DP AIP+GT+LAI T V Y+ + V C +R A+G++ NDT+ +N + + C C YG++ + Q+M ++S +GPLI AG F+ATLSSA+ASLV A +V QA+ KD ++ + F++G+G NN+P+RGYIL F+I++ ILI +LN ++ ++SNFF+A+YALINFS FHAS SPGWRP++ YN W+SL G +LC VMF++++ A+IT V LY+++ ++KP VNWGSS+Q+ S+++AL LT V+DHVKN+RP+ VL+G P+ RP+L+D + TK L + + + L K++++ + WL N IK+F++ D +G R+ L+ GLG++ PN +++G+K+ W+++ L + E Y + AFD + V ++RI G DIS++ E+ + + + R E + + E+G +G + G F+K K +++ KD N++ VE TQF+ K + GTID++WL+DDGGLT+L+P+ILT R K+ KLRI ++ ++ ++E+ M +LLSKFRI+F+D+ ++ D+ K +K++ F+ MI P+ ++ +S +K +T+ EL+ E++ Q+R+ E+++++S A+L+V++LP+ ++ I LYMAWL+ T+ +PP L+VRGN +VLT
Sbjct: 104 TMDAVPKIEYYRNTGSVSG--PKVNRPSLLEIHEQLAKNVAVTPGSADRVANGEGMPGEEHAENKEEDNKAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVVIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKE--------SDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKGE------GFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVGACVVRDATGSM----------NDTIISGINCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAAKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCFVLTGGPMTRPALLDITYAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKKWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELEKL-EQERLALEATIKDNECEEG------------------------NGGIRGLFKKVGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINIKPNKESWKFFEEMIE-PY----RLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLT 1096 HSP 2 Score: 699.893 bits (1805), Expect = 0.000e+0 Identity = 393/1006 (39.07%), Postives = 610/1006 (60.64%), Query Frame = 0
Query: 1155 SRPTLDELLCR-SRNGSIYP--CEGRSNQSGTADCQAKDTVKKDT--GPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDRED---------ELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRI-----MDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDI--------SGAHEKVMTAASQASGASDEDR---FSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV-------------SGDEQGVQRPI-VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+RP+L E+ + ++N ++ P + +N G + + ++D G +FGW++GV V C+LNIWGVMLF+RL+W+VG+AG+ II ++ +VTS+T +S SA+ TNG ++GGG Y++ISRSLGPEFGG++GL+F A ++AV+MY++GF E ++D++ + D ++ D ND+RI+GS T+V+LLGIS GM K Q++LL++LL + +F IGT +P ++EK++ G + + N P F K GE F VFA+FFPA TG++AGAN+SGDL+DP AIP+GT+ AI T +Y+ A+ + A +R A+G+ + ++ + F C + C YG + N Q++ + S +GPLI AG F+ATLSSALASLV +VFQA+ KD ++ ++ F +G+G++ EPLRGY+L F IA+ ILI +LN ++ ++SNFF+A+YA+IN+S FHAS+ SPGWRP++ +N W+SL G LC VM +++ A+ T + LYI+V + PDVNWGSS+Q+ S+++AL + L VEDHVKN+RP+ V+TG P RPAL D TK S L V V PR M++ A WL N IKAFYA D F GVR+ ++ SGLG++ N L+IG++++W P E Y ++ +FD + V ++R+ G DIS + E++ A+ +E + L+ +P + SS N T + + E ++++V TQF+ K K GTIDV+WL+DDGGLTLL+PYIL+ RKK+ CK+R+ ++ + + + EE MASLL KFRI+F D+ ++ D+ P KE+ F+ M+ + ++ + P +++ L +++ +R+ E+ +E+S + L+V++LP+ R+ I LY+AWL+ +++++PP L VRGN ++VLTFYS
Sbjct: 126 NRPSLLEIHEQLAKNVAVTPGSADRVANGEGMPGEEHAENKEEDNKAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVVIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLL----KESDSMMVDP---TNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTK---GEGF--FSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGINCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAAKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCFVLTGGPMTRPALLDITYAFTKNSGLCICCEVF---VGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKKWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELEKLEQERLALEATIKDNECEEGNGGIRGLFKKVGKLNITKPTPKKDSSIN-----TIQSMHVGE----------FNQKLVEASTQFKKKQGK-GTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRI-YVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINIKPNKESWKFFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS 1099
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|313104194|sp|P55017.3|S12A3_HUMAN (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl cotransporter; Short=NCC; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter) HSP 1 Score: 696.041 bits (1795), Expect = 0.000e+0 Identity = 384/1053 (36.47%), Postives = 613/1053 (58.21%), Query Frame = 0
Query: 137 YLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDEL---------------LDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEG---------------RKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLL--ESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSL-ADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEA-VDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKM-PPFLMVRGNQDSVLT 1154
Y T +V+P +HY N S G P + RPTL +L D ++ E + + + + ++FGW GV +RC+LNIWGV+L+LRL W+ QAG++ II LS VT IT +S+SA+STNG++K+GG Y++ISRSLGPE GG+IG++F AN++ V+M+ +GF E++ D+L++Y V+ IND+R+I +S+ ++LA+++ GMEW ++ Q+ L+++VS ++++G++IP S+++ +KGF Y + NL + G FF +F++FFP+ TGI+AGAN+SGDLKDPA+AIPKGTL+AI T ++Y+ + C +R ASG + NDTV T + CEG C+YG++ Q M ++S + PLI AG F ATLSSA+A LV A +V Q + +D+L+P I F +G+G N +PVRGY+L + I++ I+I +LN ++ ++SNFF+ +YALINFS FHASIT+SPGWRPSF+YYNKW +L G I+ + +MFL+ + A+I ++ L +++ ++KP+VNWGSS Q+ S+ AL L V+DH+KNYRP+ +VL+G P RP+L+DF T+ LSL+ + I ++ + + +WLN IK+F+S + L G + ++ GLG++ PN++++GFK++WQ + A E+Y L AFD V V+R+ GL++S+M + A EDG E+ VD P L K + + T F+++ + TIDIYWL+DDGGLT+L+P++L + +++K K+R+ F+ ++ +D++ + + +LLSKFR+ F +V +L D+ + + F+ MI G K EA V++ +++EE+ ++ ++ Q+R+ EIV S +A L+V+TLP+ ++ + P LYMAWL+ ++ + PP +++RGNQ++VLT
Sbjct: 58 YNTIDVVPTYEHYAN--STQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPI------VDPINDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDW-----RGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASG----------VLNDTV------TPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPA---------------EDGKEASARVD-----PKALVKEE------------------------------------QATTIFQSEQGKK-TIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRV-FVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDG------FKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLT 1017 HSP 2 Score: 655.596 bits (1690), Expect = 0.000e+0 Identity = 367/952 (38.55%), Postives = 569/952 (59.77%), Query Frame = 0
Query: 1193 KKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF-DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELL---------LDREDELYFQNCSNRN-CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMD-NMKAFVH-HWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWW-LYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAH-EKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS--------GDEQGVQR-----PI-VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSM-PPFLYVRGNQESVLTFY 2114
K P RFGW++GV + C+LNIWGV+L+LRL W+ QAG++ ++ II ++ VTS+T +S+SA+ TNG++K GG YF+ISRSLGPE GG++GL+F A ++ V+M+ +GF E + D++ ++ + D I ND+RI+ ++ +LL IS GM +K Q+L LV++ S ++L+GT +PPS++K + G + D+ NL PD++ G F +F++FFP+ TG++AGAN+SGDLKDP AIPKGTL AI T SY+ + I + +R ASG + + L F C+ ++ C YG + Q + + S + PLI AG F ATLSSALA LV +VFQ + +D+L+P I FG+G+G++KEP+RGYLL +AIA+ I+I +LN ++ ++SNFF+ +YA+IN+S FHAS NSPGWRPSF+Y+NKW +L G + +V+M L+ AL + ++ L ++V + P+VNWGSS Q+ S+ AL LN VEDH+KNYRP+ +V+TG P+ RPAL DF T+ SL+ HV+ + R+ + + A H WL IKAFY+ + GV+ ++ +GLG++ N+L++GF++ W +P E+Y +L +FD + V V+R++ GL++S + AH V A AS PK++ + E + T Q TID+YWL+DDGGLTLL+PY+L +++++KCK+RV F+ + + + +E + + SLL KFR+ F +V IL D+ ++P+ E F+ M++ DE V P +S+ + K+ ++ +R+ EI + S +AL+V+TLP+ R+ + P +LY+AWL+ +S+ + PP + +RGNQE+VLTFY
Sbjct: 128 KNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPI--------NDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWR----GPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEAS--------------ARVDPKAL------------------------------VKEE---QATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRV-FVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFY 1019
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1717802|sp|P55019.1|S12A3_PSEAM (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter) HSP 1 Score: 687.952 bits (1774), Expect = 0.000e+0 Identity = 396/1038 (38.15%), Postives = 608/1038 (58.57%), Query Frame = 0
Query: 137 YLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDEL-------------LDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDC-EGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQ-QSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITI----PHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKM-PPFLMVRGNQDSVLT 1154
Y T + PH D Y N + G R RP+L +L +E+ + G D+ + + +FGW GV +RC+LNIWGV+L+LRL W+ QAG+ II LS+ +T IT +S SA++TNG++K GG Y++ISRSLGPE GG+IG++F AN++AV+M+ +GF E++ D++R+ NG+V V+ IND+R++G I++ +L +++ GMEW ++ Q+ L+++VS V++I+G++IP+S ++ AKGF Y + +N + E S FF +F++FFP+ TGI+AGAN+SGDLKDP VAIP+GTL+AI T ++Y+I + C +R ASG + + + + G + +C + C +GI+ Q M ++SA+ PLI AG F ATLSSA+A LV AP+V Q + KD+L+P I F +G+G N +P+R Y+L ++I++ +LI +LN ++ ++SNFF+ +YALINFS FHAS+T+SPGWRPSF++Y+KW+SLLG + C+ +MFL+ + A+I + L + ++KP VNWGSS Q+ S+ AL L +V+DHVKNYRP+ +VL+G P RP+L+D LTKRLSL+ + A + WLN ++SF+ L G L+ GLG++ PN++LMGFK+DW S A Y L AFD+ V VLR+ GLD S + F+ G ES +V +PA + Q SS+S +D D +P + F+ K + TIDIYWL DDGGLT+LLP++LT R ++A K+R+ F+ + D+ +E + AL+ KFR+ F DV +L D+ + D F+ + +PK Q S S + + E ++TE++L+R+ ++ Q+R+ E+++ +S EA L+V+T+P+ +R P L++AWLD +R + PP L+VRGNQ++VLT
Sbjct: 57 YDTVDAPPHYDFYANTEVW--GRRRRVRPSLYQLSVDPEQDDFKPPMYEETAGERMGGGDSSEEEEEEHKEPPPEPPRFGWVQGVMIRCMLNIWGVILYLRLPWITAQAGIGLTWVIILLSSFITGITGLSTSAIATNGKVKGGGTYFLISRSLGPELGGSIGLIFAFANAVAVAMHTVGFAETVTDLMRE-----NGVV-MVDPINDIRIVGVITVTCLLGISMAGMEWESKAQVLFFLVIMVSFVNYIVGTIIPASPQKQAKGFFSYKAEIFAANFVPGWRGKEGS-----FFGMFSIFFPSATGILAGANISGDLKDPTVAIPRGTLMAIFWTTISYLIISATIGACVVRDASGELNDTLSYSSSSENCSGLACQYRWDFSECIKNNTCKHGIMNYYQSMSLVSAFAPLISAGIFGATLSSALACLVSAPKVFQCLCKDQLYPLIGFFGKGYGKNAEPLRAYLLTYVIAVCFVLIAELNTIAPIISNFFLCSYALINFSCFHASVTNSPGWRPSFRFYSKWLSLLGAVCCVVIMFLLTWWAALIAFGVVFFLLGYTLYKKPAVNWGSSVQASSYSMALNQCVGLNQVEDHVKNYRPQCLVLTGPPCCRPALVDLVGCLTKRLSLMMCGHVVTAG-----PSPVSERHVTWLNQRKVRSFYRGVVAADLRSGVNMLLQGAGLGRIKPNVLLMGFKKDWGCDSPQAAHHYIGILHDAFDLHYGVCVLRVKEGLDASH------------------PPQCHVNPGFDGGPESINTV----CAPACV---------------QTSVTSSVS---MDPDPQPS--------SVFQKKQGKK-TIDIYWLSDDGGLTLLLPYLLTRRKRWAGCKVRV-FVGGDTDKKEEQKEEVLALIKKFRLGFHDVEVLPDIHQPPQPGNVDHFEDSVNRFRLETNPK--QDSDSGPQQQQ-----QEEPWMITEQDLERNRAKSLRQIRLNEVLQVHSREAALIVITMPVGRRGVCPSTLFLAWLDVLSRDLRPPVLLVRGNQENVLT 1019 HSP 2 Score: 645.966 bits (1665), Expect = 0.000e+0 Identity = 373/946 (39.43%), Postives = 558/946 (58.99%), Query Frame = 0
Query: 1200 RFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELL-----------LDREDELYFQNC-SNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWL-YPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS----------GDEQGVQRP------IVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSM-PPFLYVRGNQESVLTFYS 2115
RFGW+QGV + C+LNIWGV+L+LRL W+ QAG+ ++ II +++ +T +T +S SA+ TNG++KGGG YF+ISRSLGPE GG++GL+F A ++AV+M+ +GF E + D++ + +G+V ND+RI+G T+ LLGIS GM +K Q+L LV++ S V++++GT +P S +K+A G + ++ +N P ++ G++ F +F++FFP+ TG++AGAN+SGDLKDP AIP+GTL AI T SY++ + I A +R ASG + L L + F C N C +G + Q + L S + PLI AG F ATLSSALA LV P+VFQ + KD+L+P I FG+G+G++ EPLR YLL + IA+ +LI +LN ++ ++SNFF+ +YA+IN+S FHAS NSPGWRPSF++++KW+SLLG C+V+M L+ AL + L + + P VNWGSS Q+ S+ AL LN VEDHVKNYRP+ +V+TG P RPAL D +TKR SL+ HV+ P ++ WL +++FY GV ++ +GLG++ N+L++GF++ W P+ Y +L +FD+H V VLRV+ GLD S H +N + P+S+ V + T S VS D +P + K Q TID+YWL DDGGLTLLLPY+L+ RK++A CK+RV F+ D E+ E++ +L+ KFR+ F DV +L D+ + P+ + +F+ V+ + G Q+ +++E +L ++ ++ +R+ E+ + +S +AL+V+T+P+ R+ P TL+LAWLD +SR + PP L VRGNQE+VLTFY
Sbjct: 132 RFGWVQGVMIRCMLNIWGVILYLRLPWITAQAGIGLTWVIILLSSFITGITGLSTSAIATNGKVKGGGTYFLISRSLGPELGGSIGLIFAFANAVAVAMHTVGFAETVTDLMRE-----NGVV--MVDPINDIRIVGVITVTCLLGISMAGMEWESKAQVLFFLVIMVSFVNYIVGTIIPASPQKQAKGFFSYKAEIFAANFVPGWR----GKEGSFFGMFSIFFPSATGILAGANISGDLKDPTVAIPRGTLMAIFWTTISYLIISATIGACVVRDASGELNDTLSYSSSSENCSGLACQYRWDFSECIKNNTCKHGIMNYYQSMSLVSAFAPLISAGIFGATLSSALACLVSAPKVFQCLCKDQLYPLIGFFGKGYGKNAEPLRAYLLTYVIAVCFVLIAELNTIAPIISNFFLCSYALINFSCFHASVTNSPGWRPSFRFYSKWLSLLGAVCCVVIMFLLTWWAALIAFGVVFFLLGYTLYKKPAVNWGSSVQASSYSMALNQCVGLNQVEDHVKNYRPQCLVLTGPPCCRPALVDLVGCLTKRLSLMMCGHVVTAGPSPVSERHVT-----WLNQRKVRSFYRGVVAADLRSGVNMLLQGAGLGRIKPNVLLMGFKKDWGCDSPQAAHHYIGILHDAFDLHYGVCVLRVKEGLDASHPPQCH---------------------VNPGF---DGGPESIN----TVCAPACVQTSVTSSVSM---DPDPQPSSVF-------QKKQGKKTIDIYWLSDDGGLTLLLPYLLTRRKRWAGCKVRV-FVGGDTDKKEEQKEEVLALIKKFRLGFHDVEVLPDIHQPPQPGNVDHFEDSVNRFRLETNPKQDSDSGPQQQQQEEPWMITEQDLERNRAKSLRQIRLNEVLQVHSREAALIVITMPVGRRGVCPSTLFLAWLDVLSRDLRPPVLLVRGNQENVLTFYC 1022
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|676657102|gb|AIM43576.1| (Na+/K+/2Cl- cotransporter isoform 1, partial [Halocaridina rubra]) HSP 1 Score: 977.622 bits (2526), Expect = 0.000e+0 Identity = 525/1041 (50.43%), Postives = 716/1041 (68.78%), Query Frame = 0
Query: 132 KPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEIL--------NITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEE---YYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRT--TSELSEQSLNFEDGSESKMSVDSGLASP--ATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDK--DGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
K L+HYLT+E LP HYRN S G +RPTLD+L + + A+ + GK IKFGW +GVYMRCLLNIWGVMLFLR++W++GQ+G + L I+ L+N VT+IT++SMSAV+TNG+I+AGG+YYMISRSLGPEFGG+IG+MFT+ANSIA + YIIGFC+SL D+++ Y F+G + +ND RL+G I+L +L LAIVGM+WVTRVQM LL LL+ SQ+DFIIG++I P+ E A+GFVG +L N + Y E G + FFSVF VFF AVTGIVAGANLSGDLKDP+VAIP+GTL AI+TT++TYMIY +M+A ++R A+GN E + + D V I + + Y + + C +G++ S Q+ME++SAWGPLIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+PGIH FS+G+GANNDPVRGYILVF+IS CI+IGDLN VS+LLSNFF+A+Y LIN S FHA++ SPGWRPSFKYYN W+SL+G ++C+ VMFL+D+ TA+IT VC+I LY+F+ +R P VNWGSS+Q+Q+++TALKA L V++HVKNYRP+++VLSG+ RP L+DFA +TK +SLL + + + +AL + WL + I++F+S+ ++L +G+R ++ GLGKL PNMVL+G+K +W + D +E Y+ TL A DM L + +LR+ GLD S++ DE ++ N T T+ E N G S+ S +SP + +R D + +K K SLA L + G ++++ + IT F+ K + GTID++WLYDDGGLT+L+P+ILTTR++++ KLR+F L+ DELD + R+M LL+KFRI++SDVI++ D+ K+A + +R +F +I KS SE + K D E L+ E EL E+TN LR+ E++ +NS ++ LVVMTLP+P++ + PLYMAWL+ TR MPPFL++RGNQ SVLT
Sbjct: 56 KSLRHYLTREALPKESHYRNLNSIVDGS---ARPTLDDLHQNTYREQQHQGSADP--EAPQTKLRGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWMVGQSGWIFSLLIVILANSVTLITSLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTIANSIASATYIIGFCDSLKDLMKYY---FDGAIIIDGALNDTRLVGVITLCAVLGLAIVGMDWVTRVQMALLFLLIGSQIDFIIGAIIGPTDTLEEARGFVGMSVDLLRENGMSDYRVYE--GEKQGFFSVFGVFFTAVTGIVAGANLSGDLKDPSVAIPRGTLAAILTTFITYMIYPIMIAAGTVRDATGN-ETAYLQ---YKDNVSNIWENPTFTNESCYKGYGENDVPHCEFGLINSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIHIFSKGYGANNDPVRGYILVFVISFACIMIGDLNLVSTLLSNFFLASYCLINISCFHATLIKSPGWRPSFKYYNSWVSLIGGLMCLAVMFLIDWVTALITFVCVIGLYMFVSYRNPDVNWGSSTQAQTYVTALKATLDLISVEEHVKNYRPQLLVLSGSAGSRPPLLDFAQSITKNISLLSLGHVIQGPQTQRIRNALSRQSYNWLARHKIRAFYSLVEGNNLEDGSRNLFQLVGLGKLRPNMVLLGYKANWGK--CDRQELKAYFDTLHEALDMYLGIAILRVPQGLDYSQII-EDEDTPVVMNGNDTSITANTEEMKRNQSAGQLSQDDNGSEASSPPGSPTLERATNNDPAA---DEKAIKRRASLANLYRGPGGVELSKDVLSNITMFKRKQ-KKGTIDVWWLYDDGGLTLLVPYILTTRSQWSNCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKRAAESSRMEFDKLIEDFQAKS---------SEEISKED--EGTLICEAELLGQREKTNRHLRLRELLLENSKDSSLVVMTLPMPRKSSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLT 1064 HSP 2 Score: 840.877 bits (2171), Expect = 0.000e+0 Identity = 467/1027 (45.47%), Postives = 668/1027 (65.04%), Query Frame = 0
Query: 1134 FTTRKMPPFLMVRGNQDSVL--TSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDG-IVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDEL--YFQNCSNRN--------------CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEK--VMTAASQASGASDED-RFSMLNHALSLDEFQPK--------SVQLSSKNVDSFGTSSTRNNSEVS--YGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTM-----------VSGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+ TR+ P N +S++ ++RPTLD+L + G+AD +A T K +FGW++GVY+ CLLNIWGVMLFLR++W+VGQ+G + S I+ +AN VT +T++SMSAV TNG+I+ GG+Y+MISRSLGPEFGG++GLMFT A SIA + YIIGFC++L D++ + FDG I+ D ND R++G TL +LG++ +GM V ++Q+ LL +L+ SQ+DF+IG + P+D + A G VG DLLR N D+ + +GE+ F VF VFF AVTG+VAGAN+SGDLKDP AIP+GTLAAILTT+ +YM+Y ++IAA +R A+GN E L +D + ++N + N C +G + + Q+++L S WGPLIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P I F +G+G + +P+RGY+L+F I+ CI+IGDLN VS+LLSNFF+A+Y +IN S FHA+ SPGWRPSFKY+N W+SL+G +C+ VM L+D TAL T +C+I LY+FV RNPDVNWGSS+Q+Q+++TALK+ L VE+HVKNYRP+L+V++G+ RP L DFA ITK SLL L HVI+ RI + + ++WL + I+AFY+L ++ G R +L GLGKL NM+++G++ W + + Y+ L + DM+L + +LRV GLD S I + VM + A+ E+ + + LS D+ + +++ ++ N + + + + ++ Y G + + ++ +T F+ K QK GTIDV+WLYDDGGLTLL+PYIL+TR +++ CK+RVF L+NR D L E MA+LL KFRI++ DVI++ DV K + + F + +S +++G ++ E LL ++T HLR+ E+ ENS S+LVVMTLP+PR++ + LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct: 61 YLTREALPKESHYRNLNSIVDGSARPTLDDLHQNTYR--------EQQHQGSADPEAPQT-KLRGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWMVGQSGWIFSLLIVILANSVTLITSLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTIANSIASATYIIGFCDSLKDLMKYY---FDGAIIIDGA--LNDTRLVGVITLCAVLGLAIVGMDWVTRVQMALLFLLIGSQIDFIIGAIIGPTDTLEEARGFVGMSVDLLRENGMSDY-RVYEGEKQGFFSVFGVFFTAVTGIVAGANLSGDLKDPSVAIPRGTLAAILTTFITYMIYPIMIAAGTVRDATGN-ETAYLQYKDNVSNIWENPTFTNESCYKGYGENDVPHCEFGLINSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIHIFSKGYGANNDPVRGYILVFVISFACIMIGDLNLVSTLLSNFFLASYCLINISCFHATLIKSPGWRPSFKYYNSWVSLIGGLMCLAVMFLIDWVTALITFVCVIGLYMFVSYRNPDVNWGSSTQAQTYVTALKATLDLISVEEHVKNYRPQLLVLSGSAGSRPPLLDFAQSITKNISLLSLGHVIQGPQTQRIRNALSRQSYNWLARHKIRAFYSLVEGNNLEDGSRNLFQLVGLGKLRPNMVLLGYKANWGKCDRQELKAYFDTLHEALDMYLGIAILRVPQGLDYSQIIEDEDTPVVMNGNDTSITANTEEMKRNQSAGQLSQDDNGSEASSPPGSPTLERATNNDPAADEKAIKRRASLANLYRGPGGVELSKDVLSNITMFKRK-QKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSNCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKRAAESSRMEFDKLIEDFQAKSSEEISKEDEGT---LICEAELLGQREKTNRHLRLRELLLENSKDSSLVVMTLPMPRKSSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLTFYS 1067
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|676657122|gb|AIM43577.1| (Na+/K+/2Cl- cotransporter isoform 1, partial [Halocaridina rubra]) HSP 1 Score: 977.237 bits (2525), Expect = 0.000e+0 Identity = 525/1041 (50.43%), Postives = 716/1041 (68.78%), Query Frame = 0
Query: 132 KPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEIL--------NITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEE---YYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRT--TSELSEQSLNFEDGSESKMSVDSGLASP--ATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDK--DGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
K L+HYLT+E LP HYRN S G +RPTLD+L + + A+ + GK IKFGW +GVYMRCLLNIWGVMLFLR++W++GQ+G + L I+ L+N VT+IT++SMSAV+TNG+I+AGG+YYMISRSLGPEFGG+IG+MFT+ANSIA + YIIGFC+SL D+++ Y F+G + +ND RL+G I+L +L LAIVGM+WVTRVQM LL LL+ SQ+DFIIG++I P+ E A+GFVG +L N + Y E G + FFSVF VFF AVTGIVAGANLSGDLKDP+VAIP+GTL AI+TT++TYMIY +M+A ++R A+GN E + + D V I + + Y + + C +G++ S Q+ME++SAWGPLIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+PGIH FS+G+GANNDPVRGYILVF+IS CI+IGDLN VS+LLSNFF+A+Y LIN S FHA++ SPGWRPSFKYYN W+SL+G ++C+ VMFL+D+ TA+IT VC+I LY+F+ +R P VNWGSS+Q+Q+++TALKA L V++HVKNYRP+++VLSG+ RP L+DFA +TK +SLL + + + +AL + WL + I++F+S+ ++L +G+R ++ GLGKL PNMVL+G+K +W + D +E Y+ TL A DM L + +LR+ GLD S++ DE ++ N T T+ E N G S+ S +SP + +R D + +K K SLA L + G ++++ + IT F+ K + GTID++WLYDDGGLT+L+P+ILTTR++++ KLR+F L+ DELD + R+M LL+KFRI++SDVI++ D+ K+A + +R +F +I KS SE + K D E L+ E EL E+TN LR+ E++ +NS ++ LVVMTLP+P++ + PLYMAWL+ TR MPPFL++RGNQ SVLT
Sbjct: 56 KSLRHYLTREALPKESHYRNLNSIVDGS---ARPTLDDLHQNTYREQQHQGSADP--EAPQTKLRGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWMVGQSGWIFSLLIVILANSVTLITSLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTIANSIASATYIIGFCDSLKDLMKYY---FDGAIIIDGALNDTRLVGVITLCAVLGLAIVGMDWVTRVQMALLFLLIGSQIDFIIGAIIGPTDTLEEARGFVGMSVDLLRENGMSDYRVYE--GEKQGFFSVFGVFFTAVTGIVAGANLSGDLKDPSVAIPRGTLAAILTTFITYMIYPIMIAAGTVRDATGN-ETAYLQ---YKDNVSNIWENPTFTNESCYKGYGENDVPHCEFGLINSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIHIFSKGYGANNDPVRGYILVFVISFACIMIGDLNLVSTLLSNFFLASYCLINISCFHATLIKSPGWRPSFKYYNSWVSLIGGLMCLAVMFLIDWVTALITFVCVIGLYMFVSYRNPDVNWGSSTQAQTYVTALKATLDLISVEEHVKNYRPQLLVLSGSAGSRPPLLDFAQSITKNISLLSLGHVIQGPQTQRIRNALSRQSYNWLARHKIRAFYSLVEGNNLEDGSRNLFQLVGLGKLRPNMVLLGYKANWGK--CDRQELKAYFDTLHEALDMYLGIAILRVPQGLDYSQII-EDEDTPVVMNGNDTSITANTEEMKRNQSAGQLSQDDNGSEASSPPGSPTLERATNNDPAA---DEKAIKRRASLANLYRGPGGVELSKDVLSNITMFKRKQ-KKGTIDVWWLYDDGGLTLLVPYILTTRSQWSNCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKRAAESSRMEFDKLIEDFQAKS---------SEEISKED--EGTLICEAELLGQREKTNRHLRLRELLLENSKDSSLVVMTLPMPRKSSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLT 1064 HSP 2 Score: 840.877 bits (2171), Expect = 0.000e+0 Identity = 467/1027 (45.47%), Postives = 668/1027 (65.04%), Query Frame = 0
Query: 1134 FTTRKMPPFLMVRGNQDSVL--TSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDG-IVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDEL--YFQNCSNRN--------------CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEK--VMTAASQASGASDED-RFSMLNHALSLDEFQPK--------SVQLSSKNVDSFGTSSTRNNSEVS--YGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTM-----------VSGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+ TR+ P N +S++ ++RPTLD+L + G+AD +A T K +FGW++GVY+ CLLNIWGVMLFLR++W+VGQ+G + S I+ +AN VT +T++SMSAV TNG+I+ GG+Y+MISRSLGPEFGG++GLMFT A SIA + YIIGFC++L D++ + FDG I+ D ND R++G TL +LG++ +GM V ++Q+ LL +L+ SQ+DF+IG + P+D + A G VG DLLR N D+ + +GE+ F VF VFF AVTG+VAGAN+SGDLKDP AIP+GTLAAILTT+ +YM+Y ++IAA +R A+GN E L +D + ++N + N C +G + + Q+++L S WGPLIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P I F +G+G + +P+RGY+L+F I+ CI+IGDLN VS+LLSNFF+A+Y +IN S FHA+ SPGWRPSFKY+N W+SL+G +C+ VM L+D TAL T +C+I LY+FV RNPDVNWGSS+Q+Q+++TALK+ L VE+HVKNYRP+L+V++G+ RP L DFA ITK SLL L HVI+ RI + + ++WL + I+AFY+L ++ G R +L GLGKL NM+++G++ W + + Y+ L + DM+L + +LRV GLD S I + VM + A+ E+ + + LS D+ + +++ ++ N + + + + ++ Y G + + ++ +T F+ K QK GTIDV+WLYDDGGLTLL+PYIL+TR +++ CK+RVF L+NR D L E MA+LL KFRI++ DVI++ DV K + + F + +S +++G ++ E LL ++T HLR+ E+ ENS S+LVVMTLP+PR++ + LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct: 61 YLTREALPKESHYRNLNSIVDGSARPTLDDLHQNTYR--------EQQHQGSADPEAPQT-KLRGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWMVGQSGWIFSLLIVILANSVTLITSLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTIANSIASATYIIGFCDSLKDLMKYY---FDGAIIIDGA--LNDTRLVGVITLCAVLGLAIVGMDWVTRVQMALLFLLIGSQIDFIIGAIIGPTDTLEEARGFVGMSVDLLRENGMSDY-RVYEGEKQGFFSVFGVFFTAVTGIVAGANLSGDLKDPSVAIPRGTLAAILTTFITYMIYPIMIAAGTVRDATGN-ETAYLQYKDNVSNIWENPTFTNESCYKGYGENDVPHCEFGLINSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIHIFSKGYGANNDPVRGYILVFVISFACIMIGDLNLVSTLLSNFFLASYCLINISCFHATLIKSPGWRPSFKYYNSWVSLIGGLMCLAVMFLIDWVTALITFVCVIGLYMFVSYRNPDVNWGSSTQAQTYVTALKATLDLISVEEHVKNYRPQLLVLSGSAGSRPPLLDFAQSITKNISLLSLGHVIQGPQTQRIRNALSRQSYNWLARHKIRAFYSLVEGNNLEDGSRNLFQLVGLGKLRPNMVLLGYKANWGKCDRQELKAYFDTLHEALDMYLGIAILRVPQGLDYSQIIEDEDTPVVMNGNDTSITANTEEMKRNQSAGQLSQDDNGSEASSPPGSPTLERATNNDPAADEKAIKRRASLANLYRGPGGVELSKDVLSNITMFKRK-QKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSNCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKRAAESSRMEFDKLIEDFQAKSSEEISKEDEGT---LICEAELLGQREKTNRHLRLRELLLENSKDSSLVVMTLPMPRKSSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLTFYS 1067
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|6179898|gb|AAF05702.1|AF190129_1 (Na+/K+/2Cl- cotransporter [Callinectes sapidus]) HSP 1 Score: 974.541 bits (2518), Expect = 0.000e+0 Identity = 514/1050 (48.95%), Postives = 716/1050 (68.19%), Query Frame = 0
Query: 129 LTPKPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSS---EGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCE--------GRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARN-----RTTSELSEQSLN---FEDGSESKMSVDSGLASPATLTKRKLGRDGSVL---GRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
L + L + LP HYRN S G RPTLD+L + + N ++G++ AE ++ GK IKFGW +GVYMRCLLNIWGVMLFLR++WV+GQAG++ L + L NIVT +TT+SMSAV+TNG+I+AGG+YYMISRSLGPEFGG+IG+MFTLANSIA + YIIGFC+SL D++ Y D IVD +ND R++G+I+L+ +LALAIVGM+WVTRVQMGLL LL+ SQ+DF++G+ I P D + ++GF+G+ V+ N+ Y E G NFFSVF VFF AVTGIVAGANLSGDLKDPA AIPKGTL AI+TT+ TY+IY +M+ +R A+G+ ++V+ N ++ E A+ +C + C +G+ S Q+ME++SAWGPLIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+PGI FS+G GANNDPVRGYILVF+IS +CI+IGDLN VS+LLSNFF+A+Y+LINFS FHAS+ SPGWRPSFKYYN WIS LG ILC+ VMFL+D+ TA+ T + II LY+F+ +R P VNWGSS+Q+Q++++ALK L +++HVKNYRP+I+VL+G RP L+DF++ +TK +SLL + + + ++L + WL + I++F+S+ +L +GAR ++ GLGKL PN V++G+K +W++ + + Y+ TL A DM V +LR+ GLD S++ DE + + N +TT + QS +DG++S+ S G SP ++ R +V+ G KK ++S++ G ++++ + IT F+ K + GTID++WLYDDGGLT+L+P+ILTTR++++ KLR+F L+ DELD + R+M LL+KFRI++SDVI++ D+ KKA + +R +F +I + + VDK + +L++E EL E+TN +R+ E++ +NS ++ LVVMTLP+P++ + PLYMAWL+ TR MPPFL++RGNQ SVLT
Sbjct: 20 LATRSLCAIILPRALPKESHYRNLDSIVDG--SGPRPTLDDLHHNT----LRENQRQQGIDDAEAATANPSGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQAGIILALMTVILGNIVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLKDLMFYYF-DGAKIVDGA--VNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQDDLQRSQGFIGFSGEVMAKNVGPDYRDFENRG--QNFFSVFGVFFTAVTGIVAGANLSGDLKDPADAIPKGTLAAILTTFCTYIIYPIMIGAAVLRDATGD-KDVYLMYQ--NHSIDE----NPAFTNCSLTGSVDNGTQVCKFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYILVFVISFVCIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWLGGILCLIVMFLIDWITALATFLIIIALYLFVSYRNPNVNWGSSTQAQTYVSALKTALDLNTIEEHVKNYRPQILVLTGPVGSRPPLIDFSYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLTRHSIRAFYSLVEGSTLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCEPKELKAYFNTLHEALDMYFGVVILRVPQGLDYSQII-EDEDSPITMNGNEGAITQTTDDKPGQSSANQLTQDGTDSEASSPPG--SP------QVERSAAVVDANGENSKKRRTSLANLFRGPGGSELSKDVLNNITMFKRKQ-KKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLI-----------EDFKTKDEVDKES--DGLLISEAELLGQREKTNRHIRLRELLLENSRDSTLVVMTLPMPRKTSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLT 1028 HSP 2 Score: 877.47 bits (2266), Expect = 0.000e+0 Identity = 460/1007 (45.68%), Postives = 660/1007 (65.54%), Query Frame = 0
Query: 1148 NQDSVLTS---RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDG--IVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDEL----YFQNCS--------NRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWW-LYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDI----------SGAHEKVMTAASQASGASDEDRFSM--LNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIR--DGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV----SGDEQGVQRP--IVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
N DS++ RPTLD+L + Q G D +A T +FGW++GVY+ CLLNIWGVMLFLR++WVVGQAG++ + + + N+VT++T +SMSAV TNG+I+ GG+Y+MISRSLGPEFGG++GLMFT A SIA + YIIGFC++L D+++ + FDG IV + ND RI+G+ TL+ +L ++ +GM V ++Q+ LL +L+ SQ+DF++G F+ P D+ +R+ G +GF +++ N+ PD+ ++ + F VF VFF AVTG+VAGAN+SGDLKDP AIPKGTLAAILTT+C+Y++Y ++I A+ +R A+G+ + L+ + + F NCS + C +G + Q+++L S WGPLIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P I F +G G + +P+RGY+L+F I+ CI+IGDLN VS+LLSNFF+A+Y++IN+S FHAS SPGWRPSFKY+N WIS LG LC++VM L+D TALAT + II LY+FV RNP+VNWGSS+Q+Q++++ALK+ LN +E+HVKNYRP+++V+TG RP L DF+ ITK SLL HVI+ R+ +++ ++WL + I+AFY+L + G R +L GLGKL N +++G++ W PK + Y+ L + DM+ V +LRV GLD S I +G + G S ++ + + S P+ V+ S+ VD+ G +S + + ++ R G+ + + ++ +T F+ K QK GTIDV+WLYDDGGLTLL+PYIL+TR +++ CK+RVF L+NR D L E MA+LL KFRI++ DVI++ DV K + + F ++ + DE + ++SE LL ++T H+R+ E+ ENS S LVVMTLP+PR+ + LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct: 42 NLDSIVDGSGPRPTLDDLHHNT-------LRENQRQQGIDDAEAA-TANPSGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQAGIILALMTVILGNIVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLKDLMFYY---FDGAKIV---DGAVNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQDDLQRSQGFIGFSGEVMAKNVGPDY-RDFENRGQNFFSVFGVFFTAVTGIVAGANLSGDLKDPADAIPKGTLAAILTTFCTYIIYPIMIGAAVLRDATGDKDVYLMYQNHSIDENPAFTNCSLTGSVDNGTQVCKFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYILVFVISFVCIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWLGGILCLIVMFLIDWITALATFLIIIALYLFVSYRNPNVNWGSSTQAQTYVSALKTALDLNTIEEHVKNYRPQILVLTGPVGSRPPLIDFSYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLTRHSIRAFYSLVEGSTLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCEPKELKAYFNTLHEALDMYFGVVILRVPQGLDYSQIIEDEDSPITMNGNEGAITQTTDDKPGQSSANQLTQDGTDSEASSPPGSPQ-VERSAAVVDANGENSKKRRTSLANLFRGPGGSELSKDVLNNITMFKRK-QKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKTKDEVDKESDGLLISEAELLGQREKTNRHIRLRELLLENSRDSTLVVMTLPMPRKTSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLTFYS 1031
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|242016228|ref|XP_002428731.1| (sodium-potassium-chloride cotransporter, putative [Pediculus humanus corporis] >gi|212513416|gb|EEB15993.1| sodium-potassium-chloride cotransporter, putative [Pediculus humanus corporis]) HSP 1 Score: 968.378 bits (2502), Expect = 0.000e+0 Identity = 503/1044 (48.18%), Postives = 714/1044 (68.39%), Query Frame = 0
Query: 116 QRKMTSKQRSSFILTPKPLKHYL--TKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQY-LTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
+ T+ ++++ PK K++L T+E LP ++HY++ F+ H RP+LD L ++ + E+ +GK IKFGW DGV+MRCLLNIWGVMLFLRL+WV+GQAG+ EG II L+N+VT++TTISMSAV TNGQIK GG+YYMISRSLGP+FGGAIG+MFTLANS+AV+MYI+GFCESL +L+ + L +G +ND+RL+G+++L+LIL LAIVGM+WVTR QM LL++L++SQ+DF+IG+++ P + EEIAKGFVGY+ + N +++Y E G++H+FFSVFAVFFPAVTGIVAGANLSGDLKDP+ AIPKGTLLAI T+++Y+ Y MV C +R ASG+VE+ G + N +L +T +C C+YG+ Q ME+ S WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I FSEG+GANNDPVRGY+LVF S CILI LN ++ LLSNFF+ AYALINFSVFHASI+ SPGWRP+FKYYN W+SLLGT+LCI VMFL+ + TA+IT V ++ LY+++ +RKP VNWGSS+Q+Q + AL++V L +++HVKNYRP+I++LSG P RP L+ F +L+ K LSLL S I + +++D L + WL + +K F ++ + GAR+ ++ G+GKL PNM+LMG+K W++ SL + +Y+ + A D LA+G+LR+ GLD S ++ ++ N + +++ D + S + S L S RF+ + LD DG PV+++ ++ + FR + +G ID++WLYDDGGLT+L+P+IL+TR+ F++ +LR+F L N DELD++ R + ALLSKFRI DVI++ D+ KKA +R +F ++ + Q+++ + +++++++ E E++N +R+ E++R++SS+A +VMTLP+P++ + P+YMAWL+ T+ MPP+L+VRGNQ+SVLT
Sbjct: 74 KHGQTTNHNNTYVYDPKISKNFLEYTREALPRLEHYKD---FNSVH-HAQRPSLDALHNDRFHEKVQREKDEE-----NSEPQGKIIKFGWLDGVFMRCLLNIWGVMLFLRLSWVVGQAGIGEGALIICLANVVTIVTTISMSAVCTNGQIKGGGVYYMISRSLGPQFGGAIGLMFTLANSVAVAMYIVGFCESLQTLLKTFDLIIIDG------DVNDIRLVGTLTLILILILAIVGMDWVTRTQMVLLIVLILSQLDFVIGTIVGPKNQEEIAKGFVGYNGELFTKNFFSSYQLHE--GIEHDFFSVFAVFFPAVTGIVAGANLSGDLKDPSSAIPKGTLLAIFVTFLSYLAYAFMVGGCVLRDASGDVEDYIKGLNESN----PLLYVT----NCTIGSCSYGLQNDSQAMELASLWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSEGYGANNDPVRGYVLVFCTSFACILIAQLNAIAPLLSNFFLTAYALINFSVFHASISKSPGWRPAFKYYNAWVSLLGTLLCILVMFLISWWTALITFVVVVTLYLYVSYRKPDVNWGSSTQAQCYSIALRSVIDLNYMEEHVKNYRPQIVILSGLPGWRPPLVHFGNLVVKGLSLLISSHIVPEEVSQREIDNLTKRGHRWLAHHKVKGFFNVVAAKNFESGARSLMQNVGVGKLRPNMLLMGYKSTWKKCSLDELLQYFNVIHGALDRHLAIGILRMPSGLDYSNRIQDED---LVAPENERERKRRKRNKKLIDRNNSSNHLSSVLRS-----------------RFKGTIVPKVERQPLDLDGNPVSKDVLDSLMHFRKRQT-AGNIDVWWLYDDGGLTLLIPYILSTRSIFSECRLRVFSLCSNADELDQEQRKLAALLSKFRISCKDVIVIPDIMKKAQTSSRIEFDGLVQNFRVRDDQVTEEN------------KSIVISDTEYHNLREKSNRHMRLRELLREHSSDAAFIVMTLPVPRKGLVSAPMYMAWLELLTKGMPPYLLVRGNQNSVLT 1059 HSP 2 Score: 840.106 bits (2169), Expect = 0.000e+0 Identity = 448/1003 (44.67%), Postives = 646/1003 (64.41%), Query Frame = 0
Query: 1131 WLDFTTRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDE---LYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGE--EYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDE-----DRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSG----DEQGVQRP---IVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
+L++T +P + RP+LD L N + R ++ Q K +FGW+ GV++ CLLNIWGVMLFLRL+WVVGQAG+ II +AN+VT VT ISMSAVCTNGQIKGGG+Y+MISRSLGP+FGGA+GLMFT A S+AV+MYI+GFCE+L ++ FD I+ D + ND+R++G+ TL+L+L ++ +GM V + Q++LL+VL+ SQ+DF+IGT V P + E+ A G VG++ +L N + +Q + +G + F VFAVFFPAVTG+VAGAN+SGDLKDP SAIPKGTL AI T+ SY+ YA ++ +R ASG+ E+ + + LY NC+ +C+YG + Q ++L+S+WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P+I F G+G + +P+RGY+L+F + CILI LN ++ LLSNFF+ YA+IN+SVFHAS + SPGWRP+FKY+N W+SLLGT LCI+VM L+ TAL T + ++ LY++V R PDVNWGSS+Q+Q + AL+S+ LN++E+HVKNYRP++++++G P RP L F NL+ K SLL H++ E+V R +DN+ H WL + +K F+ + +F G R+ ++ G+GKL NML++G++ W E +Y+ V+ + D HL++G+LR+ GLD S+ + V + DR + NH S+ L S+ + R ++ DGNP+ + ++ + FR K Q G IDV+WLYDDGGLTLL+PYILSTR F++C++RVF L + D L +E K+A+LL KFRI +DVI++ D+ K + + F +V D+Q + ++S+ +++ H+R+ E+ +E+S +A +VMTLP+PR+ + +Y+AWL+ +++ MPP+L VRGNQ SVLTFYS
Sbjct: 95 FLEYTREALPRLEHYKDFNSVHHAQRPSLDAL----HNDRFHEKVQREKDEENSEPQGKII--------KFGWLDGVFMRCLLNIWGVMLFLRLSWVVGQAGIGEGALIICLANVVTIVTTISMSAVCTNGQIKGGGVYYMISRSLGPQFGGAIGLMFTLANSVAVAMYIVGFCESLQTLL----KTFDLIIIDGD--VNDIRLVGTLTLILILILAIVGMDWVTRTQMVLLIVLILSQLDFVIGTIVGPKNQEEIAKGFVGYNGELFTKNFFSSYQLH-EGIEHDFFSVFAVFFPAVTGIVAGANLSGDLKDPSSAIPKGTLLAIFVTFLSYLAYAFMVGGCVLRDASGDVEDYIKGLNESNPLLYVTNCTIGSCSYGLQNDSQAMELASLWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSEGYGANNDPVRGYVLVFCTSFACILIAQLNAIAPLLSNFFLTAYALINFSVFHASISKSPGWRPAFKYYNAWVSLLGTLLCILVMFLISWWTALITFVVVVTLYLYVSYRKPDVNWGSSTQAQCYSIALRSVIDLNYMEEHVKNYRPQIVILSGLPGWRPPLVHFGNLVVKGLSLLISSHIVPEEVSQREIDNLTKRGHRWLAHHKVKGFFNVVAAKNFESGARSLMQNVGVGKLRPNMLLMGYKST-WKKCSLDELLQYFNVIHGALDRHLAIGILRMPSGLDYSNRIQDEDLVAPENERERKRRKRNKKLIDRNNSSNHLSSV---------LRSRFKGTIVPKVERQPLDL-----DGNPVSKDVLDSLMHFR-KRQTAGNIDVWWLYDDGGLTLLIPYILSTRSIFSECRLRVFSLCSNADELDQEQRKLAALLSKFRISCKDVIVIPDIMKKAQTSSRIEFDGLVQNFRVRDDQVTEENKSIVISDTEYHNLREKSNRHMRLRELLREHSSDAAFIVMTLPVPRKGLVSAPMYMAWLELLTKGMPPYLLVRGNQNSVLTFYS 1062
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1228009303|ref|XP_021936564.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X3 [Zootermopsis nevadensis] >gi|646699945|gb|KDR10324.1| Bumetanide-sensitive sodium-(potassium)-chloride cotransporter [Zootermopsis nevadensis]) HSP 1 Score: 966.837 bits (2498), Expect = 0.000e+0 Identity = 513/967 (53.05%), Postives = 667/967 (68.98%), Query Frame = 0
Query: 210 MRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEE---YYRTLETAFDMRLAVGVLRIDGGLDISEMFASDE----------KAQMIFARNRTTSELSEQSLNF--------EDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
MRCLLNIWGVMLFLRL+WVIGQAG+ EG II L+NIVT++T+ISMSAV TNGQIK GGIYYMISRSLGPEFGGAIG+MFTLANS+AVSMYI+GFCESL D+LR + T I+D ND+R++G ++L+ I LAIVGM+WVTR QM LL +L+ SQ+DF+IGS + P DEEI KGFVGY+ SNLY Y E GV H+FFSVF+VFFPAVTGIVAGANLSGDLKDP+ AIP GTLLAIV T+++Y+ YG M+ C +R+ASGNV E + GE N + +C R C YG+ Q ME+ S+WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I FS+G GANNDPVRGYILVF+IS CILI LN ++ LLSNFF+AAYALINFSVFHASI+ SPGWRP+FKYYN W+SL+GT+LCI VMFLM + A+ T V +I LY+++ +RKP+VNWGSS+Q+ S+ +ALKA+ L RV++HVKN+RP+++VL+G P RP L+DFA+L+TK LSLL I +A+ + +AL A +WL + +K+F IT +DS G + ++I GLGKL PNM+LMG+K +W+ D EE Y+ + A D LAVG+LR+ GLD S + +E A+ RN+++++L Q +N D M+ D +AS + L S R KT+ S + G P+ + + ITQF+ K + G ID++WLYDDGGLT+L+P+IL+TR++F+ +LRIF L+ D+LD D RN+ ALLSKFRI++SDV+++ D+ KKA TR +F+ I K G S +S S +TE EL E+TN +RI E++ Q S E+ +VM LP+P+++ + PLYMAWL+ T+ MPP L++RGNQ SV+T
Sbjct: 1 MRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRSFGTT---IIDGAS--NDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNLYPDYRNYE--GVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTE-------YLQVKGEP-NEWTIFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRT--CDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDP-VASDSLLESSTTVTVTSKKSRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTS---------NKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGMPPCLIIRGNQTSVIT 939 HSP 2 Score: 855.899 bits (2210), Expect = 0.000e+0 Identity = 451/951 (47.42%), Postives = 628/951 (66.04%), Query Frame = 0
Query: 1211 CLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF-DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLL---DREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLY-PKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDIS--------------DISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPK----------------SVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS------GDEQGV----QRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
CLLNIWGVMLFLRL+WV+GQAG+ AII +AN+VT VT+ISMSAVCTNGQIKGGGIY+MISRSLGPEFGGA+GLMFT A S+AVSMYI+GFCE+L D++ F + DG +ND+R++G TL+ + ++ +GM V + Q+ LL VL+ASQ+DF+IG+F+ P DE+ G VG++ + SNL+PD+ +N +G F VF+VFFPAVTG+VAGAN+SGDLKDP SAIP GTL AI+ T+ SY+ Y ++ +R ASGN E L + + F NC++R C YG + Q ++L+S WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P I F +G G + +P+RGY+L+F I+ CILI LN ++ LLSNFF+A YA+IN+SVFHAS + SPGWRP+FKY+N W+SL+GT LCI VM LM AL T + +I LY++V R P+VNWGSS+Q+ S+ +ALK++ LN VE+HVKN+RP+++V+TG P RP L DFA LITK SLL H++K R+ + + + WL + +KAF+ +T +DSF G + +++SGLGKL NM+++G++ W P+ ++Y+ V+ + D +L+VG+LRVQ GLD S D A EK+ S S + S+L+ AL+ D P +V ++SK S T T+ ++ Y G P+ + I+ +TQF+ K K G IDV+WLYDDGGLTLL+PYILSTR +F+ C++R+F L+++ D L + +A+LL KFRI++ DV+I+SD+ K T F+T + GD + ++E LL ++T H+RI E+ + S S +VM LP+PR++ + LY+AWL+ +++ MPP L +RGNQ SV+T YS
Sbjct: 3 CLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRSFGTTIIDG-------ASNDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNLYPDY-RNYEGVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTEYLQVKGEPNEWTIFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRTCDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDPVASDSLLESSTTVTVTSKK--SRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGMPPCLIIRGNQTSVITVYS 942
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1233209917|ref|XP_022204235.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Nilaparvata lugens]) HSP 1 Score: 955.666 bits (2469), Expect = 0.000e+0 Identity = 513/1040 (49.33%), Postives = 700/1040 (67.31%), Query Frame = 0
Query: 138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGV----NGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSIT--RNDSLLE-GARAALEIGGLGKLSPNMVLMGFKQDWQQS-LADAEEYYRTLETAFDMRLAVGVLRIDGGLDISE--------MFASDEK--AQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILD----KDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
LT+EVLP +HY++ S RPTL+EL + L +GV + +GK IKFGW +GVYMRCLLNIWGVMLFLRL+WVIGQAG+ G +I L+NIVT+ TT+SMSAVSTNG I+AGGIYYMISRSLGPEFGGAIG+MFTLAN++AVSMYI+GFCES+ DMLR + I+D+ ND+R++G ++LV IL LA VGMEWVTR QM LL++L+ SQ+DF++GS++ P + E++KGFVGYD V + NL Y E GV+HNFFSVF+VFFPAVTGIVAGANLSGDLKDP+ AIP+GTLLAI+TT+V+Y+ Y LM+ C +R ASGNV + D + I+Q C YG+ Q ME++S WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I+ FS+G+GANNDP+RGYILVF I+ C+LI LN ++ LLSNFF+AAYALINFSVFHASI+ PGWRP+FKYYN W+SL+GT+LCI VMFL+ + TA++T ++ LY+++ +RKP+VNWGSS+Q+QS+ AL++ L RV +HVKNYRP+++ LSG+P RP+++DFA+L+TK SLL I + + + DAL A WL+ + IK+F+++T R E A A +++ GLGKL PN VLMGFK +W L + Y+ + A D LAVG+L ++ GLD S + +S +K A+ RN++ +LS DG +S ++ + A + G V + K ++SS+S + D G P+ ++ IT+FR K + G ID++WL+DDGGLT+L+PHIL TR++FA SKLRIF L+ +LD D +N+ LL KFRI++SD+ ++ D+ K A +TR F+ +I + D A + ++T+ EL E+TN LR+ E++ ++S ++ L++MTLP+P++ + PLY++WL+ + +PP L++RGNQ SVLT
Sbjct: 75 LTREVLPRAEHYKDITSVHHAQ----RPTLEELHEARFLEKDQRRKKVEGVVEEDKSGANHKKGKVIKFGWVEGVYMRCLLNIWGVMLFLRLSWVIGQAGVGMGFAVIFLANIVTLATTLSMSAVSTNGLIEAGGIYYMISRSLGPEFGGAIGLMFTLANAVAVSMYIVGFCESVQDMLRLFGMS---IIDN--STNDIRVVGVVTLVCILILAFVGMEWVTRTQMVLLIVLIASQIDFVVGSIMGPKTLTEMSKGFVGYDLEVFKKNLVEDYRYFE--GVEHNFFSVFSVFFPAVTGIVAGANLSGDLKDPSKAIPRGTLLAILTTFVSYLSYALMMGGCVLRDASGNVTALREALDSGLAPPALYMTISQTCLGNNSAACRYGLHNDNQAMELVSVWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIYFFSKGYGANNDPIRGYILVFCIAFGCVLIAQLNAIAPLLSNFFLAAYALINFSVFHASISKCPGWRPAFKYYNAWVSLIGTLLCIAVMFLISWWTALVTFFVVVTLYLYVSYRKPEVNWGSSTQAQSYNFALRSALELNRVQEHVKNYRPQVLALSGDPGSRPAMIDFANLITKGNSLLVCGHILKGNHSQRVHDALTQKAQRWLDQHRIKAFYAVTSERTGGTFETSAEALMQLTGLGKLKPNTVLMGFKHNWHTCHLDELVNYFNLIHVALDSHLAVGILCLENGLDYSNYVQDKDVILISSLDKKDAEEKLRRNQSAGQLS------LDGEQSDIA-----PAAAAVNTHPDPNGGDVTQQLDKDSESSVSESGGDLFVGPSGEPLAPNLLKDITRFRRKQDK-GFIDVWWLFDDGGLTLLVPHILRTRSQFADSKLRIFSLASRKSQLDHDQKNLAQLLLKFRIDYSDLTVISDINKPARSETRLQFENLI------------EKFRLRGNDPAKADGSKIITDNELVLLHEKTNRHLRLRELLFEHSLDSTLIIMTLPMPRKGTVSAPLYLSWLETLSHGLPPTLILRGNQTSVLT 1079 HSP 2 Score: 827.395 bits (2136), Expect = 0.000e+0 Identity = 443/999 (44.34%), Postives = 644/999 (64.46%), Query Frame = 0
Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSD-EKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEEL--LLDR---EDELYF---QNCSNRN---CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRN---DSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEE---YYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLD----EFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIR----------DGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGDEQGVQRP-------IVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RPTL+EL + + R G + K +FGW++GVY+ CLLNIWGVMLFLRL+WV+GQAG+ FA+I +AN+VT T +SMSAV TNG I+ GGIY+MISRSLGPEFGGA+GLMFT A ++AVSMYI+GFCE++ DM+ F I+ +S ND+R++G TLV +L ++F+GM V + Q++LL+VL+ASQ+DF++G+ + P + + G VG+D ++ + NL D+ + +G + F VF+VFFPAVTG+VAGAN+SGDLKDP AIP+GTL AILTT+ SY+ YAL++ +R ASGN L LD LY Q C N C YG + Q ++L S+WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P+I F +G+G + +P+RGY+L+F IA C+LI LN ++ LLSNFF+A YA+IN+SVFHAS + PGWRP+FKY+N W+SL+GT LCI VM L+ TAL T ++ LY++V R P+VNWGSS+Q+QS+ AL+S LN V++HVKNYRP+++ ++G+P RPA+ DFANLITK +SLL H++K + R+ D + WL + IKAFYA+T +F ++L+GLGKL N +++GF+ W + +E Y+ ++ ++ D HL+VG+L ++ GLD S+ + ++ ++ A ++ R + LSLD + P + +++ + G + + + + + G P+ ++ +T+FR K K G IDV+WL+DDGGLTLL+P+IL TR +FA K+R+F L++R L + + +A LLLKFRI++ D+ ++SD+ K + ET F+ ++ P I+++ L+ ++T HLR+ E+ E+S S L++MTLP+PR+ + LYL+WL+ +S +PP L +RGNQ SVLT+YS
Sbjct: 97 RPTLEEL---HEARFLEKDQRRKKVEGVVEEDKSGANHKKGKVIKFGWVEGVYMRCLLNIWGVMLFLRLSWVIGQAGVGMGFAVIFLANIVTLATTLSMSAVSTNGLIEAGGIYYMISRSLGPEFGGAIGLMFTLANAVAVSMYIVGFCESVQDMLRLFGMS---IIDNS---TNDIRVVGVVTLVCILILAFVGMEWVTRTQMVLLIVLIASQIDFVVGSIMGPKTLTEMSKGFVGYDLEVFKKNLVEDY-RYFEGVEHNFFSVFSVFFPAVTGIVAGANLSGDLKDPSKAIPRGTLLAILTTFVSYLSYALMMGGCVLRDASGNVTALREALDSGLAPPALYMTISQTCLGNNSAACRYGLHNDNQAMELVSVWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIYFFSKGYGANNDPIRGYILVFCIAFGCVLIAQLNAIAPLLSNFFLAAYALINFSVFHASISKCPGWRPAFKYYNAWVSLIGTLLCIAVMFLISWWTALVTFFVVVTLYLYVSYRKPEVNWGSSTQAQSYNFALRSALELNRVQEHVKNYRPQVLALSGDPGSRPAMIDFANLITKGNSLLVCGHILKGNHSQRVHDALTQKAQRWLDQHRIKAFYAVTSERTGGTFETSAEALMQLTGLGKLKPNTVLMGFKHNW--HTCHLDELVNYFNLIHVALDSHLAVGILCLENGLDYSNYVQDKDVILISSLDKKDAEEKLRRNQSAGQLSLDGEQSDIAPAAAAVNTHPDPNGGDVTQQLDKDSESSVSESGGDLFVGPSGEPLAPNLLKDITRFRRKQDK-GFIDVWWLFDDGGLTLLVPHILRTRSQFADSKLRIFSLASRKSQLDHDQKNLAQLLLKFRIDYSDLTVISDINKPARSETRLQFENLIEKFRLRGNDPAKADGSKIITDNELVLLHEKTNRHLRLRELLFEHSLDSTLIIMTLPMPRKGTVSAPLYLSWLETLSHGLPPTLILRGNQTSVLTYYS 1082
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|939697113|ref|XP_014292708.1| (PREDICTED: bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X1 [Halyomorpha halys]) HSP 1 Score: 951.044 bits (2457), Expect = 0.000e+0 Identity = 512/1038 (49.33%), Postives = 704/1038 (67.82%), Query Frame = 0
Query: 138 LTKEVLPHVDHYRNRLSFSKGHPERS-RPTLDELLDESVLSDINGNDA-EKGV---NGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQ---YLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEG-ARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDE---------KAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVL-VTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
LT+E LP ++YR+ + P + RPTL+EL + L + D EK + A+ +GK IKFGW +GVYMRC+LNIWGVMLFLRL+WV+GQAG+ EG+ II L+N+VTV TT+SMSAVSTNG I+ GGIYYMISRSLGPEFGGAIG+MFTLANS+AVSMY++GF ESL D+LR Y+ D NG NDVRL+ +I+LV IL LA VGM+WVTR QM LL++L+ SQ+DF+IGS++ P + E AKGFVGY+ V + NL + Y E GV+H+FFSVF+VFFPAVTGIVAGANLSGDLKDP+VAIPKGTLLAI+TT+++Y+ Y L++ C +R ASGN+ + + + E L +C C YG+ Q+ME+++ WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I FS+G+GANNDP+RGY+LVF+I+ CILIG LN ++ LLSNFF+AAYALINFSVFH SI+ SPGWRP+FKYYN W+SL GT+LCI VMFL+ + TA+ T ++ LY+++ +RKP+VNWGSS+Q+Q++ AL++ L +V +HVKNYRP+I+VLSG P RP+L+DFA+L+TK SLL Q + + + DAL A W + IK+F + N + A+A + + GLGKL PNM+LMG+K+DW S + ++Y+ L A D +AVG+LR+ GLD S M D+ K ++ +RN + +LS + E + L++ + G+D S L +G+P++ + IT FR K + G ID++WLYDDGGLT+L+PHIL+ R+++++ KLRIF L+ +ELD RN+ ALLSKFRI++SD+ ++ D+TKKA +T +F+ ++ + + + D+ + EN L +T+ EL +E+TN +R+ E++ ++S ++ +V+TLP+P++ + PLYMAWL+ T MPP L++RGNQ SVLT
Sbjct: 47 LTREALPRAENYRDII-----FPAHALRPTLEELHEARFLDQVKSKDKNEKNTRRKSSAQPPKKGKIIKFGWVEGVYMRCILNIWGVMLFLRLSWVVGQAGIAEGVIIICLANLVTVATTLSMSAVSTNGIIEGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYVVGFSESLQDLLRTFGAYILD-NGT-------NDVRLVSAITLVGILVLAFVGMDWVTRTQMCLLIVLIASQLDFVIGSIVGPKTQLEEAKGFVGYNLGVFKKNLMSDYRLYE--GVEHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSVAIPKGTLLAIITTFLSYISYALIMGGCVLRDASGNITFLQTEEGIPLEERWERLT------ECNS-TCRYGLENDNQVMELVALWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPSIEFFSKGYGANNDPIRGYLLVFVIAFGCILIGQLNYIAPLLSNFFLAAYALINFSVFHVSISKSPGWRPAFKYYNAWVSLAGTLLCIAVMFLISWWTALATFFVVVTLYLYVSYRKPEVNWGSSTQAQAYYFALRSALELNKVAEHVKNYRPQILVLSGQPGARPALIDFANLITKGKSLLICTQFIKGTHSKRVHDALCDKAQHWFEQHKIKAFFVLGENAAEETNVAKALMRLSGLGKLRPNMILMGYKRDWATCSRNELDQYFAILHVALDSYMAVGILRMAEGLDYSSMIDGDDPPLSPVLERKCEIKISRNISEGQLSLDDVCSESSLSCESMKGEELSTTVPIEHTLTGKDISDL--------------YCGAEGQPLSTHHLAAITYFRRKQ-QKGFIDVWWLYDDGGLTLLIPHILSRRSQYSECKLRIFSLANRRNELDRAHRNLAALLSKFRIDYSDLKIIPDITKKAKIETIKEFEGLV------------KNFRENSSDQGE--ENGLGITDAELSLLAEKTNRHMRLRELLFEHSKDSTFIVVTLPMPRKGSVSAPLYMAWLELITAGMPPCLILRGNQQSVLT 1033 HSP 2 Score: 833.943 bits (2153), Expect = 0.000e+0 Identity = 457/994 (45.98%), Postives = 644/994 (64.79%), Query Frame = 0
Query: 1156 RPTLDELLCRSRNGSIYPCEGRS-NQSGTADCQAKDTVKKDTGPA-------RFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGN-----PEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAV-ELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQP---------KSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV---------SGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RPTL+EL E R +Q + D K+T +K + +FGW++GVY+ C+LNIWGVMLFLRL+WVVGQAG+ II +ANLVT T +SMSAV TNG I+GGGIY+MISRSLGPEFGGA+GLMFT A S+AVSMY++GF E+L D++ F + ++ NDVR++ + TLV +L ++F+GM V + Q+ LL+VL+ASQ+DF+IG+ V P + + A G VG++ + + NL D+ + +G + F VF+VFFPAVTG+VAGAN+SGDLKDP AIPKGTL AI+TT+ SY+ YAL++ +R ASGN EE + E C N C YG + Q+++L ++WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P IE F +G+G + +P+RGYLL+F IA CILIG LN ++ LLSNFF+A YA+IN+SVFH S + SPGWRP+FKY+N W+SL GT LCI VM L+ TALAT ++ LY++V R P+VNWGSS+Q+Q++ AL+S LN V +HVKNYRP+++V++G P RPAL DFANLITK SLL IK R+ D + HW + IKAF+ L N + V A+ LSGLGKL NM+++G++ W + ++Y+ +L ++ D +++VG+LR+ GLD S + + ++ + R ++ LSLD+ K +LS+ V T + ++ S++ G +G P+ + +T FR K QK G IDV+WLYDDGGLTLL+P+ILS R ++++CK+R+F L+NR + L +A+LL KFRI++ D+ I+ D+TK K ET+ F+ +V G+E G+ +++ L ++T H+R+ E+ E+S S +V+TLP+PR+ + LY+AWL+ I+ MPP L +RGNQ+SVLT+YS
Sbjct: 69 RPTLEEL-----------HEARFLDQVKSKDKNEKNTRRKSSAQPPKKGKIIKFGWVEGVYMRCILNIWGVMLFLRLSWVVGQAGIAEGVIIICLANLVTVATTLSMSAVSTNGIIEGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYVVGFSESLQDLLRTFGAYI------LDNGTNDVRLVSAITLVGILVLAFVGMDWVTRTQMCLLIVLIASQLDFVIGSIVGPKTQLEEAKGFVGYNLGVFKKNLMSDY-RLYEGVEHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSVAIPKGTLLAIITTFLSYISYALIMGGCVLRDASGNITFLQTEEGIPLEERWERLTEC-NSTCRYGLENDNQVMELVALWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPSIEFFSKGYGANNDPIRGYLLVFVIAFGCILIGQLNYIAPLLSNFFLAAYALINFSVFHVSISKSPGWRPAFKYYNAWVSLAGTLLCIAVMFLISWWTALATFFVVVTLYLYVSYRKPEVNWGSSTQAQAYYFALRSALELNKVAEHVKNYRPQILVLSGQPGARPALIDFANLITKGKSLLICTQFIKGTHSKRVHDALCDKAQHWFEQHKIKAFFVLGENAAEETNVAKALMRLSGLGKLRPNMILMGYKRDWATCSRNELDQYFAILHVALDSYMAVGILRMAEGLDYSSMIDGDDPPLSPVLERKCEIKISR-NISEGQLSLDDVCSESSLSCESMKGEELST-TVPIEHTLTGKDISDLYCGA-EGQPLSTHHLAAITYFRRKQQK-GFIDVWWLYDDGGLTLLIPHILSRRSQYSECKLRIFSLANRRNELDRAHRNLAALLSKFRIDYSDLKIIPDITKKAKIETIKEFEGLVKNFRENSSDQGEENGLG---ITDAELSLLAEKTNRHMRLRELLFEHSKDSTFIVVTLPMPRKGSVSAPLYMAWLELITAGMPPCLILRGNQQSVLTYYS 1036
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|118794513|ref|XP_321556.3| (AGAP001557-PA [Anopheles gambiae str. PEST] >gi|19572377|emb|CAD27923.1| putative Na-K-Cl symporter [Anopheles gambiae] >gi|116116330|gb|EAA00828.4| AGAP001557-PA [Anopheles gambiae str. PEST]) HSP 1 Score: 940.643 bits (2430), Expect = 0.000e+0 Identity = 484/1040 (46.54%), Postives = 694/1040 (66.73%), Query Frame = 0
Query: 132 KPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASD------EKAQMIFARNRTTSELSEQSLNFEDGS-------ESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKT--KSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
+ +H+ T+E LP +D+YRN LSF RPTLDEL D S+ N +G +G +IKFGW GV MRCLLNIWGVMLFLRL+WV+GQAG+ +G+ +I ++ +VT IT +SMSA+STNG IK GG YYMISRSLGPEFGG+IG++F+LAN++A +MY++GFCES++D+L + IVD +NDVR+IG I++VL+L + +VGMEW + Q+ LL++LLV+ VDF +GS+ P S+ ++A+GFVGY+ VL N+ +AY + G QH+FFSVF++FFPA TGI+AGAN+SGDLKDP+ +IPKGT+LAI T +Y+ ++ +R A+GNV +V +N T + CE C YG+ S Q+ME++S +GPLIYAGCFAATLSSA+ASLV AP+V QA+ KDKL+P I F +G+G NN+PVRGYIL F+IS+ IL+GDLN ++ L+SNFF+AAY L+NFS FHAS+ GWRP+FKYYN W+SLLG I CI VMFL+ + TA+IT ++ LY+ + +RKP VNWGS++Q+Q++ AL +VQ L V++HVKNYRP+I+VL G+P RP L++FA+LLTK+LSLL +T+ + K + L+ A EW + +K F+S+ ++ G+RA ++ G+GKL PN++LMGFK DW + A+ E+Y+ + A DM L+V +LR+ G D S++ D E + + A + T LS ++ GS S+ S S L+ ++ + + ++ +++ + SL G + E +E++TQF +K ++G ID+YWLYDDGGLT+LLP+I++TR +A KLR+F L+ EL+ + RNM +LL+KFRI++SD+ LL D+TKK +++ D FK G I + + K +A D A + +++ EL ++TN L + E + Q+SS++DLVVMTLP+P++ + PLYMAWL+ +R +PPFL VRGNQ SVLT
Sbjct: 127 RSFRHF-TREALPRMDNYRNILSFQGNQ----RPTLDELHDASI---TNKETMRRGTVHEPAELDG-SIKFGWIKGVLMRCLLNIWGVMLFLRLSWVVGQAGIAQGVLLICMTTVVTTITALSMSAISTNGVIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACAMYVVGFCESMIDLLASFGV---AIVDGA--VNDVRIIGCITIVLLLCIVVVGMEWEAKAQIVLLIILLVAIVDFCVGSLWGPKSELDVARGFVGYNATVLMENMQSAYRV--SKGTQHDFFSVFSIFFPAATGILAGANISGDLKDPSSSIPKGTILAIAITSASYIGMAVVAGATVLRDATGNVTDV--------------VNGTWDFSACEETSCAYGLHNSFQVMELVSVFGPLIYAGCFAATLSSALASLVSAPKVFQALCKDKLYPKISWFGKGFGKNNEPVRGYILTFVISVAVILVGDLNMIAPLISNFFLAAYCLVNFSTFHASLAKPVGWRPTFKYYNMWLSLLGAIFCIAVMFLISWPTALITFAAVLSLYLIVSYRKPDVNWGSTTQAQTYKNALLSVQQLNNVEEHVKNYRPQILVLCGHPSSRPLLVNFAYLLTKKLSLLVCGHVTKTHVSQKYRNHLQKKAAEWFRRHKVKGFYSLIDDNDFETGSRAIMQASGIGKLRPNVLLMGFKGDWDRCEPAELEQYFNVVHKALDMYLSVAILRVGKGFDYSQVLGEDTVKFISEYPRTLVANDSTNDLLSHNKVSSLHGSCDSLSRNVSQASSTSDLSKTISVAPDPIDINAKLITETGQRSLKRGDPSLLYRGPGGAELPTEVLEELTQFTSKK-KTGIIDVYWLYDDGGLTLLLPYIISTRRNWASCKLRVFALANRKTELEFEQRNMASLLAKFRIDYSDLQLLPDVTKKPNQEMADFFK----------GLIKEFTAKDDAADASTAGTST-ISKAELLAVQDKTNRHLNLREYLLQHSSKSDLVVMTLPMPRKGVVSAPLYMAWLEALSRDLPPFLFVRGNQTSVLT 1124 HSP 2 Score: 843.188 bits (2177), Expect = 0.000e+0 Identity = 456/1019 (44.75%), Postives = 639/1019 (62.71%), Query Frame = 0
Query: 1143 LMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFS-SQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERW-WLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISG------------------------AHEKVMT----------AASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSS-TRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV--------SGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
L +GNQ RPTLDEL + SI +N+ + + D G +FGWI+GV + CLLNIWGVMLFLRL+WVVGQAG+ +I + +VT++TA+SMSA+ TNG IKGGG Y+MISRSLGPEFGG++GL+F+ A ++A +MY++GFCE+++D++ F + DG V NDVRI+G T+VLLL I +GM K Q++LL++LL + VDF +G+ P E A G VG++ +L N+ ++ + G Q F VF++FFPA TG++AGAN+SGDLKDP S+IPKGT+ AI T SY+ A+V A+ +R A+GN +++ D F C +C YG + Q+++L S++GPLIYAGCFAATLSSALASLV P+VFQA+ KDKL+P I FG+GFG++ EP+RGY+L F I++ IL+GDLN ++ L+SNFF+A Y ++N+S FHAS GWRP+FKY+N W+SLLG CI VM L+ TAL T ++ LY+ V R PDVNWGS++Q+Q++ AL S+Q LN+VE+HVKNYRP+++V+ G+PS RP L +FA L+TK+ SLL HV K V + ++++ W R + +K FY+L ++ F G R ++ SG+GKL N+L++GF+ W P E+Y+ V+ + DM+LSV +LRV G D S + G +H KV + SQAS SD L+ +S+ P + +++K + G S R + + Y G + ++ +TQF K +K G IDVYWLYDDGGLTLLLPYI+STR+ +A CK+RVF L+NR L E MASLL KFRI++ D+ +L DVTK P +E F+ ++ + D +S+ LL D+T HL + E ++S S LVVMTLP+PR+ + LY+AWL+ +SR +PPFL+VRGNQ SVLTFYS
Sbjct: 147 LSFQGNQ------RPTLDEL----HDASI------TNKETMRRGTVHEPAELD-GSIKFGWIKGVLMRCLLNIWGVMLFLRLSWVVGQAGIAQGVLLICMTTVVTTITALSMSAISTNGVIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVACAMYVVGFCESMIDLLASFGVAIVDGAV-------NDVRIIGCITIVLLLCIVVVGMEWEAKAQIVLLIILLVAIVDFCVGSLWGPKSELDVARGFVGYNATVLMENMQSAYRVS-KGTQHDFFSVFSIFFPAATGILAGANISGDLKDPSSSIPKGTILAIAITSASYIGMAVVAGATVLRDATGNVTDVVNGTWD---FSACEETSCAYGLHNSFQVMELVSVFGPLIYAGCFAATLSSALASLVSAPKVFQALCKDKLYPKISWFGKGFGKNNEPVRGYILTFVISVAVILVGDLNMIAPLISNFFLAAYCLVNFSTFHASLAKPVGWRPTFKYYNMWLSLLGAIFCIAVMFLISWPTALITFAAVLSLYLIVSYRKPDVNWGSTTQAQTYKNALLSVQQLNNVEEHVKNYRPQILVLCGHPSSRPLLVNFAYLLTKKLSLLVCGHVTKTHVSQKYRNHLQKKAAEWFRRHKVKGFYSLIDDNDFETGSRAIMQASGIGKLRPNVLLMGFKGDWDRCEPAELEQYFNVVHKALDMYLSVAILRVGKGFDYSQVLGEDTVKFISEYPRTLVANDSTNDLLSHNKVSSLHGSCDSLSRNVSQASSTSD------LSKTISV---APDPIDINAKLITETGQRSLKRGDPSLLYRGPGGAELPTEVLEELTQFTSK-KKTGIIDVYWLYDDGGLTLLLPYIISTRRNWASCKLRVFALANRKTELEFEQRNMASLLAKFRIDYSDLQLLPDVTKKPNQEMADFFKGLIKEFTAKDDAADASTAGTSTISKAELLAVQDKTNRHLNLREYLLQHSSKSDLVVMTLPMPRKGVVSAPLYMAWLEALSRDLPPFLFVRGNQTSVLTFYS 1127
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|939255525|ref|XP_014247518.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Cimex lectularius]) HSP 1 Score: 935.636 bits (2417), Expect = 0.000e+0 Identity = 506/1034 (48.94%), Postives = 699/1034 (67.60%), Query Frame = 0
Query: 138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAED----SSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGN---VEEVHFGTDLFN--DTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEG--ARAALEIGGLGKLSPNMVLMGFKQDWQQS-LADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVD----SGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
LT+EVLP ++YR+ ++ SK RPTL+EL + L + + + S +GK IKFGW +GVYMRCLLNIWGVMLFLRL+WVIGQAG++EG+ +I LSN+VT+ TT+SMSAVSTNG I+ GGIYYMISRSLGPEFGGAIG+MFT+ANS+AVSMY++GFCESL D++R I+D+ ND+R++G ++LV IL LA VGM+WVTR QM LL++L+ SQ+DF+IGS++ P + E AKGFVGY+ V ++N+ Y + GV+H+FFSVF+VFFPAVTGIVAGANLSGDLKDP+VAIP+GTLLAI+TT+++Y+ Y M+ C +R ASGN +E + GT L + D + + N T C YG+ Q ME++S WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I FS+G+GANNDP+RGYILVF+I+ CILIG+LN ++ LLSNFF+AAYALINFSVFH SI+ SPGWRP+FKYYN W+SL+GT+LCI VMFL+ + TA++T ++ LY+++ +RKP+VNWGSS+Q+Q++ AL++ L +V +HVKNYRP+I+VLSG P RP+L DFA+L+TK SLL I + S +AL A W + IK+F+ + +D + E A++ + + GLGKL PNM+LMG+K DWQ + + Y+ + A D LAVG+LR+ GLD S M ++ +F E E L+ + S ++S+D +G SP K + M + G ++ ++ +T FR K ++ ID++WLYDDGGLT+L+PHI++TR++++ KLRIF L+ +ELD RN+ ALLSKFRI++SD+I++ D+TKKA +T+ +F+ +I G S+ N + + EL+ +E+TN +R+ E++ + S + V+TLP+P++ + P+YMAWL+ T MPP L++RGNQ SVLT
Sbjct: 57 LTREVLPIAENYRD-ITSSK---HALRPTLEELHEARFLDQVKAREKHDKTSRRRSFYTSSKKGKVIKFGWVEGVYMRCLLNIWGVMLFLRLSWVIGQAGIVEGIIVICLSNLVTLATTLSMSAVSTNGIIEGGGIYYMISRSLGPEFGGAIGLMFTIANSVAVSMYVVGFCESLQDLVRSL---GGTILDN--GTNDIRVVGVVTLVGILVLAFVGMDWVTRTQMCLLMVLIASQIDFVIGSIMGPKTLLEEAKGFVGYNLEVFKNNIPPNYKSFD--GVEHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSVAIPRGTLLAIITTFISYISYAFMMGGCVLREASGNETILENIEPGTSLIDRWDKISQC-NTT----------CTYGLRNDNQAMELVSLWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSKGFGANNDPIRGYILVFVIAFGCILIGNLNYIAPLLSNFFLAAYALINFSVFHVSISKSPGWRPAFKYYNAWVSLVGTLLCIAVMFLISWWTALVTFFVVVALYLYVSYRKPEVNWGSSTQAQAYYFALRSALELNKVAEHVKNYRPQILVLSGMPGHRPTLTDFANLITKGKSLLVCSHIIKGSYSRTVHEALSQKAYRWFEQHKIKAFYILGEDDGIGEHYLAKSFMTMAGLGKLKPNMILMGYKNDWQICPKQELDNYFTLIHAALDSFLAVGILRMADGLDYSNMI--EDADSPLFPSYEKKEEDEEDKLH-RNISAGQLSLDDACSAGSISPPGSPMLDQEEGIEEEENSSGKDPTEMFRGV---RGETLSTHHLQALTYFRRKQSKA-LIDVWWLYDDGGLTLLIPHIISTRSQYSDCKLRIFSLANRANELDRAHRNLAALLSKFRIDYSDLIIIPDITKKAKPETQKEFEDIIKNFRKLDGSFSK---------------NGYIADSELKLLAEKTNRHMRLRELLHEYSKDVTFYVVTLPMPRKGTVSAPMYMAWLELITAGMPPALVLRGNQTSVLT 1046 HSP 2 Score: 830.861 bits (2145), Expect = 0.000e+0 Identity = 446/988 (45.14%), Postives = 644/988 (65.18%), Query Frame = 0
Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGN--------PEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDS----FLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISD-ISGAHEKVMTAASQASGASDEDRFSMLNHA-LSLDEF-------QPKSVQLSS-KNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSG----DEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
RPTL+EL + + R T+ ++ T K +FGW++GVY+ CLLNIWGVMLFLRL+WV+GQAG++ +I ++NLVT T +SMSAV TNG I+GGGIY+MISRSLGPEFGGA+GLMFT A S+AVSMY++GFCE+L D++ G + D + ND+R++G TLV +L ++F+GM V + Q+ LL+VL+ASQ+DF+IG+ + P + A G VG++ ++ ++N+ P++ K+ DG + F VF+VFFPAVTG+VAGAN+SGDLKDP AIP+GTL AI+TT+ SY+ YA ++ +R ASGN P L+DR D++ C N CTYG + Q ++L S+WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P+I F +GFG + +P+RGY+L+F IA CILIG+LN ++ LLSNFF+A YA+IN+SVFH S + SPGWRP+FKY+N W+SL+GT LCI VM L+ TAL T ++ LY++V R P+VNWGSS+Q+Q++ AL+S LN V +HVKNYRP+++V++G P RP L DFANLITK SLL H+IK + + + + W + IKAFY L +D +L ++ + ++GLGKL NM+++G++ W + PK + Y+ ++ + D L+VG+LR+ GLD S+ I A + + + ++ ++ LSLD+ P S L + ++ SS ++ +E+ G+R G + + +T FR K K IDV+WLYDDGGLTLL+P+I+STR +++ CK+R+F L+NR + L +A+LL KFRI++ D+II+ D+TK K ET F+ ++ D + +++ L ++T H+R+ E+ E S V+TLP+PR+ + +Y+AWL+ I+ MPP L +RGNQ SVLT+YS
Sbjct: 79 RPTLEEL---HEARFLDQVKAREKHDKTSRRRSFYTSSKKGKVIKFGWVEGVYMRCLLNIWGVMLFLRLSWVIGQAGIVEGIIVICLSNLVTLATTLSMSAVSTNGIIEGGGIYYMISRSLGPEFGGAIGLMFTIANSVAVSMYVVGFCESLQDLV----RSLGGTILD--NGTNDIRVVGVVTLVGILVLAFVGMDWVTRTQMCLLMVLIASQIDFVIGSIMGPKTLLEEAKGFVGYNLEVFKNNIPPNY-KSFDGVEHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSVAIPRGTLLAIITTFISYISYAFMMGGCVLREASGNETILENIEPGTSLIDRWDKI--SQC-NTTCTYGLRNDNQAMELVSLWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSKGFGANNDPIRGYILVFVIAFGCILIGNLNYIAPLLSNFFLAAYALINFSVFHVSISKSPGWRPAFKYYNAWVSLVGTLLCIAVMFLISWWTALVTFFVVVALYLYVSYRKPEVNWGSSTQAQAYYFALRSALELNKVAEHVKNYRPQILVLSGMPGHRPTLTDFANLITKGKSLLVCSHIIKGSYSRTVHEALSQKAYRWFEQHKIKAFYILGEDDGIGEHYLA--KSFMTMAGLGKLKPNMILMGYKNDWQICPKQELDNYFTLIHAALDSFLAVGILRMADGLDYSNMIEDADSPLFPSYEKKEEDEEDKLHRNISAGQLSLDDACSAGSISPPGSPMLDQEEGIEEEENSSGKDPTEMFRGVR-GETLSTHHLQALTYFRRKQSK-ALIDVWWLYDDGGLTLLIPHIISTRSQYSDCKLRIFSLANRANELDRAHRNLAALLSKFRIDYSDLIIIPDITKKAKPETQKEFEDIIKNFRKLDGSFSKNGYIADSELKLLAEKTNRHMRLRELLHEYSKDVTFYVVTLPMPRKGTVSAPMYMAWLELITAGMPPALVLRGNQTSVLTYYS 1049
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1067092449|ref|XP_018008040.1| (PREDICTED: bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Hyalella azteca]) HSP 1 Score: 925.62 bits (2391), Expect = 0.000e+0 Identity = 505/1041 (48.51%), Postives = 680/1041 (65.32%), Query Frame = 0
Query: 132 KPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESV----LSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNV---EEVHFGTDLFN--DTVGEILNITQAYD----DCEG-RKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEE---YYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
+ L+HY+T+E LP +YRN + H ER PTLD+L D + + GND + A+ GK IKFGW GV MRCLLNIWGVMLFLR++WV+G+AGL+E L I+ N VT IT ISMSAV+TNG I GG+YYMISRSLGPEFGG+IG+MFT+ANSIAV+ YIIGF ESL DML Y ++D +ND+R +G+ L ++ LAIVGM+WVTRVQM LL LLL SQ+D IIG I P +DEE A+GFVG++ V N+ Y + G N SVFAVFF AVTGIVAGANLSGDLKDP AIPKGTLLAI T+ +Y++Y ++A +R ASGN +E ++ + N D+ T YD D E C +G+ S Q+ME+++AWGPLIY GCFAATLSSAIASLVGAPRVLQA+AKD+L+PGI FS+G+GANNDPV GY LVF+++ +CI+IG+LN VS+LLSNFF+A+Y+LINFS FHAS +RP F+YYN W+SL G +LC+ MFL+D+ TA+IT + I LY+F+ +RKP VNWGSS+QS S+++AL+A L V DHVKNYRP+I+V+SG P RP L+DFA+L+TK LS++ +++++ +D+K + N+WL K+F+++ + D L EGA ++ GLGKL PN+VL+GFK DW S+ D +E Y+ TL AF+M+L + +LR+ G D S + A DE + ++SE +N E+ ++ KK K S S A G + +E ++ +T F+ K GTID++WLYDDGGLT+LLP+ILTTR+ ++ KLRIF L+ DELD + R+M LLSKFRI++ DVI++ D +R +F+ G I + ++ D + V +TEEEL ++TN +R+ E++ +NSS A L++MTLP+P++ Q+ LYMAWLD+ ++ MPPFL VRGNQ SVLT
Sbjct: 20 RSLRHYITREALPSESNYRNIHTI---HQER--PTLDDLHDSTYKPKKADEQTGNDDVE----AKVQKRGKVIKFGWKQGVLMRCLLNIWGVMLFLRISWVVGEAGLIEALVIVCFGNTVTFITAISMSAVATNGLIAGGGVYYMISRSLGPEFGGSIGLMFTIANSIAVATYIIGFVESLQDMLFFYFDTV--MIDG--GVNDIRAVGTGVLFALMILAIVGMDWVTRVQMMLLFLLLASQLDVIIGCFIGPMTDEERARGFVGWNGEVFSDNMLREYR--DYDGEPQNIISVFAVFFTAVTGIVAGANLSGDLKDPGSAIPKGTLLAIGITFCSYILYPFLLAANVVRDASGNTTAFKEFYYTNETVNILDSPTFTECSTTGYDYVTEDNETVLACEFGLHNSFQVMELVAAWGPLIYIGCFAATLSSAIASLVGAPRVLQALAKDRLYPGIFMFSKGFGANNDPVNGYCLVFVLAFLCIMIGNLNAVSTLLSNFFLASYSLINFSCFHASFIKIGRFRPIFQYYNLWVSLFGGVLCLVTMFLIDWITALITFLVTIGLYLFVSYRKPDVNWGSSTQSASYVSALRATLTLQTVVDHVKNYRPQILVMSGRPSSRPPLLDFANLITKNLSMMVCVEVSK-DMDWKTRTVYINKCNDWLIEKKYKAFYNLVKADRLDEGAVNLFQLAGLGKLKPNVVLLGFKSDW--SVCDKQELKAYFNTLHAAFNMQLGIAILRLSNGTDYSNIIADDE-----------SPKISEGIVNEGADIETTPRAEA------------------------KKRKFSFSAAYTGPGGAKLPKEILDGVTIFKRKQ-GEGTIDVWWLYDDGGLTLLLPYILTTRSNWSNCKLRIFALANRRDELDMEQRSMANLLSKFRIDYGDVIVIPDAMSGVSDASRREFQ----------GLIEKFRTTAKCAD-----DGVSITEEELANQKDKTNRHIRLKELLLENSSTASLIIMTLPMPRKGQVSASLYMAWLDYMSKGMPPFLFVRGNQQSVLT 991 HSP 2 Score: 829.706 bits (2142), Expect = 0.000e+0 Identity = 458/1012 (45.26%), Postives = 644/1012 (63.64%), Query Frame = 0
Query: 1134 FTTRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPP-SDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNP---EELLLDRE-----DELYFQNCSNRN-------------CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKE-DVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIIL-------SDVTKHPKKETMANFQTMVSGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFY 2114
+ TR+ P N ++ RPTLD+L + S Y + Q+G D +AK V+K +FGW QGV + CLLNIWGVMLFLR++WVVG+AGL+ + I+ N VT +TAISMSAV TNG I GGG+Y+MISRSLGPEFGG++GLMFT A SIAV+ YIIGF E+L DM++ + FD ++ D ND+R +G+ L L+ ++ +GM V ++Q++LL +LLASQ+D +IG F+ P +DE+RA G VG++ ++ N+ ++ ++ DGE + VFAVFF AVTG+VAGAN+SGDLKDPGSAIPKGTL AI T+CSY++Y ++AA+ +R ASGN +E E D F CS C +G + Q+++L + WGPLIY GCFAATLSSA+ASLVG PRV QA+AKD+L+P I F +GFG + +P+ GY L+F +A CI+IG+LN VS+LLSNFF+A+Y++IN+S FHAS+ +RP F+Y+N W+SL G LC+V M L+D TAL T + I LY+FV R PDVNWGSS+QS S+++AL++ TL V DHVKNYRP+++V++G PS RP L DFANLITK S++ V V K+ D + R + K + WL + KAFY L + D G +L+GLGKL N++++GF+ W + K + Y+ L +F+M L + +LR+ G D S+I E + + +D E P++ + K SF +Y G + + I+ +T F+ K Q GTIDV+WLYDDGGLTLLLPYIL+TR ++ CK+R+F L+NR D L E MA+LL KFRI++ DVI++ SD ++ + + F+T + GV ++E L D+T H+R+ E+ ENS ++L++MTLP+PR+ ++ +LY+AWLD++S+ MPPFL+VRGNQ+SVLTFY
Sbjct: 25 YITREALPSESNYRNIHTIHQERPTLDDL-----HDSTYKPKKADEQTGNDDVEAK--VQKRGKVIKFGWKQGVLMRCLLNIWGVMLFLRISWVVGEAGLIEALVIVCFGNTVTFITAISMSAVATNGLIAGGGVYYMISRSLGPEFGGSIGLMFTIANSIAVATYIIGFVESLQDMLFFY---FDTVMIDGG--VNDIRAVGTGVLFALMILAIVGMDWVTRVQMMLLFLLLASQLDVIIGCFIGPMTDEERARGFVGWNGEVFSDNMLREY-RDYDGEPQNIISVFAVFFTAVTGIVAGANLSGDLKDPGSAIPKGTLLAIGITFCSYILYPFLLAANVVRDASGNTTAFKEFYYTNETVNILDSPTFTECSTTGYDYVTEDNETVLACEFGLHNSFQVMELVAAWGPLIYIGCFAATLSSAIASLVGAPRVLQALAKDRLYPGIFMFSKGFGANNDPVNGYCLVFVLAFLCIMIGNLNAVSTLLSNFFLASYSLINFSCFHASFIKIGRFRPIFQYYNLWVSLFGGVLCLVTMFLIDWITALITFLVTIGLYLFVSYRKPDVNWGSSTQSASYVSALRATLTLQTVVDHVKNYRPQILVMSGRPSSRPPLLDFANLITKNLSMMVCVEVSKDMDWKTRTVYINKC--NDWLIEKKYKAFYNLVKADRLDEGAVNLFQLAGLGKLKPNVVLLGFKSDWSVCDKQELKAYFNTLHAAFNMQLGIAILRLSNGTDYSNIIADDESPKISEGIVNEGADI-------------ETTPRAE--AKKRKFSF---------SAAYTGPGGAKLPKEILDGVTIFKRK-QGEGTIDVWWLYDDGGLTLLLPYILTTRSNWSNCKLRIFALANRRDELDMEQRSMANLLSKFRIDYGDVIVIPDAMSGVSDASRREFQGLIEKFRTTAKCADDGVS---ITEEELANQKDKTNRHIRLKELLLENSSTASLIIMTLPMPRKGQVSASLYMAWLDYMSKGMPPFLFVRGNQQSVLTFY 993 The following BLAST results are available for this feature:
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 5
BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold1253_size52701:29378..36696- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold1253_size52701-snap-gene-0.12 ID=maker-scaffold1253_size52701-snap-gene-0.12|Name=af190129_1na+ k+ 2cl- cotransporter|organism=Tigriopus kingsejongensis|type=gene|length=7319bp|location=Sequence derived from alignment at scaffold1253_size52701:29378..36696- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'af190129_1na+ k+ 2cl- cotransporter' has the following synonyms
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