BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000003059 (supercontig:LSalAtl2s:LSalAtl2s1734:14347:19068:1 gene:EMLSAG00000003059 transcript:EMLSAT00000003059 description:"maker-LSalAtl2s1734-augustus-gene-0.5")

HSP 1 Score: 1278.46 bits (3307), Expect = 0.000e+0
Identity = 672/1127 (59.63%), Postives = 851/1127 (75.51%), Query Frame = 0

Query:   66 GECPSGSDPSFPD---VPECLTPSPYLP---TRNGN--------RKRSSVTIA-PGTILEKPLPSQRKMTSKQRSSFILTPKPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVER-INDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGL----ASPATLTKRKLGRDGSVLGR--------------FQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNK-SIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMI---TIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            G  PSG D +F +   + + ++   YL     R+ N        R+R SVTI+ P    EKP PS R+++ K+ S    T + L+HYLT+E +PH DHYRN++SF+KG P+ SRPTLD+LL++   S  N +D E   +  +D   G  IKFGW +GVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGL IITLSNIVTVIT+ISMSAVSTNGQIKAGGIY+MISRSLGPEFGGAIG+MFTLANSIAV+MYIIGFCE+L DML+QY+  F GI+++ E  ++D+RLIGS+SLVL+L LAIVGMEWVTRVQ GLL+LLL SQ+DFIIGS +P S++++AKGFVGY+  VL  NL++ Y  +E +   ++FF+VFAVFFPAVTGIVAGANLSGDLKDP VAIPKGTLL IV+TYVTY IYG+M+A  + R ASG+V E+    D  N+T+ E+ NIT ++ +CE R C+YGIL SQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRV QAVAKDKLFPGI SF++GWG NNDPV+GY+LVF+ISLICILIGDLN VSSLLSNFFVAAYALINFSVFHASIT SPGWRP FKYYNKWISLLGT+LCI VMFLMD  TA++T + +  LY+++  RKP+VNWGSS+Q+QSF+TAL +V +LT+V+DHVKNYRPKIMV+SG+P++RP+L+DFA+L+TKR+SLLES+ +    LD+KK  A+K++   W   NHIK F S+ RN  + EGAR A+E+ GLGKL  NM+L+GF+  W +    A+EYY+ ++TAFD+RLAVGVLRI+GGLD++ M    E           +S     +LN++D  ES MSVDSGL     S A  T  + G D    G               F+KK  SS+ L   +KD +P++E+ V +++QFR+K ++ +GTID+YWLYDDG       HIL TR K++K K+R+FFLS   DELD  TR+M +LL+KFRI+F DVI+L D TK+  K+T+D+FKAM+   ++ +  SG+   +S +  +  + D +   LV+E ++  + E+TNF LRIAEIV+ NSS ADL+VMTLP+PK+E +P  LYMAWLD+TT+ MPPFL+VRGNQ SVLT
Sbjct:   27 GGDPSGGDDNFSENGSIDDSISYKNYLAYELNRSSNPGGGGGGDRRRPSVTISVPEN--EKPSPSSRRLSRKRSSFQFTTQRSLRHYLTREAIPHADHYRNKMSFAKGKPDSSRPTLDDLLNDDPYSKDN-SDLE---SQTDDQDTGSVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLAIITLSNIVTVITSISMSAVSTNGQIKAGGIYFMISRSLGPEFGGAIGLMFTLANSIAVAMYIIGFCEALSDMLKQYIPGFIGILNTPEDGLHDIRLIGSVSLVLLLGLAIVGMEWVTRVQKGLLVLLLFSQLDFIIGSFLPPSEDKLAKGFVGYNGTVLMENLFSEYIPNEGTEKNNSFFTVFAVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLXIVSTYVTYTIYGVMIAAXTXREASGDVLEL----DYVNETIKEVNNITNSFWECENRTCSYGILHSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVFQAVAKDKLFPGIASFADGWGKNNDPVKGYLLVFLISLICILIGDLNYVSSLLSNFFVAAYALINFSVFHASITKSPGWRPGFKYYNKWISLLGTVLCICVMFLMDMVTALVTFIIVFFLYMYVSIRKPEVNWGSSTQAQSFMTALNSVLSLTKVEDHVKNYRPKIMVMSGSPLNRPTLIDFANLVTKRISLLESVDVVPEDLDWKKSQAIKNSGQNWFTENHIKGFFSVVRNSKISEGARTAMELSGLGKLRTNMILLGFQDTWWKKDESAKEYYKIIQTAFDLRLAVGVLRINGGLDVTSMTNLMED----------SSPNQSNALNYDDRCESNMSVDSGLDLATKSGAKNTSTQSGEDDKEDGSLKRNKKPNGFEKLLFKKKCPSSIPLPPSEKDVKPLDEDIVNRLSQFRDKTTVNTGTIDVYWLYDDGXXXX---HILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVP-PLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLIVMTLPMPKKEIVPQELYMAWLDYTTKDMPPFLLVRGNQTSVLT 1129          

HSP 2 Score: 1021.92 bits (2641), Expect = 0.000e+0
Identity = 548/1053 (52.04%), Postives = 717/1053 (68.09%), Query Frame = 0

Query: 1120 REQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPA-RFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLR-LFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELY---------FQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNV-------DSFGTSSTRNNSEVSY-----------------GIRDGNPIDERIVYRMTQFRDKSQ-KVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGD---------------------EQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            RE IP+       D    KM      +G  DS   SRPTLD+LL    N   Y  +    +S T D        +DTG   +FGWI+GVY+ CLLNIWGVMLFLRLTWV+GQAGLL   AIIT++N+VT +T+ISMSAV TNGQIK GGIYFMISRSLGPEFGGA+GLMFT A SIAV+MYIIGFCEAL DM+ Q+   F GI+   E   +D+R++GS +LVLLLG++ +GM  V ++Q  LL++LL SQ+DF+IG+F+PPS++K A G VG++  +L  NL+ ++  N   E+    F VFAVFFPAVTG+VAGAN+SGDLKDPG AIPKGTL  I++TY +Y +Y ++IAA   R ASG+  EL  D  +E           F  C NR C+YG L +QQM+++ S WGPLIYAGCFAATLSSA+ASLVG PRVFQA+AKDKLFP I SF  G+G++ +P++GYLL+F I+L CILIGDLN VSSLLSNFFVA YA+IN+SVFHAS   SPGWRP FKY+NKWISLLGT LCI VM LMD+ TAL T I +  LY++V  R P+VNWGSS+Q+QSF+TAL S+ +L  VEDHVKNYRPK++V++G+P +RP L DFANL+TKR SLLE V V+ ED++ +    +K    +W  +NHIK F+++ RN     G RTA+ELSGLGKL  NM+++GFQ+ WW   +  +EYY+++  +FD+ L+VGVLR+ GGLD++ ++   E   ++ +Q++  + +DR       +S+D     + +  +KN        D    S  RN     +                   +D  P+DE IV R++QFRDK+    GTIDVYWLYDDG       +IL TR K++KCKMRVFFLSN+ D L  +   MASLL KFRI+F+DVI+L+D TK PKKET   F+ M++ +                     E     P+VSE++++ + ++T F+LRIAEI K+NS  + L+VMTLP+P++  +P  LY+AWLD+ ++ MPPFL VRGNQ SVLTFYS
Sbjct:  114 REAIPHA------DHYRNKMS---FAKGKPDS---SRPTLDDLL----NDDPYSKDNSDLESQTDD--------QDTGSVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLAIITLSNIVTVITSISMSAVSTNGQIKAGGIYFMISRSLGPEFGGAIGLMFTLANSIAVAMYIIGFCEALSDMLKQYIPGFIGILNTPEDGLHDIRLIGSVSLVLLLGLAIVGMEWVTRVQKGLLVLLLFSQLDFIIGSFLPPSEDKLAKGFVGYNGTVLMENLFSEYIPNEGTEKNNSFFTVFAVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLXIVSTYVTYTIYGVMIAAXTXREASGDVLEL--DYVNETIKEVNNITNSFWECENRTCSYGILHSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVFQAVAKDKLFPGIASFADGWGKNNDPVKGYLLVFLISLICILIGDLNYVSSLLSNFFVAAYALINFSVFHASITKSPGWRPGFKYYNKWISLLGTVLCICVMFLMDMVTALVTFIIVFFLYMYVSIRKPEVNWGSSTQAQSFMTALNSVLSLTKVEDHVKNYRPKIMVMSGSPLNRPTLIDFANLVTKRISLLESVDVVPEDLDWKKSQAIKNSGQNWFTENHIKGFFSVVRNSKISEGARTAMELSGLGKLRTNMILLGFQDTWWKKDESAKEYYKIIQTAFDLRLAVGVLRINGGLDVTSMTNLMED--SSPNQSNALNYDDR---CESNMSVDSGLDLATKSGAKNTSTQSGEDDKEDGSLKRNKKPNGFEKLLFKKKCPSSIPLPPSEKDVKPLDEDIVNRLSQFRDKTTVNTGTIDVYWLYDDGXXXX---HILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVPPLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLIVMTLPMPKKEIVPQELYMAWLDYTTKDMPPFLLVRGNQTSVLTFYS 1132          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000009267 (supercontig:LSalAtl2s:LSalAtl2s58:680408:685072:1 gene:EMLSAG00000009267 transcript:EMLSAT00000009267 description:"augustus_masked-LSalAtl2s58-processed-gene-6.1")

HSP 1 Score: 514.998 bits (1325), Expect = 5.586e-159
Identity = 336/695 (48.35%), Postives = 429/695 (61.73%), Query Frame = 0

Query:  113 LPSQRKMTSKQRSSFILTP-----KPLKHYLTKEVLPHVDHYRNRLSFSKGHPER-SRPTL----------DELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRK----CNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQ----VSSLLSNFFVAAYALI-----------NFSVFHASITSSPGWRPSF-KYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKV-NWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVL--SGNPVDRPSLMD-FAHLLTKRLSLLESIQI--TEASLDFKKV 765
            +P   K   ++ +SF  T      K  + YLT++ LP   HYRN LS S  HP + SRPTL            LLD+  + D+  N+        E+ S+GK ++FGW +GV+MRCLLNIWGVMLFLRLTWV+GQ GL++GLG+I L N+VTVITT+SMSAVSTNGQI+ GGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFC+SL+DM +QY  +   ++ S +  NDVR+IG+ISLV +LALAIVGMEWVTRVQM LL LL+ SQ+DF++GS +P+  E+ A GFVGY+  +   NL   Y   +  G   +FFSVF VFFPAVTGIVAGANLSGDLKDP VAIPKGTLLAI+ T++TY+ YG ++       ASGN  E     +  N   G +      +++C GR     C YG    QQ ME IS  G LIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+P IH F+ G+GANNDP+RGY LVFIIS+ CILI   +Q    V  L    FV     I           N+       T  P  RP    + N     L  ++C  V    +   A    + ++   V ++FR  K+ ++ S +Q+++F     A   L      +   RP +++    G+ ++ P  +D +  +L     L   + I   E+  DF KV
Sbjct:   36 VPDHDKAGGEETNSFHPTYSTQYFKSFRQYLTRDALPSESHYRNLLSISN-HPRKFSRPTLEELQEEQAENGRLLDKKDMDDVEKNE--------EEQSKGKVVRFGWIEGVFMRCLLNIWGVMLFLRLTWVVGQGGLIQGLGVIALGNLVTVITTLSMSAVSTNGQIRGGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCQSLLDMCKQYF-ELEHLIFS-DTTNDVRIIGAISLVAVLALAIVGMEWVTRVQMILLFLLIGSQIDFVVGSFLPTK-EDAAYGFVGYNGTLFVDNLSPTYHDYKDPGNPPSFFSVFGVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLAIIVTFITYIGYGTIITGVMHTEASGNQTEYE---NWINGVEGALY-----FNNCSGRPADDFCQYGSSNDQQAMERISYTGYLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIHIFAPGFGANNDPIRGYFLVFIISMGCILIATESQRLTGVVQLKXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHV----NIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLA----GIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKV 702          

HSP 2 Score: 419.853 bits (1078), Expect = 1.735e-125
Identity = 238/463 (51.40%), Postives = 304/463 (65.66%), Query Frame = 0

Query: 1185 DCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVD-GEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEEL---LLDREDELYFQNCSNRN----CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGD----LNRVSSLLSNFFVATYAVINYSVFHASWNNSP----GWRPSFKYF 1631
            D +  +  +      RFGWI+GV++ CLLNIWGVMLFLRLTWVVGQ GL+    +I + NLVT +T +SMSAV TNGQI+GGGIY+MISRSLGPEFGGA+G+MFT A SIAVSMYIIGFC++LLDM  Q+      I  D+    NDVRI+G+ +LV +L ++ +GM  V ++Q++LL +L+ SQ+DF++G+F+ P+ E  A G VG++  L   NL P +    D G     F VF VFFPAVTG+VAGAN+SGDLKDPG AIPKGTL AI+ T+ +Y+ Y  +I       ASGN  E    +   E  LYF NCS R     C YG+  +QQ ++  S  G LIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P+I  F  GFG + +P+RGY L+F I++ CILI      L  V  L    FV T   I       S N++P     +RP    F
Sbjct:  116 DVEKNEEEQSKGKVVRFGWIEGVFMRCLLNIWGVMLFLRLTWVVGQGGLIQGLGVIALGNLVTVITTLSMSAVSTNGQIRGGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCQSLLDMCKQYFELEHLIFSDT---TNDVRIIGAISLVAVLALAIVGMEWVTRVQMILLFLLIGSQIDFVVGSFL-PTKEDAAYGFVGYNGTLFVDNLSPTYHDYKDPGNPPSFFSVFGVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLAIIVTFITYIGYGTIITGVMHTEASGNQTEYENWINGVEGALYFNNCSGRPADDFCQYGSSNDQQAMERISYTGYLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIHIFAPGFGANNDPIRGYFLVFIISMGCILIATESQRLTGVVQLKXQQFVTTLKSIQ------SLNDTPEHVKNYRPKVLVF 568          

HSP 3 Score: 345.895 bits (886), Expect = 2.637e-100
Identity = 185/433 (42.73%), Postives = 263/433 (60.74%), Query Frame = 0

Query: 1683 QSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            + Q F+T LKS+Q+LN   +HVKNYRPK++V TG P+ RP L DFANLITK+ SLL   HV   D   + ++ ++  V  W RD+ IK+FY+LT+N +F  G    + L+G+GK+  NM++ G++  W   PK  +EY  VL  +FD+HL VG+LR++ G D S + G  E+            DE               Q KS++L  K V        R  S   Y   DGNP+ +  V  +TQF+ K +K G IDV+WLYDDGGLTLLLPYIL+TR +FA+C++RVF L+NR D L  E   MA LL KFRI +  V ++ DVTK     T   F+ ++  DE    +P + E  ++   ++T  HLR+ E+ +E+S  S +++MTL +PR+      LY++WL+ ++++MPPFL +RGNQ SVLTFYS
Sbjct:  537 KXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHVNIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLAGIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKVIGEEEQQQHEIELDEENEDEKNGK-----------QGKSLELQQKKV------RXRKVSTAVYTGIDGNPLPKNTVQDITQFQIKKRK-GNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKIL--DE---IKPPLEESEIISQKEKTNRHLRLTELLREHSKRSEMIIMTLSIPRRGTAVAQLYMSWLEMMTKNMPPFLLIRGNQSSVLTFYS 946          

HSP 4 Score: 337.421 bits (864), Expect = 2.045e-97
Identity = 183/462 (39.61%), Postives = 271/462 (58.66%), Query Frame = 0

Query:  695 QSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKS-SMSLAILDK-DGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            + Q F+T LK++Q+L    +HVKNYRPK++V +G P  RP L+DFA+L+TK+LSLL    +      FK ++ L++    W   + IKSF+S+T+N +  EGA A + + G+GK+ PNMVL G+K DW +     +EY   L  AFD+ L VG+LR++ G D S++   +E+ Q          EL E++   ED                     K G+ G  L   QKK +   +S A+    DG P+ + TV+ ITQF+ K  R G ID++WLYDDGGLT+LLP+ILTTR +FA+ +LR+F L+   DELD +TRNM  LL+KFRI +  V ++ D+TKKA   T+++FK ++    P                         + E E+    E+TN  LR+ E++R++S  +++++MTL +P+R      LYM+WL+  T+ MPPFL++RGNQ SVLT
Sbjct:  537 KXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHVNIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLAGIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKVIGEEEQQQ-------HEIELDEEN---ED--------------------EKNGKQGKSLELQQKKVRXRKVSTAVYTGIDGNPLPKNTVQDITQFQIKK-RKGNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKILDEIKPP------------------------LEESEIISQKEKTNRHLRLTELLREHSKRSEMIIMTLSIPRRGTAVAQLYMSWLEMMTKNMPPFLLIRGNQSSVLT 943          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000003697 (supercontig:LSalAtl2s:LSalAtl2s1:1949199:1954526:1 gene:EMLSAG00000003697 transcript:EMLSAT00000003697 description:"maker-LSalAtl2s1-augustus-gene-19.17")

HSP 1 Score: 278.1 bits (710), Expect = 2.704e-78
Identity = 200/665 (30.08%), Postives = 337/665 (50.68%), Query Frame = 0

Query:  191 AEDSSEGKAIK-FGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMD-------MLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSS-----DEEIAKGFVG-----YDTAVLESNLYNAYT---KDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCE--GRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMD-FKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLN-SNH--IKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLAD 828
            AED   G+     GWF G +    L+++  +LFLR+ +++G AG+L  L  I ++  + V T +S+SA++TNG +K GG+Y+MISR+LGPEFGG+IG++F  AN  + ++Y +   E ++         L  YL + +     + R+    LI     +L L++ ++G     +  + +L+ ++   +  I      S+     +E I+  F G     +     +S+LY +Y+    D+T  V  NF  VFAV F  VTGI+AGANLSGDLK+P+ +IPKGTLLA   T++TY+I                                 +  +T    D E   R C+Y       M+E  S W P +  G   AT  ++ + L+G+ RVL+A++KD +F     +       N+PV   ++ +++S I   IG +N ++  ++  F+ +YA +N S     + S+P +RP F+ ++ +   LG I  + +MF+++   +A++   CI       +    K NWGS SQ+  F    K +  L    DHVK +RP++++L  NP +  SL+DFA+ L K+  L     + E + +  + D        WLN  +H  +K+F  +T   ++  G +  + I G+G + PN ++ GF  + +    D
Sbjct:   11 AEDEHVGRGSGGLGWFKGXFAPVSLSMFSSLLFLRVGYILGNAGILHSLLQIVIAYGILVFTILSVSAIATNGAVKGGGVYFMISRTLGPEFGGSIGVLFYAANIASSALYAVACTECIISSFGEESGTLAHYLPE-SFWYQILYRV----LIN----LLNLSICLIGPNLFGKTTIMVLISVVFCTLTVITSFFSASTVEVHFNETISGSFTGILSKNWSIENFQSHLYPSYSLDCNDDTKPV--NFAIVFAVLFSGVTGIMAGANLSGDLKEPSKSIPKGTLLASFFTFITYVI---------------------------------LFTLTAMTCDKELLLRDCSY-------MIE-FSFWKPFVIIGTVLATFCASTSCLIGSSRVLEAISKDTMFGIFLEWITYGTVGNNPVLAVVVTWLLSNIFFFIGGVNTIAQFVAVLFLLSYASVNLSCLSLDLASAPNFRPIFRCFSWYTCALGLIGTLTMMFMINPVISALVLFACIFFFLSLSFLSPTKENWGSISQALLFHQVRKYLLLLDSRKDHVKFWRPQVLLLVSNPRNHCSLIDFANAL-KKGGLYVLGHVREGNFEGMEEDECGLLMPHWLNLVDHLKVKAFVELTVAPTVRSGIQQLIRISGIGAMKPNTIIFGFPYEDENMCKD 622          

HSP 2 Score: 271.937 bits (694), Expect = 3.037e-76
Identity = 189/627 (30.14%), Postives = 320/627 (51.04%), Query Frame = 0

Query: 1197 GPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKR--------AMGIV--GFDPDLLRSNLWPDFQ--KNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFP-YIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMD-LGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLR-DNH--IKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGF 1806
            G    GW +G + P  L+++  +LFLR+ +++G AG+L S   I IA  +   T +S+SA+ TNG +KGGG+YFMISR+LGPEFGG++G++F  A   + ++Y +   E ++    + S      + +S       R+L +   +L L I  IG  L  K  +++L+ ++   +  +   F   + E            GI+   +  +  +S+L+P +    N D + +    VFAV F  VTG++AGAN+SGDLK+P  +IPKGTL A   T+ +Y++   + A +         +ELLL             R+C+Y       MI+  S W P +  G   AT  ++ + L+G  RV +AI+KD +F  ++E    G      P+   ++ + ++     IG +N ++  ++  F+ +YA +N S       ++P +RP F+ F+ +   LG    + +M +++ + +AL    CI              NWGS SQ+  F    K L  L+  +DHVK +RP+++++  NP +  +L DFAN + K+  L  L HV + + E    D     + HWL   +H  +KAF  LT   +   G++  + +SG+G +  N ++ GF
Sbjct:   19 GSGGLGWFKGXFAPVSLSMFSSLLFLRVGYILGNAGILHSLLQIVIAYGILVFTILSVSAIATNGAVKGGGVYFMISRTLGPEFGGSIGVLFYAANIASSALYAVACTECIISSFGEESGTLAHYLPESFWYQILYRVLIN---LLNLSICLIGPNLFGKTTIMVLISVVFCTLTVITSFFSASTVEVHFNETISGSFTGILSKNWSIENFQSHLYPSYSLDCNDDTKPVNFAIVFAVLFSGVTGIMAGANLSGDLKEPSKSIPKGTLLASFFTFITYVILFTLTAMTC-------DKELLL-------------RDCSY-------MIEF-SFWKPFVIIGTVLATFCASTSCLIGSSRVLEAISKDTMFGIFLEWITYG-TVGNNPVLAVVVTWLLSNIFFFIGGVNTIAQFVAVLFLLSYASVNLSCLSLDLASAPNFRPIFRCFSWYTCALGLIGTLTMMFMINPVISALVLFACIFFFLSLSFLSPTKENWGSISQALLFHQVRKYLLLLDSRKDHVKFWRPQVLLLVSNPRNHCSLIDFANAL-KKGGLYVLGHVREGNFEGMEEDECGLLMPHWLNLVDHLKVKAFVELTVAPTVRSGIQQLIRISGIGAMKPNTIIFGF 612          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000004234 (supercontig:LSalAtl2s:LSalAtl2s222:1210791:1213007:1 gene:EMLSAG00000004234 transcript:EMLSAT00000004234 description:"maker-LSalAtl2s222-snap-gene-12.7")

HSP 1 Score: 217.624 bits (553), Expect = 6.899e-60
Identity = 172/614 (28.01%), Postives = 290/614 (47.23%), Query Frame = 0

Query:  256 SAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISL-VLILALAIVGMEWVTRVQMGLLLLLLVSQV--------------DFIIGSVIPSSDEEIAK------------------GFVGYDTAVLESNLY-NAYTKDE--TSGVQH--NFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQM-MEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKP-----KVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKR-LSLLESIQITEASLDFKKVDALKSAANEWL---NSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQD 821
            SA+S+NG ++ GG+Y+M+SR+LGPEFGGAIG +F  AN ++ ++Y+ G  E L+   +            + R   +  + S S+  L L + +VG ++  +  + +  ++LV  +              DFI  +  P    + +K                  G   ++  +L  N Y N Y K E   SG     NFF +F V F  VTGI++GAN+ G+L  P+ +IP+GT+ A   T V Y+I  ++ +                                          C+  +LQ   M M +++   P +  G F AT S+++ +L+G  R+L+A+AKD +F  + +F        +PV   I  + +  + +++G LN ++ L S  F+ +Y  IN +     + S+P +RP++KY++ +  L+G +  I +MF +   +AI + V I+L    I            NWGS SQ+  F    K +  L +   HVK +RP++++L  NP    SL++F + L K  L +L  + +   +L+    D   +    WL   +   +K+F  +T   SL  G    + I G+G + PN VL+GFK D
Sbjct:   16 SALSSNGAVEGGGVYFMLSRTLGPEFGGAIGTLFFFANVVSSALYLAGCTEGLVSNFK-----------FLARGPWIEFLYSTSINSLNLFICLVGSKFFGKTSVLIFFMVLVCVLMTSVSFFLDYTITEDFIFNATDPLKCVQPSKNDTNQTPNCTLTSTGKFTGIGSWNETILVQNFYDNLYPKYEYDCSGSDKSLNFFIIFGVLFSGVTGIMSGANMCGELVSPSKSIPRGTISACGFTLVIYIILTVLTSL----------------------------------------TCSPTLLQHDCMYMNIVNVLPPAVTIGVFMATFSASLNNLIGXSRILEAIAKDVVFGPLFNFVLKGTYKENPVTAVISTWFVVELFLMMGSLNNIAQLCSVLFLLSYGTINLTCLFLHLASAPNFRPTYKYFSTYTCLIGLLGSIIMMFAV---SAIYSAVGILLCLSLILLLNLFSPIRHTNWGSISQALIFHQVRKYLLLLDQRKSHVKFWRPQMLLLVSNPRRNCSLIEFGNALKKSGLYVLGHVHVD--NLEEHDKDPTLNEIPHWLKLIDFLKVKAFVEVTIARSLRLGVDQLVRISGIGAMKPNTVLLGFKDD 573          

HSP 2 Score: 214.927 bits (546), Expect = 5.054e-59
Identity = 163/592 (27.53%), Postives = 287/592 (48.48%), Query Frame = 0

Query: 1255 SAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTF--------------VPPSDEK-------------RAMGIVGFDPDLL----RSNLWPDFQKNVDG--EQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNP--DVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLR---DNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQE 1808
            SA+ +NG ++GGG+YFM+SR+LGPEFGGA+G +F  A  ++ ++Y+ G  E L+   ++F ++   I        N + +      + L+G  F G   V    ++L+ VL+ S   FL  T               V PS                +  GI  ++  +L      NL+P ++ +  G  + L  F +F V F  VTG+++GANM G+L  P  +IP+GT++A     C + +  + I  + +   + +P  L                +C Y  + N        +  P +  G F AT S++L +L+G  R+ +AIAKD +F  + +F       + P+   +  + +    +++G LN ++ L S  F+ +Y  IN +       ++P +RP++KYF+ +  L+G    I++M  +    +   ++  + L + +   +P    NWGS SQ+  F    K L  L+  + HVK +RP+++++  NP    +L +F N + K+S L  L HV  +++E    D     + HWL+      +KAF  +T   S   GV   V +SG+G +  N +++GF++
Sbjct:   16 SALSSNGAVEGGGVYFMLSRTLGPEFGGAIGTLFFFANVVSSALYLAGCTEGLVSN-FKFLARGPWIEFLYSTSINSLNLF-----ICLVGSKFFGKTSVLIFFMVLVCVLMTSVSFFLDYTITEDFIFNATDPLKCVQPSKNDTNQTPNCTLTSTGKFTGIGSWNETILVQNFYDNLYPKYEYDCSGSDKSLNFFIIFGVLFSGVTGIMSGANMCGELVSPSKSIPRGTISA-----CGFTL-VIYIILTVLTSLTCSPTLL--------------QHDCMYMNIVN--------VLPPAVTIGVFMATFSASLNNLIGXSRILEAIAKDVVFGPLFNFVLKGTYKENPVTAVISTWFVVELFLMMGSLNNIAQLCSVLFLLSYGTINLTCLFLHLASAPNFRPTYKYFSTYTCLIGLLGSIIMMFAVSAIYSAVGILLCLSLILLLNLFSPIRHTNWGSISQALIFHQVRKYLLLLDQRKSHVKFWRPQMLLLVSNPRRNCSLIEFGNAL-KKSGLYVLGHVHVDNLEEHDKDPTLNEIPHWLKLIDFLKVKAFVEVTIARSLRLGVDQLVRISGIGAMKPNTVLLGFKD 572          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000007778 (supercontig:LSalAtl2s:LSalAtl2s455:327857:377250:-1 gene:EMLSAG00000007778 transcript:EMLSAT00000007778 description:"maker-LSalAtl2s455-augustus-gene-2.20")

HSP 1 Score: 186.037 bits (471), Expect = 1.310e-47
Identity = 119/432 (27.55%), Postives = 208/432 (48.15%), Query Frame = 0

Query:  410 TSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWG--PLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVL----SGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLD--FKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYY 833
            TS +  +F  +  +FFP+ TGI+AG+N SGDL  P   IP GT++AI+TT   Y+   L+ A          V+ +    D F +++G  L +                           AW    ++  G   +T+ + + SL GAPR+LQA+AKD + P +  F +   A  +PVR   +  +I  + +LIG++++++ LLS FF+  Y  +N +    ++  +P WRP FKYY+  +SLLG +LCI VMF+  +  A++      ++Y +I +R  +  WG   +  +   A  ++  L     H KN+RP+I++L    S   V    L   A  L     L   + + +      + +  A K +  + +    +K F  +  +  + +G     +  GLG + PN V++G+   W++   D+ + +
Sbjct:  442 TSDIYTSFVILVGIFFPSCTGIMAGSNRSGDLAXPQKXIPIGTIMAILTTSTVYLSSVLLFA--------ATVDPLLL-RDKFGESIGGSLVVGNI------------------------AWPHPKVVEIGSLLSTIGAGLQSLTGAPRLLQAIAKDGIIPFLAPF-QVQSAKGEPVRALGITLLICEVAVLIGNVDEIAPLLSMFFLMCYGFVNLAXALQTLLRTPNWRPRFKYYHWSLSLLGVVLCIAVMFMSSWYYALLAMGIAGIIYKYIEYRGAEKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILAKLDSQLKVKHKRLFALASQLKAGKGLTIGVTVLDGHFGKMYGEAQAAKFSIQQVIKDEAVKGFADVVVSKDISDGVCHLTQTTGLGGMKPNTVILGWPNSWRKKEEDSWKVF 839          

HSP 2 Score: 162.54 bits (410), Expect = 3.122e-40
Identity = 116/424 (27.36%), Postives = 192/424 (45.28%), Query Frame = 0

Query: 1421 AVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWG--PLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKED----VEPRIMDN-----------MKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLS 1827
             +FFP+ TG++AG+N SGDL  P   IP GT+ AILTT   Y+   L+ AA+   +       LL D+               +G      ++  +  W    ++  G   +T+ + L SL G PR+ QAIAKD + P++  F     +  EP+R   +   I    +LIG+++ ++ LLS FF+  Y  +N +    +   +P WRP FKY++  +SLLG  LCI VM +     AL  +    ++Y ++  R  +  WG   +  +   A  SL  L     H KN+RP+++++    S          L  K   L  L   +K      +   ++D             K  +   ++D  +K F  +  +     GV    + +GLG +  N +++G+   W    K  E+ ++V V S
Sbjct:  454 GIFFPSCTGIMAGSNRSGDLAXPQKXIPIGTIMAILTTSTVYLSSVLLFAATVDPL-------LLRDK---------------FGESIGGSLVVGNIAWPHPKVVEIGSLLSTIGAGLQSLTGAPRLLQAIAKDGIIPFLAPFQVQSAKG-EPVRALGITLLICEVAVLIGNVDEIAPLLSMFFLMCYGFVNLAXALQTLLRTPNWRPRFKYYHWSLSLLGVVLCIAVMFMSSWYYALLAMGIAGIIYKYIEYRGAEKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILAKLDS---------QLKVKHKRLFALASQLKAGKGLTIGVTVLDGHFGKMYGEAQAAKFSIQQVIKDEAVKGFADVVVSKDISDGVCHLTQTTGLGGMKPNTVILGWPNSW---RKKEEDSWKVFVDS 842          

HSP 3 Score: 139.428 bits (350), Expect = 3.895e-33
Identity = 84/202 (41.58%), Postives = 119/202 (58.91%), Query Frame = 0

Query: 1178 SNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFS-SQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV 1378
            +N S T      D   K    A  G + GVY+PC+ NI+G +LF+R+TW+VG AG +  F ++ +   VT +TAISM+ + TNG + GGG  FMISR+LGPEFGGAVG+ F  A  +A +MYIIG  E L+  I   + S F   + +   + N+ R+ G+  L+ +  I FIG+  VNK   + L  +L S V   +G FV
Sbjct:  124 ANYSATIQSXPTDPDAKPVQSANLGTLMGVYLPCIQNIFGXILFIRMTWIVGTAGAVEGFFVVFMGCCVTLLTAISMAXIATNGVVPGGGSXFMISRALGPEFGGAVGIXFYXAXXVAAAMYIIGAIEILIIYIVGPAMSLFGPDITNDFVKFNNYRVYGTFLLITMGTIVFIGVRFVNKFAAVALACVLLSIVSIYVGIFV 325          

HSP 4 Score: 119.398 bits (298), Expect = 5.810e-27
Identity = 67/185 (36.22%), Postives = 110/185 (59.46%), Query Frame = 0

Query:  193 DSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNG--IVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIG 375
            D+   ++   G   GVY+ C+ NI+G +LF+R+TW++G AG +EG  ++ +   VT++T ISM+ ++TNG +  GG  +MISR+LGPEFGGA+GI F  A  +A +MYIIG  E L+  +        G  I +   + N+ R+ G+  L+ +  +  +G+ +V +     L  +L+S V   +G
Sbjct:  138 DAKPVQSANLGTLMGVYLPCIQNIFGXILFIRMTWIVGTAGAVEGFFVVFMGCCVTLLTAISMAXIATNGVVPGGGSXFMISRALGPEFGGAVGIXFYXAXXVAAAMYIIGAIEILIIYIVGPAMSLFGPDITNDFVKFNNYRVYGTFLLITMGTIVFIGVRFVNKFAAVALACVLLSIVSIYVG 322          

HSP 5 Score: 54.6842 bits (130), Expect = 2.949e-7
Identity = 47/205 (22.93%), Postives = 106/205 (51.71%), Query Frame = 0

Query:  961 GTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFS-DVILLQ--DMTKKADKKT-----RDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPK---REQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            G IDI+W+  DGGL MLLP +L     +   KLRIF ++++ D   +  +++   L   RIE   +V+ +Q  D+++   ++T     R++    + +   + G++++    ++ V ++  ++++++       H+++   ++ +  ++  + ++   +   +P PK    E+I   ++M +L+  T  +   LMVRG    V+T
Sbjct:  871 GNIDIWWIVHDGGLLMLLPFLLKQHRTWKNCKLRIFSVAQHEDNSIQMKKDLKTFLYHLRIEADVEVVEMQDSDISEYTYERTLLMEQRNEMLKEMRVSDKRKGKMTRRLSLNKGVGEYKVVDDIII-------HTDKVTNEIEVETVIENSRADVPNIEDKIP-PKVKFSEEID-EVHMEFLEVLTEGLERVLMVRGGGREVIT 1066          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|187611452|sp|A2BFP5.1|S12A9_DANRE (RecName: Full=Solute carrier family 12 member 9)

HSP 1 Score: 278.485 bits (711), Expect = 1.305e-76
Identity = 254/968 (26.24%), Postives = 440/968 (45.45%), Query Frame = 0

Query: 1171 IYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGIS----------FIGMALVNKIQLLLLLVL-------------LASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLF-DVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLR---DNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLSFDMHLSVGVLRVQG---GLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFL--SNRPDH-LMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRG 2105
            +Y   G +  S        +     T P +     GV VP +L+++ ++LFLR  +VVG AGLL    ++ +A  + S+T +S+ A+ TNG ++GGG YFMISRSLGPEFGG++GLMF  A   A  +Y++G  EA++D+  Q               A  +R+L       +L  S           +G  +  K   L+LLV+             ++ Q   +  T+        +    GF+   L++NL P +  +     +  F  VFAV F + TG++AGANMSG+LK+P  +IPKGT+ A+  T+  Y++  L+++++  R        LLL+                     +  + Q  ++W P +  G + A+LS+A+ S++G  R+  A+A D+LF    +       S  P    L  +A+    +  G LN ++ +++ F++  YA ++ +     W ++P +RP+F++F+    LLG   C+V+M +++   + A+++ ++LL +F+  R+P  +WG  SQ+  F    K L  L+  +DHVK +RP+++++  NP     L  F N + K+  L  L HV   D++    D ++   + WL       +KAF  LT + S   G +  + ++GLG +  N L++GF +       + E+Y+       D     G  R +G   G+D+  +  AH   +  A        ++  S++  A+ +     K++ L+      F        +   +G    + ID      +T        VG++            L +  +L+    + + ++R+F    S   D   + + E+   LL K RI     I+  D           N   MV G     Q   VSE           F   +  + KE+S  +A+  + LP P  +      YL  LD ++R + P L + G
Sbjct:   13 VYSMMGNAEDSRGGSAGTGEASNPKTDPRKLNTFFGVMVPTILSMFSIVLFLRTGFVVGHAGLLHGLLMLFVAYFIISLTILSICAISTNGAVEGGGAYFMISRSLGPEFGGSIGLMFYLAKVCACGVYVLGLVEAIMDVFGQ---------DPGSSVAQGLRVLPQGYWYTVLYSSVVLLLCMLVCLVGAHIYAKASFLILLVVTVSLISIIISPLIVSPQGFNITHTYGNNHSVTVSPSYTGFNSTTLKNNLGPRYSLDYSTNTMMSFATVFAVMFTSCTGIMAGANMSGELKNPSESIPKGTIMAVAYTFTVYVLLYLLLSSTCDR-------SLLLN---------------------DYAVFQRVNVWPPFVTIGVYCASLSAAMCSMIGASRILHALALDQLFGLPLAPAAVTSSSGNPWVSVLYTWALVQCTLFAGQLNVIAGIVTVFYLLAYAAVDLACLALEWASAPNFRPTFQFFSWHTCLLGIISCVVMMFVINPVYSSASIVLLLLLLLFLHYRSPTSSWGYISQALIFHQVRKYLLMLDSRKDHVKFWRPQVLLMVSNPRSSCQLICFVNQL-KKGGLFVLGHVQIGDLDVLPADPVQPQYNFWLSLVDKLGVKAFVDLTLSPSVRQGTQHLLRITGLGGMKPNTLVLGFYDN-----SYPEDYFLQ-----DPVFCKGD-RSEGDNFGVDLPSLQ-AHFPPVRHAESPRALQPQEYVSIIQDAIKMG----KNICLARY---FFQLPPESKGATYMWGKDSMDTIDVWPTNLLTPGSASYADVGSL----------FLLQMACVLNMASGWRRARLRIFVCVESESEDQGWLAKEEQFRELLGKLRIRAAIKIVAWD-----------NVARMVRGPNTESQP--VSE----------DFLCAVNGLLKEHSSTAAVRFLYLPDPPSSCELSQQYLTQLDTLTRDLGPTLLIHG 890          

HSP 2 Score: 189.119 bits (479), Expect = 2.296e-47
Identity = 126/441 (28.57%), Postives = 236/441 (53.51%), Query Frame = 0

Query:  385 IAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTK-RLSLLESIQITEASLDFKKVDALKSAANEWL---NSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQD 821
            ++  + G+++  L++NL   Y+ D ++    +F +VFAV F + TGI+AGAN+SG+LK+P+ +IPKGT++A+  T+  Y++  L+++    RS             L ND                     Y + Q       ++ W P +  G + A+LS+A+ S++GA R+L A+A D+LF    + +    ++ +P    +  + +    +  G LN ++ +++ F++ AYA ++ +       S+P +RP+F++++    LLG I C+ +MF+++   +  + V ++LL +F+++R P  +WG  SQ+  F    K +  L    DHVK +RP+++++  NP     L+ F + L K  L +L  +QI +  LD    D ++   N WL   +   +K+F  +T + S+ +G +  L I GLG + PN +++GF  +
Sbjct:  241 VSPSYTGFNSTTLKNNLGPRYSLDYSTNTMMSFATVFAVMFTSCTGIMAGANMSGELKNPSESIPKGTIMAVAYTFTVYVLLYLLLSSTCDRSL------------LLND---------------------YAVFQR------VNVWPPFVTIGVYCASLSAAMCSMIGASRILHALALDQLFGLPLAPAAVTSSSGNPWVSVLYTWALVQCTLFAGQLNVIAGIVTVFYLLAYAAVDLACLALEWASAPNFRPTFQFFSWHTCLLGIISCVVMMFVINPVYSSASIVLLLLLLLFLHYRSPTSSWGYISQALIFHQVRKYLLMLDSRKDHVKFWRPQVLLMVSNPRSSCQLICFVNQLKKGGLFVLGHVQIGD--LDVLPADPVQPQYNFWLSLVDKLGVKAFVDLTLSPSVRQGTQHLLRITGLGGMKPNTLVLGFYDN 640          

HSP 3 Score: 117.472 bits (293), Expect = 5.547e-25
Identity = 58/137 (42.34%), Postives = 90/137 (65.69%), Query Frame = 0

Query:  180 NGNDAEKGVNGAEDSSEGKA--IKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQ 314
            N  D+  G  G  ++S  K    K   F GV +  +L+++ ++LFLR  +V+G AGLL GL ++ ++  +  +T +S+ A+STNG ++ GG Y+MISRSLGPEFGG+IG+MF LA   A  +Y++G  E++MD+  Q
Sbjct:   19 NAEDSRGGSAGTGEASNPKTDPRKLNTFFGVMVPTILSMFSIVLFLRTGFVVGHAGLLHGLLMLFVAYFIISLTILSICAISTNGAVEGGGAYFMISRSLGPEFGGSIGLMFYLAKVCACGVYVLGLVEAIMDVFGQ 155          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|74752435|sp|Q9BXP2.1|S12A9_HUMAN (RecName: Full=Solute carrier family 12 member 9; AltName: Full=Cation-chloride cotransporter 6; Short=hCCC6; AltName: Full=Cation-chloride cotransporter-interacting protein 1; Short=CCC-interacting protein 1; Short=hCIP1; AltName: Full=Potassium-chloride transporter 9; AltName: Full=WO3.3)

HSP 1 Score: 276.944 bits (707), Expect = 6.126e-76
Identity = 258/978 (26.38%), Postives = 440/978 (44.99%), Query Frame = 0

Query:  201 KFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIA----------------KGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWL---NSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFED-----GSESKMSVDSGLA-SPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRI--EFSDVI-LLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMT---LPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRG 1147
            K   F GV +  +L+++ +++FLR+ +V+G AGLL+ L ++ ++  +  +T +S+ A++TNG ++ GG Y+MISR+LGPE GG+IG+MF LAN    ++ ++G  ES++D+     T  +G+    +      L GS+ L L+  +  +G     R    L  LL+   +  ++ S +     +I                   F G++++ L+ NL   Y +D T+G   NF SVFAV F   TGI+AGAN+SG+LKDP+ AIP GT++A+  T+  Y++   + ++   R+    ++E                              +YG  ++      IS W PL+  G +A  LS++++SL+GA R+L A+A+D LF  I + ++      +P    +  + +  + +L G LN ++++++ F++ AYA ++ S       S+P +RP+F  ++    LLG   C+ +MFL+    A  + + + LL   +  R    +WG  SQ+  F    K +  L    DHVK +RP++++L GNP     L+  A+ L K+  L     +T   LD    D ++     WL   +   +K+F  +T + S+ +GA+  L I GLG + PN +++GF  D     A  ++++ T + AF                 S    S      +F   R     S ++LN +D         KM+ +  LA +   L   +L R     G  Q        L +L   G P                   G +D+  L+      + +  IL     +  ++LRIF      +        + ALLS+ RI  E  +V+        + + +   DF          SG             + DA    L         ++Q               +E  +  +T   LP P  +   YP Y+A L+  TR + P L+V G
Sbjct:   36 KLSTFLGVVVPTVLSMFSIVVFLRIGFVVGHAGLLQALAMLLVAYFILALTVLSVCAIATNGAVQGGGAYFMISRTLGPEVGGSIGLMFYLANVCGCAVSLLGLVESVLDVFGADATGPSGLRVLPQGYGWNLLYGSLLLGLVGGVCTLGAGLYARASF-LTFLLVSGSLASVLISFVAVGPRDIRLTPRPGPNGSSLPPRFGHFTGFNSSTLKDNLGAGYAEDYTTGAVMNFASVFAVLFNGCTGIMAGANMSGELKDPSRAIPLGTIVAVAYTFFVYVLLFFLSSFTCDRTL---LQE------------------------------DYGFFRA------ISLWPPLVLIGIYATALSASMSSLIGASRILHALARDDLFGVILAPAKVVSRGGNPWAAVLYSWGLVQLVLLAGKLNTLAAVVTVFYLVAYAAVDLSCLSLEWASAPNFRPTFSLFSWHTCLLGVASCLLMMFLISPGAAGGSLLLMGLLAALLTARGGPSSWGYVSQALLFHQVRKYLLRLDVRKDHVKFWRPQLLLLVGNPRGALPLLRLANQL-KKGGLYVLGHVTLGDLDSLPSDPVQPQYGAWLSLVDRAQVKAFVDLTLSPSVRQGAQHLLRISGLGGMKPNTLVLGFYDD-----APPQDHFLT-DPAFSEPAD------------STREGSSPALSTLFPPPRAPG--SPRALNPQDYVATVADALKMNKNVVLARASGALPPERLSRGSG--GTSQLHHVDVWPLNLLRPRGGP-------------------GYVDVCGLF-----LLQMATILGMVPAWHSARLRIFLCLGPREAPGAAEGRLRALLSQLRIRAEVQEVVWGEGAGAGEPEAEEEGDFV--------NSG-------------RGDAEAEALARSANALVRAQQGRGTGGGPGGPEGGDAEGPITALTFLYLPRPPADPARYPRYLALLETLTRDLGPTLLVHG 905          

HSP 2 Score: 274.248 bits (700), Expect = 4.422e-75
Identity = 257/952 (27.00%), Postives = 423/952 (44.43%), Query Frame = 0

Query: 1206 GVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSA----------TLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLI-------------------GTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKN-VDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLR---DNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGF------QERWWLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMT------AASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAE-KMASLL--LKFRIEFEDVI-ILSDVTKHPKKETMANFQTMVSGDEQGVQRPIVSEVNLLKHLDQ---TRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRG 2105
            GV VP +L+++ +++FLR+ +VVG AGLL + A++ +A  + ++T +S+ A+ TNG ++GGG YFMISR+LGPE GG++GLMF  A     ++ ++G  E++LD           + G      + +R+L              L L+ G+  +G  L  +   L  L++  S    LI                   G+ +PP    R     GF+   L+ NL   + ++   G  +    VFAV F   TG++AGANMSG+LKDP  AIP GT+ A+  T+  Y++   + + +  R          L +ED  +F+  S                   +W PL+  G +A  LS++++SL+G  R+  A+A+D LF  I +  +   R   P    L  + +    +L G LN ++++++ F++  YA ++ S     W ++P +RP+F  F+    LLG A C+++M L+  G A  +L+ + LL   +  R    +WG  SQ+  F    K L  L+  +DHVK +RP+L+++ GNP     L   AN + K+  L  L HV   D++    D ++     WL       +KAF  LT + S   G +  + +SGLG +  N L++GF      Q+ +   P F E                           S   G+   + T      A       + +D  + +  AL ++           KNV     S       +S G    + +    V+ +   R +    G +DV  L+      L +  IL     +   ++R+ FL   P      AE ++ +LL  L+ R E ++V+         P+ E   +F     GD +     +    N L    Q   T       E       ++AL  + LP P  +   Y  YLA L+ ++R + P L V G
Sbjct:   42 GVVVPTVLSMFSIVVFLRIGFVVGHAGLLQALAMLLVAYFILALTVLSVCAIATNGAVQGGGAYFMISRTLGPEVGGSIGLMFYLANVCGCAVSLLGLVESVLD-----------VFGADATGPSGLRVLPQGYGWNLLYGSLLLGLVGGVCTLGAGLYARASFLTFLLVSGSLASVLISFVAVGPRDIRLTPRPGPNGSSLPP----RFGHFTGFNSSTLKDNLGAGYAEDYTTGAVMNFASVFAVLFNGCTGIMAGANMSGELKDPSRAIPLGTIVAVAYTFFVYVLLFFLSSFTCDRT---------LLQEDYGFFRAIS-------------------LWPPLVLIGIYATALSASMSSLIGASRILHALARDDLFGVILAPAKVVSRGGNPWAAVLYSWGLVQLVLLAGKLNTLAAVVTVFYLVAYAAVDLSCLSLEWASAPNFRPTFSLFSWHTCLLGVASCLLMMFLISPGAAGGSLLLMGLLAALLTARGGPSSWGYVSQALLFHQVRKYLLRLDVRKDHVKFWRPQLLLLVGNPRGALPLLRLANQL-KKGGLYVLGHVTLGDLDSLPSDPVQPQYGAWLSLVDRAQVKAFVDLTLSPSVRQGAQHLLRISGLGGMKPNTLVLGFYDDAPPQDHFLTDPAFSEP------------------------ADSTREGSSPALSTLFPPPRAPGSPRALNPQDYVATVADALKMN-----------KNVVLARASGALPPERLSRGSGGTSQLHHVDVWPLNLLRPRGGP-GYVDVCGLF-----LLQMATILGMVPAWHSARLRI-FLCLGPREAPGAAEGRLRALLSQLRIRAEVQEVVWGEGAGAGEPEAEEEGDFVNSGRGDAEA--EALARSANALVRAQQGRGTGGGPGGPEGGDAEGPITALTFLYLPRPPADPARYPRYLALLETLTRDLGPTLLVHG 905          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|27151681|sp|Q63632.1|S12A4_RAT (RecName: Full=Solute carrier family 12 member 4; AltName: Full=Electroneutral potassium-chloride cotransporter 1; AltName: Full=Erythroid K-Cl cotransporter 1; Short=rKCC1; AltName: Full=Furosemide-sensitive K-Cl cotransporter)

HSP 1 Score: 271.552 bits (693), Expect = 3.010e-73
Identity = 208/706 (29.46%), Postives = 343/706 (48.58%), Query Frame = 0

Query:  182 NDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDF--NGIVD-SVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDE------------------EIAKGFVGYDTAVLESNLYN--AYTKDETSGVQHNFFSVFAVF-FPAVTGIVAGA---NL-SGDLKDPAV----AIPKGTLLAIVTTYVTYMIYGLMVAWCSI-----RSASGNVEEVHFGTDLFNDT----VGEILNITQA----------YDDC-EG--RKCNYGILQSQQMMEVISAWGP--LIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVL----SGNPVDRPSLMDFAHLLT--KRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQS 825
             +AE G  G   ++  KA   G   GVY+ CL NI+GV+LFLRLTW++G AG+L+ L I+ +    T++T ISMSA++TNG + AGG Y+MISRSLGPEFGGA+G+ F L  + A +MYI+G  E L+  +      F  +G  D S   +N++R+ G+I L  +  +  VG+++V +     L  +++S +   +G +  + D                   +I    V  D   + + L+    ++ + T+     +F +  V   P + G  AG    NL S  L+   V     +P    L +  +   Y++  +  ++  +      S +G +   +   DL +      VG IL I             +  C EG   +  YG   S+ ++    AW    +I  G F +T  + + SL GAPR+LQA+AKD + P +  F  G  AN +P    +L  +I+ + ILI  L+ V+ +LS FF+  Y  +N +    ++  +P WRP FKYY+  +S LG  LC+ +MF+  +  A++  V   ++Y +I ++  +  WG   +  S   A  A+  L     H KN+RP+++VL        V  P L+ FA  L   K L+++ S+        + +  A +      +    +K F  +     + EG    ++  GLG +  N V++G+   W+QS
Sbjct:  100 EEAESGEGGRRRAA--KAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLFLACVIISILSIYVGGIKSAFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNLTADSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHG-KANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFVSSWYYALVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQS 802          

HSP 2 Score: 167.933 bits (424), Expect = 2.122e-40
Identity = 118/396 (29.80%), Postives = 190/396 (47.98%), Query Frame = 0

Query: 1421 AVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSD----RPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKA--FVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERW 1810
             +FFP+VTG++AG+N SGDL+D   +IP GT+ AI+TT       +LV  +S +   +     +L D+     + +  +RN   GTL         S W  +I  G F +T  + L SL G PR+ QAIAKD + P++  FG G     EP    LL   IA   ILI  L+ V+ +LS FF+  Y  +N +    +   +P WRP FKY++  +S LG +LC+ +M +     AL  ++   ++Y ++  +  +  WG   +  S   A  +L  L     H KN+RP+L+V+     D     P L  FA+ +     L  +  VI+        +   A   + + +    +K F  +        G+   ++  GLG +  N +++G+   W
Sbjct:  425 GIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTT-------SLVYFSSVILFGACIEGVVLRDK-----YGDGVSRNLVVGTL------AWPSPW--VIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANG-EPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFVSSWYYALVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGW 799          

HSP 3 Score: 151.754 bits (382), Expect = 2.037e-35
Identity = 85/185 (45.95%), Postives = 113/185 (61.08%), Query Frame = 0

Query: 1202 GWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF--DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIG----TFVPP 1380
            G + GVY+PCL NI+GV+LFLRLTW+VG AG+L +  I+ I    T +TAISMSA+ TNG +  GG YFMISRSLGPEFGGAVGL F    + A +MYI+G  E LL  I   ++ F   G    S    N++R+ G+  L  +  + F+G+  VNK   L L  ++ S +   +G     F PP
Sbjct:  119 GTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLFLACVIISILSIYVGGIKSAFDPP 303          

HSP 4 Score: 60.4622 bits (145), Expect = 1.648e-7
Identity = 27/84 (32.14%), Postives = 51/84 (60.71%), Query Frame = 0

Query: 1938 GTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIIL---SDVTKHPKKETM 2018
            G IDV+W+  DGG+ +LLP++L   K + KC+MR+F ++   D+ ++  + +A  L   R+E E  ++    SD++ +  + T+
Sbjct:  844 GHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTL 927          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|27151689|sp|Q9JIS8.2|S12A4_MOUSE (RecName: Full=Solute carrier family 12 member 4; AltName: Full=Electroneutral potassium-chloride cotransporter 1; AltName: Full=Erythroid K-Cl cotransporter 1; Short=mKCC1)

HSP 1 Score: 270.011 bits (689), Expect = 9.096e-73
Identity = 211/706 (29.89%), Postives = 346/706 (49.01%), Query Frame = 0

Query:  182 NDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDF--NGIVD-SVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGS--------VIP---------SSDE-EIAKGFVGYDTAVLESNLYN--AYTKDETSGVQHNFFSVFAVF-FPAVTGIVAGA---NL-SGDLKDPAV----AIPKGTLLAIVTTYVTYMIYGLMVAWCSI-----RSASGNVEEVHFGTDLFNDT----VGEILNITQA----------YDDC-EG--RKCNYGILQSQQMMEVISAWGP--LIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVL----SGNPVDRPSLMDFAHLLT--KRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQS 825
             +AE G  G   ++  KA   G   GVY+ CL NI+GV+LFLRLTW++G AG+L+ L I+ +    T++T ISMSA++TNG + AGG Y+MISRSLGPEFGGA+G+ F L  + A +MYI+G  E L+  +      F  +G  D S   +N++R+ G+I L L+  +  VG+++V +     L  +++S +    G         V P         S D+ +I    V  D   + + L+    ++ + T+     +F +  V   P + G  AG    NL S  L+   V     +P    L +  +   Y++  +  ++  +      S +G +   +   DL +      VG IL I             +  C EG   +  YG   S+ ++    AW    +I  G F +T  + + SL GAPR+LQA+AKD + P +  F  G  AN +P    +L  +I+ + ILI  L+ V+ +LS FF+  Y  +N +    ++  +P WRP FKYY+  +S LG  LC+ +MF+  +  A++  +   ++Y +I ++  +  WG   +  S   A  A+  L     H KN+RP+++VL        V  P L+ FA  L   K L+++ S+        + +  A +      ++   +K F  +     + EG    ++  GLG +  N V++G+   W+QS
Sbjct:  100 EEAESGEGGRRRAA--KAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNLTADSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHG-KANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQS 802          

HSP 2 Score: 166.392 bits (420), Expect = 5.307e-40
Identity = 118/396 (29.80%), Postives = 190/396 (47.98%), Query Frame = 0

Query: 1421 AVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSD----RPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKA--FVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERW 1810
             +FFP+VTG++AG+N SGDL+D   +IP GT+ AI+TT       +LV  +S +   +     +L D+     + +  +RN   GTL         S W  +I  G F +T  + L SL G PR+ QAIAKD + P++  FG G     EP    LL   IA   ILI  L+ V+ +LS FF+  Y  +N +    +   +P WRP FKY++  +S LG +LC+ +M +     AL  ++   ++Y ++  +  +  WG   +  S   A  +L  L     H KN+RP+L+V+     D     P L  FA+ +     L  +  VI+        +   A   + + +    +K F  +        G+   ++  GLG +  N +++G+   W
Sbjct:  425 GIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTT-------SLVYFSSVILFGACIEGVVLRDK-----YGDGVSRNLVVGTL------AWPSPW--VIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANG-EPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGW 799          

HSP 3 Score: 150.984 bits (380), Expect = 2.990e-35
Identity = 86/185 (46.49%), Postives = 113/185 (61.08%), Query Frame = 0

Query: 1202 GWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF--DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIG----TFVPP 1380
            G + GVY+PCL NI+GV+LFLRLTW+VG AG+L +  I+ I    T +TAISMSA+ TNG +  GG YFMISRSLGPEFGGAVGL F    + A +MYI+G  E LL  I   ++ F   G    S    N++R+ G+  L L+  + F+G+  VNK   L L  ++ S +    G     F PP
Sbjct:  119 GTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPP 303          

HSP 4 Score: 60.4622 bits (145), Expect = 1.734e-7
Identity = 27/84 (32.14%), Postives = 51/84 (60.71%), Query Frame = 0

Query: 1938 GTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIIL---SDVTKHPKKETM 2018
            G IDV+W+  DGG+ +LLP++L   K + KC+MR+F ++   D+ ++  + +A  L   R+E E  ++    SD++ +  + T+
Sbjct:  844 GHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTL 927          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|544584826|sp|P34261.6|YKAA_CAEEL (RecName: Full=Uncharacterized amino-acid permease B0303.11)

HSP 1 Score: 263.848 bits (673), Expect = 1.296e-71
Identity = 250/996 (25.10%), Postives = 455/996 (45.68%), Query Frame = 0

Query:  190 GAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHF--GTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILC-IGVMF----LMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLES---IQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDG-GLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRD---GSVLGRFQKKTKSSMS------------LAILDKD--GRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAK-SKLRIFFLSENVD--ELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVD-KFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSV 1152
             +ED+         W D V+++C+  +  V+L LR + ++ +AG    + ++  + +V+++T  S   V +    + G +  M++ S   EF  +  I++     +A S ++    E+++ +   +  +   ++D     +D+RL+ S+  ++ LAL +V       V+  +  L  ++ +   + SV+    E   +  V    A++ S        +E S       ++FA  FPA    +   N+   L++ A   P+G L+AI    V+   YG          A+  ++ V F   T   N T                     G  +  + +  I    P+      A  LS A+++L  A  +LQ++ +      I   ++G+G  + P+R  +L+  + ++   IG  + +    + F++ AYAL NF VF   ++      P        +SL  +  C I  ++    L  F  +I       LLY+    R+ +   G       + + L+ +  L +  D  +++ P+I++LSG+P  RP L+DFAH +T+  SLL     I  +  S  +     +    N+WL +  + +F +        EGA   L+  GLG+L PN++++G+K  W++ S     EYY  L  AFD ++ V + R +  G D+                   TS + +      D  +    VDS  A+P    +    +D   G +L  F+K + + +               ++DK     P  +  + ++ +FR K I +  ID++WL + GGLTML P++LT    F + + +R+F  ++  D   ++E+ +NM A+L KF I+ SD+ +L + +K   K+T D+F+A I           ++S   E VD  FD          ++    E+T   LR +E++R++SS+ADL+V TLP   R +IP P+Y+ W+D  +R+ PP  +VRGNQ S+
Sbjct:    7 SSEDAPITSTAWMNWKD-VFLKCVQPMLAVVLLLRFSSIVDEAGFTTTIILVFFTFLVSLVTGWSACTVVSRKSSEVGFVKTMLAYS-STEFAISFSIIYLFCLLVATSTFLTSAAEAVLHIFSTFSLE---LLDGAT--HDLRLVSSVLSLITLALCMVRNRNARFVRTFIFALTCIA-IALQLSSVMFRYGEYQLRR-VSDRNAMIPSP-----PNEEIS-------TIFAQLFPAAMCGLTILNIGSKLQNTA---PRGALIAIA---VSACFYG----------AAAMLDYVEFFARTSTSNST---------------------GSAEYNEFLSYIYTTVPMAIVITLACVLS-AVSTLKYAAVILQSLGRSNQCRCILWLAKGFGERDIPIRCLLLLSTVQILVSAIGSYDILCIPTTVFYLFAYALFNFYVFLVKLSD-----PEIPSPPTLLSLAISAACFIASLYTNRHLALFIASIFAISYCSLLYII---RRERNEDGEECPKSMYSSVLEQMHELQQEPDSRRHFHPQILLLSGSPAARPGLVDFAHSITRGKSLLICGYIIPQSPCSRSYLLQLKIDKQINDWLRAREVNAFGAAICCTKQAEGANILLQTAGLGRLRPNILMLGYKTGWEKLSKESISEYYGMLSNAFDKQVGVIIFRNEASGFDV-------------------TSSIRKNGAPINDDEDLAEYVDS--ATPKLADQGSQKKDVPRGKLLNTFRKMSMADLESGGRRSTSSSTRFQVIDKHSISEPDQKIIMAQMFRFR-KRIPNARIDVFWLREAGGLTMLAPYLLTQAGSFLEGAHIRVFTKTDGKDNKRINEEQKNMAAILRKFHIDSSDLHILPEFSKPPCKQTYDEFRAKI------DKYKVETSSSGEPVDGSFD--------NNQIFNLREKTRSFLRASELIREHSSDADLIVCTLP-SARPEIPSPIYLGWIDMLSRQTPPTCLVRGNQVSM 898          

HSP 2 Score: 231.106 bits (588), Expect = 7.260e-61
Identity = 244/960 (25.42%), Postives = 425/960 (44.27%), Query Frame = 0

Query: 1196 TGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF-DGIVGDSEHRANDVRILGSATLVLLLGISFI---GMALVNKIQLLLLLVLLA---SQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTAL--ATLICII---LLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPR---IMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQG------------GLDISDISGAHEKVMTA----ASQASGASDEDRFSMLN--HALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAK-CKMRVFFLSNRPDH--LMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGDEQGVQ-----RPIVSEVN---LLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESV 2110
            T  A   W + V++ C+  +  V+L LR + +V +AG   +  ++    LV+ VT  S   V +    + G +  M++ S   EF  +  +++     +A S ++    EA+L +   FS +  DG         +D+R++ S   ++ L +  +       V      L  + +A   S V F  G +      +R            R+ + P    +   E++    +FA  FPA    +   N+   L++     P+G L AI  + C Y   A+                  LD  +  +F   S  N T    +N+ +  + +   P+      A  LS A+++L     + Q++ +      I    +GFG    P+R  LL+  + +    IG  + +    + F++  YA+ N+ VF    ++     P        +SL  +A C +  +  +   AL  A++  I    LLYI  R RN D   G       + + L+ +  L    D  +++ P++++++G+P+ RP L DFA+ IT+  SLL   ++I +    R   +   +   ++ WLR   + AF A         G    ++ +GLG+L  N+LM+G++  W    K    EYY +L  +FD  + V + R +             G  I+D     E V +A    A Q S   D  R  +LN    +S+ + +    + S+ +   F      + SE         P  + I+ +M +FR +      IDV+WL + GGLT+L PY+L+    F +   +RVF  ++  D+  + EE + MA++L KF I+  D+ IL + +K P K+T   F+  +  D+  V+      P+    +   +    ++TR  LR +E+ +E+S  + L+V TLP  R  EIP  +YL W+D +SR  PP   VRGNQ S+
Sbjct:   14 TSTAWMNW-KDVFLKCVQPMLAVVLLLRFSSIVDEAGFTTTIILVFFTFLVSLVTGWSACTVVSRKSSEVGFVKTMLAYS-STEFAISFSIIYLFCLLVATSTFLTSAAEAVLHIFSTFSLELLDGAT-------HDLRLVSSVLSLITLALCMVRNRNARFVRTFIFALTCIAIALQLSSVMFRYGEY----QLRRVSD---------RNAMIP----SPPNEEIS--TIFAQLFPAAMCGLTILNIGSKLQNTA---PRGALIAIAVSACFYGAAAM------------------LDYVE--FFARTSTSNSTGSAEYNEFLSYIYTTV-PMAIVITLACVLS-AVSTLKYAAVILQSLGRSNQCRCILWLAKGFGERDIPIRCLLLLSTVQILVSAIGSYDILCIPTTVFYLFAYALFNFYVFLVKLSD-----PEIPSPPTLLSLAISAACFIASLYTNRHLALFIASIFAISYCSLLYIIRRERNED---GEECPKSMYSSVLEQMHELQQEPDSRRHFHPQILLLSGSPAARPGLVDFAHSITRGKSLLICGYIIPQSPCSRSYLLQLKIDKQINDWLRAREVNAFGAAICCTKQAEGANILLQTAGLGRLRPNILMLGYKTGWEKLSKESISEYYGMLSNAFDKQVGVIIFRNEASGFDVTSSIRKNGAPINDDEDLAEYVDSATPKLADQGSQKKDVPRGKLLNTFRKMSMADLE-SGGRRSTSSSTRFQVIDKHSISE---------PDQKIIMAQMFRFRKRIPN-ARIDVFWLREAGGLTMLAPYLLTQAGSFLEGAHIRVFTKTDGKDNKRINEEQKNMAAILRKFHIDSSDLHILPEFSKPPCKQTYDEFRAKI--DKYKVETSSSGEPVDGSFDNNQIFNLREKTRSFLRASELIREHSSDADLIVCTLPSARP-EIPSPIYLGWIDMLSRQTPPTCLVRGNQVSM 898          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1339058872|ref|XP_023717973.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Cryptotermes secundus])

HSP 1 Score: 1029.62 bits (2661), Expect = 0.000e+0
Identity = 538/1050 (51.24%), Postives = 715/1050 (68.10%), Query Frame = 0

Query:  138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEE---YYRTLETAFDMRLAVGVLRIDGGLDISEMFASDE----------KAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKD-------------------GRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            LT+EV P V+HYR+    S  H  + RPTLDEL + ++    +G D  +    A+   +GK IKFGW +GV+MRCLLNIWGVMLFLRL+WV+GQAG+ EG  II L+NIVT++TTISMSAV TNGQIK GGIYYMISRSLGPEFGGAIG+MFTLANS+AVSMYI+GFCESL D+LR + T    ++D     ND+R++G ++L+ IL LAIVGM+WVTR QM LL +L+ SQ+DF++GS + P  DEEIAKGFVGY++    SNL   Y   E  GV H+FFSVFAVFFPAVTGIVAGANLSGDLKDP+ AIP GTLLAI  T+++Y+ Y  M+  C +R+ASGNV E       +    GE  +    + +C  R C YG+    Q ME+ S+WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I  FS+G GANNDPVRGY+LVF+IS  CILI  LN ++ LLSNFF+AAYALINFSVFHASI+ SPGWRP+FKYYN W+SL GT+LCI VMFLM +  A+IT V +I LY+++ +RKP+VNWGSS+Q+ S+ +ALKA+  L RV++HVKN+RP+++VL+G P  RP L+DFA+L+TK LSLL    I + +   +  DAL   A  WL  + +K+F +I  +DS   G++A ++I GLGKL PNM+LMG+K +W+    D EE   Y+  +  A D  LAVG+LR+  GLD S +   +E           A+    RN +T++L  Q +N    S++ +S D+  + P T   +  G         +K  +SS ++ I  K                    G P+ +  +  ITQF+ K  + G ID++WLYDDGGL +L+P+IL+TR++F+  +LRIF L+   D+LD+D RN+ ALLSKFRI++SDV+++ D+ KKA + TR +F+  I     K           E  +   A+E   +T+ EL    E+TN  +RI E++ Q S E+  +VM LP+P++  +  PLYMAWL+  T+ MPP+L++RGNQ SV+T
Sbjct:   70 LTREVPPRVEHYRD---LSIQH--QYRPTLDELHERNIPEKSDGEDKVE----AQQQKKGKVIKFGWLEGVFMRCLLNIWGVMLFLRLSWVVGQAGIGEGTAIICLANIVTLVTTISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRTFGTT---LIDGAS--NDIRVVGLVTLIGILVLAIVGMDWVTRTQMCLLFVLIASQIDFMVGSFLGPQDDEEIAKGFVGYNSETFRSNLGPDYRNYE--GVDHDFFSVFAVFFPAVTGIVAGANLSGDLKDPSSAIPVGTLLAIFVTFLSYIGYAFMMGGCVLRTASGNVTE-------YLQVKGEP-DEWAVFSNCTDRSCLYGLHNDSQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSKGHGANNDPVRGYVLVFVISFGCILIAQLNAIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLAGTLLCIAVMFLMSWVMALITFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCADILKGTHSQRVHDALTRKAYHWLIRHKVKAFFNIVEDDSFEAGSQALMQISGLGKLRPNMILMGYKGNWRT--CDREELKQYFNVIHNALDRYLAVGILRVQEGLDYSGVIQDEEFVPISKLDKKDAEEKMRRNESTNQLESQDVNITVLSDA-LSSDTD-SPPVTPKMQHGGTVNCDPAATEKLLQSSTTVTITSKKMRKVSKNHKSSFPTYVGPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLALLIPYILSTRSQFSGCQLRIFSLASKRDDLDKDQRNLAALLSKFRIDYSDVVIISDIHKKAMESTRKEFEIFIQPFRVKD---------EENFETSKAIEQTYITDSELIALREKTNRHMRIRELLLQYSKESTFIVMALPIPRKGVVSAPLYMAWLELLTKGMPPYLIIRGNQTSVIT 1081          

HSP 2 Score: 907.516 bits (2344), Expect = 0.000e+0
Identity = 488/1091 (44.73%), Postives = 685/1091 (62.79%), Query Frame = 0

Query: 1081 EEELQRHSEQ--TNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF-DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLL---DREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDIS--------------DISGAHEKVMTAASQASGASDEDRFSMLNHALSLDE----FQPKSVQLSSKNVDSFGT--------------------SSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS----GDEQ------GVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            ++ELQ   E   T FQ+ I +    +  E D +  + P  +   +    Y +W   T R++PP +    +       RPTLDEL    RN    P +        A  Q K  V K      FGW++GV++ CLLNIWGVMLFLRL+WVVGQAG+    AII +AN+VT VT ISMSAVCTNGQIKGGGIY+MISRSLGPEFGGA+GLMFT A S+AVSMYI+GFCE+L D++  F +   DG        +ND+R++G  TL+ +L ++ +GM  V + Q+ LL VL+ASQ+DF++G+F+ P  DE+ A G VG++ +  RSNL PD+ +N +G     F VFAVFFPAVTG+VAGAN+SGDLKDP SAIP GTL AI  T+ SY+ YA ++    +R ASGN  E L    + ++   F NC++R+C YG   + Q ++L+S WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P+I  F +G G + +P+RGY+L+F I+  CILI  LN ++ LLSNFF+A YA+IN+SVFHAS + SPGWRP+FKY+N W+SL GT LCI VM LM    AL T + +I LY++V  R P+VNWGSS+Q+ S+ +ALK++  LN VE+HVKN+RP+++V+TG P  RP L DFA LITK  SLL    ++K     R+ D +    +HWL  + +KAF+ +  +DSF  G +  +++SGLGKL  NM+++G++  W    +   ++Y+ V+  + D +L+VG+LRVQ GLD S              D   A EK+    S     S +   ++L+ ALS D       PK     + N D   T                    S    +S  +Y    G P+ + I+  +TQF+ K  K G IDV+WLYDDGGL LL+PYILSTR +F+ C++R+F L+++ D L ++   +A+LL KFRI++ DV+I+SD+ K   + T   F+  +      DE+       +++  +++  L+   ++T  H+RI E+  + S  S  +VM LP+PR+  +   LY+AWL+ +++ MPP+L +RGNQ SV+T YS
Sbjct:   16 QQELQDIDENGSTRFQVDIVD-AENDDKENDYMTSSTPNQQYSYVYDSRYKSWGQLT-REVPPRVEHYRDLSIQHQYRPTLDEL--HERN---IPEKSDGEDKVEAQQQKKGKVIK------FGWLEGVFMRCLLNIWGVMLFLRLSWVVGQAGIGEGTAIICLANIVTLVTTISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRTFGTTLIDGA-------SNDIRVVGLVTLIGILVLAIVGMDWVTRTQMCLLFVLIASQIDFMVGSFLGPQDDEEIAKGFVGYNSETFRSNLGPDY-RNYEGVDHDFFSVFAVFFPAVTGIVAGANLSGDLKDPSSAIPVGTLLAIFVTFLSYIGYAFMMGGCVLRTASGNVTEYLQVKGEPDEWAVFSNCTDRSCLYGLHNDSQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSKGHGANNDPVRGYVLVFVISFGCILIAQLNAIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLAGTLLCIAVMFLMSWVMALITFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCADILKGTHSQRVHDALTRKAYHWLIRHKVKAFFNIVEDDSFEAGSQALMQISGLGKLRPNMILMGYKGNWRTCDREELKQYFNVIHNALDRYLAVGILRVQEGLDYSGVIQDEEFVPISKLDKKDAEEKMRRNESTNQLESQDVNITVLSDALSSDTDSPPVTPKMQHGGTVNCDPAATEKLLQSSTTVTITSKKMRKVSKNHKSSFPTYVGPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLALLIPYILSTRSQFSGCQLRIFSLASKRDDLDKDQRNLAALLSKFRIDYSDVVIISDIHKKAMESTRKEFEIFIQPFRVKDEENFETSKAIEQTYITDSELIALREKTNRHMRIRELLLQYSKESTFIVMALPIPRKGVVSAPLYMAWLELLTKGMPPYLIIRGNQTSVITVYS 1084          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1228359575|ref|XP_021952830.1| (solute carrier family 12 member 3-like [Folsomia candida])

HSP 1 Score: 1017.3 bits (2629), Expect = 0.000e+0
Identity = 529/1039 (50.91%), Postives = 713/1039 (68.62%), Query Frame = 0

Query:  138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYN----AYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQ-----AYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDW-QQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATL-----TKRKLGRDGSVLGRF------QKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            LT+E LP +D+YR+ LS         RPTLDEL + +        D E G     +  +GK IKFGW +GV MRC+LNIWGVMLFLRL+W +GQAG+LEG  +I+L+N+VT IT  SMSAVSTNGQIK GGIYYMISRSLGPEFG AIG+MFT+ANSIAVS+YI+GFCESL D+L+    ++  ++D     ND+R+IG   LV IL LA VGM+WVTR QM LL++L+ +QV+FIIGS I P  D E AKGF GY+     +N ++    AY +D T   + +F++VF VFFPAVTGIVAGANLSGDLKDPA AIPKGTLLAI  TY++Y+ Y +M+  C++R ASGN+ E       +N TV   + + Q     AYD+C  R C YG+  S Q+ME+IS++G LIYAGCFAATLSSAIASLVGAPRV QA+ KDKL+P IH F++G+GANNDPVRGY LVF+ISL C+LI +LN ++ LLSNFF+ AY LINFSVFHASI+ SPGWRP+FKYYN W+SLLG +LCI VMFL+++ TA+ T  C++ LY+++ +RKP+VNWGSS+Q+QSF +ALK++Q L  V++HVKNYRP+I+V++G P +RP L+DFA+L+ K LSL+    + +     K   AL   A  WL  +  K F+++  ++S   GA++ ++I GLGKL PNMVLMG+K DW + +  D +EY+  +  A DM LAVG+LR   GLD S +   +E+ Q+     +   E+ ++     + SES++S D   +  ++       +RK   D  V  +       +K  K S+S       G  +    +  ITQF+ K  + GTID++WLYDDGGL++LLP++LTTR ++A   LRIF L+   DEL  + RNM ALLSKFRI++SDVI++ D+TKKA+ KT+ +F+++I            +  KS+     D  +   +TE EL  H E+TN  LR+ E+++ +S ++  VVMTLP+P++  +  PLYMAWL+  TR MPPFL++RGNQ SVLT
Sbjct:   74 LTREALPALDNYRDLLSVHAA----PRPTLDELHNATYTEKTKAMDEETG-----EVLKGKVIKFGWLEGVLMRCMLNIWGVMLFLRLSWCVGQAGILEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTIANSIAVSLYIVGFCESLNDLLKA--NNWGQLIDGGS--NDIRVIGVGVLVAILVLAFVGMDWVTRTQMFLLVVLIAAQVNFIIGSFIGPVDDLEKAKGFPGYNAETFVTNFWSDYRTAYGEDTT---KQDFWTVFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYLSYIGYAVMMGGCAMREASGNLTE-------YNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVI--EEEGQLT---PQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPDQVVNNQLVGFSSNKKSRKHSLSAMYKGPGGAILPRHVLSNITQFQRKQ-KKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVI------------AEFKSD-----DNQDGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFVVMTLPMPRKGTVSAPLYMAWLEMLTRDMPPFLLLRGNQTSVLT 1066          

HSP 2 Score: 895.19 bits (2312), Expect = 0.000e+0
Identity = 462/996 (46.39%), Postives = 655/996 (65.76%), Query Frame = 0

Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIY--QFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNV--DGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGN---------PEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLD-------------EFQPKSVQLSSKNVDSFGTSSTRNNSEVS----YGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV----SGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            RPTLDEL     + + Y  + ++    T +      +K       FGW++GV + C+LNIWGVMLFLRL+W VGQAG+L  F +I++ANLVT +TA SMSAV TNGQIKGGGIY+MISRSLGPEFG A+GLMFT A SIAVS+YI+GFCE+L D++    +    DG        +ND+R++G   LV +L ++F+GM  V + Q+ LL+VL+A+QV+F+IG+F+ P D+ ++A G  G++ +   +N W D++     D  +   + VF VFFPAVTG+VAGAN+SGDLKDP SAIPKGTL AI  TY SY+ YA+++    MR ASGN          E  +L  E  + + NC+NR+C YG   + Q+++L S +G LIYAGCFAATLSSA+ASLVG PRVFQA+ KDKL+P I  F +G+G + +P+RGY L+F I+L C+LI +LN ++ LLSNFF+  Y +IN+SVFHAS ++SPGWRP+FKY+N W+SLLG  LCI VM L++  TALAT+ C++ LY++V  R P+VNWGSS+Q+QSF +ALKS+Q LN+VE+HVKNYRP+++V+TG P +RP L DFA LI K  SL+   HV+K     ++   +    + WL  +  K FY L  ++SF  G ++ +++ GLGKL  NM+++G++  W    +   +EY+ V+  + DMHL+VG+LR   GLD S++     ++     Q      E   +     LS D             + + K++     N    G SS + + + S    Y    G  +   ++  +TQF+ K QK GTIDV+WLYDDGGL++LLPY+L+TRK++A C +R+F L++R D L  E   MA+LL KFRI++ DVI++ D+TK  + +T   F++++    S D Q      ++E  L+ H ++T  HLR+ E+ + +S  S  VVMTLP+PR+  +   LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct:   96 RPTLDEL-----HNATYTEKTKAMDEETGEVLKGKVIK-------FGWLEGVLMRCMLNIWGVMLFLRLSWCVGQAGILEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTIANSIAVSLYIVGFCESLNDLLKANNWGQLIDG-------GSNDIRVIGVGVLVAILVLAFVGMDWVTRTQMFLLVVLIAAQVNFIIGSFIGPVDDLEKAKGFPGYNAETFVTNFWSDYRTAYGEDTTKQDFWTVFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYLSYIGYAVMMGGCAMREASGNLTEYNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVIEEEGQLTPQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPDQVVNNQLVGFSSNKKSRKHSLSAMYKGPGGAILPRHVLSNITQFQRK-QKKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVIAEFKSDDNQDGTS--ITESELMSHREKTNRHLRLRELLQLHSKDSTFVVMTLPMPRKGTVSAPLYMAWLEMLTRDMPPFLLLRGNQTSVLTFYS 1069          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1228009301|ref|XP_021936563.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X2 [Zootermopsis nevadensis])

HSP 1 Score: 1011.13 bits (2613), Expect = 0.000e+0
Identity = 543/1039 (52.26%), Postives = 710/1039 (68.33%), Query Frame = 0

Query:  138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEE---YYRTLETAFDMRLAVGVLRIDGGLDISEMFASDE----------KAQMIFARNRTTSELSEQSLNF--------EDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            LT+EV P V+HYR+ LS    H E  RPTL+EL  E ++ +    + E      ++  +GK IKFGW +GV+MRCLLNIWGVMLFLRL+WVIGQAG+ EG  II L+NIVT++T+ISMSAV TNGQIK GGIYYMISRSLGPEFGGAIG+MFTLANS+AVSMYI+GFCESL D+LR + T    I+D     ND+R++G ++L+ I  LAIVGM+WVTR QM LL +L+ SQ+DF+IGS + P  DEEI KGFVGY+     SNLY  Y   E  GV H+FFSVF+VFFPAVTGIVAGANLSGDLKDP+ AIP GTLLAIV T+++Y+ YG M+  C +R+ASGNV E       +    GE  N    + +C  R C YG+    Q ME+ S+WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I  FS+G GANNDPVRGYILVF+IS  CILI  LN ++ LLSNFF+AAYALINFSVFHASI+ SPGWRP+FKYYN W+SL+GT+LCI VMFLM +  A+ T V +I LY+++ +RKP+VNWGSS+Q+ S+ +ALKA+  L RV++HVKN+RP+++VL+G P  RP L+DFA+L+TK LSLL    I +A+   +  +AL   A +WL  + +K+F  IT +DS   G +  ++I GLGKL PNM+LMG+K +W+    D EE   Y+  +  A D  LAVG+LR+  GLD S +   +E           A+    RN+++++L  Q +N          D     M+ D  +AS + L         S   R   KT+ S     +   G P+ +  +  ITQF+ K  + G ID++WLYDDGGLT+L+P+IL+TR++F+  +LRIF L+   D+LD D RN+ ALLSKFRI++SDV+++ D+ KKA   TR +F+  I     K G  S +S  S             +TE EL    E+TN  +RI E++ Q S E+  +VM LP+P+++ +  PLYMAWL+  T+ MPP L++RGNQ SV+T
Sbjct:   65 LTREVPPRVEHYRH-LSI---HHEY-RPTLEEL-HERIIPEKRERNVEAE---PQEQKKGKVIKFGWLEGVFMRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRSFGTT---IIDGAS--NDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNLYPDYRNYE--GVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTE-------YLQVKGEP-NEWTIFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRT--CDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDP-VASDSLLESSTTVTVTSKKSRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTS---------NKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGMPPCLIIRGNQTSVIT 1066          

HSP 2 Score: 880.937 bits (2275), Expect = 0.000e+0
Identity = 474/1026 (46.20%), Postives = 661/1026 (64.42%), Query Frame = 0

Query: 1136 TRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF-DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLL---DREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLY-PKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDIS--------------DISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPK----------------SVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS------GDEQGV----QRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            TR++PP +    +       RPTL+EL  R     I P +   N       Q K  V K      FGW++GV++ CLLNIWGVMLFLRL+WV+GQAG+    AII +AN+VT VT+ISMSAVCTNGQIKGGGIY+MISRSLGPEFGGA+GLMFT A S+AVSMYI+GFCE+L D++  F +   DG        +ND+R++G  TL+ +  ++ +GM  V + Q+ LL VL+ASQ+DF+IG+F+ P  DE+   G VG++ +   SNL+PD+ +N +G     F VF+VFFPAVTG+VAGAN+SGDLKDP SAIP GTL AI+ T+ SY+ Y  ++    +R ASGN  E L    +  +   F NC++R C YG   + Q ++L+S WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P I  F +G G + +P+RGY+L+F I+  CILI  LN ++ LLSNFF+A YA+IN+SVFHAS + SPGWRP+FKY+N W+SL+GT LCI VM LM    AL T + +I LY++V  R P+VNWGSS+Q+ S+ +ALK++  LN VE+HVKN+RP+++V+TG P  RP L DFA LITK  SLL   H++K     R+ + +    + WL  + +KAF+ +T +DSF  G +  +++SGLGKL  NM+++G++  W    P+  ++Y+ V+  + D +L+VG+LRVQ GLD S              D   A EK+    S     S +   S+L+ AL+ D   P                 +V ++SK   S  T  T+ ++   Y    G P+ + I+  +TQF+ K  K G IDV+WLYDDGGLTLL+PYILSTR +F+ C++R+F L+++ D L  +   +A+LL KFRI++ DV+I+SD+ K     T   F+T +       GD         +  ++E  LL   ++T  H+RI E+  + S  S  +VM LP+PR++ +   LY+AWL+ +++ MPP L +RGNQ SV+T YS
Sbjct:   66 TREVPPRVEHYRHLSIHHEYRPTLEELHER-----IIPEKRERNVEAEPQEQKKGKVIK------FGWLEGVFMRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRSFGTTIIDG-------ASNDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNLYPDY-RNYEGVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTEYLQVKGEPNEWTIFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRTCDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDPVASDSLLESSTTVTVTSKK--SRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGMPPCLIIRGNQTSVITVYS 1069          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1228009299|ref|XP_021936562.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X1 [Zootermopsis nevadensis])

HSP 1 Score: 1010.75 bits (2612), Expect = 0.000e+0
Identity = 543/1039 (52.26%), Postives = 710/1039 (68.33%), Query Frame = 0

Query:  138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEE---YYRTLETAFDMRLAVGVLRIDGGLDISEMFASDE----------KAQMIFARNRTTSELSEQSLNF--------EDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            LT+EV P V+HYR+ LS    H E  RPTL+EL  E ++ +    + E      ++  +GK IKFGW +GV+MRCLLNIWGVMLFLRL+WVIGQAG+ EG  II L+NIVT++T+ISMSAV TNGQIK GGIYYMISRSLGPEFGGAIG+MFTLANS+AVSMYI+GFCESL D+LR + T    I+D     ND+R++G ++L+ I  LAIVGM+WVTR QM LL +L+ SQ+DF+IGS + P  DEEI KGFVGY+     SNLY  Y   E  GV H+FFSVF+VFFPAVTGIVAGANLSGDLKDP+ AIP GTLLAIV T+++Y+ YG M+  C +R+ASGNV E       +    GE  N    + +C  R C YG+    Q ME+ S+WGPLIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I  FS+G GANNDPVRGYILVF+IS  CILI  LN ++ LLSNFF+AAYALINFSVFHASI+ SPGWRP+FKYYN W+SL+GT+LCI VMFLM +  A+ T V +I LY+++ +RKP+VNWGSS+Q+ S+ +ALKA+  L RV++HVKN+RP+++VL+G P  RP L+DFA+L+TK LSLL    I +A+   +  +AL   A +WL  + +K+F  IT +DS   G +  ++I GLGKL PNM+LMG+K +W+    D EE   Y+  +  A D  LAVG+LR+  GLD S +   +E           A+    RN+++++L  Q +N          D     M+ D  +AS + L         S   R   KT+ S     +   G P+ +  +  ITQF+ K  + G ID++WLYDDGGLT+L+P+IL+TR++F+  +LRIF L+   D+LD D RN+ ALLSKFRI++SDV+++ D+ KKA   TR +F+  I     K G  S +S  S             +TE EL    E+TN  +RI E++ Q S E+  +VM LP+P+++ +  PLYMAWL+  T+ MPP L++RGNQ SV+T
Sbjct:   68 LTREVPPRVEHYRH-LSI---HHEY-RPTLEEL-HERIIPEKRERNVEAE---PQEQKKGKVIKFGWLEGVFMRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRSFGTT---IIDGAS--NDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNLYPDYRNYE--GVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTE-------YLQVKGEP-NEWTIFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRT--CDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDP-VASDSLLESSTTVTVTSKKSRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTS---------NKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGMPPCLIIRGNQTSVIT 1069          

HSP 2 Score: 881.322 bits (2276), Expect = 0.000e+0
Identity = 485/1082 (44.82%), Postives = 685/1082 (63.31%), Query Frame = 0

Query: 1081 EEELQRHSEQTNFQLRIAEI-VRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLTSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQF-DGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLL---DREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLY-PKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDIS--------------DISGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPK----------------SVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS------GDEQGV----QRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            ++ELQ   + +  + R+A++  + +  E D   +T P  +        Y + L   TR++PP +    +       RPTL+EL  R     I P +   N       Q K  V K      FGW++GV++ CLLNIWGVMLFLRL+WV+GQAG+    AII +AN+VT VT+ISMSAVCTNGQIKGGGIY+MISRSLGPEFGGA+GLMFT A S+AVSMYI+GFCE+L D++  F +   DG        +ND+R++G  TL+ +  ++ +GM  V + Q+ LL VL+ASQ+DF+IG+F+ P  DE+   G VG++ +   SNL+PD+ +N +G     F VF+VFFPAVTG+VAGAN+SGDLKDP SAIP GTL AI+ T+ SY+ Y  ++    +R ASGN  E L    +  +   F NC++R C YG   + Q ++L+S WGPLIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P I  F +G G + +P+RGY+L+F I+  CILI  LN ++ LLSNFF+A YA+IN+SVFHAS + SPGWRP+FKY+N W+SL+GT LCI VM LM    AL T + +I LY++V  R P+VNWGSS+Q+ S+ +ALK++  LN VE+HVKN+RP+++V+TG P  RP L DFA LITK  SLL   H++K     R+ + +    + WL  + +KAF+ +T +DSF  G +  +++SGLGKL  NM+++G++  W    P+  ++Y+ V+  + D +L+VG+LRVQ GLD S              D   A EK+    S     S +   S+L+ AL+ D   P                 +V ++SK   S  T  T+ ++   Y    G P+ + I+  +TQF+ K  K G IDV+WLYDDGGLTLL+PYILSTR +F+ C++R+F L+++ D L  +   +A+LL KFRI++ DV+I+SD+ K     T   F+T +       GD         +  ++E  LL   ++T  H+RI E+  + S  S  +VM LP+PR++ +   LY+AWL+ +++ MPP L +RGNQ SV+T YS
Sbjct:   16 QQELQDIDQNSPNRFRVAKVDAKDDEKETD--SLTSPNLQHSYGCDSRYKS-LGQLTREVPPRVEHYRHLSIHHEYRPTLEELHER-----IIPEKRERNVEAEPQEQKKGKVIK------FGWLEGVFMRCLLNIWGVMLFLRLSWVIGQAGIGEGTAIICLANIVTLVTSISMSAVCTNGQIKGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYIVGFCESLQDLLRSFGTTIIDG-------ASNDIRVVGLVTLIGIFILAIVGMDWVTRTQMCLLFVLIASQIDFVIGSFLGPEDDEEIVKGFVGYNVETFVSNLYPDY-RNYEGVDHDFFSVFSVFFPAVTGIVAGANLSGDLKDPSSAIPIGTLLAIVITFLSYVGYGFMMGGCVLRTASGNVTEYLQVKGEPNEWTIFSNCTDRPCMYGLHNDNQAMELASSWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPLIGFFSKGHGANNDPVRGYILVFVISFGCILIAQLNVIAPLLSNFFLAAYALINFSVFHASISKSPGWRPAFKYYNAWVSLIGTLLCIAVMFLMSWVMALFTFVVVITLYLYVSYRKPEVNWGSSTQAHSYSSALKAMLELNRVEEHVKNFRPQVLVLTGMPGSRPPLVDFAYLITKGLSLLVCAHILKATHSQRVHEALTKKAYQWLIRHKVKAFFCITEDDSFEAGCQVLMQISGLGKLRPNMILMGYKGNWRTCDPEELKQYFNVIHNALDRYLAVGILRVQEGLDYSGIIQDEEFVPISTLDKKDAEEKMRRNQSSNQLVSQDVNISVLSDALNSDTESPSMNCDPVASDSLLESSTTVTVTSKK--SRKTLKTQKSNIAMYVSPGGEPLPKSILNNITQFQRKQPK-GLIDVWWLYDDGGLTLLIPYILSTRSQFSGCQLRIFSLASKGDDLDNDQRNLAALLSKFRIDYSDVVIISDIHKKAMDSTRKEFETFIEPFRVKDGDSSDTSKTSNKTCITESELLALKEKTNRHMRIRELLLQYSMESTFIVMALPIPRKDVVSAPLYMAWLELLTKGMPPCLIIRGNQTSVITVYS 1072          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1215275570|gb|OXA61879.1| (Bumetanide-sensitive sodium-(potassium)-chloride cotransporter [Folsomia candida])

HSP 1 Score: 1008.82 bits (2607), Expect = 0.000e+0
Identity = 529/1048 (50.48%), Postives = 713/1048 (68.03%), Query Frame = 0

Query:  138 LTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIW---------GVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYN----AYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQ-----AYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDW-QQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATL-----TKRKLGRDGSVLGRF------QKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            LT+E LP +D+YR+ LS         RPTLDEL + +        D E G     +  +GK IKFGW +GV MRC+LNIW         GVMLFLRL+W +GQAG+LEG  +I+L+N+VT IT  SMSAVSTNGQIK GGIYYMISRSLGPEFG AIG+MFT+ANSIAVS+YI+GFCESL D+L+    ++  ++D     ND+R+IG   LV IL LA VGM+WVTR QM LL++L+ +QV+FIIGS I P  D E AKGF GY+     +N ++    AY +D T   + +F++VF VFFPAVTGIVAGANLSGDLKDPA AIPKGTLLAI  TY++Y+ Y +M+  C++R ASGN+ E       +N TV   + + Q     AYD+C  R C YG+  S Q+ME+IS++G LIYAGCFAATLSSAIASLVGAPRV QA+ KDKL+P IH F++G+GANNDPVRGY LVF+ISL C+LI +LN ++ LLSNFF+ AY LINFSVFHASI+ SPGWRP+FKYYN W+SLLG +LCI VMFL+++ TA+ T  C++ LY+++ +RKP+VNWGSS+Q+QSF +ALK++Q L  V++HVKNYRP+I+V++G P +RP L+DFA+L+ K LSL+    + +     K   AL   A  WL  +  K F+++  ++S   GA++ ++I GLGKL PNMVLMG+K DW + +  D +EY+  +  A DM LAVG+LR   GLD S +   +E+ Q+     +   E+ ++     + SES++S D   +  ++       +RK   D  V  +       +K  K S+S       G  +    +  ITQF+ K  + GTID++WLYDDGGL++LLP++LTTR ++A   LRIF L+   DEL  + RNM ALLSKFRI++SDVI++ D+TKKA+ KT+ +F+++I            +  KS+     D  +   +TE EL  H E+TN  LR+ E+++ +S ++  VVMTLP+P++  +  PLYMAWL+  TR MPPFL++RGNQ SVLT
Sbjct:   74 LTREALPALDNYRDLLSVHAA----PRPTLDELHNATYTEKTKAMDEETG-----EVLKGKVIKFGWLEGVLMRCMLNIWQVNANFENEGVMLFLRLSWCVGQAGILEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTIANSIAVSLYIVGFCESLNDLLKA--NNWGQLIDGGS--NDIRVIGVGVLVAILVLAFVGMDWVTRTQMFLLVVLIAAQVNFIIGSFIGPVDDLEKAKGFPGYNAETFVTNFWSDYRTAYGEDTT---KQDFWTVFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYLSYIGYAVMMGGCAMREASGNLTE-------YNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVI--EEEGQLT---PQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPDQVVNNQLVGFSSNKKSRKHSLSAMYKGPGGAILPRHVLSNITQFQRKQ-KKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVI------------AEFKSD-----DNQDGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFVVMTLPMPRKGTVSAPLYMAWLEMLTRDMPPFLLLRGNQTSVLT 1075          

HSP 2 Score: 887.1 bits (2291), Expect = 0.000e+0
Identity = 462/1005 (45.97%), Postives = 655/1005 (65.17%), Query Frame = 0

Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIW---------GVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIY--QFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNV--DGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGN---------PEELLLDREDELYFQNCSNRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHALSLD-------------EFQPKSVQLSSKNVDSFGTSSTRNNSEVS----YGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV----SGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            RPTLDEL     + + Y  + ++    T +      +K       FGW++GV + C+LNIW         GVMLFLRL+W VGQAG+L  F +I++ANLVT +TA SMSAV TNGQIKGGGIY+MISRSLGPEFG A+GLMFT A SIAVS+YI+GFCE+L D++    +    DG        +ND+R++G   LV +L ++F+GM  V + Q+ LL+VL+A+QV+F+IG+F+ P D+ ++A G  G++ +   +N W D++     D  +   + VF VFFPAVTG+VAGAN+SGDLKDP SAIPKGTL AI  TY SY+ YA+++    MR ASGN          E  +L  E  + + NC+NR+C YG   + Q+++L S +G LIYAGCFAATLSSA+ASLVG PRVFQA+ KDKL+P I  F +G+G + +P+RGY L+F I+L C+LI +LN ++ LLSNFF+  Y +IN+SVFHAS ++SPGWRP+FKY+N W+SLLG  LCI VM L++  TALAT+ C++ LY++V  R P+VNWGSS+Q+QSF +ALKS+Q LN+VE+HVKNYRP+++V+TG P +RP L DFA LI K  SL+   HV+K     ++   +    + WL  +  K FY L  ++SF  G ++ +++ GLGKL  NM+++G++  W    +   +EY+ V+  + DMHL+VG+LR   GLD S++     ++     Q      E   +     LS D             + + K++     N    G SS + + + S    Y    G  +   ++  +TQF+ K QK GTIDV+WLYDDGGL++LLPY+L+TRK++A C +R+F L++R D L  E   MA+LL KFRI++ DVI++ D+TK  + +T   F++++    S D Q      ++E  L+ H ++T  HLR+ E+ + +S  S  VVMTLP+PR+  +   LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct:   96 RPTLDEL-----HNATYTEKTKAMDEETGEVLKGKVIK-------FGWLEGVLMRCMLNIWQVNANFENEGVMLFLRLSWCVGQAGILEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTIANSIAVSLYIVGFCESLNDLLKANNWGQLIDG-------GSNDIRVIGVGVLVAILVLAFVGMDWVTRTQMFLLVVLIAAQVNFIIGSFIGPVDDLEKAKGFPGYNAETFVTNFWSDYRTAYGEDTTKQDFWTVFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYLSYIGYAVMMGGCAMREASGNLTEYNLTVYSEMTVLQDETFIAYDNCTNRDCEYGLYNSAQVMELISSFGGLIYAGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYCLVFVISLGCLLIAELNAIAPLLSNFFLMAYTLINFSVFHASISHSPGWRPAFKYYNAWVSLLGCVLCIAVMFLLNWPTALATVACVLSLYLYVSYRKPEVNWGSSTQAQSFNSALKSIQDLNYVEEHVKNYRPQILVMTGMPGNRPPLIDFAYLIVKNLSLMVCGHVVKGHYSQKVKAALARQAYSWLLRHKKKGFYTLVEDESFEHGAKSIMQICGLGKLRPNMVLMGYKADWVKGNRQDLKEYFNVIHEALDMHLAVGILRAPEGLDYSNVIEEEGQLTPQQLQEIKKEKEIPRNQSESQLSQDGTSSESSSPPGSPKVERKTLPDQVVNNQLVGFSSNKKSRKHSLSAMYKGPGGAILPRHVLSNITQFQRK-QKKGTIDVWWLYDDGGLSILLPYLLTTRKQYAGCTLRIFSLTSRKDELGREQRNMAALLSKFRIDYSDVIVIPDITKKAEDKTKREFESVIAEFKSDDNQ--DGTSITESELMSHREKTNRHLRLRELLQLHSKDSTFVVMTLPMPRKGTVSAPLYMAWLEMLTRDMPPFLLLRGNQTSVLTFYS 1078          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1003342407|gb|AMO44056.1| (Na+/K+/2Cl- cotransporter protein [Marsupenaeus japonicus])

HSP 1 Score: 997.268 bits (2577), Expect = 0.000e+0
Identity = 531/1041 (51.01%), Postives = 710/1041 (68.20%), Query Frame = 0

Query:  132 KPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNV---EEVHFGTDLFNDTVGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLN--------FEDGSESKMSVDSG-----LASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            K L+HYLT+E LP   HYRN  S   G     RPTLD+L + +         A+        +  GK IKFGW +GV MRCLLNIWGVMLFLR++W++GQAGLL  L II ++NIVTV+TT+SMSAV+TNG+IK GG+YYMISRSLGPEFGG+IG+MFTLANSIA + YIIGFC+SL D+L+ Y  +   IVD    IND R++G+I+L+ +LALAIVGM+WVTRVQMGLL LL+ SQ+DF+IG  + P  + E ++GFVG  + V   N  + +   +  GV + FF VF VFFPAVTGIVAGANLSGDLKDP  AIPKGTLLAI  T+VTY++Y  M+A   +R ASGNV   E     T+L  + V    + T    D  G  C +G+  S Q+ME++SAWGPLIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+PGI+SFS G+GANNDPVRGYILVF+IS ICI+IGDLN VS+LLSNFF+AAY+LINFS FHAS+  SPGWRPSF YYN W+SL+G ILC+ VMFL+D+KTA+IT + I+ LY+F+ +RKP VNWGSS+Q+Q++++ALK+   L  V++HVKNYRP+++VL+G P  RP L+DFA  +TK +SLL    + +     +  ++L   A  WL  + I++F+SI   ++L EG+R   ++ GLGKL PN+VL+G+K +W +    +   Y+ TL  A DM   V +LR+  GLD S++   DE   ++   N TT     + +          +DG+ S+ S   G      A  A +T    G +         K ++S++       G  + ++ +  IT FR K  + GTID++WLYDDGGLT+LLP+ILTTR++++   LR+F L+   DELD + R+M  LL+KFRI++SDVI++ D+ KKA + +R +F  +I     KS         +  +DK +  E + ++E EL    E+TN  +R+ E++ +NS EA LVVMTLP+P++  +  PLYMAWL+  TR MPPFL++RGNQ SVLT
Sbjct:   49 KSLRHYLTREALPKESHYRNLNSIVDGG--NVRPTLDDLHNNTFREQQQSTGADPEAQAT--NLRGKVIKFGWIEGVLMRCLLNIWGVMLFLRVSWMVGQAGLLYSLMIIIMANIVTVVTTLSMSAVATNGRIKGGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLQDLLKYYFNNAQ-IVDGA--INDTRIVGTITLIAVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVIGFFMGPLDNLERSQGFVGMSSGVFSHNFASDFRYFD--GVHYEFFGVFGVFFPAVTGIVAGANLSGDLKDPGSAIPKGTLLAIGITFVTYIVYPFMLAGSVVRDASGNVTMFEAYVNSTELLENPVFTNESCTT---DGGGGTCEFGLQNSFQVMELVSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIYSFSRGYGANNDPVRGYILVFVISFICIMIGDLNLVSTLLSNFFLAAYSLINFSCFHASLIKSPGWRPSFTYYNSWVSLVGGILCLIVMFLIDWKTALITFIVILTLYLFVAYRKPDVNWGSSTQAQTYVSALKSTLDLITVEEHVKNYRPQLLVLTGPPGTRPPLLDFAQSITKNISLLACGHVIQGPQTQRVRNSLTRRAYNWLTRHKIRAFYSIVEANNLEEGSRNLFQLVGLGKLRPNIVLLGYKSNWAKCDRLELRAYFNTLHEALDMYYGVAILRVPQGLDYSQI-VEDEDTPVVMNGNETTGAAVAEEIKRNQSAGQLSQDGTGSEASTPPGSPQAERAGTAAVTTEPSGVEA-------LKRRASLANLYRGPGGVELGKDVLNNITTFRRKQ-KKGTIDVWWLYDDGGLTLLLPYILTTRSQWSNCTLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKAKS---------NVEIDKEN--EGLWISEAELLGQREKTNRHVRLRELLLENSREASLVVMTLPMPRKNSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLT 1057          

HSP 2 Score: 848.581 bits (2191), Expect = 0.000e+0
Identity = 465/1000 (46.50%), Postives = 644/1000 (64.40%), Query Frame = 0

Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMI-YQFSSQ--FDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDE-KRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPE--ELLLDREDELYFQNCSNRNCT---------YGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEK-VMTAASQASGASDEDRFSMLNHALSLDEF-------------QPKSVQLSSKNVDSFGTSSTRNNSEVS--YGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGDEQGVQRPI--------VSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            RPTLD+L     N +         QS  AD +A+ T  +     +FGWI+GV + CLLNIWGVMLFLR++W+VGQAGLL S  II +AN+VT VT +SMSAV TNG+IKGGG+Y+MISRSLGPEFGG++GLMFT A SIA + YIIGFC++L D++ Y F++    DG +       ND RI+G+ TL+ +L ++ +GM  V ++Q+ LL +L+ SQ+DF+IG F+ P D  +R+ G VG    +   N   DF +  DG     F VF VFFPAVTG+VAGAN+SGDLKDPGSAIPKGTL AI  T+ +Y++Y  ++A S +R ASGN    E  ++  + L     +N +CT         +G   + Q+++L S WGPLIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P I SF RG+G + +P+RGY+L+F I+  CI+IGDLN VS+LLSNFF+A Y++IN+S FHAS   SPGWRPSF Y+N W+SL+G  LC++VM L+D  TAL T I I+ LY+FV  R PDVNWGSS+Q+Q++++ALKS   L  VE+HVKNYRP+L+V+TG P  RP L DFA  ITK  SLL   HVI+     R+ +++    ++WL  + I+AFY++   ++   G R   +L GLGKL  N++++G++  W    +     Y+  L  + DM+  V +LRV  GLD S I    +  V+   ++ +GA+  +       A  L +              Q +    ++   +  G  + +  + ++  Y    G  + + ++  +T FR K QK GTIDV+WLYDDGGLTLLLPYIL+TR +++ C +RVF L+NR D L  E   MA+LL KFRI++ DVI++ DV K   + +   F  ++   +      I        +SE  LL   ++T  H+R+ E+  ENS  ++LVVMTLP+PR+N +   LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct:   79 RPTLDDL----HNNTFR----EQQQSTGADPEAQATNLRGK-VIKFGWIEGVLMRCLLNIWGVMLFLRVSWMVGQAGLLYSLMIIIMANIVTVVTTLSMSAVATNGRIKGGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLQDLLKYYFNNAQIVDGAI-------NDTRIVGTITLIAVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVIGFFMGPLDNLERSQGFVGMSSGVFSHNFASDF-RYFDGVHYEFFGVFGVFFPAVTGIVAGANLSGDLKDPGSAIPKGTLLAIGITFVTYIVYPFMLAGSVVRDASGNVTMFEAYVNSTELLENPVFTNESCTTDGGGGTCEFGLQNSFQVMELVSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIYSFSRGYGANNDPVRGYILVFVISFICIMIGDLNLVSTLLSNFFLAAYSLINFSCFHASLIKSPGWRPSFTYYNSWVSLVGGILCLIVMFLIDWKTALITFIVILTLYLFVAYRKPDVNWGSSTQAQTYVSALKSTLDLITVEEHVKNYRPQLLVLTGPPGTRPPLLDFAQSITKNISLLACGHVIQGPQTQRVRNSLTRRAYNWLTRHKIRAFYSIVEANNLEEGSRNLFQLVGLGKLRPNIVLLGYKSNWAKCDRLELRAYFNTLHEALDMYYGVAILRVPQGLDYSQIVEDEDTPVVMNGNETTGAAVAEEIKRNQSAGQLSQDGTGSEASTPPGSPQAERAGTAAVTTEPSGVEALKRRASLANLYRGPGGVELGKDVLNNITTFRRK-QKKGTIDVWWLYDDGGLTLLLPYILTTRSQWSNCTLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKAKSNVEIDKENEGLWISEAELLGQREKTNRHVRLRELLLENSREASLVVMTLPMPRKNSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLTFYS 1060          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1061481000|gb|ODM98387.1| (Bumetanide-sensitive sodium-(potassium)-chloride cotransporter [Orchesella cincta])

HSP 1 Score: 989.564 bits (2557), Expect = 0.000e+0
Identity = 514/982 (52.34%), Postives = 686/982 (69.86%), Query Frame = 0

Query:  196 EGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSG-VQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNITQAYDDCEG--RKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKA--QMIF-----------ARNRTTSELSEQSLNFEDGSESKMSVDSGL-----ASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            +GK +KFGW +GV MRC+LNIWGVMLFLRL+WVIG AG+LEG  +I+L+N+VT IT  SMSAVSTNGQIK GGIYYMISRSLGPEFG AIG+MFT+ANSIAVSMYI+GFCESL D+L+    D   I+D     ND+R++G   L+ IL LA VGM+WVTR QM LL++L+ +QVDFI+GS I P  DEE AKGF+ Y+ A   +N +  Y  D  +G  Q +FFS F VFFPAVTGIVAGANLSGDLKDPA AIPKGTLLAI  TY+TY++Y +M+  C++R A+GN  E  +  ++ N T  E       Y DC+   + C+YG+L S Q+ME+IS+WGPLIY GCFAATLSSAIASLVGAPRV QA+ KDKL+P IH F++G+GANNDPVRGYILVFIISL C+LI +LN ++ LLSNFF+ AY LINFS FHA++T SPGWRP+FKYYN W+SL+G +LCI VMFLM + TA+IT  C+I LY+++ +RKP+VNWGSS+Q+QS+ +ALK++Q L  V++HVKNYRP+++VLSG P  RP L+DFA+L+ K LSL+   Q+ + S + K  +AL   A  WL  +  K F+++  ++S   G R+ ++I GLGKL PN VLMG+K DWQ+ +  + +EY+  +  A DM L V +LR+  GLD + +   +++A  Q I             RN++ S+LS      +DG+ S+ S          ++PA  T        + +G  +++ K S+S      +G  +  + +  +TQF+ K  + GTID++WLYDDGGLT+LLP+ILT+R++F+   LRIF L+   DEL  + RNM ALLSKFRI++SDVI++ D+TKKA + T+ +F A+I            +  KS   D     +   VTE EL    E+TN  LR+ E++  +S E+  +VMTLP+P++  +  PLYMAWL+  T+ MPPFLM+RGNQ SVLT
Sbjct:   44 KGKVVKFGWLEGVLMRCILNIWGVMLFLRLSWVIGHAGVLEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTVANSIAVSMYIVGFCESLNDLLKA--NDLT-IIDGGS--NDIRVVGIGVLIAILVLAFVGMDWVTRTQMFLLVVLIAAQVDFIVGSFIGPVDDEERAKGFIEYNAATFATNFWADYRTDYGAGDTQQSFFSAFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYITYILYAVMMGGCALREATGN--ETQYILNM-NATFMEENPDFLLYTDCKDGLQTCDYGMLNSAQVMELISSWGPLIYMGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYILVFIISLGCLLIAELNAIAPLLSNFFLMAYFLINFSCFHATVTKSPGWRPAFKYYNAWVSLVGCVLCIAVMFLMSWVTALITVACVIFLYLYVAYRKPEVNWGSSTQAQSYNSALKSIQDLNVVEEHVKNYRPQLLVLSGMPSSRPPLIDFANLIVKNLSLMLCAQVVKGSYNQKVRNALSRQAYNWLAKHKKKGFYTMIEDESFERGCRSIMQICGLGKLRPNTVLMGYKTDWQRGNRQELKEYFNVIHEALDMYLGVAILRVQEGLDYTGVIEDEDQATPQQILLDMKKDKEKGIVRNQSESQLS------QDGTSSEASSPPSSPKIERSNPAGPTGE------TAVGLKKRQRKHSLSALYAGPNGSALPRDVISHLTQFQRKQ-KKGTIDVWWLYDDGGLTLLLPYILTSRSQFSGCALRIFSLASKKDELGREQRNMAALLSKFRIDYSDVIVIPDVTKKAHENTKKEFDAVI------------APFKSNNSD-----DGTSVTEAELIACREKTNRHLRLRELLLTHSKESTFIVMTLPMPRKGTVSAPLYMAWLEMMTKGMPPFLMIRGNQTSVLT 987          

HSP 2 Score: 861.677 bits (2225), Expect = 0.000e+0
Identity = 443/967 (45.81%), Postives = 633/967 (65.46%), Query Frame = 0

Query: 1191 TVKKDTGPA------RFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPP-SDEKRAMGIVGFDPDLLRSNLWPDFQKNVDG--EQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELLL--------DREDELYFQNCSN--RNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFG-EEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGASDEDRFSMLNHA---LSLDEFQ------------PKSVQLSSKNVDSFGTSSTRNNSEVS--YGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVS-----GDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            T   + GPA      +FGW++GV + C+LNIWGVMLFLRL+WV+G AG+L  F +I++ANLVT +TA SMSAV TNGQIKGGGIY+MISRSLGPEFG A+GLMFT A SIAVSMYI+GFCE+L D++   ++    I G S    ND+R++G   L+ +L ++F+GM  V + Q+ LL+VL+A+QVDF++G+F+ P  DE+RA G + ++     +N W D++ +      Q   F  F VFFPAVTG+VAGAN+SGDLKDP SAIPKGTL AI  TY +Y++YA+++    +R A+GN  + +L        +  D L + +C +  + C YG L + Q+++L S WGPLIY GCFAATLSSA+ASLVG PRVFQA+ KDKL+P I  F +G+G + +P+RGY+L+F I+L C+LI +LN ++ LLSNFF+  Y +IN+S FHA+   SPGWRP+FKY+N W+SL+G  LCI VM LM   TAL T+ C+I LY++V  R P+VNWGSS+Q+QS+ +ALKS+Q LN VE+HVKNYRP+L+V++G PS RP L DFANLI K  SL+    V+K     ++ + +    ++WL  +  K FY +  ++SF  G R+ +++ GLGKL  N +++G++  W    +   +EY+ V+  + DM+L V +LRVQ GLD + +    ++            D+++  + N +   LS D                +S         + G    +    +S  Y   +G+ +   ++  +TQF+ K QK GTIDV+WLYDDGGLTLLLPYIL++R +F+ C +R+F L+++ D L  E   MA+LL KFRI++ DVI++ DVTK   + T   F  +++       + G     V+E  L+   ++T  HLR+ E+   +S  S  +VMTLP+PR+  +   LY+AWL+ +++ MPPFL +RGNQ SVLTFYS
Sbjct:   34 TEDDEDGPALKGKVVKFGWLEGVLMRCILNIWGVMLFLRLSWVIGHAGVLEGFLLISLANLVTFITAFSMSAVSTNGQIKGGGIYYMISRSLGPEFGAAIGLMFTVANSIAVSMYIVGFCESLNDLLK--ANDLTIIDGGS----NDIRVVGIGVLIAILVLAFVGMDWVTRTQMFLLVVLIAAQVDFIVGSFIGPVDDEERAKGFIEYNAATFATNFWADYRTDYGAGDTQQSFFSAFGVFFPAVTGIVAGANLSGDLKDPASAIPKGTLLAITITYITYILYAVMMGGCALREATGNETQYILNMNATFMEENPDFLLYTDCKDGLQTCDYGMLNSAQVMELISSWGPLIYMGCFAATLSSAIASLVGAPRVFQALCKDKLYPVIHFFAKGYGANNDPVRGYILVFIISLGCLLIAELNAIAPLLSNFFLMAYFLINFSCFHATVTKSPGWRPAFKYYNAWVSLVGCVLCIAVMFLMSWVTALITVACVIFLYLYVAYRKPEVNWGSSTQAQSYNSALKSIQDLNVVEEHVKNYRPQLLVLSGMPSSRPPLIDFANLIVKNLSLMLCAQVVKGSYNQKVRNALSRQAYNWLAKHKKKGFYTMIEDESFERGCRSIMQICGLGKLRPNTVLMGYKTDWQRGNRQELKEYFNVIHEALDMYLGVAILRVQEGLDYTGVIEDEDQATPQQILLDMKKDKEKGIVRNQSESQLSQDGTSSEASSPPSSPKIERSNPAGPTGETAVGLKKRQRKHSLSALYAGPNGSALPRDVISHLTQFQRK-QKKGTIDVWWLYDDGGLTLLLPYILTSRSQFSGCALRIFSLASKKDELGREQRNMAALLSKFRIDYSDVIVIPDVTKKAHENTKKEFDAVIAPFKSNNSDDGTS---VTEAELIACREKTNRHLRLRELLLTHSKESTFIVMTLPMPRKGTVSAPLYMAWLEMMTKGMPPFLMIRGNQTSVLTFYS 990          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1325281518|ref|XP_023325442.1| (solute carrier family 12 member 2-like [Eurytemora affinis])

HSP 1 Score: 989.178 bits (2556), Expect = 0.000e+0
Identity = 531/1014 (52.37%), Postives = 705/1014 (69.53%), Query Frame = 0

Query:  153 LSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNGAEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDS--VERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVIPSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVG-EILNITQAYDDC--------EGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQ-ITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELSEQSLNFEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            +SF +   +  RPT++EL DE           + G    E   +GK IKFGW +GVYMRCLLNIWGVMLFLRLTWVIGQ G+++GL ++T +N+VT++T ISMSAV+TNGQIK GGIY+MISR+LGPEFGGAIG+MFTLANSIAVSMYIIGF ESL+DM + Y+  ++GIVD+    R+NDVR+IG ++LVLIL LA VGM+WVTRVQ GLLLLLL SQVDF+IG+ IP SD + +KGF G+    +  N+   Y        + +FFSVFAVFFPAVTGIVAGANLSGDLKDPA AIPKGTLLAI TTY+TY++YG++VA+      SG  EE    T   ND++  E   +   +DDC         G +C +G L  QQ M VIS  G LIY GCFAATLSSAIASLVGAPRVLQA+AKDKL+P I  F+ G+GANNDP+RGYILVF+ISL C+LIG+LN VSSLLSNFFVAAYALINFSVFHASIT SPGWRP+FKYY+ W+SLLGT+LC+ VMF+MD+ TA++T V +I+LY FIY+RKP+ NWGSS+Q+Q F++ALK VQ+LT   DHVKNYRPK++VL+GNP  RP L+DFA+L+TK+LSLL     ITE     K +  ++ +   WLN + IK F+++T + S  EGA+A L + GLGKL+PN+VL+GFK DW  +L    +Y+  L +A+D  L++G+LR+  GLD SE   ++E         R + +  ++    ED  E        +    + T+        V+   +++T++   +     DG P+++  ++ I QF+ +  + G ID++WLYDDGGLT+L+P+IL+TR  +    LR+F L    D+LD DTRNM  LL+KFRI+FSDVI++ D+TK+A   T+++ K ++                       D L    ++E++L  + E+TN  LR+AE++R++S E+  +VMTLP+P++  +   LYMAWL+  TR +PP L++RGNQ SVLT
Sbjct:    1 MSFGRNSGKGQRPTIEELRDEEY-----SKPEQSGAGDVEGQKKGKVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQCGIVQGLLVMTTANLVTLVTAISMSAVATNGQIKGGGIYFMISRALGPEFGGAIGLMFTLANSIAVSMYIIGFAESLLDMFQDYIPGWSGIVDADFENRLNDVRIIGVVTLVLILVLAFVGMDWVTRVQKGLLLLLLCSQVDFLIGTFIPKSDLDHSKGFTGWSLETVTKNVQPHYE-------EQSFFSVFAVFFPAVTGIVAGANLSGDLKDPATAIPKGTLLAIGTTYLTYILYGVVVAFTYSPYGSGVAEEYSIWT---NDSIPLEEKMLVPRWDDCSADATLLRNGTECRFGTLNDQQTMAVISFTGYLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPKIEFFAPGFGANNDPIRGYILVFLISLGCVLIGELNMVSSLLSNFFVAAYALINFSVFHASITKSPGWRPAFKYYSPWVSLLGTLLCVCVMFMMDWVTALLTYVIVIVLYFFIYYRKPEANWGSSAQAQVFVSALKGVQSLTETQDHVKNYRPKVLVLTGNPAHRPPLVDFANLITKKLSLLICAHVITEPG--SKHLALMRESTQAWLNDHKIKGFYTVTESGSWEEGAKACLTLAGLGKLAPNLVLIGFKSDWMTNLEGLHQYFNVLHSAYDHALSIGILRVKNGLDFSEHILAEETVGDHSEERRGSGDEEKEKEKKEDEEEGDGEDKDEIGELQSKTE--------VVQAVRQRTRTISRVTYETHDGTPLDKTIIDSIQQFQGEK-KKGFIDVWWLYDDGGLTLLIPYILSTRKMYTDCSLRVFTLGNKKDDLDRDTRNMCTLLAKFRIDFSDVIVIPDVTKRAQDATKEELKQIL-----------------------DKLPEDCISEDDLLMNKEKTNRHLRLAELLREHSKESQQIVMTLPMPRKGSVCPELYMAWLEIMTRDLPPTLLIRGNQTSVLT 965          

HSP 2 Score: 892.493 bits (2305), Expect = 0.000e+0
Identity = 491/987 (49.75%), Postives = 654/987 (66.26%), Query Frame = 0

Query: 1156 RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGIV-GDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFVPPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEE--------------LLLDREDELYFQNCSNRN---CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWWLYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDISGAHEKVMTAASQASGA---------SDEDRFSMLNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIRDGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMVSGDEQGVQRPIVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            RPT++EL  R    S      +  QSG  D + +    K     +FGWI+GVY+ CLLNIWGVMLFLRLTWV+GQ G++    ++T ANLVT VTAISMSAV TNGQIKGGGIYFMISR+LGPEFGGA+GLMFT A SIAVSMYIIGF E+LLDM   +   + GIV  D E+R NDVRI+G  TLVL+L ++F+GM  V ++Q  LLL+LL SQVDFLIGTF+P SD   + G  G+  + +  N+ P +      E+   F VFAVFFPAVTG+VAGAN+SGDLKDP +AIPKGTL AI TTY +Y++Y +V+A +     SG  EE              +L+ R D+        RN   C +GTL +QQ + + S  G LIY GCFAATLSSA+ASLVG PRV QA+AKDKL+P IE F  GFG + +P+RGY+L+F I+L C+LIG+LN VSSLLSNFFVA YA+IN+SVFHAS   SPGWRP+FKY++ W+SLLGT LC+ VM +MD  TAL T + +I+LY F+  R P+ NWGSS+Q+Q F++ALK +Q+L   +DHVKNYRPK++V+TGNP+ RP L DFANLITK+ SLL   HVI E    + +  M+     WL D+ IK FY +T + S+  G +  + L+GLGKL+ N+++IGF+  W    +   +Y+ VL  ++D  LS+G+LRV+ GLD S+   A E V   + +  G+          +++          + E Q K+  + +          TR  S V+Y   DG P+D+ I+  + QF+ + +K G IDV+WLYDDGGLTLL+PYILSTRK +  C +RVF L N+ D L  +   M +LL KFRI+F DVI++ DVTK  +  T    + ++      +    +SE +LL + ++T  HLR+AE+ +E+S  S  +VMTLP+PR+  +   LY+AWL+ ++R +PP L +RGNQ SVLTFYS
Sbjct:   12 RPTIEEL--RDEEYS------KPEQSGAGDVEGQ----KKGKVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQCGIVQGLLVMTTANLVTLVTAISMSAVATNGQIKGGGIYFMISRALGPEFGGAIGLMFTLANSIAVSMYIIGFAESLLDMFQDYIPGWSGIVDADFENRLNDVRIIGVVTLVLILVLAFVGMDWVTRVQKGLLLLLLCSQVDFLIGTFIPKSDLDHSKGFTGWSLETVTKNVQPHY------EEQSFFSVFAVFFPAVTGIVAGANLSGDLKDPATAIPKGTLLAIGTTYLTYILYGVVVAFTYSPYGSGVAEEYSIWTNDSIPLEEKMLVPRWDDCSADATLLRNGTECRFGTLNDQQTMAVISFTGYLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPKIEFFAPGFGANNDPIRGYILVFLISLGCVLIGELNMVSSLLSNFFVAAYALINFSVFHASITKSPGWRPAFKYYSPWVSLLGTLLCVCVMFMMDWVTALLTYVIVIVLYFFIYYRKPEANWGSSAQAQVFVSALKGVQSLTETQDHVKNYRPKVLVLTGNPAHRPPLVDFANLITKKLSLLICAHVITEP-GSKHLALMRESTQAWLNDHKIKGFYTVTESGSWEEGAKACLTLAGLGKLAPNLVLIGFKSDWMTNLEGLHQYFNVLHSAYDHALSIGILRVKNGLDFSEHILAEETVGDHSEERRGSGDEEKEKEKKEDEEEGDGEDKDEIGELQSKTEVVQAVR------QRTRTISRVTYETHDGTPLDKTIIDSIQQFQGEKKK-GFIDVWWLYDDGGLTLLIPYILSTRKMYTDCSLRVFTLGNKKDDLDRDTRNMCTLLAKFRIDFSDVIVIPDVTKRAQDATKEELKQIL----DKLPEDCISEDDLLMNKEKTNRHLRLAELLREHSKESQQIVMTLPMPRKGSVCPELYMAWLEIMTRDLPPTLLIRGNQTSVLTFYS 968          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1045308339|gb|ANU78813.1| (Na+/K+/2Cl- cotransporter [Portunus trituberculatus])

HSP 1 Score: 986.097 bits (2548), Expect = 0.000e+0
Identity = 515/1036 (49.71%), Postives = 713/1036 (68.82%), Query Frame = 0

Query:  132 KPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNG--AEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDTVGEILNI-TQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQ-SLADAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARN-----RTTSELSEQSLN---FEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            K L+HYLT+E LP   HYRN  S   G     RPTLD+L   +    +  N  ++G++   A  +  GK IKFGW +GVYMRCLLNIWGVMLFLR++WV+GQ+G++  +  + L N+VT +TT+SMSAV+TNG+I+AGG+YYMISRSLGPEFGG+IG+MFTLANSIA + YIIGFC+SL D++  Y  D   IVD    +ND R++G+I+L+ +LALAIVGM+WVTRVQMGLL LL+ SQ+DF++G+ I P +D + ++GF+G    VL  N+   Y   E  G   NFFSVF VFF AVTGIVAGANLSGDLKDPA AIPKGTL AIVTT+ TY+IY +M+    +R A+G+V       +   D      N  T  + D   + C +G+  S Q+ME++SAWGPLIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+PGI  FS+G GANNDPVRGYILVF+IS ICI+IGDLN VS+LLSNFF+A+Y+LINFS FHAS+  SPGWRPSFKYYN WIS LG ILC+ VMFL+D+ TA+ T + I+ LY+F+ +R P VNWGSS+Q+Q++++ALK    L  +++HVKNYRP+I+VL+G    RP L+DFA+ +TK +SLL    + +     +  ++L   +  WL  + I++F+S+    +L +GAR   ++ GLGKL PN V++G+K +W++    + + Y+ TL  A DM   + +LR+  GLD S++   DE + +    N     +TT +   QS      +DG++S+ S   G  SP  + +  +  D +      KK ++S++       G  ++++ +  IT F+ K  + GTID++WLYDDGGLT+L+P+ILTTR++++  KLR+F L+   DELD + R+M  LL+KFRI++SDVI++ D+ KKA + +R +F  +I           +     + VDK +  + +L++E EL    E+TN  +R+ E++ +NS ++ LVVMTLP+P++  +  PLYMAWL+  TR MPPFL++RGNQ SVLT
Sbjct:   48 KSLRHYLTREALPKESHYRNLDSIVDG--SGPRPTLDDLHHNT----LRENQRQQGIDAEAAAANPRGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQSGIVLAILTVILGNVVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLKDLMFYYF-DGAKIVDGA--VNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQNDVQRSQGFIGLSGEVLAKNVGPDYRNFE--GRPQNFFSVFGVFFTAVTGIVAGANLSGDLKDPAEAIPKGTLAAIVTTFCTYIIYPIMIGAAVLRDATGDVNVYQMYQNHSIDENPAFTNCSTTGHVDNGTQVCPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYILVFVISFICIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWLGGILCLIVMFLIDWITALATFLIIVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNTIEEHVKNYRPQILVLTGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLIRHRIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPMELKAYFNTLHEALDMYFGLVILRVPQGLDYSQII-EDEDSPITMNGNEGAITQTTGDKPGQSTANQLTQDGTDSEASSPPG--SPQ-VERSTVATDANT--ENSKKRRTSLANLFRGPGGSELSKDVLNNITMFKRKQ-KKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLI-----------EDFKTKDEVDKEN--DGLLISEAELLGQREKTNRHIRLRELLLENSRDSSLVVMTLPMPRKTSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLT 1052          

HSP 2 Score: 862.448 bits (2227), Expect = 0.000e+0
Identity = 460/1021 (45.05%), Postives = 663/1021 (64.94%), Query Frame = 0

Query: 1134 FTTRKMPPFLMVRGNQDSVLTS---RPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDG--IVGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASG--NPEELLLDR--EDELYFQNCSNRN--------CTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWW-LYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDI----------SGAHEKVMTAASQASGASDEDRFSMLNHALSLDEFQPKSVQLSSKNV--DSFGTSSTRNNSEVSYGIR--DGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV----SGDEQGVQRP--IVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            + TR+  P      N DS++     RPTLD+L   +        E +  Q   A+  A +   K     +FGW++GVY+ CLLNIWGVMLFLR++WVVGQ+G++ +   + + N+VT++T +SMSAV TNG+I+ GG+Y+MISRSLGPEFGG++GLMFT A SIA + YIIGFC++L D+++ +   FDG  IV   +   ND RI+G+ TL+ +L ++ +GM  V ++Q+ LL +L+ SQ+DF++G F+ P +D +R+ G +G   ++L  N+ PD+ +N +G     F VF VFF AVTG+VAGAN+SGDLKDP  AIPKGTLAAI+TT+C+Y++Y ++I A+ +R A+G  N  ++  +   ++   F NCS           C +G   + Q+++L S WGPLIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P I  F +G G + +P+RGY+L+F I+  CI+IGDLN VS+LLSNFF+A+Y++IN+S FHAS   SPGWRPSFKY+N WIS LG  LC++VM L+D  TALAT + I+ LY+FV  RNP+VNWGSS+Q+Q++++ALK+   LN +E+HVKNYRP+++V+TG    RP L DFA  ITK  SLL   HVI+     R+ +++    ++WL  + I+AFY+L    +   G R   +L GLGKL  N +++G++  W    P   + Y+  L  + DM+  + +LRV  GLD S I          +G    +        G S  ++ +  +   S     P S Q+    V  D+   +S +  + ++   R   G+ + + ++  +T F+ K QK GTIDV+WLYDDGGLTLL+PYIL+TR +++ CK+RVF L+NR D L  E   MA+LL KFRI++ DVI++ DV K   + +   F  ++    + DE   +    ++SE  LL   ++T  H+R+ E+  ENS  S+LVVMTLP+PR+  +   LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct:   53 YLTREALPKESHYRNLDSIVDGSGPRPTLDDLHHNTLR------ENQRQQGIDAEAAAANPRGK---VIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQSGIVLAILTVILGNVVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLKDLMFYY---FDGAKIV---DGAVNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQNDVQRSQGFIGLSGEVLAKNVGPDY-RNFEGRPQNFFSVFGVFFTAVTGIVAGANLSGDLKDPAEAIPKGTLAAIVTTFCTYIIYPIMIGAAVLRDATGDVNVYQMYQNHSIDENPAFTNCSTTGHVDNGTQVCPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYILVFVISFICIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWLGGILCLIVMFLIDWITALATFLIIVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNTIEEHVKNYRPQILVLTGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLIRHRIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPMELKAYFNTLHEALDMYFGLVILRVPQGLDYSQIIEDEDSPITMNGNEGAITQTTGDKPGQSTANQLTQ-DGTDSEASSPPGSPQVERSTVATDANTENSKKRRTSLANLFRGPGGSELSKDVLNNITMFKRK-QKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKTKDEVDKENDGLLISEAELLGQREKTNRHIRLRELLLENSRDSSLVVMTLPMPRKTSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLTFYS 1055          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1036124418|gb|ANJ60741.1| (Na+/K+/2Cl- cotransporter [Scylla paramamosain])

HSP 1 Score: 984.941 bits (2545), Expect = 0.000e+0
Identity = 511/1044 (48.95%), Postives = 714/1044 (68.39%), Query Frame = 0

Query:  132 KPLKHYLTKEVLPHVDHYRNRLSFSKGHPERSRPTLDELLDESVLSDINGNDAEKGVNG--AEDSSEGKAIKFGWFDGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLGIITLSNIVTVITTISMSAVSTNGQIKAGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCESLMDMLRQYLTDFNGIVDSVERINDVRLIGSISLVLILALAIVGMEWVTRVQMGLLLLLLVSQVDFIIGSVI-PSSDEEIAKGFVGYDTAVLESNLYNAYTKDETSGVQHNFFSVFAVFFPAVTGIVAGANLSGDLKDPAVAIPKGTLLAIVTTYVTYMIYGLMVAWCSIRSASGNVEEVHFGTDLFNDT---------VGEILNITQAYDDCEGRKCNYGILQSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVLQAVAKDKLFPGIHSFSEGWGANNDPVRGYILVFIISLICILIGDLNQVSSLLSNFFVAAYALINFSVFHASITSSPGWRPSFKYYNKWISLLGTILCIGVMFLMDFKTAIITCVCIILLYVFIYFRKPKVNWGSSSQSQSFITALKAVQALTRVDDHVKNYRPKIMVLSGNPVDRPSLMDFAHLLTKRLSLLESIQITEASLDFKKVDALKSAANEWLNSNHIKSFHSITRNDSLLEGARAALEIGGLGKLSPNMVLMGFKQDWQQSLA-DAEEYYRTLETAFDMRLAVGVLRIDGGLDISEMFASDEKAQMIFARNRTTSELS------EQSLN--FEDGSESKMSVDSGLASPATLTKRKLGRDGSVLGRFQKKTKSSMSLAILDKDGRPVNEETVEKITQFRNKSIRSGTIDIYWLYDDGGLTMLLPHILTTRTKFAKSKLRIFFLSENVDELDEDTRNMGALLSKFRIEFSDVILLQDMTKKADKKTRDDFKAMITIPHPKSGQISQSSLKSEAVDKFDALENVLVTEEELQRHSEQTNFQLRIAEIVRQNSSEADLVVMTLPLPKREQIPYPLYMAWLDFTTRKMPPFLMVRGNQDSVLT 1154
            K L+HYLT+E LP   HYRN  S   G    +RPTLD+L   +    +  N  ++G++   A  +  GK IKFGW +GVYMRCLLNIWGVMLFLR++WV+GQ+G++  L  + L N+VT +TT+SMSAV+TNG+I+AGG+YYMISRSLGPEFGG+IG+MFTLANSIA + YIIGFC+SL D++  Y  D   IVD    +ND R++G+I+L+ +LALAIVGM+WVTRVQMGLL LL+ SQ+DF++G+ I P +D + A+GF+G  + V+  N+   Y + E  G   NFFSVF VFF AVTGIVAGANLSGDLKDPA AIPKGTL AI+TT+ TY+IY +M+    +R A+G+ +      +L  D          +G++ N TQ         C +G+  S Q+ME++SAWGPLIYAGCFAATLSSAIASLVGAPRVLQA+AKDKL+PGI  FS+G GANNDPVRGY+LVF+IS +CI+IGDLN VS+LLSNFF+A+Y+LINFS FHAS+  SPGWRPSFKYYN WIS +G ILC+ VMFL+D+ TA+IT +  + LY+F+ +R P VNWGSS+Q+Q++++ALK    L  +++HVKNYRP+I+VLSG    RP L+DFA+ +TK +SLL    + +     +  ++L   +  WL  + I++F+S+    +L +GAR   ++ GLGKL PN V++G+K +W++    + + Y+ TL  A DM   V +LR+  GLD S++   DE + +    N +    S      + + N   +DG++S+ S     + P +    +        G   KK ++SM+       G  ++++ +  IT F+ K  + GTID++WLYDDGGLT+L+P+ILTTR++++  KLR+F L+   DELD + R+M  LL+KFRI++SDVI++ D+ KKA + +R +F  +I     K           + VDK +  + ++++E E     E+TN  +R+ E++ +NS ++ LVVMTLP+P++  +  PLYMAWL+  TR MPPFL++RGNQ SVLT
Sbjct:   47 KSLRHYLTREALPKESHYRNLDSIVDGS---ARPTLDDLHHNT----LRENQRQQGIDAEAAAANPRGKVIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQSGIILALLTVILGNVVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLQDLMFFYF-DGAKIVDG--EVNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQNDLQRAQGFIGLSSEVMAKNVGPDYREFENRG--QNFFSVFGVFFTAVTGIVAGANLSGDLKDPAEAIPKGTLAAILTTFCTYIIYPIMIGAAVLRDATGDKDVYVMYQNLSIDENPAFTNCSLIGKVDNGTQV--------CPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYVLVFVISFVCIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWVGGILCLIVMFLIDWVTALITFLITVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNSIEEHVKNYRPQILVLSGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLTRHSIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPLELKAYFNTLHEALDMYFGVVILRVPQGLDYSQII-EDEDSPITMNGNESAITQSPEDKPGQSTANQLTQDGTDSEAS-----SPPGSPQVERSAATTDANGENSKKRRTSMANLFRGPGGTELSKDVLNNITMFKRKQ-KKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFRTK-----------DEVDKEN--DGLMISEAEFLGQREKTNRHIRLRELLLENSRDSSLVVMTLPMPRKTSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLT 1050          

HSP 2 Score: 862.448 bits (2227), Expect = 0.000e+0
Identity = 456/1019 (44.75%), Postives = 665/1019 (65.26%), Query Frame = 0

Query: 1134 FTTRKMPPFLMVRGNQDSVL--TSRPTLDELLCRSRNGSIYPCEGRSNQSGTADCQAKDTVKKDTGPARFGWIQGVYVPCLLNIWGVMLFLRLTWVVGQAGLLGSFAIITIANLVTSVTAISMSAVCTNGQIKGGGIYFMISRSLGPEFGGAVGLMFTTAASIAVSMYIIGFCEALLDMIYQFSSQFDGI-VGDSEHRANDVRILGSATLVLLLGISFIGMALVNKIQLLLLLVLLASQVDFLIGTFV-PPSDEKRAMGIVGFDPDLLRSNLWPDFQKNVDGEQLRLFDVFAVFFPAVTGLVAGANMSGDLKDPGSAIPKGTLAAILTTYCSYMMYALVIAASQMRMASGNPEELL----LDREDELYFQNCS--------NRNCTYGTLFNQQMIQLSSMWGPLIYAGCFAATLSSALASLVGGPRVFQAIAKDKLFPYIESFGRGFGRSKEPLRGYLLIFAIALTCILIGDLNRVSSLLSNFFVATYAVINYSVFHASWNNSPGWRPSFKYFNKWISLLGTALCIVVMVLMDLGTALATLICIILLYIFVRTRNPDVNWGSSSQSQSFITALKSLQTLNHVEDHVKNYRPKLIVITGNPSDRPALADFANLITKRSSLLELVHVIKEDVEPRIMDNMKAFVHHWLRDNHIKAFYALTRNDSFLGGVRTAVELSGLGKLSANMLMIGFQERWW-LYPKFGEEYYRVLVLSFDMHLSVGVLRVQGGLDISDI----------SGAHEKVMTAASQASGASDEDRFSM--LNHALSLDEFQPKSVQLSSKNVDSFGTSSTRNNSEVSYGIR--DGNPIDERIVYRMTQFRDKSQKVGTIDVYWLYDDGGLTLLLPYILSTRKKFAKCKMRVFFLSNRPDHLMEEAEKMASLLLKFRIEFEDVIILSDVTKHPKKETMANFQTMV----SGDEQGVQRP--IVSEVNLLKHLDQTRFHLRIAEIAKENSDLSALVVMTLPLPRQNEIPYTLYLAWLDFISRSMPPFLYVRGNQESVLTFYS 2115
            + TR+  P      N DS++  ++RPTLD+L   +        E +  Q   A+  A +   K     +FGW++GVY+ CLLNIWGVMLFLR++WVVGQ+G++ +   + + N+VT++T +SMSAV TNG+I+ GG+Y+MISRSLGPEFGG++GLMFT A SIA + YIIGFC++L D+++ +   FDG  + D E   ND RI+G+ TL+ +L ++ +GM  V ++Q+ LL +L+ SQ+DF++G F+ P +D +RA G +G   +++  N+ PD+++  +  Q   F VF VFF AVTG+VAGAN+SGDLKDP  AIPKGTLAAILTT+C+Y++Y ++I A+ +R A+G+ +  +    L  ++   F NCS         + C +G   + Q+++L S WGPLIYAGCFAATLSSA+ASLVG PRV QA+AKDKL+P I  F +G G + +P+RGY+L+F I+  CI+IGDLN VS+LLSNFF+A+Y++IN+S FHAS   SPGWRPSFKY+N WIS +G  LC++VM L+D  TAL T +  + LY+FV  RNP+VNWGSS+Q+Q++++ALK+   LN +E+HVKNYRP+++V++G    RP L DFA  ITK  SLL   HVI+     R+ +++    ++WL  + I+AFY+L    +   G R   +L GLGKL  N +++G++  W    P   + Y+  L  + DM+  V +LRV  GLD S I          +G    +  +     G S  ++ +    +   S     P+ V+ S+   D+ G +S +  + ++   R   G  + + ++  +T F+ K QK GTIDV+WLYDDGGLTLL+PYIL+TR +++ CK+RVF L+NR D L  E   MA+LL KFRI++ DVI++ DV K   + +   F  ++    + DE   +    ++SE   L   ++T  H+R+ E+  ENS  S+LVVMTLP+PR+  +   LY+AWL+ ++R MPPFL +RGNQ SVLTFYS
Sbjct:   52 YLTREALPKESHYRNLDSIVDGSARPTLDDLHHNTLR------ENQRQQGIDAEAAAANPRGK---VIKFGWLEGVYMRCLLNIWGVMLFLRVSWVVGQSGIILALLTVILGNVVTTLTTLSMSAVATNGRIQAGGVYYMISRSLGPEFGGSIGLMFTLANSIAAATYIIGFCDSLQDLMFFY---FDGAKIVDGE--VNDTRIVGTITLICVLALAIVGMDWVTRVQMGLLFLLIGSQIDFVVGAFIGPQNDLQRAQGFIGLSSEVMAKNVGPDYREFENRGQ-NFFSVFGVFFTAVTGIVAGANLSGDLKDPAEAIPKGTLAAILTTFCTYIIYPIMIGAAVLRDATGDKDVYVMYQNLSIDENPAFTNCSLIGKVDNGTQVCPFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYVLVFVISFVCIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWVGGILCLIVMFLIDWVTALITFLITVALYLFVSYRNPNVNWGSSTQAQTYVSALKTTLDLNSIEEHVKNYRPQILVLSGPVGSRPPLIDFAYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLTRHSIRAFYSLVEGSNLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCDPLELKAYFNTLHEALDMYFGVVILRVPQGLDYSQIIEDEDSPITMNGNESAITQSPEDKPGQSTANQLTQDGTDSEASSPPGSPQ-VERSAATTDANGENSKKRRTSMANLFRGPGGTELSKDVLNNITMFKRK-QKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFRTKDEVDKENDGLMISEAEFLGQREKTNRHIRLRELLLENSRDSSLVVMTLPMPRKTSVSAPLYMAWLETLTRDMPPFLLIRGNQTSVLTFYS 1053