BLAST of hypothetical protein DAPPUDRAFT_329527 vs. L. salmonis genes
Match: EMLSAG00000009984 (supercontig:LSalAtl2s:LSalAtl2s653:17916:32674:1 gene:EMLSAG00000009984 transcript:EMLSAT00000009984 description:"maker-LSalAtl2s653-augustus-gene-0.25")

HSP 1 Score: 1846.63 bits (4782), Expect = 0.000e+0
Identity = 919/1313 (69.99%), Postives = 1085/1313 (82.64%), Query Frame = 0

Query:   44 AGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDET--GSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIP-AIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGG-----LEKD----LDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGA-KGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKK---PWE-DSDATSESD 1339
            A GG  KRLSVERIYQKK+QLEHILLRPD+YIGSV P+TE M++++E   ++VNK+IT+VPGLYKIFDEILVNAADNKQRD  MDTIK+DI+ EKNEI+VFNNGRGIPV  HKDEKMFVPT+IFGHLLTSSNFNDEEEKVTGGRNG+GAKLCNVFS+KFTVETAS+E +K FKQVW  NMSKA E K+  +S G DYTR+TF+PDL KF+M SLD +TVALLSRRAYD+AASSKGVKVFLNGKRI +K FKDYVDFF+K +E+E    S +K ++EA G RWEVA AV +NGF+Q SFVNSIATTKGGRHVD+V+ +I   + E +KKKNKGG++IKP QI+NHLW+F+N LIVNPTFDSQTKENMTL +  FGSKC  + KF   + KSG+VE+VLAWSKFK DQ +K   S KKT+KLKGI KLEDANDAGT+NSIDCTLILTEGDSAKTLA AGL VVGRDKFGVYPLRGKLLNVREATHKQ++ENKEINE+VKI+GLTYKKKY T DD+KSLRYGK+MIMTDQDQDGSHIKGLLINFIHHNWP+LL+ PFLEEFITPIVKA+KG  S  F+SLPE  EWK+ T NW ++KIKYYKGLGTSTSKEAKEYFSDM+RH+ILF ++  QDDH+I MAFSKKA+++RKEWLT WME+CKRRKELG++E+YLYEK+T++V+YS+F+NKELVLFSNLDNERSIPS+VDG KPGQRKV+FTCLKRNDKRE+KVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNN+NLLLPIGQFGTRLAGGKDHAS RYIFTQ++P+TR +F+ +DD VL YL DDN ++EPE+Y+PI+PMVL+NGADGIGTGWMTK+PNYNP++I++N+ ++I+G E ++M PWFK FKG IE I  Q+YVVNGE+A++S+ KIE++ELPV++WTQ+YKE VME MLNGSEK P +II DYKEYHTDVTVKFVV M++DKLR+AE  KGLHQFFKLQTTISTSSMVLFDA GCL+KY N LEI+ EF+ +R+ FYEKRK YL GMLEA++LKL NQARFI  KC+GSLVVENKKRK +I +L  KGFDSDPVK WK  Q+++ D++  +E G     +E+      DYDYL+ MAMWSLT+ERKDELLR+  EK  EL  ++ T ++D+W+ DLKEF  K DE+  KE ED   + + E  KG  KG  K KI    SP GIR+VP I +EL+ K AKAAAAK RK +K     +K  + V DEFD M   KD N  L +K    ++ KG  K  S  S     P+     KGKK   PWE DS +  E D
Sbjct: 1714 AKGGQKKRLSVERIYQKKTQLEHILLRPDSYIGSVQPLTESMYVIEE--GKVVNKDITFVPGLYKIFDEILVNAADNKQRDPKMDTIKVDIDAEKNEIKVFNNGRGIPVVQHKDEKMFVPTMIFGHLLTSSNFNDEEEKVTGGRNGFGAKLCNVFSSKFTVETASKEYKKSFKQVWGANMSKASEPKITPYS-GEDYTRVTFSPDLEKFHMSSLDPNTVALLSRRAYDVAASSKGVKVFLNGKRIPIKGFKDYVDFFIKCYEEENPGASEIKPVFEACGPRWEVAXAVXENGFKQXSFVNSIATTKGGRHVDHVTDLITKALGETIKKKNKGGISIKPFQIKNHLWIFVNALIVNPTFDSQTKENMTLQVKSFGSKCVLSDKFNNLINKSGIVEAVLAWSKFKQDQMLKKNTSGKKTSKLKGIPKLEDANDAGTRNSIDCTLILTEGDSAKTLAXAGLSVVGRDKFGVYPLRGKLLNVREATHKQLLENKEINELVKIMGLTYKKKYETDDDMKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPTLLKRPFLEEFITPIVKASKGQSSFPFYSLPEFEEWKNSTPNWPSFKIKYYKGLGTSTSKEAKEYFSDMRRHRILFSYDGQQDDHSIQMAFSKKAVEERKEWLTNWMEDCKRRKELGMAEVYLYEKNTRSVTYSEFVNKELVLFSNLDNERSIPSLVDGLKPGQRKVVFTCLKRNDKREIKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNVNLLLPIGQFGTRLAGGKDHASARYIFTQLNPMTRYLFHIHDDYVLKYLRDDNLRVEPEYYVPILPMVLINGADGIGTGWMTKVPNYNPKDIIKNIKSLINGGEMKEMVPWFKHFKGSIEYIGEQKYVVNGEVANLSDTKIEISELPVKTWTQTYKESVMEQMLNGSEKNPSSIISDYKEYHTDVTVKFVVTMASDKLREAE-RKGLHQFFKLQTTISTSSMVLFDAYGCLKKYNNPLEIISEFYDVRIRFYEKRKKYLVGMLEADNLKLKNQARFIKMKCEGSLVVENKKRKDIILDLTNKGFDSDPVKCWKKVQEIDCDTEEQTEEGDDTINVEQSDDEKKDYDYLMSMAMWSLTKERKDELLRKAQEKQDELDAVKATLEKDMWKKDLKEFENKYDELMKKEKEDLAKLYAVE--KGVRKGGAKSKISTQASPHGIRIVPRIDDELRTKVAKAAAAKDRKAKKDKNPVVK--DEVPDEFDDMVTGKDGNEKLVDK----ISKKGVVKSSSRKSDSLSSPK-----KGKKRENPWESDSGSGGEED 3009          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|6094494|sp|O16140.1|TOP2_BOMMO (RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II; Short=TOPOII)

HSP 1 Score: 1619.75 bits (4193), Expect = 0.000e+0
Identity = 794/1259 (63.07%), Postives = 971/1259 (77.12%), Query Frame = 0

Query:   34 KANGEAK-KPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVP-----GLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKW-KNAQKME-----EDSDSDSEG---------GLEKDLD-----------YDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLK--IEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRK 1258
            K NG  +  P  G  GA    +E+IYQKKSQLEHILLRPDTYIGSV   TE MW+ D+  + ++ KE+T VP     GLYKI+DEILVNAADNKQRD  MD IKIDIN+E N I V+NNG GIPV MHKDEKM+VPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FSTKFTVETAS++ +K FKQ W +NM+KA E K+KD     D+T+I F+PDL KF ME L+ D V L+SRRAYD+AASS+GVKV+LNG+R+ +  FKDYVD ++KG +DE G P+K +YE   +RWEVA+ +SD GFQQ+SFVNSIATTKGG+HVD V+  +  N+ EVLKKKNKGGV IKP+Q++ H+WLF+NCLIVNPTFDSQTKENMTL    FGSKC+ + KF   + K G+VESVL W+KFKA Q+   K S KK +KLKGI KLEDANDAGTKN+  CTLILTEGDSAKTLAV+GL VVGRD +GV+PL GK LNVR+A+HKQ++EN EIN ++KI+GL YKKKY++VDDLK+LRYGK+MIM DQDQDGSHIKGL+INFIHHNWP LL+LPFLEEFITPIVKATK     SF+SLPE  EWK  T+N HTY IKYYKGLGTSTSKEAKEYF +M RH+I FK+    DDH I +AFSKK  DQRKEWLT  M+E KRRKE+GLSE YLY K+TKTV+YSDF+N ELVLFSN DN RSIPS++DG KPGQRKV+FTC+KRNDKREVKVAQLAGSV E SAYHHGE SL  TI+NLAQN+VGSNNINLL P GQFGTRL+GGKD ASPRYIFT MSPLTRLIF+ +DD +L +  +DNQKIEP +Y+PIIPMVLVNG +GIGTGW TKIPNYNPR+I  NL  ++DG +P+ M PW+K+FKG +E     +YV++GE A +  +KIE+TELPV +WT +YKE V+EPML G++K+  +I +Y+EY+TD TV+FVV +   KL + E + G+H+ FKLQTTIS + M  FD   CL KY+ V EI+REF+ LR+ +Y +RK+YLEG L+AE+ KLSNQARFIL+KCD  LV+ENKKRKA++EEL ++G+  DP+  W K A K++     ED D+             G   D D           ++YLLGM+MW LT+E+KDELL+++D+K  EL  L+  +   LWR DL  F+ KLDEVE KE  +E + ++K++ K   GKK  K   + +PS  G RV P I+++L  +   A  AK+RK
Sbjct:   15 KQNGTGEPAPAKGQKGA----IEKIYQKKSQLEHILLRPDTYIGSVERATETMWVYDKVKECMMQKELTSVPDCSFPGLYKIYDEILVNAADNKQRDPKMDVIKIDINQETNTISVYNNGSGIPVVMHKDEKMYVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTKFTVETASKQYKKHFKQTWGSNMTKASEPKIKDSGKDDDFTKIVFSPDLAKFKMEKLEDDIVLLMSRRAYDVAASSQGVKVYLNGERLKINKFKDYVDLYIKGKDDENGQPLKVVYEKVSDRWEVALTISDMGFQQVSFVNSIATTKGGKHVDTVADSVVKNVLEVLKKKNKGGVNIKPYQVKTHMWLFVNCLIVNPTFDSQTKENMTLQAKSFGSKCNLSEKFITAVTKCGLVESVLTWAKFKA-QDQLVKASGKKQSKLKGIPKLEDANDAGTKNAHLCTLILTEGDSAKTLAVSGLSVVGRDHYGVFPLEGKPLNVRDASHKQVLENVEINNLIKILGLQYKKKYNSVDDLKTLRYGKVMIMADQDQDGSHIKGLIINFIHHNWPELLKLPFLEEFITPIVKATKKDKEISFYSLPEFEEWKKETENHHTYNIKYYKGLGTSTSKEAKEYFQNMDRHRIRFKYSGPTDDHHIELAFSKKGADQRKEWLTNHMDEVKRRKEIGLSERYLYTKETKTVTYSDFVNLELVLFSNGDNVRSIPSMIDGLKPGQRKVIFTCIKRNDKREVKVAQLAGSVAEHSAYHHGEQSLAMTIVNLAQNYVGSNNINLLEPRGQFGTRLSGGKDSASPRYIFTLMSPLTRLIFHPHDDPLLVHEFEDNQKIEPVYYLPIIPMVLVNGVEGIGTGWSTKIPNYNPRDIAENLRRLLDGEKPKVMHPWYKNFKGNVEGF-GDKYVISGEAAILPGDKIEITELPVGTWTHNYKENVLEPML-GTDKVKPLISEYREYNTDTTVRFVVSLLPGKLSEIEAE-GIHKVFKLQTTISMTCMNAFDYNCCLEKYDKVEEILREFYDLRVKYYVRRKDYLEGQLQAEADKLSNQARFILKKCDKGLVIENKKRKAMVEELIKRGYAPDPIADWKKRASKIQGLTALEDDDAQESEEEEPEPDPKGKPVDPDKAFQQLKEVKKFNYLLGMSMWMLTKEKKDELLKQRDQKLTELTTLKNKTAPMLWREDLDAFLIKLDEVEDKERREE-LNVNKKTSKAMAGKKNRKSMFDIIPSENGRRVEPKISDDLIKRIQAAEKAKTRK 1264          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|363548478|sp|Q01320.2|TOP2A_MOUSE (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme)

HSP 1 Score: 1616.28 bits (4184), Expect = 0.000e+0
Identity = 792/1304 (60.74%), Postives = 991/1304 (76.00%), Query Frame = 0

Query:   48 GAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK---------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKL-GVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNS 1341
            G KRLS+ERIYQKK+QLEHILLRPDTYIGSV  VT++MW+ DE+   I  +E+T+VPGLYKIFDEILVNAADNKQRD  M  I++ I+ E N I ++NNG+GIPV  HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETASRE +K FKQ W +NM +A + +LK FS G DYT ITF PDL KF M+SLD D VAL+ RRAYDIA S+K VKVFLNG  + VK F+ YVD +LK   DETG+ +K I+E    RWEV + +S+ GFQQ+SFVNSIAT+KGGRHVDYV+  I S + +V+KKKNKGGV +K HQ++NH+W+F+N LI NPTFDSQTKENMTL    FGS C  + KF       G+VES+L W KFKA  ++  K S+ K  K+KGI KL+DANDAG++NS +CTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y   D LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR  FLEEFITPIVK +K     +F+SLPE  EWKS T N   +K+KYYKGLGTSTSKEAKEYF+DMKRH+I FK+   +DD AI++AFSKK +D RKEWLT +ME+ ++RK LGL E YLY + T  ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMS+YHHGE SLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPL RL+F   DD  L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW  KIPN++ RE+V N+  ++DG EP  M P +K+FKG IE + + +YV+NGE+A +    IE++ELP+R+WTQ+YKEQV+EPMLNG+EK P++I DY+EYHTD TVKFV++M+ +KL +AE   GLH+ FKLQ++++ +SMVLFD  GCL+KY+ VL+I+R+FF LRL +Y  RK +L GML AES KL+NQARFILEK DG +V+ENK +K LI+ L ++G+DSDPVK WK AQ+           +   S S+   E    ++YLL M +W LT+E+KDEL ++++EK QEL  L+  S  DLW+ DL  F+E+L+ VEAKE +DE+V L  ++ K    K ++  + LPSP G RV+P +  E+K +A K    K RK  K+  +N++   A         +D     SL +++      + GAKK+ +    P K      K + K PW DS++   S+ S
Sbjct:   23 GKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDED-VGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFS-GEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAE-RVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEK----KIRK--KIKSENVEGTPA---------EDGAEPGSLRQRIEKKQKKEPGAKKQTTLPFKPVK------KGRKKNPWSDSESDVSSNES 1302          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|6094498|sp|O46374.1|TOP2A_PIG (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme)

HSP 1 Score: 1610.89 bits (4170), Expect = 0.000e+0
Identity = 847/1583 (53.51%), Postives = 1073/1583 (67.78%), Query Frame = 0

Query:   36 NGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK---------------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEEL--KNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSP-----KTKGKKPW---------------------EDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGSEVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKK---PKAKK--PKASPKVKKPPAAATKKSKKTSQGSDYDDSD-------------------EDDMFKDSDLSVLSVEKKVRGGVRRAAAQQKFTYSSDESDF 1551
            N +  K K      KRLS+ERIYQKK+QLEHILLRPDTYIGSV  VT++MW+ DE+   I  +E+T+VPGLYKIFDEILVNAADNKQRD  M  I++ I+ E N I ++NNG+GIPV  HK EKM+VP LIFG LLTSSN++DEE+KVTGGRNGYGAKLCN+FSTKFTVETASRE +K FKQ W +NM +A E +LK F+ G DYT ITF PDL KF M+SLD D VAL+ RRAYDIA S+K VKVFLNG ++ VK F+ YVD +LK   DETG+P+K I+E    RWEV + +S+ GFQQ+SFVNSIAT+KGGRHVDYV+  I + + +V+KKKNKGGV +K HQ++NH+W+F+N LI NPTFDSQTKENMTL +  FGS C  + KF       G+VES+L W KFKA  ++  K S+ K N++KGI KL+DANDAG +NS +CTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y   D LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR  FLEEFITPIVK +K     +F+SLPE  EWKS T N   +K+KYYKGLGTSTSKEAKEYF+DMKRH+I FK+   +DD AI++AFSKK ID RKEWLT +ME+ ++RK LGL E YLY + T  ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMS+YHHGE SLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPL RL+F   DD  L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW  KIPN++ RE+V N+  ++DG EP  M P +K+FKG IE +   +YV++GE+A ++   IE++ELP+R+WTQ+YKEQV+EPMLNG+EK P +I DY+EYHTD TVKFVV+M+ +KL +AE   GLH+ FKLQT+++ +SMVLFD  GCL+KY+ VL+I+R+FF LRL +Y  RK +L GML AES KL+NQARFILEK DG +++ENK +K LI+ L ++G+DSDPVK WK AQ+                 + SDS ++ G      ++YLL M +W LT+E+KDEL + ++EK QEL  L+  S  DLW+ DL  F+E+L+ VEAKE +DE++ L  +  K    K ++  E LPSP G RV+P +  E+  + +       KS   E   +++  EVE +K   +                         KK+  +  T  K+  + P     K K + PW                     E   A +++  +V+ D  +D    ++K++ E    S+ S  + E             +    +  PK +++T P+   FD DD+ ++      PS+P A  P+  +         +  P+ KK  T+ K   K   S      KK   PK  K  P     V K P     K ++  + S  DDSD                    DD   D DL+V S     R    R     K+   SDE D 
Sbjct:   12 NMQVNKTKKNEEAKKRLSIERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDIG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFN-GEDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDLYLKDKVDETGNPLKIIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVAKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRLLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAE-RVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKEADKSDSVADSGPT----FNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFIEELEAVEAKEKQDEQIGLPGKGGKAKGKKTQMA-EVLPSPCGKRVIPRVTVEMKAEAEKKIKKKIKSENTEGSPQEDGMEVEGLKQRLE-------------------------KKQKREPGTKTKKQTTLPFKPIKKAKKRNPWSDSESDISSDESNFNVPPREKEPRRAAAKTKFTVDLDSDEDFSDADEKTRDEDFVPSDTSPQKAET------------SPKHTNKEPKPQKST-PSVSDFDADDAKDNVPP--SPSSPVADFPAVTE---------TIKPVSKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGAKKDPDLDSDVSKKPNPPKPKGRRKRKPSTSDDSDSNFEKMISKAVTSKKPKGESDDFHLDLDLAVAS-----RAKSGRTKKPIKYLEESDEDDL 1532          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|3122959|sp|O42131.1|TOP2B_CHICK (RecName: Full=DNA topoisomerase 2-beta; AltName: Full=DNA topoisomerase II, beta isozyme)

HSP 1 Score: 1608.96 bits (4165), Expect = 0.000e+0
Identity = 788/1315 (59.92%), Postives = 991/1315 (75.36%), Query Frame = 0

Query:   49 AKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK--------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKI----EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMC--------DDKDMNRSLS----EKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESD 1339
            +K++SVER+YQKK+QLEHILLRPDTYIGSV P+T+ MW+ DE+   +  +E+T+VPGLYKIFDEILVNAADNKQRDKNM  IKI I+ E N I ++NNG+GIPV  HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETA +E +  FKQ W NNM K  E K+K F  G DYT ITF PDL KF ME+LD D V+L++RRAYD+A S KGVKV LNGK++ V  F+ YVD ++K   DETG  +K I+E   ERW+V + +S+ GFQQ+SFVNSIATTKGGRHVDYV   +   + EV+KKKNK GV++KP Q++NH+W+F+NCLI NP+FDSQTKENMTL    FGSKC  + KFF      G++ES+L W KFKA  ++  K SS K +K+KGI KL+DANDAG K+S+DCTLILTEGDSAK+LAV+GLGV+GRD++GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y   + LKSLRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLL+  FLEEFITPIVKA+K     SF+S+PE  EWK H +N   +KIKYYKGLGTST+KEAKEYF+DM+RH+ILF++   +DD AI +AFSKK ID RKEWLT +ME+ ++R+  GL E +LY   TK ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMSAYHHGE +LM TI+NLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPL RL+F S DD +L +L DDNQ++EPEWY+PIIPMVLVNGA+GIGTGW  K+PNY+ REIV N+  M+DG +P  M P +K+F+G I+ +   +YVV+GEI  +  N +E+TELPVR+WTQ YKEQV+EPMLNG+EK PA+I DYKEYHTD TVKFVV+M+ +KL QAE   GLH+ FKLQT+++ +SMVLFD  GCL+KYE V +I++EFF LRL++Y  RK +L GML AES KL+NQARFILEK  G + +EN+ ++ LI+ L ++G++SDPVK WK AQ+           + D+ S  G     D++Y+L M++WSLT+E+ +EL++ +D K +EL  L+  S  DLW+ DL  F+E+L++VEA+E ED    +  +  KG  GK K+K     E +PSP G R+VP I   +K  A++    K        KK   +  A+K EFD            D  +N + S     KL     + G + R + SST      S+ K K + PW D ++ SESD
Sbjct:   51 SKKMSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDED-VGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFE-GDDYTCITFQPDLSKFKMENLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEA-AGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQITSAMKADASRKLLKK--------KKGDADSVAIKMEFDEEFGGVQAEGGGDDTVNTAASGTKTPKLKREKKEPGTRVRRAPSST----KSSAKKVKKRNPWSDDESKSESD 1350          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|20141946|sp|Q02880.3|TOP2B_HUMAN (RecName: Full=DNA topoisomerase 2-beta; AltName: Full=DNA topoisomerase II, beta isozyme)

HSP 1 Score: 1608.58 bits (4164), Expect = 0.000e+0
Identity = 800/1339 (59.75%), Postives = 1001/1339 (74.76%), Query Frame = 0

Query:   22 NGEAKKANGEAKKANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK--MEEDS------DSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKES---QKGAKGKKKLKI-EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGK---------KPWEDSDATSESD 1339
            N  AKK   E    N  +KK          LSVER+YQKK+QLEHILLRPDTYIGSV P+T+ MW+ DE+   +  +E+T+VPGLYKIFDEILVNAADNKQRDKNM  IK+ I+ E N I ++NNG+GIPV  HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETA +E +  FKQ W NNM K  EAK+K F  G DYT ITF PDL KF ME LD D VAL++RRAYD+A S +GVKV  NGK++ V  F+ YVD ++K   DETG  +K I+E A ERW+V + +S+ GFQQ+SFVNSIATTKGGRHVDYV   +   + EV+KKKNK GV++KP Q++NH+W+FINCLI NPTFDSQTKENMTL    FGSKC  + KFF      G+VES+L W KFKA  ++  K SS K +K+KGI KL+DANDAG K+S++CTLILTEGDSAK+LAV+GLGV+GRD++GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y   + LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLL+  FLEEFITPIVKA+K     SF+S+PE  EWK H +N   +KIKYYKGLGTST+KEAKEYF+DM+RH+ILF++   +DD AI +AFSKK ID RKEWLT +ME+ ++R+  GL E +LY   TK ++Y+DFINKEL+LFSN DNERSIPS+VDGFKPGQRKV+FTC KRNDKREVKVAQLAGSV EMSAYHHGE +LM TI+NLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT +S L RL+F + DD +L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW  K+PNY+ REIV N+  M+DG +P  M P +K+FKG I+ +   +Y V+GEI  +  N +E+TELPVR+WTQ YKEQV+EPMLNG++K PA+I DYKEYHTD TVKFVV+M+ +KL QAE   GLH+ FKLQTT++ +SMVLFD  GCL+KYE V +I++EFF LRL++Y  RK +L GML AES KL+NQARFILEK  G + +EN+ +K LI+ L ++G++SDPVK WK AQ+   EED       DS S+ G     D++Y+L M++WSLT+E+ +EL++++D K +E+  L+  S  DLW+ DL  F+E+LD+VE++E ED    +S ++   + G    KKL++ E +PSP G R++P I       A KA A  S+K  K  K +L +  AVK EFD       +  +  E L  SV      K   +   P  R + +P + GK          PW D ++ SESD
Sbjct:   29 NNAAKKEESETANKNDSSKK----------LSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDED-VGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFD-GEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEA-AGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEIT------AMKADA--SKKLLKKKKGDL-DTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESD 1345          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|76363529|sp|Q64511.2|TOP2B_MOUSE (RecName: Full=DNA topoisomerase 2-beta; AltName: Full=DNA topoisomerase II, beta isozyme)

HSP 1 Score: 1608.19 bits (4163), Expect = 0.000e+0
Identity = 796/1317 (60.44%), Postives = 996/1317 (75.63%), Query Frame = 0

Query:   49 AKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKW----------KNAQKMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKG------AKGKKKLKI-EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFD--FMCDDKDMN--RSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKK-----PWEDSDATSESD 1339
             K+LSVER+YQKK+QLEHILLRPDTYIGSV P+T+ MW+ DE+   +  +E+T+VPGLYKIFDEILVNAADNKQRDKNM  IK+ I+ E N I ++NNG+GIPV  HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETA +E +  FKQ W NNM K  EAK+K F  G DYT ITF PDL KF ME LD D VAL++RRAYD+A S KGVKV  NGK++ V  F+ YVD ++K   DETG  +K I+E A ERW+V + +S+ GFQQ+SFVNSIATTKGGRHVDYV   + S + EV+KKKNK GV++KP Q++NH+W+FINCLI NPTFDSQTKENMTL    FGSKC  + KFF      G+VES+L W KFKA  ++  K SS K +K+KGI KL+DANDAG K+S++CTLILTEGDSAK+LAV+GLGV+GRD++GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y   + LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLL+  FLEEFITPIVKA+K     SF+S+PE  EWK H +N   +KIKYYKGLGTST+KEAKEYF+DM+RH+ILF++   +DD AI +AFSKK ID RKEWLT +ME+ ++R+  GL E +LY   TK ++Y+DFINKEL+LFSN DNERSIPS+VDGFKPGQRKV+FTC KRNDKREVKVAQLAGSV EMSAYHHGE +LM TI+NLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT +S L RL+F + DD +L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW  K+PNY+ REIV N+  M++G +P  M P +K+FKG I+ +   +Y V+GEI  +  N +E+TELPVR+WTQ YKEQV+EPMLNG++K PA+I DYKEYHTD TVKFVV+M+ +KL QAE   GLH+ FKLQTT++ +SMVLFD  GCL+KYE V +I++EFF LRL++Y  RK +L GML AES KL+NQARFILEK  G + +EN+ +K LI+ L ++G++SDPVK W          +++Q   +DS SDS  G     D++Y+L M++WSLT+E+ +EL++++D K +E+  L+  S  DLW+ DL  F+E+LD+VEA+E ED   IL+  S K           KKL++ E +PSP G R+VP I       A KA A  SRK  K  KK   +   VK EFD  F     +     +L+    V+   K  +++    +  +K P S+ KT  KK     PW D ++ SESD
Sbjct:   34 TKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDED-VGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFD-GEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEA-AGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDS--GTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQERED---ILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEIT------AMKADA--SRKLLK-KKKGDPDTTVVKVEFDEEFSGTPAEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPTSTGKTNAKKVKKRNPWSDDESKSESD 1333          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|1174741|sp|P41515.1|TOP2A_CRIGR (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme)

HSP 1 Score: 1607.81 bits (4162), Expect = 0.000e+0
Identity = 835/1479 (56.46%), Postives = 1053/1479 (71.20%), Query Frame = 0

Query:   50 KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK-------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKK--PWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGSEVSDYEPEAKIAKNMAQNDIFNSSD-----------DSDAPK-------VKRATAPAHKVFDLDDSDEDFDKV-LKPSAPAAK--------RPSSDQ----KSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKK 1488
            KRLS+ERIYQKK+QLEHILLRPDTYIGSV  VT++MW+ DE+   I  +E+T+VPGLYKIFDEILVNAADNKQRD  M  I++ I+ E N I ++NNG+GIPV  HK EKM+VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FST+FTVETAS+E +K FKQ W +NM +A + +LK F+ G DYT ITF PDL KF M+SLD D VAL+ RRAYDIA S+K VKVFLNG ++ VK F+ YVD +LK   DETG+ +K ++E    RWEV + +S+ GFQQ+SFVNSIAT+KGGRHVDYV+  I S + +V+KKKNKGGV +K HQ++NH+W+F+N LI NPTFDSQTKENMTL    FGS C  + KF       G+VES+L W KFKA  ++  K S+ K N++KGI KL+DANDAG++NS +CTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y   D LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR  FLEEFITPIVK +K     +F+SLPE  EWKS T N   +K+KYYKGLGTSTSKEAKEYF+DMKRH+I FK+   +DD AI++AFSKK +D RKEWLT +ME+ ++RK LGL E YLY + T  ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMS+YHHGE SLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPLTRL+F   DD  L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW  KIPN++ RE+V N+  ++DG EP  M P +K+FKG IE + + +YV+NGE+A ++   IE++ELP+R+WTQ+YKEQV+EPMLNG+EK P +I DY+EYHTD TVKFV++M+ +KL +AE   GLH+ FKLQT+++ +SMVLFD  GCL+KY+ VL+I+++FF LRL +Y  RK +L GML AES KL+NQARFILEK DG +++ENK +K LI+ L ++G+DSDPVK WK AQ+        EE  + +S+   E    ++YLL M +W LT+E+KDEL ++++EK QEL  L+  S  DLW+ DL  F+E+L+ VEAKE +DE+V L  +  K    K ++  E LPSP G RV+P +  E+K +A K    K RK  K+  +N   VE    E            SL +++     +K  KK     +  +      P  KGKK  PW D    SESD S      ++S  D    + +P+  +       +AK   ++  ++ F+ SD           D+  PK        K+A  P       D   ++ D V   P  PAA         +PSS Q    K     S  S S    KKR + K  K     +   P +KP   K K S K K+
Sbjct:   25 KRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDED-VGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTRFTVETASKEYKKMFKQTWMDNMGRAGDMELKPFN-GEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNALKVVHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQELAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLTRLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDIREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMTEEKLAEAE-RVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILKDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNENSDSVAESGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKNKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKGGKAKGKKAQMS-EVLPSPHGKRVIPQVTMEMKAEAEK----KIRK--KIKSEN---VEGTPTENGLELG------SLKQRI-----EKKQKKEPGAMTKKQTTLAFKPIKKGKKRNPWSD----SESDMSS-----NESNVDVPPREKDPRRAAT------KAKFTMDLDSDEDFSGSDGKDEDEDFFPLDTTPPKTKIPQKNTKKALKPQKSAMSGDPESDEKDSVPASPGPPAADLPADTEQLKPSSKQTVAVKKTATKSQSSTSTAGTKKRAVPKGSKSDSALNAHGP-EKPVPAKAKNSRKRKQ 1463          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|3024753|sp|Q64399.1|TOP2B_CRILO (RecName: Full=DNA topoisomerase 2-beta; AltName: Full=DNA topoisomerase II, beta isozyme)

HSP 1 Score: 1607.04 bits (4160), Expect = 0.000e+0
Identity = 802/1352 (59.32%), Postives = 998/1352 (73.82%), Query Frame = 0

Query:   14 ANGDTQKANGEAKKANGEAKKANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKW----------KNAQKMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKG------AKGKKKLKI-EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGK---------KPWEDSDATSESD 1339
            A+G     N  AKK   E    N  +KK          LSVER+YQKK+QLEHILLRPDTYIGSV P+T+ MW+ DE+   +  +E+T+VPGLYKIFDEILVNAADNKQRDKNM  IK+ I+ E N I ++NNG+GIPV  HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETA +E +  FKQ W NNM K  EAK+K F  G DYT ITF PDL KF ME LD D VAL++RRAYD+A S KGVKV  NGK++ V  F+ YVD ++K   DETG  +K I+E A ERW+V + +S+ GFQQ+SFVNSIATTKGGRHVDYV   +   + EV+KKKNK GV++KP Q++NH+W+FINCLI NPTFDSQTKENMTL    FGSKC  + KFF      G+VES+L W KFKA  ++  K SS K +K+KGI KL+DANDAG K+S++CTLILTEGDSAK+LAV+GLGV+GRD++GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y   + LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLL+  FLEEFITPIVKA+K     SF+S+PE  EWK H +N   +KIKYYKGLGTST+KEAKEYF+DM+RH+ILF++   +DD AI +AFSKK ID RKEWLT +ME+ ++R+  GL E +LY   TK ++Y+DFINKEL+LFSN DNERSIPS+VDGFKPGQRKV+FTC KRNDKREVKVAQLAGSV EMSAYHHGE +LM TI+NLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT +S L RL+F + DD +L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW  K+PNY+ REIV N+  M+DG +P  M P +K+FKG I+ +   +Y V+GEI  +  N +E+TELPVR+WTQ YKEQV+EPMLNG++K PA+I DYKEYHTD TVKFVV+M+ +KL QAE   GLH+ FKLQTT++ +SMVLFD  GCL+KYE V +I++EFF LRL++Y  RK +L GML AES KL+NQARFILEK  G + +EN+ +K LI+ L ++G++SDPV+ W          ++ Q   +DS SDS  G     D++Y+L M++WSLT+E+ +EL++++D K +E+  L+  S  DLW+ DL  F+E+LD+VEA+E ED   IL+  S K           KKL++ E +PSP G R+VP I       A KA A  SRK  K  KK   +   VK EFD       +  +  E L  S       K   +   P  R + +P + GK          PW D ++ SESD
Sbjct:    9 ADGALTWVNNAAKKEELETSNKNDSSKK----------LSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDED-VGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFD-GEDYTCITFQPDLAKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEA-AGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVRAWKEAQEKAAEEEDTQNQHDDSSSDS--GTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQERED---ILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEIT------AMKADA--SRKLLK-KKKGDPDTPVVKVEFDEEFSGTPVEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPTSAGKPNAKKVKKRNPWSDDESKSESD 1333          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|13959709|sp|P11388.3|TOP2A_HUMAN (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme)

HSP 1 Score: 1602.8 bits (4149), Expect = 0.000e+0
Identity = 853/1565 (54.50%), Postives = 1069/1565 (68.31%), Query Frame = 0

Query:   36 NGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK---------------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEEL--KNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGSEVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHK--------VFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKK---PKAKK--PKASPKVKKPPAAATKKSKKTSQGSDYDDSD-------------------EDDMFKDSDLSVLSVEKKVRGGVRRAAAQQKFTYSSDESDF 1551
            N +  K K      KRLSVERIYQKK+QLEHILLRPDTYIGSV  VT++MW+ DE+   I  +E+T+VPGLYKIFDEILVNAADNKQRD  M  I++ I+ E N I ++NNG+GIPV  HK EKM+VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETASRE +K FKQ W +NM +A E +LK F+ G DYT ITF PDL KF M+SLD D VAL+ RRAYDIA S+K VKVFLNG ++ VK F+ YVD +LK   DETG+ +K I+E    RWEV + +S+ GFQQ+SFVNSIAT+KGGRHVDYV+  I + + +V+KKKNKGGV +K HQ++NH+W+F+N LI NPTFDSQTKENMTL    FGS C  + KF       G+VES+L W KFKA  ++  K S+ K N++KGI KL+DANDAG +NS +CTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y   D LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR  FLEEFITPIVK +K     +F+SLPE  EWKS T N   +K+KYYKGLGTSTSKEAKEYF+DMKRH+I FK+   +DD AI++AFSKK ID RKEWLT +ME+ ++RK LGL E YLY + T  ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMS+YHHGE SLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +S L RL+F   DD  L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW  KIPN++ REIV N+  ++DG EP  M P +K+FKG IE +   +YV++GE+A ++   IE++ELPVR+WTQ+YKEQV+EPMLNG+EK P +I DY+EYHTD TVKFVV+M+ +KL +AE   GLH+ FKLQT+++ +SMVLFD  GCL+KY+ VL+I+R+FF LRL +Y  RK +L GML AES KL+NQARFILEK DG +++ENK +K LI+ L ++G+DSDPVK WK AQ+                 E SDS ++ G      ++YLL M +W LT+E+KDEL R ++EK QEL  L+  S  DLW+ DL  F+E+L+ VEAKE +DE+V L  +  K    K ++  E LPSP G RV+P I  E+  + +       K+   E   +++  E+E +K        +K   R    K          KK+ + +  P K      K K + PW DS++   SD S    +FD    + +  ++  K+   +     E     +   +D      D+  PK K +   ++K        V DL+  D      L  S PA   P         D  +  +P+ KK  T+ K   K   S      KK   PK  K  P  +  V + P  A  K+++  + S  DDSD                    DD   D D +V    K V     RA    K+   SDE D 
Sbjct:   12 NMQVNKIKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDED-VGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFN-GEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAE-RVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPT----FNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMA-EVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRL-----EKKQKREPGTK---------TKKQTTLAFKPIK------KGKKRNPWSDSESDRSSDES----NFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFP---------DETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSV-----RAKKPIKYLEESDEDDL 1530          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|1174742|sp|P41516.1|TOP2A_RAT (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme)

HSP 1 Score: 1599.72 bits (4141), Expect = 0.000e+0
Identity = 829/1487 (55.75%), Postives = 1051/1487 (70.68%), Query Frame = 0

Query:   48 GAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK----------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSP-----KTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGSEVSDYEPEAKIAKNMAQNDIFNSSDDSD-----------APK-------VKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKK--KVLDSDDDAPIKKPKAKKPKASPKVKKPPAAATKKSKK 1499
            G KRLSVERIYQKK+QLEHILLRPDTYIGSV  VT++MW+ DE+   I  +E+T+VPGLYKIFDEILVNAADNKQRD  M  I++ + R  N I ++NNG+GIPV  HK EKM+VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETASRE +K FKQ W +NM +A + +LK FS G DYT ITF PDL KF M+SLD D VAL+ RRAYDIA S+K VKVFLNG R+ VK F+ YVD +LK   DETG+ +K ++E    RWEV + +S+ GFQQ+SFVNSIAT+KGGRHVDYV+  I S + +V+KKKNKGGV +K  Q++NH+W+F N +I NPTFDSQTKENMTL    FGS C  + KF       G+VES+L W KFKA  ++  K S+ K N++KGI KL+DANDAG++NS +CTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y   D LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR  FLEEFITPIVK +K     +F+SLPE  EWKS   N   +K+KYYKGLGTSTSKEAKEYF++MKRH+I FK+   +DD AI++AFSKK +D RKEWLT +ME+ ++RK LGL E YLY + T  ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMS+YHHGE SLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPL RL+F S DD  L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW  KIPN++ RE+V N+  ++DG EP  M P +K++KG IE + + +YV+NGE+A ++   IE+TELP+R+WTQ+YKEQV+EPMLNG+EK P +I DY+EYHTD TVKFV++M+ +KL +AE   GLH+ FKLQT+++ +SMVLFD  GCL+KY+ VL+I+R+FF LRL +Y  RK +L GML AES KL+NQARFILEK DG +V+ENK +K LI+ L ++G+DSDPVK WK AQ+           EE     +    E    ++YLL M +W LT+E+KDEL +++DEK QEL  L+  +  DLW+ DL  F+E+L+ VEAKE +DE+V L  +  K    K ++  E LPSPVG RV+P +  E++ +A K    K R+  K+  +N++   A          +      L ++L         K++  +  T  K+  + P     K + + PW D    SESD S    +FD    +      EP+  +       +AK   ++  +D F+  D+ D            PK        K+A  P      +D   +  D V  P++P A   ++D  +E   S  S       KRTI+K +       +   A  K+ K+     +   KKP  A  K S+K
Sbjct:   23 GKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDED-VGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTMMRN-NLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFS-GEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNRLPVKGFRSYVDMYLKDKVDETGNALKVVHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKADQVKNHMWIFGNAVIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSAECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSTNPNHKKWKVKYYKGLGTSTSKEAKEYFANMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTMYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPSKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNYKGTIEELASNQYVINGEVAILNSTTIEITELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMTEEKLAEAE-RVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPEEEENEENEESESESTSPAAESGPTFNYLLDMPLWYLTKEKKDELCKQRDEKEQELNTLKKKTPSDLWKEDLAAFVEELEVVEAKEKQDEQVGLPGKGVKAKGKKAQIS-EVLPSPVGKRVIPQVTMEMRAEAEK----KIRR--KIKSENVEGTPA----------EDGAEPGLRQRL--------EKRQKREPGTRAKKQTTLPFKPIKKAQKQNPWSD----SESDMSSNESNFDVPPRE-----KEPRIAAT------KAKFTADLDSDDDFSGLDEKDEDEDFFPLDDTPPKTKMPPKNTKKALKPQKSSTSVDLESDGKDSV--PASPGAS--AADVPAETEPSKPSSKQTVGVKRTITKGQSLTSTAGTKKRAVPKETKSDSALNAHVSKKPAPAKAKNSRK 1461          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1215154270|gb|ASK50299.1| (DNA topoisomerase 2 [Eyprepocnemis plorans])

HSP 1 Score: 1768.05 bits (4578), Expect = 0.000e+0
Identity = 836/1245 (67.15%), Postives = 1013/1245 (81.37%), Query Frame = 0

Query:   35 ANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK----MEEDSDS-DSEGGLEKDLD--YDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEA-------LPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKK 1265
            A G+A  P A     K ++VE+IYQKKSQLEHILLRPDTYIGSV PVTE MW+ D E + +V +EITYVPGLYKIFDEILVNAADNKQRD  MD IKIDI+ E N + V+NNG+GIPV  HKDEKM+VPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FSTKFTVETAS+E ++ FKQ W NNMSK  + K+KDF+ G DYT++TF+PDL KF ME+LD D V+L+SRRAYD+AAS++GVKV LNGKR+ VK FKDYV+ ++K  EDETG+P+K +YE   +RWEVA+ +SD GFQQ+SFVNSIATTKGGRHVD+VS MI   + E LKKKNK G+ IK  Q++NH+WLF+NCLIVNPTFDSQTKENMTL    FGSKC  + KF   + KSG+VES+L W+K KAD  +    + KK ++L GI KLEDAN AGT++S+DCTLILTEGDSAK+LAV+GLGV+GRDK+GV+PLRGKLLNVREA+HKQI++N EIN +++I+GL YKKKY +V+DLK+LRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLL+LPFLEEFITPIVKA+KG+   SF+SLPE  EWK+  DNWHTYKIKYYKGLGTSTSKEAKEYF DMKRH+I F +    DD  INMAFSKK I+QRKEWLT+WM++ KRRKE+GL E YLYEKDT +V+Y+DF+NKELVLFSN DNERSIP +VDGFKPGQRKV+FTCLKRNDKREVKVAQLAGSV E SAYHHGE SLM TIINLAQN+VGSNNIN+L PIGQFGTRL GGKD ASPRYIFT++SPL R IF+ +DDA+L + TDDNQ+IEP WY+P+IPMVLVNGA+GIGTGWMTKIPNYNPREI+ NL  MIDG  P+ M PW+K++KG IE   + +Y+V GEIA + E+K+E+TELPV  WTQ+YKE V+EPML G+EK+P  I  ++EY+TD TV+FVV+M   KL++AE  +GLH+FFKL+T++S +SM  FD   CL++Y++V+EI++EFF+LRLN+Y KRK YLEGML+AESL+LSNQARFILEKCDG LVVENKK+K +IEEL+ +G+D DPV++WK  Q     +EE +++ DS+    +D+D  YDYLLGM MW+LT+ERKDELL ++DEK QE  +L+G S  DLW+ DL   +E+LD+VEAKE EDE   +  +  KG K     K+ A       LPSP G RV P +  +   KA K  A K  KG++  KK
Sbjct:    2 AGGDA--PPAPKKNVKNMTVEKIYQKKSQLEHILLRPDTYIGSVEPVTELMWVYDPEQEMMVQREITYVPGLYKIFDEILVNAADNKQRDPKMDCIKIDIDAENNTVSVWNNGKGIPVVEHKDEKMYVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTKFTVETASKEYKRAFKQTWANNMSKTSDPKIKDFA-GEDYTKVTFSPDLTKFKMEALDKDIVSLMSRRAYDVAASTRGVKVMLNGKRLPVKTFKDYVELYIKNKEDETGNPLKIVYEQVNDRWEVALTISDKGFQQVSFVNSIATTKGGRHVDHVSDMIVKQLIETLKKKNKNGIQIKAFQVKNHMWLFVNCLIVNPTFDSQTKENMTLQAKNFGSKCPLSEKFINNVTKSGIVESILMWAKAKADSLLTKACNKKKQSRLTGIPKLEDANHAGTQHSLDCTLILTEGDSAKSLAVSGLGVIGRDKYGVFPLRGKLLNVREASHKQIMDNAEINNIIRIVGLQYKKKYESVNDLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLQLPFLEEFITPIVKASKGNQEFSFYSLPEFEEWKASKDNWHTYKIKYYKGLGTSTSKEAKEYFMDMKRHRIKFNYGGPNDDQCINMAFSKKCIEQRKEWLTSWMQDSKRRKEMGLPEQYLYEKDTHSVNYADFVNKELVLFSNCDNERSIPCMVDGFKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEHSAYHHGEQSLMSTIINLAQNYVGSNNINVLQPIGQFGTRLNGGKDSASPRYIFTKLSPLARFIFHKHDDALLKHNTDDNQRIEPVWYIPVIPMVLVNGANGIGTGWMTKIPNYNPREIIDNLRRMIDGEAPKQMNPWYKNYKGVIEPFGDSKYIVYGEIAVLGEDKVEITELPVGVWTQTYKENVLEPMLGGTEKVPQQIVQFQEYNTDTTVRFVVKMDPAKLQKAE-QEGLHKFFKLETSMSLNSMCAFDDMQCLKRYDSVIEILKEFFNLRLNYYGKRKLYLEGMLQAESLRLSNQARFILEKCDGVLVVENKKKKVIIEELKTRGYDPDPVRQWKLKQDKEALLEESNNADDSQDEATEDIDKLYDYLLGMTMWTLTKERKDELLSKRDEKLQEYEILKGKSPADLWKEDLDALLEELDKVEAKEREDEASGVVGKPVKGKKAAGSTKLAAISQKPVTLPSPGGRRVAPKLDPDRIKKAEKTTAVKENKGKRQVKK 1242          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1339043062|ref|XP_023709584.1| (DNA topoisomerase 2 isoform X2 [Cryptotermes secundus] >gi|1330910156|gb|PNF31624.1| DNA topoisomerase 2 [Cryptotermes secundus])

HSP 1 Score: 1756.88 bits (4549), Expect = 0.000e+0
Identity = 861/1351 (63.73%), Postives = 1063/1351 (78.68%), Query Frame = 0

Query:   36 NGEAKKPKAGGGGA------KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGS-EKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEED--SDSDSEG-----GLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILS-KESQKGAKGKKKLKIEAL------PSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS 1365
            NG+  +  +GG  A      K+L+VE+IYQKKSQLEHILLRPDTYIGSV PV E MW+ DEE   +V KEI+YVPGLYKIFDEILVNAADNKQRD  MD IKI+IN EKN I ++NNG+GIPV  HKDEKMFVPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FST F VETA++E ++ FKQVW +NMSK  E ++KD  +G D+T+ITF+PDL KF ME+LD D VAL+S+RA+D+AAS++GVKV+LNGKR+ VK+FKDYV+ +LKG ED+ G+P+K +YE    RWEVAV++S+ GFQQ+SFVNSIATTKGGRHVD++++ I   + E LKKKNKGG+ IKPHQ++ HLW+F+NCLIVNPTFDSQTKE MTL    FGSKC+ + KF   + KS +VE+VL W+K KA+++++ + SSKK NKLKG+ KLEDANDAG++NS+DCTLILTEGDSAKTL V+GLGVVGRDK+GV+PLRGKLLNVREATHKQI EN+EI  ++KI+GL YK KY ++DDLK+LRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLL+LPFLEEFITPIVKATKGS   SF+SLPE  EWK+ T+NWHTYKIKYYKGLGTSTSKEAKEYFSDM+RH+I FK+    DDH INMAFSKK  +QRKEWLT+WME+ KRR+ELGL E YLYEKDTK+V+YSDF+NKELVLFSN DNERSIPS+VDG KPGQRKV+FTCLKR DKRE+KVAQLAGSV E S+YHHGE SLM TI+NLAQNFVGSNNINLL PIGQFGTRL+GGKD ASPRYIFT +SPLT  IF+ +D  +L +  DD+QKIEP WY+PIIPMVLVNGADGIGTGWMTKIPNYNPREIV+NL  M+D  EPE M PW+K+F G +E + ++RY++ GEIA I E+K+E+TELP+ +WTQ+YKE V+EP+L+G+ EK P  I DYKEY+TD TVKFVV M+ +K R AE + GLH+FFKLQTT+S SSM +FD   C+RK+E+V  IM+EFF +RL +YEKRK Y EGML+AES +LSNQARFI+EKCDG+LV+ENKK K +IEEL+R G+D DPV  WK  Q   E    + D+EG     G +    Y+YLL MA+++LT+E+K++LLR++D+K  +L++L+  S   LW+ DL  F+E+LD+VEAKE EDE  + S K ++ G K     K E L      PSP G RV+P I  +LK K  K   +K  KG++  K+ L   E  KDEFD M  ++   ++L+++LG S  D   K         +++P+    ++  K         E+D+ VE  D + S  D+  S+  P++ S
Sbjct:    7 NGQVPEMMSGGDVAPKKPPNKKLTVEKIYQKKSQLEHILLRPDTYIGSVEPVVETMWLYDEEQDAMVQKEISYVPGLYKIFDEILVNAADNKQRDPKMDCIKIEINPEKNIISIWNNGQGIPVVEHKDEKMFVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTMFIVETAAKEYKRSFKQVWGSNMSKTSEPRIKD-HTGEDFTKITFSPDLSKFRMETLDKDIVALMSKRAFDVAASTRGVKVYLNGKRLPVKSFKDYVELYLKGKEDDQGNPLKCVYENVNPRWEVAVSLSEKGFQQVSFVNSIATTKGGRHVDHIAEHIVKQLIETLKKKNKGGLQIKPHQVKTHLWVFVNCLIVNPTFDSQTKETMTLQAKNFGSKCNLSDKFITNVTKSSIVEAVLTWAKSKAEKDLQKQCSSKKQNKLKGVPKLEDANDAGSRNSLDCTLILTEGDSAKTLVVSGLGVVGRDKYGVFPLRGKLLNVREATHKQIKENQEIGNVIKILGLQYKMKYESIDDLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLKLPFLEEFITPIVKATKGSTELSFYSLPEFEEWKASTNNWHTYKIKYYKGLGTSTSKEAKEYFSDMQRHRIKFKYGGPNDDHCINMAFSKKCAEQRKEWLTSWMEDTKRRRELGLPEQYLYEKDTKSVNYSDFVNKELVLFSNCDNERSIPSLVDGLKPGQRKVLFTCLKRADKREIKVAQLAGSVAEHSSYHHGEQSLMSTIVNLAQNFVGSNNINLLQPIGQFGTRLSGGKDSASPRYIFTLLSPLTSFIFHPHDMPLLKHNYDDSQKIEPVWYIPIIPMVLVNGADGIGTGWMTKIPNYNPREIVKNLKLMLDDQEPEPMVPWWKNFTGVVEPLVDKRYIIYGEIAVIGEDKLEITELPIGTWTQTYKESVLEPLLHGANEKTPPSITDYKEYNTDTTVKFVVTMNPEKRRAAEAE-GLHKFFKLQTTVSLSSMCVFDENCCMRKFESVELIMKEFFKVRLAYYEKRKKYHEGMLQAESRRLSNQARFIIEKCDGTLVIENKKMKVMIEELKRNGYDPDPVAVWKMKQNKAEALMENEDAEGSQLDDGSDVGGGYNYLLAMALYTLTEEKKEDLLRKRDDKLTQLKILQTKSPAALWKEDLDVFLEQLDKVEAKEKEDELGVSSGKTTKVGKKFGGSKKKEMLTAAETKPSPQGRRVIPKIDVKLKEKFEKETLSKENKGKRARKEKLLIEE--KDEFDMMDGNQ---KTLADRLGTSPEDIEKKMNAKRKEVKQRKPRKESSSRSPKKKGKGKNPWETDSEVE--DEELSSEDDGVSRPPPRTTS 1348          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1339043058|ref|XP_023709582.1| (DNA topoisomerase 2 isoform X1 [Cryptotermes secundus] >gi|1339043060|ref|XP_023709583.1| DNA topoisomerase 2 isoform X1 [Cryptotermes secundus])

HSP 1 Score: 1754.96 bits (4544), Expect = 0.000e+0
Identity = 861/1355 (63.54%), Postives = 1063/1355 (78.45%), Query Frame = 0

Query:   36 NGEAKKPKAGGGGA------KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGS-EKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEED------SDSDSEG-----GLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILS-KESQKGAKGKKKLKIEAL------PSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS 1365
            NG+  +  +GG  A      K+L+VE+IYQKKSQLEHILLRPDTYIGSV PV E MW+ DEE   +V KEI+YVPGLYKIFDEILVNAADNKQRD  MD IKI+IN EKN I ++NNG+GIPV  HKDEKMFVPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FST F VETA++E ++ FKQVW +NMSK  E ++KD  +G D+T+ITF+PDL KF ME+LD D VAL+S+RA+D+AAS++GVKV+LNGKR+ VK+FKDYV+ +LKG ED+ G+P+K +YE    RWEVAV++S+ GFQQ+SFVNSIATTKGGRHVD++++ I   + E LKKKNKGG+ IKPHQ++ HLW+F+NCLIVNPTFDSQTKE MTL    FGSKC+ + KF   + KS +VE+VL W+K KA+++++ + SSKK NKLKG+ KLEDANDAG++NS+DCTLILTEGDSAKTL V+GLGVVGRDK+GV+PLRGKLLNVREATHKQI EN+EI  ++KI+GL YK KY ++DDLK+LRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLL+LPFLEEFITPIVKATKGS   SF+SLPE  EWK+ T+NWHTYKIKYYKGLGTSTSKEAKEYFSDM+RH+I FK+    DDH INMAFSKK  +QRKEWLT+WME+ KRR+ELGL E YLYEKDTK+V+YSDF+NKELVLFSN DNERSIPS+VDG KPGQRKV+FTCLKR DKRE+KVAQLAGSV E S+YHHGE SLM TI+NLAQNFVGSNNINLL PIGQFGTRL+GGKD ASPRYIFT +SPLT  IF+ +D  +L +  DD+QKIEP WY+PIIPMVLVNGADGIGTGWMTKIPNYNPREIV+NL  M+D  EPE M PW+K+F G +E + ++RY++ GEIA I E+K+E+TELP+ +WTQ+YKE V+EP+L+G+ EK P  I DYKEY+TD TVKFVV M+ +K R AE + GLH+FFKLQTT+S SSM +FD   C+RK+E+V  IM+EFF +RL +YEKRK Y EGML+AES +LSNQARFI+EKCDG+LV+ENKK K +IEEL+R G+D DPV  WK  Q   E        + D+EG     G +    Y+YLL MA+++LT+E+K++LLR++D+K  +L++L+  S   LW+ DL  F+E+LD+VEAKE EDE  + S K ++ G K     K E L      PSP G RV+P I  +LK K  K   +K  KG++  K+ L   E  KDEFD M  ++   ++L+++LG S  D   K         +++P+    ++  K         E+D+ VE  D + S  D+  S+  P++ S
Sbjct:    7 NGQVPEMMSGGDVAPKKPPNKKLTVEKIYQKKSQLEHILLRPDTYIGSVEPVVETMWLYDEEQDAMVQKEISYVPGLYKIFDEILVNAADNKQRDPKMDCIKIEINPEKNIISIWNNGQGIPVVEHKDEKMFVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTMFIVETAAKEYKRSFKQVWGSNMSKTSEPRIKD-HTGEDFTKITFSPDLSKFRMETLDKDIVALMSKRAFDVAASTRGVKVYLNGKRLPVKSFKDYVELYLKGKEDDQGNPLKCVYENVNPRWEVAVSLSEKGFQQVSFVNSIATTKGGRHVDHIAEHIVKQLIETLKKKNKGGLQIKPHQVKTHLWVFVNCLIVNPTFDSQTKETMTLQAKNFGSKCNLSDKFITNVTKSSIVEAVLTWAKSKAEKDLQKQCSSKKQNKLKGVPKLEDANDAGSRNSLDCTLILTEGDSAKTLVVSGLGVVGRDKYGVFPLRGKLLNVREATHKQIKENQEIGNVIKILGLQYKMKYESIDDLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLKLPFLEEFITPIVKATKGSTELSFYSLPEFEEWKASTNNWHTYKIKYYKGLGTSTSKEAKEYFSDMQRHRIKFKYGGPNDDHCINMAFSKKCAEQRKEWLTSWMEDTKRRRELGLPEQYLYEKDTKSVNYSDFVNKELVLFSNCDNERSIPSLVDGLKPGQRKVLFTCLKRADKREIKVAQLAGSVAEHSSYHHGEQSLMSTIVNLAQNFVGSNNINLLQPIGQFGTRLSGGKDSASPRYIFTLLSPLTSFIFHPHDMPLLKHNYDDSQKIEPVWYIPIIPMVLVNGADGIGTGWMTKIPNYNPREIVKNLKLMLDDQEPEPMVPWWKNFTGVVEPLVDKRYIIYGEIAVIGEDKLEITELPIGTWTQTYKESVLEPLLHGANEKTPPSITDYKEYNTDTTVKFVVTMNPEKRRAAEAE-GLHKFFKLQTTVSLSSMCVFDENCCMRKFESVELIMKEFFKVRLAYYEKRKKYHEGMLQAESRRLSNQARFIIEKCDGTLVIENKKMKVMIEELKRNGYDPDPVAVWKMKQNKAEALENGMMENEDAEGSQLDDGSDVGGGYNYLLAMALYTLTEEKKEDLLRKRDDKLTQLKILQTKSPAALWKEDLDVFLEQLDKVEAKEKEDELGVSSGKTTKVGKKFGGSKKKEMLTAAETKPSPQGRRVIPKIDVKLKEKFEKETLSKENKGKRARKEKLLIEE--KDEFDMMDGNQ---KTLADRLGTSPEDIEKKMNAKRKEVKQRKPRKESSSRSPKKKGKGKNPWETDSEVE--DEELSSEDDGVSRPPPRTTS 1352          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|321457974|gb|EFX69050.1| (hypothetical protein DAPPUDRAFT_329527 [Daphnia pulex])

HSP 1 Score: 1746.48 bits (4522), Expect = 0.000e+0
Identity = 855/1279 (66.85%), Postives = 1035/1279 (80.92%), Query Frame = 0

Query:   35 ANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGS----LSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK--------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKG---AKGKKKLKI-EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVS 1297
             NG+A K     G  KRLSVERIYQKKSQLEHILLRPDTYIGSV PV + MW+  EE+  ++ +EITYVPGLYKIFDEILVNAADNK RD  MDTIK+DI+ ++N I ++NNG+GIPV  HK+EKM+VP+LIFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FST F +ETAS++ RK+FKQ W +NM+KA   K+ +++  +D+T+ITF+PDL KF M+SLD DTVALLSRRA+D+AAS++GVKVFLNGKR+ +KNFKDYVD  LKG ED+ G+ +K +YEA  ERWE+AVA SD GFQQ+SF NSIATTKGGRHVDYV++++   + E LKKKNK G+ +KPHQI+NHLW+F+NCLIVNPTFDSQTKENMTL    FGS C  + KFF  L KSGV+E V+ W+KFKA  E+    S KK  KLKG+ KLEDAN+AGTK+SIDCTLILTEGDSAK+LAV+GLGVVGRDK+GV+PL+GK+LNVREATHKQI+EN EIN ++KI+GL YKKKY +V+DLKSLRYG+LMIMTDQDQDGSHIKGLLINF+HHNWPSLLRLPFLEEFITPIVKATKG+     S SF+SLPE  EWK++T+NW TY+IKYYKGLGTSTS+EAK YFSDM+RH+ILF H+ DQDD+AI MAFSKK ++QRKEWLT+WMEECKRR+ELGL E+YLYEKDTK VSY DF+NKELVLFSN+DNERSIPS++DGFKPGQRKV+FTC+KRNDK+E+KVAQLAGSV E SAYHHGEASLMGTII LAQ+FVGSNNINLLLP GQFGTRL GGKD ASPRYIFT MS LTR+IF+ +DD +L +  DDNQ+IEPE+Y PIIPM+LVNG DGIGTGWMTKI NYNPRE+V N+  M+DG EP  M PWFK ++G IE I+  RY ++GE+A +S  ++E+TELP+R WTQ+YKE VME +L+GSEKIP +I DYKEYHTD TV+F+V MS +K++QAE + GLH+ FKLQTT S +SMVL+D+ GCL+ Y+ V +I++EFF +RL +Y KRK+YL G+L+AES +LSNQARFI EKCD  L+VENKK K L+EEL+R+ +D DPV+ WK  QK         EE++++++E G  K  DYDYLL M M SLT E+K+ELL+++D K +E  +L   S  DLWR DL  FM  L EVE  + E E +  +K+  K    AKG+KK  I E  PSP G R+VP+I  EL+ K  K   AK +K EK  KK   EVE  +D FD   DD   N+SL EKLGVS
Sbjct:   25 TNGDAGKK---NGANKRLSVERIYQKKSQLEHILLRPDTYIGSVEPVKDVMWVY-EEDAGMIQREITYVPGLYKIFDEILVNAADNKVRDPKMDTIKVDIDPKENVITIYNNGKGIPVVEHKEEKMYVPSLIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTSFELETASKDERKKFKQTWTDNMTKAGTPKIVNYNE-ADFTQITFSPDLAKFKMDSLDKDTVALLSRRAFDVAASTRGVKVFLNGKRLPIKNFKDYVDLCLKGKEDDDGAQLKCVYEAVSERWEIAVAPSDKGFQQVSFANSIATTKGGRHVDYVTEIVSKYLQEFLKKKNKSGINVKPHQIKNHLWIFVNCLIVNPTFDSQTKENMTLQAKSFGSTCKPSEKFFNALNKSGVIEGVMTWAKFKAQTELNKACSGKKQTKLKGVPKLEDANNAGTKHSIDCTLILTEGDSAKSLAVSGLGVVGRDKYGVFPLKGKVLNVREATHKQILENAEINNLIKILGLQYKKKYESVEDLKSLRYGRLMIMTDQDQDGSHIKGLLINFLHHNWPSLLRLPFLEEFITPIVKATKGTGASAKSLSFYSLPEFEEWKTNTENWPTYRIKYYKGLGTSTSQEAKGYFSDMQRHRILFHHDGDQDDYAILMAFSKKHVEQRKEWLTSWMEECKRRRELGLPELYLYEKDTKRVSYRDFVNKELVLFSNMDNERSIPSLMDGFKPGQRKVLFTCIKRNDKKEIKVAQLAGSVAEHSAYHHGEASLMGTIIGLAQDFVGSNNINLLLPNGQFGTRLQGGKDAASPRYIFTLMSKLTRIIFHPHDDPLLKHQMDDNQRIEPEYYAPIIPMILVNGTDGIGTGWMTKILNYNPREVVANVKRMLDGEEPIPMDPWFKGYRGTIERIDRHRYAISGELAVLSGTEMEITELPLRVWTQNYKESVMETLLHGSEKIPPLITDYKEYHTDTTVRFIVTMSEEKMQQAEAE-GLHKAFKLQTTHSLTSMVLYDSNGCLKMYDTVEDILKEFFTVRLGYYGKRKDYLSGVLQAESQRLSNQARFICEKCDNVLIVENKKAKVLVEELRRRNYDPDPVRIWKKNQKNNVNAENEEEEEANAENEEGAVKIGDYDYLLDMPMRSLTYEKKEELLKKRDVKMKEYDILLKKSPADLWREDLDHFMVVLKEVE--DAEQEELNENKKPSKAAPLAKGRKKALIAEVAPSPKGRRIVPVIDNELRKKIEKLTIAKEKKKEKALKKESPEVE--RDSFDIALDDS--NKSLGEKLGVS 1291          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1325310473|ref|XP_023336845.1| (LOW QUALITY PROTEIN: DNA topoisomerase 2-alpha-like [Eurytemora affinis])

HSP 1 Score: 1739.16 bits (4503), Expect = 0.000e+0
Identity = 880/1302 (67.59%), Postives = 1049/1302 (80.57%), Query Frame = 0

Query:   39 AKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRN-DKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ--------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRR--KDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPW 1329
            A KPK    G KRLSVERIYQKKSQLEHILLRPDTYIGSV PVTE MW+ ++EN  +VN+E+TYVPG YKI+DEILVNAADNKQRD  MDTIKIDI  E+  I + NNG+GIPV MHK+E MFVPT+IFGHLLTSSNFNDEEEKVTGGRNG+GAKLCNVFSTKFTVETA ++  K FKQ W +NMSKA E K+KDF+ G +YT+IT++PDL KFNME +D DT AL SRRA+DIAAS+KGVKVFLNGKR+ VK+FKDYVD ++KG EDE+G+ +K I+EA G RWEVAV VSD GFQQMSFVNSIATTKGGRHVD+V+  +   I + +KK NK G+ IK  Q++NHLWLF+NCLIVNPTFDSQTKE MTL +  FGSKC  + KF   LQKSG++ESV+AWSKFKAD ++ +K   KKT+KL+GI KLEDAN+AGTKNS  CTLILTEGDSAKTLAVAGLGV+GRD +GVYPL+GKLLNVREATHKQI+ENKEIN++VKIIGL YKKKY T++D+KSLRYGK++I+   + D   IKGL+INFIHHNWP LL+LPFLEEFITPIVK +      SF+        K   D W   +  ++ GLGTSTSKEAKEYFSDM RH+I FK+   QDDH+I +AFSKK I+ RKEWLTTWMEE KRRKELGL E+YLYEKDTK V+Y+DF+NKELVLFSN+DNERSIPS+VDGFKPGQRKV+FTCLKRN  K+E+KVAQLAGSV E+SAYHHGEASLMGTIINLAQN+VGSNNINLL PIGQFGTRL GGKD ASPRYIFTQ+SPL R IFN  DDA+LNY  DDNQKIEPEWY+PI+PMVLVNGADGIGTGWMTKIPNYNPREIV+NL  MIDG EP+ M PWFK FKG IE I++QRY V+GEI+++S+ K+E+TELP+R+WT SYKE ++E +LNG+EK+PA+I D+K+Y+TD TVKF++ M  DKLR AE +KGLH FFKLQT+IS +SMVLFD  GCLR++++V EI+REFF LRL FY KRK YLEGML AE+ KLSNQARFILEKCD +L +ENKK+K +IEELQR+G+DSDP+K WK +Q          +E++     GG     DYDYLLGM +W LTQE+KDELL++  +  +   + +L  TSKE+LW+ DL  F+ KLDE E  E+ D+    +K  +KG   K  LK+EALPSP+GIRV P IA+ELK K AKA AAK+RK +K DKK++K+ E  KDEFD M +DK++N+SLSE++G    +K  K+      T     +SSPK KGK PW
Sbjct:   16 APKPK----GDKRLSVERIYQKKSQLEHILLRPDTYIGSVQPVTEPMWVYEDEN--MVNREVTYVPGFYKIYDEILVNAADNKQRDPKMDTIKIDIKPEEGVISIMNNGKGIPVVMHKEENMFVPTMIFGHLLTSSNFNDEEEKVTGGRNGFGAKLCNVFSTKFTVETADKKAGKGFKQTWASNMSKAGEPKIKDFN-GEEYTKITYSPDLKKFNMEKIDDDTFALFSRRAFDIAASTKGVKVFLNGKRLPVKSFKDYVDQYIKGKEDESGNQIKCIHEACGPRWEVAVCVSDKGFQQMSFVNSIATTKGGRHVDHVADAVIKAIIDKIKKSNKTGINIKNFQVKNHLWLFVNCLIVNPTFDSQTKETMTLGVKDFGSKCVLSDKFQTSLQKSGIIESVVAWSKFKADMKLSSKAGGKKTSKLRGIAKLEDANEAGTKNSGGCTLILTEGDSAKTLAVAGLGVIGRDYYGVYPLKGKLLNVREATHKQILENKEINDLVKIIGLAYKKKYETMEDIKSLRYGKVLILLKLEFDTPQIKGLVINFIHHNWPGLLKLPFLEEFITPIVKVSHYRSLMSFYH-------KVGYDFW--VQFIHFIGLGTSTSKEAKEYFSDMVRHRIKFKYNGSQDDHSITLAFSKKEIEGRKEWLTTWMEEGKRRKELGLPEVYLYEKDTKAVNYTDFVNKELVLFSNMDNERSIPSLVDGFKPGQRKVVFTCLKRNLTKKEIKVAQLAGSVAEISAYHHGEASLMGTIINLAQNYVGSNNINLLQPIGQFGTRLQGGKDSASPRYIFTQLSPLARHIFNEADDALLNYQLDDNQKIEPEWYIPILPMVLVNGADGIGTGWMTKIPNYNPREIVKNLKLMIDGKEPKPMIPWFKGFKGTIEEIDSQRYAVSGEISTLSDTKVEITELPIRTWTNSYKE-LLEGLLNGTEKLPAVISDFKDYNTDRTVKFIITMDKDKLRNAEREKGLHAFFKLQTSISNTSMVLFDHLGCLRRFDSVEEILREFFTLRLEFYGKRKRYLEGMLGAEAAKLSNQARFILEKCDNTLTIENKKKKVMIEELQRRGYDSDPIKAWKKSQDNMDEEAAGEDEEAAESPAGG----PDYDYLLGMKLWCLTQEKKDELLKKLWRCXRINXIYILXATSKEELWKVDLDAFLAKLDETEDAELADD-AGAAKLGKKGPGKKGALKLEALPSPMGIRVEPRIADELKVKVAKAVAAKARKADKGDKKSVKK-EDDKDEFDLMTEDKELNKSLSERIGAVKKEKPLKQ------TKLNFEKSSPKKKGKNPW 1288          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1316162989|ref|XP_023226762.1| (DNA topoisomerase 2-alpha-like isoform X1 [Centruroides sculpturatus] >gi|1316162991|ref|XP_023226763.1| DNA topoisomerase 2-alpha-like isoform X2 [Centruroides sculpturatus])

HSP 1 Score: 1736.08 bits (4495), Expect = 0.000e+0
Identity = 843/1317 (64.01%), Postives = 1043/1317 (79.20%), Query Frame = 0

Query:   50 KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGGLEKDL---DYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKES---QKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAK----AAAAKSR-KGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKK------PWEDSDATSESDNSVEFDDFDD 1349
            KRLSVERIYQKK+QLEHILLRPDTYIGSV PVT+ MW+ D     ++ +++++VPGLYKIFDEILVNAADNKQRDK+MD IK+DI+ E N+I ++NNG+GIPV  HK EK+FVP+LIFGHLLTSSN+NDEE+KVTGGRNGYGAKLCN+FSTKF VET+S+E +K FKQ W +NMSK  EA++   + G D+T +TF+PDL KF ME LD DTVAL+SRR YD+A SS+GVKV+LNGKR+ ++ FK+YV+ ++K   DE G+P+K +YE+  +RWEVAV +S+ GFQQ+SFVNSIATTKGGRHVDYV++ I S +A+V+KKKNKGGV IKP QI+NH+W+F+NCLI NPTFDSQTKENMTL    FGSKC  + KFF Q+ K G++E +++W +FKA  E+  K S+ K +KL+GI KLEDANDAGTK SIDCTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREATHKQI+EN+EIN ++KI+GL YKKKY ++DDLK+LRYG+LMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVK +KG    SF+SLPE  EWK  T NW+T+KIKYYKGLGTSTSKEAKEYF+DM RH+I FK+E  QDD+AI +AFSKK +DQRKEWLT  MEE K R+ELGL E+YLY K+T  ++++DF+NKEL+LFSN+DNERSIPS+VDG KPGQRKVM+TC KRNDKRE+KVAQLAGSV E SAYHHGE++LMGTIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT +SPL +LIF+  D+ +L +L DDNQ+IEPE+Y+P+IPMVLVNG +GIGTGW TKIPNYNPREIV N+  M+ G EP  M PWFK+FKG IE ++NQRYV+NGE++ +S NK+E+TELPVR+WTQ+YKEQV+E ML+GS+K+P++I DYKEYHTD TV+FVV M+ +KL ++E ++GLH+ FKLQTT+STSSMVLFD KGCLR+Y++  EI++EFF +RL+ YE+RK YLEGML AES KL +QARFILEK +G +++EN+K+  LI  LQ +G++SDPVK WK +Q    D++  +E   E+D    DYDYLLGM +WSLT+ERKDELL+++D+K  EL  LR  +  DLW  DL   M +LD VE KE EDE +  +K++    K  KG+K    E  PSP+G RV+P I  +LK +A K        K R KG+K D + +KE          +C++ +      E LG S      KK+   SST  K  + S   KG K      PW      +ES+NS    D DD
Sbjct:   30 KRLSVERIYQKKTQLEHILLRPDTYIGSVEPVTQSMWVYDGPETGMIFRDVSFVPGLYKIFDEILVNAADNKQRDKSMDCIKVDIDPENNKISIWNNGKGIPVVEHKVEKIFVPSLIFGHLLTSSNYNDEEKKVTGGRNGYGAKLCNIFSTKFVVETSSKEYKKSFKQCWTDNMSKTTEARIIP-NKGEDFTCVTFSPDLSKFGMEVLDKDTVALMSRRVYDVAGSSRGVKVYLNGKRLPIRGFKEYVEQYIKDKNDEQGNPLKVVYESVNDRWEVAVTMSERGFQQVSFVNSIATTKGGRHVDYVTEQIVSKLADVVKKKNKGGVQIKPFQIKNHMWVFVNCLIENPTFDSQTKENMTLQAKNFGSKCPLSDKFFNQVIKCGIIECIMSWVRFKAQTELNKKCSATKHSKLRGIPKLEDANDAGTKGSIDCTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKMLNVREATHKQILENQEINNIIKIMGLQYKKKYESIDDLKTLRYGRLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKISKGKEEMSFYSLPEYEEWKQQTPNWNTWKIKYYKGLGTSTSKEAKEYFADMNRHRIKFKYEGTQDDNAIQLAFSKKLVDQRKEWLTNGMEERKHRRELGLPELYLYGKNTTNITFNDFVNKELILFSNMDNERSIPSLVDGLKPGQRKVMYTCFKRNDKREIKVAQLAGSVAEHSAYHHGESALMGTIINLAQNFVGSNNINLLQPIGQFGTRLQGGKDAASPRYIFTMLSPLAKLIFSQLDEPLLVHLFDDNQRIEPEYYIPVIPMVLVNGCEGIGTGWSTKIPNYNPREIVSNIRRMLKGEEPVKMNPWFKNFKGVIEQLDNQRYVINGEVSILSSNKLEITELPVRTWTQTYKEQVLETMLHGSDKVPSLITDYKEYHTDSTVRFVVTMNEEKLSKSE-EQGLHKVFKLQTTLSTSSMVLFDHKGCLRRYDSPEEILKEFFEVRLSCYERRKAYLEGMLTAESKKLDSQARFILEKIEGKIIIENRKKSELIRTLQERGYESDPVKSWKESQNKNTDNEETNEDDEERDSSGPDYDYLLGMNLWSLTRERKDELLKKRDDKRAELEALREKTPADLWEDDLTNLMVELDRVEEKEREDEIIGGTKKAIKLGKSGKGRKVALEETKPSPMGQRVIPHIDPDLKKRAEKTPLGTGEGKRRGKGKKTDSELIKE----------LCENGE------EGLGNSDKSNKMKKKLKCSSTLLKAVKKSGSPKGNKKGPKRNPWSSESDGNESNNS----DVDD 1324          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1067070039|ref|XP_018018369.1| (PREDICTED: DNA topoisomerase 2-alpha-like isoform X3 [Hyalella azteca])

HSP 1 Score: 1734.15 bits (4490), Expect = 0.000e+0
Identity = 881/1489 (59.17%), Postives = 1109/1489 (74.48%), Query Frame = 0

Query:   43 KAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ-------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVI---LSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPK-----KRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS--------EVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKKPPAAATKKSKKTSQGSDYDD 1508
            +A     K+ +VE+ YQKK+QLEHI+LRPDTYIGSV  VTE+MW+ D  +++ V +EITYVPGLYKIFDEILVNAADNKQRD  MDTIKI+I+ E N I V+NNG GIPV MHKDEKMFVPT+IFGHLLTSSN+ND+E+KVTGGRNG+GAKLCN+FSTKFTVETA++E +K FKQ W  NMSKA + K+KDF+ G +YT+ITF+PDL KF M+ LD DTV LLSRRAYD+AASS GVKV+LNGKR+ VK+FKDYV+ ++K   D+ G+P+K +YE  G RWEVAV +S+ GFQ MSFVNSIATTKGGRHVDY++ MI +++ E L KKNK GV IK  QI+NHLW+F+N LI NPTFDSQTKENMTL    FGSKC+ + KF   + KSG+VE+V+AW+KFK   +++ K + KKT+KLKGI KLEDANDAGT++S +CTLILTEGDSAKTLAVAGLGVVGRDK+GV+PLRGK+LN REAT KQ++EN EIN +++I+GL YKKKY TVDDLK+LRYGKLMIMTDQDQDGSHIKGL+INFIHHNWPSLLRL FLEEFITPIVKATKG    SF+SLPE  EWKS+ DNWHTYKIKYYKGLGTSTSKEAKEYFSDM RH+I FK+E DQDD +I MAFSKKA DQRKEWLT WM ECKRR+ELGL EIYLYEK+T +V+YSDF+NKELVLFSN DNERSIPS+VDGFKPGQRKVMFTC+KRNDKREVKVAQLAGS+ E +AYHHGE SLM TIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT++SPL R + + +DD +L YL +DNQKIEPEWYMPIIP+VLVNGA+GIGTGW TKIPNYNPREIV N+  MI G EP+DM PWFK+F+G +E ++  RY+ +GE+A + +N+ E++ELP+R+WTQSYKE V+E +L G++KIP II DY+EY+TD TV+FV+ +  +++R  E + G H+ FKLQ+++S ++MVLFDA G L++Y +V+++++EF+ LRL FYEKRK Y+ GML+AE+ KLSNQARFI EKC+G LVVENKKRK +I EL  KG++SDPVK WK +Q           +     E   +   D+DYLLGM MWSLT+ERK++LL+++ EK  EL  L+  S+ DLW  DL  F+E+LD+VEAKE E+ERV    L+ + +K    KKK  +E +PSP+G R+ P I  E++ KA K+AAA + K  +  K++  + +    +        +  +SL+ +LG S +    KKR +   T K     K+ + +   KGK PW  SD+ ++  NS       DS       ++ P+S +          + Y        +    +  +S+D  D    +R   P    F LD ++++      P AP+  +P++ +         S++P  KK    S     + D+D +    + K KKP A        AA+TK++  T+ GS+  D
Sbjct:   29 QAAEPAGKKRTVEKTYQKKTQLEHIILRPDTYIGSVEKVTEQMWVFDTADEKFVQREITYVPGLYKIFDEILVNAADNKQRDPKMDTIKIEIDAENNVISVYNNGHGIPVVMHKDEKMFVPTMIFGHLLTSSNYNDDEKKVTGGRNGFGAKLCNIFSTKFTVETANKEYKKNFKQTWAVNMSKASDPKIKDFT-GEEYTKITFSPDLAKFGMDCLDRDTVDLLSRRAYDVAASSTGVKVYLNGKRVPVKSFKDYVELYIKDRTDDAGAPLKVVYEKPGPRWEVAVTISEKGFQHMSFVNSIATTKGGRHVDYITNMIVNSLTETLSKKNKSGVQIKNFQIKNHLWVFVNALIENPTFDSQTKENMTLQQKSFGSKCTLSDKFVAAVVKSGIVETVMAWAKFKQQAQLEKKCAGKKTSKLKGIPKLEDANDAGTRSSSECTLILTEGDSAKTLAVAGLGVVGRDKYGVFPLRGKVLNTREATVKQMMENAEINHIIQIVGLQYKKKYETVDDLKTLRYGKLMIMTDQDQDGSHIKGLIINFIHHNWPSLLRLNFLEEFITPIVKATKGKEEISFYSLPEYEEWKSNKDNWHTYKIKYYKGLGTSTSKEAKEYFSDMVRHRIKFKYEGDQDDRSIQMAFSKKAADQRKEWLTAWMLECKRRRELGLPEIYLYEKNTHSVNYSDFVNKELVLFSNADNERSIPSLVDGFKPGQRKVMFTCIKRNDKREVKVAQLAGSIAEHTAYHHGEVSLMSTIINLAQNFVGSNNINLLEPIGQFGTRLMGGKDAASPRYIFTKLSPLARALIHPHDDPLLKYLQEDNQKIEPEWYMPIIPLVLVNGAEGIGTGWSTKIPNYNPREIVDNIRKMIRGEEPKDMLPWFKNFRGTVEQLDATRYLCSGEVARLEDNQYEISELPIRTWTQSYKENVLE-ILQGNDKIPPIISDYREYNTDTTVRFVLNVLDNQVRHVEAE-GPHKIFKLQSSMSINTMVLFDANGVLKRYNSVMDVLQEFYELRLKFYEKRKLYMVGMLKAEANKLSNQARFIKEKCEGKLVVENKKRKDMINELITKGYESDPVKAWKKSQSREAAVEDEAAEESDAEEEQTDDAADFDYLLGMPMWSLTKERKEDLLKKEQEKKHELETLQAKSESDLWNHDLDVFIEELDKVEAKEREEERVAKENLASKDKKAGGKKKKALLEVMPSPMGERIQPRIDAEMRKKAEKSAAAGAAKAGRPKKRDNPDDDHDDLD---DLVSANTGKSLASRLGTSPD--LVKKRKAGRPTKKNGDGLKQTKINFPKKGKNPWS-SDSDADGGNS-------DSDLSTTNIETVPRSRAVEGKRAAAAKAKYNFSDDDEDDDDDIESLHSNDGVD----ERHVGPEKLSF-LDQTEDN----AAPDAPSPPKPAAKK---------SNAPKPKKANAHSDEDIVISDTDSEFGDFQQKKKKPSA--------AASTKQAASTADGSNASD 1475          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1067070035|ref|XP_018018366.1| (PREDICTED: DNA topoisomerase 2-alpha-like isoform X1 [Hyalella azteca])

HSP 1 Score: 1731.84 bits (4484), Expect = 0.000e+0
Identity = 880/1496 (58.82%), Postives = 1107/1496 (74.00%), Query Frame = 0

Query:   43 KAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ-------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLK----------IEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPK-----KRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS--------EVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKKPPAAATKKSKKTSQGSDYDD 1508
            +A     K+ +VE+ YQKK+QLEHI+LRPDTYIGSV  VTE+MW+ D  +++ V +EITYVPGLYKIFDEILVNAADNKQRD  MDTIKI+I+ E N I V+NNG GIPV MHKDEKMFVPT+IFGHLLTSSN+ND+E+KVTGGRNG+GAKLCN+FSTKFTVETA++E +K FKQ W  NMSKA + K+KDF+ G +YT+ITF+PDL KF M+ LD DTV LLSRRAYD+AASS GVKV+LNGKR+ VK+FKDYV+ ++K   D+ G+P+K +YE  G RWEVAV +S+ GFQ MSFVNSIATTKGGRHVDY++ MI +++ E L KKNK GV IK  QI+NHLW+F+N LI NPTFDSQTKENMTL    FGSKC+ + KF   + KSG+VE+V+AW+KFK   +++ K + KKT+KLKGI KLEDANDAGT++S +CTLILTEGDSAKTLAVAGLGVVGRDK+GV+PLRGK+LN REAT KQ++EN EIN +++I+GL YKKKY TVDDLK+LRYGKLMIMTDQDQDGSHIKGL+INFIHHNWPSLLRL FLEEFITPIVKATKG    SF+SLPE  EWKS+ DNWHTYKIKYYKGLGTSTSKEAKEYFSDM RH+I FK+E DQDD +I MAFSKKA DQRKEWLT WM ECKRR+ELGL EIYLYEK+T +V+YSDF+NKELVLFSN DNERSIPS+VDGFKPGQRKVMFTC+KRNDKREVKVAQLAGS+ E +AYHHGE SLM TIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT++SPL R + + +DD +L YL +DNQKIEPEWYMPIIP+VLVNGA+GIGTGW TKIPNYNPREIV N+  MI G EP+DM PWFK+F+G +E ++  RY+ +GE+A + +N+ E++ELP+R+WTQSYKE V+E +L G++KIP II DY+EY+TD TV+FV+ +  +++R  E + G H+ FKLQ+++S ++MVLFDA G L++Y +V+++++EF+ LRL FYEKRK Y+ GML+AE+ KLSNQARFI EKC+G LVVENKKRK +I EL  KG++SDPVK WK +Q           +     E   +   D+DYLLGM MWSLT+ERK++LL+++ EK  EL  L+  S+ DLW  DL  F+E+LD+VEAKE E+ERV  +KE+      K   K          +E +PSP+G R+ P I  E++ KA K+AAA + K  +  K++  + +    +        +  +SL+ +LG S +    KKR +   T K     K+ + +   KGK PW  SD+ ++  NS       DS       ++ P+S +          + Y        +    +  +S+D  D    +R   P    F LD ++++      P AP+  +P++ +         S++P  KK    S     + D+D +    + K KKP A        AA+TK++  T+ GS+  D
Sbjct:   29 QAAEPAGKKRTVEKTYQKKTQLEHIILRPDTYIGSVEKVTEQMWVFDTADEKFVQREITYVPGLYKIFDEILVNAADNKQRDPKMDTIKIEIDAENNVISVYNNGHGIPVVMHKDEKMFVPTMIFGHLLTSSNYNDDEKKVTGGRNGFGAKLCNIFSTKFTVETANKEYKKNFKQTWAVNMSKASDPKIKDFT-GEEYTKITFSPDLAKFGMDCLDRDTVDLLSRRAYDVAASSTGVKVYLNGKRVPVKSFKDYVELYIKDRTDDAGAPLKVVYEKPGPRWEVAVTISEKGFQHMSFVNSIATTKGGRHVDYITNMIVNSLTETLSKKNKSGVQIKNFQIKNHLWVFVNALIENPTFDSQTKENMTLQQKSFGSKCTLSDKFVAAVVKSGIVETVMAWAKFKQQAQLEKKCAGKKTSKLKGIPKLEDANDAGTRSSSECTLILTEGDSAKTLAVAGLGVVGRDKYGVFPLRGKVLNTREATVKQMMENAEINHIIQIVGLQYKKKYETVDDLKTLRYGKLMIMTDQDQDGSHIKGLIINFIHHNWPSLLRLNFLEEFITPIVKATKGKEEISFYSLPEYEEWKSNKDNWHTYKIKYYKGLGTSTSKEAKEYFSDMVRHRIKFKYEGDQDDRSIQMAFSKKAADQRKEWLTAWMLECKRRRELGLPEIYLYEKNTHSVNYSDFVNKELVLFSNADNERSIPSLVDGFKPGQRKVMFTCIKRNDKREVKVAQLAGSIAEHTAYHHGEVSLMSTIINLAQNFVGSNNINLLEPIGQFGTRLMGGKDAASPRYIFTKLSPLARALIHPHDDPLLKYLQEDNQKIEPEWYMPIIPLVLVNGAEGIGTGWSTKIPNYNPREIVDNIRKMIRGEEPKDMLPWFKNFRGTVEQLDATRYLCSGEVARLEDNQYEISELPIRTWTQSYKENVLE-ILQGNDKIPPIISDYREYNTDTTVRFVLNVLDNQVRHVEAE-GPHKIFKLQSSMSINTMVLFDANGVLKRYNSVMDVLQEFYELRLKFYEKRKLYMVGMLKAEANKLSNQARFIKEKCEGKLVVENKKRKDMINELITKGYESDPVKAWKKSQSREAAVEDEAAEESDAEEEQTDDAADFDYLLGMPMWSLTKERKEDLLKKEQEKKHELETLQAKSESDLWNHDLDVFIEELDKVEAKEREEERV--AKENLASKDKKAGGKKKKKYVDDALLEVMPSPMGERIQPRIDAEMRKKAEKSAAAGAAKAGRPKKRDNPDDDHDDLD---DLVSANTGKSLASRLGTSPD--LVKKRKAGRPTKKNGDGLKQTKINFPKKGKNPWS-SDSDADGGNS-------DSDLSTTNIETVPRSRAVEGKRAAAAKAKYNFSDDDEDDDDDIESLHSNDGVD----ERHVGPEKLSF-LDQTEDN----AAPDAPSPPKPAAKK---------SNAPKPKKANAHSDEDIVISDTDSEFGDFQQKKKKPSA--------AASTKQAASTADGSNASD 1480          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1067070037|ref|XP_018018367.1| (PREDICTED: DNA topoisomerase 2-alpha-like isoform X2 [Hyalella azteca])

HSP 1 Score: 1730.3 bits (4480), Expect = 0.000e+0
Identity = 882/1497 (58.92%), Postives = 1103/1497 (73.68%), Query Frame = 0

Query:   43 KAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ-------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLK----------IEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPK-----KRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS--------EVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKKPPAAA-TKKSKKTSQGSDYDD 1508
            +A     K+ +VE+ YQKK+QLEHI+LRPDTYIGSV  VTE+MW+ D  +++ V +EITYVPGLYKIFDEILVNAADNKQRD  MDTIKI+I+ E N I V+NNG GIPV MHKDEKMFVPT+IFGHLLTSSN+ND+E+KVTGGRNG+GAKLCN+FSTKFTVETA++E +K FKQ W  NMSKA + K+KDF+ G +YT+ITF+PDL KF M+ LD DTV LLSRRAYD+AASS GVKV+LNGKR+ VK+FKDYV+ ++K   D+ G+P+K +YE  G RWEVAV +S+ GFQ MSFVNSIATTKGGRHVDY++ MI +++ E L KKNK GV IK  QI+NHLW+F+N LI NPTFDSQTKENMTL    FGSKC+ + KF   + KSG+VE+V+AW+KFK   +++ K + KKT+KLKGI KLEDANDAGT++S +CTLILTEGDSAKTLAVAGLGVVGRDK+GV+PLRGK+LN REAT KQ++EN EIN +++I+GL YKKKY TVDDLK+LRYGKLMIMTDQDQDGSHIKGL+INFIHHNWPSLLRL FLEEFITPIVKATKG    SF+SLPE  EWKS+ DNWHTYKIKYYKGLGTSTSKEAKEYFSDM RH+I FK+E DQDD +I MAFSKKA DQRKEWLT WM ECKRR+ELGL EIYLYEK+T +V+YSDF+NKELVLFSN DNERSIPS+VDGFKPGQRKVMFTC+KRNDKREVKVAQLAGS+ E +AYHHGE SLM TIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT++SPL R + + +DD +L YL +DNQKIEPEWYMPIIP+VLVNGA+GIGTGW TKIPNYNPREIV N+  MI G EP+DM PWFK+F+G +E ++  RY+ +GE+A + +N+ E++ELP+R+WTQSYKE V+E +L G++KIP II DY+EY+TD TV+FV+ +  +++R  E + G H+ FKLQ+++S ++MVLFDA G L++Y +V+++++EF+ LRL FYEKRK Y+ GML+AE+ KLSNQARFI EKC+G LVVENKKRK +I EL  KG++SDPVK WK +Q           +     E   +   D+DYLLGM MWSLT+ERK++LL+++ EK  EL  L+  S+ DLW  DL  F+E+LD+VEAKE E+ERV  +KE+      K   K          +E +PSP+G R+ P I  E++ KA K+AAA + K  +  K++  + +    +        +  +SL+ +LG S +    KKR +   T K     K+ + +   KGK PW  SD+ ++  NS       DS       ++ P+S +          + Y        +    +  +S+D  D    +R   P    F LD ++++      P AP+  +P+                   KK    K KK    SD+D  I    ++      K KKP AAA TK++  T+ GS+  D
Sbjct:   29 QAAEPAGKKRTVEKTYQKKTQLEHIILRPDTYIGSVEKVTEQMWVFDTADEKFVQREITYVPGLYKIFDEILVNAADNKQRDPKMDTIKIEIDAENNVISVYNNGHGIPVVMHKDEKMFVPTMIFGHLLTSSNYNDDEKKVTGGRNGFGAKLCNIFSTKFTVETANKEYKKNFKQTWAVNMSKASDPKIKDFT-GEEYTKITFSPDLAKFGMDCLDRDTVDLLSRRAYDVAASSTGVKVYLNGKRVPVKSFKDYVELYIKDRTDDAGAPLKVVYEKPGPRWEVAVTISEKGFQHMSFVNSIATTKGGRHVDYITNMIVNSLTETLSKKNKSGVQIKNFQIKNHLWVFVNALIENPTFDSQTKENMTLQQKSFGSKCTLSDKFVAAVVKSGIVETVMAWAKFKQQAQLEKKCAGKKTSKLKGIPKLEDANDAGTRSSSECTLILTEGDSAKTLAVAGLGVVGRDKYGVFPLRGKVLNTREATVKQMMENAEINHIIQIVGLQYKKKYETVDDLKTLRYGKLMIMTDQDQDGSHIKGLIINFIHHNWPSLLRLNFLEEFITPIVKATKGKEEISFYSLPEYEEWKSNKDNWHTYKIKYYKGLGTSTSKEAKEYFSDMVRHRIKFKYEGDQDDRSIQMAFSKKAADQRKEWLTAWMLECKRRRELGLPEIYLYEKNTHSVNYSDFVNKELVLFSNADNERSIPSLVDGFKPGQRKVMFTCIKRNDKREVKVAQLAGSIAEHTAYHHGEVSLMSTIINLAQNFVGSNNINLLEPIGQFGTRLMGGKDAASPRYIFTKLSPLARALIHPHDDPLLKYLQEDNQKIEPEWYMPIIPLVLVNGAEGIGTGWSTKIPNYNPREIVDNIRKMIRGEEPKDMLPWFKNFRGTVEQLDATRYLCSGEVARLEDNQYEISELPIRTWTQSYKENVLE-ILQGNDKIPPIISDYREYNTDTTVRFVLNVLDNQVRHVEAE-GPHKIFKLQSSMSINTMVLFDANGVLKRYNSVMDVLQEFYELRLKFYEKRKLYMVGMLKAEANKLSNQARFIKEKCEGKLVVENKKRKDMINELITKGYESDPVKAWKKSQSREAAVEDEAAEESDAEEEQTDDAADFDYLLGMPMWSLTKERKEDLLKKEQEKKHELETLQAKSESDLWNHDLDVFIEELDKVEAKEREEERV--AKENLASKDKKAGGKKKKKYVDDALLEVMPSPMGERIQPRIDAEMRKKAEKSAAAGAAKAGRPKKRDNPDDDHDDLD---DLVSANTGKSLASRLGTSPD--LVKKRKAGRPTKKNGDGLKQTKINFPKKGKNPWS-SDSDADGGNS-------DSDLSTTNIETVPRSRAVEGKRAAAAKAKYNFSDDDEDDDDDIESLHSNDGVD----ERHVGPEKLSF-LDQTEDN----AAPDAPSPPKPA-------------------KKSNAPKPKKANAHSDEDIVISDTDSEFGDFQQKKKKPSAAASTKQAASTADGSNASD 1479          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1355946814|gb|PRD20904.1| (DNA topoisomerase 2-alpha [Nephila clavipes])

HSP 1 Score: 1711.43 bits (4431), Expect = 0.000e+0
Identity = 829/1314 (63.09%), Postives = 1038/1314 (79.00%), Query Frame = 0

Query:   50 KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGGLEKDL-----DYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAK--AAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSV--NDKGAKKRGSDSSTPKKRPQS-SPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSD 1353
            K+LSVERIYQKK+QLEHILLRPDTYIGSV PVT+ MW+  EEN  +VN+E+TYVPGLYKIFDEILVNAADNKQRDK+MD I+I+IN EKN+I ++NNG+GIPV  HKDEKMFVP+LIFGHLLTSSN+NDEE KVTGGRNGYGAKLCN+FSTKFTVETAS+  +K+FKQ W++NM+KA+E K+  F+ GSD+T ITF PDL KF M  LD D VA+++RRAYDIA  ++GVKV LN K++ VK+FKDY+  +L    D+ G P+K +++   ERWE+AV +S++G+Q +SFVNSIATTKGGRHVDY++  + S IAEV+KKKNKGG  +KP QI++HLW+F+NCLI NPTFDSQTKENMTL +  FGSKC  T KF  Q  K GVVES+++W +FKA  E++ K S KK +KLKGI KLEDANDAGTKNSIDCTLILTEGDSAK+LAV+GLGVVGRDK+GV+PL+GK+LNVREA+HKQI+EN EIN ++KI+GL YKKKY ++DDLK+LRYG+LMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKA+KG     F+SLPE  EWK+ T NW+ +K+KYYKGLGTST+KEAKEYFSDMKRH+I F +  ++DD+AI +AFSKK +D RKEWLT  MEE KRR+ELGL E+YLY K+T  V+Y+DF+NKEL+LFSN+DNERSIPS +DGFKPGQRKV+FTCLKRNDKREVKVAQL GSV E SAYHHGEA+L GTII LAQ+FVGSNNIN+LLP+GQFGTRL GGKD ASPRYIFT ++ +++ +F+  D  +L+YL DDN KIEPE+Y+PIIPMVLVNGA+GIGTGW TK+PNYNPR++V+N+  ++DG +P  M PW+K+F+G I+ +++Q ++V+GEIA +  NKIE+TELP+R+WTQ YKE V+EPM++G+EKIPA I DYKEYHTD TV+FVV M+ +K  QA  ++G H+ FKLQT+IS SSMVLFD  GCLRKY++  +++REF+ +R   YE+RK Y+ G+LEAESLKL NQARFILEK +  + +EN++R  +I+ LQ +G+DSDPVK WK AQK   D +  SE   EK++     DYDYLLGM +WSLT+ERKD+LL+++DEK +EL  L+  S  DLWR DLKEFME+LD+VE ++ EDE  + S + +K   GKKK   E  PSP G R+ P I ++ K K  K      + +KG K    ++KE +          D KD+  SLS++L   V     GAK      +  KKR    SPK   + PW D   ++E  + +   D ++ G D
Sbjct:   24 KKLSVERIYQKKTQLEHILLRPDTYIGSVEPVTQTMWVY-EENVGLVNREVTYVPGLYKIFDEILVNAADNKQRDKSMDLIRIEINPEKNKICIWNNGQGIPVVEHKDEKMFVPSLIFGHLLTSSNYNDEERKVTGGRNGYGAKLCNIFSTKFTVETASKAYKKQFKQTWKDNMTKAEEPKINPFN-GSDFTCITFYPDLAKFKMTVLDKDIVAIMTRRAYDIAGCARGVKVILNDKKLPVKSFKDYISLYLSEKTDDNGDPLKPVHDVVNERWEIAVTLSNSGYQHVSFVNSIATTKGGRHVDYITDQLTSKIAEVIKKKNKGGKDVKPFQIKSHLWVFVNCLIENPTFDSQTKENMTLQIKNFGSKCQLTEKFINQAMKCGVVESIMSWIRFKAQTELEKKCSGKKHSKLKGIPKLEDANDAGTKNSIDCTLILTEGDSAKSLAVSGLGVVGRDKYGVFPLKGKMLNVREASHKQILENAEINNIIKIMGLQYKKKYESIDDLKTLRYGRLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKASKGKEEKCFYSLPEFEEWKTETPNWNKFKVKYYKGLGTSTAKEAKEYFSDMKRHRIRFHYTGNEDDNAIQLAFSKKMVDMRKEWLTNGMEERKRRRELGLPELYLYGKNTTAVTYNDFVNKELILFSNMDNERSIPSAIDGFKPGQRKVLFTCLKRNDKREVKVAQLVGSVAEQSAYHHGEAALAGTIIGLAQDFVGSNNINVLLPVGQFGTRLQGGKDAASPRYIFTMLNSVSKHLFSGLDSPLLHYLYDDNLKIEPEYYVPIIPMVLVNGAEGIGTGWSTKVPNYNPRDLVKNIYKLLDGEDPLPMNPWYKNFRGQIDRLDDQHFLVSGEIAILENNKIEITELPIRTWTQQYKENVLEPMVHGTEKIPAFITDYKEYHTDTTVRFVVTMTPEKFNQAR-EQGFHKVFKLQTSISLSSMVLFDHLGCLRKYDSPEQVLREFYEVRFALYERRKEYMVGVLEAESLKLKNQARFILEKIENKISIENRRRAEMIKTLQDRGYDSDPVKAWKEAQKENIDEEKASESDDEKEIEPSGPDYDYLLGMTLWSLTRERKDDLLKKRDEKIKELDDLKSKSPSDLWRNDLKEFMEELDKVEKEQKEDE--VGSSKLKKKTTGKKKFADEDKPSPFGERINPQIDDDYKKKWEKKPTVPGEVKKGRKKKLPDIKEEDG--------DDIKDV--SLSDRLNTPVKKGKGGAKTAPKQPAGEKKRGAGKSPKKPKRNPWSDDSESNEELSGIS--DMEELGDD 1320