BLAST of hypothetical protein DAPPUDRAFT_329527 vs. L. salmonis genes
Match: EMLSAG00000009984 (supercontig:LSalAtl2s:LSalAtl2s653:17916:32674:1 gene:EMLSAG00000009984 transcript:EMLSAT00000009984 description:"maker-LSalAtl2s653-augustus-gene-0.25")

HSP 1 Score: 1846.63 bits (4782), Expect = 0.000e+0
Identity = 919/1313 (69.99%), Postives = 1085/1313 (82.64%), Query Frame = 0

Query:   44 AGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDET--GSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIP-AIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGG-----LEKD----LDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGA-KGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKK---PWE-DSDATSESD 1339
            A GG  KRLSVERIYQKK+QLEHILLRPD+YIGSV P+TE M++++E   ++VNK+IT+VPGLYKIFDEILVNAADNKQRD  MDTIK+DI+ EKNEI+VFNNGRGIPV  HKDEKMFVPT+IFGHLLTSSNFNDEEEKVTGGRNG+GAKLCNVFS+KFTVETAS+E +K FKQVW  NMSKA E K+  +S G DYTR+TF+PDL KF+M SLD +TVALLSRRAYD+AASSKGVKVFLNGKRI +K FKDYVDFF+K +E+E    S +K ++EA G RWEVA AV +NGF+Q SFVNSIATTKGGRHVD+V+ +I   + E +KKKNKGG++IKP QI+NHLW+F+N LIVNPTFDSQTKENMTL +  FGSKC  + KF   + KSG+VE+VLAWSKFK DQ +K   S KKT+KLKGI KLEDANDAGT+NSIDCTLILTEGDSAKTLA AGL VVGRDKFGVYPLRGKLLNVREATHKQ++ENKEINE+VKI+GLTYKKKY T DD+KSLRYGK+MIMTDQDQDGSHIKGLLINFIHHNWP+LL+ PFLEEFITPIVKA+KG  S  F+SLPE  EWK+ T NW ++KIKYYKGLGTSTSKEAKEYFSDM+RH+ILF ++  QDDH+I MAFSKKA+++RKEWLT WME+CKRRKELG++E+YLYEK+T++V+YS+F+NKELVLFSNLDNERSIPS+VDG KPGQRKV+FTCLKRNDKRE+KVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNN+NLLLPIGQFGTRLAGGKDHAS RYIFTQ++P+TR +F+ +DD VL YL DDN ++EPE+Y+PI+PMVL+NGADGIGTGWMTK+PNYNP++I++N+ ++I+G E ++M PWFK FKG IE I  Q+YVVNGE+A++S+ KIE++ELPV++WTQ+YKE VME MLNGSEK P +II DYKEYHTDVTVKFVV M++DKLR+AE  KGLHQFFKLQTTISTSSMVLFDA GCL+KY N LEI+ EF+ +R+ FYEKRK YL GMLEA++LKL NQARFI  KC+GSLVVENKKRK +I +L  KGFDSDPVK WK  Q+++ D++  +E G     +E+      DYDYL+ MAMWSLT+ERKDELLR+  EK  EL  ++ T ++D+W+ DLKEF  K DE+  KE ED   + + E  KG  KG  K KI    SP GIR+VP I +EL+ K AKAAAAK RK +K     +K  + V DEFD M   KD N  L +K    ++ KG  K  S  S     P+     KGKK   PWE DS +  E D
Sbjct: 1714 AKGGQKKRLSVERIYQKKTQLEHILLRPDSYIGSVQPLTESMYVIEE--GKVVNKDITFVPGLYKIFDEILVNAADNKQRDPKMDTIKVDIDAEKNEIKVFNNGRGIPVVQHKDEKMFVPTMIFGHLLTSSNFNDEEEKVTGGRNGFGAKLCNVFSSKFTVETASKEYKKSFKQVWGANMSKASEPKITPYS-GEDYTRVTFSPDLEKFHMSSLDPNTVALLSRRAYDVAASSKGVKVFLNGKRIPIKGFKDYVDFFIKCYEEENPGASEIKPVFEACGPRWEVAXAVXENGFKQXSFVNSIATTKGGRHVDHVTDLITKALGETIKKKNKGGISIKPFQIKNHLWIFVNALIVNPTFDSQTKENMTLQVKSFGSKCVLSDKFNNLINKSGIVEAVLAWSKFKQDQMLKKNTSGKKTSKLKGIPKLEDANDAGTRNSIDCTLILTEGDSAKTLAXAGLSVVGRDKFGVYPLRGKLLNVREATHKQLLENKEINELVKIMGLTYKKKYETDDDMKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPTLLKRPFLEEFITPIVKASKGQSSFPFYSLPEFEEWKNSTPNWPSFKIKYYKGLGTSTSKEAKEYFSDMRRHRILFSYDGQQDDHSIQMAFSKKAVEERKEWLTNWMEDCKRRKELGMAEVYLYEKNTRSVTYSEFVNKELVLFSNLDNERSIPSLVDGLKPGQRKVVFTCLKRNDKREIKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNVNLLLPIGQFGTRLAGGKDHASARYIFTQLNPMTRYLFHIHDDYVLKYLRDDNLRVEPEYYVPILPMVLINGADGIGTGWMTKVPNYNPKDIIKNIKSLINGGEMKEMVPWFKHFKGSIEYIGEQKYVVNGEVANLSDTKIEISELPVKTWTQTYKESVMEQMLNGSEKNPSSIISDYKEYHTDVTVKFVVTMASDKLREAE-RKGLHQFFKLQTTISTSSMVLFDAYGCLKKYNNPLEIISEFYDVRIRFYEKRKKYLVGMLEADNLKLKNQARFIKMKCEGSLVVENKKRKDIILDLTNKGFDSDPVKCWKKVQEIDCDTEEQTEEGDDTINVEQSDDEKKDYDYLMSMAMWSLTKERKDELLRKAQEKQDELDAVKATLEKDMWKKDLKEFENKYDELMKKEKEDLAKLYAVE--KGVRKGGAKSKISTQASPHGIRIVPRIDDELRTKVAKAAAAKDRKAKKDKNPVVK--DEVPDEFDDMVTGKDGNEKLVDK----ISKKGVVKSSSRKSDSLSSPK-----KGKKRENPWESDSGSGGEED 3009          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|13959708|sp|O42130.2|TOP2A_CHICK (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme)

HSP 1 Score: 1598.56 bits (4138), Expect = 0.000e+0
Identity = 792/1300 (60.92%), Postives = 981/1300 (75.46%), Query Frame = 0

Query:   42 PKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ--KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILS--------KESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKR--PQSSPKTKGKKPW 1329
            PKA G   KRLSVERIYQKK+QLEHILLRPDTYIGSV  VT++MW+ DE+   +  +++T+VPGLYKIFDEILVNAADNKQRDK+M  IK+ I+ E N I V+NNG+GIPV  HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETA RE +K FKQ W +NM KA E  LK F  G DYT +TF PDL KF M  LD D VAL+SRRAYDIA S+K VKVFLNGKR+ VK F+ YVD +LK   DETG+ +K I+E    RWEV + +S+ GFQQ+SFVNSIATTKGGRHVDYV+  I + + +V+KKKNK GV +KP Q++NH+W+F+N LI NPTFDSQTKENMTL    FGS C  + KF       G+VES+L W KFKA  ++  K S+ K  K+KG+ KL+DANDAG+KNSIDCTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y   + LKSLRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR  FLEEFITPI+K +K      F+S+PE  EWKS T N++++KIKYYKGLGTSTSKEAKEYF+DM RH+I FK+   +DD AI +AFSKK +++RKEWLT +ME+ ++R   GL E YLY KDT  ++Y+DFINKELVLFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVK AQLAGSV EMS+YHHGEASLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPL RL+F   DD VL +L DDNQ++EPEWYMPIIPMVL+NGA+GIGTGW  KIPN++ RE V N+  ++DG EP  M P +K+FKG I+ +   +YV++GE++ +    IE+TELPVR+WTQ+YKEQV+EPMLNG+EK P +I DYKEYHTD TVKFVV+MS +KL +AE   GLH+ FKLQT ++ +SMVLFD  G L+KYE+  +I++EFF LRL +Y  RK +L GML AES KL+NQARFILEK DG +V+ENK +K LI+ L ++G++SDPVK WK  Q  + EE  +S  E       D++YLL M +W LT+E+KDEL +++D K +EL  L+  S  DLW+ DL  F+E+LD VEAK+++DE   ++         +     K  K    E +PSP GIRVVP +  E+K +A              +K+  K++++ K+E     D+K    S  +K   S+  + A+KR ++  T ++   P    K   + PW
Sbjct:   20 PKADGA-QKRLSVERIYQKKTQLEHILLRPDTYIGSVETVTQQMWVFDED-VGLNCRDVTFVPGLYKIFDEILVNAADNKQRDKSMSCIKVTIDPENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACREYKKLFKQTWTDNMGKAGEMTLKHFD-GEDYTCVTFQPDLSKFKMTILDKDIVALMSRRAYDIAGSTKDVKVFLNGKRLPVKGFRSYVDLYLKDKVDETGNALKVIHEEVNSRWEVCLTLSEKGFQQVSFVNSIATTKGGRHVDYVADQIVTKLIDVVKKKNKNGVGVKPFQVKNHMWIFVNSLIENPTFDSQTKENMTLQAKSFGSTCKLSEKFIKGAVGCGIVESILNWVKFKAQTQLNKKCSAVKHTKIKGVPKLDDANDAGSKNSIDCTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKMLNVREASHKQIMENAEINNIIKIVGLQYKKNYEDRESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKVSKNKEEIPFYSIPEFEEWKSSTQNYNSWKIKYYKGLGTSTSKEAKEYFADMARHRIGFKYSGPEDDAAITLAFSKKKVEERKEWLTNFMEDRRQRNVHGLPEDYLYGKDTNYLTYNDFINKELVLFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKGAQLAGSVAEMSSYHHGEASLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPVDDNVLRFLYDDNQRVEPEWYMPIIPMVLINGAEGIGTGWSCKIPNFDIRETVNNIRCLLDGKEPLPMLPSYKNFKGTIDELGPNQYVISGEVSILDSTTIEITELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKMSEEKLAEAEA-VGLHKVFKLQTNLTCNSMVLFDHVGFLKKYESPQDILKEFFELRLRYYGLRKEWLIGMLGAESAKLNNQARFILEKIDGKIVIENKPKKELIQVLIQRGYESDPVKAWKELQNKEEEEGDESGEESAAATGPDFNYLLNMPLWYLTKEKKDELCKQRDNKDKELEDLKHKSPSDLWKEDLAAFVEELDAVEAKQMQDEMAGITGKPLKVKGGKQGGKQKVTKAQLAEVMPSPHGIRVVPRVTAEMKAEA--------------EKRIKKKIKSEKNE----SDEKQEGNSSGDKEPSSLKQRLAQKRKAEQGTKRQTTLPFKPIKKMKRNPW 1297          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|135995|sp|P15348.1|TOP2_DROME (RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II)

HSP 1 Score: 1576.99 bits (4082), Expect = 0.000e+0
Identity = 752/1176 (63.95%), Postives = 923/1176 (78.49%), Query Frame = 0

Query:   48 GAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDK-GLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKM------------------------EEDSDSDSEGGLEKDLD---YDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLD 1195
            G K LS+E++YQKKSQLEHILLRPD+YIGSV    E MW+ D    R+V KEI++VPGLYKIFDEILVNAADNKQRDK+M+TIKIDI+ E+N + V+NNG+GIPV MHK++KM+VPT+IFGHLLTSSN+ND+E+KVTGGRNGYGAKLCN+FST FTVETA+RE +K FKQ W NNM KA + ++KDF+ G+DYTRITF+PDL KF M+ LD D VAL+SRRAYD+AASSKGV VFLNG ++GV+NFKDY+D  +K  +D++G P+K ++E A ERWEVA   SD GFQQ+SFVNSIAT KGGRHVD+V   +   + EVLKKKNKGG+ IKP Q+RNHLW+F+NCLI NPTFDSQTKENMTL    FGSKC+ + KF   + KSG+VESVLAW+KFKA  ++  K   +K++K+KGI KLEDAN+AG KNSI CTLILTEGDSAK+LAV+GLGV+GRD +GV+PLRGKLLNVREA  KQ+ EN EIN + KIIGL YKKKY T DDLK+LRYGK+MIMTDQDQDGSHIKGLLINFIH NWP LLRLPFLEEFITPIVKATK +   SF+SLPE  EWK+ T N HTY IKYYKGLGTSTSKEAKEYF DM RH+ILFK++   DD +I MAFSKK I+ RK WLT  M+E KRRKELGL E YLY K TK+++Y+DFIN ELVLFSN DNERSIPS+VDG KPGQRKVMFTC KRNDKREVKVAQL+GSV EMSAYHHGE SL  TI+NLAQNFVG+NNINLL P GQFGTRL+GGKD AS RYIFT MSPLTRLI++  DD +L+Y  DD QKIEP WY+PIIPMVLVNGA+GIGTGW TKI NYNPREI++NL  MI+G EP  M PW+K+F G +E + + RY+  G I  +S N++E++ELPV  WTQ+YKE V+EP+ NG+EK+  II +Y+EYHTD TV+FV+  +  +  +   ++ G ++ FKL TT+ST+ M  FD   CLR++   ++I++E++ LR  +Y +R+++L G L A++ +LS+QARFILEKC+  LVVENK+RKA+ +EL ++G+  DPVK+W+   KM                        +++ + D E   +K  D   +DYLLGM+MW LT+E+K+ELL+++D K  EL  LR  + E LW  DL     KL+
Sbjct:    4 GNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFN-GTDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDDSGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNKGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESVLAWAKFKAQNDIA-KTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDALESKLN 1177          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|6175065|sp|Q23670.2|TOP2_CAEEL (RecName: Full=DNA topoisomerase 2 top-2; AltName: Full=DNA topoisomerase II)

HSP 1 Score: 1514.98 bits (3921), Expect = 0.000e+0
Identity = 736/1246 (59.07%), Postives = 936/1246 (75.12%), Query Frame = 0

Query:   46 GGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEK--MWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVS-KMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEE---------DSDSDSEGGLEKD--------------LDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQK-------GAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRK 1258
            GGG+K++++E IYQKKSQLEHILLRPDTYIGSV   TEK  MW+ + E  ++  ++I+YVPGLYKI+DEILVNAADNKQRD  M+TIKI IN+EKNEI V+NNG+GIPV  HK EK++VP LIFG LLTSSN+ND+E+KVTGGRNGYGAKLCN+FSTKFT+ET+SR+ +  FKQ W  NM++ +E K+   S+  D+T+ITF+PDL KF M+ LD D   L++RRAYD+A SSKGV VFLNGKRI +K F+DYV  +     +E G P+K  YE  G+RW+VA+A+S+ GFQQ+SFVNSIATTKGGRHVDYV+ +M+   I  + +K  K  + IKP QI+NH+W+F+NCLI NPTFDSQTKE MTL   +FGS C  + KF       G+ ++V++W +FK   ++  K S  KT+KLKGI KLEDANDAGTKNS  CTLILTEGDSAKTLAV+GL VVGRDK+GV+PLRGKLLNVRE   KQI +N E+N M+KI+GL YKKKY T DD K+LRYGKLM+M DQDQDGSHIKGL+INFIHH WPSL++  F+EEFITPIVKATKG    SF+SLPE  EW+ +TDNW +YKIKYYKGLGTSTSKEAKEYF DM RH+I FK+    DD+A++MAFSKK I++RK+WL+ WM E K RK+ GL+E YLY KDT+ V++ DF+N+ELVLFSNLDNERSIP +VDGFKPGQRKV+F C KR DKREVKVAQLAG+V E+SAYHHGE SLMGTI+NLAQ++VGSNNINLLLPIGQFGTRL GGKD AS RYIFTQ+SP+TR +F ++DD VL +L ++NQ+IEPEWY PIIPMVLVNGA GIGTGW T IPNYNPRE+V+N+  +I G   + + PW+K+F+G I  I+  R+   GE+A + +N IE+TELP++ WTQ YKE+V+E ++  S+K   +I DYKEYHTD TVKFVV++S  KLR+ E  + LHQ FKLQ  I+T+ MVLFDA GCLR Y +   I +EF+  R   Y +RK YL G+L+A+S +L+NQARFIL K +  +V+ENKK+ A+++ L +  FD+DPVKKWK  QK++E         D D  +   +E+D               DYDYL+GMA+  L++E K++L++  +EK  E+R++   + +DLW  DL  F+ +LD+ EA+E  D+   +   ++K       G   KK +  E LPS  G R+ P++    K K  K +  K  +
Sbjct:   55 GGGSKQMAIEDIYQKKSQLEHILLRPDTYIGSVEH-TEKTPMWVYNMEESKLEQRDISYVPGLYKIYDEILVNAADNKQRDPKMNTIKITINKEKNEISVYNNGKGIPVTQHKVEKVYVPELIFGTLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTKFTLETSSRDYKSAFKQTWIKNMTRDEEPKIVK-STDEDFTKITFSPDLAKFKMKELDDDICHLMARRAYDVAGSSKGVAVFLNGKRIPIKGFEDYVQMYTSQFNNE-GEPLKIAYEQVGDRWQVALALSEKGFQQVSFVNSIATTKGGRHVDYVADQMVAKFIDSIKRKLTKTSMNIKPFQIKNHMWVFVNCLIENPTFDSQTKETMTLQQKQFGSTCVLSEKFSKAASSVGITDAVMSWVRFKQMDDLNKKCSKTKTSKLKGIPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPLRGKLLNVREGNMKQIADNAEVNAMIKILGLQYKKKYETEDDFKTLRYGKLMVMADQDQDGSHIKGLVINFIHHFWPSLIQRNFVEEFITPIVKATKGKEEVSFYSLPEYSEWRMNTDNWKSYKIKYYKGLGTSTSKEAKEYFLDMVRHRIRFKYNGADDDNAVDMAFSKKKIEERKDWLSKWMREKKDRKQQGLAEEYLYNKDTRFVTFKDFVNRELVLFSNLDNERSIPCLVDGFKPGQRKVLFACFKRADKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSNNINLLLPIGQFGTRLQGGKDSASARYIFTQLSPVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGIGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVAVLDDNTIEITELPIKQWTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLRELERGQDLHQVFKLQAVINTTCMVLFDAAGCLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILAKINNEIVLENKKKAAIVDVLIKMKFDADPVKKWKEEQKLKELRESGEIELDEDDLAAVAVEEDEAISSAAKAVETKLSDYDYLVGMALIKLSEEEKNKLIKESEEKMAEVRVIEKKTWQDLWHEDLDNFVSELDKQEAREKADQDASIKNAAKKLAADAKTGRGPKKNVCTEVLPSKDGQRIEPMLDAATKAKYEKMSQPKKER 1297          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|12644105|sp|P08096.2|TOP2_SCHPO (RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II)

HSP 1 Score: 1190.64 bits (3079), Expect = 0.000e+0
Identity = 659/1495 (44.08%), Postives = 909/1495 (60.80%), Query Frame = 0

Query:   58 YQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLP-FLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEED-----SDSDSEGGLEKDLD---YDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEK----LDEVEAKEIEDERVI--LSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKR----GSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGSEVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAP-AHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKKPP--------------AAATKKSKKTSQGSDYDDSDEDDMFKDS 1518
            YQ+ +  EH+L RPDTYIGS+ P T +MW+ D E  ++  K +TYVPGLYKIFDEI+VNAADNK RD NM+T+K+ ++ E N I ++NNG+GIP+E+H  EK+++P LIFG+LLTSSN++D ++KVTGGRNGYGAKLCN+FST+F VETA +E  K++KQ W +NMS+  E  +       +YT+ITF PDL KF M+ +D D V+++ RR YD+A + +  KV+LN +RI +  FK YV+ +L         P + IYE   +RW+VA AVSD  F+Q+SFVN+I+T +GG HV+YV+  I   I EV+KK+NK    +K  QI+N++ +F+NC I NP+FDSQTKE +T  ++ FGS+C+ + KF   ++KS VVE VL ++  KADQ++ +K      +++ G+ KLEDAN AGTK S  C LILTEGDSAK+LAV+GL VVGRD +GV+PLRGKLLNVREA+H QI+ NKEI  + KI+G T+KK Y+   D+K LRYG LMIMTDQD DGSHIKGL+IN++  ++PSLL++P FL +FITPI+K T+G+   +F++LPE   WK   +N   +KIKYYKGLGTS   + K YFSD+ RH   F    ++D   I MAF+KK  D RKEWL T+              IY+ +     +   DFIN+EL+ FS  DN RSIPSVVDG KPGQRKV++ C KRN   E KV++LAG V   +AYHHGE S+  TI+NLAQNFVGSNNINLL+P GQFGTR  GGK+ ++ RY+ T +SPL R++FNSNDD +LNY  D+ Q IEPE+Y+PI+PMVLVNGA+GIGTGW T IPNYNP++I  NL +M++G   E M PW++ F+G I  +   RY ++G I  I ENK+E+TELP+R WTQ  KE  +E  L G+EKI   I DY+ +H +  V F V ++   +++A L++ L   FKL  T +TS+M+ FDA G ++KY++V +I+ EF+ +RL  Y++RK ++   LE    + SNQARFI    +G LVV  KK+K LI EL+ K F   P+ K K    ++ +     ++ +  G + +D D   Y+YLL M +WSLT ER  ELL++KDE   EL  L   + ++LW  DL  F       +D+++ + +E+E+        ++K  +GK            G R    IA    + A K  A+   K    ++K    +E    +      D ++ ++     G+SV +    K      SDS   +KR QS                          D +D+GS                  +P  KIA              S + + ++   P A  +F  DD D+     LKPS   + + S+                K K +  S  KK+  + DDD  I    +  PKAS    +PP              AAAT K+      S +D  DE  M  DS
Sbjct:   78 YQRLTPREHVLRRPDTYIGSIEPTTSEMWVFDSEKNKLDYKAVTYVPGLYKIFDEIIVNAADNKVRDPNMNTLKVTLDPEANVISIYNNGKGIPIEIHDKEKIYIPELIFGNLLTSSNYDDNQKKVTGGRNGYGAKLCNIFSTEFVVETADKERMKKYKQTWYDNMSRKSEPVITSLKKPDEYTKITFKPDLAKFGMDKIDDDMVSIIKRRIYDMAGTVRETKVYLNNERISISGFKKYVEMYLASDTKPDEEPPRVIYEHVNDRWDVAFAVSDGQFKQVSFVNNISTIRGGTHVNYVANKIVDAIDEVVKKENKKA-PVKAFQIKNYVQVFVNCQIENPSFDSQTKETLTTKVSAFGSQCTLSDKFLKAIKKSSVVEEVLKFATAKADQQL-SKGDGGLRSRITGLTKLEDANKAGTKESHKCVLILTEGDSAKSLAVSGLSVVGRDYYGVFPLRGKLLNVREASHSQILNNKEIQAIKKIMGFTHKKTYT---DVKGLRYGHLMIMTDQDHDGSHIKGLIINYLESSYPSLLQIPGFLIQFITPIIKCTRGNQVQAFYTLPEYEYWKEANNNGRGWKIKYYKGLGTSDHDDMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEWLRTYR-----------PGIYM-DYTQPQIPIDDFINRELIQFSMADNIRSIPSVVDGLKPGQRKVVYYCFKRNLVHETKVSRLAGYVASETAYHHGEVSMEQTIVNLAQNFVGSNNINLLMPNGQFGTRSEGGKNASASRYLNTALSPLARVLFNSNDDQLLNYQNDEGQWIEPEYYVPILPMVLVNGAEGIGTGWSTFIPNYNPKDITANLRHMLNGEPLEIMTPWYRGFRGSITKVAPDRYKISGIINQIGENKVEITELPIRFWTQDMKE-YLEAGLVGTEKIRKFIVDYESHHGEGNVHFNVTLTEAGMKEA-LNESLEVKFKLSRTQATSNMIAFDASGRIKKYDSVEDILTEFYEVRLRTYQRRKEHMVNELEKRFDRFSNQARFIHMIIEGELVVSKKKKKDLIVELKEKKF--QPISKPKKGHLVDLEVENALAEEEQSGDVSQDEDSDAYNYLLSMPLWSLTYERYVELLKKKDEVMAELDALIKKTPKELWLHDLDAFEHAWNKVMDDIQREMLEEEQSSRDFVNRTKKKPRGKS----------TGTRKPRAIAGSSSSTAVKKEASSESKPSTTNRKQQTLLEFAASKEPEKSSDINIVKTEDNSHGLSVEENRISKSPGLDSSDSGKSRKRSQSV-------------------------DSEDAGSK-----------------KPVKKIAA-------------SASGRGRKTNKPVATTIFSSDDEDDLLPSSLKPSTITSTKASA----------------KNKGKKASSVKKQSPEDDDDDFIIPGSSSTPKASSTNAEPPEDSDSPIRKRPTRRAAATVKTPIYVDPS-FDSMDEPSMQDDS 1469          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|7388329|sp|Q9Y8G8.1|TOP2_PENCH (RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II)

HSP 1 Score: 1130.93 bits (2924), Expect = 0.000e+0
Identity = 577/1160 (49.74%), Postives = 778/1160 (67.07%), Query Frame = 0

Query:   50 KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKA-----IYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLP-FLEEFITPIVKATKG-----SLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGGLEKDL-------DYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFM 1191
            K  +V   YQK +QLEHI+ RPDTY+GS    T+ MW+   E++ +  +E++YVPGLYKIFDEI+VNAADNKQ DKNMD I++ I+RE  EI V+NNGRGIP+EMH  EKM+VP LIFGHLLTSSN++D + KVTGGRNG+GAKLCNVFST+FT+ET     +K++KQ W  NM+K  +AK+ D + G+DYT++TF PD  KF M  +D D  AL+ RR YD+A ++K V V LNG RI ++NFK Y++ + K    E G    A     I  +   RWEV  AVSD  FQQ+SFVNSIA T GG HV+Y++  I S +AE +KKKNK G T+KP QIRNH+++F+N LIVNP F SQTKE +T   ++FGSKC     F+ ++ K+ V+ ++L +++ KADQ +K     +++       KL DAN AGTK    CTLILTEGDSAK LA+AG  VVG D FGV+PLRGKLLNVR+A+  QI +N+EI  +   IGL +KK+Y+   D + LRYG LMI TDQD DGSHIKGLLINF+   +PSLL++P FL EFITPIVK  KG     +   SFF++PE   WK    +   ++ KYYKGLGTST+++A+ YF D+ RH   F    D +   I +AFSKK  D+RK  +  +                  +     ++Y+DFINKEL+LFS  DN RSIPSVVDG KPGQRKV++TC +RN K+++KV +LAG V   +AY HGE SL  TI+ LAQ FVGSNN+N L P G FG+RL GG D AS RYI+T++ P  R +F+++DD +L Y  DD  KIE E Y+P++PM+L+NGADGIGTGW +  PNYNP +IV NL  M+DG   + M+PWF+ F G + ++   R+  +G I    + ++E+TELP+R+WTQ +K++ +E ++  +EK P+ I+DYK+Y+T   V FV+QM    ++ A + +GL + FKL  T++T+++V FD +  + KY +V +IM+EF+ +RL +YE+RK Y    ++ E  KLSNQARF+    DG LV+  KK+  L+ EL+ K F   P  K K A K  E      E    +          YDYLLGM +WSLTQER ++L R+  +K  E+  L   SKED+W+ DL++F+
Sbjct:   60 KDTNVSEKYQKLTQLEHIIKRPDTYVGSTERTTQHMWVYSSESEGMEYREVSYVPGLYKIFDEIVVNAADNKQNDKNMDEIRVTIDRESGEISVWNNGRGIPIEMHSKEKMYVPELIFGHLLTSSNYDDTQMKVTGGRNGFGAKLCNVFSTEFTIETQDSRQKKKYKQTWTENMTKMGKAKITD-AKGADYTKVTFKPDYAKFGMTGMDDDFEALVKRRVYDLAGTAK-VAVKLNGSRIPIRNFKMYMEMYTKAIRRERGDMGPAGKNEIITCSPDPRWEVGFAVSDGAFQQVSFVNSIAITSGGTHVNYIADQICSKLAEQVKKKNKNGATLKPAQIRNHIFIFVNALIVNPAFTSQTKEQLTTKQSQFGSKCVLEEDFYKKILKTEVMTNILHFAQQKADQMLKKTDGGRRSRMNNP--KLTDANKAGTKEGHHCTLILTEGDSAKGLAMAGRAVVGPDLFGVFPLRGKLLNVRDASFDQIAKNQEIQNIKNFIGLQHKKEYT---DTRGLRYGHLMITTDQDHDGSHIKGLLINFLQAQFPSLLKIPEFLIEFITPIVKVWKGDPKNPTKQRSFFTMPEYEAWKEEHGHERGWEHKYYKGLGTSTTEDAQIYFRDLDRHLKEFHTMQDNEVELIELAFSKKKADERKGNVRQFRPGT------------FLDHSVDKITYTDFINKELILFSMADNIRSIPSVVDGLKPGQRKVLYTCFRRNLKKDMKVVELAGHVSGTTAYQHGEGSLQQTIVGLAQTFVGSNNVNCLEPSGNFGSRLQGGADCASARYIYTRLWPFARRVFHNHDDPLLTYNEDDGAKIEQEVYVPVVPMILINGADGIGTGWSSSTPNYNPEDIVENLKRMMDGEPLKPMQPWFRGFTGEVTAVGQDRFKFSGIIKETGDKEVEITELPIRTWTQDFKDK-LEDIIK-AEKTPSFIKDYKDYNTHTKVHFVIQMDEKHMKTA-IAEGLEEKFKLSKTMTTTNLVAFDPE-RITKYASVEDIMKEFYAVRLKYYERRKQYQLSEMQKELDKLSNQARFVQMIIDGKLVISKKKKAVLVAELKEKDF--KPFPKVKEAVKAGETEPVVEEEEDSESGDTEVLSNSYDYLLGMPIWSLTQERVEKLRRQIGDKELEIDTLIKLSKEDIWKRDLEDFI 1194          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|1174740|sp|P30182.2|TOP2_ARATH (RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II)

HSP 1 Score: 1127.46 bits (2915), Expect = 0.000e+0
Identity = 604/1217 (49.63%), Postives = 819/1217 (67.30%), Query Frame = 0

Query:   32 AKKANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAAS-SKGVKVFLNGKRIGVKNFKDYVDFFLKG-HEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLP-FLEEFITPIVKAT-KGSLST-SFFSLPELHEWK-SHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQR----YVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVK----KWKNAQKMEEDS---------DSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKI 1225
            A  A   AK   A GG     ++E +YQKKSQLEHILLRPDTYIGS+   T+ +W+   E   +V + +TYVPGLYKIFDEILVNAADNKQRD  MD++++ I+ E+N I V N+G G+PVE+H++E ++VP +IFGHLLTSSN++D  +K TGGRNGYGAKL N+FST+F +ETA  +  K++KQV+ENNM K  E  +   +   ++T++TF PDL KFNM  L+ D VAL+S+R +DIA    K VKV LNGK+I VK+F DYVD +L   ++  T  P+  + E   +RWEV V++S+  FQQ+SFVNSIAT KGG HVDYV+  I ++I   + KKNK       H ++NHLW+F+N LI NP FDSQTKE +TL  + FGSKC  +  F  ++ KSGVVE++L+W+ FK ++++K    +K    L  + KL+DA +AG KNS  CTLILTEGDSAK+LA+AG  V+G +  GV+PLRGKLLNVREA+  QI  NKEI  + KI+GL    KY  V+   SLRYG++MIMTDQD DGSHIKGLLINFIH  WPSLL++P FL EFITPIVKAT KG+    SF+S+PE  EWK S   N   + IKYYKGLGTST++E KEYFS++  H+  F  E +QD  AI +AFSKK I+ RK WL++++              +L ++  K V+YSDF+NKEL+LFS  D +RSIPS+VDG KPGQRK++F   K+  ++E+KVAQL G V  +SAYHHGE SL   II +AQ++VGSNNINLLLP GQFGTR +GGKD AS RYIFT++SP+TR++F  +DD +L+YL +D Q+IEP WYMPIIP VLVNGA+GIGTGW T IPNYNPREIV N+  +++G     M PW++ FKG IE   ++     Y + G    + E  I +TELP+R W   YK  +    L      P   QD K Y+ + +V F + +S + +  A   +G  + FKL TTI+TS+M LFD KG ++KY    +I+ EFF LR  +YEKRK  +   +E E LKL N+ARFIL    G ++V  +K+  ++E+L++KGF   P K    +   A  +++D+         D +S        +YDYLL MA+ SLT E+ +ELL  +D+    +  ++ T+ + LW +DL+   ++L++++ K+ + ++ I + + +  AK    +K+
Sbjct:   13 ANVAKAPAKSRAAAGGK----TIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLWVY--EKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGAGVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETADGKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKRVFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVCVSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKA-HNVKNHLWVFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKVGKSGVVENLLSWADFKQNKDLKKSDGAKTGRVL--VEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYCGVFPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVN---SLRYGQMMIMTDQDHDGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKATRKGTKKVLSFYSMPEYEEWKESLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIELAFSKKKIEARKNWLSSYVPGN-----------HLDQRQPK-VTYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFVAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDYVGSNNINLLLPNGQFGTRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQRIEPTWYMPIIPTVLVNGAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGFKGTIEKTASKEGGCTYTITGLYEEVDETTIRITELPIRRWNDDYKNFLQS--LKTDNGAP-FFQDVKAYNDEKSVDFDLILSEENMLAAR-QEGFLKKFKLTTTIATSNMHLFDKKGVIKKYVTPEQILEEFFDLRFEYYEKRKETVVKNMEIELLKLENKARFILAVLSGEIIVNKRKKADIVEDLRQKGFTPFPRKAESVEAAIAGAVDDDAAEEPEEILVDPESSSSYIPGSEYDYLLAMAIASLTIEKVEELLADRDKMIIAVADMKKTTPKSLWLSDLESLDKELEKLDLKDAQVQQAIEAAQKKIRAKSGAAVKV 1201          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|51338747|sp|O93794.2|TOP2_CANGA (RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II [[Candida] glabrata CBS 138])

HSP 1 Score: 1118.99 bits (2893), Expect = 0.000e+0
Identity = 571/1182 (48.31%), Postives = 794/1182 (67.17%), Query Frame = 0

Query:   58 YQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLK----------GHEDETGSPVKAI-YEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLP-FLEEFITPIVKATKGSL---STSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGF-----DSDP---------------------VKKWKNAQKMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVE 1198
            YQK SQLEHIL RPDTYIGSV    ++ WI DEE   +++K +  VPGL+KIFDEILVNAADNK RD +M  I + IN E+N I V N+G+GIP+E+H+ EK+++P LIFGHLLTSSN++D+E+KVTGGRNGYGAKLCN+FST+FT+ETA  +N +++ Q WENNM+     K+  +  G  YT++ F PDL +F MESLD+D + ++ RR YDI  S + V V+LNGK + ++NFK+YV+ +LK          G  D T S +  I YE   +RWE+A AVSD  F+Q+SFVNSIATT GG HV+Y++  I   ++++LKKK K    IKP+QI+N++++FINCLI NP F SQTKE +T  +  FGS+C  +  +  ++ K+ +   +   +   A+  +K    S+K+ ++    KLEDAN AGTK+   CTLILTEGDSA +LAVAGL VVGRD +G YPLRGK+LNVREAT  QI++N EI  + KI+GL ++KKY   +D KSLRYG LMIMTDQD DGSHIKGL+INFI  ++P LL +P FL EFITPIVK T         SFF+LP+  +W+    + +++K KYYKGLGTSTS E  EYFS++  H   F      D   I++AFSKK  D RKEWL        R+ E G     + +     +  SDFINKEL+LFS  DN RSIPSV+DGFKPGQRKV++TC K+N   E KVA LA +V + +AYHHGE +L+ TII +AQNFVG+NNI  L P G FGTR  GGKD A+ RYI+T+++ + R +F+  DD +  Y+ +D + +EPEWY+P++PMVL+NGA+GIGTGW T IP +NP ++V N+ ++++  E +DM PWF+ + G +E IE+QRY + G I  +  N +E+TELP R+WT + KE ++   L GSEK+   I+D +E H + T+KF+++++ +++ +     G ++ FKL + IS  +MV FD +G ++KY++V EI+++F+ +RL +Y+KRK+Y+ G L+ E+ KLS Q +FI    D SL+V NK +K LI EL+  GF     +  P                      +  +  +K ++D +S   G  E    Y+YLLG+ +WSLT+ER ++LL++K EK  EL  L   S +DLW  DL +F+   ++ +
Sbjct:   11 YQKISQLEHILKRPDTYIGSVEIQEQEQWIYDEETDCMIDKTVNIVPGLFKIFDEILVNAADNKVRDPSMKRIDVTINPEENFIEVRNDGKGIPIEIHEKEKIYIPELIFGHLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTEFTLETADPKNGRKYVQTWENNMNVCHPPKITSYKKGPSYTKVAFKPDLSRFGMESLDSDILGVMRRRVYDINGSVRDVNVYLNGKPLKIRNFKNYVELYLKSLEKMRKMDNGESDTTPSNIPTILYERVSDRWEIAFAVSDISFKQVSFVNSIATTTGGTHVNYIADQIVRKVSDILKKKKKN---IKPYQIKNNMFIFINCLIENPAFTSQTKEQLTTRVKDFGSRCDISSDYINKIMKTDLATKIFEIADENANNALKKSDGSRKS-RITDYPKLEDANKAGTKDGYKCTLILTEGDSALSLAVAGLAVVGRDYYGCYPLRGKMLNVREATADQILKNAEIQAIKKIMGLQHRKKY---EDTKSLRYGHLMIMTDQDHDGSHIKGLIINFIETSFPGLLDIPGFLIEFITPIVKVTITKPIKKVISFFNLPDYEKWREEESHKYSWKQKYYKGLGTSTSPEIIEYFSNLDTHLKKFHALQGDDKDLIDLAFSKKKADDRKEWL--------RQYEPGT----VLDPTLNEIPISDFINKELILFSLADNVRSIPSVLDGFKPGQRKVLYTCFKKNLTTEKKVANLAPAVSDYTAYHHGEQALVQTIIGMAQNFVGTNNIYFLKPNGAFGTRATGGKDAAAARYIYTELNKIARKVFHPADDPLFRYVQEDEKTVEPEWYLPVVPMVLINGAEGIGTGWSTSIPPFNPLDVVNNIRHLLNDEEMDDMHPWFRGWTGTMEKIESQRYRMYGRIEQVGPNTLEITELPARTWTSTIKEHLLLG-LGGSEKVKPWIKDMEEQHAE-TIKFIIKLTDEEMTKTR-KLGFYERFKLISPISLQNMVAFDYRGKIKKYDHVHEILKDFYEVRLEYYQKRKDYMTGRLQWEAEKLSFQVKFIKMIIDKSLIVTNKPKKQLISELEELGFPRINKEGKPHFGKIDEEVEAIISEDEDEDLEESEEATRKKDKDDESTVNGPEELFGTYEYLLGLKIWSLTKERYEKLLKQKQEKETELENLLKLSAKDLWNNDLDDFLTAYEDFQ 1170          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|6094496|sp|O24308.1|TOP2_PEA (RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II; AltName: Full=PsTopII)

HSP 1 Score: 1114.75 bits (2882), Expect = 0.000e+0
Identity = 583/1185 (49.20%), Postives = 786/1185 (66.33%), Query Frame = 0

Query:   53 SVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAAS-SKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLP-FLEEFITPIVKATKGSLS-TSFFSLPELHEWKSHTDNWHT-YKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIE-SIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIP-AIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGGLEKDL---------------DYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKG 1216
            ++E +YQKK+QLEHILLRPDTY+GS+   T+ +W+   EN  +V++ ++YVPGLYKIFDEILVNAADNKQRD +MD++K+ I+ E N + V+NNG G+PVE+H++EK++VP LIFGHLLTSSN++D  +K TGGRNGYGAKL N+FST+F +ETA     K++KQV+ NNM    E  +    +  ++T++TF PDL KF M  L+ D VAL+ +R  D+A    K VKV LNG  I  K+F+DY D FLK  E     P+  I+   G+RWE+ +++SD  FQQ+SFVNSIAT KGG HVDY++  I + I   + KK K    +K H ++NHLW+F+N LI NP FDSQTKE +T     FGSKC         ++KSG+V+++L+W+ FK  +++K K    KT +L+G  K EDAN+AG +NS  CTLILTEGDSAK LA+AGL VVGRD +GV+PLRGKLLNVREA+ KQI++N+EI  + KI+GL   K+Y+ V   KSLRYG LMIM DQD DGSHIKGLLINFIH  WPSLL++P FL EF TP+++A+  + + TSF+S+PE   WK    N  T +KIKYYKGLGTST +E +EYFSD+ RH+  F  + + D +AI +AFSKK  + RK W+        R  E G       + + K ++Y DF+NKEL+LFS  D                   +F   K+   +E+KVAQ  G V E SAYHHGE SL  TII +AQ+FVGSNNINLL P GQFGT   GGKDHAS RYI+T++SP+TR +F+ +DD +L YL +D + IEP WYMPIIP+VLVNG++GIGTGW + IPNYNPREI+ N+  +++G E   M PW+K F+G IE S +   Y+VNG +  I E    +TELP+R WTQ YK Q +E + +G+  +   +I+D+++   D  V   ++M  +K+    L +GL + FKL +TISTS+M LFDA+G  +K++   +I+ EFF LRL++YEK K Y+ G L    L L N+ RFIL   +G ++V N+K+  L+ EL+ KGF   P K      ++   +D DSE   + +                DYD LL + + +LT E   +LL  K EK +E  +L GT    LW  DL EF +KLDE++ K  ED+R   S+ S+K 
Sbjct:   18 TIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVY--ENDEMVHRAVSYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQEEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETADGRRLKKYKQVFSNNMGTKCEPVITKCKASENWTKVTFKPDLEKFKMAYLEEDVVALMKKRVLDMAGCFGKTVKVELNGTLIRFKSFRDYADLFLKCAEKSKPMPLPRIHAKVGDRWEICISLSDGQFQQVSFVNSIATIKGGTHVDYITNQITTYIMNKVNKKKKDA-NVKAHTVKNHLWVFVNSLIDNPAFDSQTKETLTTRQASFGSKCDVPESMLKDVEKSGIVDTLLSWADFKQSKDLK-KTDGTKTQRLRGC-KAEDANEAGGRNSEKCTLILTEGDSAKALAMAGLSVVGRDHYGVFPLRGKLLNVREASSKQIMDNEEIQNIKKILGLQQNKEYTNV---KSLRYGHLMIMADQDHDGSHIKGLLINFIHSFWPSLLKVPSFLVEFTTPVIRASHPNKTITSFYSMPEYEAWKERLGNSATSWKIKYYKGLGTSTPQEGREYFSDLGRHRKDFIWDDELDGNAIELAFSKKKAEGRKIWM--------RNFEPGTCR----DHEAKLINYKDFVNKELILFSRAD-------------------LFGSFKKKLYKEIKVAQFIGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLKPNGQFGTCNLGGKDHASARYIYTELSPVTRCLFHEHDDKLLEYLNEDGKSIEPNWYMPIIPLVLVNGSEGIGTGWSSYIPNYNPREIIANVRRLLNGEELVPMDPWYKGFRGTIEKSAKEGGYIVNGTVTEIDEQTFRITELPIRKWTQDYK-QFLESITDGAPNVKDPLIEDFRQNGDDAIVDIEIKMKPEKIATI-LQEGLFKKFKLTSTISTSNMHLFDAEGN-KKFDTPEQILEEFFPLRLDYYEKSKEYILGNLNRLLLILDNKVRFILGVVNGEIIVSNRKKAELLIELKEKGFTPMPRKGKSTKPQVAGANDDDSEEQEDAEPETASQSVSVEGATWGDYDDLLSLPIGTLTLESVQKLLDEKTEKEKEYEILSGTPTTSLWLKDLDEFEKKLDELDLKYAEDDRKRASQGSKKA 1160          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|2501297|sp|P87078.1|TOP2_CANAX (RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II)

HSP 1 Score: 1108.98 bits (2867), Expect = 0.000e+0
Identity = 586/1274 (46.00%), Postives = 817/1274 (64.13%), Query Frame = 0

Query:    4 SEDDFDPMFEANGDTQKANGEAKKANGEAKKANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSP----------VKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGG--VTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPK--FFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLP-FLEEFITPIVKAT------KGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGF-----DSDPVKKWK-----------------------------NAQKMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKK 1222
            SEDDF P        + A+ ++K+  G+A  +   + +        K  +    YQK SQLEHIL RPDTYIGSV     +MW  D E + +V KE+T VPGLYKIFDEILVNAADNK RD +M  I++ I+ E N I V N+G+GIP+EMH  E M++P LIFG+LLTSSN++D+++KVTGGRNG+GAKLCN+FST+F VETA     K +KQ W NNMS   + K+    +  +YT+ITF PDL KF+M+ LD D +++L RR YD+  + K   ++LN KR+ + +FK YV+ ++K  ++ +  P             ++E   +RWEVA AVSD  F Q+SFVNSIATT GG HV YVS  I + + E L KK KG   + IKP ++R++++LFINCLI NP F SQTKE +T  +++FG K  F        ++ K+ +V+ + A +    D+ ++    S+K+ ++KG + L DAN AGTK+  +CTLILTEG SA  LAVAGL VVGRD +G +PLRGKLLNVREA+  QI +N EIN + +IIGL +KK Y T +++KSLRYG +MIMTDQDQDGSHIKGL+INF+  ++P LL +P FL EFITPIVK T       G     F+++PE   W+        +  KYYKGLGTST  EA+EYF+ + RH   F     +D   I++AFSKK  D+RKEWL  ++       E+  +EI +          SDFINKE +LFS  DN RSIPSV+DGFKPGQRKV++ C K+  + E+KVAQLAG V E + YHHGE SL+ TII LAQNFVGSNNIN+L P G FG+R AGGKD ++ RYIFT++S +TR IFN  DD +  Y+ DD Q +EPEWY+P++PM+LVNGA+GIGTGW T IP+YNP+++V N+  +++G E ++M PW+K + G +E +  Q++ V+G I  I  N +E+TE+PV++WT + KE ++     G+EK    I+D +E+HT  +++FVV+++ D   Q  L  GL + FKL +++S ++MV FD  G ++KY +VLEI+++F+++RL +Y+KRK+Y+   L+ + L LS QARFI    +  L V NKK+K L+  L+   F     D  P+K  +                             N Q+ E +  +     +     YDYLLGMA+WSLT ER   +++++D+K  EL  L   S +DLW  DL EF+ + D+   ++ ++   + S   +K  K + K
Sbjct:   13 SEDDFVPT-SKKSTKKNASSKSKQPLGDATNSTVSSSRSST----PKPTNASETYQKLSQLEHILKRPDTYIGSVEKTKTEMWCFDAETESMVFKEVTIVPGLYKIFDEILVNAADNKIRDPSMKNIRVKIDAENNIIEVMNDGKGIPIEMHTKENMYIPELIFGNLLTSSNYDDDQKKVTGGRNGFGAKLCNIFSTQFEVETADLNMGKLYKQSWTNNMSNVSKPKITTLRTKKEYTKITFRPDLSKFDMDCLDNDLLSVLRRRVYDLCGTVKNCNIYLNDKRLNISSFKGYVEMYVKAIKERSPEPEPQDGTIKNFTTIVHEVFNDRWEVAFAVSDGSFNQVSFVNSIATTSGGTHVKYVSDQIINKLVETLSKKEKGKKKLMIKPQEVRDNMFLFINCLIENPAFTSQTKEQLTTKVSQFGGKDKFVANDNLINRILKTSIVDKIRAIANANEDKALQKADGSRKS-RIKGQVNLVDANKAGTKDGHNCTLILTEGLSAMNLAVAGLSVVGRDYYGCFPLRGKLLNVREASADQISKNAEINSLKQIIGLQHKKVY-TAENIKSLRYGHIMIMTDQDQDGSHIKGLIINFLETSFPGLLDIPGFLLEFITPIVKVTVKARGAGGKRVIPFYTMPEFEHWRDTEGKQCRWTQKYYKGLGTSTPMEAREYFTALDRHLKRFHALQGEDKDYIDLAFSKKKADERKEWLQGFLPGTHLDPEI--TEIPI----------SDFINKEFILFSMSDNVRSIPSVLDGFKPGQRKVLYGCFKKKLRSEIKVAQLAGYVSENTGYHHGEQSLVQTIIGLAQNFVGSNNINVLKPNGSFGSRAAGGKDFSAARYIFTELSEITRKIFNPLDDPLYTYVQDDEQTVEPEWYLPVLPMILVNGAEGIGTGWSTNIPSYNPKDLVTNIRRLMNGEELQEMTPWYKGWGGDLEPMGPQKFKVSGRIEQIDSNTVEITEIPVKTWTNNVKEFLLSGF--GNEKTQPWIKDMEEHHT-TSIRFVVKLT-DAEMQKSLRIGLLERFKLVSSLSLANMVAFDPMGRIKKYNDVLEIIKDFYYVRLEYYQKRKDYMTDNLQNQLLMLSEQARFIKMIIEKQLSVANKKKKQLVALLEEHNFTKFSKDGKPIKSSEELLTGDDADEEEETQEQEGDEDVGNTSVANIQEGEPEQAAHVPETIYS--SYDYLLGMAIWSLTYERFMRIMQQRDQKEAELNALLSKSAKDLWNQDLDEFLAEFDKFLLRDEQERESLASNGKKKSTKRRAK 1261          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|730968|sp|P41001.1|TOP2_PLAFK (RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II)

HSP 1 Score: 1092.03 bits (2823), Expect = 0.000e+0
Identity = 593/1221 (48.57%), Postives = 806/1221 (66.01%), Query Frame = 0

Query:   49 AKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKN-----MDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLK--------------------------------------GHEDETGSPVKA---------------IYEAAGERWEVAVAVSDNG-FQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKH---SSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLP-FLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHH-GEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDS-DPVKKWKNAQKMEE------DSDSDSE---GGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLD 1195
            AK  ++E  YQKKSQ+EHILLRPDTYIGSV   T+ +W+ ++E  R+V K ITYVPGLYKIFDEI+VNAAD K R+K      M  IKI+IN+E   I V+N+G GIPV++HK+  ++VP +IFG LLTS N++D E+++TGGRNG+GAKL N+FS +F V+      +KEFK  W +NMSK  E  +K+++ G DY ++TF PDL KF M  +D D  +LL +R YD+A +   V+V+LNG+R+ VK+FK YVD +LK                                       +E   G+P K                I+E    RWE+ V+ SD   FQQ+SFVNSI TTKGG HV+Y+ + + S++++    KNKGG+ IK   IRNHLW+F+NCLIVNPTFDSQTKE +T    KFGSKC  + K    + KS ++ ++L W++ KA  E+K K    SSK   ++ GI KLEDANDAG+K S +CTLILTEGDSAKT  +AGL +VGRDK+GV+PL+GKLLNVR+A+ KQ+++NKEI  + +I+GL    K    DD+K LRYG LMIMTDQD DGSHIKGLLIN IH  WPSLL+   FL EF+TPIVK  KGS   SFF++ E  +WK +T N   +KIKYYKGLGTST +E K+YFSD+K H+I+F    D+D  +I+MAFSKK I+ RK WL  ++        LG S +   EKD   +SY DF+NKEL+ +S  D ERSIP+++DG+KPGQRKV++ C KRN + E KVAQL G + E SAYHH GE+SL  TIIN+AQ FVGSNNIN L P GQFG+R  GGKD ++ RYIFT+++  TR IFN  DD +L YL ++ QKIEP++Y+P+IP +LVNG +GIGTG+ + IPNYN ++I+ N+   I+      M PW+K FKG IES     Y   G I  I  + +E+TELP++ WTQ YKE  +E +L  +++   +I DY +  +   + F ++M   KL++AE ++GL + FKL++T++T++M LFD    L++Y   L+I++EF + RL  YE RK+YL   LE E   +SN+ +FIL   +  L+V  KK+K L+EEL RKG+D    + K K  +  E+      D+  D+E    G+    DYDYLL M ++SLT E+ ++LL +  EK +EL +LR  + E +W  D+++  E ++
Sbjct:    2 AKNKTIEERYQKKSQIEHILLRPDTYIGSVEMHTQLLWVWNKEKNRMVQKNITYVPGLYKIFDEIIVNAADVKAREKEKSENPMTCIKIEINKENKRISVYNDGEGIPVDIHKEMNIYVPHMIFGELLTSDNYDDAEDRITGGRNGFGAKLTNIFSKEFIVQCGDSSRKKEFKMTWSDNMSKFSEPHIKNYN-GKDYVKVTFKPDLNKFGMTEMDDDIESLLFKRVYDLAGTC-SVRVYLNGQRLAVKDFKSYVDLYLKDNSNDNKNNKGQNDNNNNNNNNNDENANQNNDNLDVSLSNEPADGTPTKNNNNNNNNNDEDEIVKIHEKQ-HRWEIVVSKSDGSQFQQVSFVNSICTTKGGSHVNYIVEQLLSSLSKKANAKNKGGMEIKSGHIRNHLWVFVNCLIVNPTFDSQTKETLTTKPVKFGSKCILSDKTINNVLKSPILSNILLWAQAKAQVELKKKMKAGSSKARERIIGIPKLEDANDAGSKYSQECTLILTEGDSAKTSCLAGLSIVGRDKYGVFPLKGKLLNVRDASFKQLMDNKEIQNIFRIMGLDITDK--NKDDIKGLRYGSLMIMTDQDYDGSHIKGLLINMIHKFWPSLLKHKGFLSEFVTPIVKVQKGSQEYSFFTIAEYEQWKENT-NLLGWKIKYYKGLGTSTDREFKQYFSDIKNHKIMFLWTGDRDGDSIDMAFSKKRIEDRKLWLQNFI--------LG-SYVDHKEKD---LSYYDFVNKELIYYSRYDTERSIPNIMDGWKPGQRKVLYGCFKRNLRNECKVAQLVGYIAEHSAYHHHGESSLQQTIINMAQTFVGSNNINFLEPCGQFGSRKEGGKDASAARYIFTKLASSTRSIFNEYDDPILKYLNEEGQKIEPQYYIPVIPTILVNGCEGIGTGYSSFIPNYNYKDIIDNIKRYINKEPLIPMVPWYKDFKGRIESNGKTGYETIGIINKIDNDTLEITELPIKKWTQDYKE-FLEELL--TDEKHQLILDYIDNSSHEDICFTIKMDPAKLQKAE-EEGLEKVFKLKSTLTTTNMTLFDPNLKLQRYSTELDILKEFCYQRLKAYENRKSYLISKLEKEKRIISNKTKFILAIVNNELIVNKKKKKVLVEELYRKGYDPYKDINKIKKEEIFEQELLDAADNPEDNEEIIAGITVK-DYDYLLSMPIFSLTLEKVEDLLTQLKEKERELEILRNITVETMWLKDIEKVEEAIE 1199          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1215154270|gb|ASK50299.1| (DNA topoisomerase 2 [Eyprepocnemis plorans])

HSP 1 Score: 1768.05 bits (4578), Expect = 0.000e+0
Identity = 836/1245 (67.15%), Postives = 1013/1245 (81.37%), Query Frame = 0

Query:   35 ANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK----MEEDSDS-DSEGGLEKDLD--YDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEA-------LPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKK 1265
            A G+A  P A     K ++VE+IYQKKSQLEHILLRPDTYIGSV PVTE MW+ D E + +V +EITYVPGLYKIFDEILVNAADNKQRD  MD IKIDI+ E N + V+NNG+GIPV  HKDEKM+VPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FSTKFTVETAS+E ++ FKQ W NNMSK  + K+KDF+ G DYT++TF+PDL KF ME+LD D V+L+SRRAYD+AAS++GVKV LNGKR+ VK FKDYV+ ++K  EDETG+P+K +YE   +RWEVA+ +SD GFQQ+SFVNSIATTKGGRHVD+VS MI   + E LKKKNK G+ IK  Q++NH+WLF+NCLIVNPTFDSQTKENMTL    FGSKC  + KF   + KSG+VES+L W+K KAD  +    + KK ++L GI KLEDAN AGT++S+DCTLILTEGDSAK+LAV+GLGV+GRDK+GV+PLRGKLLNVREA+HKQI++N EIN +++I+GL YKKKY +V+DLK+LRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLL+LPFLEEFITPIVKA+KG+   SF+SLPE  EWK+  DNWHTYKIKYYKGLGTSTSKEAKEYF DMKRH+I F +    DD  INMAFSKK I+QRKEWLT+WM++ KRRKE+GL E YLYEKDT +V+Y+DF+NKELVLFSN DNERSIP +VDGFKPGQRKV+FTCLKRNDKREVKVAQLAGSV E SAYHHGE SLM TIINLAQN+VGSNNIN+L PIGQFGTRL GGKD ASPRYIFT++SPL R IF+ +DDA+L + TDDNQ+IEP WY+P+IPMVLVNGA+GIGTGWMTKIPNYNPREI+ NL  MIDG  P+ M PW+K++KG IE   + +Y+V GEIA + E+K+E+TELPV  WTQ+YKE V+EPML G+EK+P  I  ++EY+TD TV+FVV+M   KL++AE  +GLH+FFKL+T++S +SM  FD   CL++Y++V+EI++EFF+LRLN+Y KRK YLEGML+AESL+LSNQARFILEKCDG LVVENKK+K +IEEL+ +G+D DPV++WK  Q     +EE +++ DS+    +D+D  YDYLLGM MW+LT+ERKDELL ++DEK QE  +L+G S  DLW+ DL   +E+LD+VEAKE EDE   +  +  KG K     K+ A       LPSP G RV P +  +   KA K  A K  KG++  KK
Sbjct:    2 AGGDA--PPAPKKNVKNMTVEKIYQKKSQLEHILLRPDTYIGSVEPVTELMWVYDPEQEMMVQREITYVPGLYKIFDEILVNAADNKQRDPKMDCIKIDIDAENNTVSVWNNGKGIPVVEHKDEKMYVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTKFTVETASKEYKRAFKQTWANNMSKTSDPKIKDFA-GEDYTKVTFSPDLTKFKMEALDKDIVSLMSRRAYDVAASTRGVKVMLNGKRLPVKTFKDYVELYIKNKEDETGNPLKIVYEQVNDRWEVALTISDKGFQQVSFVNSIATTKGGRHVDHVSDMIVKQLIETLKKKNKNGIQIKAFQVKNHMWLFVNCLIVNPTFDSQTKENMTLQAKNFGSKCPLSEKFINNVTKSGIVESILMWAKAKADSLLTKACNKKKQSRLTGIPKLEDANHAGTQHSLDCTLILTEGDSAKSLAVSGLGVIGRDKYGVFPLRGKLLNVREASHKQIMDNAEINNIIRIVGLQYKKKYESVNDLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLQLPFLEEFITPIVKASKGNQEFSFYSLPEFEEWKASKDNWHTYKIKYYKGLGTSTSKEAKEYFMDMKRHRIKFNYGGPNDDQCINMAFSKKCIEQRKEWLTSWMQDSKRRKEMGLPEQYLYEKDTHSVNYADFVNKELVLFSNCDNERSIPCMVDGFKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEHSAYHHGEQSLMSTIINLAQNYVGSNNINVLQPIGQFGTRLNGGKDSASPRYIFTKLSPLARFIFHKHDDALLKHNTDDNQRIEPVWYIPVIPMVLVNGANGIGTGWMTKIPNYNPREIIDNLRRMIDGEAPKQMNPWYKNYKGVIEPFGDSKYIVYGEIAVLGEDKVEITELPVGVWTQTYKENVLEPMLGGTEKVPQQIVQFQEYNTDTTVRFVVKMDPAKLQKAE-QEGLHKFFKLETSMSLNSMCAFDDMQCLKRYDSVIEILKEFFNLRLNYYGKRKLYLEGMLQAESLRLSNQARFILEKCDGVLVVENKKKKVIIEELKTRGYDPDPVRQWKLKQDKEALLEESNNADDSQDEATEDIDKLYDYLLGMTMWTLTKERKDELLSKRDEKLQEYEILKGKSPADLWKEDLDALLEELDKVEAKEREDEASGVVGKPVKGKKAAGSTKLAAISQKPVTLPSPGGRRVAPKLDPDRIKKAEKTTAVKENKGKRQVKK 1242          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1339043062|ref|XP_023709584.1| (DNA topoisomerase 2 isoform X2 [Cryptotermes secundus] >gi|1330910156|gb|PNF31624.1| DNA topoisomerase 2 [Cryptotermes secundus])

HSP 1 Score: 1756.88 bits (4549), Expect = 0.000e+0
Identity = 861/1351 (63.73%), Postives = 1063/1351 (78.68%), Query Frame = 0

Query:   36 NGEAKKPKAGGGGA------KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGS-EKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEED--SDSDSEG-----GLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILS-KESQKGAKGKKKLKIEAL------PSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS 1365
            NG+  +  +GG  A      K+L+VE+IYQKKSQLEHILLRPDTYIGSV PV E MW+ DEE   +V KEI+YVPGLYKIFDEILVNAADNKQRD  MD IKI+IN EKN I ++NNG+GIPV  HKDEKMFVPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FST F VETA++E ++ FKQVW +NMSK  E ++KD  +G D+T+ITF+PDL KF ME+LD D VAL+S+RA+D+AAS++GVKV+LNGKR+ VK+FKDYV+ +LKG ED+ G+P+K +YE    RWEVAV++S+ GFQQ+SFVNSIATTKGGRHVD++++ I   + E LKKKNKGG+ IKPHQ++ HLW+F+NCLIVNPTFDSQTKE MTL    FGSKC+ + KF   + KS +VE+VL W+K KA+++++ + SSKK NKLKG+ KLEDANDAG++NS+DCTLILTEGDSAKTL V+GLGVVGRDK+GV+PLRGKLLNVREATHKQI EN+EI  ++KI+GL YK KY ++DDLK+LRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLL+LPFLEEFITPIVKATKGS   SF+SLPE  EWK+ T+NWHTYKIKYYKGLGTSTSKEAKEYFSDM+RH+I FK+    DDH INMAFSKK  +QRKEWLT+WME+ KRR+ELGL E YLYEKDTK+V+YSDF+NKELVLFSN DNERSIPS+VDG KPGQRKV+FTCLKR DKRE+KVAQLAGSV E S+YHHGE SLM TI+NLAQNFVGSNNINLL PIGQFGTRL+GGKD ASPRYIFT +SPLT  IF+ +D  +L +  DD+QKIEP WY+PIIPMVLVNGADGIGTGWMTKIPNYNPREIV+NL  M+D  EPE M PW+K+F G +E + ++RY++ GEIA I E+K+E+TELP+ +WTQ+YKE V+EP+L+G+ EK P  I DYKEY+TD TVKFVV M+ +K R AE + GLH+FFKLQTT+S SSM +FD   C+RK+E+V  IM+EFF +RL +YEKRK Y EGML+AES +LSNQARFI+EKCDG+LV+ENKK K +IEEL+R G+D DPV  WK  Q   E    + D+EG     G +    Y+YLL MA+++LT+E+K++LLR++D+K  +L++L+  S   LW+ DL  F+E+LD+VEAKE EDE  + S K ++ G K     K E L      PSP G RV+P I  +LK K  K   +K  KG++  K+ L   E  KDEFD M  ++   ++L+++LG S  D   K         +++P+    ++  K         E+D+ VE  D + S  D+  S+  P++ S
Sbjct:    7 NGQVPEMMSGGDVAPKKPPNKKLTVEKIYQKKSQLEHILLRPDTYIGSVEPVVETMWLYDEEQDAMVQKEISYVPGLYKIFDEILVNAADNKQRDPKMDCIKIEINPEKNIISIWNNGQGIPVVEHKDEKMFVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTMFIVETAAKEYKRSFKQVWGSNMSKTSEPRIKD-HTGEDFTKITFSPDLSKFRMETLDKDIVALMSKRAFDVAASTRGVKVYLNGKRLPVKSFKDYVELYLKGKEDDQGNPLKCVYENVNPRWEVAVSLSEKGFQQVSFVNSIATTKGGRHVDHIAEHIVKQLIETLKKKNKGGLQIKPHQVKTHLWVFVNCLIVNPTFDSQTKETMTLQAKNFGSKCNLSDKFITNVTKSSIVEAVLTWAKSKAEKDLQKQCSSKKQNKLKGVPKLEDANDAGSRNSLDCTLILTEGDSAKTLVVSGLGVVGRDKYGVFPLRGKLLNVREATHKQIKENQEIGNVIKILGLQYKMKYESIDDLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLKLPFLEEFITPIVKATKGSTELSFYSLPEFEEWKASTNNWHTYKIKYYKGLGTSTSKEAKEYFSDMQRHRIKFKYGGPNDDHCINMAFSKKCAEQRKEWLTSWMEDTKRRRELGLPEQYLYEKDTKSVNYSDFVNKELVLFSNCDNERSIPSLVDGLKPGQRKVLFTCLKRADKREIKVAQLAGSVAEHSSYHHGEQSLMSTIVNLAQNFVGSNNINLLQPIGQFGTRLSGGKDSASPRYIFTLLSPLTSFIFHPHDMPLLKHNYDDSQKIEPVWYIPIIPMVLVNGADGIGTGWMTKIPNYNPREIVKNLKLMLDDQEPEPMVPWWKNFTGVVEPLVDKRYIIYGEIAVIGEDKLEITELPIGTWTQTYKESVLEPLLHGANEKTPPSITDYKEYNTDTTVKFVVTMNPEKRRAAEAE-GLHKFFKLQTTVSLSSMCVFDENCCMRKFESVELIMKEFFKVRLAYYEKRKKYHEGMLQAESRRLSNQARFIIEKCDGTLVIENKKMKVMIEELKRNGYDPDPVAVWKMKQNKAEALMENEDAEGSQLDDGSDVGGGYNYLLAMALYTLTEEKKEDLLRKRDDKLTQLKILQTKSPAALWKEDLDVFLEQLDKVEAKEKEDELGVSSGKTTKVGKKFGGSKKKEMLTAAETKPSPQGRRVIPKIDVKLKEKFEKETLSKENKGKRARKEKLLIEE--KDEFDMMDGNQ---KTLADRLGTSPEDIEKKMNAKRKEVKQRKPRKESSSRSPKKKGKGKNPWETDSEVE--DEELSSEDDGVSRPPPRTTS 1348          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1339043058|ref|XP_023709582.1| (DNA topoisomerase 2 isoform X1 [Cryptotermes secundus] >gi|1339043060|ref|XP_023709583.1| DNA topoisomerase 2 isoform X1 [Cryptotermes secundus])

HSP 1 Score: 1754.96 bits (4544), Expect = 0.000e+0
Identity = 861/1355 (63.54%), Postives = 1063/1355 (78.45%), Query Frame = 0

Query:   36 NGEAKKPKAGGGGA------KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGS-EKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEED------SDSDSEG-----GLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILS-KESQKGAKGKKKLKIEAL------PSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS 1365
            NG+  +  +GG  A      K+L+VE+IYQKKSQLEHILLRPDTYIGSV PV E MW+ DEE   +V KEI+YVPGLYKIFDEILVNAADNKQRD  MD IKI+IN EKN I ++NNG+GIPV  HKDEKMFVPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FST F VETA++E ++ FKQVW +NMSK  E ++KD  +G D+T+ITF+PDL KF ME+LD D VAL+S+RA+D+AAS++GVKV+LNGKR+ VK+FKDYV+ +LKG ED+ G+P+K +YE    RWEVAV++S+ GFQQ+SFVNSIATTKGGRHVD++++ I   + E LKKKNKGG+ IKPHQ++ HLW+F+NCLIVNPTFDSQTKE MTL    FGSKC+ + KF   + KS +VE+VL W+K KA+++++ + SSKK NKLKG+ KLEDANDAG++NS+DCTLILTEGDSAKTL V+GLGVVGRDK+GV+PLRGKLLNVREATHKQI EN+EI  ++KI+GL YK KY ++DDLK+LRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLL+LPFLEEFITPIVKATKGS   SF+SLPE  EWK+ T+NWHTYKIKYYKGLGTSTSKEAKEYFSDM+RH+I FK+    DDH INMAFSKK  +QRKEWLT+WME+ KRR+ELGL E YLYEKDTK+V+YSDF+NKELVLFSN DNERSIPS+VDG KPGQRKV+FTCLKR DKRE+KVAQLAGSV E S+YHHGE SLM TI+NLAQNFVGSNNINLL PIGQFGTRL+GGKD ASPRYIFT +SPLT  IF+ +D  +L +  DD+QKIEP WY+PIIPMVLVNGADGIGTGWMTKIPNYNPREIV+NL  M+D  EPE M PW+K+F G +E + ++RY++ GEIA I E+K+E+TELP+ +WTQ+YKE V+EP+L+G+ EK P  I DYKEY+TD TVKFVV M+ +K R AE + GLH+FFKLQTT+S SSM +FD   C+RK+E+V  IM+EFF +RL +YEKRK Y EGML+AES +LSNQARFI+EKCDG+LV+ENKK K +IEEL+R G+D DPV  WK  Q   E        + D+EG     G +    Y+YLL MA+++LT+E+K++LLR++D+K  +L++L+  S   LW+ DL  F+E+LD+VEAKE EDE  + S K ++ G K     K E L      PSP G RV+P I  +LK K  K   +K  KG++  K+ L   E  KDEFD M  ++   ++L+++LG S  D   K         +++P+    ++  K         E+D+ VE  D + S  D+  S+  P++ S
Sbjct:    7 NGQVPEMMSGGDVAPKKPPNKKLTVEKIYQKKSQLEHILLRPDTYIGSVEPVVETMWLYDEEQDAMVQKEISYVPGLYKIFDEILVNAADNKQRDPKMDCIKIEINPEKNIISIWNNGQGIPVVEHKDEKMFVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTMFIVETAAKEYKRSFKQVWGSNMSKTSEPRIKD-HTGEDFTKITFSPDLSKFRMETLDKDIVALMSKRAFDVAASTRGVKVYLNGKRLPVKSFKDYVELYLKGKEDDQGNPLKCVYENVNPRWEVAVSLSEKGFQQVSFVNSIATTKGGRHVDHIAEHIVKQLIETLKKKNKGGLQIKPHQVKTHLWVFVNCLIVNPTFDSQTKETMTLQAKNFGSKCNLSDKFITNVTKSSIVEAVLTWAKSKAEKDLQKQCSSKKQNKLKGVPKLEDANDAGSRNSLDCTLILTEGDSAKTLVVSGLGVVGRDKYGVFPLRGKLLNVREATHKQIKENQEIGNVIKILGLQYKMKYESIDDLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLKLPFLEEFITPIVKATKGSTELSFYSLPEFEEWKASTNNWHTYKIKYYKGLGTSTSKEAKEYFSDMQRHRIKFKYGGPNDDHCINMAFSKKCAEQRKEWLTSWMEDTKRRRELGLPEQYLYEKDTKSVNYSDFVNKELVLFSNCDNERSIPSLVDGLKPGQRKVLFTCLKRADKREIKVAQLAGSVAEHSSYHHGEQSLMSTIVNLAQNFVGSNNINLLQPIGQFGTRLSGGKDSASPRYIFTLLSPLTSFIFHPHDMPLLKHNYDDSQKIEPVWYIPIIPMVLVNGADGIGTGWMTKIPNYNPREIVKNLKLMLDDQEPEPMVPWWKNFTGVVEPLVDKRYIIYGEIAVIGEDKLEITELPIGTWTQTYKESVLEPLLHGANEKTPPSITDYKEYNTDTTVKFVVTMNPEKRRAAEAE-GLHKFFKLQTTVSLSSMCVFDENCCMRKFESVELIMKEFFKVRLAYYEKRKKYHEGMLQAESRRLSNQARFIIEKCDGTLVIENKKMKVMIEELKRNGYDPDPVAVWKMKQNKAEALENGMMENEDAEGSQLDDGSDVGGGYNYLLAMALYTLTEEKKEDLLRKRDDKLTQLKILQTKSPAALWKEDLDVFLEQLDKVEAKEKEDELGVSSGKTTKVGKKFGGSKKKEMLTAAETKPSPQGRRVIPKIDVKLKEKFEKETLSKENKGKRARKEKLLIEE--KDEFDMMDGNQ---KTLADRLGTSPEDIEKKMNAKRKEVKQRKPRKESSSRSPKKKGKGKNPWETDSEVE--DEELSSEDDGVSRPPPRTTS 1352          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|321457974|gb|EFX69050.1| (hypothetical protein DAPPUDRAFT_329527 [Daphnia pulex])

HSP 1 Score: 1746.48 bits (4522), Expect = 0.000e+0
Identity = 855/1279 (66.85%), Postives = 1035/1279 (80.92%), Query Frame = 0

Query:   35 ANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGS----LSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK--------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKG---AKGKKKLKI-EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVS 1297
             NG+A K     G  KRLSVERIYQKKSQLEHILLRPDTYIGSV PV + MW+  EE+  ++ +EITYVPGLYKIFDEILVNAADNK RD  MDTIK+DI+ ++N I ++NNG+GIPV  HK+EKM+VP+LIFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FST F +ETAS++ RK+FKQ W +NM+KA   K+ +++  +D+T+ITF+PDL KF M+SLD DTVALLSRRA+D+AAS++GVKVFLNGKR+ +KNFKDYVD  LKG ED+ G+ +K +YEA  ERWE+AVA SD GFQQ+SF NSIATTKGGRHVDYV++++   + E LKKKNK G+ +KPHQI+NHLW+F+NCLIVNPTFDSQTKENMTL    FGS C  + KFF  L KSGV+E V+ W+KFKA  E+    S KK  KLKG+ KLEDAN+AGTK+SIDCTLILTEGDSAK+LAV+GLGVVGRDK+GV+PL+GK+LNVREATHKQI+EN EIN ++KI+GL YKKKY +V+DLKSLRYG+LMIMTDQDQDGSHIKGLLINF+HHNWPSLLRLPFLEEFITPIVKATKG+     S SF+SLPE  EWK++T+NW TY+IKYYKGLGTSTS+EAK YFSDM+RH+ILF H+ DQDD+AI MAFSKK ++QRKEWLT+WMEECKRR+ELGL E+YLYEKDTK VSY DF+NKELVLFSN+DNERSIPS++DGFKPGQRKV+FTC+KRNDK+E+KVAQLAGSV E SAYHHGEASLMGTII LAQ+FVGSNNINLLLP GQFGTRL GGKD ASPRYIFT MS LTR+IF+ +DD +L +  DDNQ+IEPE+Y PIIPM+LVNG DGIGTGWMTKI NYNPRE+V N+  M+DG EP  M PWFK ++G IE I+  RY ++GE+A +S  ++E+TELP+R WTQ+YKE VME +L+GSEKIP +I DYKEYHTD TV+F+V MS +K++QAE + GLH+ FKLQTT S +SMVL+D+ GCL+ Y+ V +I++EFF +RL +Y KRK+YL G+L+AES +LSNQARFI EKCD  L+VENKK K L+EEL+R+ +D DPV+ WK  QK         EE++++++E G  K  DYDYLL M M SLT E+K+ELL+++D K +E  +L   S  DLWR DL  FM  L EVE  + E E +  +K+  K    AKG+KK  I E  PSP G R+VP+I  EL+ K  K   AK +K EK  KK   EVE  +D FD   DD   N+SL EKLGVS
Sbjct:   25 TNGDAGKK---NGANKRLSVERIYQKKSQLEHILLRPDTYIGSVEPVKDVMWVY-EEDAGMIQREITYVPGLYKIFDEILVNAADNKVRDPKMDTIKVDIDPKENVITIYNNGKGIPVVEHKEEKMYVPSLIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTSFELETASKDERKKFKQTWTDNMTKAGTPKIVNYNE-ADFTQITFSPDLAKFKMDSLDKDTVALLSRRAFDVAASTRGVKVFLNGKRLPIKNFKDYVDLCLKGKEDDDGAQLKCVYEAVSERWEIAVAPSDKGFQQVSFANSIATTKGGRHVDYVTEIVSKYLQEFLKKKNKSGINVKPHQIKNHLWIFVNCLIVNPTFDSQTKENMTLQAKSFGSTCKPSEKFFNALNKSGVIEGVMTWAKFKAQTELNKACSGKKQTKLKGVPKLEDANNAGTKHSIDCTLILTEGDSAKSLAVSGLGVVGRDKYGVFPLKGKVLNVREATHKQILENAEINNLIKILGLQYKKKYESVEDLKSLRYGRLMIMTDQDQDGSHIKGLLINFLHHNWPSLLRLPFLEEFITPIVKATKGTGASAKSLSFYSLPEFEEWKTNTENWPTYRIKYYKGLGTSTSQEAKGYFSDMQRHRILFHHDGDQDDYAILMAFSKKHVEQRKEWLTSWMEECKRRRELGLPELYLYEKDTKRVSYRDFVNKELVLFSNMDNERSIPSLMDGFKPGQRKVLFTCIKRNDKKEIKVAQLAGSVAEHSAYHHGEASLMGTIIGLAQDFVGSNNINLLLPNGQFGTRLQGGKDAASPRYIFTLMSKLTRIIFHPHDDPLLKHQMDDNQRIEPEYYAPIIPMILVNGTDGIGTGWMTKILNYNPREVVANVKRMLDGEEPIPMDPWFKGYRGTIERIDRHRYAISGELAVLSGTEMEITELPLRVWTQNYKESVMETLLHGSEKIPPLITDYKEYHTDTTVRFIVTMSEEKMQQAEAE-GLHKAFKLQTTHSLTSMVLYDSNGCLKMYDTVEDILKEFFTVRLGYYGKRKDYLSGVLQAESQRLSNQARFICEKCDNVLIVENKKAKVLVEELRRRNYDPDPVRIWKKNQKNNVNAENEEEEEANAENEEGAVKIGDYDYLLDMPMRSLTYEKKEELLKKRDVKMKEYDILLKKSPADLWREDLDHFMVVLKEVE--DAEQEELNENKKPSKAAPLAKGRKKALIAEVAPSPKGRRIVPVIDNELRKKIEKLTIAKEKKKEKALKKESPEVE--RDSFDIALDDS--NKSLGEKLGVS 1291          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1325310473|ref|XP_023336845.1| (LOW QUALITY PROTEIN: DNA topoisomerase 2-alpha-like [Eurytemora affinis])

HSP 1 Score: 1739.16 bits (4503), Expect = 0.000e+0
Identity = 880/1302 (67.59%), Postives = 1049/1302 (80.57%), Query Frame = 0

Query:   39 AKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRN-DKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ--------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRR--KDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPW 1329
            A KPK    G KRLSVERIYQKKSQLEHILLRPDTYIGSV PVTE MW+ ++EN  +VN+E+TYVPG YKI+DEILVNAADNKQRD  MDTIKIDI  E+  I + NNG+GIPV MHK+E MFVPT+IFGHLLTSSNFNDEEEKVTGGRNG+GAKLCNVFSTKFTVETA ++  K FKQ W +NMSKA E K+KDF+ G +YT+IT++PDL KFNME +D DT AL SRRA+DIAAS+KGVKVFLNGKR+ VK+FKDYVD ++KG EDE+G+ +K I+EA G RWEVAV VSD GFQQMSFVNSIATTKGGRHVD+V+  +   I + +KK NK G+ IK  Q++NHLWLF+NCLIVNPTFDSQTKE MTL +  FGSKC  + KF   LQKSG++ESV+AWSKFKAD ++ +K   KKT+KL+GI KLEDAN+AGTKNS  CTLILTEGDSAKTLAVAGLGV+GRD +GVYPL+GKLLNVREATHKQI+ENKEIN++VKIIGL YKKKY T++D+KSLRYGK++I+   + D   IKGL+INFIHHNWP LL+LPFLEEFITPIVK +      SF+        K   D W   +  ++ GLGTSTSKEAKEYFSDM RH+I FK+   QDDH+I +AFSKK I+ RKEWLTTWMEE KRRKELGL E+YLYEKDTK V+Y+DF+NKELVLFSN+DNERSIPS+VDGFKPGQRKV+FTCLKRN  K+E+KVAQLAGSV E+SAYHHGEASLMGTIINLAQN+VGSNNINLL PIGQFGTRL GGKD ASPRYIFTQ+SPL R IFN  DDA+LNY  DDNQKIEPEWY+PI+PMVLVNGADGIGTGWMTKIPNYNPREIV+NL  MIDG EP+ M PWFK FKG IE I++QRY V+GEI+++S+ K+E+TELP+R+WT SYKE ++E +LNG+EK+PA+I D+K+Y+TD TVKF++ M  DKLR AE +KGLH FFKLQT+IS +SMVLFD  GCLR++++V EI+REFF LRL FY KRK YLEGML AE+ KLSNQARFILEKCD +L +ENKK+K +IEELQR+G+DSDP+K WK +Q          +E++     GG     DYDYLLGM +W LTQE+KDELL++  +  +   + +L  TSKE+LW+ DL  F+ KLDE E  E+ D+    +K  +KG   K  LK+EALPSP+GIRV P IA+ELK K AKA AAK+RK +K DKK++K+ E  KDEFD M +DK++N+SLSE++G    +K  K+      T     +SSPK KGK PW
Sbjct:   16 APKPK----GDKRLSVERIYQKKSQLEHILLRPDTYIGSVQPVTEPMWVYEDEN--MVNREVTYVPGFYKIYDEILVNAADNKQRDPKMDTIKIDIKPEEGVISIMNNGKGIPVVMHKEENMFVPTMIFGHLLTSSNFNDEEEKVTGGRNGFGAKLCNVFSTKFTVETADKKAGKGFKQTWASNMSKAGEPKIKDFN-GEEYTKITYSPDLKKFNMEKIDDDTFALFSRRAFDIAASTKGVKVFLNGKRLPVKSFKDYVDQYIKGKEDESGNQIKCIHEACGPRWEVAVCVSDKGFQQMSFVNSIATTKGGRHVDHVADAVIKAIIDKIKKSNKTGINIKNFQVKNHLWLFVNCLIVNPTFDSQTKETMTLGVKDFGSKCVLSDKFQTSLQKSGIIESVVAWSKFKADMKLSSKAGGKKTSKLRGIAKLEDANEAGTKNSGGCTLILTEGDSAKTLAVAGLGVIGRDYYGVYPLKGKLLNVREATHKQILENKEINDLVKIIGLAYKKKYETMEDIKSLRYGKVLILLKLEFDTPQIKGLVINFIHHNWPGLLKLPFLEEFITPIVKVSHYRSLMSFYH-------KVGYDFW--VQFIHFIGLGTSTSKEAKEYFSDMVRHRIKFKYNGSQDDHSITLAFSKKEIEGRKEWLTTWMEEGKRRKELGLPEVYLYEKDTKAVNYTDFVNKELVLFSNMDNERSIPSLVDGFKPGQRKVVFTCLKRNLTKKEIKVAQLAGSVAEISAYHHGEASLMGTIINLAQNYVGSNNINLLQPIGQFGTRLQGGKDSASPRYIFTQLSPLARHIFNEADDALLNYQLDDNQKIEPEWYIPILPMVLVNGADGIGTGWMTKIPNYNPREIVKNLKLMIDGKEPKPMIPWFKGFKGTIEEIDSQRYAVSGEISTLSDTKVEITELPIRTWTNSYKE-LLEGLLNGTEKLPAVISDFKDYNTDRTVKFIITMDKDKLRNAEREKGLHAFFKLQTSISNTSMVLFDHLGCLRRFDSVEEILREFFTLRLEFYGKRKRYLEGMLGAEAAKLSNQARFILEKCDNTLTIENKKKKVMIEELQRRGYDSDPIKAWKKSQDNMDEEAAGEDEEAAESPAGG----PDYDYLLGMKLWCLTQEKKDELLKKLWRCXRINXIYILXATSKEELWKVDLDAFLAKLDETEDAELADD-AGAAKLGKKGPGKKGALKLEALPSPMGIRVEPRIADELKVKVAKAVAAKARKADKGDKKSVKK-EDDKDEFDLMTEDKELNKSLSERIGAVKKEKPLKQ------TKLNFEKSSPKKKGKNPW 1288          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1316162989|ref|XP_023226762.1| (DNA topoisomerase 2-alpha-like isoform X1 [Centruroides sculpturatus] >gi|1316162991|ref|XP_023226763.1| DNA topoisomerase 2-alpha-like isoform X2 [Centruroides sculpturatus])

HSP 1 Score: 1736.08 bits (4495), Expect = 0.000e+0
Identity = 843/1317 (64.01%), Postives = 1043/1317 (79.20%), Query Frame = 0

Query:   50 KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGGLEKDL---DYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKES---QKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAK----AAAAKSR-KGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKK------PWEDSDATSESDNSVEFDDFDD 1349
            KRLSVERIYQKK+QLEHILLRPDTYIGSV PVT+ MW+ D     ++ +++++VPGLYKIFDEILVNAADNKQRDK+MD IK+DI+ E N+I ++NNG+GIPV  HK EK+FVP+LIFGHLLTSSN+NDEE+KVTGGRNGYGAKLCN+FSTKF VET+S+E +K FKQ W +NMSK  EA++   + G D+T +TF+PDL KF ME LD DTVAL+SRR YD+A SS+GVKV+LNGKR+ ++ FK+YV+ ++K   DE G+P+K +YE+  +RWEVAV +S+ GFQQ+SFVNSIATTKGGRHVDYV++ I S +A+V+KKKNKGGV IKP QI+NH+W+F+NCLI NPTFDSQTKENMTL    FGSKC  + KFF Q+ K G++E +++W +FKA  E+  K S+ K +KL+GI KLEDANDAGTK SIDCTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREATHKQI+EN+EIN ++KI+GL YKKKY ++DDLK+LRYG+LMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVK +KG    SF+SLPE  EWK  T NW+T+KIKYYKGLGTSTSKEAKEYF+DM RH+I FK+E  QDD+AI +AFSKK +DQRKEWLT  MEE K R+ELGL E+YLY K+T  ++++DF+NKEL+LFSN+DNERSIPS+VDG KPGQRKVM+TC KRNDKRE+KVAQLAGSV E SAYHHGE++LMGTIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT +SPL +LIF+  D+ +L +L DDNQ+IEPE+Y+P+IPMVLVNG +GIGTGW TKIPNYNPREIV N+  M+ G EP  M PWFK+FKG IE ++NQRYV+NGE++ +S NK+E+TELPVR+WTQ+YKEQV+E ML+GS+K+P++I DYKEYHTD TV+FVV M+ +KL ++E ++GLH+ FKLQTT+STSSMVLFD KGCLR+Y++  EI++EFF +RL+ YE+RK YLEGML AES KL +QARFILEK +G +++EN+K+  LI  LQ +G++SDPVK WK +Q    D++  +E   E+D    DYDYLLGM +WSLT+ERKDELL+++D+K  EL  LR  +  DLW  DL   M +LD VE KE EDE +  +K++    K  KG+K    E  PSP+G RV+P I  +LK +A K        K R KG+K D + +KE          +C++ +      E LG S      KK+   SST  K  + S   KG K      PW      +ES+NS    D DD
Sbjct:   30 KRLSVERIYQKKTQLEHILLRPDTYIGSVEPVTQSMWVYDGPETGMIFRDVSFVPGLYKIFDEILVNAADNKQRDKSMDCIKVDIDPENNKISIWNNGKGIPVVEHKVEKIFVPSLIFGHLLTSSNYNDEEKKVTGGRNGYGAKLCNIFSTKFVVETSSKEYKKSFKQCWTDNMSKTTEARIIP-NKGEDFTCVTFSPDLSKFGMEVLDKDTVALMSRRVYDVAGSSRGVKVYLNGKRLPIRGFKEYVEQYIKDKNDEQGNPLKVVYESVNDRWEVAVTMSERGFQQVSFVNSIATTKGGRHVDYVTEQIVSKLADVVKKKNKGGVQIKPFQIKNHMWVFVNCLIENPTFDSQTKENMTLQAKNFGSKCPLSDKFFNQVIKCGIIECIMSWVRFKAQTELNKKCSATKHSKLRGIPKLEDANDAGTKGSIDCTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKMLNVREATHKQILENQEINNIIKIMGLQYKKKYESIDDLKTLRYGRLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKISKGKEEMSFYSLPEYEEWKQQTPNWNTWKIKYYKGLGTSTSKEAKEYFADMNRHRIKFKYEGTQDDNAIQLAFSKKLVDQRKEWLTNGMEERKHRRELGLPELYLYGKNTTNITFNDFVNKELILFSNMDNERSIPSLVDGLKPGQRKVMYTCFKRNDKREIKVAQLAGSVAEHSAYHHGESALMGTIINLAQNFVGSNNINLLQPIGQFGTRLQGGKDAASPRYIFTMLSPLAKLIFSQLDEPLLVHLFDDNQRIEPEYYIPVIPMVLVNGCEGIGTGWSTKIPNYNPREIVSNIRRMLKGEEPVKMNPWFKNFKGVIEQLDNQRYVINGEVSILSSNKLEITELPVRTWTQTYKEQVLETMLHGSDKVPSLITDYKEYHTDSTVRFVVTMNEEKLSKSE-EQGLHKVFKLQTTLSTSSMVLFDHKGCLRRYDSPEEILKEFFEVRLSCYERRKAYLEGMLTAESKKLDSQARFILEKIEGKIIIENRKKSELIRTLQERGYESDPVKSWKESQNKNTDNEETNEDDEERDSSGPDYDYLLGMNLWSLTRERKDELLKKRDDKRAELEALREKTPADLWEDDLTNLMVELDRVEEKEREDEIIGGTKKAIKLGKSGKGRKVALEETKPSPMGQRVIPHIDPDLKKRAEKTPLGTGEGKRRGKGKKTDSELIKE----------LCENGE------EGLGNSDKSNKMKKKLKCSSTLLKAVKKSGSPKGNKKGPKRNPWSSESDGNESNNS----DVDD 1324          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1067070039|ref|XP_018018369.1| (PREDICTED: DNA topoisomerase 2-alpha-like isoform X3 [Hyalella azteca])

HSP 1 Score: 1734.15 bits (4490), Expect = 0.000e+0
Identity = 881/1489 (59.17%), Postives = 1109/1489 (74.48%), Query Frame = 0

Query:   43 KAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ-------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVI---LSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPK-----KRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS--------EVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKKPPAAATKKSKKTSQGSDYDD 1508
            +A     K+ +VE+ YQKK+QLEHI+LRPDTYIGSV  VTE+MW+ D  +++ V +EITYVPGLYKIFDEILVNAADNKQRD  MDTIKI+I+ E N I V+NNG GIPV MHKDEKMFVPT+IFGHLLTSSN+ND+E+KVTGGRNG+GAKLCN+FSTKFTVETA++E +K FKQ W  NMSKA + K+KDF+ G +YT+ITF+PDL KF M+ LD DTV LLSRRAYD+AASS GVKV+LNGKR+ VK+FKDYV+ ++K   D+ G+P+K +YE  G RWEVAV +S+ GFQ MSFVNSIATTKGGRHVDY++ MI +++ E L KKNK GV IK  QI+NHLW+F+N LI NPTFDSQTKENMTL    FGSKC+ + KF   + KSG+VE+V+AW+KFK   +++ K + KKT+KLKGI KLEDANDAGT++S +CTLILTEGDSAKTLAVAGLGVVGRDK+GV+PLRGK+LN REAT KQ++EN EIN +++I+GL YKKKY TVDDLK+LRYGKLMIMTDQDQDGSHIKGL+INFIHHNWPSLLRL FLEEFITPIVKATKG    SF+SLPE  EWKS+ DNWHTYKIKYYKGLGTSTSKEAKEYFSDM RH+I FK+E DQDD +I MAFSKKA DQRKEWLT WM ECKRR+ELGL EIYLYEK+T +V+YSDF+NKELVLFSN DNERSIPS+VDGFKPGQRKVMFTC+KRNDKREVKVAQLAGS+ E +AYHHGE SLM TIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT++SPL R + + +DD +L YL +DNQKIEPEWYMPIIP+VLVNGA+GIGTGW TKIPNYNPREIV N+  MI G EP+DM PWFK+F+G +E ++  RY+ +GE+A + +N+ E++ELP+R+WTQSYKE V+E +L G++KIP II DY+EY+TD TV+FV+ +  +++R  E + G H+ FKLQ+++S ++MVLFDA G L++Y +V+++++EF+ LRL FYEKRK Y+ GML+AE+ KLSNQARFI EKC+G LVVENKKRK +I EL  KG++SDPVK WK +Q           +     E   +   D+DYLLGM MWSLT+ERK++LL+++ EK  EL  L+  S+ DLW  DL  F+E+LD+VEAKE E+ERV    L+ + +K    KKK  +E +PSP+G R+ P I  E++ KA K+AAA + K  +  K++  + +    +        +  +SL+ +LG S +    KKR +   T K     K+ + +   KGK PW  SD+ ++  NS       DS       ++ P+S +          + Y        +    +  +S+D  D    +R   P    F LD ++++      P AP+  +P++ +         S++P  KK    S     + D+D +    + K KKP A        AA+TK++  T+ GS+  D
Sbjct:   29 QAAEPAGKKRTVEKTYQKKTQLEHIILRPDTYIGSVEKVTEQMWVFDTADEKFVQREITYVPGLYKIFDEILVNAADNKQRDPKMDTIKIEIDAENNVISVYNNGHGIPVVMHKDEKMFVPTMIFGHLLTSSNYNDDEKKVTGGRNGFGAKLCNIFSTKFTVETANKEYKKNFKQTWAVNMSKASDPKIKDFT-GEEYTKITFSPDLAKFGMDCLDRDTVDLLSRRAYDVAASSTGVKVYLNGKRVPVKSFKDYVELYIKDRTDDAGAPLKVVYEKPGPRWEVAVTISEKGFQHMSFVNSIATTKGGRHVDYITNMIVNSLTETLSKKNKSGVQIKNFQIKNHLWVFVNALIENPTFDSQTKENMTLQQKSFGSKCTLSDKFVAAVVKSGIVETVMAWAKFKQQAQLEKKCAGKKTSKLKGIPKLEDANDAGTRSSSECTLILTEGDSAKTLAVAGLGVVGRDKYGVFPLRGKVLNTREATVKQMMENAEINHIIQIVGLQYKKKYETVDDLKTLRYGKLMIMTDQDQDGSHIKGLIINFIHHNWPSLLRLNFLEEFITPIVKATKGKEEISFYSLPEYEEWKSNKDNWHTYKIKYYKGLGTSTSKEAKEYFSDMVRHRIKFKYEGDQDDRSIQMAFSKKAADQRKEWLTAWMLECKRRRELGLPEIYLYEKNTHSVNYSDFVNKELVLFSNADNERSIPSLVDGFKPGQRKVMFTCIKRNDKREVKVAQLAGSIAEHTAYHHGEVSLMSTIINLAQNFVGSNNINLLEPIGQFGTRLMGGKDAASPRYIFTKLSPLARALIHPHDDPLLKYLQEDNQKIEPEWYMPIIPLVLVNGAEGIGTGWSTKIPNYNPREIVDNIRKMIRGEEPKDMLPWFKNFRGTVEQLDATRYLCSGEVARLEDNQYEISELPIRTWTQSYKENVLE-ILQGNDKIPPIISDYREYNTDTTVRFVLNVLDNQVRHVEAE-GPHKIFKLQSSMSINTMVLFDANGVLKRYNSVMDVLQEFYELRLKFYEKRKLYMVGMLKAEANKLSNQARFIKEKCEGKLVVENKKRKDMINELITKGYESDPVKAWKKSQSREAAVEDEAAEESDAEEEQTDDAADFDYLLGMPMWSLTKERKEDLLKKEQEKKHELETLQAKSESDLWNHDLDVFIEELDKVEAKEREEERVAKENLASKDKKAGGKKKKALLEVMPSPMGERIQPRIDAEMRKKAEKSAAAGAAKAGRPKKRDNPDDDHDDLD---DLVSANTGKSLASRLGTSPD--LVKKRKAGRPTKKNGDGLKQTKINFPKKGKNPWS-SDSDADGGNS-------DSDLSTTNIETVPRSRAVEGKRAAAAKAKYNFSDDDEDDDDDIESLHSNDGVD----ERHVGPEKLSF-LDQTEDN----AAPDAPSPPKPAAKK---------SNAPKPKKANAHSDEDIVISDTDSEFGDFQQKKKKPSA--------AASTKQAASTADGSNASD 1475          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1067070035|ref|XP_018018366.1| (PREDICTED: DNA topoisomerase 2-alpha-like isoform X1 [Hyalella azteca])

HSP 1 Score: 1731.84 bits (4484), Expect = 0.000e+0
Identity = 880/1496 (58.82%), Postives = 1107/1496 (74.00%), Query Frame = 0

Query:   43 KAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ-------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLK----------IEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPK-----KRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS--------EVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKKPPAAATKKSKKTSQGSDYDD 1508
            +A     K+ +VE+ YQKK+QLEHI+LRPDTYIGSV  VTE+MW+ D  +++ V +EITYVPGLYKIFDEILVNAADNKQRD  MDTIKI+I+ E N I V+NNG GIPV MHKDEKMFVPT+IFGHLLTSSN+ND+E+KVTGGRNG+GAKLCN+FSTKFTVETA++E +K FKQ W  NMSKA + K+KDF+ G +YT+ITF+PDL KF M+ LD DTV LLSRRAYD+AASS GVKV+LNGKR+ VK+FKDYV+ ++K   D+ G+P+K +YE  G RWEVAV +S+ GFQ MSFVNSIATTKGGRHVDY++ MI +++ E L KKNK GV IK  QI+NHLW+F+N LI NPTFDSQTKENMTL    FGSKC+ + KF   + KSG+VE+V+AW+KFK   +++ K + KKT+KLKGI KLEDANDAGT++S +CTLILTEGDSAKTLAVAGLGVVGRDK+GV+PLRGK+LN REAT KQ++EN EIN +++I+GL YKKKY TVDDLK+LRYGKLMIMTDQDQDGSHIKGL+INFIHHNWPSLLRL FLEEFITPIVKATKG    SF+SLPE  EWKS+ DNWHTYKIKYYKGLGTSTSKEAKEYFSDM RH+I FK+E DQDD +I MAFSKKA DQRKEWLT WM ECKRR+ELGL EIYLYEK+T +V+YSDF+NKELVLFSN DNERSIPS+VDGFKPGQRKVMFTC+KRNDKREVKVAQLAGS+ E +AYHHGE SLM TIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT++SPL R + + +DD +L YL +DNQKIEPEWYMPIIP+VLVNGA+GIGTGW TKIPNYNPREIV N+  MI G EP+DM PWFK+F+G +E ++  RY+ +GE+A + +N+ E++ELP+R+WTQSYKE V+E +L G++KIP II DY+EY+TD TV+FV+ +  +++R  E + G H+ FKLQ+++S ++MVLFDA G L++Y +V+++++EF+ LRL FYEKRK Y+ GML+AE+ KLSNQARFI EKC+G LVVENKKRK +I EL  KG++SDPVK WK +Q           +     E   +   D+DYLLGM MWSLT+ERK++LL+++ EK  EL  L+  S+ DLW  DL  F+E+LD+VEAKE E+ERV  +KE+      K   K          +E +PSP+G R+ P I  E++ KA K+AAA + K  +  K++  + +    +        +  +SL+ +LG S +    KKR +   T K     K+ + +   KGK PW  SD+ ++  NS       DS       ++ P+S +          + Y        +    +  +S+D  D    +R   P    F LD ++++      P AP+  +P++ +         S++P  KK    S     + D+D +    + K KKP A        AA+TK++  T+ GS+  D
Sbjct:   29 QAAEPAGKKRTVEKTYQKKTQLEHIILRPDTYIGSVEKVTEQMWVFDTADEKFVQREITYVPGLYKIFDEILVNAADNKQRDPKMDTIKIEIDAENNVISVYNNGHGIPVVMHKDEKMFVPTMIFGHLLTSSNYNDDEKKVTGGRNGFGAKLCNIFSTKFTVETANKEYKKNFKQTWAVNMSKASDPKIKDFT-GEEYTKITFSPDLAKFGMDCLDRDTVDLLSRRAYDVAASSTGVKVYLNGKRVPVKSFKDYVELYIKDRTDDAGAPLKVVYEKPGPRWEVAVTISEKGFQHMSFVNSIATTKGGRHVDYITNMIVNSLTETLSKKNKSGVQIKNFQIKNHLWVFVNALIENPTFDSQTKENMTLQQKSFGSKCTLSDKFVAAVVKSGIVETVMAWAKFKQQAQLEKKCAGKKTSKLKGIPKLEDANDAGTRSSSECTLILTEGDSAKTLAVAGLGVVGRDKYGVFPLRGKVLNTREATVKQMMENAEINHIIQIVGLQYKKKYETVDDLKTLRYGKLMIMTDQDQDGSHIKGLIINFIHHNWPSLLRLNFLEEFITPIVKATKGKEEISFYSLPEYEEWKSNKDNWHTYKIKYYKGLGTSTSKEAKEYFSDMVRHRIKFKYEGDQDDRSIQMAFSKKAADQRKEWLTAWMLECKRRRELGLPEIYLYEKNTHSVNYSDFVNKELVLFSNADNERSIPSLVDGFKPGQRKVMFTCIKRNDKREVKVAQLAGSIAEHTAYHHGEVSLMSTIINLAQNFVGSNNINLLEPIGQFGTRLMGGKDAASPRYIFTKLSPLARALIHPHDDPLLKYLQEDNQKIEPEWYMPIIPLVLVNGAEGIGTGWSTKIPNYNPREIVDNIRKMIRGEEPKDMLPWFKNFRGTVEQLDATRYLCSGEVARLEDNQYEISELPIRTWTQSYKENVLE-ILQGNDKIPPIISDYREYNTDTTVRFVLNVLDNQVRHVEAE-GPHKIFKLQSSMSINTMVLFDANGVLKRYNSVMDVLQEFYELRLKFYEKRKLYMVGMLKAEANKLSNQARFIKEKCEGKLVVENKKRKDMINELITKGYESDPVKAWKKSQSREAAVEDEAAEESDAEEEQTDDAADFDYLLGMPMWSLTKERKEDLLKKEQEKKHELETLQAKSESDLWNHDLDVFIEELDKVEAKEREEERV--AKENLASKDKKAGGKKKKKYVDDALLEVMPSPMGERIQPRIDAEMRKKAEKSAAAGAAKAGRPKKRDNPDDDHDDLD---DLVSANTGKSLASRLGTSPD--LVKKRKAGRPTKKNGDGLKQTKINFPKKGKNPWS-SDSDADGGNS-------DSDLSTTNIETVPRSRAVEGKRAAAAKAKYNFSDDDEDDDDDIESLHSNDGVD----ERHVGPEKLSF-LDQTEDN----AAPDAPSPPKPAAKK---------SNAPKPKKANAHSDEDIVISDTDSEFGDFQQKKKKPSA--------AASTKQAASTADGSNASD 1480          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1067070037|ref|XP_018018367.1| (PREDICTED: DNA topoisomerase 2-alpha-like isoform X2 [Hyalella azteca])

HSP 1 Score: 1730.3 bits (4480), Expect = 0.000e+0
Identity = 882/1497 (58.92%), Postives = 1103/1497 (73.68%), Query Frame = 0

Query:   43 KAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ-------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLK----------IEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPK-----KRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS--------EVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKKPPAAA-TKKSKKTSQGSDYDD 1508
            +A     K+ +VE+ YQKK+QLEHI+LRPDTYIGSV  VTE+MW+ D  +++ V +EITYVPGLYKIFDEILVNAADNKQRD  MDTIKI+I+ E N I V+NNG GIPV MHKDEKMFVPT+IFGHLLTSSN+ND+E+KVTGGRNG+GAKLCN+FSTKFTVETA++E +K FKQ W  NMSKA + K+KDF+ G +YT+ITF+PDL KF M+ LD DTV LLSRRAYD+AASS GVKV+LNGKR+ VK+FKDYV+ ++K   D+ G+P+K +YE  G RWEVAV +S+ GFQ MSFVNSIATTKGGRHVDY++ MI +++ E L KKNK GV IK  QI+NHLW+F+N LI NPTFDSQTKENMTL    FGSKC+ + KF   + KSG+VE+V+AW+KFK   +++ K + KKT+KLKGI KLEDANDAGT++S +CTLILTEGDSAKTLAVAGLGVVGRDK+GV+PLRGK+LN REAT KQ++EN EIN +++I+GL YKKKY TVDDLK+LRYGKLMIMTDQDQDGSHIKGL+INFIHHNWPSLLRL FLEEFITPIVKATKG    SF+SLPE  EWKS+ DNWHTYKIKYYKGLGTSTSKEAKEYFSDM RH+I FK+E DQDD +I MAFSKKA DQRKEWLT WM ECKRR+ELGL EIYLYEK+T +V+YSDF+NKELVLFSN DNERSIPS+VDGFKPGQRKVMFTC+KRNDKREVKVAQLAGS+ E +AYHHGE SLM TIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT++SPL R + + +DD +L YL +DNQKIEPEWYMPIIP+VLVNGA+GIGTGW TKIPNYNPREIV N+  MI G EP+DM PWFK+F+G +E ++  RY+ +GE+A + +N+ E++ELP+R+WTQSYKE V+E +L G++KIP II DY+EY+TD TV+FV+ +  +++R  E + G H+ FKLQ+++S ++MVLFDA G L++Y +V+++++EF+ LRL FYEKRK Y+ GML+AE+ KLSNQARFI EKC+G LVVENKKRK +I EL  KG++SDPVK WK +Q           +     E   +   D+DYLLGM MWSLT+ERK++LL+++ EK  EL  L+  S+ DLW  DL  F+E+LD+VEAKE E+ERV  +KE+      K   K          +E +PSP+G R+ P I  E++ KA K+AAA + K  +  K++  + +    +        +  +SL+ +LG S +    KKR +   T K     K+ + +   KGK PW  SD+ ++  NS       DS       ++ P+S +          + Y        +    +  +S+D  D    +R   P    F LD ++++      P AP+  +P+                   KK    K KK    SD+D  I    ++      K KKP AAA TK++  T+ GS+  D
Sbjct:   29 QAAEPAGKKRTVEKTYQKKTQLEHIILRPDTYIGSVEKVTEQMWVFDTADEKFVQREITYVPGLYKIFDEILVNAADNKQRDPKMDTIKIEIDAENNVISVYNNGHGIPVVMHKDEKMFVPTMIFGHLLTSSNYNDDEKKVTGGRNGFGAKLCNIFSTKFTVETANKEYKKNFKQTWAVNMSKASDPKIKDFT-GEEYTKITFSPDLAKFGMDCLDRDTVDLLSRRAYDVAASSTGVKVYLNGKRVPVKSFKDYVELYIKDRTDDAGAPLKVVYEKPGPRWEVAVTISEKGFQHMSFVNSIATTKGGRHVDYITNMIVNSLTETLSKKNKSGVQIKNFQIKNHLWVFVNALIENPTFDSQTKENMTLQQKSFGSKCTLSDKFVAAVVKSGIVETVMAWAKFKQQAQLEKKCAGKKTSKLKGIPKLEDANDAGTRSSSECTLILTEGDSAKTLAVAGLGVVGRDKYGVFPLRGKVLNTREATVKQMMENAEINHIIQIVGLQYKKKYETVDDLKTLRYGKLMIMTDQDQDGSHIKGLIINFIHHNWPSLLRLNFLEEFITPIVKATKGKEEISFYSLPEYEEWKSNKDNWHTYKIKYYKGLGTSTSKEAKEYFSDMVRHRIKFKYEGDQDDRSIQMAFSKKAADQRKEWLTAWMLECKRRRELGLPEIYLYEKNTHSVNYSDFVNKELVLFSNADNERSIPSLVDGFKPGQRKVMFTCIKRNDKREVKVAQLAGSIAEHTAYHHGEVSLMSTIINLAQNFVGSNNINLLEPIGQFGTRLMGGKDAASPRYIFTKLSPLARALIHPHDDPLLKYLQEDNQKIEPEWYMPIIPLVLVNGAEGIGTGWSTKIPNYNPREIVDNIRKMIRGEEPKDMLPWFKNFRGTVEQLDATRYLCSGEVARLEDNQYEISELPIRTWTQSYKENVLE-ILQGNDKIPPIISDYREYNTDTTVRFVLNVLDNQVRHVEAE-GPHKIFKLQSSMSINTMVLFDANGVLKRYNSVMDVLQEFYELRLKFYEKRKLYMVGMLKAEANKLSNQARFIKEKCEGKLVVENKKRKDMINELITKGYESDPVKAWKKSQSREAAVEDEAAEESDAEEEQTDDAADFDYLLGMPMWSLTKERKEDLLKKEQEKKHELETLQAKSESDLWNHDLDVFIEELDKVEAKEREEERV--AKENLASKDKKAGGKKKKKYVDDALLEVMPSPMGERIQPRIDAEMRKKAEKSAAAGAAKAGRPKKRDNPDDDHDDLD---DLVSANTGKSLASRLGTSPD--LVKKRKAGRPTKKNGDGLKQTKINFPKKGKNPWS-SDSDADGGNS-------DSDLSTTNIETVPRSRAVEGKRAAAAKAKYNFSDDDEDDDDDIESLHSNDGVD----ERHVGPEKLSF-LDQTEDN----AAPDAPSPPKPA-------------------KKSNAPKPKKANAHSDEDIVISDTDSEFGDFQQKKKKPSAAASTKQAASTADGSNASD 1479          

BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1355946814|gb|PRD20904.1| (DNA topoisomerase 2-alpha [Nephila clavipes])

HSP 1 Score: 1711.43 bits (4431), Expect = 0.000e+0
Identity = 829/1314 (63.09%), Postives = 1038/1314 (79.00%), Query Frame = 0

Query:   50 KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGGLEKDL-----DYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAK--AAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSV--NDKGAKKRGSDSSTPKKRPQS-SPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSD 1353
            K+LSVERIYQKK+QLEHILLRPDTYIGSV PVT+ MW+  EEN  +VN+E+TYVPGLYKIFDEILVNAADNKQRDK+MD I+I+IN EKN+I ++NNG+GIPV  HKDEKMFVP+LIFGHLLTSSN+NDEE KVTGGRNGYGAKLCN+FSTKFTVETAS+  +K+FKQ W++NM+KA+E K+  F+ GSD+T ITF PDL KF M  LD D VA+++RRAYDIA  ++GVKV LN K++ VK+FKDY+  +L    D+ G P+K +++   ERWE+AV +S++G+Q +SFVNSIATTKGGRHVDY++  + S IAEV+KKKNKGG  +KP QI++HLW+F+NCLI NPTFDSQTKENMTL +  FGSKC  T KF  Q  K GVVES+++W +FKA  E++ K S KK +KLKGI KLEDANDAGTKNSIDCTLILTEGDSAK+LAV+GLGVVGRDK+GV+PL+GK+LNVREA+HKQI+EN EIN ++KI+GL YKKKY ++DDLK+LRYG+LMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKA+KG     F+SLPE  EWK+ T NW+ +K+KYYKGLGTST+KEAKEYFSDMKRH+I F +  ++DD+AI +AFSKK +D RKEWLT  MEE KRR+ELGL E+YLY K+T  V+Y+DF+NKEL+LFSN+DNERSIPS +DGFKPGQRKV+FTCLKRNDKREVKVAQL GSV E SAYHHGEA+L GTII LAQ+FVGSNNIN+LLP+GQFGTRL GGKD ASPRYIFT ++ +++ +F+  D  +L+YL DDN KIEPE+Y+PIIPMVLVNGA+GIGTGW TK+PNYNPR++V+N+  ++DG +P  M PW+K+F+G I+ +++Q ++V+GEIA +  NKIE+TELP+R+WTQ YKE V+EPM++G+EKIPA I DYKEYHTD TV+FVV M+ +K  QA  ++G H+ FKLQT+IS SSMVLFD  GCLRKY++  +++REF+ +R   YE+RK Y+ G+LEAESLKL NQARFILEK +  + +EN++R  +I+ LQ +G+DSDPVK WK AQK   D +  SE   EK++     DYDYLLGM +WSLT+ERKD+LL+++DEK +EL  L+  S  DLWR DLKEFME+LD+VE ++ EDE  + S + +K   GKKK   E  PSP G R+ P I ++ K K  K      + +KG K    ++KE +          D KD+  SLS++L   V     GAK      +  KKR    SPK   + PW D   ++E  + +   D ++ G D
Sbjct:   24 KKLSVERIYQKKTQLEHILLRPDTYIGSVEPVTQTMWVY-EENVGLVNREVTYVPGLYKIFDEILVNAADNKQRDKSMDLIRIEINPEKNKICIWNNGQGIPVVEHKDEKMFVPSLIFGHLLTSSNYNDEERKVTGGRNGYGAKLCNIFSTKFTVETASKAYKKQFKQTWKDNMTKAEEPKINPFN-GSDFTCITFYPDLAKFKMTVLDKDIVAIMTRRAYDIAGCARGVKVILNDKKLPVKSFKDYISLYLSEKTDDNGDPLKPVHDVVNERWEIAVTLSNSGYQHVSFVNSIATTKGGRHVDYITDQLTSKIAEVIKKKNKGGKDVKPFQIKSHLWVFVNCLIENPTFDSQTKENMTLQIKNFGSKCQLTEKFINQAMKCGVVESIMSWIRFKAQTELEKKCSGKKHSKLKGIPKLEDANDAGTKNSIDCTLILTEGDSAKSLAVSGLGVVGRDKYGVFPLKGKMLNVREASHKQILENAEINNIIKIMGLQYKKKYESIDDLKTLRYGRLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKASKGKEEKCFYSLPEFEEWKTETPNWNKFKVKYYKGLGTSTAKEAKEYFSDMKRHRIRFHYTGNEDDNAIQLAFSKKMVDMRKEWLTNGMEERKRRRELGLPELYLYGKNTTAVTYNDFVNKELILFSNMDNERSIPSAIDGFKPGQRKVLFTCLKRNDKREVKVAQLVGSVAEQSAYHHGEAALAGTIIGLAQDFVGSNNINVLLPVGQFGTRLQGGKDAASPRYIFTMLNSVSKHLFSGLDSPLLHYLYDDNLKIEPEYYVPIIPMVLVNGAEGIGTGWSTKVPNYNPRDLVKNIYKLLDGEDPLPMNPWYKNFRGQIDRLDDQHFLVSGEIAILENNKIEITELPIRTWTQQYKENVLEPMVHGTEKIPAFITDYKEYHTDTTVRFVVTMTPEKFNQAR-EQGFHKVFKLQTSISLSSMVLFDHLGCLRKYDSPEQVLREFYEVRFALYERRKEYMVGVLEAESLKLKNQARFILEKIENKISIENRRRAEMIKTLQDRGYDSDPVKAWKEAQKENIDEEKASESDDEKEIEPSGPDYDYLLGMTLWSLTRERKDDLLKKRDEKIKELDDLKSKSPSDLWRNDLKEFMEELDKVEKEQKEDE--VGSSKLKKKTTGKKKFADEDKPSPFGERINPQIDDDYKKKWEKKPTVPGEVKKGRKKKLPDIKEEDG--------DDIKDV--SLSDRLNTPVKKGKGGAKTAPKQPAGEKKRGAGKSPKKPKRNPWSDDSESNEELSGIS--DMEELGDD 1320