low quality protein: myosin heavy muscle, maker-scaffold1366_size45417-snap-gene-0.5 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of low quality protein: myosin heavy muscle vs. L. salmonis genes
Match: EMLSAG00000008263 (supercontig:LSalAtl2s:LSalAtl2s49:907807:914722:1 gene:EMLSAG00000008263 transcript:EMLSAT00000008263 description:"augustus_masked-LSalAtl2s49-processed-gene-9.4") HSP 1 Score: 252.292 bits (643), Expect = 1.393e-70 Identity = 137/294 (46.60%), Postives = 193/294 (65.65%), Query Frame = 0
Query: 1260 MSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYEDAANHLN 1553
MSEQ+DQL K+K K E DK + + +E+R +MD + KAS EK N+ + E+SK+ E ++ L D++N KK + EN +++RQ+EE+D N + L+K K L NQL++ KR+A+DE+KER LLG++RNLEHE D M EEE AKD+ R K + LWRQ YE EG+AK EELE +K+KLQARL TVE N+K + L+K K+ LQ +I+ M+++ D A + MEKK R FDK+I +WK+K D L ELD +Q +CR +T+LF+VK YE+ L+
Sbjct: 1 MSEQIDQLNKMKQKAEKDKHSRRLQIDEVRAAMDTINNEKASIEKQNRIATSQYNEVSKKCEEANMSLGDLENSKKKIIMENADLLRQIEEIDNNNNTLSKLKSNLMNQLDEQKRIADDESKERNFLLGKFRNLEHEVDTMREQVEEEGQAKDNALRTLSKTLSDVQLWRQRYEKEGLAKAEELEAAKMKLQARLGXATATVETLNHKAMSLEKEKSHLQCQIEEMSTNADAAAQRCHLMEKKARNFDKVIVEWKNKTDSLQAELDRNQIDCRTFSTDLFKVKTIYEETQQQLD 294
BLAST of low quality protein: myosin heavy muscle vs. L. salmonis genes
Match: EMLSAG00000005286 (supercontig:LSalAtl2s:LSalAtl2s282:243643:310504:-1 gene:EMLSAG00000005286 transcript:EMLSAT00000005286 description:"maker-LSalAtl2s282-snap-gene-3.34") HSP 1 Score: 256.529 bits (654), Expect = 1.014e-68 Identity = 196/804 (24.38%), Postives = 359/804 (44.65%), Query Frame = 0
Query: 131 EQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFT--HEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQK--GREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQ-------------NLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNG------FEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGI--DWAMVDFGMDLQACITMFEK-P--------------MGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSDKNAHFAIVHYAGT--VSYNLSGWLEKNKDPLNDTVVELLKCGSNNLV---------VHIFADHPGQSPLPED-----------KTKKGKKAKGSGA------KTVSTFYKTQLDSLMSTLHATEPHFIRCIVP-------------------------------------NGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKK--------ITELICNDFIDKERFRIGNTKVF 821
+ V + NPP ++K ED++ L YLNE+SVL L+ RY LI+TY+G +++NP IY+ ++++ + ++PPH+++++ +YR+MLS +QS++ G SG+GKT N K ++SYF K +K+ +++ +LEAFG+++T N N+SRF + I + F+ASG L I+ + EK+R+ + + E +++IFY ++ DS +K L +V+Q + DDK + + A +L + E +++ +AA+ H+G + G+ P + +K LG E + + I GT + NL ++ G GLY VF ++ N+ + DP + +LD GF+ G FE++C N+ E+LQ F+ + E+Y+ E + D +D ++ +++ +K P G+L +L+EE+LFP A+D++F ++L + + + +K F + H+ GT V YN GWL +++ V LLK N L ++ + PG + + + ++A +G +++ K LD ++ L T+ HF++C +P N N + ++ LV Q+ +L+ +R+ G+P +PY F R+ +L N A G +P + ++E+ +D +R+GN++ +
Sbjct: 450 DDVEKANPPGYDKVEDLAQLRYLNESSVLNTLRQRYGNNLIHTYAGPSLISINPMSPLAIYSDKVIQMFKECKTEDMPPHIYSVAQLSYRSMLSTRKDQSIVFIGRSGSGKTTNVKHMLSYFT-------KTSGNKQVITTEKVNAIYVLLEAFGHSRTNLNSNASRFTELISLDFDASGGLISASIQAMMXEKTRVVRRPDGEPNFNIFYHLLMGSDSS--TRKDLMLDNLNEPNLFVTQ----LQKPDDKSKVIWNKIQSAIKVLGISSHEAHSLWSVLAAIFHLGTASVTRGTMGKSLFARP---QAGQKAAQCLGTSSEELAR-----NIFQGTSSTSIRSKSRDSSFRSSDLTNLPDGIDSLHGFVIGLYQEVFNTLIYMINRCISDPIANTFNSVYILDTPGFQNPASCGRIKGACFEELCHNYVQERLQLLFHKRNISSQYEKYLQEQVEPDVGDLDDIGSPESMVSLLDKQPGTRSSQTDLSSTERRGLLWLLDEEALFPGASDETFVERLLLQYNQRGSDELLKRGLGEK--QFVLQHFQGTNPVLYNAEGWLSSSRED-----VALLKAAGNILTESNTKSISELYSSSRGPGSTSVSGSVAGLEGSISLRRASSIRRAFTTGGHAGIKRHSIALQQKFLLDGVLEQLRRTKVHFVQCFLPQHGAGLCDIVGRSASKGSNSSTSSSIGSGDSLSLEANNSNVL---MNIPLVRSQVRGGQILDSVRLFKNGYPESIPYALFWRRFHVLGEN--ANQGLTEPPRNVDHLKTAVSEIFSFLDLDSSAYRLGNSQRY 1220 HSP 2 Score: 68.5514 bits (166), Expect = 2.206e-11 Identity = 31/88 (35.23%), Postives = 50/88 (56.82%), Query Frame = 0
Query: 820 VFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIK 907
VF RAGV +LE+ RDD + + LQ C G+L RK IQ +++N RKF+ +R W W+ ++ K P++ + E+++K
Sbjct: 1534 VFLRAGVYAHLEQERDDTLQDQITNLQALCRGYLARKK----------IQKLKKNVRKFMGVRGWHWWRLLIKITPMLNVHRTEDQLK 1611
BLAST of low quality protein: myosin heavy muscle vs. L. salmonis genes
Match: EMLSAG00000004035 (supercontig:LSalAtl2s:LSalAtl2s215:559787:562711:1 gene:EMLSAG00000004035 transcript:EMLSAT00000004035 description:"augustus_masked-LSalAtl2s215-processed-gene-5.1") HSP 1 Score: 209.149 bits (531), Expect = 4.647e-55 Identity = 218/818 (26.65%), Postives = 396/818 (48.41%), Query Frame = 0
Query: 1167 ETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMA--------------SHVDNANMQYSQMEKKIRQFDKII--GDWKHKADG-----LTEELDHSQ--------------KECRNVATELFRVK--------------NGYEDAANHLNELSQARQEIERRLAEKEEEFDAVRRSHQKG------------------IEMMQSNL-EAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQ-NQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGR 1915
E Q RV L+++ E+E+ R + ER+K DL ++ L+ERL +A G Q+++N+KRE E+ +LRK LEE + E I LKK+HQ A+ E+ EQ+D + + K KV +K+ +Q E E L MD K + K + + + E + ++ +L + + N E R+L E+ I K ++ +QL D + ++ ++ + R LE + ++ +E + DL +Q + E W++ YE E +IE+++ K K +++A+ + ++ +KL +++AK LQ E+ ++ S ++ + + + K+R+ + D + KA G L+ ++D + ECR + +EL +K + E+ A E R E R+AE E E +R QKG IE + + L E E + K E ++MKKK+ + ELE +L AN ++ Q N++K Q N+I + +D K + + + ++ ++ L ++ ++A R E ++ E N +++L+ N S+ K +LE E+ + D ++ E + +D+A KA DA LR E + +++ ++ L+ +VK L I++++ E A+ R+ +++E RI+ELES E+ ++ L K ER +KE+ Q EEDRK +Q+ +++L K++ +K Q+EE E I+ N+ + +K Q E E+A RA+ +E +L+ +R R
Sbjct: 60 EEQIRV--LQDDYESEQHLRRRVEREKQDLQMQILSLTERLTQAEGGAECQIDINRKREAEMSQLRKLLEELHTESEHNIHELKKRHQTAMMELQEQIDSVARSKDKVSKEKSKLQVEIESLLAQMDVFSSEKTTVRKTIERLEIQVNEYNCKIDVMCKELXAVTSERNSLKLSNDESNRRLNELKMAIENAGLDKNKVASQLRDLQIQLDNASRGKNQAESRIVALETQLKSISVEIQEYKETRIDLEKQLGHFKSEGLDWKKKYENEARLRIEDIDCLKKKFGSQIADLQDKLDAVLSKLKGMEQAKNRLQIEVQTLIKDLGISQSTVKDFQSRLNISEKKCEDLAIKLREMTNMYEKADNEVKARGQELMRLSNDMDRLKMDNGVLLKDRSKLDDECRMLKSELDALKRQLHASEQENRKMAHDREELAKAFKEADVGRNRAENRIAELEAELKRLRGDSQKGLLMKEDEFNSMKKKLVSEIESLSTRLHETENRLKNEVEKMKKKMAXTITELEMSLNSANQSNVSLQ-NVNKTQVNKIMELTSLYDSVCKKLDLTAGDASSLCKKMALTESELHSMKSAYGNVEKAHRVTEAKVQELNVKINELTQVNISINNTKVKLEKELGTVTSDYTDIARELKLADDRANKASHDAKHFESLLRDESAKLVRIDHAKQALECEVKSLTIKMEEIETTAITTSRRTIKQLEIRIEELESMFSKEKVCHVETATMLHKKERVVKELLCQSEEDRKNILILQESLDRLNDKIKMYKHQLEEQECISNSNIMRVKKFQRELEDASGRAEEAESSLNSFRSRXR 874
BLAST of low quality protein: myosin heavy muscle vs. L. salmonis genes
Match: EMLSAG00000008139 (supercontig:LSalAtl2s:LSalAtl2s485:3423:47423:-1 gene:EMLSAG00000008139 transcript:EMLSAT00000008139 description:"maker-LSalAtl2s485-augustus-gene-0.6") HSP 1 Score: 174.866 bits (442), Expect = 5.104e-44 Identity = 188/716 (26.26%), Postives = 308/716 (43.02%), Query Frame = 0
Query: 140 KFEKCEDMSNL-TYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVP--PHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYF---ANVGAREEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHL-SEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDP------TM--------------------------KKVVFIGVLDIA---------------GFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEK-PMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSDKNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELL---KCGSNNLVVHIFADHP----GQSPLPEDKTKKGKKAKG-------SGAKTVSTF---YKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAIL 783
K + ED+ L +NE ++L L+AR+Q + +T G ++VNPY T L L +N+ +L + R G+ Q+++++G SG+GK+ + ++ AN G++E K AS +L + G AKTA N SSR G FI + + G L I Y L++SR+ +E++YHIFYQM+ L D ++ L I D ++++ G D E E IL + V + +A+++ +G + F + G+ + E E+ V +LGV + + V + V +++ + +A+ LY R IV + N +L P TM K + +L+ A GFE K + E +CIN C+E +Q F+N H+F E EGI +V +D CI + G+L++L+ E + +++ K+K H K D + FAI HYAG V Y+ S +L+ N+D + D ++ + C H+F + Q +P + + +G + VST + T+LD+L+ TL +PHF+RCI PN + E D LV Q+ +LE + + GFP+RM + F +RY L
Sbjct: 259 KLSRIEDLIRLPCTVNEDTILKALQARFQNRSYFTNVGPIVLSVNPY------TEVGNPLTLDSTKNQAANSKYLQKVVKETVRLQSETGYPQALIVSGGSGSGKSYTSMILLRQLFEQAN-GSQETDTFKHLAASFS--------VLRSLGMAKTASNRESSRIGHFIEVQV-SDGALYRTKIHCYFLDQSRVVQPLPMEKNYHIFYQMLAGLSQ--DERRSLGLDGLTIRDLNFLNSGDYRQDEAQDAERFEEWKVNLGILGIPFMDVVRV---LASILLLGNIDFAE-GKGLEVEVVGREELNSVASLLGVPSSHLWQGLTTRTYSVRGQLVKSMSDINMAAATRNALAKALYCRTVATIVRRAN-SLKRPVGAMSGTMSSDSNESVQHDVASHQASTVGTAGSRKSSKSMAILNHAVRHATDGFIGILDMFGFEDSKPSRLEHLCINLCSETMQHFYNTHIFKSSIESCRDEGIITDLVVDYVDNVPCIDLISSLRTGLLSMLDVECSV-RGCPETYVQKVKIQH--KDNKKLXEPKHCDISRTFAIHHYAGQVVYDTSYFLDTNRDVIPDDLLCVFDRDTC-QFGFATHLFGNEIKALRSQDTIPRGVSFRISPTSSSTSPELLNGDEPVSTLTQDFHTRLDNLLRTLVHAKPHFVRCIKPNNKESSTEFDRTLVAQQIRSLQILETVNLMSGGFPHRMRFKAFNTRYRCL 947
BLAST of low quality protein: myosin heavy muscle vs. L. salmonis genes
Match: EMLSAG00000002471 (supercontig:LSalAtl2s:LSalAtl2s147:318144:322176:1 gene:EMLSAG00000002471 transcript:EMLSAT00000002471 description:"maker-LSalAtl2s147-augustus-gene-3.7") HSP 1 Score: 151.754 bits (382), Expect = 2.967e-42 Identity = 76/135 (56.30%), Postives = 96/135 (71.11%), Query Frame = 0
Query: 92 KKSYWVPDDQGGFVEGLVQS---DDGKKAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNML 223
K+ WVP D GFV + D+ + IV G + K + + ++NPPKF+K EDM+ LT LNEASVL N+K RY + LIYTYSGLFCV VNPYKR PIYT ++LY GK+R+EVPPH+FAI+DTAYR+ML
Sbjct: 33 KRLVWVPHDSHGFVAASXVTNMKDEVEVEIVESGKRIEVSK-DDIQKMNPPKFDKEEDMAXLTCLNEASVLHNVKERYYSGLIYTYSGLFCVVVNPYKRLPIYTEKIIELYKGKKRHEVPPHVFAITDTAYRSML 166
BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Match: gi|110825729|sp|P05661.4|MYSA_DROME (RecName: Full=Myosin heavy chain, muscle) HSP 1 Score: 1930.22 bits (4999), Expect = 0.000e+0 Identity = 1036/1946 (53.24%), Postives = 1355/1946 (69.63%), Query Frame = 0
Query: 54 MPGNVKLGSSNEPDPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIV-LIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKSK-KASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSDKN--AHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITE-LICNDFIDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYEDAANHLNELSQARQEIERRLAEKEEEFDAV----RRSHQKGIEMMQSNLEAE----------------------MKAKAEAQRMKKKLEADVMELESALEHANVTHQ----------------------------------------------ENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRATTPNPG 1922
MP V +N+ D DP PYL VS+E +R D KPYD KKS W+PD++ G++ G +++ G V L G E + KSE+V +VNPPKFEK EDM+++T LN VL NL+ RY AKLIYTYSGLFCVA+NPYKR+P+YT+ K+Y GKRRNEVPPH+FAISD AY +ML+N NQSMLITGESGAGKTENTKKVI+YFA VGA ++ +K K SLEDQ+VQTNP+LEAFGNAKT RNDNSSRFGKFIRIHF +GKLAG DIETYLLEK+R+ QQ +ERSYHIFYQ+M S P +K +C L+++IYDYH VSQGK +V SIDD E+ T +AF+IL FT++E ++Y+ AAVMHMG MKFKQ+GREEQ E D E+ +V + G D + K KP+IKVG E+VT+G+N+ Q T ++ + +G++DR+F+++V+KCN+TL D K+ FIGVLDIAGFEIF+YNGFEQ+CINF NEKLQQFFNH MFV+EQEEY EGI+W +DFGMDL ACI + EKPMGIL+ILEEES+FPKATD++F +KL HLGKS F K AHFAI HYAG VSYN++GWLEKNKDPLNDTVV+ K N L++ IFADH GQS E K G A TVS+ YK QL+SLM+TL +T+PHF+RCI+PN K PG +D+ LV+HQLTCNGVLEGIRIC +GFPNRM YP+F RY IL+ I L PKK ++ LI + ++++ +R+G+TKVFFRAGVLG +EE RD+ + K++ ++Q G+L RK F+K ++QR ++V+QRN RK+L LR W W+ + QK KPL+ + IE+EI LE+ A KA +E R+ LE N +L + A++ + E+G L YQER+AK QK E +L D Q++L +E L QK+ + E+ ++KD+ +LE + KAE +K T+DH++RNLND+IA+ DE+++KLNKEKK E N K E+L +AEDK+NHLN VK+KLEQT+D+LEDSLEREK+ + DV+K +RKVEGDLK+ QE V ++ER K+ELE +QR+++++ + A+LE EQ K QR IKE QAR+EELEEE+EAERQARAKAE+Q+ DL REL+EL ERLEEA GAT+AQ+ELNKKRE EL +LR+DLEEA IQ EST+ +L+KKH DA+ EM+EQ+DQL KLKAK EHD+ E + R + D L R KA+ EK K Q L E+ ++ E + L D D KK EN +++RQLEE + +S L+K KI LT QLED KR+A++E++ER +LLG++RNLEH+ D + EEE K DL RQ KA EA +WR YE +G+A+ EELE +K KLQARLAE E T+E+ N K I L+K K L E++ + VD AN + EKK + FDKIIG+WK K D L ELD SQKECRN +TELFR+K YE+ L + R+E + E ++ D + R H+ IE + LEAE ++A+ E +++++++ + E E E+ HQ E Q+NI +YQ Q++D Q ++EQ+ + ARE + +ERRA+ LQN LEE+RTLLEQADR RR AEQEL++ +E ++++S QN S+ A KR+LE E+ L DLDE+ E +E+KAKKAM+DAAR+++ELR EQD Q E RK L+ Q+K+LQ+RLD+AE NA+K G+KA QK+E R++ELE+ELDGEQRR AD+ KNL+K+ER++KE+ FQ EEDRK HE MQDLV+KLQ K++ +K+QIEEAEEIAA+NL KFRKAQ E EEAEERADL+EQA+SK+R GRA + G
Sbjct: 1 MPKPV----ANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMS--GSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETL-DTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQSGGGEQAKGGRGKKGGGFA-TVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPRGIKDLDC--PKKASKVLIESTELNEDLYRLGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAV---RRENKNLADEVKDLLDQIGEGGRNIHE--IEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGRAGSVGRG 1931
BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Match: gi|127773|sp|P24733.1|MYS_ARGIR (RecName: Full=Myosin heavy chain, striated muscle) HSP 1 Score: 1702.57 bits (4408), Expect = 0.000e+0 Identity = 911/1923 (47.37%), Postives = 1287/1923 (66.93%), Query Frame = 0
Query: 67 DPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLI--GHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGA----REEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSDKN---AHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNG---YEDAANHL-------------------------NELSQARQEIE---------------------------------------RRLAEKEEEFDAVRRSHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRV 1912
DPD YLAV + ++ +D KK+ WVPD++ GF +QS G + V I +T K + + +NPPKFEK EDM+N+TYLNEASVL+NL++RY + LIYTYSGLFC+AVNPY+R PIYT + + Y GKR+ E+PPHLF+++D AY+NM+++ NQS LITGESGAGKTENTKKVI Y A V ++E+ K+ SLEDQI+Q NP+LEA+GNAKT RN+NSSRFGKFIRIHF +GK+AG DIETYLLEKSR+T+QQ ER+YHIFYQ+ ++ P++ V ++ D Y +++QG +V +IDD E+ + EAF+IL FT+EE +++K A+++HMGEMKFKQ+ REEQ E D +A+KV + G++ ++KA KPK+KVGTE VTKGQN++Q +V +A+ LYDR+F ++V + N+TL D K+ +IGVLDIAGFEIF +N FEQ+CIN+ NE+LQQFFNHHMF+LEQEEY EGI W +DFGMDLQ CI + EKPMGIL+ILEEE +FPKA DKSF+DKL NH+GK+ F K + N AHF + HYAG V Y+++GWLEKNKDP+N+ VV LL LV +F ++P K KK K S +T+S ++ L+ LM L++T PHF+RCI+PN K PG +D+ LVLHQL CNGVLEGIRIC +GFP+R+ Y EF RY+IL N I Q G D K ++E I +D +R+G TKVFF+AGVLG LEE+RD+ + K++ Q G+L RK ++K + QR + VIQRN RK+L LRNW W+ + K KPL+ + EEE+K +K E ++ LE++N L E + +++T + + +ER K QKA +E ++ + +++L E L+ K+ +E++ +++KD+ +LE+ L KAE +K +D+++ L +I+ DE + KLNKEKK ++E N K S+ L + EDK NHLN +K+KLEQ +D+LED+LEREK+ + DV+K +RKVE DLK QE V ++ER KRELE V+R+E +I +N++LE EQ ++ QR IKE QAR+EELEEELEAER ARAK E+Q+ +L REL+EL ERL+EA GAT+AQ+ELNKKRE EL ++R+DLEEA++Q E+ I +L+KKHQDA EM++Q+DQL K+K+K+E DK ++RE ++L M +++K +EK K F++ + +L+ R+ + + ++ + + AEN ++ RQLE+ + +S+L+K K QL++QLEDA+R E+E + R L RN+ + D + EEE +K D+ RQ KA +E WR +E EG + EELE+ K KL +L+E E T E N K L+KAK+ LQQE++ M+ VD AN +QMEKK R FDK +W+ K + L EL++SQKE R + EL+R+K Y+D+ L +EL +AR+ +E +R+ EKEEEFD RR+HQ+ +E MQ++LEAE K KA+A R+KKKLE D+ ELE AL+ +N E ++ + +YQ QIR+ Q ++EQ+ + ARE+ AERR + +EE R LEQA+RAR+A++ EL++ N+ +++L+ Q S+ KR+LEG+++ ++ DLDEM E +++ KKAM DAAR+++ELR EQD + +E RK L++QVK+ QIRLD+AE +++K G+K QK+ESR+ ELE+ELD EQRR A++ KN++K +R++KE+ FQ +EDRK E +Q+L++KL K++ FK+Q+EEAEEIAA+NL K+RKAQ E EEAEERAD ++ L K+R
Sbjct: 7 DPD-FQYLAVDRKKLMKEQTAAFDGKKNCWVPDEKEGFASAEIQSSKGDEITVKIVADSSTRTVKKDDIQSMNPPKFEKLEDMANMTYLNEASVLYNLRSRYTSGLIYTYSGLFCIAVNPYRRLPIYTDSVIAKYRGKRKTEIPPHLFSVADNAYQNMVTDRENQSCLITGESGAGKTENTKKVIMYLAKVACAVKKKDEEASDKKEGSLEDQIIQANPVLEAYGNAKTTRNNNSSRFGKFIRIHFGPTGKIAGADIETYLLEKSRVTYQQSAERNYHIFYQICS--NAIPELNDVMLVTPDSGLYSFINQGCLTVDNIDDVEEFKLCDEAFDILGFTKEEKQSMFKCTASILHMGEMKFKQRPREEQAESDGTAEAEKVAFLCGINAGDLLKALLKPKVKVGTEMVTKGQNMNQVVNSVGALAKSLYDRMFNWLVRRVNKTL-DTKAKRNYYIGVLDIAGFEIFDFNSFEQLCINYTNERLQQFFNHHMFILEQEEYKKEGIAWEFIDFGMDLQMCIDLIEKPMGILSILEEECMFPKADDKSFQDKLYQNHMGKNRMFTKPGKPTRPNQGPAHFELHHYAGNVPYSITGWLEKNKDPINENVVALLGASKEPLVAELF-----KAPEEPAGGGKKKKGKSSAFQTISAVHRESLNKLMKNLYSTHPHFVRCIIPNELKQPGLVDAELVLHQLQCNGVLEGIRICRKGFPSRLIYSEFKQRYSILAPNAIPQ-GFVDGKTVSEKILAGLQMDPAEYRLGTTKVFFKAGVLGNLEEMRDERLSKIISMFQAHIRGYLIRKAYKKLQDQRIGLSVIQRNIRKWLVLRNWQWWKLYSKVKPLLSIARQEEEMKEQLKQMDKMKEDLAKTERIKKELEEQNVTLLEQKNDLFLQLQTLEDSMGDQEERVEKLIMQKADFESQIKELEERLLDEEDAAADLEGIKKKMEADNANLKKDIGDLENTLQKAEQDKAHKDNQISTLQGEISQQDEHIGKLNKEKKALEEANKKTSDSLQAEEDKCNHLNKLKAKLEQALDELEDNLEREKKVRGDVEKAKRKVEQDLKSTQENVEDLERVKRELEENVRRKEAEISSLNSKLEDEQNLVSQLQRKIKELQARIEELEEELEAERNARAKVEKQRAELNRELEELGERLDEAGGATSAQIELNKKREAELLKIRRDLEEASLQHEAQISALRKKHQDAANEMADQVDQLQKVKSKLEKDKKDLKREMDDLESQMTHNMKNKGCSEKVMKQFESQMSDLNARLEDSQRSINELQSQKSRLQAENSDLTRQLEDAEHRVSVLSKEKSQLSSQLEDARRSLEEETRARSKLQNEVRNMHADMDAIREQLEEEQESKSDVQRQLSKANNEIQQWRSKFESEGANRTEELEDQKRKLLGKLSEAEQTTEAANAKCSALEKAKSRLQQELEDMSIEVDRANASVNQMEKKQRAFDKTTAEWQAKVNSLQSELENSQKESRGYSAELYRIKASIEEYQDSIGALRRENKNLADEIHDLTDQLSEGGRSTHELDKARRRLEMEKEELQAALEEAEGALEQEEAKVMRAQLEIATVRNEIDKRIQEKEEEFDNTRRNHQRALESMQASLEAEAKGKADAMRIKKKLEQDINELEVALDASNRGKAEMEKTVKRYQQQIREMQTSIEEEQRQRDEARESYNMAERRCTLMSGEVEELRAALEQAERARKASDNELADANDRVNELTSQVSSVQGQKRKLEGDINAMQTDLDEMHGELKGADERCKKAMADAARLADELRAEQDHSNQVEKVRKNLESQVKEFQIRLDEAEASSLKGGKKMIQKLESRVHELEAELDNEQRRHAETQKNMRKADRRLKELAFQADEDRKNQERLQELIDKLNAKIKTFKRQVEEAEEIAAINLAKYRKAQHELEEAEERADTADSTLQKFRA 1919
BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Match: gi|74788753|sp|Q60LV4.1|MYO3_CAEBR (RecName: Full=Myosin-3; AltName: Full=Myosin heavy chain A; Short=MHC A) HSP 1 Score: 1628.22 bits (4215), Expect = 0.000e+0 Identity = 898/1952 (46.00%), Postives = 1267/1952 (64.91%), Query Frame = 0
Query: 63 SNEPDP---DP-MPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKA-IVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKSKKA-----SLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSDK--NAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCG-SNNLVVHIFADHPGQSPLPED----KTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKK----ITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLI-------GMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADG----------------------------LTEELDHSQKECRNVATELFRVKNGYEDAANHLNEL---------------------------------------SQARQEIERRLAEKEEEFDAVRRSHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRATT 1918
S PD DP P+L +S E K +P+D KK+ W+PD + GFV +QS G + +V + + T K +Q ++NPPKF+K EDM+NLT+LNEASVL NLK RY+ +IYTYSGLFCV +NPYKR PIY+ + +K ++GKRRNE+PPHLFA+SD AYRNM+ + NQSMLITGESGAGKTENTKKVISYFA VGA + K+ +LE+QIVQTNP+LEAFGNAKT RN+NSSRFGKFIR HF+ SGKLAG DIE YLLEKSR+ Q ER YHIFYQ+M D+ ++ L+ DI YH+ SQ + ++ +DDKE++ T EAF+I+ F ++ET+++Y+S A +MHMGEMKFKQ+ REEQ EPD E A +LG++ E +KA KP+++VGTEWV KGQNL+Q + AV+G+A+ +Y R+F++I+ +CN+TL +++ FIGVLDIAGFEIF N FEQ+ INF NE+LQQFFNHHMFVLEQEEY EGI W +DFG+DLQACI + EKP+GI++IL+EE + PKATD ++ KL HLGK NF K K +AHFAIVHYAGTV YN + +LEKNKDPLNDT V LLK NNL++ I+ D+ Q E ++ GK+ K S TVS Y+ L++LM+ L+ T PHFIRCI+PN K G IDS LVL+QLTCNGVLEGIRIC +GFPNRM YP+F RYAIL A+ DPKK I + I ND + E F+IG TK+FF+AGVL LE++RD+I+ ++V Q +L + + +R +Q+ + ++QRN R + +LR W WF + K KP++ M + E+I+ LE+A + A R +LE + L E+ A+ +ETE+ +L +ER+ K KA E +L+D +LE +R+ L QK+ E E+ +K + +LE L KAE EK +RDH++R+L D++AN DE ++KLNKEKK+ +E+N K +EDL S EDKVNHL +++KLEQ MD+LE++++REKR ++D++K +RKVEGDLKV QE + E+ + K+++E+ ++R+E D+ + +L EQ AK QR IKE QAR+ ELEEELE+ER +R KA+R +++L REL+E+SERLE+ G + AQ+E NKKRE E+ +LR++ EE + E+ + SL+K+ DA+ E++EQ++ L KLKAK + ++ +QR+ EE + + D VR++ EK+ K + EL + E S QL D + ENG++ R LEE+D ++ L++ K L +QL++ +R E+E++ERQ+L +NLEHE + + +EE +K DL RQ K E W+ ++ EG+ K+EE+E +K LQ ++ E T E K+ +K + L Q++D S V+ A Q + EK RQF+KI+ +WK K D L E LD +++E +++A E+ + + + + EL SQ R EIE+R+ EKEEEF+ RR+HQ+ +E MQ+ LEAE K K EA R+KKKLE+D+ +LE AL+HAN + + Q+ I KY +R+ Q++ ++EQ+ K RE L +E+R LQ +E E A+RARR AE + E E +DLS Q SL +R+LEGE+ + +L+E+ E +++ +KA DAAR++EELR EQ+ + H+E RK L+ Q+K++QIRLDDAE A+K G+K ++E+RI+ +E ELDGEQRR D+ KN +K ER++KE++FQ E++K E + +LV+KLQ KL+ FK+Q+EEAEE+AA NL K++ Q + E+A+ERA+++E ALSK R RA+
Sbjct: 2 SGNPDAFENDPGFPFLGMSREAKAASAARPFDSKKNCWIPDPEDGFVAAEIQSTTGDQVTVVTVKGNQITVKKDQCQEMNPPKFDKTEDMANLTFLNEASVLGNLKDRYKDLMIYTYSGLFCVVINPYKRLPIYSESVIKHFMGKRRNEMPPHLFAVSDEAYRNMVQDKENQSMLITGESGAGKTENTKKVISYFAIVGATQAAAGGKKEEGKKGGTLEEQIVQTNPVLEAFGNAKTVRNNNSSRFGKFIRTHFSGSGKLAGGDIEHYLLEKSRVVRQAPGERCYHIFYQIMSGNDAS--LRGKLKLNNDITYYHFCSQAELTIEGMDDKEEMRLTQEAFDIMGFEDQETMDLYRSTAGIMHMGEMKFKQRPREEQAEPDGEEDALNAAAMLGINAEEFLKALTKPRVRVGTEWVNKGQNLEQVSWAVSGLAKAIYARMFKWIINRCNKTLDAKEIERKHFIGVLDIAGFEIFDLNSFEQLWINFVNERLQQFFNHHMFVLEQEEYKREGIAWTFIDFGLDLQACIELIEKPLGIISILDEECIVPKATDMTYAQKLLDQHLGKHPNFQKPKPPKGKQGDAHFAIVHYAGTVRYNANNFLEKNKDPLNDTAVALLKHSVDNNLMLDIWQDYQTQEEAAEAAKAGQSGGGKRGKSSSFATVSMIYRESLNNLMNMLYQTHPHFIRCIIPNEKKASGVIDSALVLNQLTCNGVLEGIRICRKGFPNRMLYPDFKHRYAILAADAAK---DSDPKKASVGILDKIANDGNLTDEEFKIGETKIFFKAGVLAKLEDLRDEILSRIVTMFQSRIRSYLAKAEVRRRYEQQTGLLIVQRNVRAWCTLRTWEWFKLFGKVKPMLKAGKEQEAMGELAEKIQKLEEAVQRGEIA-------RSQLETQVADLVEEKNALFLSLETEKANLADAEERNEKLNQLKATLESKLTDITGQLEDMQERHEDLTRQKKKTEQELSDTKKHVQDLELTLRKAEQEKQSRDHQIRSLQDEMANQDESVAKLNKEKKHQEESNRKLNEDLQSEEDKVNHLEKIRNKLEQQMDELEETIDREKRSRSDIEKSKRKVEGDLKVAQENIDEITKQKQDVENTLKRKEDDLHHASTKLAEEQALAAKLQRQIKELQARIAELEEELESERNSRQKADRTRNELQRELEEISERLEQQGGFSAAQLEANKKREAEIAKLRREKEEDALNHETAVSSLRKRQVDAVAELTEQLETLQKLKAKGDAERAKLQRDLEEAQHATDSEVRARQEVEKSYKTIEVQFSELQTKADEQSRQLQDFAALKNRLNNENGDLNRTLEEMDNQVNSLHRLKSTLQSQLDETRRNFEEESRERQALAATAKNLEHENEILREHLDEEAESKADLTRQISKLNAEIQQWKARFDSEGLNKLEEIEAAKKALQLKVQELSDTNEGLFAKIASQEKVRHKLMQDLDDAQSDVEKAAAQVAYYEKHRRQFEKIVEEWKKKTDDLASELDAAQRDNRQLSTDLFKAKTANDELAEYLDSTRRENKSLAQEVKDLTDQLGEGGRSVAELQKIVRRLEVEKEELQKALDEAEAALEAEEAKVLRAQIEVSQIRSEIEKRIQEKEEEFENTRRNHQRALESMQATLEAETKQKEEALRIKKKLESDINDLEIALDHANRANADAQKTIKKYMETVRELQVQIEEEQRQKDELREQFLASEKRNGILQAEKDELAQQAEAAERARRNAEADCIELREQNNDLSNQVSSLTGWRRKLEGELLAVHAELEELVTELKNAQEQGQKASADAARLAEELRQEQEHSMHIERIRKGLELQIKEMQIRLDDAENAALKGGKKIIAQLEARIRAIEQELDGEQRRHQDTEKNWRKAERRVKEVEFQVIEEKKNEERLTELVDKLQTKLKIFKRQVEEAEEVAASNLNKYKVLQAQFEQADERAEIAENALSKMRNKIRASA 1941
BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Match: gi|75056481|sp|Q9TV62.1|MYH4_PIG (RecName: Full=Myosin-4; AltName: Full=Myosin heavy chain 2b; Short=MyHC-2b; AltName: Full=Myosin heavy chain 4; AltName: Full=Myosin heavy chain, skeletal muscle, fetal) HSP 1 Score: 1596.25 bits (4132), Expect = 0.000e+0 Identity = 865/1919 (45.08%), Postives = 1245/1919 (64.88%), Query Frame = 0
Query: 72 PYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLI-GHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKSK-----KASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKA-STKSDKNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYEDA----------------------------ANHLNELSQARQEIE---------------------------------------RRLAEKEEEFDAVRRSHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGR 1915
PYL S + + + KP+D K S +V + + FV+G VQS +G K V T K +QV +NPPKF+K EDM+ +T+L+E +VL+NLK RY A +IYTYSGLFCV VNPYK P+Y V Y GK+R E PPH+F+ISD AY+ ML++ NQS+LITGESGAGKT NTK+VI YFA + EKK++ + +LEDQI+ NP+LEAFGNAKT RNDNSSRFGKFIRIHF +GKLA DIETYLLEKSR+TFQ + ERSYHIFYQ+M + P++ ++ ++ + YDY +VSQG+ +VPSIDD+E+L T A IL FT +E V+IYK AVMH G +KFKQK REEQ EPD E A K + G++ ++KA C P++KVG E+VTKGQ + Q AV +A+ +YD++F ++V + NQ L D + FIGVLDIAGFEIF +N EQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDL ACI + EKPMGI +ILEEE +FPKATD SF++KL HLGKS NF K K AHF+++HYAGTV YN++GWL+KNKDPLN+TVV L + S + +FA+ E TKKG K KGS +TVS ++ L+ LM+ L +T PHF+RCI+PN K PG ++ LVLHQL CNGVLEGIRIC +GFP+R+ Y +F RY +L+A+ I + D KK +E + ID +++ G+TKVFF+AG+LG LEE+RD+ + +L+ Q C GFL R +F K ++RE I IQ N R F+++++W W + K KPL+ E+E+ +++ K E +R+ LE++ L ++ + +++ E L +ER + K E ++ + ++ E E + N L +KR LE E ++KD+ +LE L K E EK ++K++NL +++A LDE ++KL KEKK +QE + + +DL + EDKVN L K+KLEQ +DDLE SLE+EK+ + D+++ +RK+EGDLK+ QE +++E K++L+ ++++E ++ + +++E EQ + Q+ IKE QAR EELEEE+EAER +RAKAE+Q+ DL REL+E+SERLEEA GAT+AQ+E+NKKRE E ++R+DLEEAT+Q E+T +L+KKH D++ E+ EQ+D L ++K K+E +K+ ++ E ++L +M+ + ++K + EK + + L E+ + E + ++ + E+GE RQL+E + +S L++ K T Q+E+ KR E+E K + +L ++ H+ D + YEEE AK +L R KA E WR YE + I + EELE +K KL RL + E VE N K L+K K LQ E++ + V+ +N + ++KK R FDKI+ +WKHK + EL+ SQKE R+++TELF+VKN YE++ H++EL + +++IE R++AEK+EE D ++R+H + +E MQS L+AE++++ +A R+KKK+E D+ E+E L HAN E RN+ Q ++D+QL DD + + +E + ERRA+ +Q +EE R LEQ +R+RR AEQEL + +E + L QN SL K++LE ++ ++ +++++ E E+KAKKA+ DAA ++EEL+ EQD + HLE +K ++ VKDLQ RLD+AE A+K G+K QK+E+R++ELE+E++ EQ+R +++K L+K+ER++KE+ +Q EEDRK +QDLV+KLQ K++ +K+Q EEAEE + +NL+KFRK Q E EEAEERAD++E ++K RV R
Sbjct: 15 PYLRKSEKERIEAQNKPFDAKTSVFVAEPKESFVKGTVQSREGGKVTVKTEAGATLTVKEDQVFPMNPPKFDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPTPGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMS--NKKPELIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQGLNSADLLKALCYPRVKVGNEFVTKGQTVQQVYNAVGALAKAVYDKMFLWMVTRINQQL-DTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNETVVGLYQKSSVKTLAFLFAERQSSE---EGGTKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQAMCRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEDLAKSEAKRKELEEKMVALMQEKNDLQLQVQAEADGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDENIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALAMQLQKKIKELQARTEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMETVSKAKGNLEKMCRTLEDQLSEVKTKEEEHQRLINELSAQKARLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEELKRQLEEETKAKSALAHAVQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERSNAACAALDKKQRNFDKILAEWKHKYEETQAELEASQKESRSLSTELFKVKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQIEQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQMKRNHIRVVESMQSTLDAEIRSRNDALRIKKKMEGDLNEMEIQLNHANRQATEAIRNLRNTQGVLKDTQLHLDDAIRGQDDLKEQLAMVERRANLMQAEIEELRASLEQTERSRRVAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELENEVENEQKRNVEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQSKVKAYKRQAEEAEEQSNVNLSKFRKLQHELEEAEERADIAESQVNKLRVKSR 1927
BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Match: gi|75055810|sp|Q9BE39.1|MYH7_BOVIN (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1592.02 bits (4121), Expect = 0.000e+0 Identity = 869/1921 (45.24%), Postives = 1257/1921 (65.43%), Query Frame = 0
Query: 72 PYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLIGHEKK-TFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFA---NVGAREEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKA-STKSDKNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPG-QSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYEDAANHL----------------------------NELSQARQEIERRLAEKEE------------------------EFDAV-----RRSHQKGIEMMQ-------------SNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGR 1915
PYL S + + + +P+D KK +VPDD+ FV+ + S +G K H K T K +QV Q NPPKF+K EDM+ LT+L+E +VL+NLK RY + +IYTYSGLFCV +NPYK P+Y V Y GK+R+E PPH+F+ISD AY+ ML++ NQS+LITGESGAGKT NTK+VI YFA +G R +K++ + K +LEDQI+Q NP LEAFGNAKT RNDNSSRFGKFIRIHF A+GKLA DIETYLLEKSR+ FQ + ER YHIFYQ++ + P++ + ++ + YDY ++SQG+T+V SIDD E+L T AF++L FT EE ++YK A+MH G MKFK K REEQ EPD E+A K ++G++ ++K C P++KVG E+VTKGQN+ Q A +A+ +Y+R+F ++V + N TL + + FIGVLDIAGFEIF +N FEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDLQACI + EKPMGI++ILEEE +FPKATD +F+ KL NHLGKS NF K + K AHF+++HYAGTV YN+ GWL+KNKDPLN+TVV+L K S ++ +FA++ G +P+ + KGK KGS +TVS ++ L+ LM+ L +T PHF+RCI+PN K PG ID+ LV+HQL CNGVLEGIRIC +GFPNR+ Y +F RY IL+ I + D +K E + ID +++ G+TKVFF+AG+LG LEE+RD+ + +++ +Q G L R +F+K ++R+ + +IQ N R F+ ++NW W + K KPL+ E+EI ++++ + A E R+ LE++ L ++ + +++ EQ +L +ER + K E ++ + ++LE E + N L +KR LE E +++D+ +LE L K E EK ++K++NL +++A LDEI++KL KEKK +QE + +A +DL + EDKVN L K KLEQ +DDLE SLE+EK+ + D+++ +RK+EGDLK+ QE ++++E K++L+ +++++ ++ +NAR+E EQ ++ Q+ +KE QAR+EELEEELEAER ARAK E+ + DL REL+E+SERLEEA GAT+ Q+E+NKKRE E ++R+DLEEAT+Q E+T +L+KKH D++ E+SEQ+D L ++K K+E +K+ + E +++ +M+ ++++KA+ EK + + + E + E + D+ + K ENGE+ RQL+E + IS L + K+ T QLED KR E+E K + +L ++ H+ D + YEEE AK +L R KA E WR YE + I + EELE +K KL RL + E VE N K L+K K LQ EI+ + V+ +N + ++KK R FDKI+ +WK K + EL+ SQKE R+++TELF++KN YE++ HL +EL + R+++E AEK E EF+ + R+ +K EM Q ++L+AE +++ EA R+KKK+E D+ E+E L HAN E Q+ + Q+ ++D+Q++ DD + +EN+ ERR + LQ LEE R ++EQ +R+R+ AEQEL E +E + L QN SL K+++E ++ L+ +++E E E+KAKKA+ DAA ++EEL+ EQD + HLE +K ++ +KDLQ RLD+AE A+K G+K QK+E+R++ELE+EL+ EQ+R A+S+K ++K+ER+IKE+ +Q EEDRK +QDLV+KLQ+K++ +K+Q EEAEE A NL+KFRK Q E +EAEERAD++E ++K R R
Sbjct: 14 PYLRKSEKERLEAQTRPFDLKKDVFVPDDKEEFVKATILSREGGKVTAETEHGKTVTVKEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTINPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQATGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILS--NKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTTEEKNSMYKLTGAIMHFGNMKFKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVVYAKGALAKAVYERMFNWMVTRINATL-ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSSNFQKPRNIKGKPEAHFSLIHYAGTVDYNIIGWLQKNKDPLNETVVDLYKKSSLKMLSSLFANYAGFDTPI---EKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEIALMKEEFGRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELSEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLE---AEKLELQSALEEAEASLEQEEGKILRAQLEFNQIKAEMERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRLAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMEADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSR 1925
BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Match: gi|586830498|sp|P02566.2|MYO4_CAEEL (RecName: Full=Myosin-4; AltName: Full=Myosin heavy chain B; Short=MHC B; AltName: Full=Uncoordinated protein 54) HSP 1 Score: 1591.63 bits (4120), Expect = 0.000e+0 Identity = 900/1945 (46.27%), Postives = 1255/1945 (64.52%), Query Frame = 0
Query: 64 NEPDPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLIGHEKK-TFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGARE-----EKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSDKN--AHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLK-CGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKG---SGA-KTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDFID-----KERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYE---------------------DAANHLNELSQA---RQEIERRL-AEKEE------------------------EFDAVRRSHQKGIEMMQSNLEAEMKAKAEA-QRMKKKLE-----------------ADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRAT-TPNPG 1922
+E DP YL + E +D KPYD KK+ W+PD + G++ G + + G + ++ + T K E V ++NPPKFEK EDMSNL++LN+ASVL NL++RY A LIYTYSGLFCV +NPYKR PIYT + ++++GKR+ E+PPHLFA+SD AYRNML + NQSMLITGESGAGKTENTKKVI YFA VGA + E KK +LEDQIVQTNP+LEAFGNAKT RN+NSSRFGKFIRIHFN G+LA CDIE YLLEKSR+ Q ER YHIFYQ+ D P++KK L I DY +V+Q + + IDD E+ + T EAF+IL+F+ E + Y+ ++A MHMG MKFKQ+ REEQ EPD ++A+K ++ G+ E +KA KP++KVGTEWV+KGQN +Q AV +A+GLY RVF ++V+KCN TL + + FIGVLDIAGFEIF +N FEQ+ INF NEKLQQFFNHHMFVLEQEEY EGI W +DFG+DLQACI + EKP+GI+++L+EE + PKATD + KL HLGK NF K K AHFA+ HYAGTV YN WLEKNKDPLNDTVV +K N+L+V I+ D+ Q + G K SG+ TVS Y+ L++LM+ L+ T PHFIRCI+PN K G ID+ LVL+QLTCNGVLEGIRIC +GFPNR +P+F RYAIL A + D KK E I + ++ +E FRIG TKVFF+AGVL +LE++RD+ + ++ Q L KD ++R +QR + ++QRN R + +LR W WF + K KP++ EE++ + D ++ EE R+ LE+ + +L E+ ++ +E+ + L +ER AK Q+ +LS+ D+L R +Q K+ +E+EV ++K + +LE L KAESEK ++DH++R+L D++ DE ++KLNKEKK+ +E N K EDL S EDK NH N VK+KLEQT+DDLEDSLEREKR + D+DKQ+RKVEG+LK+ QE + E R + +LE+ ++++E ++ +++RLE EQ +K QR IK+ Q+R+ ELEEELE ERQ+R+KA+R K DL REL+EL E+L+E GAT AQ+E+NKKRE EL +LR+DLEEA + E+ + L+KKH DA+ E+++Q+DQL K KAKVE DK R+ E+L +D K + EK K F+ L EL + E S QL D + + +ENG+++RQLE+ + ++ L + K QLT+QLE+A+R A++EA+ERQ++ + +N +HE + + EEE+ K+++ RQ KA + W+ +E EG+ K +ELE++K + ++ E + ++ N+K L+K K+ L ++D V+ AN S +EKK + FDKII +W+ K D L ELD +Q++ RN +T+LF+ KN E D + L E ++ Q+I RRL EKEE E +R +K I+ + E K A A + M+ LE D+ ELE AL+HAN + + Q+N+ +YQ Q+R+ QL+ ++EQ+ A RE NAE+RA LQ+ EE E A+RAR+ AE E ++ + ++ + Q SL + KR+LEGE+ + DLDE E E+++KKA+ DA R++EELR EQ+ +QH++ RK L+ Q+K++Q+RLD+AE A+K G+K K+E R++ELESELDGEQRRF D+ KNL + +R+++E+ FQ +ED+K E +QDL++KLQ KL+ KKQ+EEAEE+A +NL K+++ + E+AEERAD +E +LSK R RA+ + PG
Sbjct: 3 HEKDP-GWQYLRRTREQVLEDQSKPYDSKKNVWIPDPEEGYLAGEITATKGDQVTIVTARGNEVTLKKELVQEMNPPKFEKTEDMSNLSFLNDASVLHNLRSRYAAMLIYTYSGLFCVVINPYKRLPIYTDSCARMFMGKRKTEMPPHLFAVSDEAYRNMLQDHENQSMLITGESGAGKTENTKKVICYFAAVGASQQEGGAEVDPNKKKVTLEDQIVQTNPVLEAFGNAKTVRNNNSSRFGKFIRIHFNKHGRLASCDIEHYLLEKSRVIRQAPGERCYHIFYQIYS--DFRPELKKELLLDLPIKDYWFVAQAELIIDGIDDVEEFQLTDEAFDILNFSAVEKQDCYRLMSAHMHMGNMKFKQRPREEQAEPDGTDEAEKASNMYGIGCEEFLKALTKPRVKVGTEWVSKGQNCEQVNWAVGAMAKGLYSRVFNWLVKKCNLTLDQKGIDRDYFIGVLDIAGFEIFDFNSFEQLWINFVNEKLQQFFNHHMFVLEQEEYAREGIQWVFIDFGLDLQACIELIEKPLGIISMLDEECIVPKATDLTLASKLVDQHLGKHPNFEKPKPPKGKQGEAHFAMRHYAGTVRYNCLNWLEKNKDPLNDTVVSAMKQSKGNDLLVEIWQDYTTQEEAAAKAKEGGGGGKKKGKSGSFMTVSMLYRESLNNLMTMLNKTHPHFIRCIIPNEKKQSGMIDAALVLNQLTCNGVLEGIRICRKGFPNRTLHPDFVQRYAILAAKEAK--SDDDKKKCAEAIMSKLVNDGSLSEEMFRIGLTKVFFKAGVLAHLEDIRDEKLATILTGFQSQIRWHLGLKDRKRRMEQRAGLLIVQRNVRSWCTLRTWEWFKLYGKVKPMLKAGKEAEELEKINDKVKALEDSLAKEEKLRKELEESSAKLVEEKTSLFTNLESTKTQLSDAEERLAKLEAQQKDASKQLSELNDQLADNEDRTADVQRAKKKIEAEVEALKKQIQDLEMSLRKAESEKQSKDHQIRSLQDEMQQQDEAIAKLNKEKKHQEEINRKLMEDLQSEEDKGNHQNKVKAKLEQTLDDLEDSLEREKRARADLDKQKRKVEGELKIAQENIDESGRQRHDLENNLKKKESELHSVSSRLEDEQALVSKLQRQIKDGQSRISELEEELENERQSRSKADRAKSDLQRELEELGEKLDEQGGATAAQVEVNKKREAELAKLRRDLEEANMNHENQLGGLRKKHTDAVAELTDQLDQLNKAKAKVEKDKAQAVRDAEDLAAQLDQETSGKLNNEKLAKQFELQLTELQSKADEQSRQLQDFTSLKGRLHSENGDLVRQLEDAESQVNQLTRLKSQLTSQLEEARRTADEEARERQTVAAQAKNYQHEAEQLQESLEEEIEGKNEILRQLSKANADIQQWKARFEGEGLLKADELEDAKRRQAQKINELQEALDAANSKNASLEKTKSRLVGDLDDAQVDVERANGVASALEKKQKGFDKIIDEWRKKTDDLAAELDGAQRDLRNTSTDLFKAKNAQEELAEVVEGLRRENKSLSQEIKDLTDQLGEGGRSVHEMQKIIRRLEIEKEELQHALDEAEAALEAEESKVLRAQVEVSQIRSEIEKRIQEKEEEFENTRKNHARALESMQASLETEAKGKAELLRIKKKLEGDINELEIALDHANKANADAQKNLKRYQEQVRELQLQVEEEQRNGADTREQFFNAEKRATLLQSEKEELLVANEAAERARKQAEYEAADARDQANEANAQVSSLTSAKRKLEGEIQAIHADLDETLNEYKAAEERSKKAIADATRLAEELRQEQEHSQHVDRLRKGLEQQLKEIQVRLDEAEAAALKGGKKVIAKLEQRVRELESELDGEQRRFQDANKNLGRADRRVRELQFQVDEDKKNFERLQDLIDKLQQKLKTQKKQVEEAEELANLNLQKYKQLTHQLEDAEERADQAENSLSKMRSKSRASASVAPG 1942
BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Match: gi|125987844|sp|P79293.2|MYH7_PIG (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1590.09 bits (4116), Expect = 0.000e+0 Identity = 869/1921 (45.24%), Postives = 1256/1921 (65.38%), Query Frame = 0
Query: 72 PYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLIGHEKK-TFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFA---NVGAREEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKA-STKSDKNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPG-QSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYEDAANHL----------------------------NELSQARQEIERRLAEKEE------------------------EFDAV-----RRSHQKGIEMMQ-------------SNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGR 1915
PYL S + + + +P+D KK +VPDD+ FV+ + S +G K H K T K +QV Q NPPKF+K EDM+ LT+L+E +VL+NLK RY + +IYTYSGLFCV +NPYK P+Y V Y GK+R+E PPH+F+ISD AY+ ML++ NQS+LITGESGAGKT NTK+VI YFA +G R +K++ K +LEDQI+Q NP LEAFGNAKT RNDNSSRFGKFIRIHF A+GKLA DIETYLLEKSR+ FQ + ER YHIFYQ++ + P++ + ++ + YDY ++SQG+T+V SIDD E+L T AF++L FT EE ++YK A+MH G MKFK K REEQ EPD E+A K ++G++ ++K C P++KVG E+VTKGQN+ Q A +A+ +Y+++F ++V + N TL + + FIGVLDIAGFEIF +N FEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDLQACI + EKPMGI++ILEEE +FPKATD +F+ KL NHLGKS NF K + K AHFA++HYAGTV YN+ GWL+KNKDPLN+TVV+L K S L+ ++FA++ G +P+ + KGK KGS +TVS ++ L+ LM+ L +T PHF+RCI+PN K PG ID+ LV+HQL CNGVLEGIRIC +GFPNR+ Y +F RY IL+ I + D +K E + ID +++ G+TKVFF+AG+LG LEE+RD+ + +++ +Q G L R +F+K ++R+ + +IQ N R F+S++NW W + K KPL+ E+E+ +++ + A E R+ LE++ L ++ + +++ EQ +L +ER + K E ++ + ++LE E + N L +KR LE E +++D+ +LE L K E EK ++K++NL +++A LDEI++KL KEKK +QE + +A +DL + EDKVN L K KLEQ +DDLE SLE+EK+ + D+++ +RK+EGDLK+ QE ++++E K++L+ +++++ ++ +NAR+E EQ ++ Q+ +KE QAR+EELEEELEAER ARAK E+ + DL REL+E+SERLEEA GAT+ Q+E+NKKRE E ++R+DLEEAT+Q E+T +L+KKH D++ E+ EQ+D L ++K K+E +K+ + E +++ +M+ ++++KA+ EK + + + E + E + D+ + K ENGE+ RQL+E + IS L + K+ T QLED KR E+E K + +L ++ H+ D + YEEE AK +L R KA E WR YE + I + EELE +K KL RL + E VE N K L+K K LQ EI+ + V+ +N + ++KK R FDKI+ +WK K + EL+ SQKE R+++TELF++KN YE++ HL +EL + R+++E AEK E EF+ + R+ +K EM Q ++L+AE +++ EA R+KKK+E D+ E+E L HAN E Q+ + Q+ ++D+Q++ DD + +EN+ ERR + LQ LEE R ++EQ +R+R+ AEQEL E +E + L QN SL K+++E ++ L+ +++E E E+KAKKA+ DAA ++EEL+ EQD + HLE +K ++ +KDLQ RLD+AE A+K G+K QK+E+R++ELE+EL+ EQ+R A+S+K ++K+ER+IKE+ +Q EEDRK +QDLV+KLQ+K++ +K+Q EEAEE A NL+KFRK Q E +EAEERAD++E ++K R R
Sbjct: 14 PYLRKSEKERLEAQTRPFDLKKDVYVPDDKEEFVKAKILSREGGKVTAETEHGKTVTVKEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTINPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILS--NKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMKFKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVMYATGALAKAVYEKMFNWMVTRINTTL-ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGRPEAHFALIHYAGTVDYNIIGWLQKNKDPLNETVVDLYKKSSLKLLSNLFANYAGADTPV---EKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMSVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLE---AEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEMERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMEADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSR 1925
BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Match: gi|75054114|sp|Q8MJU9.1|MYH7_HORSE (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1588.16 bits (4111), Expect = 0.000e+0 Identity = 869/1921 (45.24%), Postives = 1259/1921 (65.54%), Query Frame = 0
Query: 72 PYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLIGHEKK-TFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFA---NVGAREEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKA-STKSDKNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPG-QSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYEDAANHL----------------------------NELSQARQEIERRLAEKEE------------------------EFDAV-----RRSHQKGIEMMQ-------------SNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGR 1915
PYL + + + +D +P+D KK +VPDD+ FV+ + S +G K H K T K +QV Q NPPKF+K EDM+ LT+L+E +VL+NLK RY A +IYTYSGLFCV +NPYK P+YT V Y GK+R+E PPH+F+ISD AY+ ML++ NQS+LITGESGAGKT NTK+VI YFA +G R +K + S K +LEDQI++ NP LEAFGNAKT RNDNSSRFGKFIRIHF A+GKLA DIETYLLEKSR+ FQ + ER YHIFYQ++ + P++ + ++ + YDY ++SQG+T+V SIDD E+L T AF++L FT EE ++YK A+MH G MKFKQK REEQ EPD E+A K ++G++ ++K C P++KVG E+VTKGQN+ Q A +A+ +Y+R+F ++V + N TL + + FIGVLDIAGFEIF +N FEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDLQACI + EKPMGI++ILEEE +FPKATD +F+ KL NHLGKS NF K + K AHF+++HYAGTV YN+ GWL+KNKDPLN+TVV+L K S ++ ++FA++ G +P+ + KGK KGS +TVS ++ L+ LM+ L +T PHF+RCI+PN K PG ID+ LV+HQL CNGVLEGIRIC +GFPNR+ Y +F RY IL+ I + D +K E + + ID ++R G+TKVFF+AG+LG LEE+RD+ + +++ +Q G L R +F+K ++R+ + +IQ N R F+ ++NW W + K KPL+ E+E+ +++ + A E R+ LE++ L ++ + +++ EQ +L +ER + K E ++ + ++LE E + N L +KR LE E +++D+ +LE L K E EK ++K++NL +++A LDEI++KL KEKK +QE + +A +DL + EDKVN L K KLEQ +DDLE SLE+EK+ + D+++ +RK+EGDLK+ QE ++++E K++L+ +++++ ++ +NAR+E EQ ++ Q+ +KE QAR+EELEEELEAER ARAK E+ + DL REL+E+SERLEEA GAT+ Q+E+NKKRE E ++++DLEEAT+Q E+T +L+KKH D++ E+ EQ+D L ++K K+E +K+ + E +++ +M+ ++++KA+ EK + + + E + E + D+ + K ENGE+ RQL+E + IS L + K+ T QLED KR E+E K + +L ++ H+ D + YEEE AK +L R KA E WR YE + I + EELE +K KL RL + E VE N K L+K K LQ EI+ + V+ +N + ++KK R FDKI+ +WK K + EL+ SQKE R+++TELF++KN YE++ HL +EL + R+++E AEK E EF+ + R+ +K EM Q ++L+AE +++ EA R+KKK+E D+ E+E L HAN E Q+ + Q+ ++D+Q++ DD + +EN+ ERR + LQ LEE R ++EQ +R+R+ AEQEL E +E + L QN SL K++++ ++ L+ +++E E E+KAKKA+ DAA ++EEL+ EQD + HLE +K ++ +KDLQ RLD+AE A+K G+K QK+E+R++ELE+EL+ EQ+R A+S+K ++K+ER+IKE+ +Q EEDRK +QDLV+KLQ+K++ +K+Q EEAEE A NL+KFRK Q E +EAEERAD++E ++K R R
Sbjct: 14 PYLRKTEKERLEDQTRPFDLKKDVFVPDDKEEFVKAKIISREGGKITAETEHGKTVTVKEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYAAWMIYTYSGLFCVTINPYKWLPVYTAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTSGKGTLEDQIIEANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILS--NKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMKFKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYAKGALAKAVYERMFNWMVARINATL-ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSSNFQKPRNIKGKPEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVDLYKKSSLKMLSNLFANYLGADAPI---EKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYRFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFARLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMKRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLE---AEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRDAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEVEQKRNAESIKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSR 1925
BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Match: gi|125987843|sp|P49824.3|MYH7_CANFA (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1582.77 bits (4097), Expect = 0.000e+0 Identity = 864/1920 (45.00%), Postives = 1253/1920 (65.26%), Query Frame = 0
Query: 72 PYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLIGHEKK-TFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFA---NVGAREEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKA-STKSDKNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYEDAANHL----------------------------NELSQARQEIERRLAEKEE------------------------EFDAV-----RRSHQKGIEMMQ-------------SNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGR 1915
P+L S + + + +P+D KK +VPDD+ FV+ + S +G K + K T K +QV Q NPPKF+K EDM+ LT+L+E +VL+NLK RY + +IYTYSGLFCV VNPYK P+Y V Y GK+R+E PPH+F+ISD AY+ ML++ NQS+LITGESGAGKT NTK+VI YFA +G R +K + K +LEDQI+Q NP LEAFGNAKT RNDNSSRFGKFIRIHF A+GKLA DIETYLLEKSR+ FQ + ER YHIFYQ++ + P++ + ++ + YDY ++SQG+T+V SIDD E+L T AF++L FT EE ++YK A+MH G MKFKQK REEQ EPD E+A K ++G++ ++K C P++KVG E+VTKGQN+ Q A +A+ +Y+++F ++V + N TL + + FIGVLDIAGFEIF +N FEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDLQACI + EKPMGI++ILEEE +FPKATD +F+ KL NHLGKS NF K + K + AHF+++HYAGTV YN+ GWL+KNKDPLN+TVV L + S L+ ++FA++ G E KGK KGS +TVS ++ L+ LM+ L +T PHF+RCI+PN K PG ID+ LV+HQL CNGVLEGIRIC +GFPNR+ Y +F RY IL+ I + D +K E + + ID +++ G+TKVFF+AG+LG LEE+RD+ + +++ +Q G L R +++K ++R+ + +IQ N R F+ ++NW W + K KPL+ E+E+ +++ + A E R+ LE++ L ++ + +++ EQ +L +ER + K E ++ + ++LE E + N L +KR LE E +++D+ +LE L K E EK ++K++NL +++A LDEI++KL KEKK +QE + +A +DL + EDKVN L K KLEQ +DDLE SLE+EK+ + D+++ +RK+EGDLK+ QE ++++E K++L+ +++++ ++ +NAR+E EQ ++ Q+ +KE QAR+EELEEELEAER ARAK E+ + DL REL+E+SERLEEA GAT+ Q+E+NKKRE E ++R+DLEEAT+Q E+T +L+KKH D++ E+ EQ+D L ++K K+E +K+ + E +++ +M+ ++++KA+ EK + + + E + E + D+ + K ENGE+ RQL+E + IS L + K+ T QLED KR E+E K + +L ++ H+ D + YEEE AK +L R KA E WR YE + I + EELE +K KL RL + E VE N K L+K K LQ EI+ + V+ +N + ++KK R FDKI+ +WK K + EL+ SQKE R+++TELF++KN YE++ HL +EL + R+++E AEK E EF+ + R+ +K EM Q ++L+AE +++ EA R+KKK+E D+ E+E L HAN E Q+ + Q+ ++D+Q++ DD + +EN+ ERR + LQ LEE R ++EQ +R+R+ AEQEL E +E + L QN SL K++++ ++ L+ +++E E E+KAKKA+ DAA ++EEL+ EQD + HLE +K ++ +KDLQ RLD+AE A+K G+K QK+E+R++ELE+EL+ EQ+R A+S+K ++K+ER+IKE+ +Q EEDRK +QDLV+KLQ+K++ +K+Q EEAEE A NL+KFRK Q E +EAEERAD++E ++K R R
Sbjct: 14 PFLRKSEKERLEAQTRPFDLKKDVFVPDDKEEFVKAKIVSREGGKVTAETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILS--NKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMKFKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYATGALAKAVYEKMFNWMVTRINATL-ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVALYQKSSLKLLSNLFANYAGADAPVE--KGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEYKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFARIKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLE---AEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKGLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSR 1925
BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Match: gi|83304912|sp|P12883.5|MYH7_HUMAN (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1580.84 bits (4092), Expect = 0.000e+0 Identity = 866/1921 (45.08%), Postives = 1253/1921 (65.23%), Query Frame = 0
Query: 72 PYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLIGHEKK-TFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFA---NVGAREEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKA-STKSDKNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPG-QSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYEDAANHL----------------------------NELSQARQEIERRLAEKEE------------------------EFDAV-----RRSHQKGIEMMQ-------------SNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGR 1915
PYL S + + + +P+D KK +VPDD+ FV+ + S +G K + K T K +QV Q NPPKF+K EDM+ LT+L+E +VL+NLK RY + +IYTYSGLFCV VNPYK P+YT V Y GK+R+E PPH+F+ISD AY+ ML++ NQS+LITGESGAGKT NTK+VI YFA +G R +K + K +LEDQI+Q NP LEAFGNAKT RNDNSSRFGKFIRIHF A+GKLA DIETYLLEKSR+ FQ + ER YHIFYQ++ + P++ + ++ + YDY ++SQG+T+V SIDD E+L T AF++L FT EE ++YK A+MH G MKFK K REEQ EPD E+A K ++G++ ++K C P++KVG E+VTKGQN+ Q A +A+ +Y+R+F ++V + N TL + + FIGVLDIAGFEIF +N FEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDLQACI + EKPMGI++ILEEE +FPKATD +F+ KL NHLGKS NF K + K AHF+++HYAG V YN+ GWL+KNKDPLN+TVV L + S L+ +FA++ G +P+ + KGK KGS +TVS ++ L+ LM+ L +T PHF+RCI+PN K PG +D+ LV+HQL CNGVLEGIRIC +GFPNR+ Y +F RY IL+ I + D +K E + + ID +++ G+TKVFF+AG+LG LEE+RD+ + +++ +Q G L R +++K ++R+ + VIQ N R F+ ++NW W + K KPL+ E+E+ +++ + A E R+ LE++ L ++ + +++ EQ +L +ER + K E ++ + ++LE E + N L +KR LE E +++D+ +LE L K E EK ++K++NL +++A LDEI++KL KEKK +QE + +A +DL + EDKVN L K KLEQ +DDLE SLE+EK+ + D+++ +RK+EGDLK+ QE ++++E K++L+ +++++ ++ +NAR+E EQ ++ Q+ +KE QAR+EELEEELEAER ARAK E+ + DL REL+E+SERLEEA GAT+ Q+E+NKKRE E ++R+DLEEAT+Q E+T +L+KKH D++ E+ EQ+D L ++K K+E +K+ + E +++ +M+ ++++KA+ EK + + + E + E + D+ + K ENGE+ RQL+E + IS L + K+ T QLED KR E+E K + +L ++ H+ D + YEEE AK +L R KA E WR YE + I + EELE +K KL RL E E VE N K L+K K LQ EI+ + V+ +N + ++KK R FDKI+ +WK K + EL+ SQKE R+++TELF++KN YE++ HL +EL + R+++E AEK E EF+ + R+ +K EM Q ++L+AE +++ EA R+KKK+E D+ E+E L HAN E Q+ + Q+ ++D+Q++ DD + +EN+ ERR + LQ LEE R ++EQ +R+R+ AEQEL E +E + L QN SL K++++ ++ L+ +++E E E+KAKKA+ DAA ++EEL+ EQD + HLE +K ++ +KDLQ RLD+AE A+K G+K QK+E+R++ELE+EL+ EQ+R A+S+K ++K+ER+IKE+ +Q EEDRK +QDLV+KLQ+K++ +K+Q EEAEE A NL+KFRK Q E +EAEERAD++E ++K R R
Sbjct: 14 PYLRKSEKERLEAQTRPFDLKKDVFVPDDKQEFVKAKIVSREGGKVTAETEYGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYGSWMIYTYSGLFCVTVNPYKWLPVYTPEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILS--NKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMKFKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVIYATGALAKAVYERMFNWMVTRINATL-ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSANFQKPRNIKGKPEAHFSLIHYAGIVDYNIIGWLQKNKDPLNETVVGLYQKSSLKLLSTLFANYAGADAPI---EKGKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAEREKEMASMKEEFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLE---AEKMELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSR 1925
BLAST of low quality protein: myosin heavy muscle vs. nr
Match: gi|1325254663|ref|XP_023341687.1| (myosin heavy chain, muscle-like isoform X1 [Eurytemora affinis]) HSP 1 Score: 2333.52 bits (6046), Expect = 0.000e+0 Identity = 1203/1948 (61.76%), Postives = 1494/1948 (76.69%), Query Frame = 0
Query: 54 MPGNVKLGSSNEPDPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSDKNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDFIDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYEDAANHLNELSQARQEIERRLAEKEEEFDAVRRSHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVT-HQENQRN------IDKYQNQIRDS----------------------QLRFDDEQKVKA-------------------------IARENMLNAERRAHTLQNA---LEE---------------------TRTLLEQA----DRARRA---AEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRA 1916
MPGN K+GS DPDP PYL V+ ++KR DM KPYDPKKSYWVPD +GGF+EGL++S++G K +V+I HEK+T K EQ+ QVNPPKFEKCEDMSNLTYLNEASVL NLKARYQA+LIYTYSGLFCVA+NPYKR PIYT+ TVKLYLGKRR EVPPHLFAISD AYRNML G QS+L+TGESGAGKTENTKKVISYFA VGAR+ K S K SLEDQIVQTNPILE+FGNAKTARNDNSSRFGKFIRIHFN+SGKLAGCDIETYLLEK+RITFQQEVERSYHIFYQMM+ + PD+KK C LS DIYDYH+VS GKT VPSIDD EDLEFTHEAF IL F EE T N+Y++ AAVMHMGE+KFKQKGREEQCEPD+ QAKKV +LG++PE+MMKA+CKPKIKVGTEWVTKGQN+DQS +VAGIARGLY+RVF F+VEKCN+TLVD TMKKVVFIGVLDIAGFEIF++NGFEQ+CINFCNEKLQQFFNHHMFVLEQEEY+ EGIDW MVDFGMDLQ+CITMFEKP+GIL+ILEEESLFPKATDK+FEDKLK NHLGKS NF KA TK DKNAHFAIVHYAGTVSYN++ WLEKNKDPLNDTV+ELLK G N L+ H+F DHPGQ ED +KGKK KG GAKTVSTFY QL+SLM+TLHATEPHFIRCI+PN +K PG+I+ LVLHQLTCNGVLEGIRICMRGFPNRMPYPEF RY IL K S D K IC + ++KE++RIG K+FFRAGVLGYLEEVRD+ V +++R++QG C G LRR+DF KR++QR+L+ VIQ+ FR++L+LR+WGWFSIIQKTKPLIGM+NIEEEI ILE+AA KA + E E++RLE N +L E+ +A++KRIE EQGD++ QER AKAA QKA E +L + +K++ E R L +KR +E++V ++R D+ +LE + KAE E+ +DH++R+LNDDIA+ DE++SKLNKEKK+ E N KA E+L +A++KV+HL MVK+KLEQT+DDLED+LERE+R +N+ +KQRRK+EGD+K+ QE ++E+E+GK+EL+ + R+ERDI E+ +L EQ G +K + IKETQARVEE+EEELEAERQAR KAERQ+ +L RE++EL+ER+EEA GAT AQMELNKKRE E+ R+RKDLEE IQ E+TI SLKKKHQDAIGEMSEQ++QL KL+AKVE D + I+ E ++L+V D+++R+KA+ EKA K L +L+K+++E +LQ+ DM+ GN+KS +EN E+MRQL++VD N+SML+K + QL N+LE K+M ++E+KERQS LGR+RNL+HE+DG+ AVYEEE+ AK++ RQ +KAE+E +LWR YE +GIAKIEELEN+K+KLQARLAECEGTVEN N KL+QL+K+K LQ+ I+ + + VD AN+ QM+KK++QFDK++ DWK KAD +++EL++SQ++CRNV+TELFRVKNGYE+ L+E+ + + + + + E+ RS E ++ +K+LE + EL++ALE A T QE +N ID+ +N I Q +DE K KA + EN N R +Q A LEE + LE+A D+ RA AEQE+ EC+E +SDLS+QNQSL +R+LE E +NLRQD D+MK E E++A+ AM+DAA+++EELR+EQ+ T H E RK DA +K+LQ+++DD E+++MK G+K K+ESRIKELESELD EQRR D+ KNLKK ER+IKE++FQ EE+RKQ MQ+LV++LQ KLR FK+QIEEAEEIAA+NL K+RK QG+ EEAEERA L+EQA +K + RA
Sbjct: 1 MPGNEKIGSGTGVDPDPAPYLYVTFDMKRNDMSKPYDPKKSYWVPDGEGGFLEGLLESENGGKVVVMIQHEKRTLKKEQIQQVNPPKFEKCEDMSNLTYLNEASVLHNLKARYQAQLIYTYSGLFCVAINPYKRLPIYTNTTVKLYLGKRRTEVPPHLFAISDLAYRNMLDKGVCQSILVTGESGAGKTENTKKVISYFAMVGARDGPK-TSNKVSLEDQIVQTNPILESFGNAKTARNDNSSRFGKFIRIHFNSSGKLAGCDIETYLLEKARITFQQEVERSYHIFYQMMEP--AVPDLKKKCFLSNDIYDYHFVSMGKTKVPSIDDNEDLEFTHEAFRILCFGEENTYNVYRATAAVMHMGELKFKQKGREEQCEPDNPAQAKKVAALLGIEPESMMKAFCKPKIKVGTEWVTKGQNIDQSYHSVAGIARGLYERVFNFLVEKCNETLVDDTMKKVVFIGVLDIAGFEIFEFNGFEQLCINFCNEKLQQFFNHHMFVLEQEEYLKEGIDWEMVDFGMDLQSCITMFEKPLGILSILEEESLFPKATDKTFEDKLKANHLGKSPNFVKAITKHDKNAHFAIVHYAGTVSYNVTSWLEKNKDPLNDTVIELLKSGENKLINHVFRDHPGQGR-EEDGDQKGKKKKGGGAKTVSTFYTKQLNSLMTTLHATEPHFIRCIIPNTHKQPGQIEPALVLHQLTCNGVLEGIRICMRGFPNRMPYPEFIFRYGILQNGKP---NSSDEKVQAAAICTEMLNKEKYRIGMCKIFFRAGVLGYLEEVRDEKVTRMIRYMQGYCYGILRRRDFLKRKQQRDLLVVIQKLFRRYLTLRHWGWFSIIQKTKPLIGMLNIEEEINILEEAATKAVDEVQFEATEKKRLESANTRLMEEKMALLKRIEIEQGDIISLQERQAKAAAQKADLENQLDEMSEKIKAEENRKEELIKEKRAIEADVSNVRGDLSDLEVHVQKAEQERANKDHQLRSLNDDIASKDELISKLNKEKKHFLEINGKAVEELQNADEKVSHLTMVKNKLEQTLDDLEDNLERERRKRNEEEKQRRKIEGDVKLTQEAIIELEKGKKELDQCLARKERDIAELTGKLSEEQSGVSKLTKVIKETQARVEEMEEELEAERQARGKAERQRSELAREMEELTERIEEAGGATQAQMELNKKREAEVVRMRKDLEEINIQHEATIFSLKKKHQDAIGEMSEQIEQLNKLRAKVEKDNSNIKHEIDDLKVISDEVIRAKAAGEKAGKTLSTQLSDLNKKISEVNLQVNDMELGNRKSASENSELMRQLDDVDSNLSMLHKIRHQLNNELEQVKKMYDEESKERQSTLGRFRNLKHEFDGLAAVYEEEVTAKENFHRQCQKAEEETNLWRLKYEKDGIAKIEELENTKMKLQARLAECEGTVENLNTKLVQLEKSKNQLQENIEDITAKVDQANIINIQMDKKLKQFDKVVHDWKIKADNMSQELEYSQRDCRNVSTELFRVKNGYEECMGQLDEVKRENKMLSDDIRDIMEQISEGGRSIH------------------EIEKQRKRLETEKQELQAALEEAEATLEQEENKNARLQLEIDQVKNDIEKRLQEKEEEFEITKKNHHRMTEQIQETLEDESKAKAELMRCRKKLEADVSELEGALEQANHVHEENQRNIRRYQDLIQKANINLEEEQQAKDLARENHVNMDRRAHSLQNALEEARTILDQTDRARRSAEQEVGECHEQLSDLSMQNQSLAGARRKLEQEAENLRQDCDDMKGEARSCEERARNAMIDAAKLAEELRVEQEHTAHAEQARKMADALIKELQMKIDDVEVSSMKQGKKVCSKLESRIKELESELDAEQRRLGDNSKNLKKGERRIKEIEFQSEEERKQAGQMQELVDRLQNKLRAFKRQIEEAEEIAALNLAKYRKTQGDAEEAEERATLTEQAAAKLKSAARA 1923
BLAST of low quality protein: myosin heavy muscle vs. nr
Match: gi|1325254668|ref|XP_023341758.1| (myosin heavy chain, muscle-like isoform X2 [Eurytemora affinis]) HSP 1 Score: 2195.24 bits (5687), Expect = 0.000e+0 Identity = 1158/1954 (59.26%), Postives = 1452/1954 (74.31%), Query Frame = 0
Query: 54 MPGNVKLGSSNEPDPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSDKNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDFIDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYEDAANHLNELSQARQEIERRLAEKEEEFDAVRRSHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVT-HQENQRN------IDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRR-----------LEGE-------------MD----NLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAE----------MNAMKNGRKAAQKM-----------------ESRIKEL-----------------------------ESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRA 1916
MPGN K+GS DPDP PYL V+ ++KR DM KPYDPKKSYWVPD +GGF+EGL++S++G K +V+I HEK+T K EQ+ QVNPPKFEKCEDMSNLTYLNEASVL NLKARYQA+LIYTYSGLFCVA+NPYKR PIYT+ TVKLYLGKRR EVPPHLFAISD AYRNML G QS+L+TGESGAGKTENTKKVISYFA VGAR+ K S K SLEDQIVQTNPILE+FGNAKTARNDNSSRFGKFIRIHFN+SGKLAGCDIETYLLEK+RITFQQEVERSYHIFYQMM+ + PD+KK C LS DIYDYH+VS GKT VPSIDD EDLEFTHEAF IL F EE T N+Y++ AAVMHMGE+KFKQKGREEQCEPD+ QAKKV +LG++PE+MMKA+CKPKIKVGTEWVTKGQN+DQS +VAGIARGLY+RVF F+VEKCN+TLVD TMKKVVFIGVLDIAGFEIF++NGFEQ+CINFCNEKLQQFFNHHMFVLEQEEY+ EGIDW MVDFGMDLQ+CITMFEKP+GIL+ILEEESLFPKATDK+FEDKLK NHLGKS NF KA TK DKNAHFAIVHYAGTVSYN++ WLEKNKDPLNDTV+ELLK G N L+ H+F DHPGQ ED +KGKK KG GAKTVSTFY QL+SLM+TLHATEPHFIRCI+PN +K PG+I+ LVLHQLTCNGVLEGIRICMRGFPNRMPYPEF RY IL K S D K IC + ++KE++RIG K+FFRAGVLGYLEEVRD+ V +++R++QG C G LRR+DF KR++QR+L+ VIQ+ FR++L+LR+WGWFSIIQKTKPLIGM+NIEEEI ILE+AA KA + E E++RLE N +L E+ +A++KRIE EQGD++ QER AKAA QKA E +L + +K++ E R L +KR +E++V ++R D+ +LE + KAE E+ +DH++R+LNDDIA+ DE++SKLNKEKK+ E N KA E+L +A++KV+HL MVK+KLEQT+DDLED+LERE+R +N+ +KQRRK+EGD+K+ QE ++E+E+GK+EL+ + R+ERDI E+ +L EQ G +K + IKETQARVEE+EEELEAERQAR KAERQ+ +L RE++EL+ER+EEA GAT AQMELNKKRE E+ R+RKDLEE IQ E+TI SLKKKHQDAIGEMSEQ++QL KL+AK+E D I+ E +L + +D+L + KA+ EKA K L +L+K+++E +LQ+ DM+ GN+KS +EN E+MRQL++VD N+SML+K + QL N+LE K+M ++E+KERQS LGR+RNL+HE+DG+ AVYEEE+ AK++ RQ +KAE+E +LWR YE +GIAKIEELEN+K+KLQARLAECEGTVEN N KL+QL+K+K LQ+ I+ + + VD AN+ QM+KK++QFDK++ DWK KAD +++EL++SQ++CRNV+TELFRVKNGYE+ L+E+ + + + + + E+ RS E ++ +K+LE + EL++ALE A T QE +N ID+ +N I + R ++++ I ++ N R +Q LE+ + R R+ E ++SE A+ + ++ N RR LE E MD +L+ L+E + T+ + A + E+L Q Q L R+ L+ + ++L+ DD + NAM + K A+++ ++ IKEL ESELD EQRR D+ KNLKK ER+IKE++FQ EE+RKQ MQ+LV++LQ KLR FK+QIEEAEEIAA+NL K+RK QG+ EEAEERA L+EQA +K + RA
Sbjct: 1 MPGNEKIGSGTGVDPDPAPYLYVTFDMKRNDMSKPYDPKKSYWVPDGEGGFLEGLLESENGGKVVVMIQHEKRTLKKEQIQQVNPPKFEKCEDMSNLTYLNEASVLHNLKARYQAQLIYTYSGLFCVAINPYKRLPIYTNTTVKLYLGKRRTEVPPHLFAISDLAYRNMLDKGVCQSILVTGESGAGKTENTKKVISYFAMVGARDGPK-TSNKVSLEDQIVQTNPILESFGNAKTARNDNSSRFGKFIRIHFNSSGKLAGCDIETYLLEKARITFQQEVERSYHIFYQMMEP--AVPDLKKKCFLSNDIYDYHFVSMGKTKVPSIDDNEDLEFTHEAFRILCFGEENTYNVYRATAAVMHMGELKFKQKGREEQCEPDNPAQAKKVAALLGIEPESMMKAFCKPKIKVGTEWVTKGQNIDQSYHSVAGIARGLYERVFNFLVEKCNETLVDDTMKKVVFIGVLDIAGFEIFEFNGFEQLCINFCNEKLQQFFNHHMFVLEQEEYLKEGIDWEMVDFGMDLQSCITMFEKPLGILSILEEESLFPKATDKTFEDKLKANHLGKSPNFVKAITKHDKNAHFAIVHYAGTVSYNVTSWLEKNKDPLNDTVIELLKSGENKLINHVFRDHPGQGR-EEDGDQKGKKKKGGGAKTVSTFYTKQLNSLMTTLHATEPHFIRCIIPNTHKQPGQIEPALVLHQLTCNGVLEGIRICMRGFPNRMPYPEFIFRYGILQNGKP---NSSDEKVQAAAICTEMLNKEKYRIGMCKIFFRAGVLGYLEEVRDEKVTRMIRYMQGYCYGILRRRDFLKRKQQRDLLVVIQKLFRRYLTLRHWGWFSIIQKTKPLIGMLNIEEEINILEEAATKAVDEVQFEATEKKRLESANTRLMEEKMALLKRIEIEQGDIISLQERQAKAAAQKADLENQLDEMSEKIKAEENRKEELIKEKRAIEADVSNVRGDLSDLEVHVQKAEQERANKDHQLRSLNDDIASKDELISKLNKEKKHFLEINGKAVEELQNADEKVSHLTMVKNKLEQTLDDLEDNLERERRKRNEEEKQRRKIEGDVKLTQEAIIELEKGKKELDQCLARKERDIAELTGKLSEEQSGVSKLTKVIKETQARVEEMEEELEAERQARGKAERQRSELAREMEELTERIEEAGGATQAQMELNKKREAEVVRMRKDLEEINIQHEATIFSLKKKHQDAIGEMSEQIEQLNKLRAKLELDCGGIKLEYSDLTIFLDNLAKDKAAGEKAGKTLSTQLSDLNKKISEVNLQVNDMELGNRKSASENSELMRQLDDVDSNLSMLHKIRHQLNNELEQVKKMYDEESKERQSTLGRFRNLKHEFDGLAAVYEEEVTAKENFHRQCQKAEEETNLWRLKYEKDGIAKIEELENTKMKLQARLAECEGTVENLNTKLVQLEKSKNQLQENIEDITAKVDQANIINIQMDKKLKQFDKVVHDWKIKADNMSQELEYSQRDCRNVSTELFRVKNGYEECMGQLDEVKRENKMLSDDIRDIMEQISEGGRSIH------------------EIEKQRKRLETEKQELQAALEEAEATLEQEENKNARLQLEIDQVKNDI---EKRLQEKEEEFEITKK---NHHRMTEQIQETLEDESKAKAELMRCRKKLEADVSELEGALEQANHVHEENQRNIRRYQDLIQKANINLEEEQQAKDLARENHVNMDRRAHSLQNALEEARTILDQTDRARRSAEQEVGECHEQLSDLSMQNQSLAGARRKLEQEAENLRQDCDDMKGEARSCEERARNAMIDAAKLAEELRVEQEHTAHAEQARKMADALIKELQMKIDDVEVSSMKQGKKVCSKLESRIKELESELDAEQRRLGDNSKNLKKGERRIKEIEFQSEEERKQAGQMQELVDRLQNKLRAFKRQIEEAEEIAALNLAKYRKTQGDAEEAEERATLTEQAAAKLKSAARA 1923
BLAST of low quality protein: myosin heavy muscle vs. nr
Match: gi|1325277640|ref|XP_023323458.1| (myosin heavy chain, muscle-like [Eurytemora affinis]) HSP 1 Score: 2170.2 bits (5622), Expect = 0.000e+0 Identity = 1116/1933 (57.73%), Postives = 1433/1933 (74.13%), Query Frame = 0
Query: 54 MPGNVKLGSSNEPDPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGKKAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKSKKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSDKNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLK-CGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDFIDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHV----------------------------DNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRV------------------------KNGYE----DAANHLN-----------ELSQARQEIERRLAEKEEEFDAVRRSHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRATT 1918
MPG+VKLG + EPDPDP PYL VSME+KR+ KPYD KKSYW PD +GGF+E L++S++G KA+VLIGHEKKTFKSEQV QVNPPKFEKCEDM+NLT+LN+ASV WNLK RYQ KLIYTYSGLFCV VNPYKRFPIYT VKLYLGKRRNEVPPHL+AI++TAYRNML N NQSMLITGESGAGKTENTKKVISY A V A KK +KK SLEDQIV TNPILE++GNAKT+RNDNSSRFGKFIRIHFNASGKLAGCDIE+YLLEKSRIT QQEVERSYHIFYQ++Q PD+K C+L +DIYDY YVSQGK +V SIDD E+LE+T AF+I+ F++EE N YK AAVM MGEM+FKQKGR+EQCEPD ++ A KV LG+D E++MKA+CKP+IKVGTEWVTKGQN++QS AV GIAR ++DR+F++++EKCN+TL+D TMKKV F+ VLDIAGFEIF+YNGFEQI INF NEKLQQFFNHHMFV+EQ+EY+ EGIDW MVDFGMDLQACI MFEKPMG+ AILEEESLFPKATDKSFE+KLK + LGK F K +K+DKNAHFAI HYAG VSYN++GWLEKNKDP+ND+VVE+ K +N L+VH++ADHPGQ + KGKK KGSG KTVS+ Y QL SLMSTLH+TEPHFIRCIVPN +K PG +++ L++HQLTCNGVLEGIRICMRGFPNR+ YP+F RY IL K+ + T L + + E++RIGNTKVFFRAG L LEE RDDIV KL+RFLQG R+ F+K+ QR +I+VIQR FR ++ R+WGWF II+KTKP++G+ N EEE+++LE+ A A A+ + N +++LE+EN + ++ + K++ EQGDL YQE+ AK +TQKA EL+L + + KL E + S D K+ +E E ++++D +L +L K +EK RD +R LND++ DE +SKLNKEKKY+QE KASEDL+SAEDKV HLN VKSKLE+T+D ++ SLE+EKR K +++K RRK+EGDLK+ QEMVL++ER KRELE + R++ ++ + +L+ EQ G + QR IKE QARVEE+EEELEAERQ+R+KAERQ++DL RE DEL+ERL+E+ T AQ+ELNKKRE E+ ++RKD+EE IQQEST+LSL+KKHQDAI EMSEQ DQL K+KAK+E DK ++ + ++ R + D +V KA AEK K A L K++ E ++ L + D+ NK+ M+EN + +LE++ N+SML K KI L++QL+DAKRM ++E+KERQSLLGR+R LEHEYDG+ +E+E K++ R +K ++++WR YE + + KIEELE +KLKLQARLAE E T+EN N+KL+ L+KAK +Q++I+ MA V D +++ + +K+ R + K+ D +LD ++E R+++ E+ + KN E DA + L E++Q + +IE+R+ EKEEEF+ RR+H K +E MQ +E E K+KAEA RM+KKLE D+ ELESAL+HAN+ + E Q++I YQ +I+D ++F+ EQ K AR+ MLNAER+AH+ QNALEET+T+LEQADRAR+ AEQEL + NE ++DL+VQNQ L ++KR+LE ++ +LR + DE E TE+KAK+ MMDAA++++ELR EQ+ Q LE +++ ++A+ KDLQI+LDDAE NA+K GRK A K+++R +ELE+ELD EQRR D+ KNLKK ER IKE F+ EEDRK E M+DLV+KLQ ++R +KKQIEEAEEIA+ NL KFRKAQ + E+ ERAD+SEQA +KYR GR+ +
Sbjct: 1 MPGHVKLGKTGEPDPDPAPYLVVSMEMKREAQAKPYDSKKSYWCPDGEGGFLESLLESNEGGKAVVLIGHEKKTFKSEQVGQVNPPKFEKCEDMANLTFLNDASVFWNLKTRYQTKLIYTYSGLFCVVVNPYKRFPIYTPTVVKLYLGKRRNEVPPHLWAITETAYRNMLQNSKNQSMLITGESGAGKTENTKKVISYLAMV-ATTGKKSDTKKVSLEDQIVATNPILESYGNAKTSRNDNSSRFGKFIRIHFNASGKLAGCDIESYLLEKSRITQQQEVERSYHIFYQLLQPF--VPDLKAKCNLGDDIYDYSYVSQGKVTVASIDDNEELEYTDSAFDIIGFSQEEKWNCYKLTAAVMTMGEMRFKQKGRDEQCEPDAMDIAGKVASNLGIDAESVMKAFCKPRIKVGTEWVTKGQNIEQSMNAVGGIARAIFDRLFKWLIEKCNETLIDATMKKVNFVAVLDIAGFEIFEYNGFEQISINFVNEKLQQFFNHHMFVVEQQEYVSEGIDWVMVDFGMDLQACIIMFEKPMGLWAILEEESLFPKATDKSFEEKLKAS-LGKLPIFLKPQSKTDKNAHFAISHYAGLVSYNVTGWLEKNKDPVNDSVVEIFKSTATNALLVHLWADHPGQPLVAPKDDAKGKKKKGSGGKTVSSVYLVQLTSLMSTLHSTEPHFIRCIVPNTHKQPGAVEAPLIMHQLTCNGVLEGIRICMRGFPNRILYPDFKQRYTILAQGKVDAKTEEKKAASTILDMTEGFEAEKYRIGNTKVFFRAGALALLEEKRDDIVTKLIRFLQGQVYARFGRERFQKKSNQRNMIKVIQRQFRNYIKNRDWGWFVIIRKTKPMVGVPNPEEELRLLEEKAKTAYGAYEEQLNTKKQLEEENNLMVDEINQLKKKLNDEQGDLSSYQEKLAKLSTQKADLELQLEECESKLASEERNKASADDDKKSVEREFTNLKRDYADLTSKLEKVSAEKGKRDQVLRGLNDEVLYQDETISKLNKEKKYIQETVGKASEDLNSAEDKVTHLNEVKSKLEKTLDQMDGSLEKEKRMKYNIEKDRRKIEGDLKMCQEMVLDIERSKRELEQSIMRKDTELNTLMGKLDDEQNGVGRTQRIIKELQARVEEMEEELEAERQSRSKAERQRNDLSREYDELTERLDESCVTTAAQIELNKKREAEIMKMRKDVEECNIQQESTLLSLRKKHQDAIAEMSEQCDQLNKIKAKMEKDKVAVKMQLDDTRAANDHVVHEKAVAEKNLKALHAQLQSFMKKIDESAVVLGEYDSANKRQMSENSNLYSRLEDLLNNVSMLQKIKITLSSQLDDAKRMCDEESKERQSLLGRFRTLEHEYDGVKEHFEDEQQQKNEAGRHLQKLMADSNMWRCKYETDALGKIEELEMTKLKLQARLAENESTMENLNSKLVSLEKAKLQVQKDIEEMAHRVDQVNIMYNQAEKRVKLMDKVIAEWKTKADTISLELNNSQKECRNASAELFRVKNGYDEAASQLDDVRRENRSLSDEIKDLMEQISEGGRSIHEIEKQRKKLEAEKNELEQALGDAESALEQEENKYLRLSLEVNQVKADIEKRIQEKEEEFEGTRRNHAKVMEQMQCAIEQESKSKAEAIRMRKKLELDINELESALDHANMANMELQKSIKLYQERIKDKTMQFEAEQVAKDRARDYMLNAERKAHSSQNALEETKTMLEQADRARKQAEQELCDTNEQLADLTVQNQGLYSSKRKLESDLADLRSEGDEAAAEAHDTEEKAKRYMMDAAKLADELRSEQENAQILEKEKREMEARTKDLQIQLDDAETNAIKWGRKMAAKLDARCRELEAELDAEQRRSGDAYKNLKKAERGIKEYTFRSEEDRKNAERMRDLVDKLQQQIRTYKKQIEEAEEIASTNLAKFRKAQVDLTESCERADISEQACAKYRARGRSQS 1929
BLAST of low quality protein: myosin heavy muscle vs. nr
Match: gi|768433458|ref|XP_011558096.1| (PREDICTED: myosin heavy chain, muscle isoform X5 [Plutella xylostella]) HSP 1 Score: 2029.6 bits (5257), Expect = 0.000e+0 Identity = 1079/1937 (55.70%), Postives = 1386/1937 (71.55%), Query Frame = 0
Query: 67 DPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGK-KAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKS-KKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSD--KNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGA-KTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYE----------------------------DAANHLNELSQARQEIERRLAEKEE------------------------EFDAVRR------------------SHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRA------TTPNP 1921
DPDP PYL VS+E KR D KPYD KK+ WVPDD+ GF++G +++ G +VL G E K FK + V QVNPPK+EK EDMSNLTYLN+ASVL+NLK RY KLIYTYSGLFCVA+NPYKRFP+YT KLY GKRR+EVPPH+FAISD AY NML+N NQSMLITGESGAGKTENTKKVI+YFA VGA ++K + KK SLEDQ+VQTNP+LEAFGNAKT RNDNSSRFGKFIRIHF SGKLAG DIETYLLEK+R+ QQ +ERSYHIFYQMM S P +K++C LS D+YDYH ++QGKT++P++DD E+ T +AF+IL FT+EE ++YK A+VMHMG MKFKQ+GREEQ E D +E+ ++V +LGVD + + K KP+IKVG E+VT+G+N+ Q T +V + +G++DR+F+++V+KCN+TL D K+ FIGVLDIAGFEIF +NGFEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDL ACI + EKPMGIL+ILEEES+FPKATD +F +KL NHLGKS + K + AHFAI HYAG V YN+SGWLEKNKDPLNDTVV+ K G N L++ IFADHPGQS G K A +TVS+ Y+ QL++LM+TL +T+PHF+RCI+PN K PG IDS LV+HQLTCNGVLEGIRIC +GFPNRM YP+F RY IL+ + + DPKKI ++I +D E +RIG+TKVFFRAGVLG +EE+RDD + K++ ++Q G+L RK+F+K ++QR +QV+QRN RK+L LR W W+ + QK KPL+ + +E+E+ LE+ A KA AF EE R+ LE N +L E+ A++ +E +L QER+AK QK E +L D QD+L +E L K+ LE EV ++KD+ +LE + K+E +K T+DH++RNLND+IA+ DE+++KLNKEKK E K +E+L +AEDKVNHLN VK KLEQT+D+LEDSLEREK+ + DV+KQRRKVEGDLK+ QE V ++ER K+ELE +QR++++I + A+LE EQ +K Q+ IKE QAR+EELEEE+E+ERQARAKAE+Q+ DL REL+EL ERLEEA GAT+AQ+ELNKKRE EL +LR+DLEEA IQ EST+ +L+KKH DA+ EM EQ+DQL KLKAK E +++ E +LR +D + KA+ EK K Q L E+S + E + L D+D KK EN +++RQLEE + +S L+K K+ LT QLED KR+A++E++ER +LLG++RNLEH+ D + EEE K DL RQ KA EA +WR YE EG+A+ EELE +K KLQARLAE E T+E+ N K++ L+K K L E++ + VD A + EKK + FDKIIG+WK K D L ELD SQKECRN +TELFR+K YE + +++E+ +AR+ +E AEK+E E VR+ +HQ+ ++ MQ++LEAE K KAEA RMKKKLEAD+ ELE AL+HAN + E Q+NI +YQ QI+D Q ++EQ+ + ARE + +ERRA+ LQN LEE+RTLLEQADRARR AEQEL + +E +++LS Q SL A KR+LE E+ L DLDE+ E +E+KAKKAM+DAAR+++ELR EQ+ Q E RK L+ Q+K+LQ+RLD+AE NA+K G+KA QK+E R++ELE+ELDGEQRR AD+ KNL+K+ER+IKE+ FQ EEDRK HE MQDLV+KLQ K++ +K+QIEEAEEIAA+NL KFRKAQ E EEAEERADL+EQA+SK+R GRA +P P
Sbjct: 11 DPDPTPYLFVSLEQKRIDQSKPYDGKKACWVPDDKEGFLQGEIKATKGDLVTVVLPGGETKDFKKDLVGQVNPPKYEKAEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTFRCAKLYRGKRRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPNAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYLLEKARVISQQALERSYHIFYQMMS--GSVPGLKELCMLSNDVYDYHIIAQGKTTIPNVDDGEECTLTDQAFDILGFTQEEKNDVYKITASVMHMGGMKFKQRGREEQAEADGMEEGERVAKLLGVDCQDLYKNLLKPRIKVGNEFVTQGRNITQVTNSVGALCKGVFDRLFKWLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGINWTFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDATFVEKLNNNHLGKSAPYLKPKPPKPGCQAAHFAIGHYAGNVGYNISGWLEKNKDPLNDTVVDQFKKGQNKLLIEIFADHPGQSGDAAAAKGAGGKRAKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGLIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILNPAGVDK--ESDPKKIAQVILEATGLDPESYRIGHTKVFFRAGVLGQMEELRDDRLSKIMSWMQAYIRGYLSRKEFKKIQEQRLALQVVQRNLRKYLQLRTWPWWKLWQKVKPLLNVSRVEDELAKLEEKAAKAQEAFEKEEKLRKELEVLNAKLLEEKTALLSNLEGGGRELQDTQERAAKLQAQKNDLESQLRDTQDRLTQEEDARNQLFQNKKKLEQEVAGLKKDVEDLELAVQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETTQKTAEELQAAEDKVNHLNKVKQKLEQTLDELEDSLEREKKLRGDVEKQRRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSLVSKSQKQIKELQARIEELEEEVESERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMGEQLDQLNKLKAKAEKERSQYFSEVNDLRAGLDHVSNEKAAQEKMVKQLQHQLNEVSNKADEANRTLNDLDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNIREQVEEEAEGKADLQRQLSKANAEAQIWRSKYESEGVARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRATAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLE---AEKDELQAALEEAESALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQAQIKDLQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNELSAQAASLSAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRSEQEHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELTFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRGKGRAGSVARGVSPAP 1939
BLAST of low quality protein: myosin heavy muscle vs. nr
Match: gi|768433476|ref|XP_011558105.1| (PREDICTED: myosin heavy chain, muscle isoform X14 [Plutella xylostella]) HSP 1 Score: 2029.22 bits (5256), Expect = 0.000e+0 Identity = 1079/1936 (55.73%), Postives = 1384/1936 (71.49%), Query Frame = 0
Query: 67 DPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGK-KAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKS-KKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSD--KNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYE----------------------------DAANHLNELSQARQEIERRLAEKEE------------------------EFDAVR------------------RSHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRA------TTPNP 1921
DPDP PYL VS+E KR D KPYD KK+ WVPDD+ GF++G +++ G +VL G E K FK + V QVNPPK+EK EDMSNLTYLN+ASVL+NLK RY KLIYTYSGLFCVA+NPYKRFP+YT KLY GKRR+EVPPH+FAISD AY NML+N NQSMLITGESGAGKTENTKKVI+YFA VGA ++K + KK SLEDQ+VQTNP+LEAFGNAKT RNDNSSRFGKFIRIHF SGKLAG DIETYLLEK+R+ QQ +ERSYHIFYQMM S P +K++C LS D+YDYH ++QGKT++P++DD E+ T +AF+IL FT+EE ++YK A+VMHMG MKFKQ+GREEQ E D +E+ ++V +LGVD + + K KP+IKVG E+VT+G+N+ Q T +V + +G++DR+F+++V+KCN+TL D K+ FIGVLDIAGFEIF +NGFEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDL ACI + EKPMGIL+ILEEES+FPKATD +F +KL NHLGKS + K + AHFAI HYAG V YN+SGWLEKNKDPLNDTVV+ K G N L++ IFADHPGQS K G TVS+ YK QL++LM+TL +T+PHF+RCI+PN K PG IDS LV+HQLTCNGVLEGIRIC +GFPNRM YP+F RY IL+ + + DPKKI ++I +D E +RIG+TKVFFRAGVLG +EE+RDD + K++ ++Q G+L RK+F+K ++QR +QV+QRN RK+L LR W W+ + QK KPL+ + +E+E+ LE+ A KA AF EE R+ LE N +L E+ A++ +E +L QER+AK QK E +L D QD+L +E L K+ LE EV ++KD+ +LE + K+E +K T+DH++RNLND+IA+ DE+++KLNKEKK E K +E+L +AEDKVNHLN VK KLEQT+D+LEDSLEREK+ + DV+KQRRKVEGDLK+ QE V ++ER K+ELE +QR++++I + A+LE EQ +K Q+ IKE QAR+EELEEE+E+ERQARAKAE+Q+ DL REL+EL ERLEEA GAT+AQ+ELNKKRE EL +LR+DLEEA IQ EST+ +L+KKH DA+ EM EQ+DQL KLKAK E +++ E +LR +D + KA+ EK K Q L E+S + E + L D+D KK EN +++RQLEE + +S L+K K+ LT QLED KR+A++E++ER +LLG++RNLEH+ D + EEE K DL RQ KA EA +WR YE EG+A+ EELE +K KLQARLAE E T+E+ N K++ L+K K L E++ + VD A + EKK + FDKIIG+WK K D L ELD SQKECRN +TELFR+K YE + +++E+ +AR+ +E AEK+E E VR ++HQ+ ++ MQ++LEAE K KAEA RMKKKLEAD+ ELE AL+HAN + E Q+NI +YQ QI+D Q ++EQ+ + ARE + +ERRA+ LQN LEE+RTLLEQADRARR AEQEL + +E +++LS Q SL A KR+LE E+ L DLDE+ E +E+KAKKAM+DAAR+++ELR EQ+ Q E RK L+ Q+K+LQ+RLD+AE NA+K G+KA QK+E R++ELE+ELDGEQRR AD+ KNL+K+ER+IKE+ FQ EEDRK HE MQDLV+KLQ K++ +K+QIEEAEEIAA+NL KFRKAQ E EEAEERADL+EQA+SK+R GRA +P P
Sbjct: 11 DPDPTPYLFVSLEQKRIDQSKPYDGKKACWVPDDKEGFLQGEIKATKGDLVTVVLPGGETKDFKKDLVGQVNPPKYEKAEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTFRCAKLYRGKRRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPNAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYLLEKARVISQQALERSYHIFYQMMS--GSVPGLKELCMLSNDVYDYHIIAQGKTTIPNVDDGEECTLTDQAFDILGFTQEEKNDVYKITASVMHMGGMKFKQRGREEQAEADGMEEGERVAKLLGVDCQDLYKNLLKPRIKVGNEFVTQGRNITQVTNSVGALCKGVFDRLFKWLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGINWTFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDATFVEKLNNNHLGKSAPYLKPKPPKPGCQAAHFAIGHYAGNVGYNISGWLEKNKDPLNDTVVDQFKKGQNKLLIEIFADHPGQSGDAAAAKGGRGKKGGG-FATVSSAYKEQLNNLMTTLRSTQPHFVRCIIPNELKQPGLIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILNPAGVDK--ESDPKKIAQVILEATGLDPESYRIGHTKVFFRAGVLGQMEELRDDRLSKIMSWMQAYIRGYLSRKEFKKIQEQRLALQVVQRNLRKYLQLRTWPWWKLWQKVKPLLNVSRVEDELAKLEEKAAKAQEAFEKEEKLRKELEVLNAKLLEEKTALLSNLEGGGRELQDTQERAAKLQAQKNDLESQLRDTQDRLTQEEDARNQLFQNKKKLEQEVAGLKKDVEDLELAVQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETTQKTAEELQAAEDKVNHLNKVKQKLEQTLDELEDSLEREKKLRGDVEKQRRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSLVSKSQKQIKELQARIEELEEEVESERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMGEQLDQLNKLKAKAEKERSQYFSEVNDLRAGLDHVSNEKAAQEKMVKQLQHQLNEVSNKADEANRTLNDLDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNIREQVEEEAEGKADLQRQLSKANAEAQIWRSKYESEGVARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRATAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLE---AEKDELQAALEEAESALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQAQIKDLQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNELSAQAASLSAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRSEQEHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELTFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRGKGRAGSVARGVSPAP 1937
BLAST of low quality protein: myosin heavy muscle vs. nr
Match: gi|768433452|ref|XP_011558093.1| (PREDICTED: myosin heavy chain, muscle isoform X2 [Plutella xylostella]) HSP 1 Score: 2029.22 bits (5256), Expect = 0.000e+0 Identity = 1081/1937 (55.81%), Postives = 1384/1937 (71.45%), Query Frame = 0
Query: 67 DPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGK-KAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKS-KKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSD--KNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGA-KTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYE----------------------------DAANHLNELSQARQEIERRLAEKEE------------------------EFDAVRR------------------SHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRA------TTPNP 1921
DPDP PYL VS+E KR D KPYD KK+ WVPDD+ GF++G +++ G +VL G E K FK + V QVNPPK+EK EDMSNLTYLN+ASVL+NLK RY KLIYTYSGLFCVA+NPYKRFP+YT KLY GKRR+EVPPH+FAISD AY NML+N NQSMLITGESGAGKTENTKKVI+YFA VGA ++K + KK SLEDQ+VQTNP+LEAFGNAKT RNDNSSRFGKFIRIHF SGKLAG DIETYLLEK+R+ QQ +ERSYHIFYQMM S P +K++C LS DI+DY+ VSQGK ++P +DD E+ T +AF+IL FT+EE ++YK A+VMHMG MKFKQ+GREEQ E D +E+ ++V +LGVD + + K KP+IKVG E+VT+G+N+ Q T +V + +G++DR+F+++V+KCN+TL D K+ FIGVLDIAGFEIF +NGFEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDL ACI + EKPMGIL+ILEEES+FPKATD +F +KL NHLGKS + K + AHFAI HYAG V YN+SGWLEKNKDPLNDTVV+ K G N L++ IFADHPGQS G K A +TVS+ Y+ QL++LM+TL +T+PHF+RCI+PN K PG IDS LV+HQLTCNGVLEGIRIC +GFPNRM YP+F RY IL+ + + DPKKI ++I +D E +RIG+TKVFFRAGVLG +EE+RDD + K++ ++Q G+L RK+F+K ++QR +QV+QRN RK+L LR W W+ + QK KPL+ + +E+E+ LE+ A KA AF EE R+ LE N +L E+ A++ +E +L QER+AK QK E +L D QD+L +E L K+ LE EV ++KD+ +LE + K+E +K T+DH++RNLND+IA+ DE+++KLNKEKK E K +E+L +AEDKVNHLN VK KLEQT+D+LEDSLEREK+ + DV+KQRRKVEGDLK+ QE V ++ER K+ELE +QR++++I + A+LE EQ +K Q+ IKE QAR+EELEEE+E+ERQARAKAE+Q+ DL REL+EL ERLEEA GAT+AQ+ELNKKRE EL +LR+DLEEA IQ EST+ +L+KKH DA+ EM EQ+DQL KLKAK EHD+ E R ++D + R KA+ EK K Q L E+S + E + L D+D KK EN +++RQLEE + +S L+K K+ LT QLED KR+A++E++ER +LLG++RNLEH+ D + EEE K DL RQ KA EA +WR YE EG+A+ EELE +K KLQARLAE E T+E+ N K++ L+K K L E++ + VD A + EKK + FDKIIG+WK K D L ELD SQKECRN +TELFR+K YE + +++E+ +AR+ +E AEK+E E VR+ +HQ+ ++ MQ++LEAE K KAEA RMKKKLEAD+ ELE AL+HAN + E Q+NI +YQ QI+D Q ++EQ+ + ARE + +ERRA+ LQN LEE+RTLLEQADRARR AEQEL + +E +++LS Q SL A KR+LE E+ L DLDE+ E +E+KAKKAM+DAAR+++ELR EQ+ Q E RK L+ Q+K+LQ+RLD+AE NA+K G+KA QK+E R++ELE+ELDGEQRR AD+ KNL+K+ER+IKE+ FQ EEDRK HE MQDLV+KLQ K++ +K+QIEEAEEIAA+NL KFRKAQ E EEAEERADL+EQA+SK+R GRA +P P
Sbjct: 11 DPDPTPYLFVSLEQKRIDQSKPYDGKKACWVPDDKEGFLQGEIKATKGDLVTVVLPGGETKDFKKDLVGQVNPPKYEKAEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTFRCAKLYRGKRRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPNAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYLLEKARVISQQALERSYHIFYQMMS--GSVPGLKELCFLSNDIHDYYNVSQGKITIPGMDDGEECALTDQAFDILGFTQEEKNDVYKITASVMHMGGMKFKQRGREEQAEADGMEEGERVAKLLGVDCQDLYKNLLKPRIKVGNEFVTQGRNITQVTNSVGALCKGVFDRLFKWLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGINWTFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDATFVEKLNNNHLGKSAPYLKPKPPKPGCQAAHFAIGHYAGNVGYNISGWLEKNKDPLNDTVVDQFKKGQNKLLIEIFADHPGQSGDAAAAKGAGGKRAKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGLIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILNPAGVDK--ESDPKKIAQVILEATGLDPESYRIGHTKVFFRAGVLGQMEELRDDRLSKIMSWMQAYIRGYLSRKEFKKIQEQRLALQVVQRNLRKYLQLRTWPWWKLWQKVKPLLNVSRVEDELAKLEEKAAKAQEAFEKEEKLRKELEVLNAKLLEEKTALLSNLEGGGRELQDTQERAAKLQAQKNDLESQLRDTQDRLTQEEDARNQLFQNKKKLEQEVAGLKKDVEDLELAVQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETTQKTAEELQAAEDKVNHLNKVKQKLEQTLDELEDSLEREKKLRGDVEKQRRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSLVSKSQKQIKELQARIEELEEEVESERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMGEQLDQLNKLKAKAEHDRASCYSELNNTRAAVDQVAREKAAQEKMVKQLQHQLNEVSNKADEANRTLNDLDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNIREQVEEEAEGKADLQRQLSKANAEAQIWRSKYESEGVARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRATAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLE---AEKDELQAALEEAESALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQAQIKDLQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNELSAQAASLSAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRSEQEHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELTFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRGKGRAGSVARGVSPAP 1939
BLAST of low quality protein: myosin heavy muscle vs. nr
Match: gi|768433466|ref|XP_011558100.1| (PREDICTED: myosin heavy chain, muscle isoform X9 [Plutella xylostella]) HSP 1 Score: 2028.45 bits (5254), Expect = 0.000e+0 Identity = 1080/1937 (55.76%), Postives = 1383/1937 (71.40%), Query Frame = 0
Query: 67 DPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGK-KAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKS-KKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSD--KNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGA-KTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYE----------------------------DAANHLNELSQARQEIERRLAEKEE------------------------EFDAVRR------------------SHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRA------TTPNP 1921
DPDP PYL VS+E KR D KPYD KK+ WVPDD+ GF++G +++ G +VL G E K FK + V QVNPPK+EK EDMSNLTYLN+ASVL+NLK RY KLIYTYSGLFCVA+NPYKRFP+YT KLY GKRR+EVPPH+FAISD AY NML+N NQSMLITGESGAGKTENTKKVI+YFA VGA ++K + KK SLEDQ+VQTNP+LEAFGNAKT RNDNSSRFGKFIRIHF SGKLAG DIETYLLEK+R+ QQ +ERSYHIFYQMM S P +K +C LS DI DYH V+QGKT +P +DD E++ T +AF+IL FT+EE ++YK A+VMHMG MKFKQ+GREEQ E D +E+ ++V +LGVD + + K KP+IKVG E+VT+G+N+ Q T +V + +G++DR+F+++V+KCN+TL D K+ FIGVLDIAGFEIF +NGFEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDLQ CI + EKPMGIL+ILEEES+FPKATD +F +KL NHLGKS + K + AHFAI HYAG V YN+SGWLEKNKDPLNDTVV+ K G N L++ IFADHPGQS G K A +TVS+ Y+ QL++LM+TL +T+PHF+RCI+PN K PG IDS LV+HQLTCNGVLEGIRIC +GFPNRM YP+F RY IL+ + + DPKKI ++I +D E +RIG+TKVFFRAGVLG +EE+RDD + K++ ++Q G+L RK+F+K ++QR +QV+QRN RK+L LR W W+ + QK KPL+ + +E+E+ LE+ A KA AF EE R+ LE N +L E+ A++ +E +L QER+AK QK E +L D QD+L +E L K+ LE EV ++KD+ +LE + K+E +K T+DH++RNLND+IA+ DE+++KLNKEKK E K +E+L +AEDKVNHLN VK KLEQT+D+LEDSLEREK+ + DV+KQRRKVEGDLK+ QE V ++ER K+ELE +QR++++I + A+LE EQ +K Q+ IKE QAR+EELEEE+E+ERQARAKAE+Q+ DL REL+EL ERLEEA GAT+AQ+ELNKKRE EL +LR+DLEEA IQ EST+ +L+KKH DA+ EM EQ+DQL KLKAK E +++ E +LR +D + KA+ EK K Q L E+S + E + L D+D KK EN +++RQLEE + +S L+K K+ LT QLED KR+A++E++ER +LLG++RNLEH+ D + EEE K DL RQ KA EA +WR YE EG+A+ EELE +K KLQARLAE E T+E+ N K++ L+K K L E++ + VD A + EKK + FDKIIG+WK K D L ELD SQKECRN +TELFR+K YE + +++E+ +AR+ +E AEK+E E VR+ +HQ+ ++ MQ++LEAE K KAEA RMKKKLEAD+ ELE AL+HAN + E Q+NI +YQ QI+D Q ++EQ+ + ARE + +ERRA+ LQN LEE+RTLLEQADRARR AEQEL + +E +++LS Q SL A KR+LE E+ L DLDE+ E +E+KAKKAM+DAAR+++ELR EQ+ Q E RK L+ Q+K+LQ+RLD+AE NA+K G+KA QK+E R++ELE+ELDGEQRR AD+ KNL+K+ER+IKE+ FQ EEDRK HE MQDLV+KLQ K++ +K+QIEEAEEIAA+NL KFRKAQ E EEAEERADL+EQA+SK+R GRA +P P
Sbjct: 11 DPDPTPYLFVSLEQKRIDQSKPYDGKKACWVPDDKEGFLQGEIKATKGDLVTVVLPGGETKDFKKDLVGQVNPPKYEKAEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTFRCAKLYRGKRRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPNAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYLLEKARVISQQALERSYHIFYQMMS--GSVPGLKGICMLSNDIMDYHIVAQGKTVIPGVDDGEEMRLTDQAFDILGFTQEEKNDVYKITASVMHMGGMKFKQRGREEQAEADGMEEGERVAKLLGVDCQDLYKNLLKPRIKVGNEFVTQGRNITQVTNSVGALCKGVFDRLFKWLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQNCIDLIEKPMGILSILEEESMFPKATDATFVEKLNNNHLGKSAPYLKPKPPKPGCQAAHFAIGHYAGNVGYNISGWLEKNKDPLNDTVVDQFKKGQNKLLIEIFADHPGQSGDAAAAKGAGGKRAKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGLIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILNPAGVDK--ESDPKKIAQVILEATGLDPESYRIGHTKVFFRAGVLGQMEELRDDRLSKIMSWMQAYIRGYLSRKEFKKIQEQRLALQVVQRNLRKYLQLRTWPWWKLWQKVKPLLNVSRVEDELAKLEEKAAKAQEAFEKEEKLRKELEVLNAKLLEEKTALLSNLEGGGRELQDTQERAAKLQAQKNDLESQLRDTQDRLTQEEDARNQLFQNKKKLEQEVAGLKKDVEDLELAVQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETTQKTAEELQAAEDKVNHLNKVKQKLEQTLDELEDSLEREKKLRGDVEKQRRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSLVSKSQKQIKELQARIEELEEEVESERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMGEQLDQLNKLKAKAEKERSQYFSEVNDLRAGLDHVSNEKAAQEKMVKQLQHQLNEVSNKADEANRTLNDLDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNIREQVEEEAEGKADLQRQLSKANAEAQIWRSKYESEGVARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRATAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLE---AEKDELQAALEEAESALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQAQIKDLQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNELSAQAASLSAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRSEQEHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELTFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRGKGRAGSVARGVSPAP 1939
BLAST of low quality protein: myosin heavy muscle vs. nr
Match: gi|768433480|ref|XP_011558107.1| (PREDICTED: myosin heavy chain, muscle isoform X16 [Plutella xylostella]) HSP 1 Score: 2027.29 bits (5251), Expect = 0.000e+0 Identity = 1079/1936 (55.73%), Postives = 1384/1936 (71.49%), Query Frame = 0
Query: 67 DPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGK-KAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKS-KKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSD--KNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGAKTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYE----------------------------DAANHLNELSQARQEIERRLAEKEE------------------------EFDAVRR------------------SHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRA------TTPNP 1921
DPDP PYL VS+E KR D KPYD KK+ WVPDD+ GF++G +++ G +VL G E K FK + V QVNPPK+EK EDMSNLTYLN+ASVL+NLK RY KLIYTYSGLFCVA+NPYKRFP+YT KLY GKRR+EVPPH+FAISD AY NML+N NQSMLITGESGAGKTENTKKVI+YFA VGA ++K + KK SLEDQ+VQTNP+LEAFGNAKT RNDNSSRFGKFIRIHF SGKLAG DIETYLLEK+R+ QQ +ERSYHIFYQMM S P +K++C LS D+YDYH ++QGKT++P++DD E+ T +AF+IL FT+EE ++YK A+VMHMG MKFKQ+GREEQ E D +E+ ++V +LGVD + + K KP+IKVG E+VT+G+N+ Q T +V + +G++DR+F+++V+KCN+TL D K+ FIGVLDIAGFEIF +NGFEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDLQ CI + EKPMGIL+ILEEES+FPKATD +F +KL NHLGKS + K + AHFAI HYAG V YN+SGWLEKNKDPLNDTVV+ K G N L++ IFADHPGQS K G TVS+ YK QL++LM+TL +T+PHF+RCI+PN K PG IDS LV+HQLTCNGVLEGIRIC +GFPNRM YP+F RY IL+ + + DPKKI ++I +D E +RIG+TKVFFRAGVLG +EE+RDD + K++ ++Q G+L RK+F+K ++QR +QV+QRN RK+L LR W W+ + QK KPL+ + +E+E+ LE+ A KA AF EE R+ LE N +L E+ A++ +E +L QER+AK QK E +L D QD+L +E L K+ LE EV ++KD+ +LE + K+E +K T+DH++RNLND+IA+ DE+++KLNKEKK E K +E+L +AEDKVNHLN VK KLEQT+D+LEDSLEREK+ + DV+KQRRKVEGDLK+ QE V ++ER K+ELE +QR++++I + A+LE EQ +K Q+ IKE QAR+EELEEE+E+ERQARAKAE+Q+ DL REL+EL ERLEEA GAT+AQ+ELNKKRE EL +LR+DLEEA IQ EST+ +L+KKH DA+ EM EQ+DQL KLKAK E +++ E +LR +D + KA+ EK K Q L E+S + E + L D+D KK EN +++RQLEE + +S L+K K+ LT QLED KR+A++E++ER +LLG++RNLEH+ D + EEE K DL RQ KA EA +WR YE EG+A+ EELE +K KLQARLAE E T+E+ N K++ L+K K L E++ + VD A + EKK + FDKIIG+WK K D L ELD SQKECRN +TELFR+K YE + +++E+ +AR+ +E AEK+E E VR+ +HQ+ ++ MQ++LEAE K KAEA RMKKKLEAD+ ELE AL+HAN + E Q+NI +YQ QI+D Q ++EQ+ + ARE + +ERRA+ LQN LEE+RTLLEQADRARR AEQEL + +E +++LS Q SL A KR+LE E+ L DLDE+ E +E+KAKKAM+DAAR+++ELR EQ+ Q E RK L+ Q+K+LQ+RLD+AE NA+K G+KA QK+E R++ELE+ELDGEQRR AD+ KNL+K+ER+IKE+ FQ EEDRK HE MQDLV+KLQ K++ +K+QIEEAEEIAA+NL KFRKAQ E EEAEERADL+EQA+SK+R GRA +P P
Sbjct: 11 DPDPTPYLFVSLEQKRIDQSKPYDGKKACWVPDDKEGFLQGEIKATKGDLVTVVLPGGETKDFKKDLVGQVNPPKYEKAEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTFRCAKLYRGKRRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPNAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYLLEKARVISQQALERSYHIFYQMMS--GSVPGLKELCMLSNDVYDYHIIAQGKTTIPNVDDGEECTLTDQAFDILGFTQEEKNDVYKITASVMHMGGMKFKQRGREEQAEADGMEEGERVAKLLGVDCQDLYKNLLKPRIKVGNEFVTQGRNITQVTNSVGALCKGVFDRLFKWLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWTFIDFGMDLQNCIDLIEKPMGILSILEEESMFPKATDATFVEKLNNNHLGKSAPYLKPKPPKPGCQAAHFAIGHYAGNVGYNISGWLEKNKDPLNDTVVDQFKKGQNKLLIEIFADHPGQSGDAAAAKGGRGKKGGG-FATVSSAYKEQLNNLMTTLRSTQPHFVRCIIPNELKQPGLIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILNPAGVDK--ESDPKKIAQVILEATGLDPESYRIGHTKVFFRAGVLGQMEELRDDRLSKIMSWMQAYIRGYLSRKEFKKIQEQRLALQVVQRNLRKYLQLRTWPWWKLWQKVKPLLNVSRVEDELAKLEEKAAKAQEAFEKEEKLRKELEVLNAKLLEEKTALLSNLEGGGRELQDTQERAAKLQAQKNDLESQLRDTQDRLTQEEDARNQLFQNKKKLEQEVAGLKKDVEDLELAVQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETTQKTAEELQAAEDKVNHLNKVKQKLEQTLDELEDSLEREKKLRGDVEKQRRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSLVSKSQKQIKELQARIEELEEEVESERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMGEQLDQLNKLKAKAEKERSQYFSEVNDLRAGLDHVSNEKAAQEKMVKQLQHQLNEVSNKADEANRTLNDLDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNIREQVEEEAEGKADLQRQLSKANAEAQIWRSKYESEGVARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRATAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLE---AEKDELQAALEEAESALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQAQIKDLQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNELSAQAASLSAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRSEQEHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELTFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRGKGRAGSVARGVSPAP 1937
BLAST of low quality protein: myosin heavy muscle vs. nr
Match: gi|768433454|ref|XP_011558094.1| (PREDICTED: myosin heavy chain, muscle isoform X3 [Plutella xylostella]) HSP 1 Score: 2027.29 bits (5251), Expect = 0.000e+0 Identity = 1080/1937 (55.76%), Postives = 1383/1937 (71.40%), Query Frame = 0
Query: 67 DPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGK-KAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKS-KKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSD--KNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGA-KTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYE----------------------------DAANHLNELSQARQEIERRLAEKEE------------------------EFDAVRR------------------SHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRA------TTPNP 1921
DPDP PYL VS+E KR D KPYD KK+ WVPDD+ GF++G +++ G +VL G E K FK + V QVNPPK+EK EDMSNLTYLN+ASVL+NLK RY KLIYTYSGLFCVA+NPYKRFP+YT KLY GKRR+EVPPH+FAISD AY NML+N NQSMLITGESGAGKTENTKKVI+YFA VGA ++K + KK SLEDQ+VQTNP+LEAFGNAKT RNDNSSRFGKFIRIHF SGKLAG DIETYLLEK+R+ QQ +ERSYHIFYQMM S P +K +C LS DI DYH V+QGKT +P +DD E++ T +AF+IL FT+EE ++YK A+VMHMG MKFKQ+GREEQ E D +E+ ++V +LGVD + + K KP+IKVG E+VT+G+N+ Q T +V + +G++DR+F+++V+KCN+TL D K+ FIGVLDIAGFEIF +NGFEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDL ACI + EKPMGIL+ILEEES+FPKATD +F +KL NHLGKS + K + AHFAI HYAG V YN+SGWLEKNKDPLNDTVV+ K G N L++ IFADHPGQS G K A +TVS+ Y+ QL++LM+TL +T+PHF+RCI+PN K PG IDS LV+HQLTCNGVLEGIRIC +GFPNRM YP+F RY IL+ + + DPKKI ++I +D E +RIG+TKVFFRAGVLG +EE+RDD + K++ ++Q G+L RK+F+K ++QR +QV+QRN RK+L LR W W+ + QK KPL+ + +E+E+ LE+ A KA AF EE R+ LE N +L E+ A++ +E +L QER+AK QK E +L D QD+L +E L K+ LE EV ++KD+ +LE + K+E +K T+DH++RNLND+IA+ DE+++KLNKEKK E K +E+L +AEDKVNHLN VK KLEQT+D+LEDSLEREK+ + DV+KQRRKVEGDLK+ QE V ++ER K+ELE +QR++++I + A+LE EQ +K Q+ IKE QAR+EELEEE+E+ERQARAKAE+Q+ DL REL+EL ERLEEA GAT+AQ+ELNKKRE EL +LR+DLEEA IQ EST+ +L+KKH DA+ EM EQ+DQL KLKAK E +++ E +LR +D + KA+ EK K Q L E+S + E + L D+D KK EN +++RQLEE + +S L+K K+ LT QLED KR+A++E++ER +LLG++RNLEH+ D + EEE K DL RQ KA EA +WR YE EG+A+ EELE +K KLQARLAE E T+E+ N K++ L+K K L E++ + VD A + EKK + FDKIIG+WK K D L ELD SQKECRN +TELFR+K YE + +++E+ +AR+ +E AEK+E E VR+ +HQ+ ++ MQ++LEAE K KAEA RMKKKLEAD+ ELE AL+HAN + E Q+NI +YQ QI+D Q ++EQ+ + ARE + +ERRA+ LQN LEE+RTLLEQADRARR AEQEL + +E +++LS Q SL A KR+LE E+ L DLDE+ E +E+KAKKAM+DAAR+++ELR EQ+ Q E RK L+ Q+K+LQ+RLD+AE NA+K G+KA QK+E R++ELE+ELDGEQRR AD+ KNL+K+ER+IKE+ FQ EEDRK HE MQDLV+KLQ K++ +K+QIEEAEEIAA+NL KFRKAQ E EEAEERADL+EQA+SK+R GRA +P P
Sbjct: 11 DPDPTPYLFVSLEQKRIDQSKPYDGKKACWVPDDKEGFLQGEIKATKGDLVTVVLPGGETKDFKKDLVGQVNPPKYEKAEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTFRCAKLYRGKRRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPNAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYLLEKARVISQQALERSYHIFYQMMS--GSVPGLKGICMLSNDIMDYHIVAQGKTVIPGVDDGEEMRLTDQAFDILGFTQEEKNDVYKITASVMHMGGMKFKQRGREEQAEADGMEEGERVAKLLGVDCQDLYKNLLKPRIKVGNEFVTQGRNITQVTNSVGALCKGVFDRLFKWLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGINWTFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDATFVEKLNNNHLGKSAPYLKPKPPKPGCQAAHFAIGHYAGNVGYNISGWLEKNKDPLNDTVVDQFKKGQNKLLIEIFADHPGQSGDAAAAKGAGGKRAKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGLIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILNPAGVDK--ESDPKKIAQVILEATGLDPESYRIGHTKVFFRAGVLGQMEELRDDRLSKIMSWMQAYIRGYLSRKEFKKIQEQRLALQVVQRNLRKYLQLRTWPWWKLWQKVKPLLNVSRVEDELAKLEEKAAKAQEAFEKEEKLRKELEVLNAKLLEEKTALLSNLEGGGRELQDTQERAAKLQAQKNDLESQLRDTQDRLTQEEDARNQLFQNKKKLEQEVAGLKKDVEDLELAVQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETTQKTAEELQAAEDKVNHLNKVKQKLEQTLDELEDSLEREKKLRGDVEKQRRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSLVSKSQKQIKELQARIEELEEEVESERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMGEQLDQLNKLKAKAEKERSQYFSEVNDLRAGLDHVSNEKAAQEKMVKQLQHQLNEVSNKADEANRTLNDLDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNIREQVEEEAEGKADLQRQLSKANAEAQIWRSKYESEGVARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRATAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLE---AEKDELQAALEEAESALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQAQIKDLQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNELSAQAASLSAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRSEQEHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELTFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRGKGRAGSVARGVSPAP 1939
BLAST of low quality protein: myosin heavy muscle vs. nr
Match: gi|768433494|ref|XP_011558114.1| (PREDICTED: myosin heavy chain, muscle isoform X23 [Plutella xylostella]) HSP 1 Score: 2026.91 bits (5250), Expect = 0.000e+0 Identity = 1078/1932 (55.80%), Postives = 1383/1932 (71.58%), Query Frame = 0
Query: 67 DPDPMPYLAVSMEVKRQDMLKPYDPKKSYWVPDDQGGFVEGLVQSDDGK-KAIVLIGHEKKTFKSEQVAQVNPPKFEKCEDMSNLTYLNEASVLWNLKARYQAKLIYTYSGLFCVAVNPYKRFPIYTHATVKLYLGKRRNEVPPHLFAISDTAYRNMLSNGHNQSMLITGESGAGKTENTKKVISYFANVGAREEKKRKS-KKASLEDQIVQTNPILEAFGNAKTARNDNSSRFGKFIRIHFNASGKLAGCDIETYLLEKSRITFQQEVERSYHIFYQMMQKLDSGPDIKKVCHLSEDIYDYHYVSQGKTSVPSIDDKEDLEFTHEAFNILHFTEEETVNIYKSVAAVMHMGEMKFKQKGREEQCEPDDIEQAKKVGDILGVDPEAMMKAYCKPKIKVGTEWVTKGQNLDQSTQAVAGIARGLYDRVFRFIVEKCNQTLVDPTMKKVVFIGVLDIAGFEIFKYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYIHEGIDWAMVDFGMDLQACITMFEKPMGILAILEEESLFPKATDKSFEDKLKTNHLGKSGNFAKASTKSD--KNAHFAIVHYAGTVSYNLSGWLEKNKDPLNDTVVELLKCGSNNLVVHIFADHPGQSPLPEDKTKKGKKAKGSGA-KTVSTFYKTQLDSLMSTLHATEPHFIRCIVPNGNKMPGEIDSGLVLHQLTCNGVLEGIRICMRGFPNRMPYPEFCSRYAILDANKIAQLGSKDPKKITELICNDF-IDKERFRIGNTKVFFRAGVLGYLEEVRDDIVLKLVRFLQGACNGFLRRKDFEKRRKQRELIQVIQRNFRKFLSLRNWGWFSIIQKTKPLIGMINIEEEIKILEDAANKATNAFGSEENERQRLEKENKQLQEDTLAMMKRIETEQGDLVQYQERSAKAATQKACYELELSDNQDKLEREMQRNTSLQDQKRGLESEVGHIRKDMVELEDQLTKAESEKTTRDHKMRNLNDDIANLDEILSKLNKEKKYVQENNSKASEDLHSAEDKVNHLNMVKSKLEQTMDDLEDSLEREKRGKNDVDKQRRKVEGDLKVQQEMVLEMERGKRELESVVQRRERDIVEMNARLEAEQGGYAKQQRNIKETQARVEELEEELEAERQARAKAERQKHDLGRELDELSERLEEASGATTAQMELNKKREMELHRLRKDLEEATIQQESTILSLKKKHQDAIGEMSEQMDQLGKLKAKVEHDKTLIQRETEELRVSMDDLVRSKASAEKANKGFQAHLGELSKRMAEGSLQLADMDNGNKKSMAENGEIMRQLEEVDGNISMLNKTKIQLTNQLEDAKRMAEDEAKERQSLLGRYRNLEHEYDGMNAVYEEELAAKDDLARQAKKAEDEAHLWRQTYEVEGIAKIEELENSKLKLQARLAECEGTVENQNNKLIQLDKAKTALQQEIDSMASHVDNANMQYSQMEKKIRQFDKIIGDWKHKADGLTEELDHSQKECRNVATELFRVKNGYE----------------------------DAANHLNELSQARQEIERRLAEKEE------------------------EFDAVR------------------RSHQKGIEMMQSNLEAEMKAKAEAQRMKKKLEADVMELESALEHANVTHQENQRNIDKYQNQIRDSQLRFDDEQKVKAIARENMLNAERRAHTLQNALEETRTLLEQADRARRAAEQELSECNEAMSDLSVQNQSLGANKRRLEGEMDNLRQDLDEMKMETMMTEDKAKKAMMDAARISEELRMEQDQTQHLENDRKYLDAQVKDLQIRLDDAEMNAMKNGRKAAQKMESRIKELESELDGEQRRFADSMKNLKKNERKIKEMDFQEEEDRKQHEHMQDLVEKLQIKLRNFKKQIEEAEEIAAMNLTKFRKAQGETEEAEERADLSEQALSKYRVMGRATTPNPG 1922
DPDP PYL VS+E KR D KPYD KK+ WVPDD+ GF++G +++ G +VL G E K FK + V QVNPPK+EK EDMSNLTYLN+ASVL+NLK RY KLIYTYSGLFCVA+NPYKRFP+YT KLY GKRR+EVPPH+FAISD AY NML+N NQSMLITGESGAGKTENTKKVI+YFA VGA ++K + KK SLEDQ+VQTNP+LEAFGNAKT RNDNSSRFGKFIRIHF SGKLAG DIETYLLEK+R+ QQ +ERSYHIFYQMM S P +K++C LS DI+DY+ VSQGK ++P +DD E+ T +AF+IL FT+EE ++YK A+VMHMG MKFKQ+GREEQ E D +E+ ++V +LGVD + + K KP+IKVG E+VT+G+N+ Q T +V + +G++DR+F+++V+KCN+TL D K+ FIGVLDIAGFEIF +NGFEQ+CINF NEKLQQFFNHHMFVLEQEEY EGI+W +DFGMDL ACI + EKPMGIL+ILEEES+FPKATD +F +KL NHLGKS + K + AHFAI HYAG V YN+SGWLEKNKDPLNDTVV+ K G N L++ IFADHPGQS G K A +TVS+ Y+ QL++LM+TL +T+PHF+RCI+PN K PG IDS LV+HQLTCNGVLEGIRIC +GFPNRM YP+F RY IL+ + + DPKKI ++I +D E +RIG+TKVFFRAGVLG +EE+RDD + K++ ++Q G+L RK+F+K ++QR +QV+QRN RK+L LR W W+ + QK KPL+ + +E+E+ LE+ A KA AF EE R+ LE N +L E+ A++ +E +L QER+AK QK E +L D QD+L +E L K+ LE EV ++KD+ +LE + K+E +K T+DH++RNLND+IA+ DE+++KLNKEKK E K +E+L +AEDKVNHLN VK KLEQT+D+LEDSLEREK+ + DV+KQRRKVEGDLK+ QE V ++ER K+ELE +QR++++I + A+LE EQ +K Q+ IKE QAR+EELEEE+E+ERQARAKAE+Q+ DL REL+EL ERLEEA GAT+AQ+ELNKKRE EL +LR+DLEEA IQ EST+ +L+KKH DA+ EM EQ+DQL KLKAK E +++ E +LR +D + KA+ EK K Q L E+S + E + L D+D KK EN +++RQLEE + +S L+K K+ LT QLED KR+A++E++ER +LLG++RNLEH+ D + EEE K DL RQ KA EA +WR YE EG+A+ EELE +K KLQARLAE E T+E+ N K++ L+K K L E++ + VD A + EKK + FDKIIG+WK K D L ELD SQKECRN +TELFR+K YE + +++E+ +AR+ +E AEK+E E VR ++HQ+ ++ MQ++LEAE K KAEA RMKKKLEAD+ ELE AL+HAN + E Q+NI +YQ QI+D Q ++EQ+ + ARE + +ERRA+ LQN LEE+RTLLEQADRARR AEQEL + +E +++LS Q SL A KR+LE E+ L DLDE+ E +E+KAKKAM+DAAR+++ELR EQ+ Q E RK L+ Q+K+LQ+RLD+AE NA+K G+KA QK+E R++ELE+ELDGEQRR AD+ KNL+K+ER+IKE+ FQ EEDRK HE MQDLV+KLQ K++ +K+QIEEAEEIAA+NL KFRKAQ E EEAEERADL+EQA+SK+R GRA + G
Sbjct: 11 DPDPTPYLFVSLEQKRIDQSKPYDGKKACWVPDDKEGFLQGEIKATKGDLVTVVLPGGETKDFKKDLVGQVNPPKYEKAEDMSNLTYLNDASVLYNLKQRYYHKLIYTYSGLFCVAINPYKRFPVYTFRCAKLYRGKRRSEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASQKKDPNAEKKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPSGKLAGADIETYLLEKARVISQQALERSYHIFYQMMS--GSVPGLKELCFLSNDIHDYYNVSQGKITIPGMDDGEECALTDQAFDILGFTQEEKNDVYKITASVMHMGGMKFKQRGREEQAEADGMEEGERVAKLLGVDCQDLYKNLLKPRIKVGNEFVTQGRNITQVTNSVGALCKGVFDRLFKWLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGINWTFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDATFVEKLNNNHLGKSAPYLKPKPPKPGCQAAHFAIGHYAGNVGYNISGWLEKNKDPLNDTVVDQFKKGQNKLLIEIFADHPGQSGDAAAAKGAGGKRAKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGLIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILNPAGVDK--ESDPKKIAQVILEATGLDPESYRIGHTKVFFRAGVLGQMEELRDDRLSKIMSWMQAYIRGYLSRKEFKKIQEQRLALQVVQRNLRKYLQLRTWPWWKLWQKVKPLLNVSRVEDELAKLEEKAAKAQEAFEKEEKLRKELEVLNAKLLEEKTALLSNLEGGGRELQDTQERAAKLQAQKNDLESQLRDTQDRLTQEEDARNQLFQNKKKLEQEVAGLKKDVEDLELAVQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETTQKTAEELQAAEDKVNHLNKVKQKLEQTLDELEDSLEREKKLRGDVEKQRRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSLVSKSQKQIKELQARIEELEEEVESERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMGEQLDQLNKLKAKAEKERSQYFSEVNDLRAGLDHVSNEKAAQEKMVKQLQHQLNEVSNKADEANRTLNDLDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNIREQVEEEAEGKADLQRQLSKANAEAQIWRSKYESEGVARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRATAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLE---AEKDELQAALEEAESALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQAQIKDLQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNELSAQAASLSAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRSEQEHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELTFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQAISKFRGKGRAGSVARG 1934 The following BLAST results are available for this feature:
BLAST of low quality protein: myosin heavy muscle vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 25
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BLAST of low quality protein: myosin heavy muscle vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of low quality protein: myosin heavy muscle vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold1366_size45417:2947..17047- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold1366_size45417-snap-gene-0.5 ID=maker-scaffold1366_size45417-snap-gene-0.5|Name=low quality protein: myosin heavy muscle|organism=Tigriopus kingsejongensis|type=gene|length=14101bp|location=Sequence derived from alignment at scaffold1366_size45417:2947..17047- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'low quality protein: myosin heavy muscle' has the following synonyms
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