BLAST of cytochrome p450 cyp12a2-like vs. L. salmonis genes
Match: EMLSAG00000005044 (supercontig:LSalAtl2s:LSalAtl2s263:255058:256494:-1 gene:EMLSAG00000005044 transcript:EMLSAT00000005044 description:"augustus_masked-LSalAtl2s263-processed-gene-2.2")

HSP 1 Score: 208.379 bits (529), Expect = 3.540e-61
Identity = 133/431 (30.86%), Postives = 224/431 (51.97%), Query Frame = 0

Query:  105 QYDTEGVGLLRMSAPALNRAGQGR------VVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPSCYAYGFEGSCAIALDQRMGLFQAHPDP--EMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILSHPDHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGS--EERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLE 525
             YDTE +  L  S        + +      VV+L  P    IAY+  G +P+R   F  ++++R S+ +++   +G+L  +G  W++ RS VQ  ++RP+    Y   V  +  DF++ +     +  DG    DLL   Y +G E +  +A D+R+G+     D   E  E +  V+ I   L + +  +  W+    L   YRQ+   L+ + +     +D+  + I+ K   S      +TSV+E L    G + +V  V++ D ++AG+DT    + F F+++A N + Q  L  EI S  D      +    YL+A LKE  RL P    +VR+ Q    L  + I   ++ ++  ++M  DP++V+D  EF+PER+++     ER+ PF SLPFG+GPR+CVGKRFA+LEM +L+ +++  ++LE
Sbjct:   33 NYDTEIIPFLERSRLVYGDIYKFKAPLGPDVVVLFKPHHAEIAYRSAGKWPNR-PGFHAIQQFR-SQKELYSNNSGLLSSQGQDWYDFRSKVQTALLRPRLIKAYHQRVNGVVEDFIKDIFEN--TLEDGKASADLLEYLYQWGLESTGVLAFDRRLGVLNPSLDSSSESQEIINLVQEIFS-LSQYLERNMVWKYIGLLHGKYRQITKCLNRLEELTIKYVDEKIEMIERKGSCS------DTSVIENL-YHAGCDKNVLCVLALDMLMAGVDTAANEISFAFHYIAQNQKVQEKLFNEIRS-LDIDDPNFVENAVYLRATLKEVLRLKPTAYVNVRMCQNSIELDDYLIQEGSMILFCHIVMCHDPRFVNDPLEFRPERYIKSHPHYERLQPFTSLPFGYGPRVCVGKRFANLEMGLLLARIISKYKLE 450          

BLAST of cytochrome p450 cyp12a2-like vs. L. salmonis genes
Match: EMLSAG00000009224 (supercontig:LSalAtl2s:LSalAtl2s588:244024:246336:1 gene:EMLSAG00000009224 transcript:EMLSAT00000009224 description:"augustus_masked-LSalAtl2s588-processed-gene-2.0")

HSP 1 Score: 158.303 bits (399), Expect = 3.014e-42
Identity = 126/505 (24.95%), Postives = 232/505 (45.94%), Query Frame = 0

Query:   56 LASTTSTLRPFEAIPAVPGAWPVVGNFFRLGEKPHGWPRLWSNITRLQSQYDTEGVGLLRMSAPAL--NRAGQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPSCYAYGFEGSCAIALDQRMGLFQAHPDPEMLEQLARVRVIIEKLPELVVGSPWWRLWP-SLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEI---LSHPDHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDF-SLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLR--------GSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWVGSADPMETLWKFTNVPSL 545
             + T S   P   IP  P A+P+ G+ ++  +    W   W  + +++  +++      R   P +  N      ++ L + +++      +  YP R  + E    +R SRP+++    G++   GP WH +RS + + + +  +   Y+  +  IA D    + R++    +   ++D     Y  G E  C +AL++RMG        +    L  ++       E + G PWW   P + S  +  +  + D +     + ID+    ++ K      D   E S++ +L+  N     V   +  D+I AG+DT G ++ F    LA  PEKQ  L+ EI   L   ++IT++++ +M+YLK C+ ES RL P      RI  E+  +  G  +P  ++ +    + S   +  + + EF PERWL          +    N  + +PFG G R+C GK+ A+ E+ ++  K+++NF L    ++D  +  ++F  +PSL
Sbjct:   56 FSKTPSIKNPLMNIPG-PRAFPIFGSSWQYSD----W---WFGMYKMEKYHESNEDKYARY-GPVVRENVLWNFPLIHLFEKEEIEKVLNSKSKYPLRPPN-EADVYYRNSRPELYN-NIGMVNLNGPSWHTLRSTLTKPLTKSSTITNYVAHMNLIADDLTSLIKRRQN---EQGIIKDFQDIVYRAGLETVCTVALERRMGFLADTIALDTARILNAIQGYQSSSNESMYGXPWWXYVPETFSGXFTSLVKHKDYLFGTFGAMIDE--NLVQNK------DFCDEESIIHQLVSGNVDIKDVKASV-VDYITAGVDTIGNTVIFALLLLAKYPEKQKKLQAEIERVLGSENYITKENIQEMKYLKGCVNESFRLYPTASQIARILDEEIITSNGHSLPPGSVVLCHQRIASLQEQNFTRAKEFIPERWLEXEAVTEFGTTNYAHNKSLVMPFGFGKRICPGKKLAEQEIYIMTAKLMKNFNLRLHDASD-FDVEFRFLLMPSL 536          

BLAST of cytochrome p450 cyp12a2-like vs. L. salmonis genes
Match: EMLSAG00000005374 (supercontig:LSalAtl2s:LSalAtl2s28:147448:149791:1 gene:EMLSAG00000005374 transcript:EMLSAT00000005374 description:"maker-LSalAtl2s28-augustus-gene-1.45")

HSP 1 Score: 108.997 bits (271), Expect = 3.746e-26
Identity = 67/244 (27.46%), Postives = 127/244 (52.05%), Query Frame = 0

Query:  286 GSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGET--SVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILSHP-DHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQ-EDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLE 525
            G P W+++ +    Y+ +  + + I +        + + I+ K +N   + +     ++L++  + +  +      M  D +LAG+DT+  + GF+ Y+LA  P+ Q  LR+ I   P DH    +L    + +A LKE+ RL P++    RI Q  D    G+ +P  T+ +  + +  R  KY      F P+RW+    +  +P++ LPFG GPRMC+G+R A++ +  L+I++L+ + ++
Sbjct:  117 GPPLWKVFET--KEYKALRESQNYIENI-------SLKFIRNKMNNLNTNPLSNNHLTLLDQYFVMDKLDEKDILTMVCDSLLAGIDTSSYTTGFMLYYLAKYPQVQARLRETIHELPLDH----NLKNFSFGRAVLKETLRLNPVSVGIGRICQTSDAVFSGYSVPKGTVLVSQNQISCRLEKYFDRPHVFNPKRWM-DKNQNYHPYLVLPFGFGPRMCIGRRIAEMSILHLLIELLKKYEIK 346          

BLAST of cytochrome p450 cyp12a2-like vs. L. salmonis genes
Match: EMLSAG00000009405 (supercontig:LSalAtl2s:LSalAtl2s5:994028:996076:1 gene:EMLSAG00000009405 transcript:EMLSAT00000009405 description:"augustus_masked-LSalAtl2s5-processed-gene-10.0")

HSP 1 Score: 100.908 bits (250), Expect = 6.903e-23
Identity = 88/338 (26.04%), Postives = 150/338 (44.38%), Query Frame = 0

Query:  204 LDPVQTIASDFVEFLHRQRRSSPDGATVQ-------DLLPSCYAYGFEGSCAIALDQRMGLFQAHPDPEMLEQLARV---RVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTK-ADNSKNDEIGETSVLEKLILKNG------WNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILSHPDHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRL 524
            ++P+  I +DF+  +  Q  S       Q       D++ SC AYG + + +              D E+L+   ++    V+ + LP  +       L+P +          +DI G+  +  I  A   I  +  +N K  +    +VL ++   N        N+ +    +  F +AG  TT  SL  L Y LA NPE Q  L QEILS  D    +  ++  Y  A +KE+ RL P    H R+   D  ++G  I   T+           P++  + +EF+PER+ + +   + P    PFG G R+C+  RFA +E+Q+++ K+++ F +
Sbjct:  140 MEPLLEITNDFLFHIESQTTSGKSIKIKQKFHGLTLDVINSC-AYGIKTNSS-----------QDSDHELLKHAKKLLEDAVVTKNLPTSMF-ILLLNLFPGM-------IKYIDIFGNAYEELIKIANSIIANRETENIKKKDF--INVLVEMRKNNNECPNELLNNEIITAQAVIFFVAGYVTTSHSLCCLIYVLATNPEIQDQLYQEILSSED----QGFDEHSYTSAVIKEALRLYPPATMHTRVCTHDTEVEGIFIKNGTIIEMPIYASHYYPEFFPNPEEFKPERFFKENNSEIIPDTFRPFGDGNRICIAYRFAMMELQIVIEKLVKKFEI 451          

BLAST of cytochrome p450 cyp12a2-like vs. L. salmonis genes
Match: EMLSAG00000003403 (supercontig:LSalAtl2s:LSalAtl2s187:296091:300113:-1 gene:EMLSAG00000003403 transcript:EMLSAT00000003403 description:"maker-LSalAtl2s187-augustus-gene-3.13")

HSP 1 Score: 95.5153 bits (236), Expect = 8.750e-21
Identity = 56/222 (25.23%), Postives = 115/222 (51.80%), Query Frame = 0

Query:  333 KNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILSHPDHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWVGSADPMETLWKFTNVPSLKLDFKLHEL 554
            KN  +   S L  +++  G +      ++ DF++A +DTT  S  ++ + ++   +  YI  +  +S  + +          L+ C++E++RL P++PF  R+  +D  ++G ++    L ++S   MSR+P Y +  + F P+RW    +   + F S+PFG   R C+GK+ A+L M+ L+  ++Q +++  + + +  E + KF  +P   +   L  +
Sbjct:  245 KNTNVNSKS-LSGVLISQGVSYEDVERIAMDFVIAAVDTTSISTLWILHLIS---QDTYIQNEIRISFDNQL----------LRNCMREASRLYPVSPFITRVLDKDILIEGSKLRTGQLILFSIYTMSRNPNYFTKPEHFLPQRW----DITPSSFASIPFGFRARSCIGKKLAELSMETLIKGLIQRYKIHCL-NYEKAEMIMKFIGLPDRTIKIGLEHI 447          

BLAST of cytochrome p450 cyp12a2-like vs. L. salmonis genes
Match: EMLSAG00000005269 (supercontig:LSalAtl2s:LSalAtl2s281:237457:238966:1 gene:EMLSAG00000005269 transcript:EMLSAT00000005269 description:"maker-LSalAtl2s281-snap-gene-2.7")

HSP 1 Score: 93.9745 bits (232), Expect = 1.008e-20
Identity = 86/331 (25.98%), Postives = 148/331 (44.71%), Query Frame = 0

Query:  204 LDPVQTIASDFVEFLHRQRRSSPDGATVQ----------DLLPSCYAYGFEGSCAIALDQRMGLFQAHPDPEMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILSHPDHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRL 524
            ++PV  IA+ FV ++  Q   S +G +++          D++ +C AYG + + +             PD E+L   +++ +    + + V+ S    L+ S    ++ V    DI G      I  A   IKT+          ET  +EK   K+  N  V              TT  +L  L Y LA +PE Q  L +EI+S  D    K  ++  Y  A +KE+ R+ P    H+R   +D  ++G  I   T+          +P++    +EF+PER+ + +   + P    PFG G R C+  RFA +E+Q+++ K+++ F +
Sbjct:  135 MEPVSEIANQFVAYIETQ---SSEGKSIKIKHKFQGLTLDIINAC-AYGIKTNSS-----------EEPDHELLRH-SKMLLNDSFIAKNVITSVLILLFNSFPGMFKYV----DIFGKANDELIKIANSIIKTR----------ETHNIEK---KDFLNVLVQMRKK------SYSTTSMTLCCLTYILATHPEIQEKLYKEIVSSQD----KDFSEHSYTSAVIKETLRMYPPVTLHLRACVKDTEVEGILIKNGTIVEMPIYASHYNPEFFPTPNEFKPERFFQENNLEIIPNTYRPFGDGNRSCIAYRFAMMELQLILEKLVKKFEI 422          

BLAST of cytochrome p450 cyp12a2-like vs. L. salmonis genes
Match: EMLSAG00000012088 (supercontig:LSalAtl2s:LSalAtl2s865:171159:172708:-1 gene:EMLSAG00000012088 transcript:EMLSAT00000012088 description:"maker-LSalAtl2s865-snap-gene-0.12")

HSP 1 Score: 93.5893 bits (231), Expect = 1.646e-20
Identity = 86/339 (25.37%), Postives = 160/339 (47.20%), Query Frame = 0

Query:  204 LDPVQTIASDFVEFLHRQRRSSPDGATVQ----------DLLPSCYAYGFEGSCAIALDQRMGLFQAHPDPEMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNS--KNDEIGETSVLEKLILKNG------WNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILSHPDHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRL 524
            ++PV  IA+ FV ++  Q   S +G +++          D++ +C AYG + + +             PD E+L   +++ +    + + V+ S    L+ S    ++ V    D+ G      I  A   I+T+  ++  K D +   +VL ++  K+        N  +    +  F +AG  TT  +L  L Y LA +PE Q  L +EI+S  D    K  ++  Y  A +KE+ R+ P    H+R+  +D  ++G  I   T+          +P++  + +EF+PER+ + +   + P    PFG+G R C+  RFA +E Q+++ K+++ F +
Sbjct:  152 MEPVSDIANQFVAYIETQ---SSEGKSIKIQDKFQGLTLDIINAC-AYGIKTNSS-----------EEPDHELLRH-SKMLLNDSFITKNVITSVLILLFNSFPGMFKYV----DMFGKANDELIKIANSIIETRESHNIEKKDFL---NVLVQMRKKSHEDPNTLLNDEIITAQAIIFFIAGYSTTSTTLCCLTYMLATHPEIQEKLYKEIVSSED----KDFSEHSYTSAVIKETLRMYPPVALHLRVCAKDTEVEGIPIKKGTIIEMPIYASHYNPEFFPNPNEFKPERFFQENNLEIIPNTYRPFGNGNRSCIAYRFAMMEXQLILEKLVKKFEI 463          

BLAST of cytochrome p450 cyp12a2-like vs. L. salmonis genes
Match: EMLSAG00000008058 (supercontig:LSalAtl2s:LSalAtl2s47:1295633:1309112:-1 gene:EMLSAG00000008058 transcript:EMLSAT00000008058 description:"maker-LSalAtl2s47-augustus-gene-13.9")

HSP 1 Score: 88.1965 bits (217), Expect = 1.033e-18
Identity = 62/221 (28.05%), Postives = 105/221 (47.51%), Query Frame = 0

Query:  312 DFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSF--DFILAGLDTTGASLGFLFYHLAANPEKQYILRQEI---LSHPDHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGS-EERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEW 526
            +F++        A+K     ++N+ + E   L    L    + S+  V++     +  GL TT   L F  Y LA NP  Q  L +EI   + +   IT   L ++ +      E +R+T            D  L G+++P  T    +   +++  ++  D    +PERWLRG     ++P+  LPF HG RMC+G+R A+ +M V+M+ +L+ FR E+
Sbjct:  348 EFIRLAYSFVETALKDLKYMTENNSVREKEFLFLRFLLGKSDLSLANVITLIISLLTDGLSTTVPVLLFNLYSLAKNPSSQEKLYEEIQRVVKNDSDITTAHLKEILFPSGT--EVSRVT----------DHDLILSGYEVPKGTRVDLNHGPINKSEEFYEDPLIHKPERWLRGKFPSNIHPYQYLPFSHGTRMCIGRRLAEQDMNVVMVTLLRQFRFEY 556          

HSP 2 Score: 70.8626 bits (172), Expect = 3.243e-13
Identity = 43/126 (34.13%), Postives = 65/126 (51.59%), Query Frame = 0

Query:  402 DHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGS-EERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEW 526
            + + EK    +R+L    K   RL P      R+   D  L G+++P  TL   +    ++  +   D    +PERWLRG      +P+  LPF  G RMCVG+R A+ +M V+MI +L+ FR E+
Sbjct:  191 NSLREKDFLFLRFLLG--KSDLRLFPNGTEVSRVTDHDLILSGYEVPKGTLVDLNPGPANKSEEIYEDPLIHKPERWLRGKIPLESHPYQLLPFSKGTRMCVGRRLAEQDMNVVMITLLRQFRFEY 314          

BLAST of cytochrome p450 cyp12a2-like vs. L. salmonis genes
Match: EMLSAG00000007328 (supercontig:LSalAtl2s:LSalAtl2s412:195302:204629:-1 gene:EMLSAG00000007328 transcript:EMLSAT00000007328 description:"maker-LSalAtl2s412-snap-gene-2.12")

HSP 1 Score: 66.6254 bits (161), Expect = 6.873e-12
Identity = 42/145 (28.97%), Postives = 70/145 (48.28%), Query Frame = 0

Query:  405 TEKSLNQMRYLKACLKESARLTPITPFHV-RIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEERMNPFVS-LPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEW-VGSA--DPMETLWKFTNVPS 544
            T+  ++++ Y  A L E  RL  + P  + R   +D  + G+ IP  T    + +   RDP +  +  EF+PER+L     R+      LPFG G R+C+G+  A  ++ +  I +LQN  +   +G    DP       T +P+
Sbjct:  291 TQDCISELPYTHATLMEVQRLASVGPMSLPRYTTKDIDVNGYSIPEGTEIYSNFMAFHRDPDFFPEPLEFKPERFLTEDGSRVKKDEHFLPFGLGRRICMGESLARHQLIIFFIMILQNLNISVPIGQEKPDPESCFMGITRIPT 435          

BLAST of cytochrome p450 cyp12a2-like vs. L. salmonis genes
Match: EMLSAG00000002359 (supercontig:LSalAtl2s:LSalAtl2s144:369128:376155:1 gene:EMLSAG00000002359 transcript:EMLSAT00000002359 description:"snap_masked-LSalAtl2s144-processed-gene-3.3")

HSP 1 Score: 66.6254 bits (161), Expect = 6.916e-12
Identity = 50/190 (26.32%), Postives = 89/190 (46.84%), Query Frame = 0

Query:  363 DFILAGLDTTGASLGFLFYHLAANPEKQYILRQEI--LSHPDHITEKSLNQMRYLKACLKESARLTPITPF---HVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWL-RGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRL---EWVGSADPMETLWKFTNVP 543
            DF  AG +T+  +L +   +++  PE Q    +EI  +   +  +++    M Y  A L E  R+  + P    HV I  +D  + G+ IP  T    + +    +PK+      F+PER++  G  +++  F   PFG G R+C+G+  A  ++ +  + +LQ  ++   E  G  DP       T +P
Sbjct:  285 DFFQAGGETSATTLLWSVLYISLYPEAQAKCVEEIEAVLGKEDPSQEVYKDMVYCNATLMEIQRMASVGPLTLPHVTI--KDLFIDGYCIPRGTEIYGNIIAFHNNPKFFPSPKSFRPERFIENGKLKKLEAFA--PFGVGKRICMGESMAKNQLFIFFVMMLQKLKISVSENHGVPDPEAYTMGITKIP 470          

BLAST of cytochrome p450 cyp12a2-like vs. SwissProt
Match: gi|1706084|sp|Q09128.1|CP24A_RAT (RecName: Full=1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; Short=24-OHase; Short=Vitamin D(3) 24-hydroxylase; AltName: Full=Cytochrome P450 24A1; AltName: Full=Cytochrome P450-CC24; Flags: Precursor)

HSP 1 Score: 168.703 bits (426), Expect = 9.464e-45
Identity = 134/493 (27.18%), Postives = 223/493 (45.23%), Query Frame = 0

Query:   59 TTSTLRPFEAIPAVPGAWPVVGNFFRLGEKPHGWPRLWSNITRLQSQYDTEGVGLLRMSAPALNRAGQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPSCYAYGFEGSCAIALDQRMGLFQAHPDPEMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAA-NLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILS-HPDHITEKS--LNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWVGSADPMETLWKFTNVPSLKL 547
            T    R   ++P  P  WP++G+   +          W     L+ Q+DT      +       + G    V L  P  +   Y+ E  +P R +    ++ W+  R D   E  G+++ EG  W  +RS  Q+ +M+P         +  + +DF+E   R      +   + DL      + FE  C +  ++R GL Q   + E L  +  ++ ++    +++V      L   L+    Q      D I   VK  ID   Q    +       +I +   L K  L         +    +  LA ++TT  SL ++ Y+L+ NP+ Q  L QE+ S  PD+ T ++  L  M YLKACLKES RLTP  PF  R   +   L  + +P  T+   ++ ++        DS +F+PERWL+  E+++NPF  LPFG G RMC+G+R A+L++ + +  ++Q + +    + +P+E L     VPS +L
Sbjct:   48 TDGETRNVTSLPG-PTNWPLLGSLLEI---------FWKG--GLKKQHDTLAEYHKKYGQIFRMKLGSFDSVHLGSPSLLEALYRTESAHPQRLE----IKPWKAYR-DHRNEAYGLMILEGQEWQRVRSAFQKKLMKPVEIMKLDKKINEVLADFLE---RMDELCDERGRIPDLYSELNKWSFESICLVLYEKRFGLLQKETEEEALTFITAIKTMMSTFGKMMVTPV--ELHKRLNTKVWQAHTLAWDTIFKSVKPCIDNRLQRYSQQPGADFLCDIYQQDHLSKKEL---------YAAVTELQLAAVETTANSLMWILYNLSRNPQAQRRLLQEVQSVLPDNQTPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKPTVLGEYALPKGTVLTLNTQVLGSSEDNFEDSHKFRPERWLQ-KEKKINPFAHLPFGIGKRMCIGRRLAELQLHLALCWIIQKYDIVATDN-EPVEMLHLGILVPSREL 507          

BLAST of cytochrome p450 cyp12a2-like vs. SwissProt
Match: gi|3023553|sp|P97720.1|C11B1_MESAU (RecName: Full=Cytochrome P450 11B1, mitochondrial; AltName: Full=CYPXIB1; AltName: Full=Cytochrome P450C11; AltName: Full=Steroid 11-beta-hydroxylase; Flags: Precursor)

HSP 1 Score: 163.31 bits (412), Expect = 5.729e-43
Identity = 127/493 (25.76%), Postives = 221/493 (44.83%), Query Frame = 0

Query:   61 STLRPFEAIPAVPGAWPVVGNFFRLGEKPHGWPRLWSNITRLQSQYDTEGVGL-----LRMSAPALNRA-GQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPSCYAYGFEGSCAIALDQRMGLFQAHPDPEMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILSHPDHIT---EKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWVGSADPMETLWKFTNVPS 544
            +TLRPFEAIP           + R           W  + ++  +   EG+ L      R   P    + G+ +VV +  P+     +Q +   P R     +L  W   R +  G + GV L  GP W   R  +   M+ P++   ++  V  +A DF+EFL ++  ++  G+   +   S + Y  E S  +   +R+GL     +   L+ +  +  I++  P+L++       W S    +++   + D + ++V   +    Q +++    S        SV+ +L+ +           S +     +DTT   L    + LA NP+ Q  +RQE L+    +    +++++ +  L+A LKE+ RL P+  F  RI   D  L+ + +PA T+   S   M R+P      + + P+RWL    ER   F  L FG G R C+GKR A +EM +L+  VL++FR+E     D +  +++F   PS
Sbjct:   33 NTLRPFEAIP----------QYSR---------NRWLKMLQILREEGQEGLHLEMHEAFRELGPIFRYSMGRTQVVYVMLPEVAEKVFQADSTQPSR----TLLEPWVAHR-EHRGLSRGVFLLNGPEWRFNRLRINPHMLSPKAVQKFVPMVDMVARDFLEFLKKKVLANAHGSLSMNFYSSMFNYTIEASHFVLFGERLGLLGDDLNSGSLKFVNALNSIMKTTPQLMLLPSGLTRWISTRV-WKENFDSWDFVSEYVTKNVKNVYQEVQSGGPQS-------WSVISQLVAEGALTMDAILANSLELTAGSVDTTSVPLVMTLFELARNPDVQQAVRQESLAAEASVAANPQRAMSDLPLLRAVLKETLRLYPVAVFLERILSSDLVLQNYHVPAGTILHMSLYSMGRNPAVFPRPEHYLPQRWL----ERNGSFQHLTFGFGVRQCLGKRLAQVEMLLLLHHVLKSFRVETQERED-VRMVYRFVLAPS 488          

BLAST of cytochrome p450 cyp12a2-like vs. SwissProt
Match: gi|117261|sp|P10612.1|CP11A_PIG (RecName: Full=Cholesterol side-chain cleavage enzyme, mitochondrial; AltName: Full=CYPXIA1; AltName: Full=Cholesterol desmolase; AltName: Full=Cytochrome P450 11A1; AltName: Full=Cytochrome P450(scc); Flags: Precursor)

HSP 1 Score: 158.303 bits (399), Expect = 5.918e-41
Identity = 131/494 (26.52%), Postives = 228/494 (46.15%), Query Frame = 0

Query:   59 TTSTLRPFEAIPAVPGAWPVVGNFFRLGEKPHGWP---RLWSNITRLQSQYDTEGVGLLRMSAPAL-NRAGQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPSCYAYGFEGSCAIALDQRMGLFQAHPDPEMLEQLARV-RVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILS---HPDHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWVGSADPMETLWKFTNVPS 544
            +T T RPF  IP+ PG               +GW    R W      +  Y    V   +   P    + G    V + DP+DV + ++ EGP P+R      +  W  +    + +  GVLL++   W + R ++  ++M P++   ++  + T++ DFV  LHR+ +    G    D+    + + FE    +   +R+G+ +   DPE  + +  V ++    +P L +    +RL+   +  +R   A  D I +  +         ++ K + +         +L +L+  +   S        + +  G+DTT  +L +  Y +A +   Q +LR+E+L+        T K L  +  LKA +KE+ RL PI+    R    D  L+ + IPA TL   +   M RDP + S+  +F P RWL G E  +  F +L FG G R CVG+R A+LEM + +I +L+NF++E    +D ++T++    +P 
Sbjct:   41 STKTPRPFSEIPS-PGD--------------NGWINLYRFWKEKGTQKIHY--HHVQNFQKYGPIYREKLGNLESVYIIDPEDVALLFKFEGPNPERYN----IPPW-VAYHQHYQKPVGVLLKKSGAWKKDRLVLNTEVMAPEAIKNFIPLLDTVSQDFVGVLHRRIKQQGSGKFSGDIREDLFRFAFESITNVIFGERLGMLEEIVDPEAQKFIDAVYQMFHTSVPMLNLPPDLFRLF--RTKTWRDHVAAWDTIFNKAEKYTQNFYWDLRRKREFN-----NYPGILYRLLGNDKLLSEDVKANVTEMLAGGVDTTSMTLQWHLYEMARSLNVQEMLREEVLNARRQAQGDTSKMLQLVPLLKASIKETLRLHPISVTLQRYLVNDLVLRDYMIPAKTLVQVAVYAMGRDPAFFSNPGQFDPTRWL-GKERDLIHFRNLGFGWGVRQCVGRRIAELEMTLFLIHILENFKVELQHFSD-VDTIFNLILMPD 503          

BLAST of cytochrome p450 cyp12a2-like vs. SwissProt
Match: gi|2493375|sp|Q29552.1|C11B1_PIG (RecName: Full=Cytochrome P450 11B1, mitochondrial; AltName: Full=CYPXIB1; AltName: Full=Cytochrome P450C11; AltName: Full=Steroid 11-beta-hydroxylase; Flags: Precursor)

HSP 1 Score: 156.377 bits (394), Expect = 1.921e-40
Identity = 133/486 (27.37%), Postives = 215/486 (44.24%), Query Frame = 0

Query:   65 PFEAIPAVPG-AWPVVGNFFRLGEKPHGWPRLWSNITRLQSQYDTEGVGLLRMSAPALNRAGQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPSCYAYGFEGSCAIALDQRMGLFQAHPDPEMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILSHPDHITE---KSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWL--RGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWVGSADPMETLWKFTNVPS 544
            PFEAIP  PG  W  V   +R      G+    +N   +   +   G  + R      N      +V++  P+DV    + EG +P R    +V   W   R  + G   GV L  GP W   R  +   ++  Q+   +   V  +A DF + L  +   +  G+   D+ PS + Y  E S  +   +R+GL    P+PE L+ +  + V+ +   +L+        W S +  +++     D I  +    I +  Q +      +       + V+ +L+             S D     +DTT   L    + LA NPE Q  LRQE L+    I+E   K++ ++  L+A LKE+ RL P+  F  R    D  L+ + IPA TL       + R+P   +  + + P+RWL  +GS  R   F  L FG G R C+G+R A +EM +L+  VL+NF +E +   D ++ +++F   PS
Sbjct:   37 PFEAIPQFPGKKWMRVLQLWR----EQGFE---NNHLEMHQTFQELG-PIFRFDVGGRN------MVLVMLPEDVERCQKVEGLHPQR----DVPGPWLAYR-HLRGHKCGVFLLNGPTWRLDRLQLNPGVLSLQAMQKFTPLVDGVARDFSQALRARVMQNARGSLTLDIKPSIFRYTIEASNLVLFGERLGLLAHQPNPESLDFIHALEVMFKSTVQLMFMPRSLSRWTS-TGTWKEHFEAWDCIFQYANKAIQRLYQEL------TLGHPWHYSGVVAELLTHANMTVDAIKANSIDLTAGSVDTTAYPLLMTLFELARNPEVQQALRQESLAAAARISENPQKAITELPLLRAALKETLRLYPVGIFLDRCVTSDLVLQNYHIPAGTLVKVLLYSLGRNPAVFARPERYHPQRWLDNQGSGTR---FPHLAFGFGMRQCLGRRLAQVEMLLLLHHVLKNFLVETLVQED-IKMIYRFIMTPS 492          

BLAST of cytochrome p450 cyp12a2-like vs. SwissProt
Match: gi|1148367383|sp|A8WGA0.2|C27C1_DANRE (RecName: Full=Cytochrome P450 27C1; AltName: Full=All-trans retinol 3,4-desaturase)

HSP 1 Score: 157.147 bits (396), Expect = 2.198e-40
Identity = 107/413 (25.91%), Postives = 201/413 (48.67%), Query Frame = 0

Query:  122 NRAGQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPSCYAYGFEGSCAIALDQRMGLFQAHPDPEMLEQLARVRVIIEKLPELVVGS--PWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILSHPDHI------TEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLR-GSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLE 525
            +R G   VV ++D   V    + E   P RG     +  W++ R D+ G +TG++  EG  W ++RS+++Q +MRP+    +   V  + +D V+ +   R    D  TV ++    + Y  EG   I  + R+G  +        E +  + ++       +     P W L P +   + +  ++ D +  F +  +D+    IK + + S+  + G   +L  +++    N    +    + +LAG+DTT  +L +  Y LA +P     ++Q+I    D +      T + +  +  ++  +KE+ RL P+ P + R+  +D  + G+ IP  T         S D +     +EF+P+RW+R  + +R++ F S+PFG+G R C+G+R A+LEM + + ++LQNF +E
Sbjct:  111 SRFGPQFVVSIADRDMVAQVLRSESATPQRGN----MESWKEYR-DLRGRSTGLISAEGDEWLKMRSVLRQLIMRPRDVAVFSSDVNDVVADLVKRVKTLRSQQDDSQTVLNINDLFFKYAMEGVATILYETRLGCLENEIPKMSQEYITALHLMFSSFKTTMYAGAIPKW-LRPIIPKPWEEFCSSWDGLFKFSQIHVDKRLSEIKKQMEKSEEIKGG---LLTHMLVTREMNLEEIYANMTEMLLAGVDTTSFTLSWSTYLLARHP----TIQQQIFEEVDRVLGGRVPTGEDVPYLPLIRGLVKETLRLFPVLPGNGRVTHDDLIVGGYLIPKGTQLALCHYSTSMDEENFPRPEEFRPDRWIRKDASDRVDNFGSIPFGYGIRSCIGRRIAELEMHLALTQLLQNFHIE 510          

BLAST of cytochrome p450 cyp12a2-like vs. nr
Match: gi|780633505|ref|XP_011686066.1| (PREDICTED: cytochrome P450 CYP12A2-like [Wasmannia auropunctata] >gi|780633510|ref|XP_011686067.1| PREDICTED: cytochrome P450 CYP12A2-like [Wasmannia auropunctata] >gi|780633515|ref|XP_011686068.1| PREDICTED: cytochrome P450 CYP12A2-like [Wasmannia auropunctata])

HSP 1 Score: 238.81 bits (608), Expect = 4.284e-68
Identity = 162/511 (31.70%), Postives = 268/511 (52.45%), Query Frame = 0

Query:   64 RPFEAIPAVPGAWPVVGNFFR----LGEKPHGWPRLWSNITRLQSQYDTEGVGLLRMSAPALNRAGQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPSCY-----AYGFEGSCAIALDQRMGLFQAH--PDPEMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEILS-HPDH---ITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEE-----RMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWVGSADPMETLWKFTNVPSLKLDFKLHEL 554
            RP + IP  P A P++GN+FR    +GE           +T+L+  +D  G  ++++      R+    ++ L  P+     Y+ EG +P R  + E L  +R++R  ++    G+   +G  WH+ RS V   MM+P++   ++  +  +  DFV    ++ R+  D  T++  LP  +      +  E  C+IALD R+G   ++   D E    +  VR + + +  + V    W+++ +   N ++    LD +       I++A+   +T  D+S N  +   S+LEKL+  +   + V   M+ D + AG+DTTG     L Y++A NPEKQ  LR+E +S  PD    +T   LNQ RY KAC+KE  RL PI   ++R  Q D  + G++IPA         L+++ P   S ++E+ PERWLRG+ E       +PF  +PFG GPR C+G+RFA++E++ L++ V++NFR+EW     P+E   +F N  +  L FKL +L
Sbjct:   48 RPTKDIPG-PKALPLLGNWFRFLPYIGEYSKA-----DAVTQLRMMHDQYG-DIVKLDQIGFRRS----LIFLFSPELCEKMYRMEGTWPQR-IAMETLHYYRKNREHIYNGQYGLATSQGKIWHDFRSKVNPYMMQPRTVKAHVAQISEVTGDFV----KKMRALRDSETLE--LPKGFNNELLKWALESICSIALDCRLGCLDSNLAADSEPQIMINCVREMFDLMYSMEVQLSLWKVYNT--RNLKKFFRALDTLNGIATKHIERAKAKYET-MDDSTN--LHNRSILEKLLRIDKQTAQV---MALDMLTAGVDTTGNVSASLLYYIANNPEKQEKLREEAISVLPDKTSPVTPDILNQTRYSKACIKEVLRLFPIAVGNLRTMQTDVCIGGYKIPAGFDVAACHSLIAQKPTQFSRTEEYIPERWLRGNTEFPSAKEAHPFAYMPFGFGPRTCIGRRFAEMELETLLLTVIRNFRIEW--HHGPLEIESRFINNVTTPLQFKLIDL 530          

BLAST of cytochrome p450 cyp12a2-like vs. nr
Match: gi|1233180241|ref|XP_022191626.1| (probable cytochrome P450 49a1 [Nilaparvata lugens] >gi|1233180243|ref|XP_022191627.1| probable cytochrome P450 49a1 [Nilaparvata lugens])

HSP 1 Score: 237.269 bits (604), Expect = 1.349e-67
Identity = 147/476 (30.88%), Postives = 253/476 (53.15%), Query Frame = 0

Query:   63 LRPFEAIPAVPGAWPVVGNFFRL--GEKPHGWPRLWSNITRLQSQYDTEGVGLLRMSAPALNRAGQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQS-RPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSS---PDGATVQDLLPSCYAYGFEGSCAIALDQRMGLF--QAHPDPEMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEI---LSHPDHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRGSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWV 527
            ++ +  +P  P   PV+GN +R       +   RL + + RL  +Y      ++++S    N  G+  +V L DP+D+   ++ E   P R  S   L  ++   R D FGE  GV+   G  W++ R  VQQ +M+P++A  Y+  ++  A  FVE +   R  +   PD     D L   + +G E    +AL+ R+G F  +   D +  + +  +      +  L +  P+WRL+ + + N  +    LD I    +  I+Q+ Q I+ K      D    +SVLE+++  N  N+ V   ++ D  L G+DTT A++  + Y L+ N +KQ IL +EI   +     +T K L++M+YL+AC+KE+ R+ P+   + R+   D  L G+ IP     ++   ++S   KY +++ +F+PERWL+   E  + F SLPFG G RMC+G+RFADLE++ L++K+L+++R+E++
Sbjct:   50 VKSYSEVPG-PKPIPVLGNSWRFLPFVGTYEISRLDTVLWRLHREYG----DIVKIS----NMLGRPDMVFLFDPRDIETIFRSEEKLPHR-PSMPSLNYYKHVMRKDYFGEDAGVIAVHGENWYKFRRKVQQPLMQPKTAQLYIPSIEESADTFVERIRNIRDQNLEVPD-----DFLNEIHKWGLESIAKVALNVRLGCFDDKMADDSDTQQLIDAIDTFFSNVGNLELKIPFWRLFNTPTWN--KYVNALDTIMRITQKYINQSMQDIEAKGVECTAD----SSVLERVLAFNDNNTKVAVTLALDMFLVGIDTTSAAVASILYQLSQNQDKQEILVKEIDKVVPGDAKLTSKMLDEMKYLRACIKETLRMYPVVIGNGRLVTNDIVLGGYLIPKGVQVVFQHYVISNSDKYFANAVKFEPERWLKARSEAHHAFASLPFGFGRRMCLGRRFADLELETLLVKILKSYRIEYL 504          

BLAST of cytochrome p450 cyp12a2-like vs. nr
Match: gi|954547165|ref|XP_014616114.1| (PREDICTED: probable cytochrome P450 12b2, mitochondrial isoform X2 [Polistes canadensis])

HSP 1 Score: 236.884 bits (603), Expect = 1.500e-67
Identity = 163/519 (31.41%), Postives = 268/519 (51.64%), Query Frame = 0

Query:   58 STTSTLRPFEAIPAVPGAWPVVGNFFRL-------GEKPHGWPRLWSNITRLQSQYDTEGVGLLRMSAPALNRAGQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPS----CYAYGFEGSCAIALDQRMGLFQAH--PDPEMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEI-LSHPDH---ITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRG-----SEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWVGSADPMETLWKFTNVPSLKLDFKLHEL 554
            +   T+R +E IP     W ++GN FR        G KP     L   +  L+ +Y      +++M         Q + + +   + +   Y+ EGP+P R  + E L  +R++R  ++  T G+   +G  W+  R+ V Q MM+P+    +++ +  IA++F+E + ++ R   D  T++ L PS       +  E  CAIAL+ R+G  +++   D E  + +  +  + + +  L +    WR++     N R +    +++       I    QA K +  N       E SVL+KL+     +  +  VM+ D + AG+DTT  + G L YHLA NP  Q  L++EI L+ PD    IT +++  + YLKACLKES RL+PI    +R  Q+D  + G++IP N   I    L+S DPK   + +EF PERW+RG     S +  +PF  +PFG G R C+G+RFA+ E+++L++K+LQNFR+EW     P++   +  N  +  L FKL +L
Sbjct:   22 NDVETIRSYEDIPGPKPIW-LLGNTFRFLPFIGEFGNKP-----LVVQLKMLRDEYG----DIVKMDG----LPNQRKTIFVFSSEIIEKIYRTEGPWPQR-IAMEALHYYRKNRDWIYKGTYGLTTSQGKDWYNFRTKVNQHMMQPRVINPHIEQMTDIATEFIEKIRKELR---DPVTLE-LSPSFNNEMNKWALESICAIALNHRLGCLKSNLAEDSEPQKMINSIHEMFDLMFRLELMPSLWRIY-----NTRTLKKFFNVLDTLNGISIKYITQA-KERLYNETAANTRERSVLDKLLF---IDEQIACVMALDMLTAGVDTTSNTCGMLLYHLAMNPRAQEKLQEEIKLALPDKELPITVQTMKNISYLKACLKESLRLSPIAIGTLRTMQDDIIIGGYKIPKNCDIILGHSLLSIDPKEFENPNEFIPERWIRGEPNFPSAKNAHPFSYMPFGFGARTCIGRRFAEFEIEILVLKLLQNFRIEW--HRPPIKLNSRLINTIASPLIFKLIDL 510          

BLAST of cytochrome p450 cyp12a2-like vs. nr
Match: gi|970879332|ref|XP_015120970.1| (PREDICTED: cytochrome P450 CYP12A2-like [Diachasma alloeum])

HSP 1 Score: 236.884 bits (603), Expect = 2.273e-67
Identity = 163/516 (31.59%), Postives = 257/516 (49.81%), Query Frame = 0

Query:   65 PFEAIPAVPGAWPVVGNFFR----LGEKPHGWPRLWSNITRLQSQYDT----EGVGLLRMSAPALNRAGQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPSCY-----AYGFEGSCAIALDQRMGLFQAH--PDPEMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEI----LSHPDHITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLR-------GSEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWVGSADPMETLWKFTNVPSLKLDFKLHEL 554
            P+E IP  P + P++GN  R    +GE  +     +  +  LQ  Y      EG+             G+   V L + +D    Y+ EGP P R  + + +  +RQ    ++ +  G++  +G  WH  R  V Q MM P+S   ++  V  +ASDF+  + + R    D  T++  LP+ +      +  E  CAIALD R+G  + +  PD +    +  +  + E   +L V    WRL+ +     RQ+   LD+I       ID+A+  I+        DE    SVLE L+     +    ++M+ D + AG+DTTG + G L YH+A N   Q  LR E+          +T   LN + YLKAC+KES RL PI   ++R  Q+D  + G+QIP     I S  +++  P++ S   EF PERWLR        S +  +PF  +PFG GPR C+G+RFA++E++ L++++++NFR+EW  S  P++    F N  +  L FKL ++
Sbjct:   46 PYENIPG-PKSLPLIGNMIRFMPFIGE--YANLSAFDQMLMLQRDYGNIVKLEGI------------PGRRAAVFLFNAEDCEKMYRLEGPRPMR-VAIQTMASYRQRNSHLYKDKYGLVASQGEAWHNFRKNVNQHMMSPRSIKPHVVQVDEVASDFIARIRKLR----DVKTLK--LPTTFNNEMNKWALESICAIALDHRLGCLEDNLPPDSDPQRMINAIHKMFELFYQLEVLPSLWRLYDT--KKLRQLFRTLDLINGIACKHIDEAQ--IRFSKSPKGPDE---RSVLESLL---SIDEQTAYIMALDMLTAGIDTTGNATGTLLYHIAINHRVQEKLRAEVENLLTEKISPVTYDILNNIPYLKACIKESLRLIPIAIGNLRTMQKDVVIGGYQIPKGCDLIASHSVLAVSPEHFSQPGEFIPERWLRENTLATIKSAKEAHPFAYMPFGFGPRTCIGRRFAEMEIETLVMRIVKNFRVEW--SNPPIQINSGFINTIASPLQFKLTDI 527          

BLAST of cytochrome p450 cyp12a2-like vs. nr
Match: gi|954547161|ref|XP_014616112.1| (PREDICTED: probable cytochrome P450 12b2, mitochondrial isoform X1 [Polistes canadensis] >gi|954547163|ref|XP_014616113.1| PREDICTED: probable cytochrome P450 12b2, mitochondrial isoform X1 [Polistes canadensis])

HSP 1 Score: 236.498 bits (602), Expect = 2.472e-67
Identity = 163/519 (31.41%), Postives = 268/519 (51.64%), Query Frame = 0

Query:   58 STTSTLRPFEAIPAVPGAWPVVGNFFRL-------GEKPHGWPRLWSNITRLQSQYDTEGVGLLRMSAPALNRAGQGRVVILSDPQDVPIAYQHEGPYPDRGQSFEVLRRWRQSRPDMFGETTGVLLEEGPRWHEIRSMVQQDMMRPQSAFFYLDPVQTIASDFVEFLHRQRRSSPDGATVQDLLPS----CYAYGFEGSCAIALDQRMGLFQAH--PDPEMLEQLARVRVIIEKLPELVVGSPWWRLWPSLSANYRQVAANLDIIGDFVKSKIDQARQAIKTKADNSKNDEIGETSVLEKLILKNGWNSSVPFVMSFDFILAGLDTTGASLGFLFYHLAANPEKQYILRQEI-LSHPDH---ITEKSLNQMRYLKACLKESARLTPITPFHVRIFQEDFSLKGFQIPANTLGIWSSLLMSRDPKYVSDSDEFQPERWLRG-----SEERMNPFVSLPFGHGPRMCVGKRFADLEMQVLMIKVLQNFRLEWVGSADPMETLWKFTNVPSLKLDFKLHEL 554
            +   T+R +E IP     W ++GN FR        G KP     L   +  L+ +Y      +++M         Q + + +   + +   Y+ EGP+P R  + E L  +R++R  ++  T G+   +G  W+  R+ V Q MM+P+    +++ +  IA++F+E + ++ R   D  T++ L PS       +  E  CAIAL+ R+G  +++   D E  + +  +  + + +  L +    WR++     N R +    +++       I    QA K +  N       E SVL+KL+     +  +  VM+ D + AG+DTT  + G L YHLA NP  Q  L++EI L+ PD    IT +++  + YLKACLKES RL+PI    +R  Q+D  + G++IP N   I    L+S DPK   + +EF PERW+RG     S +  +PF  +PFG G R C+G+RFA+ E+++L++K+LQNFR+EW     P++   +  N  +  L FKL +L
Sbjct:   35 NDVETIRSYEDIPGPKPIW-LLGNTFRFLPFIGEFGNKP-----LVVQLKMLRDEYG----DIVKMDG----LPNQRKTIFVFSSEIIEKIYRTEGPWPQR-IAMEALHYYRKNRDWIYKGTYGLTTSQGKDWYNFRTKVNQHMMQPRVINPHIEQMTDIATEFIEKIRKELR---DPVTLE-LSPSFNNEMNKWALESICAIALNHRLGCLKSNLAEDSEPQKMINSIHEMFDLMFRLELMPSLWRIY-----NTRTLKKFFNVLDTLNGISIKYITQA-KERLYNETAANTRERSVLDKLLF---IDEQIACVMALDMLTAGVDTTSNTCGMLLYHLAMNPRAQEKLQEEIKLALPDKELPITVQTMKNISYLKACLKESLRLSPIAIGTLRTMQDDIIIGGYKIPKNCDIILGHSLLSIDPKEFENPNEFIPERWIRGEPNFPSAKNAHPFSYMPFGFGARTCIGRRFAEFEIEILVLKLLQNFRIEW--HRPPIKLNSRLINTIASPLIFKLIDL 523