BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 766.148 bits (1977), Expect = 0.000e+0
Identity = 364/607 (59.97%), Postives = 465/607 (76.61%), Query Frame = 0

Query:   12 MRNLTTP----ANMSRRGSSSSVGPPPLASSHQGRYKGLLSTPGVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKA-GGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEPLSTEEISKQTEPEWKLAP 597
            MRNL TP    A+ SRRGSS  +    L+S +Q RYKGL+ST G+VKFSAWKRE +I +   HEF+L KI EK+P+++W+KD+++GR E +G V S V+K+ASGLTK GF K DV+ + CSN+VEYW++AL  W CGG VMPVNCE+  D LE Q+ E+K K++VCDDFNY D LD  ENT +++ +I+IG E  A    I M DL+ DDGK  P ++   W +DV ++PFTS SSG + GI+HT+KSLM+ F+SPDGAANH+ DQ+IGDSI C N+FFHM+GFY  AL SIYG+S+Y+LSE+S+  F+E +VD KT + + Y WQIR+LSQS  +  YDLS LK +++  G+LSSTIRME+L+R P++KYVREAYGLNECGLVTLTYP+EKKNSIA++K  + P+DH+IP+GLPNMY+ +KII+RQ    V+GPD+QGEIC+KS Q  +GYLN++    FD++ +FHTGDLGYYD+QG++Y++EKI NLI FWMYEVAP++LESRLLGSNNIVDAAVVGIP+KENG                EENLTNLMESRLQDHERIRGGLYF+QNIPRDENWKVLR VL  Y+P     I +  +   +L P
Sbjct:    1 MRNLATPSFSGASNSRRGSSVGL-VSMLSSPNQARYKGLISTAGIVKFSAWKREXEIQKLNFHEFILQKIGEKEPDRIWIKDLSIGRMERFGNVHSLVNKVASGLTKNGFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVCDDFNYVDGLDFLENTNMIEKLIIIGNECTAQDKVILMTDLLNDDGKKVPQKVIFDWAKDVIYMPFTSTSSG-AKGILHTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYGISIYTLSEFSEQNFLEALVDXKTASISCYAWQIRILSQSPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRIPSIKYVREAYGLNECGLVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYLNKEESSAFDNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLRSVLAEYKPHVNNLIKRPNKLFKQLHP 605          

HSP 2 Score: 64.3142 bits (155), Expect = 7.824e-11
Identity = 31/94 (32.98%), Postives = 54/94 (57.45%), Query Frame = 0

Query:  748 LSYWLKVYINPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLMCNV 841
            + +W+   + P++LE  ++    + D +V        G +PRAFVVLK G+E T E + +   +RL D + +RGG+ F   +P+D N K++ +V
Sbjct:  489 IQFWM-YEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLRSV 581          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 155.992 bits (393), Expect = 3.598e-40
Identity = 134/568 (23.59%), Postives = 250/568 (44.01%), Query Frame = 0

Query:   36 ASSHQGRYKGLLSTPG---VVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDL-IEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGD------SIACGNWFFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDNKRLFD---SEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG---------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQ 575
            A      +   L  PG   ++++S ++ E  +P     +F+   +++    +  +  +T GRT TY  V S   K  S L +LG  K DV+ +   N  E+ +  +     G  +  +N     + +  Q+  + AK ++      +++    E    ++ +IVIG E     C    ++ I DDG     R  +   +D+  LP++S ++G   G+  TH +L+         + H     IGD      SIA   +F   +   +++    +GV + +L ++    F+  +V  K     + P  +  ++ S  I    LS +K +         T+  + + +  +    +E YG+ EC  VT   PK+         ++     ++IP       T  KII+  + + V GP E GE+C+   Q   GY    NK+  D     G+ HTGD+ YY+  G  Y +++++ LI     +VAP+ LE  +     + D A VG+PD+ NG               E+ + + +  ++  H+++  G+ F+  +P+++  K+LR  L+
Sbjct:   27 AKQSXSHFGAPLIKPGEEKLIQYSPYE-EVVLPSIPYADFIWENLEDYKEREALVCGMT-GRTYTYEMVHSLSQKFGSALLRLGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQKIIVIGMEETPSDCKSFMEMAIFDDGSFFDQRETIDPHKDILALPYSSGTTGPPKGVALTHSNLVNNITQLSHPSIH---NFIGDEVEQHISIAVLPFFHIFAMTCSLSAGLRFGVKLITLPKFEPEMFINSLVQYKPTHMLVVPPLVSFMTSSPLIKPSLLSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQEGYGMTECSPVTHVQPKDG--------IIYGSCGNLIP------NTIGKIIDIDTGK-VLGPGENGELCVSGPQIMQGYYK--NKKATDKIIQNGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVVRKNRSVMEQGIIDFVTEKVAPHKKLGAGVMFVDTLPKNQTGKILRRELK 572          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000002083 (supercontig:LSalAtl2s:LSalAtl2s138:399496:447452:1 gene:EMLSAG00000002083 transcript:EMLSAT00000002083 description:"maker-LSalAtl2s138-snap-gene-4.7")

HSP 1 Score: 118.627 bits (296), Expect = 1.239e-30
Identity = 68/189 (35.98%), Postives = 103/189 (54.50%), Query Frame = 0

Query:  668 RNRSRRGSMDCILEDKPSALGATGGRKGSQAATSGKRRSSETRRVSAGGTRGGGGSRRGSGSEIPIVPLTGSASSAVEGGLSYWLKVYINPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLMCNVEKLSQEAHVVDTHFI 856
            R + RR SMD I             R+G  +   G     ++RR+S+       GSRRGS   +  +    S+    +  L+YWL++ IN   LE  + SHPAV+D  V+  T+ G+G LPR +V LK GY+ ++E+IL   +A++ DT+ LRGG+V  DK+ +D NGK++ N+EK  +     D   I
Sbjct:   27 REKDRRSSMDRI-------------REGEVSVNVGSSAPGKSRRMSSAR-----GSRRGS--XVDALSSLESSHDMGQNNLTYWLQITINTEALEEYLKSHPAVEDAVVRGVTIEGIGELPRGYVSLKQGYDTSSEDILSNMNAKVLDTEQLRGGLVIIDKIGRDMNGKVIMNLEKYDKNVVGTDNDLI 195          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 71.2478 bits (173), Expect = 6.283e-13
Identity = 111/501 (22.16%), Postives = 204/501 (40.72%), Query Frame = 0

Query:  105 VDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWD-EDVAFLPFTSASSGCSAGIMHTHKSLM-AWFFSPDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAI----------IKY--DLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQN-FIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSN-NIVDAAVV-----------GIPDKENG----EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRD 572
            V K A  L + G  K DV+ +   N  + ++   A   C G V      I  + L  +V E +AK ++C +  Y  V D++         I+I  + +    +     I+   +T  P   + +D + +A + +TS ++G   GI     SL    F SP    N          I C   F H+SGF      SI    V +    S      L V+    T +  P+   ++S S  +          I+Y  +   +K++  +G      I  +L + F  ++ V   Y + E G + L    ++ N      +                  +V I +  S E + GP+  GE+  ++     +GYLN  ++ K+ F  +G+  +GD+ +Y+  G ++Y  +I+ ++      V P  +E+ +   N NI++A +            GI   + G     E +   +  R+ D++ +   +   + +PR+ N K+LR+
Sbjct:   83 VPKFAKYLYENGLRKGDVVHIIIGNRGDVYIPVHATLLCQGVVSISVDNISTETLLSEVQETQAKFVICTNRTYYKVKDLSS--------IIINLDTQLHAILE----IDMSTQTQIPE-QIEFDLQTLAVILWTSGTTGKPKGIQLNMVSLWNLLFLSPKTDGNML--------ITCN--FAHVSGFNYSCFWSI-NFDVCTFHFISPEDVTSLGVEKIIFTLSQIPFTTIVVSASHIVYIAEKIVNDQIEYSSNFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVGNM------------------EVYIRDIHSGERL-GPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSKTEKIQTILGGIVVIKKGYRIDPEEIRQFVNDRIDDYKHLEIIILTNEPLPRNPNGKLLRN 540          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 70.0922 bits (170), Expect = 1.149e-12
Identity = 79/322 (24.53%), Postives = 141/322 (43.79%), Query Frame = 0

Query:  284 FFHMSGFYTVALASIY-----GVSVYSLSEYSDNAFVELIVDNKTQTANLY----PWQIRLLSQSKAII-KYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIV-DAAVVGIPDKENGEENLT---------------NLMESRLQDHER-----IRGGLYFIQNIPRDENWKVLRDVL 574
            FFH  GF T  L +I      G+ + S +EY D +   L    +T   N+      + +++   S  +  ++DLS +  +   GG +++    +   +FP++K + + YG  E   ++L    +    +                  P    Q++I+N   N    GP+  G+I +K       Y+  D++     +GFF+ GD+GYYDS G I++  + ++L+    + V P  +ES +    + V D  V G  DK NG E L+               N++E   + ++      ++GG+     IPR+ N K+ R  L
Sbjct:  252 FFHPGGF-TEGLRNILPQKRTGIILRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFDLSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTETLSISLGISDQHLGRL-----------------FPKY--QLRILN--PNGEPLGPNSVGDIVVKPGYGMRRYIG-DHEYNITQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDK-NGIERLSGVIVLHHIEKGLSLQNVLERTFEAYKFPRYKWLQGGVRVTSAIPRNANGKIFRGRL 549          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000004507 (supercontig:LSalAtl2s:LSalAtl2s235:163315:165883:-1 gene:EMLSAG00000004507 transcript:EMLSAT00000004507 description:"maker-LSalAtl2s235-augustus-gene-1.27")

HSP 1 Score: 68.1662 bits (165), Expect = 5.595e-12
Identity = 101/465 (21.72%), Postives = 198/465 (42.58%), Query Frame = 0

Query:  155 EFDLLEEQVTEAKAKVIVCDDFNY--------EDVLDMA--ENTELLKHVIVIGTEGK------AGGCIPMND-LIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNWFF-HMSGFYTVALASIYGVSVYSLSEYSD-NAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGL-PNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGY-LNEDNKRLFDS-----EGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGEENLTNLMESRLQD-------------HERIRG-----GLYFIQNIPRDENWKVLRDVLQ 575
            E D L E+    KA VI+  D  Y        ++V+D A  ++++ +KHV+V    GK      +G  + + D +++ + +  P  ++    ED  F  +TS S+G   G++H+    + +          + +  I   +A   W   H    Y   +     V   S   Y D + + +L+ +         P  IR L + +    Y L+  KS + + G +   I +E         +  +  G N C +V   +  E      T +  E   + V  +G+ P +     +++ + NE ++  ++QG +C+K+    +   + +D+KR  ++      G++ TGD  Y   +G I  + +I+++I+   + +    +E  L   +NIV+AAVVG P    GE     ++   L +              + I G        F +++P+  + K++R +L+
Sbjct:  112 EKDELGEREFNCKANVIITVDEGYRGGRVIPLKEVVDEAIKKSSQPIKHVLVAKRSGKGHVKMKSGRDVYLEDAMLQVESECHPESMS---SEDPLFYLYTSGSTGAPKGLVHSTAGYLLYTSFTHKIIFDFCEHDIFGCVADIGWITGHSYIVYGPLMNGGTSVLFESTPTYPDPSRYWKLVEELGITQFYTSPTAIRQLMKYED--SYVLNSDKSSLRTLGSVGEPINIEAW------NWYNDLVGRNRCDIVDTWWQTETGGICITPRPSELNAEIVPALGMRPFLGINPVLLDDKGNE-IKENNKQGSLCLKTPWPGMARSIYDDHKRYQETYFSAFPGYYFTGDGAYRTKEGYISIVGRIDDVINVSGHRLGTAEVEDVLTKHDNIVEAAVVGXPHDIKGEAIYAFVVPKNLDEGVKKFEKGIQKLVKDNISGFAVPEKFQFCEDLPKTRSGKIMRRILR 564          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 61.2326 bits (147), Expect = 6.494e-10
Identity = 43/156 (27.56%), Postives = 77/156 (49.36%), Query Frame = 0

Query:  440 GPDEQGEICIKSAQ-NFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLG-SNNIVDAAVVGI---------------PDKENGEENLTNLMESRLQDHERIRGGLYFIQN-IPRDENWKVLRDVLQ 575
            GP++ GE+  + A     GYLN  E+N+++F+ +G+ HTGD  +Y  QG  ++  +I  +I      V P  LE  L    ++I++  VV +                DK+   E +   +  ++ +++RI G L   +  +PR+   K+LR  +Q
Sbjct:  329 GPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVVPVVEKKQTVLGAIIVVKNDKKINVETIRKELNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEIQ 484          

BLAST of 4-coumarate-- ligase-like vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 53.1434 bits (126), Expect = 2.800e-7
Identity = 61/259 (23.55%), Postives = 115/259 (44.40%), Query Frame = 0

Query:  287 MSGFYTVALASIYGVSV-YSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYD-------LSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGEE 535
            +S F  +  A + G  + + L  +  N +V L       T  L    I L   SK ++  D       L+C+ +   +    + T+ + +LE     K +R  YG  E G V+          IA  K  ++    +  VG P  + + KI+++  N  +   +  GE+ +++     GY  +   +K++   + ++H+GDLG    +G    I ++++++      + P  +E  LL   +IV+  +VG+PD   GEE
Sbjct:   19 LSHFCVINNALLTGNRIGFDLKYHXINLWVPLFHTFGCVTGTL----ISLCYGSKLVLPSDGFDPTACLNCIPAEKCTYLFGTPTMYIAVLE---AQKKLRNIYGATETGPVSF---------IACPK--DSFEVRINTVGPPLGHIEAKIVDQ--NGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEE 257          

HSP 2 Score: 51.9878 bits (123), Expect = 7.375e-7
Identity = 49/211 (23.22%), Postives = 98/211 (46.45%), Query Frame = 0

Query:  329 YPWQIRLLSQ--SKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGEE 535
            YP  ++   +  S  + K+++  +K  I+ GG        +  E+   +K +   YG  E G V+          IA  K  ++    +  VG P  + + KI+++  N  +   +  GE+ +++     GY  +   +K++   + ++H+GDLG    +G    I ++++++      + P  +E  LL   +IV+  +VG+PD   GEE
Sbjct:  289 YPMFVKDFPKTLSGKVKKFEMKAIKLGIT-GGSNCLVEACKDFEKHLNMKSLMNIYGATETGPVSF---------IACPK--DSFEVRINTVGPPLGHIEAKIVDQ--NGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEE 485          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|75296548|sp|Q7XXL2.2|4CLL9_ORYSJ (RecName: Full=4-coumarate--CoA ligase-like 9)

HSP 1 Score: 187.963 bits (476), Expect = 1.071e-49
Identity = 141/539 (26.16%), Postives = 259/539 (48.05%), Query Frame = 0

Query:   69 IHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPP--PRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW-FFHMSGFYTVALASI-YGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLS--CLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPK-------EKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDK----------------ENGEENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            + EF+L   +        ++    GR+ TYG+VA +  + A  L  +G  K  V+ +   N   Y +V+L     G     VN       +++QV +++AK++V ++  ++ V D          VI +G   +  G I  + L+    +T      ++ +   D+  LP++S ++G S G+M +H++L++   S   A      +  G  +  G   FFH+ G   +  A++ +  +V  +  +    F+  +VD++   A L P  +  + +S    ++DLS   LKSV+++   L+  +      +FP V+ V EAYGL E   +TLT+          KK+S+                 LPN+  +VK ++  +  ++   +  GE+C++S     GY    E+ +R  D +G+ HTGD+GY D  G ++ +++I+ LI +  ++VAP  LE+ LL   ++ DAAV G+PD+                E GEE +   +  R+  ++R+R  L+ +  IP+  + K+LR  L++
Sbjct:   35 VPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTARFARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSEAKLVVANEVAFDKVKDAGVP------VIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSDLCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAV---APETAGQVVTLGLMPFFHIYGITGICCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLALKSVMTAAAPLAPDLLAAFQRKFPGVQ-VEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGFI--------------LPNL--EVKFVDPDTGRSLPA-NTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVGYIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVACVVRRHGAEEGEEEIVAYVAERVASYKRVR-VLHIVDAIPKSVSGKILRRQLRD 545          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|75311450|sp|Q9LQ12.1|4CLL1_ARATH (RecName: Full=4-coumarate--CoA ligase-like 1; AltName: Full=4-coumarate--CoA ligase isoform 10; Short=At4CL10)

HSP 1 Score: 185.652 bits (470), Expect = 5.702e-49
Identity = 154/529 (29.11%), Postives = 265/529 (50.09%), Query Frame = 0

Query:   71 EFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW-FFHMSGFYTVALASIYGV-SVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLK--SVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTY-PKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYL--NEDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            EF+L  ++E   N  +++ VT G+  TYG V  +  ++A  LT LG  K  V+ +   N  EY ++AL     GG     N       +++QV  + A+ I+ D  NYE V  +         VIV+G E K  G +   DL+E   K            D+  LPF+S ++G   G+M TH++L+A   S          +MIG  +  G   FFH+ G   +  A++     V ++S Y    F+  ++ ++   A + P  I  L ++  + ++DLS LK  SV+++   L+  +      +FP V+ V+EAYGL E   +TLT+   EK   IA          + +   LPN+  +VK I+  +  ++   +  GE+C++S     GY    E+  +  D +G+ HTGD+GY D  G I+ +++I+ LI +  ++VAP  LE+ LL   ++ D AVV +PD+E G                EE++ N + + +  ++++R  ++F+ +IP+  + K++R +L++
Sbjct:   30 EFVLQGVEEYTENVAFVEAVT-GKAVTYGDVVRDTKRLAKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGARGIITDATNYEKVKSLG------LPVIVLGEE-KIEGAVNWKDLLEAGDKCGDTDNEEILQTDLCALPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVR---SEMIGQIVTLGLIPFFHIYGIVGICCATMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSVMTAAAPLAPELLTAFEAKFPNVQ-VQEAYGLTEHSCITLTHGDPEKGQGIA--------KRNSVGFILPNL--EVKFIDPDTGRSLP-KNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVINPKATEKEEDILNFVAANVAHYKKVR-AVHFVDSIPKSLSGKIMRRLLRD 534          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|41688574|sp|Q27757.2|LUCI_PHOPE (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 182.185 bits (461), Expect = 8.409e-48
Identity = 139/504 (27.58%), Postives = 237/504 (47.02%), Query Frame = 0

Query:  107 KIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCE-IEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIE-------DDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW------FFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIP--VGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            ++A    K G  + D I +C  N ++++L  +A+   G    PV+ + IE +L+   +   K ++I C    ++ VL++    + ++ +I++      GG   +N+ I        D  K  P   N   D+ VA + F+S ++G S G+M THK+++A F       +H  D   G++I           F H  G  T       G  V  +  + +  F++ + D K ++  L P  +    +S  + KYDLS LK + S G  LS  I   + +RF  + +VR+ YGL E                 T+ VL TP+  V P   G    +  VK+++  + + + GP+E GE+  K       Y N  E  K + + +G+  +GD+ YYD+ G  Y ++++++LI +  Y+VAP  +E  LL    IVDA V GIPD+  G                E+ + N + S++   + +RGG+ F+  IP+    K+ R VL+ 
Sbjct:   61 RLAESFKKYGLKQNDTIAVCSENGLQFFLPLIASLYLGIIAAPVSDKYIERELIH-SLGIVKPRIIFCSKNTFQKVLNVKSKLKYVETIIILDLNEDLGGYQCLNNFISQNSDINLDVKKFKPNSFN--RDDQVALVMFSSGTTGVSKGVMLTHKNIVARF-------SHCKDPTFGNAINPTTAILTVIPFHHGFGMTTTLGYFTCGFRVALMHTFEEKLFLQSLQDYKVESTLLVPTLMAFFPKSALVEKYDLSHLKEIASGGAPLSKEIGEMVKKRF-KLNFVRQGYGLTE----------------TTSAVLITPDTDVRPGSTGKIVPFHAVKVVDPTTGK-ILGPNETGELYFKGDMIMKSYYNNEEATKAIINKDGWLRSGDIAYYDNDGHFYIVDRLKSLIKYKGYQVAPAEIEGILLQHPYIVDAGVTGIPDEAAGELPAAGVVVQTGKYLNEQIVQNFVSSQVSTAKWLRGGVKFLDEIPKGSTGKIDRKVLRQ 536          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|74897041|sp|Q54P77.1|4CL1_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 178.718 bits (452), Expect = 1.228e-46
Identity = 146/573 (25.48%), Postives = 253/573 (44.15%), Query Frame = 0

Query:   46 LLSTPGVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVL-DMAENTELLKHVIVIGTEGKA------------GGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMI-------GDSIACGNWFFHMSGFYTVALASI-YGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEP 579
            ++   G   F++      IP + + + +L  I+ K P++V L D    +  +   VA  ++K+A GL KL   K DV+ +   N  EY  +       GG    VN +   + L   +     + +      YE +  D+      ++ VI++   G+             G  +  N LI ++GK  P  + +   +D A +PF+S ++G   G+  +H ++++         N Y  Q I        D++     FFH+ G     +  +  G  V  L ++    F+ELI   K   + + P    + ++S  + K+DLS L+++ S    LSS +   + ERF     +++ YG  E        P     S  +A +L           LPN     KII+ ++ EN+ G  E+GEICIK     +GY N +     + D +GF  TGD+GY D  G  + +++ + LI    ++V P  LE+ LL    + DA VVG+   + G                E+ L +    ++ +++  RGG++FI  IP+    K+LR  L+++ P
Sbjct:    2 IIKVKGQTYFTSKYPNIIIPEKPVPQLILKHIRSK-PDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIAGQTLKEIDQLTLSSDGIVMSFNQLINNNGKDYP-IVRIDPKKDTAIIPFSSGTTGLFKGVCLSHHNIVS---------NTYQTQTIETSTYKKNDTVMGILPFFHIYGLMLFLMLMVKQGHRVVVLPKFEPVRFLELIQKYKVAISFIVPPVAIMFAKSPIVDKFDLSSLRTLFSGAAPLSSEVEDLIKERFKGRLVIKQGYGATELSPACFVIPSGLVKS-GSAGIL-----------LPNQLA--KIISPETGENL-GMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFLKTGDIGYVDEDGYYFIVDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKDFNP 548          

HSP 2 Score: 72.0182 bits (175), Expect = 9.549e-12
Identity = 33/83 (39.76%), Postives = 49/83 (59.04%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE +++SHP V D  V   +   +G +PR FVV+K     T +E+L +AH ++A+  H RGGI F   +PK   GKL+
Sbjct:  457 VPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLL 539          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|74897042|sp|Q54P78.1|4CL2_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 2; Short=4CL 2; AltName: Full=4-coumaroyl-CoA synthase 2)

HSP 1 Score: 177.948 bits (450), Expect = 2.556e-46
Identity = 147/568 (25.88%), Postives = 252/568 (44.37%), Query Frame = 0

Query:   51 GVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVL-DMAENTELLKHVIVIGTEGKA------------GGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMI-------GDSIACGNWFFHMSGFYTVALASI-YGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEP 579
            G + F++      IP + I   +L  I+ K P++V L D    +  +   VA  ++K+A GL KL   K DV+ +   N  EY  +       GG    VN +   + L   +     + +      YE +  D+      ++ VI++   G+             G  +  N LI ++GK  P  + +   +D A +PF+S ++G   G+  +H +L++         N +  Q +        D++     FFH+ G  T  +  +  G  V  L ++    F++LI   K   + + P    + ++S  + K+DLS L+++ S    LS  +   + ERF     +++ YG  E        P     S  +A +L           LPN    VKII+ ++ EN+ G  E+GEICIK     +GY N +     + D +GFF TGD+GY D  G  + +++ + LI    ++V P  LE+ LL    + DA VVG+   + G                E+ L +    ++ +++  RGG++FI  IP+    K+LR  L++  P
Sbjct:    7 GQIYFTSKYPNIIIPEKPIPHLILKHIRSK-PDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIAGQTLKEIGQLTLSSDGIVMSFNQLINNNGKDYP-IVRIDLKKDTAIIPFSSGTTGLFKGVCLSHHNLVS---------NTHQTQTVETTNYKKNDTVMGQLPFFHIYGLMTYLILMVKQGHCVVILPKFEFVRFLDLIQKYKVAISFIVPPIAIMFAKSPIVDKFDLSSLRTLFSGAAPLSREVEDLIKERFKGKLIIKQGYGATELSPACFVIPSGLIKS-GSAGIL-----------LPNQL--VKIISPETGENL-GMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFFKTGDIGYVDEDGYYFIVDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKDINP 548          

HSP 2 Score: 72.0182 bits (175), Expect = 1.106e-11
Identity = 33/83 (39.76%), Postives = 49/83 (59.04%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE +++SHP V D  V   +   +G +PR FVV+K     T +E+L +AH ++A+  H RGGI F   +PK   GKL+
Sbjct:  457 VPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLL 539          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|12229666|sp|Q9S777.1|4CL3_ARATH (RecName: Full=4-coumarate--CoA ligase 3; Short=4CL 3; AltName: Full=4-coumarate--CoA ligase isoform 3; Short=At4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 175.252 bits (443), Expect = 2.621e-45
Identity = 144/544 (26.47%), Postives = 251/544 (46.14%), Query Frame = 0

Query:   55 FSAWKRETDIPRQY-IHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLV---TLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPD------------KENG----EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQ 575
            F +   + DIP    +H +   K+     +K  L   + G++ TYG+      ++ASGL KLG  K DVI +   N  E+    + A   G      N       L +Q+  + AK+I+      + + ++ EN  L     +   E     C+P + LI DD   P         +D A LPF+S ++G   G++ THKSL+          N  +     D I C    FH+    +V L S+  G +V  + ++   A ++LI  ++   A L P  +  L+++  +  YDLS ++ V+S    L   ++  L  R P    + + YG+ E G V   +L + KE   + + +             G      ++K+++ ++  ++ G ++ GEICI+  Q    YLN  E      D EG+ HTGD+GY D    I+ +++++ +I F  ++V P  LES L+  ++I DAAVV   D            + NG    EE++   +  ++  ++R+   ++F+ +IP+  + K+LR  L+
Sbjct:   35 FRSKLPDIDIPNHLPLHTYCFEKLSSVS-DKPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGENLTL-----ITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQA-ILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGS------------CGTVVRNAELKVVHLETRLSL-GYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLH-KVFFVASIPKSPSGKILRKDLK 557          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|61213879|sp|Q26304.1|LUCI_LUCMI (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 174.096 bits (440), Expect = 4.991e-45
Identity = 129/503 (25.65%), Postives = 232/503 (46.12%), Query Frame = 0

Query:  107 KIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDD---GKTPPPRLNL---SWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW------FFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPR----DENWKVLRDVLQ 575
            ++A  +   G    + I +C  N  E+++  LA    G  V P N       L   +  A+  ++         VL++ +    +K ++++ ++   GG   M   I+     G  P   + +   +  + VA L  +S S+G   G+  TH+  +  F       +H  D + G+ ++ G        F H  G +T       G  V  L+++ +  F+  + D K  +  L P    +L++S+ I K+DLS L  + S G  L+  +   +  RF  +  VR+ YGL E     +  P+      A+ KV        +P+       +VK+I+  + + + G + +GEIC+K     +GY N  E  +   D EG+ HTGD+GYYD     + ++++++LI +  Y+V P  LES LL   NI DA V G+PD + G                E+ + + + S++ +H+R+RGG+ F+  +P+      + KV+R++L+
Sbjct:   63 RLAEAMKNFGMKPEEHIALCSENCEEFFIPVLAGLYIGVAVAPTNEIYTLRELNHSLGIAQPTIVFSSRKGLPKVLEVQKTVTCIKKIVILDSKVNFGGHDCMETFIKKHVELGFQPSSFVPIDVKNRKQHVALLMNSSGSTGLPKGVRITHEGAVTRF-------SHAKDPIYGNQVSPGTAILTVVPFHHGFGMFTTLGYFACGYRVVMLTKFDEELFLRTLQDYKCTSVILVPTLFAILNKSELIDKFDLSNLTEIASGGAPLAKEVGEAVARRF-NLPGVRQGYGLTETTSAFIITPEGDDKPGASGKV--------VPL------FKVKVIDLDTKKTL-GVNRRGEICVKGPSLMLGYSNNPEATRETIDEEGWLHTGDIGYYDEDEHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPDAGELPGAVVVMEKGKTMTEKEIVDYVNSQVVNHKRLRGGVRFVDEVPKGLTGKIDAKVIREILK 542          

HSP 2 Score: 60.077 bits (144), Expect = 4.456e-8
Identity = 30/86 (34.88%), Postives = 48/86 (55.81%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLMCNV 841
            + P  LE V++ HP + D  V     P  G LP A VV++ G   T +EI+ + ++++ +   LRGG+ F D++PK   GK+   V
Sbjct:  451 VPPAELESVLLQHPNIFDAGVAGVPDPDAGELPGAVVVMEKGKTMTEKEIVDYVNSQVVNHKRLRGGVRFVDEVPKGLTGKIDAKV 536          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|182627620|sp|Q54P79.2|4CL3_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 172.555 bits (436), Expect = 1.711e-44
Identity = 148/570 (25.96%), Postives = 250/570 (43.86%), Query Frame = 0

Query:   51 GVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVL-DMAENTELLKHVIVIGTEGKA------------GGCIPMNDLIEDDGKT-PPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMI-------GDSIACGNWFFHMSGFYT-VALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNED--NKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEPL 580
            G   F++      IP + I   +L  I+ K P++V L D    +  +   VA  ++K+A GL KL   K DV+ +   N  EY  +       GG    VN +   + L   +     + +      YE +  D+      ++ VI++   G+             G  +  N L  ++GK  P  R++L+  +D A +PF+S ++G   G+  +H +L++         N Y  Q I        DS+     FFH  G    + L    G  + +L ++    F+ELI   K   + + P    + ++S  + K+DLS L+++      L S I   + ERF     +++ YG  E        P     S ++  +L            PN+    KII+ ++ EN+ G  E+GEICIK     +GY N +     + D +GF  TGD+GY D  G  + I++ + LI    ++V P  LE+ LL    + DA VVG+   + G                E+ L +    ++ +++  RGG++FI  IP+    K+LR  L+++  L
Sbjct:    7 GQTYFTSKYPNIIIPEKPIPHLILKHIRYK-PDQVLLVDGLTFKEYSSHFVADTIEKVACGLNKLNIKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIAGQTLKEIDQLTLSSDGIVMSFNQLTNNNGKDYPIVRIDLT--KDTAIIPFSSGTTGLFKGVCLSHYNLVS---------NTYQTQTIETSTYKKNDSVIGVLPFFHSFGLMLHIMLMVKQGYRIVTLPKFEPVRFLELIKKYKVAMSFIVPPIAIMFAKSPIVDKFDLSSLRTLFCGAAPLGSEIEDLIKERFKGRLVIKQGYGATELSPCCFVTPNGLVKSGSSGTLL------------PNLLA--KIISSETGENL-GMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFLKTGDIGYVDEDGYFFIIDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKDFNNL 549          

HSP 2 Score: 72.0182 bits (175), Expect = 9.972e-12
Identity = 33/83 (39.76%), Postives = 49/83 (59.04%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE +++SHP V D  V   +   +G +PR FVV+K     T +E+L +AH ++A+  H RGGI F   +PK   GKL+
Sbjct:  457 VPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLL 539          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|126500|sp|P13129.1|LUCI_LUCCR (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 172.555 bits (436), Expect = 1.776e-44
Identity = 127/488 (26.02%), Postives = 230/488 (47.13%), Query Frame = 0

Query:  123 ICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPP-------PRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW------FFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE--------ENLTNLMESRLQDH--------ERIRGGLYFIQNIPRDENWKV----LRDVLQ 575
            I +C  N  E+++  +A    G  V P N       L   +  +K  ++       + V+ + +    +K ++++ ++    G   ++  I+ +  TPP         + +   E VA +  +S S+G   G+  TH++ +  F       +H  D + G+ ++ G        F H  G +T     I G  V  L+++ +  F++ + D K  +  L P    +L++S+ + KYDLS L  + S G  LS  +   +  RF  +  VR+ YGL E     +  P+      A+ KV        +P+       + K+I+  + +++ GP+ +GE+C+K      GY+N  E  K L D EG+ HTGD+GYYD +   + ++++++LI +  Y+V P  LES LL   +I DA V G+PD   GE        E+  N+ E  + D+        +R+RGG+ F+  +P+    K+    +R++L+
Sbjct:   80 IALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRN--TPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRF-------SHARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTSVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKV--------VPL------FKAKVIDLDTKKSL-GPNRRGEVCVKGPMLMKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKIDGRAIREILK 542          

HSP 2 Score: 63.5438 bits (153), Expect = 3.745e-9
Identity = 30/82 (36.59%), Postives = 49/82 (59.76%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKL 837
            + P  LE V++ HP++ D  V     P  G LP A VVL+SG   T +E++ +  +++++   LRGG+ F D++PK   GK+
Sbjct:  451 VPPAELESVLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKI 532          

BLAST of 4-coumarate-- ligase-like vs. SwissProt
Match: gi|266484|sp|Q01158.1|LUCI_LUCLA (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 168.703 bits (426), Expect = 3.750e-43
Identity = 122/488 (25.00%), Postives = 229/488 (46.93%), Query Frame = 0

Query:  123 ICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPP-------PRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW------FFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPD----------------KENGEENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKV----LRDVLQ 575
            I +C  N  E+++  LA    G  V P N       L   +  +K  ++       + V+ + +    +K ++++ ++    G   M++ I+ +  TP          + ++  E VA +  +S S+G   G+  TH++ +  F       +H  D + G+ ++ G        F H  G +T       G  +  L+++ +  F++ + D K  +  L P    +L++S+ + KYDLS L  + S G  LS  I   +  RF  +  VR+ YGL E     +  P+      A+ KV        +P+       + K+I+  + + + GP+ +GE+C+K      GY++  E  + + D EG+ HTGD+GYYD +   + ++++++LI +  Y+V P  LES LL   NI DA V G+PD                K   E+ + + + S++ + +R+RGG+ F+  +P+    K+    +R++L+
Sbjct:   80 IALCSENCEEFFIPVLAGLFIGVGVAPTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTAIKTIVILDSKVDYRGYQSMDNFIKKN--TPQGFKGSSFKTVEVNRKEQVALIMNSSGSTGLPKGVQLTHENAVTRF-------SHARDPIYGNQVSPGTAILTVVPFHHGFGMFTTLGYLTCGFRIVMLTKFDEETFLKTLQDYKCSSVILVPTLFAILNRSELLDKYDLSNLVEIASGGAPLSKEIGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKV--------VPL------FKAKVIDLDTKKTL-GPNRRGEVCVKGPMLMKGYVDNPEATREIIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPIAGELPGAVVVLEKGKSMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKIDGKAIREILK 542          

HSP 2 Score: 60.8474 bits (146), Expect = 2.920e-8
Identity = 29/82 (35.37%), Postives = 47/82 (57.32%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKL 837
            + P  LE V++ HP + D  V     P  G LP A VVL+ G   T +E++ +  +++++   LRGG+ F D++PK   GK+
Sbjct:  451 VPPAELESVLLQHPNIFDAGVAGVPDPIAGELPGAVVVLEKGKSMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKI 532          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1242782877|ref|XP_022331327.1| (4-coumarate--CoA ligase-like [Crassostrea virginica] >gi|1242782879|ref|XP_022331328.1| 4-coumarate--CoA ligase-like [Crassostrea virginica])

HSP 1 Score: 218.779 bits (556), Expect = 5.537e-58
Identity = 177/601 (29.45%), Postives = 293/601 (48.75%), Query Frame = 0

Query:   11 IMRNLTTPANMSRRGSSSSVGPPPLASSHQGRYKGLLSTPGVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAEN----TELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWF--FSPDGAANHYVDQMIGDSIACGNW-FFHMSGFYTVALAS-IYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENV-EGPDEQGEICIKSAQNFIGYLNEDNKRLFDS---EGFFHTGDLGYY-DSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE----------------ENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEPLST 582
            ++++ +T  N  RRGS     P    +  Q     +LS     +F   K E    ++ + +F+L +  EK  +KV L D    R  TY ++   + K+AS LT+LG+ K DVIC+   N  E+ ++ LAA   G  V   N       L  Q+  +KAK +         V D  E+    T+L K++ V    G++ GC P   L++DDG   P  +N+   EDV  LP++S ++G   G+M TH +L+A    F P       + ++  +  + G   FFH+ G   V +   I G  + +L ++    F++ +   K    ++ P  +  L +   +  +D+SCLK++ S    L   +  E+++R   V  +R+ YGL E      T P    + I        P    I   +PN  T  +++N +++E+V EG  E GEICI+  Q   GYLN  N++  D     G+ HTGD+G+Y   +GV    ++++ LI +   +VAP  LE  LL    + D AV+G+PD++ GE                 ++T+ +E ++  ++R+RGG+ FI  IP+  + K+LR +L+   P S 
Sbjct:   17 LLKSFSTVRN-GRRGSLCVSHP---VNFRQFSSSAVLSNVYSSRFGDLKLE----QKPLGQFMLERF-EKYGDKVALVDFPTDRKYTYAQLRIFIVKVASALTRLGYKKGDVICIHSVNIPEFSILLLAAASAGIIVTTSNPAYTAGELSRQLEHSKAKAVFTIPQLVPVVRDAIESGDSLTDLGKNITVF---GESEGCRPFATLMQDDGAAFPENVNIDPLEDVCVLPYSSGTTGLPKGVMLTHDNLVANLQQFRP-------ILKVTEEDTSLGILPFFHIYGMCPVMMGVLIDGGKLVTLPKFEPEMFLKALEVQKVTQLHIVPPIVLFLGKHPMVSNFDISCLKAITSGAAPLGEGLTNEVMDRLKAV--IRQGYGLTE------TSPVTHLDVIP-------PTCGTIGCVIPN--TLCRVLNPETDEDVPEG--EVGEICIRGPQVMKGYLN--NEKATDEMIRNGWLHTGDIGHYVKERGVFMITDRLKELIKYKGNQVAPAELEDLLLQHPAVQDVAVIGLPDEDGGEVPMAYIVKKANQEVSSHDITSFVEGKVAHYKRLRGGVEFIDQIPKSPSGKILRRILKESLPKSV 577          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|390364692|ref|XP_786981.3| (PREDICTED: 4-coumarate--CoA ligase 1 isoform X1 [Strongylocentrotus purpuratus])

HSP 1 Score: 217.238 bits (552), Expect = 7.842e-58
Identity = 152/526 (28.90%), Postives = 256/526 (48.67%), Query Frame = 0

Query:   71 EFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE---------------ENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYE 578
            +F+  K  E+  +   + D   GR+ T+ +V +   +IAS L++ G  K DVI +   N  EY L+     + GG V  VN     D L  Q+    A++IV      E  L+  +    ++ V V G   +A GC P   L+ DDG   P  + +S  ED   LPF+S ++G   G++ T+K++++     +   +   D   GD++     +FH  G   + L  +  G    ++S +    F++ I D K     L P  +  L++   + K+DLS +  +IS    L   +   L  R   +K +++ YGL E G V    P       +  K+L            PN  T+ K+++  S E + G  + GE+  +  Q   GYLN  E   R  D++GF HTGD+G+YD  G+ Y +++++ LI +  Y+VAP  LE+ LL   +I+DAAV+G+P++E GE               + +   +      ++++RGG+ F+++IP+  + K+LR VL+  E
Sbjct:   21 DFIYEKF-EQFGDSTAVADAASGRSYTFAQVRAYSRRIASALSRQGIKKGDVIGIVSPNLPEYVLMLCGVVEMGGIVSGVNPLYTEDELLHQMETVDARLIVTVPPFAEKCLNTMKRFPRVQDVYVFG---EAEGCKPFKSLLADDGSACP-DVKVSL-EDTFALPFSSGTTGLPKGVILTNKTIVSNLRQLESHPD-LTDTRPGDTVLALLPYFHCYGLVVIMLHGLRKGARQVTMSRFEPEVFLKTIQDYKVNHLYLVPPIMLFLAKHPVVDKFDLSSVSLIISGAAPLGGELTASLKTRL-GIKVIKQGYGLTESGPVLTLSPSSTDVPSSVGKLL------------PN--TEAKVVDTVSGE-LLGEGQDGELLFRGPQIMPGYLNNPEATARTLDADGFLHTGDIGHYDQDGLFYIVDRLKELIKYKGYQVAPAELETLLLTHPSIMDAAVIGVPNEEAGELPKAFIVPKNQELTADQVAEFVADNAAPYKKLRGGVEFVKSIPKSASGKILRRVLREKE 523          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1270089053|ref|XP_022808061.1| (4-coumarate--CoA ligase 1-like [Stylophora pistillata])

HSP 1 Score: 216.468 bits (550), Expect = 1.544e-57
Identity = 147/531 (27.68%), Postives = 261/531 (49.15%), Query Frame = 0

Query:   71 EFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNWFFHMSG-FYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE--------------ENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEPLSTEE 584
            EF+  +  E   ++  + D   G + T+ ++ +  +K AS L K GF K DV+ +   N  EY ++       GG V  VN     + LE Q+ + +A  +V      +      +    ++ + V+G EG +  C  ++ L++DDG + P    +  D DV  +P++S ++G   G+M TH S++A     + A    +DQ     +     F+H+ G    + +A   G  +  L  +    F++ + D K   A + P  I  L++   + K+DL+ ++ V S    +   +   L+ER P +K VR+ +G+ E  L+T   P  ++ S +              V LPN+  + KI++  +   V GP + GEIC++      GYLN  E   R+ D +G+ HTGD+G+YD  G  + +++++ LI +   +VAP  LE+ L+    I DAAV+G+PD++ GE              E + + +  R+  H+++ GG+ FI+ IP+  + K+LR  L+  E   T++
Sbjct:   20 EFVYERFDEFG-DRTAIIDGASGHSYTFTQLKTLTEKFASSLIKRGFQKGDVLAIYLPNVPEYPIIFYGVAYTGGKVTTVNPLYTAEELERQLQDTQAIYLVTSPPFLDKAQKACKVLGKIRKIFVVGGEG-SDDCEAVSSLLDDDGSSFPTECMVDKD-DVVTMPYSSGTTGLPKGVMITHHSMIANTCIIEAAKVFVIDQ--SSVVMTILPFYHIYGQLVMMGMALHQGGKLVVLQRFEPEPFLKTMQDQKVTNAPIVPPIILFLAKHPMVDKFDLTSVEEVTSGAAPMGKGLEEALIERMPKLKCVRQGFGMTEV-LITHVQPGGERKSGSVG------------VLLPNL--ECKIVDVTTG-AVLGPSQDGEICLRGPTIMKGYLNNPEATARMVDKDGWAHTGDIGHYDEDGHFFIVDRLKELIKYNALQVAPAELEALLISHQKIDDAAVIGVPDEKAGELPKAFVVPKGDITPEEIADFIAQRVAPHKKLSGGVEFIEKIPKSASGKILRKDLRKRESERTDK 529          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|675871274|ref|XP_009021197.1| (hypothetical protein HELRODRAFT_185750 [Helobdella robusta] >gi|555697327|gb|ESO00560.1| hypothetical protein HELRODRAFT_185750 [Helobdella robusta])

HSP 1 Score: 217.624 bits (553), Expect = 2.065e-57
Identity = 156/557 (28.01%), Postives = 261/557 (46.86%), Query Frame = 0

Query:   42 RYKGLLSTPGVVKFSAWKRETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFS---PDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE----------------ENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            R +   +T   + F +   +  IP   I EF+L     K   K  L D   GRT  + +V  +V ++ASG ++LGF K DV+ +   N +E+ LV  A    GG V   N       + +Q  +A    I C         +++E+   +K VI   T G+  G IP+  L+EDDG   P  +++ +    A LP++S ++G   G+M TH + +A       P+   N  V++ +G        F+H+ G     + S+  G +   +  +    F++ I   K    ++ P  +  L++   + ++D+S LK ++ +   L +    E  E++P    + + YG+ E      T P    N I T K     +   +P       T +K+++  +NE   GP E+GE+CIK  Q   GY    E    + DSEG+ HTGD+   D  G ++ +++++ LI +   +V P  LE+ L     + DAAVVG+PD E GE                E +   ++ R+  ++R+RGG+ F+  IPR    K+LR  L+N
Sbjct:   59 RARLFHTTSSNLVFKSRHPDVQIPNMSIPEFVL---DSKFTTKPALIDGVTGRTLNFNQVKDSVWRLASGFSRLGFKKGDVLMIISENSIEFMLVFHAIMWIGGTVTTCNPHYLVPDISKQAVDADVSYIFCSHLYSGKAHEVSEHMHTIKKVI---TVGQVDGLIPLTILLEDDGSEFPKNVDIDYKTHTALLPYSSGTTGMPKGVMLTHYNEVANAVQSDIPNFLLNRDVEKHLGLL-----PFYHIFGIIVHCICSMKDGQTTVIIPAFDPKIFLDCIQRYKIGMTHIVPPIVMFLNKHPMVKRFDMSSLKRLVCAAAPLGAESVHEFHEKYPNC-VLGQGYGMTE------TSPVVTMNQIPTCKTKPASSGTAVP------NTFLKVVDIYNNE--LGPGEKGELCIKGPQLMKGYHKNPEATANMIDSEGWLHTGDICTIDEDGHLFVVDRVKELIKYKGLQVPPAELEALLCSHKAVGDAAVVGVPDHEAGELPKAFVALKPGAKVTAEEIHKFVDERVVQYKRLRGGVVFLPQIPRSPAGKILRRELKN 589          

HSP 2 Score: 65.0846 bits (157), Expect = 5.122e-7
Identity = 34/83 (40.96%), Postives = 47/83 (56.63%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE ++ SH AV D +V        G LP+AFV LK G + TAEEI  F   R+     LRGG+VF  ++P+   GK++
Sbjct:  501 VPPAELEALLCSHKAVGDAAVVGVPDHEAGELPKAFVALKPGAKVTAEEIHKFVDERVVQYKRLRGGVVFLPQIPRSPAGKIL 583          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1176095348|ref|XP_020618234.1| (4-coumarate--CoA ligase 4-like [Orbicella faveolata])

HSP 1 Score: 224.557 bits (571), Expect = 2.584e-57
Identity = 153/521 (29.37%), Postives = 259/521 (49.71%), Query Frame = 0

Query:   79 EKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW-FFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHV----IPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQ 575
            E + +K+ + D   GR+ T+ ++A    K AS LTK GF K DV  +   N  E+ +V       GG V  +N     + +  Q+ +A    I+      + V +   N  +    +V    G+A GC  ++ L++DDG+  P  + ++  EDVA LP++S ++G   G+M TH +L+A        +  +  +   D+   G   FFH  G       ++Y G  V  + ++   +F+++I ++K   A L P  +  LS+S  + +YDLS LK V S    L   +   L ER P++++ R+ YG+ E   V                   TP D++    + V LPN+  + K+++ ++ + +  P+++GEIC+K      GYLN  E   +  +S+G+ HTGD+GYYD     + +++++ LI +  ++VAP  LE+ L+    I DAAV+G+PD   G                EE +   +E R+  H+ +RGG+ FI  IPR  + K+LR  L+
Sbjct:   27 EANGSKMAIVDGPSGRSYTFEQLAVLTKKFASALTKKGFKKGDVFAILLPNIPEFPIVYYGVIFIGGVVTTMNPLYTVEEITHQLQDAGVTSIITIPLFADKVKESTSNVGINTVYVV----GEAEGCESLSALLQDDGEDFPKNVEINPKEDVACLPYSSGTTGVPKGVMLTHYNLIACALIVTHESFMWNKE--EDAAVLGLLPFFHSFGQVISMSCALYKGAKVVCVPKFEPESFLKIIQEHKVSHAALVPSTVLFLSKSPLVEQYDLSSLKDVTSGAAPLGEELSKSLKERLPSIEWFRQGYGMTELSPVCHV----------------TPFDNIKYGSVGVLLPNL--ECKVVDIENGDEL-APNKEGEICVKGPTVMKGYLNNPEATAKTIESDGWLHTGDIGYYDEDEHFFIVDRLKELIKYKGFQVAPAELEALLVSHTAIEDAAVIGVPDARAGELPKAFVVKKANEEITEEEIAKYIEERVAPHKNLRGGVEFIDKIPRSLSGKILRRELK 522          

HSP 2 Score: 186.422 bits (472), Expect = 7.657e-45
Identity = 140/534 (26.22%), Postives = 250/534 (46.82%), Query Frame = 0

Query:   71 EFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAK-VIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAW--------FFSPDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            EF+     E + ++  ++D    R+ +Y +V     +IAS L K G  K DV  M   N  E+ +V     + GG +   N     + L +Q+ +A  K ++      +     M E      +V VIG      GC  ++ L+ DDG + P  + ++  ED+A LPF+S ++G   G++ TH +L++         F S D              +     FFH  G   +    ++ G ++ ++  +    F+  + + +     + P  + +L++  ++  +DLS L  V S    L   +   L +R P +  +R+ YG+ E   V    P +  N   +  VL           LPN+  + K+++ ++ EN+ G  + GEIC+K      GYLN  E   +  DS+G+ HTGD+G+YD     Y +++++ LI +  Y+V P  LE+ L+   +I DAAV+GIP+ E G                E+++   ++  +  ++++RGG+ FI  IP+  + K+LR  L++
Sbjct:  562 EFVFQHFDEYE-DREAMRDSITDRSYSYRQVKDLSKRIASALNKRGLKKGDVFAMLLPNLPEFPIVYFGVLQAGGVITAANPLFTTEELAKQLKQADTKWILTVPQLVHRAKEAMQELGR--SNVFVIG---DVDGCESLSGLLADDGSSFP-DVAINPKEDIAVLPFSSGTTGHPKGVILTHHNLISCGSIMRSKGFLSFDETT----------VLLAFLPFFHSYGMVAIMSMGLHCGSTIITMPRFEREKFLHALQNYEVTHLPVVPPVVLMLAKHPSVDNFDLSYLTEVTSGAAPLGKELSQALKDRLPLLSSIRQGYGMTELSPVCHMTPADN-NKHGSLGVL-----------LPNL--RCKVVDVETGENL-GHGQDGEICVKGPTVMKGYLNNPEATAQTIDSDGWLHTGDIGHYDEDEHFYIVDRVKELIKYNGYQVPPAELEALLISHPDIEDAAVIGIPNLEVGELPMAFVVRKANATLTEKDVEIFVDENVAPYKKLRGGVEFIDEIPKSLSGKILRRELKD 1063          

HSP 3 Score: 66.6254 bits (161), Expect = 2.287e-7
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLM 838
            + P  LE +++SH A++D +V        G LP+AFVV K+  E T EEI  +   R+A   +LRGG+ F DK+P+  +GK++
Sbjct:  435 VAPAELEALLVSHTAIEDAAVIGVPDARAGELPKAFVVKKANEEITEEEIAKYIEERVAPHKNLRGGVEFIDKIPRSLSGKIL 517          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|935661994|ref|WP_054493961.1| (4-coumarate--CoA ligase family protein [Ardenticatena maritima] >gi|913353104|dbj|GAP64242.1| hypothetical protein ARMA_2665 [Ardenticatena maritima] >gi|934115998|gb|KPL88640.1| AMP-dependent synthetase [Ardenticatena maritima])

HSP 1 Score: 215.698 bits (548), Expect = 2.623e-57
Identity = 159/544 (29.23%), Postives = 267/544 (49.08%), Query Frame = 0

Query:   61 ETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDS-IACGNW-FFHMSGFYTVALASIY-GVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEK-KNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDNK--RLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE--------------ENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYEPLSTEE 584
            +  IP   +  F+L K +E    K  L D   GRT TYG++A  +  +AS L K GFGK DV  +   N  EY +   A    GG V   N       L  Q+ +A AK ++         L+ A+       +  I   G+A G  P   L++ DG  P   + ++  EDV  LP++S ++G   G+M TH +L++     +G       ++I D+ +  G   F+H+ G   +  AS+Y G +V ++  +    F+ELI   K    +L P  +  L++   + KYDLS ++ +IS    L   +   + ER   +  V++ YG+ E   VT   P +  K  +A+   L           +PN  T+ +I++  + +++ GP+E+GE+ ++  Q   GYLN  +   +  DSEG+ HTGD+ Y D  G  Y +++++ LI +  Y+VAP  LE+ LL    + DAAV+ +PD+E GE              E +   +   +  ++++R  + F+++IP+  + K+LR VL   E +  E+
Sbjct:    9 DVQIPEMPLTPFVLRKAQELA-TKPALIDGPTGRTLTYGQLAGAIRLVASSLHKRGFGKGDVFAIYSPNLPEYAIAFHAVASLGGIVTTANPLYTAAELAHQLKDANAKYLLTIPL----FLENAKEAAAEAGIEEIFVFGEAEGATPFASLLQGDGVVP--EVEINPREDVVALPYSSGTTGLPKGVMLTHYNLVSNICQTEGI------EVIRDTDVLVGVLPFYHIYGMMVILNASLYHGATVVTMPRFDLEMFLELIQKYKISRMHLVPPILLALAKHPMVDKYDLSSVRMIISGAAPLGEELATAVRERLGCL--VKQGYGMTETSPVTHVNPDDPDKIKVASVGPL-----------VPN--TEARIVDPATGQDL-GPNERGELWVRGPQVMKGYLNRPDATTQTIDSEGWLHTGDVAYVDEDGYFYVVDRVKELIKYKGYQVAPAELEAVLLSHPAVADAAVIPVPDEEAGEIPKAFIVKKADVTAEEIMAYVAKHVAPYKKVR-EVEFVESIPKSPSGKILRRVLVEQERMKREQ 522          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|919073386|ref|XP_013417930.1| (4-coumarate--CoA ligase 1 [Lingula anatina])

HSP 1 Score: 215.698 bits (548), Expect = 3.378e-57
Identity = 155/541 (28.65%), Postives = 261/541 (48.24%), Query Frame = 0

Query:   61 ETDIPRQYIHEFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSL------MAWFFSPDGAANHYVDQMIGDSIACGNWFFHMSGFYTVALASI-YGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            +  IP   + +F+L KI + +   V L D   G   T+G V  +V +I S LT+LGF K DV C+   N  E+ L+ L     GG V   N +   D L +Q+     K +V    + +  L  A   ++ K V V G    A GC P +DL+ DDG   P  + +S  +DVA LPF+S ++G    +M TH ++      M  F     A    VD  +G        FFH+ G   + + ++ + + V  + ++    F+  I  +K +   + P    LL++   + +YDLS L++++S+   L   I  ++  R  T   V + YGL E        P ++K   + +  +   N            T+ K+++ Q+ + + GP++ GE+  + +  F GYLN  E      DS+ +FH+GD+G+YD    IY I++I+  I +  ++VAP  LE  L+  + + D AVVG PD++ G                E  + + + +++   +R+RGG+  +Q IP+  + K+LR VL+ 
Sbjct:    9 DVTIPDVDLAKFVLDKIDQYE-ESVALVDSQSGAKYTFGDVRDHVRRIGSALTRLGFKKGDVFCVYSPNVPEFALILLGVLAVGGTVTTANPQYTKDELSKQMKITPVKYMVTVPESSQKALQAAAENDI-KSVFVFG---HAPGCKPFHDLLRDDGHAFPHDVIIS-PQDVALLPFSSGTTGLPKAVMLTHHNIVGNLMQMREFLY---AGRPGVDGFLG-----FLPFFHLFGLAIILIGALKFRLKVVCMKKFDFEEFLACIETHKIRYLFVVPPIAILLAKHPLVDRYDLSSLETIMSAAAPLGKDIEQKVEARLKTCS-VTQGYGLTETSPACSLPPLDRKARRSGSSGILVAN------------TECKVVDPQTGKEL-GPNQDGELWFRGSHIFKGYLNNPEATAAAIDSDRWFHSGDIGHYDDDQYIYVIDRIKEFIKYKGFQVAPAELEGLLVTHSAVADTAVVGRPDEDAGELPMAFIVLKPGHTATETEIMDFLAAKVAPFKRLRGGVEIVQEIPKTASGKILRRVLRE 521          

HSP 2 Score: 70.4774 bits (171), Expect = 9.467e-9
Identity = 39/96 (40.62%), Postives = 56/96 (58.33%), Query Frame = 0

Query:  756 INPTVLEGVVMSHPAVDDCSVQAYTVPGLGGLPRAFVVLKSGYEATAEEILHFAHARLADTDHLRGGIVFADKLPKDGNGKLMCNV--EKLSQEAH 849
            + P  LEG++++H AV D +V        G LP AF+VLK G+ AT  EI+ F  A++A    LRGG+    ++PK  +GK++  V  EKL Q A 
Sbjct:  433 VAPAELEGLLVTHSAVADTAVVGRPDEDAGELPMAFIVLKPGHTATETEIMDFLAAKVAPFKRLRGGVEIVQEIPKTASGKILRRVLREKLRQRAQ 528          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|1109241711|ref|XP_019195543.1| (PREDICTED: 4-coumarate--CoA ligase-like 1 [Ipomoea nil])

HSP 1 Score: 215.312 bits (547), Expect = 8.757e-57
Identity = 170/563 (30.20%), Postives = 281/563 (49.91%), Query Frame = 0

Query:   71 EFLLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPPPRLNLSWDE-----DVAFLPFTSASSGCSAGIMHTHKSLMAWFFS------PDGAANHYVDQMIGDSIACGNW-FFHMSGFYTVALASIYGV-SVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLK--SVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTY--PKE-----KKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLNEDNKRLF--DSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENG----------------EENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVL-----QNYEPLSTEEISKQ 588
            +F+L  + E   +KV + D T G+  TYG+VA +V + A  L  LG  K  ++ +   N  EY ++AL     GG +   N       LE+QV  A AK+IV D   Y  V D+          +++  E +  G I   +L+E   +        S DE     D+  LPF+S ++G S G+M TH++L+A   S      PD         MIG  I  G   FFH+ G   +  A++     V ++  Y   AF++ ++ ++   A + P  I  L ++  + ++DLS LK  S++++   L+  I  E  ++FP ++ V+EAYG+ E   +TLT+  P++     KKNS+                 LPN+  +VK I+  S  ++   +  GE+C++S     GY N + +     D +G+ HTGD+GY D  G I+ +++I+ LI F  ++VAP  LE+ LL   ++ DAAVVG+PD+E G                EE++ N + S +  ++R+R  L+F+  IP+  + K++R V+     QN    S+ ++ K+
Sbjct:   35 DFVLHNV-ELYADKVAIVDATTGKEYTYGQVARDVRRFAKALRSLGLRKGRLVVVVLPNVAEYPIIALGIMAAGGVLSGANPTSHASELEKQVQVADAKLIVVDAPTYYKVKDLGLP-------VIVVDEERVQGTINWGELLEAADRASN-DFAFSEDEVVKQNDLCALPFSSGTTGMSKGVMLTHRNLVANLCSSLFSVGPD---------MIGQLIILGLIPFFHIYGLVGICCATMRNKGKVVAMRRYELRAFLDALITHEVNFAPIVPPIILGLVKNPIVDEFDLSKLKLKSIMTAAAPLAPEILNEFQKKFPGIQ-VQEAYGMTEHSCITLTHGDPRKGHGIAKKNSVGFI--------------LPNL--EVKFIDPDSGLSLPR-NTPGELCVRSQCVMKGYYNNEYETALTIDKDGWLHTGDIGYIDEDGDIFIVDRIKELIKFKGFQVAPAELEATLLSHPSVEDAAVVGLPDEEAGEVPAACVVLSAGAKDSEEDIMNYVASSVAHYKRVR-VLHFVDAIPKSHSGKIMRRVIKDKMVQNLTTASSHDVRKK 560          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|156400138|ref|XP_001638857.1| (predicted protein [Nematostella vectensis] >gi|156225981|gb|EDO46794.1| predicted protein [Nematostella vectensis])

HSP 1 Score: 214.542 bits (545), Expect = 9.086e-57
Identity = 155/535 (28.97%), Postives = 252/535 (47.10%), Query Frame = 0

Query:   69 IHEF--LLAKIKEKDPNKVWLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLI-EDDGKTPPPRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLM--AWFFSPDGAANHYVDQMIGDSIACGNW-FFHMSGF-YTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYP--KEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE--------------ENLTNLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQNYE 578
            IH F   + K  E    +  + D T GR+ TY K+   + K  S L + G   +D++ +   N  EY +V       GG V  +N     D +  Q+ ++ AK +V      +     A+   + +    I T G   GC  + DL+ EDDG + P  ++++W EDV  LP++S ++G   G+M TH +L+  A  FS D   +        D I  G    FH  G    + +  I G SV  ++++    F+E I   K     + P  +  L++   + KY+L  +K V S    L +     L+ R P +  +R+ YGL E      T P  K K  S+               V LP +  + K+I+ ++   +  P++ GEIC+       GY N  E   +  D EG+ HTGD+G+YD++G  Y +++I+ LI +  ++V P  LE+ LL    I DAAV+G+PD E GE                +   +  R+  H+R+RGG+  +Q++P++ + K+LR  L+  E
Sbjct:   17 IHSFPRYVLKHMESYGEETAMVDSTSGRSYTYNKLTEMIKKCGSALIRQGLQTKDMVAVLLPNMPEYPIVFYGVTSVGGIVTTINPAYTTDEIIYQLKDSGAKYLVTVPQLAQKAKQAADQAGVKR----IYTFGYVDGCKSLFDLLHEDDGGSYPVSVSVNWKEDVVCLPYSSGTTGLPKGVMLTHYNLIHHAAMFSNDDVMSS------EDLITLGLLPMFHSYGLSILMGVCLIKGASVICMTQFEPTHFLEAIQKFKITMLPVVPPIVLFLAKHPLVEKYNLMSIKQVTSGAAPLGAEQINALMTRMPWITILRQGYGLTETSPAVTTSPLGKCKPGSVG--------------VLLPGL--KAKVIDLKTGALLP-PNQDGEICVAGPTIMKGYYNNPEATAKTIDCEGWLHTGDVGHYDNEGHFYVVDRIKELIKYKGFQVPPAELEALLLSHPKITDAAVIGVPDDEAGELPKALVVTSGAITASEVQRFVAERVASHKRLRGGVEIVQSVPKNASGKILRRQLRERE 524          

BLAST of 4-coumarate-- ligase-like vs. nr
Match: gi|510861682|gb|EPB77084.1| (AMP-binding enzyme [Ancylostoma ceylanicum] >gi|597836286|gb|EYB85933.1| hypothetical protein Y032_0288g1483 [Ancylostoma ceylanicum])

HSP 1 Score: 213.772 bits (543), Expect = 2.330e-56
Identity = 149/544 (27.39%), Postives = 262/544 (48.16%), Query Frame = 0

Query:   69 IHEFLLAKIK---EKDPNKV-WLKDVTLGRTETYGKVASNVDKIASGLTKLGFGKRDVICMCCSNYVEYWLVALAAWKCGGCVMPVNCEIEFDLLEEQVTEAKAKVIVCDDFNYEDVLDMAENTELLKHVIVIGTEGKAGGCIPMNDLIEDDGKTPP---PRLNLSWDEDVAFLPFTSASSGCSAGIMHTHKSLMAWFFSPDGAANHYVDQMIGDSIACGNW------------FFHMSGFYTVALASIYGVSVYSLSEYSDNAFVELIVDNKTQTANLYPWQIRLLSQSKAIIKYDLSCLKSVISSGGVLSSTIRMELLERFPTVKYVREAYGLNECGLVTLTYPKEKKNSIATAKVLETPNDHVIPVGLPNMYTQVKIINRQSNENVEGPDEQGEICIKSAQNFIGYLN--EDNKRLFDSEGFFHTGDLGYYDSQGVIYYIEKIENLIHFWMYEVAPTVLESRLLGSNNIVDAAVVGIPDKENGE----------ENLT-----NLMESRLQDHERIRGGLYFIQNIPRDENWKVLRDVLQN 576
            +HE +LA +    +++P+++ ++       + T+ +V      +A+ L+K G G  DV C    N +EY +  L A  CGG +  V+       LE Q  ++  KV++ D+ +   VL   +    +K +I I + G      P     +D  KTP    P+ N S D D+A LP++S ++G   G++ TH++        +   ++++   +GDS    +W            F+H+ GF  +    I G +    +++    F+  I   K +   L P  + LL++      YDLS L+ V++        +  E L ++  V+Y+ +AYG+ ECG+ +   P + +++  T   L +             + Q  I     NE   G  E+GEIC++S     GYLN  E    + DSEG+ HTGD+G+ D +G  + +++++ LI     +VAP  LE  LL    I DAAV+G+PD++ GE          +NLT       +E ++  ++ + GG+ F++ IP+  + K+LR  L++
Sbjct:   17 LHEKVLAAVLRNIDRNPDRIAFISAEDPSASITFKEVYGQAHSVANFLSKRGHGHLDVCCQVMQNCIEYAVFYLGALLCGGAISGVSAMFTTYELERQFVDSHCKVVITDEAHLNKVLLATKRCPEVKTIICIRSPGSTHALPPAVVSWDDVIKTPVSALPKYNYSPD-DMALLPYSSGTTGSPKGVVLTHRNFTTMLDIVNSHFDNHIVSKLGDS----DWDYHNENLLLMLPFYHIYGFGLMNQTLITGTTGIVFAKFDPTVFLNAIQTYKPKMLMLVPPILLLLTKHPMTSNYDLSSLQFVLTGAAPAGKDLCDEFLNKYKHVRYLAQAYGMTECGMASHLPPLDSRSNFLTVGTLIS------------TFEQKIISTSTGNEVPIG--ERGEICVRSPTIMKGYLNRPEATAEMIDSEGWLHTGDIGFVDEKGQTFIVDRLKELIKVKGLQVAPAELEDILLSHKEIHDAAVIGVPDEKTGEKPRAYVVSASKNLTEADVKRFVEEKVSTYKFLTGGVVFVKEIPKSPSGKILRRFLRD 541