BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000011454 (supercontig:LSalAtl2s:LSalAtl2s790:183804:208736:-1 gene:EMLSAG00000011454 transcript:EMLSAT00000011454 description:"maker-LSalAtl2s790-augustus-gene-2.32")

HSP 1 Score: 607.831 bits (1566), Expect = 0.000e+0
Identity = 325/725 (44.83%), Postives = 430/725 (59.31%), Query Frame = 0

Query:  202 NENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIKAGADTLVNSIGPGPKSTPSHWAGGTPVMGHCVTLKSCFPLFNYQANTPSWMMGEKDMCQTTEPTTRQSMA-GVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVHPGIPGVVQPPPPSYPVHAPIMTTPTTTTTT-TTTTVWTTTTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRY-TGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            +++CG+R   +NL +IVNG +A   EFPFMAALF   R F GGS++D  HILTAAHCV HM++ DVR ++V++  + +++     EQ+FRV+ V+RHK F  +TL+NDVA+LTL  +  F+ +VQPICL  D    YEG  V VAGWG++++ G QS+IL+KV V VWSN  C  SYG++APGGI +HM+CAA   +DSCSGDSGGPLF C   C QIGIVSWGIG      P  Y                 D  +G  + +      P ++ +  +G       C++ KSC+P    +++ P W+ G+ ++C+   P   Q     +CC          YP               IYP   I + PG  G                 P + +          P++T+ TT+     T +  TT+T+ TT          P          CG   H+PI Y ENG   E + RI+ EH L++VNGWPA K+EWPWIAAL   GRQFCGGSLI  +HILTAAHCVAQM+  DV  L VRLGEHNI+   ++      V +++RHKGFSS TL+NDVAL+ L +  P S SD I+P+C+ +    Y      TVVGWGSLRE+GPQP +LQE+T+ +W N +C + Y G SPAGI DHM+CAG++GKDSCSGDSGGP +   GD+W Q+GIVSWGIGCGK+ FPGVYTR   +  WI +   KF
Sbjct:  238 SDHCGQRFFSNNL-KIVNGEDASVDEFPFMAALFNKXRHFXGGSIIDSKHILTAAHCVAHMTKIDVRYLRVHVRQYRIKSSNIGTEQEFRVRRVIRHKSFSANTLYNDVAILTLRGSIDFSKSVQPICLASDNTETYEGDIVTVAGWGTLSDGGRQSSILQKVSVGVWSNYDCGASYGSRAPGGIKSHMLCAASKGKDSCSGDSGGPLFYCDTYCTQIGIVSWGIGSK----PDSYDD---------------DHPSGQSSNIYQHSKFPCTSSNGRSG------RCISFKSCYPFHRVRSDVPEWVFGDANVCKNRNPREAQDQRREICCENVYQSILGFYP---------------IYPVHVI-YVPGVDG-----------------PILTETISGXDXQFLPVITSTTTSXLRPITISTDTTSTSTTTIVKETTTTSRPDNLVINDQSVCGNSIHYPIVYDENGSVSEEINRIDMEHDLKIVNGWPASKHEWPWIAALFNRGRQFCGGSLISRSHILTAAHCVAQMTRSDVNNLEVRLGEHNIKDALDTQVEVRKVNKIIRHKGFSSQTLWNDVALLFLSD--PVSYSDIIKPICIDTSDDPYDKNXTATVVGWGSLRESGPQPXILQEVTLRLWKNEKCQETYNGASPAGITDHMICAGRQGKDSCSGDSGGPMVKAKGDKWVQIGIVSWGIGCGKSHFPGVYTRVGKMYEWITKVIEKF 901          

HSP 2 Score: 266.159 bits (679), Expect = 4.429e-76
Identity = 184/592 (31.08%), Postives = 279/592 (47.13%), Query Frame = 0

Query:   28 FIDGDAIVLEVTEDDLTSSGGQKLERSDNPFVSSESRRARQFPFSAPGVPFLQPVGGLPLQPSFVPARSNLPQFQQPPVQAPLGNACVTNEGQNGTCTTVKECYPYFRLFNFDADETSTFGTYDTCSIDTRAGKKIFGICCVEKNIKADLLAEIEALRTEEPINPDEFPLDAQVNEN------------------CGKRSVGSNLVRIVNGTEAKQHEFPFMA-----------------ALFKNSRQFCGG------------SLLDKTH------ILTAAH------CVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIKAGADTLVNSIGPGPKSTPSHWAGGTPVMGHCVTLKSCFPLFNYQANTPSWMMGEKDMCQTTEPTTRQSM-AGVCCSTRQ---FGQYPLYPQYPGILGVPGAQG 556
            F D D++V+E   DD+     + ++R D  F     R  RQ P     +PF        L  S   + +N+ Q             C+T+ G+ G CT++K CYPYF+  N     T   G YDTCS   +  +++FG+CC    + A+ + +  +    +P  P  FP+  Q + N                    +++       I+ G E ++ E                        F N+ +   G            +L +K        I+ + H      CV HM++ DVR ++V++  + +++     EQ+FRV+ V+RHK F  +TL+NDVA+LTL  +  F+ +VQPICL  D    YEG  V VAGWG++++ G QS+IL+KV V VWSN  C  SYG++APGGI +HM+CAA   +DSCSGDSGGPLF C   C QIGIVSWGIG      P  Y                 D  +G  + +      P ++ +  +      G C++ KSC+P    +++ P W+ G+ ++C+   P   Q     +CC        G YP+YP +  ++ VPG  G
Sbjct:   40 FGDEDSVVIE---DDID----RHVDREDLGF----DRNDRQLP----NIPF-------DLNSSKKNSGANIYQ------------PCLTSTGRLGRCTSLKVCYPYFKSPNIIGSNTWIKGLYDTCSYIGKRAQQVFGVCCTGIQMAAEEIKD-SSENNNKPTQP-PFPIKRQEDNNKPTQPSLPIKKQEDNIEVTHQKNSLEKDYDIMEGEEDQELEIRNTTNTKDLLGVKLSSDHCGQRFFSNNLKIVNGEDASVDEFPFMAALFNKXRHFXGGSIIDSKHILTAAHCVAHMTKIDVRYLRVHVRQYRIKSSNIGTEQEFRVRRVIRHKSFSANTLYNDVAILTLRGSIDFSKSVQPICLASDNTETYEGDIVTVAGWGTLSDGGRQSSILQKVSVGVWSNYDCGASYGSRAPGGIKSHMLCAASKGKDSCSGDSGGPLFYCDTYCTQIGIVSWGIGSK----PDSYDD---------------DHPSGQSSNIYQHSKFPCTSSNGRS------GRCISFKSCYPFHRVRSDVPEWVFGDANVCKNRNPREAQDQRREICCENVYQSILGFYPIYPVH--VIYVPGVDG 568          

HSP 3 Score: 243.817 bits (621), Expect = 3.347e-68
Identity = 119/264 (45.08%), Postives = 173/264 (65.53%), Query Frame = 0

Query:  189 PINPDEFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCT-DRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            PI  DE   +  V+E   +  +  +L +IVNG  A +HE+P++AALF   RQFCGGSL+ ++HILTAAHCV  M+++DV  ++V LG+HN++   +   +  +V  ++RHK F + TL NDVA+L LS+   ++  ++PIC++       +  +  V GWGS+ E G Q  IL++V + +W N+KC+ +Y   +P GIT HMICA    +DSCSGDSGGP+     D+  QIGIVSWGIGC +  FPGVYTRV ++  WI K++
Sbjct:  639 PIVYDE---NGSVSEEINRIDMEHDL-KIVNGWPASKHEWPWIAALFNRGRQFCGGSLISRSHILTAAHCVAQMTRSDVNNLEVRLGEHNIKDALDTQVEVRKVNKIIRHKGFSSQTLWNDVALLFLSDPVSYSDIIKPICIDTSDDPYDKNXTATVVGWGSLRESGPQPXILQEVTLRLWKNEKCQETYNGASPAGITDHMICAGRQGKDSCSGDSGGPMVKAKGDKWVQIGIVSWGIGCGKSHFPGVYTRVGKMYEWITKVI 898          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000012442 (supercontig:LSalAtl2s:LSalAtl2s914:161702:164343:-1 gene:EMLSAG00000012442 transcript:EMLSAT00000012442 description:"maker-LSalAtl2s914-augustus-gene-2.11")

HSP 1 Score: 210.305 bits (534), Expect = 4.525e-59
Identity = 101/247 (40.89%), Postives = 148/247 (59.92%), Query Frame = 0

Query:  679 EHPLRVVNGWPADKNEWPWIAALLR----SGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNG-DRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERAR 920
             +  R+V G PAD NEWPW+AA++R    S  QFCG +LI   H++TAAHCVA      ++   VRLGE+N   EGE+      +  +  H+ + +   YND+A++ L    P + S +I P+CLP     Y  R G V+GWG++   GP  ++LQE+ + +WDN +C + Y  ++   +   M+CAG +G+D+C GDSGGP    N   +WE  G+VSWG  C + D+PGVYTR T+   WIE+ +
Sbjct:  287 RYSTRIVGGRPADPNEWPWLAAMIRPSEVSSGQFCGATLISDRHVITAAHCVAPFKQDQIK---VRLGEYNFNAEGETLDRTYNLESIKMHELYDAVKYYNDIAIMKLDR--PVTFSKSIYPICLPPPIETYEKRRGYVIGWGTIYFGGPTSNILQEVNVAVWDNNKCKENYSALN-RNVTSKMLCAGDRGRDACQGDSGGPLNCLNAFSQWELCGVVSWGARCAEPDYPGVYTRVTEYLDWIEQNK 527          

HSP 2 Score: 188.348 bits (477), Expect = 2.217e-51
Identity = 96/241 (39.83%), Postives = 147/241 (61.00%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFK----NSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQ---IGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AA+ +    +S QFCG +L+   H++TAAHCV    Q+   Q+KV LG++N    GE  ++ + ++++  H+ +     +ND+A++ L     F+ ++ PICL    +  YE R   V GWG+I   G  S IL++V+V VW N+KC+ +Y +     +T+ M+CA    RD+C GDSGGPL +C +   Q    G+VSWG  CA P +PGVYTRVTE + WI++
Sbjct:  291 RIVGGRPADPNEWPWLAAMIRPSEVSSGQFCGATLISDRHVITAAHCVAPFKQD---QIKVRLGEYNFNAEGETLDRTYNLESIKMHELYDAVKYYNDIAIMKLDRPVTFSKSIYPICLPPP-IETYEKRRGYVIGWGTIYFGGPTSNILQEVNVAVWDNNKCKENY-SALNRNVTSKMLCAGDRGRDACQGDSGGPL-NCLNAFSQWELCGVVSWGARCAEPDYPGVYTRVTEYLDWIEQ 525          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000007886 (supercontig:LSalAtl2s:LSalAtl2s464:52646:53754:1 gene:EMLSAG00000007886 transcript:EMLSAT00000007886 description:"maker-LSalAtl2s464-augustus-gene-0.4")

HSP 1 Score: 165.236 bits (417), Expect = 4.752e-46
Identity = 93/235 (39.57%), Postives = 135/235 (57.45%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAAL-FKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            +IV GTEA+ H  PF  +   KN   FCGGS+LD+T ++TA HC +  S NDV   +V +G H+  +  E  EQ   +  +  H+ F +  ++ND+ +L +     F   V+P+ L +   F   G  V V+GWG++  +G+ S +LR V + +    +C  +Y     GG+   MICA+G  +DSC GDSGGPL         +GIVSWGIGCA P FPGVYT+V+  + WI +
Sbjct:   38 KIVGGTEAQPHSIPFQVSFQRKNGFHFCGGSILDETTVITAGHCCKGFSINDV---QVVVGAHDFNSP-EGTEQTQNIVKITYHENFASKGINNDICLLEVEHPFEFNDNVKPVTLPEKE-FTPTGEVV-VSGWGTLRANGNSSPVLRTVTLNMVPYLRCYINY----IGGLDESMICASGKGKDSCQGDSGGPL---VQENTLVGIVSWGIGCAHPWFPGVYTKVSMFIDWIHE 259          

HSP 2 Score: 150.984 bits (380), Expect = 3.723e-41
Identity = 90/240 (37.50%), Postives = 131/240 (54.58%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLR-SGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRR-EGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERAR 920
            ++V G  A  +  P+  +  R +G  FCGGS++D T ++TA HC    S  DVQ++   +G H+    EG   T    + ++  H+ F+S  + ND+ L+ +++  P   +D ++PV LP      TG +  V GWG+LR NG    VL+ +T+ +    RC   Y G    G+ + M+CA  KGKDSC GDSGGP +  N      VGIVSWGIGC    FPGVYT+ +    WI   +
Sbjct:   38 KIVGGTEAQPHSIPFQVSFQRKNGFHFCGGSILDETTVITAGHCCKGFSINDVQVV---VGAHDFNSPEGTEQT--QNIVKITYHENFASKGINNDICLLEVEH--PFEFNDNVKPVTLPEKEFTPTGEV-VVSGWGTLRANGNSSPVLRTVTLNMVPYLRCYINYIG----GLDESMICASGKGKDSCQGDSGGPLVQEN----TLVGIVSWGIGCAHPWFPGVYTKVSMFIDWIHENK 261          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000008660 (supercontig:LSalAtl2s:LSalAtl2s537:130430:177832:-1 gene:EMLSAG00000008660 transcript:EMLSAT00000008660 description:"maker-LSalAtl2s537-augustus-gene-2.26")

HSP 1 Score: 176.022 bits (445), Expect = 2.749e-45
Identity = 92/239 (38.49%), Postives = 138/239 (57.74%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSG--RQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYN-DVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG-KKGK-DSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            R+V G   ++NE+PW   +++ G  + FCGG+L+   H++TA HC      Q    + + LGEHNI+   ++     PV +V  HK FS   + + D+ L+TL+   P      I+P+CLP     + G + T+ GWG L E GPQPD+LQE+ + +  N  C + Y+ ++P      M+CAG KKG+ D+C GDSGGP      +R+   G  S+G GC + DFPGVY+R T ++ WI
Sbjct:  827 RIVGGKEVEENEYPWHIGIVKIGDNKPFCGGALVTDRHVVTAGHCF-----QTFHKVEILLGEHNIQNSNDT-ALRIPVKKVEVHKEFSVLAMNDYDITLLTLER--PIKFRREIQPICLPFKDEDFVGTMCTITGWGRLNETGPQPDILQEVNVPVITNDVCKKSYETLTP-----RMICAGYKKGELDACQGDSGGPLSCPQNNRYVLAGSTSYGFGCARPDFPGVYSRITLLQDWI 1052          

HSP 2 Score: 164.466 bits (415), Expect = 1.083e-41
Identity = 91/241 (37.76%), Postives = 136/241 (56.43%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQ--FCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYN-DVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG-KKGK-DSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            R+V G   ++NE+PW   +++ G+   FCGGSL+   H++TAAHC   +       + V LGEHNI+   ++     PV +V  HK F      + DV L+TL+   P      I+P+CLP     + G + T+ GWG L E  PQPD+LQE+ + +  N  C++ Y+ ++P      M+CAG +KG+ DSC GDS  P      +R+   GI S+G GC +  FPGVY+R T ++ WI+ 
Sbjct:  162 RIVGGKEVEENEYPWQIGIVKIGKNKPFCGGSLVSDRHVVTAAHCFKTLYK-----VEVLLGEHNIQNSNDT-ALRIPVKKVEVHKEFGVLAFNDYDVTLLTLEQ--PIKFRREIQPICLPFKDEDFVGTMCTITGWGRLSETEPQPDILQEVNVPVITNDVCNKAYETITP-----RMICAGYEKGEIDSCQGDS--PLSCPQNNRYVFTGITSFGHGCARPGFPGVYSRITLLQDWIKE 387          

HSP 3 Score: 156.377 bits (394), Expect = 4.046e-39
Identity = 95/255 (37.25%), Postives = 143/255 (56.08%), Query Frame = 0

Query:  205 CGKRSVGSNLVRIVNGTEAKQHEFPFMAALFK--NSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHN-DVAVLTLSEAAVFTPTVQPICL---EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTF--RDSCSGDSGGPLFDCTDRCRQI--GIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            CG ++V S   RIV G E +++E+P+   + K  +++ FCGG+L+   H++TA HC +        ++++ LG+HN++   + A  +  VK V  HK F    +++ D+ +LTL     F   +QPICL   ++D    + G    + GWG + E G Q  IL++V+V V +ND C++SY    P      MICA       D+C GDSGGPL  C    R +  G  S+G GCARP FPGVY+R+T L  WI +
Sbjct:  819 CGTKNVNS---RIVGGKEVEENEYPWHIGIVKIGDNKPFCGGALVTDRHVVTAGHCFQTF-----HKVEILLGEHNIQNSNDTA-LRIPVKKVEVHKEFSVLAMNDYDITLLTLERPIKFRREIQPICLPFKDED----FVGTMCTITGWGRLNETGPQPDILQEVNVPVITNDVCKKSYETLTP-----RMICAGYKKGELDACQGDSGGPL-SCPQNNRYVLAGSTSYGFGCARPDFPGVYSRITLLQDWINE 1054          

HSP 4 Score: 149.058 bits (375), Expect = 8.480e-37
Identity = 99/256 (38.67%), Postives = 145/256 (56.64%), Query Frame = 0

Query:  205 CGKRSVGSNLVRIVNGTEAKQHEFPFMAALFK--NSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHN-DVAVLTLSEAAVFTPTVQPICL---EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICA---AGTFRDSCSGDSGGPLFDCTDRCRQI--GIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            CG ++V S   RIV G E +++E+P+   + K   ++ FCGGSL+   H++TAAHC + + +     ++V LG+HN++   + A  +  VK V  HK F     ++ DV +LTL +   F   +QPICL   ++D    + G    + GWG ++E   Q  IL++V+V V +ND C ++Y       IT  MICA    G   DSC GDS  PL  C    R +  GI S+G GCARP FPGVY+R+T L  WI++
Sbjct:  154 CGTKNVNS---RIVGGKEVEENEYPWQIGIVKIGKNKPFCGGSLVSDRHVVTAAHCFKTLYK-----VEVLLGEHNIQNSNDTA-LRIPVKKVEVHKEFGVLAFNDYDVTLLTLEQPIKFRREIQPICLPFKDED----FVGTMCTITGWGRLSETEPQPDILQEVNVPVITNDVCNKAYET-----ITPRMICAGYEKGEI-DSCQGDS--PL-SCPQNNRYVFTGITSFGHGCARPGFPGVYSRITLLQDWIKE 387          

HSP 5 Score: 108.997 bits (271), Expect = 2.017e-24
Identity = 57/160 (35.62%), Postives = 88/160 (55.00%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQ--FCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYN-DVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDN 839
            R+V G   ++NE+PW   +++ G    FCGG+L+   H++TA HC      Q  + + + LGEHNIR   ++      V +V  HK F+     + D+ L+TL+   P      I+P+CLP     + G + T+ GWG L E GPQPD+LQE+ + +  N
Sbjct:  533 RIVGGKEVEENEYPWHIGIVKIGENLPFCGGALVTDRHVVTAGHCF-----QTFRKVEILLGEHNIRNSHDT-ALRIRVKKVEVHKEFNIIAKNDYDITLLTLER--PIKFRREIQPICLPFKDDDFVGTMCTITGWGRLNETGPQPDILQEVNVPVITN 684          

HSP 6 Score: 101.293 bits (251), Expect = 5.785e-22
Identity = 61/179 (34.08%), Postives = 100/179 (55.87%), Query Frame = 0

Query:  205 CGKRSVGSNLVRIVNGTEAKQHEFPFMAALFK--NSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHN-DVAVLTLSEAAVFTPTVQPICL--EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSY 378
            CG ++V S   RIV G E +++E+P+   + K   +  FCGG+L+   H++TA HC +       R++++ LG+HN+R   + A  + RVK V  HK F     ++ D+ +LTL     F   +QPICL  + D   ++ G    + GWG + E G Q  IL++V+V V +ND  + ++
Sbjct:  525 CGTKNVNS---RIVGGKEVEENEYPWHIGIVKIGENLPFCGGALVTDRHVVTAGHCFQTF-----RKVEILLGEHNIRNSHDTA-LRIRVKKVEVHKEFNIIAKNDYDITLLTLERPIKFRREIQPICLPFKDD---DFVGTMCTITGWGRLNETGPQPDILQEVNVPVITNDDFQSAF 691          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000002149 (supercontig:LSalAtl2s:LSalAtl2s139:22566:31405:1 gene:EMLSAG00000002149 transcript:EMLSAT00000002149 description:"maker-LSalAtl2s139-augustus-gene-0.10")

HSP 1 Score: 159.844 bits (403), Expect = 7.417e-42
Identity = 90/247 (36.44%), Postives = 140/247 (56.68%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSG----RQFCGGSLIDSTHILTAAHCV--AQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTV---VGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKK--GKDSCSGDSGGPFMIGNGDR--WEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            R+V G    + E+PW+AA+   G      +CGG+L+   H++TAAHC   A+      +  T+RLGE ++  + E+ + E  +  V+ H  F ++  Y+DVA+  LK   P   S+ I+P+CLP+GS +    LGT+   +GWG+    G + + L+ + + +W+N  CD  Y    P  I +  +CAG    G+D+C GDSGGP M+ +  R  W  +G+VS+G  C +A +PGVYTR T    WI
Sbjct:  253 RIVGGKATYEGEYPWMAAIYLHGGGRTEFWCGGALVSERHVITAAHCTKDAKKKQFKARQFTIRLGEWDLD-DKETYSREYEIVDVIAHPDFKANGFYSDVAI--LKTIEPVEFSEYIQPICLPTGSMKNDNFLGTLPVALGWGATHYRGKEVNKLRGVALPVWNNKDCDGAY--FQP--ITEVFLCAGYASGGRDACQGDSGGPLMLYSQKRKAWVLIGVVSFGNRCAEAGYPGVYTRLTHFLDWI 492          

HSP 2 Score: 142.895 bits (359), Expect = 3.218e-36
Identity = 86/245 (35.10%), Postives = 132/245 (53.88%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKN----SRQFCGGSLLDKTHILTAAHCVE--HMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFN--YEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICA--AGTFRDSCSGDSGGPLFDCTDRCRQ---IGIVSWGIGCARPVFPGVYTRVTELMPWI 447
            RIV G    + E+P+MAA++ +    +  +CGG+L+ + H++TAAHC +     Q   RQ  + LG+ +L  + E   +++ +  V+ H  FK +  ++DVA+L   E   F+  +QPICL    + N  + G      GWG+    G +   LR V + VW+N  C  +Y       IT   +CA  A   RD+C GDSGGPL   + + +    IG+VS+G  CA   +PGVYTR+T  + WI
Sbjct:  253 RIVGGKATYEGEYPWMAAIYLHGGGRTEFWCGGALVSERHVITAAHCTKDAKKKQFKARQFTIRLGEWDLDDK-ETYSREYEIVDVIAHPDFKANGFYSDVAILKTIEPVEFSEYIQPICLPTGSMKNDNFLGTLPVALGWGATHYRGKEVNKLRGVALPVWNNKDCDGAYFQP----ITEVFLCAGYASGGRDACQGDSGGPLMLYSQKRKAWVLIGVVSFGNRCAEAGYPGVYTRLTHFLDWI 492          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000004828 (supercontig:LSalAtl2s:LSalAtl2s254:397245:398159:-1 gene:EMLSAG00000004828 transcript:EMLSAT00000004828 description:"augustus_masked-LSalAtl2s254-processed-gene-4.1")

HSP 1 Score: 150.599 bits (379), Expect = 6.290e-41
Identity = 92/237 (38.82%), Postives = 133/237 (56.12%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAAL-FKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNL-RTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICL-EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G E + +  PF  +        FCG S++DK  I+TAAHC +    ++V+   V  G+H+L  T G+  EQ+  V  +  H++F +   + DV +L L  +  F   V+PI L E+D  F  +   V V+GWG+I+  G  S +L+ V V+V S++ C  +Y     G I   MICAA   +DSC GDSGGPL         +GIVSWG GCA P +PGVY +V++ + WI +
Sbjct:   41 RIVGGEEVEPNSIPFQISFQTTGGFHFCGASVMDKDTIITAAHCCDSFYPSEVQ---VVAGEHDLFSTSGD--EQKIAVSDITYHEKFASHGTNYDVCLLKLKSSLHFNEKVKPIALPEKDQEFIGD---VVVSGWGTISSSGPPSPVLKAVTVQVVSDEDCSDAY----YGSIDETMICAAAPGKDSCQGDSGGPL---AQDGTLVGIVSWGYGCAAPGYPGVYGKVSKFIDWIAE 262          

HSP 2 Score: 134.806 bits (338), Expect = 1.515e-35
Identity = 83/261 (31.80%), Postives = 132/261 (50.57%), Query Frame = 0

Query:  661 ISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPW-IAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERAR 920
            I Y E+   V++++   G    R+V G   + N  P+ I+     G  FCG S++D   I+TAAHC       +VQ++    GEH++      +  +  V+ +  H+ F+S     DV L+ LK+++    ++ ++P+ LP     + G +  V GWG++  +GP   VL+ +T+++  +  C   Y G     I + M+CA   GKDSC GDSGGP           VGIVSWG GC    +PGVY + +    WI   +
Sbjct:   22 IKYSESFMKVKSMRHRFGG---RIVGGEEVEPNSIPFQISFQTTGGFHFCGASVMDKDTIITAAHCCDSFYPSEVQVVA---GEHDLFST-SGDEQKIAVSDITYHEKFASHGTNYDVCLLKLKSSL--HFNEKVKPIALPEKDQEFIGDV-VVSGWGTISSSGPPSPVLKAVTVQVVSDEDCSDAYYG----SIDETMICAAAPGKDSCQGDSGGPL----AQDGTLVGIVSWGYGCAAPGYPGVYGKVSKFIDWIAEHQ 264          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000012246 (supercontig:LSalAtl2s:LSalAtl2s892:54138:171852:1 gene:EMLSAG00000012246 transcript:EMLSAT00000012246 description:"maker-LSalAtl2s892-augustus-gene-0.10")

HSP 1 Score: 154.836 bits (390), Expect = 1.275e-40
Identity = 86/235 (36.60%), Postives = 124/235 (52.77%), Query Frame = 0

Query:  690 ADKNEWPWIAALLRSGRQF--CGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFET-PVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGT--VVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQG-VSPA-GIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIE 917
            AD  EWPW AA+L   +    CG +L+D + +LTAAHCV            VRLGE+++    E    E   VT +V H GF++ TL +D+AL+ L+   P      I  VC+P        +     V GWG   E      VL+E+ + +W    C+   +    PA  +    +CAG +G+D+C GD GGP +     +W Q+GIVS+GIGCG+ + PGVYT+ ++   WIE
Sbjct:  189 ADMAEWPWHAAILEKPQDLYVCGSTLLDESWVLTAAHCVDDYLNTWYIFSIVRLGEYDVSTTAEPLRHEEFNVTDIVMHPGFNNSTLVHDIALLKLER--PAKRKQNIDVVCMPKSRDFNPSQFARCYVTGWGRRTETSEHSLVLKEIEVPLWSYTNCNGALKAQFGPAYSLPTXAICAGAEGRDACDGDGGGPLVCEKEGKWYQIGIVSFGIGCGRKNIPGVYTKVSEXDSWIE 421          

HSP 2 Score: 140.969 bits (354), Expect = 5.371e-36
Identity = 88/254 (34.65%), Postives = 132/254 (51.97%), Query Frame = 0

Query:  205 CGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQF--CGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGE-AAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFN-YEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQ-APG-GITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            CG++   S      N   A   E+P+ AA+ +  +    CG +LLD++ +LTAAHCV+           V LG++++ T  E    ++F V  +V H  F  STL +D+A+L L   A     +  +C+ +   FN  +     V GWG   E    S +L++++V +WS   C  +   Q  P   + T  ICA    RD+C GD GGPL  +   +  QIGIVS+GIGC R   PGVYT+V+E   WI+ ++
Sbjct:  176 CGRKGFDSG-----NSGGADMAEWPWHAAILEKPQDLYVCGSTLLDESWVLTAAHCVDDYLNTWYIFSIVRLGEYDVSTTAEPLRHEEFNVTDIVMHPGFNNSTLVHDIALLKLERPAKRKQNIDVVCMPKSRDFNPSQFARCYVTGWGRRTETSEHSLVLKEIEVPLWSYTNCNGALKAQFGPAYSLPTXAICAGAEGRDACDGDGGGPLVCEKEGKWYQIGIVSFGIGCGRKNIPGVYTKVSEXDSWIENVI 424          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000010925 (supercontig:LSalAtl2s:LSalAtl2s730:283497:284811:1 gene:EMLSAG00000010925 transcript:EMLSAT00000010925 description:"maker-LSalAtl2s730-augustus-gene-3.19")

HSP 1 Score: 143.665 bits (361), Expect = 6.248e-39
Identity = 91/235 (38.72%), Postives = 128/235 (54.47%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAAL-FKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNL-RTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICL-EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWI 447
            RIV G E + +  PF  +        FCG S++DK  I+TAAHC +     +V+   V  G+H+L  T G+  EQ   V  +  H+ F +   + DV +L L  +  F   V+PI L E+D  F  +   V V+GWG+I+  G  S +L+ V V+V S++ C  +Y     G I   MICAA   + SC GDSGGPL         +GIVSWG GCA P +PGVY +V++ + WI
Sbjct:   13 RIVGGEEVEPNSIPFQVSFQTTGGFHFCGASVMDKDTIITAAHCCDSFFPWEVQ---VVAGEHDLFSTSGD--EQTIDVSDITYHEMFASHGTNYDVCLLKLKSSLHFNEKVKPIALPEKDQEFIGD---VVVSGWGTISSSGPASPVLKAVTVQVVSDEDCSDAY----YGSIDETMICAAAPGKXSCQGDSGGPL---AQDGTLVGIVSWGYGCAAPGYPGVYGKVSKFIDWI 232          

HSP 2 Score: 129.798 bits (325), Expect = 4.526e-34
Identity = 78/236 (33.05%), Postives = 122/236 (51.69%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSG-RQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNI-RREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            R+V G   + N  P+  +   +G   FCG S++D   I+TAAHC       +VQ++    GEH++    G+  T +  V+ +  H+ F+S     DV L+ LK+++    ++ ++P+ LP     + G +  V GWG++  +GP   VL+ +T+++  +  C   Y G     I + M+CA   GK SC GDSGGP           VGIVSWG GC    +PGVY + +    WI
Sbjct:   13 RIVGGEEVEPNSIPFQVSFQTTGGFHFCGASVMDKDTIITAAHCCDSFFPWEVQVVA---GEHDLFSTSGDEQTID--VSDITYHEMFASHGTNYDVCLLKLKSSL--HFNEKVKPIALPEKDQEFIGDV-VVSGWGTISSSGPASPVLKAVTVQVVSDEDCSDAYYG----SIDETMICAAAPGKXSCQGDSGGPL----AQDGTLVGIVSWGYGCAAPGYPGVYGKVSKFIDWI 232          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000004364 (supercontig:LSalAtl2s:LSalAtl2s229:72652:77339:1 gene:EMLSAG00000004364 transcript:EMLSAT00000004364 description:"augustus_masked-LSalAtl2s229-processed-gene-1.2")

HSP 1 Score: 145.591 bits (366), Expect = 6.561e-38
Identity = 87/267 (32.58%), Postives = 130/267 (48.69%), Query Frame = 0

Query:  668 EDVEAVQRIEGEHPLRVVNGWPADKNEWPW-----IAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESN-TFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVS-PAGIKDHMMCAGKK--GKDSCSGDSGGPFMIGNGDR--WEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            ED+ + +        R++ G PA    WPW     I A   S    CG  LI  +  +TAAHCV     +++ +   R+G++N   E E     +   ++++ H  ++     ND+AL+  K  VP      IR +CLP       G+ G V GWG + E GP+P +L E+ + I+ + RC  ++        + D  +CAG++  GKD+C GDSGGP +  +  R  W   GI SWG GCG+   PGVYTR      W+      F
Sbjct:  128 EDLTSRRLFNSRPQSRLIGGEPALFGAWPWQISLRIIASDESSNHRCGAVLISESWAITAAHCVHYYEMEEILL---RMGDYNRVEEDEPLPHMDRRPSKIIEHPKYNPRNFENDIALMHFKKPVP--FLPHIRSICLPEQGEDVVGKTGYVTGWGEIYEEGPRPRILHEVDIPIFGHKRCTDLFTKADLDELVTDVFICAGEEEGGKDACEGDSGGPLVFKSKKRGQWVLAGITSWGRGCGEKYQPGVYTRVPIYVEWVLETIKNF 389          

HSP 2 Score: 118.627 bits (296), Expect = 7.441e-29
Identity = 87/312 (27.88%), Postives = 150/312 (48.08%), Query Frame = 0

Query:  163 IFGICCVEKNIKADLLAE--IEALRTEE--PINPD-----EFPLDAQVNENCGKRSVGSNL--VRIVNGTEAKQHEFPFMAALF-----KNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAA-EQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTF---RDSCSGDSGGPL-FDCTDRCRQI--GIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            +FG CC  K  + +L+ E  +EA+  EE   + P       F       E+   R + ++    R++ G  A    +P+  +L      ++S   CG  L+ ++  +TAAHCV +    ++ ++ + +GD+N     E       R   ++ H ++      ND+A++   +   F P ++ ICL + G  +  G++  V GWG I E+G +  IL +VD+ ++ + +C   +       + T +   AG     +D+C GDSGGPL F    R + +  GI SWG GC     PGVYTRV   + W+ + +
Sbjct:   79 LFGSCCKLKFNQQNLIEENEVEAILNEEDSSLLPTATCGMRFNDFTTPEEDLTSRRLFNSRPQSRLIGGEPALFGAWPWQISLRIIASDESSNHRCGAVLISESWAITAAHCVHYY---EMEEILLRMGDYNRVEEDEPLPHMDRRPSKIIEHPKYNPRNFENDIALMHFKKPVPFLPHIRSICLPEQGE-DVVGKTGYVTGWGEIYEEGPRPRILHEVDIPIFGHKRCTDLFTKADLDELVTDVFICAGEEEGGKDACEGDSGGPLVFKSKKRGQWVLAGITSWGRGCGEKYQPGVYTRVPIYVEWVLETI 386          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000002836 (supercontig:LSalAtl2s:LSalAtl2s1638:13958:14826:-1 gene:EMLSAG00000002836 transcript:EMLSAT00000002836 description:"maker-LSalAtl2s1638-augustus-gene-0.2")

HSP 1 Score: 140.584 bits (353), Expect = 1.068e-37
Identity = 85/236 (36.02%), Postives = 128/236 (54.24%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAAL-FKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICL-EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G E + +  PF  ++       FCGGS++DK  I+TA HC  ++   D+    V  G+H+  +     EQ+  V  + RH +F T   + D+ +L L  +      V+P+ L E+D  F  +   V V+GWG++   GS S +LR V +++ S++ C  +Y     G     MICA    +DSC GDSGGPL   T     +GIVSWG GCA P +PG+Y +V++ + WI +
Sbjct:   26 RIVGGDEVEPNSIPFQISIEISGGYHFCGGSVMDKDTIITAGHCCTNLKPEDIL---VVAGEHDFLSM-SGDEQKIAVSEMXRHDKFGTYGPNYDICLLKLKSSLALNEKVKPVALPEKDQEFTGD---VVVSGWGTVEFGGSSSDVLRSVTLQLVSDEYCSDAY----DGHTDESMICAGSPGKDSCQGDSGGPL---TQDGTLVGIVSWGNGCAFPGYPGIYGKVSKFIDWIAE 247          

HSP 2 Score: 125.561 bits (314), Expect = 1.597e-32
Identity = 78/244 (31.97%), Postives = 124/244 (50.82%), Query Frame = 0

Query:  679 EHPLRVVNGWPADKNEWPW-IAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHN-IRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERAR 920
            +H  R+V G   + N  P+ I+  +  G  FCGGS++D   I+TA HC   +  +D+ ++    GEH+ +   G+    +  V+ + RH  F   T   +  +  LK     ++++ ++PV LP     +TG +  V GWG++   G   DVL+ +T+++  +  C   Y G +     + M+CAG  GKDSC GDSGGP           VGIVSWG GC    +PG+Y + +    WI   +
Sbjct:   22 KHSGRIVGGDEVEPNSIPFQISIEISGGYHFCGGSVMDKDTIITAGHCCTNLKPEDILVVA---GEHDFLSMSGDEQ--KIAVSEMXRHDKFG--TYGPNYDICLLKLKSSLALNEKVKPVALPEKDQEFTGDV-VVSGWGTVEFGGSSSDVLRSVTLQLVSDEYCSDAYDGHT----DESMICAGSPGKDSCQGDSGGPLT----QDGTLVGIVSWGNGCAFPGYPGIYGKVSKFIDWIAEQK 249          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|129688|sp|P21902.1|PCE_TACTR (RecName: Full=Proclotting enzyme; Contains: RecName: Full=Proclotting enzyme light chain; Contains: RecName: Full=Proclotting enzyme heavy chain; Flags: Precursor)

HSP 1 Score: 168.318 bits (425), Expect = 2.159e-44
Identity = 97/250 (38.80%), Postives = 140/250 (56.00%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAAL------LRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQ---MLTVRLGEHNIRR-EGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTG---RLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMI-GNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            R++ G  A    WPW+ A+      +RS +  CGG+L+ + H++TA+HCV   +  DV    + +VRLGEHN+   + +SN  +  VT V  H+ F   T  ND+A++TL + V  + +D IRP+CLP    RY     R   + GWG+   NGP   VL+E+ + IW++  C Q Y+      I +  MCAG    GKD+C GDSGGP M+      +  +GIVS+G  C    FPGVYT+ T+   WI
Sbjct:  127 RIIGGREAPIGAWPWMTAVYIKQGGIRSVQ--CGGALVTNRHVITASHCVVNSAGTDVMPADVFSVRLGEHNLYSTDDDSNPIDFAVTSVKHHEHFVLATYLNDIAILTLNDTV--TFTDRIRPICLPYRKLRYDDLAMRKPFITGWGTTAFNGPSSAVLREVQLPIWEHEACRQAYE--KDLNITNVYMCAGFADGGKDACQGDSGGPMMLPVKTGEFYLIGIVSFGKKCALPGFPGVYTKVTEFLDWI 370          

HSP 2 Score: 163.696 bits (413), Expect = 9.517e-43
Identity = 116/352 (32.95%), Postives = 181/352 (51.42%), Query Frame = 0

Query:  114 CVTNEGQNGTCTTVKECYPYFRLFNFDADETSTFGTYDTCSIDTRAGKKIFGICCVEK-NIKADLLAEIEALRTEEPINPDEFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQF----CGGSLLDKTHILTAAHCVEHMSQNDVRQMKVY---LGDHNL-RTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQ---VAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICA--AGTFRDSCSGDSGGPLF--DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            C     + GTC  V +C    +  +++  + S       C  +    K    +CC +  ++ +   A  E   TE P  P + P    + E CG  +  +   RI+ G EA    +P+M A++          CGG+L+   H++TA+HCV + +  DV    V+   LG+HNL  T  ++    F V +V  H+ F  +T  ND+A+LTL++   FT  ++PICL    L  Y+  +++   + GWG+ A +G  SA+LR+V + +W ++ CR++Y       IT   +CA  A   +D+C GDSGGP+     T     IGIVS+G  CA P FPGVYT+VTE + WI +
Sbjct:   40 CSNRFTEEGTCKNVLDCRILLQKNDYNLLKESI------CGFEGITPK----VCCPKSSHVISSTQAPPETTTTERP--PKQIP--PNLPEVCGIHNTTTT--RIIGGREAPIGAWPWMTAVYIKQGGIRSVQCGGALVTNRHVITASHCVVNSAGTDVMPADVFSVRLGEHNLYSTDDDSNPIDFAVTSVKHHEHFVLATYLNDIAILTLNDTVTFTDRIRPICLPYRKL-RYDDLAMRKPFITGWGTTAFNGPSSAVLREVQLPIWEHEACRQAYEKDL--NITNVYMCAGFADGGKDACQGDSGGPMMLPVKTGEFYLIGIVSFGKKCALPGFPGVYTKVTEFLDWIAE 372          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|18202734|sp|Q9BQR3.1|PRS27_HUMAN (RecName: Full=Serine protease 27; AltName: Full=Marapsin; AltName: Full=Pancreasin; Flags: Precursor)

HSP 1 Score: 160.229 bits (404), Expect = 1.359e-42
Identity = 99/257 (38.52%), Postives = 132/257 (51.36%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYN------DVALITLKNAVPESMSDTIRPVCLPSGSTRY-TGRLGTVVGWGSLRENG--PQPDVLQELTMEIWDNARCDQIYQ-----GVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            R+V G    + EWPW  ++ R+G  FCGGSLI    +LTAAHC    S  +  +  V LG   + + G       P     R +   S+ LY       DVAL+ L+  VP   ++ I PVCLP  S  + TG    V GWGS  E    P+P +LQ+L + I D  +C+ +Y      G  P  IK+ M+CAG  +  KD+C GDSGGP +   G  W Q G++SWG GC + + PGVY R T    WI R   K 
Sbjct:   34 RMVGGQDTQEGEWPWQVSIQRNGSHFCGGSLIAEQWVLTAAHCFRNTS--ETSLYQVLLGARQLVQPG-------PHAMYARVRQVESNPLYQGTASSADVALVELEAPVP--FTNYILPVCLPDPSVIFETGMNCWVTGWGSPSEEDLLPEPRILQKLAVPIIDTPKCNLLYSKDTEFGYQPKTIKNDMLCAGFEEGKKDACKGDSGGPLVCLVGQSWLQAGVISWGEGCARQNRPGVYIRVTAHHNWIHRIIPKL 279          

HSP 2 Score: 136.346 bits (342), Expect = 3.365e-34
Identity = 90/269 (33.46%), Postives = 134/269 (49.81%), Query Frame = 0

Query:  199 AQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDG--SQSAILRKVDVEVWSNDKCRRSYGNQA-----PGGITTHMICAAGT--FRDSCSGDSGGPLFDCTDRC-RQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIK 457
            A+    CG+  +   L R+V G + ++ E+P+  ++ +N   FCGGSL+ +  +LTAAHC  + S+  + Q  V LG   L   G  A    RV+ V  +  ++ +    DVA++ L     FT  + P+CL    +    G +  V GWGS +E+    +  IL+K+ V +    KC   Y         P  I   M+CA      +D+C GDSGGPL     +   Q G++SWG GCAR   PGVY RVT    WI +I+  L  +
Sbjct:   20 AKAATACGRPRM---LNRMVGGQDTQEGEWPWQVSIQRNGSHFCGGSLIAEQWVLTAAHCFRNTSETSLYQ--VLLGARQLVQPGPHA-MYARVRQVESNPLYQGTASSADVALVELEAPVPFTNYILPVCLPDPSVIFETGMNCWVTGWGSPSEEDLLPEPRILQKLAVPIIDTPKCNLLYSKDTEFGYQPKTIKNDMLCAGFEEGKKDACKGDSGGPLVCLVGQSWLQAGVISWGEGCARQNRPGVYIRVTAHHNWIHRIIPKLQFQ 282          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|78100736|sp|Q6BEA2.1|PRS27_RAT (RecName: Full=Serine protease 27; AltName: Full=Marapsin; AltName: Full=Pancreasin; Flags: Precursor)

HSP 1 Score: 160.999 bits (406), Expect = 2.156e-42
Identity = 96/246 (39.02%), Postives = 133/246 (54.07%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRY-TGRLGTVVGWGSLRENG--PQPDVLQELTMEIWDNARCDQIYQGVSPAGI-----KDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            R+V G  A + EWPW  ++ R+G  FCGGSLI  T +LTAAHC +  S  D+ +  V LG   +++ G    +  PV RV  H  +       DVAL+ L+  VP + +  I PVCLP  S  + +G    V GWGS  E    P P +LQ+L + + D  +C+ +Y   + A I     KD M+CAG  +  KD+C GDSGGP +      W Q G++SWG GC + + PGVY R      WI +
Sbjct:   37 RMVGGEDALEGEWPWQVSIQRNGAHFCGGSLIAPTWVLTAAHCFSNTS--DISIYQVLLGALKLQQPGPHALY-VPVKRVKSHPEYQGMASSADVALVELQ--VPVTFTKYILPVCLPDPSVVFKSGMNCWVTGWGSPSEQDRLPNPRILQKLAVPLIDTPKCNLLYSKDAEADIQLKTIKDDMLCAGFAEGKKDACKGDSGGPLVCLVDQSWVQAGVISWGEGCARRNRPGVYIRVASHYQWIHQ 277          

HSP 2 Score: 137.887 bits (346), Expect = 2.059e-34
Identity = 91/255 (35.69%), Postives = 126/255 (49.41%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGS--QSAILRKVDVEVWSNDKCRRSYGNQAPGGITTH-----MICA--AGTFRDSCSGDSGGPLFDCTDRCR-QIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIKAGA 460
            R+V G +A + E+P+  ++ +N   FCGGSL+  T +LTAAHC  + S  D+   +V LG   L+  G  A     VK V  H  ++      DVA++ L     FT  + P+CL    +    G +  V GWGS +E        IL+K+ V +    KC   Y   A   I        M+CA  A   +D+C GDSGGPL    D+   Q G++SWG GCAR   PGVY RV     WI +I+  L  +  A
Sbjct:   37 RMVGGEDALEGEWPWQVSIQRNGAHFCGGSLIAPTWVLTAAHCFSNTS--DISIYQVLLGALKLQQPGPHA-LYVPVKRVKSHPEYQGMASSADVALVELQVPVTFTKYILPVCLPDPSVVFKSGMNCWVTGWGSPSEQDRLPNPRILQKLAVPLIDTPKCNLLYSKDAEADIQLKTIKDDMLCAGFAEGKKDACKGDSGGPLVCLVDQSWVQAGVISWGEGCARRNRPGVYIRVASHYQWIHQIIPELQFQGRA 288          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|78100735|sp|Q8BJR6.1|PRS27_MOUSE (RecName: Full=Serine protease 27; AltName: Full=Marapsin; AltName: Full=Pancreasin; Flags: Precursor)

HSP 1 Score: 156.762 bits (395), Expect = 6.830e-41
Identity = 95/246 (38.62%), Postives = 132/246 (53.66%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRY-TGRLGTVVGWGSLRENG--PQPDVLQELTMEIWDNARCDQIYQ-----GVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            R+V G  A + EWPW  ++ R+G  FCGGSLI  T +LTAAHC +  S  D+ +  V LG   +++ G    +  PV +V  +  +       DVAL+ L+   P + ++ I PVCLP  S  + +G    V GWGS  E    P P VLQ+L + I D  +C+ +Y            IKD M+CAG  +  KD+C GDSGGP +      W Q G++SWG GC + + PGVY R T    WI +
Sbjct:   37 RMVGGENALEGEWPWQVSIQRNGIHFCGGSLIAPTWVLTAAHCFSNTS--DISIYQVLLGALKLQQPGPHALY-VPVKQVKSNPQYQGMASSADVALVELQG--PVTFTNYILPVCLPDPSVIFESGMNCWVTGWGSPSEQDRLPNPRVLQKLAVPIIDTPKCNLLYNKDVESDFQLKTIKDDMLCAGFAEGKKDACKGDSGGPLVCLVDQSWVQAGVISWGEGCARRNRPGVYIRVTSHHKWIHQ 277          

HSP 2 Score: 134.035 bits (336), Expect = 4.267e-33
Identity = 90/257 (35.02%), Postives = 127/257 (49.42%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGS--QSAILRKVDVEVWSNDKCRRSYGNQAPGG-----ITTHMICA--AGTFRDSCSGDSGGPLFDCTDRC-RQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIKAGADT 462
            R+V G  A + E+P+  ++ +N   FCGGSL+  T +LTAAHC  + S  D+   +V LG   L+  G  A     VK V  + +++      DVA++ L     FT  + P+CL    +    G +  V GWGS +E        +L+K+ V +    KC   Y            I   M+CA  A   +D+C GDSGGPL    D+   Q G++SWG GCAR   PGVY RVT    WI +I+  L  +  A T
Sbjct:   37 RMVGGENALEGEWPWQVSIQRNGIHFCGGSLIAPTWVLTAAHCFSNTS--DISIYQVLLGALKLQQPGPHA-LYVPVKQVKSNPQYQGMASSADVALVELQGPVTFTNYILPVCLPDPSVIFESGMNCWVTGWGSPSEQDRLPNPRVLQKLAVPIIDTPKCNLLYNKDVESDFQLKTIKDDMLCAGFAEGKKDACKGDSGGPLVCLVDQSWVQAGVISWGEGCARRNRPGVYIRVTSHHKWIHQIIPELQFQGRAGT 290          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|338817867|sp|Q8BZ10.2|TM11G_MOUSE (RecName: Full=Transmembrane protease serine 11G; AltName: Full=Serine protease DESC4; Contains: RecName: Full=Transmembrane protease serine 11G non-catalytic chain; Contains: RecName: Full=Transmembrane protease serine 11G catalytic chain; Flags: Precursor)

HSP 1 Score: 159.073 bits (401), Expect = 7.554e-41
Identity = 92/239 (38.49%), Postives = 135/239 (56.49%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVV-GWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG-KKGK-DSCSGDSGGPFMIG-NGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIE 917
            R+ +G PADK  WPW ++L   G   CG SLI S  ++T+AHC      ++ ++ TV  G     R   S      V  ++ H+ ++S    +D+A++ L +  P   S+ +  VCLP  + +   +    V GWG+L+ NGP P+ LQE+ +EI  N  C+Q+   V    I   M+CAG   GK D+C GDSGGP +I  N ++W  +GIVSWGI CGK + PG+YTR T  + WI+
Sbjct:  185 RIADGKPADKASWPWQSSLQVEGIHLCGASLIGSQWLVTSAHCFDNY--KNPKLWTVSFG-----RTLSSPLTTRKVESIIVHENYASHKHDDDIAVVKLSS--PVLFSENLHRVCLPDATFQVLPKSKVFVTGWGALKANGPFPNSLQEVEIEIISNDVCNQV--NVYGGAISSGMICAGFLTGKLDACEGDSGGPLVISDNRNKWYLLGIVSWGIDCGKENKPGIYTRVTHYRDWIK 412          

HSP 2 Score: 125.946 bits (315), Expect = 1.322e-29
Identity = 82/254 (32.28%), Postives = 134/254 (52.76%), Query Frame = 0

Query:  201 VNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRS-VQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICA---AGTFRDSCSGDSGGPLF--DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQ 448
            +N +CG       + RI +G  A +  +P+ ++L       CG SL+    ++T+AHC ++    + +   V  G    RT       + +V++++ H+ + +    +D+AV+ LS   +F+  +  +CL  D  F    +S V V GWG++  +G     L++V++E+ SND C +   N   G I++ MICA    G   D+C GDSGGPL   D  ++   +GIVSWGI C +   PG+YTRVT    WI+
Sbjct:  170 LNSDCGSGMEYPPIARIADGKPADKASWPWQSSLQVEGIHLCGASLIGSQWLVTSAHCFDNY--KNPKLWTVSFG----RTLSSPLTTR-KVESIIVHENYASHKHDDDIAVVKLSSPVLFSENLHRVCLP-DATFQVLPKSKVFVTGWGALKANGPFPNSLQEVEIEIISNDVCNQV--NVYGGAISSGMICAGFLTGKL-DACEGDSGGPLVISDNRNKWYLLGIVSWGIDCGKENKPGIYTRVTHYRDWIK 412          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|68051955|sp|Q5QSK2.1|TM11G_RAT (RecName: Full=Transmembrane protease serine 11G; AltName: Full=Serine protease DESC4; Contains: RecName: Full=Transmembrane protease serine 11G non-catalytic chain; Contains: RecName: Full=Transmembrane protease serine 11G catalytic chain; Flags: Precursor)

HSP 1 Score: 158.688 bits (400), Expect = 1.202e-40
Identity = 99/278 (35.61%), Postives = 147/278 (52.88%), Query Frame = 0

Query:  648 VSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVV-GWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG-KKGK-DSCSGDSGGPFMIG-NGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARN 921
            + +  CG+G  +P              RI      R+ +G PA  N WPW ++L   G   CG SLI S  ++T+AHC      ++ ++ TV  G    R  G   T    V  ++ H+ +++    +D+A++ L +  P   S+ +R VCLP  + +   +    V GWG+L+ NGP P+ LQE+ +EI  N  C+Q+   V    I   M+CAG   GK D+C GDSGGP +I  N ++W  +GIVSWGI CGK + PG+YTR T  + WI+   N
Sbjct:  169 ILNSNCGLGMEYP--------------RIA-----RIADGKPAGSNSWPWQSSLQVEGIHLCGASLIGSQWLVTSAHCFDNY--KNPKLWTVSFG----RTLGNPLT-TRKVESIIIHENYAAHKHDDDIAVVKLSS--PVLFSENLRTVCLPEATFQVLPKSKVFVTGWGALKANGPFPNSLQEVEIEIISNDVCNQV--NVYGGAISSGMICAGFLTGKLDACEGDSGGPLVISDNRNKWYLLGIVSWGIDCGKENKPGIYTRVTHYRNWIKSKTN 416          

HSP 2 Score: 129.413 bits (324), Expect = 7.867e-31
Identity = 86/285 (30.18%), Postives = 143/285 (50.18%), Query Frame = 0

Query:  169 VEKNIKADLLAEIEALRTEEPINPDEFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICA---AGTFRDSCSGDSGGPLF--DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQ 448
            ++++I    L E+ A + E  +N            NCG       + RI +G  A  + +P+ ++L       CG SL+    ++T+AHC ++    + +   V  G    RT G     + +V++++ H+ +      +D+AV+ LS   +F+  ++ +CL +          V V GWG++  +G     L++V++E+ SND C +   N   G I++ MICA    G   D+C GDSGGPL   D  ++   +GIVSWGI C +   PG+YTRVT    WI+
Sbjct:  149 LKRDISLPYLREMNAAQAEHILN-----------SNCGLGMEYPRIARIADGKPAGSNSWPWQSSLQVEGIHLCGASLIGSQWLVTSAHCFDNYK--NPKLWTVSFG----RTLGNPLTTR-KVESIIIHENYAAHKHDDDIAVVKLSSPVLFSENLRTVCLPEATFQVLPKSKVFVTGWGALKANGPFPNSLQEVEIEIISNDVCNQV--NVYGGAISSGMICAGFLTGKL-DACEGDSGGPLVISDNRNKWYLLGIVSWGIDCGKENKPGIYTRVTHYRNWIK 412          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|74835253|sp|Q27081.1|CFB_TACTR (RecName: Full=Clotting factor B; AltName: Full=Coagulation factor B; Contains: RecName: Full=Clotting factor B light chain; Contains: RecName: Full=Clotting factor B heavy chain; Flags: Precursor)

HSP 1 Score: 157.532 bits (397), Expect = 1.963e-40
Identity = 111/336 (33.04%), Postives = 161/336 (47.92%), Query Frame = 0

Query:  603 VHAPIMTTPTTTTTTTTTTVWTTTTAPTTPSHSPVGQGE-PVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRS----GRQFCGGSLIDSTHILTAAHCVAQMSAQDV-QMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTR---YTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVS----PAGIKDHMMCAGKK--GKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            V  P    P   TTT  T +  +T  P  P     G G+  V     + G  G     PIS  +N      +          +  G  A    WPW+AA+       GR  C GS+I + +IL+AAH       +     L VR+G H I+R G+    E PV  V+ H  +     YND+A+I LK  +  + +D + P+CLP   T       R+ T  GWG L  +GP+  VL+E+++ +    +CDQ Y+ ++      GI ++ +CAG +  GKD+C GDSGGP M+ N  RW  VG+VS+G  C +  +PGVY+R      WI +  N  
Sbjct:   78 VCCPDAIAPPPVTTTAVTVI--STKEPKLPRLHISGCGKRKVKIDITTVGRSGSPILPPISTPQNSTGGRGI----------IAGGVEAKIGAWPWMAAVFVKNFGIGRFHCAGSIISNKYILSAAHAFLIGGRKLTPTRLAVRVGGHYIKR-GQ----EYPVKDVIIHPHYVEKENYNDIAIIELKEEL--NFTDLVNPICLPDPETVTDPLKDRIVTAAGWGDLDFSGPRSQVLREVSIPVVPVDKCDQAYEKLNTPSLKNGITNNFLCAGLEEGGKDACQGDSGGPLMLVNNTRWIVVGVVSFGHKCAEEGYPGVYSRVASYLDWIAKVTNSL 394          

HSP 2 Score: 137.117 bits (344), Expect = 1.520e-33
Identity = 91/253 (35.97%), Postives = 132/253 (52.17%), Query Frame = 0

Query:  217 IVNGTEAKQHEFPFMAALF-KN---SRQFCGGSLLDKTHILTAAHCVEHMSQNDV-RQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFN--YEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGN----QAPGGITTHMICAAGTF--RDSCSGDSGGPLFDCTD-RCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLD 455
            I  G EAK   +P+MAA+F KN    R  C GS++   +IL+AAH      +     ++ V +G H ++ RG    Q++ VK V+ H  +     +ND+A++ L E   FT  V PICL          + R V  AGWG +   G +S +LR+V + V   DKC ++Y          GIT + +CA      +D+C GDSGGPL    + R   +G+VS+G  CA   +PGVY+RV   + WI K+   LD
Sbjct:  148 IAGGVEAKIGAWPWMAAVFVKNFGIGRFHCAGSIISNKYILSAAHAFLIGGRKLTPTRLAVRVGGHYIK-RG----QEYPVKDVIIHPHYVEKENYNDIAIIELKEELNFTDLVNPICLPDPETVTDPLKDRIVTAAGWGDLDFSGPRSQVLREVSIPVVPVDKCDQAYEKLNTPSLKNGITNNFLCAGLEEGGKDACQGDSGGPLMLVNNTRWIVVGVVSFGHKCAEEGYPGVYSRVASYLDWIAKVTNSLD 395          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|136433|sp|P19799.1|TRY1_XENLA (RecName: Full=Trypsin; Flags: Precursor)

HSP 1 Score: 151.754 bits (382), Expect = 4.390e-40
Identity = 94/238 (39.50%), Postives = 135/238 (56.72%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQ-PDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIE 917
            +++ G    K+  P+I +L  SG  FCGGSLI +  +++AAHC           + VRLGEHNI     +  F +  ++V+RH G++S TL ND+ LI L +  P S++  +  V LPSG +   G    + GWG+   NG   PD+LQ L   I  NA+C+  Y    P  I  +M+C G  + GKDSC GDSGGP ++ NG   +  G+VSWG GC   ++PGVYT+  +   WI+
Sbjct:   20 KIIGGATCAKSSVPYIVSL-NSGYHFCGGSLITNQWVVSAAHCYKAS-------IQVRLGEHNIALSEGTEQFISS-SKVIRHSGYNSYTLDNDIMLIKLSS--PASLNAAVNTVPLPSGCSA-AGTSCLISGWGNTLSNGSNYPDLLQCLNAPILTNAQCNSAY----PGEITANMICVGYMEGGKDSCQGDSGGP-VVCNG---QLQGVVSWGYGCAMRNYPGVYTKVCNYNAWIQ 237          

HSP 2 Score: 127.102 bits (318), Expect = 1.211e-31
Identity = 79/239 (33.05%), Postives = 124/239 (51.88%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSA-ILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGT--FRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            +I+ G    +   P++ +L  +   FCGGSL+    +++AAHC +         ++V LG+HN+    E  EQ      V+RH  + + TL ND+ ++ LS  A     V  + L      +  G S  ++GWG+   +GS    +L+ ++  + +N +C  +Y    PG IT +MIC       +DSC GDSGGP+  C  + +  G+VSWG GCA   +PGVYT+V     WIQ  +
Sbjct:   20 KIIGGATCAKSSVPYIVSL-NSGYHFCGGSLITNQWVVSAAHCYK-------ASIQVRLGEHNI-ALSEGTEQFISSSKVIRHSGYNSYTLDNDIMLIKLSSPASLNAAVNTVPLPSG--CSAAGTSCLISGWGNTLSNGSNYPDLLQCLNAPILTNAQCNSAY----PGEITANMICVGYMEGGKDSCQGDSGGPVV-CNGQLQ--GVVSWGYGCAMRNYPGVYTKVCNYNAWIQNTI 240          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|657341407|sp|B5U2W0.1|VSP_BOMIG (RecName: Full=Venom serine protease Bi-VSP; AltName: Full=Thrombin-like enzyme; Flags: Precursor)

HSP 1 Score: 154.451 bits (389), Expect = 8.548e-40
Identity = 91/243 (37.45%), Postives = 130/243 (53.50%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAAL-LRSGRQ-------FCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGE-SNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSG----STRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTT 910
            RVV G PA    WPWIAAL  R  R         CGGSLI S H+LTAAHC     A+  ++  VR+G+ N+ R  + ++  +  +   + H  + S    +D+A++ L   VP   S+ + P+CLP      +  +      V GWGSL  +GP  D L E+ + +  N  C   Y   + A + D ++CAG  + GKD+C GDSGGP M+     + Q+G+VS+G  C  A +PGVYTR T
Sbjct:  113 RVVGGKPAVLGAWPWIAALGFRYPRNPALEPLWKCGGSLISSRHVLTAAHC-----AEINELYVVRIGDLNLVRNDDGAHPVQIEIESKIIHPDYISGVTKHDIAILKLVEEVP--FSEYVYPICLPVEDNLRNNNFERYYPFVAGWGSLAHHGPGSDDLMEVQVPVISNTECKNSYARFAAAHVTDTVLCAGYTQGGKDACQGDSGGPLMLPKKFTFYQIGVVSYGHKCAAAGYPGVYTRVT 348          

HSP 2 Score: 151.369 bits (381), Expect = 9.522e-39
Identity = 118/362 (32.60%), Postives = 163/362 (45.03%), Query Frame = 0

Query:   98 LPQFQQPPVQAPLGNACVTNEGQNGTCTTVKECYPYFRLFNFDADETSTFGTYDTCSIDTRAGKKIFGICCVEKNIKADLLAEIEALRTEEPINPDEFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAAL-FKNSRQ-------FCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNL-RTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLE-QDGLFN--YEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTF--RDSCSGDSGGPLFDCTD-RCRQIGIVSWGIGCARPVFPGVYTRVTELM 444
            L  F  P V       C T   + G C  ++ C P   +        + F     C  +         +CC  +  + D    I      EP+ P           +CG  +V     R+V G  A    +P++AAL F+  R         CGGSL+   H+LTAAHC E      VR     +GD NL R    A   Q  +++ + H  + +    +D+A+L L E   F+  V PICL  +D L N  +E     VAGWGS+A  G  S  L +V V V SN +C+ SY   A   +T  ++CA  T   +D+C GDSGGPL         QIG+VS+G  CA   +PGVYTRVT  +
Sbjct:   13 LIAFLHPLVHVASAQECTTPNNKAGKCLGIRVCKPLLEMLQTQGHAAADFLRQSVCKYENNNPI----VCCPNEESRED--RGILVGNEYEPLRP----------PHCGFSNVSHT--RVVGGKPAVLGAWPWIAALGFRYPRNPALEPLWKCGGSLISSRHVLTAAHCAEINELYVVR-----IGDLNLVRNDDGAHPVQIEIESKIIHPDYISGVTKHDIAILKLVEEVPFSEYVYPICLPVEDNLRNNNFERYYPFVAGWGSLAHHGPGSDDLMEVQVPVISNTECKNSYARFAAAHVTDTVLCAGYTQGGKDACQGDSGGPLMLPKKFTFYQIGVVSYGHKCAAAGYPGVYTRVTSYL 351          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|317373502|sp|Q86T26.3|TM11B_HUMAN (RecName: Full=Transmembrane protease serine 11B; AltName: Full=Airway trypsin-like protease 5)

HSP 1 Score: 153.295 bits (386), Expect = 6.455e-39
Identity = 92/238 (38.66%), Postives = 129/238 (54.20%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVV-GWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKG--KDSCSGDSGGPFMIGNG-DRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            ++VNG  + +  WPW A++   GR +CG SLI S  +L+AAHC A+    + +  TV  G   I       T    V  ++ H+ +SS  L++D+AL+ L   V  S ++ IR +CLP    + +     VV GWG+L  NG  P +LQE  ++I DN  C+  Y       + D M+CAG      D+C  DSGGP    +  + W  VGIVSWG GCGK + PGVYTR T  + WI
Sbjct:  184 KIVNGKSSLEGAWPWQASMQWKGRHYCGASLISSRWLLSAAHCFAK--KNNSKDWTVNFG---IVVNKPYMT--RKVQNIIFHENYSSPGLHDDIALVQLAEEV--SFTEYIRKICLPEAKMKLSENDNVVVTGWGTLYMNGSFPVILQEDFLKIIDNKICNASY--AYSGFVTDTMLCAGFMSGEADACQNDSGGPLAYPDSRNIWHLVGIVSWGDGCGKKNKPGVYTRVTSYRNWI 410          

HSP 2 Score: 137.887 bits (346), Expect = 1.272e-33
Identity = 84/257 (32.68%), Postives = 135/257 (52.53%), Query Frame = 0

Query:  201 VNENCGKRSVGSNLV---RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLG---DHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTF--RDSCSGDSGGPLF--DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWI 447
            +  NC  R V ++++   +IVNG  + +  +P+ A++    R +CG SL+    +L+AAHC     +N+ +   V  G   +    TR        +V+ ++ H+ + +  LH+D+A++ L+E   FT  ++ ICL +  +   E  +V V GWG++  +GS   IL++  +++  N  C  SY     G +T  M+CA       D+C  DSGGPL   D  +    +GIVSWG GC +   PGVYTRVT    WI
Sbjct:  166 LTNNCCGRQVANSIITGNKIVNGKSSLEGAWPWQASMQWKGRHYCGASLISSRWLLSAAHCFA--KKNNSKDWTVNFGIVVNKPYMTR--------KVQNIIFHENYSSPGLHDDIALVQLAEEVSFTEYIRKICLPEAKMKLSENDNVVVTGWGTLYMNGSFPVILQEDFLKIIDNKICNASYA--YSGFVTDTMLCAGFMSGEADACQNDSGGPLAYPDSRNIWHLVGIVSWGDGCGKKNKPGVYTRVTSYRNWI 410          

BLAST of trypsin-1 precursor vs. nr
Match: gi|910346046|ref|XP_013176914.1| (PREDICTED: transmembrane protease serine 9-like isoform X3 [Papilio xuthus])

HSP 1 Score: 325.094 bits (832), Expect = 7.799e-98
Identity = 176/344 (51.16%), Postives = 217/344 (63.08%), Query Frame = 0

Query:  588 PGIPGV-VQPPPPSYPVHAPIM-------TTPTTTTT-TTTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            P IP +   PP  + P H P +       T PTT+   +T +T W T    TT  T P   P    +P G  +V S  CG+ ++ P +YG   E       ++ E   R+V G  AD NEWPWIAAL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF+  TLYNDVA++TL    P   S TIRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW N  C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:  143 PTIPPLPTHPPDHTAPTHPPSIVAGNVEPTRPTTSRPPSTASTTWGTKPPSTTKQTWPPAYPTQPTKPTGQPAVDS-SCGI-KNGPQTYGTAYE-------LQDEE--RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDK--PVKFSKTIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 473          

HSP 2 Score: 263.462 bits (672), Expect = 7.559e-75
Identity = 124/235 (52.77%), Postives = 161/235 (68.51%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH+ F   TL+NDVA+LTL +   F+ T++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  240 RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDKPVKFSKTIRPICLP-SGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 473          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1209699514|gb|OWR51010.1| (seminal fluid protein CSSFP022 [Danaus plexippus plexippus])

HSP 1 Score: 326.25 bits (835), Expect = 9.131e-98
Identity = 194/451 (43.02%), Postives = 247/451 (54.77%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGI--LGV-----PGAQGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVHPGIPGV-VQPPPPSYPVHAPIMTTPTTTTTTTT----TTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C          + GVCC T   G  P   Q P +  LGV     P +     +P+   P  P       P          P IP +   PP  + P H P +     TTT  +    +T W T    TT  T     P    +P G   V S  CG+ ++ P +YG   E ++           R+V G  A+ NEWPWI AL  +GRQFCGGSLID  H+LTAAHCVA M++ DV  LT RLG++NIR   E+   E  + RVVRH+GF   TLYNDVA++TL    P + +  IRPVCLP G+  Y+G + TV+GWGSLRE+GPQP +LQE+++ IW N  C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   92 GICKSFRDCYPLFKIVDLSGYDGWVMGHYDTCSFVN-RENSELFGVCC-TEPVGTPPQ--QEPDVQRLGVFRPPYPISMNNYQHPS---PLLPKWMNMNEPLHRQFFSQWPPTIPPLPTHPPDHTAPTHPPSIVAGIPTTTKPSNGLPSTTWGTKPPATTKQTWSPAYPTQPTKPTGQPGVDS-SCGI-KNGPQTYGSTYESLDEE---------RIVGGHNAELNEWPWIVALFNNGRQFCGGSLIDDRHVLTAAHCVAHMTSLDVARLTARLGDYNIRTNTETQHVERRIKRVVRHRGFDMRTLYNDVAVLTLDQ--PVTFTKNIRPVCLPGGARAYSGLIATVIGWGSLRESGPQPSILQEVSIPIWTNNECRLKYGSAAPGGIVDHMLCAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRITSFLPWIQK 522          

HSP 2 Score: 261.151 bits (666), Expect = 1.868e-73
Identity = 123/235 (52.34%), Postives = 161/235 (68.51%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A+ +E+P++ ALF N RQFCGGSL+D  H+LTAAHCV HM+  DV ++   LGD+N+RT  E    + R+K VVRH+ F   TL+NDVAVLTL +   FT  ++P+CL   G   Y G    V GWGS+ E G Q +IL++V + +W+N++CR  YG+ APGGI  HM+CA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTR+T  +PWIQK
Sbjct:  289 RIVGGHNAELNEWPWIVALFNNGRQFCGGSLIDDRHVLTAAHCVAHMTSLDVARLTARLGDYNIRTNTETQHVERRIKRVVRHRGFDMRTLYNDVAVLTLDQPVTFTKNIRPVCLP-GGARAYSGLIATVIGWGSLRESGPQPSILQEVSIPIWTNNECRLKYGSAAPGGIVDHMLCAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRITSFLPWIQK 522          

BLAST of trypsin-1 precursor vs. nr
Match: gi|909560122|ref|XP_013135139.1| (PREDICTED: transmembrane protease serine 9-like isoform X2 [Papilio polytes])

HSP 1 Score: 323.553 bits (828), Expect = 2.206e-97
Identity = 175/344 (50.87%), Postives = 217/344 (63.08%), Query Frame = 0

Query:  588 PGIPGV-VQPPPPSYPVHAPIM-------TTPTTTTT-TTTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            P IP +   PP  + P H P +       T PTT+   +T +T W T    TT  T P   P    +P G  +V S  CG+ ++ P +YG   E       ++ E   R+V G  AD NEWPWIAAL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF+  TLYNDVA++TL    P   S  IRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW NA C   Y   +P GI +HM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:  141 PTIPPLPTHPPDHTAPTHPPSIVAGNVEPTKPTTSRPPSTASTTWGTKPPSTTKQTWPPAYPTQPTKPAGQPAVDS-SCGI-KNGPQTYGTAYE-------LQDEE--RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDK--PVKFSKAIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNAECRLKYGSAAPGGIVEHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 471          

HSP 2 Score: 262.307 bits (669), Expect = 1.949e-74
Identity = 123/235 (52.34%), Postives = 160/235 (68.09%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH+ F   TL+NDVA+LTL +   F+  ++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  238 RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDKPVKFSKAIRPICLP-SGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNAECRLKYGSAAPGGIVEHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 471          

BLAST of trypsin-1 precursor vs. nr
Match: gi|913320391|ref|XP_013191309.1| (PREDICTED: proclotting enzyme isoform X2 [Amyelois transitella])

HSP 1 Score: 324.709 bits (831), Expect = 2.452e-97
Identity = 190/449 (42.32%), Postives = 238/449 (53.01%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNYQANTPS---WMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQ-------YPGILGVPGAQGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVHPGIPGVVQPPPPSYPVHAPIMTTPTTTTTTTTTTVWTT----TTAPTTPSHSPV--GQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C +   C+PLF   AN P    W+MG  D C          + GVCC T   G  P            P ++ +       ++P      +P    F             P IP +        P H P  T PT   +  TTT W T    TT  T P   P    + +PV      S ECG+         +NG    A Q  + +   R+V G  AD NEWPWI AL  +GRQFCGGSLID  H+L+AAHCVA M++ DV  LT RLG++NI    E+   E  + RVVRH+GF   TLYNDVA++TL   VP   +  + P+CLP     Y G + TV+GWGSLRE+GPQP +LQE+++ IW NA C Q Y   +P GI +HM+CAGK   DSCSGDSGGP M+  G RW QVGIVSWGIGCGK  +PGVYTR T    WI++
Sbjct:   89 GTCKSFHDCYPLFKV-ANLPGLDGWVMGHYDTCSYIS-GDNMEVFGVCC-TEPVGTPPQQEPDVQRLGILPPVMPMQLPPVNSMFPNILSALAPQWPQFQNVNIRQIPANWPPTIPPL--------PTHPPDHTAPTHPPSLGTTTTWATKPSSTTKQTWPPAFPTQPAKPQPVKPSPQPSTECGI---------KNG----AHQTYDLQDEERIVGGHNADLNEWPWIVALFNNGRQFCGGSLIDDRHVLSAAHCVAHMTSWDVARLTARLGDYNIHSNVETQHIERKIKRVVRHRGFDMRTLYNDVAVLTLDQPVP--FTKKVHPICLPGSGKAYAGIVATVIGWGSLRESGPQPSILQEVSIPIWQNAECRQKYGAAAPGGIVEHMLCAGKASMDSCSGDSGGPLMVNEGGRWTQVGIVSWGIGCGKGQYPGVYTRVTAFLPWIQK 511          

HSP 2 Score: 253.832 bits (647), Expect = 7.116e-71
Identity = 128/269 (47.58%), Postives = 165/269 (61.34%), Query Frame = 0

Query:  188 EPINPDEFPLDAQVNENCGKRSVGSNLV------RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            +P  P       Q +  CG ++            RIV G  A  +E+P++ ALF N RQFCGGSL+D  H+L+AAHCV HM+  DV ++   LGD+N+ +  E    + ++K VVRH+ F   TL+NDVAVLTL +   FT  V PICL   G   Y G    V GWGS+ E G Q +IL++V + +W N +CR+ YG  APGGI  HM+CA     DSCSGDSGGPL  +   R  Q+GIVSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  244 QPAKPQPVKPSPQPSTECGIKNGAHQTYDLQDEERIVGGHNADLNEWPWIVALFNNGRQFCGGSLIDDRHVLSAAHCVAHMTSWDVARLTARLGDYNIHSNVETQHIERKIKRVVRHRGFDMRTLYNDVAVLTLDQPVPFTKKVHPICLPGSGK-AYAGIVATVIGWGSLRESGPQPSILQEVSIPIWQNAECRQKYGAAAPGGIVEHMLCAGKASMDSCSGDSGGPLMVNEGGRWTQVGIVSWGIGCGKGQYPGVYTRVTAFLPWIQK 511          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1344767871|ref|XP_023945684.1| (transmembrane protease serine 9 isoform X1 [Bicyclus anynana])

HSP 1 Score: 323.939 bits (829), Expect = 1.052e-96
Identity = 202/480 (42.08%), Postives = 250/480 (52.08%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGV----------------------------------HPG--IPGVVQPPPPSYPVHAPIMTTPTTTTTT----TTTTVW-----TTTTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C          + GVCC T+  G  P   Q P +      + G ++P  + P SP       PG   S +                                    HP   + GV QPP       AP  T PTTT  T    TT   W     T    PT P+  P     PVG  +     CGV ++ P  YG   E     +RI G H         A+ NEWPWI AL   GRQFCGGSLID++H+L+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF   TLYNDV+++TL    P + S TIRP+CLP GS  Y+G   TV+GWGSLRE+GPQP +LQE+++ IW N  C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVGIVSWGIGCGK  +PGVYTR T    WI++
Sbjct:   91 GTCKSFRDCYPLFKVVDLSGYDGWVMGHYDTCSYIN-NDNTEVFGVCC-TQPVGTPPQ--QEPDV-----QRFGVLHP--SFPLSPLSS---FPGLPPSALAAQFAYNNQLTRQIPAVWPPTLPPLPTHPPDHTAPTHPPSLVAGVTQPP------QAPASTPPTTTWGTKPPSTTKQTWPPAYPTQPARPTAPTQPPRPTA-PVGVDT----SCGV-KNGPQVYGATAESSTDEERIVGGHN--------AELNEWPWIVALFNGGRQFCGGSLIDNSHVLSAAHCVAHMTSWDVARLTARLGDHNIRTNSETQHVERKIKRVVRHRGFDMRTLYNDVSVLTLDQ--PVTYSKTIRPICLPGGSRAYSGLTATVIGWGSLRESGPQPSILQEVSIPIWTNNECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMLNEGGRWTQVGIVSWGIGCGKGQYPGVYTRVTAFLPWIQK 534          

HSP 2 Score: 263.077 bits (671), Expect = 4.402e-74
Identity = 123/235 (52.34%), Postives = 163/235 (69.36%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPL-FDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A+ +E+P++ ALF   RQFCGGSL+D +H+L+AAHCV HM+  DV ++   LGDHN+RT  E    + ++K VVRH+ F   TL+NDV+VLTL +   ++ T++PICL   G   Y G +  V GWGS+ E G Q +IL++V + +W+N++CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+GIVSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  301 RIVGGHNAELNEWPWIVALFNGGRQFCGGSLIDNSHVLSAAHCVAHMTSWDVARLTARLGDHNIRTNSETQHVERKIKRVVRHRGFDMRTLYNDVSVLTLDQPVTYSKTIRPICLP-GGSRAYSGLTATVIGWGSLRESGPQPSILQEVSIPIWTNNECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMLNEGGRWTQVGIVSWGIGCGKGQYPGVYTRVTAFLPWIQK 534          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1285017473|ref|XP_023020913.1| (proclotting enzyme [Leptinotarsa decemlineata])

HSP 1 Score: 321.627 bits (823), Expect = 3.916e-96
Identity = 196/459 (42.70%), Postives = 241/459 (52.51%), Query Frame = 0

Query:  484 VMGHCVTLKSCFPLFNYQ--ANTPSWMMGEKDMCQTTEPTTRQSMAGVCCS----------------TRQFGQYPLYPQYPGILGVPGAQGGGIYPAGT----IPFSPGQGGFGTPGFGGSGIGVHPGIPGVVQPPPPSYPVHAPIMTT--PTTTTTTTTTTVWTTTTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            V+G CV+ + C+P F      N  SW++G  D C       RQ M GVCC+                T++       P Y     +        YP       +P                  G +P IP    PP    PV+ P   T  PT   T    TV TTT+AP                   S+  CG    +P        D E           R+V G  AD NEWPWIAAL   GRQFCGGSLID THIL+AAHCVA MS+ DV  LTVRLG+HNI+   E+   E  V RVVRH+GF S TLYND+A++TL +  P   +  IRPVCLPSG + ++G+ GTV+GWGSLRE+GPQP VLQE+ + IW N  C   Y   +P GI +HM+CAG+  +DSCSGDSGGP MI  G +W QVGIVSWGIGCGK  +PGVYTR      WI++
Sbjct:   88 VLGKCVSFRQCYPYFKIPELGNFDSWILGMYDTCSYYTAQGRQ-MFGVCCTNPNPSKPTEATDSSSTTKEPNIESEGPDYTVEADLNSTAKNPYYPLSNWPPPLPTHSPDNTIPPLPTHPPSPG-YPTIPPTAWPPT-FKPVNKPGKPTVKPTEKPTIKPPTVGTTTSAPV---------------NIPSTASCGAKNGYP--------DQE-----------RIVGGHNADVNEWPWIAALFNGGRQFCGGSLIDKTHILSAAHCVAHMSSWDVARLTVRLGDHNIKTNSETRHVEKRVKRVVRHRGFDSRTLYNDIAILTLDS--PIEFTQQIRPVCLPSGGSDHSGKTGTVIGWGSLRESGPQPAVLQEVNIPIWTNRECKVKYGPAAPGGIVEHMLCAGQANRDSCSGDSGGPLMINTG-KWTQVGIVSWGIGCGKGQYPGVYTRVAKFLPWIQK 506          

HSP 2 Score: 276.174 bits (705), Expect = 3.156e-79
Identity = 130/256 (50.78%), Postives = 176/256 (68.75%), Query Frame = 0

Query:  196 PLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            P++     +CG ++   +  RIV G  A  +E+P++AALF   RQFCGGSL+DKTHIL+AAHCV HMS  DV ++ V LGDHN++T  E    + RVK VVRH+ F + TL+ND+A+LTL     FT  ++P+CL   G  ++ G++  V GWGS+ E G Q A+L++V++ +W+N +C+  YG  APGGI  HM+CA    RDSCSGDSGGPL   T +  Q+GIVSWGIGC +  +PGVYTRV + +PWIQK L
Sbjct:  254 PVNIPSTASCGAKNGYPDQERIVGGHNADVNEWPWIAALFNGGRQFCGGSLIDKTHILSAAHCVAHMSSWDVARLTVRLGDHNIKTNSETRHVEKRVKRVVRHRGFDSRTLYNDIAILTLDSPIEFTQQIRPVCLPSGGS-DHSGKTGTVIGWGSLRESGPQPAVLQEVNIPIWTNRECKVKYGPAAPGGIVEHMLCAGQANRDSCSGDSGGPLMINTGKWTQVGIVSWGIGCGKGQYPGVYTRVAKFLPWIQKNL 508          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1080057676|ref|XP_018570644.1| (proclotting enzyme-like isoform X2 [Anoplophora glabripennis])

HSP 1 Score: 320.857 bits (821), Expect = 4.591e-96
Identity = 188/438 (42.92%), Postives = 236/438 (53.88%), Query Frame = 0

Query:  484 VMGHCVTLKSCFPLFNYQ--ANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGA-QGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVHPGIPGVVQPPPPSYPVHAPIMTTPTTTTTTTTTTVWTTTTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            V+G C + + C+P F     +N  +W++G  D C       RQ M GVCC+T      P  P        P   Q  G       P+ P              I   P  P         +P  +P +  PT    TT         A T PS+ P     P  +  V+ G CG    +        +D E           R+V G  AD NEWPWIAAL   GRQFCGGSLID+ HIL+AAHCVA MS+ DV  LTV+LG+HNIR   E N  E  V RVVRH+GF S TLYND+A++TL +  P   +  IRPVCLP+G   ++G+  TV+GWGSLRE+GPQP +LQE+ + IW N  C   Y   +P GI +HM+CAG+  +DSCSGDSGGP M+ NG +W QVGIVSWGIGCGK  +PGVYTR      WI +
Sbjct:   87 VLGKCTSFRQCYPYFKMPEVSNFDNWVLGMYDTCSYFTSQGRQ-MFGVCCAT---SPKPTSPADSASTEEPTVEQAEGQNELSKKPYYPSWPPPLPTHPPDHTIPPLPTHPPSP-----GFPTQSPGVWPPTIKPITTKP---PKKPATTKPSYKPPSAKPPTVAIPVTGGSCGAKNGY--------QDQE-----------RIVGGHNADVNEWPWIAALFNGGRQFCGGSLIDNIHILSAAHCVAHMSSWDVARLTVKLGDHNIRTNSEINHVEKRVKRVVRHRGFDSRTLYNDIAILTLDS--PVDFTQQIRPVCLPNGGNDHSGKTATVIGWGSLRESGPQPAILQEVNIPIWSNRDCKLKYGPAAPGGIVEHMLCAGQANRDSCSGDSGGPLMVNNG-KWTQVGIVSWGIGCGKGQYPGVYTRVEKFLPWIYK 490          

HSP 2 Score: 270.011 bits (689), Expect = 3.987e-77
Identity = 128/248 (51.61%), Postives = 168/248 (67.74%), Query Frame = 0

Query:  204 NCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            +CG ++   +  RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HMS  DV ++ V LGDHN+RT  E    + RVK VVRH+ F + TL+ND+A+LTL     FT  ++P+CL   G  ++ G++  V GWGS+ E G Q AIL++V++ +WSN  C+  YG  APGGI  HM+CA    RDSCSGDSGGPL     +  Q+GIVSWGIGC +  +PGVYTRV + +PWI K L
Sbjct:  246 SCGAKNGYQDQERIVGGHNADVNEWPWIAALFNGGRQFCGGSLIDNIHILSAAHCVAHMSSWDVARLTVKLGDHNIRTNSEINHVEKRVKRVVRHRGFDSRTLYNDIAILTLDSPVDFTQQIRPVCLPNGG-NDHSGKTATVIGWGSLRESGPQPAILQEVNIPIWSNRDCKLKYGPAAPGGIVEHMLCAGQANRDSCSGDSGGPLMVNNGKWTQVGIVSWGIGCGKGQYPGVYTRVEKFLPWIYKNL 492          

HSP 3 Score: 64.3142 bits (155), Expect = 6.583e-7
Identity = 44/156 (28.21%), Postives = 66/156 (42.31%), Query Frame = 0

Query:   34 IVLEVTEDDLTSSGGQKLERSDNPFVSSESRRARQFPFSAPGVPFLQPVGGLPLQPSFVPARSNLPQFQQPPVQAPLGNACVTNEGQNGTCTTVKECYPYFRLFNFDADETSTFGTYDTCSIDTRAGKKIFGICCVEKNIKADLLAEIEALRTEEP 189
            ++     DD      Q +    +P     +R  RQF   +P +P   P          VP   N         QA  G  C T++G  G CT+ ++CYPYF++      +    G YDTCS  T  G+++FG+CC          +  ++  TEEP
Sbjct:   13 VIATTLADDPRDDDPQSVTIEADP-SEDHNRDGRQFVVRSPTLPVYNPF--------LVPQIWNNVNVNN--RQATTG-PCFTSKGVLGKCTSFRQCYPYFKMPEVSNFDNWVLGMYDTCSYFTSQGRQMFGVCCA---TSPKPTSPADSASTEEP 153          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1080057674|ref|XP_018570643.1| (proclotting enzyme-like isoform X1 [Anoplophora glabripennis])

HSP 1 Score: 320.857 bits (821), Expect = 4.878e-96
Identity = 188/438 (42.92%), Postives = 236/438 (53.88%), Query Frame = 0

Query:  484 VMGHCVTLKSCFPLFNYQ--ANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGA-QGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVHPGIPGVVQPPPPSYPVHAPIMTTPTTTTTTTTTTVWTTTTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            V+G C + + C+P F     +N  +W++G  D C       RQ M GVCC+T      P  P        P   Q  G       P+ P              I   P  P         +P  +P +  PT    TT         A T PS+ P     P  +  V+ G CG    +        +D E           R+V G  AD NEWPWIAAL   GRQFCGGSLID+ HIL+AAHCVA MS+ DV  LTV+LG+HNIR   E N  E  V RVVRH+GF S TLYND+A++TL +  P   +  IRPVCLP+G   ++G+  TV+GWGSLRE+GPQP +LQE+ + IW N  C   Y   +P GI +HM+CAG+  +DSCSGDSGGP M+ NG +W QVGIVSWGIGCGK  +PGVYTR      WI +
Sbjct:   88 VLGKCTSFRQCYPYFKMPEVSNFDNWVLGMYDTCSYFTSQGRQ-MFGVCCAT---SPKPTSPADSASTEEPTVEQAEGQNELSKKPYYPSWPPPLPTHPPDHTIPPLPTHPPSP-----GFPTQSPGVWPPTIKPITTKP---PKKPATTKPSYKPPSAKPPTVAIPVTGGSCGAKNGY--------QDQE-----------RIVGGHNADVNEWPWIAALFNGGRQFCGGSLIDNIHILSAAHCVAHMSSWDVARLTVKLGDHNIRTNSEINHVEKRVKRVVRHRGFDSRTLYNDIAILTLDS--PVDFTQQIRPVCLPNGGNDHSGKTATVIGWGSLRESGPQPAILQEVNIPIWSNRDCKLKYGPAAPGGIVEHMLCAGQANRDSCSGDSGGPLMVNNG-KWTQVGIVSWGIGCGKGQYPGVYTRVEKFLPWIYK 491          

HSP 2 Score: 270.011 bits (689), Expect = 4.445e-77
Identity = 128/248 (51.61%), Postives = 168/248 (67.74%), Query Frame = 0

Query:  204 NCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            +CG ++   +  RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HMS  DV ++ V LGDHN+RT  E    + RVK VVRH+ F + TL+ND+A+LTL     FT  ++P+CL   G  ++ G++  V GWGS+ E G Q AIL++V++ +WSN  C+  YG  APGGI  HM+CA    RDSCSGDSGGPL     +  Q+GIVSWGIGC +  +PGVYTRV + +PWI K L
Sbjct:  247 SCGAKNGYQDQERIVGGHNADVNEWPWIAALFNGGRQFCGGSLIDNIHILSAAHCVAHMSSWDVARLTVKLGDHNIRTNSEINHVEKRVKRVVRHRGFDSRTLYNDIAILTLDSPVDFTQQIRPVCLPNGG-NDHSGKTATVIGWGSLRESGPQPAILQEVNIPIWSNRDCKLKYGPAAPGGIVEHMLCAGQANRDSCSGDSGGPLMVNNGKWTQVGIVSWGIGCGKGQYPGVYTRVEKFLPWIYKNL 493          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1344767873|ref|XP_023945692.1| (chymotrypsin-like protease CTRL-1 isoform X2 [Bicyclus anynana])

HSP 1 Score: 319.701 bits (818), Expect = 8.021e-96
Identity = 172/338 (50.89%), Postives = 207/338 (61.24%), Query Frame = 0

Query:  590 IPGVVQPPPPSYPVHAPIMTTPTTTTTT----TTTTVW-----TTTTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            + GV QPP       AP  T PTTT  T    TT   W     T    PT P+  P     PVG  +     CGV ++ P  YG   E     +RI G H         A+ NEWPWI AL   GRQFCGGSLID++H+L+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF   TLYNDV+++TL    P + S TIRP+CLP GS  Y+G   TV+GWGSLRE+GPQP +LQE+++ IW N  C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVGIVSWGIGCGK  +PGVYTR T    WI++
Sbjct:  161 VAGVTQPP------QAPASTPPTTTWGTKPPSTTKQTWPPAYPTQPARPTAPTQPPRPTA-PVGVDT----SCGV-KNGPQVYGATAESSTDEERIVGGHN--------AELNEWPWIVALFNGGRQFCGGSLIDNSHVLSAAHCVAHMTSWDVARLTARLGDHNIRTNSETQHVERKIKRVVRHRGFDMRTLYNDVSVLTLDQ--PVTYSKTIRPICLPGGSRAYSGLTATVIGWGSLRESGPQPSILQEVSIPIWTNNECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMLNEGGRWTQVGIVSWGIGCGKGQYPGVYTRVTAFLPWIQK 476          

HSP 2 Score: 264.233 bits (674), Expect = 4.024e-75
Identity = 123/235 (52.34%), Postives = 163/235 (69.36%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPL-FDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A+ +E+P++ ALF   RQFCGGSL+D +H+L+AAHCV HM+  DV ++   LGDHN+RT  E    + ++K VVRH+ F   TL+NDV+VLTL +   ++ T++PICL   G   Y G +  V GWGS+ E G Q +IL++V + +W+N++CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+GIVSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  243 RIVGGHNAELNEWPWIVALFNGGRQFCGGSLIDNSHVLSAAHCVAHMTSWDVARLTARLGDHNIRTNSETQHVERKIKRVVRHRGFDMRTLYNDVSVLTLDQPVTYSKTIRPICLP-GGSRAYSGLTATVIGWGSLRESGPQPSILQEVSIPIWTNNECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMLNEGGRWTQVGIVSWGIGCGKGQYPGVYTRVTAFLPWIQK 476          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1247043863|gb|PCG78401.1| (hypothetical protein B5V51_4331 [Heliothis virescens])

HSP 1 Score: 318.546 bits (815), Expect = 9.969e-95
Identity = 190/465 (40.86%), Postives = 251/465 (53.98%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNYQ--ANTPSWMMGEKDMCQTTEPTTRQSMAGVCCS------------TRQFGQY-PLYP-QYPGILGVPGAQGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVHPGIPGV-VQPPPPSYPVHAPIM----TTPTTTTTTTTTTVWTT--------TTAPTTPSHS--PVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLP-SGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C        + + GVCC+             ++ G   P  P Q P   G+PG    G+  A    FS       +  F        P IP +   PP  + P H P +    TT   +    ++T W T        T AP  P+    P    +P   GS     CG+ ++ P +Y    +D E           R+V G  A+ NEWPWI AL  +GRQFCGGSL+D  H+L+AAHCVA M++ DV  LT RLG++NIR   E+   E  + RVVRH+GF   TLYNDVA++T+    P S +  IRP+CLP +G + Y G++ TV+GWGSLRE+GPQP VLQE+++ +W N  C   Y G +P GI +HM+CAGK   DSCSGDSGGP M+    RW QVGIVSWGIGCGK  +PGVYTR T    WI++
Sbjct:   90 GTCKSFRDCYPLFKVADFSGWDGWVMGHYDTCSYINGENME-VFGVCCTEPVGTPPQQEPDVQRLGYLQPAIPVQLPAFAGIPG----GVLSA----FSRWSYNGDSNNFRQIPAQWPPTIPPLPTHPPDHTAPTHPPSLVAGVTTKPPSNVPASSTTWATRPPSTTKQTWAPAYPTKPSVPAQPAQPAVDGS-----CGI-KNGPQTYESTYQDEE-----------RIVGGHNAELNEWPWIVALFNAGRQFCGGSLMDDRHVLSAAHCVAHMTSWDVARLTARLGDYNIRTNTETQHIERKIKRVVRHRGFDMRTLYNDVAVLTIDQ--PVSFTKNIRPICLPVAGRSTYQGKVATVIGWGSLRESGPQPSVLQEVSIPVWSNQECKLKYGGAAPGGIVEHMLCAGKASMDSCSGDSGGPLMVNENGRWTQVGIVSWGIGCGKGQYPGVYTRVTYFLPWIQK 526          

HSP 2 Score: 263.077 bits (671), Expect = 3.940e-74
Identity = 127/262 (48.47%), Postives = 169/262 (64.50%), Query Frame = 0

Query:  196 PLDAQVNENCGKR-------SVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            P    V+ +CG +       S   +  RIV G  A+ +E+P++ ALF   RQFCGGSL+D  H+L+AAHCV HM+  DV ++   LGD+N+RT  E    + ++K VVRH+ F   TL+NDVAVLT+ +   FT  ++PICL   G   Y+G+   V GWGS+ E G Q ++L++V + VWSN +C+  YG  APGGI  HM+CA     DSCSGDSGGPL  +   R  Q+GIVSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  265 PAQPAVDGSCGIKNGPQTYESTYQDEERIVGGHNAELNEWPWIVALFNAGRQFCGGSLMDDRHVLSAAHCVAHMTSWDVARLTARLGDYNIRTNTETQHIERKIKRVVRHRGFDMRTLYNDVAVLTIDQPVSFTKNIRPICLPVAGRSTYQGKVATVIGWGSLRESGPQPSVLQEVSIPVWSNQECKLKYGGAAPGGIVEHMLCAGKASMDSCSGDSGGPLMVNENGRWTQVGIVSWGIGCGKGQYPGVYTRVTYFLPWIQK 526