BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000011454 (supercontig:LSalAtl2s:LSalAtl2s790:183804:208736:-1 gene:EMLSAG00000011454 transcript:EMLSAT00000011454 description:"maker-LSalAtl2s790-augustus-gene-2.32")

HSP 1 Score: 607.831 bits (1566), Expect = 0.000e+0
Identity = 325/725 (44.83%), Postives = 430/725 (59.31%), Query Frame = 0

Query:  202 NENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIKAGADTLVNSIGPGPKSTPSHWAGGTPVMGHCVTLKSCFPLFNYQANTPSWMMGEKDMCQTTEPTTRQSMA-GVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVHPGIPGVVQPPPPSYPVHAPIMTTPTTTTTT-TTTTVWTTTTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRY-TGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            +++CG+R   +NL +IVNG +A   EFPFMAALF   R F GGS++D  HILTAAHCV HM++ DVR ++V++  + +++     EQ+FRV+ V+RHK F  +TL+NDVA+LTL  +  F+ +VQPICL  D    YEG  V VAGWG++++ G QS+IL+KV V VWSN  C  SYG++APGGI +HM+CAA   +DSCSGDSGGPLF C   C QIGIVSWGIG      P  Y                 D  +G  + +      P ++ +  +G       C++ KSC+P    +++ P W+ G+ ++C+   P   Q     +CC          YP               IYP   I + PG  G                 P + +          P++T+ TT+     T +  TT+T+ TT          P          CG   H+PI Y ENG   E + RI+ EH L++VNGWPA K+EWPWIAAL   GRQFCGGSLI  +HILTAAHCVAQM+  DV  L VRLGEHNI+   ++      V +++RHKGFSS TL+NDVAL+ L +  P S SD I+P+C+ +    Y      TVVGWGSLRE+GPQP +LQE+T+ +W N +C + Y G SPAGI DHM+CAG++GKDSCSGDSGGP +   GD+W Q+GIVSWGIGCGK+ FPGVYTR   +  WI +   KF
Sbjct:  238 SDHCGQRFFSNNL-KIVNGEDASVDEFPFMAALFNKXRHFXGGSIIDSKHILTAAHCVAHMTKIDVRYLRVHVRQYRIKSSNIGTEQEFRVRRVIRHKSFSANTLYNDVAILTLRGSIDFSKSVQPICLASDNTETYEGDIVTVAGWGTLSDGGRQSSILQKVSVGVWSNYDCGASYGSRAPGGIKSHMLCAASKGKDSCSGDSGGPLFYCDTYCTQIGIVSWGIGSK----PDSYDD---------------DHPSGQSSNIYQHSKFPCTSSNGRSG------RCISFKSCYPFHRVRSDVPEWVFGDANVCKNRNPREAQDQRREICCENVYQSILGFYP---------------IYPVHVI-YVPGVDG-----------------PILTETISGXDXQFLPVITSTTTSXLRPITISTDTTSTSTTTIVKETTTTSRPDNLVINDQSVCGNSIHYPIVYDENGSVSEEINRIDMEHDLKIVNGWPASKHEWPWIAALFNRGRQFCGGSLISRSHILTAAHCVAQMTRSDVNNLEVRLGEHNIKDALDTQVEVRKVNKIIRHKGFSSQTLWNDVALLFLSD--PVSYSDIIKPICIDTSDDPYDKNXTATVVGWGSLRESGPQPXILQEVTLRLWKNEKCQETYNGASPAGITDHMICAGRQGKDSCSGDSGGPMVKAKGDKWVQIGIVSWGIGCGKSHFPGVYTRVGKMYEWITKVIEKF 901          

HSP 2 Score: 266.159 bits (679), Expect = 4.429e-76
Identity = 184/592 (31.08%), Postives = 279/592 (47.13%), Query Frame = 0

Query:   28 FIDGDAIVLEVTEDDLTSSGGQKLERSDNPFVSSESRRARQFPFSAPGVPFLQPVGGLPLQPSFVPARSNLPQFQQPPVQAPLGNACVTNEGQNGTCTTVKECYPYFRLFNFDADETSTFGTYDTCSIDTRAGKKIFGICCVEKNIKADLLAEIEALRTEEPINPDEFPLDAQVNEN------------------CGKRSVGSNLVRIVNGTEAKQHEFPFMA-----------------ALFKNSRQFCGG------------SLLDKTH------ILTAAH------CVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIKAGADTLVNSIGPGPKSTPSHWAGGTPVMGHCVTLKSCFPLFNYQANTPSWMMGEKDMCQTTEPTTRQSM-AGVCCSTRQ---FGQYPLYPQYPGILGVPGAQG 556
            F D D++V+E   DD+     + ++R D  F     R  RQ P     +PF        L  S   + +N+ Q             C+T+ G+ G CT++K CYPYF+  N     T   G YDTCS   +  +++FG+CC    + A+ + +  +    +P  P  FP+  Q + N                    +++       I+ G E ++ E                        F N+ +   G            +L +K        I+ + H      CV HM++ DVR ++V++  + +++     EQ+FRV+ V+RHK F  +TL+NDVA+LTL  +  F+ +VQPICL  D    YEG  V VAGWG++++ G QS+IL+KV V VWSN  C  SYG++APGGI +HM+CAA   +DSCSGDSGGPLF C   C QIGIVSWGIG      P  Y                 D  +G  + +      P ++ +  +      G C++ KSC+P    +++ P W+ G+ ++C+   P   Q     +CC        G YP+YP +  ++ VPG  G
Sbjct:   40 FGDEDSVVIE---DDID----RHVDREDLGF----DRNDRQLP----NIPF-------DLNSSKKNSGANIYQ------------PCLTSTGRLGRCTSLKVCYPYFKSPNIIGSNTWIKGLYDTCSYIGKRAQQVFGVCCTGIQMAAEEIKD-SSENNNKPTQP-PFPIKRQEDNNKPTQPSLPIKKQEDNIEVTHQKNSLEKDYDIMEGEEDQELEIRNTTNTKDLLGVKLSSDHCGQRFFSNNLKIVNGEDASVDEFPFMAALFNKXRHFXGGSIIDSKHILTAAHCVAHMTKIDVRYLRVHVRQYRIKSSNIGTEQEFRVRRVIRHKSFSANTLYNDVAILTLRGSIDFSKSVQPICLASDNTETYEGDIVTVAGWGTLSDGGRQSSILQKVSVGVWSNYDCGASYGSRAPGGIKSHMLCAASKGKDSCSGDSGGPLFYCDTYCTQIGIVSWGIGSK----PDSYDD---------------DHPSGQSSNIYQHSKFPCTSSNGRS------GRCISFKSCYPFHRVRSDVPEWVFGDANVCKNRNPREAQDQRREICCENVYQSILGFYPIYPVH--VIYVPGVDG 568          

HSP 3 Score: 243.817 bits (621), Expect = 3.347e-68
Identity = 119/264 (45.08%), Postives = 173/264 (65.53%), Query Frame = 0

Query:  189 PINPDEFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCT-DRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            PI  DE   +  V+E   +  +  +L +IVNG  A +HE+P++AALF   RQFCGGSL+ ++HILTAAHCV  M+++DV  ++V LG+HN++   +   +  +V  ++RHK F + TL NDVA+L LS+   ++  ++PIC++       +  +  V GWGS+ E G Q  IL++V + +W N+KC+ +Y   +P GIT HMICA    +DSCSGDSGGP+     D+  QIGIVSWGIGC +  FPGVYTRV ++  WI K++
Sbjct:  639 PIVYDE---NGSVSEEINRIDMEHDL-KIVNGWPASKHEWPWIAALFNRGRQFCGGSLISRSHILTAAHCVAQMTRSDVNNLEVRLGEHNIKDALDTQVEVRKVNKIIRHKGFSSQTLWNDVALLFLSDPVSYSDIIKPICIDTSDDPYDKNXTATVVGWGSLRESGPQPXILQEVTLRLWKNEKCQETYNGASPAGITDHMICAGRQGKDSCSGDSGGPMVKAKGDKWVQIGIVSWGIGCGKSHFPGVYTRVGKMYEWITKVI 898          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000012442 (supercontig:LSalAtl2s:LSalAtl2s914:161702:164343:-1 gene:EMLSAG00000012442 transcript:EMLSAT00000012442 description:"maker-LSalAtl2s914-augustus-gene-2.11")

HSP 1 Score: 210.305 bits (534), Expect = 4.525e-59
Identity = 101/247 (40.89%), Postives = 148/247 (59.92%), Query Frame = 0

Query:  679 EHPLRVVNGWPADKNEWPWIAALLR----SGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNG-DRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERAR 920
             +  R+V G PAD NEWPW+AA++R    S  QFCG +LI   H++TAAHCVA      ++   VRLGE+N   EGE+      +  +  H+ + +   YND+A++ L    P + S +I P+CLP     Y  R G V+GWG++   GP  ++LQE+ + +WDN +C + Y  ++   +   M+CAG +G+D+C GDSGGP    N   +WE  G+VSWG  C + D+PGVYTR T+   WIE+ +
Sbjct:  287 RYSTRIVGGRPADPNEWPWLAAMIRPSEVSSGQFCGATLISDRHVITAAHCVAPFKQDQIK---VRLGEYNFNAEGETLDRTYNLESIKMHELYDAVKYYNDIAIMKLDR--PVTFSKSIYPICLPPPIETYEKRRGYVIGWGTIYFGGPTSNILQEVNVAVWDNNKCKENYSALN-RNVTSKMLCAGDRGRDACQGDSGGPLNCLNAFSQWELCGVVSWGARCAEPDYPGVYTRVTEYLDWIEQNK 527          

HSP 2 Score: 188.348 bits (477), Expect = 2.217e-51
Identity = 96/241 (39.83%), Postives = 147/241 (61.00%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFK----NSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQ---IGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AA+ +    +S QFCG +L+   H++TAAHCV    Q+   Q+KV LG++N    GE  ++ + ++++  H+ +     +ND+A++ L     F+ ++ PICL    +  YE R   V GWG+I   G  S IL++V+V VW N+KC+ +Y +     +T+ M+CA    RD+C GDSGGPL +C +   Q    G+VSWG  CA P +PGVYTRVTE + WI++
Sbjct:  291 RIVGGRPADPNEWPWLAAMIRPSEVSSGQFCGATLISDRHVITAAHCVAPFKQD---QIKVRLGEYNFNAEGETLDRTYNLESIKMHELYDAVKYYNDIAIMKLDRPVTFSKSIYPICLPPP-IETYEKRRGYVIGWGTIYFGGPTSNILQEVNVAVWDNNKCKENY-SALNRNVTSKMLCAGDRGRDACQGDSGGPL-NCLNAFSQWELCGVVSWGARCAEPDYPGVYTRVTEYLDWIEQ 525          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000007886 (supercontig:LSalAtl2s:LSalAtl2s464:52646:53754:1 gene:EMLSAG00000007886 transcript:EMLSAT00000007886 description:"maker-LSalAtl2s464-augustus-gene-0.4")

HSP 1 Score: 165.236 bits (417), Expect = 4.752e-46
Identity = 93/235 (39.57%), Postives = 135/235 (57.45%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAAL-FKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            +IV GTEA+ H  PF  +   KN   FCGGS+LD+T ++TA HC +  S NDV   +V +G H+  +  E  EQ   +  +  H+ F +  ++ND+ +L +     F   V+P+ L +   F   G  V V+GWG++  +G+ S +LR V + +    +C  +Y     GG+   MICA+G  +DSC GDSGGPL         +GIVSWGIGCA P FPGVYT+V+  + WI +
Sbjct:   38 KIVGGTEAQPHSIPFQVSFQRKNGFHFCGGSILDETTVITAGHCCKGFSINDV---QVVVGAHDFNSP-EGTEQTQNIVKITYHENFASKGINNDICLLEVEHPFEFNDNVKPVTLPEKE-FTPTGEVV-VSGWGTLRANGNSSPVLRTVTLNMVPYLRCYINY----IGGLDESMICASGKGKDSCQGDSGGPL---VQENTLVGIVSWGIGCAHPWFPGVYTKVSMFIDWIHE 259          

HSP 2 Score: 150.984 bits (380), Expect = 3.723e-41
Identity = 90/240 (37.50%), Postives = 131/240 (54.58%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLR-SGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRR-EGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERAR 920
            ++V G  A  +  P+  +  R +G  FCGGS++D T ++TA HC    S  DVQ++   +G H+    EG   T    + ++  H+ F+S  + ND+ L+ +++  P   +D ++PV LP      TG +  V GWG+LR NG    VL+ +T+ +    RC   Y G    G+ + M+CA  KGKDSC GDSGGP +  N      VGIVSWGIGC    FPGVYT+ +    WI   +
Sbjct:   38 KIVGGTEAQPHSIPFQVSFQRKNGFHFCGGSILDETTVITAGHCCKGFSINDVQVV---VGAHDFNSPEGTEQT--QNIVKITYHENFASKGINNDICLLEVEH--PFEFNDNVKPVTLPEKEFTPTGEV-VVSGWGTLRANGNSSPVLRTVTLNMVPYLRCYINYIG----GLDESMICASGKGKDSCQGDSGGPLVQEN----TLVGIVSWGIGCAHPWFPGVYTKVSMFIDWIHENK 261          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000008660 (supercontig:LSalAtl2s:LSalAtl2s537:130430:177832:-1 gene:EMLSAG00000008660 transcript:EMLSAT00000008660 description:"maker-LSalAtl2s537-augustus-gene-2.26")

HSP 1 Score: 176.022 bits (445), Expect = 2.749e-45
Identity = 92/239 (38.49%), Postives = 138/239 (57.74%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSG--RQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYN-DVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG-KKGK-DSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            R+V G   ++NE+PW   +++ G  + FCGG+L+   H++TA HC      Q    + + LGEHNI+   ++     PV +V  HK FS   + + D+ L+TL+   P      I+P+CLP     + G + T+ GWG L E GPQPD+LQE+ + +  N  C + Y+ ++P      M+CAG KKG+ D+C GDSGGP      +R+   G  S+G GC + DFPGVY+R T ++ WI
Sbjct:  827 RIVGGKEVEENEYPWHIGIVKIGDNKPFCGGALVTDRHVVTAGHCF-----QTFHKVEILLGEHNIQNSNDT-ALRIPVKKVEVHKEFSVLAMNDYDITLLTLER--PIKFRREIQPICLPFKDEDFVGTMCTITGWGRLNETGPQPDILQEVNVPVITNDVCKKSYETLTP-----RMICAGYKKGELDACQGDSGGPLSCPQNNRYVLAGSTSYGFGCARPDFPGVYSRITLLQDWI 1052          

HSP 2 Score: 164.466 bits (415), Expect = 1.083e-41
Identity = 91/241 (37.76%), Postives = 136/241 (56.43%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQ--FCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYN-DVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG-KKGK-DSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            R+V G   ++NE+PW   +++ G+   FCGGSL+   H++TAAHC   +       + V LGEHNI+   ++     PV +V  HK F      + DV L+TL+   P      I+P+CLP     + G + T+ GWG L E  PQPD+LQE+ + +  N  C++ Y+ ++P      M+CAG +KG+ DSC GDS  P      +R+   GI S+G GC +  FPGVY+R T ++ WI+ 
Sbjct:  162 RIVGGKEVEENEYPWQIGIVKIGKNKPFCGGSLVSDRHVVTAAHCFKTLYK-----VEVLLGEHNIQNSNDT-ALRIPVKKVEVHKEFGVLAFNDYDVTLLTLEQ--PIKFRREIQPICLPFKDEDFVGTMCTITGWGRLSETEPQPDILQEVNVPVITNDVCNKAYETITP-----RMICAGYEKGEIDSCQGDS--PLSCPQNNRYVFTGITSFGHGCARPGFPGVYSRITLLQDWIKE 387          

HSP 3 Score: 156.377 bits (394), Expect = 4.046e-39
Identity = 95/255 (37.25%), Postives = 143/255 (56.08%), Query Frame = 0

Query:  205 CGKRSVGSNLVRIVNGTEAKQHEFPFMAALFK--NSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHN-DVAVLTLSEAAVFTPTVQPICL---EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTF--RDSCSGDSGGPLFDCTDRCRQI--GIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            CG ++V S   RIV G E +++E+P+   + K  +++ FCGG+L+   H++TA HC +        ++++ LG+HN++   + A  +  VK V  HK F    +++ D+ +LTL     F   +QPICL   ++D    + G    + GWG + E G Q  IL++V+V V +ND C++SY    P      MICA       D+C GDSGGPL  C    R +  G  S+G GCARP FPGVY+R+T L  WI +
Sbjct:  819 CGTKNVNS---RIVGGKEVEENEYPWHIGIVKIGDNKPFCGGALVTDRHVVTAGHCFQTF-----HKVEILLGEHNIQNSNDTA-LRIPVKKVEVHKEFSVLAMNDYDITLLTLERPIKFRREIQPICLPFKDED----FVGTMCTITGWGRLNETGPQPDILQEVNVPVITNDVCKKSYETLTP-----RMICAGYKKGELDACQGDSGGPL-SCPQNNRYVLAGSTSYGFGCARPDFPGVYSRITLLQDWINE 1054          

HSP 4 Score: 149.058 bits (375), Expect = 8.480e-37
Identity = 99/256 (38.67%), Postives = 145/256 (56.64%), Query Frame = 0

Query:  205 CGKRSVGSNLVRIVNGTEAKQHEFPFMAALFK--NSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHN-DVAVLTLSEAAVFTPTVQPICL---EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICA---AGTFRDSCSGDSGGPLFDCTDRCRQI--GIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            CG ++V S   RIV G E +++E+P+   + K   ++ FCGGSL+   H++TAAHC + + +     ++V LG+HN++   + A  +  VK V  HK F     ++ DV +LTL +   F   +QPICL   ++D    + G    + GWG ++E   Q  IL++V+V V +ND C ++Y       IT  MICA    G   DSC GDS  PL  C    R +  GI S+G GCARP FPGVY+R+T L  WI++
Sbjct:  154 CGTKNVNS---RIVGGKEVEENEYPWQIGIVKIGKNKPFCGGSLVSDRHVVTAAHCFKTLYK-----VEVLLGEHNIQNSNDTA-LRIPVKKVEVHKEFGVLAFNDYDVTLLTLEQPIKFRREIQPICLPFKDED----FVGTMCTITGWGRLSETEPQPDILQEVNVPVITNDVCNKAYET-----ITPRMICAGYEKGEI-DSCQGDS--PL-SCPQNNRYVFTGITSFGHGCARPGFPGVYSRITLLQDWIKE 387          

HSP 5 Score: 108.997 bits (271), Expect = 2.017e-24
Identity = 57/160 (35.62%), Postives = 88/160 (55.00%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQ--FCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYN-DVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDN 839
            R+V G   ++NE+PW   +++ G    FCGG+L+   H++TA HC      Q  + + + LGEHNIR   ++      V +V  HK F+     + D+ L+TL+   P      I+P+CLP     + G + T+ GWG L E GPQPD+LQE+ + +  N
Sbjct:  533 RIVGGKEVEENEYPWHIGIVKIGENLPFCGGALVTDRHVVTAGHCF-----QTFRKVEILLGEHNIRNSHDT-ALRIRVKKVEVHKEFNIIAKNDYDITLLTLER--PIKFRREIQPICLPFKDDDFVGTMCTITGWGRLNETGPQPDILQEVNVPVITN 684          

HSP 6 Score: 101.293 bits (251), Expect = 5.785e-22
Identity = 61/179 (34.08%), Postives = 100/179 (55.87%), Query Frame = 0

Query:  205 CGKRSVGSNLVRIVNGTEAKQHEFPFMAALFK--NSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHN-DVAVLTLSEAAVFTPTVQPICL--EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSY 378
            CG ++V S   RIV G E +++E+P+   + K   +  FCGG+L+   H++TA HC +       R++++ LG+HN+R   + A  + RVK V  HK F     ++ D+ +LTL     F   +QPICL  + D   ++ G    + GWG + E G Q  IL++V+V V +ND  + ++
Sbjct:  525 CGTKNVNS---RIVGGKEVEENEYPWHIGIVKIGENLPFCGGALVTDRHVVTAGHCFQTF-----RKVEILLGEHNIRNSHDTA-LRIRVKKVEVHKEFNIIAKNDYDITLLTLERPIKFRREIQPICLPFKDD---DFVGTMCTITGWGRLNETGPQPDILQEVNVPVITNDDFQSAF 691          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000002149 (supercontig:LSalAtl2s:LSalAtl2s139:22566:31405:1 gene:EMLSAG00000002149 transcript:EMLSAT00000002149 description:"maker-LSalAtl2s139-augustus-gene-0.10")

HSP 1 Score: 159.844 bits (403), Expect = 7.417e-42
Identity = 90/247 (36.44%), Postives = 140/247 (56.68%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSG----RQFCGGSLIDSTHILTAAHCV--AQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTV---VGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKK--GKDSCSGDSGGPFMIGNGDR--WEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            R+V G    + E+PW+AA+   G      +CGG+L+   H++TAAHC   A+      +  T+RLGE ++  + E+ + E  +  V+ H  F ++  Y+DVA+  LK   P   S+ I+P+CLP+GS +    LGT+   +GWG+    G + + L+ + + +W+N  CD  Y    P  I +  +CAG    G+D+C GDSGGP M+ +  R  W  +G+VS+G  C +A +PGVYTR T    WI
Sbjct:  253 RIVGGKATYEGEYPWMAAIYLHGGGRTEFWCGGALVSERHVITAAHCTKDAKKKQFKARQFTIRLGEWDLD-DKETYSREYEIVDVIAHPDFKANGFYSDVAI--LKTIEPVEFSEYIQPICLPTGSMKNDNFLGTLPVALGWGATHYRGKEVNKLRGVALPVWNNKDCDGAY--FQP--ITEVFLCAGYASGGRDACQGDSGGPLMLYSQKRKAWVLIGVVSFGNRCAEAGYPGVYTRLTHFLDWI 492          

HSP 2 Score: 142.895 bits (359), Expect = 3.218e-36
Identity = 86/245 (35.10%), Postives = 132/245 (53.88%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKN----SRQFCGGSLLDKTHILTAAHCVE--HMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFN--YEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICA--AGTFRDSCSGDSGGPLFDCTDRCRQ---IGIVSWGIGCARPVFPGVYTRVTELMPWI 447
            RIV G    + E+P+MAA++ +    +  +CGG+L+ + H++TAAHC +     Q   RQ  + LG+ +L  + E   +++ +  V+ H  FK +  ++DVA+L   E   F+  +QPICL    + N  + G      GWG+    G +   LR V + VW+N  C  +Y       IT   +CA  A   RD+C GDSGGPL   + + +    IG+VS+G  CA   +PGVYTR+T  + WI
Sbjct:  253 RIVGGKATYEGEYPWMAAIYLHGGGRTEFWCGGALVSERHVITAAHCTKDAKKKQFKARQFTIRLGEWDLDDK-ETYSREYEIVDVIAHPDFKANGFYSDVAILKTIEPVEFSEYIQPICLPTGSMKNDNFLGTLPVALGWGATHYRGKEVNKLRGVALPVWNNKDCDGAYFQP----ITEVFLCAGYASGGRDACQGDSGGPLMLYSQKRKAWVLIGVVSFGNRCAEAGYPGVYTRLTHFLDWI 492          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000004828 (supercontig:LSalAtl2s:LSalAtl2s254:397245:398159:-1 gene:EMLSAG00000004828 transcript:EMLSAT00000004828 description:"augustus_masked-LSalAtl2s254-processed-gene-4.1")

HSP 1 Score: 150.599 bits (379), Expect = 6.290e-41
Identity = 92/237 (38.82%), Postives = 133/237 (56.12%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAAL-FKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNL-RTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICL-EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G E + +  PF  +        FCG S++DK  I+TAAHC +    ++V+   V  G+H+L  T G+  EQ+  V  +  H++F +   + DV +L L  +  F   V+PI L E+D  F  +   V V+GWG+I+  G  S +L+ V V+V S++ C  +Y     G I   MICAA   +DSC GDSGGPL         +GIVSWG GCA P +PGVY +V++ + WI +
Sbjct:   41 RIVGGEEVEPNSIPFQISFQTTGGFHFCGASVMDKDTIITAAHCCDSFYPSEVQ---VVAGEHDLFSTSGD--EQKIAVSDITYHEKFASHGTNYDVCLLKLKSSLHFNEKVKPIALPEKDQEFIGD---VVVSGWGTISSSGPPSPVLKAVTVQVVSDEDCSDAY----YGSIDETMICAAAPGKDSCQGDSGGPL---AQDGTLVGIVSWGYGCAAPGYPGVYGKVSKFIDWIAE 262          

HSP 2 Score: 134.806 bits (338), Expect = 1.515e-35
Identity = 83/261 (31.80%), Postives = 132/261 (50.57%), Query Frame = 0

Query:  661 ISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPW-IAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERAR 920
            I Y E+   V++++   G    R+V G   + N  P+ I+     G  FCG S++D   I+TAAHC       +VQ++    GEH++      +  +  V+ +  H+ F+S     DV L+ LK+++    ++ ++P+ LP     + G +  V GWG++  +GP   VL+ +T+++  +  C   Y G     I + M+CA   GKDSC GDSGGP           VGIVSWG GC    +PGVY + +    WI   +
Sbjct:   22 IKYSESFMKVKSMRHRFGG---RIVGGEEVEPNSIPFQISFQTTGGFHFCGASVMDKDTIITAAHCCDSFYPSEVQVVA---GEHDLFST-SGDEQKIAVSDITYHEKFASHGTNYDVCLLKLKSSL--HFNEKVKPIALPEKDQEFIGDV-VVSGWGTISSSGPPSPVLKAVTVQVVSDEDCSDAYYG----SIDETMICAAAPGKDSCQGDSGGPL----AQDGTLVGIVSWGYGCAAPGYPGVYGKVSKFIDWIAEHQ 264          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000012246 (supercontig:LSalAtl2s:LSalAtl2s892:54138:171852:1 gene:EMLSAG00000012246 transcript:EMLSAT00000012246 description:"maker-LSalAtl2s892-augustus-gene-0.10")

HSP 1 Score: 154.836 bits (390), Expect = 1.275e-40
Identity = 86/235 (36.60%), Postives = 124/235 (52.77%), Query Frame = 0

Query:  690 ADKNEWPWIAALLRSGRQF--CGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFET-PVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGT--VVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQG-VSPA-GIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIE 917
            AD  EWPW AA+L   +    CG +L+D + +LTAAHCV            VRLGE+++    E    E   VT +V H GF++ TL +D+AL+ L+   P      I  VC+P        +     V GWG   E      VL+E+ + +W    C+   +    PA  +    +CAG +G+D+C GD GGP +     +W Q+GIVS+GIGCG+ + PGVYT+ ++   WIE
Sbjct:  189 ADMAEWPWHAAILEKPQDLYVCGSTLLDESWVLTAAHCVDDYLNTWYIFSIVRLGEYDVSTTAEPLRHEEFNVTDIVMHPGFNNSTLVHDIALLKLER--PAKRKQNIDVVCMPKSRDFNPSQFARCYVTGWGRRTETSEHSLVLKEIEVPLWSYTNCNGALKAQFGPAYSLPTXAICAGAEGRDACDGDGGGPLVCEKEGKWYQIGIVSFGIGCGRKNIPGVYTKVSEXDSWIE 421          

HSP 2 Score: 140.969 bits (354), Expect = 5.371e-36
Identity = 88/254 (34.65%), Postives = 132/254 (51.97%), Query Frame = 0

Query:  205 CGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQF--CGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGE-AAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFN-YEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQ-APG-GITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            CG++   S      N   A   E+P+ AA+ +  +    CG +LLD++ +LTAAHCV+           V LG++++ T  E    ++F V  +V H  F  STL +D+A+L L   A     +  +C+ +   FN  +     V GWG   E    S +L++++V +WS   C  +   Q  P   + T  ICA    RD+C GD GGPL  +   +  QIGIVS+GIGC R   PGVYT+V+E   WI+ ++
Sbjct:  176 CGRKGFDSG-----NSGGADMAEWPWHAAILEKPQDLYVCGSTLLDESWVLTAAHCVDDYLNTWYIFSIVRLGEYDVSTTAEPLRHEEFNVTDIVMHPGFNNSTLVHDIALLKLERPAKRKQNIDVVCMPKSRDFNPSQFARCYVTGWGRRTETSEHSLVLKEIEVPLWSYTNCNGALKAQFGPAYSLPTXAICAGAEGRDACDGDGGGPLVCEKEGKWYQIGIVSFGIGCGRKNIPGVYTKVSEXDSWIENVI 424          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000010925 (supercontig:LSalAtl2s:LSalAtl2s730:283497:284811:1 gene:EMLSAG00000010925 transcript:EMLSAT00000010925 description:"maker-LSalAtl2s730-augustus-gene-3.19")

HSP 1 Score: 143.665 bits (361), Expect = 6.248e-39
Identity = 91/235 (38.72%), Postives = 128/235 (54.47%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAAL-FKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNL-RTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICL-EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWI 447
            RIV G E + +  PF  +        FCG S++DK  I+TAAHC +     +V+   V  G+H+L  T G+  EQ   V  +  H+ F +   + DV +L L  +  F   V+PI L E+D  F  +   V V+GWG+I+  G  S +L+ V V+V S++ C  +Y     G I   MICAA   + SC GDSGGPL         +GIVSWG GCA P +PGVY +V++ + WI
Sbjct:   13 RIVGGEEVEPNSIPFQVSFQTTGGFHFCGASVMDKDTIITAAHCCDSFFPWEVQ---VVAGEHDLFSTSGD--EQTIDVSDITYHEMFASHGTNYDVCLLKLKSSLHFNEKVKPIALPEKDQEFIGD---VVVSGWGTISSSGPASPVLKAVTVQVVSDEDCSDAY----YGSIDETMICAAAPGKXSCQGDSGGPL---AQDGTLVGIVSWGYGCAAPGYPGVYGKVSKFIDWI 232          

HSP 2 Score: 129.798 bits (325), Expect = 4.526e-34
Identity = 78/236 (33.05%), Postives = 122/236 (51.69%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSG-RQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNI-RREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            R+V G   + N  P+  +   +G   FCG S++D   I+TAAHC       +VQ++    GEH++    G+  T +  V+ +  H+ F+S     DV L+ LK+++    ++ ++P+ LP     + G +  V GWG++  +GP   VL+ +T+++  +  C   Y G     I + M+CA   GK SC GDSGGP           VGIVSWG GC    +PGVY + +    WI
Sbjct:   13 RIVGGEEVEPNSIPFQVSFQTTGGFHFCGASVMDKDTIITAAHCCDSFFPWEVQVVA---GEHDLFSTSGDEQTID--VSDITYHEMFASHGTNYDVCLLKLKSSL--HFNEKVKPIALPEKDQEFIGDV-VVSGWGTISSSGPASPVLKAVTVQVVSDEDCSDAYYG----SIDETMICAAAPGKXSCQGDSGGPL----AQDGTLVGIVSWGYGCAAPGYPGVYGKVSKFIDWI 232          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000004364 (supercontig:LSalAtl2s:LSalAtl2s229:72652:77339:1 gene:EMLSAG00000004364 transcript:EMLSAT00000004364 description:"augustus_masked-LSalAtl2s229-processed-gene-1.2")

HSP 1 Score: 145.591 bits (366), Expect = 6.561e-38
Identity = 87/267 (32.58%), Postives = 130/267 (48.69%), Query Frame = 0

Query:  668 EDVEAVQRIEGEHPLRVVNGWPADKNEWPW-----IAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESN-TFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVS-PAGIKDHMMCAGKK--GKDSCSGDSGGPFMIGNGDR--WEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            ED+ + +        R++ G PA    WPW     I A   S    CG  LI  +  +TAAHCV     +++ +   R+G++N   E E     +   ++++ H  ++     ND+AL+  K  VP      IR +CLP       G+ G V GWG + E GP+P +L E+ + I+ + RC  ++        + D  +CAG++  GKD+C GDSGGP +  +  R  W   GI SWG GCG+   PGVYTR      W+      F
Sbjct:  128 EDLTSRRLFNSRPQSRLIGGEPALFGAWPWQISLRIIASDESSNHRCGAVLISESWAITAAHCVHYYEMEEILL---RMGDYNRVEEDEPLPHMDRRPSKIIEHPKYNPRNFENDIALMHFKKPVP--FLPHIRSICLPEQGEDVVGKTGYVTGWGEIYEEGPRPRILHEVDIPIFGHKRCTDLFTKADLDELVTDVFICAGEEEGGKDACEGDSGGPLVFKSKKRGQWVLAGITSWGRGCGEKYQPGVYTRVPIYVEWVLETIKNF 389          

HSP 2 Score: 118.627 bits (296), Expect = 7.441e-29
Identity = 87/312 (27.88%), Postives = 150/312 (48.08%), Query Frame = 0

Query:  163 IFGICCVEKNIKADLLAE--IEALRTEE--PINPD-----EFPLDAQVNENCGKRSVGSNL--VRIVNGTEAKQHEFPFMAALF-----KNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAA-EQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTF---RDSCSGDSGGPL-FDCTDRCRQI--GIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            +FG CC  K  + +L+ E  +EA+  EE   + P       F       E+   R + ++    R++ G  A    +P+  +L      ++S   CG  L+ ++  +TAAHCV +    ++ ++ + +GD+N     E       R   ++ H ++      ND+A++   +   F P ++ ICL + G  +  G++  V GWG I E+G +  IL +VD+ ++ + +C   +       + T +   AG     +D+C GDSGGPL F    R + +  GI SWG GC     PGVYTRV   + W+ + +
Sbjct:   79 LFGSCCKLKFNQQNLIEENEVEAILNEEDSSLLPTATCGMRFNDFTTPEEDLTSRRLFNSRPQSRLIGGEPALFGAWPWQISLRIIASDESSNHRCGAVLISESWAITAAHCVHYY---EMEEILLRMGDYNRVEEDEPLPHMDRRPSKIIEHPKYNPRNFENDIALMHFKKPVPFLPHIRSICLPEQGE-DVVGKTGYVTGWGEIYEEGPRPRILHEVDIPIFGHKRCTDLFTKADLDELVTDVFICAGEEEGGKDACEGDSGGPLVFKSKKRGQWVLAGITSWGRGCGEKYQPGVYTRVPIYVEWVLETI 386          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000002836 (supercontig:LSalAtl2s:LSalAtl2s1638:13958:14826:-1 gene:EMLSAG00000002836 transcript:EMLSAT00000002836 description:"maker-LSalAtl2s1638-augustus-gene-0.2")

HSP 1 Score: 140.584 bits (353), Expect = 1.068e-37
Identity = 85/236 (36.02%), Postives = 128/236 (54.24%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAAL-FKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICL-EQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G E + +  PF  ++       FCGGS++DK  I+TA HC  ++   D+    V  G+H+  +     EQ+  V  + RH +F T   + D+ +L L  +      V+P+ L E+D  F  +   V V+GWG++   GS S +LR V +++ S++ C  +Y     G     MICA    +DSC GDSGGPL   T     +GIVSWG GCA P +PG+Y +V++ + WI +
Sbjct:   26 RIVGGDEVEPNSIPFQISIEISGGYHFCGGSVMDKDTIITAGHCCTNLKPEDIL---VVAGEHDFLSM-SGDEQKIAVSEMXRHDKFGTYGPNYDICLLKLKSSLALNEKVKPVALPEKDQEFTGD---VVVSGWGTVEFGGSSSDVLRSVTLQLVSDEYCSDAY----DGHTDESMICAGSPGKDSCQGDSGGPL---TQDGTLVGIVSWGNGCAFPGYPGIYGKVSKFIDWIAE 247          

HSP 2 Score: 125.561 bits (314), Expect = 1.597e-32
Identity = 78/244 (31.97%), Postives = 124/244 (50.82%), Query Frame = 0

Query:  679 EHPLRVVNGWPADKNEWPW-IAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHN-IRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERAR 920
            +H  R+V G   + N  P+ I+  +  G  FCGGS++D   I+TA HC   +  +D+ ++    GEH+ +   G+    +  V+ + RH  F   T   +  +  LK     ++++ ++PV LP     +TG +  V GWG++   G   DVL+ +T+++  +  C   Y G +     + M+CAG  GKDSC GDSGGP           VGIVSWG GC    +PG+Y + +    WI   +
Sbjct:   22 KHSGRIVGGDEVEPNSIPFQISIEISGGYHFCGGSVMDKDTIITAGHCCTNLKPEDILVVA---GEHDFLSMSGDEQ--KIAVSEMXRHDKFG--TYGPNYDICLLKLKSSLALNEKVKPVALPEKDQEFTGDV-VVSGWGTVEFGGSSSDVLRSVTLQLVSDEYCSDAYDGHT----DESMICAGSPGKDSCQGDSGGPLT----QDGTLVGIVSWGNGCAFPGYPGIYGKVSKFIDWIAEQK 249          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|2499864|sp|Q90629.1|TRY3_CHICK (RecName: Full=Trypsin II-P29; Flags: Precursor)

HSP 1 Score: 148.288 bits (373), Expect = 6.500e-39
Identity = 94/238 (39.50%), Postives = 133/238 (55.88%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQ-PDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIE 917
            ++V G+   ++  P+  +L  SG  FCGGSLI+S  +L+AAHC           + VRLGE+NI  + +S    +  + ++RH  +SS TL ND+ LI L +AV    S  I+P+ LPS   +  G    + GWG+   NG   P++LQ L   I  +  C + Y    P  I  +M+C G  + GKDSC GDSGGP ++ NG   E  GIVSWGIGC    +PGVYT+  +   WI+
Sbjct:   25 KIVGGYTCPEHSVPYQVSL-NSGYHFCGGSLINSQWVLSAAHCYKSR-------IQVRLGEYNIDVQEDSEVVRSS-SVIIRHPKYSSITLNNDIMLIKLASAV--EYSADIQPIALPSSCAK-AGTECLISGWGNTLSNGYNYPELLQCLNAPILSDQECQEAY----PGDITSNMICVGFLEGGKDSCQGDSGGP-VVCNG---ELQGIVSWGIGCALKGYPGVYTKVCNYVDWIQ 242          

HSP 2 Score: 138.658 bits (348), Expect = 1.756e-35
Identity = 81/244 (33.20%), Postives = 138/244 (56.56%), Query Frame = 0

Query:  211 GSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDG-SQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAA--GTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            G++  +IV G    +H  P+  +L  +   FCGGSL++   +L+AAHC +        +++V LG++N+  + E +E       ++RH ++ + TL+ND+ ++ L+ A  ++  +QPI L         G    ++GWG+   +G +   +L+ ++  + S+ +C+ +Y    PG IT++MIC       +DSC GDSGGP+  C    +  GIVSWGIGCA   +PGVYT+V   + WIQ+ +
Sbjct:   20 GADDDKIVGGYTCPEHSVPYQVSL-NSGYHFCGGSLINSQWVLSAAHCYK-------SRIQVRLGEYNIDVQ-EDSEVVRSSSVIIRHPKYSSITLNNDIMLIKLASAVEYSADIQPIALPSS--CAKAGTECLISGWGNTLSNGYNYPELLQCLNAPILSDQECQEAY----PGDITSNMICVGFLEGGKDSCQGDSGGPVV-CNGELQ--GIVSWGIGCALKGYPGVYTKVCNYVDWIQETI 245          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|209572782|sp|Q9DBI0.4|TMPS6_MOUSE (RecName: Full=Transmembrane protease serine 6; AltName: Full=Matriptase-2)

HSP 1 Score: 159.458 bits (402), Expect = 6.538e-39
Identity = 98/244 (40.16%), Postives = 138/244 (56.56%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGE--HNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYT-GRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQ-GVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGN-GDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERA 919
            R+V G  + + EWPW A+L   GR  CGG+LI    ++TAAHC  + S    ++ TV LG+   N R  GE +     V+R+  H     D+   DVAL+ L +  P   S T+RPVCLP+ S  +  G+   + GWG+ RE GP  + LQ++ +++     C + Y+  VSP      M+CAG  K  KD+C GDSGGP +      RW   G+VSWG+GCG+ +F GVYTR T V  WI++ 
Sbjct:  576 RIVGGTVSSEGEWPWQASLQIRGRHICGGALIADRWVITAAHCFQEDSMASPKLWTVFLGKMRQNSRWPGEVS---FKVSRLFLHPYHEEDSHDYDVALLQLDH--PVVYSATVRPVCLPARSHFFEPGQHCWITGWGAQREGGPVSNTLQKVDVQLVPQDLCSEAYRYQVSP-----RMLCAGYRKGKKDACQGDSGGPLVCREPSGRWFLAGLVSWGLGCGRPNFFGVYTRVTRVINWIQQV 809          

HSP 2 Score: 158.688 bits (400), Expect = 1.271e-38
Identity = 94/253 (37.15%), Postives = 139/253 (54.94%), Query Frame = 0

Query:  204 NCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGT--FRDSCSGDSGGPLF--DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILT 452
            +CG + + S   RIV GT + + E+P+ A+L    R  CGG+L+    ++TAAHC +  S    +   V+LG     +R    E  F+V  +  H   +  +   DVA+L L    V++ TV+P+CL     F   G+   + GWG+  E G  S  L+KVDV++   D C  +Y  Q    ++  M+CA      +D+C GDSGGPL   + + R    G+VSWG+GC RP F GVYTRVT ++ WIQ++LT
Sbjct:  567 DCGLQGLSS---RIVGGTVSSEGEWPWQASLQIRGRHICGGALIADRWVITAAHCFQEDSMASPKLWTVFLGKMRQNSRW-PGEVSFKVSRLFLHPYHEEDSHDYDVALLQLDHPVVYSATVRPVCLPARSHFFEPGQHCWITGWGAQREGGPVSNTLQKVDVQLVPQDLCSEAYRYQ----VSPRMLCAGYRKGKKDACQGDSGGPLVCREPSGRWFLAGLVSWGLGCGRPNFFGVYTRVTRVINWIQQVLT 811          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|209572718|sp|Q8IU80.3|TMPS6_HUMAN (RecName: Full=Transmembrane protease serine 6; AltName: Full=Matriptase-2)

HSP 1 Score: 159.073 bits (401), Expect = 9.405e-39
Identity = 94/243 (38.68%), Postives = 137/243 (56.38%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGE--HNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYT-GRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMI-GNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERA 919
            R+V G  + + EWPW A+L   GR  CGG+LI    ++TAAHC  + S     + TV LG+   N R  GE +     V+R++ H     D+   DVAL+ L + V  S +  +RPVCLP+ S  +  G    + GWG+LRE GP  + LQ++ +++     C ++Y+      +   M+CAG  K  KD+C GDSGGP +      RW   G+VSWG+GCG+ ++ GVYTR T V  WI++ 
Sbjct:  576 RIVGGAVSSEGEWPWQASLQVRGRHICGGALIADRWVITAAHCFQEDSMASTVLWTVFLGKVWQNSRWPGEVS---FKVSRLLLHPYHEEDSHDYDVALLQLDHPVVRSAA--VRPVCLPARSHFFEPGLHCWITGWGALREGGPISNALQKVDVQLIPQDLCSEVYR----YQVTPRMLCAGYRKGKKDACQGDSGGPLVCKALSGRWFLAGLVSWGLGCGRPNYFGVYTRITGVISWIQQV 809          

HSP 2 Score: 148.288 bits (373), Expect = 2.397e-35
Identity = 89/243 (36.63%), Postives = 130/243 (53.50%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGD--HNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGT--FRDSCSGDSGGPLF--DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILT 452
            RIV G  + + E+P+ A+L    R  CGG+L+    ++TAAHC +  S        V+LG    N R  GE +   F+V  ++ H   +  +   DVA+L L    V +  V+P+CL     F   G    + GWG++ E G  S  L+KVDV++   D C   Y  Q    +T  M+CA      +D+C GDSGGPL     + R    G+VSWG+GC RP + GVYTR+T ++ WIQ+++T
Sbjct:  576 RIVGGAVSSEGEWPWQASLQVRGRHICGGALIADRWVITAAHCFQEDSMASTVLWTVFLGKVWQNSRWPGEVS---FKVSRLLLHPYHEEDSHDYDVALLQLDHPVVRSAAVRPVCLPARSHFFEPGLHCWITGWGALREGGPISNALQKVDVQLIPQDLCSEVYRYQ----VTPRMLCAGYRKGKKDACQGDSGGPLVCKALSGRWFLAGLVSWGLGCGRPNYFGVYTRITGVISWIQQVVT 811          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|136424|sp|P00765.1|TRYP_ASTAS (RecName: Full=Trypsin-1; AltName: Full=Trypsin I)

HSP 1 Score: 147.132 bits (370), Expect = 1.357e-38
Identity = 89/239 (37.24%), Postives = 125/239 (52.30%), Query Frame = 0

Query:  217 IVNGTEAKQHEFP----FMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAA--GTFRDSCSGDSGGPLFDC-TDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQ 448
            IV GT+A   EFP    F       S  FCG S+ ++ + +TA HCV      +   +++  G+ ++    E +EQ   V  ++ H+ F    L ND+++L LS +  F   V PI L   G  +    +V V GWG+ +E G+   +L+KV V + S+ +CR  YG      I   MICA      +DSC GDSGGPL    T      GIVSWG GCARP +PGVYT V+  + WI+
Sbjct:    1 IVGGTDAVLGEFPYQLSFQETFLGFSFHFCGASIYNENYAITAGHCVYGDDYENPSGLQIVAGELDMSVN-EGSEQTITVSKIILHENFDYDLLDNDISLLKLSGSLTFNNNVAPIALPAQG--HTATGNVIVTGWGTTSEGGNTPDVLQKVTVPLVSDAECRDDYGADE---IFDSMICAGVPEGGKDSCQGDSGGPLAASDTGSTYLAGIVSWGYGCARPGYPGVYTEVSYHVDWIK 233          

HSP 2 Score: 134.42 bits (337), Expect = 3.091e-34
Identity = 82/241 (34.02%), Postives = 125/241 (51.87%), Query Frame = 0

Query:  684 VVNGWPADKNEWPWIAAL----LRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIR-REGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIE 917
            +V G  A   E+P+  +     L     FCG S+ +  + +TA HCV     ++   L +  GE ++   EG   T    V++++ H+ F  D L ND++L+ L  ++  + ++ + P+ LP+     TG +  V GWG+  E G  PDVLQ++T+ +  +A C   Y       I D M+CAG  + GKDSC GDSGGP    +       GIVSWG GC +  +PGVYT  +    WI+
Sbjct:    1 IVGGTDAVLGEFPYQLSFQETFLGFSFHFCGASIYNENYAITAGHCVYGDDYENPSGLQIVAGELDMSVNEGSEQTI--TVSKIILHENFDYDLLDNDISLLKLSGSL--TFNNNVAPIALPAQGHTATGNV-IVTGWGTTSEGGNTPDVLQKVTVPLVSDAECRDDY---GADEIFDSMICAGVPEGGKDSCQGDSGGPLAASDTGSTYLAGIVSWGYGCARPGYPGVYTEVSYHVDWIK 233          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|1717790|sp|P50342.1|TRYT_MERUN (RecName: Full=Mast cell tryptase; Flags: Precursor)

HSP 1 Score: 148.288 bits (373), Expect = 1.443e-38
Identity = 92/246 (37.40%), Postives = 130/246 (52.85%), Query Frame = 0

Query:  684 VVNGWPADKNEWPWIAALLRS---GRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRY-TGRLGTVVGWGSLRENG--PQPDVLQELTMEIWDNARCDQ-----IYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            +V G  A  N+WPW  +L  +    R FCGGSLI    +LTAAHCV    A D   + V+L +  +            V+R++ H  F +     D+AL+ LKN  P ++S  + PV LP  S  + +G L  V GWG++  +   P P  L+E+ + + +N  CD      +Y G +   ++D M+CAG +G DSC GDSGGP +      W Q G+VSWG GC   + PG+YTR T    WI R
Sbjct:   26 IVGGQEAPGNKWPWQVSLRANETYWRHFCGGSLIHPQWVLTAAHCVGPTIA-DPNKVRVQLRKQYLYYHDHL----LAVSRIITHPTFYATQNGADIALLELKN--PVNISSHVHPVSLPPASETFPSGTLCWVTGWGNIDNDVSLPPPFPLKEVQVPVVENQLCDLKYHKGVYTGDNIHIVRDDMLCAGNEGHDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCALPNRPGIYTRVTYYLDWIHR 264          

HSP 2 Score: 122.094 bits (305), Expect = 1.649e-29
Identity = 86/252 (34.13%), Postives = 114/252 (45.24%), Query Frame = 0

Query:  217 IVNGTEAKQHEFPFMAALFKNS---RQFCGGSLLDKTHILTAAHCV------EHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAI--LRKVDVEVWSNDKCRRSYGNQAPGGITTH-----MICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            IV G EA  +++P+  +L  N    R FCGGSL+    +LTAAHCV       +  +  +R+  +Y  DH L            V  ++ H  F  +    D+A+L L      +  V P+ L         G    V GWG+I  D S      L++V V V  N  C   Y      G   H     M+CA     DSC GDSGGPL         Q G+VSWG GCA P  PG+YTRVT  + WI + +
Sbjct:   26 IVGGQEAPGNKWPWQVSLRANETYWRHFCGGSLIHPQWVLTAAHCVGPTIADPNKVRVQLRKQYLYYHDHLLA-----------VSRIITHPTFYATQNGADIALLELKNPVNISSHVHPVSLPPASETFPSGTLCWVTGWGNIDNDVSLPPPFPLKEVQVPVVENQLCDLKYHKGVYTGDNIHIVRDDMLCAGNEGHDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCALPNRPGIYTRVTYYLDWIHRYV 266          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|400234|sp|Q02844.1|TRYB1_MOUSE (RecName: Full=Tryptase; AltName: Full=Mast cell protease 7; Short=mMCP-7; AltName: Full=Tryptase alpha/beta-1; Flags: Precursor)

HSP 1 Score: 146.747 bits (369), Expect = 4.584e-38
Identity = 94/250 (37.60%), Postives = 130/250 (52.00%), Query Frame = 0

Query:  684 VVNGWPADKNEWPWIAALLRSG---RQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRY-TGRLGTVVGWGSLRE--NGPQPDVLQELTMEIWDNARCDQIYQ-----GVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNK 922
            +V G  A  N+WPW  +L  +      FCGGSLI    +LTAAHCV    A D   + V+L +  +       T    V++++ H  F       D+AL+ L N  P ++SD + PV LP  S  + +G L  V GWG++    N P P  L+E+ + I +N  CD  Y      G +   ++D M+CAG +G DSC GDSGGP +    D W Q G+VSWG GC + + PG+YTR T    WI     K
Sbjct:   29 IVGGQEAHGNKWPWQVSLRANDTYWMHFCGGSLIHPQWVLTAAHCVGPDVA-DPNKVRVQLRKQYLYYHDHLMT----VSQIITHPDFYIVQDGADIALLKLTN--PVNISDYVHPVPLPPASETFPSGTLCWVTGWGNIDNGVNLPPPFPLKEVQVPIIENHLCDLKYHKGLITGDNVHIVRDDMLCAGNEGHDSCQGDSGGPLVCKVEDTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPK 271          

HSP 2 Score: 116.316 bits (290), Expect = 1.551e-27
Identity = 82/252 (32.54%), Postives = 113/252 (44.84%), Query Frame = 0

Query:  217 IVNGTEAKQHEFPFMAALFKNS---RQFCGGSLLDKTHILTAAHCV------EHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAE--DGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTH-----MICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            IV G EA  +++P+  +L  N      FCGGSL+    +LTAAHCV       +  +  +R+  +Y  DH +            V  ++ H  F       D+A+L L+     +  V P+ L         G    V GWG+I    +      L++V V +  N  C   Y      G   H     M+CA     DSC GDSGGPL     D   Q G+VSWG GCA+P  PG+YTRVT  + WI   +
Sbjct:   29 IVGGQEAHGNKWPWQVSLRANDTYWMHFCGGSLIHPQWVLTAAHCVGPDVADPNKVRVQLRKQYLYYHDHLMT-----------VSQIITHPDFYIVQDGADIALLKLTNPVNISDYVHPVPLPPASETFPSGTLCWVTGWGNIDNGVNLPPPFPLKEVQVPIIENHLCDLKYHKGLITGDNVHIVRDDMLCAGNEGHDSCQGDSGGPLVCKVEDTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYV 269          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|2499862|sp|Q90627.1|TRY1_CHICK (RecName: Full=Trypsin I-P1; Flags: Precursor)

HSP 1 Score: 145.976 bits (367), Expect = 5.060e-38
Identity = 91/246 (36.99%), Postives = 137/246 (55.69%), Query Frame = 0

Query:  676 IEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIR-REGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGP-QPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIE 917
            I  E   ++V G+   ++  P+  +L  SG  FCGGSLI S  +L+AAHC           + V+LGE+N+  ++G   T  +  ++V+RH G++++TL ND+ LI L  A   +++  +  V LP+ S    G    + GWG+   +G   PDVLQ L   +  +++C   Y    P  I  +M+C G    GKDSC GDSGGP ++ NG   +  GIVSWGIGC +  +PGVYT+  +   WI+
Sbjct:   18 ISDEDDDKIVGGYSCARSAAPYQVSL-NSGYHFCGGSLISSQWVLSAAHCYKSS-------IQVKLGEYNLAAQDGSEQTISS--SKVIRHSGYNANTLNNDIMLIKLSKA--ATLNSYVNTVPLPT-SCVTAGTTCLISGWGNTLSSGSLYPDVLQCLNAPVLSSSQCSSAY----PGRITSNMICIGYLNGGKDSCQGDSGGP-VVCNG---QLQGIVSWGIGCAQKGYPGVYTKVCNYVSWIK 242          

HSP 2 Score: 138.658 bits (348), Expect = 1.626e-35
Identity = 82/240 (34.17%), Postives = 132/240 (55.00%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQS-AILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAA--GTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILT 452
            +IV G    +   P+  +L  +   FCGGSL+    +L+AAHC +         ++V LG++NL  + + +EQ      V+RH  +  +TL+ND+ ++ LS+AA     V  + L    +    G +  ++GWG+    GS    +L+ ++  V S+ +C  +Y    PG IT++MIC       +DSC GDSGGP+  C  + +  GIVSWGIGCA+  +PGVYT+V   + WI+  ++
Sbjct:   25 KIVGGYSCARSAAPYQVSL-NSGYHFCGGSLISSQWVLSAAHCYK-------SSIQVKLGEYNLAAQ-DGSEQTISSSKVIRHSGYNANTLNNDIMLIKLSKAATLNSYVNTVPLPTSCVT--AGTTCLISGWGNTLSSGSLYPDVLQCLNAPVLSSSQCSSAY----PGRITSNMICIGYLNGGKDSCQGDSGGPVV-CNGQLQ--GIVSWGIGCAQKGYPGVYTKVCNYVSWIKTTMS 246          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|118573093|sp|Q7RTY7.2|OVCH1_HUMAN (RecName: Full=Ovochymase-1; Flags: Precursor)

HSP 1 Score: 157.147 bits (396), Expect = 6.576e-38
Identity = 100/279 (35.84%), Postives = 141/279 (50.54%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMIC---AAGTFRDSCSGDSGGPLFDCTDRCRQ-------IGIVSWGIGCARPVFPGVYTRVTELMPWIQ-KILTPLDIKAG--ADTLVNSI-GPGPKSTPSHWAG 480
            RI  G EA  H +P+   L       CGG++++   ILTAAHCV+   +N+     +  GDH+ R   E+ EQ  R K ++ H+ F T +  +D+A++ LS    +   V+P+CL       +      V GWGSI+ DG  ++ L+++ V V   + C  +Y +  PGGIT  MIC   AA   +D C GDSGGPL      CR         GIVSWG GC +P  PGV+ RV   + WIQ KI  P  ++      TL   +  P P    + W G
Sbjct:  574 RIAGGEEACPHCWPWQVGLRFLGDYQCGGAIINPVWILTAAHCVQ--LKNNPLSWTIIAGDHD-RNLKESTEQVRRAKHIIVHEDFNTLSYDSDIALIQLSSPLEYNSVVRPVCLPHSAEPLFSSEICAVTGWGSISADGGLASRLQQIQVHVLEREVCEHTYYSAHPGGITEKMICAGFAASGEKDFCQGDSGGPLV-----CRHENGPFVLYGIVSWGAGCVQPWKPGVFARVMIFLDWIQSKINGPASLQTNNKCKTLKQQLPPPTPSPDSASWPG 844          

HSP 2 Score: 133.265 bits (334), Expect = 2.290e-30
Identity = 83/244 (34.02%), Postives = 124/244 (50.82%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLP-SGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKG---KDSCSGDSGGPFMIGNGD-RWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARN 921
            R+  G  A  + WPW   L   G   CGG++I+   ILTAAHCV Q+    +   T+  G+H+ R   ES         ++ H+ F++ +  +D+ALI L +  P   +  +RPVCLP S    ++  +  V GWGS+  +G     LQ++ + + +   C+  Y    P GI + M+CAG      KD C GDSGGP +  + +  +   GIVSWG GC +   PGV+ R      WI+   N
Sbjct:  574 RIAGGEEACPHCWPWQVGLRFLGDYQCGGAIINPVWILTAAHCV-QLKNNPLSW-TIIAGDHD-RNLKESTEQVRRAKHIIVHEDFNTLSYDSDIALIQLSS--PLEYNSVVRPVCLPHSAEPLFSSEICAVTGWGSISADGGLASRLQQIQVHVLEREVCEHTYYSAHPGGITEKMICAGFAASGEKDFCQGDSGGPLVCRHENGPFVLYGIVSWGAGCVQPWKPGVFARVMIFLDWIQSKIN 812          

HSP 3 Score: 124.02 bits (310), Expect = 1.568e-27
Identity = 70/236 (29.66%), Postives = 124/236 (52.54%), Query Frame = 0

Query:  696 PWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSS-DTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYT-GRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGN-GDRWEQVGIVSWGIGCGKADFP----------GVYTRTTDVKGWI 916
            PW  +L      FCGGSLI    ++TAAHC+  +S + ++ +TV  GE+++ ++ +      PV++++ H  ++S + +  D+AL+ LK+ V     + ++P+CLP    +   G L    GWG + +     +VLQE+ + I D+  C+ + + ++   +   M+CAG    G D+C GDSGGP +    G  W   GI SW  GC     P          G++++ +++  +I
Sbjct:   59 PWQVSLKSDEHHFCGGSLIQEDRVVTAAHCLDSLSEKQLKNITVTSGEYSLFQKDKQEQ-NIPVSKIITHPEYNSREYMSPDIALLYLKHKV--KFGNAVQPICLPDSDDKVEPGILCLSSGWGKISKTSEYSNVLQEMELPIMDDRACNTVLKSMNLPPLGRTMLCAGFPDWGMDACQGDSGGPLVCRRGGGIWILAGITSWVAGCAGGSVPVRNNHVKASLGIFSKVSELMDFI 291          

HSP 4 Score: 111.309 bits (277), Expect = 1.392e-23
Identity = 79/277 (28.52%), Postives = 132/277 (47.65%), Query Frame = 0

Query:  201 VNENCGKRSVGSNLV-RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKT-STLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAA--GTFRDSCSGDSGGPLFDCTDRCRQ-------IGIVSWGIGCARPVFP----------GVYTRVTELMPWI-QKILTPLD 455
            VN    + +VGS    RI +   +     P+  +L  +   FCGGSL+ +  ++TAAHC++ +S+  ++ + V  G+++L  + +  EQ   V  ++ H  + +   +  D+A+L L     F   VQPICL         G     +GWG I++    S +L+++++ +  +  C     +     +   M+CA       D+C GDSGGPL      CR+        GI SW  GCA    P          G++++V+ELM +I Q + T LD
Sbjct:   30 VNMKSKEPAVGSRFFSRISSWRNSTVTGHPWQVSLKSDEHHFCGGSLIQEDRVVTAAHCLDSLSEKQLKNITVTSGEYSLFQK-DKQEQNIPVSKIITHPEYNSREYMSPDIALLYLKHKVKFGNAVQPICLPDSDDKVEPGILCLSSGWGKISKTSEYSNVLQEMELPIMDDRACNTVLKSMNLPPLGRTMLCAGFPDWGMDACQGDSGGPLV-----CRRGGGIWILAGITSWVAGCAGGSVPVRNNHVKASLGIFSKVSELMDFITQNLFTGLD 300          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|464949|sp|P35033.1|TRY3_SALSA (RecName: Full=Trypsin-3; AltName: Full=Trypsin III; Flags: Precursor)

HSP 1 Score: 144.436 bits (363), Expect = 1.197e-37
Identity = 93/238 (39.08%), Postives = 131/238 (55.04%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLR-ENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIE 917
            ++V G+   KN   + A+L +SG  FCGGSLI ST +++AAHC           + VRLGEHNI     +  F   V +V+ H  ++S  L ND+ LI L    P S++  +  V LPS S   +G    V GWG+L   +   PD L+ L + I  ++ C+  Y    P  I  +M CAG  + GKDSC GDSGGP ++ NG   +  G+VSWG GC + + PGVYT+  + + WI 
Sbjct:   15 KIVGGYECRKNSASYQASL-QSGYHFCGGSLISSTWVVSAAHCYKSR-------IQVRLGEHNIAVNEGTEQFIDSV-KVIMHPSYNSRNLDNDIMLIKLSK--PASLNSYVSTVALPS-SCASSGTRCLVSGWGNLSGSSSNYPDTLRCLDLPILSSSSCNSAY----PGQITSNMFCAGFMEGGKDSCQGDSGGP-VVCNG---QLQGVVSWGYGCAQRNKPGVYTKVCNYRSWIS 232          

HSP 2 Score: 128.642 bits (322), Expect = 3.592e-32
Identity = 80/240 (33.33%), Postives = 126/240 (52.50%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQS-AILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTF--RDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILT 452
            +IV G E +++   + A+L ++   FCGGSL+  T +++AAHC +        +++V LG+HN+    E  EQ      V+ H  + +  L ND+ ++ LS+ A     V  + L         G    V+GWG+++   S     LR +D+ + S+  C  +Y    PG IT++M CA      +DSC GDSGGP+  C  + +  G+VSWG GCA+   PGVYT+V     WI   ++
Sbjct:   15 KIVGGYECRKNSASYQASL-QSGYHFCGGSLISSTWVVSAAHCYK-------SRIQVRLGEHNIAVN-EGTEQFIDSVKVIMHPSYNSRNLDNDIMLIKLSKPASLNSYVSTVALPSSCAS--SGTRCLVSGWGNLSGSSSNYPDTLRCLDLPILSSSSCNSAY----PGQITSNMFCAGFMEGGKDSCQGDSGGPVV-CNGQLQ--GVVSWGYGCAQRNKPGVYTKVCNYRSWISSTMS 236          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|2507246|sp|P32038.2|CFAD_RAT (RecName: Full=Complement factor D; AltName: Full=Adipsin; AltName: Full=C3 convertase activator; AltName: Full=Endogenous vascular elastase; AltName: Full=Properdin factor D; Flags: Precursor)

HSP 1 Score: 145.206 bits (365), Expect = 1.364e-37
Identity = 84/239 (35.15%), Postives = 137/239 (57.32%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCR-RSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIG-CARPVFPGVYTRVTELMPWIQKILT 452
            RI+ G EA  H  P+MA++  N    CGG+L+D+  +L+AAHC++ +++++V Q  V LG H+L +  E  +  + V++VV H   +  ++ +D+ +  LS  A   P V+P+ L+++      G    VAGWG +   G +  +L+++ V +   + C  R+Y +   G IT +M+CA    RD+C GDSGGPL  C D      +V+WG   C     PGV+TRV   +PWI+ +L+
Sbjct:   25 RILGGQEAMAHARPYMASVQVNGTHVCGGTLVDEQWVLSAAHCMDGVTKDEVVQ--VLLGAHSL-SSPEPYKHLYDVQSVVLHPGSRPDSVEDDLMLFKLSHNASLGPHVRPLPLQREDREVKPGTLCDVAGWGVVTHAGRRPDVLQQLTVSIMDRNTCNLRTYHD---GAITKNMMCAESNRRDTCRGDSGGPLV-CGDAVE--AVVTWGSRVCGNRRKPGVFTRVATYVPWIENVLS 254          

HSP 2 Score: 119.398 bits (298), Expect = 9.424e-29
Identity = 79/237 (33.33%), Postives = 120/237 (50.63%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYT-GRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIG-CGKADFPGVYTRTTDVKGWIE 917
            R++ G  A  +  P++A++  +G   CGG+L+D   +L+AAHC+  ++  +V  + V LG H++    E       V  VV H G   D++ +D+ L  L +    S+   +RP+ L         G L  V GWG +   G +PDVLQ+LT+ I D   C+   +      I  +MMCA    +D+C GDSGGP + G+        +V+WG   CG    PGV+TR      WIE
Sbjct:   25 RILGGQEAMAHARPYMASVQVNGTHVCGGTLVDEQWVLSAAHCMDGVTKDEV--VQVLLGAHSLSSP-EPYKHLYDVQSVVLHPGSRPDSVEDDLMLFKLSHNA--SLGPHVRPLPLQREDREVKPGTLCDVAGWGVVTHAGRRPDVLQQLTVSIMDRNTCN--LRTYHDGAITKNMMCAESNRRDTCRGDSGGPLVCGDAVE----AVVTWGSRVCGNRRKPGVFTRVATYVPWIE 250          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1022771057|gb|KZS15399.1| (Serine protease 42 [Daphnia magna])

HSP 1 Score: 408.297 bits (1048), Expect = 1.282e-122
Identity = 279/750 (37.20%), Postives = 390/750 (52.00%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFN-YEGRSVQVAGWGSIAEDGSQ-SAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF---DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIKAGADTLVNSIGPGP--KSTPSHWAGGTP--VMGHCVTLKSCFP--LFNYQANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLY-PQYPGILGVPGAQGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVH------------------PGIPGVVQPPPPSYPVHAPIMTTPTTTTTTTTTTVWTTTTAP-------------TTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGST--RYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDR--WEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            RI+ GT + ++ +PF  +L  N R FCGGSLLD+ HILTAAHC+  +   ++ ++++ LG H L+       ++ ++K VVRHK + + + +ND+A+L +     F+ T+ P+CL   G  + Y  ++  V GWG++ E G      L++V V V +N +CR+SYG++APGGI  +M+CAA   +DSC GDSGGPL           Q+GIVSWGI     +   V  R  +    ++   T   +   +   V  +  G    S  + W   TP    G C +++SC P      Q +  S M   +  C  +EP  RQ + GVCC+ +Q   + +Y P + G  G P   GG  +P    P+ P    FG P F   G                      P        P  + P   P   T  + T+ TT+T   +TT+P              T S +     EPV +    +  CG G    +S  E        QR       R+V G  A +N WP+I AL   GR FCGGSLID++HILTAAHC+A MS+ D+  L V LG H ++   +S      V RVVRHKGF   TLYNDVA++ L +  P + S+ I PVCLP   T  ++  +  TVVGWG+LRE G QP+ LQ++T++I  NA C + Y   +P GI DHM+CA   G+DSCSGDSGGP +        W Q GIVSWGIGCGK  +PGVY+R T    WI +
Sbjct:  375 RIIGGTTSAKNAWPFAVSLMSNDRHFCGGSLLDEHHILTAAHCIAPLPTEELSEVEIILGLHTLKPLDPQIVRR-KLKRVVRHKGYHSDSFYNDIAILKMDSPVQFSDTISPVCLPSFGTTDLYVNKAAVVVGWGALQEGGKLLPKSLQQVTVRVQTNAECRKSYGSEAPGGILDNMLCAASPNKDSCMGDSGGPLVVQASPGSPWIQVGIVSWGI--VEDIKESVKGRSMDKSDVVKSQPTDEPVLGRSSKFVPMLPSGTFLLSDRTCW---TPDGKFGTCSSVRSCNPNVKLPLQKDVDSGMPSARGTCHYSEPDGRQ-VYGVCCAVKQAPSFGIYGPPFGGSFGFPANLGGPWWP----PYFP----FGGPSFPDVGTSPFIPPTTTTTRPSTTSPWWVPDPTTTTSRPGTTSPWWVPDQLTTKSPTSVTTSTNRPSTTSPWWVPESTTSTTSTPTTSTTSKPSVEPVVATDEGTRSCGRGPKKTLSLDE--------QR-------RIVGGTTALRNSWPFIVALFSRGRHFCGGSLIDASHILTAAHCIAHMSSTDISRLEVALGMHTLKPY-DSQALRKRVRRVVRHKGFDPVTLYNDVAILALDS--PVTFSNAISPVCLPPVGTTDQFVSKESTVVGWGALREGGRQPESLQQVTVQIQSNAECKRNYGKDAPGGIVDHMICAAYPGRDSCSGDSGGPMVTQEAPNSPWVQAGIVSWGIGCGKPPYPGVYSRVTSFLDWINK 1091          

HSP 2 Score: 182.185 bits (461), Expect = 2.557e-43
Identity = 118/298 (39.60%), Postives = 160/298 (53.69%), Query Frame = 0

Query:  612 TTTTTTTTTTVW------TTTTAPTTPSHSPVGQGEPVGSGSVSS---GECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTR--YTGRLGTVVGWGSLRENGP-QPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMI--GNGDRWEQVGIVSWGI 895
            TTT+  TTT+ W      +T   P   S  P  +   V   +VS    G CG G    I +                   R++ G  + KN WP+  +L+ + R FCGGSL+D  HILTAAHC+A +  +++  + + LG H + +  +       + RVVRHKG+ SD+ YND+A+  LK   P   SDTI PVCLPS  T   Y  +   VVGWG+L+E G   P  LQ++T+ +  NA C + Y   +P GI D+M+CA    KDSC GDSGGP ++    G  W QVGIVSWGI
Sbjct:  313 TTTSRPTTTSPWWFPDASSTLKLPVVTSTHPAIETSTVAMTTVSQSSGGSCGRGPFKLIEWP------------------RIIGGTTSAKNAWPFAVSLMSNDRHFCGGSLLDEHHILTAAHCIAPLPTEELSEVEIILGLHTL-KPLDPQIVRRKLKRVVRHKGYHSDSFYNDIAI--LKMDSPVQFSDTISPVCLPSFGTTDLYVNKAAVVVGWGALQEGGKLLPKSLQQVTVRVQTNAECRKSYGSEAPGGILDNMLCAASPNKDSCMGDSGGPLVVQASPGSPWIQVGIVSWGI 589          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1325331106|ref|XP_023347415.1| (trypsin-1-like isoform X3 [Eurytemora affinis])

HSP 1 Score: 364.385 bits (934), Expect = 1.579e-113
Identity = 185/374 (49.47%), Postives = 242/374 (64.71%), Query Frame = 0

Query:   98 LPQFQQPPVQAPLGNACVTNEGQNGTCTTVKECYPYFRLFNFDADETSTFGTYDTCSIDTRAGKKIFGICC-------VEKNIKADLLAEIEALRTEEPINPDEFPLDAQVNENCGKRS----------VGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCT--DRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILT 452
            L     PP+Q P    C+  EG  G C ++ ECYPYF++FN    +T   G YDTCS  +  G ++FG+CC        E+N   D   E + +         EFP++  +  NCG R+           G+    IVNG EA  HE+PFMAAL   +RQFCGGSL+D  HILTAAHCV H+S+NDV+ ++V LGDHN+++   A   + RVK V+RH  F +STLHNDVA+LTL E   +TPT+QPICL   G  ++  + V VAGWG+++E G+Q A L KV+VEVWSN++C+ SYG+ APGGIT  M+CA+   +DSCSGDSGGPLF C    +C QIGIVSWGIGCA+  +PGVYTRVTE+  WI +I+T
Sbjct:   54 LSPLLTPPLQQP----CLNKEGNVGRCKSLNECYPYFKIFNLSWRDTWVMGHYDTCSYRSPEGSQVFGVCCDSTPRSVTEEN--EDDNDETKFIDENNDAIVGEFPIEFALAGNCGFRTPQPSVTLYDKTGAQFY-IVNGAEAVPHEYPFMAALMNRNRQFCGGSLIDANHILTAAHCVAHLSKNDVQHLRVRLGDHNIKSNSAAGRVEKRVKRVIRHIGFSSSTLHNDVAILTLEEDVEYTPTIQPICLAA-GTNSFVNQRVSVAGWGTLSEGGTQPASLNKVEVEVWSNERCKNSYGSSAPGGITKEMLCASLPEQDSCSGDSGGPLFSCPYGGQCTQIGIVSWGIGCAKAQYPGVYTRVTEMRDWISRIVT 419          

HSP 2 Score: 254.218 bits (648), Expect = 4.348e-72
Identity = 122/236 (51.69%), Postives = 160/236 (67.80%), Query Frame = 0

Query:  684 VVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGP-FMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            +VNG  A  +E+P++AAL+   RQFCGGSLID+ HILTAAHCVA +S  DVQ L VRLG+HNI+    +   E  V RV+RH GFSS TL+NDVA++TL+  V    + TI+P+CL +G+  +  +  +V GWG+L E G QP  L ++ +E+W N RC   Y   +P GI   M+CA    +DSCSGDSGGP F    G +  Q+GIVSWGIGC KA +PGVYTR T+++ WI R
Sbjct:  183 IVNGAEAVPHEYPFMAALMNRNRQFCGGSLIDANHILTAAHCVAHLSKNDVQHLRVRLGDHNIKSNSAAGRVEKRVKRVIRHIGFSSSTLHNDVAILTLEEDV--EYTPTIQPICLAAGTNSFVNQRVSVAGWGTLSEGGTQPASLNKVEVEVWSNERCKNSYGSSAPGGITKEMLCASLPEQDSCSGDSGGPLFSCPYGGQCTQIGIVSWGIGCAKAQYPGVYTRVTEMRDWISR 416          

BLAST of trypsin-1 precursor vs. nr
Match: gi|225718884|gb|ACO15288.1| (Trypsin-1 precursor [Caligus clemensi])

HSP 1 Score: 349.747 bits (896), Expect = 1.671e-107
Identity = 181/390 (46.41%), Postives = 238/390 (61.03%), Query Frame = 0

Query:   99 PQFQQPPVQAPLGNACVTNEGQNGTCTTVKECYPYFRLFNF-DADETSTFGTYDTCSIDTRAGKKIFGICCV-------------------------EKNIKADLLAEIEALRTEE------------PINPDEFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKI 450
            PQ  + PV      AC+T  G+ G CT++K CYPYF++    + D T + G YD+CS     G+++FG+CC                          E    A+   + E   +EE              +PDE          C  R   +NL +IVNG +A  +E+PFMAALF  +R FCGGS++D  HILTAAHCV HM+++DVR ++V+LG+H++++  E   ++ RV+ ++RHKRF  STLHNDVA+LTL E+  +   +QPICL  D    YEG +V VAGWG+I E G QS  L+KVDV VW N +C  SYGN+APGGI +HM+CA+   +DSCSGDSGGPLF C   C Q+GIVSWGIGCAR  FPGVYTRVT L  WI+KI
Sbjct:   68 PQGAKDPV---FEQACLTRTGRLGQCTSLKVCYPYFKMPGAPNQDSTPSLGLYDSCSYVGEQGQQVFGVCCTGEQRLPPGEPTSRPTQPPKPTPPGEEATKDANDGMDFELHESEEGSVTDKEEDNGIRTSPDETANPKFNTGYCSARFYNNNL-KIVNGEDASLNEYPFMAALFNRNRHFCGGSIIDPKHILTAAHCVAHMTKSDVRHLRVHLGEHDIKSNYETGVRKLRVQRIIRHKRFSASTLHNDVAILTLRESVSYFDAIQPICLATDNSVRYEGDAVTVAGWGTIGEGGRQSRTLQKVDVTVWRNFECAASYGNRAPGGIQSHMLCASRPGKDSCSGDSGGPLFICEGVCTQVGIVSWGIGCAREQFPGVYTRVTALYSWIEKI 453          

HSP 2 Score: 253.062 bits (645), Expect = 2.552e-71
Identity = 124/240 (51.67%), Postives = 160/240 (66.67%), Query Frame = 0

Query:  682 LRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPS-GSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERAR 920
            L++VNG  A  NE+P++AAL    R FCGGS+ID  HILTAAHCVA M+  DV+ L V LGEH+I+   E+   +  V R++RHK FS+ TL+NDVA++TL+ +V  S  D I+P+CL +  S RY G   TV GWG++ E G Q   LQ++ + +W N  C   Y   +P GI+ HM+CA + GKDSCSGDSGGP  I  G    QVGIVSWGIGC +  FPGVYTR T +  WIE+ R
Sbjct:  218 LKIVNGEDASLNEYPFMAALFNRNRHFCGGSIIDPKHILTAAHCVAHMTKSDVRHLRVHLGEHDIKSNYETGVRKLRVQRIIRHKRFSASTLHNDVAILTLRESV--SYFDAIQPICLATDNSVRYEGDAVTVAGWGTIGEGGRQSRTLQKVDVTVWRNFECAASYGNRAPGGIQSHMLCASRPGKDSCSGDSGGPLFICEG-VCTQVGIVSWGIGCAREQFPGVYTRVTALYSWIEKIR 454          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1325331102|ref|XP_023347412.1| (transmembrane protease serine 9-like isoform X1 [Eurytemora affinis])

HSP 1 Score: 336.265 bits (861), Expect = 1.111e-101
Identity = 158/272 (58.09%), Postives = 198/272 (72.79%), Query Frame = 0

Query:  653 CGVGRHFPISYGENGEDVEAVQRIEGEHPL-RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            CGVG++  I Y +NG   E V+R+       ++VNG  A+ NEWPWIAAL+ +GRQFCGGSLID  HILTAAHCVA MS  DV  L +RLGEH I+  G++  FE+ V+RVVRHK FS  TL+ DVA++T+ + VP + +   RPVCLP+G   Y  R  TVVGWGSLRENGPQPD+LQE+T +IW+NA C + Y   +P GI+ HM+CAGK+G DSCSGDSGGP  IG G  W Q+GIVSWGIGCGK+ +PGVYTR T+V+ WI+R    +
Sbjct:  239 CGVGKYPVIRYAQNGSVAEEVERVTCNGSCSKIVNGNDAEDNEWPWIAALMNNGRQFCGGSLIDDKHILTAAHCVAHMSRYDVANLKIRLGEHKIKSGGDT-IFESKVSRVVRHKDFSQQTLHKDVAILTMADPVPSTFTRA-RPVCLPTGHELYADRTATVVGWGSLRENGPQPDILQEMTAKIWNNAECKETYGNAAPGGIEPHMLCAGKQGLDSCSGDSGGPMQIGEGQHWTQIGIVSWGIGCGKSFYPGVYTRVTEVRDWIDRIVKSY 508          

HSP 2 Score: 247.284 bits (630), Expect = 1.561e-68
Identity = 122/260 (46.92%), Postives = 170/260 (65.38%), Query Frame = 0

Query:  194 EFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPT-VQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPL-FDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
             +  +  V E   + +   +  +IVNG +A+ +E+P++AAL  N RQFCGGSL+D  HILTAAHCV HMS+ DV  +K+ LG+H +++ G+   +  +V  VVRHK F   TLH DVA+LT+++    T T  +P+CL   G   Y  R+  V GWGS+ E+G Q  IL+++  ++W+N +C+ +YGN APGGI  HM+CA     DSCSGDSGGP+         QIGIVSWGIGC +  +PGVYTRVTE+  WI +I+
Sbjct:  248 RYAQNGSVAEEVERVTCNGSCSKIVNGNDAEDNEWPWIAALMNNGRQFCGGSLIDDKHILTAAHCVAHMSRYDVANLKIRLGEHKIKSGGDTIFES-KVSRVVRHKDFSQQTLHKDVAILTMADPVPSTFTRARPVCLP-TGHELYADRTATVVGWGSLRENGPQPDILQEMTAKIWNNAECKETYGNAAPGGIEPHMLCAGKQGLDSCSGDSGGPMQIGEGQHWTQIGIVSWGIGCGKSFYPGVYTRVTEVRDWIDRIV 505          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1325331104|ref|XP_023347414.1| (transmembrane protease serine 9-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 335.88 bits (860), Expect = 1.236e-101
Identity = 158/272 (58.09%), Postives = 198/272 (72.79%), Query Frame = 0

Query:  653 CGVGRHFPISYGENGEDVEAVQRIEGEHPL-RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            CGVG++  I Y +NG   E V+R+       ++VNG  A+ NEWPWIAAL+ +GRQFCGGSLID  HILTAAHCVA MS  DV  L +RLGEH I+  G++  FE+ V+RVVRHK FS  TL+ DVA++T+ + VP + +   RPVCLP+G   Y  R  TVVGWGSLRENGPQPD+LQE+T +IW+NA C + Y   +P GI+ HM+CAGK+G DSCSGDSGGP  IG G  W Q+GIVSWGIGCGK+ +PGVYTR T+V+ WI+R    +
Sbjct:  238 CGVGKYPVIRYAQNGSVAEEVERVTCNGSCSKIVNGNDAEDNEWPWIAALMNNGRQFCGGSLIDDKHILTAAHCVAHMSRYDVANLKIRLGEHKIKSGGDT-IFESKVSRVVRHKDFSQQTLHKDVAILTMADPVPSTFTRA-RPVCLPTGHELYADRTATVVGWGSLRENGPQPDILQEMTAKIWNNAECKETYGNAAPGGIEPHMLCAGKQGLDSCSGDSGGPMQIGEGQHWTQIGIVSWGIGCGKSFYPGVYTRVTEVRDWIDRIVKSY 507          

HSP 2 Score: 246.899 bits (629), Expect = 1.602e-68
Identity = 122/260 (46.92%), Postives = 170/260 (65.38%), Query Frame = 0

Query:  194 EFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPT-VQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPL-FDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
             +  +  V E   + +   +  +IVNG +A+ +E+P++AAL  N RQFCGGSL+D  HILTAAHCV HMS+ DV  +K+ LG+H +++ G+   +  +V  VVRHK F   TLH DVA+LT+++    T T  +P+CL   G   Y  R+  V GWGS+ E+G Q  IL+++  ++W+N +C+ +YGN APGGI  HM+CA     DSCSGDSGGP+         QIGIVSWGIGC +  +PGVYTRVTE+  WI +I+
Sbjct:  247 RYAQNGSVAEEVERVTCNGSCSKIVNGNDAEDNEWPWIAALMNNGRQFCGGSLIDDKHILTAAHCVAHMSRYDVANLKIRLGEHKIKSGGDTIFES-KVSRVVRHKDFSQQTLHKDVAILTMADPVPSTFTRARPVCLP-TGHELYADRTATVVGWGSLRENGPQPDILQEMTAKIWNNAECKETYGNAAPGGIEPHMLCAGKQGLDSCSGDSGGPMQIGEGQHWTQIGIVSWGIGCGKSFYPGVYTRVTEVRDWIDRIV 504          

BLAST of trypsin-1 precursor vs. nr
Match: gi|909560120|ref|XP_013135138.1| (PREDICTED: proclotting enzyme-like isoform X1 [Papilio polytes])

HSP 1 Score: 335.495 bits (859), Expect = 4.943e-101
Identity = 203/470 (43.19%), Postives = 258/470 (54.89%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIP-FSPGQGG----FGTPG-----------FGGSG------IGVHPGIPGV-VQPPPPSYPVHAPIM-------TTPTTTTT-TTTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C   + +    + GVCC+                +G P  Q   +   G +P   P Q       G PG           FG +       I   P IP +   PP  + P H P +       T PTT+   +T +T W T    TT  T P   P    +P G  +V S  CG+ ++ P +YG   E       ++ E   R+V G  AD NEWPWIAAL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF+  TLYNDVA++TL    P   S  IRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW NA C   Y   +P GI +HM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   91 GTCKSFRDCYPLFKVVDLSGWDGWVMGHYDTCSFID-SNNMEVFGVCCT--------------APVGTPPQQEPDVQRLGALPPVLPAQAPPTQLVGFPGRIATSMSQQWTFGEANRERQIPINWPPTIPPLPTHPPDHTAPTHPPSIVAGNVEPTKPTTSRPPSTASTTWGTKPPSTTKQTWPPAYPTQPTKPAGQPAVDS-SCGI-KNGPQTYGTAYE-------LQDEE--RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDK--PVKFSKAIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNAECRLKYGSAAPGGIVEHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 532          

HSP 2 Score: 261.151 bits (666), Expect = 2.228e-73
Identity = 123/235 (52.34%), Postives = 160/235 (68.09%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH+ F   TL+NDVA+LTL +   F+  ++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  299 RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDKPVKFSKAIRPICLP-SGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNAECRLKYGSAAPGGIVEHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 532          

BLAST of trypsin-1 precursor vs. nr
Match: gi|910346044|ref|XP_013176913.1| (PREDICTED: proclotting enzyme-like isoform X2 [Papilio xuthus])

HSP 1 Score: 333.183 bits (853), Expect = 3.694e-100
Identity = 201/468 (42.95%), Postives = 250/468 (53.42%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIP-----FSPGQGGFGTPGFGGSGI---------GVHPGIPGVVQPPPPSYPVHAPIMTTPT----------TTTTT-----TTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C     +    + GVCC+                +G P  Q   +   G +P      +P       PG   S +              IP    P  P  P H P  T PT          T  TT     T +T W T    TT  T P   P    +P G  +V S  CG+ ++ P +YG   E       ++ E   R+V G  AD NEWPWIAAL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF+  TLYNDVA++TL    P   S TIRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW N  C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   93 GTCKSFRDCYPLFKVVDLSGWDGWVMGHYDTCSFIN-SNNMEVFGVCCT--------------APVGTPPQQEPDVQRLGALPPVLPAQAPPTQFLSFPGRIASSLTQQWTFGEANRERQIPANWPPTIPPLPTHPPDHTAPTHPPSIGNVEPTRPTTSRPPSTASTTWGTKPPSTTKQTWPPAYPTQPTKPTGQPAVDS-SCGI-KNGPQTYGTAYE-------LQDEE--RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDK--PVKFSKTIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 532          

HSP 2 Score: 262.307 bits (669), Expect = 7.982e-74
Identity = 124/235 (52.77%), Postives = 161/235 (68.51%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH+ F   TL+NDVA+LTL +   F+ T++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  299 RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDKPVKFSKTIRPICLP-SGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 532          

BLAST of trypsin-1 precursor vs. nr
Match: gi|910346042|ref|XP_013176912.1| (PREDICTED: proclotting enzyme-like isoform X1 [Papilio xuthus])

HSP 1 Score: 332.798 bits (852), Expect = 5.084e-100
Identity = 201/470 (42.77%), Postives = 250/470 (53.19%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIP-----FSPGQGGFGTPGFGGSGI---------GVHPGIPGVVQPPPPSYPVHAPIMTTPT------------TTTTT-----TTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C     +    + GVCC+                +G P  Q   +   G +P      +P       PG   S +              IP    P  P  P H P  T PT            T  TT     T +T W T    TT  T P   P    +P G  +V S  CG+ ++ P +YG   E       ++ E   R+V G  AD NEWPWIAAL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF+  TLYNDVA++TL    P   S TIRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW N  C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   93 GTCKSFRDCYPLFKVVDLSGWDGWVMGHYDTCSFIN-SNNMEVFGVCCT--------------APVGTPPQQEPDVQRLGALPPVLPAQAPPTQFLSFPGRIASSLTQQWTFGEANRERQIPANWPPTIPPLPTHPPDHTAPTHPPSIVAGNVEPTRPTTSRPPSTASTTWGTKPPSTTKQTWPPAYPTQPTKPTGQPAVDS-SCGI-KNGPQTYGTAYE-------LQDEE--RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDK--PVKFSKTIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 534          

HSP 2 Score: 262.692 bits (670), Expect = 7.978e-74
Identity = 124/235 (52.77%), Postives = 161/235 (68.51%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH+ F   TL+NDVA+LTL +   F+ T++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  301 RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDKPVKFSKTIRPICLP-SGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 534          

BLAST of trypsin-1 precursor vs. nr
Match: gi|930652485|gb|KPI94824.1| (Proclotting enzyme [Papilio xuthus])

HSP 1 Score: 332.028 bits (850), Expect = 5.337e-100
Identity = 201/470 (42.77%), Postives = 250/470 (53.19%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIP-----FSPGQGGFGTPGFGGSGI---------GVHPGIPGVVQPPPPSYPVHAPIMTTPT------------TTTTT-----TTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C     +    + GVCC+                +G P  Q   +   G +P      +P       PG   S +              IP    P  P  P H P  T PT            T  TT     T +T W T    TT  T P   P    +P G  +V S  CG+ ++ P +YG   E       ++ E   R+V G  AD NEWPWIAAL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF+  TLYNDVA++TL    P   S TIRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW N  C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   77 GTCKSFRDCYPLFKVVDLSGWDGWVMGHYDTCSFIN-SNNMEVFGVCCT--------------APVGTPPQQEPDVQRLGALPPVLPAQAPPTQFLSFPGRIASSLTQQWTFGEANRERQIPANWPPTIPPLPTHPPDHTAPTHPPSIVAGNVEPTRPTTSRPPSTASTTWGTKPPSTTKQTWPPAYPTQPTKPTGQPAVDS-SCGI-KNGPQTYGTAYE-------LQDEE--RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDK--PVKFSKTIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 518          

HSP 2 Score: 262.692 bits (670), Expect = 5.370e-74
Identity = 124/235 (52.77%), Postives = 161/235 (68.51%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH+ F   TL+NDVA+LTL +   F+ T++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  285 RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDKPVKFSKTIRPICLP-SGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 518          

BLAST of trypsin-1 precursor vs. nr
Match: gi|943945785|ref|XP_014356121.1| (PREDICTED: LOW QUALITY PROTEIN: proclotting enzyme-like [Papilio machaon])

HSP 1 Score: 327.791 bits (839), Expect = 3.203e-98
Identity = 197/466 (42.27%), Postives = 249/466 (53.43%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCST------------------RQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVHPGIPGV-VQPPPPSYPVHAPIM-------TTPTTTT-TTTTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C     +    + GVCC+                    ++    L   +PG +     Q      A      P                  P IP +   PP  + P H P +       T PTT+  ++T +T W T    TT  T     P    +P G  +V S  CG+ ++ P +YG   E       I+ E   R+V G  AD NEWPWI AL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH GF+  TLYNDVA++TL    P   S TIRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW NA C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   93 GTCKSFRDCYPLFKVVDLSGWDGWVMGHYDTCSFIN-SNNMEVFGVCCTAPVGTPSSTNFCKVNXIVKXKYLSKMLXCSFPGRIASSLTQQWTFGEANRERQIPANW--------------PPTIPPLPTHPPDHAAPTHPPSIVAGNVEPTRPTTSKPSSTASTTWGTKPPSTTKQTWSPAYPTKPTKPTGQPAVDS-SCGI-KNGPQTYGTAYE-------IQDEE--RIVGGHNADLNEWPWIVALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHHGFNMQTLYNDVAILTLDK--PVKFSKTIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNAECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 530          

HSP 2 Score: 259.61 bits (662), Expect = 9.333e-73
Identity = 123/235 (52.34%), Postives = 159/235 (67.66%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++ ALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH  F   TL+NDVA+LTL +   F+ T++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  297 RIVGGHNADLNEWPWIVALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHHGFNMQTLYNDVAILTLDKPVKFSKTIRPICLPS-GNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNAECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 530