BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000012449 (supercontig:LSalAtl2s:LSalAtl2s914:71788:76892:-1 gene:EMLSAG00000012449 transcript:EMLSAT00000012449 description:"maker-LSalAtl2s914-augustus-gene-0.19")

HSP 1 Score: 139.428 bits (350), Expect = 6.043e-34
Identity = 92/288 (31.94%), Postives = 147/288 (51.04%), Query Frame = 0

Query:  659 FP--ISYGENGEDVEAVQRIEGEHPL---------------RVVNGWPADKNEWPWIAALLRSGRQF-CGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVR------HKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPS-GSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAG--IKDHMMCAGK-KGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            FP  +  G N   +   QR   EHP+               RVV G  A   EW W  AL+ S  Q+ CGG+LI +  +LTAAHCV  +  +    + VR+G+H++ ++     F +P  + +R      H   +S TL ND+A++ L       + + +  VCLP+ G+++  G++ TV G+G + E+GP P  ++E  + + ++  C +    V+     +     CAG  +G D+C GD GGP +  +   +   G+VSWG GCG+ D PGVY + +   GWI +
Sbjct:  649 FPSNVGSGVNKFHIAKEQRPTLEHPVTNKYVCGVKGTYRSGRVVGGDDASPGEWCWQVALINSLNQYLCGGALIGTQWVLTAAHCVTNI-VRAGDSIYVRVGDHDLTQK-----FGSPGAQTLRVATTYIHHNHNSQTLDNDIAILKLHGEA--DLKEGVCLVCLPARGTSQQAGKVCTVTGYGYMGESGPIPLRVREAQVPVVNDNECVRKINAVTEKIFILPASSFCAGGLEGHDACQGDGGGPLVCEDSGYYXLTGLVSWGFGCGRRDVPGVYVKISSFIGWINQ 928          

HSP 2 Score: 125.176 bits (313), Expect = 1.709e-29
Identity = 81/244 (33.20%), Postives = 121/244 (49.59%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQF-CGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTR-GEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYG--NQAPGGITTHMICAAGTF-RDSCSGDSGGPLFDCTDR--CRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILT 452
            R+V G +A   E+ +  AL  +  Q+ CGG+L+    +LTAAHCV ++ +     + V +GDH+L  + G    Q  RV T   H    + TL ND+A+L L   A     V  +CL   G     G+   V G+G + E G     +R+  V V ++++C R      +    +     CA G    D+C GD GGPL  C D       G+VSWG GC R   PGVY +++  + WI +I++
Sbjct:  690 RVVGGDDASPGEWCWQVALINSLNQYLCGGALIGTQWVLTAAHCVTNIVRAG-DSIYVRVGDHDLTQKFGSPGAQTLRVATTYIHHNHNSQTLDNDIAILKLHGEADLKEGVCLVCLPARGTSQQAGKVCTVTGYGYMGESGPIPLRVREAQVPVVNDNECVRKINAVTEKIFILPASSFCAGGLEGHDACQGDGGGPLV-CEDSGYYXLTGLVSWGFGCGRRDVPGVYVKISSFIGWINQIIS 931          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000002524 (supercontig:LSalAtl2s:LSalAtl2s149:678275:680326:1 gene:EMLSAG00000002524 transcript:EMLSAT00000002524 description:"maker-LSalAtl2s149-augustus-gene-6.17")

HSP 1 Score: 129.413 bits (324), Expect = 4.469e-33
Identity = 87/265 (32.83%), Postives = 137/265 (51.70%), Query Frame = 0

Query:  206 GKRSVGSNLVRIVNGTEAKQHEFPFMA----ALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICL--EQDGLFNYEGRSVQ-----------VAGWGSIAEDGSQSA-ILRKVDVEVWSN--DKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPL-FDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            G  S GS   RI+ G   K++E P+        F    + CGGS++ + +ILTAAHC+ H    +   ++V LG  N  +   + E+ + +++   H  +      ND+A+L L     F   V+PICL    D LF  +   ++           V+GWG+ +E+  +S+ ++++  + V  N  DKCR +   + P  ++   ICA GT  D+C  DSGGPL     + C   GIVS+G GCA+  F GVYTRV+  + WI++
Sbjct:   48 GAMSCGSREGRIIGGRVVKENEIPWQVNIEMCFFDICERLCGGSVIGRKYILTAAHCILH----NTHHLQVSLGLLN-DSNPISDERVYSIESTKLHNDWNAVGAQNDIALLKLDRDIEFDTAVRPICLPSNPDNLFAKKPADIRRCNPGPLMVLCVSGWGTTSEENYESSPVMKRTILRVLENGDDKCRNNDPYRRP--LSNKYICAFGTDTDACHIDSGGPLTLVENNTCSLAGIVSFGNGCAKKDFAGVYTRVSAHLDWIRE 305          

HSP 2 Score: 96.2857 bits (238), Expect = 7.563e-22
Identity = 72/256 (28.12%), Postives = 109/256 (42.58%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIA----ALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRRE-GESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGR--------------LGTVVGWGSL-RENGPQPDVLQELTMEIWDNARCDQIYQGVSPAG--IKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            R++ G    +NE PW             + CGGS+I   +ILTAAHC+      +   L V LG  N      +   +    T++  H  +++    ND+AL+ L   +       +RP+CLPS       +              +  V GWG+   EN     V++   + + +N   D   +   P    + +  +CA     D+C  DSGGP  +   +     GIVS+G GC K DF GVYTR +    WI
Sbjct:   58 RIIGGRVVKENEIPWQVNIEMCFFDICERLCGGSVIGRKYILTAAHCILH----NTHHLQVSLGLLNDSNPISDERVYSIESTKL--HNDWNAVGAQNDIALLKLDRDI--EFDTAVRPICLPSNPDNLFAKKPADIRRCNPGPLMVLCVSGWGTTSEENYESSPVMKRTILRVLENG--DDKCRNNDPYRRPLSNKYICAFGTDTDACHIDSGGPLTLVENNTCSLAGIVSFGNGCAKKDFAGVYTRVSAHLDWI 303          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000001293 (supercontig:LSalAtl2s:LSalAtl2s1214:59320:63000:-1 gene:EMLSAG00000001293 transcript:EMLSAT00000001293 description:"maker-LSalAtl2s1214-augustus-gene-0.47")

HSP 1 Score: 133.265 bits (334), Expect = 5.754e-32
Identity = 83/238 (34.87%), Postives = 129/238 (54.20%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAAL----FKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDS-CSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQ 448
            RI+ G +A+ H FPF+A+L    F NS  +C GS+L++ +ILT  +C   +   D   +++ +G+H+L       EQ F+VK ++ H+ +   TL ND+ +L       F   V    L   GL  +E  +  ++GWGS++ D S S +L+ + VE+ SN+ C   Y           M+CA+       C GDSGGPL  C D   Q G++SW I C +  +PGV+TR+ + + WIQ
Sbjct:  642 RIIGGNDARPHAFPFIASLKDVRFANS-HYCAGSVLNENYILTTGNCCHKV---DSHYIQIGVGEHHLYQTDH--EQFFKVKKMIVHENYDKITLKNDICLLKADRNIKFNSYVSTTKLPNLGL-AFENETGIISGWGSLSLDLSPSNVLQFLTVEIMSNELCSELYMEYYDPKT---MMCASSIENSGFCLGDSGGPLI-CDD-FYQCGMISWNIKCGKNNYPGVFTRLGDYVNWIQ 867          

HSP 2 Score: 125.561 bits (314), Expect = 1.221e-29
Identity = 75/245 (30.61%), Postives = 127/245 (51.84%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAAL--LR-SGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIY-QGVSPAGIKDHMMCAGK-KGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNK 922
            R++ G  A  + +P+IA+L  +R +   +C GS+++  +ILT  +C  ++ +  +Q   + +GEH++ +      F+  V +++ H+ +   TL ND+ L+     +    +  +    LP+    +    G + GWGSL  +    +VLQ LT+EI  N  C ++Y +   P      MMCA   +    C GDSGGP +    D + Q G++SW I CGK ++PGV+TR  D   WI+    K
Sbjct:  642 RIIGGNDARPHAFPFIASLKDVRFANSHYCAGSVLNENYILTTGNCCHKVDSHYIQ---IGVGEHHLYQTDHEQFFK--VKKMIVHENYDKITLKNDICLLKADRNIK--FNSYVSTTKLPNLGLAFENETGIISGWGSLSLDLSPSNVLQFLTVEIMSNELCSELYMEYYDPKT----MMCASSIENSGFCLGDSGGPLI---CDDFYQCGMISWNIKCGKNNYPGVFTRLGDYVNWIQDNMEK 872          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000005416 (supercontig:LSalAtl2s:LSalAtl2s2918:1117:2218:1 gene:EMLSAG00000005416 transcript:EMLSAT00000005416 description:"maker-LSalAtl2s2918-snap-gene-0.4")

HSP 1 Score: 122.479 bits (306), Expect = 1.327e-31
Identity = 73/197 (37.06%), Postives = 113/197 (57.36%), Query Frame = 0

Query:  253 LTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            +TAAHC  ++ +N     KV  GDH+ +   E  EQ+ RVK+ V H  F TS ++ND+ +L L +   F    +  CL +  +  +EG +V ++GWG+       S + R   V + S  +C+ +YG+     +   MICAA   +DSC GDSGGP+     +C   G+VSWG GCA P +PGVYT++++ M W+++
Sbjct:   51 ITAAHCCRNLPKN----AKVVAGDHS-QLSLEGFEQRIRVKSYVIHPDFGTSGVNNDICILHLEKPLKFN---ELQCLTK--IXXFEGDAV-ISGWGTTYSGAPPSFVXRWAKVNIVSKAECQNAYGSL----LDDSMICAAAPGKDSCQGDSGGPMVCDGVQC---GVVSWGYGCAAPKYPGVYTKLSKFMDWVKE 229          

HSP 2 Score: 86.6557 bits (213), Expect = 3.254e-19
Identity = 61/212 (28.77%), Postives = 92/212 (43.40%), Query Frame = 0

Query:  713 LIDSTHILTAAHCV------AQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            +I     +TAAHC       A++ A D   L++   E  IR           V   V H  F +  + ND+ ++ L+  +  +    +  +    G          + GWG+     P   V +   + I   A C   Y  +    + D M+CA   GKDSC GDSGGP M+ +G    Q G+VSWG GC    +PGVYT+ +    W++ 
Sbjct:   44 IISVKTAITAAHCCRNLPKNAKVVAGDHSQLSLEGFEQRIR-----------VKSYVIHPDFGTSGVNNDICILHLEKPLKFNELQCLTKIXXFEGD-------AVISGWGTTYSGAPPSFVXRWAKVNIVSKAECQNAYGSL----LDDSMICAAAPGKDSCQGDSGGP-MVCDG---VQCGVVSWGYGCAAPKYPGVYTKLSKFMDWVKE 229          

BLAST of trypsin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000001033 (supercontig:LSalAtl2s:LSalAtl2s1171:175996:177422:1 gene:EMLSAG00000001033 transcript:EMLSAT00000001033 description:"maker-LSalAtl2s1171-augustus-gene-1.27")

HSP 1 Score: 124.02 bits (310), Expect = 1.726e-31
Identity = 85/255 (33.33%), Postives = 128/255 (50.20%), Query Frame = 0

Query:  207 KRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAED---GSQSAILRKVDVEVWSNDKCRRSYGNQ-APGGITTHMICAAGTF--RDSCSGDSGGPLFDCTD-RCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPL 454
            KRS G+   R + G E    ++PF  ++ KN    CGG+++ + HILTAAHCV    +   +   V +G+ N+      A+   RV  +  H  +K  +  +D A+L LS    ++   QP CL  D L    G   ++AGWG   E    G     L  V + +  N+ C+  + +Q  P  I    +CA      +D C GDSGG L    + + +  G++S GIGC R   PGVYTRV+  M WI+K++T +
Sbjct:   42 KRSGGA---RXLGGFETPILDYPFAVSIRKNGAHHCGGAIIGRRHILTAAHCVHSYPE---KVWSVVVGESNVDR--PPAQSPIRVSKLHIHHNYKRPSYTHDXAILXLSSDIKWSDKSQPACLSDDELV--VGSDGELAGWGYDNEKRKGGKPMNTLHSVKLPILENEVCQSWFSSQNKPIVIEDTHMCAGYEEGGKDGCQGDSGGGLVVKEEGKFKLTGVMSAGIGCGRAKLPGVYTRVSSYMDWIRKVVTEV 286          

HSP 2 Score: 121.709 bits (304), Expect = 1.096e-30
Identity = 76/243 (31.28%), Postives = 124/243 (51.03%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWG---SLRENGPQPDVLQELTMEIWDNARCDQIYQGVS-PAGIKDHMMCAGKK--GKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERA 919
            R + G+     ++P+  ++ ++G   CGG++I   HILTAAHCV     +   + +V +GE N+ R    +     V+++  H  +   +  +D A++ L + +    SD  +P CL S      G  G + GWG     R+ G   + L  + + I +N  C   +   + P  I+D  MCAG +  GKD C GDSGG  ++    +++  G++S GIGCG+A  PGVYTR +    WI + 
Sbjct:   48 RXLGGFETPILDYPFAVSIRKNGAHHCGGAIIGRRHILTAAHCVHSYPEK---VWSVVVGESNVDRPPAQSPIR--VSKLHIHHNYKRPSYTHDXAILXLSSDI--KWSDKSQPACL-SDDELVVGSDGELAGWGYDNEKRKGGKPMNTLHSVKLPILENEVCQSWFSSQNKPIVIEDTHMCAGYEEGGKDGCQGDSGGGLVVKEEGKFKLTGVMSAGIGCGRAKLPGVYTRVSSYMDWIRKV 282          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|129688|sp|P21902.1|PCE_TACTR (RecName: Full=Proclotting enzyme; Contains: RecName: Full=Proclotting enzyme light chain; Contains: RecName: Full=Proclotting enzyme heavy chain; Flags: Precursor)

HSP 1 Score: 168.318 bits (425), Expect = 2.159e-44
Identity = 97/250 (38.80%), Postives = 140/250 (56.00%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAAL------LRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQ---MLTVRLGEHNIRR-EGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTG---RLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMI-GNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            R++ G  A    WPW+ A+      +RS +  CGG+L+ + H++TA+HCV   +  DV    + +VRLGEHN+   + +SN  +  VT V  H+ F   T  ND+A++TL + V  + +D IRP+CLP    RY     R   + GWG+   NGP   VL+E+ + IW++  C Q Y+      I +  MCAG    GKD+C GDSGGP M+      +  +GIVS+G  C    FPGVYT+ T+   WI
Sbjct:  127 RIIGGREAPIGAWPWMTAVYIKQGGIRSVQ--CGGALVTNRHVITASHCVVNSAGTDVMPADVFSVRLGEHNLYSTDDDSNPIDFAVTSVKHHEHFVLATYLNDIAILTLNDTV--TFTDRIRPICLPYRKLRYDDLAMRKPFITGWGTTAFNGPSSAVLREVQLPIWEHEACRQAYE--KDLNITNVYMCAGFADGGKDACQGDSGGPMMLPVKTGEFYLIGIVSFGKKCALPGFPGVYTKVTEFLDWI 370          

HSP 2 Score: 163.696 bits (413), Expect = 9.517e-43
Identity = 116/352 (32.95%), Postives = 181/352 (51.42%), Query Frame = 0

Query:  114 CVTNEGQNGTCTTVKECYPYFRLFNFDADETSTFGTYDTCSIDTRAGKKIFGICCVEK-NIKADLLAEIEALRTEEPINPDEFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQF----CGGSLLDKTHILTAAHCVEHMSQNDVRQMKVY---LGDHNL-RTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQ---VAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICA--AGTFRDSCSGDSGGPLF--DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            C     + GTC  V +C    +  +++  + S       C  +    K    +CC +  ++ +   A  E   TE P  P + P    + E CG  +  +   RI+ G EA    +P+M A++          CGG+L+   H++TA+HCV + +  DV    V+   LG+HNL  T  ++    F V +V  H+ F  +T  ND+A+LTL++   FT  ++PICL    L  Y+  +++   + GWG+ A +G  SA+LR+V + +W ++ CR++Y       IT   +CA  A   +D+C GDSGGP+     T     IGIVS+G  CA P FPGVYT+VTE + WI +
Sbjct:   40 CSNRFTEEGTCKNVLDCRILLQKNDYNLLKESI------CGFEGITPK----VCCPKSSHVISSTQAPPETTTTERP--PKQIP--PNLPEVCGIHNTTTT--RIIGGREAPIGAWPWMTAVYIKQGGIRSVQCGGALVTNRHVITASHCVVNSAGTDVMPADVFSVRLGEHNLYSTDDDSNPIDFAVTSVKHHEHFVLATYLNDIAILTLNDTVTFTDRIRPICLPYRKL-RYDDLAMRKPFITGWGTTAFNGPSSAVLREVQLPIWEHEACRQAYEKDL--NITNVYMCAGFADGGKDACQGDSGGPMMLPVKTGEFYLIGIVSFGKKCALPGFPGVYTKVTEFLDWIAE 372          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|18202734|sp|Q9BQR3.1|PRS27_HUMAN (RecName: Full=Serine protease 27; AltName: Full=Marapsin; AltName: Full=Pancreasin; Flags: Precursor)

HSP 1 Score: 160.229 bits (404), Expect = 1.359e-42
Identity = 99/257 (38.52%), Postives = 132/257 (51.36%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYN------DVALITLKNAVPESMSDTIRPVCLPSGSTRY-TGRLGTVVGWGSLRENG--PQPDVLQELTMEIWDNARCDQIYQ-----GVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            R+V G    + EWPW  ++ R+G  FCGGSLI    +LTAAHC    S  +  +  V LG   + + G       P     R +   S+ LY       DVAL+ L+  VP   ++ I PVCLP  S  + TG    V GWGS  E    P+P +LQ+L + I D  +C+ +Y      G  P  IK+ M+CAG  +  KD+C GDSGGP +   G  W Q G++SWG GC + + PGVY R T    WI R   K 
Sbjct:   34 RMVGGQDTQEGEWPWQVSIQRNGSHFCGGSLIAEQWVLTAAHCFRNTS--ETSLYQVLLGARQLVQPG-------PHAMYARVRQVESNPLYQGTASSADVALVELEAPVP--FTNYILPVCLPDPSVIFETGMNCWVTGWGSPSEEDLLPEPRILQKLAVPIIDTPKCNLLYSKDTEFGYQPKTIKNDMLCAGFEEGKKDACKGDSGGPLVCLVGQSWLQAGVISWGEGCARQNRPGVYIRVTAHHNWIHRIIPKL 279          

HSP 2 Score: 136.346 bits (342), Expect = 3.365e-34
Identity = 90/269 (33.46%), Postives = 134/269 (49.81%), Query Frame = 0

Query:  199 AQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDG--SQSAILRKVDVEVWSNDKCRRSYGNQA-----PGGITTHMICAAGT--FRDSCSGDSGGPLFDCTDRC-RQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIK 457
            A+    CG+  +   L R+V G + ++ E+P+  ++ +N   FCGGSL+ +  +LTAAHC  + S+  + Q  V LG   L   G  A    RV+ V  +  ++ +    DVA++ L     FT  + P+CL    +    G +  V GWGS +E+    +  IL+K+ V +    KC   Y         P  I   M+CA      +D+C GDSGGPL     +   Q G++SWG GCAR   PGVY RVT    WI +I+  L  +
Sbjct:   20 AKAATACGRPRM---LNRMVGGQDTQEGEWPWQVSIQRNGSHFCGGSLIAEQWVLTAAHCFRNTSETSLYQ--VLLGARQLVQPGPHA-MYARVRQVESNPLYQGTASSADVALVELEAPVPFTNYILPVCLPDPSVIFETGMNCWVTGWGSPSEEDLLPEPRILQKLAVPIIDTPKCNLLYSKDTEFGYQPKTIKNDMLCAGFEEGKKDACKGDSGGPLVCLVGQSWLQAGVISWGEGCARQNRPGVYIRVTAHHNWIHRIIPKLQFQ 282          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|78100736|sp|Q6BEA2.1|PRS27_RAT (RecName: Full=Serine protease 27; AltName: Full=Marapsin; AltName: Full=Pancreasin; Flags: Precursor)

HSP 1 Score: 160.999 bits (406), Expect = 2.156e-42
Identity = 96/246 (39.02%), Postives = 133/246 (54.07%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRY-TGRLGTVVGWGSLRENG--PQPDVLQELTMEIWDNARCDQIYQGVSPAGI-----KDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            R+V G  A + EWPW  ++ R+G  FCGGSLI  T +LTAAHC +  S  D+ +  V LG   +++ G    +  PV RV  H  +       DVAL+ L+  VP + +  I PVCLP  S  + +G    V GWGS  E    P P +LQ+L + + D  +C+ +Y   + A I     KD M+CAG  +  KD+C GDSGGP +      W Q G++SWG GC + + PGVY R      WI +
Sbjct:   37 RMVGGEDALEGEWPWQVSIQRNGAHFCGGSLIAPTWVLTAAHCFSNTS--DISIYQVLLGALKLQQPGPHALY-VPVKRVKSHPEYQGMASSADVALVELQ--VPVTFTKYILPVCLPDPSVVFKSGMNCWVTGWGSPSEQDRLPNPRILQKLAVPLIDTPKCNLLYSKDAEADIQLKTIKDDMLCAGFAEGKKDACKGDSGGPLVCLVDQSWVQAGVISWGEGCARRNRPGVYIRVASHYQWIHQ 277          

HSP 2 Score: 137.887 bits (346), Expect = 2.059e-34
Identity = 91/255 (35.69%), Postives = 126/255 (49.41%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGS--QSAILRKVDVEVWSNDKCRRSYGNQAPGGITTH-----MICA--AGTFRDSCSGDSGGPLFDCTDRCR-QIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIKAGA 460
            R+V G +A + E+P+  ++ +N   FCGGSL+  T +LTAAHC  + S  D+   +V LG   L+  G  A     VK V  H  ++      DVA++ L     FT  + P+CL    +    G +  V GWGS +E        IL+K+ V +    KC   Y   A   I        M+CA  A   +D+C GDSGGPL    D+   Q G++SWG GCAR   PGVY RV     WI +I+  L  +  A
Sbjct:   37 RMVGGEDALEGEWPWQVSIQRNGAHFCGGSLIAPTWVLTAAHCFSNTS--DISIYQVLLGALKLQQPGPHA-LYVPVKRVKSHPEYQGMASSADVALVELQVPVTFTKYILPVCLPDPSVVFKSGMNCWVTGWGSPSEQDRLPNPRILQKLAVPLIDTPKCNLLYSKDAEADIQLKTIKDDMLCAGFAEGKKDACKGDSGGPLVCLVDQSWVQAGVISWGEGCARRNRPGVYIRVASHYQWIHQIIPELQFQGRA 288          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|78100735|sp|Q8BJR6.1|PRS27_MOUSE (RecName: Full=Serine protease 27; AltName: Full=Marapsin; AltName: Full=Pancreasin; Flags: Precursor)

HSP 1 Score: 156.762 bits (395), Expect = 6.830e-41
Identity = 95/246 (38.62%), Postives = 132/246 (53.66%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRY-TGRLGTVVGWGSLRENG--PQPDVLQELTMEIWDNARCDQIYQ-----GVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            R+V G  A + EWPW  ++ R+G  FCGGSLI  T +LTAAHC +  S  D+ +  V LG   +++ G    +  PV +V  +  +       DVAL+ L+   P + ++ I PVCLP  S  + +G    V GWGS  E    P P VLQ+L + I D  +C+ +Y            IKD M+CAG  +  KD+C GDSGGP +      W Q G++SWG GC + + PGVY R T    WI +
Sbjct:   37 RMVGGENALEGEWPWQVSIQRNGIHFCGGSLIAPTWVLTAAHCFSNTS--DISIYQVLLGALKLQQPGPHALY-VPVKQVKSNPQYQGMASSADVALVELQG--PVTFTNYILPVCLPDPSVIFESGMNCWVTGWGSPSEQDRLPNPRVLQKLAVPIIDTPKCNLLYNKDVESDFQLKTIKDDMLCAGFAEGKKDACKGDSGGPLVCLVDQSWVQAGVISWGEGCARRNRPGVYIRVTSHHKWIHQ 277          

HSP 2 Score: 134.035 bits (336), Expect = 4.267e-33
Identity = 90/257 (35.02%), Postives = 127/257 (49.42%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGS--QSAILRKVDVEVWSNDKCRRSYGNQAPGG-----ITTHMICA--AGTFRDSCSGDSGGPLFDCTDRC-RQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIKAGADT 462
            R+V G  A + E+P+  ++ +N   FCGGSL+  T +LTAAHC  + S  D+   +V LG   L+  G  A     VK V  + +++      DVA++ L     FT  + P+CL    +    G +  V GWGS +E        +L+K+ V +    KC   Y            I   M+CA  A   +D+C GDSGGPL    D+   Q G++SWG GCAR   PGVY RVT    WI +I+  L  +  A T
Sbjct:   37 RMVGGENALEGEWPWQVSIQRNGIHFCGGSLIAPTWVLTAAHCFSNTS--DISIYQVLLGALKLQQPGPHA-LYVPVKQVKSNPQYQGMASSADVALVELQGPVTFTNYILPVCLPDPSVIFESGMNCWVTGWGSPSEQDRLPNPRVLQKLAVPIIDTPKCNLLYNKDVESDFQLKTIKDDMLCAGFAEGKKDACKGDSGGPLVCLVDQSWVQAGVISWGEGCARRNRPGVYIRVTSHHKWIHQIIPELQFQGRAGT 290          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|338817867|sp|Q8BZ10.2|TM11G_MOUSE (RecName: Full=Transmembrane protease serine 11G; AltName: Full=Serine protease DESC4; Contains: RecName: Full=Transmembrane protease serine 11G non-catalytic chain; Contains: RecName: Full=Transmembrane protease serine 11G catalytic chain; Flags: Precursor)

HSP 1 Score: 159.073 bits (401), Expect = 7.554e-41
Identity = 92/239 (38.49%), Postives = 135/239 (56.49%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVV-GWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG-KKGK-DSCSGDSGGPFMIG-NGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIE 917
            R+ +G PADK  WPW ++L   G   CG SLI S  ++T+AHC      ++ ++ TV  G     R   S      V  ++ H+ ++S    +D+A++ L +  P   S+ +  VCLP  + +   +    V GWG+L+ NGP P+ LQE+ +EI  N  C+Q+   V    I   M+CAG   GK D+C GDSGGP +I  N ++W  +GIVSWGI CGK + PG+YTR T  + WI+
Sbjct:  185 RIADGKPADKASWPWQSSLQVEGIHLCGASLIGSQWLVTSAHCFDNY--KNPKLWTVSFG-----RTLSSPLTTRKVESIIVHENYASHKHDDDIAVVKLSS--PVLFSENLHRVCLPDATFQVLPKSKVFVTGWGALKANGPFPNSLQEVEIEIISNDVCNQV--NVYGGAISSGMICAGFLTGKLDACEGDSGGPLVISDNRNKWYLLGIVSWGIDCGKENKPGIYTRVTHYRDWIK 412          

HSP 2 Score: 125.946 bits (315), Expect = 1.322e-29
Identity = 82/254 (32.28%), Postives = 134/254 (52.76%), Query Frame = 0

Query:  201 VNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRS-VQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICA---AGTFRDSCSGDSGGPLF--DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQ 448
            +N +CG       + RI +G  A +  +P+ ++L       CG SL+    ++T+AHC ++    + +   V  G    RT       + +V++++ H+ + +    +D+AV+ LS   +F+  +  +CL  D  F    +S V V GWG++  +G     L++V++E+ SND C +   N   G I++ MICA    G   D+C GDSGGPL   D  ++   +GIVSWGI C +   PG+YTRVT    WI+
Sbjct:  170 LNSDCGSGMEYPPIARIADGKPADKASWPWQSSLQVEGIHLCGASLIGSQWLVTSAHCFDNY--KNPKLWTVSFG----RTLSSPLTTR-KVESIIVHENYASHKHDDDIAVVKLSSPVLFSENLHRVCLP-DATFQVLPKSKVFVTGWGALKANGPFPNSLQEVEIEIISNDVCNQV--NVYGGAISSGMICAGFLTGKL-DACEGDSGGPLVISDNRNKWYLLGIVSWGIDCGKENKPGIYTRVTHYRDWIK 412          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|68051955|sp|Q5QSK2.1|TM11G_RAT (RecName: Full=Transmembrane protease serine 11G; AltName: Full=Serine protease DESC4; Contains: RecName: Full=Transmembrane protease serine 11G non-catalytic chain; Contains: RecName: Full=Transmembrane protease serine 11G catalytic chain; Flags: Precursor)

HSP 1 Score: 158.688 bits (400), Expect = 1.202e-40
Identity = 99/278 (35.61%), Postives = 147/278 (52.88%), Query Frame = 0

Query:  648 VSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVV-GWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG-KKGK-DSCSGDSGGPFMIG-NGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARN 921
            + +  CG+G  +P              RI      R+ +G PA  N WPW ++L   G   CG SLI S  ++T+AHC      ++ ++ TV  G    R  G   T    V  ++ H+ +++    +D+A++ L +  P   S+ +R VCLP  + +   +    V GWG+L+ NGP P+ LQE+ +EI  N  C+Q+   V    I   M+CAG   GK D+C GDSGGP +I  N ++W  +GIVSWGI CGK + PG+YTR T  + WI+   N
Sbjct:  169 ILNSNCGLGMEYP--------------RIA-----RIADGKPAGSNSWPWQSSLQVEGIHLCGASLIGSQWLVTSAHCFDNY--KNPKLWTVSFG----RTLGNPLT-TRKVESIIIHENYAAHKHDDDIAVVKLSS--PVLFSENLRTVCLPEATFQVLPKSKVFVTGWGALKANGPFPNSLQEVEIEIISNDVCNQV--NVYGGAISSGMICAGFLTGKLDACEGDSGGPLVISDNRNKWYLLGIVSWGIDCGKENKPGIYTRVTHYRNWIKSKTN 416          

HSP 2 Score: 129.413 bits (324), Expect = 7.867e-31
Identity = 86/285 (30.18%), Postives = 143/285 (50.18%), Query Frame = 0

Query:  169 VEKNIKADLLAEIEALRTEEPINPDEFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICA---AGTFRDSCSGDSGGPLF--DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQ 448
            ++++I    L E+ A + E  +N            NCG       + RI +G  A  + +P+ ++L       CG SL+    ++T+AHC ++    + +   V  G    RT G     + +V++++ H+ +      +D+AV+ LS   +F+  ++ +CL +          V V GWG++  +G     L++V++E+ SND C +   N   G I++ MICA    G   D+C GDSGGPL   D  ++   +GIVSWGI C +   PG+YTRVT    WI+
Sbjct:  149 LKRDISLPYLREMNAAQAEHILN-----------SNCGLGMEYPRIARIADGKPAGSNSWPWQSSLQVEGIHLCGASLIGSQWLVTSAHCFDNYK--NPKLWTVSFG----RTLGNPLTTR-KVESIIIHENYAAHKHDDDIAVVKLSSPVLFSENLRTVCLPEATFQVLPKSKVFVTGWGALKANGPFPNSLQEVEIEIISNDVCNQV--NVYGGAISSGMICAGFLTGKL-DACEGDSGGPLVISDNRNKWYLLGIVSWGIDCGKENKPGIYTRVTHYRNWIK 412          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|74835253|sp|Q27081.1|CFB_TACTR (RecName: Full=Clotting factor B; AltName: Full=Coagulation factor B; Contains: RecName: Full=Clotting factor B light chain; Contains: RecName: Full=Clotting factor B heavy chain; Flags: Precursor)

HSP 1 Score: 157.532 bits (397), Expect = 1.963e-40
Identity = 111/336 (33.04%), Postives = 161/336 (47.92%), Query Frame = 0

Query:  603 VHAPIMTTPTTTTTTTTTTVWTTTTAPTTPSHSPVGQGE-PVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRS----GRQFCGGSLIDSTHILTAAHCVAQMSAQDV-QMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTR---YTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVS----PAGIKDHMMCAGKK--GKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            V  P    P   TTT  T +  +T  P  P     G G+  V     + G  G     PIS  +N      +          +  G  A    WPW+AA+       GR  C GS+I + +IL+AAH       +     L VR+G H I+R G+    E PV  V+ H  +     YND+A+I LK  +  + +D + P+CLP   T       R+ T  GWG L  +GP+  VL+E+++ +    +CDQ Y+ ++      GI ++ +CAG +  GKD+C GDSGGP M+ N  RW  VG+VS+G  C +  +PGVY+R      WI +  N  
Sbjct:   78 VCCPDAIAPPPVTTTAVTVI--STKEPKLPRLHISGCGKRKVKIDITTVGRSGSPILPPISTPQNSTGGRGI----------IAGGVEAKIGAWPWMAAVFVKNFGIGRFHCAGSIISNKYILSAAHAFLIGGRKLTPTRLAVRVGGHYIKR-GQ----EYPVKDVIIHPHYVEKENYNDIAIIELKEEL--NFTDLVNPICLPDPETVTDPLKDRIVTAAGWGDLDFSGPRSQVLREVSIPVVPVDKCDQAYEKLNTPSLKNGITNNFLCAGLEEGGKDACQGDSGGPLMLVNNTRWIVVGVVSFGHKCAEEGYPGVYSRVASYLDWIAKVTNSL 394          

HSP 2 Score: 137.117 bits (344), Expect = 1.520e-33
Identity = 91/253 (35.97%), Postives = 132/253 (52.17%), Query Frame = 0

Query:  217 IVNGTEAKQHEFPFMAALF-KN---SRQFCGGSLLDKTHILTAAHCVEHMSQNDV-RQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFN--YEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGN----QAPGGITTHMICAAGTF--RDSCSGDSGGPLFDCTD-RCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLD 455
            I  G EAK   +P+MAA+F KN    R  C GS++   +IL+AAH      +     ++ V +G H ++ RG    Q++ VK V+ H  +     +ND+A++ L E   FT  V PICL          + R V  AGWG +   G +S +LR+V + V   DKC ++Y          GIT + +CA      +D+C GDSGGPL    + R   +G+VS+G  CA   +PGVY+RV   + WI K+   LD
Sbjct:  148 IAGGVEAKIGAWPWMAAVFVKNFGIGRFHCAGSIISNKYILSAAHAFLIGGRKLTPTRLAVRVGGHYIK-RG----QEYPVKDVIIHPHYVEKENYNDIAIIELKEELNFTDLVNPICLPDPETVTDPLKDRIVTAAGWGDLDFSGPRSQVLREVSIPVVPVDKCDQAYEKLNTPSLKNGITNNFLCAGLEEGGKDACQGDSGGPLMLVNNTRWIVVGVVSFGHKCAEEGYPGVYSRVASYLDWIAKVTNSLD 395          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|136433|sp|P19799.1|TRY1_XENLA (RecName: Full=Trypsin; Flags: Precursor)

HSP 1 Score: 151.754 bits (382), Expect = 4.390e-40
Identity = 94/238 (39.50%), Postives = 135/238 (56.72%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQ-PDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIE 917
            +++ G    K+  P+I +L  SG  FCGGSLI +  +++AAHC           + VRLGEHNI     +  F +  ++V+RH G++S TL ND+ LI L +  P S++  +  V LPSG +   G    + GWG+   NG   PD+LQ L   I  NA+C+  Y    P  I  +M+C G  + GKDSC GDSGGP ++ NG   +  G+VSWG GC   ++PGVYT+  +   WI+
Sbjct:   20 KIIGGATCAKSSVPYIVSL-NSGYHFCGGSLITNQWVVSAAHCYKAS-------IQVRLGEHNIALSEGTEQFISS-SKVIRHSGYNSYTLDNDIMLIKLSS--PASLNAAVNTVPLPSGCSA-AGTSCLISGWGNTLSNGSNYPDLLQCLNAPILTNAQCNSAY----PGEITANMICVGYMEGGKDSCQGDSGGP-VVCNG---QLQGVVSWGYGCAMRNYPGVYTKVCNYNAWIQ 237          

HSP 2 Score: 127.102 bits (318), Expect = 1.211e-31
Identity = 79/239 (33.05%), Postives = 124/239 (51.88%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSA-ILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGT--FRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
            +I+ G    +   P++ +L  +   FCGGSL+    +++AAHC +         ++V LG+HN+    E  EQ      V+RH  + + TL ND+ ++ LS  A     V  + L      +  G S  ++GWG+   +GS    +L+ ++  + +N +C  +Y    PG IT +MIC       +DSC GDSGGP+  C  + +  G+VSWG GCA   +PGVYT+V     WIQ  +
Sbjct:   20 KIIGGATCAKSSVPYIVSL-NSGYHFCGGSLITNQWVVSAAHCYK-------ASIQVRLGEHNI-ALSEGTEQFISSSKVIRHSGYNSYTLDNDIMLIKLSSPASLNAAVNTVPLPSG--CSAAGTSCLISGWGNTLSNGSNYPDLLQCLNAPILTNAQCNSAY----PGEITANMICVGYMEGGKDSCQGDSGGPVV-CNGQLQ--GVVSWGYGCAMRNYPGVYTKVCNYNAWIQNTI 240          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|657341407|sp|B5U2W0.1|VSP_BOMIG (RecName: Full=Venom serine protease Bi-VSP; AltName: Full=Thrombin-like enzyme; Flags: Precursor)

HSP 1 Score: 154.451 bits (389), Expect = 8.548e-40
Identity = 91/243 (37.45%), Postives = 130/243 (53.50%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAAL-LRSGRQ-------FCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGE-SNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSG----STRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAG--KKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTT 910
            RVV G PA    WPWIAAL  R  R         CGGSLI S H+LTAAHC     A+  ++  VR+G+ N+ R  + ++  +  +   + H  + S    +D+A++ L   VP   S+ + P+CLP      +  +      V GWGSL  +GP  D L E+ + +  N  C   Y   + A + D ++CAG  + GKD+C GDSGGP M+     + Q+G+VS+G  C  A +PGVYTR T
Sbjct:  113 RVVGGKPAVLGAWPWIAALGFRYPRNPALEPLWKCGGSLISSRHVLTAAHC-----AEINELYVVRIGDLNLVRNDDGAHPVQIEIESKIIHPDYISGVTKHDIAILKLVEEVP--FSEYVYPICLPVEDNLRNNNFERYYPFVAGWGSLAHHGPGSDDLMEVQVPVISNTECKNSYARFAAAHVTDTVLCAGYTQGGKDACQGDSGGPLMLPKKFTFYQIGVVSYGHKCAAAGYPGVYTRVT 348          

HSP 2 Score: 151.369 bits (381), Expect = 9.522e-39
Identity = 118/362 (32.60%), Postives = 163/362 (45.03%), Query Frame = 0

Query:   98 LPQFQQPPVQAPLGNACVTNEGQNGTCTTVKECYPYFRLFNFDADETSTFGTYDTCSIDTRAGKKIFGICCVEKNIKADLLAEIEALRTEEPINPDEFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAAL-FKNSRQ-------FCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNL-RTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLE-QDGLFN--YEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTF--RDSCSGDSGGPLFDCTD-RCRQIGIVSWGIGCARPVFPGVYTRVTELM 444
            L  F  P V       C T   + G C  ++ C P   +        + F     C  +         +CC  +  + D    I      EP+ P           +CG  +V     R+V G  A    +P++AAL F+  R         CGGSL+   H+LTAAHC E      VR     +GD NL R    A   Q  +++ + H  + +    +D+A+L L E   F+  V PICL  +D L N  +E     VAGWGS+A  G  S  L +V V V SN +C+ SY   A   +T  ++CA  T   +D+C GDSGGPL         QIG+VS+G  CA   +PGVYTRVT  +
Sbjct:   13 LIAFLHPLVHVASAQECTTPNNKAGKCLGIRVCKPLLEMLQTQGHAAADFLRQSVCKYENNNPI----VCCPNEESRED--RGILVGNEYEPLRP----------PHCGFSNVSHT--RVVGGKPAVLGAWPWIAALGFRYPRNPALEPLWKCGGSLISSRHVLTAAHCAEINELYVVR-----IGDLNLVRNDDGAHPVQIEIESKIIHPDYISGVTKHDIAILKLVEEVPFSEYVYPICLPVEDNLRNNNFERYYPFVAGWGSLAHHGPGSDDLMEVQVPVISNTECKNSYARFAAAHVTDTVLCAGYTQGGKDACQGDSGGPLMLPKKFTFYQIGVVSYGHKCAAAGYPGVYTRVTSYL 351          

BLAST of trypsin-1 precursor vs. SwissProt
Match: gi|317373502|sp|Q86T26.3|TM11B_HUMAN (RecName: Full=Transmembrane protease serine 11B; AltName: Full=Airway trypsin-like protease 5)

HSP 1 Score: 153.295 bits (386), Expect = 6.455e-39
Identity = 92/238 (38.66%), Postives = 129/238 (54.20%), Query Frame = 0

Query:  683 RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVV-GWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKG--KDSCSGDSGGPFMIGNG-DRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWI 916
            ++VNG  + +  WPW A++   GR +CG SLI S  +L+AAHC A+    + +  TV  G   I       T    V  ++ H+ +SS  L++D+AL+ L   V  S ++ IR +CLP    + +     VV GWG+L  NG  P +LQE  ++I DN  C+  Y       + D M+CAG      D+C  DSGGP    +  + W  VGIVSWG GCGK + PGVYTR T  + WI
Sbjct:  184 KIVNGKSSLEGAWPWQASMQWKGRHYCGASLISSRWLLSAAHCFAK--KNNSKDWTVNFG---IVVNKPYMT--RKVQNIIFHENYSSPGLHDDIALVQLAEEV--SFTEYIRKICLPEAKMKLSENDNVVVTGWGTLYMNGSFPVILQEDFLKIIDNKICNASY--AYSGFVTDTMLCAGFMSGEADACQNDSGGPLAYPDSRNIWHLVGIVSWGDGCGKKNKPGVYTRVTSYRNWI 410          

HSP 2 Score: 137.887 bits (346), Expect = 1.272e-33
Identity = 84/257 (32.68%), Postives = 135/257 (52.53%), Query Frame = 0

Query:  201 VNENCGKRSVGSNLV---RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLG---DHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTF--RDSCSGDSGGPLF--DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWI 447
            +  NC  R V ++++   +IVNG  + +  +P+ A++    R +CG SL+    +L+AAHC     +N+ +   V  G   +    TR        +V+ ++ H+ + +  LH+D+A++ L+E   FT  ++ ICL +  +   E  +V V GWG++  +GS   IL++  +++  N  C  SY     G +T  M+CA       D+C  DSGGPL   D  +    +GIVSWG GC +   PGVYTRVT    WI
Sbjct:  166 LTNNCCGRQVANSIITGNKIVNGKSSLEGAWPWQASMQWKGRHYCGASLISSRWLLSAAHCFA--KKNNSKDWTVNFGIVVNKPYMTR--------KVQNIIFHENYSSPGLHDDIALVQLAEEVSFTEYIRKICLPEAKMKLSENDNVVVTGWGTLYMNGSFPVILQEDFLKIIDNKICNASYA--YSGFVTDTMLCAGFMSGEADACQNDSGGPLAYPDSRNIWHLVGIVSWGDGCGKKNKPGVYTRVTSYRNWI 410          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1022771057|gb|KZS15399.1| (Serine protease 42 [Daphnia magna])

HSP 1 Score: 408.297 bits (1048), Expect = 1.282e-122
Identity = 279/750 (37.20%), Postives = 390/750 (52.00%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFN-YEGRSVQVAGWGSIAEDGSQ-SAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF---DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILTPLDIKAGADTLVNSIGPGP--KSTPSHWAGGTP--VMGHCVTLKSCFP--LFNYQANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLY-PQYPGILGVPGAQGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVH------------------PGIPGVVQPPPPSYPVHAPIMTTPTTTTTTTTTTVWTTTTAP-------------TTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGST--RYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDR--WEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            RI+ GT + ++ +PF  +L  N R FCGGSLLD+ HILTAAHC+  +   ++ ++++ LG H L+       ++ ++K VVRHK + + + +ND+A+L +     F+ T+ P+CL   G  + Y  ++  V GWG++ E G      L++V V V +N +CR+SYG++APGGI  +M+CAA   +DSC GDSGGPL           Q+GIVSWGI     +   V  R  +    ++   T   +   +   V  +  G    S  + W   TP    G C +++SC P      Q +  S M   +  C  +EP  RQ + GVCC+ +Q   + +Y P + G  G P   GG  +P    P+ P    FG P F   G                      P        P  + P   P   T  + T+ TT+T   +TT+P              T S +     EPV +    +  CG G    +S  E        QR       R+V G  A +N WP+I AL   GR FCGGSLID++HILTAAHC+A MS+ D+  L V LG H ++   +S      V RVVRHKGF   TLYNDVA++ L +  P + S+ I PVCLP   T  ++  +  TVVGWG+LRE G QP+ LQ++T++I  NA C + Y   +P GI DHM+CA   G+DSCSGDSGGP +        W Q GIVSWGIGCGK  +PGVY+R T    WI +
Sbjct:  375 RIIGGTTSAKNAWPFAVSLMSNDRHFCGGSLLDEHHILTAAHCIAPLPTEELSEVEIILGLHTLKPLDPQIVRR-KLKRVVRHKGYHSDSFYNDIAILKMDSPVQFSDTISPVCLPSFGTTDLYVNKAAVVVGWGALQEGGKLLPKSLQQVTVRVQTNAECRKSYGSEAPGGILDNMLCAASPNKDSCMGDSGGPLVVQASPGSPWIQVGIVSWGI--VEDIKESVKGRSMDKSDVVKSQPTDEPVLGRSSKFVPMLPSGTFLLSDRTCW---TPDGKFGTCSSVRSCNPNVKLPLQKDVDSGMPSARGTCHYSEPDGRQ-VYGVCCAVKQAPSFGIYGPPFGGSFGFPANLGGPWWP----PYFP----FGGPSFPDVGTSPFIPPTTTTTRPSTTSPWWVPDPTTTTSRPGTTSPWWVPDQLTTKSPTSVTTSTNRPSTTSPWWVPESTTSTTSTPTTSTTSKPSVEPVVATDEGTRSCGRGPKKTLSLDE--------QR-------RIVGGTTALRNSWPFIVALFSRGRHFCGGSLIDASHILTAAHCIAHMSSTDISRLEVALGMHTLKPY-DSQALRKRVRRVVRHKGFDPVTLYNDVAILALDS--PVTFSNAISPVCLPPVGTTDQFVSKESTVVGWGALREGGRQPESLQQVTVQIQSNAECKRNYGKDAPGGIVDHMICAAYPGRDSCSGDSGGPMVTQEAPNSPWVQAGIVSWGIGCGKPPYPGVYSRVTSFLDWINK 1091          

HSP 2 Score: 182.185 bits (461), Expect = 2.557e-43
Identity = 118/298 (39.60%), Postives = 160/298 (53.69%), Query Frame = 0

Query:  612 TTTTTTTTTTVW------TTTTAPTTPSHSPVGQGEPVGSGSVSS---GECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTR--YTGRLGTVVGWGSLRENGP-QPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMI--GNGDRWEQVGIVSWGI 895
            TTT+  TTT+ W      +T   P   S  P  +   V   +VS    G CG G    I +                   R++ G  + KN WP+  +L+ + R FCGGSL+D  HILTAAHC+A +  +++  + + LG H + +  +       + RVVRHKG+ SD+ YND+A+  LK   P   SDTI PVCLPS  T   Y  +   VVGWG+L+E G   P  LQ++T+ +  NA C + Y   +P GI D+M+CA    KDSC GDSGGP ++    G  W QVGIVSWGI
Sbjct:  313 TTTSRPTTTSPWWFPDASSTLKLPVVTSTHPAIETSTVAMTTVSQSSGGSCGRGPFKLIEWP------------------RIIGGTTSAKNAWPFAVSLMSNDRHFCGGSLLDEHHILTAAHCIAPLPTEELSEVEIILGLHTL-KPLDPQIVRRKLKRVVRHKGYHSDSFYNDIAI--LKMDSPVQFSDTISPVCLPSFGTTDLYVNKAAVVVGWGALQEGGKLLPKSLQQVTVRVQTNAECRKSYGSEAPGGILDNMLCAASPNKDSCMGDSGGPLVVQASPGSPWIQVGIVSWGI 589          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1325331106|ref|XP_023347415.1| (trypsin-1-like isoform X3 [Eurytemora affinis])

HSP 1 Score: 364.385 bits (934), Expect = 1.579e-113
Identity = 185/374 (49.47%), Postives = 242/374 (64.71%), Query Frame = 0

Query:   98 LPQFQQPPVQAPLGNACVTNEGQNGTCTTVKECYPYFRLFNFDADETSTFGTYDTCSIDTRAGKKIFGICC-------VEKNIKADLLAEIEALRTEEPINPDEFPLDAQVNENCGKRS----------VGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCT--DRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKILT 452
            L     PP+Q P    C+  EG  G C ++ ECYPYF++FN    +T   G YDTCS  +  G ++FG+CC        E+N   D   E + +         EFP++  +  NCG R+           G+    IVNG EA  HE+PFMAAL   +RQFCGGSL+D  HILTAAHCV H+S+NDV+ ++V LGDHN+++   A   + RVK V+RH  F +STLHNDVA+LTL E   +TPT+QPICL   G  ++  + V VAGWG+++E G+Q A L KV+VEVWSN++C+ SYG+ APGGIT  M+CA+   +DSCSGDSGGPLF C    +C QIGIVSWGIGCA+  +PGVYTRVTE+  WI +I+T
Sbjct:   54 LSPLLTPPLQQP----CLNKEGNVGRCKSLNECYPYFKIFNLSWRDTWVMGHYDTCSYRSPEGSQVFGVCCDSTPRSVTEEN--EDDNDETKFIDENNDAIVGEFPIEFALAGNCGFRTPQPSVTLYDKTGAQFY-IVNGAEAVPHEYPFMAALMNRNRQFCGGSLIDANHILTAAHCVAHLSKNDVQHLRVRLGDHNIKSNSAAGRVEKRVKRVIRHIGFSSSTLHNDVAILTLEEDVEYTPTIQPICLAA-GTNSFVNQRVSVAGWGTLSEGGTQPASLNKVEVEVWSNERCKNSYGSSAPGGITKEMLCASLPEQDSCSGDSGGPLFSCPYGGQCTQIGIVSWGIGCAKAQYPGVYTRVTEMRDWISRIVT 419          

HSP 2 Score: 254.218 bits (648), Expect = 4.348e-72
Identity = 122/236 (51.69%), Postives = 160/236 (67.80%), Query Frame = 0

Query:  684 VVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGP-FMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            +VNG  A  +E+P++AAL+   RQFCGGSLID+ HILTAAHCVA +S  DVQ L VRLG+HNI+    +   E  V RV+RH GFSS TL+NDVA++TL+  V    + TI+P+CL +G+  +  +  +V GWG+L E G QP  L ++ +E+W N RC   Y   +P GI   M+CA    +DSCSGDSGGP F    G +  Q+GIVSWGIGC KA +PGVYTR T+++ WI R
Sbjct:  183 IVNGAEAVPHEYPFMAALMNRNRQFCGGSLIDANHILTAAHCVAHLSKNDVQHLRVRLGDHNIKSNSAAGRVEKRVKRVIRHIGFSSSTLHNDVAILTLEEDV--EYTPTIQPICLAAGTNSFVNQRVSVAGWGTLSEGGTQPASLNKVEVEVWSNERCKNSYGSSAPGGITKEMLCASLPEQDSCSGDSGGPLFSCPYGGQCTQIGIVSWGIGCAKAQYPGVYTRVTEMRDWISR 416          

BLAST of trypsin-1 precursor vs. nr
Match: gi|225718884|gb|ACO15288.1| (Trypsin-1 precursor [Caligus clemensi])

HSP 1 Score: 349.747 bits (896), Expect = 1.671e-107
Identity = 181/390 (46.41%), Postives = 238/390 (61.03%), Query Frame = 0

Query:   99 PQFQQPPVQAPLGNACVTNEGQNGTCTTVKECYPYFRLFNF-DADETSTFGTYDTCSIDTRAGKKIFGICCV-------------------------EKNIKADLLAEIEALRTEE------------PINPDEFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLFDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKI 450
            PQ  + PV      AC+T  G+ G CT++K CYPYF++    + D T + G YD+CS     G+++FG+CC                          E    A+   + E   +EE              +PDE          C  R   +NL +IVNG +A  +E+PFMAALF  +R FCGGS++D  HILTAAHCV HM+++DVR ++V+LG+H++++  E   ++ RV+ ++RHKRF  STLHNDVA+LTL E+  +   +QPICL  D    YEG +V VAGWG+I E G QS  L+KVDV VW N +C  SYGN+APGGI +HM+CA+   +DSCSGDSGGPLF C   C Q+GIVSWGIGCAR  FPGVYTRVT L  WI+KI
Sbjct:   68 PQGAKDPV---FEQACLTRTGRLGQCTSLKVCYPYFKMPGAPNQDSTPSLGLYDSCSYVGEQGQQVFGVCCTGEQRLPPGEPTSRPTQPPKPTPPGEEATKDANDGMDFELHESEEGSVTDKEEDNGIRTSPDETANPKFNTGYCSARFYNNNL-KIVNGEDASLNEYPFMAALFNRNRHFCGGSIIDPKHILTAAHCVAHMTKSDVRHLRVHLGEHDIKSNYETGVRKLRVQRIIRHKRFSASTLHNDVAILTLRESVSYFDAIQPICLATDNSVRYEGDAVTVAGWGTIGEGGRQSRTLQKVDVTVWRNFECAASYGNRAPGGIQSHMLCASRPGKDSCSGDSGGPLFICEGVCTQVGIVSWGIGCAREQFPGVYTRVTALYSWIEKI 453          

HSP 2 Score: 253.062 bits (645), Expect = 2.552e-71
Identity = 124/240 (51.67%), Postives = 160/240 (66.67%), Query Frame = 0

Query:  682 LRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPS-GSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERAR 920
            L++VNG  A  NE+P++AAL    R FCGGS+ID  HILTAAHCVA M+  DV+ L V LGEH+I+   E+   +  V R++RHK FS+ TL+NDVA++TL+ +V  S  D I+P+CL +  S RY G   TV GWG++ E G Q   LQ++ + +W N  C   Y   +P GI+ HM+CA + GKDSCSGDSGGP  I  G    QVGIVSWGIGC +  FPGVYTR T +  WIE+ R
Sbjct:  218 LKIVNGEDASLNEYPFMAALFNRNRHFCGGSIIDPKHILTAAHCVAHMTKSDVRHLRVHLGEHDIKSNYETGVRKLRVQRIIRHKRFSASTLHNDVAILTLRESV--SYFDAIQPICLATDNSVRYEGDAVTVAGWGTIGEGGRQSRTLQKVDVTVWRNFECAASYGNRAPGGIQSHMLCASRPGKDSCSGDSGGPLFICEG-VCTQVGIVSWGIGCAREQFPGVYTRVTALYSWIEKIR 454          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1325331102|ref|XP_023347412.1| (transmembrane protease serine 9-like isoform X1 [Eurytemora affinis])

HSP 1 Score: 336.265 bits (861), Expect = 1.111e-101
Identity = 158/272 (58.09%), Postives = 198/272 (72.79%), Query Frame = 0

Query:  653 CGVGRHFPISYGENGEDVEAVQRIEGEHPL-RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            CGVG++  I Y +NG   E V+R+       ++VNG  A+ NEWPWIAAL+ +GRQFCGGSLID  HILTAAHCVA MS  DV  L +RLGEH I+  G++  FE+ V+RVVRHK FS  TL+ DVA++T+ + VP + +   RPVCLP+G   Y  R  TVVGWGSLRENGPQPD+LQE+T +IW+NA C + Y   +P GI+ HM+CAGK+G DSCSGDSGGP  IG G  W Q+GIVSWGIGCGK+ +PGVYTR T+V+ WI+R    +
Sbjct:  239 CGVGKYPVIRYAQNGSVAEEVERVTCNGSCSKIVNGNDAEDNEWPWIAALMNNGRQFCGGSLIDDKHILTAAHCVAHMSRYDVANLKIRLGEHKIKSGGDT-IFESKVSRVVRHKDFSQQTLHKDVAILTMADPVPSTFTRA-RPVCLPTGHELYADRTATVVGWGSLRENGPQPDILQEMTAKIWNNAECKETYGNAAPGGIEPHMLCAGKQGLDSCSGDSGGPMQIGEGQHWTQIGIVSWGIGCGKSFYPGVYTRVTEVRDWIDRIVKSY 508          

HSP 2 Score: 247.284 bits (630), Expect = 1.561e-68
Identity = 122/260 (46.92%), Postives = 170/260 (65.38%), Query Frame = 0

Query:  194 EFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPT-VQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPL-FDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
             +  +  V E   + +   +  +IVNG +A+ +E+P++AAL  N RQFCGGSL+D  HILTAAHCV HMS+ DV  +K+ LG+H +++ G+   +  +V  VVRHK F   TLH DVA+LT+++    T T  +P+CL   G   Y  R+  V GWGS+ E+G Q  IL+++  ++W+N +C+ +YGN APGGI  HM+CA     DSCSGDSGGP+         QIGIVSWGIGC +  +PGVYTRVTE+  WI +I+
Sbjct:  248 RYAQNGSVAEEVERVTCNGSCSKIVNGNDAEDNEWPWIAALMNNGRQFCGGSLIDDKHILTAAHCVAHMSRYDVANLKIRLGEHKIKSGGDTIFES-KVSRVVRHKDFSQQTLHKDVAILTMADPVPSTFTRARPVCLP-TGHELYADRTATVVGWGSLRENGPQPDILQEMTAKIWNNAECKETYGNAAPGGIEPHMLCAGKQGLDSCSGDSGGPMQIGEGQHWTQIGIVSWGIGCGKSFYPGVYTRVTEVRDWIDRIV 505          

BLAST of trypsin-1 precursor vs. nr
Match: gi|1325331104|ref|XP_023347414.1| (transmembrane protease serine 9-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 335.88 bits (860), Expect = 1.236e-101
Identity = 158/272 (58.09%), Postives = 198/272 (72.79%), Query Frame = 0

Query:  653 CGVGRHFPISYGENGEDVEAVQRIEGEHPL-RVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIERARNKF 923
            CGVG++  I Y +NG   E V+R+       ++VNG  A+ NEWPWIAAL+ +GRQFCGGSLID  HILTAAHCVA MS  DV  L +RLGEH I+  G++  FE+ V+RVVRHK FS  TL+ DVA++T+ + VP + +   RPVCLP+G   Y  R  TVVGWGSLRENGPQPD+LQE+T +IW+NA C + Y   +P GI+ HM+CAGK+G DSCSGDSGGP  IG G  W Q+GIVSWGIGCGK+ +PGVYTR T+V+ WI+R    +
Sbjct:  238 CGVGKYPVIRYAQNGSVAEEVERVTCNGSCSKIVNGNDAEDNEWPWIAALMNNGRQFCGGSLIDDKHILTAAHCVAHMSRYDVANLKIRLGEHKIKSGGDT-IFESKVSRVVRHKDFSQQTLHKDVAILTMADPVPSTFTRA-RPVCLPTGHELYADRTATVVGWGSLRENGPQPDILQEMTAKIWNNAECKETYGNAAPGGIEPHMLCAGKQGLDSCSGDSGGPMQIGEGQHWTQIGIVSWGIGCGKSFYPGVYTRVTEVRDWIDRIVKSY 507          

HSP 2 Score: 246.899 bits (629), Expect = 1.602e-68
Identity = 122/260 (46.92%), Postives = 170/260 (65.38%), Query Frame = 0

Query:  194 EFPLDAQVNENCGKRSVGSNLVRIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPT-VQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPL-FDCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQKIL 451
             +  +  V E   + +   +  +IVNG +A+ +E+P++AAL  N RQFCGGSL+D  HILTAAHCV HMS+ DV  +K+ LG+H +++ G+   +  +V  VVRHK F   TLH DVA+LT+++    T T  +P+CL   G   Y  R+  V GWGS+ E+G Q  IL+++  ++W+N +C+ +YGN APGGI  HM+CA     DSCSGDSGGP+         QIGIVSWGIGC +  +PGVYTRVTE+  WI +I+
Sbjct:  247 RYAQNGSVAEEVERVTCNGSCSKIVNGNDAEDNEWPWIAALMNNGRQFCGGSLIDDKHILTAAHCVAHMSRYDVANLKIRLGEHKIKSGGDTIFES-KVSRVVRHKDFSQQTLHKDVAILTMADPVPSTFTRARPVCLP-TGHELYADRTATVVGWGSLRENGPQPDILQEMTAKIWNNAECKETYGNAAPGGIEPHMLCAGKQGLDSCSGDSGGPMQIGEGQHWTQIGIVSWGIGCGKSFYPGVYTRVTEVRDWIDRIV 504          

BLAST of trypsin-1 precursor vs. nr
Match: gi|909560120|ref|XP_013135138.1| (PREDICTED: proclotting enzyme-like isoform X1 [Papilio polytes])

HSP 1 Score: 335.495 bits (859), Expect = 4.943e-101
Identity = 203/470 (43.19%), Postives = 258/470 (54.89%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIP-FSPGQGG----FGTPG-----------FGGSG------IGVHPGIPGV-VQPPPPSYPVHAPIM-------TTPTTTTT-TTTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C   + +    + GVCC+                +G P  Q   +   G +P   P Q       G PG           FG +       I   P IP +   PP  + P H P +       T PTT+   +T +T W T    TT  T P   P    +P G  +V S  CG+ ++ P +YG   E       ++ E   R+V G  AD NEWPWIAAL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF+  TLYNDVA++TL    P   S  IRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW NA C   Y   +P GI +HM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   91 GTCKSFRDCYPLFKVVDLSGWDGWVMGHYDTCSFID-SNNMEVFGVCCT--------------APVGTPPQQEPDVQRLGALPPVLPAQAPPTQLVGFPGRIATSMSQQWTFGEANRERQIPINWPPTIPPLPTHPPDHTAPTHPPSIVAGNVEPTKPTTSRPPSTASTTWGTKPPSTTKQTWPPAYPTQPTKPAGQPAVDS-SCGI-KNGPQTYGTAYE-------LQDEE--RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDK--PVKFSKAIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNAECRLKYGSAAPGGIVEHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 532          

HSP 2 Score: 261.151 bits (666), Expect = 2.228e-73
Identity = 123/235 (52.34%), Postives = 160/235 (68.09%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH+ F   TL+NDVA+LTL +   F+  ++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  299 RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDKPVKFSKAIRPICLP-SGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNAECRLKYGSAAPGGIVEHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 532          

BLAST of trypsin-1 precursor vs. nr
Match: gi|910346044|ref|XP_013176913.1| (PREDICTED: proclotting enzyme-like isoform X2 [Papilio xuthus])

HSP 1 Score: 333.183 bits (853), Expect = 3.694e-100
Identity = 201/468 (42.95%), Postives = 250/468 (53.42%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIP-----FSPGQGGFGTPGFGGSGI---------GVHPGIPGVVQPPPPSYPVHAPIMTTPT----------TTTTT-----TTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C     +    + GVCC+                +G P  Q   +   G +P      +P       PG   S +              IP    P  P  P H P  T PT          T  TT     T +T W T    TT  T P   P    +P G  +V S  CG+ ++ P +YG   E       ++ E   R+V G  AD NEWPWIAAL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF+  TLYNDVA++TL    P   S TIRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW N  C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   93 GTCKSFRDCYPLFKVVDLSGWDGWVMGHYDTCSFIN-SNNMEVFGVCCT--------------APVGTPPQQEPDVQRLGALPPVLPAQAPPTQFLSFPGRIASSLTQQWTFGEANRERQIPANWPPTIPPLPTHPPDHTAPTHPPSIGNVEPTRPTTSRPPSTASTTWGTKPPSTTKQTWPPAYPTQPTKPTGQPAVDS-SCGI-KNGPQTYGTAYE-------LQDEE--RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDK--PVKFSKTIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 532          

HSP 2 Score: 262.307 bits (669), Expect = 7.982e-74
Identity = 124/235 (52.77%), Postives = 161/235 (68.51%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH+ F   TL+NDVA+LTL +   F+ T++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  299 RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDKPVKFSKTIRPICLP-SGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 532          

BLAST of trypsin-1 precursor vs. nr
Match: gi|910346042|ref|XP_013176912.1| (PREDICTED: proclotting enzyme-like isoform X1 [Papilio xuthus])

HSP 1 Score: 332.798 bits (852), Expect = 5.084e-100
Identity = 201/470 (42.77%), Postives = 250/470 (53.19%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIP-----FSPGQGGFGTPGFGGSGI---------GVHPGIPGVVQPPPPSYPVHAPIMTTPT------------TTTTT-----TTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C     +    + GVCC+                +G P  Q   +   G +P      +P       PG   S +              IP    P  P  P H P  T PT            T  TT     T +T W T    TT  T P   P    +P G  +V S  CG+ ++ P +YG   E       ++ E   R+V G  AD NEWPWIAAL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF+  TLYNDVA++TL    P   S TIRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW N  C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   93 GTCKSFRDCYPLFKVVDLSGWDGWVMGHYDTCSFIN-SNNMEVFGVCCT--------------APVGTPPQQEPDVQRLGALPPVLPAQAPPTQFLSFPGRIASSLTQQWTFGEANRERQIPANWPPTIPPLPTHPPDHTAPTHPPSIVAGNVEPTRPTTSRPPSTASTTWGTKPPSTTKQTWPPAYPTQPTKPTGQPAVDS-SCGI-KNGPQTYGTAYE-------LQDEE--RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDK--PVKFSKTIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 534          

HSP 2 Score: 262.692 bits (670), Expect = 7.978e-74
Identity = 124/235 (52.77%), Postives = 161/235 (68.51%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH+ F   TL+NDVA+LTL +   F+ T++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  301 RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDKPVKFSKTIRPICLP-SGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 534          

BLAST of trypsin-1 precursor vs. nr
Match: gi|930652485|gb|KPI94824.1| (Proclotting enzyme [Papilio xuthus])

HSP 1 Score: 332.028 bits (850), Expect = 5.337e-100
Identity = 201/470 (42.77%), Postives = 250/470 (53.19%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCSTRQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIP-----FSPGQGGFGTPGFGGSGI---------GVHPGIPGVVQPPPPSYPVHAPIMTTPT------------TTTTT-----TTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C     +    + GVCC+                +G P  Q   +   G +P      +P       PG   S +              IP    P  P  P H P  T PT            T  TT     T +T W T    TT  T P   P    +P G  +V S  CG+ ++ P +YG   E       ++ E   R+V G  AD NEWPWIAAL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH+GF+  TLYNDVA++TL    P   S TIRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW N  C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   77 GTCKSFRDCYPLFKVVDLSGWDGWVMGHYDTCSFIN-SNNMEVFGVCCT--------------APVGTPPQQEPDVQRLGALPPVLPAQAPPTQFLSFPGRIASSLTQQWTFGEANRERQIPANWPPTIPPLPTHPPDHTAPTHPPSIVAGNVEPTRPTTSRPPSTASTTWGTKPPSTTKQTWPPAYPTQPTKPTGQPAVDS-SCGI-KNGPQTYGTAYE-------LQDEE--RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDK--PVKFSKTIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 518          

HSP 2 Score: 262.692 bits (670), Expect = 5.370e-74
Identity = 124/235 (52.77%), Postives = 161/235 (68.51%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++AALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH+ F   TL+NDVA+LTL +   F+ T++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  285 RIVGGHNADLNEWPWIAALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHRGFNMQTLYNDVAILTLDKPVKFSKTIRPICLP-SGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNGECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 518          

BLAST of trypsin-1 precursor vs. nr
Match: gi|943945785|ref|XP_014356121.1| (PREDICTED: LOW QUALITY PROTEIN: proclotting enzyme-like [Papilio machaon])

HSP 1 Score: 327.791 bits (839), Expect = 3.203e-98
Identity = 197/466 (42.27%), Postives = 249/466 (53.43%), Query Frame = 0

Query:  486 GHCVTLKSCFPLFNY--QANTPSWMMGEKDMCQTTEPTTRQSMAGVCCST------------------RQFGQYPLYPQYPGILGVPGAQGGGIYPAGTIPFSPGQGGFGTPGFGGSGIGVHPGIPGV-VQPPPPSYPVHAPIM-------TTPTTTT-TTTTTTVWTT----TTAPTTPSHSPVGQGEPVGSGSVSSGECGVGRHFPISYGENGEDVEAVQRIEGEHPLRVVNGWPADKNEWPWIAALLRSGRQFCGGSLIDSTHILTAAHCVAQMSAQDVQMLTVRLGEHNIRREGESNTFETPVTRVVRHKGFSSDTLYNDVALITLKNAVPESMSDTIRPVCLPSGSTRYTGRLGTVVGWGSLRENGPQPDVLQELTMEIWDNARCDQIYQGVSPAGIKDHMMCAGKKGKDSCSGDSGGPFMIGNGDRWEQVGIVSWGIGCGKADFPGVYTRTTDVKGWIER 918
            G C + + C+PLF     +    W+MG  D C     +    + GVCC+                    ++    L   +PG +     Q      A      P                  P IP +   PP  + P H P +       T PTT+  ++T +T W T    TT  T     P    +P G  +V S  CG+ ++ P +YG   E       I+ E   R+V G  AD NEWPWI AL   GRQFCGGSLID  HIL+AAHCVA M++ DV  LT RLG+HNIR   E+   E  + RVVRH GF+  TLYNDVA++TL    P   S TIRP+CLPSG+  Y G++ TV+GWGSLRE+GPQP VLQE+++ IW NA C   Y   +P GI DHM+CAGK   DSCSGDSGGP M+  G RW QVG+VSWGIGCGK  +PGVYTR T    WI++
Sbjct:   93 GTCKSFRDCYPLFKVVDLSGWDGWVMGHYDTCSFIN-SNNMEVFGVCCTAPVGTPSSTNFCKVNXIVKXKYLSKMLXCSFPGRIASSLTQQWTFGEANRERQIPANW--------------PPTIPPLPTHPPDHAAPTHPPSIVAGNVEPTRPTTSKPSSTASTTWGTKPPSTTKQTWSPAYPTKPTKPTGQPAVDS-SCGI-KNGPQTYGTAYE-------IQDEE--RIVGGHNADLNEWPWIVALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHHGFNMQTLYNDVAILTLDK--PVKFSKTIRPICLPSGNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNAECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 530          

HSP 2 Score: 259.61 bits (662), Expect = 9.333e-73
Identity = 123/235 (52.34%), Postives = 159/235 (67.66%), Query Frame = 0

Query:  216 RIVNGTEAKQHEFPFMAALFKNSRQFCGGSLLDKTHILTAAHCVEHMSQNDVRQMKVYLGDHNLRTRGEAAEQQFRVKTVVRHKRFKTSTLHNDVAVLTLSEAAVFTPTVQPICLEQDGLFNYEGRSVQVAGWGSIAEDGSQSAILRKVDVEVWSNDKCRRSYGNQAPGGITTHMICAAGTFRDSCSGDSGGPLF-DCTDRCRQIGIVSWGIGCARPVFPGVYTRVTELMPWIQK 449
            RIV G  A  +E+P++ ALF   RQFCGGSL+D  HIL+AAHCV HM+  DV ++   LGDHN+RT  E   ++ ++K VVRH  F   TL+NDVA+LTL +   F+ T++PICL   G   Y G+   V GWGS+ E G Q ++L++V + +W N +CR  YG+ APGGI  HMICA     DSCSGDSGGPL  +   R  Q+G+VSWGIGC +  +PGVYTRVT  +PWIQK
Sbjct:  297 RIVGGHNADLNEWPWIVALFNGGRQFCGGSLIDDIHILSAAHCVAHMTSWDVARLTARLGDHNIRTNTETQHEERKIKRVVRHHGFNMQTLYNDVAILTLDKPVKFSKTIRPICLPS-GNRAYAGQVATVIGWGSLRESGPQPSVLQEVSIPIWQNAECRLKYGSAAPGGIVDHMICAGKASMDSCSGDSGGPLMVNEGGRWTQVGVVSWGIGCGKGQYPGVYTRVTAFLPWIQK 530