BLAST of aconitase vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 1363.59 bits (3528), Expect = 0.000e+0
Identity = 656/895 (73.30%), Postives = 768/895 (85.81%), Query Frame = 0

Query:   24 SPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVL-NGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVS----GKKSFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            +PF  +L++ ++ G    K++D++SL P Y +LP+SIR+LLESAVRNCDNFQV E DV  IL W  N+      E+PFRP RVILQDFTGVPA+VDFAAMRDAV++LGGDP  INP CP+DLVIDHS+QVDF R   AL KNE+LEF+RNKERFTFLKWGA +F+NMLI+PPGSGIVHQVNLEYLARVCFTD+D+V+PDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLP+V+GY+ISG    LV STD+VLTITKHLRQ+GVVG+FVEFYGPG+  LSIADRATI+NMCPEYGAT+G FPVD  SI+YL+ TNR+++K+K I+ YL+   ++R+Y D S DPTFS++V LDL +VV S SGPKRP DRV+V+DMK DFL CLS  IGFKG+ +P +K   TVP +  + KE+++ HG+VLIAAITSCTNTSNPSVMLGAGL+AKKAV+KGL+V PYIKTSLSPGSGVVTYYLK SGVIPYLDALGFSIVGYG MTCIGNSGPLE+ V     KGDLVC GVLSGNRNFEGRI+  TRANYLASPPLVIAYAIAGRVDIDFE EALG DA+ +P++LRDIWP+R+EIQ VEKKY++P+MFE+VYSK+TTGN+RWN LEAP+G LYPWD  STYIKSPPFFD M++ELP P+K++NAL LLNL  S T DHISPAG IARN PAAR+L SK I+PREFNSYGSRRGNDA+MARGTFANIRLVNKF+ G+ GPKT+H+ S ++MDVFDAA EYKKEN+HL++LAG+DYGCGSSRDWAAKGPFL GVRAV+AESYERIHRSNLVGMGIVPLQY  GE+++SLG + KE+F+V +P +L PQQ L+++V       KSFK   RFDTELELTYYRHGGILNYMIRK+I+
Sbjct:    3 NPFDKILKNFEIGGK-TYKYYDVSSLDPSYHKLPYSIRILLESAVRNCDNFQVYEKDVETILNWEGNRG--KNAEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTDLVFPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAVTDMKNDFLQCLSNKIGFKGFAIPDNKKNITVPIIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRINPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDANNEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTTGNERWNSLEAPKGILYPWDIESTYIKSPPFFDNMSEELPEPKKIKNALALLNLGXSFTXDHISPAGXIARNXPAARYLASKNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDGSIKSFKATVRFDTELELTYYRHGGILNYMIRKVIS 894          

BLAST of aconitase vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 869.381 bits (2245), Expect = 0.000e+0
Identity = 452/919 (49.18%), Postives = 623/919 (67.79%), Query Frame = 0

Query:   29 LLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQ--VLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFV--------------RTP-----------------DALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGP-LEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDAD-GKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEG-KLYPWDQNSTYIKSPPFFDGMTKELPA-PRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQV-SGKKSFKVLARFDTELELTYYRHGGILNYMIRK 909
            LL+D +V G  + K++D+  +   Y +LP+S+RVLLE AVR     Q  V +    +IL+                  RV+LQDFTGVPA++D AA+R++V + GG P++++  CP+DLV+DH VQVD+               +TP                 DAL  N+  EFERNKERF FLKW   AFKN+ ++PPG+G++HQVNLEYLARV      +++PDSVVGTD+HTTMINGLGV+GWGVG ++A+AVM G P+++  P+V+G +I G +    TSTD+VL ITK LRQ GV G FVEF+GP +  LS+ADRATI+NMCPEYGA VG+FP+D  +I YL Q+ RD + I+ +E Y+  V M R+ SD +++  F+ ++E+DL+ ++P +SGPKR  D+VS++++  DF + L    G KGYGL + +        ++G+   + HG VL+AAI+SCTNTSNPSVMLGAGL+AKKAVE GLSV   +KTSL+PGSGVVT YL+ESGV+PYL  LGF IVGYGC TC  NSGP L + + +AIK+G+LVCCG+LSGNRNFEGR+  D RANYLASP +VIAYAIAGR+DI+ + E LG ++  G+ +FL++IWPSR+EIQ +E+K VIP ++ +VYSK++ GN  W+ L  P     +PW  +STYIK P +     KE+ +  R  +N   LL L D++T+DHISPAGSI R+SPAA +L S G+ PRE+NSYG+RRGN  VM RGTF+N+RL N  + + GP TLH+PS     +F+A+E YK +   L  + G+++G G+SRDWA +GPFL GVRA++A S++  +RSNLV  GI+PLQ  S        L   E  ++ I  ++ P+  ++V V   ++SFK + R D + E   +  GG++  ++ K
Sbjct:   15 LLKDFQV-GERSYKYYDVTQI-QGYEELPYSLRVLLECAVRKRSETQGQVWKDVYERILR------------------RVLLQDFTGVPALIDLAAIRESVSKSGGCPDVVDSLCPADLVVDHCVQVDYNQIKQQLSSPPDPSPKTPVLGDDICPFHQKMSYWADALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVVTCQGGVLFPDSVVGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRS-SDENENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYGLSQGQGHVAFSVNIDGEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTCSYNSGPNLPDSIVQAIKQGNLVCCGLLSGNRNFEGRLDPDIRANYLASPLMVIAYAIAGRIDINLDREPLGFNSHTGEAIFLKNIWPSRQEIQEIERKLVIPGIYRQVYSKVSVGNKHWDSLSVPNSVDKFPWSSDSTYIKCPEYI----KEIGSNARNWKNMRCLLKLGDNITSDHISPAGSITRSSPAAEYLSSLGLAPREYNSYGARRGNYHVMVRGTFSNMRLQNHLLNKVGPYTLHLPSEVTTTIFEASERYKNDGVSLFAIGGKNFGRGTSRDWATRGPFLLGVRAILAVSFDPTYRSNLVKTGILPLQITS---ISYESLKGTECLSIDIRDNITPKCLIDVVVGKAERSFKAVVRVDNDYETDVFHAGGVIAQIVSK 905          

BLAST of aconitase vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 140.584 bits (353), Expect = 4.408e-34
Identity = 151/522 (28.93%), Postives = 230/522 (44.06%), Query Frame = 0

Query:   89 QNQSVPSGVE-VPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFL-----KWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSV-VGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDP----TFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRAN-YLASPPLVIAYAIAGRVDIDFETEAL 598
            +NQ +  G   +  RP RV +QD T   A++ F +         G P++     PS +  DH +Q       D L++ ++L    N E + FL     K+G   +K      PGSGI+HQ+ LE  A          +P  + VGTDSHT    GLG +  GVGG +A  VM   P  +  PKV+G K++GK+    +  DV+L +   L   G  G  VE++G GV S+S     TI NM  E GAT   FP + +   YL  TNR            +   +   Y +    P     + +VVE+DL ++ P ++GP  P                L+  IG  G    K           NG    +  G      I SCTN+S   +     ++ K+A+ KG+         ++PGS  +   ++  G+   +   G  ++   C  CIG          + I KG+     V S NRNF GR  A+   + ++ SP + +A ++AG +D D  ++A+
Sbjct:  661 KNQDIVRGESYLRLRPDRVAMQDATAQMAMLQFIS--------SGLPKV---AVPSTIHCDHLIQAQIEGKKD-LARAKDL----NAEVYNFLSTSGAKYGVGFWK------PGSGIIHQIILENYA----------FPGLLMVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNRS-----------DIANLADKYREVLLSPDGGCKYDQVVEIDLNTLEPHVNGPFTPD---------------LATPIGELGEKAKK-----------NGWPLEVKDG-----LIGSCTNSSYEDMARXTAII-KQALAKGMKSKSNF--YITPGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWD------RQNITKGEKNTI-VTSYNRNFTGRNDANPATHGFVTSPEMTVALSLAGSLDFDPRSDAI 1098          

BLAST of aconitase vs. SwissProt
Match: gi|75225211|sp|Q6YZX6.1|ACOC_ORYSJ (RecName: Full=Putative aconitate hydratase, cytoplasmic; Short=Aconitase; AltName: Full=Citrate hydro-lyase)

HSP 1 Score: 1130.93 bits (2924), Expect = 0.000e+0
Identity = 550/896 (61.38%), Postives = 677/896 (75.56%), Query Frame = 0

Query:   21 SAPSPFGHLLRDLKVEGHGNL-KFFDLASLG-PEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIP---ADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKM 910
            +A  PF ++L  L   G G   KF+ L +L  P   +LP+SIR+LLESA+RNCDNFQV + DV KI+ W +N S P   E+PF+PARV+LQDFTGVPAVVD AAMRDA+ +LG D   INP  P DLVIDHSVQVD  R+P+A+  N ELEF+RN ERF FLKWG+ AF NML+VPPGSGIVHQVNLEYL RV F    I+YPDSVVGTDSHTTMI+GLGV GWGVGGIEAEA MLGQP+SMVLP V+G+K++GK+ + VT+TD+VLT+T+ LR+ GVVGKFVEFYG G+  LS+ADRATIANM PEYGAT+G+FPVD  ++DYLK T R    +  IEAYL A  M  +Y++   +  +S  +ELDL  V P +SGPKRPHDRV + +MK+D+ +CL   +GFKG+ +PK++    V F  +G+   + HG+V+IAAITSCTNTSNPSVMLGA L+AKKA E GL V P++KTSL+PGSGVVT YL +SG+  YL+  GF +VGYGC TCIGNSG L+E VS AI + D+V   VLSGNRNFEGR+H  TRANYLASPPLV+AYA+AG VDIDFE E +G   DGK VF RDIWPS EEI  V +  V+P MF+  Y  IT GN  WN+L  PE  LY WD NSTYI  PP+F  MT   P P  V+NA  LLN  DS+TTDHISPAGSI ++SPAA++L  +G+  ++FNSYGSRRGND VMARGTFANIR+VNKF+ G+ GPKT+H+P+ E + VFDAA +YK E    ++LAG +YG GSSRDWAAKGP L GV+AV+A+S+ERIHRSNLVGMGI+PL + +GE+ADSLGLT  E +T+ +P   ++++P Q + V     KSF    RFDTE+EL Y+ HGGIL Y+IR +
Sbjct:    2 AAEHPFKNILTTLPKPGGGEYGKFYSLPALNDPRIDKLPYSIRILLESAIRNCDNFQVNQNDVEKIIDW-ENTS-PKLAEIPFKPARVLLQDFTGVPAVVDLAAMRDAMAKLGSDANKINPLVPVDLVIDHSVQVDVARSPNAVQSNMELEFKRNNERFGFLKWGSTAFHNMLVVPPGSGIVHQVNLEYLGRVVFNTDGIMYPDSVVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLTGKLQNGVTATDLVLTVTQMLRKHGVVGKFVEFYGEGMGKLSLADRATIANMSPEYGATMGFFPVDHVTLDYLKLTGRSDETVAMIEAYLRANKMFVDYNEPQTERVYSSYLELDLNEVEPCISGPKRPHDRVLLKEMKSDWHSCLDNRVGFKGFAVPKEQQDKVVKFDFHGQPAELKHGSVVIAAITSCTNTSNPSVMLGAALVAKKACELGLEVKPWVKTSLAPGSGVVTKYLLQSGLQEYLNKQGFHVVGYGCTTCIGNSGDLDESVSAAISENDVVAAAVLSGNRNFEGRVHPLTRANYLASPPLVVAYALAGTVDIDFEKEPIGVGKDGKEVFFRDIWPSTEEIAEVVQSSVLPDMFKSTYEAITKGNPMWNQLTVPEASLYSWDPNSTYIHEPPYFKDMTMSPPGPHGVKNAYCLLNFGDSITTDHISPAGSIHKDSPAAKYLLERGVDRKDFNSYGSRRGNDEVMARGTFANIRIVNKFLNGEVGPKTVHVPTGEKLYVFDAALKYKSEGHDTIVLAGAEYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGHERYTIDLPTNVSEIRPGQDITVTTDNGKSFTCTLRFDTEVELAYFNHGGILPYVIRNL 895          

BLAST of aconitase vs. SwissProt
Match: gi|13124706|sp|Q42560.2|ACO1_ARATH (RecName: Full=Aconitate hydratase 1; Short=Aconitase 1; AltName: Full=Citrate hydro-lyase 1)

HSP 1 Score: 1118.99 bits (2893), Expect = 0.000e+0
Identity = 541/897 (60.31%), Postives = 674/897 (75.14%), Query Frame = 0

Query:   21 SAPSPFGHLLRDLKVEGHGNL-KFFDLASLG-PEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIP---ADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            ++ +PF  +L+ L+    G    ++ L +L  P   +LP+SIR+LLESA+RNCD FQV   DV KIL W +N S P  VE+PF+PARV+LQDFTGVPAVVD A MRDA+  LGGD   INP  P DLVIDHSVQVD  R+ +A+  N ELEF+RNKERF FLKWG+ AF NML+VPPGSGIVHQVNLEYLARV F  + ++YPDSVVGTDSHTTMI+GLGV GWGVGGIEAEA MLGQP+SMVLP V+G+K++GK+   +T+TD+VLT+T+ LR+ GVVGKFVEF+G G+  LS+ADRATIANM PEYGAT+G+FPVD  ++ YL+ T R    +  IEAYL A  M  +YS+      +S  +EL+L  V P +SGPKRPHDRV + +MK D+ +CL   +GFKG+ +PK+  +  V F  NG    + HG V+IAAITSCTNTSNPSVMLGA L+AKKA + GL V P+IKTSL+PGSGVVT YL +SG+  YL+ LGFSIVGYGC TCIGNSG + E V+ AI   DLV   VLSGNRNFEGR+H  TRANYLASPPLV+AYA+AG VDIDFET+ +G   DGK +F RDIWPS +E+  V +  V+P MF+  Y  IT GN  WN+L    G LY WD  STYI  PP+F GMT   P P  V++A  LLN  DS+TTDHISPAGSI ++SPAA++L  +G+  R+FNSYGSRRGND +MARGTFANIR+VNK + G+ GPKT+HIP+ E + VFDAA +Y+ E R  +ILAG +YG GSSRDWAAKGP L GV+AV+++S+ERIHRSNLVGMGI+PL + +GE+A++LGLT +E +T+ +P   +++KP Q + V  +  KSF    RFDTE+EL Y+ HGGIL Y+IR +I
Sbjct:    2 ASENPFRSILKALEKPDGGEFGNYYSLPALNDPRIDKLPYSIRILLESAIRNCDEFQVKSKDVEKILDW-ENTS-PKQVEIPFKPARVLLQDFTGVPAVVDLACMRDAMNNLGGDSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSNAFHNMLVVPPGSGIVHQVNLEYLARVVFNTNGLLYPDSVVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLTGKLRDGMTATDLVLTVTQMLRKHGVVGKFVEFHGEGMRELSLADRATIANMSPEYGATMGFFPVDHVTLQYLRLTGRSDDTVSMIEAYLRANKMFVDYSEPESKTVYSSCLELNLEDVEPCVSGPKRPHDRVPLKEMKADWHSCLDNRVGFKGFAVPKEAQSKAVEFNFNGTTAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACDLGLEVKPWIKTSLAPGSGVVTKYLAKSGLQKYLNQLGFSIVGYGCTTCIGNSGDIHEAVASAIVDNDLVASAVLSGNRNFEGRVHPLTRANYLASPPLVVAYALAGTVDIDFETQPIGTGKDGKQIFFRDIWPSNKEVAEVVQSSVLPDMFKATYEAITKGNSMWNQLSVASGTLYEWDPKSTYIHEPPYFKGMTMSPPGPHGVKDAYCLLNFGDSITTDHISPAGSIHKDSPAAKYLMERGVDRRDFNSYGSRRGNDEIMARGTFANIRIVNKHLKGEVGPKTVHIPTGEKLSVFDAAMKYRNEGRDTIILAGAEYGSGSSRDWAAKGPMLLGVKAVISKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGQELYTIELPNNVSEIKPGQDVTVVTNNGKSFTCTLRFDTEVELAYFDHGGILQYVIRNLI 896          

BLAST of aconitase vs. SwissProt
Match: gi|332278214|sp|Q94A28.3|ACO2M_ARATH (RecName: Full=Aconitate hydratase 2, mitochondrial; Short=Aconitase 2; Short=mACO2; AltName: Full=Citrate hydro-lyase 2; Flags: Precursor)

HSP 1 Score: 1115.52 bits (2884), Expect = 0.000e+0
Identity = 540/893 (60.47%), Postives = 668/893 (74.80%), Query Frame = 0

Query:   26 FGHLLRDLKVEGHGNL-KFFDLASLG-PEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIP---ADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            +  +L  L   G G   K++ L +L  P   +LPFS+R+LLESA+RNCDN+QV + DV KIL W +N S    VE+ F+PARVILQDFTGVP +VD A+MRDAV+ LG DP  INP  P DLV+DHS+QVDF R+ DA  KN ELEF+RNKERFTFLKWG+ AF+NML+VPPGSGIVHQVNLEYL RV F     +YPDSVVGTDSHTTMI+GLGV GWGVGGIEAEA MLGQP+SMVLP V+G+K+ GK+   VT+TD+VLT+T+ LR+ GVVGKFVEFYG G++ LS+ADRATIANM PEYGAT+G+FPVD  +++YLK T R    +  IE+YL A  M  +Y++  Q+  ++  ++LDL  V P +SGPKRPHDRV + DMK D+  CL   +GFKG+ +PK+K    V F  NG+   I HG+V+IAAITSCTNTSNPSVM+GA L+AKKA + GL V P++KTSL+PGS VV  YL  SG+   L   GF IVGYGC TCIGNSG L+  V+ AI+  D++   VLSGNRNFEGR+H  TRANYLASPPLV+AYA+AG VDIDFE E +G  +DGK V+LRD+WPS EE+  V +  V+P+MF+  Y  IT GN  WN+L AP   LY WD NSTYI  PP+F  MT   P PR+V++A  LLN  DSVTTDHISPAG+I + SPAA+FL  +G+   +FNSYGSRRGND VMARGTFANIR+VNK + G+ GP T+HIP+ E + VFDAA +YK   +  +ILAG +YG GSSRDWAAKGP L GV+AV+A+S+ERIHRSNL GMGI+PL + +GE+A++LGLT  E +TVH+P   +D++P Q + V     KSF    RFDTE+EL YY HGGIL Y+IR + A
Sbjct:  104 YKDILTSLPKPGGGEYGKYYSLPALNDPRIDKLPFSVRILLESAIRNCDNYQVTKDDVEKILDW-ENTSTKQ-VEIAFKPARVILQDFTGVPVLVDLASMRDAVKNLGSDPSKINPLVPVDLVVDHSIQVDFARSEDAAQKNLELEFKRNKERFTFLKWGSTAFQNMLVVPPGSGIVHQVNLEYLGRVVFNSKGFLYPDSVVGTDSHTTMIDGLGVAGWGVGGIEAEAAMLGQPMSMVLPGVVGFKLDGKLKEGVTATDLVLTVTQILRKHGVVGKFVEFYGEGMSELSLADRATIANMSPEYGATMGFFPVDHVTLEYLKLTGRSDETVSMIESYLRANNMFVDYNEPQQERAYTSYLQLDLGHVEPCISGPKRPHDRVPLKDMKADWHACLDNPVGFKGFAVPKEKQEEVVKFSYNGQPAEIKHGSVVIAAITSCTNTSNPSVMIGAALVAKKASDLGLKVKPWVKTSLAPGSRVVEKYLDRSGLRESLTKQGFEIVGYGCTTCIGNSGNLDPEVASAIEGTDIIPAAVLSGNRNFEGRVHPQTRANYLASPPLVVAYALAGTVDIDFEKEPIGTRSDGKSVYLRDVWPSNEEVAQVVQYSVLPSMFKSSYETITEGNPLWNELSAPSSTLYSWDPNSTYIHEPPYFKNMTANPPGPREVKDAYCLLNFGDSVTTDHISPAGNIQKTSPAAKFLMDRGVISEDFNSYGSRRGNDEVMARGTFANIRIVNKLLKGEVGPNTVHIPTGEKLSVFDAASKYKTAEQDTIILAGAEYGSGSSRDWAAKGPLLLGVKAVIAKSFERIHRSNLAGMGIIPLCFKAGEDAETLGLTGHERYTVHLPTKVSDIRPGQDVTVTTDSGKSFVCTLRFDTEVELAYYDHGGILPYVIRSLSA 994          

BLAST of aconitase vs. SwissProt
Match: gi|60392492|sp|Q62751.2|IREB2_RAT (RecName: Full=Iron-responsive element-binding protein 2; Short=IRE-BP 2; AltName: Full=Iron regulatory protein 2; Short=IRP2)

HSP 1 Score: 1022.31 bits (2642), Expect = 0.000e+0
Identity = 505/964 (52.39%), Postives = 681/964 (70.64%), Query Frame = 0

Query:   19 APSAPSPFGHLLRDLKVEGHGNLKFFDLASLG-PEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWA---QNQSVP-----------SGVE--VPFRPARVILQDFTGVPAVV----------------DFA--AMRDAVQRLGGD--------PEMINPQ-------------CPSDLVIDHS---------------VQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            +PSA   F +L+  L   G    KFF++  LG  +Y  LP+SIRVLLE+AVRNCD F + + DVI IL W     N  VP           +G+   V F   R  ++   G P  V                DF+  A+++A    GGD        P  + P+             C S  +  +S                 +  V  P+ + KN+E+EF RN+ER  F KW + AFKN+ ++PPG+G+ HQVNLE+L+RV F ++D+++PDSV+GTDSH TM+NGLG++GWGVGGIE EAVMLG P+++ LP+V+G +++G  ++ VTS D+VL ITKHLRQ+GV GKFVEF+G GV+ LSI DR TIANMCPEYGA + +FPVD+ ++ +L+ T  D TK++++E YL AV + RN  ++S+ P +S+V++++L S+V S+SGPKRP DRV+V+DMK+DF  CL+  +GFKG+ +  +K + TV    +G E+ +SHG+V+IAA+ SCTN  NPSVML AGL+AKKAVE GL V PYI+TSLSPGSG+VT+YL  SGV+PYL  LGF IVGYGC TC+GN+ PL E +  A+K+GDLV CGVLSGN+NFEGR+    RANYLASPPLV+AYAIAG V+IDF+TE LG D+ GK ++L DIWPSREE+  +E+++VI +MF+ +  K+  GN RWN L+AP+  L+PWD  STYI+ P FFD +TKE  A + +ENA  LL L DSVTTDHISPAGSIAR+S AA++L ++G+TPREFNSYG+RRGNDAVM RGTFANI+L NKFIG+  PKT+H PS + +DVF+AAE Y+KE   L+ILAG+ YG G+SRDWAAKGP+L GV+AV+AESYE+IH+ +L+G+GI PL+++ GENADSLGL+ +E F++  P +L P  TL ++ S  K F V+A F+ ++E+T Y+HGG+LN++ RK +
Sbjct:    3 SPSAGYTFEYLIETLN--GSSQKKFFNVPKLGGTKYDILPYSIRVLLEAAVRNCDGFLMKKEDVINILDWKTKQSNVEVPFFPARVVLQDFTGIPAMVDFAAMREAVKTLGGDPKKVHPACPTDLTVDHSLQIDFSKCAIQNAPNPGGGDLQKAGKLSPLKVQPKKLPCRGQTTCRGSCDSGELSRNSGTFSSQIENTPVLCPFHLQPVPEPETVLKNQEVEFGRNRERLQFFKWSSGAFKNVAVIPPGTGMAHQVNLEHLSRVVFEEADLLFPDSVIGTDSHITMVNGLGILGWGVGGIETEAVMLGLPVTLTLPEVVGCELTGSSNAFVTSIDIVLGITKHLRQVGVAGKFVEFFGSGVSQLSIVDRTTIANMCPEYGAILSFFPVDNVTLRHLEHTGFDKTKLESMEEYLKAVKLFRNDENSSE-PEYSQVIQINLNSIVASVSGPKRPQDRVAVTDMKSDFQACLNEKVGFKGFQVAAEKQSDTVSVRYDGSEYKLSHGSVVIAAVISCTNNCNPSVMLAAGLLAKKAVETGLRVKPYIRTSLSPGSGMVTHYLSSSGVLPYLSKLGFEIVGYGCSTCVGNTAPLSEAILNAVKQGDLVTCGVLSGNKNFEGRLCDCVRANYLASPPLVVAYAIAGTVNIDFQTEPLGTDSTGKNIYLHDIWPSREEVHQIEEEHVILSMFKALKEKVEMGNKRWNSLDAPDSVLFPWDVKSTYIRCPSFFDKLTKEPAASQPIENAHVLLYLGDSVTTDHISPAGSIARSSAAAKYLTNRGLTPREFNSYGARRGNDAVMTRGTFANIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIPLIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLEFLPGENADSLGLSGREVFSLSFPEELFPGITLNIKTSTGKEFSVIAAFENDVEITLYKHGGLLNFVARKFL 963          

BLAST of aconitase vs. SwissProt
Match: gi|308153591|sp|P48200.3|IREB2_HUMAN (RecName: Full=Iron-responsive element-binding protein 2; Short=IRE-BP 2; AltName: Full=Iron regulatory protein 2; Short=IRP2)

HSP 1 Score: 1012.68 bits (2617), Expect = 0.000e+0
Identity = 509/962 (52.91%), Postives = 672/962 (69.85%), Query Frame = 0

Query:   19 APSAPSPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWA---QNQSVP-----------SGVE--VPFRPARVILQDFTGVPAVV----------------DFA--AMRDAVQRLGGD--------PEMINPQ-------------CPSDLVIDHS---------------VQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKM 910
            AP A   F +L+  L    H   KFFD++ LG +Y  LP+SIRVLLE+AVRNCD F + + DV+ IL W     N  VP           +G+   V F   R  ++   G P  V                DF+  A+++A    GGD        P  + P+             C S  +  +S                 +  V  P+ + KN+E+EF RN+ER  F KW ++ FKN+ ++PPG+G+ HQ+NLEYL+RV F + D+++PDSVVGTDSH TM+NGLG++GWGVGGIE EAVMLG P+S+ LP+V+G +++G  +  VTS DVVL ITKHLRQ+GV GKFVEF+G GV+ LSI DR TIANMCPEYGA + +FPVD+ ++ +L+ T     K++++E YL AV + RN  ++S +P +S+V++++L S+VPS+SGPKRP DRV+V+DMK+DF  CL+  +GFKG+ +  +K    V     G E+ +SHG+V+IAA+ SCTN  NPSVML AGL+AKKAVE GL V PYI+TSLSPGSG+VT+YL  SGV+PYL  LGF IVGYGC  C+GN+ PL + V  A+K+GDLV CG+LSGN+NFEGR+    RANYLASPPLV+AYAIAG V+IDF+TE LG D  GK ++L DIWPSREE+  VE+++VI +MF+ +  KI  GN RWN LEAP+  L+PWD  STYI+ P FFD +TKE  A + +ENA  LL L DSVTTDHISPAGSIARNS AA++L ++G+TPREFNSYG+RRGNDAVM RGTFANI+L NKFIG+  PKT+H PS + +DVF+AAE Y+KE   L+ILAG+ YG G+SRDWAAKGP+L GV+AV+AESYE+IH+ +L+G+GI PLQ++ GENADSLGL+ +E F++  P +L P  TL +Q S  K F V+A F+ ++E+T Y+HGG+LN++ RK 
Sbjct:    3 APKAGYAFEYLIETLNDSSHK--KFFDVSKLGTKYDVLPYSIRVLLEAAVRNCDGFLMKKEDVMNILDWKTKQSNVEVPFFPARVLLQDFTGIPAMVDFAAMREAVKTLGGDPEKVHPACPTDLTVDHSLQIDFSKCAIQNAPNPGGGDLQKAGKLSPVKVQPKKLPCRGQTTCRGSCDSGELGRNSGTFSSQIENTPILCPFHLQPVPEPETVLKNQEVEFGRNRERLQFFKWSSRVFKNVAVIPPGTGMAHQINLEYLSRVVFEEKDLLFPDSVVGTDSHITMVNGLGILGWGVGGIETEAVMLGLPVSLTLPEVVGCELTGSSNPFVTSIDVVLGITKHLRQVGVAGKFVEFFGSGVSQLSIVDRTTIANMCPEYGAILSFFPVDNVTLKHLEHTGFSKAKLESMETYLKAVKLFRNDQNSSGEPEYSQVIQINLNSIVPSVSGPKRPQDRVAVTDMKSDFQACLNEKVGFKGFQIAAEKQKDIVSIHYEGSEYKLSHGSVVIAAVISCTNNCNPSVMLAAGLLAKKAVEAGLRVKPYIRTSLSPGSGMVTHYLSSSGVLPYLSKLGFEIVGYGCSICVGNTAPLSDAVLNAVKQGDLVTCGILSGNKNFEGRLCDCVRANYLASPPLVVAYAIAGTVNIDFQTEPLGTDPTGKNIYLHDIWPSREEVHRVEEEHVILSMFKALKDKIEMGNKRWNSLEAPDSVLFPWDLKSTYIRCPSFFDKLTKEPIALQAIENAHVLLYLGDSVTTDHISPAGSIARNSAAAKYLTNRGLTPREFNSYGARRGNDAVMTRGTFANIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIPLIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLQFLPGENADSLGLSGRETFSLTFPEELSPGITLNIQTSTGKVFSVIASFEDDVEITLYKHGGLLNFVARKF 962          

BLAST of aconitase vs. SwissProt
Match: gi|254806047|sp|B3VKQ2.1|IREB2_PIG (RecName: Full=Iron-responsive element-binding protein 2; Short=IRE-BP 2)

HSP 1 Score: 991.104 bits (2561), Expect = 0.000e+0
Identity = 455/755 (60.26%), Postives = 592/755 (78.41%), Query Frame = 0

Query:  156 VRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKM 910
            V  P+ + KN+E+EF RN+ER  F KW ++ FKN+ ++PPG+G+ HQVNLEYL+RV F + D+++PDSVVGTDSH TM+NGLG++GWGVGGIE EAVMLG P+S+ LP+V+G +++G  +  VTS DVVL ITKHLRQIGV GKFVEF+G GV+ LSI DR TIANMCPEYGA + +FPVD+ ++ +L+ T  +  K+K++E YL AV + RN  D S +P +S+V++++L S+VPS+SGPKRP DRV+V+DMK+DF  CL+  +GFKG+ +  +K   TV     G E+ +SHG+V+IAA+ SCTN  NPSVML AGL+AKKAVE GL V PYI+TSLSPGSG+VT+YL  SGV+PYL  LGF IVGYGC TC+GN+ PL E V  A+K+GDLV CGVLSGN+NFEGR+    RANYLASPPLV+AYAIAG V+IDF TE LG D  GK ++L DIWPSREE+  +E+++V+ +MF+ +  KI  GN RWN LEAP+  L+PWD  STYI+ P FFD +TKE  A + +ENA  LL L DSVTTDHISPAGSIAR+S AA++L ++G+TPREFNSYG+RRGNDAVM RGTFANI+L NKFIG+  PKT+H PS + +DVF+AAE Y+KE   L+ILAG+ YG G+SRDWAAKGP+L GV+AV+AESYE+IH+ +L+G+GI PLQ++ GENADSLGL+ +E F++  P +L P  TL ++ S  K F V+A F+ ++E+  Y+HGG+LN++ RK 
Sbjct:  209 VPEPETVLKNQEVEFGRNRERLQFFKWSSRVFKNVAVIPPGTGMAHQVNLEYLSRVVFEEKDLLFPDSVVGTDSHITMVNGLGILGWGVGGIETEAVMLGLPVSLTLPEVVGCELTGSSNPFVTSIDVVLGITKHLRQIGVAGKFVEFFGSGVSQLSIVDRTTIANMCPEYGAILSFFPVDNVTLKHLEYTGFNKAKLKSMETYLKAVKLFRNDQDNSGEPEYSQVIQINLNSIVPSVSGPKRPQDRVAVTDMKSDFQACLNEKVGFKGFQIAAEKQNDTVSIHYEGSEYKLSHGSVVIAAVISCTNNCNPSVMLAAGLLAKKAVEAGLRVKPYIRTSLSPGSGMVTHYLSSSGVLPYLSKLGFEIVGYGCSTCVGNTAPLSEAVLNAVKQGDLVTCGVLSGNKNFEGRLCDCVRANYLASPPLVVAYAIAGTVNIDFRTEPLGTDPTGKNIYLHDIWPSREEVHQIEEEHVVLSMFKALKEKIEMGNKRWNSLEAPDSVLFPWDLKSTYIRCPSFFDKLTKEPVALQPIENAHVLLYLGDSVTTDHISPAGSIARSSAAAKYLTNRGLTPREFNSYGARRGNDAVMTRGTFANIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIPLIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLQFLPGENADSLGLSGRETFSLTFPEELSPGVTLNIKTSTGKIFSVIASFENDVEIILYKHGGLLNFVARKF 963          

HSP 2 Score: 157.918 bits (398), Expect = 3.097e-38
Identity = 78/138 (56.52%), Postives = 100/138 (72.46%), Query Frame = 0

Query:   19 APSAPSPFGHLLRDLKVEGHGNLKFFDLASLG-PEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDF 155
            APSA   F +L+  L    H   KFF++  LG  +Y  LP+SIRVLLE+AVRNCD F + + DV+ IL W   QS    VEVPF P RV+LQDFTG+PA+VDFAAMR+AV+ LGGDP+ ++P CP+DL +DHS+Q+DF
Sbjct:    3 APSAGYAFEYLIETLNDSSHK--KFFNVPRLGGTKYDVLPYSIRVLLEAAVRNCDGFLMKKEDVMNILDWKTKQS---NVEVPFFPGRVLLQDFTGIPAMVDFAAMREAVKTLGGDPKKVHPACPTDLTVDHSLQIDF 135          

BLAST of aconitase vs. SwissProt
Match: gi|341940838|sp|Q811J3.2|IREB2_MOUSE (RecName: Full=Iron-responsive element-binding protein 2; Short=IRE-BP 2; AltName: Full=Iron regulatory protein 2; Short=IRP2)

HSP 1 Score: 987.252 bits (2551), Expect = 0.000e+0
Identity = 451/756 (59.66%), Postives = 599/756 (79.23%), Query Frame = 0

Query:  156 VRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            V  P+ + KN+E+EF RN+ER  F KW + AFKN+ ++PPG+G+ HQVNLEYL+RV F ++D+++PDSVVGTDSH TM+NGLG++GWGVGGIE EAVMLG P+++ LP+V+G +++G  ++ VTS D+VL ITKHLRQ+GV GKFVEF+G GV+ LSI DR TIANMCPEYGA + +FPVD+ ++ +L+ T  D TK++++E YL AV + RN  + S +P +S+V++++L S+V S+SGPKRP DRV+V+DMK+DF  CL+  +GFKG+ +  +K + TV    +G E+ +SHG+V+IAA+ SCTN  NPSVML AGL+AKKAVE GL V PYI+TSLSPGSG+VT+YL  SGV+PYL  LGF IVGYGC TC+GN+ PL E V  A+K+GDLV CGVLSGN++FEGR+    RANYLASPPLV+AYAIAG V+IDF+TE LG D+ GK ++L DIWPSREE+  +E+++VI +MF+ +  K+  GN RWN LEAP+  L+PWD  STYI+ P FFD +TKE  A + +ENA  LL L DSVTTDHISPAGSIAR+S AA++L ++G+TPREFNSYG+RRGNDAVM RGTFANI+L NKFIG+  PKT+H PS + +DVF+AAE Y+KE   L+ILAG+ YG G+SRDWAAKGP+L GV+AV+AESYE+IH+ +L+G+GI PL+++ GENADSLGL+ +E F++  P +L P  TL ++ S  K F V+A F  ++E+T Y+HGG+LN++ RK +
Sbjct:  209 VPEPETVLKNQEVEFGRNRERLQFFKWSSGAFKNVAVIPPGTGMAHQVNLEYLSRVVFEETDLLFPDSVVGTDSHITMVNGLGILGWGVGGIETEAVMLGLPVTLTLPEVVGCELTGSSNAFVTSIDIVLGITKHLRQVGVAGKFVEFFGSGVSQLSIVDRTTIANMCPEYGAILSFFPVDNVTLRHLEHTGFDKTKLESMEKYLKAVKLFRN-DENSSEPEYSQVIQINLNSIVASVSGPKRPQDRVAVTDMKSDFQACLNEKVGFKGFQVAAEKQSDTVSVRYDGSEYKLSHGSVVIAAVISCTNNCNPSVMLAAGLLAKKAVEIGLRVKPYIRTSLSPGSGMVTHYLSSSGVLPYLSKLGFDIVGYGCSTCVGNTAPLSEAVLNAVKQGDLVTCGVLSGNKHFEGRLCDCVRANYLASPPLVVAYAIAGTVNIDFQTEPLGTDSTGKEIYLHDIWPSREEVHQMEEEHVILSMFKTLKEKVEMGNKRWNSLEAPDSVLFPWDVKSTYIRCPSFFDKLTKEPAASQPIENAHVLLYLGDSVTTDHISPAGSIARSSAAAKYLTNRGLTPREFNSYGARRGNDAVMTRGTFANIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIPLIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLEFLPGENADSLGLSGREVFSLSFPEELFPGITLNIKTSTGKEFSVIASFANDVEITLYKHGGLLNFVARKFL 963          

HSP 2 Score: 159.844 bits (403), Expect = 7.952e-39
Identity = 78/140 (55.71%), Postives = 103/140 (73.57%), Query Frame = 0

Query:   19 APSAPSPFGHLLRDLKVEGHGNLKFFDLASLG-PEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVR 157
            +PSA   F +L+  L   G+   KFF++  LG  +Y  LP+SIRVLLE+AVRNCD F + + DV+ IL W   QS    VEVPF PARV+LQDFTG+PA+VDFAAMR+AV+ LGGDP+ ++P CP+DL +DHS+Q+DF +
Sbjct:    3 SPSAGYTFEYLIETLN--GNSQKKFFNVPKLGGTKYDILPYSIRVLLEAAVRNCDGFLMKKEDVMNILDWKTKQS---NVEVPFFPARVVLQDFTGIPAMVDFAAMREAVKTLGGDPKKVHPACPTDLTVDHSLQIDFSK 137          

BLAST of aconitase vs. SwissProt
Match: gi|81624827|sp|Q9RTN7.1|ACNA_DEIRA (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein; Flags: Precursor)

HSP 1 Score: 978.393 bits (2528), Expect = 0.000e+0
Identity = 497/882 (56.35%), Postives = 647/882 (73.36%), Query Frame = 0

Query:   41 LKFFDLASL-GPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFT----DSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGK----KSFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            L F++L  L G +  +LP SI+VLLES +R  +++ V   DV  +  W+     P  VE+PF+PARVILQDFTGVPAVVD AAMR A+ +LGGDP  INP  P DLVIDHSVQVD   T  AL+ N  LEFERN+ER+ FL+WG +AF N  +VPP SGIVHQVNLEYLA+   +    D ++VYPDS+VGTDSHTTMINGLG+VGWGVGGIEAEAVMLGQPI M++P+V+G+KI+G M    T+TD+ L +T+ LR+ GVVGKFVEFYG G++++++ DRATIANM PEYGAT+G+FPVD +++ YL++T R   +I  +EAY  A GM R  +D + DP F++ +ELDLA++VPSL+GPKRP DRV++SDM + F   L+A +  +G+ L  DKL       + G +  I HGAV +A+ITSCTNTSNPSV++ AGL+AKKAVEKGL   P++KTSL+PGS VVT YL+ +G+  YLD +GF+ VGYGCMTCIGNSGPL EPV EAI++GDLV   VLSGNRNFEGR++   +ANYLASPPLV+AYA+AG V  D   +A+GQD++G+ VFL+DIWP+  EIQ    + +   MF++VY  I   N  WN +   EG L+ W ++STYI++PPFFD +         ++ A  L+ + DSVTTDHISPAGS   ++PA R+L  +GI P++FNSYGSRRGND +M RGTFANIRL N+   G  G  T +  + EV  +FDA+  YK+    LV+LAG+DYG GSSRDWAAKG FL GV+AV+AES+ERIHRSNLVGMG++PLQY +GE ADSLG+   E F   +P DLKP+Q + V+V+GK    +   V+ R DT +E+ YY++GGIL  ++R +++
Sbjct:   24 LYFYNLNKLQGHDVSRLPVSIKVLLESVLREANDYDVRREDVETVAGWSATN--PE-VEIPFKPARVILQDFTGVPAVVDLAAMRSAMVKLGGDPSKINPLIPVDLVIDHSVQVDEFGTEFALANNMALEFERNRERYEFLRWGQQAFDNFGVVPPASGIVHQVNLEYLAKGVQSRAEDDGEVVYPDSLVGTDSHTTMINGLGIVGWGVGGIEAEAVMLGQPIYMLMPEVIGFKITGAMPEGATATDLALRVTQMLREKGVVGKFVEFYGAGLSNMTLPDRATIANMAPEYGATMGFFPVDDEALRYLRRTGRLEDEIGLVEAYYKAQGMFR--TDETPDPVFTDTIELDLATIVPSLAGPKRPQDRVNLSDMHSVFNEALTAPVKNRGFELGSDKLDAQ--GTIGGTDIKIGHGAVTLASITSCTNTSNPSVLIAAGLVAKKAVEKGLKTKPWVKTSLAPGSRVVTEYLETAGLQQYLDQIGFNTVGYGCMTCIGNSGPLPEPVVEAIQEGDLVVASVLSGNRNFEGRVNPHIKANYLASPPLVVAYALAGTVVNDIVNDAIGQDSNGQDVFLKDIWPTNAEIQEAMDRSINAEMFKKVYDGIEKSNADWNAIPVAEGALFDWKEDSTYIQNPPFFDTLAGGAHEIESIKGARALVKVGDSVTTDHISPAGSFKADTPAGRYLTERGIAPKDFNSYGSRRGNDRIMTRGTFANIRLKNQLAPGTEGGFTTNFLNGEVTSIFDASTAYKEAGVPLVVLAGKDYGMGSSRDWAAKGTFLLGVKAVIAESFERIHRSNLVGMGVLPLQYKNGETADSLGINGDETFEFVLPGDLKPRQDVTVKVTGKDGNTRDITVMCRIDTPVEIDYYKNGGILQTVLRGILS 898          

BLAST of aconitase vs. SwissProt
Match: gi|82082649|sp|Q5ZLQ4.1|IREB2_CHICK (RecName: Full=Iron-responsive element-binding protein 2; Short=IRE-BP 2)

HSP 1 Score: 972.23 bits (2512), Expect = 0.000e+0
Identity = 443/756 (58.60%), Postives = 585/756 (77.38%), Query Frame = 0

Query:  156 VRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            V  P+ + KN+E+EF RN+ER  F KW +K FKN  I+PP +G+ HQVNLEYL+RV F   D +YPDSVVGTDSHTTM+NGLG++GWGVGGIE EAVMLG P+++ LP+V+G +++G    L TS D+VL ITKHLRQ  V GKFVEF+G GV+ LS+ADR TIANMCPEYGA + +FPVD+ ++ +L+ T  D  K++ +EAYL AV + RN   +S++P +S+VV++ L+S++P +SGPKR  DRV+V++MK+DF  CL+   G KG+ +  +K    VP    G ++ +SHG V+IAA+ SCTN  NPSVML AGL+AKKAVE GL V PYI+TSLSPGSG+VT+YL  SGV+PYL  LGF +VGYGC TC+GN+ PL E +  AIK+GD++ CGVLSG +NFEGR+    RANYLASPPLV+AYAIAG V IDFETE LG   +G+ ++LRDIWP+R+E+  VE++ VI +MF+E+  K+  GN RWN LEAPE  L+PWD  STYI+ P FFD + KE  + + +ENA  LL L DSVTTDHISPAGSIAR+S AA++L +KG+TPREFNSYG+RRGNDAVM RGTFANI+L+NKFIG+  PKT+H PS + +DVF+AAE Y+KE   ++ILAG+ YG GSSRDWAAKGPFL GV+AV+AESYE++H+S L+G+GI PLQ++ GEN ++LGLT +E+F++  P +L P+ TL+++ S  K F V A F+ ++E+T Y++GG LN++ R+ +
Sbjct:  210 VPEPETVLKNQEMEFGRNRERLQFFKWSSKVFKNTSIIPPETGMAHQVNLEYLSRVVFDVEDFLYPDSVVGTDSHTTMVNGLGILGWGVGGIETEAVMLGMPVTLTLPEVVGCELTGTASPLATSIDIVLGITKHLRQAEVAGKFVEFFGSGVSQLSVADRTTIANMCPEYGAILSFFPVDNVTLKHLRHTGFDEAKLEVMEAYLKAVKLFRNGESSSREPEYSQVVQISLSSIIPHVSGPKRSQDRVAVNNMKSDFQTCLNEKAGVKGFQIAAEKQNDVVPVQYEGNQYELSHGCVVIAAVISCTNNCNPSVMLAAGLLAKKAVEAGLEVKPYIRTSLSPGSGMVTHYLSSSGVLPYLSKLGFEVVGYGCSTCVGNTAPLPEAIRNAIKQGDIIACGVLSGTKNFEGRLCDCVRANYLASPPLVVAYAIAGTVRIDFETEPLGTGFNGRSIYLRDIWPTRKELHTVEEECVISSMFKELKEKMEKGNKRWNSLEAPESPLFPWDLKSTYIRCPSFFDKLAKEPVSLQPIENAHVLLYLGDSVTTDHISPAGSIARSSAAAKYLTNKGLTPREFNSYGARRGNDAVMTRGTFANIKLLNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIPVIILAGKKYGLGSSRDWAAKGPFLLGVKAVLAESYEKVHKSQLIGIGIAPLQFLPGENPNTLGLTGREQFSILFPPELSPKMTLDIKTSTGKVFSVFALFENDVEITLYKNGGSLNFVARRFL 965          

HSP 2 Score: 152.525 bits (384), Expect = 1.414e-36
Identity = 71/135 (52.59%), Postives = 99/135 (73.33%), Query Frame = 0

Query:   24 SPFGHLLRDLKVEGHGNLKFFDLASLG-PEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVR 157
            SP+  ++ +L+   +   +F++++ LG  +Y  LP+SIRVL ES++RNCD F V ETD + IL W   Q+    VEVPF PARV+LQDFTG+PA+VDFAAMR+AV+  GGDP  +NP CP+DL +DHS+Q+DF +
Sbjct:    8 SPYQPIIEELR--NYPQKRFYNVSKLGGTKYDVLPYSIRVLFESSIRNCDGFLVKETDAMNILDWKTKQN---DVEVPFCPARVVLQDFTGIPAMVDFAAMREAVRNAGGDPVKVNPACPTDLTVDHSLQIDFSK 137          

BLAST of aconitase vs. SwissProt
Match: gi|81600603|sp|Q5SMF6.1|ACNA_THET8 (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 959.518 bits (2479), Expect = 0.000e+0
Identity = 490/889 (55.12%), Postives = 650/889 (73.12%), Query Frame = 0

Query:   39 GNLKFFDLASLG----PEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDS----DIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVEN---ALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEV----QVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            G   ++DL  L      E  +LPFSIRV+LES +RN D +QV   D+  + +W  +   P  + VP + ARVILQDFTGVPAVVD AAMRDA++  GGDP+ INP  P+DLVIDHSVQVD   T  A   N E E+ERN+ER+  LKW   A +N  +VPPG+GIVHQVN+EYL +V  T       + +PDS+VGTDSHTTM+NGLGV+GWGVGGIEAEAVMLGQP  M+ P+V+G+K+ G++    T+TD+VLT+T+ LR+ GVVGKFVEFYGPGVA LS  DRATIANM PEYGAT+G+FPVD ++++YL+QT R    ++ +EAY  AVG+ R   +A +   +SE +ELDL++V PSL+GPKRP DRV + ++K  FL  L+  +  +G+GL +D+L   V      +E  ++HG+V+IAAITSCTNTSNPSVMLGAGL+AKKAVE GL   P++KTSL+PGS VVT YL+ SG++P+L+ALGF +VGYGC TCIGNSGPL E +++A+++G+LV   VLSGNRNFEGRI+   +ANYLASP LV+AYA+AGR+DIDF TE LG D +GKP++L+DIWPS EEI+   +K + P +F++ YSK+  G++RW  L AP G+LY WD  STYI++PPFF+ + +     RKVE+   A  LL L DSVTTDHISPAG+I   SPA ++L SKG+ P +FNSYGSRRGN  VM RGTFANIR+ N  + G  G     +P  +V  V++ A  YK E   L+++AG++YG GSSRDWAAKG +L G+RAV+AES+ERIHRSNLVGMG++PL+++ GEN ++LGLT  E + +    DLKP++ +++    +   +  F+ +AR DT +E+ YY++GGIL  ++  M+
Sbjct:   16 GTYGYYDLQELERKGVAEVSRLPFSIRVMLESLLRNEDGYQVTREDIEALARWRPD---PGEINVPLKLARVILQDFTGVPAVVDLAAMRDAIKAKGGDPKRINPVVPADLVIDHSVQVDAFGTAYAFFYNVEKEYERNRERYLLLKWAQNALENFRVVPPGTGIVHQVNIEYLTKVVMTGKRDGLTLAFPDSLVGTDSHTTMVNGLGVLGWGVGGIEAEAVMLGQPYYMLAPRVVGFKLYGELPEGATATDLVLTVTEMLRKHGVVGKFVEFYGPGVAKLSTPDRATIANMAPEYGATMGFFPVDEETLNYLRQTGRPEELVELVEAYTKAVGLFRT-PEAEEKVQYSEYLELDLSAVEPSLAGPKRPQDRVPLKEVKKSFLAHLTKPVKERGFGLSEDQLQRKVLVKRRDEEFELTHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLDRKPWVKTSLAPGSKVVTDYLEMSGLMPFLEALGFHLVGYGCTTCIGNSGPLPEDIAKAVEEGNLVVAAVLSGNRNFEGRINPHVKANYLASPMLVVAYALAGRMDIDFTTEPLGFDPNGKPIYLKDIWPSMEEIREAIRKTLDPELFKKEYSKVFEGDERWQALPAPTGELYQWDPESTYIQNPPFFEDLGE-----RKVEDIRGARVLLVLGDSVTTDHISPAGAIPVKSPAGQYLISKGVKPEDFNSYGSRRGNHEVMMRGTFANIRIKNLMLDGIEGGYAKKLPEGDVDFVYNVAMRYKAEGTPLLVIAGKEYGTGSSRDWAAKGTYLLGIRAVLAESFERIHRSNLVGMGVLPLEFLPGENRETLGLTGYEVYDILGLEDLKPRKLVDIVARREDGSEVRFQAIARLDTPVEVDYYKNGGILQTVLLNML 895          

BLAST of aconitase vs. nr
Match: gi|1227979290|ref|XP_021921229.1| (cytoplasmic aconitate hydratase-like isoform X2 [Zootermopsis nevadensis] >gi|1227979292|ref|XP_021921230.1| cytoplasmic aconitate hydratase-like isoform X3 [Zootermopsis nevadensis])

HSP 1 Score: 1384.39 bits (3582), Expect = 0.000e+0
Identity = 669/882 (75.85%), Postives = 763/882 (86.51%), Query Frame = 0

Query:   30 LRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            L+ L V+G    KFFDL SLG  Y +LPFSIRVLLESAVRNCDNFQV E DV  IL W ++Q+VP GVEV F+PARVILQDFTGVPAVVDFAAMRDAV++LGGDP+ INP CPSDLVIDHSVQVDFVRTPDAL KN++LEFERNKERF FLKWGAKAF+NMLIVPPGSGIVHQVNLEYLARV F    ++YPDSVVGTDSHTTMINGLGV+GWGVGGIEAEAVMLGQ ISM+LPKV+GYKI+G+++   TSTD+VLTITKHLRQ+GVVGKFVEF+G GVA LSIADRATI+NMCPEYGATVG+FPVD  S+ YL+QTNR   K++ +E YL +V M RNY+D S+DP FSEVV LDLA+VV S+SGPKRPHDRV V DM TDF  CL+  IGFKG+GLP+DKL  +  F+  G+E+V+ HG+V+IAAITSCTNTSNPSVMLGAGL+AKKA++ GL+V PYIKTSLSPGSGVVTYYLKESGVIPYL ALGF IVGYGCMTCIGNSGPL E ++EAI+K DLVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAGRVDIDFE E LG   DGKPVFL +IWP R EI  VE+KYVIP MF EVY+KI TG+  W  L+APEGKLYPWD +STYIK+PPFF+ MTKELP    +E A  LLNL DSVTTDHISPAGSIARNSPAAR+L ++G+TPREFNSYGSRRGNDAVMARGTFANIRLVNKF+GQAGPKT+HIPS E+MDVFDAAE Y+KE   L+ L G+DYG GSSRDWAAKGPFL G+RAV+AESYERIHRSNLVGMGIVPLQY+ G+NA+SLGLT KE++T+ IP +++P Q L V++   KSF+V+ RFDTE++LTY+RHGGILNYMIRK++
Sbjct:   48 LKKLSVDGK-EYKFFDLPSLGSSYDKLPFSIRVLLESAVRNCDNFQVKEQDVEAILDWEKSQAVPEGVEVAFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPDRINPVCPSDLVIDHSVQVDFVRTPDALQKNQDLEFERNKERFMFLKWGAKAFQNMLIVPPGSGIVHQVNLEYLARVVFDSEGVLYPDSVVGTDSHTTMINGLGVLGWGVGGIEAEAVMLGQAISMLLPKVVGYKITGQLNQYATSTDLVLTITKHLRQLGVVGKFVEFFGSGVAELSIADRATISNMCPEYGATVGFFPVDKNSLAYLRQTNRSENKVRLMEEYLKSVRMFRNYTDESEDPVFSEVVTLDLATVVSSVSGPKRPHDRVPVVDMPTDFRQCLTNKIGFKGFGLPEDKLNASGSFIHEGREYVLHHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAIDAGLTVLPYIKTSLSPGSGVVTYYLKESGVIPYLTALGFDIVGYGCMTCIGNSGPLPESIAEAIEKNDLVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGRVDIDFEKEPLGHSPDGKPVFLHEIWPLRNEIHDVEQKYVIPAMFREVYAKIETGSSNWANLKAPEGKLYPWDPSSTYIKNPPFFEAMTKELPNIEPIEEARVLLNLGDSVTTDHISPAGSIARNSPAARYLAARGLTPREFNSYGSRRGNDAVMARGTFANIRLVNKFVGQAGPKTIHIPSGEMMDVFDAAERYRKEKVPLIALVGKDYGSGSSRDWAAKGPFLLGLRAVIAESYERIHRSNLVGMGIVPLQYLEGQNAESLGLTGKEKYTIRIPENIRPLQELTVELVNGKSFQVVVRFDTEVDLTYFRHGGILNYMIRKIL 928          

BLAST of aconitase vs. nr
Match: gi|758375084|gb|AJO69941.1| (IRP1A [Lepeophtheirus salmonis])

HSP 1 Score: 1383.62 bits (3580), Expect = 0.000e+0
Identity = 663/895 (74.08%), Postives = 776/895 (86.70%), Query Frame = 0

Query:   24 SPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVL-NGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFI-GQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVS----GKKSFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            +PF  +L++ ++ G    K++D++SL P Y +LP+SIR+LLESAVRNCDNFQV E DV  IL W  N+      E+PFRP RVILQDFTGVPA+VDFAAMRDAV++LGGDP  INP CP+DLVIDHS+QVDF R   AL KNE+LEF+RNKERFTFLKWGA +F+NMLI+PPGSGIVHQVNLEYLARVCFTD+D+V+PDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLP+V+GY+ISG    LV STD+VLTITKHLRQ+GVVG+FVEFYGPG+  LSIADRATI+NMCPEYGAT+GYFPVD  SI+YL+ TNR+++K+K I+ YL+   ++R+Y D S DPTFS++V LDL +VV S SGPKRP DRV+V+DMK DFL CLS  IGFKG+ +P +K   TVP +  + KE+++ HG+VLIAAITSCTNTSNPSVMLGAGL+AKKAV+KGL+V PYIKTSLSPGSGVVTYYLK SGVIPYLDALGFSIVGYGCMTCIGNSGPLE+ V E + KGDLVC GVLSGNRNFEGRI+  TRANYLASPPLVIAYAIAGRVDIDFE EALG DA+ +P++LRDIWP+R+EIQ VEKKY++P+MFE+VYSK+TTGN+RWN LEAP+G LYPWD  STYIKSPPFFD M++ELP P+K++NAL LLNL DS TTDHISPAGSIARNSPAAR+L SK I+PREFNSYGSRRGNDA+MARGTFANIRLVNKF+ G+ GPKT+H+ S ++MDVFDAA EYKKEN+HL++LAG+DYGCGSSRDWAAKGPFL GVRAV+AESYERIHRSNLVGMGIVPLQY  GE+++SLG + KE+F+V +P +L PQQ L+++V       KSFK   RFDTELELTYYRHGGILNYMIRK+I+
Sbjct:    3 NPFDKILKNFEIGGK-TYKYYDVSSLDPSYHKLPYSIRILLESAVRNCDNFQVYEKDVETILNWEGNRG--KNAEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTDLVFPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGYFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAVTDMKNDFLQCLSNKIGFKGFAIPDNKKNITVPIIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGCMTCIGNSGPLEDYVVEGVNKGDLVCAGVLSGNRNFEGRINPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDANNEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTTGNERWNSLEAPKGILYPWDIESTYIKSPPFFDNMSEELPEPKKIKNALALLNLGDSFTTDHISPAGSIARNSPAARYLASKNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDGSIKSFKATVRFDTELELTYYRHGGILNYMIRKVIS 894          

BLAST of aconitase vs. nr
Match: gi|1227979288|ref|XP_021921228.1| (cytoplasmic aconitate hydratase-like isoform X1 [Zootermopsis nevadensis])

HSP 1 Score: 1373.61 bits (3554), Expect = 0.000e+0
Identity = 669/900 (74.33%), Postives = 763/900 (84.78%), Query Frame = 0

Query:   30 LRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRT------------------PDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            L+ L V+G    KFFDL SLG  Y +LPFSIRVLLESAVRNCDNFQV E DV  IL W ++Q+VP GVEV F+PARVILQDFTGVPAVVDFAAMRDAV++LGGDP+ INP CPSDLVIDHSVQVDFVRT                  PDAL KN++LEFERNKERF FLKWGAKAF+NMLIVPPGSGIVHQVNLEYLARV F    ++YPDSVVGTDSHTTMINGLGV+GWGVGGIEAEAVMLGQ ISM+LPKV+GYKI+G+++   TSTD+VLTITKHLRQ+GVVGKFVEF+G GVA LSIADRATI+NMCPEYGATVG+FPVD  S+ YL+QTNR   K++ +E YL +V M RNY+D S+DP FSEVV LDLA+VV S+SGPKRPHDRV V DM TDF  CL+  IGFKG+GLP+DKL  +  F+  G+E+V+ HG+V+IAAITSCTNTSNPSVMLGAGL+AKKA++ GL+V PYIKTSLSPGSGVVTYYLKESGVIPYL ALGF IVGYGCMTCIGNSGPL E ++EAI+K DLVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAGRVDIDFE E LG   DGKPVFL +IWP R EI  VE+KYVIP MF EVY+KI TG+  W  L+APEGKLYPWD +STYIK+PPFF+ MTKELP    +E A  LLNL DSVTTDHISPAGSIARNSPAAR+L ++G+TPREFNSYGSRRGNDAVMARGTFANIRLVNKF+GQAGPKT+HIPS E+MDVFDAAE Y+KE   L+ L G+DYG GSSRDWAAKGPFL G+RAV+AESYERIHRSNLVGMGIVPLQY+ G+NA+SLGLT KE++T+ IP +++P Q L V++   KSF+V+ RFDTE++LTY+RHGGILNYMIRK++
Sbjct:   48 LKKLSVDGK-EYKFFDLPSLGSSYDKLPFSIRVLLESAVRNCDNFQVKEQDVEAILDWEKSQAVPEGVEVAFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPDRINPVCPSDLVIDHSVQVDFVRTHRPDLDMNGQVAECQYSKPDALQKNQDLEFERNKERFMFLKWGAKAFQNMLIVPPGSGIVHQVNLEYLARVVFDSEGVLYPDSVVGTDSHTTMINGLGVLGWGVGGIEAEAVMLGQAISMLLPKVVGYKITGQLNQYATSTDLVLTITKHLRQLGVVGKFVEFFGSGVAELSIADRATISNMCPEYGATVGFFPVDKNSLAYLRQTNRSENKVRLMEEYLKSVRMFRNYTDESEDPVFSEVVTLDLATVVSSVSGPKRPHDRVPVVDMPTDFRQCLTNKIGFKGFGLPEDKLNASGSFIHEGREYVLHHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAIDAGLTVLPYIKTSLSPGSGVVTYYLKESGVIPYLTALGFDIVGYGCMTCIGNSGPLPESIAEAIEKNDLVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGRVDIDFEKEPLGHSPDGKPVFLHEIWPLRNEIHDVEQKYVIPAMFREVYAKIETGSSNWANLKAPEGKLYPWDPSSTYIKNPPFFEAMTKELPNIEPIEEARVLLNLGDSVTTDHISPAGSIARNSPAARYLAARGLTPREFNSYGSRRGNDAVMARGTFANIRLVNKFVGQAGPKTIHIPSGEMMDVFDAAERYRKEKVPLIALVGKDYGSGSSRDWAAKGPFLLGLRAVIAESYERIHRSNLVGMGIVPLQYLEGQNAESLGLTGKEKYTIRIPENIRPLQELTVELVNGKSFQVVVRFDTEVDLTYFRHGGILNYMIRKIL 946          

BLAST of aconitase vs. nr
Match: gi|1183238293|ref|XP_020710808.1| (cytoplasmic aconitate hydratase-like [Athalia rosae])

HSP 1 Score: 1359.36 bits (3517), Expect = 0.000e+0
Identity = 641/888 (72.18%), Postives = 756/888 (85.14%), Query Frame = 0

Query:   24 SPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            +P+ +LL+ +K+ G  + +++D+ + G +Y +LPFSIRVLLESAVRNCD FQV ++DV KIL+W  NQ +P GVEV F+PARVILQDFTGVPAVVDFAAMRDAV++LGGDP+ INP CPSDLVIDHSVQVDF R  +AL KNE+LEFERNKERF FLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARV F  + ++YPDSVVGTDSHTTMINGLGV+GWG GGIEAEAVMLGQ ISM+LP V+GYK+ G ++  VTSTD+VLTITK+LRQ+GVVGKFVEF+GPGVA LSIADRATI+NMCPEYGATVG+F VD QS+ YL+QT+R    +K IE YL  V M+RNY+D+SQDP FSEVV LDL++VV S+SGPKRPHDRVSVS+MK DF +CLSA IGFKG+GL  +KL     F   GK++ ++HGAV+IAAITSCTNTSNPSVMLGAGL+A+KAV+ GL+VAPYIKTSLSPGSGVVTYYLKESGVIP L  LGF +VGYGCMTCIGNSGPL + + +AI+K DLVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAG V IDFE E LG  ADGK ++LRD+WP+R EIQAVE+K+VIP MF+EVYSKI +G+  W+ L+AP G+LYPWD  STYIK PP+FDG+TK +P    +  A  LLNL DSVTTDHISPAGSIARNSPAAR+L  +G+ P+EFNSYGSRRGNDAVMARGTFANIRLVNKFIG+AGP+T++IP+NE MD+FDAAE+Y ++N  L+ L G++YG GSSRDWAAKGPFLQG++AV+AESYERIHRSNLVGMGI+PLQY+ GE+A+SLGLT  E + + IP D +P QT+EV     K F VL RFDTE++LTY+RHGGILNYMIR M+
Sbjct:    6 NPYKNLLKTIKI-GETDCQYYDVTNFGDKYDKLPFSIRVLLESAVRNCDEFQVKKSDVEKILEWETNQKIPEGVEVAFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPDKINPICPSDLVIDHSVQVDFTRGDNALKKNEDLEFERNKERFMFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVVFDANGLLYPDSVVGTDSHTTMINGLGVLGWGCGGIEAEAVMLGQAISMLLPNVVGYKLEGVLNQYVTSTDLVLTITKNLRQLGVVGKFVEFFGPGVAELSIADRATISNMCPEYGATVGFFAVDEQSLAYLRQTSRSDKHVKYIEEYLRTVKMLRNYNDSSQDPVFSEVVTLDLSTVVSSVSGPKRPHDRVSVSEMKKDFTSCLSAKIGFKGFGLKPEKLKVEATFQFEGKDYKLNHGAVVIAAITSCTNTSNPSVMLGAGLLAQKAVKAGLTVAPYIKTSLSPGSGVVTYYLKESGVIPSLTQLGFDVVGYGCMTCIGNSGPLPDSMIDAIEKNDLVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGTVLIDFEAEPLGYRADGKAIYLRDVWPTRAEIQAVEQKHVIPAMFKEVYSKIESGSGSWSSLQAPGGQLYPWDDESTYIKHPPYFDGLTKTIPPTNPIVKARVLLNLGDSVTTDHISPAGSIARNSPAARYLAKRGLKPKEFNSYGSRRGNDAVMARGTFANIRLVNKFIGKAGPRTIYIPTNEEMDIFDAAEKYAEDNVPLIALVGKEYGSGSSRDWAAKGPFLQGIKAVIAESYERIHRSNLVGMGIIPLQYLPGESAESLGLTGLELYNISIPDDYRPGQTIEVTTDDGKKFNVLVRFDTEVDLTYFRHGGILNYMIRTMV 892          

BLAST of aconitase vs. nr
Match: gi|939243160|ref|XP_014240878.1| (cytoplasmic aconitate hydratase-like isoform X2 [Cimex lectularius])

HSP 1 Score: 1350.5 bits (3494), Expect = 0.000e+0
Identity = 651/892 (72.98%), Postives = 757/892 (84.87%), Query Frame = 0

Query:   21 SAPSPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            S  +P+  LL+ L V G     ++DL  LGP+YG+LP+S+RVLLESAVRNCDNFQV E DV  IL W  NQ V  GVEVPF+PARVILQDFTGVPAVVDFAAMRDAV+ LGGDP+ INP CPSDLVIDHSVQVDF RT DAL KNEELEFERNKERFTFLKWGAKAF+NMLIVPPGSGIVHQVNLEYLARV FTD   +YPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQ ISM++P+V+GYK+ G++    TSTD+VLTITK+LRQ+GVVGKFVEF+GPGVA+LSIADRATI+NMCPEYGATVG+FPVD  S+ YL+QTNRDA K++A+E YL AVG++R++ D+ +DP FS+V+ELDL++VV  +SGPKRP D+VSV+DMK +F  CL+  +GF G+GL  + ++ T  F+  GKE+ I HG+V+IAAITSCTNTSNPSVMLGAGL+AKKAVE GL V  Y+KTSLSPGSGVVT+YL+ESGVIPYL+ LGFSIVG+GCMTCIGNSGPL + + +AI+K +LVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAGRVDIDFETE LG   DGK VFLRD+WPSR EI  VEK  VIP MF +VYS+I  G++ W  L AP+GKLYPWDQ STYIKSPPFF GMTKELP  + ++NA  LL L DSVTTDHISPAGSIARNSPAARFL S+G+TPREFNSYGSRRGND +MARGTFANIRLVN F+   GP+T+H PSNE +DVFDAAE YK+E   L+++ G+DYG GSSRDWAAKGPFL G++AV+AESYERIHRSNLVGMG+VPLQ+++G+NA SLGLT KE F++ IP+DL+P QT+ VQ    KSF V  RFDTE++L YYRHGGILNYMIRKM+A
Sbjct:    2 SGSNPYDKLLKSLSVGGV-TYNYYDLQGLGPKYGKLPYSVRVLLESAVRNCDNFQVKEKDVENILNWEVNQKVEGGVEVPFKPARVILQDFTGVPAVVDFAAMRDAVKSLGGDPDKINPICPSDLVIDHSVQVDFSRTNDALQKNEELEFERNKERFTFLKWGAKAFRNMLIVPPGSGIVHQVNLEYLARVVFTDGSTLYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQAISMLIPQVVGYKLVGELSQYATSTDLVLTITKNLRQLGVVGKFVEFFGPGVANLSIADRATISNMCPEYGATVGFFPVDVNSLQYLRQTNRDAVKVQAVEQYLKAVGLLRDFKDSGEDPVFSQVIELDLSTVVTCISGPKRPQDKVSVTDMKVEFAKCLTNKVGFNGFGLSPEVMSKTGKFIHEGKEYTIRHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLEVPAYVKTSLSPGSGVVTHYLRESGVIPYLNKLGFSIVGFGCMTCIGNSGPLPDYMVDAIEKNELVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGRVDIDFETEPLGVGVDGKQVFLRDVWPSRTEIHHVEKVTVIPGMFRDVYSRIENGSNSWRSLSAPDGKLYPWDQKSTYIKSPPFFAGMTKELPKAQPIKNARVLLLLGDSVTTDHISPAGSIARNSPAARFLASRGLTPREFNSYGSRRGNDDIMARGTFANIRLVNHFVSAPGPRTVHFPSNEELDVFDAAERYKQEGTPLILIVGKDYGSGSSRDWAAKGPFLLGIKAVIAESYERIHRSNLVGMGVVPLQFLNGQNAKSLGLTGKEVFSIDIPSDLRPLQTVSVQTDAGKSFDVTVRFDTEVDLLYYRHGGILNYMIRKMLA 892          

BLAST of aconitase vs. nr
Match: gi|939243158|ref|XP_014240876.1| (cytoplasmic aconitate hydratase-like isoform X1 [Cimex lectularius])

HSP 1 Score: 1350.5 bits (3494), Expect = 0.000e+0
Identity = 650/892 (72.87%), Postives = 757/892 (84.87%), Query Frame = 0

Query:   21 SAPSPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMIA 912
            +  +P+  LL+ L V G     ++DL  LGP+YG+LP+S+RVLLESAVRNCDNFQV E DV  IL W  NQ V  GVEVPF+PARVILQDFTGVPAVVDFAAMRDAV+ LGGDP+ INP CPSDLVIDHSVQVDF RT DAL KNEELEFERNKERFTFLKWGAKAF+NMLIVPPGSGIVHQVNLEYLARV FTD   +YPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQ ISM++P+V+GYK+ G++    TSTD+VLTITK+LRQ+GVVGKFVEF+GPGVA+LSIADRATI+NMCPEYGATVG+FPVD  S+ YL+QTNRDA K++A+E YL AVG++R++ D+ +DP FS+V+ELDL++VV  +SGPKRP D+VSV+DMK +F  CL+  +GF G+GL  + ++ T  F+  GKE+ I HG+V+IAAITSCTNTSNPSVMLGAGL+AKKAVE GL V  Y+KTSLSPGSGVVT+YL+ESGVIPYL+ LGFSIVG+GCMTCIGNSGPL + + +AI+K +LVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAGRVDIDFETE LG   DGK VFLRD+WPSR EI  VEK  VIP MF +VYS+I  G++ W  L AP+GKLYPWDQ STYIKSPPFF GMTKELP  + ++NA  LL L DSVTTDHISPAGSIARNSPAARFL S+G+TPREFNSYGSRRGND +MARGTFANIRLVN F+   GP+T+H PSNE +DVFDAAE YK+E   L+++ G+DYG GSSRDWAAKGPFL G++AV+AESYERIHRSNLVGMG+VPLQ+++G+NA SLGLT KE F++ IP+DL+P QT+ VQ    KSF V  RFDTE++L YYRHGGILNYMIRKM+A
Sbjct:   11 TGSNPYDKLLKSLSVGGV-TYNYYDLQGLGPKYGKLPYSVRVLLESAVRNCDNFQVKEKDVENILNWEVNQKVEGGVEVPFKPARVILQDFTGVPAVVDFAAMRDAVKSLGGDPDKINPICPSDLVIDHSVQVDFSRTNDALQKNEELEFERNKERFTFLKWGAKAFRNMLIVPPGSGIVHQVNLEYLARVVFTDGSTLYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQAISMLIPQVVGYKLVGELSQYATSTDLVLTITKNLRQLGVVGKFVEFFGPGVANLSIADRATISNMCPEYGATVGFFPVDVNSLQYLRQTNRDAVKVQAVEQYLKAVGLLRDFKDSGEDPVFSQVIELDLSTVVTCISGPKRPQDKVSVTDMKVEFAKCLTNKVGFNGFGLSPEVMSKTGKFIHEGKEYTIRHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLEVPAYVKTSLSPGSGVVTHYLRESGVIPYLNKLGFSIVGFGCMTCIGNSGPLPDYMVDAIEKNELVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGRVDIDFETEPLGVGVDGKQVFLRDVWPSRTEIHHVEKVTVIPGMFRDVYSRIENGSNSWRSLSAPDGKLYPWDQKSTYIKSPPFFAGMTKELPKAQPIKNARVLLLLGDSVTTDHISPAGSIARNSPAARFLASRGLTPREFNSYGSRRGNDDIMARGTFANIRLVNHFVSAPGPRTVHFPSNEELDVFDAAERYKQEGTPLILIVGKDYGSGSSRDWAAKGPFLLGIKAVIAESYERIHRSNLVGMGVVPLQFLNGQNAKSLGLTGKEVFSIDIPSDLRPLQTVSVQTDAGKSFDVTVRFDTEVDLLYYRHGGILNYMIRKMLA 901          

BLAST of aconitase vs. nr
Match: gi|242018544|ref|XP_002429734.1| (aconitase, putative [Pediculus humanus corporis] >gi|212514746|gb|EEB16996.1| aconitase, putative [Pediculus humanus corporis])

HSP 1 Score: 1347.8 bits (3487), Expect = 0.000e+0
Identity = 645/871 (74.05%), Postives = 748/871 (85.88%), Query Frame = 0

Query:   42 KFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMING-LGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            K+FDL + G  Y +LP+SIRVLLESAVRNCDNFQV E DV  IL+W  NQ VP GVEV F+PARVILQDFTGVPAVVDFAAMRDAV+ LGGDP+ INP CPSDLVIDHSVQVDFV+T DAL KNE+LEFERNKERFTFLKWGAKAF+NMLIVPPGSGIVHQVNLE+LAR+ F   +++YPDSVVGTDSHTTMING LGVVGWGVGGIEAEAVMLGQ ISM+LP+V+GYKI+G +   VTSTD+VLTITKHLRQ+GVVGKFVEF+GPGV++LSIADRATI+NMCPEYGATVG+FPVD +S+DYLK+TNR  +KIK +E YL    M+R+Y+D++QDP +S+V+ELDL +VV S+SGPKRPHDRVSVS+MKTDFLNCL+  +GFKG+GL  ++      F   GK++++ HG+V+IAAITSCTNTSNPSVMLGAGL+AKKAV+ GL+  PYIKTSLSPGSGVVTYYLKESGV+PYL+ LGFS+VGYGCMTCIGNSGPL   V EAI+K DLVCCGVLSGNRNFEGRIH +TRANYLASP LVIAY IAGRVDIDFETE LG   DGK ++LRDIWP+R EIQ VEKK+VIP MFEEVY KI  G+  W  ++AP  KLYPW++ STYIK PPFFDGMT+ LP  + V+NA  LLNL DSVTTDHISPAGSIARNSPAARFL SKG+ PR+FNSYGSRRGNDAVMARGTFANIRLVNKF+ Q GPKT+HIP+ E MDVFDAAE YK EN  L+ILAG+DYG GSSRDWAAKGP+L G++AV+AE+YERIHRSNLVGMGI+PLQY+ G++A+ L LT KE +T+ IP D+KP Q + V ++  +SF    R DTE+ELTYY++GGILNYMIRKM+
Sbjct:   22 KYFDLCAFGDAYQRLPYSIRVLLESAVRNCDNFQVTENDVKNILQWETNQKVPGGVEVAFKPARVILQDFTGVPAVVDFAAMRDAVKLLGGDPDKINPICPSDLVIDHSVQVDFVKTSDALQKNEDLEFERNKERFTFLKWGAKAFRNMLIVPPGSGIVHQVNLEHLARLVFNVDNLLYPDSVVGTDSHTTMINGRLGVVGWGVGGIEAEAVMLGQSISMLLPEVVGYKITGTLGPYVTSTDLVLTITKHLRQLGVVGKFVEFFGPGVSALSIADRATISNMCPEYGATVGFFPVDQESLDYLKKTNRPVSKIKVMEHYLKTTKMLRDYADSTQDPVYSQVIELDLGTVVSSVSGPKRPHDRVSVSEMKTDFLNCLTNKVGFKGFGLNAEQTKAMCSFNYEGKDYILKHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVQAGLTTLPYIKTSLSPGSGVVTYYLKESGVVPYLEQLGFSVVGYGCMTCIGNSGPLPPSVVEAIEKADLVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYTIAGRVDIDFETEPLGMTPDGKEIYLRDIWPTRAEIQEVEKKHVIPVMFEEVYGKIQNGSLSWQSIQAPPSKLYPWNETSTYIKKPPFFDGMTRALPGVKSVKNARVLLNLGDSVTTDHISPAGSIARNSPAARFLASKGLMPRDFNSYGSRRGNDAVMARGTFANIRLVNKFMNQPGPKTIHIPTGEEMDVFDAAERYKMENTPLIILAGKDYGSGSSRDWAAKGPYLLGIKAVIAETYERIHRSNLVGMGIIPLQYLEGQDAEKLKLTGKETYTIDIPDDVKPLQKITVNLNDGRSFDTRLRLDTEVELTYYKNGGILNYMIRKML 892          

BLAST of aconitase vs. nr
Match: gi|998504501|ref|XP_015512413.1| (PREDICTED: cytoplasmic aconitate hydratase-like [Neodiprion lecontei])

HSP 1 Score: 1345.49 bits (3481), Expect = 0.000e+0
Identity = 653/888 (73.54%), Postives = 755/888 (85.02%), Query Frame = 0

Query:   24 SPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            +P+  LL+ +++ G  + +++D+AS G +Y +LPFSIRVLLESAVRNCD FQV +TDV KILKW  NQ VP GVEV F+PARVILQDFTGVPAVVDFAAMRDAV+ LGGDP+ INP CPSDLVIDHSVQVDF R  D+L KNEELEFERNKERF FLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARV F +  ++YPDSVVGTDSHTTMINGLGV+GWGVGGIEAEAVMLGQ ISM+LPKV+GYK+ G ++  VTSTD+VLTITK+LRQ+GVVGKFVEF+GPGVA LSIADRATI+NMCPEYGATVG+F VD QS+ YL+QT+R    +  IE YL  V M+RNY+D SQDP FSEVV LDLA+VV S+SGPKRPHDRVSVSDMK DFL+CLSA IGFKG+GL  +KL T   F   GK++ ++HG+V+IAAITSCTNTSNPSVMLGAGL+AKKAVE GL+VAPYIKTSLSPGSGVVTYYLKESGVIP L  LGF +VGYGCMTCIGNSGPL + + +AI+K +LVCCGVLSGNRNFEGRIH +TRANYLASP LVIAYAIAG V IDF+TE LG+ ADGK +FLRDIWP+R EIQ VE+K+VIP MF EVYSKI  G+  W+ L+APEGKLYPWD  STYIK PP+F+G+TKELP  R +  A  LLNL DSVTTDHISPAGSIARNSPAAR+L  +G+ P+EFNSYGSRRGNDAVMARGTFANIRLVNKFIG+AGP+T++IP+NE MD+FDAAE+Y  +   L+ L G++YG GSSRDWAAKGPFLQG+RAV+AESYERIHRSNLVGMGI+PLQY+ G+ ADSLGLT  E + + IP D +P QT+ V     K F V+ RFDTE++LTY++HGGILNYMIR M+
Sbjct:    5 NPYKRLLKTIEIGGI-DCQYYDVASFGDKYDKLPFSIRVLLESAVRNCDEFQVKKTDVEKILKWETNQKVPEGVEVAFKPARVILQDFTGVPAVVDFAAMRDAVKSLGGDPDKINPICPSDLVIDHSVQVDFTRGDDSLKKNEELEFERNKERFMFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVVFNNDGVLYPDSVVGTDSHTTMINGLGVLGWGVGGIEAEAVMLGQAISMLLPKVVGYKLEGILNQYVTSTDLVLTITKNLRQLGVVGKFVEFFGPGVAELSIADRATISNMCPEYGATVGFFAVDQQSLSYLRQTSRSEKHVTYIEEYLRTVKMLRNYNDPSQDPVFSEVVTLDLATVVSSVSGPKRPHDRVSVSDMKNDFLSCLSAKIGFKGFGLKPEKLKTEGKFQFEGKDYTLNHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLTVAPYIKTSLSPGSGVVTYYLKESGVIPSLMQLGFDVVGYGCMTCIGNSGPLPDSMVDAIEKNELVCCGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGTVIIDFDTEPLGRRADGKEIFLRDIWPTRAEIQTVEQKHVIPAMFTEVYSKIEKGSGSWSSLQAPEGKLYPWDAESTYIKHPPYFEGLTKELPPARPIRRARVLLNLGDSVTTDHISPAGSIARNSPAARYLAKRGLKPKEFNSYGSRRGNDAVMARGTFANIRLVNKFIGKAGPRTIYIPTNEEMDIFDAAEKYVNDGVPLIALVGKEYGSGSSRDWAAKGPFLQGIRAVIAESYERIHRSNLVGMGIIPLQYLPGQTADSLGLTGLELYDIIIPTDCRPGQTITVTTDDGKRFDVIVRFDTEVDLTYFKHGGILNYMIRTMV 891          

BLAST of aconitase vs. nr
Match: gi|665796130|ref|XP_008545838.1| (PREDICTED: cytoplasmic aconitate hydratase [Microplitis demolitor])

HSP 1 Score: 1343.56 bits (3476), Expect = 0.000e+0
Identity = 634/891 (71.16%), Postives = 745/891 (83.61%), Query Frame = 0

Query:   21 SAPSPFGHLLRDLKVEGHGNLKFFDLASLGPEYGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            + P+P+ +LL+ + +       ++D++S G +Y +LPFSI++LLESAVRNCD FQV ++DV KIL W  NQ+    VEV F+PARVILQD TGVPAVVDFAAMRD V+RL GDP  INP CPSDLVIDHS+QVDFVR+ DAL KNE+LEFERNKERF FLKWGAKA KNMLIVPPGSGIVHQVNLEYLARV F   D++YPDSVVGTDSHTTMINGLG++GWGVGGIEAEAVMLGQ +SM+LPKV+GYK+ G ++  VTSTD+VLTITK+LRQ+GVVGKFVEFYGPGVA LSIADRATI+NMCPEYGATVG+FPVD QS++YL+QT R    I  IE YL AV M+R+Y+  SQDP FSE V LDLA+VV S+SGPKRPHDRVSVSDMK DF  CL+  IGFKGYGL  DK+ T   F+  G+E+ + HG+V+IAAITSCTNTSNPSVMLGAGL+AK AV+ GL+VAPYIKTSLSPGSGVVTYYL+ESGVIP L  LGF IVGYGCMTCIGNSGPLE+ ++E I+K +LVCCGVLSGNRNFEGRIH  TRANYLASP LVIAYAIAG VDIDFETE LG+ ADGK ++LRDIWP+R +IQAVE+KYVIP MF+ VY KI  G++RW  L APEG+LYPW+++STYIK PP+F+ + KELP  + +  A  LLNL DSVTTDHISPAGSIARNSPAAR+L  +G+TPREFNSYG+RRGNDAVM RGTFANIRL+NKF+G+ GPKTL++P+NE MD+FDAAE Y K+   L+ L G++YG GSSRDWAAKGP+L G+RA++AESYERIHRSNLVGMGIVPLQY+ G+ AD+LGLT  E F + IPAD +P Q + V+    K F V+ RFDTE++LTY+RHGGILNYMIR MI
Sbjct:    2 AVPNPYDNLLKSVVINSK-ERNYYDVSSFGKKYDRLPFSIKILLESAVRNCDGFQVKDSDVKKILDWEINQA-SDDVEVAFKPARVILQDLTGVPAVVDFAAMRDCVKRLNGDPNKINPICPSDLVIDHSIQVDFVRSGDALKKNEDLEFERNKERFIFLKWGAKALKNMLIVPPGSGIVHQVNLEYLARVVFDSDDLLYPDSVVGTDSHTTMINGLGILGWGVGGIEAEAVMLGQAVSMLLPKVIGYKLVGTLNQYVTSTDLVLTITKNLRQLGVVGKFVEFYGPGVAELSIADRATISNMCPEYGATVGFFPVDQQSLNYLRQTGRSEEHIDRIEKYLRAVKMLRDYNIESQDPVFSETVTLDLATVVSSVSGPKRPHDRVSVSDMKLDFNQCLTNKIGFKGYGLSADKIKTEGKFMFEGQEYTLKHGSVVIAAITSCTNTSNPSVMLGAGLLAKNAVDAGLTVAPYIKTSLSPGSGVVTYYLRESGVIPALTQLGFDIVGYGCMTCIGNSGPLEDSIAETIEKKELVCCGVLSGNRNFEGRIHPSTRANYLASPLLVIAYAIAGTVDIDFETEPLGRRADGKDIYLRDIWPTRADIQAVEQKYVIPAMFKSVYDKIEKGSNRWASLVAPEGQLYPWNEDSTYIKHPPYFETLEKELPEIKPIRKARVLLNLGDSVTTDHISPAGSIARNSPAARYLAKRGLTPREFNSYGARRGNDAVMVRGTFANIRLLNKFVGKPGPKTLYLPTNEEMDIFDAAERYGKDKVPLIALVGKEYGSGSSRDWAAKGPYLLGIRAIIAESYERIHRSNLVGMGIVPLQYLPGQTADTLGLTGVELFDIDIPADCQPHQQITVRTDTGKEFTVIVRFDTEVDLTYFRHGGILNYMIRTMI 890          

BLAST of aconitase vs. nr
Match: gi|676423145|ref|XP_009044065.1| (hypothetical protein LOTGIDRAFT_181349 [Lottia gigantea] >gi|556116868|gb|ESP05520.1| hypothetical protein LOTGIDRAFT_181349 [Lottia gigantea])

HSP 1 Score: 1338.94 bits (3464), Expect = 0.000e+0
Identity = 649/894 (72.60%), Postives = 751/894 (84.00%), Query Frame = 0

Query:   19 APSAPSPFGHLLRDLKVEGHGNLKFFDLASLGPE-YGQLPFSIRVLLESAVRNCDNFQVLETDVIKILKWAQNQSVPSGVEVPFRPARVILQDFTGVPAVVDFAAMRDAVQRLGGDPEMINPQCPSDLVIDHSVQVDFVRTPDALSKNEELEFERNKERFTFLKWGAKAFKNMLIVPPGSGIVHQVNLEYLARVCFTDSDIVYPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPKVLGYKISGKMHSLVTSTDVVLTITKHLRQIGVVGKFVEFYGPGVASLSIADRATIANMCPEYGATVGYFPVDSQSIDYLKQTNRDATKIKAIEAYLNAVGMMRNYSDASQDPTFSEVVELDLASVVPSLSGPKRPHDRVSVSDMKTDFLNCLSANIGFKGYGLPKDKLTTTVPFVLNGKEHVISHGAVLIAAITSCTNTSNPSVMLGAGLMAKKAVEKGLSVAPYIKTSLSPGSGVVTYYLKESGVIPYLDALGFSIVGYGCMTCIGNSGPLEEPVSEAIKKGDLVCCGVLSGNRNFEGRIHADTRANYLASPPLVIAYAIAGRVDIDFETEALGQDADGKPVFLRDIWPSREEIQAVEKKYVIPTMFEEVYSKITTGNDRWNKLEAPEGKLYPWDQNSTYIKSPPFFDGMTKELPAPRKVENALPLLNLADSVTTDHISPAGSIARNSPAARFLGSKGITPREFNSYGSRRGNDAVMARGTFANIRLVNKFIGQAGPKTLHIPSNEVMDVFDAAEEYKKENRHLVILAGQDYGCGSSRDWAAKGPFLQGVRAVMAESYERIHRSNLVGMGIVPLQYMSGENADSLGLTAKEEFTVHIPADLKPQQTLEVQVSGKKSFKVLARFDTELELTYYRHGGILNYMIRKMI 911
            A     PF  LL+    EG  + KFFDLA L  E Y +LPFSIRVLLESAVRNCD F + ++DV  IL W++ Q     VE+PF+PARVILQDFTGVPAVVDFAAMRDAV+++GGDPE INP CP+DLVIDHSVQVD  +T DAL KN+ELEF+RNKERF FLKWGAKA KNMLIVPPGSGIVHQVNLEYLARV F  +  +YPDS+VGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQ ISMVLP+V+GYK+ G   S +TSTD+VLT+TKHLRQIGVVGKFVEF+GPGV SLSIADRATI+NMCPEYGAT+G+FPVD  SI YLKQT RD  K+  IE YL    M R+YS+  +DP FS++VELDL SVV   SGPKRPHD+V VSDMKTDF NCL   + FKG+ +PKDK +T VP V + KE++++HG+V+IAAITSCTNTSNPSVMLGAGL+A+ AVE GL+++PYIKTSLSPGSGVVTYYL+ESGVIPYL+ LGF +VGYGCMTCIGNSGPL EPV+E I+KGDLVC GVLSGNRNFEGRIH  TRANYLASPPLVIAYAIAG V+IDFETE LG  ADG PVFLRDIWP RE+I  VEK++V+P+MF++VYSKIT+GN RWN LEAPE  LYPWD+ STYIKSPPFF+ MTKE+  P  +ENA  LLNL DSVTTDHISPAGSIARNSPAAR+L  +G+ PREFNSYGSRRGNDAVMARGTFANIRL+NKFIG+ G +T+++P+ + MDVFDAAE Y+KEN+ ++ILAG++YG GSSRDWAAKGP++QGV+AV+AESYERIHRSNLVGMGIVP QY+ G+NA+SLGLT KE F+V +P D+K  Q L V V   KSF V  RFDTE+EL Y++HGGILNYMIR M+
Sbjct:    2 ASDGTHPFASLLKSQSFEGK-DYKFFDLAGLKDERYDKLPFSIRVLLESAVRNCDEFVIRKSDVENILNWSKTQH--EQVEIPFKPARVILQDFTGVPAVVDFAAMRDAVKKMGGDPEKINPICPADLVIDHSVQVDVSKTSDALEKNQELEFQRNKERFLFLKWGAKALKNMLIVPPGSGIVHQVNLEYLARVVFDSNGTLYPDSLVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQSISMVLPEVIGYKLCGTPGSYITSTDIVLTVTKHLRQIGVVGKFVEFFGPGVNSLSIADRATISNMCPEYGATIGFFPVDDASISYLKQTGRDDEKLILIEKYLQLNKMFRDYSNPEEDPVFSQIVELDLGSVVSCCSGPKRPHDKVPVSDMKTDFQNCLDNKVSFKGFAIPKDKQSTNVPIVYDNKEYILNHGSVVIAAITSCTNTSNPSVMLGAGLLARNAVEAGLTISPYIKTSLSPGSGVVTYYLRESGVIPYLEKLGFDVVGYGCMTCIGNSGPLPEPVAEGIEKGDLVCVGVLSGNRNFEGRIHPLTRANYLASPPLVIAYAIAGTVNIDFETEPLGTRADGTPVFLRDIWPKREDIVTVEKQFVVPSMFKDVYSKITSGNKRWNALEAPESMLYPWDEKSTYIKSPPFFEKMTKEIQPPSSIENAYVLLNLGDSVTTDHISPAGSIARNSPAARYLAGRGLAPREFNSYGSRRGNDAVMARGTFANIRLLNKFIGKPGARTVYVPTGQEMDVFDAAERYQKENKQVIILAGKEYGSGSSRDWAAKGPWMQGVKAVIAESYERIHRSNLVGMGIVPFQYLDGQNAESLGLTGKETFSVSVPEDVKAGQILTVNVKDGKSFDVKTRFDTEVELAYFKHGGILNYMIRGML 892