BLAST of vasa intronic gene vs. L. salmonis genes
Match: EMLSAG00000012946 (supercontig:LSalAtl2s:LSalAtl2s989:10662:14348:1 gene:EMLSAG00000012946 transcript:EMLSAT00000012946 description:"augustus_masked-LSalAtl2s989-processed-gene-0.1")

HSP 1 Score: 149.058 bits (375), Expect = 1.128e-41
Identity = 98/167 (58.68%), Postives = 124/167 (74.25%), Query Frame = 0

Query:  242 GRGGPGGRGGKRDFDRKSGDDRTSGVKHVDKREGGGSHNWGTFEDEIKAVDDKNNVSTEENP-AEAGVERSGDDNAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEKESAMDDEDEEDQELYPQRVNRQRRVLDIEFNFTT 407
            GRG P     KR+F+RKSGDD+T GVK VDKR+GGGSHNWGT EDE+KA +DK NVS  E+P  +    R G D  +D+   + + KT+TL+EW+A Q  K+  P +N RKAGEG +  +  WKKTYAYKKEKE+  DD++E+D ELYPQRVNRQ+R+LDIEFNFT+
Sbjct:   52 GRGKP-----KREFERKSGDDKT-GVKAVDKRDGGGSHNWGTMEDEMKAEEDKANVSASEDPNNQEESARPGSD-EDDEEANKNKVKTLTLEEWKAQQKEKREVPTYNTRKAGEG-SEIDPKWKKTYAYKKEKETH-DDDEEDDHELYPQRVNRQKRILDIEFNFTS 209          

BLAST of vasa intronic gene vs. SwissProt
Match: gi|52783213|sp|Q9CY58.2|PAIRB_MOUSE (RecName: Full=Plasminogen activator inhibitor 1 RNA-binding protein; AltName: Full=PAI1 RNA-binding protein 1; Short=PAI-RBP1; AltName: Full=SERPINE1 mRNA-binding protein 1)

HSP 1 Score: 85.5001 bits (210), Expect = 5.247e-17
Identity = 68/174 (39.08%), Postives = 89/174 (51.15%), Query Frame = 0

Query:  251 GKRDFDRKSGDDRTS-----GVKHVDKREGGGSHNWGTFEDEI--------KAVD------DKNNVSTEENP--AEAGVERSGDDNAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEKESAMDDEDEEDQELYPQRVNRQRRVLDIEF 403
            GKR+FDR SG DR+S     G+KH DKR G GSHNWGT +DE+        K +       D++NV TEE P   E  V  + +   E +  +EE PK MTLDEW+A Q+  +    FN+RK  EGA   +  WKK +   K K      ED      + +  N     L+I F
Sbjct:  189 GKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNCSDLDQSNV-TEETPEGEEHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGA---DGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINF 358          

BLAST of vasa intronic gene vs. SwissProt
Match: gi|52783155|sp|Q6AXS5.2|PAIRB_RAT (RecName: Full=Plasminogen activator inhibitor 1 RNA-binding protein; AltName: Full=PAI1 RNA-binding protein 1; Short=PAI-RBP1; AltName: Full=RDA288; AltName: Full=SERPINE1 mRNA-binding protein 1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.399e-16
Identity = 65/173 (37.57%), Postives = 85/173 (49.13%), Query Frame = 0

Query:  251 GKRDFDRKSGDDRTS-----GVKHVDKREGGGSHNWGTFEDEIK-------------AVDDKNNVSTEENP--AEAGVERSGDDNAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEKESAMDDEDEEDQELYPQRVNRQRRVLDIEF 403
            GKR+FDR SG DR+S     G+KH DKR G GSHNWGT +DE+                D + +  TEE P   E  V  + +   E +  +EE PK MTLDEW+A Q+  +    FN+RK  EGA   +  WKK +   K K      ED      + +  N     L+I F
Sbjct:  189 GKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNCSDLEQSNVTEETPEGEEHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGA---DGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINF 358          

BLAST of vasa intronic gene vs. SwissProt
Match: gi|52783206|sp|Q8NC51.2|PAIRB_HUMAN (RecName: Full=Plasminogen activator inhibitor 1 RNA-binding protein; AltName: Full=PAI1 RNA-binding protein 1; Short=PAI-RBP1; AltName: Full=SERPINE1 mRNA-binding protein 1)

HSP 1 Score: 83.5741 bits (205), Expect = 2.588e-16
Identity = 67/175 (38.29%), Postives = 88/175 (50.29%), Query Frame = 0

Query:  251 GKRDFDRKSGDDRTS-----GVKHVDKREGGGSHNWGTFEDEI--------KAVD------DKNNVSTEENP---AEAGVERSGDDNAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEKESAMDDEDEEDQELYPQRVNRQRRVLDIEF 403
            GKR+FDR SG DR+S     G+KH DKR G GSHNWGT +DE+        K +       D++NV TEE P       V  + +   E +  +EE PK MTLDEW+A Q+  +    FN+RK  EGA   +  WKK +   K K      ED      + +  N     L+I F
Sbjct:  189 GKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNYSDLDQSNV-TEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGA---DGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINF 359          

BLAST of vasa intronic gene vs. nr
Match: gi|914577589|gb|KOB79256.1| (Vasa intronic protein [Operophtera brumata])

HSP 1 Score: 151.369 bits (381), Expect = 4.470e-38
Identity = 140/384 (36.46%), Postives = 183/384 (47.66%), Query Frame = 0

Query:   65 MESTYGVGINNRYALFLDEEGEESEQALLEASLAAQKIVDNNLKAQAAAAAVPVVT---------KGPKDAKPAATTDPK-------KAPAHMNPKNNNLNHRRDDKENRRFEGKRVLDARKAPDAPRENNEERTNRRNNRDAPDGERRNQFDGE----------------RRGPYDSSEGGRGRGRGSRGAPGTGRGMERGGPRGNGGGRGGPGGRGGKRDFDRKSGDDRTSGVKHVDKREGGGSHNWGTFEDEIKAVDDKNNVSTE-----ENPAEAGVERSGDDNAED--QIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEK----ESAMDDEDEEDQELYPQRVNRQRRVLDIEFNF 405
            ME++YGVG+ NRYALFLD+E +  +   ++AS  A+++      A+      P V          KG K+ +   + D K       K PA    +NN            R +   V     AP  PR +  ER   R     P+ ERRN FD                   R P DS +G RG                   PR        P    GKR+FDR+SG D+T GVK VDKREGGG HNWGT +D+I   DD N   +E     E PA      +GD  A +    P EEEP+ +TLDE++A ++A++  P++NLRKAGEG   T+  WKK    +K+K    E   D +DE D   YPQRV RQ+RVL IEF F
Sbjct:    1 MENSYGVGVVNRYALFLDDESDPLD--AIKASEQAKELKKKTKDAEKENKGKPEVKPKGPTAPARKGIKETQNVKSQDIKSGEQQKAKGPARPVDRNNAERPPPRRPREDRPQNGAVDSKDGAPRPPRRDFAERGGERR----PNFERRN-FDNPEGGERRGPRPPREPRAERDPRDSRDGPRG-------------------PRP-------PYDNRGKREFDRRSGSDKT-GVKPVDKREGGGPHNWGTIKDDI---DDLNKTGSEGEPVEEKPAAEASVGAGDGVAPEPPTAPAEEEPRELTLDEYKALRNAQRTQPQYNLRKAGEGEDLTQ--WKKLVPLEKKKEVTGEEGEDTDDEYDLADYPQRVGRQKRVLGIEFTF 345          

BLAST of vasa intronic gene vs. nr
Match: gi|1059387694|ref|XP_017769048.1| (PREDICTED: plasminogen activator inhibitor 1 RNA-binding protein-like, partial [Nicrophorus vespilloides])

HSP 1 Score: 141.354 bits (355), Expect = 1.011e-34
Identity = 132/364 (36.26%), Postives = 188/364 (51.65%), Query Frame = 0

Query:   65 MESTYGVGINNRYALFLDEEGEESEQALL---EASLAAQ-KIVDNNLKAQAAAAAVPVVTKGPKDAKPAATTDPKKAPAHMNPKNNNLNHRR--DDKENRRF------EGKRVLDARKAPDAPRENNEERTNRRNNR-DAPDGERRNQFDGERRGPYDSSEGGRGRGRGSRGAPGTGRGMERGGPRGNGGGRGGPGGRGGKRDFDRKSGDDRTSGVKHVDKREGGGSHNWGTFEDEIKAVDDKNNVSTEE-----NPAEAGV--ERSGDDNAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEKESAMDDEDEEDQEL--YPQRVNRQRRVLDIEFNFT 406
            ME+ YG+G  NRY+LFLD E +  E   +   E  L  + K+V+   K +  A A    T+ PK         P KA  +   ++  LNH+R  D+K  + F        +   + R   D P   N E     NNR D P           R G +D+    RGR  GS+ A                 GRGG   R GKR+F+R+SG D+T GVK +DKR+GGG+HNWG+ +D I+  D   +  TE+       AE  +  E+   +N  + +P EEEPK +TLDEW+A Q A +  P +NLRKAGEG   ++  WKK Y   K+K+   ++ D+E+ +   YPQRV RQ+RV+ I+F+F+
Sbjct:    1 MENVYGIGTANRYSLFLDNETDPLETLQIKEQEKELKRKTKVVEKENKGKPEAPAKGKPTQPPKKIIKDV---PHKAQDNKR-EDGKLNHQRSTDNKTEKTFNKFNNENREERNNRRNREDRPFNGNFE-----NNREDRP----------RREGDFDNR---RGRAVGSKTA-----------------GRGGVAPRRGKREFERQSGSDKT-GVKPIDKRDGGGAHNWGSHKDVIEEADANKSTETEQVWSENEKAETSINAEQKETENETENVPVEEEPKELTLDEWKA-QRAGRQKPTYNLRKAGEGEDPSQ--WKKMYELSKKKDGEEEETDDEEYDTSEYPQRVGRQKRVVGIDFHFS 321          

BLAST of vasa intronic gene vs. nr
Match: gi|1344765879|ref|XP_023935179.1| (plasminogen activator inhibitor 1 RNA-binding protein-like [Bicyclus anynana] >gi|1063195365|gb|AON96563.1| plasminogen activator inhibitor 1 RNA-binding protein-like protein [Bicyclus anynana])

HSP 1 Score: 142.124 bits (357), Expect = 1.063e-34
Identity = 131/361 (36.29%), Postives = 180/361 (49.86%), Query Frame = 0

Query:   65 MESTYGVGINNRYALFLDEEGE--------ESEQALLEASLAAQKIVDNNLKAQAAAAAVPVVTKGPKDAKPAATTDPKKAPAHMN---PKNNNLNHRRDDKENRRFEGKR-VLDARKAPDAPRENNEERTNRRNNRDAPDGERRNQFDGERRGPYDSSEGGRGRGRGSRGAPGTGRGMERGGPRGNGGGRGGPGGRGGKRDFDRKSGDDRTSGVKHVDKREGGGSHNWGTFEDEIKAVDDKNNVSTE-----ENPAEAGVERSGDD-NAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAY--KKEKESAMDDEDEEDQELYPQRVNRQRRVLDIEFNF 405
            ME++YGVG+ NRYALFLD+E +        E  + L + +  A+K     L+ +    +V VV KG K+ +   + + K      N   P+  + N  R     R    +   ++ ++    PR    E  +RR     P+ ERRN  D    G        R    G RG            PR    GRG       KR+FDR+SG D+T GVK VDKREG G HNWGT +D++   D+ N   +E     E P +A     G     E   P EEEP+ +TLDE++A ++A++ AP++NLRKAGEG   ++  WK       KKE E   D++DE D   YPQRV RQ+RVL IEF F
Sbjct:    1 MENSYGVGVVNRYALFLDDESDPLDAIKAREQAKELKKKTKDAEKENKGKLEVKPKGPSV-VVRKGIKETQNVKSQENKTGDQQKNKGPPRTTDRNAERPPPRRREERPQNGAVEGKEGARPPRR---EFGDRR-----PNFERRNFNDNPEGGERRGPRPPREPRDGQRG------------PRPGYDGRG-------KREFDRRSGSDKT-GVKPVDKREGAGPHNWGTLKDDL---DEMNKTGSEGEAPEEKPVDAAAAGDGPQPEPERAPPAEEEPRELTLDEYKALRNAQRTAPQYNLRKAGEGEDLSQ--WKNLVQMEKKKEGEHDSDEDDEYDIADYPQRVGRQKRVLGIEFTF 327          

BLAST of vasa intronic gene vs. nr
Match: gi|1059402615|ref|XP_017774220.1| (PREDICTED: plasminogen activator inhibitor 1 RNA-binding protein-like isoform X2 [Nicrophorus vespilloides])

HSP 1 Score: 141.354 bits (355), Expect = 3.760e-34
Identity = 132/364 (36.26%), Postives = 188/364 (51.65%), Query Frame = 0

Query:   65 MESTYGVGINNRYALFLDEEGEESEQALL---EASLAAQ-KIVDNNLKAQAAAAAVPVVTKGPKDAKPAATTDPKKAPAHMNPKNNNLNHRR--DDKENRRF------EGKRVLDARKAPDAPRENNEERTNRRNNR-DAPDGERRNQFDGERRGPYDSSEGGRGRGRGSRGAPGTGRGMERGGPRGNGGGRGGPGGRGGKRDFDRKSGDDRTSGVKHVDKREGGGSHNWGTFEDEIKAVDDKNNVSTEE-----NPAEAGV--ERSGDDNAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEKESAMDDEDEEDQEL--YPQRVNRQRRVLDIEFNFT 406
            ME+ YG+G  NRY+LFLD E +  E   +   E  L  + K+V+   K +  A A    T+ PK         P KA  +   ++  LNH+R  D+K  + F        +   + R   D P   N E     NNR D P           R G +D+    RGR  GS+ A                 GRGG   R GKR+F+R+SG D+T GVK +DKR+GGG+HNWG+ +D I+  D   +  TE+       AE  +  E+   +N  + +P EEEPK +TLDEW+A Q A +  P +NLRKAGEG   ++  WKK Y   K+K+   ++ D+E+ +   YPQRV RQ+RV+ I+F+F+
Sbjct:    1 MENVYGIGTANRYSLFLDNETDPLETLQIKEQEKELKRKTKVVEKENKGKPEAPAKGKPTQPPKKIIKDV---PHKAQDNKR-EDGKLNHQRSTDNKTEKTFNKFNNENREERNNRRNREDRPFNGNFE-----NNREDRP----------RREGDFDNR---RGRAVGSKTA-----------------GRGGVAPRRGKREFERQSGSDKT-GVKPIDKRDGGGAHNWGSHKDVIEEADANKSTETEQVWSENEKAETSINAEQKETENETENVPVEEEPKELTLDEWKA-QRAGRQKPTYNLRKAGEGEDPSQ--WKKMYELSKKKDGEEEETDDEEYDTSEYPQRVGRQKRVVGIDFHFS 321          

BLAST of vasa intronic gene vs. nr
Match: gi|1048010958|ref|XP_017466331.1| (PREDICTED: plasminogen activator inhibitor 1 RNA-binding protein [Rhagoletis zephyria])

HSP 1 Score: 141.354 bits (355), Expect = 4.479e-34
Identity = 81/161 (50.31%), Postives = 107/161 (66.46%), Query Frame = 0

Query:  247 GGRGGKRDFDRKSGDDRTSGVKHVDKREGGGSHNWGTFEDEIKAVDDKNNVSTEENPAEAGVERSGDDNAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAY--KKEKESAMDDEDEEDQELYPQRVNRQRRVLDIEFNF 405
            G RGGKR+FDR+SG D+T GVK +DKR+G G+HNWG+ ++   A+DD N    E        E SG++ A DQ P EEE K +TLDEW+A Q  ++  P FN+RKAGEG   T+  WKK       K+K++  ++E E D  +YPQRV RQ+RVLDI+FNF
Sbjct:  191 GNRGGKREFDRQSGSDKT-GVKAIDKRDGAGAHNWGSVKE---AIDDVNKSDVEAGNVTDKAEESGNEQA-DQAPVEEETKELTLDEWKA-QKQQRVKPTFNIRKAGEGEDTTQ--WKKMVVLNNNKKKDNESEEELEYDPAMYPQRVGRQQRVLDIQFNF 343          

BLAST of vasa intronic gene vs. nr
Match: gi|1059402613|ref|XP_017774219.1| (PREDICTED: plasminogen activator inhibitor 1 RNA-binding protein-like isoform X1 [Nicrophorus vespilloides])

HSP 1 Score: 140.584 bits (353), Expect = 8.142e-34
Identity = 132/367 (35.97%), Postives = 188/367 (51.23%), Query Frame = 0

Query:   65 MESTYGVGINNRYALFLDEEGEESEQALL---EASLAAQ-KIVDNNLKAQAAAAAVPVVTKGPKDAKPAATTDPKKAPAHMNPKNNNLNHRR--DDKENRRF------EGKRVLDARKAPDAPRENNEERTNRRNNR-DAPDGERRNQFDGERRGPYDSSEGGRGRGRGSRGAPGTGRGMERGGPRGNGGGRGGPGGRGGKRDFDRKSGDDRTSG---VKHVDKREGGGSHNWGTFEDEIKAVDDKNNVSTEE-----NPAEAGV--ERSGDDNAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEKESAMDDEDEEDQEL--YPQRVNRQRRVLDIEFNFT 406
            ME+ YG+G  NRY+LFLD E +  E   +   E  L  + K+V+   K +  A A    T+ PK         P KA  +   ++  LNH+R  D+K  + F        +   + R   D P   N E     NNR D P           R G +D+    RGR  GS+ A                 GRGG   R GKR+F+R+SG D+T G   VK +DKR+GGG+HNWG+ +D I+  D   +  TE+       AE  +  E+   +N  + +P EEEPK +TLDEW+A Q A +  P +NLRKAGEG   ++  WKK Y   K+K+   ++ D+E+ +   YPQRV RQ+RV+ I+F+F+
Sbjct:    1 MENVYGIGTANRYSLFLDNETDPLETLQIKEQEKELKRKTKVVEKENKGKPEAPAKGKPTQPPKKIIKDV---PHKAQDNKR-EDGKLNHQRSTDNKTEKTFNKFNNENREERNNRRNREDRPFNGNFE-----NNREDRP----------RREGDFDNR---RGRAVGSKTA-----------------GRGGVAPRRGKREFERQSGSDKTHGYRGVKPIDKRDGGGAHNWGSHKDVIEEADANKSTETEQVWSENEKAETSINAEQKETENETENVPVEEEPKELTLDEWKA-QRAGRQKPTYNLRKAGEGEDPSQ--WKKMYELSKKKDGEEEETDDEEYDTSEYPQRVGRQKRVVGIDFHFS 325          

BLAST of vasa intronic gene vs. nr
Match: gi|1036945647|ref|XP_017005293.1| (PREDICTED: plasminogen activator inhibitor 1 RNA-binding protein [Drosophila takahashii])

HSP 1 Score: 139.043 bits (349), Expect = 3.955e-33
Identity = 87/183 (47.54%), Postives = 116/183 (63.39%), Query Frame = 0

Query:  233 RGGPRGNGGGRGGPGGRGGKRDFDRKSGDDRTSGVKHVDKREGGGSHNWGTFEDEIKAVDDKNNVSTEENPAEAGVERSGDDNAEDQIPREE-EPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEKESAMDDEDEEDQELYPQRVNRQRRVLDIEFNFTTEQGSRGG 414
            RG PR   GG      + GKR+FDR+SG D+T GVK +DKREGGG+HNWG+ + +I   +D      E +P     + + + +A+  +P EE E K MTLDEW+A +D ++  P +NLRKAGEGAA   A WKK     K+KES  +DE E D  LYPQRV R +R++DI+FNF    G +GG
Sbjct:  145 RGPPRARTGGE-----KFGKREFDRQSGSDKT-GVKSIDKREGGGAHNWGSPKQDI---EDLKTTGGESSPQAEKEDSANEQSADPAVPAEEDESKLMTLDEWKALRD-QRAKPNYNLRKAGEGAADN-AEWKKMVVLSKKKESNSEDELEYDPSLYPQRVGRLQRIVDIQFNFN--DGRKGG 314          

BLAST of vasa intronic gene vs. nr
Match: gi|1321321919|ref|XP_023303429.1| (plasminogen activator inhibitor 1 RNA-binding protein [Lucilia cuprina] >gi|906472339|gb|KNC32489.1| hypothetical protein FF38_00488 [Lucilia cuprina])

HSP 1 Score: 138.272 bits (347), Expect = 8.206e-33
Identity = 90/184 (48.91%), Postives = 118/184 (64.13%), Query Frame = 0

Query:  233 RGGPRGNGGGRGGPGGRGG--KRDFDRKSGDDRTSGVKHVDKREGGGSHNWGTFEDEIKAVDDKN-NVSTEENPAEAGV--ERSGDDNAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTY------AYKKEKESAMDDEDEEDQELYPQRVNRQRRVLDIEFNF 405
            RGG R +   R      GG  KR+FDR+SG D+T GVK VDKREG G+HNWG+ +D I+  ++ N NV    N A AG   E SG++ A +Q P EEE K +TLDEW+A Q  ++  P+FN+RKAGEG   T+  WKK        A K++K +  ++E E D  +YPQRV RQ+RVLDI+FNF
Sbjct:  173 RGGDRADRPPRSNRNFEGGNRKREFDRQSGSDKT-GVKAVDKREGAGAHNWGSVKDTIEEGNNTNKNVEENGNAANAGEKNEESGNEQATEQTPVEEEVKELTLDEWKAQQ-QQRAKPQFNIRKAGEGEDTTK--WKKMIVLNNSKATKEKKGTESEEELEFDPAMYPQRVGRQQRVLDIQFNF 352          

BLAST of vasa intronic gene vs. nr
Match: gi|914096868|ref|NP_001298538.1| (plasminogen activator inhibitor 1 RNA-binding protein-like [Papilio polytes] >gi|389610949|dbj|BAM19085.1| vasa intronic gene [Papilio polytes])

HSP 1 Score: 137.117 bits (344), Expect = 8.890e-33
Identity = 135/364 (37.09%), Postives = 182/364 (50.00%), Query Frame = 0

Query:   65 MESTYGVGINNRYALFLDEEGE------ESEQALLEASLAAQKIVDNNLKAQAAAAAVPVVT-KGPKDAKPAATTDPK-------KAPAHM--NPKNNNLNHRRDDK-ENRRFEGKRVLDARKAPDAPRENNEERTNRRNNRDAPDGERRNQFDGERRGPYDSSEGGRGRGRGSRGAPGTGRGMERGGPRGNGGGRGGPGGRGGKRDFDRKSGDDRTSGVKHVDKREGGGSHNWGTFED---EIKAVDDKNNVSTEENPAEAGVERSGDDNAEDQIPREEEPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEKESAMDDEDEEDQEL--YPQRVNRQRRVLDIEFNFT 406
            ME++YGVGI NRYALFLD+E +        EQA        +   +N  K +    +  VV  KG K+ +   + + K       K PA     P     + RR+++ +N   EGK V     AP  PR    ER         P  ERRN  D    G        R    G RG            PR     RG       KR+FDR+SG D+T GVK VDKREGGG HNWGT +D   E++    +     E+ P  A        +AE  +P EEEP+ +TLDE++A ++A++ AP++NLRKAGEG   ++  WK     +K+KE   DDE +E+ ++  YPQRV RQ+RVL IEF F+
Sbjct:    1 MENSYGVGIVNRYALFLDDETDPLDALKAREQAKELKKKTKEAEKENKGKPEIKPKSGSVVARKGIKETQNVKSQENKNGDQQKSKGPARTAERPVERPPSKRREERPQNGAVEGKEV-----APRPPRREFGERR--------PYIERRNFSDNPEGGERRGPRPPREPREGQRG------------PRPAYDSRG-------KREFDRRSGSDKT-GVKPVDKREGGGPHNWGTIKDDLDELQKTGSEGETVEEKVPDVAAAGDGQQPDAERTVPAEEEPRELTLDEYKALRNAQRMAPQYNLRKAGEGEDLSQ--WKNLVMLEKKKEGGEDDESDEEYDMADYPQRVGRQKRVLGIEFTFS 329          

BLAST of vasa intronic gene vs. nr
Match: gi|1037035762|ref|XP_017079948.1| (PREDICTED: translation initiation factor IF-2 [Drosophila eugracilis])

HSP 1 Score: 136.732 bits (343), Expect = 1.781e-32
Identity = 85/170 (50.00%), Postives = 114/170 (67.06%), Query Frame = 0

Query:  247 GGRGGKRDFDRKSGDDRTSGVKHVDKREGGGSHNWGTFEDEIKAVDDKNNVSTEENPAEAGVERSGDDNAED-QIPREE-EPKTMTLDEWRASQDAKKNAPRFNLRKAGEGAAATEANWKKTYAYKKEKESAMDDEDEEDQELYPQRVNRQRRVLDIEFNFTTEQGSRGG 414
            G + GKR+FDR+SG D+T GVK +DKREGGG+HNWG+ + +I+      ++ T E+  +A  E S ++ + D  IP EE E K MTLDEW+A +D ++  P +NLRKAGEGAA   A WKK     K+KES  +DE E D  LYPQRV R +R++DI+FNF    G +GG
Sbjct:  138 GEKFGKREFDRQSGSDKT-GVKSIDKREGGGAHNWGSPKQDIE------DLKTGESSPQAEKEDSANEQSADPAIPAEEDESKQMTLDEWKALRD-QRAKPNYNLRKAGEGAADN-AEWKKMVVLSKKKESNSEDELEYDPSLYPQRVGRLQRIVDIQFNFN--DGRKGG 296