BLAST of tubulin-specific chaperone d vs. L. salmonis genes
Match: EMLSAG00000001911 (supercontig:LSalAtl2s:LSalAtl2s132:578823:589287:-1 gene:EMLSAG00000001911 transcript:EMLSAT00000001911 description:"augustus_masked-LSalAtl2s132-processed-gene-5.2")

HSP 1 Score: 280.796 bits (717), Expect = 2.913e-79
Identity = 159/459 (34.64%), Postives = 259/459 (56.43%), Query Frame = 0

Query:  732 IQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHELGNSELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGYGPDGLASSTVV-QLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELM---PSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFF---PPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDMSLAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKS-IEILRPMRNNLCDLM 1182
            I++ AI  +  F  EY  D  T  +L+ +R+   ++Y+  L  +   R GF+LALG  P  +L  +   I+E L +C  I   TE WAE+RR+AI  +  I  T+ +         ++ Q+++C + G  DYT++RRGDIGAWVREAA+  L +IT        + +   C + +M   P +A+QAVEKIDRTR LA + F   + +      +P    +   F   P +     F W+ ES+TFP+F +L+  E+Y  ++++GL+VS+GG++  LVK+AS +LF E+ +        F   ++ +L  N  N+R+  P+  F+  L+ +G+ ED L+N     S+D+  ++  EI  S DP+K++   D IC+L Q  DS        +L  +L H++PRIRK+T+ KL+E  LT +D  I+ E + + + +IL+D +WD + I+ LRP++  L   +
Sbjct:  473 IRDFAISTIQPFFQEYFIDSFTKKVLIDRRNFYINKYIQNLDQNFNMREGFTLALGNLPFKILCSKFAPIVEALCKCTRIKNGTEMWAESRRNAIQGICQIFVTMEHQDQNEEFKVLIPQVFECFIEGLNDYTIERRGDIGAWVREAAISGLFTIT-----SYCQKIDFNCSQYIMHFSPLIARQAVEKIDRTRSLAAKTFFKFVRNSFASKEMPDINGILLIFESLPED-----FSWSSESETFPLFVQLIKYEKYRSQLILGLIVSIGGVSFGLVKHASSSLFSELAEADNGFLDSFLINVVDILNTNSGNERIVNPYFGFIKHLVEAGNFEDALKNR----SIDIFSVMNKEITNSKDPKKILSFIDAICSLFQIEDSECQTLIYKKLVGYLFHRYPRIRKMTSCKLYET-LTITDVEIISENDLNKIMSILNDNNWDSTEIKELRPLKTILLSFL 916          

HSP 2 Score: 188.734 bits (478), Expect = 4.231e-49
Identity = 97/206 (47.09%), Postives = 132/206 (64.08%), Query Frame = 0

Query:  436 ALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLS--HVNRDLMKSKVLPKLLHLAQGS-DLFGKHGAIVA 638
            A+  L +    L  +L+ V+ VV  AL+YDE+KG +SVGSH+RDAACYVCW F+R+Y    L P+  + A ALL+V +FDRE+ CRRAA A FQE VGRQ  F  G++IL   DYYAVG+R +TYL LSV ++++ +Y   L  H++  K  HWD+ IR+L++ AL NL+    NRD    + L  L  +A  S D    HG I+ 
Sbjct:  251 AIKHLMKSENTLVRKLNDVMSVVTVALLYDERKGCYSVGSHVRDAACYVCWXFSRSYTKQTLGPYTCDFANALLSVMLFDREITCRRAAXATFQEIVGRQDLFAHGLEILPITDYYAVGNRTHTYLSLSVTISEYPEYTSPLFKHILLIKTMHWDVNIRELSSQALKNLTVFPTNRDYAIKQGLNDLYGIAMKSEDARVIHGVILG 456          

HSP 3 Score: 88.5817 bits (218), Expect = 6.906e-18
Identity = 88/288 (30.56%), Postives = 128/288 (44.44%), Query Frame = 0

Query:   23 ASTEPEDPYTNAVGLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAEHGLERWETVYVLLLWLSMLVLIPFDMGRFDSGAEVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVAR----MGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLS 306
            A  E + P    +GLG  L  F + + + + I  V+           E L QKF   L  YQEQPHLID +   +  +L  ++         G+   +  AA+LA     VR  K   +  PH+   +   L  LE     + WE  Y+LLL +SMLVL+PF M         VSL  RI      YL  T   Q+AA +L +KF TR +     L   + +C    S+    D +     +G LK L ++YK   + ELL +  ++   I    L    N L+R+L+
Sbjct:    4 AMMEEDQP----LGLGSCLNGFVDEEEIGSMIRLVDELEES------EALLQKFKTLLSRYQEQPHLIDQYLEGYFEKLNSVVL-------GGTSQQKRFAASLA-----VRGPKKIHKFMPHDSLLLVKALKNLELLKDTDAWELHYILLLMMSMLVLVPFQMEVLLYTNTEVSLSERIYTSILPYLDTTSATQDAAVLLIAKFATRSDTIQNYLPTVIQYCTQKTSNIKGSDFSSLTSGLGGLKTLCSIYKYSGKPELLPYAQRIFDAI--KHLMKSENTLVRKLN 267          

BLAST of tubulin-specific chaperone d vs. SwissProt
Match: gi|81875711|sp|Q8BYA0.1|TBCD_MOUSE (RecName: Full=Tubulin-specific chaperone D; AltName: Full=Beta-tubulin cofactor D; AltName: Full=Tubulin-folding cofactor D)

HSP 1 Score: 921.383 bits (2380), Expect = 0.000e+0
Identity = 526/1191 (44.16%), Postives = 716/1191 (60.12%), Query Frame = 0

Query:   40 ALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAEH--GLERWETVYVLLLWLSMLVLIPFDMGRFD------SGAEVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVARM----GALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAGE----DADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENL-------SNSDPLIQEKAIKAVPHFLGE-YLKDPKTSTLLVGKRDKIFDEYLHELGN-SELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGYGPDG-----LASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQ-TVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDMSLAQK--QHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEILRPMRNNLCDLMGVPAPAIIKKPAPAT 1197
            ALE F E+    A +  + A   +  R   E   ++F + +D YQEQPHL+DP     +  LL L+++    P+       H A      I KVR  KV +R FPHEV++V+PVL +   ++    E WET Y+LLLWLS+  LIPFD  R D      +G   V    RI  +   YLV + +A++AAA+L SKF TRP++    +  FLDW L   +  +   +  +    G L+ALA ++K G+R + L +   +L  +    L +  +  LR+L +K++QR GL FL  +VA WRYQRG RSL  NL           PG  +  L  +    D D DYD+P+ +E VIE+LL GL+DKDTV+RWSAAKGIGR+  RLPRELADDVV S+L  FS +E+D AWHGGCLALAELGRRGLLLP RL  VV V+LKAL YDEK+G+ SVG+++RDAACYVCWAFARAY+P  L P V  I+ AL+   +FDR VNCRRAASAAFQENVGRQGTFP GIDILT ADY+AVG+ +N +L +SVF+A F +Y   +IDHL+  K+NHWD  IR+L+A ALHNL+    + +   V P LL + Q  DL  +HGAI+A   V  AL K   +    +  YL    V ++K I   L ++   R  GG+L+RQA+   I   SL++ P   D TV  W  ++ + L       S+S   I+E A+ A+     E Y+K+P  +   + K  ++  +YL EL +  E+ R GFS ALGA P F+L+G    +L  L +   I+P+  ++AEARRD + A++ I QT+G    G     +    + ++Y  LLG   DYT D RGD+GAWVREAAM SL  + L L    P L+       +M  +AQQA EKIDR R  A R+F TLL  +   +P +P  QEL+S FP + D+ T  W   SQ FP+ T+LL L  Y   VL+GL VSVGGLTE  V++++ +LF+ +  +    +  Q F   +L+V   N  NDRV++  +K LDQLL +G  +     EN  F + LL L K EI KS D QKL  S  V+C ++Q +  +  KK L+QL + L H FP IRK TA++++E +LT+SD  +V  E  D V ++LSDT WD  + ++R  RN LCDL+GVP P ++ KP P +
Sbjct:   26 ALEAFGESAETRALLRSLPAVHRE--RASREVAEERFRVIMDKYQEQPHLLDPHLEWMMNSLLDLVQDETSLPD-----LVHLAFKFLYIITKVRGYKVFLRLFPHEVANVQPVLDMFTGQNPKDHETWETRYMLLLWLSVTCLIPFDFSRLDGNLSTQTGETRVPTMDRILQIAESYLVVSDKARDAAAVLVSKFITRPDVKQRKMASFLDWSLCTLAHSSFQTIEGVITMDGMLQALAQIFKHGKREDCLPYANTVLQCLDGCRLPESSHTSLRKLGVKLVQRLGLTFLKPKVATWRYQRGCRSLAANL-------KLCAPGKSDQKLLSDSLTSDGDEDYDVPEGVETVIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLPRELADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLSEVVTVILKALTYDEKRGACSVGANVRDAACYVCWAFARAYEPQELTPFVTAISSALVIAAVFDRNVNCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNISNCFLIISVFIAGFQEYTKPMIDHLVSMKINHWDGAIRELSAKALHNLTPQVPEYIAMHVFPALLLMTQSPDLHTRHGAILACAEVTYALYKLATQSNRLVTDYLDEKAVQSLKQIHQQLCDRHLYRGLGGELMRQAVCILIEKLSLSRMPFKGDATVEGWQWLINDTLRSLHLVSSHSRQQIKEVAVSALTALCSEYYVKEPGEAGSSIAK--ELIPQYLAELQSPEEMARCGFSSALGALPGFLLRGHLQQVLSGLRRVTCISPNDVSFAEARRDGLKAISRICQTVGVNTRGPPDEVICKENISEVYAALLGCMSDYTTDSRGDVGAWVREAAMTSLMDLMLLLARTEPVLIEAHICERVMCCVAQQASEKIDRFRAHAARVFLTLLHFDSPPIPHVPHRQELESLFPRS-DVATVNWNAPSQAFPLITQLLGLPTYRYHVLLGLAVSVGGLTESTVRHSTQSLFEYMKGIQKDAQVLQSFSETLLKVFEDNLLNDRVSVSLLKMLDQLLANGCFDIFTAEENHPFCVKLLTLCKEEIKKSKDIQKLRSSIAVLCGMVQFNGDVR-KKILLQLFLLLGHPFPVIRKSTASQVYEMVLTYSD--LVDAEVLDEVMSVLSDTAWDAELPVVREQRNRLCDLLGVPRPQLVPKPIPGS 1196          

BLAST of tubulin-specific chaperone d vs. SwissProt
Match: gi|75039487|sp|Q28205.1|TBCD_BOVIN (RecName: Full=Tubulin-specific chaperone D; AltName: Full=Beta-tubulin cofactor D; AltName: Full=Tubulin-folding cofactor D)

HSP 1 Score: 902.894 bits (2332), Expect = 0.000e+0
Identity = 525/1212 (43.32%), Postives = 720/1212 (59.41%), Query Frame = 0

Query:   19 ASQPASTEPEDPYTN---------AVGLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAEH--GLERWETVYVLLLWLSMLVLIPFDMGRFDS-----GAEVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPA----QPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSN-------SDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHELGN-SELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGY---GP--DGLASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQ-TVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDMS--LAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEILRPMRNNLCDLMGVPAPAIIKKPA 1194
            + +PA+   EDP  +         A+  G ALE+F E+      +  + A    A+R   E   ++F + +D YQEQPHL+DP   +W+  LL+   +N   P        H A      I KVR  K  +R FPHEV+DV+PVL +   ++    E WET Y+LLLWLS+  LIPFD  R D      G E  S   RI  V   YLV + +A++AAA+L SKF TRP++  + +  FLDW L   +  +    +  +A  G L+ALA ++K G+R + L +   +L  + +  L D    LLR+L +K++QR GL FL  +VA WRYQRG RSL  +L   +       P          D+D   D+P+++E VIE+LL GL+DKDT++RWSAAKGIGR+  RLP+ELADDV  S+L  FS +E+D+AWHGGCLALAELGRRGLLLP RL  VV V+L+AL Y+EK+G+ SVGS++RDAACYVCWAFARAY+P  L+P V  I+ AL+  T+FDR+VNCRRAASAAFQENVGRQGTFP GIDILT ADY+AVG+R+N +L +S+F+A F +Y   +I+HL+  KV HWD TIR+L+A AL NL+    +    +V P+LL + Q  DL  +HGA++A   V  +L     ++G  +  +L    +  +K I   L ++   R  GG+L+RQA+   I N +L+K P   D  +  W  ++ + L N       S   I+E A+ A+     EY    +        ++++   YL EL +  E+ R G +LALGA P F LKGR   +L  L     I+P   ++AEARRDA+ A++ I QT+G    GP  + +    V Q+Y  LL   +DYT D RGD+GAWVREAAM SL  +TL L    PEL+     ++LM  LAQQA EKIDR R  A R+F  LL  +   +P +P   EL+  F P   + +  W   SQ FP   RLL L  Y   VL+GL VSVGGLTE  V+Y++  LF+ + ++    A  + F   +L+V   N  NDRV++P +K LDQ+L +G  +     EN  F + LL L K EI KS D QKL  S  V C L+Q    +  +K L+QL + LCH FP IRK TA++++E +LT+    +VP    D V A+LS T WD  + ++R  RN LCDL+GVP P ++ KPA
Sbjct:    4 SEEPAAGAAEDPVEDPVEDAGEDAALACGAALESFGESAETRELLGHLPAVL--ADRSAREGALERFRVIMDKYQEQPHLLDPHL-EWMLNLLLEFVQNKTSPA----DLVHLAFKFLYIISKVRGYKTFLRLFPHEVADVQPVLDMFTNQNPKDHETWETRYMLLLWLSVTCLIPFDFSRLDGNLSQPGQERASTMDRILQVAESYLVVSDKARDAAAVLVSKFVTRPDVKQKKMASFLDWSLCTLARSSFQTIEGVIAMDGTLQALAQIFKHGKREDCLPYAATVLQCLDSCRLPDSNQTLLRKLGVKLVQRLGLTFLKPQVAKWRYQRGCRSLAESLQHSI-----QNPREPVTQAETPDSDGQDDVPEEVESVIEQLLVGLKDKDTIVRWSAAKGIGRMAGRLPKELADDVTGSVLDCFSFQETDSAWHGGCLALAELGRRGLLLPSRLSDVVPVILRALTYEEKRGACSVGSNVRDAACYVCWAFARAYEPQELKPFVAAISSALVIATVFDRDVNCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNRSNCFLVISMFIAGFPEYTQPMIEHLVTMKVGHWDGTIRELSAKALRNLAQRAPEHTAREVFPRLLSMTQSPDLHTRHGAVLACAEVARSLHTLATQQGRPVSDFLDEKAMHGLKQIHQQLYDRQLYRGLGGELMRQAVCILIENVALSKMPFRGDAVIDGWQWLINDTLKNLHLISSHSRQHIKEAAVSALAALCSEY-HAQEPGEAEAAAQEELVKLYLAELQSPEEMTRCGCALALGALPAFFLKGRLRQVLAGLRAVTHISPKDVSFAEARRDALKAISRICQTVGVRAEGPPDEAVCRENVSQIYCTLLDCLKDYTTDSRGDVGAWVREAAMTSLMDLTLLLGRNQPELIEAPLCQQLMCCLAQQASEKIDRFRAHAARVFLALLHADSPAIPHVPARPELERLF-PRAAVASVNWGAPSQAFPRMARLLGLPAYRYHVLLGLAVSVGGLTESTVRYSTQGLFEYMKEIQNDPAALEDFGGTLLQVFEDNLLNDRVSVPLLKTLDQMLANGCFDIFTAQENHPFCVKLLALCKEEIKKSKDVQKLRSSIAVFCGLVQFPGDVR-RKVLLQLFLLLCHPFPVIRKNTASQVYEMVLTY---DVVPTAVLDEVMAVLSSTAWDAELPVVRAQRNRLCDLLGVPRPQLVPKPA 1197          

BLAST of tubulin-specific chaperone d vs. SwissProt
Match: gi|296452924|sp|Q9BTW9.2|TBCD_HUMAN (RecName: Full=Tubulin-specific chaperone D; AltName: Full=Beta-tubulin cofactor D; Short=tfcD; AltName: Full=SSD-1; AltName: Full=Tubulin-folding cofactor D)

HSP 1 Score: 892.878 bits (2306), Expect = 0.000e+0
Identity = 523/1209 (43.26%), Postives = 730/1209 (60.38%), Query Frame = 0

Query:   19 ASQPASTEPEDPYTN-AVGLGCALETFQEAQAVEAWIEGV-EAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAEH--GLERWETVYVLLLWLSMLVLIPFDMGRFDS------GAEVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPA----QPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENL-------SNSDPLIQEKAIKAVPHFLGE-YLKDPKTSTLLVGKRDKIFDEYLHELGN-SELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGY---GPD-GLASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQ-TVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQ-----EVDDMSLAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEILRPMRNNLCDLMGVPAPAIIKKPA 1194
            + +PA+  PE+   +  +  G ALE F E+    A +  + E     A R   E   ++F + +D YQEQPHL+DP   +W+  LL+ I ++   P +      H A      I KVR  K  +R FPHEV+DVEPVL L+  ++    E WET Y+LLLWLS+  LIPFD  R D       G   +S+  RI  +   YL+ + +A++AAA+L S+F TRP++    +  FLDW L   +  +    Q  +   G L+ALA ++K G+R + L +   +L  +    L +    LLR+L +K++QR GL FL  +VAAWRYQRG RSL  NL  +L     SE   Q   +  ED D D D+P+ +E VIE+LL GL+DKDTV+RWSAAKGIGR+  RLPR LADDVV S+L  FS +E+D AWHGGCLALAELGRRGLLLP RL  VV V+LKAL YDEK+G+ SVG+++RDAACYVCWAFARAY+P  L+P V  I+ AL+   +FDR++NCRRAASAAFQENVGRQGTFP GIDILT ADY+AVG+R+N +L +SVF+A F +Y   +IDHL+  K++HWD  IR+L A ALHNL+    +   ++V P+LL +    DL  +HG+I+A   V  AL K   +E   +  +L    V  +K I   L ++   R  GG L+RQA+   I   SL+K P   D  +  W  ++ + L       S+S   +++ A+ A+     E Y+K+P  +   +  ++++  +YL EL N  E+ R GFSLALGA P F+LKGR   +L  L      +P+  ++AE+RRD + A+  I QT+G     PD  +    V Q+Y  LLG   DYT D RGD+G WVR+AAM SL  +TL L    PEL+       +M  +AQQA EKIDR R  A  +F TLL  +   +P +P   EL+  FP + D+ +  W+  SQ FP  T+LL L  Y   VL+GLVVS+GGLTE  +++++ +LF+     + D  +L     F   +L++   N  N+RV++P +K LD +LT G  +     E+  F++ LL L K EI  S D QKL+    V C ++Q    +  ++AL+QL + LCH+FP IRK TA++++E LLT+SD  +V  +  D V  +LSDT WD  + ++R  RN LCDL+GVP P ++ +P 
Sbjct:    4 SDEPAAGGPEEEAEDETLAFGAALEAFGESAETRALLGRLREVHGGGAER---EVALERFRVIMDKYQEQPHLLDPHL-EWMMNLLLDIVQDQTSPAS----LVHLAFKFLYIITKVRGYKTFLRLFPHEVADVEPVLDLVTIQNPKDHEAWETRYMLLLWLSVTCLIPFDFSRLDGNLLTQPGQARMSIMDRILQIAESYLIVSDKARDAAAVLVSRFITRPDVKQSKMAEFLDWSLCNLARSSFQTMQGVITMDGTLQALAQIFKHGKREDCLPYAATVLRCLDGCRLPESNQTLLRKLGVKLVQRLGLTFLKPKVAAWRYQRGCRSLAANL--QLLTQGQSE---QKPLILTEDDDEDDDVPEGVERVIEQLLVGLKDKDTVVRWSAAKGIGRMAGRLPRALADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLVDVVAVILKALTYDEKRGACSVGTNVRDAACYVCWAFARAYEPQELKPFVTAISSALVIAAVFDRDINCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNRSNCFLVISVFIAGFPEYTQPMIDHLVTMKISHWDGVIRELAARALHNLAQQAPEFSATQVFPRLLSMTLSPDLHMRHGSILACAEVAYALYKLAAQENRPVTDHLDEQAVQGLKQIHQQLYDRQLYRGLGGQLMRQAVCVLIEKLSLSKMPFRGDTVIDGWQWLINDTLRHLHLISSHSRQQMKDAAVSALAALCSEYYMKEPGEADPAI--QEELITQYLAELRNPEEMTRCGFSLALGALPGFLLKGRLQQVLTGLRAVTHTSPEDVSFAESRRDGLKAIARICQTVGVKAGAPDEAVCGENVSQIYCALLGCMDDYTTDSRGDVGTWVRKAAMTSLMDLTLLLARSQPELIEAHTCERIMCCVAQQASEKIDRFRAHAASVFLTLLHFDSPPIPHVPHRGELEKLFPRS-DVASVNWSAPSQAFPRITQLLGLPTYRYHVLLGLVVSLGGLTESTIRHSTQSLFEYMKGIQSDPQALGS---FSGTLLQIFEDNLLNERVSVPLLKTLDHVLTHGCFDIFTTEEDHPFAVKLLALCKKEIKNSKDIQKLLSGIAVFCEMVQFPGDVR-RQALLQLCLLLCHRFPLIRKTTASQVYETLLTYSD--VVGADVLDEVVTVLSDTAWDAELAVVREQRNRLCDLLGVPRPQLVPQPG 1190          

BLAST of tubulin-specific chaperone d vs. SwissProt
Match: gi|82081029|sp|Q5ZI87.1|TBCD_CHICK (RecName: Full=Tubulin-specific chaperone D; AltName: Full=Beta-tubulin cofactor D; AltName: Full=Tubulin-folding cofactor D)

HSP 1 Score: 794.267 bits (2050), Expect = 0.000e+0
Identity = 449/1007 (44.59%), Postives = 617/1007 (61.27%), Query Frame = 0

Query:   23 ASTEPEDPYTNAVGLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALL--EAEHGLERWETVYVLLLWLSMLVLIPFDMGRFDS------GAEVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPA----QPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAGEDA--DADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPL--------IQEKAIKAVPHFLGEYL--KDPKTSTLLVGKRDKIFDEYLHELGNSE-LHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGYGPDG-----LASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQ-TVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTE 998
             S E      + +  G  LE+F E+Q V A +  +       + +  E + +KF + +D YQEQPHL+D    +W+  +L+ I  + G P        H A      I KVR  K+ +R FPHEV+D++PVL ++  +     E WET Y+LLLWLSM+ LIPFD+ RFD       G   +    RI  + + YLV + +A++AAA+L SKF  RP++  + +  FLDW LS+ S  +    +  V   G L+ALA ++K G+R + L +   +L  +   +L +    +LR+L +K++QR GL F+  +VA WRYQRG RSL  NL A+      S    Q + +A  +A  D +YDIP +IE V+E+LL GL+DKDT++RWSAAKGIGRIT RLP+ELADDVV SLL  FS +E+D AWHGGCLALAELGRRGLLLP R+  VV V+LKAL YDEK+GS SVGS++RDAACY+ WAFARAYDP  L P +++I+ AL+   +FDR+VNCRRAASAAFQENVGRQGTFP GIDILT ADY+AVG+R N YL +SV++A F +Y   +IDHL+  K+NHWD  IR+L+  ALHNL+    + M + VLP+LL L+ G+DL  +HGAI+A   +  AL K  E+    +  Y     +  +K I   L  +   R  GG+L+R A+ + I   SL+K P   D  +  W  ++ ++L  S PL        ++E A+ A+     EY   ++ +    L G   ++  +Y+ EL ++E + R GFSLALGA P F+LKGR   +LE L +   ITP   ++AE+RRDA++A+  I QT+G   +G     +    V Q+Y  LL    DYT D RGD+G WVREAAM SL  +TL L+    EL+     +++M  LAQQ+ EKID+ R  A  +F TLL  +   VP IP  +EL+  FP + +  T  W   S+ FP  T+LL L  Y   VL+GL VSVGGLTE
Sbjct:   10 GSEESGSREADVISRGNILESFTESQEVRALLGNLRTV--YGDPVAQEVIVEKFIVIMDKYQEQPHLLDRHL-EWMMNMLLDIIRDSGSPPV----LFHLAFKFLYIITKVRGYKLFLRLFPHEVTDLQPVLDMIVDQNPKDCETWETRYMLLLWLSMICLIPFDLARFDGNILSEEGHTRMPTMDRILEIAKCYLVVSDKARDAAAVLVSKFIVRPDVRQKRMADFLDWTLSMLSKSSFQSMEGTVVMNGMLQALAQLFKHGKREDCLPYAATVLECLDNCKLSESNQMVLRKLGMKLVQRLGLTFVKPKVAKWRYQRGCRSLAANLQAQ-----SSVMQSQKITVAANEAEDDEEYDIPGEIENVVEQLLVGLKDKDTIVRWSAAKGIGRITGRLPKELADDVVGSLLDCFSFQETDNAWHGGCLALAELGRRGLLLPSRISDVVPVILKALTYDEKRGSCSVGSNLRDAACYLSWAFARAYDPSELIPFINQISSALVIAAVFDRDVNCRRAASAAFQENVGRQGTFPHGIDILTAADYFAVGNRVNCYLTISVYIAGFPEYTQPMIDHLVNMKINHWDSVIRELSTKALHNLTPRAPEYMANVVLPRLLPLSVGTDLHTRHGAILACAEITHALCKLAEENNRSITYYFNGKSLEGLKQIHQELCSRQLYRGLGGELMRPAVCTLIEKLSLSKMPFKGDPIIEGWQWLINDSL-RSLPLASCAARQHVKESAVSALSALCNEYYINENGEADPALQG---ELVTQYISELQSTEQMIRCGFSLALGALPRFLLKGRLQQVLEGLRKVTLITPRDVSFAESRRDALIAIAEICQTVGVKGEGSQEEYICKDNVAQIYATLLNCVTDYTTDSRGDVGGWVREAAMTSLMKVTLLLVQNEAELINANICKQIMCWLAQQSAEKIDKFRAHAGSVFLTLLHFDSPPVPHIPHREELERIFPRS-EAETLNWNAASEAFPRITQLLALPAYQYYVLLGLSVSVGGLTE 999          

BLAST of tubulin-specific chaperone d vs. SwissProt
Match: gi|75153882|sp|Q8L5R3.1|TBCD_ARATH (RecName: Full=Tubulin-folding cofactor D; Short=AtTFCD; AltName: Full=Protein CHAMPIGNON; AltName: Full=Protein EMBRYO DEFECTIVE 133; AltName: Full=Protein TITAN 1)

HSP 1 Score: 607.446 bits (1565), Expect = 0.000e+0
Identity = 415/1187 (34.96%), Postives = 620/1187 (52.23%), Query Frame = 0

Query:   74 QKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGS---LGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAEHGLE-----------RWETVYVLLLWLSMLVLIPFDMGRFDSGA---------EVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDW---CLSVASDPAQPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDH--PNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLA----ELAPAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYL--KDPKTSTLLVGKRDKIFDEYLHELGNSELH-RRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETW-AEARRDAILALTSIAQTLGY------GPDGLASSTVV--QLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSL--CSITL----------DLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQ-TVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQ-----EVDDMSLAQKQHFCRA--MLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFS-----MDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEILRPMRNNLCDLMGVPAPAIIK 1191
             K    +D YQEQ  L++P+    +  L+ +IR      EA     L      + +   ++ V   K  ++ FPH+VSD+E  + LLE  H                E   V LLWLS+LVL+PFD+   D+           ++  L  +I    + YL +    +  + +L SK  TRP++  +    F +W    LS   D        +G ++AL+A++K   R  LL   P +L  +  L   +    ++LLR+  +K+ QR GL+ LP R  +WRY     SL  N+       LAP        Q   L  ++ D D D+P+ +EE+IE LLSGLRD DTV+RWSAAKGIGR+T+RL   L+D+V+ S+L LFS  E D +WHGGCLALAEL RRGLLLP     VV V++KAL YD ++G  SVGSH+RDAA YVCWAF RAY    ++  +D++A  LL V  FDREVNCRRAA+AAFQENVGRQG +P GIDI++ ADY+++ SR N+YL ++V +AQ+  Y    +D L+  K+ HWD ++R+L A+AL  L         + VL KL+     +DL  +HGA +A G V LAL +         G  L  D    +  I P++ +    R  GG+++R A++ +I   SL+   +  + T  +    L ENL + +  IQ  A+ AV   +  YL   D K+  L++        ++L  L +  +  RRG +LALG  P  +L  +  DI+ KL     I  + E   AEAR +A+  LTS+ +TL        G D L+   ++  ++   LL    DY++D RGD+G+WVREAA+  L  C+  L          D  D +  L        L+  + +Q VEK+D+ R  A ++   +L H+  +VP +P  ++L+   P   +     W V + +FP F +LL L  Y++ V+ GLV+S+GGL + L K + +AL +     E  D    Q +       +L +L+  +K DRV +P ++ ++ L +S   +  L  E+  FS     MD L +   E+  S D  KL      I   + +       KA  QL  FL H++P IRK  A +++ ALL   +  +V EE  + V  I+S++ W+  +E  +  R  LC+L G+    + K
Sbjct:   67 HKIRSIMDKYQEQGQLVEPYLESIVSPLMFIIRSKTVDLEAKPDEILEIIKPISIIIYALVTVCGYKAVIKFFPHQVSDLELAVLLLEKCHSTNSVSALRQESTGEMEAKCVTLLWLSILVLVPFDISSVDTSIADDKTFGVDDLAPLVLKILGFCKDYLCSAGPMRRISGLLLSKLLTRPDMG-KAFSSFFEWTYEVLSCKEDSVTNHFRLLGVMEALSAIFKTASRKVLLDVLPIVLNDVTVLSKSNAAAKSSLLRKYLIKLTQRIGLVCLPHRSPSWRYVAQTASLSENMSTSSSQRLAPDHTVTAILQPESLDDQE-DEDMDVPEILEEIIEMLLSGLRDTDTVVRWSAAKGIGRVTSRLTSVLSDEVLSSVLELFSPGEGDGSWHGGCLALAELARRGLLLPRSFPLVVPVIVKALHYDVRRGPHSVGSHVRDAAAYVCWAFGRAYSHKDMKNVLDQLAPDLLIVGSFDREVNCRRAAAAAFQENVGRQGNYPHGIDIVSIADYFSLSSRVNSYLQVAVSIAQYEGYLYPFVDELLYNKICHWDKSLRELAAEALAPLVKYEPKHFANYVLEKLIPCTLSTDLCMRHGATLAAGEVVLALHQC--------GYVLSADSQKRMAGIVPSIEKARLYRGKGGEIMRLAVSRFIECISLSHVTL-AERTERILLDTLTENLRHPNSQIQNAAVSAVKQLVQSYLVGNDKKSVDLIL--------KHLKHLTDPNVAVRRGSALALGVLPYELLTAKWKDIVLKLCSACKIEVNPEDRDAEARVNAVKGLTSVCETLTQKRASDPGNDDLSLFLLIKTEVMDTLLKALDDYSVDNRGDVGSWVREAAVHGLEKCTYILCKKMETYSEGDYNDDTSSLFDSNLATRLIGGMLKQGVEKMDKLRETAAKVLQRILYHKSVSVPHVPYREKLEEILP---NKANLQWAVPAFSFPRFVQLLKLRCYSKEVMSGLVISIGGLQDSLRKASLVALLEYMREGEAKDPKEQQSRESALGDDILWILQEYKKCDRVMVPCLQTIEILFSS---KIFLNQESYTFSFYAGVMDSLAI---ELRASKDFTKLKAGL-AILGYIASVSHFISTKAFSQLLSFLGHRYPMIRKAAAEQVYLALL--QNGILVTEEKMEKVIEIISESCWEADMETTKTQRLELCELAGLDHEVVFK 1222          

BLAST of tubulin-specific chaperone d vs. SwissProt
Match: gi|74963837|sp|Q19493.1|TBCD_CAEEL (RecName: Full=Tubulin-specific chaperone D; AltName: Full=Tubulin folding cofactor D homolog)

HSP 1 Score: 363.999 bits (933), Expect = 1.249e-104
Identity = 337/1156 (29.15%), Postives = 538/1156 (46.54%), Query Frame = 0

Query:   62 NQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLL---ILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLE--AEHGLERWETVYVLLLWLSMLVLIPFDMGRFDSGAEVVSLRRRIDAVTRQYLVAT-VRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIAL-ELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAGE------DADADYDIPDDIE--EVIE----ELLSGLRDKDTVIRWSAAKGIGRITNRLPR-ELADDVVESLLG-LFSLRESDAAWH--GGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVN----RDLMKSKVLPKLLHLAQGSDL--FGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYLKD-----PKTSTLLVGKRDKIFDEYLHELGNSELHRRGFSLALGACPEFVLKGREGDILEKLFQCI--PITPDTETWAEARRDAILALTSI---AQTLGYGPDGLASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNL-EEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDMSLAQKQ--HFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTS-GHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQT-HDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDW-DKSIEILR 1172
            N+ +    E  Y +++  L LYQEQP L+D + P+ +  L+    LI  +  +P A +  +R +   L+   I VR  K  VR  PH+V  ++P+L  LE      L       VLL+WL ++V  PFD+ RFD   +  ++  RI  V   Y+      +Q +AA++ +   +R +   ++L  FL   L       +     +  L  L A+ K   R  L      +  ++  L  + +    L+ +  +KV+QR GLI L  R  +W Y RG R L+  +L +       EP   N   + +      D +  ++  D++E  E++E     +L  L   DT +RWSAAKG+GRIT RLP  +LA  VV S++   F      ++WH  G CLALAEL  RG+LLP  L+ +V  +  +LV+++  G    G+ +RDAACY  WA +R Y+P  + P++  +A ALL   +FDR+VN RRAASAA QE VGRQ     GI ++   DY+AV +R   Y  L V VA++  Y+  ++ HLI KKV HWD  IR+  A +L  +S +      D    ++L   L  +  + +  F +HG ++A G     L K +   G D          + I  I   L     +    G L+R+ +  +I   S +K  +  +     W  +L + +++   +I+  A  AV  F+  YL +      K  T ++    K  DE           R G  +    C     +  + ++ E L   I  P + D + WA AR+  + AL  I   + T  +   G       + ++ L     DYT    GDIG +VREA+M ++ +I +D   + P L   +   +    + QQ+ E+I RTR  A     +L+  E T   +P    L + +   +D       +  +T      LL+L  EY E++++G+VVS GGL E   K A   L     ++   + +  HF      + +  +K +R+   FM+ L Q+  + G  E   E   S   M  +  +K    +S    +  LS D +  LL     S   + AL  +   L  + P +RK  A +L+E L          EE+ D V  +L+ T+W D++  +L+
Sbjct:  114 NECDETAAEVNYLRYSRLLHLYQEQPRLLDKWIPEIVANLVDLVTLIGIDVSKPRAMTPLSRESLKYLSDLCI-VRGSKTIVRLLPHQVHLLDPLLQTLEYYETSQLSDHNQRNVLLMWLWIVVKNPFDLRRFDPTGDPDNVITRIMNVALHYMKWDWNSSQASAALVIAHCLSRTDGIPKVL-SFLSRLLDSIKTHHENKKLLLADLILLLAILKHVDRRVLTGHIGTIHEQLSFLYPIDEKKGGLICKCLVKVVQRIGLIALKPRTCSWSYNRGKR-LLEGMLDD-NEEYSDEPSFSNKVNSNQSCNNEIDKENQWNDGDELENSEIVEFALMHVLEALSHSDTAVRWSAAKGVGRITVRLPNFDLATQVVGSIISSHFGEVAEYSSWHSHGACLALAELAHRGVLLPSLLEDIVPALELSLVFEDVMGRHQNGNQVRDAACYAVWALSRTYEPSMMAPYLQRLASALLCGALFDRQVNLRRAASAALQEMVGRQKNVSHGIPLIQSVDYFAVTNRQKCYEHLCVPVAEYSTYSAIILRHLITKKVVHWDEKIREQAAISLEKISEIRLENVSDDYYMEILDDFLKASCETRISPFLRHGYLLASG----HLIKGLTSRGMDFSS-----KQTEIAWIPHILWPFCDMTTQPGALIRRTLCKFIQLVSASKKVLLLEKDKSEWLDVLLQLITDPREIIRSLAKTAVGEFVMTYLMNDEELIQKVKTRVIAAMTKCSDES---------ERIGMGM---ICESLNSEAVDYEMFESLCNTILTPTSSDAK-WALARQQTVFALNRISVNSSTETFNRIG------QKCFETLYKAMTDYTTSANGDIGRFVREASMRAMSTILVDAKTEPPFLDEHVI--KSAKYMVQQSAERISRTRECACACLKSLVKCEITGRCLPHIDLLMNIYSEPMDF------ISDRTVFQLKPLLDLGSEYYEQLILGIVVSAGGLAEGTQKTAKQLLLDHQREICENKPRFDHFLSTCADLFQRARKVNRIGNSFMQILPQIFGNLGIYEQCPETSESIIEM--VDTMKTIAVRSSMMSRQRLSIDSLGELLNCGKKSTVYRSALTMILDTLNSQQPVLRKSAAERLYEHL-------CCAEESDDEVLEVLATTNWQDENDNVLK 1220          

BLAST of tubulin-specific chaperone d vs. SwissProt
Match: gi|206558247|sp|Q55G93.2|TBCD_DICDI (RecName: Full=Tubulin-specific chaperone D; AltName: Full=Tubulin-folding cofactor D)

HSP 1 Score: 357.451 bits (916), Expect = 3.705e-101
Identity = 326/1183 (27.56%), Postives = 553/1183 (46.75%), Query Frame = 0

Query:  158 EAEHGLERWETVYVLLLWLSMLVLIPFDMGRFDSGA-----------------EVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLS-------------VASDPAQPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIA----LELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAP-AAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLG-LFSLRE----SDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGR-QGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFG-DYNVALIDHLIEKKV----------------------------NHWDLTIRDLTADALH-------------------NLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIG------TVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSL--RMTGGDLLRQAITSYISNCS-------LAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHELGNSELHRRGFSLALGACPEFVLKGREGDILEK--------LFQCIPITPDTETWAEARRDAILALTSIA------------QTLGYGPDGLASST---VVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLM-------------------DKSPE-LVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQ--TVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDMSLAQKQH------FCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILE----NENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICAL---LQTHDSLAVK--KALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIE-ILRPMR 1175
            E    +  WE VYVL LW+S+LV+IPF     DS +                 ++ S+  RI  + +  L    + +++ + L SK   RP++  E  + F+ WC +               +      +  +G    LA ++K+G R + L     L  +I+     L L      + +++ LK++QR  +I LP   A+WRYQ+  + L+  L  EL      +     N +   E  + + +IP++I+E++EE++  L+DKDT+IRW++AK IGRI N LP+++ D V+  ++  +F   E      +AWHGGCLALAEL RRGLLLPERLD VV +V++AL +D  KG++S+GSH+RD+ACY+CWA AR Y    L P++  I   L+ V+++DRE+NCR++ASAAFQE VGR QG  P GI+I+T AD++ VG++NN++  L+ F+ +F  DY   +I HL   K+                            N+  L I +  +D +H                   N  ++  D +K  +L  + +        GK G ++ IG      ++CL +  +++K  S++ +       +  +  A AL  K+++    T   + +  IT   S  +              ++   ++    L ENL++ +  +Q++A KA      +Y+   +  +LL+   D        ++  S   RRG SL LG+ P F       D+L K        +FQ  P   D ET    R ++I +L  I                    D   S      ++++ CL     DY++D+RGDIG+WVRE +    C +  D +                   D S E L+ E  + E +  L Q + EK+D+ R +A ++   LL  E   ++  IP  +ELK     + D+  F W    ++ P+  ++L    Y   +L GL  S+GG ++ L+  +  ++ Q        +K+       F +A+L +   N    R+  P  + +  LL S H+ D L     NE S F   L    +   +   D   L+ S ++        + +++  +K       L +    K+P++RKL +++L ++   F + +   +E    + +++ +T WD S++ I+ P++
Sbjct:  234 EYNENIISWEEVYVLALWVSLLVIIPFKFSSLDSASSGTASAAGDGGDGDGDGQLKSISSRILKLGKLALSDVSKIRDSFSELLSKLLNRPDMKFEQ-KQFIKWCTNSIQLISNNNNNNNQNNSSNNNILLIIGIYSTLATMFKKGNRLDFLPIDMNLYEKIMEANKYLSLSGSE-RITKKIFLKLLQRIAIIMLPPVSASWRYQKIIKPLL--LKGELIKQINNNNNNNNNENNNEEGEEEEEEIPEEIDEILEEIMKSLKDKDTIIRWTSAKAIGRIVNLLPKDMGDQVIGLVIDQMFEKNEFIDADPSAWHGGCLALAELARRGLLLPERLDVVVPLVIRALFFDIIKGTYSIGSHVRDSACYLCWALARTYHNSILSPYLLPICRNLVVVSLYDREINCRKSASAAFQEMVGRHQGLVPNGIEIVTSADFFTVGNKNNSFTSLTTFIGKFQIDYYPIVIKHLATIKIYNWDLEIRQLASKSIHLLTNINPNDIVSNYLPLIIPNTQSDLVHVKHGASLAISEILISLFENNNINLLSDNLKMMILMTIKNTKNEKLFKGKGGVLIRIGMCKIIYSICL-VEFSLDKNLSEIKKPTESTSTNGNEDRAAALKLKIAMLKAKTASQINKPIITPPSSKSTTNNNNNNNNNNLNDNEIAFNIILGYLNENLNHPNEEVQKEASKAFELLFSKYISSNEKISLLLELIDSHCKTLKFDINRSA--RRGSSLLLGSLP-FNSANLSYDLLSKVVNELILSIFQDDPKFKDIET----RVNSISSLYKIGIYILNLIFKNQENEQKEEEDFKKSKNYNLFIKIWNCLGLATNDYSIDKRGDIGSWVRELS----CKVLFDFIKFIITNQNSSTTTTTASTTDLSIENLINEKMITEFICKLFQLSGEKLDKIRDVACKIIHELLWIENPSSINNIPHKEELKKIIVKDQDV-HFNWFRTEESLPLICKVLKFNCYLYPLLFGLFSSLGGTSKYLINDSIQSIKQYFSSFDNDEKERFEKIISFSKAILEI--TNNTTQRMIQPTFRSIYNLL-STHIFDFLIINNLNEQSIFETILFNCYQIIESNQDDIYLLLNSIELFSYFFIQFENNNNEYIKDYSLKALLLLLSNLKYPKVRKLASDQLKKSTRLFINNN-GDDETPSLIKSLIFNTKWDDSVDLIIEPLK 1395          

BLAST of tubulin-specific chaperone d vs. SwissProt
Match: gi|408360324|sp|Q10197.3|ALP1_SCHPO (RecName: Full=Tubulin-folding cofactor D; AltName: Full=Altered polarity protein 1)

HSP 1 Score: 186.422 bits (472), Expect = 1.377e-46
Identity = 144/552 (26.09%), Postives = 250/552 (45.29%), Query Frame = 0

Query:   80 LDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAEHGLERWETVYVLLLWLSMLVLIPFDMGRFDSGAEVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIALELKD---HPNALLRQLSLKVIQRCGLIFLPARVA-AWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAGEDADADYDIPDD--------IEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLR--------------ESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLS 605
            L   Q QP L+D    +++  L   + +        ++G  ++   +     K+R  K     FP  V  ++ +  LL  E     W   Y++LLWLS  +  PF +   D   +V   ++ I  +  +YL  +   + A+ ++ S+ F+R +    LL   L  C S  S   +    ++G L +L++  K   R + L+ T  +  + + +  +D     N+ LR+L  K   R G++ LP   +  W+Y   N                               D+ + +PDD        +E +++ LLS + D D+ +RWSAAKG+ +I +RLP  LA+ V+++++ L +                 S   WHG  L  A+L   GL+   +   ++ ++   L Y+ + G+   G  IRDA+CY  W+F   Y   A+      +   LL   +FD E+N RRAA+AA  E +GR  + P G+ +++  +Y +V   +N Y DL + VA F  +   +   L    + HWD+ ++ L+A +L  LS
Sbjct:   50 LQFCQFQPTLLDKLLSKYVPNLASYLLK------VKNIGKCNSITVILYQFCKIRGYKAVRVLFPVGVQYIKELYTLLN-ESSNNTWHFHYIVLLWLSQALNTPFPLNSLDDSLDV---KKTIYTIAIKYLENSGIDKEASCLVLSRLFSRDDGLDLLLGF-LHHCES--SWFKRSIFYKIGCLFSLSSFLKICPRNDCLQ-TVDVAFQFLNVAREDLVGQENSALRKLLCKCYTRLGIVLLPVNSSPNWKYSISN------------------------------PDSFFQLPDDSNEEVHIYLEVIVDFLLSSVSDIDSFVRWSAAKGLAKIISRLPWNLAEQVIDAIIELMTENMFLNPIENTVNISITSPLVWHGAILFFAKLAGAGLIKYSKCLHILPLIEVGLSYEVRYGTRVTGQSIRDASCYFVWSFYHCYSKSAIEGLQTNLILCLLQTVLFDNEINVRRAATAALFEVIGRHASIPDGLSLISHINYVSVTDISNCYGDLCMKVAHFPQFRSCVFQRLF-TNLQHWDVKVQQLSAFSLRQLS 556          

BLAST of tubulin-specific chaperone d vs. nr
Match: gi|1325261861|ref|XP_023331633.1| (tubulin-specific chaperone D-like [Eurytemora affinis])

HSP 1 Score: 1141.33 bits (2951), Expect = 0.000e+0
Identity = 598/1180 (50.68%), Postives = 799/1180 (67.71%), Query Frame = 0

Query:   24 STEPEDPYTNAVGLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEA--EHGLERWETVYVLLLWLSMLVLIPFDMGRFDSGAEVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPA--QPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNL-LAELAPAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHELGNSELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGYGPDGLASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHE----QTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDMSLAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDW-DKSIEILRPMRNNLCDLMGVPAPAIIKKP 1193
            +TE E+     +GLGCAL+ F + + +   +    A  +  N    E + +++   LDLYQEQPHLIDP     +  L+ +IR++   P+       +    L  H+ KVR  K+ VR FPHEV D+E V++LL+A  E   E WET YVLLLWLS++V+IPF+M RFDSG +   L  RI  + + +L +  ++++AA+ LASKF TRP+     L  FLDW L  A+     +PD+   G L AL++V K  +R +LL F P +L RII    K+H N  +R+LSLK+IQR GL+FL +++A WRYQRG+RSL +NL LA + P     PG + +DL  ED    YD+P+ +EEVI+ELL+GL+D++TV+RW+AAKGIGR+T RL +ELAD+VV SLL LFS RE+D+AWHGGCLA+AELGRRGLLLP RL  VV  ++KALVYDEK+GSFSVGSHIRDAACYVCWAFARAY P+ L+P V ++A +LL VT+FDREVNCRRAASAAFQENVGRQGTFP GIDILT ADY+ VG R+N YL +S +VA+FG+Y+  LI HL+E+KV HWD  +R+L A AL+ L+H ++  M S  LP LL +   SDLF  HGA +A+G   LALS+   K+G  + + LG D+   I T+   L+ K  LR  GG+L+RQA + +I N S+A  P+     V  W  +L ENLS+ D  +Q  ++ A+P F  +Y  +     L +  RD I  +Y   L  +E HR+GFSLALGA    ++ GR+ DI+  L +C  IT  TE WAE RRDA+ ALTS+   L      +++  +  +Y C +   +DYT+DRRGD+GAWVREAA+  +   TL L+      +P   V + MP LAQQAVEKIDRTRG A R+F T+L  +     TVPGI   ++L   FP N D+    W VES+TFP+F ++L L+EY+ER+L+GLVVSVGGLTERLVK +S +LF E++ MS  + + FC  +L +  A QKNDRVTLPF+KFLDQ+ TS  L+ +L++ NS F +DLL L K E+ K GDP KLM S +V C LLQ  +    +K LVQ++IFLCH+FPR+RK +A+KL+EALLTF+DR IVPEEN D V  +LS+T+W + SI+ LR +RN +C+L GVPAPAI K+P
Sbjct:    6 TTEDEN-----IGLGCALDKFGDLEEINKMMGA--AWEHLENERDEEQILERWKYVLDLYQEQPHLIDPHLDTILGNLVKMIRKHTTNPQL------YITTRLLFHLTKVRGYKIVVRHFPHEVDDLEFVVSLLQAQDEKNTETWETRYVLLLWLSIIVIIPFNMSRFDSGNQE-PLSERILTLCKVHLTSRDKSRDAASYLASKFLTRPDTRDTSLPAFLDWALVTATKDGAREPDIT--GGLMALSSVVKHSKREDLLEFAPAVLTRIIKSGFKEHTNTFIRKLSLKLIQRLGLVFLKSKIATWRYQRGSRSLTINLNLAPVQPV----PGVKEVDLEEED----YDVPEIVEEVIDELLNGLKDRETVVRWTAAKGIGRVTGRLAQELADEVVGSLLELFSDRETDSAWHGGCLAVAELGRRGLLLPIRLGKVVEFIIKALVYDEKRGSFSVGSHIRDAACYVCWAFARAYAPEVLQPFVQDLASSLLIVTVFDREVNCRRAASAAFQENVGRQGTFPHGIDILTAADYFTVGQRSNAYLVISEYVARFGEYSKPLISHLVERKVGHWDAAVRELAAKALNRLTHCDQLYMLSYGLPSLLDMCVSSDLFSSHGATLAVGQTVLALSQIALKDGLSVNKILGDDLTEKIATLMDRLILKNKLRGLGGELMRQAASDFIENSSMAGLPL-PPSAVTTWKTVLDENLSDMDQRVQSASVSAIPAFTSQYYIN--NGILDLNARDTILTQYFTMLKGNEQHRKGFSLALGALNTKLILGRQEDIINNLIRCSRITEGTEKWAEGRRDALKALTSVGTKLDR---YISNELIHHIYDCFMISLEDYTVDRRGDVGAWVREAALTGIELFTLKLLSWDSSKIPPSIVGQFMPCLAQQAVEKIDRTRGHAGRIFHTILHAKSSEGNTVPGIRVREQLLEIFPENCDV---KWAVESETFPMFVKMLRLKEYSERMLLGLVVSVGGLTERLVKNSSQSLFNELNSMSSEEIKLFCSNILSLFNAFQKNDRVTLPFLKFLDQVFTSSCLDSVLQDPNSGFPLDLLLLCKNELVKCGDPNKLMASSEVFCQLLQAENPQCPRKCLVQIAIFLCHRFPRVRKYSADKLYEALLTFADREIVPEENLDEVMNLLSETNWIETSIDELRGIRNKICELSGVPAPAIKKQP 1152          

BLAST of tubulin-specific chaperone d vs. nr
Match: gi|1242753973|ref|XP_022315849.1| (tubulin-specific chaperone D-like [Crassostrea virginica])

HSP 1 Score: 998.423 bits (2580), Expect = 0.000e+0
Identity = 552/1187 (46.50%), Postives = 758/1187 (63.86%), Query Frame = 0

Query:   35 VGLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAE--HGLERWETVYVLLLWLSMLVLIPFDMGRFDS------GAEVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVAR----MGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAGEDADADYDIPDD--IEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYLKD----PKTSTLLVGKRDKIFDEYLHEL-GNSELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGYG---PDGLASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPEL-VPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDMS--LAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEI--LRPMRNNLCDLMGVPAPAIIKKPA 1194
            VG G  LE F+E Q + + I  VE   N  +++  E   ++FT  +D YQEQPHLIDP     + +++ ++R N   P A     +  A      I K+R  K  +RQFPHEV DVEPVL+ + A+     + WET Y+LLLWLS++ +IPFDM R DS      G +   +  RI    + YL    + ++AAA LA+KF TRP++  E L  FLDW L V  +     V       G L  LA ++K G+R +LL++   +L  +  LEL+ + N LLR+ ++KVIQR GL FL ARVA+WRYQRGNRSL+ NL        GS P   N    G+D + + +      +E+VIE LL GL+DKDT++RWSAAKGIGR+T RLP ELAD+VV S+L LF+L+E++ AWHGGCL LAELGRRGLLLP+RL  VV VVL++L YDE++G FSVG+++RDAACYVCWAFARAY+P  +  +V +++ AL+ V+IFDRE+N RRAA+AAFQENVGRQG FP GIDILT ADY+AVG+R++ YLDLSV+VAQF +Y +ALI+HL++ KV+HWD ++R+L+A  LHNL+    + M   VLPKLL L  G DLF +HGAI+A+  +  AL+K  E +   +   L   ++  +K I   L E    R  GGDL+R+A+T  I   SLA+ P H D  + LW  +++  L + +  IQ  A +A+P F  EY  +    PK       K++ + ++Y+ EL  N E  R+GFSLALGA P+F L+G+   IL+ LF+   IT   + W EARRDAI A+T I   +G G    D L ++ V  +Y  LL   +DYTLD RGD+GAWVREA+M  L  +T  L+  +P+L   +IC R  M  L QQ  EKIDRTR  A ++F+  L HE  +P IP  ++L+  FP ++ +    W   S TF +FT+LL L+ Y   VL+GL VSVGGLTE LV ++S +L   +  +   L +  HF   +L++ +  Q+ DRV+ P +K LDQLL+ G  +    ++   F   ++ LV+AE+ + GDP KL+ S DV C LLQ    +  KKAL QLSIFLCHK+PR+RK TANKL+EAL+T+ +  +VPEE+ D +  +LS+  WD  +E+  +RP+RNNLCDLMGVP P ++KK A
Sbjct:   14 VGKGHVLEEFKEIQELRSLIASVENTYN--DQVAREASCERFTFIIDQYQEQPHLIDPHLESLMMQIIDIVR-NLKFPAA----LKQEAYKYLYLITKMRGFKTVIRQFPHEVVDVEPVLSAISAQDPKDFQTWETRYMLLLWLSVVCMIPFDMVRLDSNIPSEDGEKKQPVMDRILNTAQIYLTVNDKCRDAAAFLAAKFCTRPDVRREKLPEFLDWSLQVLHNANLETVIGNNEVSGVLTMLALLFKNGKREDLLQYAETVLQVVEKLELRKNNNTLLRKAAVKVIQRLGLTFLKARVASWRYQRGNRSLMDNL-----QKGGSGPTTMN---KGQDEEEEEEYDIPEELEDVIEYLLGGLKDKDTIVRWSAAKGIGRVTGRLPLELADEVVGSVLQLFTLQETEGAWHGGCLTLAELGRRGLLLPQRLPDVVPVVLRSLEYDERRGDFSVGANVRDAACYVCWAFARAYEPGDVAKYVKDLSNALVKVSIFDREINVRRAAAAAFQENVGRQGIFPHGIDILTHADYFAVGNRSHCYLDLSVYVAQFPEYTIALINHLVDIKVSHWDSSVRELSARGLHNLTPKAPEYMAEHVLPKLLPLTTGMDLFARHGAILAVAEITHALAKVAEDQNKSITDLLSEGVIEGMKNIINQLNEGRLFRGWGGDLMREAMTCLIKKLSLARLPFHGDPVLDLWQELISGCLQHVEQNIQVAAAEAIPAFYREYYMEGDGSPKKQ-----KQEWVVNKYIQELKSNVETTRKGFSLALGALPKFFLQGQVEVILKSLFEAAEITEKEKKWVEARRDAIQAITKICTAVGVGDSPEDSLCAANVPSVYNSLLTAMKDYTLDSRGDVGAWVREASMTGLSEVTSLLVRTAPDLFTADICGRA-MQCLVQQCCEKIDRTRSHAGQIFSQFLYHEPEIPHIPHREKLQEIFPKSV-IEGINWGAPSDTFCLFTQLLALDTYLYHVLLGLTVSVGGLTESLVIHSSSSLHAYLRSIGKDLQKMTHFGEVLLQIFKDYQREDRVSFPMLKMLDQLLSKGCFDVFANDDQHPFPPSVMELVRAEVLRCGDPHKLIASVDVYCDLLQFEGDVR-KKALQQLSIFLCHKYPRVRKATANKLYEALVTYDE--LVPEESLDEITTVLSEFTWDDELELDNIRPVRNNLCDLMGVPRP-VLKKVA 1174          

BLAST of tubulin-specific chaperone d vs. nr
Match: gi|1207947039|ref|XP_021363386.1| (tubulin-specific chaperone D-like [Mizuhopecten yessoensis] >gi|1205895783|gb|OWF45618.1| Tubulin-specific chaperone D [Mizuhopecten yessoensis])

HSP 1 Score: 996.112 bits (2574), Expect = 0.000e+0
Identity = 547/1184 (46.20%), Postives = 743/1184 (62.75%), Query Frame = 0

Query:   36 GLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAE--HGLERWETVYVLLLWLSMLVLIPFDMGRFDSGAEVVSLRRR------IDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVARM----GALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAG-EDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHELGNS-ELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGYGPDG-----LASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELV-PEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLAL--FQEVDDMSLAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDK-SIEILRPMRNNLCDLMGVPAPAIIKKPAPA 1196
            G G  LE F+E +  +  I G++   N  +++  E   ++FT   D YQEQPHLID      + ++L ++R+    P        H A      I K+R  K+ VRQFPHEV+DVEPVLAL+  +     + WE+ Y+L+LWLSM+ +IPFDM R DS A  VS  RR      I  V R YL    + ++AAA L S+F TRP++  E L  FLDW L++  +     +  M    G L   A ++K G+R +LL + P +L ++  L+++D  N ++R+  +KVIQR GL FL  RVAAWRYQRG+RSL  NL        G +   Q+  +      D +YDIP+++EEV+E+LLSGL+DKDTV+RWSAAKGIGR+T RLPRELA++VV S+L LF+ +E+D AWHGGCLALAELGRRGLLLPERL  VV VVLK+L YD+K+G+FSVG+H+RDAACYVCWAFARAY+P  + PHV++IA AL+  +I+DREVN RRAA+AAFQENVGRQGTFP GIDILT  DY+AVG+R++ YLDLSVFVAQ+  Y   LIDHL+  K++HWD  +R+LTA ALH L+  + + M   VLP L+ L  G DLF +HG I+A+  +  AL K + ++   +   +  D++  ++ IA  L +    R  GG+L+R+A+T  I   SL+K P H D  + LW  I+ E L   +P IQE A+ A+P F  EY  D K    +  K++K+   YL EL +  E+ R+G+SLA+GA P+F++K +   IL  L     +T   + WAE+RRDA+ A+T + +++G    G     L S  + ++Y       QDYTLD RGD+GAW REAAM  L  IT  +++    L+ PEI  + +   L QQA EKIDRTR  A  +F  LL H   +P IP  Q L+  FP   +     W   S TFP+FT+LL    Y   VL+GL VSVGGLTE LV Y+S +L  +    +   AQ   F   ++++  ++ K DRV+LP +K L+QLL+ G      ENEN  F+  +L L K EI++SGDPQKL+ S DV C LLQ    +  +KAL QL + + HK+PRIRK TANKL+EAL+T+ D  + PEE  D    I+S+T WD   I  +RP RN LCDL+ +P P +  KP  A
Sbjct:   20 GKGHVLEEFKEIEEFKGLIAGLKNIYN--DQIALEVSCERFTYITDDYQEQPHLIDSHLESLMTQILDIVRDVKSPP-----ALIHLAFKYLYLITKMRGYKIIVRQFPHEVADVEPVLALISQQDPEDHQTWESRYMLILWLSMVCMIPFDMVRLDSNAVSVSGERRRPVMERILDVARMYLTQNDKCRDAAAYLTSRFLTRPDVKKERLPDFLDWTLNILMNSDMTTMRGMTQVTGILTMTALLFKHGKREDLLEYAPVVLTKVNDLKVRDSNNCVIRKNGIKVIQRLGLTFLKNRVAAWRYQRGSRSLADNL------KQGYQHEKQSTVVTNITQEDEEYDIPEEVEEVMEQLLSGLKDKDTVVRWSAAKGIGRVTGRLPRELANEVVGSVLELFTFQETDGAWHGGCLALAELGRRGLLLPERLPDVVPVVLKSLEYDDKRGNFSVGAHVRDAACYVCWAFARAYEPQEITPHVNDIATALIKASIYDREVNVRRAAAAAFQENVGRQGTFPHGIDILTTVDYFAVGNRSHCYLDLSVFVAQYPQYIQGLIDHLVHIKISHWDSAVRELTAKALHRLTPKSPEYMAKTVLPLLMPLTTGPDLFLRHGTILAVAEITHALGKLVAEQNRAITDVIDADVIEGLRRIAKKLHDVELFRGMGGELMRKAVTCLIEKLSLSKLPYHGDPIIELWQGIIDECLHYVEPEIQEAAVAAIPAFFQEYYSDSKGGA-IPDKQEKVISRYLEELKSPVEVSRKGYSLAIGALPKFIMKNKLKGILSGLMGATRVTEREKKWAESRRDAVKAITGVCKSVGVDKQGDPTQVLCSQNIPEVYDTCFQAMQDYTLDSRGDVGAWAREAAMTGLHEITTMIVNTDASLITPEIS-KNVFCCLVQQACEKIDRTRAHAGNIFKCLLYHRPEIPHIPHRQVLEEVFPRE-EAEKVNWAAPSDTFPLFTKLLKCPAYQYSVLLGLTVSVGGLTESLVIYSSGSLNAYLRTINKDSAQMSQFVDVLMKIFESHIKIDRVSLPMLKMLNQLLSKGCFNVFAENENQEFAQKILELSKKEISRSGDPQKLIASADVFCELLQFCAEVK-RKALFQLLVLMGHKYPRIRKATANKLYEALVTYDD--VAPEEGLDEAMVIISETTWDNDKINEVRPFRNQLCDLLEIPQPKMKAKPDDA 1184          

BLAST of tubulin-specific chaperone d vs. nr
Match: gi|1229193484|ref|XP_022110714.1| (tubulin-specific chaperone D-like isoform X2 [Acanthaster planci])

HSP 1 Score: 988.408 bits (2554), Expect = 0.000e+0
Identity = 546/1158 (47.15%), Postives = 743/1158 (64.16%), Query Frame = 0

Query:   65 NRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAEH--GLERWETVYVLLLWLSMLVLIPFDMGRFDSGAEVVSLRR-------RIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVARM----GALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAEL-APAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAEN---LSNSDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHELGNS--ELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGYGPDG-----LASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDM-SLAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEILRPMRNNLCDLMGVPAPAIIKKPAPAT 1197
            +++  E   +KFT+ +D YQEQPHL+DP     +  LL + R++   P        H A      + K R  K  VR  PHEV+D+EP LALL  +     E WET Y+LLLW+S++ +IPFDM R DS A +    R       RI    + YL    ++++AAA++ +KF TR ++  ELL  FLDWCL   ++     +  M    G L  LA+++K G+R +L+ + P +L R++A  L D  N  L +L++K+ QR GL FL AR AAWRYQRG+RSL  NL +++ AP A    G  +  +A ED D +YD+P++IEEVIE+LL GLRD+ T++RWSAAKG+GR+T RLPRELAD VVES+L LF L E D AWHGGCLALAELGRRGLLLPERL  VV VVLKAL YDEKKGS SVG+H+RDAACYVCWAFARAYDP  ++P+++++A AL+  T+FDREVNCRRAASAAFQENVGRQGTFP GIDILT AD+ AVG+R N +L +S ++A F +Y + LIDHL + K+ HWD  IR+LT+ ALHNL+    + M   VLP L+ LA G DLF +HGA++A   V  AL K   +    +   LGP+ V+ +K I   +VE    R   G+LLR A+  +I   SL+K   H DD + +W  +L +N   L  ++  IQ  A+KA+  F  EY + P   T+L G +DK+ D YLHEL  S  E+ R G +++LG  P+F+L+ +   +L+ L +   I+  TE +AE+RRD I A+  I +T+G   DG     +    +  +Y  LLG  +DYT D RGD+GAWVREA+M SL  +T  ++   P L+    +++LM  LAQQA EKIDRTR  A      LL HE  VP IP  ++L   FP + D+    W+  S++FP  TRLL+L  Y   VL+GL VSVGGLTE LVK++S +L   +  + SL   + F  AML V +  +K DRVTLP MK ++ LL++G LE   ENE+  F + LL  +K EIAK G+PQKL+ S DV C+++     +  +K L Q+ +FLCHK+P++RK +A KL+E LL + D  +VPE++ + +  ILS+T WD+S+  +RP+RN LCDLM +P P +  K A AT
Sbjct:   53 DQISVELAVEKFTVIVDKYQEQPHLMDPHLEGILLPLLDIARQSGIDPPLS-----HLAFKFMYLLTKARGFKTIVRILPHEVADLEPALALLTRQDPADFETWETRYMLLLWMSIICMIPFDMARLDSNAPLDDEGRKREPTMMRIINAAKIYLRVCDKSRDAAALMIAKFLTRHDVKQELLPSFLDWCLRSLTETNYDTMTGMTFLSGLLYTLASLFKIGKREDLVEYAPVVLERLVACHLFDSNNTQLHKLAIKLTQRLGLTFLCARTAAWRYQRGSRSLAANLNSQVSAPLA---VGTGSSQIATEDDDEEYDVPEEIEEVIEQLLVGLRDRSTIVRWSAAKGVGRMTGRLPRELADQVVESVLELFCLGELDGAWHGGCLALAELGRRGLLLPERLPEVVPVVLKALAYDEKKGSCSVGAHVRDAACYVCWAFARAYDPQDIKPYINKLASALVITTVFDREVNCRRAASAAFQENVGRQGTFPHGIDILTTADFLAVGNRTNVFLHISCYIADFEEYTLPLIDHLAKIKIGHWDGAIRELTSQALHNLTPKALNYMIETVLPSLIPLATGIDLFTRHGALLAAAEVTHALYKHAIETNRTVSDVLGPESVAGLKQIVKTMVEAKMFRGVTGELLRPAVCVFIEKMSLSKLRCHGDDILEVWQFVLDDNLIGLQRTEVDIQSYAVKALGAFYMEYYQQPD-GTVLPGLQDKVLDRYLHELATSQQEMGRMGAAMSLGRQPKFMLETKLMKVLQGLAKASSISSQTEKFAESRRDVIKAIARICKTVGIKADGDPTCLVCGDNIGMVYSALLGAMKDYTTDSRGDVGAWVREASMTSLRELTSLVVKTEPTLITADTIQQLMQCLAQQASEKIDRTRACAGAAILGLLHHEPQVPYIPCREQLLEIFPRS-DLQELNWSAPSESFPKMTRLLDLPVYRHHVLLGLTVSVGGLTESLVKHSSQSLMAYLKGLSSLTDLEGFVSAMLDVFQEYEKVDRVTLPMMKMINILLSAGSLEIFAENEDHQFPVQLLDRMKKEIAKCGEPQKLLASVDVFCSMI-VFPGVTRRKCLFQVLMFLCHKYPKVRKTSAEKLYEMLLMYDD--VVPEDSQEEILTILSETKWDESVLTVRPIRNQLCDLMNIPRPVL--KSAKAT 1195          

BLAST of tubulin-specific chaperone d vs. nr
Match: gi|1229193482|ref|XP_022110713.1| (tubulin-specific chaperone D-like isoform X1 [Acanthaster planci])

HSP 1 Score: 986.097 bits (2548), Expect = 0.000e+0
Identity = 542/1150 (47.13%), Postives = 739/1150 (64.26%), Query Frame = 0

Query:   65 NRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAEH--GLERWETVYVLLLWLSMLVLIPFDMGRFDSGAEVVSLRR-------RIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVARM----GALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAEL-APAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAEN---LSNSDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHELGNS--ELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGYGPDG-----LASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDM-SLAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEILRPMRNNLCDLMGVPAPAI 1189
            +++  E   +KFT+ +D YQEQPHL+DP     +  LL + R++   P        H A      + K R  K  VR  PHEV+D+EP LALL  +     E WET Y+LLLW+S++ +IPFDM R DS A +    R       RI    + YL    ++++AAA++ +KF TR ++  ELL  FLDWCL   ++     +  M    G L  LA+++K G+R +L+ + P +L R++A  L D  N  L +L++K+ QR GL FL AR AAWRYQRG+RSL  NL +++ AP A    G  +  +A ED D +YD+P++IEEVIE+LL GLRD+ T++RWSAAKG+GR+T RLPRELAD VVES+L LF L E D AWHGGCLALAELGRRGLLLPERL  VV VVLKAL YDEKKGS SVG+H+RDAACYVCWAFARAYDP  ++P+++++A AL+  T+FDREVNCRRAASAAFQENVGRQGTFP GIDILT AD+ AVG+R N +L +S ++A F +Y + LIDHL + K+ HWD  IR+LT+ ALHNL+    + M   VLP L+ LA G DLF +HGA++A   V  AL K   +    +   LGP+ V+ +K I   +VE    R   G+LLR A+  +I   SL+K   H DD + +W  +L +N   L  ++  IQ  A+KA+  F  EY + P   T+L G +DK+ D YLHEL  S  E+ R G +++LG  P+F+L+ +   +L+ L +   I+  TE +AE+RRD I A+  I +T+G   DG     +    +  +Y  LLG  +DYT D RGD+GAWVREA+M SL  +T  ++   P L+    +++LM  LAQQA EKIDRTR  A      LL HE  VP IP  ++L   FP + D+    W+  S++FP  TRLL+L  Y   VL+GL VSVGGLTE LVK++S +L   +  + SL   + F  AML V +  +K DRVTLP MK ++ LL++G LE   ENE+  F + LL  +K EIAK G+PQKL+ S DV C+++     +  +K L Q+ +FLCHK+P++RK +A KL+E LL + D  +VPE++ + +  ILS+T WD+S+  +RP+RN LCDLM +P P +
Sbjct:   53 DQISVELAVEKFTVIVDKYQEQPHLMDPHLEGILLPLLDIARQSGIDPPLS-----HLAFKFMYLLTKARGFKTIVRILPHEVADLEPALALLTRQDPADFETWETRYMLLLWMSIICMIPFDMARLDSNAPLDDEGRKREPTMMRIINAAKIYLRVCDKSRDAAALMIAKFLTRHDVKQELLPSFLDWCLRSLTETNYDTMTGMTFLSGLLYTLASLFKIGKREDLVEYAPVVLERLVACHLFDSNNTQLHKLAIKLTQRLGLTFLCARTAAWRYQRGSRSLAANLNSQVSAPLA---VGTGSSQIATEDDDEEYDVPEEIEEVIEQLLVGLRDRSTIVRWSAAKGVGRMTGRLPRELADQVVESVLELFCLGELDGAWHGGCLALAELGRRGLLLPERLPEVVPVVLKALAYDEKKGSCSVGAHVRDAACYVCWAFARAYDPQDIKPYINKLASALVITTVFDREVNCRRAASAAFQENVGRQGTFPHGIDILTTADFLAVGNRTNVFLHISCYIADFEEYTLPLIDHLAKIKIGHWDGAIRELTSQALHNLTPKALNYMIETVLPSLIPLATGIDLFTRHGALLAAAEVTHALYKHAIETNRTVSDVLGPESVAGLKQIVKTMVEAKMFRGVTGELLRPAVCVFIEKMSLSKLRCHGDDILEVWQFVLDDNLIGLQRTEVDIQSYAVKALGAFYMEYYQQPD-GTVLPGLQDKVLDRYLHELATSQQEMGRMGAAMSLGRQPKFMLETKLMKVLQGLAKASSISSQTEKFAESRRDVIKAIARICKTVGIKADGDPTCLVCGDNIGMVYSALLGAMKDYTTDSRGDVGAWVREASMTSLRELTSLVVKTEPTLITADTIQQLMQCLAQQASEKIDRTRACAGAAILGLLHHEPQVPYIPCREQLLEIFPRS-DLQELNWSAPSESFPKMTRLLDLPVYRHHVLLGLTVSVGGLTESLVKHSSQSLMAYLKGLSSLTDLEGFVSAMLDVFQEYEKVDRVTLPMMKMINILLSAGSLEIFAENEDHQFPVQLLDRMKKEIAKCGEPQKLLASVDVFCSMI-VFPGVTRRKCLFQVLMFLCHKYPKVRKTSAEKLYEMLLMYDD--VVPEDSQEEILTILSETKWDESVLTVRPIRNQLCDLMNIPRPVL 1189          

BLAST of tubulin-specific chaperone d vs. nr
Match: gi|1351483666|ref|XP_013417607.2| (tubulin-specific chaperone D-like [Lingula anatina])

HSP 1 Score: 984.941 bits (2545), Expect = 0.000e+0
Identity = 547/1190 (45.97%), Postives = 756/1190 (63.53%), Query Frame = 0

Query:   26 EPEDPYTNAVGLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAEHGL--ERWETVYVLLLWLSMLVLIPFDMGRFDSGAEVVS------LRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLS--VASDPA--QPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNL-LAELAPAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHELGN-SELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTE-TWAEARRDAILALTSIAQTLGYGPDGLASSTVV-----QLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDMS--LAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEILRPMRNNLCDLMGVPAPAIIKKP 1193
            E E    +A+G G   E F+EA  V   I  ++      +++  E   ++FT+ +D YQEQPHL+DP   + + +L+ ++RE+     A  +   H        I KVR  KV VR  PHEV+DVEPVLALL  ++ +  E WET YVL++WLS++ +IPFDM R DS  +  +      +  RI  V + YL    +A +AAA  A++F TRP++  + L   +DW +   V +D +  Q   A  G L  LAA++K G+R +++ F P +L ++    L++  N +LR+L++K++QR GL FL  RVAAWRYQRG+RSL  NL L  L PA  ++P   +  L  ++ +      D++EEVIE+LL+GL+DKDT++RWSAAKG+GR+T RLP+ELAD+VV S+L LFSLRESDAAWHGGCLALAELGRRGLLLPERL AVV +VLKAL YDEKKG FSVG+H+RDAACYVCWAFARAYDP+ ++ +V+ IAC+L+  ++FDREVN RRAASAAFQENVGRQ TFP GIDILT ADY+AVG+R   YL+LSVFVAQF +Y  ALIDHL + KVN WD  IR+L A AL+ L+  + + M + +LP LL    G DL  +HG I+A+  +   L++  ++ G  +   + P ++  +K IA  L +    R  GG+L+R+A++  I   S+AK P H+D  +  W  I+ + L + +P IQ  A  A+P F  EY   P  S      ++KI   YL EL +  E+ R GFS A+G  P+F+L+G+   +L+ L     +  D + T+AEAR++A+ A+T I  T+G   DG   S +      + +  L     DYTLD RGD+GAWVREAAM +LC +    +  +P L+     + L   L QQA EKIDRTR LA   F  LL H   +P IP  +EL   FP   ++    W V S TFP  T+LL++  Y   VL+G+ VSVGGLTE LVK++  +L   +  +S      Q+F   ++++ +  QK DRV+LP +K LDQLLT+G  + I E+E++ F MDLL L K EI++SG  QKL+ S D+ C LLQ   S   KKAL+QL +FLCH++P IRK TANK++EAL+T+ +  + P+EN D +  +LS+T WD  +E LRPMRN LC L  VP P + K+P
Sbjct:   11 EQERQNLDALGEGLIHEEFKEADEVSNIISTLKDIYT--DQIAVEMALERFTVIMDSYQEQPHLLDPHLEKLLGQLIEIVRES-----AVPMPVIHLVFKCLYLITKVRGYKVIVRLMPHEVADVEPVLALLAQQNPMDHETWETRYVLMIWLSIVCMIPFDMVRLDSNIKTATGEVKKPIMDRILDVVKIYLNVNDKALDAAAYCAARFMTRPDVKKKKLPELMDWLVQGLVKADTSKMQGVTAMRGELATLAAIFKHGKREDVIDFAPIVLEKLGECNLREVNNTILRKLAIKIVQRLGLTFLKFRVAAWRYQRGSRSLAENLALKGLTPAV-AKPTMVHTKLEDDEEEEFDIP-DEMEEVIEQLLNGLKDKDTIVRWSAAKGVGRVTGRLPQELADEVVGSVLELFSLRESDAAWHGGCLALAELGRRGLLLPERLSAVVPIVLKALAYDEKKGDFSVGAHVRDAACYVCWAFARAYDPEEIKIYVNNIACSLVIASVFDREVNVRRAASAAFQENVGRQCTFPHGIDILTTADYHAVGNRTYCYLELSVFVAQFPEYTKALIDHLAQVKVNRWDSVIRELAAQALYKLTPCSPEYMANTILPALLPQTTGIDLNTRHGTILAVAHITQGLAEWAQENGKKITDVVDPSIIDGLKNIAKTLQDAKLFRGLGGELMRRAVSCLIEKLSIAKMPYHNDTIIDCWQEIIDDCLRHIEPSIQAAATSAIPAFFSEYHSLPDGSP-KPEFQEKILTRYLEELKSIVEITRMGFSQAIGQFPKFMLQGKLKPVLQSLNHAATVMTDKDKTFAEARKEALNAMTCIFHTVGVETDGSPHSVICKENIHEQFDILFVAMGDYTLDSRGDVGAWVREAAMCALCELITLTVKVNPTLITAEITKHLFCCLVQQASEKIDRTRALAGETFMKLLYHSPEIPNIPHKEELLQIFPWE-EIKGLNWAVPSDTFPRLTKLLSVPVYCYSVLLGMTVSVGGLTESLVKHSGTSLIGYLKSLSNDAGAVQNFADVLIKIFKDYQKVDRVSLPMLKMLDQLLTNGCFQVICEDESNTFPMDLLTLCKQEISRSGAVQKLLASIDIFCTLLQ-FSSETKKKALIQLMVFLCHRYPIIRKTTANKVYEALVTYEE--VGPDENLDELLTLLSETSWDDEVEKLRPMRNQLCSLFNVPPPVMKKQP 1186          

BLAST of tubulin-specific chaperone d vs. nr
Match: gi|1339038623|ref|XP_023725677.1| (tubulin-specific chaperone D [Cryptotermes secundus] >gi|1330936493|gb|PNF44135.1| Tubulin-specific chaperone D [Cryptotermes secundus])

HSP 1 Score: 975.696 bits (2521), Expect = 0.000e+0
Identity = 521/1185 (43.97%), Postives = 733/1185 (61.86%), Query Frame = 0

Query:   28 EDPYTNAVGLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAEH--GLERWETVYVLLLWLSMLVLIPFDMGRFDSGAEVVSLRRRIDA---VTRQYLVA--TVRAQN----AAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHEL-GNSELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGYGPDGLASSTVVQL---YQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEV-----DDMSLAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEILRPMRNNLCDLMGVPAPAIIKK 1192
            E+   + +GLGCALE F E + V   IE +      ++    EW YQ+F+  L+ YQEQPHL+DP     + +L+ ++R         S+  +HA       I+KVR  K A+   PHEVSD+EPVL LLE +    ++ WET  VLL+WLS++V++P  M R DS       RRR +    V  + L+A  T    N     AA L+S F TR ++    L  FLDW  +          A+ G+L A+A++ K G+R +LL     LL  I+A + +D+P+ L+R+  +KVIQR GL FL  RVAAWRY+RG+RSL  NL A         P  +      E+ + D++IPDD+EEVIEELL GLRD +TV+RWS+AKGIGR+T RLP+ELAD++V S+L LFS RES+ AWHGGCLALAELGRRGLLLP+RL  VV ++LKAL+YDE+KG  +VG+HIRD ACYVCWAFARAYDP  L+P V +IA ALL VT FDR++ CRRAASAAFQENVGRQGTFP GI+I+T ADY +VG R N +L++SV++AQF +Y + LI+HLI++KV HWD+ IR+LTA ALHNL+      M   VLP LL      DL   HG+++AI  +  AL+   E+    +   +G  ++  ++ + P   ++   R  GG+L++QA +  I  CSLA  P H+   +  W ++L E LS+    I+ KA  A+  F  +Y +   +  +   + D +   Y  +L  NS+  R GFSLALG+CP+F+       ++  L QC  IT  T  W+E RRDA+ AL SI  T+  G + L S     L   Y CLL G  +YT+D RGDIG+WVREAA+  L  +T  ++   P L+    V  +M S+AQQAVEKIDR R  A ++FT+LL     +P IP H EL   FP         W   S TFP FT+LL    +T  VL+GLV SVGGL+E L K +S +LF  +     D++ L   +  C  ++ + + +   DR+T+P ++FLD+L +SG +  +LEN  S F+ D+L  VK EI ++ +  KL  S +++C L+Q +  ++ +++LVQL IFLC++   +R+ TA++L+EALL + + S++P E  D V  IL+DT+W + ++I++P RN +C+LMGVP P  + +
Sbjct:    9 EENCDDNIGLGCALEMFSEHEEVITMIENLRTV--YSSERSAEWAYQRFSCILNRYQEQPHLLDPHLDSILNKLISIVRNGES-----SIELKHATFKYLYIIMKVRGYKTAMTHLPHEVSDIEPVLQLLENQEPSDVKTWETRCVLLVWLSIIVMMPVHMSRLDSFKP--EDRRRDEGRKTVMERILIACKTYAFSNDQFGVAAFLSSHFLTRSDVKETHLPVFLDWACNKIVSSDSTLFAQCGSLAAVASILKHGKREDLLSHAHSLLKCILASKYRDNPSILIRKFGVKVIQRIGLTFLKVRVAAWRYERGSRSLAANLTAVHPLPVNPVPQKE------EEMNEDFEIPDDVEEVIEELLQGLRDSNTVVRWSSAKGIGRVTGRLPKELADEIVGSVLELFSPRESNGAWHGGCLALAELGRRGLLLPQRLGEVVPLILKALMYDEQKGYGAVGAHIRDGACYVCWAFARAYDPAILQPFVKDIAGALLVVTAFDRDLKCRRAASAAFQENVGRQGTFPHGIEIVTAADYVSVGVRTNAFLNVSVYIAQFEEYTLLLINHLIDRKVGHWDIVIRELTAKALHNLTPKASTYMAETVLPVLLDKTSTVDLNTCHGSVLAIAEILHALAIVAEQSDCTVQDVIGVKIMEQVRDLVPTFRQRQQFRGLGGELMKQACSLLIEKCSLAVMPFHNHPIISDWQSLLDECLSHEVSNIRSKAASALSPFFTQYYQQEHSHGVNTARHDAVIATYTEQLAANSQTIRMGFSLALGSCPKFMFDDHLHMVIPALIQCSKITESTVRWSETRRDALKALISICSTVS-GENDLGSELTKHLDDIYGCLLEGLAEYTMDGRGDIGSWVREAAITGLQVLTCLVVKSDPSLLTSELVTRIMVSVAQQAVEKIDRIRAHAGKVFTSLLHSAPAIPNIPHHAELVQIFPAAACEKEINWIAASNTFPRFTQLLAFRPFTYNVLLGLVSSVGGLSESLTKDSSASLFSYLKAHHGDNVEL---ERLCETIVEIFQHSHFVDRITVPLLEFLDRLFSSGCVRSVLENSQSTFASDVLHHVKLEIGRTRNVSKLTGSVNILCHLVQVNGEVS-RRSLVQLCIFLCNRVMFVRRTTASRLYEALLLYGEYSVIPAEKLDEVMTILNDTNWQEDVDIVKPTRNKICNLMGVPVPGTVAR 1173          

BLAST of tubulin-specific chaperone d vs. nr
Match: gi|1139856381|ref|XP_011423542.2| (PREDICTED: tubulin-specific chaperone D [Crassostrea gigas])

HSP 1 Score: 969.533 bits (2505), Expect = 0.000e+0
Identity = 551/1182 (46.62%), Postives = 753/1182 (63.71%), Query Frame = 0

Query:   36 GLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAE--HGLERWETVYVLLLWLSMLVLIPFDMGRFDS------GAEVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVAR----MGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDK---IFDEYLHEL-GNSELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGYG--PDG-LASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPEL-VPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALFQEVDDMS--LAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEI--LRPMRNNLCDLMGVPAPAIIKKP 1193
            G G  LE F++ Q V++ I  ++   N  +++  E   ++FT  +D YQEQPHLIDP     + +++ ++R NP  P A     +  A      I K+R  K+ +RQFPHEV+DVEPVL+ +  +     + WET Y+L+LW+S++ +IPFDM R DS      G +   +  RI  + R YL    + ++AAA LA+KF TRP++  E L  FLDW L V  +     +       G L  LA ++K G+R +LL F   +L  +  LEL  + N LLR+ ++KVIQR GL FL ARVAAWRYQRGNRSL+ NLL       G+     N   A E+ + +YDIP+++E+VIE LL GL+DKDT++RWSAAKGIGR+T RLP ELAD+VV S+L LF+L E++ AWHGGCL LAELGRRGLLLP+RL  VV V+LK+L YDE++G FSVG+++RDAACYV WAFARAY+P  +  +V +IA AL+ V+IFDREVN RRAA+AAFQENVGRQG FP GIDILT ADY+AVG+R++ +LDLSV++AQF +Y VALI+HL + KV+HWD ++R+L+A  LHNL+    +LM  +VLP+L+ L  G DLF +HGAI+A+  +  AL+K  E +   +   L  +++  +K I   L      R  GGDL+R+A+T  I   SLAK P H D  + LW  +++  L + +  +Q  A +A+P F  EY  +   S     K+DK   +  +Y  EL  N E  R+GFSLALGA P+F L+G+   +   LF+   I    + W EARRDAI A+T+I   +G G  P G L +  V  +Y  LL    DYTLD RGD+GAWVREA+M  L  +T  L++  PEL   +IC R  M  L QQ  EKIDRTR  A ++F+  L HE  +P IP  +EL   FP + ++    W   S TF  FT+LL LE Y   VL+GL VSVGGLTE LV ++S +L   +  +S  L +  HF   +L++ +  QK DRV+ P +K LDQLL+ G  +         F + ++ +VK E+ +SGDP KL+ S +V C LLQ    +  KKAL QL++FLCHK+PR+RK TANKL+EAL+T+ +  IVPE++ D +   LS+  WD  IE+  +RP+RNNLCDLM VP P + K+P
Sbjct:   13 GKGHVLEEFKDLQEVKSLIASLKNTYN--DQVAREASCERFTFIIDEYQEQPHLIDPHLESLVMQIIDIVR-NPSHPPA----LKQEAYKYLYLITKMRGFKIVIRQFPHEVADVEPVLSAISQQEPQDFQTWETRYMLILWVSVVCMIPFDMVRLDSNIPSSGGEKKQPVMDRILNIARTYLTVNDKCRDAAAFLAAKFCTRPDVRKEKLPEFLDWSLQVLHNANMDTIVGNNQVSGVLTMLALLFKNGKREDLLEFAAPVLQVVEKLELNKNNNTLLRKAAVKVIQRLGLTFLKARVAAWRYQRGNRSLLDNLLKR-----GNVQPTVNQTQAVEEEEEEYDIPEELEDVIEYLLGGLKDKDTIVRWSAAKGIGRVTGRLPMELADEVVGSVLQLFTLLETEGAWHGGCLTLAELGRRGLLLPQRLPDVVPVMLKSLEYDERRGDFSVGANVRDAACYVSWAFARAYNPGDMAKYVKDIANALVKVSIFDREVNVRRAAAAAFQENVGRQGIFPHGIDILTHADYFAVGNRSHCFLDLSVYIAQFPEYTVALINHLADVKVSHWDSSMRELSARGLHNLTPKAPELMAEQVLPRLIPLTTGMDLFARHGAILAVAEITHALAKVAEDKNKSITDVLSEEVIEGMKNIIKLLNTANLFRGWGGDLMRRAMTCLIEKLSLAKLPFHGDPVLDLWQDLISGCLQHVEQDVQIAAAQAIPAFYREYFMEGDGSP----KKDKQEWVVRKYTQELKSNMETTRKGFSLALGALPKFFLQGQLEVVFTSLFEAAEIKEKEKKWVEARRDAIKAITNICTAVGVGNSPQGSLCADNVPAVYNALLRAMTDYTLDSRGDVGAWVREASMTGLQEVTSLLVNNKPELFTADICTRA-MQCLVQQCCEKIDRTRSHAGQIFSVFLSHEPEIPHIPHREELHQIFPKS-ELGDINWGAPSDTFCRFTQLLGLETYMYNVLLGLTVSVGGLTESLVIHSSSSLHVYLRSISKDLTKMTHFGEVLLQIFKDYQKVDRVSFPMLKMLDQLLSKGCFDVFSGVNQHQFPLSVIDIVKTEVTRSGDPHKLIASVEVYCGLLQFEGEVR-KKALQQLTMFLCHKYPRVRKATANKLYEALVTYDE--IVPEDSLDEITTTLSEFTWDDEIEVESIRPVRNNLCDLMNVPRPVLKKRP 1173          

BLAST of tubulin-specific chaperone d vs. nr
Match: gi|1080048853|ref|XP_018565799.1| (tubulin-specific chaperone D [Anoplophora glabripennis])

HSP 1 Score: 959.518 bits (2479), Expect = 0.000e+0
Identity = 515/1171 (43.98%), Postives = 734/1171 (62.68%), Query Frame = 0

Query:   36 GLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAE--HGLERWETVYVLLLWLSMLVLIPFDMGRFDSGAEVVSLRR----RIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAELAPAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHEL--GNSELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTLGY--GPDGLASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALF----QEVDDMSLAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEILRPMRNNLCDLMGVPAPAIIKK 1192
            GLGCALE F E + +   I+ ++     A+R   E  Y+ F+  L  Y EQPHL+D      +++ ++++R N    E      +H A      ++ VR  KV VR  PHEV+D EPVL LLE++  +  E W T Y+LLLWLS++V+IPF M RFD   E  + ++    RI  V + Y V   + ++AAA L+ KF TR ++  + L  FLDW + ++         + G L  +A + K G+R +LL    +LL  II  E K++  + +++L  K++QR GL FLP RVAAWRY+RGNRSL  NL A      GS P   N D   E+ D D D+PD++EEVI++L+ GLR  D+++RW+AAKG+GR+T RLP++LAD+VV S L LFS RESD AWHGGCLALAELGRRGLLLPERL  VV VVLKALVYDE +G  SVGSHIRDAACYVCW+FARAY+   L PHV++IA  LL VT FD+E+NCRRAASAAFQENVGRQG+FP GIDILT AD++ V  RNN YL++SV++AQF +Y + LIDHL+ +KV+HWD  IR+L++ ALHNL+    + M   VLP L    +  DL  +HGA +AIG V  ALSK  +++ +D+   L  D++   + + P   E+L  R  GG+L++QA + +I  CSL+  P H++D V  W  +L E LS     I+  AI A+P    E+   P  +     K  +I ++Y+ E+    S++ R G +LALG  P+F+L+     ++  L +   ITP ++ WAE RRD++ ALTSI+ T+    G D      ++++Y   L G +DYT D+RGDIGAW+REA++  L ++ L L ++ PE++ E  + +++  +AQQAVEKIDRTR L+ R+F + + +   VP IP + E+   FP   +     W   S TFP F +L+ L  YT  V++G++ SVG LTE LVK +S +LF    +E+    + + +  C  + ++   +QKNDR+T+P  +FLD+L  SG L  ++E+ NS F   +L L++ EIA   D  KL+   +V+C  +Q    +    ALVQLSI LCH+   +R+ T+ KL+E+LL   D S +  E  D V  +LS+T+W++ +E ++P+RN LC LMG+  P   KK
Sbjct:   20 GLGCALEYFSEHEEIFKMIDNLKNLV-AADRSVVEKAYETFSFILSQYIEQPHLVDSHIDDLLQKFIVIVRSNDENME-----LKHLAFKYMFVVVNVRGYKVIVRHLPHEVADFEPVLQLLESQDPNDPETWTTRYILLLWLSIIVMIPFHMSRFDGFDEKETEKKTVMTRILDVIKIYAVVPDKCRDAAAYLSHKFITRYDVKEKHLTSFLDWAMELSLSKDSNVFVKYGTLACIATILKHGKREDLLPHARRLLEWIINAEFKNNVGSNIQKLVYKIVQRIGLTFLPPRVAAWRYKRGNRSLAANLSA----GDGSIPKTSNNDEVEENEDDDIDVPDEVEEVIDQLIQGLRSIDSIVRWTAAKGVGRVTGRLPKDLADEVVGSTLELFSPRESDGAWHGGCLALAELGRRGLLLPERLPQVVPVVLKALVYDEPRGYSSVGSHIRDAACYVCWSFARAYETHILAPHVEDIASTLLVVTCFDKEINCRRAASAAFQENVGRQGSFPHGIDILTAADFFTVSVRNNAYLNVSVYIAQFEEYTLPLIDHLLLRKVDHWDSGIRELSSKALHNLTPKAPEYMIKTVLPALFEKTRSIDLNSRHGATLAIGEVIHALSKFAKEQNTDIKLILKEDLLEKTRNLIPHFRERLYFRGLGGELMKQACSDFIEKCSLSSLPFHNEDIVVDWLKLLNECLSYEVVNIRTAAISALPVLFSEFYCAPDKAQ----KHKEIINQYVSEVLSTTSQIARMGHALALGVLPKFMLQPNLEFVIVSLIESTLITPVSQKWAEGRRDSVKALTSISTTMSVEIGKD-FTKKHILEIYHTFLEGLKDYTQDKRGDIGAWMREASVTGLQTLMLLLTERLPEVLTEDLIVKILCGIAQQAVEKIDRTRALSGRVFYSFIYNNPRVPNIPYYNEITLIFPKE-ECDILNWNSASATFPKFVQLIRLPPYTYSVMLGMICSVGALTETLVKNSSHSLFSYLKEEMKSGGVTEIERMCDVIYKIFVDHQKNDRITVPMFRFLDKLFDSGCLSLVIEDVNSTFIKKILKLIQMEIAGCKDIYKLIDGINVLCQFIQIKGEVC-DIALVQLSILLCHRQSYVRRSTSTKLYESLLVNGDSSNIDPEKLDDVMQVLSNTNWEEPVEEVKPIRNELCGLMGIRVPVPAKK 1173          

BLAST of tubulin-specific chaperone d vs. nr
Match: gi|1069794720|ref|XP_018322258.1| (PREDICTED: tubulin-specific chaperone D [Agrilus planipennis])

HSP 1 Score: 959.133 bits (2478), Expect = 0.000e+0
Identity = 508/1180 (43.05%), Postives = 734/1180 (62.20%), Query Frame = 0

Query:   28 EDPYTNAVGLGCALETFQEAQAVEAWIEGVEAAANQANRLPWEWLYQKFTLALDLYQEQPHLIDPFFPQWIRRLLILIRENPGQPEAGSLGTRHAAAALAAHIIKVRKDKVAVRQFPHEVSDVEPVLALLEAE--HGLERWETVYVLLLWLSMLVLIPFDMGRFD----SGAEVVSLRRRIDAVTRQYLVATVRAQNAAAMLASKFFTRPEIALELLEPFLDWCLSVASDPAQPDVARMGALKALAAVYKQGQRAELLRFTPQLLARIIALELKDHPNALLRQLSLKVIQRCGLIFLPARVAAWRYQRGNRSLVVNLLAE------LAPAAGSEPGGQNMDLAGEDADADYDIPDDIEEVIEELLSGLRDKDTVIRWSAAKGIGRITNRLPRELADDVVESLLGLFSLRESDAAWHGGCLALAELGRRGLLLPERLDAVVRVVLKALVYDEKKGSFSVGSHIRDAACYVCWAFARAYDPDALRPHVDEIACALLTVTIFDREVNCRRAASAAFQENVGRQGTFPFGIDILTQADYYAVGSRNNTYLDLSVFVAQFGDYNVALIDHLIEKKVNHWDLTIRDLTADALHNLSHVNRDLMKSKVLPKLLHLAQGSDLFGKHGAIVAIGTVCLALSKAIEKEGSDLGQYLGPDMVSAIKTIAPALVEKLSLRMTGGDLLRQAITSYISNCSLAKFPIHHDDTVHLWHAILAENLSNSDPLIQEKAIKAVPHFLGEYLKDPKTSTLLVGKRDKIFDEYLHELGN-SELHRRGFSLALGACPEFVLKGREGDILEKLFQCIPITPDTETWAEARRDAILALTSIAQTL--GYGPDGLASSTVVQLYQCLLGGFQDYTLDRRGDIGAWVREAAMGSLCSITLDLMDKSPELVPEICVRELMPSLAQQAVEKIDRTRGLATRLFTTLLLHEQTVPGIPRHQELKSFFPPNLDMTTFPWTVESQTFPIFTRLLNLEEYTERVLVGLVVSVGGLTERLVKYASLALF----QEVDDMSLAQKQHFCRAMLRVLRANQKNDRVTLPFMKFLDQLLTSGHLEDILENENSAFSMDLLGLVKAEIAKSGDPQKLMLSCDVICALLQTHDSLAVKKALVQLSIFLCHKFPRIRKLTANKLFEALLTFSDRSIVPEENFDAVNAILSDTDWDKSIEILRPMRNNLCDLMGVPAPA 1188
            E+P+    GLGCALE F E+  V   I+ +++  +    + +E  Y++F   L  YQEQPHL+D      + + + LIR+    PE      +HA       II VR  K  V++ PHEV+D EPVL +LE +     + W T YVLLLWLS++V+IPF M R D    S     ++ +R+  V + Y+V   + ++AAA L+++F TR +     L+ + +W   ++++P      + G L ++A + K G+R +L+     LL  II+   K+ P   +++L  K IQR GL FLP R+A+WRYQRGNRSL  NL A       L+P++ ++   Q         + + ++PD++EEVI++L+ GL   ++V+RWSAAKGIGR+T RLP++LAD+VV ++L  F+ RE DAAWHGGCLALAELGRRGLLLP RL  V+ V+ KAL YDE +G  SVGSH+RDAACY+CW+FARAYD   L+P V++IA ALL  T FDRE+NCRRAA+AAFQENVGRQGTFP GIDI+T AD+YAV  R+N YL++SV++AQF +Y + LI HLI++KV+HWD+ +R+LTA A HNL+    + M  KVLP L   A   DL  +HGAI+AIG + LALS+  E +G  +   +  +    ++ +     E+L  R  GG+L+RQA ++ I  CSLA  P H+   +  W ++L + LS    +I+  A+ A+  F  EY K    S  L+ K++ I   Y+ E+ + S+++R G+SLALG  P+F+LK     IL  L + + I   T+ W+E+RRD I A+ SI  TL    G + ++S+ ++ +Y+    G  +YT D+RGDIGAWVREA+M  L ++TL L  K P L+    V +++  LAQQAVEKIDRTR LA ++F+ ++  +  VP IP H  L   F    +     W   + TFP F +L+ LEE+T  +++G++ SVGG+TE LVK +S ALF    ++     L + Q  C  +  +    QK DR+T+  ++FL +++TSG L +IL+ + S F+  +L LV+ EIA   D  KL+   DV+C  +Q    +    ALVQLSI LCH+   IR+ TA KL+EALL + D S++P EN D +  ILS T+W++ +E LR +RNNLC LM +  P 
Sbjct:   15 EEPF----GLGCALELFSESVEVMNMIDSIKSVVSAPISV-FERAYERFVYILTQYQEQPHLLDSHLEVLLEKCIALIRDQSNPPE-----LKHAVFKYMHVIISVRGYKDVVKRLPHEVADFEPVLQMLEQQDPSDSDTWFTRYVLLLWLSIIVMIPFHMSRLDGFTNSNDNKKTVMQRVFEVCKVYIVVPDKCRDAAAYLSARFLTRSDTKEHYLKSYFNWTYEISTNPESSVFVKSGCLASIAMILKYGKREDLVAHVGNLLQWIISANFKNDPGTNIQKLVYKNIQRIGLTFLPVRIASWRYQRGNRSLASNLSAGDGEKDFLSPSSPTDSNEQ---------EENIEVPDEVEEVIDQLIHGLACNNSVVRWSAAKGIGRVTGRLPKDLADEVVGTILESFNPREGDAAWHGGCLALAELGRRGLLLPVRLPEVIPVIQKALFYDEPRGYSSVGSHVRDAACYLCWSFARAYDTSVLKPFVNQIASALLVATCFDREINCRRAAAAAFQENVGRQGTFPHGIDIVTVADFYAVSIRSNAYLNISVYIAQFNEYTMTLITHLIDRKVDHWDIAVRELTAKAFHNLTPSAPEYMAKKVLPSLFEKATSIDLNSRHGAILAIGEILLALSRLSEDKGVKMEDLVTVETFKQVECLIRNFQERLYFRGMGGELMRQACSNLIEKCSLAHMPFHNTKVIEDWLSLLNDCLSYEVQIIRTCAVHALLPFFSEYFKG--DSVDLLAKKEMIVTRYVKEMKSPSQVNRMGYSLALGMLPDFMLKPHLNIILAGLIEAMCIDQHTQKWSESRRDIIKAINSICCTLEDELGKE-ISSNDIMAIYEVYFTGLSEYTQDKRGDIGAWVREASMSGLQTMTLLLAKKCPHLLTSQVVTKVVAGLAQQAVEKIDRTRALAGKMFSNIIHSDPRVPNIPHHDVLVGIFSQE-ECDALNWHSANSTFPKFVQLIELEEFTYNLMLGIITSVGGMTETLVKNSSSALFTYLKEQEKKKGLEEIQRVCETISEIFHNYQKVDRITVSILRFLSRIMTSGCLTNILDFQKSDFAKKILKLVQLEIAGCRDVYKLVDGIDVLCQFIQVRSEVC-STALVQLSILLCHRQIYIRRSTATKLYEALLVYGDNSVIPSENLDEIMNILSMTNWEEPLENLREIRNNLCTLMNIKKPV 1170