BLAST of PREDICTED: nesprin-1-like vs. L. salmonis genes
Match: EMLSAG00000001706 (supercontig:LSalAtl2s:LSalAtl2s1294:57710:172286:-1 gene:EMLSAG00000001706 transcript:EMLSAT00000001706 description:"maker-LSalAtl2s1294-snap-gene-0.48")

HSP 1 Score: 221.09 bits (562), Expect = 2.311e-57
Identity = 123/304 (40.46%), Postives = 172/304 (56.58%), Query Frame = 0

Query:  617 YDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSV-----------PEKW---------------KLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPE 894
            Y+ ++ + +DE++ +QKKTF  W+N +L K      + DL + LRDG  LL+LLEVL GE LP E+GR+  R H + NV TAL +LR K  KLVNI   D+VDG P + LGLIWTIIL+FQ  ++      +  R  S +   S      +       GG R ++            P +W                + A++ALL W Q    +  GV V+DF +SWRDG AF AII+  +P  VD   + +   + R+++AF++ E + GVTRLLDPEDVD P PDEKS++TYV+Q    +PE
Sbjct:   58 YEENLTKFKDERDAIQKKTFTKWVNKHLKK--ANRNVRDLFEDLRDGHNLLSLLEVLAGEILPRERGRM--RFHAIQNVETALRFLRYKEXKLVNIRGEDIVDGNPKLTLGLIWTIILHFQNHQHHYYRHIMPIRSSSLSKKSSSKFMCCTS---AAAGGSRTVTYRENQSKFLWLDPSRWVEKKKEISDIVVGQEDVSAKEALLRWSQKTTQRYPGVKVSDFTQSWRDGLAFNAIIHRNRPDLVDWKKLEKRQVRERIDSAFHIMEREYGVTRLLDPEDVDTPEPDEKSIITYVSQLYDVFPE 354          

BLAST of PREDICTED: nesprin-1-like vs. L. salmonis genes
Match: EMLSAG00000006584 (supercontig:LSalAtl2s:LSalAtl2s361:367157:370951:1 gene:EMLSAG00000006584 transcript:EMLSAT00000006584 description:"maker-LSalAtl2s361-augustus-gene-2.17")

HSP 1 Score: 208.379 bits (529), Expect = 2.844e-56
Identity = 139/590 (23.56%), Postives = 293/590 (49.66%), Query Frame = 0

Query: 1727 YEKKFSQMVRWMDTVDNSLAKMFKGQA-DANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWH--EYKENKVHMLRLIDDSEFELNKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRD---LLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITK----LDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLF----ENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKAL 2302
            Y+ ++++MV WMD V+ ++ K+   +   ++ F+ EK  F  LC++++ R +  +WL + LD+L+ +R  +    E ++LE ++ R+K +IP I+ T+ K + Y K Y +  +   +   ++D++++SI++ F A +E  +  + +++ +L  +++ R  V+  + KG  LM DP  P FL   V+ L+  W D   +   R+K  T+  + W   E ++ + H    + D+E+ L K +          Q R++        +  E+I   +    D +     P+ + A   EMN   Q+++    +L+  D+K++++ + + +    + N  E++ W+   + +++ I K      +SP+D+V  T+E+ +D++ K +   K E +  ++F    E VP  A        ++ ++K ++ +      +    ++D+  W      +   +PW+++AE++   GL + ++L +A   L   K F+EE   K K L  AA     ++        V++L+     +     +W+ R+  LV+ W   D    E+  W+    ++    +   ++SI+ L G+L+QLK +
Sbjct:   15 YDTRYAKMVEWMDNVEMAIQKVCASKVYTSSEFKREKDNFHSLCKNLE-RAETKRWLAETLDTLMKERVGEEKKEELRKLEVIMERHKMLIPRIQETLVKTECYWKCYSYGDDLIPIFEFIDDLRHRSIKEVFSANQEHTEEHIEKQDKVLNSVENKRRMVIDFISKGDRLMTDPNCPKFLYGHVQKLKEAWEDTVEKAQARKKALTDNFQSWETFEIQQGECHKQLDLADTEYNLIKKIFDLKQGPADYQNRLQTAAV--YRQTIEEIFSTISTCNDIIQLLL-PDDKKA---EMNGKVQEIQTRMAVLQNTDKKLEYINDFNKRLAVLNTNATEIESWLQDGRKRIDDIIKPADSSTLSPEDKVTKTMEIMEDLQKKSEFTRKQEIEKEDIFPKGDERVPSDA--KKFIARLDKIRKTIDNMEEESKTECAQFSQDIKYWAEFTTGIKEFEPWIKRAEIRKNEGLIKPSSLMEACQILGDSKNFQEECEQKIKTLDDAAASAQKMTTHIEADTKVNSLRELWLVIHEASKEWVSRMTTLVECWNKLDGNVGELSSWVST--SDSTAPEGESEISIEKLEGQLNQLKIM 593          

BLAST of PREDICTED: nesprin-1-like vs. L. salmonis genes
Match: EMLSAG00000001168 (supercontig:LSalAtl2s:LSalAtl2s1194:133284:146626:-1 gene:EMLSAG00000001168 transcript:EMLSAT00000001168 description:"augustus_masked-LSalAtl2s1194-processed-gene-1.6")

HSP 1 Score: 196.438 bits (498), Expect = 7.427e-50
Identity = 116/298 (38.93%), Postives = 162/298 (54.36%), Query Frame = 0

Query:  621 IHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERL-PTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYP---EGAVAKGSDRFGSLDSQYQEL 914
            I  +++E+  +QKKTF  W+NS+L K    ++IEDL   L DG  LL LLE++ GE+L     G++  R H + NVN  L +L +K ++L  I A D+VDG P ++LGLIWTIIL FQ +E                          + DD+   G           K  A+ ALL WCQ +      V + DF  SWRDG  F A+I+S +P  VD   +    +   LE AF VAE   G+++LLDPEDVD+  PDEKSV+TYVA F H +    EG   +G  R G++ S+ +++
Sbjct:   29 IRALQEERLHIQKKTFTKWVNSFLQK--ARLEIEDLFTDLSDGRNLLKLLEIISGEKLGKPNHGKM--RVHKIENVNRCLAFLHTK-VRLXXIGAEDIVDGXPRLILGLIWTIILRFQXQE-----------------------IEINVDDQEENGE----------KKSAKDALLLWCQRRTQDYHNVNIRDFTSSWRDGLGFNALIHSQRPELVDYNRLDPADHIGNLEXAFEVAERNFGISKLLDPEDVDIAKPDEKSVLTYVASFYHTFSRLKEG--HRGGKRIGNIVSKIRDV 286          

BLAST of PREDICTED: nesprin-1-like vs. L. salmonis genes
Match: EMLSAG00000006068 (supercontig:LSalAtl2s:LSalAtl2s329:20859:102914:-1 gene:EMLSAG00000006068 transcript:EMLSAT00000006068 description:"maker-LSalAtl2s329-augustus-gene-1.14")

HSP 1 Score: 184.882 bits (468), Expect = 2.296e-46
Identity = 99/321 (30.84%), Postives = 189/321 (58.88%), Query Frame = 0

Query: 2907 LATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKCLDRDKKEVMDEIAKLKIDISELLLSWRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNKGAQMDAINEYE 3227
            L  LR+KA+ C+D +G D ++  RL+ I ++    ++F++ +  I  +IK+L  +++ ++V  L K+L +L K+YD +SSQ++K    L+ +LEK Y +KVQ  +K I+  KEK+ WC PD   DKF+L +K++ L+E+   L+   D +  L  L+ +LL++ D+DK  E+ +T+ CLD    E+ +E+  +   ++  +  +    L  ++ ++W+K +E   +  +    +LE+ K+IS   EQ++  + + +  + EL + +  ++   P +R     + +K++YE FK ++  QL  ++DL  N+ AQ + I+ YE
Sbjct:    8 LINLRDKALKCDDLSGTDEDLYNRLISIREINTGLENFDDRLIPITQDIKNLHERYEGSEVNNLMKDLGNLEKKYDALSSQVSKIMSTLFGLLEKRYSDKVQDLLKCISLNKEKVAWCSPDPDSDKFALNSKLETLSEIRDALKQLIDSNEDLKILSKILLKLVDEDKVKEINETIYCLDNGIAEIQNELISVNDLLNSAIALFNKYALKMEEASNWIKETEESIKTNLIA--DLESAKQISGDCEQIENNILSYEPTVTELVELSKEILRKHPASRAGGTADHIKSKYEHFKATISEQLSNIKDLIKNQSAQKNYIDAYE 326          

BLAST of PREDICTED: nesprin-1-like vs. L. salmonis genes
Match: EMLSAG00000012718 (supercontig:LSalAtl2s:LSalAtl2s965:459122:472828:1 gene:EMLSAG00000012718 transcript:EMLSAT00000012718 description:"maker-LSalAtl2s965-augustus-gene-3.36")

HSP 1 Score: 177.563 bits (449), Expect = 3.317e-44
Identity = 112/275 (40.73%), Postives = 149/275 (54.18%), Query Frame = 0

Query:  619 SHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPT-EKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKY 892
            S I  +  E+E VQKKTF  W+NS+L +     K+ DL   LRDG  L+ LLE+L GERLP   KGR+  R H L NV+ AL +L  +R+ L N+ A D+VDG P + LGLIWTIIL FQI+E T  +E  G                        PG   +          A+ ALL WCQ +      V + +F  SW DG AF AII+  +P  +    + + +    L  AF VAE +  +T+LLDPEDV+V  PDEKS++TYV  + H +
Sbjct:   40 SRIKALAVEREHVQKKTFTKWVNSHLCR--VNYKVNDLYVDLRDGKLLIKLLEILSGERLPRPTKGRM--RIHCLENVDKALSFLYEQRVHLENMGAHDIVDGSPRLTLGLIWTIILRFQIQEIT--VEEPG------------------------PGQATR---------SAKDALLLWCQMKTAGYNNVNIRNFTTSWSDGLAFNAIIHKHRPDLIQYDRLAKSNAHHNLSNAFDVAEREFNLTKLLDPEDVNVERPDEKSLITYVVTYYHYF 275          

BLAST of PREDICTED: nesprin-1-like vs. L. salmonis genes
Match: EMLSAG00000006540 (supercontig:LSalAtl2s:LSalAtl2s358:369162:372163:1 gene:EMLSAG00000006540 transcript:EMLSAT00000006540 description:"maker-LSalAtl2s358-augustus-gene-2.24")

HSP 1 Score: 152.91 bits (385), Expect = 4.339e-39
Identity = 117/452 (25.88%), Postives = 221/452 (48.89%), Query Frame = 0

Query: 1727 YEKKFSQMVRWMDTVDNSLAKMFKGQADANT-FEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLD--VSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLF---ENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAE 2172
            Y+ + ++M  WM+ V+ +++++   +   +  F+ E+  F  LC+D++ R +  KWL Q L+ L+++RA +    +  +L+ LI +++ +IP +  T  KVD+Y K Y +  E       L+ I   S  D  P+  E+V  L+ R+E  L QL+  R  V  L+ KG  L+ +P  P FL   VK +E  W+D   +   R +   E    W  Y    V +    + +E E+ K   + + +     L  ++ +  +     E I   +++  D ++     E++  +  E+ A+  KL  ++ + +EK++ +         F K+L  L  W+  A+ +LN I      ++P+DRV +T+ELQ+DV  K++ + +   +   L    + VP  A   D K E++ +++ +  L   V  +    ++D+  W   +  +   +PWLE AE
Sbjct:   15 YDMRQTKMAEWMENVEKAISRIMADKVYTSAEFKRERDNFHALCKDLE-RVEVKKWLQQILEILMAERAKEERKEQLGKLDALIKKHEELIPTVLKTQVKVDLYWKCYAYGDELKPHIEFLDGIMLSSTRDIAPSCVENVDELIERQEKSLTQLETKRNVVKELIGKGKQLLENPDKPKFLXSHVKRIEEGWDDTKEKASARLQLLQETKAAWEGYAXGLVQIGDEFEKAEDEIKKVKKRFNLQSAFDDLEKRQKIFADTKNTVETIYKSIQDNYDIMTMTLPDEKKDFVKKEVKAVTDKL-GVVNKFEEKVKKIETFVNSLNGFDKSLKTLNTWMTDAETQLNDIKNNSDKMTPEDRVSLTMELQEDVAAKVEIIRENIKNEEELLPQGDKVPQDA--QDFKDELKRIEEFIVNLQKKVMQECDNFSEDVKYWAEYKTGIKEFRPWLENAE 462          

BLAST of PREDICTED: nesprin-1-like vs. L. salmonis genes
Match: EMLSAG00000005364 (supercontig:LSalAtl2s:LSalAtl2s289:366226:374953:1 gene:EMLSAG00000005364 transcript:EMLSAT00000005364 description:"maker-LSalAtl2s289-augustus-gene-3.16")

HSP 1 Score: 156.377 bits (394), Expect = 2.834e-38
Identity = 93/266 (34.96%), Postives = 146/266 (54.89%), Query Frame = 0

Query:  629 ERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPT-EKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPS-PDEKSVMTYVAQFLHKY 892
            E+ Q+KTF  W+NS+L K     +IE + +  R+G +L+ LLEV+ GE LP  ++GR+  R H ++NVN AL+++ SK +KLV+I A ++VDG   + LG+IWTIIL F I++                                       +SV E   + A++ LL WCQ +      V V +F  SWRDG AF A+I+  +P  +D + + +++    L  AF +AE  L + ++LD ED+   + PDE++VMT V+ + H +
Sbjct:   39 EKQQRKTFTAWVNSHLRK--AGTQIESIEEDFRNGLKLMLLLEVISGETLPKPDRGRM--RFHMIANVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQD---------------------------------------ISVEE---MTAKEGLLLWCQRKTAPYKNVNVQNFHLSWRDGLAFCALIHRHRPDLLDYSKLSKDNPLENLNLAFDIAERYLEIPKMLDAEDLSSTAMPDERAVMTCVSSYYHTF 258          

BLAST of PREDICTED: nesprin-1-like vs. L. salmonis genes
Match: EMLSAG00000008674 (supercontig:LSalAtl2s:LSalAtl2s537:351087:359440:1 gene:EMLSAG00000008674 transcript:EMLSAT00000008674 description:"maker-LSalAtl2s537-snap-gene-3.38")

HSP 1 Score: 143.665 bits (361), Expect = 5.167e-35
Identity = 95/270 (35.19%), Postives = 130/270 (48.15%), Query Frame = 0

Query:  629 ERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQT-RLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAV 897
            E+VQKKTF  WIN+ LA R   + I DL   LRDG  LL LL VL       EKG++  R H+L N  T L  L+  ++KLVNIN  D+VDG P + L L+W+II+++Q  +                V +     G S                     G  K LL W  +   + +   V DFG SWR+G AF A++   +P  V   +  +  N T RL+  F      LG+ +LLDPED  + +PD+KS+M Y+       P   V
Sbjct:    9 EKVQKKTFGKWINAQLAGR--GIVITDLYYDLRDGHVLLELLGVLTNRSFVKEKGKL--RVHHLGNAYTILSILKENKVKLVNINNVDLVDGNPKITLALVWSIIVHWQFRD----------------VMKESIVKGSS---------------------GLEKILLAWTCSATQKHYPDSVKDFGDSWRNGLAFGALLAHFRPDLVVWDNYLRIQNHTDRLDFIFNTIRDHLGIQKLLDPEDF-LSTPDKKSLMMYIMCLYDVLPHDIV 236          

BLAST of PREDICTED: nesprin-1-like vs. L. salmonis genes
Match: EMLSAG00000006304 (supercontig:LSalAtl2s:LSalAtl2s343:279672:293390:1 gene:EMLSAG00000006304 transcript:EMLSAT00000006304 description:"maker-LSalAtl2s343-augustus-gene-3.20")

HSP 1 Score: 113.62 bits (283), Expect = 8.994e-25
Identity = 81/263 (30.80%), Postives = 131/263 (49.81%), Query Frame = 0

Query:  627 EQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPG-AVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQF 888
            E +R+Q+ TF  W N +L  R     I DL   L DG +L+AL+EVL G+++P    +   R   L NV+ AL++L  + I LVNI++ D+VD +  +++GLIWT+I+++ I        S+    G P                   GG      P       ++ L+ W + +      + + +F   WRDG A  A+++S+ PG   D  +   +        A  +A+  L V +LL PE++  P+ DE S+MTY++QF
Sbjct:   66 EWKRIQQNTFTRWANEHL--RQANRSISDLETDLSDGLKLIALIEVLSGKKMPRHNKKPNFRSQKLENVSIALQFLEMEGITLVNIDSTDIVDCKLKLIMGLIWTLIIHYAI--------SMPVWDGPPL------------------GGSASQRTP-------KQRLMDWVKDKCPD---LPIQNFTDDWRDGKAVGALVDSVAPGLCPDWANWDPKKPVENAREAMDLADEWLNVPKLLTPEEIVNPNVDEPSMMTYLSQF 290          

BLAST of PREDICTED: nesprin-1-like vs. L. salmonis genes
Match: EMLSAG00000010063 (supercontig:LSalAtl2s:LSalAtl2s663:144957:208544:-1 gene:EMLSAG00000010063 transcript:EMLSAT00000010063 description:"maker-LSalAtl2s663-augustus-gene-2.10")

HSP 1 Score: 81.6481 bits (200), Expect = 1.553e-15
Identity = 78/316 (24.68%), Postives = 146/316 (46.20%), Query Frame = 0

Query: 2028 DLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLD--VSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKD-----VEALNMNVDAKSVAIAKDL----DIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTA 2332
            ++L   DEK++ L E +     F   L EL++W+ + K + +++      + P +RV  T+ELQ DV     Q+EK +  A    +  P  A  +  + +    ++D     +  +N  V A+     +D+    D    C+     + PW++KAE K AMG+PR  NL +A +   Q K +  +    + +  Q+ +    ++  +       ALK RL AV     +WI + + ++K W    +   E    + A +++  G  +  ++ ++ L   LD LK +     +KQ  + GLS++    A+  S++ A
Sbjct:  200 EVLNRMDEKLKFLSEFNSTIKVFDGTLTELENWLIEGKRRKDELLNPTETIEPQERVMATMELQSDVDT---QIEKTKAAAEEWDKLKPTEAGEDTPEAKSFASRQDAMSSTLSTMNDEVRAEGAKFGEDVKYLADFTAGCKR----VDPWVKKAEAKKAMGMPRPNNLVEAKDFFNQTKIWLADAXXXDNIXEQSXESAKKMTLHEDSDVKYKALKERLAAVLVIAKEWIEKYDGMIKVW----DKQAETAAKVSAAISSKPGDGSGSEMKLEDLEKHLDSLKLM---FIEKQKMMEGLSQEAANAAILESKEEA 501          

HSP 2 Score: 70.4774 bits (171), Expect = 4.845e-12
Identity = 53/181 (29.28%), Postives = 91/181 (50.28%), Query Frame = 0

Query: 1727 YEKKFSQMVRWMDTVDNSLAKMFKGQADANT-FEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDI---QNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAP 1903
            Y+ +  +MV  MD  D S+A + K     +  F+  K+ F    R+++ + +  +WL    ++L++ ++      E K+L+ L+ RY ++IP IE T   VD   KSY F  +   +   L D+    ++SI  N  +  ED    + ++E  L QLD  R  V+    KG  ++ DPK+P
Sbjct:   15 YDARAEKMVDLMDKTDKSIANVVKDVIYTSAEFKKVKENFVSCARNLE-QPETAEWLSSTKETLMTDKSGKEQDEEAKKLKFLLERYDTLIPKIEDTKNVVDCLWKSYQFTDDLAPLMEWLEDMVSRSSRSINTNSASQTED---HIEKQEKTLDQLDKKRKVVMENQTKGEKILSDPKSP 191          

BLAST of PREDICTED: nesprin-1-like vs. SwissProt
Match: gi|425906075|sp|Q8NF91.4|SYNE1_HUMAN (RecName: Full=Nesprin-1; AltName: Full=Enaptin; AltName: Full=KASH domain-containing protein 1; Short=KASH1; AltName: Full=Myocyte nuclear envelope protein 1; Short=Myne-1; AltName: Full=Nuclear envelope spectrin repeat protein 1; AltName: Full=Synaptic nuclear envelope protein 1; Short=Syne-1)

HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0
Identity = 609/2509 (24.27%), Postives = 1181/2509 (47.07%), Query Frame = 0

Query:  621 IHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPE--GAVAKGSDR------FGSLDSQYQEL----RAWLTTKISWFEQM-------QVTKGQLPMNYDEFQ--KCKAEMKNKQEIYERLRQLKSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDDIPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQF-ETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEY-KKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAV-GEPEDLQIELAKHKPFLAKTVNMQ---SLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGE-KQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQ--LDDHRGPVLSLLQKGLDLMR--------DPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELA-DALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLDVSPD---DRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGL---PRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANL-SCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSID--ALTGELDQLKAL-NDNLTQKQV--ELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPF--------NHIDSSLENVHSLAQEHADNQSRIAKVEINAKNK---ILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLEQTLDNVTSLDGLEIALAE--LDSLAQQCQSKKNDGANDEEMAAKSKTYVIQNGKP---TSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVA----ELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQMVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLET---AKGKLSKYDNDLPSMMSQKANVAAKMALLVE--SNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLAT----LREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQS----EIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNEL 3045
            + +++DEQE VQK+TF  WINS+LAKR PPM ++DL + ++DG +LLALLEVL G++LP E+GR ++R H ++N+ TAL++L  ++IKLVNIN+ D+ DGRP++VLGL+WTIILYFQIEE T  L  L Q   S A       + E      TP    K  V  K +  A+KALL W Q   G++ G+ V DFGKSWR G AF ++I++I+P  VD+ +++  SN+  LE AF +AE +LG+ RLLDPEDVDV  PDEKS+MTYVAQFL  YP+   A   G +       F S  +  Q      R        W EQ        Q+ +  L   Y  F+  + + EMK KQ I   ++ L  + +L +       +  +W +V +++  W   LD +LP   G IG WL + E  +  +     ++EE A  + +K+E HK    +  A +  F E   T S     +P DQL+ MA+R   +   +++  ++++FLE K  +++ L+L ESKLK+W IKYGR E V+Q++  Y +F+  +K F++++  +   +  +  Y K DG +  EE++++ +F++E   +W+N S E+R V+S+LEEV++ W R+      LQ ++ DA + L ++E+A+++FF +  +W +++  + D   FL+ T D  V +DL+++ ++++  W +LF + + +  + ++ R K+ Y + +  L  +  +A   L+   +V   ++K  ++ L ++   V  M+  +K +++    +  E   ++ + M  ++ K KE L  ++      +     LL  +E     E+++  + D +  + + +  +  E +   +   KH+  LA   N +   +L++      Q  +  +   +  D + + R++  I+  F+  ++    ++ ++ +  +L  KF +++  +   L+ A+     K  +  +++++H   F+   + +LN +L    +L   L E ++  ++E    +  +W ++   AP+H L  +  + +++   S++E   +L  E++     E   +II+EH  +F+         + L  + E+  +     P    + D   T       +   I +  ++L + P++W++Y  +FS+   W+ T +  L K  KG+A      GE K+  +E+   V  R + + WL  +L  L    +++    +   L  L + +K+++ ++    + +  +     ++     V N L ++ + S E      +E  + +++ E     Q  L  H+     +  K  D+ +        +   P    EE++ LES  +        + +     L+ W  ++ NK  ++R +    F+   +  +         L  +    +E  K TE I  +   L  +A      P+ +  L  +  +++++++ L +  +E I+ +  +  KW  F  N   L  W+ + + +LN +     + D    ++K+T+   Q+++ K+  +  LE +A + F     T     +K ++  +++  E L  +       I   L      + +L  I+  + + E K+A+ +      T     L E   L +  E +         +A K  N  SC Q    +  AL+ + + +     +    LE+L+  W+  +        W+E      K      D+S D   + GEL  ++AL N+ ++Q     +LL L +    VA   S+D  +++K  +  L  +  DL      +++ +   +   Q+F + +     W+K   S +   +E+           H D + E V S   E    Q  +AK+    + +   +L        + + +   ++E ++ +L   + F   HA     L  L QT++   S++  E  L E  L+   Q  ++ ++   + + + +K++ Y ++ G     TS       +   + ++++++  K+ +  +L      F+D +++L   ++ + ++  K  ++E T   L+           EL++   +   LKD  +++ ++ V   P+    V   ++     WD    L+ E QG+   LI L  +    K  V   L+     +  +  T I+ ++D+     K     + +   +   + F  K   L+ EL+ + + D + ++TDM+    K+  + + L+ N++ L   + +W       D++ GW   + +     A NI++L+    A+  L ++++++ +   +   + +K+  L E   N   P + LQ    D+ Q   + +     + E  +G  K   A   +++   +D+ T    + E  M C       +E  E L  +  +Q E    + ++ + +  +  L  K+ +A++  L + ++ L K+++ VS   +KT  +L   LEKH+ E +Q      + + A+AKE       D+ GD   LE K+  L  +
Sbjct:   18 MQRLQDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGVKLLALLEVLSGQKLPCEQGRRMKRIHAVANIGTALKFLEGRKIKLVNINSTDIADGRPSIVLGLMWTIILYFQIEELTSNLPQL-QSLSSSASSVDSIVSSE------TPSPPSKRKVTTKIQGNAKKALLKWVQYTAGKQTGIEVKDFGKSWRSGVAFHSVIHAIRPELVDLETVKGRSNRENLEDAFTIAETELGIPRLLDPEDVDVDKPDEKSIMTYVAQFLKHYPDIHNASTDGQEDDEILPGFPSFANSVQNFKREDRVIFKEMKVWIEQFERDLTRAQMVESNLQDKYQSFKHFRVQYEMKRKQ-IEHLIQPLHRDGKLSLDQAL---VKQSWDRVTSRLFDWHIQLDKSLPAPLGTIGAWLYRAEVALREEITVQQVHEETANTIQRKLEQHKDLLQNTDAHKRAFHEIYRTRSVNGIPVPPDQLEDMAERFHFVSSTSELHLMKMEFLELKYRLLSLLVLAESKLKSWIIKYGRRESVEQLLQNYVSFIENSKFFEQYEVTYQILKQTAEMYVKADGSV--EEAENVMKFMNETTAQWRNLSVEVRSVRSMLEEVISNWDRYGNTVASLQAWLEDAEKMLNQSENAKKDFFRNLPHWIQQHTAMNDAGNFLIETCDEMVSRDLKQQLLLLNGRWRELFMEVKQYAQADEMDRMKKEYTDCVVTLSAFATEAHKKLSEPLEVSFMNVKLLIQDLEDIEQRVPVMDAQYKIITKTAHLITKESPQEEGKEMFATMSKLKEQLTKVKECYSPLLYESQQLLIPLEEL---EKQMTSFYDSLGKINEIITVLEREAQSSALFKQKHQELLACQENCKKTLTLIEKGSQSVQKFVTLSNVLKHFDQTRLQRQIADIHVAFQSMVKKTGDWKKHVETNSRLMKKFEESRAELEKVLRIAQEGLEEKG-DPEELLRRHTEFFSQLDQRVLNAFLKACDELTDILPEQEQQGLQEAVRKLHKQWKDLQGEAPYHLLHLKIDVEKNRFLASVEECRTELDRETK-LMPQEGSEKIIKEHRVFFSDKGPHHLCEKRLQLIEELCVKLPVRDP----VRDTPGTCHVTLKELRAAIDSTYRKLMEDPDKWKDYTSRFSEFSSWISTNETQL-KGIKGEAIDTANHGEVKRAVEEIRNGVTKRGETLSWLKSRLKVLTEVSSENEAQKQGDELAKLSSSFKALVTLLSEVEKMLSNFGDCVQYK---EIVKNSLEELISGSKE-----VQEQAEKILDTENLFEAQQLLLHHQQKTKRISAKKRDVQQQIAQAQQGEGGLPDRGHEELRKLESTLDGLERSRERQERRIQVTLRKWERFETNKETVVRYL----FQTGSSHERFLSFSSLESLSSELEQTKEFSKRTESIAVQAENLVKEASEIPLGPQNKQLLQQQAKSIKEQVKKLEDTLEEDIKTMEMVKTKWDHFGSNFETLSVWITEKEKELNALETSSSAMDMQISQIKVTI---QEIESKLSSIVGLEEEAQS-FAQFVTTGESARIKAKLTQIRRYGEELREHAQCLEGTILGHLSQQQKFEENLRKIQQSVSEFEDKLAVPIKICSSATETYKVLQEHMDLCQALESLSSAITAFSASARKVVNRDSCVQ----EAAALQQQYEDILRRAKERQTALENLLAHWQRLEKELSSFLTWLER--GEAKASSPEMDISADRVKVEGELQLIQALQNEVVSQASFYSKLLQLKESLFSVA---SKDDVKMMKLHLEQLDERWRDLPQIINKRINFLQSVVAEHQQFDELLLSFSVWIKLFLSELQTTSEISIMDHQVALTRHKDHAAE-VESKKGELQSLQGHLAKLGSLGRAEDLHLLQGKAEDCFQLFEEASQVVERRQLALSHLAEFLQSHASLSGILRQLRQTVEATNSMNKNESDLIEKDLNDALQNAKALESAAVSLDGILSKAQ-YHLKIGSSEQRTSCRATADQLCGEVERIQNLLGTKQSEADALAVLKKAFQDQKEELLKSIEDIEERTDKERLKEPTRQALQQRLRVFNQLEDELNSHEHELCWLKDKAKQIAQKDVAFAPE----VDREINRLEVTWDDTKRLIHENQGQCCGLIDLMREYQNLKSAVSKVLENASSVI--VTRTTIKDQEDLKWAFSKHETAKNKMNYKQKDLDNFTSKGKHLLSELKKIHSSDFSLVKTDMESTVDKWLDVSEKLEENMDRLRVSLSIWDDVLSTRDEIEGW---SNNCVPQMAENISNLDNHLRAEELLKEFESEVKNKALRLEELHSKVNDLKELTKNLETPPD-LQFIEADLMQKLEHAK----EITEVAKGTLKDFTAQSTQVEKFINDITTWFTKVEESLMNC-----AQNETCEALKKVKDIQKELQSQQSNISSTQENLNSLCRKYHSAELESLGRAMTGLIKKHEAVSQLCSKTQASLQESLEKHFSESMQEFQEWFLGAKAAAKES-----SDRTGDSKVLEAKLHDLQNI 2452          

BLAST of PREDICTED: nesprin-1-like vs. SwissProt
Match: gi|292630942|sp|Q6ZWR6.2|SYNE1_MOUSE (RecName: Full=Nesprin-1; AltName: Full=Enaptin; AltName: Full=KASH domain-containing protein 1; Short=KASH1; AltName: Full=Myocyte nuclear envelope protein 1; Short=Myne-1; AltName: Full=Nuclear envelope spectrin repeat protein 1; AltName: Full=Synaptic nuclear envelope protein 1; Short=Syne-1)

HSP 1 Score: 595.504 bits (1534), Expect = 2.531e-169
Identity = 580/2477 (23.42%), Postives = 1141/2477 (46.06%), Query Frame = 0

Query:  621 IHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPE--GAVAKG----------SDRFGSLDSQYQELRAWLTTKISWFEQM-------QVTKGQLPMNYDEFQ--KCKAEMKNKQEIYERLRQLKSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDDIPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQF-ETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGEREL-LQWCDDVDSL--VKTVQAVGEPEDLQIEL--AKHKPFLAKTVNMQ---SLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEE---------DVQALVNREEAILQQLDDHRGPVLSL----LQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELA-DALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLDVSPD---DRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQD---ALNELTQLKRFEEEIFGKEKLLVQAADKGANL-SCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSID--ALTGELDQLKAL-NDNLTQKQV--ELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKV--EINAKNKILS--------NSIRAMQKKYLDLGDL-------LENKKKSLQRWSSFCTWHAESDSALNHLEQTLDNVTSL-----DGLEIALAELDSLAQQCQSKKNDGANDEEMAAKSKTYVIQNGKP---TSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQ----KINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQMVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLET---AKGKLSKYDNDLPSMMSQKANVAAKMALLVE--SNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDD----LATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHY 3003
            + +++DEQE VQK+TF  WINS+LAKR PPM ++DL + ++DG +LLALLEVL G++LP E+G  ++R H ++N+ TAL++L  ++IKLVNINA D+ DGRP++VLGL+WTIILYFQIEE T  L  L     S +   S   T     +  +P   RK  V  K +  A+K LL W Q   G++ G+ V DFGKSWR G AF ++I++IQP  VD+  ++  SN+  LE AF +AE +LG+ RLLDPEDVDV  PDEKS+MTYVAQFL +YP+  GA   G          ++    L    ++ R  L     W EQ        Q+T+  L   Y  F+  + + EMK KQ +   ++ L+ + +L +       +   W++V +++  W   LD +LP   G IG WL + E  +  +     ++EE A  + +K+E HK    +  A +  F E   T S     +P DQL+ MA+R   +   +++  ++++FLE K  +++ L+L ESKLK+W IKYGR E V+ ++  Y +F+  +K F++++  +   +  +  Y K  E   EE++++ +F+ E   +W+N S E+R V+S+LEEV++ W R+      LQ ++ DA + L ++E A+++FF +  +W +++  + D   FL+ T D  V +DL+++ ++++  W +LF + + +  + ++ R K+ Y +    L  +  +A   L+   +V   ++K  ++ L +L   V  M+  +K +++K      E   ++   M+ ++ K KE L  ++            LL + +   +   EL  Q     DSL  +  + +V E E     L   KH+  LA   N +   +L++      Q ++ +++ ++  D + + +++  ++  F+  ++    ++ ++ +  +L  KF +++  +   L+ A+     K  +  +++++H   F+   + +LN +L    +L   L E ++  ++E    +  +W ++   AP+H L  +  + +D+ + +++E   +L+ E++     E   +II+EH  +F+         + L  + E+  +     P    + D           +   I      L   P++W++Y  +FS+   W+      L K+     D    +  K    E+  D+  + + + WL  +L  L+   +++        L  L + +K+++ ++    + +  + +   ++           +I   S+E      +E         D + L+  ++ +L      +  ++S     LQ+ ++  +        +EE++ LES           + +     L+ W  ++ NK  ++R +    F+   +  +         L  +    +E  K TE I  +   L  +A      P  +  L  +  ++++++  L +  +E I+ +  +  KW  F  N   L  W+ + + +L+ +     + D    ++K+T+   Q+++ K+  +  LE +A + F     T     +K ++  +++  E L  +       I   L      + +L  I+  + +   ++A  +   ++  +    L E   L +  E +     +   +A K  N  SC Q    +  AL+ + + +     +    LEDL+  W+  + G      W+E         +   D+S D   +  EL  ++AL N+ ++Q  +   LL L +    VA   S++   V+K ++  L  +  DL      +M  +   +   ++F + +     W+K     +   +E+       +L         H D+ + I K   EI      LS          +  +Q K  D   L       +E +K +L + + F   HA   + L  L QT++   S+     D L+    +L S  Q  ++ ++   + +    K++ + +++  P   TS       +   + ++++++  K+ +  +L    + F++ +++L   ++ + +++     K+  R++    L+       EL++   +   LKD  +++ ++ V   P+    V   ++   + WD     + E QG+   LI L  +  + K  V   L++    +  +    I+ + D+     K     + +   +   + F  K   L+ EL+ + + D + ++TDM+    K+  + + ++ N++ L   + +W       D++ GW   +       A NI++L     A+  L + ++++     +  ++ +K+  L E   N   P E LQ    D+ Q   + +     + E   G  K   A R +++    D    L  + E    C       +E  E L     ++ E    + S+ + + E+  L  K  + ++  L + ++ L K+++  S   ++T   + + LEKH+
Sbjct:   18 MQRLQDEQEIVQKRTFTKWINSHLAKRKPPMVVDDLFEDMKDGIKLLALLEVLSGQKLPCEQGHRVKRIHAVANIGTALKFLEGRKIKLVNINATDIADGRPSIVLGLMWTIILYFQIEELTSNLPQLQSLSSSASSVDSMVST-----ETASPPSKRK--VAAKIQGNAKKTLLKWVQHTAGKQMGIEVKDFGKSWRTGLAFHSVIHAIQPELVDLEKVKTRSNRENLEDAFTIAETQLGIPRLLDPEDVDVDKPDEKSIMTYVAQFLTQYPDIHGAGCDGQEDDVVFVGFTNNIALLLGFQRDDRLILKETKVWIEQFERDFTRAQMTESSLQDKYQAFKHFRVQYEMKRKQ-VEHIIQPLQRDGKLTLDQAL---VKQCWERVSSRLFDWHIQLDKSLPAPLGTIGAWLYRAEVALREEITIQQVHEETANTIQRKLEQHKDLLQNTDAHKRAFHEIYQTRSVNGIPMPPDQLEDMAERFHFVSSTSELHLMKMEFLELKYRLLSLLVLAESKLKSWIIKYGRRESVELLLQSYISFIENSKFFEQYEVTYQILKQTADIYVK-AEGSVEEAENVMKFMSEATAQWRNLSVEVRSVRSMLEEVISNWDRYGDTVASLQAWLEDAEKMLSQSEHAKKDFFRNLPHWIQQHTAMNDAGNFLIETCDEIVSRDLKQQLLLLNGRWRELFMEVKQYARADEMDRMKKEYIDVTTTLFGFATEAHRKLSEPLEVSFINVKLLIQDLEDLEKRVPVMDAQYKMIAKKAHLFAKESPQEEANEMLTTMSKLKEQLSKVKECCSP-------LLYEAQQLTVPLEELETQITSFYDSLGKINEILSVLEQEAQSSTLFKQKHQELLASQENCKKSLTLIEKGSQSVQKLVTSSQARKPWDHTKLQKQIADVHHAFQSMIKKTGDWKKHVEANSRLMKKFEESRAELEKVLRVAQEGLEEKG-DPEELLRRHTEFFSQLDQRVLNAFLKACDELTDILPEQEQQGLQEAVRKLHKQWKDLQGEAPYHLLHLKIAVEKDRFSAAVEECRAELEQETK-LAPQEGSEKIIKEHRVFFSDKGPHHLCEKRLQLIEELCGKL----PVQDPVRDTCGACHTALKELKASIDNTYTMLVDDPDKWKDYTSRFSEFSSWVSAKKACLKKIKDEPIDTGNHDEVKHMVDEIRNDITKKGESLSWLKSRLKYLIDISSENEAQKRGDELAELSSSFKALVALLSEVEKLLSNFGECVQYK-----------EIVKSSLEGLISGPQESKEEAEMILDSKNLLEAQQLLLHH--QQKTKMISAKKRDLQEQMEQAQQGGQAGPGQEELRKLESTLTGLEQSRERQERRIQVSLRKWERFETNKETVVRYL----FQTGSSHERFLSFSSLESLSSELEQTKEFSKRTESIATQAENLVKEAAELPLGPRNKRVLQRQAKSIKEQVTTLEDTLEEDIKTMEMVKSKWDHFGSNFETLSIWILEKENELSSLEASASAADVQISQIKVTI---QEIESKIDSIVGLEEEAQS-FAQFVTTGESARIKAKLTQIRRYWEELQEHARGLEGTILGHLSQQQKFEENLRKIRQSVSEFAERLADPIKICSSAAETYKVLQEHMDLCQAVESLSSTVTMFSASAQKAVNRESCTQ----EAAALQQQYEEILHKAKEMQTALEDLLARWQRLEKGLSPFLTWLERCEAIASSPEK--DISADRGKVESELQLIQALQNEVVSQASLYSNLLQLKEALFSVA---SKEDVAVMKLQLEQLDERWGDLPQIISKRMHFLQSVLAEHKQFDELLFSFSVWIKQFLGELQRTSEINLRDHQVALTR-------HKDHAAEIEKKRGEITHLQGHLSQLRSLGRAQDLHPLQSKVDDCFQLFEEASQVVERRKLALAQLAEFLQSHACMSTLLYQLRQTVEATKSMSKKQSDSLK---TDLHSAIQDVKTLESSAISLDGTLTKAQCH-LKSASPEERTSCRATTDQLSLEVERIQNLLGTKQSEADALVALKEAFREQKEELLRSIEDIEERMDRERLKVPTRQALQHRLRVFNQLEDELNSHEHELCWLKDKAKQIAQKDVAFAPE----VDREINGLEATWDDTRRQIHENQGQCCGLIDLVREYQSLKSTVCNVLEDASNVV--VMRATIKDQGDLKWAFSKHETSRNEMNSKQKELDSFTSKGKHLLSELKKIHSGDFSLVKTDMESTLDKWLDVSERIEENMDMLRVSLSIWDDVLSRKDEIEGW---SNSSLPKLAENISNLNNSLRAEELLKELESEVKIKALKLEDLHSKINNLKELTKNPETPTE-LQFIEADLRQKLEHAK----EITEEARGTLKDFTAQRTQVERFVKDITAWLINVEESLTRC-----AQTETCEGLKKAKDIRKELQSQQNSITSTQEELNSLCRKHHSVELESLGRAMTGLIKKHEATSQLCSQTQARIQDSLEKHF 2409          

HSP 2 Score: 60.8474 bits (146), Expect = 2.550e-7
Identity = 83/436 (19.04%), Postives = 187/436 (42.89%), Query Frame = 0

Query: 1887 LSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALN---MNVDAKS----VAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLL-VQAADKGANLSCGQGISDDVDALKSRLQA-------VKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLT 2307
            L L++  L +    +    LKE V+ L+  W +   +   RR+  +E+L  W  + E    +   +   E   +K     D    ++  ++KK+ +EEI  A E+ + +++E+ + L+ A++  + S +  +++ ++ + + LL+    +++ L E  V   +  KN+G L+ W+   + +L +    D    + ++  L  QQ+++  +++         NL E + H         E +++++    L+    N+ A S    + I +   +W    +D    + WLE +E   A           A  EL + + F+ ++   E L  ++  +K       +  +D   +L+  +         ++  VT  + RL+  +   + F+   D I  W+      +   ++  +  + A   ++ QLKA    ++
Sbjct: 7734 LMLVRDALAVYLSAEDISMLKERVELLQRQWEELCHQVSLRRQQVSERLNEWAVFSEKNKELCEWLTQME---SKVSQNGDILIEEMIEKLKKDYQEEIAVAQENKI-QLQEMGERLAKASHESKASEIQYKLSRVKDRWQHLLDLMAARVKKLKETLVAVQQLDKNMGSLRTWLAHMESELAKPIVYDSCNSEEIQRKLNEQQELQRDIEKHSTGVASVLNLCEVLLHDCDACATDAECDSIQQATRNLDRRWRNICAMSMERRLKIEETWRLWQKFLDDYSRFEDWLEVSERTAAFPSSSGVLYTVAKEELKKFEAFQRQVH--ESLTQLELINKQYRRLARENRTDSACSLRQMVHGGNQRWDDLQKRVTSILRRLKHFISQREEFETARDSILVWLTEMDLQLTNIEHFSECDVQA---KIKQLKAFQQEIS 8160          

HSP 3 Score: 60.077 bits (144), Expect = 4.619e-7
Identity = 108/585 (18.46%), Postives = 251/585 (42.91%), Query Frame = 0

Query: 1857 NFPATEEDVQALVN----REEAILQQLDDHRGPVLSLLQKG--LDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLDVSPDDRV-KMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHT----NLQDALNELTQLKRFEEE-------IFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKV 2423
            N P   + V+ L +    +E+  L+Q    +G  +  ++ G  L L  D  A   L+ E+  ++  W        E++K     LK W + +    + L  +   +  L++ +P H H+E+  +    K L+  +G+ T+D L ++  + DAL+   + E  S L + +  ++++  +L  +   + Q + E   +W  F +   EL +W+ + + K++Q    D+  ++ + K+  + Q+++    +   +L+     L     H +  ++++ ++  VK   + L   + A+   + + L        ++ +++ WL   E ++A  +   +     +Q  LNE  +L+R  E+       +    ++L+   D  A  +    I      L  R + + A   +   ++E+  + W+ F + Y   + W+E          + G L   A   EL + +A    + +   +L  ++K    +A     D+A  ++Q V     +  DL     + +  +   I   +EF+     +  WL +++ ++  +       + + ++ + +  QE + N ++I ++
Sbjct: 7595 NIPVPLQQVRMLFDEVQFKEKVFLRQ----QGSYILTVEAGKQLLLSADSGAEAALQAELTDIQEKWKAASMHLEEQKKKLAFLLKDWEKCERGIANSLEKLRMFKKRLSQPLPDH-HEELHAEQMRCKELENAVGRWTDD-LTELMLVRDALAVYLSAEDISMLKERVELLQRQWEELCHQVSLRRQQVSERLNEWAVFSEKNKELCEWLTQMESKVSQ--NGDILIEEMIEKLKKDYQEEIAVAQENKIQLQEMGERL-AKASHESKASEIQYKLSRVKDRWQHLLDLMAARVKKLKETLVAVQQLDKNMGSLRTWLAHMESELAKPIVYDSCNSEEIQRKLNEQQELQRDIEKHSTGVASVLNLCEVLLHDCDACATDAECDSIQQATRNLDRRWRNICAMSMERRLKIEETWRLWQKFLDDYSRFEDWLEVSERTAAFPSSSGVLYTVA-KEELKKFEAFQRQVHESLTQLELINKQYRRLARENRTDSACSLRQMVHGGNQRWDDLQKRVTSILRRLKHFISQREEFETARDSILVWLTEMDLQLTNIEHFSECDVQAKIKQLKAFQQEISLNHNKIEQI 8169          

BLAST of PREDICTED: nesprin-1-like vs. SwissProt
Match: gi|116242809|sp|Q8WXH0.3|SYNE2_HUMAN (RecName: Full=Nesprin-2; AltName: Full=KASH domain-containing protein 2; Short=KASH2; AltName: Full=Nuclear envelope spectrin repeat protein 2; AltName: Full=Nucleus and actin connecting element protein; Short=Protein NUANCE; AltName: Full=Synaptic nuclear envelope protein 2; Short=Syne-2)

HSP 1 Score: 307.375 bits (786), Expect = 8.108e-82
Identity = 223/691 (32.27%), Postives = 351/691 (50.80%), Query Frame = 0

Query:  618 DSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKG-------SDRFGSLDSQYQELRAWL--TTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERLRQL-KSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDDIPAMLNEEAAAVLNQKIEDHKAF---FADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTE---ESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQ-CYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAE 1291
            D HI  ++ EQE  QKK F  WINS LA+   P  I DL   ++ G  LL LLEVL G++LP +KG    +     N+  AL +LR++ IKL+NI+  D++DG P+++LGLIWTIIL+F IE   ++ ++L   Y  P++               +P   +   V  +W++ ARKALL W Q Q      V VTDF  SWR+G AFLAII++++P  +DM S++  SN+  L  AF +AE +L + RLL+PEDVDV  PDEKS+MTYVAQFL +Y + A   G        D  G L  Q ++L+  L  +   ++F++       L    + F + K    +   I   L +L K + QL    EAW  LD+       Q+  W+  L+ ALP    Q   WL + E L++ D   +  + +A  ++ +K+   K+    F   S +   FE    N  ++  +P ++L+ M +R+ +I  K  +  + L+F  +KC ++  +   +SKL  W IKYG  E V+ ++  +  F+         +K F+   D S  ++K GEI      E Q+I++    V +        +  V+S L++VL  W  +  N + L+ C+     E +K+        F   A W   +  L +   FLV  S+  VG  + ++   ++  W +L  + +
Sbjct:   20 DLHI-SLQAEQEDTQKKAFTCWINSQLARHTSPSVISDLFTDIKKGHVLLDLLEVLSGQQLPRDKGSNTFQCRI--NIEHALTFLRNRSIKLINIHVTDIIDGNPSIILGLIWTIILHFHIE---KLAQTLSCNYNQPSLDDVSVVDSSP---ASSPPAKKCSKVQARWQMSARKALLLWAQEQCATYESVNVTDFKSSWRNGMAFLAIIHALRPDLIDMKSVKHRSNKDNLREAFRIAEQELKIPRLLEPEDVDVVDPDEKSIMTYVAQFL-QYSKDAPGTGEEAQGKVKDAMGWLTLQKEKLQKLLKDSENDTYFKKY----NSLLSFMESFNEEKKSFLDVLSIKRDLDELDKDHLQLR---EAWDGLDH-------QINAWKIKLNYALPPPLHQTEAWLQEVEELMDEDLSASQDHSQAVTLIQEKMTLFKSLMDRFEHHSNILLTFENKDEN--HLPLVPPNKLEEMKRRINNILEKKFI--LLLEFHYYKCLVLGLVDEVKSKLDIWNIKYGSRESVELLLEDWHKFIE--------EKEFLARLDTS--FQKCGEIYKNLAGECQNINKQYMMVKSDVCMYRKNIYNVKSTLQKVLACWATYVENLRLLRACFEETKKEEIKEVP------FETLAQWNLEHATLNEAGNFLVEVSNDVVGSSISKELRRLNKRWRKLVSKTQ 666          

HSP 2 Score: 69.3218 bits (168), Expect = 6.407e-10
Identity = 91/436 (20.87%), Postives = 191/436 (43.81%), Query Frame = 0

Query: 1906 LKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALN---MNVDAKS----VAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLL-VQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQW---IPRLEDLVKTWKHFDNGYDEIDG-------WI---EAKMTNMK------GHDNLGDLS-----IDALTGELDQLKALNDNLTQK 2309
            LKE+++ L   W D   R   R++   ++L  W  + E    +   +   E   NK +   D    ++  +++K+  EEI   +E+ L +++++ D L  A+N  + + + D++N +  + + L +    +++ L E      +  KN+  L+ W+ + + +L++    DV  D  ++  L  QQD++  ++Q         N+ + + H +     + E +++++   +L+    N+ A S    + I +   +W    +D    + WL+ AE   A           A  EL + + F+ +I   E+L  ++  +K       +  +D    LK   Q V     +W     R+  +++  +HF N  +E +G       W+   + ++TN++        D +  L+     I   T ++DQL    + L QK
Sbjct: 5887 LKEQIEHLHRQWEDLCLRVAIRKQEIEDRLNTWVVFNEKNKELCAWLVQME---NKVLQTADISIEEMIEKLQKDCMEEINLFSENKL-QLKQMGDQLIKASNKSRAAEIDDKLNKINDRWQHLFDVIGSRVKKLKETFAFIQQLDKNMSNLRTWLARIESELSKPVVYDVCDDQEIQKRLAEQQDLQRDIEQHSAGVESVFNICDVLLHDSDACANETECDSIQQTTRSLDRRWRNICAMSMERRMKIEETWRLWQKFLDDYSRFEDWLKSAERTAACPNSSEVLYTSAKEELKRFEAFQRQIH--ERLTQLELINKQYRRLARENRTDTASRLK---QMVHEGNQRWDNLQRRVTAVLRRLRHFTNQREEFEGTRESILVWLTEMDLQLTNVEHFSESDADDKMRQLNGFQQEITLNTNKIDQLIVFGEQLIQK 6313          

BLAST of PREDICTED: nesprin-1-like vs. SwissProt
Match: gi|292630943|sp|Q6ZWQ0.2|SYNE2_MOUSE (RecName: Full=Nesprin-2; AltName: Full=KASH domain-containing protein 2; Short=KASH2; AltName: Full=Nuclear envelope spectrin repeat protein 2; AltName: Full=Nucleus and actin connecting element protein; Short=Protein NUANCE; AltName: Full=Synaptic nuclear envelope protein 2; Short=Syne-2)

HSP 1 Score: 299.671 bits (766), Expect = 1.612e-79
Identity = 202/669 (30.19%), Postives = 332/669 (49.63%), Query Frame = 0

Query:  624 IRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNY-DEFQKCKAEMKNKQEIYERLRQLKSNK-QLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDDIPAMLNEEAAAVLNQKIEDHKAFFADL---SAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLF 1287
            ++ EQE  QKKTF  WINS LAK  PP  + DL   ++ G  LL LLEVL G++LP +KG    +     N+  AL +L+++ IKL+NI+ AD+V+G P+++LGLIWTIIL+F IE   ++ ++L   Y  P+                T     K     +W+  A+KALL W Q Q  +   V VTDF  SWR+G AFLA+I++++P  +DM SMR  SN+  L+ AF +AE +L + +LL+PEDVDV +PDEKS+MTYVAQFL KY + A   G     S  ++ ++   WLT +   F++M   K      Y +++    + M++  E  E    + S K ++G   E    L   W  +  QV  W+  LD ALP    +   WL   EG++      +    EA  ++  K+   K+        S V   F++    S  +  +P  +L+ M +R+ ++  K  +    L+F + KC ++A L   ++KL  W   Y  +E V+ ++  +  F  + K   +   +F + +++     ++ E   EE   +++ +        NT       ++ L+  L  W  +  +   L+     +F+ L K E  ++        W  ++  L +  +FL+  S   V   + ++   ++  W +  
Sbjct:   25 LQAEQEDTQKKTFTCWINSQLAKHTPPSVVSDLFADIKKGHVLLDLLEVLSGQQLPRDKGSNTFQCRI--NIEHALTFLKNRSIKLINIHVADIVEGNPSIILGLIWTIILHFHIE---KLAQTLSCDYNQPSPEVVSVAASSPTSSPPTKK-CSKAQAQARWQWSAKKALLQWAQEQCARSESVNVTDFKSSWRNGMAFLAVIHALRPDLIDMDSMRHRSNKDNLKEAFRIAEHELKIPKLLEPEDVDVVNPDEKSIMTYVAQFL-KYSKDAPGPGD----STQAKVRDALVWLTLQEKRFQKM--LKDSASETYCNKYHSLLSFMESLNEEKESFIDVLSLKGRMGELNEDESRLRQGWTSLMHQVAAWRAQLDDALPSPLKETEAWLKDIEGVVQEGVPTSQSYSEARTLIQGKLSSFKSLMGSFDYHSDVLMAFQSNAEKS--LPAVPPVKLEEMTRRINNVLGKNFIPL--LEFHDSKCSVLALLDEAKAKLDVWNGTYESKESVEVLLEDWHKFTGEKKFLIQLDASFQKCEEMYKNSARECESIREEYMMLEKNVHSCRQYIHNT-------KATLQRALMSWATFEEDLALLKA----SFD-LTKKEQIKEVPVETLLQWNTKHTSLNEVGSFLIGVSSREVAASISKELRRLNKRWRKFI 664          

HSP 2 Score: 65.0846 bits (157), Expect = 1.468e-8
Identity = 88/437 (20.14%), Postives = 192/437 (43.94%), Query Frame = 0

Query: 1905 FLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALN---MNVDAKS----VAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLL-VQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQW---IPRLEDLVKTWKHFDNGYDEIDG-------WI---EAKMTNMK------GHDNLGDLS-----IDALTGELDQLKALNDNLTQK 2309
             LKE+++ L   W D   R   R++   ++L  W  + E    +   +   E   NK +   D    ++  +++K+  EEI   TE+ L +++++ D L  A++  + + L ++++ +  + + L +    +++ L E      +  KN+  L+ W+ + + +L++    DV  +  ++  L  QQD++  ++Q         N+ + + H +     + E +++++   +L+    N+ A S    + I +   +W    +D    + WL+ AE   A          +A  EL + + F+ +I   E+L  ++  +K       +  +D    LK   Q V     +W     R+  +++  ++F N  +E +G       W+   + ++TN++        D +  L+     I   T ++DQL    + L QK
Sbjct: 5873 VLKEQIQLLHRQWEDLCLRVAIRKQEIEDRLNSWIVFNEKNKELCAWLVQME---NKVLQTADVSIEEMIEKLQKDCMEEISLFTENKL-QLKQMGDQLIKASSKAKAAELEEKLSKINDRWQHLFDVIGSRVKKLKETFAFIQQLDKNMSNLRTWLARIESELSKPVVYDVCDNQEIQKRLAEQQDLQRDIEQHSAGVESVFNICDVLLHDSDACANETECDSIQQTTRSLDRRWRNICAMSMERRMKIEETWRLWQKFLDDYSRFEDWLKSAERTAACPNSSEVLYTNAKEELKRFEAFQRQIH--ERLTQLELINKQYRRLARENRTDTASKLK---QMVHEGNQRWDNLQKRVTAILRRLRYFTNQREEFEGTRESILVWLTEMDLQLTNVEHFSESDAEDKMRQLNGFQQEITLNTNKIDQLIVFGEQLIQK 6300          

BLAST of PREDICTED: nesprin-1-like vs. SwissProt
Match: gi|613504943|sp|Q9QXS1.3|PLEC_MOUSE (RecName: Full=Plectin; Short=PCN; Short=PLTN; AltName: Full=Plectin-1; AltName: Full=Plectin-6)

HSP 1 Score: 227.254 bits (578), Expect = 1.012e-57
Identity = 159/431 (36.89%), Postives = 219/431 (50.81%), Query Frame = 0

Query:  626 DEQERVQKKTFVNWINSYLAK--RIPPMK-IEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISW----------------FEQMQVTKGQ-LPMNYDEFQKCKAEMKNKQEIYERLRQLKSNKQLGITPEAWHELD--NNWQKV-------ETQVR-HWQWL-----LDTALPGEFGQIGEWLNQGEGLINSD 1021
            DE++RVQKKTF  W+N +L K  R    + I DL + LRDG  L++LLEVL G+ LP EKGR+  R H L NV  AL+YLR +++KLVNI   D+ DG P + LGLIWTIIL+FQI  + +V                   +G+SED                  + A++ LL W Q  +    G+   +F  SWRDG  F AII+  +P  +DM  + +++N   L+ AF VAE  LGVTRLLDPEDVDVP PDEKS++TYV+      P    A+   R   L  ++QE R  +   + W                FE++++   Q L     E    +A+    + IY+ L       QL I P  +H LD    W K+       E Q+R  ++ L     + + L  E G   E LNQ + L+ SD
Sbjct:  181 DERDRVQKKTFTKWVNKHLIKHWRAEAQRHISDLYEDLRDGHNLISLLEVLSGDSLPREKGRM--RFHKLQNVQIALDYLRHRQVKLVNIRNDDIADGNPKLTLGLIWTIILHFQI-SDIQV-------------------SGQSED------------------MTAKEKLLLWSQRMVEGYQGLRCDNFTTSWRDGRLFNAIIHRHKPMLIDMNKVYRQTNLENLDQAFSVAERDLGVTRLLDPEDVDVPQPDEKSIITYVSSLYDAMPRVPGAQDGVRANELQLRWQEYRELVLLLLQWIRHHTAAFEERKFPSSFEEIEILWCQFLKFKETELPAKEADKNRSKVIYQSLEGAVQAGQLKIPP-GYHPLDVEKEWGKLHVAILEREKQLRSEFERLECLQRIVSKLQMEAGLCEEQLNQADALLQSD 570          

BLAST of PREDICTED: nesprin-1-like vs. SwissProt
Match: gi|209572726|sp|Q15149.3|PLEC_HUMAN (RecName: Full=Plectin; Short=PCN; Short=PLTN; AltName: Full=Hemidesmosomal protein 1; Short=HD1; AltName: Full=Plectin-1)

HSP 1 Score: 226.868 bits (577), Expect = 1.438e-57
Identity = 157/428 (36.68%), Postives = 214/428 (50.00%), Query Frame = 0

Query:  626 DEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISW----------------FEQMQVTKGQ-LPMNYDEFQKCKAEMKNKQEIYERLRQLKSNKQLGITPEAWHELD--NNWQKV-------ETQVRHWQWLLD------TALPGEFGQIGEWLNQGEGLINSD 1021
            DE++RVQKKTF  W+N +L K      I DL + LRDG  L++LLEVL G+ LP EKGR+  R H L NV  AL+YLR +++KLVNI   D+ DG P + LGLIWTIIL+FQI  + +V                   +G+SED                  + A++ LL W Q  +    G+   +F  SWRDG  F AII+  +P  +DM  + +++N   L+ AF VAE  LGVTRLLDPEDVDVP PDEKS++TYV+      P     +   R   L  ++QE R  +   + W                FE++++   Q L     E    +A+    + IY+ L       QL + P  +H LD    W K+       E Q+R     L+      T L  E G   E LNQ + L+ SD
Sbjct:  175 DERDRVQKKTFTKWVNKHLIK--AQRHISDLYEDLRDGHNLISLLEVLSGDSLPREKGRM--RFHKLQNVQIALDYLRHRQVKLVNIRNDDIADGNPKLTLGLIWTIILHFQI-SDIQV-------------------SGQSED------------------MTAKEKLLLWSQRMVEGYQGLRCDNFTSSWRDGRLFNAIIHRHKPLLIDMNKVYRQTNLENLDQAFSVAERDLGVTRLLDPEDVDVPQPDEKSIITYVSSLYDAMPRVPDVQDGVRANELQLRWQEYRELVLLLLQWMRHHTAAFEERRFPSSFEEIEILWSQFLKFKEMELPAKEADKNRSKGIYQSLEGAVQAGQLKVPP-GYHPLDVEKEWGKLHVAILEREKQLRSEFERLECLQRIVTKLQMEAGLCEEQLNQADALLQSD 559          

BLAST of PREDICTED: nesprin-1-like vs. SwissProt
Match: gi|32699597|sp|Q96JQ2.1|CLMN_HUMAN (RecName: Full=Calmin; AltName: Full=Calponin-like transmembrane domain protein)

HSP 1 Score: 222.246 bits (565), Expect = 2.094e-57
Identity = 120/277 (43.32%), Postives = 169/277 (61.01%), Query Frame = 0

Query:  618 DSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPE 894
            D  +  ++ E+E VQK+TF  WIN +L K  PP++++DL   ++DG  L+ALLEVL G  L  E      R   L+N+  AL++L    +KLV+I+AA++ DG P++VLGLIW IIL+FQI+E T  L          ++     GT        TP   R +++  K +  A KALL W Q +  +K+GV V DF  SWR G AFLA+I +I P  VDM    + S +  LE AF +A+  L + RLL+PED+ V +PDE+S+MTYVAQFL ++PE
Sbjct:   20 DIRVQNLQVERENVQKRTFTRWINLHLEKCNPPLEVKDLFVDIQDGKILMALLEVLSGRNLLHEYKSSSHRIFRLNNIAKALKFLEDSNVKLVSIDAAEIADGNPSLVLGLIWNIILFFQIKELTGNLSRNSPSS---SLSPGSGGTDSDSSFPPTPTAERSVAISVKDQRKAIKALLAWVQRKT-RKYGVAVQDFAGSWRSGLAFLAVIKAIDPSLVDMKQALENSTRENLEKAFSIAQDALHIPRLLEPEDIMVDTPDEQSIMTYVAQFLERFPE 292          

BLAST of PREDICTED: nesprin-1-like vs. SwissProt
Match: gi|677286726|sp|Q91ZU6.2|DYST_MOUSE (RecName: Full=Dystonin; AltName: Full=Bullous pemphigoid antigen 1; Short=BPA; AltName: Full=Dystonia musculorum protein; AltName: Full=Hemidesmosomal plaque protein; AltName: Full=Microtubule actin cross-linking factor 2)

HSP 1 Score: 226.483 bits (576), Expect = 2.401e-57
Identity = 136/328 (41.46%), Postives = 183/328 (55.79%), Query Frame = 0

Query:  609 EQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDS-----QYQELRAWLTTKISWFEQMQVT 931
            EQ+Y +  Y+  + + +DE+++VQKKTF  WIN +L K      + DL + LRDG  L++LLEVL G+ LP EKGR+  R H L NV  AL+YL+ +++KLVNI   D+ DG P + LGLIWTIIL+FQI +                     + TGESED                  + A++ LL W Q       GV   +F   WRDG  F AII+  +P  +DM ++  +SN   LE AFYVAE K+GV RLLDPEDVDV SPDEKSV+TYV+     +P+  V +G +  G+ D      +YQ +  +L   I W     VT
Sbjct:   15 EQEYLQ-AYEDVLERYKDERDKVQKKTFTKWINQHLMK--VRKHVNDLYEDLRDGHNLISLLEVLSGDTLPREKGRM--RFHRLQNVQIALDYLKRRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDI--------------------HVTGESED------------------MSAKERLLLWTQQATEGYAGVRCENFTTCWRDGKLFNAIIHKYRPDLIDMNTVAVQSNLANLEHAFYVAE-KIGVIRLLDPEDVDVSSPDEKSVITYVSSLYDAFPK--VPEGGEGIGANDVEVKWIEYQNMVNYL---IQWIRHHVVT 293          

HSP 2 Score: 61.6178 bits (148), Expect = 1.276e-7
Identity = 193/1053 (18.33%), Postives = 428/1053 (40.65%), Query Frame = 0

Query: 1422 QWCDDVDSLVKTVQAVGE----PEDLQIELAKHKPFLA--KTVN-----MQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRR--------MDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLF-NMKNIN-SPQVVQQHRSHFAP------GCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRL-----HEDKLNKSIKEIE---NKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVD-IYSKSYHFQAEAHNVHNLLNDIQNKSIEDN-FPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLM-RDPK-APPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAV-PQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLDVSPD-DRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNM---------NVDAKSVAIAKDLD---IWNACQNDLLN-IKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDD-------VDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEH 2413
            Q+ D +D ++++++ + E    P  +  E+ K K  +   K+V+     +Q L ++ R   + ++  +EG E     DVS R        M +I G        V++ E  +   M+L +KF     ++V  +K+ ++   ++++    P VV+Q +           G +  L+  + +  +L     E DK  V++    + + WD++++       + E  +     ++D L      ++   NKL   S      E + Q I+E   + +     Q   E L   AE++ +  +   +   ++D L   +  WD + ERI +   +LE       +++    +++ W+      L++      D    E E      L  DV   +  ++ + +  + L+     +   + Q +L  L  R++ ++   +   Q++D    ++  F  E  ++   L D +   +         E  +  +N    +            SL+ +G  ++ R P+ A   + +++  L+  W    S+  E++    E L +   +  +    +  +  +E  LN A  P      +  Q+   K    E+    E I+ ++ +    L   +  +    + + + +++ +   +++   E+ + L E   +  +FH+   +L +W+ +++  L+  ++L+++ D D++K  L        + K+ +K     H++++    T     LK +      +++  NM          +  KSV     L+   +++    D L  +  WL + E ++A   P H ++   +N +   K F++E+ GK    VQA  + A     +G  DD       +  L +R + V A       RLE  ++  + F +    +  W+      ++ H  L D   DAL   ++Q K     L +K+ EL   +   D +      D+   +K  +  ++ +  ++   ++     +   +  +   Q+ +  + +WL+   + + E ++     I   +E V +L  EH
Sbjct: 5871 QFHDKIDQILESLERIAERLRQPPSISAEVEKIKEQIGENKSVSVDMEKLQPLYETLRQRGEEMIARSEGTE----KDVSARAVQDKLDQMVFIWGSIHTL---VEEREAKLLDVMELAEKFWCDHMSLVVTIKDTQDFIRDLEDPGIDPSVVKQQQEAAEAIREEIDGLQEELDMVITLGSELIAACGEPDKPIVKKSIDELNSAWDSLNKAWKDRVDRLEEAMQAAVQYQDGLQGIFDWVDIAGNKLATMSPIGTDLETVKQQIEELKQFKSEAYQQQIEMERLNHQAELLLKKVTEEADKHTVQDPLMELKLIWDSLDERIVSRQHKLEGALLALGQFQHALDELLAWLTHTKGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSEGEEASNLQYKLRILNQRWQDILEKTDQRKQQLDSALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAKEQLNAHMEVCTAFAIKEETYKSLMLRGQQMLARCPRSAETNIDQDITNLKEKWESVKSKLNEKKTKLEEALHLAMNFHNSLQDFINWLTQAEQTLNVASRPSLILDTILFQIDEHKVFANEVNSHREQII-ELDKTGTHLKYFSQKQDVVLIKNLLISVQSRWEKVVQRLVERGRSLDEARKRAKQFHEAWSKLMEWLEESEKSLD--SELEIANDPDKIKAQL-------VQHKEFQKSLGGKHSVYDTTNRTG--RSLKEKTSLADDNLKLDNMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKVFQKEL-GKRTSSVQALKRSAR-ELIEGSRDDSSWVRVQMQELSTRWETVCALSISKQTRLESALQQAEEFHSVVHTLLEWLAEAEQTLRFHGALPD-DEDALRTLIEQHKEFMKRLEEKRAELSKATGMGDALLAVCHPDSITTIKHWITIIQARFEEVLAWAKQHQQRLAGALAGLIAKQELLETLLAWLQWAETTLTEKDK---EVIPQEIEEVKTLIAEH 6898          

BLAST of PREDICTED: nesprin-1-like vs. SwissProt
Match: gi|294862529|sp|Q03001.4|DYST_HUMAN (RecName: Full=Dystonin; AltName: Full=230 kDa bullous pemphigoid antigen; AltName: Full=230/240 kDa bullous pemphigoid antigen; AltName: Full=Bullous pemphigoid antigen 1; Short=BPA; Short=Bullous pemphigoid antigen; AltName: Full=Dystonia musculorum protein; AltName: Full=Hemidesmosomal plaque protein)

HSP 1 Score: 225.713 bits (574), Expect = 3.634e-57
Identity = 133/322 (41.30%), Postives = 181/322 (56.21%), Query Frame = 0

Query:  609 EQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDS-----QYQELRAWLTTKISWF 925
            EQ+Y +  Y+  + + +DE+++VQKKTF  WIN +L K      + DL + LRDG  L++LLEVL G+ LP EKGR+  R H L NV  AL+YL+ +++KLVNI   D+ DG P + LGLIWTIIL+FQI +                     + TGESED                  + A++ LL W Q       G+   +F   WRDG  F AII+  +P  +DM ++  +SN   LE AFYVAE K+GV RLLDPEDVDV SPDEKSV+TYV+     +P+  V +G +  G+ D      +YQ +  +L   I W 
Sbjct:   15 EQEYLQA-YEDVLERYKDERDKVQKKTFTKWINQHLMK--VRKHVNDLYEDLRDGHNLISLLEVLSGDTLPREKGRM--RFHRLQNVQIALDYLKRRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISD--------------------IHVTGESED------------------MSAKERLLLWTQQATEGYAGIRCENFTTCWRDGKLFNAIIHKYRPDLIDMNTVAVQSNLANLEHAFYVAE-KIGVIRLLDPEDVDVSSPDEKSVITYVSSLYDAFPK--VPEGGEGIGANDVEVKWIEYQNMVNYL---IQWI 287          

HSP 2 Score: 67.3958 bits (163), Expect = 2.518e-9
Identity = 185/990 (18.69%), Postives = 410/990 (41.41%), Query Frame = 0

Query: 1412 AFDMGERELLQWCDDVD----SLVKTVQAVGEPEDLQIELAKHKPFLAKTVN-------MQSLLQSKRNVFQGVLKNTEGKE-GLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLF-NMKNIN-SPQVVQQHRSHFAP------GCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRL-----HEDKLNKSIKEIE---NKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVD-IYSKSYHFQAEAHNVHNLLNDIQNKSIEDN-FPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLM-RDPK-APPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAV-PQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLDVSPD-DRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTA-------TVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLD---IWNACQNDLLN-IKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDD-------VDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDL 2349
            A D    +  Q+ D +D    SL + V+ + +P  +  E+ K K  +++  N       +Q L ++ +   + ++  + G +  + A  V  ++D +   +E     V++ E  +   M+L +KF     +++  +K+ ++   ++++    P VV+Q +           G +  L+  + +  +L     E DK  V++    + + WD++++       K E  +     ++D L      ++    KL   S      E + Q I+E   + +     Q   E L   AE++ +  +   +   ++D L   +  WD + ERI     +LE       +++    +++ W+   +  L++      D    E E      L  DV   +  ++ + +  + L+   A +   + Q +LE L  R+++++   E   Q++D    ++  F  E  ++   L D +   +         E  +  +N    +    +       SL+QKG  ++ R PK A   + +++  L+  W    ++  ER+    E L +  E+  +    +  +  +E  LN A  P      V  Q+   K    E+    E I+ ++ +    L   +  +    + + + +++ +   +++   E+ + L +   +  +FH+   +L +W+ +++  L+  ++L+++ D D++K  L        + K+ +K     H++++    T        ++ D  ++++ +  ++      +  KSV     L+   +++    D L  +  WL + E ++A   P H ++   +N +   K F++E+ GK    VQA  + A     +G  DD       +  L +R + V A       RLE  ++  + F +    +  W+      ++ H  L D   DAL   +DQ K     L +K+ EL   +   D V      D+   +K  +  ++ +  ++
Sbjct: 6039 ALDEAISQSTQFHDKIDQILESLERIVERLRQPPSISAEVEKIKEQISENKNVSVDMEKLQPLYETLKQRGEEMIARSGGTDKDISAKAVQDKLDQMVFIWENIHTLVEEREAKLLDVMELAEKFWCDHMSLIVTIKDTQDFIRDLEDPGIDPSVVKQQQEAAETIREEIDGLQEELDIVINLGSELIAACGEPDKPIVKKSIDELNSAWDSLNKAWKDRIDKLEEAMQAAVQYQDGLQAVFDWVDIAGGKLASMSPIGTDLETVKQQIEELKQFKSEAYQQQIEMERLNHQAELLLKKVTEESDKHTVQDPLMELKLIWDSLEERIINRQHKLEGALLALGQFQHALDELLAWLTHTEGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSAGEEASNLQNKLEVLNQRWQNVLEKTEQRKQQLDGALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAKEQLNVHMEVCAAFEAKEETYKSLMQKGQQMLARCPKSAETNIDQDINNLKEKWESVETKLNERKTKLEEALNLAMEFHNSLQDFINWLTQAEQTLNVASRPSLILDTVLFQIDEHKVFANEVNSHREQII-ELDKTGTHLKYFSQKQDVVLIKNLLISVQSRWEKVVQRLVERGRSLDDARKRAKQFHEAWSKLMEWLEESEKSLD--SELEIANDPDKIKTQL-------AQHKEFQKSLGAKHSVYDTTNRTGRSLKEKTSLADDNLKLDDMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKAFQKEL-GKRTSSVQALKRSAR-ELIEGSRDDSSWVKVQMQELSTRWETVCALSISKQTRLEAALRQAEEFHSVVHALLEWLAEAEQTLRFHGVLPD-DEDALRTLIDQHKEFMKKLEEKRAELNKATTMGDTVLAICHPDSITTIKHWITIIRARFEEV 7015          

BLAST of PREDICTED: nesprin-1-like vs. SwissProt
Match: gi|338818272|sp|D3ZHV2.1|MACF1_RAT (RecName: Full=Microtubule-actin cross-linking factor 1; AltName: Full=Actin cross-linking family 7)

HSP 1 Score: 223.787 bits (569), Expect = 1.451e-56
Identity = 128/312 (41.03%), Postives = 179/312 (57.37%), Query Frame = 0

Query:  621 IHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGS--LDSQYQELRAWLTTKISWFEQMQV 930
            + ++ DE++RVQKKTF  W+N +L K      I DL + LRDG  L++LLEVL G +LP EKGR+  R H L NV  AL++L+ +++KLVNI   D+ DG P + LGLIWTIIL+FQI +                     Y +GES D                  + A++ LL W Q       G+  T+F   W DG  F A+I+  +P  VDM  ++ +SN+  LE AF VAE +LGVTRLLD EDVDVPSPDEKSV+TYV+     +P+  V +G +   +  +DS++QE ++ + + I W  Q  +
Sbjct:   69 VVRVADERDRVQKKTFTKWVNKHLMK--VRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRM--RFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDI--------------------YISGESGD------------------MSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAE-RLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPK--VPEGGEGISATEVDSRWQEYQSRVDSLIPWIRQHTI 335          

BLAST of PREDICTED: nesprin-1-like vs. nr
Match: gi|1325268826|ref|XP_023347934.1| (nesprin-1-like [Eurytemora affinis])

HSP 1 Score: 2446.77 bits (6340), Expect = 0.000e+0
Identity = 1218/2648 (46.00%), Postives = 1786/2648 (67.45%), Query Frame = 0

Query:  610 QDYAKHN---YDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGA----------VDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERLRQL-KSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDDIPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAVGEPEDLQIELAKHKPFLAKTVNMQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEA-----HNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKLNQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAK--MTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAK--VEINAKN-----KILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLEQTL-DNVTSLDGLEIALAELDSLAQQCQSKKNDGANDEEMAAKSKTYVI--QNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQMVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKANVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKCLDRDKKEVMDEIAKLKIDISELLLSWRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNKGAQMDAINEY 3226
            QDY   +   + + I  ++DEQER+Q KTFVNWINSYL KR PPMK+E+LID L+DG RLLALLEVL GE+LP E+GRVLRRPH+LSN NTALE+L+SKRIKLVNINA+DVVDGRPAVVLGLIWTIILYFQIEENTRVLE LGQR+ SP+V + GY +   +D     G V K SV EKWK+GA+KALL W Q+Q+G+  G  V DFG SWRDGNAF+A++NSI+PG           VD+ SMR   N+ RL+TAF VA+ +LG+ RLLDPEDVDV  PDEKS+MTYVAQFLH+YPEG+  KG DRF S++SQY+ L+ WL T++  F+ M  T+GQ  MNY+E+ +   E K K+ ++ERL+QL +S  Q+GI+ +AW ELD NWQK+  QVRHWQWLLDT LPGEFGQIGEWLNQGEGLI  DDIP  LNEEAAAVLNQKIEDHK+FF+D+++VQ QF T +++SP V QIP++QL+S+  RL+ IGP+A++R+V+LK+LEHKCCIVAFL+LTESKLKNWTIKYG EE+V+QIM+QY+TFVSKNKIFQEFQKA++EFQ V  EY+KDG I   E++ ID+FI E+  RWK TSTELRCVQSLLEEVL +W+RWN      + YI+ A+E L K E+ Q EFF D ++WKE+Y+LL DT AFL+AT D +VG +++ +  I+ NNWEQLF   E F+ +GDISR ++ YQ+GLE+LD WLR  E VLN +QQV+S S+++ LE LM  H EVG ME+LFK +SRKFQ LVPEL +DDIE MMF LKK+KENLVI+RS+IPT+IQL+H +L+Q++A D GE ++  WC + +S++  +   G  E LQ +L  H+ F  KTVNMQ++LQSK NVFQ +LKNTEGKEG+D SD+  RM  +N  F   +   +Q E  +   ++ W +FLD Q  ++ W+++A+ L  +K+I S + V+ H++ F    + ++  ++  AQ LE F+ E++K ++  +   ++ +W  +  +AP H +K EFRL ED  +K +KE+E +L  ES +F K+E++  I+++HT +F  + ++      L  ++ + + +    PE+  L++     +  WD +  RIQ +  +L+QIPEQWREYE++FS MV WMD +D SL+KMFK  + A  FE EK  F E+C DV+ R++DMKWLVQ+LD LV+ RAD+ GLSEQKRLEGLI+RYKSMIPVIE TM +++IYS+SY F+AE        V ++L  I      ++FP +EE V  L+  +E ++ +L+  R  +LSLLQ+G DL+R+  AP FLKE+V+ LE+ WNDCY   +   K   E  KVW+ YK  K  M++L++++E EL K +P+H+HK ++  L++ K+++E+I + ++D++ KMREL+D L+T A+ EQQ+  + EM  +E +L DLL +CDEKI+HL +L++KWT F+KNL E+K ++  AK  L+QIT L++SP+DR+KMT ELQ  VK KMK LE LE DA  LF        + + K+EV TVKKDV  L+  VD ++  +++DL  W   ++ +  +KPWLE+AE+K+A+GL R  ++ ++ N     K+F +E    +  + +  +    + C    +++VDA KSR QA K    QW  ++E LV +W HF+   +++  WIE+K  +   K  D   D+  ++L   L  +K    ++++KQ +++ L++D D V  +++Q+ +  ++ +V  LK ++S L+++++ K+  + E +   QEFQ  + +   W+ +++ +I +LNE+P +  +++LE  H L+QE  D +  + +  VE+N  N     + L+   +A+ +K+     LLE+KK SL RW SF  WH+ES S L H++Q++  + TS D LE    E++++A QCQ++K +G++DEE + KS TY+I  +  KP S+LLLVA+ILQ IV+LK +I+ K+ K   LE KW+ F+  EQ LA+WLQ +L KVQKINV+ S ++ L+ A++AV  L    ++   LK +Y  LGR L+  DP Q+  VQDA++EA SKW KVT+LL EQQ KSQ+LI++W+QCL +K  V  +L+E    +E L     ++  D A+ VD+C++    LKK R PFE +YK+QTQLI ELQTVP FD +PL+ ++ +VQ+K+ +LG+ L   LN L+S++V+W Q EQ  DD+ GW+ D +   + A  N+ D + AK +L K+ ++L S +  KA++  K+  +   N  K I  L   +  +D      +   + L+ A+  L +++  I+ ++KA  ++L  +RE+ + CED +G D +I +RL     +Q E  ++EE +  IE +IK +  ++ + D   LAK+ + L K+YD V+SQ  K    LY+VLEKHYV+KV+   K   + KEK+ WC P+  GDKFS E K+D+L ++   ++  +     L     +++++ D+ +  E+++T+  L+     + +EI+K++I + + +  WR  +L ++ L  WLK +E   R         E  ++ + KL ++++E+  + +   EL++ ++ +M  +P++RV QQT  + +RY     ++   +++L  + +NK  Q DA+N Y
Sbjct:  208 QDYVDEDLAAFQAQISHLQDEQERIQMKTFVNWINSYLRKRKPPMKVENLIDDLKDGVRLLALLEVLSGEKLPMERGRVLRRPHFLSNCNTALEFLKSKRIKLVNINASDVVDGRPAVVLGLIWTIILYFQIEENTRVLEKLGQRFSSPSVEKRGYASSTDDDRSLREGSVGKRSVQEKWKVGAKKALLQWVQSQLGKNIGFQVNDFGPSWRDGNAFIAVVNSIRPGYQINTEHYQGLVDVESMRTVENRVRLDTAFTVADTELGIARLLDPEDVDVDRPDEKSIMTYVAQFLHRYPEGSEGKGEDRFASIESQYKILQDWLLTQLQVFDTMHQTRGQASMNYNEYTRVLTEYKEKEIVHERLKQLIESQAQVGISADAWTELDTNWQKIAAQVRHWQWLLDTGLPGEFGQIGEWLNQGEGLIYGDDIPTQLNEEAAAVLNQKIEDHKSFFSDITSVQKQFATIISSSPLVAQIPREQLESIESRLKGIGPRAEMRSVKLKYLEHKCCIVAFLVLTESKLKNWTIKYGSEEKVKQIMTQYRTFVSKNKIFQEFQKAYIEFQQVCEEYRKDGGITRIETEQIDKFIKEIGERWKGTSTELRCVQSLLEEVLQHWQRWNSTVPEFERYIVTAYEMLNKGEEEQSEFFGDISSWKEKYVLLTDTAAFLLATCDQSVGLEIKSRMDIIINNWEQLFGFVEKFMHAGDISRNRQEYQKGLEKLDTWLRHVEEVLNLSQQVKSESMRQMLERLMVFHGEVGEMEDLFKGISRKFQNLVPELTSDDIEEMMFVLKKEKENLVIVRSLIPTKIQLYHQILTQLDALDTGEADIHLWCSEANSVISGLSGKGNREQLQHQLDTHRNFFVKTVNMQAMLQSKNNVFQSMLKNTEGKEGIDVSDLKTRMGKLNEVFASTIDDSRQLELKLQEALKSWGRFLDQQTKVMKWIQDAQILIAVKHIESKENVETHKAFFIENNDKLMQEFVNAAQDLEKFILEAEKVQLSGNISRLQEKWTEIQSFAPLHMMKVEFRLDEDTFSKYMKEVEKELNSESNSFHKNEDVASILKQHTAFFKSSGLLAKIEACLSNLSRLSRTFSEKMPENPVLQESYIKHKEHWDSLLSRIQGLYNQLQQIPEQWREYEERFSSMVGWMDMIDASLSKMFKSISTAEEFEVEKMNFNEICFDVEQRKEDMKWLVQQLDQLVAHRADNEGLSEQKRLEGLISRYKSMIPVIETTMSRIEIYSRSYSFRAEVAKETKSGVISILERIHISGSRESFPDSEEAVNLLIGSQEEMISELEKQRSSILSLLQRGKDLIREVNAPDFLKEDVRGLEAKWNDCYGYAMNSLKRLKETQKVWNSYKCQKSVMVKLLEEAETELQKLIPKHNHKAIQSDLKINKDMREDIKRTSDDLMIKMRELSDTLATVASKEQQNEFAKEMAELEARLNDLLAQCDEKIKHLVDLNIKWTNFNKNLVEMKVFIENAKKNLSQITSLEMSPEDRLKMTKELQNQVKEKMKTLENLEKDAQFLFAGSTDIYEIKEFKVEVATVKKDVTELHGEVDEQTSKVSQDLQHWVRYKSAIGTVKPWLEQAEVKMAVGLTRPFSISESRNIYDDAKQFNKEADDTKIKIDEIEEMSKLIKCKTSAANEVDAFKSRWQAAKTIAEQWSQKMEILVDSWNHFNLLMEDLKAWIESKEEIIIRKIDDKNNDM--ESLMNALGAMKETLQDISKKQADIITLTRDGDKVGSHLNQEGSCRIRAEVADLKQRISTLAETAQHKIEFVSEALNAKQEFQSKLDNFNEWIAEISQKIDKLNEIPVDKTEAALEKTHILSQEIHDKKPMLERMQVEVNNSNGKENSEELTVQFKAILRKHSSALKLLEDKKASLTRWISFLNWHSESMSHLRHIQQSVYSHQTSPDELETIAGEMENIAVQCQTRKIEGSDDEEASMKSNTYIIERETQKPMSILLLVAEILQNIVKLKKLIDDKKGKQQDLESKWEEFRTAEQNLAEWLQVILSKVQKINVKNSNMEALEEASTAVTSLLKDNNKNEHLKMEYRELGRFLMQHDPSQMKAVQDAVTEAESKWTKVTNLLTEQQSKSQTLISMWKQCLESKQGVSARLEEADDILESLNEVVPQNTVDAARHVDRCKESFSALKKTRQPFEAYYKRQTQLISELQTVPGFDTSPLKKELSQVQQKFGFLGEGLTKKLNNLDSQLVIWKQLEQQTDDIFGWLADAKSNMKDALANVTDSDIAKIRLEKFRSELNSNLCSKASIETKIGQIKSLNQNKEIPSLNKLVATIDDEIRETKRLSSDLENALGNLGESSLMIKEEVKATIEELNKIREELLKCEDTSGSDEQIYDRLKKSTDIQEELSNYEEKIAVIEDKIKSVHEEYGSGD-NTLAKDFAILEKKYDAVNSQCTKVISVLYSVLEKHYVDKVKELTKFNNNFKEKISWCLPEPSGDKFSTECKLDSLKDIDVTVKNMKPVLQELEICGKVIIRIVDEQRMEEIQNTMNLLNDQINFIENEISKIRIVLEKNIEIWRKYELSTECLNSWLKETEDTIRLATNSHVSFENFEDENQKLTRIQSEMSRKSEEFTELSKVSNEIMVENPESRVEQQTQSINSRYALATNALAVHIEKLSKIFENKDMQKDAVNVY 2852          

BLAST of PREDICTED: nesprin-1-like vs. nr
Match: gi|1339073108|ref|XP_023723811.1| (uncharacterized protein LOC111873382 isoform X1 [Cryptotermes secundus])

HSP 1 Score: 1769.98 bits (4583), Expect = 0.000e+0
Identity = 977/2665 (36.66%), Postives = 1587/2665 (59.55%), Query Frame = 0

Query:  578 SRPNSALANPESQEWYDKYQLKAFPHDSQFGEQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERL-RQLKSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDDIPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAVGEPEDLQIELAKHKPFLAKTVNMQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVP-QHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGK-AKGKLNQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFE---EEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWI-EAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKV--EINAKN-------KILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLEQTLDN-VTSLDGLEIALAELDSLAQQCQSKKNDGANDEEMAAKSKTYV--IQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQMVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKANVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKCLDRDKKEVMDEIAKLK---IDISELLLS----WRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNK 3216
            SRP S  +   S EWY +Y+ ++F + +   E   ++  YDSHI +I+DEQERVQKKTFVNWINSYL+KR+PP++I+DLID L+DGTRLLALLEVL GERLP E+GR L+RPH+LSN NTAL++L+SK+IKLVNIN++D+VDGRP VVLGLIWTIILYFQIEENTR LESL   +G  A      G   SE   G     +K    E+ + GAR+A+LHW  + + +  G+ V DFG SW+DG AFLAII++I+   ++  +MRQ SN+TRLETAF VAE +LG+ RLLDPEDVDVP PDEKS+MTYVAQFLHKYPE   A GS   G+++++Y EL +WL  K  + E +Q T   L M Y +++  K E   K +++ +L R ++S   + IT E+W E++  W K+ETQ+ +W WLLD+ LPG+ GQ+GEWL + E LI SDDIP ++NEE A+++++K+E+HK FFADL A+Q  F+ A  +   V+ +P +QL+S+ +R   IGP+A  R VRLKFLEHKCC++AFL LTE+KL+ WT+KYG+EE+VQQ++ QY+ FVS+N+IFQEF KA+++ Q V  EY+++G ID  ES SIDRF+ E   RWKN S ELRCVQS+LEEV+ YW+RWN   +  + ++  A+  L   E+ + E+F D + WK+++ LL DTV+FL+AT +  V  +LRE+++ +S  WE LF+  + ++ +GDI R ++ Y+ G+E+L QWLR AES+L+++Q   +  IK   E L +L +E+ G+EELFK++S+KFQ ++ +L  D+++ +M +LKK+KE LV +R++IP ++ LFH +L Q E+ + G+ E+ QW D+ ++L+ +       E +Q +L KHK F ++T+  +S+L+SK  VF+ ++K+ +  EG+D  D+  RM  +N RF    +  QQ+E  +  G++ W  F + ++ I  WL++A+ L   ++I++ Q V+ H++ F    E  + + +  AQ L   L       V    G ++A+W  V  +AP H ++ EFRL E   N+ +KEIE ++  E QAF K E++  I+  + ++F    ++ +    L  M++I   +    P D +L +       QW+    +I+ + ++L+QIP  W+ Y++KF +MV WMD VD +L  + K       F+ E+  FQ +C +VD +R+DMKWLVQ LDSLVS  +D   L+EQK+LE LI RYK +IP IE+TM K D++ K Y ++ E   V  LL  ++  S+    P T + V  ++ ++E  + QLD  R  ++SLLQ+G DL++D  AP F+K EV  LE+ WN+ Y + VE+ K      KVW  YKE K  +L+L++ +E EL +  P  HD ++V   L+ K+++   + KATE++L K+R+L   LST   PE++  L  E+  +E++L   LE  +E++ +L +   +W  F   L EL+ W  + A   L Q+   +++P++RVK +  LQ  ++ K+  L+ L  +A  L E          LK EV  ++  V  L+  V  +  ++ +DL  W   +  L  +KPW+E+AE++V +G+P+  +LQ+A + L+  + FE   EE   K   L   A     ++C     D+VDA+ SR   V     QW  RLE LV TW+ F+N   +ID WI E +    +   NL     D L  EL +LK LN  +++KQ++L+ L++  D ++  ++ ++   VK +V  +K +++ L++  R +++ + + I   QEFQ  + D  +W+  L++ + +++EV    +DS+L++VH+L QEH++ Q     +  E+   N         ++ S   +  KY DL D L+ KK++L++WS   +W+A++   LNH++  L++     + +E    EL+ +  +  S  +  +  + ++ +S TY+     GK  + + L+ ++  ++  L+  +  K + L  +  +W  F+ V++ L++ L  +   + +  +   T   L+ A   +A L  +   K   KD+  RLG+ L+  D   +S VQ  L+   + W+KV+ +LKEQ+ KS  +   W+     K+ V + + +  +  E ++  P     + A   DK +K L+ LKK +   +V   K   L+++ + +  F+ A +  ++ E    +     TL   +  LE+++++W Q ++  ++L  W+ +T D    A+ +++D+E+ + KL++Y ++LP+  + K ++ +K A LV+ N  K I  L++  + ++  FA+V+     L++    + +   ++R  +K   D +  +RE  + C+D TG +S+ILERL     L+ E  +F  ++  ++ +I+++   F A   + L+KELSSL  RYD VSS   KT   L   L K+++EK  +  + +A+ KEK+ WC P+   D+++LE K+ +L ++   L   +   + L    DLL  V   +K  E++     L+RDK  ++ E+  LK   ++  +LL      W+  +L+S+ +A WLK +E   R E      L T  +   ++E  + ++   +  +++++   + +M  +P++RV      L +RY          ++RL  L   K
Sbjct:  268 SRPGSTSS---SIEWYSEYRTQSFHNMAAKLEYIRSRSQYDSHIAEIKDEQERVQKKTFVNWINSYLSKRVPPLRIDDLIDDLKDGTRLLALLEVLSGERLPVERGRNLKRPHFLSNANTALQFLQSKKIKLVNINSSDLVDGRPPVVLGLIWTIILYFQIEENTRALESLDHAFGGSASSLESLGARSSE---GVAVEGKKKEPNERRRQGARRAMLHWVTSTLPKDHGIEVRDFGGSWQDGFAFLAIIDAIKSNLINFPAMRQASNKTRLETAFNVAESELGIARLLDPEDVDVPQPDEKSIMTYVAQFLHKYPEPRAADGSSTLGAIEAEYNELISWLLKKTQYLEHLQQTNS-LSMVYSDYKTFKGEFDEKAKVFGKLKRVIESQSMVTITVESWREIERLWTKLETQLHNWLWLLDSGLPGDLGQVGEWLGRAEALIASDDIPTIMNEETASIISRKLEEHKVFFADLPAIQANFQRAKLSHDAVD-VPSEQLESLTERFEMIGPRAAQRRVRLKFLEHKCCLIAFLHLTETKLRGWTVKYGQEEKVQQLLEQYRNFVSRNRIFQEFNKAYIDMQQVVEEYRREGNIDQRESVSIDRFMHETGERWKNVSMELRCVQSMLEEVVAYWRRWNTLAEEFESWLDRAYGLLDLPEEDKMEYFQDVSVWKDKFQLLGDTVSFLIATCEDQVAHELRERYLRISARWEDLFQHVKQYMHAGDILRHRKDYRAGVERLQQWLRNAESILSSSQLSSTEKIKIYGEQLQQLQNEIEGIEELFKNVSKKFQAMIQDLSRDEVDRIMNTLKKEKEALVRVRALIPMQLHLFHQILVQQESLEAGQNEIGQWLDEAEALIASHSLAAGKEPVQAQLDKHKTFFSRTLYYKSMLESKNKVFRSIVKSVDHNEGIDTGDLKLRMAQLNERFIRVTQLAQQWELKLQEGIRCWHNFRENERIITEWLQKAEKLIAERHIDTKQTVESHKNFFEKVNEKWIQDLVNSAQDLRNCLPYEQHPPVDAAVGRLQAKWKEVLSFAPLHLMRLEFRLDEAAFNQYLKEIEKEIHSEQQAFNKHEDVEAILTRNREFFVECKVVVEIERCLENMSKISNNFNQWDPHDSSLREAHGRAVAQWEGASRKIETISQQLQQIPTLWKNYQQKFLEMVHWMDKVDINLQNIVKEVNTLEEFDKERAVFQSICCEVDAKREDMKWLVQTLDSLVSHCSDKESLAEQKKLEQLIARYKVLIPTIELTMVKTDVHYKCYTYRKEVREVVGLLQKVKETSMSGPQPETLDSVNVMIKQQETAVTQLDHQRSNIMSLLQRGKDLVKDTHAPSFIKSEVSNLETGWNEAYGQTVEKLKKLKGTQKVWTNYKEQKDEILKLLEQAEGELKRVAPGAHDSQKVAADLQAKQDMSVALRKATEEMLRKLRDLCSTLSTMTAPERKPLLQKEVTEIERRLHVTLETVEERVIYLEQFSARWIRFQAQLDELRHWTHQSAPVLLEQLQATELTPEERVKKSQALQAQLEDKINVLDVLSGEAKELLEEDGENVEAAQLKAEVMALRDSVLGLHQGVLMQISSVGQDLAHWQDYKTGLQEVKPWIEEAEVRVNIGIPKPMSLQEAQHLLSSTRTFEKQCEEYLAK---LQGLAALSQQITCRTNAPDEVDAVHSRWAGVHDVAVQWGTRLEKLVGTWEEFENEAKKIDTWIQEGQKAIFETPVNLSTPDKDKLEKELVKLKTLNKEISEKQMQLITLTQVSDNISQGLAMESGTAVKGQVNEMKAKITKLAEDVRQRLNAVSDTILARQEFQGKMEDFENWMDQLHTGVGQVDEVTLAQVDSALQSVHALLQEHSEKQPAFNAIYDEVRKLNCSSPEEASSVNESYSELVTKYQDLEDTLQEKKRALEKWSDLLSWYADTSGQLNHIKYQLESQKLQPEDVERVAEELEVINLKTASWTDVASIIDGVSQQSGTYIRDTATGKRVTAVALLHELETKVNVLQAQLSNKREVLEEVGARWAQFQRVQENLSEGLLSMQGTLHETVMEVDTCGKLEQAGKIIAGLLEEHKLKQGAKDELHRLGKLLMEEDQSNMSEVQTILASLDANWEKVSEMLKEQKVKSLEMSTAWKHFCDAKEKVYSGITDAEKLYELVQEVP-SDLTEAALSHDKTKKALELLKKAKLSLDVMDNKAQILLKQAEPISGFNSAVIGEELTEAHNAWQNAYDTLTKKVQVLEAQLIVWKQIDESKNELVQWLGETSDALLNASQDLSDVESGQSKLNRYKDELPAFYNLKTSLISKTAQLVKLNDGKQIPTLESLNKLLEDEFAHVKSIADKLEDITCAVGEQERSVRDDMKNASDTITKIREAVIACDDLTGENSKILERLKNCQALKNELQNFSSNLELLKKKIEEMKSSFPAFGDSGLSKELSSLQIRYDGVSSHANKTESTLLAFLNKYHMEKFGALQRGVAAHKEKVAWCLPEAGSDRYNLEVKVSSLQDVEVGLMDCETKKTDLDVSLDLLQNVETPEKIKELQ-----LERDK--LVTELESLKNSYLNTKQLLEHNISLWQKYELMSENVASWLKETEGKVRTESVIQLNLSTIDDKIKEMESFQKQVKDFEPEIKDISILGEEIMKENPESRVGHYVGHLVSRYHGVVKFAAMYMERLTSLNKTK 2913          

BLAST of PREDICTED: nesprin-1-like vs. nr
Match: gi|1339073110|ref|XP_023723812.1| (uncharacterized protein LOC111873382 isoform X2 [Cryptotermes secundus])

HSP 1 Score: 1769.59 bits (4582), Expect = 0.000e+0
Identity = 977/2665 (36.66%), Postives = 1587/2665 (59.55%), Query Frame = 0

Query:  578 SRPNSALANPESQEWYDKYQLKAFPHDSQFGEQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERL-RQLKSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDDIPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAVGEPEDLQIELAKHKPFLAKTVNMQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVP-QHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGK-AKGKLNQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFE---EEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWI-EAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKV--EINAKN-------KILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLEQTLDN-VTSLDGLEIALAELDSLAQQCQSKKNDGANDEEMAAKSKTYV--IQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQMVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKANVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKCLDRDKKEVMDEIAKLK---IDISELLLS----WRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNK 3216
            SRP S  +   S EWY +Y+ ++F + +   E   ++  YDSHI +I+DEQERVQKKTFVNWINSYL+KR+PP++I+DLID L+DGTRLLALLEVL GERLP E+GR L+RPH+LSN NTAL++L+SK+IKLVNIN++D+VDGRP VVLGLIWTIILYFQIEENTR LESL   +G  A      G   SE   G     +K    E+ + GAR+A+LHW  + + +  G+ V DFG SW+DG AFLAII++I+   ++  +MRQ SN+TRLETAF VAE +LG+ RLLDPEDVDVP PDEKS+MTYVAQFLHKYPE   A GS   G+++++Y EL +WL  K  + E +Q T   L M Y +++  K E   K +++ +L R ++S   + IT E+W E++  W K+ETQ+ +W WLLD+ LPG+ GQ+GEWL + E LI SDDIP ++NEE A+++++K+E+HK FFADL A+Q  F+ A  +   V+ +P +QL+S+ +R   IGP+A  R VRLKFLEHKCC++AFL LTE+KL+ WT+KYG+EE+VQQ++ QY+ FVS+N+IFQEF KA+++ Q V  EY+++G ID  ES SIDRF+ E   RWKN S ELRCVQS+LEEV+ YW+RWN   +  + ++  A+  L   E+ + E+F D + WK+++ LL DTV+FL+AT +  V  +LRE+++ +S  WE LF+  + ++ +GDI R ++ Y+ G+E+L QWLR AES+L+++Q   +  IK   E L +L +E+ G+EELFK++S+KFQ ++ +L  D+++ +M +LKK+KE LV +R++IP ++ LFH +L Q E+ + G+ E+ QW D+ ++L+ +       E +Q +L KHK F ++T+  +S+L+SK  VF+ ++K+ +  EG+D  D+  RM  +N RF    +  QQ+E  +  G++ W  F + ++ I  WL++A+ L   ++I++ Q V+ H++ F    E  + + +  AQ L   L       V    G ++A+W  V  +AP H ++ EFRL E   N+ +KEIE ++  E QAF K E++  I+  + ++F    ++ +    L  M++I   +    P D +L +       QW+    +I+ + ++L+QIP  W+ Y++KF +MV WMD VD +L  + K       F+ E+  FQ +C +VD +R+DMKWLVQ LDSLVS  +D   L+EQK+LE LI RYK +IP IE+TM K D++ K Y ++ E   V  LL  ++  S+    P T + V  ++ ++E  + QLD  R  ++SLLQ+G DL++D  AP F+K EV  LE+ WN+ Y + VE+ K      KVW  YKE K  +L+L++ +E EL +  P  HD ++V   L+ K+++   + KATE++L K+R+L   LST   PE++  L  E+  +E++L   LE  +E++ +L +   +W  F   L EL+ W  + A   L Q+   +++P++RVK +  LQ  ++ K+  L+ L  +A  L E          LK EV  ++  V  L+  V  +  ++ +DL  W   +  L  +KPW+E+AE++V +G+P+  +LQ+A + L+  + FE   EE   K   L   A     ++C     D+VDA+ SR   V     QW  RLE LV TW+ F+N   +ID WI E +    +   NL     D L  EL +LK LN  +++KQ++L+ L++  D ++  ++ ++   VK +V  +K +++ L++  R +++ + + I   QEFQ  + D  +W+  L++ + +++EV    +DS+L++VH+L QEH++ Q     +  E+   N         ++ S   +  KY DL D L+ KK++L++WS   +W+A++   LNH++  L++     + +E    EL+ +  +  S  +  +  + ++ +S TY+     GK  + + L+ ++  ++  L+  +  K + L  +  +W  F+ V++ L++ L  +   + +  +   T   L+ A   +A L  +   K   KD+  RLG+ L+  D   +S VQ  L+   + W+KV+ +LKEQ+ KS  +   W+     K+ V + + +  +  E ++  P     + A   DK +K L+ LKK +   +V   K   L+++ + +  F+ A +  ++ E    +     TL   +  LE+++++W Q ++  ++L  W+ +T D    A+ +++D+E+ + KL++Y ++LP+  + K ++ +K A LV+ N  K I  L++  + ++  FA+V+     L++    + +   ++R  +K   D +  +RE  + C+D TG +S+ILERL     L+ E  +F  ++  ++ +I+++   F A   + L+KELSSL  RYD VSS   KT   L   L K+++EK  +  + +A+ KEK+ WC P+   D+++LE K+ +L ++   L   +   + L    DLL  V   +K  E++     L+RDK  ++ E+  LK   ++  +LL      W+  +L+S+ +A WLK +E   R E      L T  +   ++E  + ++   +  +++++   + +M  +P++RV      L +RY          ++RL  L   K
Sbjct:  268 SRPGSTSS---SIEWYSEYRTQSFHNMAAKLEYIRSRSQYDSHIAEIKDEQERVQKKTFVNWINSYLSKRVPPLRIDDLIDDLKDGTRLLALLEVLSGERLPVERGRNLKRPHFLSNANTALQFLQSKKIKLVNINSSDLVDGRPPVVLGLIWTIILYFQIEENTRALESLDHAFGGSASSLESLGARSSE---GVAVEGKKKEPNERRRQGARRAMLHWVTSTLPKDHGIEVRDFGGSWQDGFAFLAIIDAIKSNLINFPAMRQASNKTRLETAFNVAESELGIARLLDPEDVDVPQPDEKSIMTYVAQFLHKYPEPRAADGSSTLGAIEAEYNELISWLLKKTQYLEHLQQTNS-LSMVYSDYKTFKGEFDEKAKVFGKLKRVIESQSMVTITVESWREIERLWTKLETQLHNWLWLLDSGLPGDLGQVGEWLGRAEALIASDDIPTIMNEETASIISRKLEEHKVFFADLPAIQANFQRAKLSHDAVD-VPSEQLESLTERFEMIGPRAAQRRVRLKFLEHKCCLIAFLHLTETKLRGWTVKYGQEEKVQQLLEQYRNFVSRNRIFQEFNKAYIDMQQVVEEYRREGNIDQRESVSIDRFMHETGERWKNVSMELRCVQSMLEEVVAYWRRWNTLAEEFESWLDRAYGLLDLPEEDKMEYFQDVSVWKDKFQLLGDTVSFLIATCEDQVAHELRERYLRISARWEDLFQHVKQYMHAGDILRHRKDYRAGVERLQQWLRNAESILSSSQLSSTEKIKIYGEQLQQLQNEIEGIEELFKNVSKKFQAMIQDLSRDEVDRIMNTLKKEKEALVRVRALIPMQLHLFHQILVQQESLEAGQNEIGQWLDEAEALIASHSLAAGKEPVQAQLDKHKTFFSRTLYYKSMLESKNKVFRSIVKSVDHNEGIDTGDLKLRMAQLNERFIRVTQLAQQWELKLQEGIRCWHNFRENERIITEWLQKAEKLIAERHIDTKQTVESHKNFFEKVNEKWIQDLVNSAQDLRNCLPYEQHPPVDAAVGRLQAKWKEVLSFAPLHLMRLEFRLDEAAFNQYLKEIEKEIHSEQQAFNKHEDVEAILTRNREFFVECKVVVEIERCLENMSKISNNFNQWDPHDSSLREAHGRAVAQWEGASRKIETISQQLQQIPTLWKNYQQKFLEMVHWMDKVDINLQNIVKEVNTLEEFDKERAVFQSICCEVDAKREDMKWLVQTLDSLVSHCSDKESLAEQKKLEQLIARYKVLIPTIELTMVKTDVHYKCYTYRKEVREVVGLLQKVKETSMSGPQPETLDSVNVMIKQQETAVTQLDHQRSNIMSLLQRGKDLVKDTHAPSFIKSEVSNLETGWNEAYGQTVEKLKKLKGTQKVWTNYKEQKDEILKLLEQAEGELKRVAPGAHDSQKVAADLQAKQDMSVALRKATEEMLRKLRDLCSTLSTMTAPERKPLLQKEVTEIERRLHVTLETVEERVIYLEQFSARWIRFQAQLDELRHWTHQSAPVLLEQLQATELTPEERVKKSQALQAQLEDKINVLDVLSGEAKELLEEDGENVEAAQLKAEVMALRDSVLGLHQGVLMQISSVGQDLAHWQDYKTGLQEVKPWIEEAEVRVNIGIPKPMSLQEAQHLLSSTRTFEKQCEEYLAK---LQGLAALSQQITCRTNAPDEVDAVHSRWAGVHDVAVQWGTRLEKLVGTWEEFENEAKKIDTWIQEGQKAIFETPVNLSTPDKDKLEKELVKLKTLNKEISEKQMQLITLTQVSDNISQGLAMESGTAVKGQVNEMKAKITKLAEDVRQRLNAVSDTILARQEFQGKMEDFENWMDQLHTGVGQVDEVTLAQVDSALQSVHALLQEHSEKQPAFNAIYDEVRKLNCSSPEEASSVNESYSELVTKYQDLEDTLQEKKRALEKWSDLLSWYADTSGQLNHIKYQLESQKLQPEDVERVAEELEVINLKTASWTDVASIIDGVSQQSGTYIRDTATGKRVTAVALLHELETKVNVLQAQLSNKREVLEEVGARWAQFQRVQENLSEGLLSMQGTLHETVMEVDTCGKLEQAGKIIAGLLEEHKLKQGAKDELHRLGKLLMEEDQSNMSEVQTILASLDANWEKVSEMLKEQKVKSLEMSTAWKHFCDAKEKVYSGITDAEKLYELVQEVP-SDLTEAALSHDKTKKALELLKKAKLSLDVMDNKAQILLKQAEPISGFNSAVIGEELTEAHNAWQNAYDTLTKKVQVLEAQLIVWKQIDESKNELVQWLGETSDALLNASQDLSDVESGQSKLNRYKDELPAFYNLKTSLISKTAQLVKLNDGKQIPTLESLNKLLEDEFAHVKSIADKLEDITCAVGEQERSVRDDMKNASDTITKIREAVIACDDLTGENSKILERLKNCQALKNELQNFSSNLELLKKKIEEMKSSFPAFGDSGLSKELSSLQIRYDGVSSHANKTESTLLAFLNKYHMEKFGALQRGVAAHKEKVAWCLPEAGSDRYNLEVKVSSLQDVEVGLMDCETKKTDLDVSLDLLQNVETPEKIKELQ-----LERDK--LVTELESLKNSYLNTKQLLEHNISLWQKYELMSENVASWLKETEGKVRTESVIQLNLSTIDDKIKEMESFQKQVKDFEPEIKDISILGEEIMKENPESRVGHYVGHLVSRYHGVVKFAAMYMERLTSLNKTK 2913          

BLAST of PREDICTED: nesprin-1-like vs. nr
Match: gi|1339073114|ref|XP_023723815.1| (nesprin-1 isoform X4 [Cryptotermes secundus])

HSP 1 Score: 1768.82 bits (4580), Expect = 0.000e+0
Identity = 977/2665 (36.66%), Postives = 1586/2665 (59.51%), Query Frame = 0

Query:  578 SRPNSALANPESQEWYDKYQLKAFPHDSQFGEQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERL-RQLKSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDDIPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAVGEPEDLQIELAKHKPFLAKTVNMQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVP-QHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGK-AKGKLNQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFE---EEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWI-EAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKV--EINAKN-------KILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLEQTLDN-VTSLDGLEIALAELDSLAQQCQSKKNDGANDEEMAAKSKTYV--IQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQMVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKANVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKCLDRDKKEVMDEIAKLK---IDISELLLS----WRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNK 3216
            SRP S      S EWY +Y+ ++F + +   E   ++  YDSHI +I+DEQERVQKKTFVNWINSYL+KR+PP++I+DLID L+DGTRLLALLEVL GERLP E+GR L+RPH+LSN NTAL++L+SK+IKLVNIN++D+VDGRP VVLGLIWTIILYFQIEENTR LESL   +G  A      G   SE   G     +K    E+ + GAR+A+LHW  + + +  G+ V DFG SW+DG AFLAII++I+   ++  +MRQ SN+TRLETAF VAE +LG+ RLLDPEDVDVP PDEKS+MTYVAQFLHKYPE   A GS   G+++++Y EL +WL  K  + E +Q T   L M Y +++  K E   K +++ +L R ++S   + IT E+W E++  W K+ETQ+ +W WLLD+ LPG+ GQ+GEWL + E LI SDDIP ++NEE A+++++K+E+HK FFADL A+Q  F+ A  +   V+ +P +QL+S+ +R   IGP+A  R VRLKFLEHKCC++AFL LTE+KL+ WT+KYG+EE+VQQ++ QY+ FVS+N+IFQEF KA+++ Q V  EY+++G ID  ES SIDRF+ E   RWKN S ELRCVQS+LEEV+ YW+RWN   +  + ++  A+  L   E+ + E+F D + WK+++ LL DTV+FL+AT +  V  +LRE+++ +S  WE LF+  + ++ +GDI R ++ Y+ G+E+L QWLR AES+L+++Q   +  IK   E L +L +E+ G+EELFK++S+KFQ ++ +L  D+++ +M +LKK+KE LV +R++IP ++ LFH +L Q E+ + G+ E+ QW D+ ++L+ +       E +Q +L KHK F ++T+  +S+L+SK  VF+ ++K+ +  EG+D  D+  RM  +N RF    +  QQ+E  +  G++ W  F + ++ I  WL++A+ L   ++I++ Q V+ H++ F    E  + + +  AQ L   L       V    G ++A+W  V  +AP H ++ EFRL E   N+ +KEIE ++  E QAF K E++  I+  + ++F    ++ +    L  M++I   +    P D +L +       QW+    +I+ + ++L+QIP  W+ Y++KF +MV WMD VD +L  + K       F+ E+  FQ +C +VD +R+DMKWLVQ LDSLVS  +D   L+EQK+LE LI RYK +IP IE+TM K D++ K Y ++ E   V  LL  ++  S+    P T + V  ++ ++E  + QLD  R  ++SLLQ+G DL++D  AP F+K EV  LE+ WN+ Y + VE+ K      KVW  YKE K  +L+L++ +E EL +  P  HD ++V   L+ K+++   + KATE++L K+R+L   LST   PE++  L  E+  +E++L   LE  +E++ +L +   +W  F   L EL+ W  + A   L Q+   +++P++RVK +  LQ  ++ K+  L+ L  +A  L E          LK EV  ++  V  L+  V  +  ++ +DL  W   +  L  +KPW+E+AE++V +G+P+  +LQ+A + L+  + FE   EE   K   L   A     ++C     D+VDA+ SR   V     QW  RLE LV TW+ F+N   +ID WI E +    +   NL     D L  EL +LK LN  +++KQ++L+ L++  D ++  ++ ++   VK +V  +K +++ L++  R +++ + + I   QEFQ  + D  +W+  L++ + +++EV    +DS+L++VH+L QEH++ Q     +  E+   N         ++ S   +  KY DL D L+ KK++L++WS   +W+A++   LNH++  L++     + +E    EL+ +  +  S  +  +  + ++ +S TY+     GK  + + L+ ++  ++  L+  +  K + L  +  +W  F+ V++ L++ L  +   + +  +   T   L+ A   +A L  +   K   KD+  RLG+ L+  D   +S VQ  L+   + W+KV+ +LKEQ+ KS  +   W+     K+ V + + +  +  E ++  P     + A   DK +K L+ LKK +   +V   K   L+++ + +  F+ A +  ++ E    +     TL   +  LE+++++W Q ++  ++L  W+ +T D    A+ +++D+E+ + KL++Y ++LP+  + K ++ +K A LV+ N  K I  L++  + ++  FA+V+     L++    + +   ++R  +K   D +  +RE  + C+D TG +S+ILERL     L+ E  +F  ++  ++ +I+++   F A   + L+KELSSL  RYD VSS   KT   L   L K+++EK  +  + +A+ KEK+ WC P+   D+++LE K+ +L ++   L   +   + L    DLL  V   +K  E++     L+RDK  ++ E+  LK   ++  +LL      W+  +L+S+ +A WLK +E   R E      L T  +   ++E  + ++   +  +++++   + +M  +P++RV      L +RY          ++RL  L   K
Sbjct:  268 SRPGS---TSSSIEWYSEYRTQSFHNMAAKLEYIRSRSQYDSHIAEIKDEQERVQKKTFVNWINSYLSKRVPPLRIDDLIDDLKDGTRLLALLEVLSGERLPVERGRNLKRPHFLSNANTALQFLQSKKIKLVNINSSDLVDGRPPVVLGLIWTIILYFQIEENTRALESLDHAFGGSASSLESLGARSSE---GVAVEGKKKEPNERRRQGARRAMLHWVTSTLPKDHGIEVRDFGGSWQDGFAFLAIIDAIKSNLINFPAMRQASNKTRLETAFNVAESELGIARLLDPEDVDVPQPDEKSIMTYVAQFLHKYPEPRAADGSSTLGAIEAEYNELISWLLKKTQYLEHLQQTNS-LSMVYSDYKTFKGEFDEKAKVFGKLKRVIESQSMVTITVESWREIERLWTKLETQLHNWLWLLDSGLPGDLGQVGEWLGRAEALIASDDIPTIMNEETASIISRKLEEHKVFFADLPAIQANFQRAKLSHDAVD-VPSEQLESLTERFEMIGPRAAQRRVRLKFLEHKCCLIAFLHLTETKLRGWTVKYGQEEKVQQLLEQYRNFVSRNRIFQEFNKAYIDMQQVVEEYRREGNIDQRESVSIDRFMHETGERWKNVSMELRCVQSMLEEVVAYWRRWNTLAEEFESWLDRAYGLLDLPEEDKMEYFQDVSVWKDKFQLLGDTVSFLIATCEDQVAHELRERYLRISARWEDLFQHVKQYMHAGDILRHRKDYRAGVERLQQWLRNAESILSSSQLSSTEKIKIYGEQLQQLQNEIEGIEELFKNVSKKFQAMIQDLSRDEVDRIMNTLKKEKEALVRVRALIPMQLHLFHQILVQQESLEAGQNEIGQWLDEAEALIASHSLAAGKEPVQAQLDKHKTFFSRTLYYKSMLESKNKVFRSIVKSVDHNEGIDTGDLKLRMAQLNERFIRVTQLAQQWELKLQEGIRCWHNFRENERIITEWLQKAEKLIAERHIDTKQTVESHKNFFEKVNEKWIQDLVNSAQDLRNCLPYEQHPPVDAAVGRLQAKWKEVLSFAPLHLMRLEFRLDEAAFNQYLKEIEKEIHSEQQAFNKHEDVEAILTRNREFFVECKVVVEIERCLENMSKISNNFNQWDPHDSSLREAHGRAVAQWEGASRKIETISQQLQQIPTLWKNYQQKFLEMVHWMDKVDINLQNIVKEVNTLEEFDKERAVFQSICCEVDAKREDMKWLVQTLDSLVSHCSDKESLAEQKKLEQLIARYKVLIPTIELTMVKTDVHYKCYTYRKEVREVVGLLQKVKETSMSGPQPETLDSVNVMIKQQETAVTQLDHQRSNIMSLLQRGKDLVKDTHAPSFIKSEVSNLETGWNEAYGQTVEKLKKLKGTQKVWTNYKEQKDEILKLLEQAEGELKRVAPGAHDSQKVAADLQAKQDMSVALRKATEEMLRKLRDLCSTLSTMTAPERKPLLQKEVTEIERRLHVTLETVEERVIYLEQFSARWIRFQAQLDELRHWTHQSAPVLLEQLQATELTPEERVKKSQALQAQLEDKINVLDVLSGEAKELLEEDGENVEAAQLKAEVMALRDSVLGLHQGVLMQISSVGQDLAHWQDYKTGLQEVKPWIEEAEVRVNIGIPKPMSLQEAQHLLSSTRTFEKQCEEYLAK---LQGLAALSQQITCRTNAPDEVDAVHSRWAGVHDVAVQWGTRLEKLVGTWEEFENEAKKIDTWIQEGQKAIFETPVNLSTPDKDKLEKELVKLKTLNKEISEKQMQLITLTQVSDNISQGLAMESGTAVKGQVNEMKAKITKLAEDVRQRLNAVSDTILARQEFQGKMEDFENWMDQLHTGVGQVDEVTLAQVDSALQSVHALLQEHSEKQPAFNAIYDEVRKLNCSSPEEASSVNESYSELVTKYQDLEDTLQEKKRALEKWSDLLSWYADTSGQLNHIKYQLESQKLQPEDVERVAEELEVINLKTASWTDVASIIDGVSQQSGTYIRDTATGKRVTAVALLHELETKVNVLQAQLSNKREVLEEVGARWAQFQRVQENLSEGLLSMQGTLHETVMEVDTCGKLEQAGKIIAGLLEEHKLKQGAKDELHRLGKLLMEEDQSNMSEVQTILASLDANWEKVSEMLKEQKVKSLEMSTAWKHFCDAKEKVYSGITDAEKLYELVQEVP-SDLTEAALSHDKTKKALELLKKAKLSLDVMDNKAQILLKQAEPISGFNSAVIGEELTEAHNAWQNAYDTLTKKVQVLEAQLIVWKQIDESKNELVQWLGETSDALLNASQDLSDVESGQSKLNRYKDELPAFYNLKTSLISKTAQLVKLNDGKQIPTLESLNKLLEDEFAHVKSIADKLEDITCAVGEQERSVRDDMKNASDTITKIREAVIACDDLTGENSKILERLKNCQALKNELQNFSSNLELLKKKIEEMKSSFPAFGDSGLSKELSSLQIRYDGVSSHANKTESTLLAFLNKYHMEKFGALQRGVAAHKEKVAWCLPEAGSDRYNLEVKVSSLQDVEVGLMDCETKKTDLDVSLDLLQNVETPEKIKELQ-----LERDK--LVTELESLKNSYLNTKQLLEHNISLWQKYELMSENVASWLKETEGKVRTESVIQLNLSTIDDKIKEMESFQKQVKDFEPEIKDISILGEEIMKENPESRVGHYVGHLVSRYHGVVKFAAMYMERLTSLNKTK 2913          

BLAST of PREDICTED: nesprin-1-like vs. nr
Match: gi|1339073112|ref|XP_023723814.1| (uncharacterized protein LOC111873382 isoform X3 [Cryptotermes secundus])

HSP 1 Score: 1768.44 bits (4579), Expect = 0.000e+0
Identity = 977/2665 (36.66%), Postives = 1587/2665 (59.55%), Query Frame = 0

Query:  578 SRPNSALANPESQEWYDKYQLKAFPHDSQFGEQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERL-RQLKSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDDIPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAVGEPEDLQIELAKHKPFLAKTVNMQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFELNKAVP-QHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGK-AKGKLNQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFE---EEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWI-EAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKV--EINAKN-------KILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLEQTLDN-VTSLDGLEIALAELDSLAQQCQSKKNDGANDEEMAAKSKTYV--IQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQMVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKANVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKCLDRDKKEVMDEIAKLK---IDISELLLS----WRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNK 3216
            SRP S  +   S EWY +Y+ ++F + +   E   ++  YDSHI +I+DEQERVQKKTFVNWINSYL+KR+PP++I+DLID L+DGTRLLALLEVL GERLP E+GR L+RPH+LSN NTAL++L+SK+IKLVNIN++D+VDGRP VVLGLIWTIILYFQIEENTR LESL   +G  A      G   SE   G     +K    E+ + GAR+A+LHW  + + +  G+ V DFG SW+DG AFLAII++I+   ++  +MRQ SN+TRLETAF VAE +LG+ RLLDPEDVDVP PDEKS+MTYVAQFLHKYPE   A GS   G+++++Y EL +WL  K  + E +Q T   L M Y +++  K E   K +++ +L R ++S   + IT E+W E++  W K+ETQ+ +W WLLD+ LPG+ GQ+GEWL + E LI SDDIP ++NEE A+++++K+E+HK FFADL A+Q  F+ A  +   V+ +P +QL+S+ +R   IGP+A  R VRLKFLEHKCC++AFL LTE+KL+ WT+KYG+EE+VQQ++ QY+ FVS+N+IFQEF KA+++ Q V  EY+++G ID  ES SIDRF+ E   RWKN S ELRCVQS+LEEV+ YW+RWN   +  + ++  A+  L   E+ + E+F D + WK+++ LL DTV+FL+AT +  V  +LRE+++ +S  WE LF+  + ++ +GDI R ++ Y+ G+E+L QWLR AES+L+++Q   +  IK   E L +L +E+ G+EELFK++S+KFQ ++ +L  D+++ +M +LKK+KE LV +R++IP ++ LFH +L Q E+ + G+ E+ QW D+ ++L+ +       E +Q +L KHK F ++T+  +S+L+SK  VF+ ++K+ +  EG+D  D+  RM  +N RF    +  QQ+E  +  G++ W  F + ++ I  WL++A+ L   ++I++ Q V+ H++ F    E  + + +  AQ L   L       V    G ++A+W  V  +AP H ++ EFRL E   N+ +KEIE ++  E QAF K E++  I+  + ++F    ++ +    L  M++I   +    P D +L +       QW+    +I+ + ++L+QIP  W+ Y++KF +MV WMD VD +L  + K       F+ E+  FQ +C +VD +R+DMKWLVQ LDSLVS  +D   L+EQK+LE LI RYK +IP IE+TM K D++ K Y ++ E   V  LL  ++  S+    P T + V  ++ ++E  + QLD  R  ++SLLQ+G DL++D  AP F+K EV  LE+ WN+ Y + VE+ K      KVW  YKE K  +L+L++ +E EL +  P  HD ++V   L+ K+++   + KATE++L K+R+L   LST   PE++  L  E+  +E++L   LE  +E++ +L +   +W  F   L EL+ W  + A   L Q+   +++P++RVK +  LQ  ++ K+  L+ L  +A  L E          LK EV  ++  V  L+  V  +  ++ +DL  W   +  L  +KPW+E+AE++V +G+P+  +LQ+A + L+  + FE   EE   K   L   A     ++C     D+VDA+ SR   V     QW  RLE LV TW+ F+N   +ID WI E +    +   NL     D L  EL +LK LN  +++KQ++L+ L++  D ++  ++ ++   VK +V  +K +++ L++  R +++ + + I   QEFQ  + D  +W+  L++ + +++EV    +DS+L++VH+L QEH++ Q     +  E+   N         ++ S   +  KY DL D L+ KK++L++WS   +W+A++   LNH++  L++     + +E    EL+ +  +  S  +  +  + ++ +S TY+     GK  + + L+ ++  ++  L+  +  K + L  +  +W  F+ V++ L++ L  +   + +  +   T   L+ A   +A L  +   K   KD+  RLG+ L+  D   +S VQ  L+   + W+KV+ +LKEQ+ KS  +   W+     K+ V + + +  +  E ++  P     + A   DK +K L+ LKK +   +V   K   L+++ + +  F+ A +  ++ E    +     TL   +  LE+++++W Q ++  ++L  W+ +T D    A+ +++D+E+ + KL++Y ++LP+  + K ++ +K A LV+ N  K I  L++  + ++  FA+V+     L++    + +   ++R  +K   D +  +RE  + C+D TG +S+ILERL     L+ E  +F  ++  ++ +I+++   F A   + L+KELSSL  RYD VSS   KT   L   L K+++EK  +  + +A+ KEK+ WC P+   D+++LE K+ +L ++   L   +   + L    DLL  V   +K  E++     L+RDK  ++ E+  LK   ++  +LL      W+  +L+S+ +A WLK +E   R E      L T  +   ++E  + ++   +  +++++   + +M  +P++RV      L +RY          ++RL  L   K
Sbjct:  268 SRPGSTSS---SIEWYSEYRTQSFHNMAAKLEYIRSRSQYDSHIAEIKDEQERVQKKTFVNWINSYLSKRVPPLRIDDLIDDLKDGTRLLALLEVLSGERLPVERGRNLKRPHFLSNANTALQFLQSKKIKLVNINSSDLVDGRPPVVLGLIWTIILYFQIEENTRALESLDHAFGGSASSLESLGARSSE---GVAVEGKKKEPNERRRQGARRAMLHWVTSTLPKDHGIEVRDFGGSWQDGFAFLAIIDAIKSNLINFPAMRQASNKTRLETAFNVAESELGIARLLDPEDVDVPQPDEKSIMTYVAQFLHKYPEPRAADGSSTLGAIEAEYNELISWLLKKTQYLEHLQQTNS-LSMVYSDYKTFKGEFDEKAKVFGKLKRVIESQSMVTITVESWREIERLWTKLETQLHNWLWLLDSGLPGDLGQVGEWLGRAEALIASDDIPTIMNEETASIISRKLEEHKVFFADLPAIQANFQRAKLSHDAVD-VPSEQLESLTERFEMIGPRAAQRRVRLKFLEHKCCLIAFLHLTETKLRGWTVKYGQEEKVQQLLEQYRNFVSRNRIFQEFNKAYIDMQQVVEEYRREGNIDQRESVSIDRFMHETGERWKNVSMELRCVQSMLEEVVAYWRRWNTLAEEFESWLDRAYGLLDLPEEDKMEYFQDVSVWKDKFQLLGDTVSFLIATCEDQVAHELRERYLRISARWEDLFQHVKQYMHAGDILRHRKDYRAGVERLQQWLRNAESILSSSQLSSTEKIKIYGEQLQQLQNEIEGIEELFKNVSKKFQAMIQDLSRDEVDRIMNTLKKEKEALVRVRALIPMQLHLFHQILVQQESLEAGQNEIGQWLDEAEALIASHSLAAGKEPVQAQLDKHKTFFSRTLYYKSMLESKNKVFRSIVKSVDHNEGIDTGDLKLRMAQLNERFIRVTQLAQQWELKLQEGIRCWHNFRENERIITEWLQKAEKLIAERHIDTKQTVESHKNFFEKVNEKWIQDLVNSAQDLRNCLPYEQHPPVDAAVGRLQAKWKEVLSFAPLHLMRLEFRLDEAAFNQYLKEIEKEIHSEQQAFNKHEDVEAILTRNREFFVECKVVVEIERCLENMSKISNNFNQWDPHDSSLREAHGRAVAQWEGASRKIETISQQLQQIPTLWKNYQQKFLEMVHWMDKVDINLQNIVKEVNTLEEFDKERAVFQSICCEVDAKREDMKWLVQTLDSLVSHCSDKESLAEQKKLEQLIARYKVLIPTIELTMVKTDVHYKCYTYRKEVREVVGLLQKVKETSMSGPQPETLDSVNVMIKQQETAVTQLDHQRSNIMSLLQRGKDLVKDTHAPSFIKSEVSNLETGWNEAYGQTVEKLKKLKGTQKVWTNYKEQKDEILKLLEQAEGELKRVAPGAHDSQKVAADLQAKQDMSVALRKATEEMLRKLRDLCSTLSTMTAPERKPLLQKEVTEIERRLHVTLETVEERVIYLEQFSARWIRFQAQLDELRHWTHQSAPVLLEQLQATELTPEERVKKSQALQAQLEDKINVLDVLSGEAKELLEEDGENVEAAQLKAEVMALRDSVLGLHQGVLMQISSVGQDLAHWQDYKTGLQEVKPWIEEAEVRVNIGIPKPMSLQEAQHLLSSTRTFEKQCEEYLAK---LQGLAALSQQITCRTNAPDEVDAVHSRWAGVHDVAVQWGTRLEKLVGTWEEFENEAKKIDTWIQEGQKAIFETPVNLSTPDKDKLEKELVKLKTLNKEISEKQMQLITLTQVSDNISQGLAMESGTAVKGQVNEMKAKITKLAEDVRQRLNAVSDTILARQEFQGKMEDFENWMDQLHTGVGQVDEVTLAQVDSALQSVHALLQEHSEKQPAFNAIYDEVRKLNCSSPEEASSVNESYSELVTKYQDLEDTLQEKKRALEKWSDLLSWYADTSGQLNHIKYQLESQKLQPEDVERVAEELEVINLKTASWTDVASIIDGVSQQSGTYIRDTATGKRVTAVALLHELETKVNVLQAQLSNKREVLEEVGARWAQFQRVQENLSEGLLSMQGTLHETVMEVDTCGKLEQAGKIIAGLLEEHKLKQGAKDELHRLGKLLMEEDQSNMSEVQTILASLDANWEKVSEMLKEQKVKSLEMSTAWKHFCDAKEKVYSGITDAEKLYELVQEVP-SDLTEAALSHDKTKKALELLKKAKLSLDVMDNKAQILLKQAEPISGFNSAVIGEELTEAHNAWQNAYDTLTKKVQVLEAQLIVWKQIDESKNELVQWLGETSDALLNASQDLSDVESGQSKLNRYKDELPAFYNLKTSLISKTAQLVKLNDGKQIPTLESLNKLLEDEFAHVKSIADKLEDITCAVGEQERSVRDDMKNASDTITKIREAVIACDDLTGENSKILERLKNCQALKNELQNFSSNLELLKKKIEEMKSSFPAFGDSGLSKELSSLQIRYDGVSSHANKTESTLLAFLNKYHMEKFGALQRGVAAHKEKVAWCLPEAGSDRYNLEVKVSSLQDVEVGLMDCETKKTDLDVSLDLLQNVETPEKIKELQ-----LERDK--LVTELESLKNSYLNTKQLLEHNISLWQKYELMSENVASWLKETEGKVRTESVIQLNLSTIDDKIKEMESFQKQVKDFEPEIKDISILGEEIMKENPESRVGHYVGHLVSRYHGVVKFAAMYMERLTSLNKTK 2913          

BLAST of PREDICTED: nesprin-1-like vs. nr
Match: gi|1000755574|ref|XP_015600724.1| (PREDICTED: uncharacterized protein LOC107270320 isoform X13 [Cephus cinctus])

HSP 1 Score: 1701.8 bits (4406), Expect = 0.000e+0
Identity = 963/2697 (35.71%), Postives = 1547/2697 (57.36%), Query Frame = 0

Query:  569 RAPSLEQIPSRPNSALANPESQ------EWYDKYQLKAFPHDSQFGEQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERLRQL-KSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDD-IPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAVGEPEDLQIELAKHKPFLAKTVNMQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFEL-NKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKL-NQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKVEINAKN----------KILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLE-QTLDNVTSLDGLEIALAELDSLAQQCQS-KKNDGANDEEMAAKSKTYVIQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQ---MVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKANVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKC--------LDRDKKEV-MDEI----AKLKIDISELLLSWRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNKGAQMDAINEYE 3227
            R PS E +  R  S+  NP S       EWY +Y+ ++F + +   E + ++  YD+HI QIRDEQERVQKKTFVNWINSYL KR+PP++IEDLI+ L+DGTRLLALLEVL GE+LP E+GR L+RPH+LSN NTAL++L+SK+IKLVNIN++D+VDGRP VVLGLIWTIILYFQIEENTR LE LGQ +GS         + ES   +G+    R+    EKWK GARK LL W    + +  G+ V DFG+SWRDGNAFLAII++I+   V++ +MR+ SN+ RLETAF VAE +LG+ RLLDPEDVDVP PDEKS+MTYVAQFLHKYPE   A G D F ++  +Y  L  WL  ++   +Q+  T    P +Y E+   K E  +K  IY +L+ L  +   + IT ++W +++  W+K+E+ +  W W LD+ LPGEFG++G WL Q E L+ SD+ IP  + EE A++++ K+EDHK FF DL A+ T+      NSP   +IP +QLQ+M  RL  +  +A  R +RLKFLEHKCC++AFL L E+KLK W++KYG EE+V Q++ QY+ FVS+N+IFQEFQKA+++ Q V  EYK++GE+D EE+ +IDRF+ +   +WKN S +LRCVQS+LEEV+ YW+RW       + ++  A  AL+  E+ + EFF D + WK+++  L DTV+FL+ATSD  V   L+ ++  +S  WE+LF +A+ ++ +GD+ R ++ Y+ G+E L  WLR AES+L+TTQ   +  IK   E L  LH+EV G+E+LFK++S+KFQ L+ +L  D+++ MM +LKK+KE LV +R++IP ++ L+H LL Q E+ + G++E+  W DD + L+  +   G  +    +L +HK F ++T+  +S+L+SK  VF  ++K+ +    +D ++    +  +N RF    ++ Q  E  +   ++ W KF + ++ I  WL  A+ L N K+I++ Q V+ H++ F    E  + + +   Q L   + +  +A + E    ++  W  V  +AP H ++ EFRL E    + +KEIE ++  E QA  K++ +  I+Q + D+F    I+ +    L  + +I   Y  L P D +L +     ++QW+   +RI+ + ++L Q+PEQW  Y +KF++MVRWMD VD +L  + +       FE E+  FQ++CR+ D++R++MKWLVQ LDSL S R D A ++EQ +LE LITRYK++IP IE+TM K DIYSKSY ++ E   V  LL  +++++         E +   +  +E  L QL+  R  ++S+LQ+G DL++D  AP F+  EV+ LE  WND Y + +E  K      K+W+ Y++ K  +L+LI+ ++ EL N     HD  +V   L+ K+ L   + KA E++L K+R++   LS A  P+++  L  E+  +E+KL   LE   E++ +L + + +WT+F   LGEL+ W  ++  +L   I +   SP++RV+ T  LQ+ +  K+  L+ LE ++  L  +         L+ E++ ++  V++LN ++  +     K+L  W   +  +  +KPW+E+AE K A    + T L  A+  L   K FE++       + + +     ++      D+VDA+ +R  AV     Q   +L+ LV +WK F+    E + W++          N+       L  EL +LK  N  ++ +Q +L+ L++  D ++  ++ + A  +K +V  LK +++ L+D  R +++ + + +   QEFQ  I D  +W+  L + I E++EV  +++D++L++VH+  QEH++ Q   A +    K+          K L+    ++ KKY  L D L  KKK L++W+   +WH E+ + LNH + Q   +  ++  LE   +EL S+  +  S K+     D  M  + +    +  KP +   LV D+  + V L+  + AK DKL +L  +WDNFK ++ +L + +      +Q+I     + + L  A   ++EL  +   +   KD   R G  LV  D + V+ +Q  LS A + W+KV  LL+EQQ K   + A W+Q L  K+ +   + E     + ++  P     D+ Q    +DK +K  D LKK +   E    K  QLI+E   +PNF    + +D+ +V+K+Y      +       E+++++W Q ++   +L+ W+ DT D    A  ++ D E  + KL++Y  +LP+    +  ++ K   L++ N+   I  L +    +D  F  V+     L+      ++    IR ++K   D ++ +RE  + C+D TG +++IL R+    +L+ E +    ++  +E +I  +S ++ A   + L KEL +L  R D V+S   K +  L   L K Y EK  +  + +A+ KEK  WC+P+Q  D+++LE KM +L ++                +AD   + AD D   ++  TV+          DRDK    +D +    AK+K  +   +  W+  +L+S+ +  WLK +E   R E +    L   +   +++ QL+  +    + L++L+   + ++ +SP++RV Q    L  RY+A    + + L+RL++L+ ++      + E E
Sbjct:  171 RFPSEESLSQRRGSSPQNPGSSDERTPAEWYAEYRNQSFQNVTARMEYNRSRSEYDAHIAQIRDEQERVQKKTFVNWINSYLLKRVPPLRIEDLIEDLKDGTRLLALLEVLSGEKLPVERGRNLKRPHFLSNANTALQFLQSKKIKLVNINSSDLVDGRPPVVLGLIWTIILYFQIEENTRALEYLGQTWGS-------QSSLESLGTQGSAASERRRQSSEKWKQGARKTLLQWVTNALPKDAGIHVRDFGESWRDGNAFLAIIDAIKANLVNIAAMREASNKARLETAFQVAETELGIARLLDPEDVDVPQPDEKSIMTYVAQFLHKYPEPRSA-GPDTFAAVQREYDTLLEWLNDRVRHLDQLNRTNA-FPPSYPEYMLFKTETDDKGVIYNKLQGLVDTPSMISITRDSWKQIETLWKKMESLMLRWLWSLDSTLPGEFGEVGRWLAQAESLLMSDNNIPEEMTEETASIISNKLEDHKKFFLDLPAM-TELLQRAKNSPLAPKIPPEQLQNMTSRLDSLPSRAAKRRIRLKFLEHKCCLIAFLFLVETKLKGWSVKYGTEEKVHQMLEQYRNFVSRNRIFQEFQKAYMDMQQVVDEYKREGEVDHEENINIDRFMRQTGEKWKNVSMDLRCVQSMLEEVVAYWRRWTTVSDEFESWLQRAEPALELPEEEKMEFFQDISVWKDKHQQLSDTVSFLIATSDDQVALQLKNRYTNLSARWEKLFPEAKQYMHAGDLLRNRKDYRAGVETLQSWLRNAESILSTTQLSSTEQIKAYGEQLKVLHNEVEGIEDLFKNISKKFQTLIQDLSRDEVDKMMNTLKKEKEALVRVRALIPMQLHLYHQLLVQQESLEAGQKEISNWLDDAERLLAGINLSGGRDSALAQLDRHKAFFSRTLYYKSMLESKNKVFSSIVKSVDSHANVDTAEGGAALRELNERFAQVSQSAQLAEQRLQEAVRCWTKFRECERQISEWLSAAETLINDKHIDNRQSVEYHKNFFGKVNEKWIQDLVNTGQDLRNAIEQ--EAPIVEAVDFLQKRWKEVLTFAPLHLMRLEFRLDEATFLQYLKEIEVEINSEQQALIKNDEVGNILQRNKDFFVNRGIVLEVERCLQTLKKISTAYTQLKPSDSSLNEATQKAEHQWEDSAQRIEQLREQLRQVPEQWAAYREKFAEMVRWMDHVDTTLRTILQEVNTLEEFEQERSIFQKICREADSKREEMKWLVQTLDSLTSNRTDHAAVTEQNQLEQLITRYKNLIPTIEITMTKTDIYSKSYTYRKEVREVCTLLKKVRDQTSIQEASENPETLTTALTHQETRLSQLEQQRANIVSMLQRGKDLLKDQHAPTFVSTEVQHLEISWNDTYGQSIEALKVLRGSQKLWNSYQQQKDEILKLIEQADKELKNIGTGYHDSAQVSADLQSKQELSISLRKAAEEMLRKLRDIHANLSEATVPQKRPLLEKEVTDIEKKLHITLETVQERVVYLQQFNARWTKFQARLGELQTWTQQSAPQLIANIQETSTSPEERVRKTEILQKQIHEKITILKVLEEESRKLITDDSENVEAKALRSEIKDLQNAVQSLNASIVTQREIATKNLTTWKEYEVGIQQLKPWIEQAESKAASIGSKPTTLDQAIKMLETAKAFEKQCEEHLPRVQELSVISQQIAGRTAAPDEVDAVHTRWNAVHDVAIQTTTKLDKLVSSWKTFETEAKEFNVWLQDSEKTALVEPNVQTPETGKLENELARLKEFNKAISDRQAQLISLTQVSDHISHGLALEGATNLKGRVSELKTRVNKLADIVRHQINRVSDALLARQEFQMKITDFENWMSRLRTNIAEISEVNVDNVDTNLQSVHAYLQEHSEKQPCFASIYEEVKHLSAQGSPEEAKALNEIYTSLAKKYKALEDDLCQKKKGLEKWAELLSWHNETSAQLNHSKYQVEAHKPTIPDLERLSSELQSVHTKISSWKEQISIIDSSMGVRVRD---KQEKPLTASALVNDLEAKAVTLQTELTAKRDKLENLSARWDNFKKLQTKLTEEIVHTQTTLQEITYSVDSCEKLAPAVEKISELIEEHRHREPDKDTLHREGATLVKEDQRAVTNIQVVLSSADANWEKVNELLREQQSKYSEMNADWKQFLEAKEKLKKFVKESKALCQSVKEVP----NDITQANIALDKHKKAADILKKGKQFLEKMDAKAQQLIKEASLMPNFKSELIESDLADVRKEYQDAYSNIVERSQTYETQVIIWKQIDEAKYELTRWLSDTNDALTTACEHLPDAENGQTKLARYREELPAHQLLRQGISTKTEQLIKLNNNAEIPTLNSLNRLLDDQFKVVKASADKLESLTSSFNEKEKGIRQELKKFSDLISKIREDIIKCDDLTGENTKILGRINKCQELKNELEKCNYNLSDVEEKITSMSSEYPAITKSSLPKELQALQLRRDGVASHADKVSATLVAFLTKLYHEKFGALQRMVATHKEKAAWCEPEQSSDRYNLEVKMASLVDVE-------------TGIADCEARKADTDNSLKLLQTVESSETMEGLRADRDKVAADLDSLKTSYAKIKSVLEHNIALWQKYELMSENVVCWLKETENKIRIESSALLTLPDIENKINEIGQLQKAVSDYRRELDDLSALGEDIIKVSPESRVGQYIGHLNTRYQAVLKFLASHLERLEELSKSRDQYKSNVKELE 2835          

HSP 2 Score: 70.4774 bits (171), Expect = 9.850e-8
Identity = 185/944 (19.60%), Postives = 382/944 (40.47%), Query Frame = 0

Query: 2050 FHKNLGELKDWVGKAKGKLNQITKLDVSPDDRVKMTLELQQ--DVKCKMKQLEKLETDAHN---LFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAM---GLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNL-GDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEV--PFNHIDSSLENVHSLAQE---HADN----QSRIAKVEINAKNKILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFC-TWHAESD---SALNHLEQTLDNVT---SLDGLEIAL--------AELDSLAQQCQSKKN---DGANDEEMAAKSKTYVIQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKV--QKINVREST----IDCLKT----AASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDE----VHQAMEDL-EHTPIRSKKDVAQMVDKCRKGL-DTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKA---NVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQ 2938
            +H+  G L+  V   K K         S  DR  + +++    DV+  +   E  + D  N   L + V  + T+  L+ + + V  D+++L  +       +  ++ +W   +    N+  WL++ E K+ +    L    ++++ +NE+ QL++   +   +   L    +    +S    +   +  L +R QAV   +   + RLE+L K+   + +   E++ WI+     +     + G   I      L +LKA  +   + Q  L   ++  + +   ++ D  +V++ ++ +L+ Q+  L+D +      I  ++     F+D    V  W+ D  +++ +  ++         +L +  ++AQ+   H +     Q R+  +  +   ++L   I A  K   D+ D +   +K +    ++  T+    D   + +N     ++++T        +IAL        AE D + Q C  + N   +  +     A  K Y  Q         +  D L+R +  +       D+L+ L D+W  F+ V ++L  W++Q+  +V  Q +   E T    +  LKT     A+  AE +A  ++  +++ + E LG +               +S   +K+  + +  KE        +A +EQ +    +   + ++    +    +DL +H+ I     V Q   K  + L DT  K    FE       +L     T P+     +R  ++ ++  +    + L+     L+  ++ +A++    + L+ W++D +          + L+  + +L  +      +MS ++   +V  K   LV+         L   L+ + Q F N+  +   L E +E      +    + K   D L   REK + C D  G  SEI  RL  +  L+
Sbjct: 2611 YHEKFGALQRMVATHKEKAAWCEPEQSS--DRYNLEVKMASLVDVETGIADCEARKADTDNSLKLLQTVESSETMEGLRADRDKVAADLDSLKTSYAKIKSVLEHNIALWQKYELMSENVVCWLKETENKIRIESSALLTLPDIENKINEIGQLQKAVSDYRRELDDLSALGEDIIKVSPESRVGQYIGHLNTRYQAVLKFLASHLERLEELSKSRDQYKSNVKELEAWIQNAEKKLNVFREITGPKPITFYQSRLKELKAFAEEREKGQSILNRTAEAGEALYARITPDNREVIRAELRNLRNQVDALADRANVIYKRIESDMMHRSSFEDKYSQVKQWVIDAQAKLGDKQDLLASLQEKKLALHSYRTIAQDVNVHRNILQQLQDRLGTMSDDEATEMLGTVIEAYDKLSGDVEDRINIAEKHVANHEAYLQTFEKTRDWINTIVNEAAPIIEDLTIERDTAKAKIALIGNILQQKAEGDRVLQDCNQQLNIILEQTSITGHPALLKGYEEQKR-------IWDDFLKRCLGTR-------DRLNQLFDQWAEFEKVVERLEAWIKQMESQVKDQSLKSTEETKRAHLQKLKTLEEEIAAKGAEFNAAVEKSQTIEGESE-LGTQ---------------VSRLATKYQAIRNQAKE-------AVARYEQFVREHGVFNEKYNQFLKWITNVQDDLTKHSEIVGDLTVLQSRQKSIRDLGDTRTKENARFESVIDLGEKLY--AHTSPDGR-EIIRQQLRNLRTVWDGFSEDLQNATQKLDQCLMQFAEFSLSQEQLTTWLRDVERAMHQHTELKSSLQEKRAQLQNHKIMHQEIMSHQSLVESVCDKAQQLVDQTKDT---SLNVYLQSIKQLFHNIVAKSQDLLENLEDCVDKHNQFNVQCKNFTDWLNGEREKLLDCNDIAGERSEISRRLATLEALR 3509          

BLAST of PREDICTED: nesprin-1-like vs. nr
Match: gi|1000755566|ref|XP_015600720.1| (PREDICTED: uncharacterized protein LOC107270320 isoform X9 [Cephus cinctus])

HSP 1 Score: 1701.8 bits (4406), Expect = 0.000e+0
Identity = 963/2697 (35.71%), Postives = 1547/2697 (57.36%), Query Frame = 0

Query:  569 RAPSLEQIPSRPNSALANPESQ------EWYDKYQLKAFPHDSQFGEQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERLRQL-KSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDD-IPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAVGEPEDLQIELAKHKPFLAKTVNMQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFEL-NKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKL-NQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKVEINAKN----------KILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLE-QTLDNVTSLDGLEIALAELDSLAQQCQS-KKNDGANDEEMAAKSKTYVIQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQ---MVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKANVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKC--------LDRDKKEV-MDEI----AKLKIDISELLLSWRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNKGAQMDAINEYE 3227
            R PS E +  R  S+  NP S       EWY +Y+ ++F + +   E + ++  YD+HI QIRDEQERVQKKTFVNWINSYL KR+PP++IEDLI+ L+DGTRLLALLEVL GE+LP E+GR L+RPH+LSN NTAL++L+SK+IKLVNIN++D+VDGRP VVLGLIWTIILYFQIEENTR LE LGQ +GS         + ES   +G+    R+    EKWK GARK LL W    + +  G+ V DFG+SWRDGNAFLAII++I+   V++ +MR+ SN+ RLETAF VAE +LG+ RLLDPEDVDVP PDEKS+MTYVAQFLHKYPE   A G D F ++  +Y  L  WL  ++   +Q+  T    P +Y E+   K E  +K  IY +L+ L  +   + IT ++W +++  W+K+E+ +  W W LD+ LPGEFG++G WL Q E L+ SD+ IP  + EE A++++ K+EDHK FF DL A+ T+      NSP   +IP +QLQ+M  RL  +  +A  R +RLKFLEHKCC++AFL L E+KLK W++KYG EE+V Q++ QY+ FVS+N+IFQEFQKA+++ Q V  EYK++GE+D EE+ +IDRF+ +   +WKN S +LRCVQS+LEEV+ YW+RW       + ++  A  AL+  E+ + EFF D + WK+++  L DTV+FL+ATSD  V   L+ ++  +S  WE+LF +A+ ++ +GD+ R ++ Y+ G+E L  WLR AES+L+TTQ   +  IK   E L  LH+EV G+E+LFK++S+KFQ L+ +L  D+++ MM +LKK+KE LV +R++IP ++ L+H LL Q E+ + G++E+  W DD + L+  +   G  +    +L +HK F ++T+  +S+L+SK  VF  ++K+ +    +D ++    +  +N RF    ++ Q  E  +   ++ W KF + ++ I  WL  A+ L N K+I++ Q V+ H++ F    E  + + +   Q L   + +  +A + E    ++  W  V  +AP H ++ EFRL E    + +KEIE ++  E QA  K++ +  I+Q + D+F    I+ +    L  + +I   Y  L P D +L +     ++QW+   +RI+ + ++L Q+PEQW  Y +KF++MVRWMD VD +L  + +       FE E+  FQ++CR+ D++R++MKWLVQ LDSL S R D A ++EQ +LE LITRYK++IP IE+TM K DIYSKSY ++ E   V  LL  +++++         E +   +  +E  L QL+  R  ++S+LQ+G DL++D  AP F+  EV+ LE  WND Y + +E  K      K+W+ Y++ K  +L+LI+ ++ EL N     HD  +V   L+ K+ L   + KA E++L K+R++   LS A  P+++  L  E+  +E+KL   LE   E++ +L + + +WT+F   LGEL+ W  ++  +L   I +   SP++RV+ T  LQ+ +  K+  L+ LE ++  L  +         L+ E++ ++  V++LN ++  +     K+L  W   +  +  +KPW+E+AE K A    + T L  A+  L   K FE++       + + +     ++      D+VDA+ +R  AV     Q   +L+ LV +WK F+    E + W++          N+       L  EL +LK  N  ++ +Q +L+ L++  D ++  ++ + A  +K +V  LK +++ L+D  R +++ + + +   QEFQ  I D  +W+  L + I E++EV  +++D++L++VH+  QEH++ Q   A +    K+          K L+    ++ KKY  L D L  KKK L++W+   +WH E+ + LNH + Q   +  ++  LE   +EL S+  +  S K+     D  M  + +    +  KP +   LV D+  + V L+  + AK DKL +L  +WDNFK ++ +L + +      +Q+I     + + L  A   ++EL  +   +   KD   R G  LV  D + V+ +Q  LS A + W+KV  LL+EQQ K   + A W+Q L  K+ +   + E     + ++  P     D+ Q    +DK +K  D LKK +   E    K  QLI+E   +PNF    + +D+ +V+K+Y      +       E+++++W Q ++   +L+ W+ DT D    A  ++ D E  + KL++Y  +LP+    +  ++ K   L++ N+   I  L +    +D  F  V+     L+      ++    IR ++K   D ++ +RE  + C+D TG +++IL R+    +L+ E +    ++  +E +I  +S ++ A   + L KEL +L  R D V+S   K +  L   L K Y EK  +  + +A+ KEK  WC+P+Q  D+++LE KM +L ++                +AD   + AD D   ++  TV+          DRDK    +D +    AK+K  +   +  W+  +L+S+ +  WLK +E   R E +    L   +   +++ QL+  +    + L++L+   + ++ +SP++RV Q    L  RY+A    + + L+RL++L+ ++      + E E
Sbjct:  171 RFPSEESLSQRRGSSPQNPGSSDERTPAEWYAEYRNQSFQNVTARMEYNRSRSEYDAHIAQIRDEQERVQKKTFVNWINSYLLKRVPPLRIEDLIEDLKDGTRLLALLEVLSGEKLPVERGRNLKRPHFLSNANTALQFLQSKKIKLVNINSSDLVDGRPPVVLGLIWTIILYFQIEENTRALEYLGQTWGS-------QSSLESLGTQGSAASERRRQSSEKWKQGARKTLLQWVTNALPKDAGIHVRDFGESWRDGNAFLAIIDAIKANLVNIAAMREASNKARLETAFQVAETELGIARLLDPEDVDVPQPDEKSIMTYVAQFLHKYPEPRSA-GPDTFAAVQREYDTLLEWLNDRVRHLDQLNRTNA-FPPSYPEYMLFKTETDDKGVIYNKLQGLVDTPSMISITRDSWKQIETLWKKMESLMLRWLWSLDSTLPGEFGEVGRWLAQAESLLMSDNNIPEEMTEETASIISNKLEDHKKFFLDLPAM-TELLQRAKNSPLAPKIPPEQLQNMTSRLDSLPSRAAKRRIRLKFLEHKCCLIAFLFLVETKLKGWSVKYGTEEKVHQMLEQYRNFVSRNRIFQEFQKAYMDMQQVVDEYKREGEVDHEENINIDRFMRQTGEKWKNVSMDLRCVQSMLEEVVAYWRRWTTVSDEFESWLQRAEPALELPEEEKMEFFQDISVWKDKHQQLSDTVSFLIATSDDQVALQLKNRYTNLSARWEKLFPEAKQYMHAGDLLRNRKDYRAGVETLQSWLRNAESILSTTQLSSTEQIKAYGEQLKVLHNEVEGIEDLFKNISKKFQTLIQDLSRDEVDKMMNTLKKEKEALVRVRALIPMQLHLYHQLLVQQESLEAGQKEISNWLDDAERLLAGINLSGGRDSALAQLDRHKAFFSRTLYYKSMLESKNKVFSSIVKSVDSHANVDTAEGGAALRELNERFAQVSQSAQLAEQRLQEAVRCWTKFRECERQISEWLSAAETLINDKHIDNRQSVEYHKNFFGKVNEKWIQDLVNTGQDLRNAIEQ--EAPIVEAVDFLQKRWKEVLTFAPLHLMRLEFRLDEATFLQYLKEIEVEINSEQQALIKNDEVGNILQRNKDFFVNRGIVLEVERCLQTLKKISTAYTQLKPSDSSLNEATQKAEHQWEDSAQRIEQLREQLRQVPEQWAAYREKFAEMVRWMDHVDTTLRTILQEVNTLEEFEQERSIFQKICREADSKREEMKWLVQTLDSLTSNRTDHAAVTEQNQLEQLITRYKNLIPTIEITMTKTDIYSKSYTYRKEVREVCTLLKKVRDQTSIQEASENPETLTTALTHQETRLSQLEQQRANIVSMLQRGKDLLKDQHAPTFVSTEVQHLEISWNDTYGQSIEALKVLRGSQKLWNSYQQQKDEILKLIEQADKELKNIGTGYHDSAQVSADLQSKQELSISLRKAAEEMLRKLRDIHANLSEATVPQKRPLLEKEVTDIEKKLHITLETVQERVVYLQQFNARWTKFQARLGELQTWTQQSAPQLIANIQETSTSPEERVRKTEILQKQIHEKITILKVLEEESRKLITDDSENVEAKALRSEIKDLQNAVQSLNASIVTQREIATKNLTTWKEYEVGIQQLKPWIEQAESKAASIGSKPTTLDQAIKMLETAKAFEKQCEEHLPRVQELSVISQQIAGRTAAPDEVDAVHTRWNAVHDVAIQTTTKLDKLVSSWKTFETEAKEFNVWLQDSEKTALVEPNVQTPETGKLENELARLKEFNKAISDRQAQLISLTQVSDHISHGLALEGATNLKGRVSELKTRVNKLADIVRHQINRVSDALLARQEFQMKITDFENWMSRLRTNIAEISEVNVDNVDTNLQSVHAYLQEHSEKQPCFASIYEEVKHLSAQGSPEEAKALNEIYTSLAKKYKALEDDLCQKKKGLEKWAELLSWHNETSAQLNHSKYQVEAHKPTIPDLERLSSELQSVHTKISSWKEQISIIDSSMGVRVRD---KQEKPLTASALVNDLEAKAVTLQTELTAKRDKLENLSARWDNFKKLQTKLTEEIVHTQTTLQEITYSVDSCEKLAPAVEKISELIEEHRHREPDKDTLHREGATLVKEDQRAVTNIQVVLSSADANWEKVNELLREQQSKYSEMNADWKQFLEAKEKLKKFVKESKALCQSVKEVP----NDITQANIALDKHKKAADILKKGKQFLEKMDAKAQQLIKEASLMPNFKSELIESDLADVRKEYQDAYSNIVERSQTYETQVIIWKQIDEAKYELTRWLSDTNDALTTACEHLPDAENGQTKLARYREELPAHQLLRQGISTKTEQLIKLNNNAEIPTLNSLNRLLDDQFKVVKASADKLESLTSSFNEKEKGIRQELKKFSDLISKIREDIIKCDDLTGENTKILGRINKCQELKNELEKCNYNLSDVEEKITSMSSEYPAITKSSLPKELQALQLRRDGVASHADKVSATLVAFLTKLYHEKFGALQRMVATHKEKAAWCEPEQSSDRYNLEVKMASLVDVE-------------TGIADCEARKADTDNSLKLLQTVESSETMEGLRADRDKVAADLDSLKTSYAKIKSVLEHNIALWQKYELMSENVVCWLKETENKIRIESSALLTLPDIENKINEIGQLQKAVSDYRRELDDLSALGEDIIKVSPESRVGQYIGHLNTRYQAVLKFLASHLERLEELSKSRDQYKSNVKELE 2835          

HSP 2 Score: 70.4774 bits (171), Expect = 9.059e-8
Identity = 185/944 (19.60%), Postives = 382/944 (40.47%), Query Frame = 0

Query: 2050 FHKNLGELKDWVGKAKGKLNQITKLDVSPDDRVKMTLELQQ--DVKCKMKQLEKLETDAHN---LFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAM---GLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNL-GDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEV--PFNHIDSSLENVHSLAQE---HADN----QSRIAKVEINAKNKILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFC-TWHAESD---SALNHLEQTLDNVT---SLDGLEIAL--------AELDSLAQQCQSKKN---DGANDEEMAAKSKTYVIQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKV--QKINVREST----IDCLKT----AASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDE----VHQAMEDL-EHTPIRSKKDVAQMVDKCRKGL-DTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKA---NVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQ 2938
            +H+  G L+  V   K K         S  DR  + +++    DV+  +   E  + D  N   L + V  + T+  L+ + + V  D+++L  +       +  ++ +W   +    N+  WL++ E K+ +    L    ++++ +NE+ QL++   +   +   L    +    +S    +   +  L +R QAV   +   + RLE+L K+   + +   E++ WI+     +     + G   I      L +LKA  +   + Q  L   ++  + +   ++ D  +V++ ++ +L+ Q+  L+D +      I  ++     F+D    V  W+ D  +++ +  ++         +L +  ++AQ+   H +     Q R+  +  +   ++L   I A  K   D+ D +   +K +    ++  T+    D   + +N     ++++T        +IAL        AE D + Q C  + N   +  +     A  K Y  Q         +  D L+R +  +       D+L+ L D+W  F+ V ++L  W++Q+  +V  Q +   E T    +  LKT     A+  AE +A  ++  +++ + E LG +               +S   +K+  + +  KE        +A +EQ +    +   + ++    +    +DL +H+ I     V Q   K  + L DT  K    FE       +L     T P+     +R  ++ ++  +    + L+     L+  ++ +A++    + L+ W++D +          + L+  + +L  +      +MS ++   +V  K   LV+         L   L+ + Q F N+  +   L E +E      +    + K   D L   REK + C D  G  SEI  RL  +  L+
Sbjct: 2611 YHEKFGALQRMVATHKEKAAWCEPEQSS--DRYNLEVKMASLVDVETGIADCEARKADTDNSLKLLQTVESSETMEGLRADRDKVAADLDSLKTSYAKIKSVLEHNIALWQKYELMSENVVCWLKETENKIRIESSALLTLPDIENKINEIGQLQKAVSDYRRELDDLSALGEDIIKVSPESRVGQYIGHLNTRYQAVLKFLASHLERLEELSKSRDQYKSNVKELEAWIQNAEKKLNVFREITGPKPITFYQSRLKELKAFAEEREKGQSILNRTAEAGEALYARITPDNREVIRAELRNLRNQVDALADRANVIYKRIESDMMHRSSFEDKYSQVKQWVIDAQAKLGDKQDLLASLQEKKLALHSYRTIAQDVNVHRNILQQLQDRLGTMSDDEATEMLGTVIEAYDKLSGDVEDRINIAEKHVANHEAYLQTFEKTRDWINTIVNEAAPIIEDLTIERDTAKAKIALIGNILQQKAEGDRVLQDCNQQLNIILEQTSITGHPALLKGYEEQKR-------IWDDFLKRCLGTR-------DRLNQLFDQWAEFEKVVERLEAWIKQMESQVKDQSLKSTEETKRAHLQKLKTLEEEIAAKGAEFNAAVEKSQTIEGESE-LGTQ---------------VSRLATKYQAIRNQAKE-------AVARYEQFVREHGVFNEKYNQFLKWITNVQDDLTKHSEIVGDLTVLQSRQKSIRDLGDTRTKENARFESVIDLGEKLY--AHTSPDGR-EIIRQQLRNLRTVWDGFSEDLQNATQKLDQCLMQFAEFSLSQEQLTTWLRDVERAMHQHTELKSSLQEKRAQLQNHKIMHQEIMSHQSLVESVCDKAQQLVDQTKDT---SLNVYLQSIKQLFHNIVAKSQDLLENLEDCVDKHNQFNVQCKNFTDWLNGEREKLLDCNDIAGERSEISRRLATLEALR 3509          

BLAST of PREDICTED: nesprin-1-like vs. nr
Match: gi|1000755556|ref|XP_015600714.1| (PREDICTED: uncharacterized protein LOC107270320 isoform X4 [Cephus cinctus])

HSP 1 Score: 1701.8 bits (4406), Expect = 0.000e+0
Identity = 963/2697 (35.71%), Postives = 1547/2697 (57.36%), Query Frame = 0

Query:  569 RAPSLEQIPSRPNSALANPESQ------EWYDKYQLKAFPHDSQFGEQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERLRQL-KSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDD-IPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAVGEPEDLQIELAKHKPFLAKTVNMQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFEL-NKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKL-NQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKVEINAKN----------KILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLE-QTLDNVTSLDGLEIALAELDSLAQQCQS-KKNDGANDEEMAAKSKTYVIQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQ---MVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKANVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKC--------LDRDKKEV-MDEI----AKLKIDISELLLSWRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNKGAQMDAINEYE 3227
            R PS E +  R  S+  NP S       EWY +Y+ ++F + +   E + ++  YD+HI QIRDEQERVQKKTFVNWINSYL KR+PP++IEDLI+ L+DGTRLLALLEVL GE+LP E+GR L+RPH+LSN NTAL++L+SK+IKLVNIN++D+VDGRP VVLGLIWTIILYFQIEENTR LE LGQ +GS         + ES   +G+    R+    EKWK GARK LL W    + +  G+ V DFG+SWRDGNAFLAII++I+   V++ +MR+ SN+ RLETAF VAE +LG+ RLLDPEDVDVP PDEKS+MTYVAQFLHKYPE   A G D F ++  +Y  L  WL  ++   +Q+  T    P +Y E+   K E  +K  IY +L+ L  +   + IT ++W +++  W+K+E+ +  W W LD+ LPGEFG++G WL Q E L+ SD+ IP  + EE A++++ K+EDHK FF DL A+ T+      NSP   +IP +QLQ+M  RL  +  +A  R +RLKFLEHKCC++AFL L E+KLK W++KYG EE+V Q++ QY+ FVS+N+IFQEFQKA+++ Q V  EYK++GE+D EE+ +IDRF+ +   +WKN S +LRCVQS+LEEV+ YW+RW       + ++  A  AL+  E+ + EFF D + WK+++  L DTV+FL+ATSD  V   L+ ++  +S  WE+LF +A+ ++ +GD+ R ++ Y+ G+E L  WLR AES+L+TTQ   +  IK   E L  LH+EV G+E+LFK++S+KFQ L+ +L  D+++ MM +LKK+KE LV +R++IP ++ L+H LL Q E+ + G++E+  W DD + L+  +   G  +    +L +HK F ++T+  +S+L+SK  VF  ++K+ +    +D ++    +  +N RF    ++ Q  E  +   ++ W KF + ++ I  WL  A+ L N K+I++ Q V+ H++ F    E  + + +   Q L   + +  +A + E    ++  W  V  +AP H ++ EFRL E    + +KEIE ++  E QA  K++ +  I+Q + D+F    I+ +    L  + +I   Y  L P D +L +     ++QW+   +RI+ + ++L Q+PEQW  Y +KF++MVRWMD VD +L  + +       FE E+  FQ++CR+ D++R++MKWLVQ LDSL S R D A ++EQ +LE LITRYK++IP IE+TM K DIYSKSY ++ E   V  LL  +++++         E +   +  +E  L QL+  R  ++S+LQ+G DL++D  AP F+  EV+ LE  WND Y + +E  K      K+W+ Y++ K  +L+LI+ ++ EL N     HD  +V   L+ K+ L   + KA E++L K+R++   LS A  P+++  L  E+  +E+KL   LE   E++ +L + + +WT+F   LGEL+ W  ++  +L   I +   SP++RV+ T  LQ+ +  K+  L+ LE ++  L  +         L+ E++ ++  V++LN ++  +     K+L  W   +  +  +KPW+E+AE K A    + T L  A+  L   K FE++       + + +     ++      D+VDA+ +R  AV     Q   +L+ LV +WK F+    E + W++          N+       L  EL +LK  N  ++ +Q +L+ L++  D ++  ++ + A  +K +V  LK +++ L+D  R +++ + + +   QEFQ  I D  +W+  L + I E++EV  +++D++L++VH+  QEH++ Q   A +    K+          K L+    ++ KKY  L D L  KKK L++W+   +WH E+ + LNH + Q   +  ++  LE   +EL S+  +  S K+     D  M  + +    +  KP +   LV D+  + V L+  + AK DKL +L  +WDNFK ++ +L + +      +Q+I     + + L  A   ++EL  +   +   KD   R G  LV  D + V+ +Q  LS A + W+KV  LL+EQQ K   + A W+Q L  K+ +   + E     + ++  P     D+ Q    +DK +K  D LKK +   E    K  QLI+E   +PNF    + +D+ +V+K+Y      +       E+++++W Q ++   +L+ W+ DT D    A  ++ D E  + KL++Y  +LP+    +  ++ K   L++ N+   I  L +    +D  F  V+     L+      ++    IR ++K   D ++ +RE  + C+D TG +++IL R+    +L+ E +    ++  +E +I  +S ++ A   + L KEL +L  R D V+S   K +  L   L K Y EK  +  + +A+ KEK  WC+P+Q  D+++LE KM +L ++                +AD   + AD D   ++  TV+          DRDK    +D +    AK+K  +   +  W+  +L+S+ +  WLK +E   R E +    L   +   +++ QL+  +    + L++L+   + ++ +SP++RV Q    L  RY+A    + + L+RL++L+ ++      + E E
Sbjct:  171 RFPSEESLSQRRGSSPQNPGSSDERTPAEWYAEYRNQSFQNVTARMEYNRSRSEYDAHIAQIRDEQERVQKKTFVNWINSYLLKRVPPLRIEDLIEDLKDGTRLLALLEVLSGEKLPVERGRNLKRPHFLSNANTALQFLQSKKIKLVNINSSDLVDGRPPVVLGLIWTIILYFQIEENTRALEYLGQTWGS-------QSSLESLGTQGSAASERRRQSSEKWKQGARKTLLQWVTNALPKDAGIHVRDFGESWRDGNAFLAIIDAIKANLVNIAAMREASNKARLETAFQVAETELGIARLLDPEDVDVPQPDEKSIMTYVAQFLHKYPEPRSA-GPDTFAAVQREYDTLLEWLNDRVRHLDQLNRTNA-FPPSYPEYMLFKTETDDKGVIYNKLQGLVDTPSMISITRDSWKQIETLWKKMESLMLRWLWSLDSTLPGEFGEVGRWLAQAESLLMSDNNIPEEMTEETASIISNKLEDHKKFFLDLPAM-TELLQRAKNSPLAPKIPPEQLQNMTSRLDSLPSRAAKRRIRLKFLEHKCCLIAFLFLVETKLKGWSVKYGTEEKVHQMLEQYRNFVSRNRIFQEFQKAYMDMQQVVDEYKREGEVDHEENINIDRFMRQTGEKWKNVSMDLRCVQSMLEEVVAYWRRWTTVSDEFESWLQRAEPALELPEEEKMEFFQDISVWKDKHQQLSDTVSFLIATSDDQVALQLKNRYTNLSARWEKLFPEAKQYMHAGDLLRNRKDYRAGVETLQSWLRNAESILSTTQLSSTEQIKAYGEQLKVLHNEVEGIEDLFKNISKKFQTLIQDLSRDEVDKMMNTLKKEKEALVRVRALIPMQLHLYHQLLVQQESLEAGQKEISNWLDDAERLLAGINLSGGRDSALAQLDRHKAFFSRTLYYKSMLESKNKVFSSIVKSVDSHANVDTAEGGAALRELNERFAQVSQSAQLAEQRLQEAVRCWTKFRECERQISEWLSAAETLINDKHIDNRQSVEYHKNFFGKVNEKWIQDLVNTGQDLRNAIEQ--EAPIVEAVDFLQKRWKEVLTFAPLHLMRLEFRLDEATFLQYLKEIEVEINSEQQALIKNDEVGNILQRNKDFFVNRGIVLEVERCLQTLKKISTAYTQLKPSDSSLNEATQKAEHQWEDSAQRIEQLREQLRQVPEQWAAYREKFAEMVRWMDHVDTTLRTILQEVNTLEEFEQERSIFQKICREADSKREEMKWLVQTLDSLTSNRTDHAAVTEQNQLEQLITRYKNLIPTIEITMTKTDIYSKSYTYRKEVREVCTLLKKVRDQTSIQEASENPETLTTALTHQETRLSQLEQQRANIVSMLQRGKDLLKDQHAPTFVSTEVQHLEISWNDTYGQSIEALKVLRGSQKLWNSYQQQKDEILKLIEQADKELKNIGTGYHDSAQVSADLQSKQELSISLRKAAEEMLRKLRDIHANLSEATVPQKRPLLEKEVTDIEKKLHITLETVQERVVYLQQFNARWTKFQARLGELQTWTQQSAPQLIANIQETSTSPEERVRKTEILQKQIHEKITILKVLEEESRKLITDDSENVEAKALRSEIKDLQNAVQSLNASIVTQREIATKNLTTWKEYEVGIQQLKPWIEQAESKAASIGSKPTTLDQAIKMLETAKAFEKQCEEHLPRVQELSVISQQIAGRTAAPDEVDAVHTRWNAVHDVAIQTTTKLDKLVSSWKTFETEAKEFNVWLQDSEKTALVEPNVQTPETGKLENELARLKEFNKAISDRQAQLISLTQVSDHISHGLALEGATNLKGRVSELKTRVNKLADIVRHQINRVSDALLARQEFQMKITDFENWMSRLRTNIAEISEVNVDNVDTNLQSVHAYLQEHSEKQPCFASIYEEVKHLSAQGSPEEAKALNEIYTSLAKKYKALEDDLCQKKKGLEKWAELLSWHNETSAQLNHSKYQVEAHKPTIPDLERLSSELQSVHTKISSWKEQISIIDSSMGVRVRD---KQEKPLTASALVNDLEAKAVTLQTELTAKRDKLENLSARWDNFKKLQTKLTEEIVHTQTTLQEITYSVDSCEKLAPAVEKISELIEEHRHREPDKDTLHREGATLVKEDQRAVTNIQVVLSSADANWEKVNELLREQQSKYSEMNADWKQFLEAKEKLKKFVKESKALCQSVKEVP----NDITQANIALDKHKKAADILKKGKQFLEKMDAKAQQLIKEASLMPNFKSELIESDLADVRKEYQDAYSNIVERSQTYETQVIIWKQIDEAKYELTRWLSDTNDALTTACEHLPDAENGQTKLARYREELPAHQLLRQGISTKTEQLIKLNNNAEIPTLNSLNRLLDDQFKVVKASADKLESLTSSFNEKEKGIRQELKKFSDLISKIREDIIKCDDLTGENTKILGRINKCQELKNELEKCNYNLSDVEEKITSMSSEYPAITKSSLPKELQALQLRRDGVASHADKVSATLVAFLTKLYHEKFGALQRMVATHKEKAAWCEPEQSSDRYNLEVKMASLVDVE-------------TGIADCEARKADTDNSLKLLQTVESSETMEGLRADRDKVAADLDSLKTSYAKIKSVLEHNIALWQKYELMSENVVCWLKETENKIRIESSALLTLPDIENKINEIGQLQKAVSDYRRELDDLSALGEDIIKVSPESRVGQYIGHLNTRYQAVLKFLASHLERLEELSKSRDQYKSNVKELE 2835          

HSP 2 Score: 70.4774 bits (171), Expect = 9.368e-8
Identity = 185/944 (19.60%), Postives = 382/944 (40.47%), Query Frame = 0

Query: 2050 FHKNLGELKDWVGKAKGKLNQITKLDVSPDDRVKMTLELQQ--DVKCKMKQLEKLETDAHN---LFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAM---GLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNL-GDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEV--PFNHIDSSLENVHSLAQE---HADN----QSRIAKVEINAKNKILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFC-TWHAESD---SALNHLEQTLDNVT---SLDGLEIAL--------AELDSLAQQCQSKKN---DGANDEEMAAKSKTYVIQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKV--QKINVREST----IDCLKT----AASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDE----VHQAMEDL-EHTPIRSKKDVAQMVDKCRKGL-DTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKA---NVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQ 2938
            +H+  G L+  V   K K         S  DR  + +++    DV+  +   E  + D  N   L + V  + T+  L+ + + V  D+++L  +       +  ++ +W   +    N+  WL++ E K+ +    L    ++++ +NE+ QL++   +   +   L    +    +S    +   +  L +R QAV   +   + RLE+L K+   + +   E++ WI+     +     + G   I      L +LKA  +   + Q  L   ++  + +   ++ D  +V++ ++ +L+ Q+  L+D +      I  ++     F+D    V  W+ D  +++ +  ++         +L +  ++AQ+   H +     Q R+  +  +   ++L   I A  K   D+ D +   +K +    ++  T+    D   + +N     ++++T        +IAL        AE D + Q C  + N   +  +     A  K Y  Q         +  D L+R +  +       D+L+ L D+W  F+ V ++L  W++Q+  +V  Q +   E T    +  LKT     A+  AE +A  ++  +++ + E LG +               +S   +K+  + +  KE        +A +EQ +    +   + ++    +    +DL +H+ I     V Q   K  + L DT  K    FE       +L     T P+     +R  ++ ++  +    + L+     L+  ++ +A++    + L+ W++D +          + L+  + +L  +      +MS ++   +V  K   LV+         L   L+ + Q F N+  +   L E +E      +    + K   D L   REK + C D  G  SEI  RL  +  L+
Sbjct: 2611 YHEKFGALQRMVATHKEKAAWCEPEQSS--DRYNLEVKMASLVDVETGIADCEARKADTDNSLKLLQTVESSETMEGLRADRDKVAADLDSLKTSYAKIKSVLEHNIALWQKYELMSENVVCWLKETENKIRIESSALLTLPDIENKINEIGQLQKAVSDYRRELDDLSALGEDIIKVSPESRVGQYIGHLNTRYQAVLKFLASHLERLEELSKSRDQYKSNVKELEAWIQNAEKKLNVFREITGPKPITFYQSRLKELKAFAEEREKGQSILNRTAEAGEALYARITPDNREVIRAELRNLRNQVDALADRANVIYKRIESDMMHRSSFEDKYSQVKQWVIDAQAKLGDKQDLLASLQEKKLALHSYRTIAQDVNVHRNILQQLQDRLGTMSDDEATEMLGTVIEAYDKLSGDVEDRINIAEKHVANHEAYLQTFEKTRDWINTIVNEAAPIIEDLTIERDTAKAKIALIGNILQQKAEGDRVLQDCNQQLNIILEQTSITGHPALLKGYEEQKR-------IWDDFLKRCLGTR-------DRLNQLFDQWAEFEKVVERLEAWIKQMESQVKDQSLKSTEETKRAHLQKLKTLEEEIAAKGAEFNAAVEKSQTIEGESE-LGTQ---------------VSRLATKYQAIRNQAKE-------AVARYEQFVREHGVFNEKYNQFLKWITNVQDDLTKHSEIVGDLTVLQSRQKSIRDLGDTRTKENARFESVIDLGEKLY--AHTSPDGR-EIIRQQLRNLRTVWDGFSEDLQNATQKLDQCLMQFAEFSLSQEQLTTWLRDVERAMHQHTELKSSLQEKRAQLQNHKIMHQEIMSHQSLVESVCDKAQQLVDQTKDT---SLNVYLQSIKQLFHNIVAKSQDLLENLEDCVDKHNQFNVQCKNFTDWLNGEREKLLDCNDIAGERSEISRRLATLEALR 3509          

BLAST of PREDICTED: nesprin-1-like vs. nr
Match: gi|1000755554|ref|XP_015600713.1| (PREDICTED: uncharacterized protein LOC107270320 isoform X3 [Cephus cinctus])

HSP 1 Score: 1695.63 bits (4390), Expect = 0.000e+0
Identity = 963/2702 (35.64%), Postives = 1547/2702 (57.25%), Query Frame = 0

Query:  569 RAPSLEQIPSRPNSALANPESQ------EWYDKYQLKAFPHDSQFGEQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQIEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERLRQL-KSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDD-IPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAVGEPEDLQIELAKHKPFLAKTVNMQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQ-----ELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFEL-NKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKL-NQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKVEINAKN----------KILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLE-QTLDNVTSLDGLEIALAELDSLAQQCQS-KKNDGANDEEMAAKSKTYVIQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQ---MVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKANVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKC--------LDRDKKEV-MDEI----AKLKIDISELLLSWRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNKGAQMDAINEYE 3227
            R PS E +  R  S+  NP S       EWY +Y+ ++F + +   E + ++  YD+HI QIRDEQERVQKKTFVNWINSYL KR+PP++IEDLI+ L+DGTRLLALLEVL GE+LP E+GR L+RPH+LSN NTAL++L+SK+IKLVNIN++D+VDGRP VVLGLIWTIILYFQIEENTR LE LGQ +GS         + ES   +G+    R+    EKWK GARK LL W    + +  G+ V DFG+SWRDGNAFLAII++I+   V++ +MR+ SN+ RLETAF VAE +LG+ RLLDPEDVDVP PDEKS+MTYVAQFLHKYPE   A G D F ++  +Y  L  WL  ++   +Q+  T    P +Y E+   K E  +K  IY +L+ L  +   + IT ++W +++  W+K+E+ +  W W LD+ LPGEFG++G WL Q E L+ SD+ IP  + EE A++++ K+EDHK FF DL A+ T+      NSP   +IP +QLQ+M  RL  +  +A  R +RLKFLEHKCC++AFL L E+KLK W++KYG EE+V Q++ QY+ FVS+N+IFQEFQKA+++ Q V  EYK++GE+D EE+ +IDRF+ +   +WKN S +LRCVQS+LEEV+ YW+RW       + ++  A  AL+  E+ + EFF D + WK+++  L DTV+FL+ATSD  V   L+ ++  +S  WE+LF +A+ ++ +GD+ R ++ Y+ G+E L  WLR AES+L+TTQ   +  IK   E L  LH+EV G+E+LFK++S+KFQ L+ +L  D+++ MM +LKK+KE LV +R++IP ++ L+H LL Q E+ + G++E+  W DD + L+  +   G  +    +L +HK F ++T+  +S+L+SK  VF  ++K+ +    +D ++    +  +N RF    ++ Q  E  +   ++ W KF + ++ I  WL  A+ L N K+I++ Q V+ H++ F    E  + + +   Q L   + +  +A + E    ++  W  V  +AP H ++ EFRL E    + +KEIE ++  E QA  K++ +  I+Q + D+F    I+ +    L  + +I   Y  L P D +L +     ++QW+   +RI+ + ++L Q+PEQW  Y +KF++MVRWMD VD +L  + +       FE E+  FQ     ++CR+ D++R++MKWLVQ LDSL S R D A ++EQ +LE LITRYK++IP IE+TM K DIYSKSY ++ E   V  LL  +++++         E +   +  +E  L QL+  R  ++S+LQ+G DL++D  AP F+  EV+ LE  WND Y + +E  K      K+W+ Y++ K  +L+LI+ ++ EL N     HD  +V   L+ K+ L   + KA E++L K+R++   LS A  P+++  L  E+  +E+KL   LE   E++ +L + + +WT+F   LGEL+ W  ++  +L   I +   SP++RV+ T  LQ+ +  K+  L+ LE ++  L  +         L+ E++ ++  V++LN ++  +     K+L  W   +  +  +KPW+E+AE K A    + T L  A+  L   K FE++       + + +     ++      D+VDA+ +R  AV     Q   +L+ LV +WK F+    E + W++          N+       L  EL +LK  N  ++ +Q +L+ L++  D ++  ++ + A  +K +V  LK +++ L+D  R +++ + + +   QEFQ  I D  +W+  L + I E++EV  +++D++L++VH+  QEH++ Q   A +    K+          K L+    ++ KKY  L D L  KKK L++W+   +WH E+ + LNH + Q   +  ++  LE   +EL S+  +  S K+     D  M  + +    +  KP +   LV D+  + V L+  + AK DKL +L  +WDNFK ++ +L + +      +Q+I     + + L  A   ++EL  +   +   KD   R G  LV  D + V+ +Q  LS A + W+KV  LL+EQQ K   + A W+Q L  K+ +   + E     + ++  P     D+ Q    +DK +K  D LKK +   E    K  QLI+E   +PNF    + +D+ +V+K+Y      +       E+++++W Q ++   +L+ W+ DT D    A  ++ D E  + KL++Y  +LP+    +  ++ K   L++ N+   I  L +    +D  F  V+     L+      ++    IR ++K   D ++ +RE  + C+D TG +++IL R+    +L+ E +    ++  +E +I  +S ++ A   + L KEL +L  R D V+S   K +  L   L K Y EK  +  + +A+ KEK  WC+P+Q  D+++LE KM +L ++                +AD   + AD D   ++  TV+          DRDK    +D +    AK+K  +   +  W+  +L+S+ +  WLK +E   R E +    L   +   +++ QL+  +    + L++L+   + ++ +SP++RV Q    L  RY+A    + + L+RL++L+ ++      + E E
Sbjct:  171 RFPSEESLSQRRGSSPQNPGSSDERTPAEWYAEYRNQSFQNVTARMEYNRSRSEYDAHIAQIRDEQERVQKKTFVNWINSYLLKRVPPLRIEDLIEDLKDGTRLLALLEVLSGEKLPVERGRNLKRPHFLSNANTALQFLQSKKIKLVNINSSDLVDGRPPVVLGLIWTIILYFQIEENTRALEYLGQTWGS-------QSSLESLGTQGSAASERRRQSSEKWKQGARKTLLQWVTNALPKDAGIHVRDFGESWRDGNAFLAIIDAIKANLVNIAAMREASNKARLETAFQVAETELGIARLLDPEDVDVPQPDEKSIMTYVAQFLHKYPEPRSA-GPDTFAAVQREYDTLLEWLNDRVRHLDQLNRTNA-FPPSYPEYMLFKTETDDKGVIYNKLQGLVDTPSMISITRDSWKQIETLWKKMESLMLRWLWSLDSTLPGEFGEVGRWLAQAESLLMSDNNIPEEMTEETASIISNKLEDHKKFFLDLPAM-TELLQRAKNSPLAPKIPPEQLQNMTSRLDSLPSRAAKRRIRLKFLEHKCCLIAFLFLVETKLKGWSVKYGTEEKVHQMLEQYRNFVSRNRIFQEFQKAYMDMQQVVDEYKREGEVDHEENINIDRFMRQTGEKWKNVSMDLRCVQSMLEEVVAYWRRWTTVSDEFESWLQRAEPALELPEEEKMEFFQDISVWKDKHQQLSDTVSFLIATSDDQVALQLKNRYTNLSARWEKLFPEAKQYMHAGDLLRNRKDYRAGVETLQSWLRNAESILSTTQLSSTEQIKAYGEQLKVLHNEVEGIEDLFKNISKKFQTLIQDLSRDEVDKMMNTLKKEKEALVRVRALIPMQLHLYHQLLVQQESLEAGQKEISNWLDDAERLLAGINLSGGRDSALAQLDRHKAFFSRTLYYKSMLESKNKVFSSIVKSVDSHANVDTAEGGAALRELNERFAQVSQSAQLAEQRLQEAVRCWTKFRECERQISEWLSAAETLINDKHIDNRQSVEYHKNFFGKVNEKWIQDLVNTGQDLRNAIEQ--EAPIVEAVDFLQKRWKEVLTFAPLHLMRLEFRLDEATFLQYLKEIEVEINSEQQALIKNDEVGNILQRNKDFFVNRGIVLEVERCLQTLKKISTAYTQLKPSDSSLNEATQKAEHQWEDSAQRIEQLREQLRQVPEQWAAYREKFAEMVRWMDHVDTTLRTILQEVNTLEEFEQERSIFQLQTLTKICREADSKREEMKWLVQTLDSLTSNRTDHAAVTEQNQLEQLITRYKNLIPTIEITMTKTDIYSKSYTYRKEVREVCTLLKKVRDQTSIQEASENPETLTTALTHQETRLSQLEQQRANIVSMLQRGKDLLKDQHAPTFVSTEVQHLEISWNDTYGQSIEALKVLRGSQKLWNSYQQQKDEILKLIEQADKELKNIGTGYHDSAQVSADLQSKQELSISLRKAAEEMLRKLRDIHANLSEATVPQKRPLLEKEVTDIEKKLHITLETVQERVVYLQQFNARWTKFQARLGELQTWTQQSAPQLIANIQETSTSPEERVRKTEILQKQIHEKITILKVLEEESRKLITDDSENVEAKALRSEIKDLQNAVQSLNASIVTQREIATKNLTTWKEYEVGIQQLKPWIEQAESKAASIGSKPTTLDQAIKMLETAKAFEKQCEEHLPRVQELSVISQQIAGRTAAPDEVDAVHTRWNAVHDVAIQTTTKLDKLVSSWKTFETEAKEFNVWLQDSEKTALVEPNVQTPETGKLENELARLKEFNKAISDRQAQLISLTQVSDHISHGLALEGATNLKGRVSELKTRVNKLADIVRHQINRVSDALLARQEFQMKITDFENWMSRLRTNIAEISEVNVDNVDTNLQSVHAYLQEHSEKQPCFASIYEEVKHLSAQGSPEEAKALNEIYTSLAKKYKALEDDLCQKKKGLEKWAELLSWHNETSAQLNHSKYQVEAHKPTIPDLERLSSELQSVHTKISSWKEQISIIDSSMGVRVRD---KQEKPLTASALVNDLEAKAVTLQTELTAKRDKLENLSARWDNFKKLQTKLTEEIVHTQTTLQEITYSVDSCEKLAPAVEKISELIEEHRHREPDKDTLHREGATLVKEDQRAVTNIQVVLSSADANWEKVNELLREQQSKYSEMNADWKQFLEAKEKLKKFVKESKALCQSVKEVP----NDITQANIALDKHKKAADILKKGKQFLEKMDAKAQQLIKEASLMPNFKSELIESDLADVRKEYQDAYSNIVERSQTYETQVIIWKQIDEAKYELTRWLSDTNDALTTACEHLPDAENGQTKLARYREELPAHQLLRQGISTKTEQLIKLNNNAEIPTLNSLNRLLDDQFKVVKASADKLESLTSSFNEKEKGIRQELKKFSDLISKIREDIIKCDDLTGENTKILGRINKCQELKNELEKCNYNLSDVEEKITSMSSEYPAITKSSLPKELQALQLRRDGVASHADKVSATLVAFLTKLYHEKFGALQRMVATHKEKAAWCEPEQSSDRYNLEVKMASLVDVE-------------TGIADCEARKADTDNSLKLLQTVESSETMEGLRADRDKVAADLDSLKTSYAKIKSVLEHNIALWQKYELMSENVVCWLKETENKIRIESSALLTLPDIENKINEIGQLQKAVSDYRRELDDLSALGEDIIKVSPESRVGQYIGHLNTRYQAVLKFLASHLERLEELSKSRDQYKSNVKELE 2840          

HSP 2 Score: 70.4774 bits (171), Expect = 1.002e-7
Identity = 185/944 (19.60%), Postives = 382/944 (40.47%), Query Frame = 0

Query: 2050 FHKNLGELKDWVGKAKGKLNQITKLDVSPDDRVKMTLELQQ--DVKCKMKQLEKLETDAHN---LFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAM---GLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNL-GDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEV--PFNHIDSSLENVHSLAQE---HADN----QSRIAKVEINAKNKILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFC-TWHAESD---SALNHLEQTLDNVT---SLDGLEIAL--------AELDSLAQQCQSKKN---DGANDEEMAAKSKTYVIQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKV--QKINVREST----IDCLKT----AASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDE----VHQAMEDL-EHTPIRSKKDVAQMVDKCRKGL-DTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKA---NVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQ 2938
            +H+  G L+  V   K K         S  DR  + +++    DV+  +   E  + D  N   L + V  + T+  L+ + + V  D+++L  +       +  ++ +W   +    N+  WL++ E K+ +    L    ++++ +NE+ QL++   +   +   L    +    +S    +   +  L +R QAV   +   + RLE+L K+   + +   E++ WI+     +     + G   I      L +LKA  +   + Q  L   ++  + +   ++ D  +V++ ++ +L+ Q+  L+D +      I  ++     F+D    V  W+ D  +++ +  ++         +L +  ++AQ+   H +     Q R+  +  +   ++L   I A  K   D+ D +   +K +    ++  T+    D   + +N     ++++T        +IAL        AE D + Q C  + N   +  +     A  K Y  Q         +  D L+R +  +       D+L+ L D+W  F+ V ++L  W++Q+  +V  Q +   E T    +  LKT     A+  AE +A  ++  +++ + E LG +               +S   +K+  + +  KE        +A +EQ +    +   + ++    +    +DL +H+ I     V Q   K  + L DT  K    FE       +L     T P+     +R  ++ ++  +    + L+     L+  ++ +A++    + L+ W++D +          + L+  + +L  +      +MS ++   +V  K   LV+         L   L+ + Q F N+  +   L E +E      +    + K   D L   REK + C D  G  SEI  RL  +  L+
Sbjct: 2616 YHEKFGALQRMVATHKEKAAWCEPEQSS--DRYNLEVKMASLVDVETGIADCEARKADTDNSLKLLQTVESSETMEGLRADRDKVAADLDSLKTSYAKIKSVLEHNIALWQKYELMSENVVCWLKETENKIRIESSALLTLPDIENKINEIGQLQKAVSDYRRELDDLSALGEDIIKVSPESRVGQYIGHLNTRYQAVLKFLASHLERLEELSKSRDQYKSNVKELEAWIQNAEKKLNVFREITGPKPITFYQSRLKELKAFAEEREKGQSILNRTAEAGEALYARITPDNREVIRAELRNLRNQVDALADRANVIYKRIESDMMHRSSFEDKYSQVKQWVIDAQAKLGDKQDLLASLQEKKLALHSYRTIAQDVNVHRNILQQLQDRLGTMSDDEATEMLGTVIEAYDKLSGDVEDRINIAEKHVANHEAYLQTFEKTRDWINTIVNEAAPIIEDLTIERDTAKAKIALIGNILQQKAEGDRVLQDCNQQLNIILEQTSITGHPALLKGYEEQKR-------IWDDFLKRCLGTR-------DRLNQLFDQWAEFEKVVERLEAWIKQMESQVKDQSLKSTEETKRAHLQKLKTLEEEIAAKGAEFNAAVEKSQTIEGESE-LGTQ---------------VSRLATKYQAIRNQAKE-------AVARYEQFVREHGVFNEKYNQFLKWITNVQDDLTKHSEIVGDLTVLQSRQKSIRDLGDTRTKENARFESVIDLGEKLY--AHTSPDGR-EIIRQQLRNLRTVWDGFSEDLQNATQKLDQCLMQFAEFSLSQEQLTTWLRDVERAMHQHTELKSSLQEKRAQLQNHKIMHQEIMSHQSLVESVCDKAQQLVDQTKDT---SLNVYLQSIKQLFHNIVAKSQDLLENLEDCVDKHNQFNVQCKNFTDWLNGEREKLLDCNDIAGERSEISRRLATLEALR 3514          

BLAST of PREDICTED: nesprin-1-like vs. nr
Match: gi|1000755552|ref|XP_015600712.1| (PREDICTED: uncharacterized protein LOC107270320 isoform X2 [Cephus cinctus])

HSP 1 Score: 1694.86 bits (4388), Expect = 0.000e+0
Identity = 963/2705 (35.60%), Postives = 1547/2705 (57.19%), Query Frame = 0

Query:  569 RAPSLEQIPSRPNSALANPESQ------EWYDKYQLKAFPHDSQFGEQDYAKHNYDSHIHQIRDEQERVQKKTFVNWINSYLAKRIPPMKIEDLIDGLRDGTRLLALLEVLGGERLPTEKGRVLRRPHYLSNVNTALEYLRSKRIKLVNINAADVVDGRPAVVLGLIWTIILYFQ--------IEENTRVLESLGQRYGSPAVGRSGYGTGESEDDRGTPGGVRKLSVPEKWKLGARKALLHWCQTQIGQKFGVGVTDFGKSWRDGNAFLAIINSIQPGAVDMTSMRQESNQTRLETAFYVAEVKLGVTRLLDPEDVDVPSPDEKSVMTYVAQFLHKYPEGAVAKGSDRFGSLDSQYQELRAWLTTKISWFEQMQVTKGQLPMNYDEFQKCKAEMKNKQEIYERLRQL-KSNKQLGITPEAWHELDNNWQKVETQVRHWQWLLDTALPGEFGQIGEWLNQGEGLINSDD-IPAMLNEEAAAVLNQKIEDHKAFFADLSAVQTQFETAVTNSPYVEQIPKDQLQSMAQRLRDIGPKADVRAVRLKFLEHKCCIVAFLMLTESKLKNWTIKYGREERVQQIMSQYKTFVSKNKIFQEFQKAFVEFQDVSAEYKKDGEIDTEESQSIDRFIDEVANRWKNTSTELRCVQSLLEEVLTYWKRWNGNYQPLQCYIIDAFEALKKNEDAQQEFFHDFANWKERYILLEDTVAFLVATSDAAVGQDLREKFMIVSNNWEQLFKQAEGFLVSGDISRKKRVYQEGLEQLDQWLRKAESVLNTTQQVESGSIKKTLEVLMELHSEVGGMEELFKSLSRKFQQLVPELDNDDIENMMFSLKKKKENLVIIRSMIPTRIQLFHHLLSQVEAFDMGERELLQWCDDVDSLVKTVQAVGEPEDLQIELAKHKPFLAKTVNMQSLLQSKRNVFQGVLKNTEGKEGLDASDVSRRMDYINGRFEVALRAVQQFETNMTSGMQLWDKFLDAQKAIVCWLKEAKNLFNMKNINSPQVVQQHRSHFAPGCEAILNNYLIMAQQLEPFLSESDKAKVREDTGSIKAEWDNVHQYAPHHALKTEFRLHEDKLNKSIKEIENKLQDESQAFQKSENIPQIIQEHTDYFNRTNIIQDARESLMKMAEIVKRYKSLAPEDRNLEDILNTKQNQWDLIYERIQAVLKRLEQIPEQWREYEKKFSQMVRWMDTVDNSLAKMFKGQADANTFEGEKQGFQELCRDVDNRRDDMKWLVQKLDSLVSQRADDAGLSEQKRLEGLITRYKSMIPVIEMTMQKVDIYSKSYHFQAEAHNVHNLLNDIQNKSIEDNFPATEEDVQALVNREEAILQQLDDHRGPVLSLLQKGLDLMRDPKAPPFLKEEVKALESMWNDCYSRCVERRKNFTEQLKVWHEYKENKVHMLRLIDDSEFEL-NKAVPQHDHKEVKLQLRVKKNLKEEIGKATEDILGKMRELADALSTAANPEQQSALSDEMNAMEQKLRDLLEECDEKIQHLGELDVKWTEFHKNLGELKDWVGKAKGKL-NQITKLDVSPDDRVKMTLELQQDVKCKMKQLEKLETDAHNLFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAMGLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNLGDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEVPFNHIDSSLENVHSLAQEHADNQSRIAKVEINAKN----------KILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFCTWHAESDSALNHLE-QTLDNVTSLDGLEIALAELDSLAQQCQS-KKNDGANDEEMAAKSKTYVIQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKVQKINVRESTIDCLKTAASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDEVHQAMEDLEHTPIRSKKDVAQ---MVDKCRKGLDTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKANVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQGEADDFEESMGAIEGEIKDLSHKFQAADVAPLAKELSSLAKRYDVVSSQIAKTALALYNVLEKHYVEKVQSEIKSIASAKEKLLWCQPDQKGDKFSLETKMDALNELARKLELQQDPSSSLGHLADLLLQVADDDKGHEVKDTVKC--------LDRDKKEV-MDEI----AKLKIDISELLLSWRNVDLLSDKLADWLKNSETIFREEIACPTELETCKEISDKLEQLKTELHAQDQPLEELNQATDVLMAMSPDARVRQQTNKLKNRYEAFKVSVDTQLQRLQDLADNKGAQMDAINEYE 3227
            R PS E +  R  S+  NP S       EWY +Y+ ++F + +   E + ++  YD+HI QIRDEQERVQKKTFVNWINSYL KR+PP++IEDLI+ L+DGTRLLALLEVL GE+LP E+GR L+RPH+LSN NTAL++L+SK+IKLVNIN++D+VDGRP VVLGLIWTIILYFQ        IEENTR LE LGQ +GS         + ES   +G+    R+    EKWK GARK LL W    + +  G+ V DFG+SWRDGNAFLAII++I+   V++ +MR+ SN+ RLETAF VAE +LG+ RLLDPEDVDVP PDEKS+MTYVAQFLHKYPE   A G D F ++  +Y  L  WL  ++   +Q+  T    P +Y E+   K E  +K  IY +L+ L  +   + IT ++W +++  W+K+E+ +  W W LD+ LPGEFG++G WL Q E L+ SD+ IP  + EE A++++ K+EDHK FF DL A+ T+      NSP   +IP +QLQ+M  RL  +  +A  R +RLKFLEHKCC++AFL L E+KLK W++KYG EE+V Q++ QY+ FVS+N+IFQEFQKA+++ Q V  EYK++GE+D EE+ +IDRF+ +   +WKN S +LRCVQS+LEEV+ YW+RW       + ++  A  AL+  E+ + EFF D + WK+++  L DTV+FL+ATSD  V   L+ ++  +S  WE+LF +A+ ++ +GD+ R ++ Y+ G+E L  WLR AES+L+TTQ   +  IK   E L  LH+EV G+E+LFK++S+KFQ L+ +L  D+++ MM +LKK+KE LV +R++IP ++ L+H LL Q E+ + G++E+  W DD + L+  +   G  +    +L +HK F ++T+  +S+L+SK  VF  ++K+ +    +D ++    +  +N RF    ++ Q  E  +   ++ W KF + ++ I  WL  A+ L N K+I++ Q V+ H++ F    E  + + +   Q L   + +  +A + E    ++  W  V  +AP H ++ EFRL E    + +KEIE ++  E QA  K++ +  I+Q + D+F    I+ +    L  + +I   Y  L P D +L +     ++QW+   +RI+ + ++L Q+PEQW  Y +KF++MVRWMD VD +L  + +       FE E+  FQ++CR+ D++R++MKWLVQ LDSL S R D A ++EQ +LE LITRYK++IP IE+TM K DIYSKSY ++ E   V  LL  +++++         E +   +  +E  L QL+  R  ++S+LQ+G DL++D  AP F+  EV+ LE  WND Y + +E  K      K+W+ Y++ K  +L+LI+ ++ EL N     HD  +V   L+ K+ L   + KA E++L K+R++   LS A  P+++  L  E+  +E+KL   LE   E++ +L + + +WT+F   LGEL+ W  ++  +L   I +   SP++RV+ T  LQ+ +  K+  L+ LE ++  L  +         L+ E++ ++  V++LN ++  +     K+L  W   +  +  +KPW+E+AE K A    + T L  A+  L   K FE++       + + +     ++      D+VDA+ +R  AV     Q   +L+ LV +WK F+    E + W++          N+       L  EL +LK  N  ++ +Q +L+ L++  D ++  ++ + A  +K +V  LK +++ L+D  R +++ + + +   QEFQ  I D  +W+  L + I E++EV  +++D++L++VH+  QEH++ Q   A +    K+          K L+    ++ KKY  L D L  KKK L++W+   +WH E+ + LNH + Q   +  ++  LE   +EL S+  +  S K+     D  M  + +    +  KP +   LV D+  + V L+  + AK DKL +L  +WDNFK ++ +L + +      +Q+I     + + L  A   ++EL  +   +   KD   R G  LV  D + V+ +Q  LS A + W+KV  LL+EQQ K   + A W+Q L  K+ +   + E     + ++  P     D+ Q    +DK +K  D LKK +   E    K  QLI+E   +PNF    + +D+ +V+K+Y      +       E+++++W Q ++   +L+ W+ DT D    A  ++ D E  + KL++Y  +LP+    +  ++ K   L++ N+   I  L +    +D  F  V+     L+      ++    IR ++K   D ++ +RE  + C+D TG +++IL R+    +L+ E +    ++  +E +I  +S ++ A   + L KEL +L  R D V+S   K +  L   L K Y EK  +  + +A+ KEK  WC+P+Q  D+++LE KM +L ++                +AD   + AD D   ++  TV+          DRDK    +D +    AK+K  +   +  W+  +L+S+ +  WLK +E   R E +    L   +   +++ QL+  +    + L++L+   + ++ +SP++RV Q    L  RY+A    + + L+RL++L+ ++      + E E
Sbjct:  171 RFPSEESLSQRRGSSPQNPGSSDERTPAEWYAEYRNQSFQNVTARMEYNRSRSEYDAHIAQIRDEQERVQKKTFVNWINSYLLKRVPPLRIEDLIEDLKDGTRLLALLEVLSGEKLPVERGRNLKRPHFLSNANTALQFLQSKKIKLVNINSSDLVDGRPPVVLGLIWTIILYFQLRSKDRRRIEENTRALEYLGQTWGS-------QSSLESLGTQGSAASERRRQSSEKWKQGARKTLLQWVTNALPKDAGIHVRDFGESWRDGNAFLAIIDAIKANLVNIAAMREASNKARLETAFQVAETELGIARLLDPEDVDVPQPDEKSIMTYVAQFLHKYPEPRSA-GPDTFAAVQREYDTLLEWLNDRVRHLDQLNRTNA-FPPSYPEYMLFKTETDDKGVIYNKLQGLVDTPSMISITRDSWKQIETLWKKMESLMLRWLWSLDSTLPGEFGEVGRWLAQAESLLMSDNNIPEEMTEETASIISNKLEDHKKFFLDLPAM-TELLQRAKNSPLAPKIPPEQLQNMTSRLDSLPSRAAKRRIRLKFLEHKCCLIAFLFLVETKLKGWSVKYGTEEKVHQMLEQYRNFVSRNRIFQEFQKAYMDMQQVVDEYKREGEVDHEENINIDRFMRQTGEKWKNVSMDLRCVQSMLEEVVAYWRRWTTVSDEFESWLQRAEPALELPEEEKMEFFQDISVWKDKHQQLSDTVSFLIATSDDQVALQLKNRYTNLSARWEKLFPEAKQYMHAGDLLRNRKDYRAGVETLQSWLRNAESILSTTQLSSTEQIKAYGEQLKVLHNEVEGIEDLFKNISKKFQTLIQDLSRDEVDKMMNTLKKEKEALVRVRALIPMQLHLYHQLLVQQESLEAGQKEISNWLDDAERLLAGINLSGGRDSALAQLDRHKAFFSRTLYYKSMLESKNKVFSSIVKSVDSHANVDTAEGGAALRELNERFAQVSQSAQLAEQRLQEAVRCWTKFRECERQISEWLSAAETLINDKHIDNRQSVEYHKNFFGKVNEKWIQDLVNTGQDLRNAIEQ--EAPIVEAVDFLQKRWKEVLTFAPLHLMRLEFRLDEATFLQYLKEIEVEINSEQQALIKNDEVGNILQRNKDFFVNRGIVLEVERCLQTLKKISTAYTQLKPSDSSLNEATQKAEHQWEDSAQRIEQLREQLRQVPEQWAAYREKFAEMVRWMDHVDTTLRTILQEVNTLEEFEQERSIFQKICREADSKREEMKWLVQTLDSLTSNRTDHAAVTEQNQLEQLITRYKNLIPTIEITMTKTDIYSKSYTYRKEVREVCTLLKKVRDQTSIQEASENPETLTTALTHQETRLSQLEQQRANIVSMLQRGKDLLKDQHAPTFVSTEVQHLEISWNDTYGQSIEALKVLRGSQKLWNSYQQQKDEILKLIEQADKELKNIGTGYHDSAQVSADLQSKQELSISLRKAAEEMLRKLRDIHANLSEATVPQKRPLLEKEVTDIEKKLHITLETVQERVVYLQQFNARWTKFQARLGELQTWTQQSAPQLIANIQETSTSPEERVRKTEILQKQIHEKITILKVLEEESRKLITDDSENVEAKALRSEIKDLQNAVQSLNASIVTQREIATKNLTTWKEYEVGIQQLKPWIEQAESKAASIGSKPTTLDQAIKMLETAKAFEKQCEEHLPRVQELSVISQQIAGRTAAPDEVDAVHTRWNAVHDVAIQTTTKLDKLVSSWKTFETEAKEFNVWLQDSEKTALVEPNVQTPETGKLENELARLKEFNKAISDRQAQLISLTQVSDHISHGLALEGATNLKGRVSELKTRVNKLADIVRHQINRVSDALLARQEFQMKITDFENWMSRLRTNIAEISEVNVDNVDTNLQSVHAYLQEHSEKQPCFASIYEEVKHLSAQGSPEEAKALNEIYTSLAKKYKALEDDLCQKKKGLEKWAELLSWHNETSAQLNHSKYQVEAHKPTIPDLERLSSELQSVHTKISSWKEQISIIDSSMGVRVRD---KQEKPLTASALVNDLEAKAVTLQTELTAKRDKLENLSARWDNFKKLQTKLTEEIVHTQTTLQEITYSVDSCEKLAPAVEKISELIEEHRHREPDKDTLHREGATLVKEDQRAVTNIQVVLSSADANWEKVNELLREQQSKYSEMNADWKQFLEAKEKLKKFVKESKALCQSVKEVP----NDITQANIALDKHKKAADILKKGKQFLEKMDAKAQQLIKEASLMPNFKSELIESDLADVRKEYQDAYSNIVERSQTYETQVIIWKQIDEAKYELTRWLSDTNDALTTACEHLPDAENGQTKLARYREELPAHQLLRQGISTKTEQLIKLNNNAEIPTLNSLNRLLDDQFKVVKASADKLESLTSSFNEKEKGIRQELKKFSDLISKIREDIIKCDDLTGENTKILGRINKCQELKNELEKCNYNLSDVEEKITSMSSEYPAITKSSLPKELQALQLRRDGVASHADKVSATLVAFLTKLYHEKFGALQRMVATHKEKAAWCEPEQSSDRYNLEVKMASLVDVE-------------TGIADCEARKADTDNSLKLLQTVESSETMEGLRADRDKVAADLDSLKTSYAKIKSVLEHNIALWQKYELMSENVVCWLKETENKIRIESSALLTLPDIENKINEIGQLQKAVSDYRRELDDLSALGEDIIKVSPESRVGQYIGHLNTRYQAVLKFLASHLERLEELSKSRDQYKSNVKELE 2843          

HSP 2 Score: 70.8626 bits (172), Expect = 8.402e-8
Identity = 185/944 (19.60%), Postives = 382/944 (40.47%), Query Frame = 0

Query: 2050 FHKNLGELKDWVGKAKGKLNQITKLDVSPDDRVKMTLELQQ--DVKCKMKQLEKLETDAHN---LFENVPHTATVNDLKIEVETVKKDVEALNMNVDAKSVAIAKDLDIWNACQNDLLNIKPWLEKAEMKVAM---GLPRHTNLQDALNELTQLKRFEEEIFGKEKLLVQAADKGANLSCGQGISDDVDALKSRLQAVKANVTQWIPRLEDLVKTWKHFDNGYDEIDGWIEAKMTNMKGHDNL-GDLSIDALTGELDQLKALNDNLTQKQVELLGLSKDCDIVAMNMSQDTAQVVKQKVGHLKGQLSDLSDSSRAKMSNIMENIQTMQEFQDDIGDVGSWLKDLNSRIVELNEV--PFNHIDSSLENVHSLAQE---HADN----QSRIAKVEINAKNKILSNSIRAMQKKYLDLGDLLENKKKSLQRWSSFC-TWHAESD---SALNHLEQTLDNVT---SLDGLEIAL--------AELDSLAQQCQSKKN---DGANDEEMAAKSKTYVIQNGKPTSVLLLVADILQRIVQLKDMIEAKEDKLHSLEDKWDNFKDVEQQLADWLQQVLQKV--QKINVREST----IDCLKT----AASAVAELDAQCDQKASLKDDYERLGRELVMSDPQQVSVVQDALSEATSKWDKVTSLLKEQQGKSQSLIALWEQCLANKDIVMTQLDE----VHQAMEDL-EHTPIRSKKDVAQMVDKCRKGL-DTLKKVRHPFEVFYKKQTQLIQELQTVPNFDIAPLRTDMQEVQKKYSYLGKTLKGNLNGLESKMVLWAQWEQLVDDLSGWIQDTQDGFECAATNIADLETAKGKLSKYDNDLPSMMSQKA---NVAAKMALLVESNHRKPIEGLQATLEDMDQGFANVEGQYTTLKEAIEGLDKTADAIRAKIKAHFDDLATLREKAMTCEDFTGPDSEILERLLLINQLQ 2938
            +H+  G L+  V   K K         S  DR  + +++    DV+  +   E  + D  N   L + V  + T+  L+ + + V  D+++L  +       +  ++ +W   +    N+  WL++ E K+ +    L    ++++ +NE+ QL++   +   +   L    +    +S    +   +  L +R QAV   +   + RLE+L K+   + +   E++ WI+     +     + G   I      L +LKA  +   + Q  L   ++  + +   ++ D  +V++ ++ +L+ Q+  L+D +      I  ++     F+D    V  W+ D  +++ +  ++         +L +  ++AQ+   H +     Q R+  +  +   ++L   I A  K   D+ D +   +K +    ++  T+    D   + +N     ++++T        +IAL        AE D + Q C  + N   +  +     A  K Y  Q         +  D L+R +  +       D+L+ L D+W  F+ V ++L  W++Q+  +V  Q +   E T    +  LKT     A+  AE +A  ++  +++ + E LG +               +S   +K+  + +  KE        +A +EQ +    +   + ++    +    +DL +H+ I     V Q   K  + L DT  K    FE       +L     T P+     +R  ++ ++  +    + L+     L+  ++ +A++    + L+ W++D +          + L+  + +L  +      +MS ++   +V  K   LV+         L   L+ + Q F N+  +   L E +E      +    + K   D L   REK + C D  G  SEI  RL  +  L+
Sbjct: 2619 YHEKFGALQRMVATHKEKAAWCEPEQSS--DRYNLEVKMASLVDVETGIADCEARKADTDNSLKLLQTVESSETMEGLRADRDKVAADLDSLKTSYAKIKSVLEHNIALWQKYELMSENVVCWLKETENKIRIESSALLTLPDIENKINEIGQLQKAVSDYRRELDDLSALGEDIIKVSPESRVGQYIGHLNTRYQAVLKFLASHLERLEELSKSRDQYKSNVKELEAWIQNAEKKLNVFREITGPKPITFYQSRLKELKAFAEEREKGQSILNRTAEAGEALYARITPDNREVIRAELRNLRNQVDALADRANVIYKRIESDMMHRSSFEDKYSQVKQWVIDAQAKLGDKQDLLASLQEKKLALHSYRTIAQDVNVHRNILQQLQDRLGTMSDDEATEMLGTVIEAYDKLSGDVEDRINIAEKHVANHEAYLQTFEKTRDWINTIVNEAAPIIEDLTIERDTAKAKIALIGNILQQKAEGDRVLQDCNQQLNIILEQTSITGHPALLKGYEEQKR-------IWDDFLKRCLGTR-------DRLNQLFDQWAEFEKVVERLEAWIKQMESQVKDQSLKSTEETKRAHLQKLKTLEEEIAAKGAEFNAAVEKSQTIEGESE-LGTQ---------------VSRLATKYQAIRNQAKE-------AVARYEQFVREHGVFNEKYNQFLKWITNVQDDLTKHSEIVGDLTVLQSRQKSIRDLGDTRTKENARFESVIDLGEKLY--AHTSPDGR-EIIRQQLRNLRTVWDGFSEDLQNATQKLDQCLMQFAEFSLSQEQLTTWLRDVERAMHQHTELKSSLQEKRAQLQNHKIMHQEIMSHQSLVESVCDKAQQLVDQTKDT---SLNVYLQSIKQLFHNIVAKSQDLLENLEDCVDKHNQFNVQCKNFTDWLNGEREKLLDCNDIAGERSEISRRLATLEALR 3517