BLAST of trna wybutosine-synthesizing protein 1-like protein vs. L. salmonis genes
Match: EMLSAG00000011892 (supercontig:LSalAtl2s:LSalAtl2s838:224621:231489:1 gene:EMLSAG00000011892 transcript:EMLSAT00000011892 description:"maker-LSalAtl2s838-augustus-gene-1.16")

HSP 1 Score: 611.298 bits (1575), Expect = 0.000e+0
Identity = 310/581 (53.36%), Postives = 402/581 (69.19%), Query Frame = 0

Query:    7 YPMVGPLTGPSLAIALLVLALVTWWRRRESARSIRPGVWPQAWSTPVRVPPARPERAQ-------------------PAQKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDF---DEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQH-FNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPSEAGDGLVDLEDIGQVMHRAQVQRAAQAEQGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSKG 564
            + ++GPLT P L +  ++  L+   RRR+S             S P+ + P +  + +                     +K+ ++YG+  G ++ LA  + +  + L+     V+ ++  D+   DE L      G LV+++++TYTEG PP  A++FY    E A+DFR QHSIL+GL++ V+GLGHS YGQ  +N  +RN D++  QLSA  + PL L DEN      G+++ +F  W   L+ ++   + G   E+G         ++   S+   E   E  + +E  DGLVD+ED+G VM +A+  + +     PK M+TP+LR+SLTKQGYK+IG+HSGVKICRWTKAM+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHHSNPVGTEW+W M+ P+ IL+GAL +HY+MIKQMKGVPGVLPE+LAEGM VQHCALSLVGEPIMYPEIN+FV LLH K ISSFLVTNAQFP+AI  + P TQLYVSIDASTK+SLKKIDRPLF+DFWERF+ SL+ELS KGQRTVYRLTLVKG+N EE+ +YAKLV + +PDFIE+KGVTYCGTSK 
Sbjct:    5 WTLIGPLTIPLLIVIAVISTLIL--RRRKS-------------SIPIDISPVKKAKEETQTSCCGSNDCCGNDSSDSSIKKIKVIYGSE-GVSKELALSIKNVASDLS-----VEILSSKDYYPGDEILTDVKETGLLVVVIISTYTEGLPPPDAKYFYSWFEEEAKDFRVQHSILKGLKYCVYGLGHSGYGQEXYNVTSRNLDKFFRQLSAETYLPLSLSDEN-----DGTVQEEFNTWSKALMKKIKGKQNGLISEAGK--------ETIQDSDTDEEYSDEEEEENEEDDGLVDMEDMGVVMKKAKEFKNS----APKDMVTPTLRKSLTKQGYKIIGTHSGVKICRWTKAMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHSNPVGTEWKWSMDPPDMILDGALENHYKMIKQMKGVPGVLPEKLAEGMDVQHCALSLVGEPIMYPEINQFVRLLHSKSISSFLVTNAQFPDAIASMDPCTQLYVSIDASTKESLKKIDRPLFKDFWERFIQSLKELSHKGQRTVYRLTLVKGFNVEELHDYAKLVALGRPDFIEIKGVTYCGTSKA 547          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. L. salmonis genes
Match: EMLSAG00000003122 (supercontig:LSalAtl2s:LSalAtl2s1753:18812:21073:-1 gene:EMLSAG00000003122 transcript:EMLSAT00000003122 description:"maker-LSalAtl2s1753-augustus-gene-0.12")

HSP 1 Score: 78.5666 bits (192), Expect = 1.634e-15
Identity = 54/179 (30.17%), Postives = 88/179 (49.16%), Query Frame = 0

Query:   67 QKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEG------TLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGG 239
            +++++ YG+ TG A   A +LA+       +   V D  + D ++  + E          TL +  +ATY EG P ++A+ F++ L   + +  T       + FAVFGLG+  Y +HFN +    D+ L +L A R H LG GD++       +LE DF  W+    A + + E G G
Sbjct:   99 RRMVVFYGSQTGTAEEFAARLANEGIRYG-LKGVVADPEETDMEDLTKLEEVSDVLDGSPTLAVFCLATYGEGDPTDNAQEFFEWLQTGSCNLST-------MTFAVFGLGNKTY-EHFNAMGIYVDKRLEELGAKRIHKLGKGDDD------DNLENDFVTWKDEFWASVCQ-EFGIG 261          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. SwissProt
Match: gi|123910325|sp|Q2KHP8.1|TYW1_XENLA (RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; AltName: Full=Radical S-adenosyl methionine and flavodoxin domain-containing protein 1; AltName: Full=tRNA wybutosine-synthesizing protein 1 homolog; AltName: Full=tRNA-yW-synthesizing protein)

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 289/537 (53.82%), Postives = 366/537 (68.16%), Query Frame = 0

Query:   69 VLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAW---------------------------DSEASSEASSEADSEAADPSEAGDGLVDLEDIGQVMHRAQVQR----------------AAQAEQGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTS 562
            V I YG+ TG ++  AH LA+ +  L  +  ++ ++ + D D++L  E     + + L+ATYT+G PPESA WF + L EA+ DFR   + L+G+R+AVFGLG+S+Y  H+NTV +N D++L  LSA R      GD NV  S  GS+E+DFE W+ + + RL     G   E  P                                   D+E  +  +S +DSE  +  E G GL+D+ED+G+ M   +  +                 A+ ++ P+ MITP+L+++LTKQGY+LIGSHSGVK+CRWTK+M+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHH+NPVGTEWRW+M+QPE ILE A+ +H  MIKQ KGVPGV PER  EG+ V+HCALSLVGEPIMYPEIN F+ LLH + ISSFLVTNAQFPE IR + PVTQLYVS+DASTKDSLKKIDRPLF+DFW+RF+DSL+ L+ K QRTVYRLTLVK WN +E+  YA LV +  PDFIEVKGVTYCG S
Sbjct:   83 VKIFYGSQTGTSKGFAHLLAEEVTLLG-LPVELINMKEYDPDDNLVEETTSKNICVFLVATYTDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWLWMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKRKFLNRLKALLSG---EKKPCSGKCKKGKCKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFETS-SDSEPEEHGEPGSGLIDVEDLGKAMSNMKKSKREHDSNTELGKSLQGDEAKEKEEPREMITPALQEALTKQGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILEEAILNHQNMIKQFKGVPGVKPERFEEGLAVKHCALSLVGEPIMYPEINTFLRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFIDSLKALAEKQQRTVYRLTLVKAWNVDELKAYADLVALGLPDFIEVKGVTYCGES 614          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. SwissProt
Match: gi|75042502|sp|Q5REF9.1|TYW1_PONAB (RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; AltName: Full=Radical S-adenosyl methionine and flavodoxin domain-containing protein 1; AltName: Full=tRNA wybutosine-synthesizing protein 1 homolog; AltName: Full=tRNA-yW-synthesizing protein)

HSP 1 Score: 576.63 bits (1485), Expect = 0.000e+0
Identity = 285/536 (53.17%), Postives = 362/536 (67.54%), Query Frame = 0

Query:   69 VLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLS-------RPEVGGGLESGPADRTEDAWDSEASSEASSEADS-------------------EAADPSEAGDGLVDLEDIGQVMHRAQ---------------VQRAAQAEQGP-KPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTS 562
            V I YG+ TG A+  A  LA+ +  L ++   + ++ + D D+HL  E     + + L+ATYT+G P ESA WF + L EAA DFR   + L+G+R+AVFGLG+S Y  HFN V +N D++L  L A R    G GD +V  S  GS+EADF  W+ + +++L        +   GG  + G  +  +    SE   E S E D                          ++ + +VD+ED+G++M   +                +   + E G  + MITP+LR++LTKQGY+LIGSHSGVK+CRWTK+M+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHH+NPVGTEWRW+M+QPE IL+ A+ +H  MIKQ KGVPGV  ER  EGM V+HCALSLVGEPIMYPEINRF++LLH+ +ISSFLVTNAQFP  IR++ PVTQLYVS+DASTKDSLKKIDRPLF+DFW RF+DSL+ L+ K QRTVYRLTLVK WN +E+  YA+LV +  PDFIEVKGVTYCG S
Sbjct:   79 VKIFYGSQTGTAKGFATVLAEAVTSL-DLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEAAIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQRG--SEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGKDHQSLNSIVDVEDLGKIMDHVKKEKREKEQREEKSGLFRNMGRNEDGEIRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWRRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGES 611          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. SwissProt
Match: gi|143678193|sp|Q9NV66.2|TYW1_HUMAN (RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; AltName: Full=Radical S-adenosyl methionine and flavodoxin domain-containing protein 1; AltName: Full=tRNA wybutosine-synthesizing protein 1 homolog; AltName: Full=tRNA-yW-synthesizing protein)

HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0
Identity = 284/536 (52.99%), Postives = 364/536 (67.91%), Query Frame = 0

Query:   69 VLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLS-------RPEVGGGLESGPADRTEDAWDSEASSEASSEADS-------------------EAADPSEAGDGLVDLEDIGQVMHRAQ---------------VQRAAQAEQGPK-PMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTS 562
            V I YG+ TG A+  A  LA+ +  L ++   + ++ + D D+HL  E     + + L+ATYT+G P ESA WF + L EA+ DFR   + L+G+R+AVFGLG+S Y  HFN V +N D++L  L A R    G GD +V  S  GS+EADF  W+ + +++L        +   GG  + G  +  +    SE   E S E D                       +  ++ + +VD+ED+G++M   +                +   + E G +  MITP+LR++LTKQGY+LIGSHSGVK+CRWTK+M+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHH+NPVGTEWRW+M+QPE IL+ A+ +H  MIKQ KGVPGV  ER  EGM V+HCALSLVGEPIMYPEINRF++LLH+ +ISSFLVTNAQFP  IR++ PVTQLYVS+DASTKDSLKKIDRPLF+DFW+RF+DSL+ L+ K QRTVYRLTLVK WN +E+  YA+LV +  PDFIEVKGVTYCG S
Sbjct:   79 VKIFYGSQTGTAKGFATVLAEAVTSL-DLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHG--SEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGES 611          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. SwissProt
Match: gi|123913977|sp|Q08C92.1|TYW1_DANRE (RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; AltName: Full=Radical S-adenosyl methionine and flavodoxin domain-containing protein 1; AltName: Full=tRNA wybutosine-synthesizing protein 1 homolog; AltName: Full=tRNA-yW-synthesizing protein)

HSP 1 Score: 571.237 bits (1471), Expect = 0.000e+0
Identity = 280/545 (51.38%), Postives = 369/545 (67.71%), Query Frame = 0

Query:   56 PPARPERAQPAQKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARL------SRPEVGGGLESGPAD----RTEDAWDSEASSEASSEADSEAADPSEAGD---------GLVDLEDIGQVMH-------------------RAQVQRAAQAEQGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTS 562
            P +  ++      V + YG+ TG A+  A +LA+ +     +  +V D+ D D ++ L  E     + + L+ATYT+G P ESA WF + L EA+ DFR   + L+G+R+AVFGLG+S+Y  HFNTV+++ D++L  LSAAR    G GD NV  S  GS++ADF+ W+ + + RL       +    G  +           E+A D+ + +E ++  +      S+             ++D+ED+G VM+                   +   +R ++ E+  + MITP+LR SLTKQGYKLIGSHSGVK+ RWTK+M+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHH+NPVGTEWRW+M+  E+I++ A+ +H  MI+Q +GVPGV PER  EG+ V+HCALSLVGEPIMYPEIN F++LLH++ ISSFLVTNAQFPE IR + PVTQLYVS+DASTKDSLKKIDRPLF+DFW+RF+DSL+ L  K QRTVYRLTLVK WN +E+  YA L+ + QPDFIEVKGVTYCG S
Sbjct:   66 PASEAKKVIYVSGVKVFYGSQTGTAKGFAKELAEDVIAQG-IQCEVIDMKDFDPEDRLAEECTSKIICVFLVATYTDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWLWMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGEKKACSGNCKKASCKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGSVIDMEDLGNVMNHMKKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKLRRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDPAEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKLLHQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLRALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQPDFIEVKGVTYCGES 609          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. SwissProt
Match: gi|81896380|sp|Q8BJM7.1|TYW1_MOUSE (RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; AltName: Full=Radical S-adenosyl methionine and flavodoxin domain-containing protein 1; AltName: Full=tRNA wybutosine-synthesizing protein 1 homolog; AltName: Full=tRNA-yW-synthesizing protein)

HSP 1 Score: 563.533 bits (1451), Expect = 0.000e+0
Identity = 286/555 (51.53%), Postives = 362/555 (65.23%), Query Frame = 0

Query:   54 RVPPARPERAQPAQK------VLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARL------SRPEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPS------------------EAGDGLVDLEDIGQVMHRAQVQRAAQAEQGPKP----------------MITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTS 562
            RVP A      P +K      V I YG+ TG A+  A  LA  +  L ++   + ++ + D D+ L  E    T+   L+ATYT+G P ESA WF + L E+A DFR   + L+GLR+AVFGLG S Y  HFN V+ N D++L  L A R    G GD N   S  GS+EADF  W+ + ++RL       +   GG  + G   + E A      +    + +    D                    ++   +VD+ED+G +M+  + ++  ++ Q  K                 MITP+LR++LTKQGY+LIGSHSGVK+CRWTK+M+RGRGGCYKH+FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEWRW+M+QPE IL+ A+ +H  MIKQ KGVPG+  ER  EGM V+HCALSLVGEPIMYPEINR ++LLH+  ISSFLVTNAQFPE IR ++PVTQLYVS+DAST+D LKKIDRPLF+DFW+RF+DSL+ LS K QRTVYRLTLVK WN +E+  YA+LV +  PDFIEVKGVTYCG S
Sbjct:   50 RVPGAPDSSLSPQKKQTHVSGVKIFYGSQTGTAKGFAVVLAKAVTSL-DLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRG---KCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQSLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTEWRWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEINRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQRFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGES 600          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. SwissProt
Match: gi|75158883|sp|Q8RXN5.1|TYW1_ARATH (RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; AltName: Full=tRNA wybutosine-synthesizing protein 1 homolog)

HSP 1 Score: 494.582 bits (1272), Expect = 5.734e-168
Identity = 260/510 (50.98%), Postives = 328/510 (64.31%), Query Frame = 0

Query:   59 RPERAQPAQKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEH-LRPE-AAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAWDSEASSEASSE---ADSEAADPSEAGDGLVDLEDI-GQVMHRAQVQRAAQAEQGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTS 562
             P     A +  I + + TG A++LA +L +  A          D+A    D H   PE   + TLV+ + +T+  G PP++  +    L E+A+DFR    +L   +FAVFG+G   YG+ +N VA+     +  L      P+G GD +      G L+  F+ W    V R+        L+ G A  T        + E   E   +  E  + ++A  G+VDLEDI G+   +           G K M+TP +R SLTKQGYK+IGSHSGVKICRWTK+ +RGRGGCYKHSFYGI SHRCMETTPSLACANKCVFCWRHH+NPVG  W+W+M++P  I++GAL  H  MIKQMKGVPGV PE+L EG+  +HCALSLVGEPIMYPEIN  V+ LH + IS+FLVTNAQFPE I  + P+TQLYVS+DA+TK+SLK IDRPLF DFWERF+DSL+ L  K QRTVYRLTLVKGWN EE+D Y  L  I +PDFIE+KGVTYCG+S
Sbjct:   40 NPSSTLKASRGKIFFISQTGTAKALAQRLHELCAS--------NDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGESAEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVD-----DGELDRAFQDW-CDGVIRV--------LKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGGIVDLEDIAGKAPSKRNGVVKVTKVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPSVIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSS 527          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. SwissProt
Match: gi|75153207|sp|Q8H8N3.1|TYW1_ORYSJ (RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; AltName: Full=tRNA wybutosine-synthesizing protein 1 homolog)

HSP 1 Score: 489.574 bits (1259), Expect = 6.648e-166
Identity = 261/494 (52.83%), Postives = 334/494 (67.61%), Query Frame = 0

Query:   71 ILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPSE--AGDGLVDLEDIGQVMHRAQVQRAAQAEQGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTS 562
            +LY + TG +++LA  L+ RLA  A V A   D A          +     L++L++ T+  G PP +A +  + L E+A DFR   ++L GLRFAVFG+G   YG+ FN  AR+  R+L  L AA    +G GD +      G LE  FE W  R+V  +   E+G G  +G +D  ++  + E+  +   E         E  AG         G+V    +   +   E G + M+TP +R SL KQGYK+IGSHSGVKICRWTK+ +RGRGGCYKHSFYGI SHRCME TPSLACANKCVFCWRHH+NPVG  W+W+M+ P +I+  A+  H +M+KQMKGVPGV PERLAEG+  +HCALSLVGEPIMYPEIN  ++ LH + IS+FLVTNAQFP+ I+ + P+TQLYVS+DA+TK+SLK +DRPLF DFWERF+DSL+ L  K QRTVYRLTLVKGWNAEE+D YAKL+ + QPDFIE+KGVTYCG+S
Sbjct:   58 LLYASATGTSKALAAGLSRRLAE-AGVTAHPADAA-----AFDPDDLPSLPLLLLVLPTHDGGAPPPAAAFLARWLEESAADFRAGAALLSGLRFAVFGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVD-----GGDLEVVFEEWCGRVVRVVKGEEIGEG-HNGESDGFDELEEEESDDDDDEEEVDGGEVDMEDIAGKAPAARRRNGKV----EGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDELHRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSS 535          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. SwissProt
Match: gi|1375383938|sp|Q6NUM6.4|TYW1B_HUMAN (RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; AltName: Full=Radical S-adenosyl methionine and flavodoxin domain-containing protein 2; AltName: Full=tRNA wybutosine-synthesizing protein 1 homolog B)

HSP 1 Score: 454.521 bits (1168), Expect = 5.027e-152
Identity = 243/524 (46.37%), Postives = 319/524 (60.88%), Query Frame = 0

Query:   81 RSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLS-------RPEVGGGLESGPADRTEDAWDSEASSEASSEADS-------------------EAADPSEAGDGLVDLEDIGQVMHRAQ---------------VQRAAQAEQGPK-PMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTS 562
            +  A  LA+ +  L ++   + ++ + D D+HL  E     + + L+ATYT+G P ESA WF + L EA+ DFR   + L+G+R AVFGLG+S Y  HFN V +N D++L  L   R    G GD +V  S  GS+EA+F  W+ + +++L        +   GG  + G  +  +    SE   E S E D                       +  ++ + +VD+ED+G++M   +                +   + E G +  MITP+LR++LTKQ                     R R     H  +   SHRCMETTPSLACANKCVFCW HH+NPVGTEW W+M+QPE IL+ A+ +H  MIKQ KGVPGV  ER  EGM V+HCALSLVGEPIMYPEINRF++LLH+ +ISSFLVTNAQFP  IR++ PVTQLYVS+DASTKDSLKKIDRPLF+DFW++F+DSL+ L+ K QRTVYRL LVK WN +E+  YA+LV +  PDFIEVKGVTYC  S
Sbjct:   45 QGFATVLAEAVTSL-DLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHG--SEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQ-----------------VDAPRERSLLQTHILWN-ESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKQQRTVYRLMLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCRES 547          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. SwissProt
Match: gi|2501612|sp|Q08960.1|TYW1_YEAST (RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; AltName: Full=tRNA wybutosine-synthesizing protein 1)

HSP 1 Score: 419.468 bits (1077), Expect = 1.107e-136
Identity = 231/510 (45.29%), Postives = 311/510 (60.98%), Query Frame = 0

Query:   66 AQKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQ--VQDVADL-DFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGL-RFAVFGLGHS-LYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPSEAGDGLVDLEDIGQVMHRAQVQRAAQAEQGPKPMI---TPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELS--RKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSKGE 565
            + ++ +LY +  G A   A  + D+L  L E+  +  + ++ DL DFD++      E  L +L++ +Y    P +   +F Q L E A DFR     L+ L  + V GLG S  + + F   A+  D ++ +L   R  PLG     V +   GS  A  + W + L   L               + ++    E    A SE D E  + S   D L D+EDIG      +   A + +Q    M+   +P+ + +LTKQGYK+IGSHSGVKICRWTK  +RG+G CYK S + IAS RCME TPSLAC++KCVFCWRH +NPV   WRW++++PE ILE AL  HY MIKQM+GVPGV+ ER A+   V+HCALSLVGEPI+YP IN+F++LLH+K I+SFLV NAQ PEA+R+I  VTQLYVSIDA TK  LKK+DRPL++DFWER ++ L+ L   +  QRTV+RLTLVKG+N  ++  YA LV    P FIEVKG T+ G+S G 
Sbjct:  202 SSQIYVLYSSLQGAASKAAKSVYDKLKELDELTNEPKLLNLDDLSDFDDYFINVPVENALYVLVLPSYDIDCPLD---YFLQTLEENANDFRVDSFPLRKLVGYTVLGLGDSESWPEKFCYQAKRADHWISRLGGRRIFPLG----KVCMKTGGS--AKIDEWTSLLAETL---------------KDDEPIIYEYDENADSEEDEEEGNGS---DELGDVEDIGGKGSNGKFSGADEIKQ----MVAKDSPTYK-NLTKQGYKVIGSHSGVKICRWTKNELRGKGSCYKKSLFNIASSRCMELTPSLACSSKCVFCWRHGTNPVSKNWRWEVDEPEYILENALKGHYSMIKQMRGVPGVIAERFAKAFEVRHCALSLVGEPILYPHINKFIQLLHQKGITSFLVCNAQHPEALRNIVKVTQLYVSIDAPTKTELKKVDRPLYKDFWERMVECLEILKTVQNHQRTVFRLTLVKGFNMGDVSAYADLVQRGLPGFIEVKGATFSGSSDGN 679          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. SwissProt
Match: gi|74582230|sp|O59761.1|TYW1_SCHPO (RecName: Full=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; AltName: Full=tRNA wybutosine-synthesizing protein 1)

HSP 1 Score: 413.305 bits (1061), Expect = 1.040e-135
Identity = 233/501 (46.51%), Postives = 316/501 (63.07%), Query Frame = 0

Query:   69 VLILYGTTTGRARSLAHQLADRLAHLAEVA-AQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLR-FAVFGLG--HSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPSEAGDGLVDLEDIGQVMHRAQVQRAAQAEQGPKPMIT---PSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTS 562
            + + Y T  G A   A Q+ + L+ L +    Q+ ++  +D  E+      E  + ++++ +Y   +   S  ++  +L E+  DFR     L GL  +AVFGLG   +  G  F   A   D+++ +L A R  PLG+ +  +A + +  ++A  + W  R VA   +   G  L+ G                     DS ++D       ++D+ED+G +M +A+ + A     G K M++   P+ + +LTKQGY ++GSHSGVKICRWTK+ MRGRG CYK+SFYGI SH CME TPSLACANKC FCWRH +NPVGT WRW+++ PE IL+G L +HY  +K MKGVPGVLP+R  E  RV+HCALSLVGEPI YP IN FV +LHE+EISSFLVTNAQ PEA+R++  VTQLYVS+DASTK SLK +DRPLF+DFWER +  L+ L  K QRTVYR+TLVKG+N E++  Y +L+ +  P FIEVKGVTY G S
Sbjct:   91 LCVFYSTLGGTAERYAKQVHEELSSLLQRDDIQLLNLDYVDLSEYF-VSCPENAIYLVVLPSYEIES---SIDYYLSSLQESFSDFRVPKDPLHGLSGYAVFGLGDMENYPGDKFCYQAIQADKWIKKLGARRLAPLGVVNTQLAPTAQ--IDALLQ-W-TRSVAECLKN--GTLLKIG-------------------NTDSLSSD-------VMDVEDMGSMMAKAKAEAALPV--GTKEMVSTESPTYK-ALTKQGYSVVGSHSGVKICRWTKSAMRGRGFCYKYSFYGIRSHLCMEATPSLACANKCTFCWRHGTNPVGTSWRWKVDPPEMILQGILKAHYAKLKLMKGVPGVLPDRYEEASRVRHCALSLVGEPIFYPYINEFVSMLHEREISSFLVTNAQHPEALRNMGMVTQLYVSVDASTKQSLKSVDRPLFKDFWERMLTCLEILREKRQRTVYRMTLVKGFNMEQIKEYTELIRLGVPCFIEVKGVTYSGNS 552          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. nr
Match: gi|1325322500|ref|XP_023343071.1| (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase-like [Eurytemora affinis])

HSP 1 Score: 648.662 bits (1672), Expect = 0.000e+0
Identity = 310/500 (62.00%), Postives = 377/500 (75.40%), Query Frame = 0

Query:   68 KVLILYGTTTGRARSLAHQLADRLAHLAEVAAQV--QDVADLD--FDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPSEAGDGLVDLEDIGQVMHRAQVQRAAQAEQGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSK 563
            KV++LYGTTTG +R  A QL ++L         V  QD A      +E  +  AAEG LVI+L++TYT+GTPPES+ WFY    + A DFR   S+ + L+F +FGLG+SLY ++FN+VA++ D ++H LSA RF PL LGDENV  S  GS+E DF+ W   +  ++SR  +    +  P    ED+  +    E   + D++          LVDLED+G+VM +   ++  ++   PK M+T  LR+SLTKQGYK+IGSHSGVK+CRWTKAM+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHHSNPVGTEWRW+M+  E IL GAL +HY+MI + KGVPGVLPE+LAEGM+ +HCALSLVGEPIMYPEIN+FV LLH K ISSFLVTNAQFP+AIRD+SP TQLYVSIDASTKDSLKKIDRPLF+DFWERFM SL+EL +KGQRTVYRLTLVKGWNAEEMDNYA LV +  PDFIE+KGVT+CG SK
Sbjct:  109 KVVVLYGTTTGNSRKFAEQLTEKLNGKGVTTTPVIHQDCAQYSTMIEEDFKKLAAEGALVIILISTYTDGTPPESSSWFYTWFKDYAHDFRVNRSVFKDLKFCIFGLGNSLYAENFNSVAKDLDSFMHLLSAQRFLPLCLGDENVLESKNGSIEKDFQSWSEEVENKISRSMIEPSTDLSPEVSDEDSISASEGEEEDEDEDADDG--------LVDLEDLGKVMKK---RKNPESVGQPKEMVTDQLRKSLTKQGYKIIGSHSGVKLCRWTKAMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWKMDDAEMILNGALENHYKMINEFKGVPGVLPEKLAEGMQAKHCALSLVGEPIMYPEINKFVTLLHSKGISSFLVTNAQFPDAIRDMSPCTQLYVSIDASTKDSLKKIDRPLFKDFWERFMMSLKELDKKGQRTVYRLTLVKGWNAEEMDNYAALVKLGNPDFIEIKGVTFCGDSK 597          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. nr
Match: gi|1330914153|gb|PNF33337.1| (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cryptotermes secundus])

HSP 1 Score: 627.861 bits (1618), Expect = 0.000e+0
Identity = 291/498 (58.43%), Postives = 380/498 (76.31%), Query Frame = 0

Query:   67 QKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPSEAGDGLVDLEDIGQVMHRAQVQRAAQAE-QGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSK 563
            + V + +GT TG+++  +  LA+       +++++ D+A  D +E L  E +  +L + ++ TYTEG PPESA WFY+ L EA+ DFR +  +L+G++FAVFGLG+S Y  HFN V +  D +L +L AAR   LG+GDE+   S  GS+EADF++W ++L AR+ +       ++   D+ E + DS  SSE    +D ++++    G+G++DLE++G   +     R  + E   P+ M+T  L++SLTKQGY+++G+HSGVK+CRWTK+M+RGRGGCYKHSFYGI SHRCMETTPSLACANKCVFCWRHH+NPVGTEWRW+M+ PE+I+  AL +HY MI+Q +GVPGVLPER AEGM V+HCALSLVGEPIMYPEI  FV LLHE+ IS+FLVTNAQFPEA+RD+SPVTQLYVS+DASTK SL+KIDRPLF+DFWERF+DSL+ LS KGQRTVYRLTLVK WNA+E+ +YAKLV I +PDFIEVKGVTYCGTSK
Sbjct:  186 ETVKVFFGTQTGKSKIFSTVLAEGCEQRG-LSSELVDLASADPEERLLEETSGNSLCVFILPTYTEGKPPESAAWFYKWLQEASNDFRVEKELLKGMKFAVFGLGNSQYKAHFNEVGKQVDGWLQELHAARLIKLGIGDEDTVASKHGSIEADFQNWNSQLWARVDKLRAACQCDATEQDQAESSCDS--SSEGCY-SDGDSSEQDSRGNGVLDLEELGSFSNSLLAARERKFEDNSPREMVTALLKESLTKQGYRIVGTHSGVKLCRWTKSMLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDPPEQIMTEALENHYNMIRQFRGVPGVLPERFAEGMDVKHCALSLVGEPIMYPEIGSFVRLLHERGISTFLVTNAQFPEALRDLSPVTQLYVSVDASTKSSLRKIDRPLFKDFWERFIDSLKALSEKGQRTVYRLTLVKSWNADEIADYAKLVAIGKPDFIEVKGVTYCGTSK 679          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. nr
Match: gi|1339093279|ref|XP_023707871.1| (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase-like [Cryptotermes secundus] >gi|1330914152|gb|PNF33336.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cryptotermes secundus])

HSP 1 Score: 625.935 bits (1613), Expect = 0.000e+0
Identity = 291/498 (58.43%), Postives = 380/498 (76.31%), Query Frame = 0

Query:   67 QKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPSEAGDGLVDLEDIGQVMHRAQVQRAAQAE-QGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSK 563
            + V + +GT TG+++  +  LA+       +++++ D+A  D +E L  E +  +L + ++ TYTEG PPESA WFY+ L EA+ DFR +  +L+G++FAVFGLG+S Y  HFN V +  D +L +L AAR   LG+GDE+   S  GS+EADF++W ++L AR+ +       ++   D+ E + DS  SSE    +D ++++    G+G++DLE++G   +     R  + E   P+ M+T  L++SLTKQGY+++G+HSGVK+CRWTK+M+RGRGGCYKHSFYGI SHRCMETTPSLACANKCVFCWRHH+NPVGTEWRW+M+ PE+I+  AL +HY MI+Q +GVPGVLPER AEGM V+HCALSLVGEPIMYPEI  FV LLHE+ IS+FLVTNAQFPEA+RD+SPVTQLYVS+DASTK SL+KIDRPLF+DFWERF+DSL+ LS KGQRTVYRLTLVK WNA+E+ +YAKLV I +PDFIEVKGVTYCGTSK
Sbjct:  186 ETVKVFFGTQTGKSKIFSTVLAEGCEQRG-LSSELVDLASADPEERLLEETSGNSLCVFILPTYTEGKPPESAAWFYKWLQEASNDFRVEKELLKGMKFAVFGLGNSQYKAHFNEVGKQVDGWLQELHAARLIKLGIGDEDTVASKHGSIEADFQNWNSQLWARVDKLRAACQCDATEQDQAESSCDS--SSEGCY-SDGDSSEQDSRGNGVLDLEELGSFSNSLLAARERKFEDNSPREMVTALLKESLTKQGYRIVGTHSGVKLCRWTKSMLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDPPEQIMTEALENHYNMIRQFRGVPGVLPERFAEGMDVKHCALSLVGEPIMYPEIGSFVRLLHERGISTFLVTNAQFPEALRDLSPVTQLYVSVDASTKSSLRKIDRPLFKDFWERFIDSLKALSEKGQRTVYRLTLVKSWNADEIADYAKLVAIGKPDFIEVKGVTYCGTSK 679          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. nr
Match: gi|1228019555|ref|XP_021941762.1| (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase-like [Zootermopsis nevadensis] >gi|646676375|gb|KDQ97265.1| TRNA wybutosine-synthesizing protein 1-like protein [Zootermopsis nevadensis])

HSP 1 Score: 619.772 bits (1597), Expect = 0.000e+0
Identity = 285/496 (57.46%), Postives = 373/496 (75.20%), Query Frame = 0

Query:   69 VLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPSEAGDGLVDLEDIGQVMHRAQVQRAAQAEQG-PKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSK 563
            V + +GT TG++++ + +L  R      ++ ++ D+A  D +E L  E +  +L + ++ T+T+G PPESA WF + L EA+ DFR +  + +G++FAVFGLG+S Y  HFN V +  DR+L  L AARF  LG+GDE+ + S RGS+EADF+ W  +L  R+ R       +    ++ E + DS   S    ++D  ++     G G++DLE++G      +  R  + E+  P+ M+T +LR SLT+QGY+++G+HSGVK+CRWTK+M+RGRGGCYKHSFYGI SHRCMETTPSLACANKCVFCWRHHSNPVGTEWRW+M+ P++IL+ +L +HY MIKQ KGVPGVLPER AEGM V+HCALSLVGEPIMYPEI+ FV LLHE  IS+FLVTNAQFP+A+RD+ PVTQLYVS+DASTK SL+KIDRPLF+DFWERF+DSL+ LS KGQRTVYRLTLVK WN +E+ +Y+KLV+I +PDFIEVKGVTYCGTSK
Sbjct:  157 VKVFFGTQTGKSKTFSTKLG-RCCEQIGLSVELVDLASFDPEERLLEETSGKSLCVFILPTHTDGKPPESAAWFCKWLEEASNDFRVERELFKGMKFAVFGLGNSQYKDHFNQVGKQVDRWLQDLHAARFIKLGVGDEDTSASKRGSIEADFQDWIDQLWMRVDRFRTACQCDGSEQEKAESSCDS---SSEGCDSDGNSSGQDSHGTGVIDLEELGAFSSSFRAAREKEVEENSPREMVTAALRDSLTRQGYRIVGTHSGVKLCRWTKSMLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWKMDPPDKILKDSLENHYNMIKQFKGVPGVLPERFAEGMDVKHCALSLVGEPIMYPEISNFVLLLHEHGISTFLVTNAQFPKALRDMGPVTQLYVSVDASTKSSLRKIDRPLFKDFWERFIDSLKALSEKGQRTVYRLTLVKSWNVDEIMDYSKLVEIGKPDFIEVKGVTYCGTSK 648          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. nr
Match: gi|1022761065|gb|KZS07103.1| (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Daphnia magna])

HSP 1 Score: 619.002 bits (1595), Expect = 0.000e+0
Identity = 307/607 (50.58%), Postives = 407/607 (67.05%), Query Frame = 0

Query:    8 PMVGPLTGPSLAIALLVLALVTWWRRRESARSIRP---------GVWPQAWSTPVRVPPARPERAQPAQ--------------KVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQ--DVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPAD------------------RTEDAWDSEASSEASSEADSEAADPSEAGDGLVDLEDIGQ--------VMHRAQVQRAAQAEQGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSK 563
            P++G    PSL +A+L L +V +W  R+   +I+          G+  + +S P    P R ++                   ++ I+YGT  G+++  A  L   L H+AE   +V   ++ + D +++     A+ TL I +++TY EG+PP+ A+WFY+ L ++A DFR   ++L  +RF+VFGLG+SLY +++N + RN D +L QL A R+ PLGLGDENVA S+ G++EADF+HW   ++  L +P+     +SG +                   + ++    EA     +++DS++ +P      ++DLED+G         V    + +         + M+TP LR+SLTKQGY+LIG+HSGVK+CRWTKAM+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHHSNPVGTEWRW+M+  E ILEGAL  H +MI Q KGVPGV P RL EGM   HCALSLVGEPIMYPEINRF+ +LHEK+ISSFLVTNAQFPEAIR + P TQLYVS+DASTKDSLK+IDRPLF+DFWERF+DSL+ LS KGQRTVYRLTLVK WN EE+++YA LV + +PDFIE+KGVT+CGTSK
Sbjct:    8 PLIGSFFLPSLGLAVLSL-IVGYWVTRKKKDNIKSELDINESPSGLENELYSMPATENPKRKKKKHVCNCGSTQASNFEKHPSRIKIMYGTLMGKSKKFAEML---LGHVAEDGMEVSAINLKECDSEDNFVGSDAKDTLFIFIVSTYAEGSPPDDAQWFYKWLEDSAFDFRVPKTLLADVRFSVFGLGNSLYSENYNVIGRNIDNWLSQLGAERYAPLGLGDENVAESLHGNIEADFDHWSEGILNLLKQPQHTTVNKSGCSKGNDCCSKKGKKKITNKFQKKKNNILEEADVTYDTDSDSDSNEP------VLDLEDLGSFVRVENKAVEDSEEEEEINVENGETREMLTPQLRESLTKQGYRLIGTHSGVKLCRWTKAMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWKMDPAELILEGALEQHTKMINQFKGVPGVQPNRLVEGMSPVHCALSLVGEPIMYPEINRFLRMLHEKKISSFLVTNAQFPEAIRQLEPCTQLYVSVDASTKDSLKRIDRPLFKDFWERFLDSLRALSAKGQRTVYRLTLVKAWNVEELESYANLVKLGEPDFIEIKGVTFCGTSK 604          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. nr
Match: gi|1316141448|ref|XP_023215687.1| (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase-like isoform X1 [Centruroides sculpturatus])

HSP 1 Score: 616.305 bits (1588), Expect = 0.000e+0
Identity = 287/502 (57.17%), Postives = 369/502 (73.51%), Query Frame = 0

Query:   67 QKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPSEAGDGLVDLEDIGQVMHRAQVQRAAQA----EQGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSKG 564
            + V + YGT TG A+ L+ +L + L        ++ ++ D D D+    EA + T  I +++TY +G PP+ A+WF + L +   DFR Q  IL G+++ VFGLG+SLYG++FN V++  D +L++L A R   +GLGDEN+A S  G +  DF  W+ RL+  L +PE G   +   ADR     + E   E+S   D +  D    G  LVD+ED+G VM++ +  + A+     + GPK MITP+LR++LTKQGYKLIGSHSGVK+CRWTK+M+RGRGGCYKH+FYGI SHRC+E TPSLACANKCVFCWRHHSNPVGTEW+W+M+ PE I + AL +HY+MIK+ KGVPGV  ER  EGM  +HCALSLVGEPIMYPEIN+FV LLH + IS+FLVTNAQFP+AI  + PVTQLYVS+DA++KDSLKKIDRPLFRDFW RF+DSL+ LS KGQRTVYRLTLVK WN EE++ YAKL+++ +PDFIE+KGVTYCGTSK 
Sbjct:   82 KNVKVFYGTQTGNAKKLSKELVETLQDKG-WTVELINLKDYDPDDSFLEEAKKKTTCIFIISTYVDGHPPDDAKWFCKWLKDICFDFRMQKFILNGMKYGVFGLGNSLYGENFNKVSKELDEWLNKLEAERIMDVGLGDENMAESKNGDIHLDFVEWKKRLIEILEKPERGYSSQQIKADR-----NKEMLYESS---DDDVEDVGSDGSELVDVEDLGSVMNKMKNIKVAKKKILEDSGPKEMITPTLRKALTKQGYKLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCVEATPSLACANKCVFCWRHHSNPVGTEWKWKMDNPELIFQEALKNHYRMIKEFKGVPGVKEERFKEGMEAKHCALSLVGEPIMYPEINKFVRLLHGRGISTFLVTNAQFPDAISKLDPVTQLYVSVDAASKDSLKKIDRPLFRDFWTRFLDSLKALSDKGQRTVYRLTLVKAWNTEEIEGYAKLIELGRPDFIEIKGVTYCGTSKA 574          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. nr
Match: gi|1229165437|ref|XP_022095801.1| (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase-like [Acanthaster planci])

HSP 1 Score: 609.757 bits (1571), Expect = 0.000e+0
Identity = 308/530 (58.11%), Postives = 380/530 (71.70%), Query Frame = 0

Query:   63 AQPAQKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRP------------EVG---GGLESGPADRTEDAWDSEASSEASSEADSEAADPSEAG-----------DGLVDLEDIGQVMHRAQVQRAAQAEQG---PKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSK 563
            + P  ++ ILYGT TG A+  A  LA ++A     AA+V D+   D + H+ PE       +L++ATY+EG P ESA WF + L EA  DFR   S+L+GL++AVFGLG+SLY  H+N VAR+ DR+L  LSA+     G GD+NVA S  G +EADFE W+  L+  L+              E G   GG        + +A D E   ++SSE D+   D  + G           +GL DLED+G+VM + +  + ++A      P+ MITP LRQ+L+KQGY+LIGSHSGVK+CRWTK+M+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHH+NPVGTEWRW+M++PE IL+ AL +HYQ+IKQ KGVPGV PER+AE M VQHCALSLVGEPIMYPEINRF +LLH  +IS+FLVTNAQFP+AIR + PVTQLYVS+DASTK+SLKKIDRPLFRDFW RF+ SL+ LS KGQRTVYRLT+V GWN EE++ YA LV I +PDFIEVKGVTYCG SK
Sbjct:  147 SSPKHRLRILYGTQTGTAKHFAEDLAKQVASTGN-AAEVVDLKTFDPESHILPETDGLVTCVLIVATYSEGKPTESAAWFCRWLEEATTDFRVHKSLLRGLKYAVFGLGNSLYEGHYNQVARDVDRWLSTLSASAIVARGEGDQNVAESKHGGIEADFEAWKTLLLQTLASNASICCSTEKQTCECGKTEGGCRQATEKESRNA-DKEEMYDSSSEDDNCDDDFIDDGSGDAKSKITQTNGLTDLEDLGKVMKKMKKAKESKASDKASEPREMITPMLRQALSKQGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDEPEAILKDALKNHYQLIKQFKGVPGVKPERMAEAMNVQHCALSLVGEPIMYPEINRFAKLLHSHKISTFLVTNAQFPDAIRSMVPVTQLYVSVDASTKESLKKIDRPLFRDFWPRFLSSLEALSEKGQRTVYRLTIVNGWNNEEIEAYANLVSIGKPDFIEVKGVTYCGESK 674          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. nr
Match: gi|1242740128|ref|XP_022329631.1| (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase-like [Crassostrea virginica])

HSP 1 Score: 609.372 bits (1570), Expect = 0.000e+0
Identity = 294/506 (58.10%), Postives = 373/506 (73.72%), Query Frame = 0

Query:   67 QKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARL-SRPEVGG------GLESGPADRTEDAWDSEASSEASSEADSEAADPSEAGDGLVDLEDIGQVM-HRAQVQRAAQAEQGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSKG 564
            +K+ ILYGT TG+++  A  L+ + A+      +V D+ + D DE L  E+     +++ ++TYTEGT P+ A WF Q L E+A DFR Q S+L  L +AVFGLG+SLY +HFNTV ++ D YLH+LS  R  P+GLGDENV+ S +G +E D+  W+     +L ++  V          E+G     E+  D   S+ + SE+D++ +     GD +VD+EDIG+VM  + Q  +   + +    MITP +++SL KQGYKLIGSHSGVK+CRWTK+M+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHH+NPVGTEWRW+M+  E +  GAL +H  +IKQ KGVPGV PER  EG+  +HCALSLVGEPIMYP+IN+F+ELLH+KEIS+F+VTNAQFP+AIRD+SPVTQLYVS+DASTKDSLKKIDRPLFRDFWERF+ SL  LS K QRTVYRLTLVK WN +E+DNYA LV   +PDFIE+KGVTYCG SK 
Sbjct:   76 KKIKILYGTQTGKSKYFADNLS-KDANYKGYQCEVVDLQNYDPDESLSNESNNAVCLVIFISTYTEGTAPKGAEWFVQWLEESASDFRVQKSLLSNLHYAVFGLGNSLYEEHFNTVGKHVDHYLHKLSGHRILPVGLGDENVSQSHKG-VENDYVAWKREFWKKLKNKASVSAPKGNEKTCENGGQCTCEEKDDVIDSNSSESESDNDESTEDVTGDDIVDVEDIGRVMGSKKQATKVESSNKELSEMITPIIKKSLEKQGYKLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDDAEFVFNGALQNHVSLIKQFKGVPGVQPERFLEGLTPKHCALSLVGEPIMYPKINKFIELLHDKEISTFMVTNAQFPDAIRDLSPVTQLYVSVDASTKDSLKKIDRPLFRDFWERFISSLDALSAKCQRTVYRLTLVKAWNTDELDNYASLVSRGKPDFIEIKGVTYCGESKA 579          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. nr
Match: gi|321479494|gb|EFX90450.1| (hypothetical protein DAPPUDRAFT_189816 [Daphnia pulex])

HSP 1 Score: 607.831 bits (1566), Expect = 0.000e+0
Identity = 305/608 (50.16%), Postives = 408/608 (67.11%), Query Frame = 0

Query:    8 PMVGPLTGPSLAIALLVLALVTWWRRRE--SARSIRPGVWPQAWSTPVRVPPARPE-----------------------RAQPAQKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSRPEVGGGLESG---------------PADRTEDAWDSEAS-SEASSEADSEAADPSEAGDGLVDLEDIGQVMHRAQVQRAAQAEQG---------PKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKG-VTYCGTSKG 564
            P++G    P+L +A+  LAL  W  RR+  +  +  P V  ++    V    +                            QP+Q + I+YGT  G+++  A  L++ ++   E    V ++ + D ++       + TL I +++TY EG+PPE A WFY+ L ++A DFR   + L  +RF+VFGLG+SLY +++N + RN D++L ++ A R+ PLGLGDENV+ S+ G++EADF+HW   ++  L  P+     +SG                 +R++   ++    ++ + ++DSE    S+  + +VDLED+G  + R + +    +E+          P+ M+TP LR+SLTKQGY+LIG+HSGVK+CRWTKAM+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHHSNPVGTEWRW+M+  E ILEGAL  H +MI Q KGVPGV P RLAEGM   HCALSLVGEPIMYPEINRF+ +LHEK+ISSF+VTNAQFPEAIR + P TQLYVS+DASTKDSLKKIDRPLF+DFWERF+DSL+ LS KGQRTVYRLTLVK WN EE+++YA+LV + +PDFIE+KG VT+CGTSK 
Sbjct:    7 PLIGSFFLPTLGLAVFSLALGYWISRRKKGNINNDEPNVTEKSSDDTVGETDSMSALDNLKKKKKKHDCNCGSKETSKFEKQPSQ-IKIMYGTLMGKSKKFAEILSEHVSE--ETPVSVVNLKECDSEDIFVGPDTKDTLFIFIVSTYAEGSPPEDAEWFYKWLEDSALDFRVPQTFLAEVRFSVFGLGNSLYFENYNVIGRNIDKWLSKIGAERYAPLGLGDENVSESLHGNIEADFDHWCEGILNLLKNPQGTTEKKSGCSKGNDCCSKKGKKKSVNRSQKKKNNIVEETDVTRDSDSE----SDTNEAVVDLEDLGSFV-RVENKAGEDSEEDEEVTTVDGEPREMLTPQLRESLTKQGYRLIGTHSGVKLCRWTKAMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWKMDPAEFILEGALEQHAKMINQFKGVPGVQPHRLAEGMNPVHCALSLVGEPIMYPEINRFMRMLHEKKISSFMVTNAQFPEAIRQLEPCTQLYVSVDASTKDSLKKIDRPLFKDFWERFLDSLRALSAKGQRTVYRLTLVKAWNVEELESYAELVKLGEPDFIEIKGSVTFCGTSKA 606          

BLAST of trna wybutosine-synthesizing protein 1-like protein vs. nr
Match: gi|762099207|ref|XP_011432019.1| (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Crassostrea gigas])

HSP 1 Score: 607.06 bits (1564), Expect = 0.000e+0
Identity = 290/502 (57.77%), Postives = 361/502 (71.91%), Query Frame = 0

Query:   67 QKVLILYGTTTGRARSLAHQLADRLAHLAEVAAQVQDVADLDFDEHLRPEAAEGTLVILLMATYTEGTPPESARWFYQALAEAAQDFRTQHSILQGLRFAVFGLGHSLYGQHFNTVARNCDRYLHQLSAARFHPLGLGDENVAVSVRGSLEADFEHWQARLVARLSR-PEVGGGLESGPADRTEDAWDSEASSEASSEADSEAADPSE---AGDGLVDLEDIGQVMHRAQVQ-RAAQAEQGPKPMITPSLRQSLTKQGYKLIGSHSGVKICRWTKAMMRGRGGCYKHSFYGIASHRCMETTPSLACANKCVFCWRHHSNPVGTEWRWQMNQPEEILEGALTSHYQMIKQMKGVPGVLPERLAEGMRVQHCALSLVGEPIMYPEINRFVELLHEKEISSFLVTNAQFPEAIRDISPVTQLYVSIDASTKDSLKKIDRPLFRDFWERFMDSLQELSRKGQRTVYRLTLVKGWNAEEMDNYAKLVDIAQPDFIEVKGVTYCGTSK 563
             K+ ILYGT TG+++  A  L+    +      +V D+ + D DE L  E    T +++ ++TYTEGTPPE A WF + L E+A DFR Q S+L  L +AVFGLG+SLYG++FNTVA++ D  LH+LS  R   +GLGDENV+ S   SLE D+  W+     +L     V    +     + E   + +    AS     E+ D  +   A + +VD+ED+G+VM   +   +    +  P  MITP +++SL KQGYKLIG+HSGVK+CRWTK+M+RGRGGCYKH+FYGI SHRCMETTPSLACANKCVFCWRHH+NPVGTEWRW+M+  E +  GAL SH  +IKQ KGVPGV PER  EG+  QHCALSLVGEPIMYP+IN+F++LLH+K IS+F+VTNAQFP+AIRD+SPVTQLYVS+DASTKDSLKKIDRPLFRDFW+RF++SL  LS K QRTVYRLTLVK WN EE+DNYA LV    PDFIEVKGVTYCG SK
Sbjct:   72 NKIKILYGTQTGKSKHFAENLSKDGTYKG-YTCEVIDLKNYDPDEQLDNEGENTTCLVVFISTYTEGTPPEGAEWFVKWLEESASDFRVQKSLLSNLHYAVFGLGNSLYGENFNTVAKHVDHCLHKLSGHRILSVGLGDENVSQSSHKSLENDYVAWKRDFWKKLKEVNSVFASKDLKKNCKNEGQCNCQEEDTASENTGIESDDELQEDPADNEIVDVEDLGRVMGSGKTSTKLNPTKDEPAEMITPLIKKSLEKQGYKLIGTHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDDAEFVFNGALQSHVALIKQFKGVPGVQPERFLEGLTPQHCALSLVGEPIMYPKINKFIDLLHDKGISTFMVTNAQFPDAIRDLSPVTQLYVSVDASTKDSLKKIDRPLFRDFWDRFLESLDALSAKNQRTVYRLTLVKAWNTEELDNYASLVSRGNPDFIEVKGVTYCGESK 572