BLAST of pyruvate carboxylase vs. L. salmonis genes
Match: EMLSAG00000007092 (supercontig:LSalAtl2s:LSalAtl2s3:857289:864181:1 gene:EMLSAG00000007092 transcript:EMLSAT00000007092 description:"maker-LSalAtl2s3-snap-gene-8.38")

HSP 1 Score: 424.091 bits (1089), Expect = 1.417e-134
Identity = 214/473 (45.24%), Postives = 304/473 (64.27%), Query Frame = 0

Query:  365 VTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAV-NIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQG---HTRGNNTDITKLNEFSNYWEDIREFYYPFESE--LKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            + ISPF  + F     +++S+E WGGATFDV MRFL E PW RL+ +R  +PNI  QMLLR +NAVGYT+YPDN++ KF   A + GMD+FRIFDSLN+  ++ L +  V  K   + E  I YTGD+ +P+K K++L+YY+ LA EL + G H+LCIKDMAGLL P AA  LI AL++   +IPIH+HTHDTS    A+ +   +AG D++DVA+ SMSG+TSQP+  +    L G     +G   D   ++ +S++WE  R  Y PFE    +++G++ V+E+EIPGGQY+NL+ QA  LG+ D+F  +K+ Y  AN++ G +VKVTPSSK+VGD+A FM  N  +++++  +  + +FP SVI F +G IGQPYGGFP   +  ++K       RP   L P+DF+    + + +FG+  ++LD +S  +YPKV +DF  F    G V    T  F  G K  EE  + I
Sbjct:  386 LKISPFVAQKFPN---QLYSLENWGGATFDVSMRFLHECPWERLQQMRALVPNIPFQMLLRGANAVGYTSYPDNVIYKFCDLAVKTGMDVFRIFDSLNYLPNLILGVDAV-GKAGGVIEGAISYTGDVTDPSKTKYDLKYYLKLADELVKAGIHVLCIKDMAGLLKPQAATILIDALRQKYPDIPIHIHTHDTSGAGVASMINCAKAGADIVDVAVDSMSGMTSQPSMGAFVASLTGSPQEVKGAVMD--DISRYSSFWEQTRTLYGPFECTKTMRSGSSDVFEHEIPGGQYTNLQFQAFSLGLGDQFMDVKKKYAQANQILGDIVKVTPSSKIVGDLAQFMVQNNLSRKEVEERASELSFPQSVIEFLQGYIGQPYGGFPEPFRSNVIKGGPVVEGRPGVELDPIDFDDLQEKLKLKFGDGISELDVMSAAMYPKVAEDFFTFRNQYGPVDKFNTRHFLIGPKIAEEFEVTI 852          

HSP 2 Score: 253.062 bits (645), Expect = 1.376e-71
Identity = 130/271 (47.97%), Postives = 182/271 (67.16%), Query Frame = 0

Query:  150 MRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLD-AEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNIL 419
            MRVV   E++   F  A +EA KAFG  +IF+E+++++P+HIEVQ++GD++GN VHL+ERDCSVQRR QKVVE+APAP+L    + K+ + A+ + K VGY NAGTVEFL++ +    +F+EVN R+QVEHT+TEEIT +D+V+TQI +  G  L   ++    QE +   G+AIQCR+TTEDP  NFQPD G I  +R+  GMGIRLD  S++ G  ISP++D    K      ++E    AT     R LKE    R+R ++  +P +L
Sbjct:    1 MRVVTKLEDVPGQFERAHSEAIKAFGNGSIFIERYVQHPRHIEVQIMGDKYGNTVHLFERDCSVQRRHQKVVEIAPAPNLDPVIRQKILDDAVHLCKHVGYSNAGTVEFLVEPSTGQHFFMEVNARLQVEHTVTEEITCVDLVQTQIKVEEGHSLPDLNL---NQESIQHHGYAIQCRLTTEDPAKNFQPDTGRIEVFRSGEGMGIRLDSASAFAGAIISPYYDSLLVKVIARASTLE----ATCSKLSRSLKE---FRIRGVKTNIPFVL 261          

BLAST of pyruvate carboxylase vs. L. salmonis genes
Match: EMLSAG00000010863 (supercontig:LSalAtl2s:LSalAtl2s721:350793:353204:-1 gene:EMLSAG00000010863 transcript:EMLSAT00000010863 description:"maker-LSalAtl2s721-augustus-gene-2.49")

HSP 1 Score: 308.145 bits (788), Expect = 3.102e-94
Identity = 167/401 (41.65%), Postives = 246/401 (61.35%), Query Frame = 0

Query:    2 ATELKIRTIALYTFEDRYSLHRYKADEAYQIGE---------DEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASG--LPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTI--IAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWG 389
            A  + I T+A+Y+  D+ S+H   AD+AY IG           ++ ++ YL  ++II +A+K  A AIHPGYGFLSENVEFA+ C+++GI F+GP   ++  +G K  +KII   ANVP I     +        SEA  IG+PIM+KA  GGGG+GMR+  TE+E  +    AK E  K+FG + + LEK+++NP+H+EVQ+ GD+HGN V+L+ERDCSVQRR QK++E AP P +  E + KL E  ++  + V Y  AGTVEF++D + N YF+E+N R+QVEH +TE ITG D+V+ Q+  A+G  LP+         Q+D+ + G++ + RI  EDP  +F P  G +  ++Y  A    +R++ G    G  +S  +D   AK       + +WG
Sbjct:   36 ANSMGIETVAVYSDADKGSMHVEMADQAYYIGPPASQQSYLVQDKIIESYLVQDKIIEVAQKSGAQAIHPGYGFLSENVEFAELCQKKGIIFVGPPASAIRDMGIKSTSKIIMTEANVPCIGGYHGEDQSFERLKSEASKIGYPIMIKAVRGGGGKGMRIALTEDEFEQQLNSAKTEGLKSFGDEVMLLEKYVQNPRHVEVQVFGDQHGNYVYLFERDCSVQRRHQKIIEEAPGPFISWEVRRKLGEAXVRAARAVXYVGAGTVEFIMDKDFNFYFMEMNXRLQVEHPVTEMITGTDLVKWQLQAAAGQVLPIT--------QDDIKLNGWSFEARIYAEDPDQDFMPGAGPLHHVSYPKADD-NVRIETGIRQ-GDEVSVHYDPMIAK-------LVVWG 419          

BLAST of pyruvate carboxylase vs. L. salmonis genes
Match: EMLSAG00000011449 (supercontig:LSalAtl2s:LSalAtl2s790:139998:142185:-1 gene:EMLSAG00000011449 transcript:EMLSAT00000011449 description:"maker-LSalAtl2s790-augustus-gene-1.35")

HSP 1 Score: 291.197 bits (744), Expect = 2.928e-87
Identity = 165/379 (43.54%), Postives = 240/379 (63.32%), Query Frame = 0

Query:    2 ATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPL-KPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNF-QPDYGTIIAYRNAAGM-GIRLDEGSSYPGVTISPFFDKSFAK 377
            A ++ I+T+A+++  D  SL    ADE   IG    P+ + YL  E+II   K   A A+HPGYGFLSEN  F  +  E G+KFIGP+ E++  +GDK+A+K +A  A V  I     ++      L  A  I +P+M+KA++GGGG+GMRV   +E+  +A+   K E K  FG D + +EKF+++P+H+E+QLLGD+HGN ++L ER+CS+QRR QKV+E AP+P L + T+ K+ E A+ + K +GY +AGTVEFL+D+++N YF+E+N R+QVEH ITE ITG+D+V+  I  + G PL        KQED+ I G+AI+ R+  EDP  +F  P  G +  Y   + + G+R D G    G  IS F+D    K
Sbjct:   60 AKKMGIKTVAVHSVADGGSLFVKMADETINIGP--PPVNESYLVQEKIIDAVKLTGAQAVHPGYGFLSENTSFVAKLEELGVKFIGPNSEAIKAMGDKIASKRVATDAKVNGIPGFDGEIENEEHCLHLANEIKYPVMIKASSGGGGKGMRVSWNDEQTREAYRLCKEEGKNFFGDDRLLIEKFVDSPRHVEIQLLGDKHGNAIYLNERECSIQRRNQKVIEEAPSPFLDETTRKKMGEQAVSLAKTIGYDSAGTVEFLMDSQKNFYFLEMNTRLQVEHPITECITGVDLVQQMIRTSYGHPLN------LKQEDIKIDGWAIESRVYAEDPFKDFGMPSIGRLYQYEEPSYLPGVRCDSGIE-EGSEISMFYDPMICK 429          

BLAST of pyruvate carboxylase vs. L. salmonis genes
Match: EMLSAG00000008225 (supercontig:LSalAtl2s:LSalAtl2s496:326845:336125:1 gene:EMLSAG00000008225 transcript:EMLSAT00000008225 description:"maker-LSalAtl2s496-snap-gene-3.19")

HSP 1 Score: 133.65 bits (335), Expect = 5.395e-32
Identity = 75/223 (33.63%), Postives = 119/223 (53.36%), Query Frame = 0

Query:  138 MVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITK--------QEDVNIQ---------GFAIQCRITTEDPQNNFQPDYGTI 343
            M+KA+ GGGG+G+R   ++EE  K + + + E   +     IF+ K  +N +H+EVQLL DE+G  + L+ RDCS+QRR QK++E AP          ++ E A++  + VG                 YF+E+NPR+QVEH  TE +  I++  +Q+++A G+PL H    I +         E +N +         G  I  R+T+E+P   F+P  GT+
Sbjct:    1 MIKASEGGGGKGIRKAASDEEFRKQYPQVQAEVPGS----PIFVMKLAQNARHLEVQLLADEYGTAISLFGRDCSIQRRHQKIIEEAPVIVASPNVFREMEEAAVRXAELVG-----------------YFLELNPRLQVEHPCTEMVANINLPASQLMVAMGIPL-HRMKSIRRLYSQDSLETESINFKCPNPRPCHNGHVIAARVTSENPDEGFKPSSGTV 201          

BLAST of pyruvate carboxylase vs. L. salmonis genes
Match: EMLSAG00000008174 (supercontig:LSalAtl2s:LSalAtl2s490:346891:359146:1 gene:EMLSAG00000008174 transcript:EMLSAT00000008174 description:"maker-LSalAtl2s490-snap-gene-3.20")

HSP 1 Score: 64.6994 bits (156), Expect = 1.059e-10
Identity = 57/227 (25.11%), Postives = 104/227 (45.81%), Query Frame = 0

Query:   90 ESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQR------RFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDA-EENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIF 309
            E +A++G+KVA   I     V  +E+          A+  AK +G+P++ +AA   GG G      EEEL K    +   + +      + L+K ++  K IE +++ D + N + +    C+++          + + VAP+ +L     + L   +IK+ + +G      +++ LD   E  Y IEVN R+     +  + TG  +      +A G+PL  PS+ 
Sbjct:  482 EKIAEIGEKVAPSKI-----VSCMEE----------AVQSAKELGYPVLARAAYALGGLGSGFADNEEELRKLTPSSFAHSSQ------VILDKSLKGWKEIEYEVVRDAYDNCITV----CNMENLDPLGIHTGESIVVAPSQTLTDREYNFLRTTSIKVIRHLGVVGECNIQYALDPNSEKYYIIEVNARLSRSSALASKATGYPLAYIAAKLAMGVPL--PSLI 681          

BLAST of pyruvate carboxylase vs. SwissProt
Match: gi|46397656|sp|Q9KWU4.1|PYC_BACSU (RecName: Full=Pyruvate carboxylase; AltName: Full=Pyruvic carboxylase; Short=PYC)

HSP 1 Score: 539.265 bits (1388), Expect = 2.212e-175
Identity = 252/452 (55.75%), Postives = 337/452 (74.56%), Query Frame = 0

Query:  380 PEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            PE+FSME+WGGATFDV  RFLKEDPW RL  LRK +PN L QMLLRSSNAVGYT YPDN++++F+ ++A++G+D+FRIFDSLNW K M L+I  VR+ T  +AEA ICYTGDI++ N+ K++L YY  +AKELE  GAHIL IKDMAGLL P AA +L++ALKE ++IP+HLHTHDTS      Y KA+EAGVD+IDVA+SSM+GLTSQP+ +   + ++G+ R    ++  +   S YWE +R++Y  FES +K+   ++YE+E+PGGQYSNL+ QA+G+G+ D++  +KE Y+  N++FG +VKVTPSSKVVGDMA++M  N  T++D+  KG+   FPDSV+  FKG+IGQP+GGFP +LQK+ILK Q   T RP E L PV FE    EF+++     +D D ++Y LYPKV+ D+ +  E  GD+ V+ T TFFYGM   EEI +EI
Sbjct:  570 PELFSMEMWGGATFDVAYRFLKEDPWKRLEDLRKEVPNTLFQMLLRSSNAVGYTNYPDNVIKEFVKQSAQSGIDVFRIFDSLNWVKGMTLAIDAVRD-TGKVAEAAICYTGDILDKNRTKYDLAYYTSMAKELEAAGAHILGIKDMAGLLKPQAAYELVSALKETIDIPVHLHTHDTSGNGIYMYAKAVEAGVDIIDVAVSSMAGLTSQPSASGFYHAMEGNDRRPEMNVQGVELLSQYWESVRKYYSEFESGMKSPHTEIYEHEMPGGQYSNLQQQAKGVGLGDRWNEVKEMYRRVNDMFGDIVKVTPSSKVVGDMALYMVQNNLTEKDVYEKGESLDFPDSVVELFKGNIGQPHGGFPEKLQKLILKGQEPITVRPGELLEPVSFEAIKQEFKEQHNLEISDQDAVAYALYPKVFTDYVKTTESYGDISVLDTPTFFYGMTLGEEIEVEI 1020          

HSP 2 Score: 449.899 bits (1156), Expect = 2.490e-141
Identity = 220/416 (52.88%), Postives = 288/416 (69.23%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMP 416
            A TEL IRT+A+Y+ ED  S HRYKADEAY +GE ++P+  YLDIE II +AK+ K DAIHPGYGFLSEN+ FA+RC EEGI FIGP  E +   GDKV A+  A  A +PVI  S      +       +  G+PI++KA+ GGGGRGMR+VR+E E+ +A+  AK+EAK AFG D +++EK IENPKHIEVQ++GD+ GN+VHL+ERDCSVQRR QKV+EVAP+ SL  E +D++ E A+ + K V Y NAGTVEFL+ A    YFIEVNPRVQVEHTITE ITG+DIV+TQIL+A G  L    + I +Q+D+   G+AIQ R+TTEDPQN+F PD G I+AYR+  G G+RLD G+S+ G  I+P++D    K       +  W         + ++     R+R ++  +P
Sbjct:   24 ACTELNIRTVAVYSKEDSGSYHRYKADEAYLVGEGKKPIDAYLDIEGIIDIAKRNKVDAIHPGYGFLSENIHFARRCEEEGIVFIGPKSEHLDMFGDKVKAREQAEKAGIPVIPGSDGPAETLEAVEQFGQANGYPIIIKASLGGGGRGMRIVRSESEVKEAYERAKSEAKAAFGNDEVYVEKLIENPKHIEVQVIGDKQGNVVHLFERDCSVQRRHQKVIEVAPSVSLSPELRDQICEAAVALAKNVNYINAGTVEFLV-ANNEFYFIEVNPRVQVEHTITEMITGVDIVQTQILVAQGHSLHSKKVNIPEQKDIFTIGYAIQSRVTTEDPQNDFMPDTGKIMAYRSGGGFGVRLDTGNSFQGAVITPYYDSLLVK-------LSTWALTFEQAAAKMVRNLQEFRIRGIKTNIP 431          

BLAST of pyruvate carboxylase vs. SwissProt
Match: gi|74957739|sp|O17732.1|PYC1_CAEEL (RecName: Full=Pyruvate carboxylase 1; AltName: Full=Pyruvic carboxylase 1; Short=PCB 1)

HSP 1 Score: 438.343 bits (1126), Expect = 1.293e-136
Identity = 212/474 (44.73%), Postives = 312/474 (65.82%), Query Frame = 0

Query:  361 SYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAV-NIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFE--SELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            +Y    ISPF  +SF      +FS+E WGGATFDV MRFL E PW RL+ LRK +PNI  Q LLR +NA+GY+ YPDN++ KF   A +NGMD+FR+FDSLN+  ++ + +  V  K   + EA I YTGD+ + ++ K++L+YY++LA +L +  AHIL IKDMAG+L P AA+ LI AL++   +IPIH+HTHDTS    A  L+  +AG DV+D A+ SMSG+TSQP+  +I   LQG        +  ++++S YWE  R+ Y PFE  + +K+G A VY++EIPGGQY+NL+ QA  LG+  +F+ +K  Y+ AN + G ++KVTPSSK+VGD+A FM  N  T+E ++ +  D +FP SV++F +G++GQP  GFP  L+  +L+ +     RP E+  PVD +   +E +++ G   ++ D +SY ++P V+ +F  F +  G V  +PT  F  G++  EE+ +EI
Sbjct:  580 TYDMAAISPFVAQSFNG----LFSLENWGGATFDVSMRFLHECPWERLQTLRKLIPNIPFQCLLRGANAMGYSNYPDNVIYKFCELAVKNGMDVFRVFDSLNYLPNLLVGMEAV-GKAGGVVEAAIAYTGDVTDKSRDKYDLKYYLNLADQLVKAQAHILSIKDMAGVLKPEAAKLLIGALRDKFPDIPIHVHTHDTSGAGVAAMLECAKAGADVVDAAVDSMSGMTSQPSMGAIVASLQGTKHDTGLSLDDISKYSAYWESTRQLYAPFECATTMKSGNADVYKHEIPGGQYTNLQFQAFSLGLGPQFDEVKRMYREANLVLGDIIKVTPSSKIVGDLAQFMVQNNLTRETLVDRADDLSFPKSVVDFMQGNVGQPPYGFPEPLRTKVLRGKPKVDGRPGENAKPVDLDAVKVELEEKHGRTLSEEDVMSYSMFPTVFDEFETFRQQYGPVDKLPTRLFLTGLEIAEEVDVEI 1048          

HSP 2 Score: 390.193 bits (1001), Expect = 1.333e-118
Identity = 212/419 (50.60%), Postives = 280/419 (66.83%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDK------SFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRK 413
            A TEL   ++A+Y  +D+ S+HR KADEAY +G+   P+  YL I++II  A K   DAIHPGYGFLSE  +FA  C+  GI FIGPSP+ MA++GDKVAA+  A  A V V+  +   +     A+  AK  G PI++KAA GGGGRG+R V   EE+ +AF  + +EA+ AFG  ++F+EKF+E P+HIEVQLLGD HGN+VHLYERDCSVQRR QKVVE+APAP+L +  ++K+   A+++ + VGY NAGTVEFL+D + N YFIEVN R+QVEHT+TEEITG+D+V+ QI IA G  L    +    QE +   G AIQCR+TTEDP   FQPD G I  +R+  GMGIRLD  S++ G  ISP +D       + A+NHP   +  I     F +  R +K +    L +LR+
Sbjct:   50 ALTELNKTSVAIYAEQDKNSMHRLKADEAYLVGKGLPPVAAYLTIDQIIETALKHNIDAIHPGYGFLSERSDFAAACQNAGIVFIGPSPDVMARMGDKVAARQAAIEAGVQVVPGTPGPITTADEAVEFAKQYGTPIILKAAYGGGGRGIRRVDKLEEVEEAFRRSYSEAQAAFGDGSLFVEKFVERPRHIEVQLLGDHHGNIVHLYERDCSVQRRHQKVVEIAPAPALPEGVREKILADALRLARHVGYQNAGTVEFLVDQKGNYYFIEVNARLQVEHTVTEEITGVDLVQAQIRIAEGKSLDDLKL---SQETIQTTGSAIQCRVTTEDPAKGFQPDSGRIEVFRSGEGMGIRLDSASAFAGSVISPHYDSLMVKVIASARNHPNAAAKMIRALKKFRI--RGVKTNIPFLLNVLRQ 463          

BLAST of pyruvate carboxylase vs. SwissProt
Match: gi|110825736|sp|Q29RK2.2|PYC_BOVIN (RecName: Full=Pyruvate carboxylase, mitochondrial; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor)

HSP 1 Score: 423.705 bits (1088), Expect = 3.597e-131
Identity = 213/468 (45.51%), Postives = 293/468 (62.61%), Query Frame = 0

Query:  367 ISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAV-NIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFE--SELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            ISP+   SF K    +FS+E WGGATFDV MRFL E PW RL+ LR+ +PNI  QMLLR +NAVGYT YPDN+V KF   A ENGMDIFR+FDSLN+  ++ L +         + EA I YTGD+ +P++ K++LQYY+ LA+EL   G HILCIKDMAGLL P A   L+++L++   ++P+H+HTHDTS    A  L    AG DV+DVA  SMSG+TSQP+  ++    +G        + ++ ++S YWE  R  Y  F+  + +K+G + VYENEIPGGQY+NL  QA  +G+  KF+ +K+ Y  AN++ G ++KVTPSSK+VGD+A FM  NG T+ +   + ++ +FP SV+ F +G IG P+GGFP  L+  +LKD      RP   L P+D +    E  +  G   T  D LS  +YP V+  F +F    G +  + T  F  G K  EE  +E+
Sbjct:  590 ISPYVAHSFNK----LFSIENWGGATFDVAMRFLYECPWRRLQELRELVPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDIFRVFDSLNYLPNLLLGMEAA-GSAGGVVEAAISYTGDVSDPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAHAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLTRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQALEKELTERHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVEL 1052          

HSP 2 Score: 401.749 bits (1031), Expect = 5.994e-123
Identity = 205/386 (53.11%), Postives = 272/386 (70.47%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDK------SFAKNHP 380
            A TEL IRT+A+Y+ +D   +HR KADEAY IG    P++ YL I +II +AK+   DA+HPGYGFLSE  +FA+ C++ G++FIGPSPE + ++GDKV A+ IA AA VPV+  +   +  +  A   +   GFPI+ KAA GGGGRGMRVV + EEL + +T A +EA  AFG   +F+EKFIE P+HIEVQ+LGD++GN++HLYERDCS+QRR QKVVE+APA  L  + + +L   ++K+ KQVGY NAGTVEFL+D     YFIEVN R+QVEHT+TEEIT +D+V  QI +A G  L  P + + +QE++ I G AIQCR+TTEDP  +FQPD G I  +R+  GMGIRLD  S++ G  ISP +D       +  K+HP
Sbjct:   55 ACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSL--PDLGL-RQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHP 437          

BLAST of pyruvate carboxylase vs. SwissProt
Match: gi|1709946|sp|P11154.2|PYC1_YEAST (RecName: Full=Pyruvate carboxylase 1; AltName: Full=Pyruvic carboxylase 1; Short=PCB 1)

HSP 1 Score: 422.55 bits (1085), Expect = 9.554e-131
Identity = 208/468 (44.44%), Postives = 306/468 (65.38%), Query Frame = 0

Query:  366 TISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAV-NIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKD-QIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            TI+P    + A      F++E WGGATFDV MRFL EDPW RLR LR  +PNI  QMLLR +N V Y++ PDN ++ F+ +A +NG+DIFR+FD+LN  + +++ +  V+ K   + EA +C++GD++ P K K+NL YY+++A+++ +MG HIL IKDMAG + P AA+ LI +L+    ++PIH+HTHD++    A+      AG DV+DVA++SMSGLTSQP+ N++   L+G+      ++  + E   YW ++R  Y  FE++LK    +VY++EIPGGQ +NL  QA+ LG+ +++   K  Y+ AN L G +VKVTP+SKVVGD+A FM SN  T +D+        FPDSV++FF+G IGQPYGGFP   +  +L++ +   T RP   L P D EK   + Q  FG+   + D  SY +YP+VY+DF +  E  GD+ V+PT +F   ++ +EEI + I
Sbjct:  583 TIAPTTAHALAGR----FALECWGGATFDVAMRFLHEDPWERLRKLRSLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKDNGVDIFRVFDALNDLEQLKVGVDAVK-KAGGVVEATVCFSGDMLQPGK-KYNLDYYLEIAEKIVQMGTHILGIKDMAGTMKPAAAKLLIGSLRAKYPDLPIHVHTHDSAGTAVASMTACALAGADVVDVAINSMSGLTSQPSINALLASLEGNI-DTGINVEHVRELDAYWAEMRLLYSCFEADLKGPDPEVYQHEIPGGQLTNLLFQAQQLGLGEQWAETKRAYREANYLLGDIVKVTPTSKVVGDLAQFMVSNKLTSDDVRRLANSLDFPDSVMDFFEGLIGQPYGGFPEPFRSDVLRNKRRKLTCRPGLELEPFDLEKIREDLQNRFGD-VDECDVASYNMYPRVYEDFQKMRETYGDLSVLPTRSFLSPLETDEEIEVVI 1042          

HSP 2 Score: 384.03 bits (985), Expect = 2.763e-116
Identity = 216/424 (50.94%), Postives = 288/424 (67.92%), Query Frame = 0

Query:    2 ATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEE--PLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMR-FLKEDPWMRLRLLRKAMPNILTQM 422
            A EL ++T+A+Y+ EDR S H+ KADEAY IGE  +  P+  YL I+EII +A+K + D IHPGYGFLSEN EFA +  + GI +IGP  E +  +GDKV+A+ +A  ANVP +  +   +  V  AL      G+P+++KAA GGGGRGMRVVR  ++++ AF  A +EA+ AFG  T F+E+F++ PKHIEVQLL D HGN+VHL+ERDCSVQRR QKVVEVAPA +L +E +D +   A+K+ K+ GY NAGT EFL+D +   YFIE+NPR+QVEHTITEEITGIDIV  QI IA+G  L    +F   Q+ +  +GFAIQCRITTEDP  NFQPD G I  YR+A G G+RLD G++Y G  ISP +D    K            G+T+++  R  ++     R+R ++  +P +LT +
Sbjct:   38 AHELSMQTVAIYSHEDRLSTHKQKADEAYVIGEVGQYTPVGAYLAIDEIISIAQKHQVDFIHPGYGFLSENSEFADKVVKAGITWIGPPAEVIDSVGDKVSARNLAAKANVPTVPGTPGPIETVEEALDFVNEYGYPVIIKAAFGGGGRGMRVVREGDDVADAFQRATSEARTAFGNGTCFVERFLDKPKHIEVQLLADNHGNVVHLFERDCSVQRRHQKVVEVAPAKTLPREVRDAILTDAVKLAKECGYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGASLPQLGLF---QDKITTRGFAIQCRITTEDPAKNFQPDTGRIEVYRSAGGNGVRLDGGNAYAGTIISPHYDSMLVKCS--------CSGSTYEIVRRKMIRALIEFRIRGVKTNIPFLLTLL 450          

BLAST of pyruvate carboxylase vs. SwissProt
Match: gi|585765|sp|P32327.2|PYC2_YEAST (RecName: Full=Pyruvate carboxylase 2; AltName: Full=Pyruvic carboxylase 2; Short=PCB 2)

HSP 1 Score: 422.165 bits (1084), Expect = 1.536e-130
Identity = 209/468 (44.66%), Postives = 304/468 (64.96%), Query Frame = 0

Query:  366 TISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAV-NIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKD-QIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            TI+P    + A      F++E WGGATFDV MRFL EDPW RLR LR  +PNI  QMLLR +N V Y++ PDN ++ F+ +A +NG+DIFR+FD+LN  + +++ +  V+ K   + EA +CY+GD++ P K K+NL YY+++ +++ +MG HIL IKDMAG + P AA+ LI +L+    ++PIH+H+HD++    A+      AG DV+DVA++SMSGLTSQP+ N++   L+G+      ++  + E   YW ++R  Y  FE++LK    +VY++EIPGGQ +NL  QA+ LG+ +++   K  Y+ AN L G +VKVTP+SKVVGD+A FM SN  T +DI        FPDSV++FF+G IGQPYGGFP  L+  +L++ +   T RP   L P D EK   + Q  FG+   + D  SY +YP+VY+DF +  E  GD+ V+PT  F    + +EEI + I
Sbjct:  584 TIAPTTAHALAG----AFALECWGGATFDVAMRFLHEDPWERLRKLRSLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKDNGVDIFRVFDALNDLEQLKVGVNAVK-KAGGVVEATVCYSGDMLQPGK-KYNLDYYLEVVEKIVQMGTHILGIKDMAGTMKPAAAKLLIGSLRTRYPDLPIHVHSHDSAGTAVASMTACALAGADVVDVAINSMSGLTSQPSINALLASLEGNI-DTGINVEHVRELDAYWAEMRLLYSCFEADLKGPDPEVYQHEIPGGQLTNLLFQAQQLGLGEQWAETKRAYREANYLLGDIVKVTPTSKVVGDLAQFMVSNKLTSDDIRRLANSLDFPDSVMDFFEGLIGQPYGGFPEPLRSDVLRNKRRKLTCRPGLELEPFDLEKIREDLQNRFGD-IDECDVASYNMYPRVYEDFQKIRETYGDLSVLPTKNFLAPAEPDEEIEVTI 1043          

HSP 2 Score: 402.134 bits (1032), Expect = 4.605e-123
Identity = 218/425 (51.29%), Postives = 290/425 (68.24%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEE--PLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMR-FLKEDPWMRLRLLRKAMPNILTQM 422
            +A EL +RTIA+Y+ EDR S+HR KADEAY IGE+ +  P+  YL ++EII +AKK K D IHPGYGFLSEN EFA +  + GI +IGP  E +  +GDKV+A+ +A  ANVP +  +   +  V  AL      G+P+++KAA GGGGRGMRVVR  ++++ AF  A +EA+ AFG  T F+E+F++ PKHIEVQLL D HGN+VHL+ERDCSVQRR QKVVEVAPA +L +E +D +   A+K+ K  GY NAGT EFL+D +   YFIE+NPR+QVEHTITEEITGIDIV  QI IA+G  L    +    Q+ +  +GF+IQCRITTEDP  NFQPD G +  YR+A G G+RLD G++Y G TISP +D    K            G+T+++  R  ++     R+R ++  +P +LT +
Sbjct:   38 SAHELSMRTIAIYSHEDRLSMHRLKADEAYVIGEEGQYTPVGAYLAMDEIIEIAKKHKVDFIHPGYGFLSENSEFADKVVKAGITWIGPPAEVIDSVGDKVSARHLAARANVPTVPGTPGPIETVQEALDFVNEYGYPVIIKAAFGGGGRGMRVVREGDDVADAFQRATSEARTAFGNGTCFVERFLDKPKHIEVQLLADNHGNVVHLFERDCSVQRRHQKVVEVAPAKTLPREVRDAILTDAVKLAKVCGYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGIDIVSAQIQIAAGATLTQLGLL---QDKITTRGFSIQCRITTEDPSKNFQPDTGRLEVYRSAGGNGVRLDGGNAYAGATISPHYDSMLVKCS--------CSGSTYEIVRRKMIRALIEFRIRGVKTNIPFLLTLL 451          

BLAST of pyruvate carboxylase vs. SwissProt
Match: gi|1709947|sp|P11498.2|PYC_HUMAN (RecName: Full=Pyruvate carboxylase, mitochondrial; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor)

HSP 1 Score: 421.394 bits (1082), Expect = 2.728e-130
Identity = 210/468 (44.87%), Postives = 293/468 (62.61%), Query Frame = 0

Query:  367 ISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAV-NIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFE--SELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            I+P+   +F+K    +FSME WGGATFDV MRFL E PW RL+ LR+ +PNI  QMLLR +NAVGYT YPDN+V KF   A ENGMD+FR+FDSLN+  +M L +         + EA I YTGD+ +P++ K++LQYY+ LA+EL   G HILCIKDMAGLL P A   L+++L++   ++P+H+HTHDTS    A  L   +AG DV+DVA  SMSG+TSQP+  ++    +G        + ++ ++S YWE  R  Y  F+  + +K+G + VYENEIPGGQY+NL  QA  +G+  KF+ +K+ Y  AN++ G ++KVTPSSK+VGD+A FM  NG ++ +   + ++ +FP SV+ F +G IG P+GGFP   +  +LKD      RP   L P+D +    E     G   T  D LS  +YP V+  F +F    G +  + T  F  G K  EE  +E+
Sbjct:  590 IAPYVAHNFSK----LFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAA-GSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVEL 1052          

HSP 2 Score: 400.979 bits (1029), Expect = 1.137e-122
Identity = 205/386 (53.11%), Postives = 272/386 (70.47%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDK------SFAKNHP 380
            A TEL IRT+A+Y+ +D   +HR KADEAY IG    P++ YL I +II +AK+   DA+HPGYGFLSE  +FA+ C++ G++FIGPSPE + ++GDKV A+ IA AA VPV+  +   +  +  A   +   GFPI+ KAA GGGGRGMRVV + EEL + +T A +EA  AFG   +F+EKFIE P+HIEVQ+LGD++GN++HLYERDCS+QRR QKVVE+APA  L  + + +L   ++K+ KQVGY NAGTVEFL+D     YFIEVN R+QVEHT+TEEIT +D+V  QI +A G  L  P + + +QE++ I G AIQCR+TTEDP  +FQPD G I  +R+  GMGIRLD  S++ G  ISP +D       +  K+HP
Sbjct:   55 ACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSL--PDLGL-RQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHP 437          

BLAST of pyruvate carboxylase vs. SwissProt
Match: gi|51701714|sp|Q9UUE1.1|PYC_SCHPO (RecName: Full=Pyruvate carboxylase; AltName: Full=Pyruvic carboxylase; Short=PCB)

HSP 1 Score: 419.853 bits (1078), Expect = 1.189e-129
Identity = 209/469 (44.56%), Postives = 303/469 (64.61%), Query Frame = 0

Query:  365 VTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEA-VNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQI-AFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            V I+P+   + A      +S+E+WGGATFDV MRFL E PW RLR LRK +PNI  QMLLR +N + Y++ PDN++  F  +A +NG+DIFR+FD+LN   ++ L I   + +   + EA +CY+GD++NP K+K+NL YYV+L  ++ EMG HIL IKDMAG++ P AA  LI+A++E    +PIH+HTHD++    A+   A+EAG DV+DVA  SMSGLTSQP+F ++   + G  +    D  +L E  +YW  +R  Y PFESE+K   + VY +EIPGGQ +NL+ QA  LG+  ++   K+ Y  AN+L G ++KVTP+SKVVGD+A FM  N  + ED+  +     FP SV++FF+G +GQPYGGFP  L+  +LK +    TDRP + L   DF+       ++FG   +D D  +Y  +P V++++ +F +  GD+  +PT  F    + NEE+ +EI
Sbjct:  595 VNIAPYTSHALA----SAYSLEMWGGATFDVSMRFLHECPWDRLRRLRKLVPNIPFQMLLRGANGLCYSSLPDNVIYFFCEQAKKNGIDIFRVFDALNDVNNLSLGIDAAK-RAGGVVEATMCYSGDMLNP-KKKYNLDYYVNLVDKMVEMGIHILGIKDMAGVMKPKAARLLISAIREKHPELPIHVHTHDSAGTAVASMAAALEAGADVVDVATDSMSGLTSQPSFGAVLASVDGTDKQLEFDNNQLREIDSYWAQMRLLYSPFESEIKGTDSDVYNHEIPGGQLTNLKFQATSLGLGTQWAETKKAYIEANKLLGDIIKVTPTSKVVGDLAQFMVQNKLSAEDVENRATTLDFPASVLDFFQGLMGQPYGGFPEPLRTNVLKGRRQPLTDRPGKFLPAADFDAIRKLLSEKFGV-SSDCDIAAYTQFPGVFEEYRQFVDRYGDLTTVPTKFFLSRPEMNEEMHVEI 1056          

HSP 2 Score: 382.104 bits (980), Expect = 1.190e-115
Identity = 211/454 (46.48%), Postives = 300/454 (66.08%), Query Frame = 0

Query:    2 ATELKIRTIALYTFEDRYSLHRYKADEAYQIGE--DEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMR-FLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVG--YTAYPDNLVEKFIIKAAEN 450
            A EL + T+A+Y++EDR S+HR KADE+Y IG+     P+  YL I+EI+ +AK+  A+ +HPGYGFLSEN EFA++  E G++F+GPSPE +  LGDK  A+ IA    VPV+  +   +     A +  K  G P+++KAA GGGGRGMRVVR+ + L ++F  A++EA  +FG  T+F+E+F++ PKHIE+QL+ D+ GN++HL+ERDCSVQRR QKVVE+APA  L  + +  LY+ AIKI K+V Y NAGT EFLLD +   YFIE+NPR+QVEHTITEEITG+DIV  Q+ +A+G  L  P I +T Q+ ++ +GFAIQCR+TTEDP N F PD G I  YR+A G G+RLD  + + G  I+P +D    K             AT++   R  ++     R+R ++  +P +L ++L+  +   G  +T + D+  E F +  + N
Sbjct:   52 AHELSMHTVAIYSYEDRLSMHRQKADESYPIGKVGQYSPVGAYLAIDEIVSIAKRTGANLVHPGYGFLSENAEFARKVNEAGMQFVGPSPEVIDSLGDKTKARAIAIRCGVPVVPGTPGPVEHYEEAEAFVKEYGLPVIIKAAMGGGGRGMRVVRSADTLKESFERARSEALASFGDGTVFIERFLDKPKHIEIQLMADKAGNVIHLHERDCSVQRRHQKVVEIAPAKDLDPKIRQALYDDAIKIAKEVKYCNAGTAEFLLDQKGRHYFIEINPRIQVEHTITEEITGVDIVSAQLHVAAGFTL--PEIGLT-QDKISTRGFAIQCRVTTEDPNNGFAPDIGKIEVYRSAGGNGVRLDGANGFAGSVITPHYDSMLVKC--------TCHDATYEYTRRKMIRSLIEFRVRGVKTNIPFVL-RLLMHDTFIQGNCWTTFIDDTPELFQLYRSRN 493          

BLAST of pyruvate carboxylase vs. SwissProt
Match: gi|51701711|sp|Q8X1T3.1|PYC_PICAN (RecName: Full=Pyruvate carboxylase; AltName: Full=Pyruvic carboxylase; Short=PCB)

HSP 1 Score: 419.083 bits (1076), Expect = 1.994e-129
Identity = 212/453 (46.80%), Postives = 298/453 (65.78%), Query Frame = 0

Query:  383 FSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITAL-KEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLN--EFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILK-DQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            F++E WGGATFDV MRFL EDPW RLR LRKA+PNI  QMLLR +N V Y++ PDN ++ F+ +A + G+DIFR+FD+LN  + +++ +  V+ K   + EA +CY+GD++ P K K+NL+YY++   ++ EMG H+L IKDMAG L P AA+ LI+++ K+  ++PIH+HTHD++     TY+    AG DV+D A++SMSGLTSQP+ ++    L       NT IT+ N  E   YW ++R  Y  FE++LK    +VY +EIPGGQ +NL  QA+ +G+ +K+   K+ Y+ AN L G +VKVTP+SKVVGD+A FM SN  + +D+     +  FPDSV++FF+G +G PYGGFP  L+  IL   +   T RP   L P D +K   E Q  FGN  T+ D  SY +YPKV++ F +  E  GD+ V+PT  F    K NEEI +EI
Sbjct:  600 FALECWGGATFDVAMRFLHEDPWDRLRKLRKAVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKDAGVDIFRVFDALNDLEQLKVGVDAVK-KAGGVVEATVCYSGDMLKPGK-KYNLKYYLETVDKIMEMGTHLLGIKDMAGTLKPAAAKLLISSIRKKYPSVPIHVHTHDSAGTGVITYVACALAGADVVDCAVNSMSGLTSQPSMSAFIAALDNEI---NTGITEQNAREIDAYWSEMRLLYSCFEADLKGPDPEVYNHEIPGGQLTNLLFQAQQVGLGEKWLETKKAYEEANMLLGDIVKVTPTSKVVGDLAQFMVSNKLSPKDVERLASELDFPDSVLDFFEGLMGTPYGGFPEPLRTNILAGKRRKLTRRPGLELEPFDLKKIKEELQSRFGNSITECDVASYNMYPKVFESFKKIQEKYGDLSVLPTRFFLAPPKLNEEISVEI 1047          

HSP 2 Score: 361.688 bits (927), Expect = 3.667e-108
Identity = 202/425 (47.53%), Postives = 279/425 (65.65%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEE--PLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMR-FLKEDPWMRLRLLRKAMPNILTQM 422
            +A EL ++T+A+Y+ EDR S+HR KADEAY IG   +  P++ YL I+EII +A +     IHPGYGFLSEN EFA++  + G+ +IGP    +  +GDKV+A+ +A   NVPV+  +   ++ V  A       G+P+++KAA GGGGRGMRVVR  E ++ AF  A +EAK AFG  T F+E+F++ PKHIEVQLL D +GN++HL+ERDCSVQRR QKVVE+APA +L  E +D +   A+K+ K   Y NAGT EFL+D +   YFIE+NPR+QVEHTITEE+TG+DIV  QI IA+G  L    +    Q+ +  +GFAIQCRITTEDP  NFQPD G I  YR++ G G+RLD G+ + G  ISP +D    K            G+ +++  R  ++     R+R ++  +P +L  +
Sbjct:   41 SAHELSMQTVAIYSHEDRLSMHRLKADEAYVIGARGQYSPVQAYLQIDEIINIALEHNVSMIHPGYGFLSENSEFARKVEDSGMIWIGPPHNVIDAVGDKVSARNLAGKCNVPVVPGTDGPIDSVEQAQEFVDKYGYPVIIKAAFGGGGRGMRVVREGESIADAFQRATSEAKTAFGNGTCFIERFLDKPKHIEVQLLADNYGNVIHLFERDCSVQRRHQKVVEIAPAKTLPVEVRDAILTDAVKLAKAANYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEVTGVDIVAAQIQIAAGASLQQLGLL---QDKITTRGFAIQCRITTEDPAKNFQPDTGKIEVYRSSGGNGVRLDGGNGFAGAIISPHYDSMLVKCSTS--------GSNYEIARRKMIRALVEFRIRGVKTNIPFLLALL 454          

BLAST of pyruvate carboxylase vs. SwissProt
Match: gi|464506|sp|Q05920.1|PYC_MOUSE (RecName: Full=Pyruvate carboxylase, mitochondrial; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor)

HSP 1 Score: 416.001 bits (1068), Expect = 2.884e-128
Identity = 207/468 (44.23%), Postives = 294/468 (62.82%), Query Frame = 0

Query:  367 ISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAV-NIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFE--SELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            I+P+   +F K    +FSME WGGATFDV MRFL E PW RL+ LR+ +PNI  QMLLR +NAVGYT YPDN+V KF   A ENGMD+FR+FDSLN+  +M L +         + EA I YTGD+ +P++ K++L+YY+ LA+EL   G HILCIKDMAGLL P A   L+++L++   ++P+H+HTHDTS    A  L   +AG DV+DVA+ SMSG+TSQP+  ++    +G        + ++ ++S YWE  R  Y  F+  + +K+G + VYENEIPGGQY+NL  QA  +G+  KF+ +K+ Y  AN++ G ++KVTPSSK+VGD+A FM  NG ++ +   + ++ +FP SV+ F +G IG P+GGFP   +  +LKD      RP   L P++ ++   +     G   T  D LS  +YP V+  F +F    G +  + T  F  G K  EE  +E+
Sbjct:  590 IAPYVAHNFNK----LFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAA-GSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVEL 1052          

HSP 2 Score: 400.208 bits (1027), Expect = 2.416e-122
Identity = 204/386 (52.85%), Postives = 273/386 (70.73%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDK------SFAKNHP 380
            A TEL IRT+A+Y+ +D   +HR KADEAY IG    P++ YL I +II +AK+   DA+HPGYGFLSE  +FA+ C++ G++FIGPSPE + ++GDKV A+ IA AA VPV+  +   ++ +  A   +   GFPI+ KAA GGGGRGMRVV + EEL + +T A +EA  AFG   +F+EKFIE P+HIEVQ+LGD++GN++HLYERDCS+QRR QKVVE+APA  L  + + +L   ++K+ KQVGY NAGTVEFL+D     YFIEVN R+QVEHT+TEEIT +D+V  QI ++ G  L  P + + +QE++ I G AIQCR+TTEDP  +FQPD G I  +R+  GMGIRLD  S++ G  ISP +D       +  K+HP
Sbjct:   55 ACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDSPISSLHEAHEFSNTFGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSL--PDLGL-RQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHP 437          

BLAST of pyruvate carboxylase vs. SwissProt
Match: gi|146345499|sp|P52873.2|PYC_RAT (RecName: Full=Pyruvate carboxylase, mitochondrial; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor)

HSP 1 Score: 412.92 bits (1060), Expect = 3.695e-127
Identity = 207/468 (44.23%), Postives = 293/468 (62.61%), Query Frame = 0

Query:  367 ISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAV-NIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFE--SELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            I+P+     A N   +FS+E WGGATFDV MRFL E PW RL+ LR+ +PNI  QMLLR +NAVGYT YPDN+V KF   A ENGMD+FRIFDSLN+  +M L +         + EA I YTGD+ +P++ K++L+YY+ LA+EL   G HILCIKDMAGLL P A   L+++L++   ++P+H+HTHDTS    A  L   +AG DV+DVA+ SMSG+TSQP+  ++    +G        + ++ ++S YWE  R  Y  F+  + +K+G + VYENEIPGGQY+NL  QA  +G+  KF+ +K+ Y  AN++ G ++KVTPSSK+VGD+A FM  NG ++ +   + ++ +FP SV+ F +G IG P+GGFP   +  +LKD      RP   L P++ ++   +     G   T  D LS  +YP V+  F +F    G +  + T  F  G K  EE  +E+
Sbjct:  590 IAPYV----AHNFNNLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRIFDSLNYLPNMLLGMEAA-GSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGSGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVEL 1052          

HSP 2 Score: 401.749 bits (1031), Expect = 6.247e-123
Identity = 205/386 (53.11%), Postives = 273/386 (70.73%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDK------SFAKNHP 380
            A TEL IRT+A+Y+ +D   +HR KADEAY IG    P++ YL I +II +AK+   DA+HPGYGFLSE  +FA+ C++ G++FIGPSPE + ++GDKV A+ IA AA VPV+  +   +N +  A   +   GFPI+ KAA GGGGRGMRVV + EEL + +T A +EA  AFG   +F+EKFIE P+HIEVQ+LGD++GN++HLYERDCS+QRR QKVVE+APA  L  + + +L   ++K+ KQVGY NAGTVEFL+D     YFIEVN R+QVEHT+TEEIT +D+V  QI ++ G  L  P + + +QE++ I G AIQCR+TTEDP  +FQPD G I  +R+  GMGIRLD  S++ G  ISP +D       +  K+HP
Sbjct:   55 ACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTNSPINSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSL--PDLGL-RQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHP 437          

BLAST of pyruvate carboxylase vs. nr
Match: gi|503969247|ref|WP_014203241.1| (pyruvate carboxylase [Owenweeksia hongkongensis] >gi|359349473|gb|AEV33892.1| pyruvate carboxylase [Owenweeksia hongkongensis DSM 17368])

HSP 1 Score: 752.666 bits (1942), Expect = 0.000e+0
Identity = 342/460 (74.35%), Postives = 403/460 (87.61%), Query Frame = 0

Query:  373 KSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEIS 832
            +SFAK+HPE+FSMEIWGGATFDVCMRFLKEDPW+RL+LLR+AMPN+LTQMLLR+SNAVGY AYPDNLVEKFI K+ E G+DIFRIFDSLNWT+S+++S + VRE+TN +AE C+CYTGDI +    K+ LQYY+DLA+++E+MGAHIL IKDMAGLL P+AA  LIT L++AV+IPIHLHTHDT+ IQ+ATYLKAIEA VD+IDVALSSMSGLTSQPNFNS   M+QGH R    D+ KLNEF+NYWED+RE+YYPFESELKAGTAQVYE+EIPGGQYSNLRPQARGLGIEDKFETIK NY+ AN + G +VKVTPSSKVVGDMAMFMT+NGYT EDI+ KG D +FPDSVI+FFKG++GQP+GGFP  L+K ILKD+  F+DRPN HL PVDF+KEF EFQK+FG+  + LDFLSYKLYPKVYKD+H+FN  NG+VW IPT  FFYGM  NEEIL+E++
Sbjct:  562 ESFAKSHPEIFSMEIWGGATFDVCMRFLKEDPWLRLQLLREAMPNVLTQMLLRASNAVGYKAYPDNLVEKFIEKSWETGVDIFRIFDSLNWTESLKVSTKAVRERTNGLAEVCLCYTGDISSDKHNKYGLQYYLDLARQIEDMGAHILAIKDMAGLLKPYAATTLITELRKAVDIPIHLHTHDTAGIQTATYLKAIEADVDIIDVALSSMSGLTSQPNFNSTVAMMQGHERETRLDLPKLNEFANYWEDVREWYYPFESELKAGTAQVYEHEIPGGQYSNLRPQARGLGIEDKFETIKSNYQKANLMMGDLVKVTPSSKVVGDMAMFMTTNGYTPEDILEKGNDISFPDSVISFFKGELGQPHGGFPEALRKTILKDEPGFSDRPNAHLEPVDFDKEFAEFQKKFGDQTSFLDFLSYKLYPKVYKDYHDFNVENGEVWRIPTLAFFYGMNLNEEILVELA 1021          

HSP 2 Score: 562.377 bits (1448), Expect = 0.000e+0
Identity = 285/377 (75.60%), Postives = 328/377 (87.00%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAK 377
            AA+EL IRTIA+YT+EDRYSLHRYKADEAYQIG D+E LKPYLDIEEII LAKK+ ADAIHPGYGFLSENV+FAKRCREEGI F+GP+PE+MA+LGDK+ AK++ARAANVPVIEDS+ D+     AL+ AK IGFP+M+KAAAGGGGRGMRVVR E  L ++F EA NEA KAFG  T+F+EK+++NPKHIEVQLLGD  GNLVHLYERDCSVQRRFQKVVEVAP+  L Q+T+DKLY YA++IG++V Y NAGTVEFL+D +ENI+FIEVNPR+QVEHTITEEITG+DIVR+QI IA G  L   SIFI  Q+DV I GFAIQCRITTE+P++NF+PDYGTIIAYRNAAGMGIRLDEGSSYPGV ISPFFD    K
Sbjct:   22 AASELNIRTIAIYTYEDRYSLHRYKADEAYQIGPDDEQLKPYLDIEEIITLAKKKNADAIHPGYGFLSENVDFAKRCREEGIIFVGPTPEAMARLGDKIQAKVVARAANVPVIEDSK-DLKTEDDALAAAKKIGFPVMLKAAAGGGGRGMRVVRDEALLKQSFKEASNEALKAFGNGTVFMEKYVDNPKHIEVQLLGDNFGNLVHLYERDCSVQRRFQKVVEVAPSTGLSQDTRDKLYNYALQIGREVKYSNAGTVEFLVDEQENIFFIEVNPRIQVEHTITEEITGVDIVRSQIHIADGKRLDDKSIFIKSQDDVQINGFAIQCRITTENPKDNFKPDYGTIIAYRNAAGMGIRLDEGSSYPGVKISPFFDSMLVK 397          

BLAST of pyruvate carboxylase vs. nr
Match: gi|496346223|ref|WP_009055401.1| (pyruvate carboxylase [Nitritalea halalkaliphila] >gi|389664524|gb|EIM76016.1| pyruvate carboxylase [Nitritalea halalkaliphila LW7])

HSP 1 Score: 743.421 bits (1918), Expect = 0.000e+0
Identity = 336/460 (73.04%), Postives = 397/460 (86.30%), Query Frame = 0

Query:  373 KSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEIS 832
            +S+A++HPE+FS+E+WGGATFDVCMRFLKEDPW RLR LR AMPNILTQML RSSNAVGYT+YPDNLVE+FI +AA NGMD+FRIFDSLNWT++ME+SIR VRE+T S+AEACICYTGDI NP K KF L YY+++AK+LE+MGAHILCIKDMAGLL P+AA +LI +LK+ V +PIHLHTHDTSSIQSATYLKAIEAGVD++DVA+SSMSGLTSQPNFNS+  M+QGH R    D+  LN F+NYWED+RE YYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIE KFETIKENY++AN L G +VKVTPSSKVVGD+A+FMTSNGYT +D++++G+  +FPDSVINF KGD+GQPYGGFP + QKI+LK+   +T RPNE L P+DFE+E   FQ++FG  K   DFLSY LYPKVY++FH F E  G+V  +P+  FFYGMKQNEEI+ EIS
Sbjct:  565 ESYARHHPELFSVELWGGATFDVCMRFLKEDPWERLRKLRAAMPNILTQMLFRSSNAVGYTSYPDNLVERFIEEAAGNGMDVFRIFDSLNWTENMEISIRAVRERTQSLAEACICYTGDISNPEKTKFTLDYYIEVAKKLEDMGAHILCIKDMAGLLKPYAAAELIDSLKQHVQLPIHLHTHDTSSIQSATYLKAIEAGVDIVDVAISSMSGLTSQPNFNSLVAMMQGHEREQKIDLGSLNRFANYWEDVRELYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEHKFETIKENYRVANMLLGDIVKVTPSSKVVGDLALFMTSNGYTAQDLLSRGESLSFPDSVINFLKGDLGQPYGGFPRDFQKIVLKEVQPYTHRPNELLEPIDFERELPAFQEKFGKEKGICDFLSYSLYPKVYQEFHSFCENYGNVLPLPSKAFFYGMKQNEEIVFEIS 1024          

HSP 2 Score: 586.645 bits (1511), Expect = 0.000e+0
Identity = 292/416 (70.19%), Postives = 341/416 (81.97%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMP 416
            A +ELKIRT+A+YT EDRYSLHRYKADEAYQIG++ +PLKPYLD+E II LAK +K DAIHPGYGFLSENV FA+RCREEGI F+GPSPE+M +LGDKVAAK IAR + VPVIEDS +D+ +   AL+EA+ IGFP+M+KAA+GGGGRGMRVVR   EL K+F EA NEA KAFG  T+F+EK+IENPKHIEVQ+LGD+HGN+VHLYERDCSVQRRFQKVVEVAP+P+L Q TKDKLY YA+KI   VGYYNAGTVEFL+D EEN+YFIEVNPR+QVEHTITEEITGIDIVR+Q+LIA G PL+ P I+++ QEDV + GFAIQCR+TTEDP NNFQPD+GTIIAYRNAAGMGIRLDEGSSY GVTISPFFD    K       +  WG        R  +     R+R ++  +P
Sbjct:   24 AGSELKIRTVAIYTHEDRYSLHRYKADEAYQIGKEHDPLKPYLDVEAIIQLAKDKKIDAIHPGYGFLSENVRFARRCREEGILFVGPSPEAMEKLGDKVAAKKIARESEVPVIEDSLVDLQDAELALAEAERIGFPVMLKAASGGGGRGMRVVREASELKKSFKEASNEALKAFGEGTVFIEKYIENPKHIEVQVLGDQHGNIVHLYERDCSVQRRFQKVVEVAPSPTLSQATKDKLYAYALKIAHHVGYYNAGTVEFLVDKEENVYFIEVNPRIQVEHTITEEITGIDIVRSQLLIAQGHPLSFPGIYLSSQEDVKVNGFAIQCRVTTEDPTNNFQPDFGTIIAYRNAAGMGIRLDEGSSYSGVTISPFFDSMLVK-------VSSWGRTLRGAAERLDRALSEFRIRGVKTNIP 432          

BLAST of pyruvate carboxylase vs. nr
Match: gi|918743994|ref|WP_052597410.1| (pyruvate carboxylase [Aureispira sp. CCB-QB1])

HSP 1 Score: 737.643 bits (1903), Expect = 0.000e+0
Identity = 336/459 (73.20%), Postives = 400/459 (87.15%), Query Frame = 0

Query:  373 KSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            +SFAKN+P +FSME+WGGATFDV +RFLKE PW RL+LLRKAMPN L QMLLR SNAVGYTAYPDNLVEKFI KAAE G+DIFR+FDSLNW ++M++SIRT+RE+TNS+AEACICYTGDI NPNK K+ LQYY+DLA++LE+ GAH+LCIKDMAGLL P+AAE+LIT LKEAV++PIHLHTHDTSSIQSATYLKAIEAGVDV+DVALS++SGLTSQPNFNSIA+MLQ H R ++ D+  LN FSNYWE +REFYYPFESELKAGTA+VY++EIPGGQYSNLRPQARGLG+ED+FETIK NY + N LFG +VKVTPSSKVVGDMAMFMT+N YT+ DI+TKG    FPDSV   F+G++GQP+GGFP+ELQKI+LK++  F +RPN HLAP+DFEKEF  FQ+EF     +LDF+SYKLYPKV++DF+E +E    V  +PT  FFYG+KQNEE ++EI
Sbjct:  569 ESFAKNNPNIFSMEVWGGATFDVALRFLKECPWERLKLLRKAMPNTLLQMLLRGSNAVGYTAYPDNLVEKFIEKAAETGIDIFRVFDSLNWVEAMKVSIRTIRERTNSLAEACICYTGDINNPNKTKYTLQYYLDLARQLEDEGAHLLCIKDMAGLLQPYAAEQLITGLKEAVDLPIHLHTHDTSSIQSATYLKAIEAGVDVVDVALSAVSGLTSQPNFNSIASMLQFHERASDLDVQSLNSFSNYWEAVREFYYPFESELKAGTAEVYQHEIPGGQYSNLRPQARGLGLEDQFETIKHNYGVVNRLFGDLVKVTPSSKVVGDMAMFMTANNYTENDILTKGTTIDFPDSVKALFRGNLGQPHGGFPSELQKIVLKEEQPFVERPNAHLAPIDFEKEFKAFQEEFSTFCDELDFISYKLYPKVFRDFYEHHEKFNAVQHLPTLPFFYGLKQNEETIVEI 1027          

HSP 2 Score: 542.347 bits (1396), Expect = 5.877e-174
Identity = 275/433 (63.51%), Postives = 334/433 (77.14%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMP---NILTQMLLRSSNAV 430
            AA+EL++ T+A+YT EDRYSLHRYKADEAYQ+G+D+EPLKPYLDIEEII +AK+   +AIHPGYGFLSENV FA+RC+EEGI F+GPSP +M QLGDKV AK IAR   VP+IEDSQ+ +  V  AL EA  IG+PIMVKAA+GGGGRGMRVVR E +L KA++EAK EAK AFG DT+FLEK+I+ PKHIEVQ+L D +GN+VHL+ERDCS+QRRFQKVVEVAP+ +L Q TKD LYEY+++I K V Y NAGTVEFL+D +ENIYFIEVNPR+QVEHTITE+ITGIDIVR+QILIA G PL+HP I+I  Q+ V+  G+AIQCRITTEDP   F+PDYG +IAYR+AAG GIRLD GS+Y G  ISPFFD    K       +  WG        R  +     R+R ++  +P   N+LT    ++  A 
Sbjct:   27 AASELELTTVAIYTHEDRYSLHRYKADEAYQVGKDDEPLKPYLDIEEIIRIAKENAVEAIHPGYGFLSENVRFAQRCQEEGIVFVGPSPSAMQQLGDKVDAKEIARKLEVPLIEDSQIPLTSVEIALEEAARIGYPIMVKAASGGGGRGMRVVRQESDLPKAYSEAKGEAKTAFGDDTVFLEKYIDAPKHIEVQILADNYGNMVHLFERDCSLQRRFQKVVEVAPSATLGQNTKDALYEYSLRIAKHVNYSNAGTVEFLVDKDENIYFIEVNPRIQVEHTITEQITGIDIVRSQILIAMGYPLSHPQIYILSQQSVSHMGYAIQCRITTEDPSEGFKPDYGQLIAYRSAAGFGIRLDAGSAYTGAKISPFFDSMLVK-------VTAWGRTLKGASGRLHRALREYRIRGVKTNIPFLLNLLTDETFQAGKAT 452          

BLAST of pyruvate carboxylase vs. nr
Match: gi|1057003001|ref|WP_068414518.1| (pyruvate carboxylase [Roseivirga echinicomitans] >gi|1005895250|gb|KYG78801.1| pyruvate carboxylase [Roseivirga echinicomitans])

HSP 1 Score: 702.205 bits (1811), Expect = 0.000e+0
Identity = 314/457 (68.71%), Postives = 390/457 (85.34%), Query Frame = 0

Query:  375 FAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            FAKNHPE+FS+E+WGGATFDV MRFL E+PW RL+L+R+A PNILTQMLLR SN VGY AYPDNL+E+F+ K AENGMD+FRIFDSLNW ++M++SIRTVRE+TNS+AEACICYTGD+++PN  K+NLQYY+D+AK LE+ GAHILCIKDMAGLL P AA KLITALKE+V++PIHLHTHDTSS+Q+ TY +AIEAGVD++DVA++SMSGLTSQPNFNS+   L+GH R    ++ KLNE+SNYWED+RE+YYPFES LKAGTA+VY++EIPGGQYSNLRPQA  LG+E KFE +K+NY + NELFG +VKVTPSSKVVGDMA++MTSN  T ED+MT+G+  +FP+SV++ FKGD+GQ  GGFP  L KIILK + A+TDRPN HL PVDFEK+F +FQ +F  +  +LDFLSYKLYPKV++DF+E     G+V  +PT  FFYG+K+NEE+ ++I
Sbjct:  569 FAKNHPEIFSLEMWGGATFDVSMRFLNENPWDRLKLIREAAPNILTQMLLRGSNGVGYAAYPDNLIERFVEKTAENGMDVFRIFDSLNWIENMKVSIRTVRERTNSVAEACICYTGDVLDPN-SKYNLQYYLDMAKALEDEGAHILCIKDMAGLLKPMAATKLITALKESVDMPIHLHTHDTSSMQATTYFQAIEAGVDIVDVAVASMSGLTSQPNFNSLVACLEGHERDPKINLIKLNEYSNYWEDVREYYYPFESGLKAGTAEVYDHEIPGGQYSNLRPQAAALGLEHKFEEVKKNYAVVNELFGDIVKVTPSSKVVGDMAIYMTSNNLTVEDVMTRGESLSFPESVVSLFKGDLGQTLGGFPQALSKIILKGKTAYTDRPNAHLKPVDFEKDFKQFQTDFDQYCDELDFLSYKLYPKVFRDFYEHWLTYGEVRHLPTKAFFYGLKENEEVFVQI 1024          

HSP 2 Score: 538.495 bits (1386), Expect = 1.675e-172
Identity = 276/377 (73.21%), Postives = 316/377 (83.82%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAK 377
            AA+ELKIRT+A+YT+EDRYSLHRYKADEAYQIG++EE LKPYLDI+EII LAK E  DAIHPGYGFLSENV F + C+E GI FIGP PE M +LGDKV AKIIAR A VP+IEDS   ++     L EA+ +GFP+MVKAAAGGGGRGMRVVR E EL K+F EAK+EA KAFG DT+F+EKFI+NPKHIEVQ++GD +G+LVHLYERDCSVQRR+QKVVEVAP+ +L Q T+DKLYEYA+KI K+V Y N GTVEFL+DA+ENI+FIEVNPRVQVEHTITEEITGIDIVR+QI IA G PL+ P IFI  Q DV   GFAIQCRITTEDP+N F+PDYGTIIAYR+A G GIRLD G+ Y GVTISPFFD    K
Sbjct:   26 AASELKIRTVAMYTYEDRYSLHRYKADEAYQIGDNEEALKPYLDIQEIITLAKNEGVDAIHPGYGFLSENVHFVRACKEAGIIFIGPEPEVMERLGDKVQAKIIARKAQVPLIEDSHELLDSYEVVLKEARRVGFPVMVKAAAGGGGRGMRVVREEVELEKSFNEAKSEASKAFGDDTMFMEKFIDNPKHIEVQIMGDNYGHLVHLYERDCSVQRRYQKVVEVAPSINLSQATRDKLYEYALKITKEVNYNNVGTVEFLVDADENIFFIEVNPRVQVEHTITEEITGIDIVRSQIEIAQGYPLSDPRIFIKDQSDVQCNGFAIQCRITTEDPENGFKPDYGTIIAYRSAGGFGIRLDAGNCYAGVTISPFFDSMLVK 402          

BLAST of pyruvate carboxylase vs. nr
Match: gi|780071412|ref|WP_045459757.1| (pyruvate carboxylase [Sporocytophaga myxococcoides] >gi|695128144|dbj|GAL83965.1| pyruvate carboxylase [Sporocytophaga myxococcoides])

HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0
Identity = 312/460 (67.83%), Postives = 399/460 (86.74%), Query Frame = 0

Query:  373 KSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEIS 832
            +SFAKNHP+VFSME+WGGATFDV MRFL EDPW RL+L+R+A+PNIL QML+R SNAVGY AYPDNL+EKFI K  ENG+DIFRIFDSLNW ++M++SI+ V+E+T  IAEA +CY+GDI++P+ +K+ LQYY+D+A++LE++GAHILCIKDMAGLL P++AE LI  LK+AV+IPIHLHTHDTS+IQ+ATYLKA+EAGVD+IDVA++SMSGLTSQPNFNSI +ML+GH R    +++ LN++SNYWED+REFYYPFES+LKAGTA+VYENEIPGGQYSNLRPQA  LG+ +KFE +K+NY + N++FG ++KVTPSSKVVGDMA+FMTSN  T++DIM KG+  +FP+SV++ FKG+IGQ YGGFP +LQ IILK +  FT+RPN+HLAPVDF+KEF  FQ++FG+  T LDFLSYK+YPKV++ + +  E+ GDV  IPT  FFYG+K N+EIL+++S
Sbjct:  566 ESFAKNHPQVFSMEVWGGATFDVAMRFLHEDPWQRLQLIREAVPNILLQMLIRGSNAVGYKAYPDNLIEKFIEKTWENGVDIFRIFDSLNWVEAMKVSIKAVKERTGGIAEAAVCYSGDILDPDNKKYTLQYYLDMARQLEDLGAHILCIKDMAGLLKPYSAELLIKELKKAVSIPIHLHTHDTSAIQAATYLKAVEAGVDIIDVAIASMSGLTSQPNFNSIVSMLKGHERELKFNMSALNKYSNYWEDVREFYYPFESDLKAGTAEVYENEIPGGQYSNLRPQAEALGLGEKFEKVKQNYALVNKMFGDIIKVTPSSKVVGDMALFMTSNNLTEKDIMEKGQYLSFPESVVSLFKGEIGQAYGGFPEKLQSIILKGEKPFTERPNDHLAPVDFDKEFDAFQQQFGSSSTFLDFLSYKMYPKVFEGYAKHRELYGDVSNIPTLAFFYGLKNNQEILVKLS 1025          

HSP 2 Score: 543.117 bits (1398), Expect = 3.037e-174
Identity = 271/377 (71.88%), Postives = 314/377 (83.29%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAK 377
            AA+ELKI+T+A+YT+EDRYSLHRYKADEAYQIG D EPLKPYLDIE+II LAK+   DAIHPGYGFLSENV  A+RC+EEGI FIGP PE M  LGDKV+AK IA +A VP+IEDS+ ++  +  A  EA  IG+P+M+KAAAGGGGRGMRV+R E +L +A+ EA+ EA KAFG DT+FLEKFI+ PKHIEVQL+GD HGN+VHLYERDCSVQRRFQKVVEVAP P LK+ETK ++Y YA+ I + V Y N GTVEFL+D +E +YFIEVNPR+QVEHTITEEITGIDIVR+QILIA G  L+ P IFI +Q+DV   GFAIQCR+TTEDP N F+PDYGTIIAYRNAAG GIRLDEGSSYPGV ISPFFD    K
Sbjct:   25 AASELKIKTVAIYTYEDRYSLHRYKADEAYQIGRDNEPLKPYLDIEQIIQLAKRHHVDAIHPGYGFLSENVNLARRCKEEGIIFIGPEPEVMEALGDKVSAKEIAISAGVPIIEDSKENLVSLEIAKKEALRIGYPLMMKAAAGGGGRGMRVIRDESQLERAYNEARAEALKAFGDDTVFLEKFIDRPKHIEVQLMGDNHGNIVHLYERDCSVQRRFQKVVEVAPCPVLKEETKQQIYNYALTIARHVKYNNVGTVEFLVDQDERVYFIEVNPRIQVEHTITEEITGIDIVRSQILIAKGHKLSDPCIFIYRQDDVKCNGFAIQCRVTTEDPANGFKPDYGTIIAYRNAAGFGIRLDEGSSYPGVKISPFFDSLLVK 401          

BLAST of pyruvate carboxylase vs. nr
Match: gi|497268703|ref|WP_009582920.1| (pyruvate carboxylase [Fulvivirga imtechensis] >gi|441435353|gb|ELR68749.1| Pyruvate carboxyl transferase [Fulvivirga imtechensis AK7])

HSP 1 Score: 696.041 bits (1795), Expect = 0.000e+0
Identity = 316/459 (68.85%), Postives = 387/459 (84.31%), Query Frame = 0

Query:  373 KSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            + +AKNHP +FSME+WGGATFDVCMRFL E+PW RL+L R+AMPN+L QMLLR SN VGY AYPDNL+EKF+ K AE G+DIFRIFDSLNW ++M++SIRTVRE+TNS+AEACICYTGD+   N  K+NLQYY+DLA++LE+ GAHIL IKDMAGLL P +A  LI+ LK+AV++PIHLHTHDTSS+Q ATYL+AI+AGVDV+DVA+SSMSGLTSQPNFNS+   L+GH R +  D+  LN++ NYWED+REFYYPFESELKAGTA+VYE+EIPGGQYSNLRPQARGLG+ED+F  +KENY I N+LFG +VKVTPSSKVVGDMA+FMTSN  T +DI+ KG   +FP+SV++ FKGD+GQP GGFP +L KIILK +  FTDRPN HL PVDF+KEF +FQ+EF  +  +LDFLSYKLYPKV+KD ++  +  G+VW +PT +F YG+K NEEI +EI
Sbjct:  567 EGYAKNHPNIFSMEVWGGATFDVCMRFLHENPWDRLKLFRQAMPNLLLQMLLRGSNGVGYKAYPDNLIEKFVEKTAETGIDIFRIFDSLNWVEAMKVSIRTVRERTNSLAEACICYTGDLYKENP-KYNLQYYLDLARQLEDEGAHILAIKDMAGLLKPRSARLLISELKKAVDLPIHLHTHDTSSLQCATYLEAIDAGVDVVDVAISSMSGLTSQPNFNSLLAALEGHEREHKFDLKSLNKYGNYWEDVREFYYPFESELKAGTAEVYEHEIPGGQYSNLRPQARGLGLEDQFARVKENYAIVNDLFGDLVKVTPSSKVVGDMALFMTSNNLTADDILQKGHTLSFPESVVSLFKGDLGQPPGGFPEQLTKIILKGEKPFTDRPNAHLEPVDFDKEFEQFQEEFDEYCDELDFLSYKLYPKVFKDLYDHWQHYGEVWRLPTLSFLYGLKNNEEIFVEI 1024          

HSP 2 Score: 531.561 bits (1368), Expect = 6.109e-170
Identity = 277/432 (64.12%), Postives = 332/432 (76.85%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKA---MPNILTQMLLRSSNA 429
            AATELKIRT+A++T+EDRYSLHRYKADEAYQIG D EPLKPYLDIEE+I +AK+ + DAIHPGYGFLSENV+ A+RCREEGI F+GP PE M +LGDKV AK IA  A +P+IEDS+  +     AL EA  IG+P+MVKAAAGGGGRGMRVVR +E+L +AF EA+ EA KAFG DT+FLEK+I+NPKHIEVQ++GD +GN+VHLYERDCSVQRRFQKVVEVAP+ +L QE KDKLY YA+ I ++V Y N GTVEFL+D++ENIYFIEVNPR+QVEHTITEE+TG+DIVR+QILIA G  LA P IFI  Q  V   G+AIQCR+TTEDP NNFQPDYGTIIAYR+A G GIRLD G+ Y GVTISPFFD    K       +  WG        R  +     R+R ++     + N+++  +  S  A
Sbjct:   26 AATELKIRTVAVFTYEDRYSLHRYKADEAYQIGADNEPLKPYLDIEELIAVAKRNEVDAIHPGYGFLSENVKLARRCREEGIVFVGPEPEVMEKLGDKVQAKKIAIKAQLPIIEDSREKLTSTDIALKEASNIGYPVMVKAAAGGGGRGMRVVRDDEQLKRAFDEARQEAGKAFGDDTVFLEKYIDNPKHIEVQIMGDNYGNIVHLYERDCSVQRRFQKVVEVAPSVTLSQEAKDKLYGYALAIAREVNYNNVGTVEFLVDSDENIYFIEVNPRIQVEHTITEEVTGVDIVRSQILIAKGYDLADPRIFIKDQSGVQCNGYAIQCRVTTEDPHNNFQPDYGTIIAYRSANGFGIRLDAGNCYAGVTISPFFDSLLVK-------VSSWGRTLKGASERLDRALSEFRIRGVKTNVGFLQNVISHPVFYSGQA 450          

BLAST of pyruvate carboxylase vs. nr
Match: gi|921286249|ref|WP_053222850.1| (pyruvate carboxylase [Roseivirga seohaensis] >gi|917717122|gb|KOF03485.1| pyruvate carboxylase [Roseivirga seohaensis subsp. aquiponti])

HSP 1 Score: 694.501 bits (1791), Expect = 0.000e+0
Identity = 312/457 (68.27%), Postives = 388/457 (84.90%), Query Frame = 0

Query:  375 FAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            F KNHPE+FS+E+WGGATFDV MRFL E+PW RL+L+R+A PN LTQMLLR SN VGYTAYPDNL+E+F+ K AE+GMD+FRIFDSLNW ++M++SI+TVRE+TNS+AEACICYTGD+++ N  K+NLQYY+D+AK LE+ GAHILCIKDMAGLL P AA KLITALKE+V +PIHLHTHDTSS+Q+ TY  AIEAGVD++DVA++SMSGLTSQPNFNSI   L+GH R    +++KLNE+SNYWED+RE+YYPFES LKAGTA+VY++EIPGGQYSNLRPQA  LG+E KFE +K NY I N+LFG +VKVTPSSKVVGDMA++MTSN  T EDIMT+G+  +FP+SV++ F+GD+GQ  GGFP EL KIILK + A+TDRPN HL PVDF+KEF +FQ +F  +  +LDFLSYKLYPKV++DF+E  +  G+V  +PT  FFYG+K+NEE+ ++I
Sbjct:  569 FTKNHPEIFSLEMWGGATFDVSMRFLNENPWDRLKLIREAAPNTLTQMLLRGSNGVGYTAYPDNLIERFVEKTAEHGMDVFRIFDSLNWIENMKVSIKTVRERTNSVAEACICYTGDVLDAN-SKYNLQYYIDMAKALEDEGAHILCIKDMAGLLKPRAATKLITALKESVEMPIHLHTHDTSSMQATTYFHAIEAGVDIVDVAIASMSGLTSQPNFNSIVACLEGHERDPKINLSKLNEYSNYWEDVREYYYPFESGLKAGTAEVYDHEIPGGQYSNLRPQATALGLEHKFEEVKRNYAIVNKLFGDIVKVTPSSKVVGDMAIYMTSNNLTVEDIMTRGESLSFPESVVSLFRGDLGQTPGGFPQELSKIILKGKTAYTDRPNAHLVPVDFDKEFKQFQIDFDEYCDELDFLSYKLYPKVFRDFYEHWQTYGEVRHLPTKAFFYGLKENEEVFVQI 1024          

HSP 2 Score: 546.969 bits (1408), Expect = 8.503e-176
Identity = 280/377 (74.27%), Postives = 318/377 (84.35%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAK 377
            AA+ELKIRT+A+YT+EDRYSLHRYKADEAYQIGE+EE LKPYLDI+EI+ LAK E  DAIHPGYGFLSENV F + C+E GI FIGP PE M +LGDKV AKI+AR A VP+IEDSQ  +N    AL EAK IGFP+MVKAAAGGGGRGMRVVR E +L KAF EAK+EA KAFG DT+FLEKFI+NPKHIEVQ++GD +G+LVHLYERDCSVQRR+QKVVEVAP+ +L Q T+DKLYEYA++I K+V Y N GTVEFL+DAEEN+YFIEVNPRVQVEHTITEEITGIDIVR+QI IA G PL+ P IFI  Q D+   GFAIQCRITTEDP+N F+PDYGTIIAYR+A G GIRLD G+ Y GVTISPFFD    K
Sbjct:   26 AASELKIRTVAMYTYEDRYSLHRYKADEAYQIGENEEALKPYLDIQEIMTLAKNEGVDAIHPGYGFLSENVHFVRACKEAGIIFIGPEPEVMEKLGDKVQAKIVARKAQVPLIEDSQESLNSFDIALKEAKRIGFPVMVKAAAGGGGRGMRVVRQEADLEKAFNEAKSEASKAFGDDTMFLEKFIDNPKHIEVQIMGDNYGHLVHLYERDCSVQRRYQKVVEVAPSINLSQTTRDKLYEYALRITKEVNYNNVGTVEFLVDAEENVYFIEVNPRVQVEHTITEEITGIDIVRSQIEIAQGYPLSDPRIFIKDQADIQCNGFAIQCRITTEDPENGFKPDYGTIIAYRSAGGFGIRLDAGNCYAGVTISPFFDSMLVK 402          

BLAST of pyruvate carboxylase vs. nr
Match: gi|1011387715|ref|WP_062300458.1| (pyruvate carboxylase [Roseivirga seohaensis] >gi|1005901526|gb|KYG85011.1| pyruvate carboxylase [Roseivirga seohaensis])

HSP 1 Score: 693.73 bits (1789), Expect = 0.000e+0
Identity = 312/457 (68.27%), Postives = 388/457 (84.90%), Query Frame = 0

Query:  375 FAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            F KNHPE+FS+E+WGGATFDV MRFL E+PW RL+L+R+A PN LTQMLLR SN VGYTAYPDNL+E+F+ K AE+GMD+FRIFDSLNW ++M++SI+TVRE+TNS+AEACICYTGD+++ N  K+NLQYY+D+AK LE+ GAHILCIKDMAGLL P AA KLITALKE+V +PIHLHTHDTSS+Q+ TY  AIEAGVD++DVA++SMSGLTSQPNFNSI   L+GH R    +++KLNE+SNYWED+RE+YYPFES LKAGTA+VY++EIPGGQYSNLRPQA  LG+E KFE +K NY I N+LFG +VKVTPSSKVVGDMA++MTSN  T EDIMT+G+  +FP+SV++ F+GD+GQ  GGFP EL KIILK + A+TDRPN HL PVDF+KEF +FQ +F  +  +LDFLSYKLYPKV++DF+E  +  G+V  +PT  FFYG+K+NEE+ ++I
Sbjct:  569 FTKNHPEIFSLEMWGGATFDVSMRFLNENPWDRLKLIREAAPNTLTQMLLRGSNGVGYTAYPDNLIERFVEKTAEHGMDVFRIFDSLNWIENMKVSIKTVRERTNSVAEACICYTGDVLDAN-SKYNLQYYIDMAKALEDEGAHILCIKDMAGLLKPRAATKLITALKESVEMPIHLHTHDTSSMQATTYFHAIEAGVDIVDVAIASMSGLTSQPNFNSIVACLEGHERDPKINLSKLNEYSNYWEDVREYYYPFESGLKAGTAEVYDHEIPGGQYSNLRPQATALGLEHKFEEVKRNYAIVNKLFGDIVKVTPSSKVVGDMAIYMTSNNLTVEDIMTRGESLSFPESVVSLFRGDLGQTPGGFPQELSKIILKGKTAYTDRPNAHLEPVDFDKEFKQFQIDFDEYCDELDFLSYKLYPKVFRDFYEHWQTYGEVRHLPTKAFFYGLKENEEVFVQI 1024          

HSP 2 Score: 545.814 bits (1405), Expect = 2.146e-175
Identity = 281/377 (74.54%), Postives = 317/377 (84.08%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAK 377
            AA+ELKIRT+A+YT+EDRYSLHRYKADEAYQIGE+EE LKPYLDI+EII LAK E  DAIHPGYGFLSENV F + C+E GI FIGP PE M +LGDKV AKI+A  A VP+IEDSQ  +N    AL EAK IGFPIMVKAAAGGGGRGMRVVR E +L KAF EAK+EA KAFG DT+FLEKFI+NPKHIEVQ++GD +G+LVHLYERDCSVQRR+QKVVEVAP+ +L Q T+DKLYEYA++I K+V Y N GTVEFL+DAEEN+YFIEVNPRVQVEHTITEEITGIDIVR+QI IA G PL+ P IFI  Q D+   GFAIQCRITTEDP+N F+PDYGTIIAYR+A G GIRLD G+ Y GVTISPFFD    K
Sbjct:   26 AASELKIRTVAMYTYEDRYSLHRYKADEAYQIGENEEALKPYLDIQEIITLAKNEGVDAIHPGYGFLSENVHFVRACKEAGIIFIGPEPEVMEKLGDKVQAKIVASKAQVPLIEDSQESLNSFDVALKEAKRIGFPIMVKAAAGGGGRGMRVVRQEADLEKAFNEAKSEASKAFGDDTMFLEKFIDNPKHIEVQIMGDNYGHLVHLYERDCSVQRRYQKVVEVAPSINLSQTTRDKLYEYALRITKEVNYNNVGTVEFLVDAEENVYFIEVNPRVQVEHTITEEITGIDIVRSQIEIAQGYPLSDPRIFIKDQADIQCNGFAIQCRITTEDPENGFKPDYGTIIAYRSAGGFGIRLDAGNCYAGVTISPFFDSMLVK 402          

BLAST of pyruvate carboxylase vs. nr
Match: gi|1277040235|gb|PIQ48463.1| (pyruvate carboxylase [Cytophagales bacterium CG12_big_fil_rev_8_21_14_0_65_40_12] >gi|1278016314|gb|PIW03767.1| pyruvate carboxylase [Cytophagales bacterium CG17_big_fil_post_rev_8_21_14_2_50_40_13])

HSP 1 Score: 692.96 bits (1787), Expect = 0.000e+0
Identity = 317/459 (69.06%), Postives = 385/459 (83.88%), Query Frame = 0

Query:  373 KSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            + F KNHPE+FS+E+WGGATFDV MRFL E+PW RLRL+R+A PN+LTQMLLR SN VGY AYPDNL+EKFI K+AENGMD+FRIFDSLNW ++M++SI+TVRE+TNS+AEACICYTG+++  N  KFNLQYY D+AK LE+ GAHILCIKDMAGLL P AA  LI ALKEAVN+PIHLHTHDTSS+Q+ TY +AI AGVD++DVA++SMSGLTSQPNFNS+   L GH R +N D+ KLNE+SNYWED+REFYYPFES LKAGTAQVY++EIPGGQYSNLRPQA  LG+E KFE +K+NY I N+LFGG+VKVTPSSKVVGDMA++MTSN  T EDIMT+G+  +FP+SV++ F+GD+GQ  GGFP ++ KIILK Q  FTDRPN HL P+DFEKEF +FQK+F     +LDF+SY LYPKV+K+FH      G+V  +PT  FFYG+K+NEEI ++I
Sbjct:  567 EGFTKNHPEIFSLEMWGGATFDVSMRFLHENPWERLRLIRQAAPNVLTQMLLRGSNGVGYKAYPDNLIEKFIEKSAENGMDVFRIFDSLNWIENMKVSIKTVRERTNSVAEACICYTGNVLEAN-SKFNLQYYTDMAKALEDEGAHILCIKDMAGLLQPRAAAVLIPALKEAVNLPIHLHTHDTSSMQATTYFEAINAGVDIVDVAIASMSGLTSQPNFNSLLASLNGHERESNIDLGKLNEYSNYWEDVREFYYPFESGLKAGTAQVYDHEIPGGQYSNLRPQATALGLEHKFEEVKKNYAIVNKLFGGLVKVTPSSKVVGDMAIYMTSNNLTVEDIMTRGETLSFPESVVSLFRGDLGQMPGGFPQDISKIILKGQKPFTDRPNAHLTPIDFEKEFAQFQKDFDEFCDELDFISYLLYPKVFKEFHMHWLNYGEVRHLPTKAFFYGLKENEEIFVQI 1024          

HSP 2 Score: 550.051 bits (1416), Expect = 4.850e-177
Identity = 284/377 (75.33%), Postives = 319/377 (84.62%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAK 377
            AA+ELKIRT+A+YT+EDRYSLHRYKADEAYQIGE++EPLKPYLDIEEII LAK E  DAIHPGYGFLSENV F + C+E GI FIGP PE M +LGDKV AKI+A+ A VP+IEDS   +   + AL EAK IGFP+MVKAAAGGGGRGMRVVR E +L K+F EAK E+ KAFG DT+FLEKFI+NPKHIEVQ++GD +GNLVHLYERDCSVQRR+QKVVEVAP+ +LKQETKDKLYEYA+KI K+V Y N GTVEFL+DA+ENIYFIEVNPR+QVEHTITEEITGIDIVRTQI IA G PL  P IFI  QEDV   GFAIQCRITTEDP+N F+PDYGTIIAYR+A G GIRLD G+ Y GVTISPFFD    K
Sbjct:   26 AASELKIRTVAMYTYEDRYSLHRYKADEAYQIGENDEPLKPYLDIEEIITLAKNEGVDAIHPGYGFLSENVRFVRACKEAGIIFIGPEPEVMERLGDKVQAKIVAKNAEVPLIEDSHELLTTSSIALKEAKRIGFPVMVKAAAGGGGRGMRVVREEADLEKSFNEAKGESLKAFGDDTMFLEKFIDNPKHIEVQIMGDNYGNLVHLYERDCSVQRRYQKVVEVAPSVNLKQETKDKLYEYALKITKEVNYNNVGTVEFLVDADENIYFIEVNPRIQVEHTITEEITGIDIVRTQIEIAQGYPLDDPRIFIKSQEDVQCNGFAIQCRITTEDPENGFKPDYGTIIAYRSAGGFGIRLDAGNCYAGVTISPFFDSMLVK 402          

BLAST of pyruvate carboxylase vs. nr
Match: gi|655911193|ref|WP_028981414.1| (pyruvate carboxylase [Sporocytophaga myxococcoides])

HSP 1 Score: 692.96 bits (1787), Expect = 0.000e+0
Identity = 310/459 (67.54%), Postives = 394/459 (85.84%), Query Frame = 0

Query:  373 KSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLRLLRKAMPNILTQMLLRSSNAVGYTAYPDNLVEKFIIKAAENGMDIFRIFDSLNWTKSMELSIRTVREKTNSIAEACICYTGDIMNPNKQKFNLQYYVDLAKELEEMGAHILCIKDMAGLLTPFAAEKLITALKEAVNIPIHLHTHDTSSIQSATYLKAIEAGVDVIDVALSSMSGLTSQPNFNSIANMLQGHTRGNNTDITKLNEFSNYWEDIREFYYPFESELKAGTAQVYENEIPGGQYSNLRPQARGLGIEDKFETIKENYKIANELFGGVVKVTPSSKVVGDMAMFMTSNGYTKEDIMTKGKDFAFPDSVINFFKGDIGQPYGGFPTELQKIILKDQIAFTDRPNEHLAPVDFEKEFIEFQKEFGNHKTDLDFLSYKLYPKVYKDFHEFNEVNGDVWVIPTTTFFYGMKQNEEILIEI 831
            +SFAKNHP+VFSME+WGGATFDV MRFL EDPW RL+L+R+A+PNIL QML+R SNAVGY AYPDNL+EKFI K  ENG+DIFRIFDSLNW ++M++SI+ V+E+T  IAEA +CY+GDI++P  +K+ LQYY+D+A++LE++GAHILCIKDMAGLL P++AE LI  LK+AV+IPIHLHTHDTS+IQ+ATYLKA+EAGVD+IDVA++SMSGLTSQPNFNSI  ML+GH R    ++  LN++S+YWED+REFYYPFES+LKAGTA+VYENEIPGGQYSNLRPQA  LG+ +KFE +K+NY + N++FG ++KVTPSSKVVGDMA+FMTSN  T++DIM +G+  +FP+SV++ FKG+IGQ YGGFP +LQ IILK +  FT+RPN+HLAPVDFEKEF  FQ++FGN  + LDFLSYK+YPKV++ + +  E  GDV  IPT  FFYG+K N+EIL+++
Sbjct:  566 ESFAKNHPQVFSMEVWGGATFDVAMRFLHEDPWQRLQLIREAVPNILLQMLIRGSNAVGYKAYPDNLIEKFIEKTWENGVDIFRIFDSLNWVEAMKVSIKAVKERTGGIAEAAVCYSGDILDPENKKYTLQYYLDMARQLEDLGAHILCIKDMAGLLKPYSAELLIKELKKAVSIPIHLHTHDTSAIQAATYLKAVEAGVDIIDVAIASMSGLTSQPNFNSIVTMLKGHERELKFNMPALNQYSSYWEDVREFYYPFESDLKAGTAEVYENEIPGGQYSNLRPQAESLGLGEKFEKVKQNYALVNKMFGDIIKVTPSSKVVGDMALFMTSNNLTEKDIMERGQYLSFPESVVSLFKGEIGQAYGGFPEKLQSIILKGEKPFTERPNDHLAPVDFEKEFEAFQQQFGNTASFLDFLSYKMYPKVFEGYAKHREQYGDVSNIPTLAFFYGLKNNQEILVKL 1024          

HSP 2 Score: 542.732 bits (1397), Expect = 3.921e-174
Identity = 277/409 (67.73%), Postives = 323/409 (78.97%), Query Frame = 0

Query:    1 AATELKIRTIALYTFEDRYSLHRYKADEAYQIGEDEEPLKPYLDIEEIILLAKKEKADAIHPGYGFLSENVEFAKRCREEGIKFIGPSPESMAQLGDKVAAKIIARAANVPVIEDSQLDMNEVATALSEAKIIGFPIMVKAAAGGGGRGMRVVRTEEELSKAFTEAKNEAKKAFGIDTIFLEKFIENPKHIEVQLLGDEHGNLVHLYERDCSVQRRFQKVVEVAPAPSLKQETKDKLYEYAIKIGKQVGYYNAGTVEFLLDAEENIYFIEVNPRVQVEHTITEEITGIDIVRTQILIASGLPLAHPSIFITKQEDVNIQGFAIQCRITTEDPQNNFQPDYGTIIAYRNAAGMGIRLDEGSSYPGVTISPFFDKSFAKNHPEVFSMEIWGGATFDVCMRFLKEDPWMRLR 409
            AA+ELKI+T+A+YT+EDRYSLHRYKADEAYQIG D EPLKPYLDIE+II LAK+   DAIHPGYGFLSENV  A+RC+EEGI FIGP PE M  LGDKV+AK IA +A VP+IEDS+  +  +  A  EA  IG+P+M+KAAAGGGGRGMRV+R E +L +A+ EA+ EA KAFG DT+FLEKFI+ PKHIEVQL+GD HGN+VHLYERDCSVQRRFQKVVEVAP P LK+ETK ++Y YA+ I + V Y N GTVEFL+D +E +YFIEVNPR+QVEHTITEEITGIDIVR+QILIA G  L+   I+I++QEDV   GFAIQCR+TTEDP N F+PDYGTIIAYRNAAG GIRLDEGSSYPGV ISPFFD    K      +M  WG      C R  +     R+R
Sbjct:   25 AASELKIKTVAIYTYEDRYSLHRYKADEAYQIGRDNEPLKPYLDIEQIIQLAKRHSVDAIHPGYGFLSENVNLARRCKEEGIIFIGPEPEVMEALGDKVSAKEIAISAGVPIIEDSKEGLLSLDIARKEAHRIGYPLMMKAAAGGGGRGMRVIRDESQLERAYNEARAEAMKAFGDDTVFLEKFIDRPKHIEVQLMGDNHGNIVHLYERDCSVQRRFQKVVEVAPCPVLKEETKQQIYNYALTIARHVKYNNVGTVEFLVDQDEKVYFIEVNPRIQVEHTITEEITGIDIVRSQILIAKGHKLSDTCIYISRQEDVKCNGFAIQCRVTTEDPANGFKPDYGTIIAYRNAAGFGIRLDEGSSYPGVKISPFFDSLLVKVSASGRTM--WGA-----CERLNRTLREFRIR 426