BLAST of unknown vs. L. salmonis genes
Match: EMLSAG00000008038 (supercontig:LSalAtl2s:LSalAtl2s478:620550:622126:1 gene:EMLSAG00000008038 transcript:EMLSAT00000008038 description:"maker-LSalAtl2s478-augustus-gene-6.21")

HSP 1 Score: 169.474 bits (428), Expect = 2.537e-49
Identity = 101/272 (37.13%), Postives = 154/272 (56.62%), Query Frame = 0

Query:   48 ELRDELAAVEAILGRAVELAAG-SGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRDLL-QDVR---LEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSVDRAI-------ETPIP 307
            E+ +E+ A+EA L   + +  G  GS         ++P TASD ++ +V+LTL+L  P  YP   P I I  PRGL ++ +  LE  L +KC D  G+P++Y ++++  EFLTE+NVP+  C+VCL D+   DSF+KT C+HYFH+LC G Y+ N I+  +    +        ++  R LR  CPVCR++L QDV       +L S PP ++  E   F  T++++ELQ  M+ L ++Q   G+II  EA +     +I       E P+P
Sbjct:   11 EVIEEVEALEATLLEGIRIRRGIEGSERPTDLELVMTPLTASDEERAFVSLTLKLSIPLGYPRERPSIVIAHPRGLGESGISSLEKGLAKKCRDNLGDPILYQLVEYTQEFLTESNVPACSCAVCLCDLKKEDSFIKTPCYHYFHSLCYGSYIQNEISNRKAEEEEK------QEQTTRDLR--CPVCREILVQDVLNYDFSHFLKSPPPVVQVPES--FTLTEDLKELQNSMKNLFEKQKLNGAIIDIEAENSMTVLSIGENTERNELPLP 272          

BLAST of unknown vs. SwissProt
Match: gi|68053269|sp|Q5E9N3.1|RNF25_BOVIN (RecName: Full=E3 ubiquitin-protein ligase RNF25; AltName: Full=RING finger protein 25; AltName: Full=RING-type E3 ubiquitin transferase RNF25)

HSP 1 Score: 140.969 bits (354), Expect = 1.867e-36
Identity = 90/266 (33.83%), Postives = 134/266 (50.38%), Query Frame = 0

Query:   49 LRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQ-------LRLLCPVCRD-LLQDVRLEAWLASSPPSLERDEDGKFIKTD-EIEELQIRMQALLQRQLEKGSIIHSEAASGSVDRAIETP 305
            L  E+  +E+I    +++  G+G ++ W     L P TA D D QYV  TL L  P +YP   PQI I  PRGLSD ++  +   L    E   G  ++Y +I+   E LT+NN+P  +C +CLY   + ++F KT C+HYF   CL +Y+ ++    E  L+      G  +EQERQ       + + CPVCR+ L+ D      LAS   + E  +  +  + D E    Q   + L QRQ E+G II  EA       +++ P
Sbjct:   16 LPSEVEVLESIYLDELQVVKGNGRSSPWEIYITLHPATAEDQDSQYVCFTLVLQVPTQYPHEVPQISIRNPRGLSDEQIHKISQALSHVAEAGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFRCHCLARYIQHM----EHELQAQ----GREREQERQHAAPEQAVGVQCPVCREPLVYD------LASLKAAPEPQQPMELYQPDAESLRQQEERKRLYQRQQERGGIIDLEAERNRYFISLQQP 267          

BLAST of unknown vs. SwissProt
Match: gi|21362899|sp|Q96BH1.1|RNF25_HUMAN (RecName: Full=E3 ubiquitin-protein ligase RNF25; AltName: Full=RING finger protein 25; AltName: Full=RING-type E3 ubiquitin transferase RNF25)

HSP 1 Score: 137.502 bits (345), Expect = 4.057e-35
Identity = 81/258 (31.40%), Postives = 131/258 (50.78%), Query Frame = 0

Query:   49 LRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRD-LLQDVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSVDRAIETP 305
            L  E+  +E+I    +++  G+G  + W     L P TA D D QYV  TL L  P  YP   PQI I  PRGLSD ++  +   L    +   G  ++Y +I+   E LT+NN+P  +C +CLY   + ++F KT C+HYFH  CL +Y+ ++    +   ++       +  +++ + + CPVCR+ L+ D+   A L ++P   +  E   +  + E    Q   + L QRQ E+G II  EA       +++ P
Sbjct:   16 LPSEVEVLESIYLDELQVIKGNGRTSPWEIYITLHPATAEDQDSQYVCFTLVLQVPAEYPHEVPQISIRNPRGLSDEQIHTILQVLGHVAKAGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELKAQGQEQEQERQHATTKQKAVGVQCPVCREPLVYDL---ASLKAAPEPQQPME--LYQPSAESLRQQEERKRLYQRQQERGGIIDLEAERNRYFISLQQP 268          

BLAST of unknown vs. SwissProt
Match: gi|21362901|sp|Q9QZR0.2|RNF25_MOUSE (RecName: Full=E3 ubiquitin-protein ligase RNF25; AltName: Full=RING finger protein 25; AltName: Full=RING finger protein AO7; AltName: Full=RING-type E3 ubiquitin transferase RNF25)

HSP 1 Score: 134.806 bits (338), Expect = 4.067e-34
Identity = 88/269 (32.71%), Postives = 135/269 (50.19%), Query Frame = 0

Query:   49 LRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNV---INTHEEFLRDNPPIPGVSQEQERQ--------LRLLCPVCRD-LLQDVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSVDRAIETP 305
            L  E+  +E+I    +++  G+G +  W     L P TA   D Q+V  TL L  P +YP   PQI I  PRGLSD ++  +   L    ++  G  ++Y +I+   E LT+NN+P  +C +CLY   + ++F KT C+HYFH  CL +Y+ ++   + T E             QEQERQ        + + CPVCR+ L+ D+   A L ++P   +  E   +  + E    Q  ++ L QRQ EKG II  EA       +++ P
Sbjct:   16 LPSEVEVLESIYLDELQVMKGNGRSP-WEIFITLHPATAEVQDSQFVCFTLVLRIPVQYPHEVPQISIRNPRGLSDEQIHKISQALGHVAKEGLGTAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELTTQE-------------QEQERQHVVTKQKAVGVQCPVCREPLVYDL---ASLKAAPEPQQPME--LYQPSAESLRQQEELKLLYQRQQEKGGIIDLEAERNRYFISLQQP 265          

BLAST of unknown vs. SwissProt
Match: gi|61213023|sp|Q9QZ05.2|E2AK4_MOUSE (RecName: Full=eIF-2-alpha kinase GCN2; AltName: Full=Eukaryotic translation initiation factor 2-alpha kinase 4; AltName: Full=GCN2-like protein; Short=mGCN2)

HSP 1 Score: 55.8398 bits (133), Expect = 4.214e-7
Identity = 25/71 (35.21%), Postives = 43/71 (60.56%), Query Frame = 0

Query:   94 YVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVP 164
            YV + LQ+  P  YP+  P+I++   +GLS+  +  L+  L++  +  CGE +I+ +  HV  FL+E+N P
Sbjct:   70 YVQVELQVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSHLEELAKKQCGEVMIFELAHHVQSFLSEHNKP 140          

BLAST of unknown vs. nr
Match: gi|751476847|ref|XP_011193923.1| (PREDICTED: E3 ubiquitin-protein ligase RNF25 [Zeugodacus cucurbitae])

HSP 1 Score: 178.718 bits (452), Expect = 2.146e-48
Identity = 103/256 (40.23%), Postives = 157/256 (61.33%), Query Frame = 0

Query:   49 LRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRDLLQDVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSVDRAIET 304
            LRDE+ ++EAIL   V +   S S    L    + P    D D+QYV +TLQ+     YPE+SPQ ++LRPRGL D +L+ ++H  ++K ++  G PV++ +I+ V E LT +N+PS +C VCLY  +D D F +T CFHY H+ CLG+++N +  T+++   +   +P   Q+     + LCPVCR+ ++D   ++   + PPS E ++  +FI T E+ +LQ RM AL  +Q  KG II  +A SGSV  +IET
Sbjct:    4 LRDEVESLEAILMDDVIITRNSESGVPELIETTVFPQVG-DEDEQYVCVTLQVIPSSDYPETSPQYKLLRPRGLDDDRLEKIKHACNEKLQESIGFPVVFDLIEVVREHLTGSNLPSGQCVVCLYGFSDGDEFTRTECFHYLHSYCLGRHLNALRRTYKD---EYDKLPSWLQKTVDPFQALCPVCREHIKDEE-DSLRCAMPPS-ELEQAPEFILTRELRDLQHRMSALFLKQKNKGGIIDVDAESGSV-ISIET 252          

BLAST of unknown vs. nr
Match: gi|751771506|ref|XP_011205636.1| (PREDICTED: E3 ubiquitin-protein ligase RNF25 [Bactrocera dorsalis])

HSP 1 Score: 174.096 bits (440), Expect = 8.785e-47
Identity = 97/250 (38.80%), Postives = 147/250 (58.80%), Query Frame = 0

Query:   49 LRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRDLLQDVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSV 298
            L DE+ ++EAIL   V++   S S    L    + P    DA+ QY+ +TLQ+     YPE+SP  ++LRPRGL D +L+ ++H  ++K E+  G PV++ +I+ V E LT +N+PS +C VCLY  +D D F +T CFHY H+ CLG+++N +  T++E   +   +P   Q+     + LCPVCR+ ++D       A  P  LE+  + K   T E+ +LQ RM AL  +Q  KG II  +A SG+V
Sbjct:    4 LIDEVESLEAILMDDVKITRNSESGLPELIETTVFPQVGDDAE-QYICVTLQVSPSSDYPETSPHYKLLRPRGLDDERLEEIKHACNEKLEESVGFPVVFDLIEVVREHLTGSNLPSGQCVVCLYGFSDGDEFTRTECFHYLHSYCLGRHLNALRRTYKE---EYDKLPSWLQKTVDPFQALCPVCREPIKDEEDSLRCAMPPSELEKAPEFKL--THELRDLQQRMSALFLKQKNKGGIIDVDAESGTV 247          

BLAST of unknown vs. nr
Match: gi|1130261992|ref|XP_019769918.1| (PREDICTED: E3 ubiquitin-protein ligase RNF25 [Dendroctonus ponderosae] >gi|332373980|gb|AEE62131.1| unknown [Dendroctonus ponderosae] >gi|478250944|gb|ENN71428.1| hypothetical protein YQE_11847, partial [Dendroctonus ponderosae] >gi|546682688|gb|ERL92600.1| hypothetical protein D910_09913 [Dendroctonus ponderosae])

HSP 1 Score: 169.859 bits (429), Expect = 2.988e-46
Identity = 95/256 (37.11%), Postives = 146/256 (57.03%), Query Frame = 0

Query:   44 SMTAELRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRDLLQ-DVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSV 298
            S    +++EL A+EAI    + +          + +  + P TA++ DKQYV +TL++  P+ YP+S P I +  PRGL D+ +  L +E+ +KC++  G+PVIY +I+ + E LTE+N+P+ +C+VCLY  +D DSF KT CFHYFH  CL    N++I T + F  +   +P   +   +  +  CPVCRD +  DV     L ++ P  E D+  KF  T +++ LQ +MQ L   Q  KG II  EA    +
Sbjct:    2 SQLERVQEELEALEAIFMDDISIIYNERGCVDLVKSV-IFPSTANEVDKQYVCVTLEVKLPEDYPDSKPIIALKSPRGLDDSTIDHLHNEIQKKCDEYLGQPVIYELIELIRENLTESNLPTCQCAVCLYGFSDGDSFTKTQCFHYFHNYCLA---NHLIITGKHFKEEQEKLPA-WKRSTKAFQATCPVCRDAIYCDVD---ELKTALPPRELDDAQKFEVTHDLKVLQQKMQQLFNYQKSKGGIIDIEAEENKL 249          

BLAST of unknown vs. nr
Match: gi|189242493|ref|XP_972306.2| (PREDICTED: E3 ubiquitin-protein ligase RNF25 [Tribolium castaneum] >gi|1004394187|gb|EFA12922.2| E3 ubiquitin-protein ligase RNF25-like Protein [Tribolium castaneum])

HSP 1 Score: 165.236 bits (417), Expect = 1.282e-44
Identity = 95/271 (35.06%), Postives = 150/271 (55.35%), Query Frame = 0

Query:   44 SMTAELRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRDLLQ-DVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSVDRAIETPIPDAKNSP 313
            S+   +++E+ A+EAIL   + ++ G       + +  + P TA D DKQYV +TL++  P  YP+  P +Q+  PRGL D  L  L   +  KC +  G+PVIY +I+ + E LTE+N+P+  C+VCLY  ++ DSF KT CFHYFH+ CL  +   +I T + F+ +   +P   Q+     +  CPVCR+ +  DV+    L ++ P  E ++   F  T E++ LQ +M+ L   Q  +G II  EA    +    E+    ++NSP
Sbjct:    2 SLNERVQEEIEALEAILMDDISVSYGDNGCPELVKS-TIFPSTADDTDKQYVCVTLEVKLPGDYPDCEPLVQLRNPRGLDDTTLNHLYRAIKDKCNEFIGQPVIYELIELIRENLTESNLPTCHCAVCLYGFSEGDSFTKTQCFHYFHSYCLAMH---LITTEKHFIEEQEKLP-TWQKSTTGFQASCPVCREPINCDVQ---ELKNALPPRELEKAQNFQVTKELQMLQAQMRQLFIYQKSRGGIIDVEAEENKLLLITES----SENSP 260          

BLAST of unknown vs. nr
Match: gi|929376996|ref|XP_014098702.1| (PREDICTED: E3 ubiquitin-protein ligase RNF25 isoform X1 [Bactrocera oleae])

HSP 1 Score: 168.318 bits (425), Expect = 3.837e-44
Identity = 93/250 (37.20%), Postives = 146/250 (58.40%), Query Frame = 0

Query:   49 LRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRDLLQDVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSV 298
            L+DE+ ++EAIL   V++     S    L    + P    +A+ QY+ +TLQ+     YPE+SP  ++LRPRGL D +L+ ++H  ++K ++  G PV++ +I+ V E LT +N+PS +C VCLY  +D D F +T CFHY H+ CLG+++N +  T+ E   +   +P   Q+     + LCPVCR+ ++D       A  P  LE+  + K   T E+++LQ RM  L  +Q  KG II  +A SG+V
Sbjct:   47 LKDEVESLEAILMEDVKITKNLESGLPELIETTVFPQVGDEAE-QYICVTLQVSPSSNYPETSPHYKLLRPRGLDDERLEEIKHACNEKLQESVGFPVVFDLIEVVREHLTGSNLPSGQCVVCLYGFSDGDEFTRTECFHYLHSYCLGRHLNALRRTYTE---EYDKLPSWLQKTVDPFQALCPVCREPIKDEEDSLRCAMPPSELEKAPEFKL--TRELQDLQHRMSTLFLKQKNKGGIIDVDAESGTV 290          

BLAST of unknown vs. nr
Match: gi|1285032319|ref|XP_023028541.1| (E3 ubiquitin-protein ligase RNF25 [Leptinotarsa decemlineata])

HSP 1 Score: 164.466 bits (415), Expect = 4.515e-44
Identity = 93/251 (37.05%), Postives = 145/251 (57.77%), Query Frame = 0

Query:   44 SMTAELRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRDLLQ-DVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEA 293
            S++  +++EL A+EAI    + +     S    +    + P TA++ DKQYV +TL++  P+ YP+  P +Q+  PRGL D+ +  L   + +KC +  G+ V+Y +I+ + E LTE+N+P+ +C+VCLY  +D DSF KT C+HYFH+ CL    N++I T   F  +   +P   Q+  +  +  CPVCR+ +  DV+ E  LA  P  LE  ++  F  T E+ ELQ  M+ L   Q +KG II  EA
Sbjct:    4 SLSERVQEELEALEAIFMDDISITYDEKSRPVLVKT-TIFPSTANEIDKQYVCVTLEVRLPEDYPDREPSVQLRNPRGLDDSTINNLYKSIKEKCTEFIGQAVMYELIELIRESLTESNLPTCQCAVCLYGFSDGDSFTKTQCYHYFHSYCLA---NHLITTKRHFQEEQDKLPA-WQKSTKGYQATCPVCRESINCDVK-ELRLALPPKDLENAQN--FQVTRELMELQENMRQLFIYQKKKGGIIDIEA 246          

BLAST of unknown vs. nr
Match: gi|1048057167|ref|XP_017461867.1| (PREDICTED: E3 ubiquitin-protein ligase RNF25, partial [Rhagoletis zephyria])

HSP 1 Score: 166.007 bits (419), Expect = 1.170e-43
Identity = 95/249 (38.15%), Postives = 150/249 (60.24%), Query Frame = 0

Query:   50 RDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRDLLQDVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSV 298
            RDE+ ++EAIL   V++     S A  L    + P    D  +QYV +TLQ+     YPE+SP+ ++LRPRGL DA+L+ ++   ++K  +  G PV++ +I+ V E LT +N+PS +C VCLY  +D D F +T CFHY H+ CLG+++N +  T++E   +   +P   Q+     + LCPVCR+ ++D + ++   + PPS E     +F  T E+ +LQ RM AL  +Q ++G II  +A SG+V
Sbjct:    1 RDEVESLEAILMDDVQITRNPESDAPELIETTVFPQVG-DEVEQYVCVTLQVIPTVDYPETSPEYKLLRPRGLDDARLEAIKRACNEKLRESIGFPVVFDLIEVVREHLTGSNLPSGQCVVCLYGFSDGDEFTRTECFHYLHSYCLGRHLNALRRTYKE---EYDKLPSWLQKTSDPFQALCPVCREPIKD-QEDSLRCAMPPS-ELQNAPEFKLTRELRDLQQRMSALFLKQKKRGGIIDVDAESGTV 243          

BLAST of unknown vs. nr
Match: gi|1098675397|ref|XP_018797996.1| (PREDICTED: E3 ubiquitin-protein ligase RNF25 [Bactrocera latifrons])

HSP 1 Score: 165.236 bits (417), Expect = 2.244e-43
Identity = 97/251 (38.65%), Postives = 149/251 (59.36%), Query Frame = 0

Query:   49 LRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTH-EEFLRDNPPIPGVSQEQERQLRLLCPVCRDLLQDVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSV 298
            L DE+ ++EAIL   V++     S    L    + P    DA+ QY+ +TLQ+     YPE+SP  ++LRPRGL D +L+ ++   + K E+  G PV++ +I+ V E LT +N+PS +C VCLY  +D D F +T CFHY H+ CLG+++N +  T+ EEF +    +P   Q+     + LCPVCR+ ++D   ++   + PPS E ++  +F  T E+ +LQ RM AL  +Q  KG II  +A SG+V
Sbjct:    4 LIDEVESLEAILMDDVKITRNLESGLPELIETTVFPQVGDDAE-QYICVTLQVSPSSDYPETSPHYKLLRPRGLDDERLEEIKLACNGKLEESVGFPVVFDLIEVVREHLTGSNLPSGQCVVCLYGFSDGDEFTRTECFHYLHSYCLGRHLNALRRTYKEEFDK----LPTWLQKTVDPFQALCPVCREPIKDEE-DSLRCAMPPS-ELEKAPEFQLTHELRDLQQRMSALFLKQKNKGGIIDVDAESGTV 247          

BLAST of unknown vs. nr
Match: gi|1060204301|ref|XP_017835885.1| (PREDICTED: E3 ubiquitin-protein ligase RNF25 [Drosophila busckii] >gi|924555906|gb|ALC41179.1| CG13344 [Drosophila busckii])

HSP 1 Score: 162.925 bits (411), Expect = 7.208e-43
Identity = 91/256 (35.55%), Postives = 150/256 (58.59%), Query Frame = 0

Query:   49 LRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRDLLQDVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSVDRAIET 304
            L +EL ++EAIL   V +  G       +    + P T  + D++Y+ +TL +  P  YP+ SP  ++LRPRGL DA+++ + +  + K ++  G PV++ +I+ V E LT +N+PS +C +CLY   D D F +T CFHY H+ CL +++N +  T++E   D   +PG  Q+  +  + LCPVCR+ + D   ++   + PPS E +   +F+ T E+ E+Q  M  L  +Q  +G+II  EA SG++  +IET
Sbjct:    4 LSEELESLEAILMDDVRIVRGPNDVVESIET-TVQPLTGEEDDQKYICVTLAVLPPAGYPDVSPNFKLLRPRGLDDARIEDIRNACNAKIKESLGFPVVFDLIEVVREHLTGSNLPSGQCVICLYGFCDGDEFTRTECFHYLHSYCLARHLNALRRTYQE---DFDKLPGWLQKSAKPFQALCPVCREHIND-ETDSLKCAMPPS-ELENAPEFMVTQELREMQELMSQLYLQQKSRGAIIDVEAESGTI-ISIET 252          

BLAST of unknown vs. nr
Match: gi|498973204|ref|XP_004527503.1| (E3 ubiquitin-protein ligase RNF25 [Ceratitis capitata])

HSP 1 Score: 164.081 bits (414), Expect = 7.346e-43
Identity = 97/256 (37.89%), Postives = 150/256 (58.59%), Query Frame = 0

Query:   49 LRDELAAVEAILGRAVELAAGSGSAASWLGAWPLSPHTASDADKQYVTLTLQLGFPDRYPESSPQIQILRPRGLSDAKLKFLEHELDQKCEDMCGEPVIYAIIDHVCEFLTENNVPSSECSVCLYDITDADSFVKTACFHYFHALCLGKYVNNVINTHEEFLRDNPPIPGVSQEQERQLRLLCPVCRDLLQDVRLEAWLASSPPSLERDEDGKFIKTDEIEELQIRMQALLQRQLEKGSIIHSEAASGSVDRAIET 304
            LRDE+ ++EAIL   V++     +    L    + P    +A+ QYV +TLQ+     YPE SPQ ++LRPRGL D +L+ ++   ++K  +  G PV++ +I+ V E LT +N+PS +C VCLY  +D D F +T CFHY H+ CLG+++N +  T++E   +   +P   ++     + LCPVCR+ ++D   E  L  + P LE +   +F  T E+ +LQ RM AL  +Q  KG II  +A S +V  +IET
Sbjct:    4 LRDEVDSLEAILMDDVKITKNLENGLPELIETTVFPQVGDEAE-QYVCVTLQVIPTTDYPERSPQFKLLRPRGLDDTRLEEIKCACNEKLRESIGFPVVFDLIEVVREHLTGSNLPSGQCVVCLYGFSDGDDFTRTECFHYLHSYCLGRHLNALRRTYKE---EYDKLPSWLKKTADPFQALCPVCREPIKDE--EDSLRCAMPPLELENAPEFKLTRELRDLQHRMSALFLKQKHKGGIIDVDAESANV-ISIET 252