BLAST of conserved unknown protein vs. L. salmonis genes
Match: EMLSAG00000003161 (supercontig:LSalAtl2s:LSalAtl2s1765:94718:97227:1 gene:EMLSAG00000003161 transcript:EMLSAT00000003161 description:"augustus_masked-LSalAtl2s1765-processed-gene-0.1")

HSP 1 Score: 244.588 bits (623), Expect = 6.714e-75
Identity = 132/401 (32.92%), Postives = 238/401 (59.35%), Query Frame = 0

Query:  185 SMKTEELWTRLRDKYVTKAMEEAFDFVTNIIKNLRDFGGIDDEEFDSPLFVLLALIKTAQDEFDQAWINSLPKTSSVDSITFDLHRRVSSHALQVYDASGEEDPKKQAQIMKIPSEKDIVYTYFKDTGD--HVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEF--LDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVLVRYDDDETANNLKRIQDEIMRIKQDRFPFLEHPGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDNC 581
            S   ++LW  +++  ++  ++EA + +  II+N+++   ID   F +PLF+L+AL     D F + ++N +   S + S  F   +++  +A+++Y AS        A+IM I     ++Y  + D+ +  H PKF++F   +  ++ L IRGT S+TDV +D++C D +    +G+AH GI  G+ KV+++   ++ ++LE   R +L+LTGHSLG G +EL+TM +++   +   +D +++CIA+APPPV+R+D ++P ++ +AI+I+IN  D VP + L ++A  ++++R +D L +   + F +L+   ++ T  N++ I++ I    QD FP+LEHPG I Y  K K         + +  +  Q S  F+R++ L + MI++HL   Y  AFDN 
Sbjct:   58 SNNMDQLWVAMKNAVLSPNVQEALNTIDEIIENVKEVDKID---FKTPLFLLVALFYKVAD-FKEEFLNDI---SDLKSNEFFEAKKIGRYAVRIYSASLLVSKAVIAKIMDI-ERSSLIYVSWMDSAEDTHHPKFLIFETDD--TVFLVIRGTFSVTDVFLDLVCSDRKVDKFEGYAHLGIYEGAQKVMEKSMPIISKSLESGNRKKLVLTGHSLGGGASELITMELIQQFEKEGKDDIDIKCIALAPPPVFRSDADIPHKIKNAIKIFINTSDIVPSLSLATVALFISILRQLDNLNLTMENIFYILMGSKNEVTEKNVQNIKEVIQSANQDSFPYLEHPGNIYYFSKTKDG-------TNRTFIKNQVSEYFSRKIRLFDGMIVNHLHGNYEVAFDNV 441          

BLAST of conserved unknown protein vs. L. salmonis genes
Match: EMLSAG00000009318 (supercontig:LSalAtl2s:LSalAtl2s595:47796:52338:1 gene:EMLSAG00000009318 transcript:EMLSAT00000009318 description:"maker-LSalAtl2s595-augustus-gene-0.25")

HSP 1 Score: 118.627 bits (296), Expect = 9.814e-29
Identity = 85/300 (28.33%), Postives = 144/300 (48.00%), Query Frame = 0

Query:  214 IIKNLRDFGGIDDEEFDS---PLFVLLALIKTAQDEFDQAWINSLPKTSSVDSI------TFDLHRRVSSHALQVYDASGEEDPKKQAQI-MKIPSEKDIVYTYFKDT---GDHVPKFILFLDHEIGSLVLSIRGTH-----SLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKA-EYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVLVRYDD 494
            I  +L   G +D+ E D    P  + L LI   Q    +AW N        D +      TF     +     +       ED  +Q +  M    ++DI+Y +  D    GDH P F L L  +   +VL+I GT       + DV +D+  D E FL GHAHRG+  G   +++++   +  TLEE+P Y +++TG+SLGAG ++L+TM   + ++ + IP  T ++C     PPV+ +D     +  +   +  NN D +    L ++ KL   ++A++++ M + D  +++    D
Sbjct:   55 IATDLDGDGLMDEMEEDDRWVPYLLTLGLIYKVQKNDSKAWYNHETSFLQNDDLQEKFLTTFGYFWHLLVEISKCIRTQSSEDVLEQLRTTMTCIPQEDIIYAHSTDQQHLGDHCPDFALTLQRDQKLVVLTICGTRMIPAPEMKDVFMDLYADTEPFLHGHAHRGMAIGXRNIVEKVLPFILSTLEENPGYEIMVTGYSLGAGISQLVTMEFSEGESRKVIPSGTIIRCFCYGTPPVFYSDEVQDYKCPNVYSVTFNN-DGLISASLTNVLKLFMQIKALERMHMRRRDMCRLVFSKSD 353          

BLAST of conserved unknown protein vs. L. salmonis genes
Match: EMLSAG00000004004 (supercontig:LSalAtl2s:LSalAtl2s214:147448:149600:-1 gene:EMLSAG00000004004 transcript:EMLSAT00000004004 description:"augustus_masked-LSalAtl2s214-processed-gene-1.4")

HSP 1 Score: 70.8626 bits (172), Expect = 4.672e-13
Identity = 52/178 (29.21%), Postives = 90/178 (50.56%), Query Frame = 0

Query:  318 FILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGH----AHRGIMRGSCKVLKQIQT--VLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVL 489
            F++ +D E   +V+SIRG+ ++ D I D++ D    +DG+     H G+M  +  + K++Q   +L + L  +P Y +++TGHSLGAG A +LT ++   +  Y        C A A P        +  E    I + +N  D  PR+   + A L+  +R V  L   +  ++K+L
Sbjct:  371 FMVIIDKEKEKIVISIRGSMTMNDAITDLLIDTTA-IDGYEQYYTHTGMMEAARNIYKELQDKDILNKALALNPGYGIVVTGHSLGAGXAVILTFLL---RILY----KSTICYAFACPVCLNERARLESERF-VINV-VNGDDVFPRLSPTNFALLVTQMRHV--LRYSRVPKYKIL 536          

BLAST of conserved unknown protein vs. SwissProt
Match: gi|114149273|sp|P0C1S9.1|DGLB_RAT (RecName: Full=Sn1-specific diacylglycerol lipase beta; Short=DGL-beta)

HSP 1 Score: 61.6178 bits (148), Expect = 1.150e-8
Identity = 43/127 (33.86%), Postives = 67/127 (52.76%), Query Frame = 0

Query:  280 YDA-SGEEDPKKQAQIMKIPS--EKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDE------EFLDGHAHRGIMRGSCKVLKQIQT--VLRQTLEEHPRYRLILTGHS 395
            YDA  G+E     A I+K      +D ++  F D    +P FI+ LDH   S+V+++RGT SL DV+ D+  + E      E  D  AH+GI + +  + +++    +L Q     P YRL++ GHS
Sbjct:  318 YDAVEGDEHHCHFASILKTTGLQYRDFIHVSFHDKVYELP-FIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESENLELDIELQDCVAHKGIAQAARYIYRRLVNDGILSQAFSVAPEYRLVVVGHS 443          

BLAST of conserved unknown protein vs. SwissProt
Match: gi|114149272|sp|Q8NCG7.2|DGLB_HUMAN (RecName: Full=Sn1-specific diacylglycerol lipase beta; Short=DGL-beta; AltName: Full=KCCR13L)

HSP 1 Score: 60.077 bits (144), Expect = 2.882e-8
Identity = 48/138 (34.78%), Postives = 76/138 (55.07%), Query Frame = 0

Query:  301 KDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDE------EFLDGHAHRGIMRGSCKVLKQI--QTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPP 430
            +D ++  F D    +P F++ LDH   S+V+++RGT SL DV+ D+  + E      E  D  AH+GI + +  V +++    +L Q     P YRL++ GHSLG G A LL  ++   +A Y     +V+C A +PP
Sbjct:  342 RDFIHVSFHDKVYELP-FLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDVECEVQDRLAHKGISQAARYVYQRLINDGILSQAFSIAPEYRLVIVGHSLGGGAAALLATML---RAAY----PQVRCYAFSPP 471          

BLAST of conserved unknown protein vs. SwissProt
Match: gi|81902338|sp|Q91WC9.2|DGLB_MOUSE (RecName: Full=Sn1-specific diacylglycerol lipase beta; Short=DGL-beta)

HSP 1 Score: 59.3066 bits (142), Expect = 5.939e-8
Identity = 85/346 (24.57%), Postives = 159/346 (45.95%), Query Frame = 0

Query:  280 YDA-SGEEDPKKQAQIMKIPS--EKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDE------EFLDGHAHRGIMRGSCKVLKQIQT--VLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPP--VYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKL-LAMVRAVDKLEMEKTDQFKVLVR---YD-------------DDETANNLKR--IQDEIMRIKQD--------------RFPFLEHPGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFD 579
            YDA  G++     A I+K      +D ++  F D    +P FI+ LDH   S+V+++RGT SL DV+ D+  + E      E  D  AH+GI + +  + +++    +L Q     P Y+L+L GHSLGAG A LL +++   +  Y     +V+  A +PP   + ++ +E  K+   ++   I   D +PR+ + ++  L   ++R +      K   +K+L+    Y              D+ T   L +  + ++ +  +                ++P L  PG+I +L +   SG+     + +        A F++ + +  +M++DH+    I+A D
Sbjct:  318 YDAVEGDQHNCHFASILKTTGLQYRDFIHISFHDKVYELP-FIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESETLELGIELQDCVAHKGIAQAARYIHRRLVNDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIML---RGAY----PQVRAYAFSPPRGLLSKSLYEYSKDFVVSL---ILGMDVIPRLSVTNMEDLKRRILRVIANCNKPK---YKILLHGCWYGLFGGSPDNFPTELDEGTQGALTQPLLGEQTLLTRYSPGYCSSDSPLDSPTKYPTLYPPGRIIHLEEEGGSGRFGCCSAAQYRARWAHEAEFSK-ILIGPKMLIDHMPDVMIRALD 648          

BLAST of conserved unknown protein vs. nr
Match: gi|299473078|emb|CBN77471.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 114.775 bits (286), Expect = 5.040e-23
Identity = 76/245 (31.02%), Postives = 116/245 (47.35%), Query Frame = 0

Query:  320 LFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEY--------------------IPEDTEVQCIAIAPPPVYRTDHEV---------------PKEMADAIRIYI---NNQDCVPRMCLGSIAKLLAMVRAVDKLEME--KTDQFKV--LVRYDDDETANNLKRIQDEIMRIKQDRFPFLEHP 522
            + +DH   S+V+SIRGT+S TD ++D++C+  +F  G AH+GI + + +V   ++  + + L EH  Y+L+LTGHSLGAGTA LL +++ +N  E                     +     V+C A APPPV+                     P   A A R +    +  DCVP M LGS+  L   ++ VDKL ++  K  +F +   VR    E        +D+  +I  D      HP
Sbjct:  203 VAVDHASRSVVISIRGTYSFTDTMVDLLCNTVDFAGGKAHQGISQSAVRVWTAVRGEVEKQLREHSDYKLVLTGHSLGAGTAILLKILLERNAMEALKGGFRLAKDKTAAKTARLDVGRPVRVECYAFAPPPVFSVPGATWMRDVYSFVNGLMQHPLSRAIARRWFWRRHDRADCVPTMSLGSLYSLCRTIQHVDKLPLDVYKRAEFVLDSRVRVQQSEQGLGAAGNEDDRRKIASDILGLRAHP 447          

BLAST of conserved unknown protein vs. nr
Match: gi|1325284735|ref|XP_023327070.1| (sn1-specific diacylglycerol lipase beta-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 110.153 bits (274), Expect = 6.343e-23
Identity = 71/224 (31.70%), Postives = 121/224 (54.02%), Query Frame = 0

Query:  274 SHALQVYDASGEEDPKKQAQIMKIPSEKDIVYTYFKDTG---DHVPKFILFLDHEIGSLVLSIRGTH-----SLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVL 489
            S  L  ++ S EE   K  + +   +E ++V  + KD     D  P   L + H+   +VL+I GT      S+ D+++D+ CD  E+L+G AH GI   +  VLK  Q  L  T   +P Y LI+ G+SLGAG + L+++ +L+N    +P   ++ CIA   PPVYR+ + +     +   + ++N D +P + L ++ KL   +RA+DKL++++     +L
Sbjct:   95 SWVLATHEKSVEEHQTKILESVN-SNEIEMVLFHVKDATTLEDQAPDTALIIHHDRQEIVLTICGTKITPAPSMGDIMLDLHCDSIEYLEGKAHEGIAIAAENVLKIFQEDLINTARMYPGYSLIIIGYSLGAGVSHLVSINLLQNN--LLPSSVKLYCIAYGAPPVYRSSN-ISYSHPNIFNV-LHNTDGLPTLSLHNVTKLFLQIRAIDKLKLKRRKILSLL 313          

BLAST of conserved unknown protein vs. nr
Match: gi|1072236305|gb|OEU21033.1| (alpha/beta-hydrolase [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 112.849 bits (281), Expect = 1.382e-22
Identity = 80/270 (29.63%), Postives = 137/270 (50.74%), Query Frame = 0

Query:  317 KFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKL---EMEKTDQFKVLVRYDD--DETANNLKRIQDEIMRIKQDRFPFLEHPGKIN-YLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDN 580
            + ++ +DHE   +VLSIRGT SL ++++DV     EF  G AH  +   + +V       +R  LE +P Y  ILTGHSLGAG A L+T ++++NK   +P++ +++C A A PPVY     VPK +  A   +++  D V  + +  + +L + +RAV+      M   +++KV++   +   E   ++   + +++  K+   P L  P K   +L K K +  GD +       V+       + + +   M  DH    Y  AFD+
Sbjct:  295 RHMVVVDHEHRKVVLSIRGTFSLEEIVLDVAAVSREFCGGEAHSEMANMAERVWTVAGPTVRNVLEMNPGYEFILTGHSLGAGAATLVT-ILVQNK-NLLPKEQKIRCFAYASPPVYTPLEFVPKSV-QATTNFVHQNDVVSFLSIQKVRQLFSSLRAVELYAHNHMSSRERYKVILGSSEPPKELIASVLEAEGKLLSPKKGA-PVLYIPAKNTIWLKKDKENNNGDYRFE-----VSNAREMTQKVIRVYPDMFTDHFPSRYEHAFDH 555          

BLAST of conserved unknown protein vs. nr
Match: gi|1129170292|gb|OLP80606.1| (Sn1-specific diacylglycerol lipase alpha [Symbiodinium microadriaticum])

HSP 1 Score: 109.383 bits (272), Expect = 4.122e-21
Identity = 84/271 (31.00%), Postives = 137/271 (50.55%), Query Frame = 0

Query:  328 SLVLSIRGTHSLTDVIIDVICDDEEFLDGH---AHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVLVRYD----DDETANNLKRIQDEIMRIKQDR-------FPFLEHPGKINYLYKLKPSGQ---GDQKLSEKMLVVTQESASFTRELFLL--ERMILDHLKPYYIQAFD 579
            ++VL IRGT +L D + D + D  +  +     AH+ ++  +  VL++ ++ L + L+E+P Y L++TGHSLGAGTA L T+++  N  +  P    ++C A APPPV    +  P   A  I  +IN  D VPR  L ++  L     AVD LE++   +F +L+R D    +D     L+++   +   +Q R       FP L  PG++ ++  L  SG      +  +E+   +   SA+  + L L      + DHL   Y +A D
Sbjct:  596 AVVLGIRGTTTLCDALTDAVGDAAKVPECSGLLAHKAMLASARAVLERTRSALNEALKENPGYSLVITGHSLGAGTAILCTILLNVNPLDSRP---AMKCFAFAPPPVVGPLNN-PSLRALEIHSFINRADIVPRASLANVFHLGQECMAVDGLELDFLHRF-ILMRRDPNPENDAEKQALQKVMGTVHDCRQLRKQKDHESFPPLFVPGQVYWIEWLGNSGSVQDTPEATAERTPRIHMASAAEFQSLLLRGGTNALKDHLCGNYKEALD 861          

BLAST of conserved unknown protein vs. nr
Match: gi|1210517977|dbj|GAX23143.1| (hypothetical protein FisN_33Lh035 [Fistulifera solaris])

HSP 1 Score: 107.457 bits (267), Expect = 8.170e-21
Identity = 66/199 (33.17%), Postives = 106/199 (53.27%), Query Frame = 0

Query:  297 IPSEKDIVYTYFKDT-GDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVLVRYDD 494
            +P E  +      DT G H+  F+  +DH    +VL+IRGT S +++++DV      F  G AH  +   + +V +  +  + + L  +  Y L+LTGHSLGAGTA LL ++  + + + + E   V+C A A PPV+     VPK   ++   YI ++D VP + + S+  LLA + AV     +   +F+VL R  D
Sbjct:  275 VPEEDIVSLDLDHDTDGYHLRHFVA-VDHINKQVVLAIRGTFSFSEIVVDVAAQSRPFCGGEAHAEMANMAERVWEAARETVMKVLRSNENYELVLTGHSLGAGTACLLHILCHQEERKLV-EGRNVRCFAYATPPVFSPLEFVPKSALNSCTGYIYDRDVVPFLSVDSVRHLLASLSAVGNQRPKLWQKFRVLARQSD 471          

BLAST of conserved unknown protein vs. nr
Match: gi|1013937236|gb|KYQ90932.1| (hypothetical protein DLAC_07808 [Tieghemostelium lacteum])

HSP 1 Score: 107.071 bits (266), Expect = 1.667e-20
Identity = 78/286 (27.27%), Postives = 136/286 (47.55%), Query Frame = 0

Query:  316 PKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPR-------YRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQ-----FKVLVRYD------DDETANNLKRIQDEIMRIKQDRFP--FLEHPGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDNC 581
            P   +  DH+  ++VL+IRGT    D++ D++  + +F DG+AH GI+R +     ++  VL   LE + R       Y +I+ GHSLG G A L T ++ K     IP    + C A APP +   +  +  E    I  ++ N D VPR+C  S+  LL +V  +    ++K D      F++L   +       D   + LK  +D +++ ++       +  PG++  +YK  P        +E   V+ + + SF +E+ +   ++ DH+   Y   F +C
Sbjct:  319 PGHYIAYDHKNKAIVLAIRGTFHARDILTDLVAKEIKFYDGYAHAGILRCAENKFAELAPVL---LEHYNRMDKDGKNYGIIVCGHSLGGGVASLFT-IMFKTNYPGIP----IHCYAYAPPSIGSMEISLSVEYRSLIDTFVFNDDIVPRLCYASLEHLLELVLCI----LKKNDSITQLGFQILAAGNTFGDQVTDRIGSLLKLKKDTMLKYQELHLSNKTMLPPGRMFRIYKNSP--------NEPHYVMEESNPSFFKEIIISNTLLTDHMPDKYELGFQSC 584          

BLAST of conserved unknown protein vs. nr
Match: gi|698476651|ref|XP_009785615.1| (PREDICTED: uncharacterized protein LOC104233865 [Nicotiana sylvestris] >gi|698476653|ref|XP_009785616.1| PREDICTED: uncharacterized protein LOC104233865 [Nicotiana sylvestris] >gi|698476656|ref|XP_009785617.1| PREDICTED: uncharacterized protein LOC104233865 [Nicotiana sylvestris] >gi|1025174553|ref|XP_016471448.1| PREDICTED: uncharacterized protein LOC107793583 [Nicotiana tabacum] >gi|1025174558|ref|XP_016471449.1| PREDICTED: uncharacterized protein LOC107793583 [Nicotiana tabacum])

HSP 1 Score: 104.375 bits (259), Expect = 3.361e-20
Identity = 67/194 (34.54%), Postives = 101/194 (52.06%), Query Frame = 0

Query:  271 RVSSHALQVYDASGEEDPKKQAQIMKIPSEKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRG----THSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTE-VQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPR 459
            R+  + L VY+    +   +  +   I  +  IV   + DTG  VP ++L+LDHE   +VL+IRG     HS   V++D      +F  G+ H G+++ +  VL     +LRQ LE+HP Y L  TGHSLG+G A LLT+V+ +N+      D + ++C AIAP      +  V    AD I   +   D +PR
Sbjct:   51 RLCRYILAVYEDDLRQPQWEPPEGYGINPDCLIVKKNYPDTGGRVPPYLLYLDHEHADIVLAIRGLDLAKHSDYAVLLDNKLGQRKFDGGYVHNGLLKAAGLVLNTECEILRQLLEKHPNYTLTFTGHSLGSGVAALLTLVVAQNRDRLGNIDRKRIRCFAIAPARCMSLNLAV--RYADVINSIVLQDDFLPR 242          

BLAST of conserved unknown protein vs. nr
Match: gi|831771317|ref|XP_012753266.1| (hypothetical protein SAMD00019534_067000 [Acytostelium subglobosum LB1] >gi|735853898|dbj|GAM23525.1| hypothetical protein SAMD00019534_067000 [Acytostelium subglobosum LB1])

HSP 1 Score: 104.76 bits (260), Expect = 6.079e-20
Identity = 75/298 (25.17%), Postives = 136/298 (45.64%), Query Frame = 0

Query:  297 IPSEKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRA-VDKLEMEKTDQFKVLVRYDDDETANNLKRIQDEIMRIKQD-RFPFLEH----------PGKINYLYKLKPSGQGDQKLSEKMLVVTQESASFTRELFLLERMILDHLKPYYIQAFDNCY 582
            I  E  I Y +     D  P   + +DH   ++V+S RGT    D++ D++  +  FLDG AH GI++ +     ++  +L + L++H  Y L++TGHSLGAGTA L T++      E      ++ C A APP V   +  + ++    I  Y+   D +PR+   S+  L  ++ + +D         F++L     +   +NL       +++++  + P  ++          PG    +YK        Q   +K  ++ + + S   E+ +   ++LDH   +Y  A   CY
Sbjct:  180 IQREDVICYRFTSHNFD--PAHFVAVDHATMAIVVSFRGTFHARDILTDLVATNTPFLDGFAHTGILKCAQNKFNELTPLLLENLKKHAGYSLVVTGHSLGAGTAALFTLLFHSTYPE-----IKIHCHAYAPPCVTSLELALDEKTRSLITSYVLKDDIIPRLSYQSLEHLKHLITSMLDNNPGMINQAFQILCA--GNNLGDNLTNKVASFLKVERGVKLPSKQYAALNTQTMLPPGDTYRIYK--------QSNFDKHFIMEKSNPSLFGEIVISGSLLLDHFPDHYEDALSLCY 460          

BLAST of conserved unknown protein vs. nr
Match: gi|1111035221|ref|XP_019224440.1| (PREDICTED: uncharacterized protein LOC109206121 [Nicotiana attenuata] >gi|1102286559|gb|OIT33349.1| hypothetical protein A4A49_15007 [Nicotiana attenuata])

HSP 1 Score: 103.605 bits (257), Expect = 6.747e-20
Identity = 66/194 (34.02%), Postives = 101/194 (52.06%), Query Frame = 0

Query:  271 RVSSHALQVYDASGEEDPKKQAQIMKIPSEKDIVYTYFKDTGDHVPKFILFLDHEIGSLVLSIRG----THSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTE-VQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPR 459
            R+  + L VY+    +   +  +   I  +  IV   + DTG  +P ++L+LDHE   +VL+IRG     HS   V++D      +F  G+ H G+++ +  VL     +LRQ LE+HP Y L  TGHSLG+G A LLT+V+ +N+      D + ++C AIAP      +  V    AD I   +   D +PR
Sbjct:   51 RLCRYILAVYEDDLRQPQWEPPEGYGINPDCLIVKKNYSDTGGRLPPYLLYLDHEHADIVLAIRGLDLAKHSDYAVLLDNKLGKRKFDGGYVHNGLLKAAGLVLNTECEILRQLLEKHPNYTLTFTGHSLGSGVAALLTLVVAQNRDRLGNIDRKRIRCFAIAPARCMSLNLAV--RYADVINSIVLQDDFLPR 242          

BLAST of conserved unknown protein vs. nr
Match: gi|1210512248|dbj|GAX28689.1| (hypothetical protein FisN_33Hh035 [Fistulifera solaris])

HSP 1 Score: 104.76 bits (260), Expect = 7.600e-20
Identity = 65/199 (32.66%), Postives = 104/199 (52.26%), Query Frame = 0

Query:  297 IPSEKDIVYTYFKDT-GDHVPKFILFLDHEIGSLVLSIRGTHSLTDVIIDVICDDEEFLDGHAHRGIMRGSCKVLKQIQTVLRQTLEEHPRYRLILTGHSLGAGTAELLTMVILKNKAEYIPEDTEVQCIAIAPPPVYRTDHEVPKEMADAIRIYINNQDCVPRMCLGSIAKLLAMVRAVDKLEMEKTDQFKVLVRYDD 494
            +P E  +      DT G H+  F+  +DH    +VL+IRGT S +++++DV      F  G AH  +   + +V +  +  + + L  +  Y L+LTGHSLGAGTA LL ++  +++ + + E   V+C A A PPV+     VPK   ++   YI ++D VP + + S+  LLA + AV         + KV  R  D
Sbjct:  297 VPEEDIVSLDLDHDTEGYHLRHFVA-VDHINQQVVLAIRGTFSFSEIVVDVAAQSRPFCGGEAHAEMANMAERVWEAARETVMKVLRSNENYELVLTGHSLGAGTACLLHILCHQDERKLV-EGRNVRCFAYATPPVFSPLEFVPKSALNSCTAYIYDRDVVPFLSVDSVRHLLASLSAVGNQRPRLWQKVKVFARRSD 493