BLAST of alkaline phosphatase 4 vs. L. salmonis genes
Match: EMLSAG00000001931 (supercontig:LSalAtl2s:LSalAtl2s1338:61614:66677:-1 gene:EMLSAG00000001931 transcript:EMLSAT00000001931 description:"maker-LSalAtl2s1338-augustus-gene-0.10")

HSP 1 Score: 277.33 bits (708), Expect = 5.135e-88
Identity = 167/482 (34.65%), Postives = 247/482 (51.24%), Query Frame = 0

Query:   61 ILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQE-VSTILSWAQEGGKDTE-------------------------------------GLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPN--------SAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPED--PTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDE-TLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLG 493
            +   L     NT+KA+N+I+F+GDGM +PT++A RIFK Q  G+    E+ YL+F++FPH+G SK Y+ D Q PDSA+ ASA++SG+KT +Y +G D+S     + S +     V+ I++W+Q   K T                                      G  DI +Q+ ++ PG   +VILGGG   + PK K           YD  +NC+R D L+L + W  N  N           V  +  LL +D   TDY+LGL   SH+ +  A    +  P++ +M   A+ IL+KN E GFFL++EG +ID  HH+  A RAL ET A D   +AV+ AL    +E TLI+VTA   H +S   Y  RG D               P ++YAN   +  H   D    + R+ +   +  D +F +P+       +H   DV I+A GPW+H  H+ HEQ+++AHVM++ +C+G
Sbjct:   10 VAEELYRKRINTEKARNIILFIGDGMGLPTISATRIFKAQSQGSTRGGEESYLSFDKFPHIGLSKVYNVDAQSPDSAAXASALYSGIKTNYYCVGLDNSCKKGSLESHVNESAWVTNIMTWSQLANKSTGFVTTSRVSHATPSSLVAHSALRKWECDKAMPDGASEIGAKDITFQMAKRSPGKKARVILGGGRTTWRPKQKD--------VNYD-GFNCWRTDGLNLIESWM-NKSNVLGMENMKGRYVTTKDELLELDYENTDYVLGLFSESHMEYVSAEKDSNSQPSISEMTESALKILQKNPE-GFFLMVEGAKIDKGHHEGKANRALMETSAFD---DAVLTALNMTSEEDTLIIVTAXXGHXISFGAYEQRGVD-------------SLPILAYANSPGFNKHYDVDEEKQIIRKEMKDLKYTDPDFLNPAGVQNWESTHSAADVPIYARGPWAHFLHSVHEQSYVAHVMSYGACIG 464          

BLAST of alkaline phosphatase 4 vs. L. salmonis genes
Match: EMLSAG00000006434 (supercontig:LSalAtl2s:LSalAtl2s351:555455:577638:-1 gene:EMLSAG00000006434 transcript:EMLSAT00000006434 description:"maker-LSalAtl2s351-augustus-gene-5.17")

HSP 1 Score: 255.758 bits (652), Expect = 2.055e-75
Identity = 175/516 (33.91%), Postives = 258/516 (50.00%), Query Frame = 0

Query:   29 HHGPIARHASSSNRDVAAQEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDV-TSMLTAQEVSTILSWAQEGGKDT-------------------------------------EGLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPN--SAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEG----FFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGL---------STEVYDDDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSC 491
            + GP  +   S ++     E+  YW   G  +I   L+++  N  K KNVI+ +GDGM + ++T  RI+KGQ  G +   E+E L++E FPH G SKTY+TDYQ+PDSA TA+A+ SG KTK   LG D+S++++      L A ++  I + A +  K+T                                     EG  D+A Q++   PG  + V+ GGG   F  KD      ++E                DL   W+    N  S  +  +S L   + A T+Y+L       V+ D  +   DP+  D++L A++ L+   +EG    F LL+E GRID AHH   A++A  E V MD+ V  +M  +   L +TL++VTADHSH +++NGY  RGN I G           T+   + +PY TISYANG  +  H I   N N+        E +D + ++        ESHGG+DV I+A GP SHLF   HEQ++I+HV+ +  C
Sbjct:  533 YSGPTPQ---SQSKHTQKHENQNYWRSQGIEDIKFKLSQSQNN-GKVKNVILIIGDGMGLNSITPGRIYKGQKAGKL--GEEETLSWEYFPHTGLSKTYNTDYQVPDSAGTATAILSGXKTKMAFLGIDNSVEYNTCDPKKLEAAKLENIFTMAIKNKKETGIVTTTRITHATPGAAYAATSNRDWEADSDMSKEFISEGCTDVAKQMIYSSPGNQINVLFGGGRSKFMKKDAGGK-RLDE----------------DLIYVWKNFKKNVSSKYLDSKSDLDQWNIANTEYVL-------VSRDVEK---DPSFTDLSLAALNRLQTAYDEGKSNGFVLLLESGRIDMAHHTNHAKKAFEELVEMDRAVNLIMDKV--NLQDTLVIVTADHSHAVTMNGYPHRGNSILGYIFEDRGGNYVTKENGEKIPYSTISYANGPGFKKHFI---NNNMVDIRKLDYESDDYQSQAMWLGPDNYESHGGEDVPIYAIGPQSHLFTGVHEQSYISHVIHYGMC 1010          

HSP 2 Score: 246.128 bits (627), Expect = 6.135e-72
Identity = 167/507 (32.94%), Postives = 255/507 (50.30%), Query Frame = 0

Query:   41 NRDVAAQEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVT-SMLTAQEVSTILSWAQEGGKDT-------------------------------------EGLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPN--SAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEG----FFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDD------------LPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSC 491
            N+     ED  YW  LG  +I   L+++  N  + KNVI+ +GDGM I T+T  RI+KGQ  G +   E+E L++E FP+ G SKTY+T+YQ+PDSA TA+A+ SG KT+   LG D+S++        L A ++  I + A +  K+T                                     +G  D+A Q++   PG  + V+ GGG   F  K+      ++E                DL   W+    N  S  +  +S L   +   T+Y+L       V+ D  +   DP+  D++L A++ L+   +EG    F LL+E GRID AHH  + ++A  E V MD+ V  +M  +   L++TL++VTADHSH +S+NGY +RGN+I G    VYD+             +P+ TISYANG  +  H     N ++        E +D + ++   A  +  +HGG+DV I+A GP S+LF   HEQ++I+HV+ +  C
Sbjct:    3 NKPTPRIEDQNYWRSLGIEDIKFKLSQSQNN-GEVKNVILIIGDGMGINTITPGRIYKGQKAGKL--GEEETLSWEYFPYTGLSKTYNTNYQVPDSAGTATAILSGCKTRMSVLGIDNSVEXGTCXPKKLEAAKLENIFTMAIKNKKETGIVTTTRITHATPGAAYAATSNRDWEADSDMSKEFISKGCTDVAKQMIYSSPGNQINVLFGGGRSNFL-KESAGGKRLDE----------------DLISVWKNFKKNVSSKYLDSKSDLDQWNIDNTEYVL-------VSRDVEK---DPSFTDLSLAALNRLQTAYDEGKSNGFVLLLESGRIDTAHHTSYPKKAFEELVEMDRAVNLMMKRI--NLNDTLVIVTADHSHGISMNGYPSRGNNILGY---VYDEKFGNHMTRPNGEKVPFSTISYANGPGFSKHF---ANNDIVDIRSLDYESDDYQTQTMWLAPDEXATHGGEDVPIYAIGPQSYLFTGVHEQSYISHVINYGMC 471          

BLAST of alkaline phosphatase 4 vs. L. salmonis genes
Match: EMLSAG00000005087 (supercontig:LSalAtl2s:LSalAtl2s2693:1242:7030:-1 gene:EMLSAG00000005087 transcript:EMLSAT00000005087 description:"maker-LSalAtl2s2693-augustus-gene-0.4")

HSP 1 Score: 150.599 bits (379), Expect = 2.399e-41
Identity = 111/331 (33.53%), Postives = 165/331 (49.85%), Query Frame = 0

Query:   48 EDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQ----INGNMDS-AEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQEVSTILSWAQEGGKDT----------------------EG-LHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPA----------TTDYLLGLLGRSHVAFD---DARGPE-DPTLEDMALKAIDILKKN-SEEGFFLLIEGGRIDHAHH 335
            ED  YW ++   E+ R+L   T N  KAKNVI+F+GDGMS+PTLTA RIFK Q      GN     E+  LTFE FP++G S+      QI        A+ +GVKT + ++G+D+S+      S + A +V +IL WAQ+ G DT                      EG  +DIA Q    +PG  +K+++GGG  A+      + +    +  ++    C R D+ +L +++ + +   +   ++      D A          + DYLLGL   S +  D   + R  E +P + +M   A+  L KN S  GFFL+IEGG ID +HH
Sbjct:   25 EDAAYWRKVNDKELQRALRH-TPNKNKAKNVILFIGDGMSLPTLTAGRIFKNQRLAEKRGNEGPVGEETPLTFETFPNVGLSRCSLKMLQI-----YRLAIATGVKTXYGTMGFDNSVKRGSGESQVKATKVESILKWAQDVGMDTGAFYANTAERNWECDSAIPVEQEGDFYDIAKQAXYNDPGRRLKLLMGGGRKAWVNNPSPSKMSKRPENEFE----CSRKDNENLIQKFLKKNEEVSGFENQKGRYIKDYAELSKAMNEMNSYDYLLGLFSDSLMQLDSEINKRSLEREPDIVEMTEFAVKFLSKNESSNGFFLMIEGGMIDKSHH 345          

BLAST of alkaline phosphatase 4 vs. L. salmonis genes
Match: EMLSAG00000001932 (supercontig:LSalAtl2s:LSalAtl2s1338:70062:70743:-1 gene:EMLSAG00000001932 transcript:EMLSAT00000001932 description:"maker-LSalAtl2s1338-snap-gene-0.11")

HSP 1 Score: 68.5514 bits (166), Expect = 5.502e-14
Identity = 31/96 (32.29%), Postives = 51/96 (53.12%), Query Frame = 0

Query:  406 PTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFA--------TGPWSHLFHTTHEQTHIAHVMAFSSCLG 493
            P + YAN   +  H   D    + R+ +   +  D +F +P+A      +H   DV I+A         GPW+H FH+ HEQ+++AHVM++ +C+G
Sbjct:   32 PILVYANSPGFNKHYDVDEEKQIIRKEMKDLKYTDPDFLNPAAVQSWESTHSAADVPIYARGKYQYFFLGPWAHFFHSVHEQSYVAHVMSYGACIG 127          

BLAST of alkaline phosphatase 4 vs. SwissProt
Match: gi|341941723|sp|P09242.2|PPBT_MOUSE (RecName: Full=Alkaline phosphatase, tissue-nonspecific isozyme; Short=AP-TNAP; Short=TNSALP; AltName: Full=Alkaline phosphatase 2; AltName: Full=Alkaline phosphatase liver/bone/kidney isozyme; Flags: Precursor)

HSP 1 Score: 296.975 bits (759), Expect = 1.691e-93
Identity = 190/497 (38.23%), Postives = 266/497 (53.52%), Query Frame = 0

Query:   47 QEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQEVSTILSWAQEGGKD-------------------------------------TEGLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEE---NHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGP-EDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYD-DDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            + D  YW Q  Q  +  +L     NT  AKNVI+F+GDGM + T+TAARI KGQ++ N  + E+  L  ++FP +  SKTY+T+ Q+PDSA TA+A   GVK    ++G  ++ +     +     EV++IL WA++ GK                                      ++G  DIAYQL+       + VI+GGG    +PK++   +E E     DE     R D LDL   W+     H +S  V +R+ LLA+DP+  DYLLGL     + ++  R    DP+L +M   A+ IL KN + GFFLL+EGGRIDH HH+  A++AL E V MD+ +     A+  Q D TL VVTADHSH  +  GY  RGN I GL+  V D D  P+  I Y NG  Y    + DG     R  ++  +     +++ SA   + E+HGG+DVA+FA GP +HL H  HEQ +I HVMA++SC+G   N  HC
Sbjct:   23 ERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHN--TGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTT-QGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIK--DIDVIMGGGRKYMYPKNRTD-VEYE----LDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLK-GFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKA-GAMTSQKD-TLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGY---KVVDGE----RENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIG--ANLDHC 497          

BLAST of alkaline phosphatase 4 vs. SwissProt
Match: gi|146345490|sp|P09487.2|PPBT_BOVIN (RecName: Full=Alkaline phosphatase, tissue-nonspecific isozyme; Short=AP-TNAP; Short=TNSALP; AltName: Full=Alkaline phosphatase liver/bone/kidney isozyme; Flags: Precursor)

HSP 1 Score: 295.819 bits (756), Expect = 4.110e-93
Identity = 186/497 (37.42%), Postives = 266/497 (53.52%), Query Frame = 0

Query:   47 QEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQEVSTILSWAQEGGKD-------------------------------------TEGLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEE---NHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARG-PEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYD-DDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            ++D +YW    Q  +  +L   T NT  AKNVI+F+GDGM + T+TAARI KGQ++      E+  L  ++FP++  SKTY+T+ Q+PDSA TA+A   GVK    ++G  ++       +     EV++IL WA++ GK                                      ++G  DIAYQL+       ++VI+GGG    FPK++   +E E     DE     R D L+L   W+     H +S  V +R+ LLA+DP + DYLLGL     + ++  R    DP+L +M   AI IL KN + GFFLL+EGGRIDH HH+  A++AL E V MD+ +    A     +++TL VVTADHSH  +  GY  RGN I GL+  V D D  P+  I Y NG  Y    +  G     R  ++  +     +++ SA   + E+HGG+DVA+FA GP +HL H  HEQ +I HVMA+++C+G   N  HC
Sbjct:   23 EKDPKYWRDQAQQTLKNALRLQTLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLH--HSPGEETKLEMDKFPYVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATQRSQCNTT-QGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSASYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIK--DIEVIMGGGRKYMFPKNRTD-VEYE----LDEKARGTRLDGLNLIDIWKSFKPKHKHSHYVWNRTDLLALDPHSVDYLLGLFEPGDMQYELNRNNATDPSLSEMVEMAIRILNKNPK-GFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIG--QAGAMTSVEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGY---KVVGGE----RENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYAACIG--ANRDHC 497          

BLAST of alkaline phosphatase 4 vs. SwissProt
Match: gi|130751|sp|P08289.2|PPBT_RAT (RecName: Full=Alkaline phosphatase, tissue-nonspecific isozyme; Short=AP-TNAP; Short=TNSALP; AltName: Full=Alkaline phosphatase liver/bone/kidney isozyme; Flags: Precursor)

HSP 1 Score: 295.434 bits (755), Expect = 4.982e-93
Identity = 188/497 (37.83%), Postives = 266/497 (53.52%), Query Frame = 0

Query:   47 QEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQEVSTILSWAQEGGKD-------------------------------------TEGLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEE---NHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGP-EDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYD-DDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            ++D  YW Q  Q  +  +L     NT  AKN+I+F+GDGM + T+TAARI KGQ++ N  + E+  L  ++FP +  SKTY+T+ Q+PDSA TA+A   GVK    ++G  ++ +     +     EV++IL WA++ GK                                      ++G  DIAYQL+       + VI+GGG    +PK++   +E E     DE     R D LDL   W+     H +S  V +R+ LLA+DP+  DYLLGL     + ++  R    DP+L +M   A+ IL KN + GFFLL+EGGRIDH HH+  A++AL E V MD+ +      +  Q D TL VVTADHSH  +  GY  RGN I GL+  V D D  P+  I Y NG  Y    + DG     R  ++  +     +++ SA   + E+HGG+DVA+FA GP +HL H  HEQ +I HVMA++SC+G   N  HC
Sbjct:   23 EKDPSYWRQQAQETLKNALKLQKLNTNVAKNIIMFLGDGMGVSTVTAARILKGQLHHN--TGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTT-QGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIK--DIDVIMGGGRKYMYPKNRTD-VEYE----LDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNPK-GFFLLVEGGRIDHGHHEGKAKQALHEAVEMDEAI-GKAGTMTSQKD-TLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGY---KVVDGE----RENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIG--ANLDHC 497          

BLAST of alkaline phosphatase 4 vs. SwissProt
Match: gi|2499544|sp|Q29486.1|PPBT_FELCA (RecName: Full=Alkaline phosphatase, tissue-nonspecific isozyme; Short=AP-TNAP; Short=TNSALP; AltName: Full=Alkaline phosphatase liver/bone/kidney isozyme; Flags: Precursor)

HSP 1 Score: 291.197 bits (744), Expect = 2.194e-91
Identity = 183/498 (36.75%), Postives = 265/498 (53.21%), Query Frame = 0

Query:   47 QEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQEVSTILSWAQEGGKD-------------------------------------TEGLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAM-LEMEEKTGYDENWNCYRNDSLDLAKEWEE---NHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPE-DPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYD-DDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            ++D +YW    Q  +  +L     NT   KNVI+F+GDGM + T+TAARI KGQ++ N    E+  L  ++FP++  SKTY+T+ Q+PDSA TA+A   GVK    ++G  ++       +     EV++IL WA++ GK                                      ++G  DIAYQL+       ++VI+GGG    FPK++  +  EM+EK          R D L+L   W+     H +S  V +R+ LL +DP   DYLLGL     + ++  R    DP+L +M   AI IL KN + GFFLL+EGGRIDH HH+  A++AL E V MD+ +    A     +++TL +VTADHSH  +  GY  RGN I GL+  V D D  P+ +I Y NG  Y    +  G     R  ++  +     +++ SA   + E+HGG+DVA+FA GP +HL H  HEQ +I HVMA+++C+G   N  HC
Sbjct:   23 EKDPKYWRDQAQQTLKNALRLQKLNTNVVKNVIMFLGDGMGVSTVTAARILKGQLHHN--PGEETRLEMDKFPYVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATQRTQCNTT-QGNEVTSILRWAKDSGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNV--RDIEVIMGGGRKYMFPKNRTDVEYEMDEKA------RGTRLDGLNLVDIWKSFKPRHKHSHYVWNRTELLTLDPYGVDYLLGLFEPGDMQYELNRNSTTDPSLSEMVEIAIKILSKNPK-GFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGRAGAM--TSVEDTLTIVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTSILYGNGPGY---KVVGGE----RENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYAACIG--ANLDHC 497          

BLAST of alkaline phosphatase 4 vs. SwissProt
Match: gi|29427685|sp|Q24238.3|APH4_DROME (RecName: Full=Alkaline phosphatase 4; Flags: Precursor)

HSP 1 Score: 293.123 bits (749), Expect = 3.201e-91
Identity = 200/556 (35.97%), Postives = 290/556 (52.16%), Query Frame = 0

Query:   12 HLILILSYFCQLSVTFHHHG------PIARHASSSNRDVAAQ---------EDMEYWNQLGQGEILRSLTEAT-----TNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQE--VSTILSWAQEGGK-----------------------------DTE------GLH-DIAYQLVRQEPGMSMKVILGGGFPAF----FPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPN----SAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSE-EGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLS-TEVYDDDLPYPTISYANGVNYYYH----NIPDGNGNV--TRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLG 493
            H ++IL +     V F   G      P+ R  S+   D+  Q         ED E+W+ +G  ++ +++ +A      +  KKA+N+IIF+GDGM I T++A RI+KGQ   +    E+E L F+ FP+ G +KTY+ D Q+PDSA TA+A+FSG KT + ++G D++       S    Q+  V +++ WAQ+ GK                             DTE      G H DIA QLV   PG    VILGGG          + K  + E   +T       C R D+ +L  EW  +H N     A+V +R  LL V+    D+L+GL   +H+ +  AR   +P+L++M   A+ IL++  E  GF LL+EGGRID  HH  +AR AL E    D  ++A  A      DETLI+VTADHSH ++ NGYA RG DI G + +   +D + Y TISYANG  Y+ H    + P  + N+    +  T EE     +R  +   +K E+HGG+DVA+FA GP S L     EQ ++A+VM+++ CLG
Sbjct:    2 HCLVILGFLLGSLVAFSWAGVTTQPPPLIRTLSAGG-DIGPQFDVGKTKEPEDAEFWHNVGLRQLEKTIKQAQRVKEDSYQKKARNIIIFIGDGMGISTISAGRIYKGQYLKH-GYGEEETLVFDDFPNTGMAKTYNVDKQVPDSAGTATAIFSGSKTHYGAIGMDAT------RSKKNGQQGRVQSVMEWAQKEGKRTGVVTTTRITHATPAATYAHIYDRDWECDTEVPAESVGFHVDIARQLVENAPGNRFNVILGGGMSPMGILNASEVKTTIFEGPTET------ICTRGDNRNLPAEWLAHHANDTVPPALVHNRKDLLNVNVKKVDHLMGLFRNNHITYSIAREAGEPSLQEMTETALGILERGDESNGFVLLVEGGRIDQGHHMNYARAALHELYEFDLAIQA--AVNNTDPDETLILVTADHSHAVTFNGYALRGADILGTANSHEKNDPMFYETISYANGPGYWDHLANDSRPQNSSNMWMPLKHFTAEERAAPTYRHLATVPRKDETHGGEDVAVFAYGPGSSLIRGVFEQNYLAYVMSYAGCLG 541          

BLAST of alkaline phosphatase 4 vs. SwissProt
Match: gi|68067533|sp|P05186.4|PPBT_HUMAN (RecName: Full=Alkaline phosphatase, tissue-nonspecific isozyme; Short=AP-TNAP; Short=TNSALP; AltName: Full=Alkaline phosphatase liver/bone/kidney isozyme; Flags: Precursor)

HSP 1 Score: 290.426 bits (742), Expect = 4.348e-91
Identity = 181/497 (36.42%), Postives = 264/497 (53.12%), Query Frame = 0

Query:   47 QEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQEVSTILSWAQEGGKD-------------------------------------TEGLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHP---NSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGP-EDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYD-DDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            ++D +YW    Q  +  +L     NT  AKNVI+F+GDGM + T+TAARI KGQ++ N    E+  L  ++FP +  SKTY+T+ Q+PDSA TA+A   GVK    ++G  ++ +     +     EV++IL WA++ GK                                      ++G  DIAYQL+       + VI+GGG    +PK+K   +E E     DE     R D LDL   W+   P   +S  + +R+ LL +DP   DYLLGL     + ++  R    DP+L +M + AI IL+KN + GFFLL+EGGRIDH HH+  A++AL E V MD+ +    +    +  +TL VVTADHSH  +  GY  RGN I GL+  + D D  P+  I Y NG  Y    +  G     R  ++  +     +++ SA   + E+HGG+DVA+F+ GP +HL H  HEQ ++ HVMA+++C+G   N  HC
Sbjct:   23 EKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHN--PGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTT-QGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNI--RDIDVIMGGGRKYMYPKNKTD-VEYES----DEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPK-GFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSE--DTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGY---KVVGGE----RENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLHGVHEQNYVPHVMAYAACIG--ANLGHC 497          

BLAST of alkaline phosphatase 4 vs. SwissProt
Match: gi|41017428|sp|P83456.1|PPB_GADMO (RecName: Full=Alkaline phosphatase; Short=AP)

HSP 1 Score: 280.796 bits (717), Expect = 7.076e-88
Identity = 175/488 (35.86%), Postives = 259/488 (53.07%), Query Frame = 0

Query:   47 QEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQEVSTILSWAQEGGK-----------------------------DTE--------GLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSA--VVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPE-DPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYD-DDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLG 493
            + + ++WN   Q  + ++L+  T N  KA+N+I+F+GDGM +PT+TAARI KGQ+ G     E+  L  ++FP +  SKTY+T+ Q+ DSA TA+A   GVK    ++G  ++       +     EV++IL WA++ GK                             D E        G  DIA QL    P   + VI+GGG    +PK+   +    E  G  ++ +  R D  +L KEW + +       V ++  LL+++P   DYLLGL   + + +D  R  E DP+L +M   AI IL++N   GF+LL+EGGRIDH HH+   ++A+ E V MD+ +    A L     +TL VVTADHSH  S  GY  RGN+I GL+  + D D  P+  I Y NG  Y   N         R  ++  + +D  + + +A    SE+HGG+DVA+FA GP +HL H  HEQ +I H MA+++C+G
Sbjct:    7 EPEPKFWNDWAQKTLDKALSLQTLNKNKAQNLILFLGDGMGVPTVTAARILKGQLRGQ--PGEEGQLEMDKFPFVALSKTYNTNAQVADSAGTATAYLCGVKANEGTVGVSAAAVRSQANTT-QGNEVTSILRWAKDAGKSIGIVTTTRVNHATPSAAYAHCVDRDWYSDNEMPADAVEAGCKDIARQLFENIP--DIDVIMGGGRKYMYPKNTTDV----EYPGQPKH-SGTRKDGRNLVKEWVDRNTEKKGHYVWNKKDLLSLNPTKVDYLLGLFEPADLPYDLERNKETDPSLSEMVEVAIKILRRNPN-GFYLLVEGGRIDHGHHEGKDKQAIHEAVEMDRAIG--RADLMTSTSDTLTVVTADHSHLFSFGGYTPRGNEIFGLAAFISDVDQKPFTAILYGNGPGYKLVN-------GARENVSTVDYQDNSYLAQAAVPLSSETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHAMAYAACIG 474          

BLAST of alkaline phosphatase 4 vs. SwissProt
Match: gi|6093774|sp|Q92058.1|PPBT_CHICK (RecName: Full=Alkaline phosphatase, tissue-nonspecific isozyme; Short=AP-TNAP; Short=TNSALP; Flags: Precursor)

HSP 1 Score: 281.182 bits (718), Expect = 1.523e-87
Identity = 182/497 (36.62%), Postives = 259/497 (52.11%), Query Frame = 0

Query:   47 QEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQEVSTILSWAQEGGK-----------------------------DTE--------GLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVR---DRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPE-DPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYD-DDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            ++D EYW Q  Q  +  +L     N   AKN+I+F+GDGM + T+TAARI KGQ+       E+  L  ++FP++  +KTY+T+ Q+PDSA TA+A   GVK    ++G  + +   D  +    QEV++IL WA++ GK                             D E        G  DIA QLV   P   ++VILGGG    FPK+   +   +E     E     R D  DL + W +  P   V +    R  LLA++ +  D+LLGL     + ++  R  E DP+L +M   AI +L+KN   GFFLL+EGGRIDH HH+  A++AL E V +D+ V   +A       +TL VVTADHSH  +  GY  RGN I GL+    D D  P+ +I Y NG  Y    I  G     R  ++  +     +++ +A   + E+HGG+DVA+FA GP +HL H   EQ +I H MA+++C+G   N AHC
Sbjct:   22 EKDPEYWRQQAQETLRDALRLQHLNQNVAKNLILFLGDGMGVSTVTAARILKGQLQHR--KGEESLLEMDKFPYVALAKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAGVTR-DRCNTTKGQEVTSILRWAKDEGKAVGIVTTTRVTHATPSAAYAHSANRDWYSDGEMPLDALEGGCKDIARQLVDNIP--DIEVILGGGRKYMFPKNTSDVEYPQE-----ERHRGTRLDGKDLVQAWHDTKPAGKVAKYVWHRRELLALNVSRVDFLLGLFEPGDMVYELDRNNETDPSLSEMVAVAIRMLQKNPR-GFFLLVEGGRIDHGHHEGKAKQALHEAVELDRAVG--LAGRLTSPRDTLSVVTADHSHVFTFGGYTPRGNPIFGLAPMQSDVDRKPFTSILYGNGPGY---KIVGGE----RENVSAVDFAHANYQAQAAVPLRQETHGGEDVAVFARGPMAHLLHGVDEQNYIPHAMAYAACIG--PNRAHC 496          

BLAST of alkaline phosphatase 4 vs. SwissProt
Match: gi|544584820|sp|P29523.4|PPB_BOMMO (RecName: Full=Membrane-bound alkaline phosphatase; Short=M-ALP; Flags: Precursor)

HSP 1 Score: 278.1 bits (710), Expect = 4.484e-86
Identity = 184/514 (35.80%), Postives = 262/514 (50.97%), Query Frame = 0

Query:   27 FHHHGPIARHASSSNRDVAAQEDMEYWNQLGQGEILRSLTE---ATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDV-TSMLTAQEVSTILSWAQEGGKDT---------------------------------EG-----LHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAV----VRDRSSLLAVDPATTDYLLGLLGRSHVAFD-DARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLG 493
            +H     A  AS++ R  AA+ +  +W +  Q  I R   E   A      AKNV++F+GDGMS+PTL AAR   GQ  G   + E+  L FE+FP LG +KTY  + Q+PDS+ TA+A   GVK    +LG  +++  HD   S    + V +I  WA   G+D                                  EG       DIA+QL++  PG   KVI GGG   F P  +   ++ E   G        R D  +L +EW+ +  +  V    + +R  LL +  +  DYLLGL   SH+ +  +     +PTL ++   AI +L +N E GFFL +EGGRIDHAHHD +A  AL ET+ MD+ V+    AL +  DE+L+VVTADH+H MS NGY+ RG D+ G    +  + +P+  +SY NG           NG V   V T      + +R+ +     SE+HGGDDV +FA G    +F   +EQTH+ H MA+++C+G
Sbjct:   24 YHPERLAAGEASAATRS-AAESEASFWVREAQEAIERREREGAGAKQAAGHAKNVVMFLGDGMSVPTLAAARTLLGQRRGQ--TGEEASLHFEQFPTLGLAKTYCVNAQVPDSSCTATAYLCGVKANQGTLGVTAAVPRHDCEASTDVTKRVQSIAEWALADGRDVGIVTTTRITHASPAGTFAKVANRNWENDNDVKQEGHDVNRCPDIAHQLIKMAPGNKFKVIFGGGRREFLPTTQ---VDEEGTRGL-------RTDGRNLIEEWQNDKESQKVSYKYLWNRQELLKLGSSPPDYLLGLFEGSHLQYHLEGDESTEPTLAELTDVAIRVLSRN-ERGFFLFVEGGRIDHAHHDNYAHLALDETIEMDRAVKVATDALKE--DESLVVVTADHTHVMSFNGYSPRGTDVLGTVRSLDSNRMPFMVLSYTNGPGARIQQ----NG-VRPDVTTDANFGALRWRTHTDVPLDSETHGGDDVTVFAWGVHHWMFSGLYEQTHVPHRMAWAACMG 516          

BLAST of alkaline phosphatase 4 vs. SwissProt
Match: gi|130745|sp|P24822.1|PPBI_MOUSE (RecName: Full=Intestinal-type alkaline phosphatase; Short=IAP; Short=Intestinal alkaline phosphatase; AltName: Full=Alkaline phosphatase 3; Flags: Precursor)

HSP 1 Score: 274.633 bits (701), Expect = 1.244e-84
Identity = 181/491 (36.86%), Postives = 245/491 (49.90%), Query Frame = 0

Query:   47 QEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTAQEVSTILSWAQEGGKDT-------------------------------------EGLHDIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLL--AVDPATTDYLLGLLGRSHVAFDDARGP-EDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPDGNGNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTN 497
            +E+  +WN+    E L +  +       AKN+IIF+GDGM +PT+TA RI KGQ+ G++    +  L  +RFP++  SKTY  D Q+PDSASTA+A   GVKT + ++G  S+    D  +     EV +++  A++ GK                                       EG  DIA QL+     M + VILGGG    FP         + +   D N    R D  +L +EW   H  S  V +R  L+  A DP+ T YL+GL       FD  R P  DP+L+DM   A+ +L +N + GF+L +EGGRID  HH   A  AL+E V  D  +E       ++  +TL +VTADHSH  S  GY  RG  I GL+     D  PY +I Y NG  Y         G   R  +T  E     +R  +A   KSE+HGG+DVAIFA GP +HL H   EQ +IAHVMA + CL  YT+
Sbjct:   25 EENPAFWNKKA-AEALDAAKKLQPIQTSAKNLIIFLGDGMGVPTVTATRILKGQLEGHL--GPETPLAMDRFPYMALSKTYSVDRQVPDSASTATAYLCGVKTNYKTIGL-SAAARFDQCNTTFGNEVFSVMYRAKKAGKSVGVVTTTRVQHASPSGTYVHTVNRNWYGDADMPASALREGCKDIATQLISN---MDINVILGGGRKYMFPAGTP-----DPEYPNDANETGTRLDGRNLVQEWLSKHQGSQYVWNREQLIQKAQDPSVT-YLMGLFEPVDTKFDIQRDPLMDPSLKDMTETAVKVLSRNPK-GFYLFVEGGRIDRGHHLGTAYLALTEAVMFDLAIERASQLTSER--DTLTIVTADHSHVFSFGGYTLRGTSIFGLAPLNALDGKPYTSILYGNGPGYV--------GTGERPNVTAAESSGSSYRRQAAVPVKSETHGGEDVAIFARGPQAHLVHGVQEQNYIAHVMASAGCLEPYTD 491          

BLAST of alkaline phosphatase 4 vs. nr
Match: gi|1070179338|ref|XP_018348561.1| (PREDICTED: alkaline phosphatase 4 [Trachymyrmex septentrionalis])

HSP 1 Score: 353.214 bits (905), Expect = 1.627e-112
Identity = 211/531 (39.74%), Postives = 298/531 (56.12%), Query Frame = 0

Query:   10 MKHLILILSYFCQLSVTFHHHGPIARHASSSNRDVAAQEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTA-QEVSTILSWAQEGGKDT-------------EGLH-----------------------DIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPD-GNGNVT-RRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            MK  IL+L        TF  H    R  S+        EDM +W + GQ  + + LTE   N  +AKNVIIF+GDGM + T+T+ RI+ GQ+NG   S E+  L+FE+FP+   +KTY+ D Q+PDSA TA+A+FSGVKTK+  +G D+          +    +++T+  WAQ+ G DT              GL+                       DIA QLV  EPG   KVI+GGG       +    ++M  K   DE+ NC R+D  +LA EW+E HPN  VV +   L+++D + T ++LG+   SH+ +   +  E P+L +M  +AI +LKKN + GF L++E G+ID AHH  WA+ AL E   +D+ V+     L   ++ETLI+VTADHSH+ ++NGY ARGNDI G   +     +PY T+SYANG  ++YH   D  N N T RRV       D  +R  +  + K ESHGG+DV ++ATGP+SHLF  T EQ +IAH +A+++C  F    +HC
Sbjct:    1 MKCYILLLCI-----ATFLRHSEALRKGSTD------YEDMSFWLKSGQETLHKILTEQK-NENRAKNVIIFIGDGMGLSTITSGRIYVGQLNG--QSGEEYQLSFEKFPNTALAKTYNVDKQVPDSAGTATAIFSGVKTKYRMVGLDARAQVSKCDEQINELSKLTTVADWAQQSGMDTGFVTTTRITHATPAGLYAHINHRDWECDSEIPKKQRHCVKDIARQLVHDEPGNQFKVIMGGGL-----SEMGMNVKMPLKKS-DESSNCQRSDGRNLANEWQEVHPNGRVVTNTYDLMSIDISNTSHILGVFSPSHMPYHAVKDDETPSLANMTTQAIKLLKKN-KNGFLLMVESGKIDIAHHGNWAKLALRELYELDEAVKVAQRLL--NMEETLIIVTADHSHSFTLNGYPARGNDILGFGNK-KPSQIPYETLSYANGPGFFYHRRNDTKNVNETWRRVEDDSTRNDPFYRHFAGKFLKDESHGGEDVGVYATGPYSHLFRGTFEQNYIAHAVAYAAC--FKDWPSHC 505          

BLAST of alkaline phosphatase 4 vs. nr
Match: gi|1068403989|ref|XP_018049365.1| (PREDICTED: alkaline phosphatase 4-like [Atta colombica] >gi|1068403992|ref|XP_018049366.1| PREDICTED: alkaline phosphatase 4-like [Atta colombica])

HSP 1 Score: 352.058 bits (902), Expect = 3.485e-112
Identity = 210/531 (39.55%), Postives = 297/531 (55.93%), Query Frame = 0

Query:   10 MKHLILILSYFCQLSVTFHHHGPIARHASSSNRDVAAQEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTA-QEVSTILSWAQEGGKDT-------------EGLH-----------------------DIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPD-GNGNVTRRVLTQEEVEDIEF-RSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            MK  IL+L        TF  H   A H  S+N      EDM +W + GQ  + + LTE   N  +AKNVIIF+GDGM + T+T+ RI+ GQ+NG   S E+  L+FE+FP+   +KTY+ D Q+PDSA TA+A+FSGVKTK+  +G D+          +    +++T+  WAQ+ GKDT              GL+                       DIA QLV  EPG   KVI+GGG        K  + +       DE+ NC R+D  +LA EW+++HP   VV D   L+++D   T ++LG+   SH+ +   +  E P+L +M  +AI +LKKN + GF L++E G+ID AHH  WA+ AL E   +D+ V+     L   ++ETLI+VTADHSH+ ++NGY ARGNDI G   +     +PY T+SYANG  ++YH   D  N N T R +  +   D  F R  +  + K ESHGG+DV ++A GP+SHLF  T EQ +IAH +A+++C  F    +HC
Sbjct:    1 MKCYILLLCI-----ATFLRHSE-ALHKGSTN-----YEDMSFWLKSGQETLHKILTEQK-NENRAKNVIIFIGDGMGLSTITSGRIYVGQLNG--QSGEEYQLSFEKFPNTALAKTYNVDKQVPDSAGTATAIFSGVKTKYRMVGLDARAQVSKCDEQINELSKLTTVADWAQQSGKDTGFVTTTRITHATPAGLYAHINHRDWECDSEIPKKQRHCVKDIARQLVHDEPGSQFKVIMGGGLSEMGMNVKMPLKKS------DESSNCERSDGRNLANEWQQSHPKGKVVTDTYDLMSIDINNTSHILGVFSSSHMPYHAVKDDETPSLANMTTQAIRLLKKN-KNGFLLMVESGKIDIAHHGNWAKLALRELYELDEAVKVAQRLL--NIEETLIIVTADHSHSFTLNGYPARGNDILGFGNK-KASQIPYETLSYANGPGFFYHRRNDTKNVNETWRRVENDSTRDEPFYRHFAGKFLKDESHGGEDVGVYAIGPYSHLFRGTFEQNYIAHAVAYAAC--FKDWPSHC 505          

BLAST of alkaline phosphatase 4 vs. nr
Match: gi|1070211170|ref|XP_018374762.1| (PREDICTED: alkaline phosphatase 4 [Trachymyrmex cornetzi])

HSP 1 Score: 350.132 bits (897), Expect = 1.996e-111
Identity = 209/531 (39.36%), Postives = 298/531 (56.12%), Query Frame = 0

Query:   10 MKHLILILSYFCQLSVTFHHHGPIARHASSSNRDVAAQEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTA-QEVSTILSWAQEGGKDT-------------EGLH-----------------------DIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPD-GNGNVTRRVLTQEEVEDIEF-RSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            MK  IL+L        TF  H    R  S+        EDM +W + GQ  + + LTE   N  +AKNVIIF+GDGM + T+T+ RI+ GQ+NG   S E+  L+FE+FP+   +KTY+ D Q+PDSA TA+A+FSGVKTK+  +G D+    +     L    +++T+  WAQ+ G DT              GL+                       DIA QLV  EPG   KVI+GGG       D K  L+      +DE+ NC R+D  +LA EW+E+HP   VV +   L+++D + T ++LG+   SH+ +   +  E P+L +M  +AI +LKKN + GF L++E G+ID AHH  WA+ AL E   +D+ V+     L   ++ETLI+VTADHSH+ ++NGY ARGNDI G   +     +PY T+SYANG  ++YH   D  + N T R +  +   D  F R  +  + K ESHGG+DV ++A GP+SHLF  T EQ +IAH +A+++C  F    +HC
Sbjct:    1 MKCYILLLCI-----ATFLRHSEALRKGSTD------YEDMSFWLKSGQETLHKILTEQE-NENRAKNVIIFIGDGMGLSTITSGRIYVGQLNGQ--SGEEYQLSFEKFPNTALAKTYNVDKQVPDSAGTATAIFSGVKTKYRMVGLDARAQVNKCDEQLNELSKLTTVADWAQQSGMDTGFVTTTRITHATPAGLYAHINHRDWECDSEIPKKQRHCVKDIARQLVHDEPGSQFKVIMGGGLSEM-GMDVKMPLKK-----FDESSNCKRSDGRNLANEWQESHPKGRVVTNTYDLMSIDISNTSHILGVFSPSHMPYHAVKDDETPSLANMTTQAIRLLKKN-KNGFLLMVESGKIDIAHHGNWAKLALRELYELDEAVKVAQRLL--NMEETLIIVTADHSHSFTLNGYPARGNDILGFGNK-KASQIPYETLSYANGPGFFYHRRNDTKDVNETWRRVEDDSTRDEPFYRHFAGKFLKDESHGGEDVGVYAIGPYSHLFRGTFEQNYIAHAVAYAAC--FKDWPSHC 505          

BLAST of alkaline phosphatase 4 vs. nr
Match: gi|780676231|ref|XP_011696490.1| (PREDICTED: alkaline phosphatase 4 [Wasmannia auropunctata] >gi|780676235|ref|XP_011696491.1| PREDICTED: alkaline phosphatase 4 [Wasmannia auropunctata])

HSP 1 Score: 349.362 bits (895), Expect = 3.791e-111
Identity = 207/531 (38.98%), Postives = 299/531 (56.31%), Query Frame = 0

Query:   10 MKHLILILSYFCQLSVTFHHHGPIARHASSSNRDVAAQEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTA-QEVSTILSWAQEGGKDT-------------EGLH-----------------------DIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPDGNG-NVTRRVLTQEEVEDIEF-RSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            MK  IL+L   C ++V         R + +  +     EDM +W + GQ  + R LTE   N  +AKNVIIF+GDGM + T+T+ RI+ GQ+NG   S E+  L+FE+FP+   +KTY+ D Q+PDSA TA+A+FSGVKTK+  +G D+  ++      +    +VST+  WAQ+ GKDT              GL+                       DIA QLV  EPG   KVI+GGG            +  + K    ++ +C R+D  +L KEW+  HP    V +   L+++D + T ++LG+   SH+ +   + PE P+L +M  +AI +LKKN + GFFL++E G+ID AHH  WA+ AL E   +D+ V+A    L   ++ETLI+VTADHSH+ ++NGY  RGNDI G   +    + PY T+SYANG  ++YH   D N  N T R L  +   +  F R  +  Y K ESHGG+DV ++A GP+SHLF  T EQ +IAH +A+++CL  +   +HC
Sbjct:    1 MKRCILLL---CIVAVL--------RDSEALRKGSTDYEDMSFWLKSGQETLHRILTEPK-NENRAKNVIIFIGDGMGLSTITSGRIYVGQLNGY--SGEEYQLSFEKFPNAALAKTYNVDKQVPDSAGTATAIFSGVKTKYRLIGLDARAEYSKCDKKINELSKVSTVADWAQQSGKDTGFVTTTRITHATPAGLYAHVNHRDWECDSDVPKNQRFCIKDIAAQLVEDEPGNHFKVIMGGG---------GEQMGTDMKMIKPDSNSCTRSDGRNLIKEWQVLHPEGKAVTNIHDLMSIDISNTSHILGVFSASHMPYHAVKSPETPSLTNMTTQAIRMLKKN-KNGFFLMVESGKIDIAHHGNWAQLALRELYELDEAVKAATRLL--NMEETLIIVTADHSHSFTLNGYPERGNDILGFGNKKLSQE-PYETLSYANGPGFFYHRRNDSNNVNETWRRLDNDTTRNEPFYRHFAGKYLKDESHGGEDVGVYAIGPYSHLFRGTFEQNYIAHAVAYAACLKNW--PSHC 502          

BLAST of alkaline phosphatase 4 vs. nr
Match: gi|1009415986|gb|KYN34787.1| (Alkaline phosphatase 4 [Trachymyrmex septentrionalis])

HSP 1 Score: 346.665 bits (888), Expect = 2.296e-110
Identity = 199/491 (40.53%), Postives = 284/491 (57.84%), Query Frame = 0

Query:   50 MEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTA-QEVSTILSWAQEGGKDT-------------EGLH-----------------------DIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPD-GNGNVT-RRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            M +W + GQ  + + LTE   N  +AKNVIIF+GDGM + T+T+ RI+ GQ+NG   S E+  L+FE+FP+   +KTY+ D Q+PDSA TA+A+FSGVKTK+  +G D+          +    +++T+  WAQ+ G DT              GL+                       DIA QLV  EPG   KVI+GGG       +    ++M  K   DE+ NC R+D  +LA EW+E HPN  VV +   L+++D + T ++LG+   SH+ +   +  E P+L +M  +AI +LKKN + GF L++E G+ID AHH  WA+ AL E   +D+ V+     L   ++ETLI+VTADHSH+ ++NGY ARGNDI G   +     +PY T+SYANG  ++YH   D  N N T RRV       D  +R  +  + K ESHGG+DV ++ATGP+SHLF  T EQ +IAH +A+++C  F    +HC
Sbjct:    1 MSFWLKSGQETLHKILTEQK-NENRAKNVIIFIGDGMGLSTITSGRIYVGQLNG--QSGEEYQLSFEKFPNTALAKTYNVDKQVPDSAGTATAIFSGVKTKYRMVGLDARAQVSKCDEQINELSKLTTVADWAQQSGMDTGFVTTTRITHATPAGLYAHINHRDWECDSEIPKKQRHCVKDIARQLVHDEPGNQFKVIMGGGL-----SEMGMNVKMPLKKS-DESSNCQRSDGRNLANEWQEVHPNGRVVTNTYDLMSIDISNTSHILGVFSPSHMPYHAVKDDETPSLANMTTQAIKLLKKN-KNGFLLMVESGKIDIAHHGNWAKLALRELYELDEAVKVAQRLL--NMEETLIIVTADHSHSFTLNGYPARGNDILGFGNK-KPSQIPYETLSYANGPGFFYHRRNDTKNVNETWRRVEDDSTRNDPFYRHFAGKFLKDESHGGEDVGVYATGPYSHLFRGTFEQNYIAHAVAYAAC--FKDWPSHC 476          

BLAST of alkaline phosphatase 4 vs. nr
Match: gi|746850725|ref|XP_011055854.1| (PREDICTED: alkaline phosphatase 4-like [Acromyrmex echinatior])

HSP 1 Score: 345.125 bits (884), Expect = 2.206e-109
Identity = 198/506 (39.13%), Postives = 288/506 (56.92%), Query Frame = 0

Query:   35 RHASSSNRDVAAQEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTA-QEVSTILSWAQEGGKDT-------------EGLH-----------------------DIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPD-GNGNVTRRVLTQEEVEDIEF-RSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            RH+ +  +     EDM +W + GQ  + + LTE   N  +AKNVIIF+GDGM + T+T+ RI+ GQ+NG   S E+  L+FE+FP+   +KTY+ D Q+PDSA TA+A+FSGVKTK+  +G D+          +    +++T+  WAQ+ G DT              GL+                       DIA QLV  EPG   K+I+GGG       D K  L+       DE+ NC R+D  +LA EW+E HP   +V +   L+++D + T ++LG+   SH+ +   +  E P+L +M  +AI +LKKN + GF L++E G+ID AHH  WA+ AL E   +D+ V+     L   ++ETLI+VTADHSH+ ++NGY ARGNDI G   +     +PY T+SYANG  ++YH   D  + N T R +  +   D  F R  +  + K ESHGG+DV ++A GP+SHLF  T EQ +IAH +A+++C  F    +HC
Sbjct:   15 RHSEALRKGSMDYEDMSFWLKSGQETLHKILTEQK-NENRAKNVIIFIGDGMGLSTITSGRIYVGQLNGQ--SGEEYQLSFEKFPNTALAKTYNVDKQVPDSAGTATAIFSGVKTKYRLIGLDARAQVSKCDEQINELSKLTTVADWAQQSGMDTGFVTTTRITHATPAGLYAHINHRDWECDSEIPKKQRHCIKDIARQLVHNEPGNQFKIIMGGGLSEM-GMDVKMPLKKS-----DESSNCERSDGRNLANEWQEVHPKGRIVTNTYDLMSIDISNTSHILGVFSPSHMPYHAVKDDETPSLANMTTQAIRLLKKN-KNGFLLMVESGKIDIAHHGNWAKLALRELYELDEAVKVAQRLL--NMEETLIIVTADHSHSFTLNGYPARGNDILGFGNK-KASQIPYETLSYANGPGFFYHRRNDTKDVNETWRRIEDDSTRDEPFYRHFAGKFLKDESHGGEDVGVYAIGPYSHLFRGTFEQNYIAHAVAYAAC--FKDWPSHC 505          

BLAST of alkaline phosphatase 4 vs. nr
Match: gi|1069692131|ref|XP_018305958.1| (PREDICTED: alkaline phosphatase 4 [Trachymyrmex zeteki])

HSP 1 Score: 344.354 bits (882), Expect = 4.671e-109
Identity = 196/506 (38.74%), Postives = 284/506 (56.13%), Query Frame = 0

Query:   35 RHASSSNRDVAAQEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTA-QEVSTILSWAQEGGKDT-------------EGLH-----------------------DIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPDGN--GNVTRRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            RH+ +  +     EDM +W + GQ E L  +     N  +AKNVIIF+GDGM + T+T+ RI+ GQ+NG   S E+  L+FE+FP+   +KTY+ D Q+PDSA TA+A+FSGVKTK+  +G D+          +    +++T+  WAQ+ G DT              GL+                       DIA QLV  EPG   KVI+GGG       D K  L+      +DE+ NC R+D  +LA EW+ENHP   VV +   L+++D + T ++LG+   SH+ +   +  E P+L +M  +AI +LKKN + GF L++E G+ID AHH  WA+ AL E   +D+ V+     L   ++ETLI+VTADHSH+ ++NGY  RGNDI G   +     +PY T+SYANG  ++YH   D        RRV       +  +R  +  + K ESHGG+DV ++A GP++HLF  T EQ +IAH +A+++C  F    +HC
Sbjct:   15 RHSEALRKGSTDYEDMSFWLKSGQ-ETLHKILAEQKNENRAKNVIIFIGDGMGLSTITSGRIYVGQLNGQ--SGEEYQLSFEKFPNTALAKTYNVDKQVPDSAGTATAIFSGVKTKYRMVGLDARAQVSKCDEQINELSKLNTVADWAQQSGIDTGFVTTTRVTHATPAGLYAHINHRDWECDSAIPKKQRYCIKDIARQLVHDEPGSQFKVIMGGGLSEM-GMDVKMPLKK-----FDESSNCERSDGRNLANEWQENHPKGRVVTNTYDLMSIDISNTSHILGVFSPSHMPYHAVKDDETPSLANMTTQAIRLLKKN-KNGFLLMVESGKIDIAHHGNWAKLALRELYELDEAVKVAQRLL--NMEETLIIVTADHSHSFTLNGYPTRGNDILGFGNKK-ASQIPYETLSYANGPGFFYHRRNDTKDVNETWRRVEDDPTRGEPFYRHFAGKFLKDESHGGEDVGVYANGPYAHLFRGTFEQNYIAHAVAYAAC--FKDWPSHC 505          

BLAST of alkaline phosphatase 4 vs. nr
Match: gi|1009388536|gb|KYN10534.1| (Alkaline phosphatase 4 [Trachymyrmex cornetzi])

HSP 1 Score: 343.199 bits (879), Expect = 4.994e-109
Identity = 197/491 (40.12%), Postives = 284/491 (57.84%), Query Frame = 0

Query:   50 MEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTA-QEVSTILSWAQEGGKDT-------------EGLH-----------------------DIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPD-GNGNVTRRVLTQEEVEDIEF-RSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            M +W + GQ  + + LTE   N  +AKNVIIF+GDGM + T+T+ RI+ GQ+NG   S E+  L+FE+FP+   +KTY+ D Q+PDSA TA+A+FSGVKTK+  +G D+    +     L    +++T+  WAQ+ G DT              GL+                       DIA QLV  EPG   KVI+GGG       D K  L+      +DE+ NC R+D  +LA EW+E+HP   VV +   L+++D + T ++LG+   SH+ +   +  E P+L +M  +AI +LKKN + GF L++E G+ID AHH  WA+ AL E   +D+ V+     L   ++ETLI+VTADHSH+ ++NGY ARGNDI G   +     +PY T+SYANG  ++YH   D  + N T R +  +   D  F R  +  + K ESHGG+DV ++A GP+SHLF  T EQ +IAH +A+++C  F    +HC
Sbjct:    1 MSFWLKSGQETLHKILTEQE-NENRAKNVIIFIGDGMGLSTITSGRIYVGQLNGQ--SGEEYQLSFEKFPNTALAKTYNVDKQVPDSAGTATAIFSGVKTKYRMVGLDARAQVNKCDEQLNELSKLTTVADWAQQSGMDTGFVTTTRITHATPAGLYAHINHRDWECDSEIPKKQRHCVKDIARQLVHDEPGSQFKVIMGGGLSEM-GMDVKMPLKK-----FDESSNCKRSDGRNLANEWQESHPKGRVVTNTYDLMSIDISNTSHILGVFSPSHMPYHAVKDDETPSLANMTTQAIRLLKKN-KNGFLLMVESGKIDIAHHGNWAKLALRELYELDEAVKVAQRLL--NMEETLIIVTADHSHSFTLNGYPARGNDILGFGNK-KASQIPYETLSYANGPGFFYHRRNDTKDVNETWRRVEDDSTRDEPFYRHFAGKFLKDESHGGEDVGVYAIGPYSHLFRGTFEQNYIAHAVAYAAC--FKDWPSHC 476          

BLAST of alkaline phosphatase 4 vs. nr
Match: gi|826432143|ref|XP_012528560.1| (PREDICTED: alkaline phosphatase 4-like [Monomorium pharaonis])

HSP 1 Score: 343.199 bits (879), Expect = 6.893e-109
Identity = 202/531 (38.04%), Postives = 296/531 (55.74%), Query Frame = 0

Query:   10 MKHLILILSYFCQLSVTFHHHGPIARHASSSNRDVAAQEDMEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTA-QEVSTILSWAQEGGKDT-------------EGLH----------------------DIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPDGNGNVT---RRVLTQEEVEDIEFRSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            MK  IL+L   C ++        I R + +  +     E+M YW + GQ E L  + +   N  +AKNVIIF+GDGM + T+T+ RI+ GQ++G+  S E+  L FE+FP+   +KTY+ D Q+PDSA TA+A+FSGVKTK+  +G D+   ++     L    ++ST+  WAQ+ G DT              GL+                      DIA QLV  EPG   KVILGGG             +M  K    ++ +C R+D  +L KEW+  HP    V +   L++VD + T ++LG+    H+ F   + PE P+L +M ++AI +LKKN + GF L++E G+ID AHH  WA+ AL E   +D+ ++  +  +   ++ETLIVVTADHSH+ ++NGY  RGNDI G + +     +PY T+SYANG  ++YH   + N NV    RRV       +  +R  +  Y K ESHGG+DV ++A GP+SHLF  T EQ +IAH +A+++C  F    +HC
Sbjct:    1 MKRCILLL---CIVA--------ILRDSEALPKGSTNYEEMSYWLKSGQ-ETLHRILKEQKNENRAKNVIIFIGDGMGLSTITSGRIYVGQLHGH--SGEEYQLEFEKFPNTALAKTYNVDKQVPDSAGTATAIFSGVKTKYRLIGLDARAPYNKCDKELNRLSKLSTVADWAQQSGMDTGFVTTTRITHATPAGLYAHVNHRDWECDTAIPKKEQLCKDIARQLVEDEPGNQFKVILGGG-----------AQQMGMKMNESDSSSCVRSDGKNLVKEWQAKHPKGRAVSNMQELMSVDISDTSHILGVFSPGHMPFHAVKSPEVPSLANMTMQAIRMLKKN-KNGFLLMVESGKIDTAHHGNWAQLALRELYELDEAIKVALRLV--NMEETLIVVTADHSHSFTLNGYPERGNDILGFANK-KASQVPYETLSYANGPGFFYHRRNNSN-NVNETWRRVEDDPTRNEPFYRHFAGKYLKDESHGGEDVGVYAHGPFSHLFRGTFEQNYIAHAVAYAAC--FKDWPSHC 499          

BLAST of alkaline phosphatase 4 vs. nr
Match: gi|1070610535|ref|XP_018403093.1| (PREDICTED: alkaline phosphatase 4 [Cyphomyrmex costatus])

HSP 1 Score: 341.273 bits (874), Expect = 2.254e-108
Identity = 196/491 (39.92%), Postives = 280/491 (57.03%), Query Frame = 0

Query:   50 MEYWNQLGQGEILRSLTEATTNTKKAKNVIIFVGDGMSIPTLTAARIFKGQINGNMDSAEKEYLTFERFPHLGHSKTYDTDYQIPDSASTASAMFSGVKTKFYSLGYDSSIDHHDVTSMLTA-QEVSTILSWAQEGGKDT-------------EGLH-----------------------DIAYQLVRQEPGMSMKVILGGGFPAFFPKDKKAMLEMEEKTGYDENWNCYRNDSLDLAKEWEENHPNSAVVRDRSSLLAVDPATTDYLLGLLGRSHVAFDDARGPEDPTLEDMALKAIDILKKNSEEGFFLLIEGGRIDHAHHDVWARRALSETVAMDKTVEAVMAALGDQLDETLIVVTADHSHTMSINGYAARGNDIAGLSTEVYDDDLPYPTISYANGVNYYYHNIPD-GNGNVTRRVLTQEEVEDIEF-RSPSAAYKKSESHGGDDVAIFATGPWSHLFHTTHEQTHIAHVMAFSSCLGFYTNEAHC 501
            M +W + GQ  + + LTE   N  +AKNVIIF+GDGM + T+T+ RI+ GQ+NG   S E+  L FE+FP+   +KTY+ D Q+PDSA TA+A+FSGVKTK+  +G D+          L    +++T+  WAQ+ G DT              GL+                       DIA QL+  EPG   KVI+GGG       D K  L+      +DE+ +C R D  +LA EW+ENHP   VV +   L+++D + T ++LG+   SH+ +   +  E P+L +M  +AI +LKKN + GF L++E G+ID AHH  WA+ AL E   +D+ V+     L   ++ETLI+VTADHSH+ ++NGY  RGNDI G         +PY T+SYANG  ++YH   D  N N T R +  +   D  F R  +  + K ESHGG+DV ++A+GP+SHLF  T EQ +IAH +A+++C  F    +HC
Sbjct:    1 MSFWLKSGQETLHKILTEQK-NENRAKNVIIFIGDGMGLSTITSGRIYVGQLNG--QSGEEYQLAFEKFPNTALAKTYNVDKQVPDSAGTATAIFSGVKTKYRMVGLDARAQVSKCDEQLNELSKLTTVADWAQQSGMDTGFVTTTRITHATPAGLYAHINHRDWECDSAIPKKQRHCVKDIARQLIHNEPGNQFKVIMGGGLSEM-GMDVKMPLKK-----FDESSSCVRADGRNLANEWQENHPKGRVVTNTYDLMSIDISNTSHILGVFSPSHMPYHAVKDDEIPSLANMTTQAIRLLKKN-KNGFLLMVESGKIDIAHHGNWAKLALRELYELDEAVKVAQRLL--NMEETLIIVTADHSHSFTLNGYPMRGNDILGFGNR-KPSQIPYETLSYANGPGFFYHRRNDTKNVNETWRKVEDDPTRDEPFYRHFAGKFLKDESHGGEDVGVYASGPYSHLFRGTFEQNYIAHAVAYAAC--FKDWPSHC 476